LOCUS       DAMYXM010000001       136528 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.001, whole genome shotgun sequence.
ACCESSION   DAMYXM010000001 DAMYXM010000000
VERSION     DAMYXM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 136528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 136528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..136528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.001"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(143..2329)
                     /locus_tag="PNS21_000001"
     CDS             complement(143..2329)
                     /locus_tag="PNS21_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDI5157750.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            2547..3230
                     /locus_tag="PNS21_000002"
     CDS             2547..3230
                     /locus_tag="PNS21_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDI5157751.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3311..4030)
                     /locus_tag="PNS21_000003"
     CDS             complement(3311..4030)
                     /locus_tag="PNS21_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDI5157752.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4342..4548)
                     /locus_tag="PNS21_000004"
     CDS             complement(4342..4548)
                     /locus_tag="PNS21_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5157753.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            4793..5395
                     /locus_tag="PNS21_000005"
     CDS             4793..5395
                     /locus_tag="PNS21_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDI5157754.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            5536..6816
                     /locus_tag="PNS21_000006"
     CDS             5536..6816
                     /locus_tag="PNS21_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDI5157755.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            6829..7557
                     /locus_tag="PNS21_000007"
     CDS             6829..7557
                     /locus_tag="PNS21_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI5157756.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(7626..9149)
                     /locus_tag="PNS21_000008"
     CDS             complement(7626..9149)
                     /locus_tag="PNS21_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDI5157757.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            9401..10582
                     /locus_tag="PNS21_000009"
     CDS             9401..10582
                     /locus_tag="PNS21_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDI5157758.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKCLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(10642..12270)
                     /gene="treC"
                     /locus_tag="PNS21_000010"
     CDS             complement(10642..12270)
                     /gene="treC"
                     /locus_tag="PNS21_000010"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDI5157759.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12667..15378)
                     /locus_tag="PNS21_000011"
     CDS             complement(12667..15378)
                     /locus_tag="PNS21_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5157760.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(15388..16089)
                     /locus_tag="PNS21_000012"
     CDS             complement(15388..16089)
                     /locus_tag="PNS21_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5157761.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            16275..16874
                     /locus_tag="PNS21_000013"
     CDS             16275..16874
                     /locus_tag="PNS21_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDI5157762.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            16955..17968
                     /locus_tag="PNS21_000014"
     CDS             16955..17968
                     /locus_tag="PNS21_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDI5157763.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            18076..18993
                     /gene="rbsK"
                     /locus_tag="PNS21_000015"
     CDS             18076..18993
                     /gene="rbsK"
                     /locus_tag="PNS21_000015"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDI5157764.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            19021..19416
                     /gene="rbsD"
                     /locus_tag="PNS21_000016"
     CDS             19021..19416
                     /gene="rbsD"
                     /locus_tag="PNS21_000016"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDI5157765.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            19416..20789
                     /locus_tag="PNS21_000017"
     CDS             19416..20789
                     /locus_tag="PNS21_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDI5157766.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            21146..23263
                     /locus_tag="PNS21_000018"
     CDS             21146..23263
                     /locus_tag="PNS21_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDI5157767.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIPETTTRTVKA
                     ATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(23315..24355)
                     /locus_tag="PNS21_000019"
     CDS             complement(23315..24355)
                     /locus_tag="PNS21_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDI5157768.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(24632..26434)
                     /locus_tag="PNS21_000020"
     CDS             complement(24632..26434)
                     /locus_tag="PNS21_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5157769.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(26448..27200)
                     /locus_tag="PNS21_000021"
     CDS             complement(26448..27200)
                     /locus_tag="PNS21_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5157770.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(27209..27571)
                     /locus_tag="PNS21_000022"
     CDS             complement(27209..27571)
                     /locus_tag="PNS21_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDI5157771.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(27747..28619)
                     /locus_tag="PNS21_000023"
     CDS             complement(27747..28619)
                     /locus_tag="PNS21_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDI5157772.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(28670..29158)
                     /locus_tag="PNS21_000024"
     CDS             complement(28670..29158)
                     /locus_tag="PNS21_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDI5157773.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(29296..30087)
                     /locus_tag="PNS21_000025"
     CDS             complement(29296..30087)
                     /locus_tag="PNS21_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDI5157774.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(30142..30750)
                     /locus_tag="PNS21_000026"
     CDS             complement(30142..30750)
                     /locus_tag="PNS21_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDI5157775.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(30743..31060)
                     /locus_tag="PNS21_000027"
     CDS             complement(30743..31060)
                     /locus_tag="PNS21_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDI5157776.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(31216..31944)
                     /locus_tag="PNS21_000028"
     CDS             complement(31216..31944)
                     /locus_tag="PNS21_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDI5157777.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            32103..32984
                     /locus_tag="PNS21_000029"
     CDS             32103..32984
                     /locus_tag="PNS21_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI5157778.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(33221..34264)
                     /locus_tag="PNS21_000030"
     CDS             complement(33221..34264)
                     /locus_tag="PNS21_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDI5157779.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(34267..34935)
                     /locus_tag="PNS21_000031"
     CDS             complement(34267..34935)
                     /locus_tag="PNS21_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI5157780.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            35077..37413
                     /locus_tag="PNS21_000032"
     CDS             35077..37413
                     /locus_tag="PNS21_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI5157781.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGNI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(37528..37839)
                     /locus_tag="PNS21_000033"
     CDS             complement(37528..37839)
                     /locus_tag="PNS21_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5157782.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            38020..39495
                     /locus_tag="PNS21_000034"
     CDS             38020..39495
                     /locus_tag="PNS21_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDI5157783.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            39542..40126
                     /locus_tag="PNS21_000035"
     CDS             39542..40126
                     /locus_tag="PNS21_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDI5157784.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            40128..40547
                     /locus_tag="PNS21_000036"
     CDS             40128..40547
                     /locus_tag="PNS21_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HDI5157785.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(40828..41112)
                     /locus_tag="PNS21_000037"
     CDS             complement(40828..41112)
                     /locus_tag="PNS21_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HDI5157786.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(41464..42174)
                     /locus_tag="PNS21_000038"
     CDS             complement(41464..42174)
                     /locus_tag="PNS21_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HDI5157787.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(42171..43643)
                     /locus_tag="PNS21_000039"
     CDS             complement(42171..43643)
                     /locus_tag="PNS21_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDI5157788.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            43781..44254
                     /locus_tag="PNS21_000040"
     CDS             43781..44254
                     /locus_tag="PNS21_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDI5157789.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(44407..44958)
                     /locus_tag="PNS21_000041"
     CDS             complement(44407..44958)
                     /locus_tag="PNS21_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDI5157790.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            45063..45593
                     /locus_tag="PNS21_000042"
     CDS             45063..45593
                     /locus_tag="PNS21_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HDI5157791.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(46076..46519)
                     /locus_tag="PNS21_000043"
     CDS             complement(46076..46519)
                     /locus_tag="PNS21_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5157792.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(46535..47695)
                     /locus_tag="PNS21_000044"
     CDS             complement(46535..47695)
                     /locus_tag="PNS21_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDI5157793.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            48202..49902
                     /locus_tag="PNS21_000045"
     CDS             48202..49902
                     /locus_tag="PNS21_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5157794.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(50004..50837)
                     /locus_tag="PNS21_000046"
     CDS             complement(50004..50837)
                     /locus_tag="PNS21_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDI5157795.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(51141..53306)
                     /locus_tag="PNS21_000047"
     CDS             complement(51141..53306)
                     /locus_tag="PNS21_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDI5157796.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            53566..55860
                     /locus_tag="PNS21_000048"
     CDS             53566..55860
                     /locus_tag="PNS21_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDI5157797.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            55853..56518
                     /gene="pgmB"
                     /locus_tag="PNS21_000049"
     CDS             55853..56518
                     /gene="pgmB"
                     /locus_tag="PNS21_000049"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDI5157798.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            56511..57518
                     /locus_tag="PNS21_000050"
     CDS             56511..57518
                     /locus_tag="PNS21_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDI5157799.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            57540..58559
                     /locus_tag="PNS21_000051"
     CDS             57540..58559
                     /locus_tag="PNS21_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI5157800.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(58840..60207)
                     /gene="nhaC"
                     /locus_tag="PNS21_000052"
     CDS             complement(58840..60207)
                     /gene="nhaC"
                     /locus_tag="PNS21_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDI5157801.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(60316..61737)
                     /locus_tag="PNS21_000053"
     CDS             complement(60316..61737)
                     /locus_tag="PNS21_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI5157802.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(61980..63857)
                     /gene="tdc"
                     /locus_tag="PNS21_000054"
     CDS             complement(61980..63857)
                     /gene="tdc"
                     /locus_tag="PNS21_000054"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDI5157803.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(64148..65404)
                     /locus_tag="PNS21_000055"
     CDS             complement(64148..65404)
                     /locus_tag="PNS21_000055"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDI5157804.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(65516..65721)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            65984..67285
                     /locus_tag="PNS21_000056"
     CDS             65984..67285
                     /locus_tag="PNS21_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDI5157805.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(67354..67953)
                     /locus_tag="PNS21_000057"
     CDS             complement(67354..67953)
                     /locus_tag="PNS21_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI5157806.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            68207..69088
                     /locus_tag="PNS21_000058"
     CDS             68207..69088
                     /locus_tag="PNS21_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5157807.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            69101..70711
                     /locus_tag="PNS21_000059"
     CDS             69101..70711
                     /locus_tag="PNS21_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HDI5157808.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(70788..71447)
                     /locus_tag="PNS21_000060"
     CDS             complement(70788..71447)
                     /locus_tag="PNS21_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDI5157809.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(71730..72395)
                     /locus_tag="PNS21_000061"
     CDS             complement(71730..72395)
                     /locus_tag="PNS21_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDI5157810.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(72542..73147)
                     /gene="dhaL"
                     /locus_tag="PNS21_000062"
     CDS             complement(72542..73147)
                     /gene="dhaL"
                     /locus_tag="PNS21_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDI5157811.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(73175..74185)
                     /gene="dhaK"
                     /locus_tag="PNS21_000063"
     CDS             complement(73175..74185)
                     /gene="dhaK"
                     /locus_tag="PNS21_000063"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDI5157812.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(74160..74606)
                     /gene="dhaM"
                     /locus_tag="PNS21_000064"
     CDS             complement(74160..74606)
                     /gene="dhaM"
                     /locus_tag="PNS21_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDI5157813.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            74709..75029
                     /locus_tag="PNS21_000065"
     CDS             74709..75029
                     /locus_tag="PNS21_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDI5157814.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(75141..76010)
                     /locus_tag="PNS21_000066"
     CDS             complement(75141..76010)
                     /locus_tag="PNS21_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDI5157815.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(76106..77410)
                     /locus_tag="PNS21_000067"
     CDS             complement(76106..77410)
                     /locus_tag="PNS21_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDI5157816.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(77407..77916)
                     /locus_tag="PNS21_000068"
     CDS             complement(77407..77916)
                     /locus_tag="PNS21_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5157817.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(78091..80226)
                     /locus_tag="PNS21_000069"
     CDS             complement(78091..80226)
                     /locus_tag="PNS21_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI5157818.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(80309..81964)
                     /locus_tag="PNS21_000070"
     CDS             complement(80309..81964)
                     /locus_tag="PNS21_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDI5157819.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(81966..82280)
                     /locus_tag="PNS21_000071"
     CDS             complement(81966..82280)
                     /locus_tag="PNS21_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDI5157820.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            82450..82710
                     /locus_tag="PNS21_000072"
     CDS             82450..82710
                     /locus_tag="PNS21_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDI5157821.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            82724..83038
                     /locus_tag="PNS21_000073"
     CDS             82724..83038
                     /locus_tag="PNS21_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDI5157822.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(83768..84322)
                     /locus_tag="PNS21_000074"
     CDS             complement(83768..84322)
                     /locus_tag="PNS21_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDI5157823.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(84448..86376)
                     /locus_tag="PNS21_000075"
     CDS             complement(84448..86376)
                     /locus_tag="PNS21_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDI5157824.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(86682..87398)
                     /locus_tag="PNS21_000076"
     CDS             complement(86682..87398)
                     /locus_tag="PNS21_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDI5157825.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(87399..89222)
                     /gene="glpO"
                     /locus_tag="PNS21_000077"
     CDS             complement(87399..89222)
                     /gene="glpO"
                     /locus_tag="PNS21_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HDI5157826.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(89236..90732)
                     /gene="glpK"
                     /locus_tag="PNS21_000078"
     CDS             complement(89236..90732)
                     /gene="glpK"
                     /locus_tag="PNS21_000078"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDI5157827.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(90868..92328)
                     /locus_tag="PNS21_000079"
     CDS             complement(90868..92328)
                     /locus_tag="PNS21_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5157828.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(92318..93613)
                     /locus_tag="PNS21_000080"
     CDS             complement(92318..93613)
                     /locus_tag="PNS21_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDI5157829.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(93945..94622)
                     /locus_tag="PNS21_000081"
     CDS             complement(93945..94622)
                     /locus_tag="PNS21_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HDI5157830.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(94652..95743)
                     /locus_tag="PNS21_000082"
     CDS             complement(94652..95743)
                     /locus_tag="PNS21_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDI5157831.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(95844..96278)
                     /locus_tag="PNS21_000083"
     CDS             complement(95844..96278)
                     /locus_tag="PNS21_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDI5157832.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(96862..98319)
                     /gene="ascB"
                     /locus_tag="PNS21_000084"
     CDS             complement(96862..98319)
                     /gene="ascB"
                     /locus_tag="PNS21_000084"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDI5157833.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(98316..100211)
                     /locus_tag="PNS21_000085"
     CDS             complement(98316..100211)
                     /locus_tag="PNS21_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDI5157834.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(100269..102044)
                     /locus_tag="PNS21_000086"
     CDS             complement(100269..102044)
                     /locus_tag="PNS21_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDI5157835.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(102094..103590)
                     /locus_tag="PNS21_000087"
     CDS             complement(102094..103590)
                     /locus_tag="PNS21_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDI5157836.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(103784..105406)
                     /locus_tag="PNS21_000088"
     CDS             complement(103784..105406)
                     /locus_tag="PNS21_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDI5157837.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(105855..107168)
                     /locus_tag="PNS21_000089"
     CDS             complement(105855..107168)
                     /locus_tag="PNS21_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDI5157838.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(107413..107637)
                     /locus_tag="PNS21_000090"
     CDS             complement(107413..107637)
                     /locus_tag="PNS21_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5157839.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(107657..108079)
                     /locus_tag="PNS21_000091"
     CDS             complement(107657..108079)
                     /locus_tag="PNS21_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDI5157840.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(108222..108932)
                     /locus_tag="PNS21_000092"
     CDS             complement(108222..108932)
                     /locus_tag="PNS21_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5157841.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(108950..109447)
                     /locus_tag="PNS21_000093"
     CDS             complement(108950..109447)
                     /locus_tag="PNS21_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5157842.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(109484..109936)
                     /locus_tag="PNS21_000094"
     CDS             complement(109484..109936)
                     /locus_tag="PNS21_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDI5157843.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(110071..110676)
                     /locus_tag="PNS21_000095"
     CDS             complement(110071..110676)
                     /locus_tag="PNS21_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDI5157844.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(111126..111746)
                     /locus_tag="PNS21_000096"
     CDS             complement(111126..111746)
                     /locus_tag="PNS21_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI5157845.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(111748..112617)
                     /locus_tag="PNS21_000097"
     CDS             complement(111748..112617)
                     /locus_tag="PNS21_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDI5157846.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(112640..113047)
                     /locus_tag="PNS21_000098"
     CDS             complement(112640..113047)
                     /locus_tag="PNS21_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDI5157847.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(113066..114355)
                     /locus_tag="PNS21_000099"
     CDS             complement(113066..114355)
                     /locus_tag="PNS21_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDI5157848.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(114382..114684)
                     /locus_tag="PNS21_000100"
     CDS             complement(114382..114684)
                     /locus_tag="PNS21_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDI5157849.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(114709..116802)
                     /locus_tag="PNS21_000101"
     CDS             complement(114709..116802)
                     /locus_tag="PNS21_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDI5157850.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(117291..118055)
                     /locus_tag="PNS21_000102"
     CDS             complement(117291..118055)
                     /locus_tag="PNS21_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HDI5157851.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(118166..119035)
                     /gene="mdcB"
                     /locus_tag="PNS21_000103"
     CDS             complement(118166..119035)
                     /gene="mdcB"
                     /locus_tag="PNS21_000103"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HDI5157852.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(119060..120136)
                     /locus_tag="PNS21_000104"
     CDS             complement(119060..120136)
                     /locus_tag="PNS21_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDI5157853.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(120160..120753)
                     /locus_tag="PNS21_000105"
     CDS             complement(120160..120753)
                     /locus_tag="PNS21_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HDI5157854.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(120757..122433)
                     /gene="mdcD"
                     /locus_tag="PNS21_000106"
     CDS             complement(120757..122433)
                     /gene="mdcD"
                     /locus_tag="PNS21_000106"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HDI5157855.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(122423..122722)
                     /locus_tag="PNS21_000107"
     CDS             complement(122423..122722)
                     /locus_tag="PNS21_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HDI5157856.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(122824..124470)
                     /gene="mdcA"
                     /locus_tag="PNS21_000108"
     CDS             complement(122824..124470)
                     /gene="mdcA"
                     /locus_tag="PNS21_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HDI5157857.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(124477..125388)
                     /locus_tag="PNS21_000109"
     CDS             complement(124477..125388)
                     /locus_tag="PNS21_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDI5157858.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            125508..126365
                     /locus_tag="PNS21_000110"
     CDS             125508..126365
                     /locus_tag="PNS21_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI5157859.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(126552..126914)
                     /locus_tag="PNS21_000111"
     CDS             complement(126552..126914)
                     /locus_tag="PNS21_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDI5157860.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            127260..128699
                     /locus_tag="PNS21_000112"
     CDS             127260..128699
                     /locus_tag="PNS21_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5157861.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(128706..131258)
                     /locus_tag="PNS21_000113"
     CDS             complement(128706..131258)
                     /locus_tag="PNS21_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDI5157862.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPKGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYELGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(131372..132703)
                     /locus_tag="PNS21_000114"
     CDS             complement(131372..132703)
                     /locus_tag="PNS21_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI5157863.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(132669..134147)
                     /locus_tag="PNS21_000115"
     CDS             complement(132669..134147)
                     /locus_tag="PNS21_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI5157864.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(134452..136287)
                     /gene="tdc"
                     /locus_tag="PNS21_000116"
     CDS             complement(134452..136287)
                     /gene="tdc"
                     /locus_tag="PNS21_000116"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDI5157865.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        39903 a        28388 c        23820 g        44417 t
ORIGIN      
        1 acctcccttt tccgaataaa ctgtgttcca gaaatagctt cttcgaaaat aagctgaaat
       61 ttcacaaaaa tttgagaagc aattttcgta cattccttct tctttcttga aactgaccac
      121 ttctgtcaca ccctctcttc tatcactttc cttttttcac acgactcccg gtttctttga
      181 taaatctcga tactttcaat tttcttgaat ttttcatttg aggtacacat agatacagac
      241 gatttcttgc tctagtcatc gcaacataaa atagtcttcg ttcttcttct atcgtcgtca
      301 ctggttctgc ttttatttca ttaaagtcga ttactttctt ttctgtcgct ccgttataag
      361 gcaaacttcc ttcgttacaa ccaatcaaaa agacatttcg gtaactaaga cctttacttt
      421 tgtgaatgct ctggatacat agtgcatcaa gttttccttg tctgtaagca atcaagcggg
      481 tttttaattc tttagctgtc gcttgggttt cttgcgataa ggcttggagt cttctgaaag
      541 tagcatgctg ctttacttct tctcgtatcc attcttgatc actctggtcg atttccttag
      601 ctatacgtgg cagatgaaga tagctttgaa caagcgaata gatttcttga tgctggtctg
      661 ttttacgcaa cgcttcctca aactctcgga tttctgcaga tttcgatttt aaattggggc
      721 ttatcgacaa aatataatct gcaacggcta tattttgata tttaggagaa aagtattgat
      781 cgcattcact ttttttcagg tagcagtctg ccatcagctg ccggagcaat tgctggtagg
      841 cctgattttt cttttgcaga ttcagtgctg tttgccaatg aacaattttt tgatagaaat
      901 gttggaaaga cttgatacca aagatacctt catatggact atctgcttcc agtacaaaag
      961 gaatattcgt tttcagcaga gattggacaa gctgctgcgt ttgtgaagct gatcggtata
     1021 atatgattga ttccttatat ttcccatttt tttgaataaa tgtctgaatt tgtttgatta
     1081 gccactgact ttctgcttta gtatcagaaa acaaataggc ttccataaca cctgcttctt
     1141 tttgtgctgg aatcatcgcc tttggtatcc gatcattttt attatagctg atcaatctat
     1201 ttgctccttg gacaatagct gcccgagaac gataattagt taataagaaa agagttttgg
     1261 taggatattc tttttcaaag ttcttgatgt atgctggctc tgatcctctg aatccataga
     1321 tactctgatc atcatctcca acaacaatca acttgatcct gttttccttt gtcatccgtt
     1381 tcacgatttc ccattgaagt ggattagtat cttgaaattc atcaatgaat agataatcat
     1441 atttttggcc gatataatgc tggatctcct cattctccaa agcgcttttc agattttgga
     1501 gtacatcatt gaactctagc aattgctggc ttcttttttg ttcttggagt tccgtgagca
     1561 atccatacag tcgttctgat atcggctgtc gtgcattctt atcaatggaa tcatattttt
     1621 ctttaactaa ctgaaccatt tcttctactg aaaggttcat attctgccaa tagtctaacc
     1681 gagaagcaat cgttttttct ccatattgat caaatatata aagattatat ttacgtatca
     1741 gctgttgcaa aacagcattc actttttgtg cagtatcctt tgaaaagtct ccttgtattc
     1801 cttgcattgc tacttcagga aattttcgac aaatttcatt gtatatccta aaaaagatac
     1861 tatggaatgt tccgatatgg acagcggttt ttctctggct gaaattatct actcgttttt
     1921 ttagttcatt tgcagcgttc cttgtaaaag tgatcattag tatgcgttct tcgtctactt
     1981 gtttttgcgt catcagataa acagcttttg cacagattgt ttgcgttttg ccgctcccag
     2041 ctcctgctat gattcgaaga tgtttcttca tatcatatgt gatagctgct ttttgttctt
     2101 gattgaagcg attataggaa cgtttctcca tcattttaaa gaaagagctg tttccttcac
     2161 tttcttttac ttgtacttct tcctcaataa gtccgggtgc ttcgccataa gccgcttcat
     2221 aggtagcaat gatttcttct tgtagccgaa agacttgttg aagcttttta tacttgttct
     2281 cccagcgttt caattctttt tgtaatcgga gaatttcttt ttcattcaac tgattgattt
     2341 ccttcccatc tttcttcttt gaatttgttt atttcaagtt ggttatttaa agtttcgttg
     2401 tctatttttt ctttatacta acagaccttt ctaaaaatga aaatgggaag cgatatgatt
     2461 ttagttttct gaaaattttt cctttttttc aatcgtttat gctaagataa ggaagacaaa
     2521 aatattcata aaaggtgatg aaaaaaatgc agacttcctt tcacgagggg cgtacaatag
     2581 gacttttgct gacatttatc ggaggagcca tggacagtta tacttatatc caatatggtg
     2641 cttttgcctc tgctcaaact ggcaatatca ttctatcgat catccaagca tttgacggag
     2701 aatggctaag tgttggtaaa aaagtattgt ctaccctttt ctttttctta gggattcttc
     2761 taacaaagta tctaattgat tattttagaa aaaaagaaaa acatttctgg cgcttgtttg
     2821 ttctatatta tgaagcagtg atttttttct ttgttagttt gacacccgtc catgagcatc
     2881 ccgcattagt aacgattttg attgctttta ctgcatcgat ccagtgggta gcttttgata
     2941 aaatcaacgg gcgtgcctac actaatttat ttacaactgg aaatttaaaa ggggtagcaa
     3001 cgaacttata tgattattgt atttctaaag aaaaagcaga ttttgatcga tttttccact
     3061 atctaagtgt cgttcttgcc tttatcgcag gagctattgt ccttgttttc tgtcatcatc
     3121 tgtttggcgc ccattcgatt ttgatcgttt ccattttatt tttgatcatg gccctcactc
     3181 agacatttca tttatggcaa ttttatcata cgaatcggtt cactatttga tctcaatgaa
     3241 tcaaaaaaac taagcaagat gctgctcaca gagaaatatc tgcgagtgag caatttgctt
     3301 agtttttata ttatttagtt gtaaagattc cctttgcttt cgggttattt gtagtgtagc
     3361 ggatcgactg ggcatctgcc tcttctgtca ttttctttgc ccgcatcttg atttcttgta
     3421 cgatttgttc tccacatgcc ggatcggcta cgaaatcata tttgtcccca tcgatcacta
     3481 ttttgggaca tatatcaaac tgttcatacc actcttcgta tcgtcgattg agttctttat
     3541 agtaggaata gagttcagga tcttgttcta gctgttcata ttcccggcct ctttttttga
     3601 ttctcgctaa catagtttca aaggatacac gaatataaac cagtagatct ggtttctttt
     3661 gtggcgaaat ttcttctaac tccttcagca tcgtttctaa aagaccttca tactgcttta
     3721 cttctacttc agaaacacgc cctaaatctg cattcaaatg aaacagcata ctatcttcgt
     3781 agatggagcg gtctagtacg ttgtcatcat ggctgaaagc atctttgatt gccaaaaagc
     3841 gtttgttcaa aaagaaaatc tgtaataaaa aagtatactt ttctggatta gaataaaata
     3901 gcggcaatac ttcattatct tccacgttct cataaaaagg ttttgtttct aaatcttggg
     3961 caagcatttc agttagtgtg cttttccctg caccgatcgt tcctgcaaca atgatcacaa
     4021 ttatcggcat ccttcctgta ataaaatctg ttcttcattt tactacttca tttatagaaa
     4081 taaaagcctt gactttatct gctccttaaa agctacttct ctaaaagtag ccaaaagtaa
     4141 ctcctattca tgaaaataaa ggctgtgaca aaagtcttgt cgcagccctt ttttcaaata
     4201 aacagtattc tcctgccaac acctcggtca caaggcaata ttcataaaac atgaaaagct
     4261 gttttttaac attattcctt gtaactcaga gctaaacggg agactcacaa cctctttatg
     4321 ctctcaacat tttatctgtt tttattcttc tattgtactt gatttgcgta caactaactg
     4381 ccagtgcaga ttatgagcgg tatcattgtc ttctactgcg catttcatga cacgtatcat
     4441 cacactattt tcatagattt tttctatttt tccttttatt ggtgattcaa ataaatcaga
     4501 tttaaatgca tatgtttcgc cgacttcaaa caattgttca ttttccataa gaatccctcc
     4561 atcacctttt ataaggtcag taatttttta acataaatca ctcgaaaaag cacaaacttg
     4621 tcataaaaag tacttttcgt atcaaaactg tcactttttt tggtaaggtt cttggaaaac
     4681 gaagattttt ttaggagatc atgacggttt tgatgtcaaa accacttttt atgaaacaaa
     4741 agacgttttt tatttttttt agcataaaat ttgtggtgga ggtacgtatg agatgaaaga
     4801 taaagttgtg aacaaattat tagaagcctt attcagcgga gacaaatcgg aagaagatat
     4861 ggtgtatgtc caagtaaaat ttgccttaga agttttattg aataatcttg gaaaactagc
     4921 ggtcgttcta gtcttttctc tggtgacagg ctcatgggcg gaaacaggaa tcacctttct
     4981 ttcttatatc tgcatccgca gatatgctta tggattgcat tccgactctg aattcgtttg
     5041 tctgctgtgg acactacttt atttatgggg cgttcccttg gtgatgaaac atctgcaatt
     5101 gactatctcc ttcccaatga tggtcttttt gttaatcagc tgctttttgt tactgcttcg
     5161 ctatggatca agaggtacag caatcaatcc cattgaaccc gagaaacgtc ccccattatt
     5221 aaagaaggcg atcagcatgt ttctaatttt ttctctcatt actttatttt tctcagcttc
     5281 ttacttttct acctatttgc tacttgggat tgttttagaa atagctacgt tgcttcctat
     5341 tacaaactac ttgatgaatt ttggaggaat ctttcatgaa aaaaacaatc attaaacaat
     5401 taactaactt atctgacttt tttgtatctg gctgctgctt tggtatgagt tctgaattca
     5461 aaatccctga aaaattaaaa aaataatacg ctaattgtgc tataatttgg taaaaatcaa
     5521 tgggagctgt actttatgtt tttatggtcc atagcgttgc aatatttgat ttacacaatt
     5581 tgtatcttat tgattgacca tcacatccga tacaaatatc tgtttgcggg agtcattctg
     5641 atttcttgta ttttgattgg atgggtctat tcgattttag ggatttttac tgctctaatt
     5701 gctataggaa tgctgatgct tacttttttc ttcttttcga cttcgcgtca acaattggtt
     5761 tacgcgcttc cttttgggtt attgacgtcg cttttaggag atcacttaac aagcatcgac
     5821 gatttttttg ttttgaaatc ttcggttatt gaaccaggag attcattgca gtattttcac
     5881 attgtcttat ccgctttttt atctgcttta ttagcttttt gtttaaaaaa atcgttagag
     5941 acttggtttc acactgtgga ccgccgtatg atcggtacga tcgggacatt gatattattt
     6001 acatactata tcattatttt ttatacccgt ttttctggtg aaacacctga tgtcttagct
     6061 ttgaatacga gttttttcat tctatatctg tgtgttggcc tgatcatctt ggtttattat
     6121 tggaaaatct ctaataagaa attagctgat cagttattag agcaacaagt caggatgcaa
     6181 caggattata tcaaagatct agaaaaaaac tatcaagact tgagagaatt caaacatgat
     6241 taccaaaatc tgcttttttc tcttcacagc tatatttcag aaggcgactt agaaggatta
     6301 cggtcttatt atgatcaaac gatattacct acacgccaga tgatgaacct ttttccggcc
     6361 aatctaagta tgttggataa tatgaaagtt cctgagatcc gtagtttact gtcattaaaa
     6421 ctcatgatgg cacaggaaaa agggttgatc gtccagcttc attttcctga gccaatcgaa
     6481 ctggatacga aacatacggt aaaccttgta cgtatgcttg gtattatctt ggacaatgcg
     6541 attgaaggag ctagtgctgc aaagaagaaa aaaatcgaat tgaccatact caagaagaat
     6601 cattccgtga tgattcgtgt gatcaataca acaacaaata cattaccgtt aaaccagttg
     6661 aaacaaaaag ggttctcaac aaaaacaaat ccaaaaaacg aaggccttgg cttgtttatc
     6721 ttggatgaat tgacaaagac caatccttat cttttccttg aaacatcaat tgaaaaccag
     6781 cgtttttctc aaacgattta tatacaaata aaataaagga gtaatgatat gattcccatt
     6841 tatatttgtg aagacgatcc aaaacaatta gaagttttat caacggtgat caaaaataga
     6901 atcatgattg aaaacttaga cagttatgtc catatttcta cctctgatcc ggaagaactt
     6961 ctcaatgcag ccaagatcag aacttcttct tttggtattt actttttaga tatcgaacta
     7021 gaagacagtg aaatggatgg tgtagcgatc ggtaaattga tccgtgaatt agatccttta
     7081 ggaaaaataa tctatgtgac ttcccatacg gaaatgtcga tgaaaatctt aaaaagcaac
     7141 atcgaaccga ctgattatat cgtcaaagaa gatctttatg atttaaaaga aaatgtcgag
     7201 catatcctga gcaaaatttt cgaagatttt caaatgtccc aaatccaaaa agatatcttc
     7261 aaaatagagt ttaatgatga gatcaaattt ttaccgatca aagacatcca atatttttct
     7321 acttcgctag gagcgccaca taaactggaa gtccatttga ctcatgccca attacaattc
     7381 tatgagaaga tcaaagaaat cgagaaaatg catcggaatt ttatccgttg tcataaatcc
     7441 tatgttatca atacacaaaa tgtgcggtcg attaataaaa aaaccagaga agtcacgttg
     7501 gcaaacggcg agaccatccc agcctctatc cgtggattaa agaaattgat ttcataaaaa
     7561 gaaccgaata gtttggaccg actaaatgac cagccgatcc aaacccttcg gttctttttt
     7621 gcctatcagt gcaaaagcat ttcctgtgca atttcacgtc catccattgt atctggataa
     7681 aatgttggcc aattctccat ttctttcagc aatacttctt gactttcatt tcctgcatat
     7741 aaatgaaaat gttcggagtc agccggtgca atcgtgtgat cactgaattg aatatatcta
     7801 aaagcgccac tttccttatc ttctgcttcg aatagatacc ttacgccacg gtttcctgcc
     7861 tcgtaggtca agatttcttt ccctacataa cgataatttg aacgtttcgt tgttccatcc
     7921 ttaaacgtaa atgccatctc tttctcactg atcttgattg aagcaatatc tgtttgatag
     7981 cccttcgtat aatattcttt atattcttgt gctgtcttat cctgattcaa tttttcttta
     8041 tattcaaaaa caggatcaaa agtcccatct tctacatacg tatctactgt ttgccaagtt
     8101 cctgcccaat ctgaaagaga gcgatcttgt acatcctcgt cttcaaaata accgttgtag
     8161 acgctctttt cttttttggg ggtcaaagta tcttctagcg ggttttctgt ctctgtcgtt
     8221 tttttcaacg cttcgaggtt tgcttccatg atagaaagat agttttctcc attttctatt
     8281 tgttcattcg tcaatccttc caaaggattc aatacttcta aagatacacc tgcttcgttt
     8341 gctaacgttc tggcaatact atctttggcg ttttcttcaa aataaatata gttgattcca
     8401 tgttctttaa caaagtgttt cagttcagca attctcgccg cagacggttc ttcactagaa
     8461 gaaagaccag caatcgatac ttgattcaat ccatattcta acgctagata gttgaatgca
     8521 gcatgttgcg tgacaaaaga tctttgtttt gcttgcttaa aggtttcttg ataaagctga
     8581 tggagtgctt gaagtttctc ggtataggct gctgcatttt ttgtccatac ttcctgtttt
     8641 tctgggtaag cttcaatcag ctgatcacgt atgtttgcta cctgcttgat tgctaaactt
     8701 ggagctagcc atacatgtgg gtctagctca tgtgtatggc cttcttcccc gtgttcatgg
     8761 tcatgatcat gtccttcttc acttcccgga agtaaaagca taccttctgt cgcttttatc
     8821 gtatggacat tgccttcttg taatgttttt tggattgcgg ggacccatgt ttccatgttt
     8881 tcatcattat aaacaaagat atctgtatct tggatctttg ccatgtcttt agcagaaggt
     8941 tcataatcgt gggaatcagt ccctgctgga atcaataact ctacttctcc ttcatccccg
     9001 acgattgctt ttgtaaaatc atacatggga taaaaggtcg tcatcacgct caatttttct
     9061 ttactgtcct cggctttccc acttgccccg caagcagtta acatacctgc taaaaccagc
     9121 atggccaata tacctatcaa tcgtttcatt tatgacactc ctcttctcat cttctttcat
     9181 ttgaatgttc cgattcgctt ttcgcaaacc gtaatagtta cgttttagca acaggattta
     9241 atttatcata tggttaaaag aactgtcaag cgttttctta ttttaattga tgagaaagtt
     9301 tatatgaatg attgacgata tttttgcaat caacctctat cattttcctt ttatacccgc
     9361 ttttgtctat aatgaagtta tagataaagg aggaaaaaga gtgaaaaaag aaacagatac
     9421 aacgaatcgt ttgatccgct ttttaggagg aaaaacctct tactatgttt taggaatagt
     9481 tatcttactt gcgctgatca tttatttttt ccatcagatc tcgtttatat tcaagcctgt
     9541 cggaatcatt atcgctacta tcttgccccc attattgttt gcactgatct tgtattatct
     9601 tttggaacca gtcatcaaat gtttgagcaa gcacctttcg aggaacttgt cagtcattat
     9661 cgtttatgtg attattttat ttttattggg gcttggaagc atatggctta ttccttttct
     9721 tgaagaacag gcaaagacat tgatcagtag tttgccagat ttgtttcaag attttcaaga
     9781 gagtctccga caatttttgg agaaaacacc ttttgccgat tcatttaatc aattttttgc
     9841 ttctattgac gacgtgacgt ctgatatagc tggatttatt ggaaattatt gggaaagtgg
     9901 cgcacaacgg atcagcaaca ttttttcaac tgttacagct atttttatta ctttatttac
     9961 tggtccaatt gtcgcttttt tccttttgag agatcctcaa aaattttatc agtcagtttt
    10021 agcgattgtc ccaccagcat ttcgtacaga ttttaaaaat ttgacaaaga ttgctaatca
    10081 acaattgggt tcatttctaa aggggcaaat cattgcttca tttatccttg gagcagtcta
    10141 ctgggtttgt ttcttactga tcggattaga atttgctagt gttttagcta tttctgctgg
    10201 gttgttatgt atcattccat atattgggcc gtttatcgcc ttctttccag gtttgatcat
    10261 tgcattccaa gactctactt ttatggctgt caaatttgtc attgtctggt ttgccgtaca
    10321 acttctccac ggtgacttgg tgattcctcg agtaatgggt gatcgtttac agatccatcc
    10381 gattacgatc ttaatcgttt tacttgtcat gggtgatcta atgggaattg tgggcgttat
    10441 ttttggtatt cctatctata cgttagtcaa attactcgtt acttttattt tccgtaaatt
    10501 caaacaacgc tacaataaat tttatggcga taaaggagag tatgaaaact cttcattttc
    10561 tgaagaagat tattttaaat aaaacacaaa acgtcgatct tacgagaata gctcgtagga
    10621 acgacgtttt gtgtttttgt tttaattttt cagcaaagca attgcctggt atggttttag
    10681 ctccagactt tgatgaagaa cagtatcgga ataattatcg ataagaatat tggcttctgt
    10741 gaatccttct gggatggcga tccttgtatc tttatcatag aaatttgtta atacaagcag
    10801 ctgctgtcct tcaaattggc gaagaaagcc ataaacttcc ggatgatttg cttcataagc
    10861 atgataatct ccttgtgcaa cgattggata tgtttttctc agccgaatca atttcttgta
    10921 ataggacaaa atagaatctt cagaagatag ttctttttct acattaatct cttctagctt
    10981 gcctccaact tttaaccaag gtttccctgt agtaaatcct gcttgttctg tgtctgacca
    11041 ttgcatgggt gttcgagagt tatcgcgaga ttttgctttg atccgacgaa acgcttcttt
    11101 ttctgtgaat ccttgttcaa ggagtaactt ataagcattc aaactttcta cgtcaatata
    11161 gtcggaaatc gatgaatagt ctgggtcgat catgccgatt tcttctccca tataaatata
    11221 gggcgtccca cggttcaaat ggatcatggt tgctagcatc tctgctcctt gttttcgata
    11281 ctgatcgata gctacaaatc gattgagcgc acgtggctga tcatgattat taaaaaatag
    11341 agcattccag ccgttaccct cactcatctt ttctccccat gtatgaaata gtcgctttaa
    11401 ttctgcaaaa tcaaatggag gagtagacca tttattccca ttttcataat cgactttcaa
    11461 atgatggaaa ttaaagacca ttgataattc ttctctacta ggttctgtat acaagataca
    11521 attttcgatc gtcgtagagc tcatttcacc gactgtgatg atagtggaat ccgtcccaaa
    11581 cgttgcttgg ttcaattctt ttagatacgt atgggtgatt ggtttgtccg tatattcagg
    11641 tttgccatca ttttctttat tatttttcaa ttgttcgtct ttaccgataa cattgataac
    11701 atcgaaacgg aatcctttta cccctttttc catccaaaaa cgaacaactt tgaatagttc
    11761 ctctcttact gcttggttcc gccagttcaa gtcagcttgt gtccgatcat ataagtgcaa
    11821 ataatatttc cccgtatctc caaatggcga ccaagcactt ccaccaaatt ttgataccca
    11881 atccgttggc tcttctctta aaatgaagaa gtcctgataa tgctggtccc ctgctagtgc
    11941 ttttttgaac cattcatgtt ctgtcgaaac gtgattcagc accatatcga gcatgatttc
    12001 tatccggtat ttctttgctt cggctattag ctgttcgaat tcctccatcg taccaaagat
    12061 aggatcgatg gctgtgtaat cagagatatc ataaccgttg tctttttgag ggcttggata
    12121 aatagggttt agccaaatca tgtcgatgcc tagatcattc agatacggga gtttttcttt
    12181 gattcctttt aaatctccaa ttccatcacc attactgtct ttgtaagatt tgggatagat
    12241 ttggtaaatg actttgtctt taaatgtcat ggatatttac ctccattcaa ctgatttatt
    12301 acctaccaca acttatatat acaagttatc atttattatt ataactgaac cgaaagcgtt
    12361 tgtaaaattt atctttatac attctaagag gtctgttgcc caataaaaaa caagagatca
    12421 gacatgtatt tgtctatcct cttgtttaaa atagcaaaat attcttgttt cttctataaa
    12481 tggtgttcaa aagcttttat ctacggcaat cagtcttctc aaatgtaaaa catgaggagc
    12541 taattttatt tgagtattct ttttgctcga tgcaggacgg cttttttaca acctctgatt
    12601 catggtgttc aattagcagc tgaccctcgt tattagctca aaacgctgat ttcacaacct
    12661 ctgtttttat tcattcgatt tcaacgcatc aatcatatcg atatgcttta atttgaaatg
    12721 ggtaacaatc atcacaataa ccgtaaatac gatagttaat cctgctgata agacataagc
    12781 ccaccatgtg atcactaatg gaaagatgac attctccata gacgcttgtt gcaagatgaa
    12841 gtcagtcaat atatatccta caccaaatcc tccgatgatt cctaaaagag taaagatgat
    12901 attttctcga acaatgtaca tcgtgacttc tttatcaaaa aatccaagga cttttattgt
    12961 agataattcg cgtacacgct cggaaatatt gatattagtt agattataca agacgataaa
    13021 agccagcagt cctgataata cgacaaagat taagacaacc gagtctaagt tcgccattga
    13081 ttcttcttgc gtttcgattt gtttggacat gaaagacgta tttgtcacag cgtctgtggc
    13141 aagcagtttc tcggaaagca tttcttcttc ttttgttgtc atcttatctg tacgcagcaa
    13201 aaatgcatta tccatcatcg attgatcagt aactgaagca taatattcag gggacatata
    13261 aataaagcta cctagatagt ttttcacgat ttcttttact tcgactttcc attctttttg
    13321 atcttcatca taaagcgaaa tatgatctcc tgtagataag tcaaatagtt ctgctacttt
    13381 ttgcgtaata gcgattcctg ttttaggcaa ggagcgtggg ttctcttcaa gatcagttaa
    13441 atggataaaa cgatccaaat cagctgtttt acttggtacg atcactgtca gactttgcga
    13501 agcatgtcct ttttttctca attctacggt ttgagtatga atagctagga tctcatttac
    13561 tttttcttgc ttctgcagca tatccgctgc tttttgacga ctatcttccg tggatgaatc
    13621 caatgtaacg attgcttgat agtcaatgat cggtccaaat tgtttggctg aaacagaact
    13681 tagagaatct ttcaatccca cgcctgcaac catcagcccg gcacatccag caattccgat
    13741 aatcgccata agcattcttg acttatatcg gaagagattt cggtaactga ttttttgatt
    13801 aaagctaagg cgtgaccaca gcggagtgat gtattcaaga aaaatgcgtt tccctggctt
    13861 cggtgctttc ggttgaagca atgcagcagg ccgttctctt aaatctttga ataaaacgat
    13921 cattgcagcg ccaaatgcgg caatgaagaa tgcggcagct gcttgtaaaa tcggcggcca
    13981 gtcataaaaa ataagcgcac tcccaatatc ataccgctcg ttagacagct caaaaatgat
    14041 ccttggcaaa agattcgttc ctaatatcgt tcctaaaaca atacctatcc ctgaggctag
    14101 acttgcatag attgcatatt tttttgcgat ttcaactttc gtataaccta acgcctttaa
    14161 tgtcccgatt tccctgcggt tttcttctac catacgtgtc attgtcgtaa aggtgatcaa
    14221 tgcggcaata aagaaaaaga aaacaggaaa aacattggca atcgcagcga tacgatcaga
    14281 cagatcacca aattcttgaa aaccgacatt ttctgaacgt tcattgtata ggtatgatgg
    14341 tttagttaaa ttttcaattt tttctgcatt tcgattcaat tcttcctctg cagaggccag
    14401 ttcacgttct ttctcaacta attgcctttc ttgttcagca agttgagctt cttgttgaga
    14461 taatgtttga acagcgattg ttcgttgcgc ttcaggaagc tgctggatag ttgctgactg
    14521 ttgcgctaac tgttttttag catcttctaa ccgtgttctt gcttgcgttg tttgtgcttt
    14581 gccatccgct attttttgct gtgccggttc taattcttcg ttggccgatt ttttcagctc
    14641 ttctaagcgt tcttcgggtc gatcttgtaa atacttttct gccttttctt gattttgctc
    14701 cattttatct ttgtaagctt tgctgtacgt attcaaattc tttacgtttg taaaatcaag
    14761 atataagaca gaataaatat ctgttttaaa aacggattca ggtaaataga caaaataatc
    14821 gacgctacca tttccgacgt tcgcgtatcc tctctccaaa ttgttgatga acaatggaga
    14881 acggacaaag cccgtgatgg tatactcttt ttctttcaat tcatctgaat caatctgata
    14941 aatatcaccg attttatacc cttcatcttc cgctttttca tccaatacaa gctggtttgg
    15001 tttttccggg agttttccat cagttaattg tagcttgttc tgcttgttat ttttgttata
    15061 agaaaatatt tgaatcactt cgtttttgcc agtattggaa taatagatct gttttcctgt
    15121 ttctactttt gcatctggga tcttttctgc ttgagcaata tctttttgtg tcaagccacc
    15181 agtagaaacg atttgcaaat cagaaagtcc tgctgtacca acatagtgat ccatcgtttt
    15241 ttgcatgaca ggtccagcag ttttgacacc tacaaataaa agtgttccta gcatgacaat
    15301 aaggatgatt gcaatgaatc gtcctttaga ttgccgaatt tcgcgaaaac ctgctttaag
    15361 atatgttctt gttttcataa aatcccccta ccatacaatc tcatcaatgg atgaaggatg
    15421 atcatttaat tcaacagacc ttactttagc atcattaatg tgaatgactc tatcagcgat
    15481 gggcgctaaa gcagaattat gtgtaatgat caaaacggta ttgccgtgtt gtctgctagc
    15541 gttttgcaat aatttcaata cttgcttgcc cgtttcaaaa tccagcgcgc ctgtcggttc
    15601 atcacataaa agaagtttgg gatttttagc tagtgcacga gcaattgaaa ccctctgctg
    15661 ttcgcctcct gataattggg acggaaagtt attcaatcga tgagctaggc ccacttgcct
    15721 caatacctct acaggatcaa gcgcatcagg agaaacttcc gttgccaatt ccacattttc
    15781 ttttgctgtt agattaggaa cgagattgta gaattggaaa acgaacccta catctgttcg
    15841 tcggtaagca gtaagctgtt tatctgaaaa ttgcgcaata tctgtgtcat caataatgat
    15901 ctgaccttca tcaggtgtat ccatcccacc taaaatatta agaatcgttg actttcctgc
    15961 tccacttgga ccaagtataa tcactaattc gcctttttca attgaaaaag aaatatcgtc
    16021 atttgctgta atggtcgttt ctcccatttt gtatcgtttg tattcatttt ttacttcgac
    16081 atacttcact ctgattcctc cattttcacg atatttctcg ctatatctat gcatcgaatg
    16141 cgttttctca cacttattta ttttaccact gaatttctct agactaaaga aaggaatcct
    16201 ttaatttagt tgacaaataa acgatttatg atttacttac ataaaaaaca ttgttatttg
    16261 aaagaaggaa catcatgaat gatttaaagg aaaatatgat ttttgattta tcaaaaactg
    16321 cttttgtcgc gatcgacatg caaaaagaga tcgtaaataa cggtacactc tctccacaca
    16381 ctgcagcatc tattctgaca aacaatgatt tgttagtaag aacattgaaa cgtaccgctg
    16441 ccttgaaagt tttagtgaac gtagacatct ccactttccc ttatttgagt caacaaactg
    16501 atatgggtgg aatgactgct tctgtacctc cagaattcac tgatttactt ttgaaggatt
    16561 ctttgaaaga tacagataat atgttgacta tcacaaaata caatccaagt gctttttttg
    16621 gtacttctct tgatcttcaa cttcgccggc gtggaatcga aactatcatt ttaagcggtg
    16681 tagcgacaac aaacggtgta tatgctactg cattagatgc ctttcaacac gggtaccata
    16741 tcgtgcttgc tgaagatgct tgcagtgatc gtgacaaaga atcccatcaa ttgttcataa
    16801 aaaagatatt tccgaaaact gcacgagtac gctcaacgaa acaaataatc gaagctatcc
    16861 aacaaagcaa ataagtactg gcacacgtga ttttcattta cttctttttg atttcatggg
    16921 aaaataacca caggatttag ataggagtat gaacatggtg aaaaaaaaga tcacaatcaa
    16981 agaagtcgct aaactttccg gcgtttcaat cacaactgtc tccttgatat taaatggaaa
    17041 tacggagaaa ttcagtccga aaactgttca gaaagtttta gctgccaaag aagaattagg
    17101 ctatcagcct gactattttg ctagacaaat gattaccaag gaaacgaaaa caattggtgt
    17161 actcgttcct gatatcacga atccattctt cagtacactg atgcgaggaa ttgaggatat
    17221 cttgtataag cagaatttcg tcacgatatt atgtaatgct gattctgatc atcaaaaaga
    17281 aatcgaatat ctagccgagc tcacacgtcg aggtgtggat ggattcatta tcgcgacttc
    17341 tgctgtctct acagatgcaa tcaacgaaaa tttgaaaaaa caaggccggc ccttcattgt
    17401 tttggatcag aaaaaatctg aaggatttag tgatgccgta cgaacagacg attttcgcgg
    17461 tggttaccta gcgggcatgc acttgcttag tctaggacat caaacgatcg cccttgtcta
    17521 tcctgaaaat ccaccggaaa atgtccacgc tcggattgag gggtttaaat ctgctcttga
    17581 tgtttaccaa atccctcacg accaactgat cctcttacct acacagttct caaaacaagg
    17641 tggctaccag atcacagcag aacttcttga ttctgctgca actggtgtgt ttgctttaaa
    17701 tgatgaatta gcttttggat tatatcgcgg attagaggaa gctggaaaaa gcatccctga
    17761 agactacagc attattggct atgataatat agatatgtgc gagtatatca agccgaaact
    17821 gacaacgatc gcacaaccaa tcttcgaatt gggacaaaca tctgccaagt tattgttaga
    17881 tcggatccaa ttcccagaaa aagaatggga agaaaaaaga ttacctgtac gattagaaaa
    17941 aagattttca actgcaccct tgaaatagct ttcaaggtgt gctatatttt tatcgaacta
    18001 ttaaaacgtt ttactaaaac gttttaaaaa ataattttcc catcaaaata aatgagatag
    18061 ttggaggttt gtaaaatgaa cacggttaca gtaattggaa gtattaatat ggacactacc
    18121 cttcgtttaa ccaacatgcc caagccaggc gaaacgatgc atgcgcatga gatttttcac
    18181 gctggcggag gtaaaggtgc caaccaagca gtggcagcca aacgttcagg cgcacggaca
    18241 tcttttattg gaggcgttgg ggctgacagc gaaggacaac agttattaga tctactgaca
    18301 aaagaaaaca tcgatacatc tgggatagct gaaatccaag gcgccacaac cggacaagca
    18361 atgattatgg tagacgcagc aggagaaaat agtatcttga tccatgcagg ggcaaataat
    18421 gctttccatg aacaagaagt cctaaaaaac aagcaactga ttacaaacag tgactttatt
    18481 attgctcagt ttgaaagtag cttagatgcg acgatcctag ctttttctat tgcaaaagac
    18541 gcaggaaaaa caacgatcct gaatcctgca ccagctcgtg aaacgattcc tactgaacta
    18601 cttgaaaaga cagatatcat tataccaaat gaaacagaaa cagaaatcat tacaggaatc
    18661 agagtgacag atcataacag tttggtcgca gcagccgaaa aactccatga attaggtatt
    18721 ggaacagtaa tcattacact aggcagtgcc ggcgctttct atcatacaga aaaagaacac
    18781 ggtatcgtcc ctgcttttaa agtggatgcc gttgacacaa cagccgcagg tgatacattt
    18841 attggcgcat taagcagcac acttcagcca gaccttagta acttgaaaga agcgatcctt
    18901 tatggaaatc tagcctcttc tgttgctgtg cagtcttatg gtgcgcaacc ttctatccct
    18961 taccgcgaag ctttagaaca atcagcagtc taaaattaac aatgagagga agaaacaaac
    19021 atgaaaaaag gaaaagtaat caattcagat atttcacgag taatcgcaca aatggggcac
    19081 tttgataaat taagtatcgg ggatgcaggc atgcctgtcc cctttggtac tgaaaaaata
    19141 gatttggctg tctcaaacgg tcttccaagt tttttatctg ttttagaaaa cgttttagaa
    19201 gaacttgagg tacaatcgat tgaattggcg gaagaaataa agacaatgaa cccggaaata
    19261 ttggaaaaaa tcaaattatt gttaccagat acaccgatta gctttatccc acatgaagaa
    19321 atgaaaaaag agttgaataa ctgtcatgca tttattcgta caggcgaaat gacaccttac
    19381 gccaatatta tattagtgag tggagtggtc ttctaatgga aacgacaatg aatcctgaac
    19441 agatggtact gtccgagcag attcatatgc tgattgaaca gttgggagga aaagacaata
    19501 ttgattctgt ccttccatgt gcgactcggc ttcgtatcaa attgaaaaaa cagccagtag
    19561 ccaatgcaga aaaattaaaa cagcaaatcc cttccattaa gtatttcatt ttactcgata
    19621 gatatgcaga aataatcatg gatcagggat tggctcctgc ttacaaggaa ttgacgcgtc
    19681 aaatcgatca ttcagctccg aaagaaaagc taaatccgat cgaaaaacta tttggtttga
    19741 tatcggaaat ctttacgccg atcattccgc tgtttgccgg ttctggtata ttaaaaggtc
    19801 tggttgtttt agcgactacg attggctggc ttagtgaaac aagcggaact tatcatattt
    19861 tgtctgctgc gtcgaatgcc gtcttttatt ttctgccaat ctttcttgct ttttctgctg
    19921 ccaaaacgtt taaagtgaat ggatatattg cggctgtcat tgctgcagca ctgattgaac
    19981 cgaatctgac ctctttactt tcagacagtg gcaaagcagc tactgatttt ctggggattc
    20041 ctgtagtact gatgcagtat acgtctactg tcatgccggc gattttaggt attttctttt
    20101 attcttttgt tgaaaaattc ctcaaaaaat atattcgcga aaatatgcaa ttagtatttg
    20161 tacctttact ttcattagtt atcacggttc ctttgacgtt aatggtattc gggcctgcgg
    20221 gtgtttacct tggcgaaggt ttagctgccg caattacttg gatgatggat attaacggac
    20281 cgatcagtgg cgcaatcatc gctggtggtt ggaatatttt ggtaatattt ggtatccaat
    20341 gggctgtaaa ccctgtcatg atcagtaata tctctgctta tgggttcgac cgaatcgttc
    20401 cgttgacagg agcagccaat tttggaatgg ctggtgcagc tttaggtgta tttctgcgaa
    20461 gtaaacgttc aaaaacccgt tcgatctccg gttctgcttt tgcttcgatt ttacttgcgg
    20521 gagtgaccga accaactgtt tacggaattg cgatcccatt gaaaaaacca tttgttgctg
    20581 cttgcattgg tgcagctgct ggtggagctg taatgggatt tgcccaagtg aaagcgatcg
    20641 cttttgtatt tggtagtctg accacattgc ctgcattcat ttccggtact tttttctggt
    20701 acttagcagg actggctgtt agtctagtcg tagcaatgat cactactctc gttagcgggt
    20761 ttgatgaaga cctaatgtct tatgaatagt taatgaaaaa gactgaagta tgagatgttt
    20821 atcacatatt tcagtctttt tctttttagg atcattattt ttataaattt ttgtgatact
    20881 ttccaaactt tggtataatt tacatatgca taaccatttt tatccccact tgtttatccc
    20941 caaacaaagg tggtctatgc aataaaatga acgtcaaaga aaaacgaatg aatgctcgtt
    21001 gcttatttac aaattgtttt atttagaata cacagacaga tttatgaaaa gaaaaagctt
    21061 ttcgacacga ttatctgttc caaaaaatgt ctaatgaatg ctgaataaga cagaaaatca
    21121 atgaaaggaa aaggactagt ttattatgaa aaataaaaaa acgatacgtt tgctagctgc
    21181 tacttggctg attgctccgt ctgttttgtc gaccaatacc gtatttgccg tagataatca
    21241 aatagctgat caaacagtca caacagatac gactacatcc agctcagcta ctagtgaaac
    21301 aacggattct tttacagaag acccaactgg aacaactaac tcaacaacag gatcgaccga
    21361 gccaactgaa attgatccgg ctgatccatc agaaaatgaa aagccagccg agacatattc
    21421 ccttacaatt gctccttctt tgattggtaa gatccaactg actgcttcga cagcggattc
    21481 ccctgaacag caacctttgg atattacgga aagtggaacg gtagaaggtt tattagcgga
    21541 aacgaaggta acttacacta ttacgccagc tgaaggtgaa caaattgctt cagtcgctgt
    21601 taatggtgcc aaagaagtaa attatgatgc aaagaccttt acagtaggta cgaacaattt
    21661 atctattact gcagaattta aagctggacc ggatacttca acagagaaac caactgatcc
    21721 tagtgaagaa gtccctagta caccagatcc ggaaacgcca aacaaaccag atgtgggtaa
    21781 taacaacaat aataatggtt caaataattc aaacaataat aacaataatt cgaacagcgg
    21841 gaattcttcg aatcaaacaa acccatctgg atcgaataac aatggaaata ctcagagaga
    21901 accaaaccat ccaaacaatg gttcatcgaa tggcggaacg aacaatgctt catctcgtcc
    21961 aaatacagca aatcaaagcg gtatagagaa tccatcttct gcttcttctg attttgttgt
    22021 aaaatcacca attgatgctg tattgcctac aaatacaacg aatgtccaac aagcaatcgt
    22081 tcgagaagca ttcaagcatc ttggaaaacc atatgtatgg ggagcaaaag gtccaaatac
    22141 ttttgactgt tctggtttga cttattacgt atatatgaaa gcaaccggtc attatatcgg
    22201 tggctggaca ggcgaacagc aatatgcagg tacgcagatt ccagtaagtc aggcccaacc
    22261 tggcgatctc gtcttctggg gaccttcttc cggtgttact caccatgtag gtatctatat
    22321 tgggaacgga caatttatcc atgcgcctca accaggagat aaagttcgag taacttctat
    22381 ttcagatttc acgcctgatt ttgctgtccg agtaaactta gcaggtttac ctgctgcttc
    22441 aggttcgctg acaggcggct catctatttt agatggatta gatggctcgt tccatttcag
    22501 tcaaaaccaa acaactgatc agttcttgaa aaagattgca gatgatgcac aggaaatcgg
    22561 gcaaaaagaa ggaatctacg catcagtcat gatggctcaa gctatccttg aaagtggatc
    22621 tggaaacagc ttattatcaa gcgaaccaaa tcataacctt tttggtatca aaggatcata
    22681 taaaggaagc agcgtgacct tcaatacact tgaacaagat agttccggac aaagctacca
    22741 aatccgcgca caattccgta aatatccttc ttacaaggaa agtttggaag actatgccga
    22801 cttgatcaaa aatggactga ctggcaatcc tgatttctac aaaccaactt ggaagtcgga
    22861 aacaaaagac tacaaagaag caaccaaata tttggaagga cgttatgcta cagatcgcca
    22921 atattcacaa aaattgaacg cgatcatcga agcgtacgac ctgacaaaat atgatgaacc
    22981 gaaaaaagaa gcagcaacaa ctgaagaagc aaacgctgaa atcctcaata tgtctaagcg
    23041 attcgatgtg ccaatcacag gattagaatt ttcaacgata aaacgcgtca acaacgcttc
    23101 ttccatcttc cgtgtcgctt caatctggga tttgtggaac aattttacgg caagacagat
    23161 accagaaaca actactcgta cagttaaagc tgcaactgtt cctgtactaa ccttgttaag
    23221 tttagacaaa tcattctggc ttgatctgcc tttaaagaaa tagaataaat taaaaagggg
    23281 atgtgacact gtcacactcc ctttttaatt tattctattt ctttatattt attttgtatt
    23341 ccgttacaaa ctcgcgtact tggttaggtt ccagagtgac atctccaaaa ctttcaagtc
    23401 gctccgctcc tggcagttct tgagtctcaa atgtgatacc gccatgattt actaatttat
    23461 tcccgcgcat ttccggtcca tcttctccaa aatttgctgt aaaaatcacg attcctgaag
    23521 catctgtttt tacttggatc gatacttttt catctgggct tgtcaattcc gcagcattta
    23581 gtaaaccaga ttgttttttc aacaaaaatg gatgatcgat gccgcccact atcattgatt
    23641 ggcgaaaatc actggcaaag acctcaccta attttttagg acgttggaag tcaaatgctg
    23701 tgccacttac tgaaactact tctcctgtag gcgttgtatc tttattgagc gtagcaaata
    23761 atcggctatt caaccaaagt atatgttcgt cgattgactg tgctggatca ccggttaaat
    23821 tgaaatagac atgatttgta ggattgaata ctgttttgcg gtcactgatc ccttggaccg
    23881 tcactcgcca tgtatcatcg ttcgtcagcg tataccgtac ttctacatct aaagttccag
    23941 gaaatccgtt ttccatatct gggctagtct gatagaaaat gaccgatgct tcgttttcac
    24001 cattgatgac tgtataatgc cactgcttgg cttcaaagct aggtgcacca ccatgtaaac
    24061 aatgaccagt gtccgggtct gtttttactt cgaacgtttc atcatctaat ttgaatgtgc
    24121 cattcgtaat ccgtccagcc atacgtccga ttgaagcacc gatataaggg tctttttcta
    24181 gatattcttt tgcttcatca aaacctaaga tcaactctcg tttttcatta tcgattattg
    24241 tttgaaaatg aacaacccgt gccccaagat cactgacaga caaagatgtc ccattttgat
    24301 tcgtgatcgt aatcaagtga tagttttcgc caaaattttg ttcattgatt tccatgttcc
    24361 ttgcaccctc ttttctttca tttgcactga ttccagtata catggaaacc gttttcttaa
    24421 cagcttattc cgtgagtaac gagaaaaaat ttactatatt ctaataaaag accgtgaaaa
    24481 aaaggttgtg acaaaaatat tgtcacaacc tctttttccg aacaaacggt gttcaaaagc
    24541 ttgcatctgc ggtaataagc ccaaccaaac gaaaaatatg aggagctatt tttgcttggc
    24601 tgttcttatt acttgatgca ggacagcttt ttcacaatct ctttttcaat acgattccag
    24661 tatataacca tgtcgtcagt acatagtaga tgaaatacaa tacgaagaag atcgtaaacg
    24721 gaacccaaat atctttgtat ggattttgca tgaatagacc aaacatttgt aatccgaata
    24781 agacatggac tacacctaag attcctggtg ctaagaataa cacgcctact tctctacgga
    24841 tcgattgttt aagcaacccg cgtcgtgttc ctatcttttc aagcatttca taacgagcaa
    24901 tgtcactctt gcttcctgac aagatcttga acatcaaaca gctagcaagc atggtcaaga
    24961 aagctaaacc taggaagaat cccataaatt caaaaccaga gaatagacca ttatacactt
    25021 catacatttc atatttctgg ttgctgtact ctactttttg caaagatgga ttgttcattt
    25081 tattttgagc aacaagtgtt tcgattggtt tcaggtttgc ttggaagtct tttacctgaa
    25141 ctagctgtaa agtggattgt ggcaagttca gttgatcaaa ttctgattga ctgactacct
    25201 tgatttcttt ttcttgctgt tccggcaatt catagcttcg aagttcctct tgtacagtca
    25261 gttcattcat ttttttagca ggaacttttt ctttcgtgat cttcggcggt gttccatctt
    25321 catgttttat catgatcaat ggttgagccg caaattcact tgcattatag taaatcgttc
    25381 ccgcatcttc tttttgagta taagaagcag aaagtgttgg tgaaagttct ttgatttttt
    25441 gctgatcttc tgcccgagga ttattcatca ctagatcata tccagaattg ttttctgtca
    25501 agtctgcaat ttcattttta aaacctaatc ctaccgtcaa agcgcccagt gccaaggcaa
    25561 acagcatagc gaccattgag agcatttgtg tatattcacg aatacgaaag tttaactgtg
    25621 acaatgtgaa attgttcaat tttttcaatg aaatctgttc tgatttcttt agtaatgata
    25681 aaaagaaaat gatcacagaa tggaaaatga aataagttcc taatacgatc gtaactaagg
    25741 ctacccctat accaaagact tgataggtcc ccacgtgttt catcatatag taaccgcagc
    25801 ctagaaaagc aattcccagt actacttcaa ggaaaaacaa gaatttatgc tgcttgattc
    25861 ttgttggtgt ctgatccgca cgaatcaatg ctaaaatcgg ttttttgaca atcgaaaaag
    25921 cattgacaac agcagctaat aaaaacaaaa cagcaaaaaa caaaacagtg atcaacaacc
    25981 cgtgaatact aaaaggtgta aaatgcgaaa tattgagatc taactgatcc actaataatc
    26041 ggtggacgat acttgtcaat cctacaccga cagctgaacc cactaaagta gctgcgatcc
    26101 ctactaagaa tgtttcagaa aaaatcatct gtgcgatttt tctgcctttt gctcccagca
    26161 tcataaacat tgcatagtct ttttgccgca tcgtcatcaa aaaggaattt gcatataaaa
    26221 tataaacgaa tgtgatgatc cctagtaaaa cagaaccgaa tcggaaaatg ataactgtag
    26281 ccgcaacgat cgtattactt ttcaaaaaag cttcgttgct tgccattgat tcaaacatgt
    26341 aaaaaatagc agatgccata actaatcctg agaataacac caagtagtct cttaaacggc
    26401 tcttgatccc agtaagtgat aatttccaaa gcatagtcaa tctcccccta ctgttccaag
    26461 tttccaagaa tagatagtat ttctcgatag aaggaatcac ggctttgatc tccacgtttc
    26521 acttcttgat ggataccgcc atccttgatg aataaaatac gttggcaata acttgcagaa
    26581 aacggatcat gcgtcacaag aagaatcgat acatgatctt ttgtattcaa ttcgtccatc
    26641 gtatctaaaa gatcccgggc acttttggaa tccagcgcac ctgtaggctc atcccctaat
    26701 agaatcgttg gttgtgtgat caatgcacgt gcggcggcga cccgttgttt ttgtccaccg
    26761 gaaatctcag aaggatagct ttccaaaata tgagagatgg ataaacgttc tgcaattttt
    26821 tggacacgtt gcttgatttc ttttggtctg acgccttgta aagaaagagg aacagcgata
    26881 ttttcttgtg ctgtcaagct ttctaataaa ttgaaatctt ggaaaataaa gccaatctca
    26941 ttcgcacgaa aatctgctag ttgatttcct tttaatgtgg tcacatcttt ttgattgatt
    27001 cggatatgcc catctgttgg tttatctaat gtggataaaa tattcaaaag tgtcgactta
    27061 ccagaaccag aagcgcccat gatcccaata aattcgcctt tttctacatc gaagctgatg
    27121 ccgcttaatg cttgcgtctt tttctcattg ttttttccgt atacttttgt tacattctgt
    27181 acttcgacta ttttgttcat ttctttccct accctttaat tagtttttcc agtgctgttt
    27241 tcggcacgtc ttttttttcg acttcttcat acgaagtgac acctttattt ttgttccagg
    27301 tgatttttaa atatgctcct ttacgaagcg gtcgagtcaa ttgtcccttg aagtccagtt
    27361 ctttttcctt accctctttc gtatagccag gcaggatata tctatagttg actgtttctt
    27421 gtccagagtc tgattttgaa actgtttttg taccatctgt cgtaatttgt acatagtatt
    27481 cttctccacc cataactacg taatctgcga ttttcacccc tgcaagtacc acaacgatga
    27541 aaacaagtag accgatcaga agttttttca ctttttcccc ttctctctta ctttatatac
    27601 tgattataga aaaaagcgaa caattctgcc ttcgaatcaa ctatcattct tctttcaatt
    27661 ttgtcatttt ctttaaaaca actaaaaaaa gagttcaaga caataatccg tcctaaactc
    27721 cttcttccta tcttacaaaa agtatgtcac ttacggtctt tcatgtcgcc tgtattgata
    27781 acaggttgcg ttcctttgtc tgtaatgatt gagacaagta aattattgat caattggact
    27841 ttccgatcat cggtgaaatt gatttcttgt ccatcttcga tttgctctaa ggccatttga
    27901 gtgattgtga cagctccttc aacaatggtt tgtcgagcag acaaaatggc ttttgcttgt
    27961 tgacgttgga gcatggcact agcgatttct gttgcatagg ctaaatggtt caagcgtgtt
    28021 tcgattactt cgacacctgc tactgctaaa cgctcttgca gctctttttg caactcgtca
    28081 gacactgccg tcgtgtttcc tcgaagcgtc aaatcattct cattgaatgt atcatacggg
    28141 tattggctag cgatatgccg aatggctgtc tcactttgga tttctacaaa atcttgataa
    28201 tacgctacat cgaataatgc ctttgctgta tcaacgactt taaagactac tactgctgag
    28261 atttcgatgg ggtttccatc caaatcattg acttttaaaa cagaactatt gaaattccga
    28321 actttcaacg aaacagtcat tttttgtgcc aaaggtatcg taaggaaaaa gccattttct
    28381 cgaatcgtac ctaaatattg tccaaaaaac aaaattactt ttgcttgatt aggactgact
    28441 acagttgccg aacttaacaa caataacgtg atcaaccaaa gaaaaatact taaaaaaagt
    28501 gcccaaactt tcgcttgcca catacctaaa tagaagaaaa acaatccaac taaagctaat
    28561 acgaccaatc cgacaatccc tgcataacca ttgatataaa atgctctttt ttctctcatg
    28621 ctacttcctg cgctccttta tccttcaata aatatctaat gttacttttt taattttttt
    28681 ttcgaatacg cgccattaca ccttctagtt ctaactcttt gtcttcagtg atgtaccaac
    28741 cgcttgcctg tgtccctccg gctgcctgcg gtaaataatc caattttgtt cgaacccagt
    28801 gcttttcttc tgtaaaatag tcttctacca agacctctag atccatccct gctgaaagag
    28861 gcaaatcttc ccgaacagat ttaaagataa atcgttcctt cttcccatca tacttcaata
    28921 ttcctgtatc gataatcggt aaagaataat aatccagctc tttcttcacc gtcaataaat
    28981 gtttgaaaac ttcatggtac acttgctcta attggcgacc ttctgaattg ttcgggtaat
    29041 taaccatcac tttttctttg tctggatcat agaaggtttg gcttagttga gtgatataag
    29101 tatgtaatct ttccatttct gtttttaact tttctctttg accttcttct accttcatat
    29161 actctttcac cctgttttct ttttagatat tttctttctt attttactat agttatgtgc
    29221 gttaagcgat gaaaaacagc aaagattgga aggatctttg ctgttttttc ttaaaatatc
    29281 cagttgagta caggatcaat ctgtttcatt gtcctcttga tccgtcgtaa tattgatcga
    29341 tgtgtcttta gcagtgatct ttagcttttt ctgtccttgt tctaacaaag tatttgttat
    29401 tggctggtag tctttttcaa tataagattc accagacgtt tggatagtat agccactgtt
    29461 ttccatctca tgcaaagata aattgccacc gtttaggatg acttttcctt ctttgctgat
    29521 caaactgtct tctagatgga agttgctgtt ttcacctgtg acgatcaagt tatccacatt
    29581 ggcctcatct aatcgaagtg agcctaattc atctttcgca gttattgttc ctcctgtaaa
    29641 ttcattgaac gatacccagc catcatgtgt cttagtattc acttttttga ttgatacttc
    29701 gttgatatca attgcactat tattcccttc gattgtcaat tcagataatt catgatctgc
    29761 agggacaaca atagtgattt gtgcacttgt gtcttctaaa ctaatgattg cacctgttga
    29821 ttttttgact gcattcacag tcaacacgcc atttttctcc tttatttctg gcttttctgt
    29881 cttatcataa gtcgttttta tctcaaaatc caatccgcgt atgaccgaaa ctgtttgatt
    29941 ttctgcatgg atgacgattt tgtttgcatc cttgattttt tttgtctcag taaccatatc
    30001 gatcaaccga ggaccattat cccaagtcag actcttttga gcgcctaatg tataagttgc
    30061 acctgctagg aggccaccag taagcatcag accaataccg gtaatcaaaa aatgtttcaa
    30121 tctcaattta tgtgtcctcc ttcaaacgcc cgctgaatgc agatctcttt ttgtaataaa
    30181 acgtcgtccc atccatttaa caaagttcat catgacataa aaaagccctt ttgttccttt
    30241 cagcaacagt ggcgaaataa atactgcgct tcctgtggcg aacagaccga caccgataaa
    30301 gaataatcca ctcagaatcg attgtccaaa cacactgatt cctgagatga tcatgaatag
    30361 gccaacacct aagatactga tacataaaac atagatacta aaaatgagcg ctagtaaagc
    30421 aaccaatagt cccccaataa ccaatataaa ccccagtgct agtggaatga ggacaggaga
    30481 agcaaataaa ccgataatga ttaaccaaat caggcgaatt tgtctttttg gatttttcac
    30541 taactcgttt tctgcttgtt cttcttctga aaagtaactg atttttaatt ttaaggcaaa
    30601 ctgctttggt gtaccatatt catcaatgat ctgatctaat gtttttccag aatctaaaaa
    30661 atattcctca taatactgga tcaaatcaaa acgatcgtct tcaggaattt cttgaagatg
    30721 atccacaact tcattcaaat attcattcat ttgtgctctc ctccaataac cggtctaaag
    30781 aatctcgaaa aacaatccat tcatcaataa tttcttctaa atgacgactt cccattggtg
    30841 tcaacgagta atatcgacgg tttctgcctt caaaaggttc atcataggtc tcaagatatc
    30901 cttctttttt caatcttctc aaaaccggat atagtgtaga ttcactgaca gagagacttt
    30961 cttgtacgat ttttgttaag gcatacccat acatatcttc cttgcttaat gctgctagca
    31021 cagtaccatc aagaagaaca gttggaatct ggatgtgcat tacgtcacct tctattctca
    31081 agtgtaatat tttatttcaa tctatattat acaacatata atatagtttc aaaatcagtt
    31141 tgtcaaatac aaaaaaactg actaaaagat ttgatccttt ttagtcagca aaaaatggct
    31201 tctctttgat ttttattata acagtggcat aatccaattt gtccacatcc agctcagtgg
    31261 catgataacg atcattgcag gtatcatgtc agctactggg aacattttca cgttgatcat
    31321 tctaaaaccg gtagcgacca tcaagaatcc gccgcaagct ttaaaatcta agatcatgtc
    31381 tggtgtagtc aatggataaa taaattttgc caataagaac aaaatataaa aaataatgaa
    31441 ttggggaatt gctatcacag aaacgacata ccctaaatta cacgcaaaaa tagctgctgt
    31501 gaaaaaatca aggacagatt tagaaatcaa aatcgtactg tctcctgtca tcccagaatc
    31561 taaacttcca tagatccccg taccacttgc acaaaataga acaatgaccg tcactaatgt
    31621 gctgataaat tcatcgtggg tcataccaag cttttcacta gggaagattt tagaaatagg
    31681 tacttgcatc agcgcagccc ccttatttat cccattacct aaatgaacaa tcaaaccaat
    31741 cgctgttcct atcactaaag caaaaataac agccggcata tttttcatcg gcgcaatgga
    31801 atagattccc atccccatcg aacacacacc aaaaaccatc gtcagctgtg ctttaaaatg
    31861 ttcacttaat ttatttccta ctaatccgcc aattagtccg ccaaacaaaa cggaacctac
    31921 attgatcaaa actccagttg gcatcaaaaa actcctctct ttttctacga agcgaaaaaa
    31981 atcagaaaaa tttctgtaat tattgctttc gctgcaagaa agattgtaac agacagagaa
    32041 gattacacca attcattttt tgtgttaaag tattctttat tggaataagt aggaggaata
    32101 tgatggatat ccataaattc gaagtttttc ttgatttagc cgaaacaatg aatttcacca
    32161 aaactgctga tcgacaattc accacccaag gaaatatttc taaacagatt ctttcgttag
    32221 aaacagaatt agacgtcaaa ttattcgaac gcgcccatag aaaaatcgaa ttgaccgaag
    32281 ctggaagctt attgctccca tatgtaaaaa atgtcgtaga acaataccat gctatgcaag
    32341 aagtgcttga aacctatacg aaagacaaca atctttcatt aaatatctta acgataccga
    32401 caatgatcaa ttataaaggt ttttccaaaa tcaccgagtt tttgaaacgc catcctgaat
    32461 ttacagtcca gttgaaagaa gtggaaagtg tcgaattaac ttacaatgac gccaatgaac
    32521 atagtgacac gatccacttt gctagaagtt tccaggcccc accaagttcg attgagtggt
    32581 tgcctactga aacagacgat tttgtagcag ttctacctaa aaatcatcct ttagctagta
    32641 aaaagaaatt ggatctaagc gaactgaaaa aggagaactt tttgctcctt ggccccacta
    32701 cgaacttgta tcaacctgta ctagatctgt gtcgcgaagc tggatttgaa ccaaaaatct
    32761 cttataaagg agcaagaatc gacttgatca tcaatatgat tgcaaatgac atggggattg
    32821 ctattgttat ggaaaaaaca gtcaagaatc tattaaaaga aaatactgtg attctcccta
    32881 tttctccaac aacagagagt tatcttgcat ttactcgtaa agtcggtgag cattctttgg
    32941 cttcagatac attttgggag tatttaaatc aagaagaaaa ctaagcccta cattttctgt
    33001 ccatctatcc acccaaaaaa agagagcgac gcatgacttt cgctctcttt cttgcatcat
    33061 gtccttgttt caatgacacc tctaacattt ctcgtagttc aataagaaag cagcgatttt
    33121 tatgccaaaa gaaccgtaag aacatttccc taatcacaaa aaaacgacta aaaaccccta
    33181 aaaaccttca atagaatttt taggggtttt attctttttc ctattgttta cttaaccgta
    33241 tcattgctgt cgttcctact ccttcgatac tgctataatc aatacgtcca tcatgcagtt
    33301 ctagaatttt tttaaccagt gaaagtccta gcccattccc agaaatttga cgacttgtat
    33361 ctccttgata aaatttttca aacattcgat ctctggtctc ttcattcatt cctacacctg
    33421 aatcggtcac ttccagtaca atctctgtgg ctgtctcaaa tagtttgata tgaatctgtc
    33481 cattgatttg attgtacttt atggcgttat ccatgatatt cagccacact tggtacaaaa
    33541 actcttcatt gcctgtgtaa tttactgcag caagttcgat atccaattct agctgttctt
    33601 tctcccattt aggctgaaaa aaaacgatta cttctctgat ttgttcatct aatcgatact
    33661 ctttcttttc cagttggata cgctggtttt ccagtttgtt caattttaag atgttctctg
    33721 ttaattgtga aagctgcgtg atactttcga taatccgata taaaaatatt tggcgttctt
    33781 cgtcagagag atttggggct tgtaacaatt gaacatatcc ttggattgtt gctagtggtg
    33841 ttttgaattc atgggataca ctggacacaa agtcattcct cagtgtctcg atactattga
    33901 gttcttggac cataacgttg aaatctttat acagttgctg tacttctgct actttctgct
    33961 gttcatccat tcgaatcgaa aaatctcccg ttgctaccag actcatattt gtccctagcg
    34021 cactgattgg atgcaggata cgccgcccaa caaaaatcga tattcctgta cccacgacca
    34081 tactgaaccc cgcaaagacg aataacgggt ataacgttgg ttttccagcc gattcagcag
    34141 gcgagataga atgtcgagac aataagaaaa taaaaccgag aaaacacagc agtgtcacta
    34201 agatcgaaat gaagacaata ctagcaaaat agatccataa ttgtgaaaat agcttttttt
    34261 tcatctttaa ataaccgcct tatagcctag tccccgtact gtgatgattt gaaaatcttc
    34321 cgtcttttca aaacgggtcc ttaaacgttt gatatgtaca tcgatcgttc gttcttctgt
    34381 attcgtatct aatccccaaa tatcatccat caactgttgg cgagtgaaga tcttgttggg
    34441 ataagacagt aatttataca gaagcagaaa ttctttttga ggcagaatca acgaatgctg
    34501 atcttgtaac actgtataag catctagctc taattttgtt tgtccgatta tcaagctact
    34561 gctgtaattg atctttgaac gtcgaagcaa tgcttccaca cgtaggatca tctcattgac
    34621 atcgatgggt tttgtcatat aatcatctgc tcctaatttg aacccgcgtt tcttatcctc
    34681 aaaatcggcc tttgccgtaa tcattagaac aggtgtgtca aatctggtgc tacgtaatga
    34741 gtctagcaag tcatagccat ccattttcgg catcatgata tctgtaatga ttaattctat
    34801 acgtgtcgct tctagttttt tcagcgcatc ctctccattc aatgcggtga tgactgaaaa
    34861 gcccgctcgt tccaacacag aacggtaaag caactgtaat tgcggatcat cttctactac
    34921 taaaatacga ttcattgatc tccctctgtt ttttgaaaaa tttctagatt ttttcttgta
    34981 gttcatcttc agttaacatt tatttcgtat actacgatca ttgttctgat atacttatta
    35041 gtataccgaa tatttcaata cgggagaagg gtgattatgc ttcaattaaa agatataaaa
    35101 aaatattata aagtcggcga aaccaccacc aaagcattgg atggtgtgtc tgtcgctttc
    35161 agacaaaaag aattcgtagc gattttaggt cctagtggtt ctggtaaaac gactatgctg
    35221 aatgtgattg gtggactaga taattatgat tccggtgata tggtaatcaa cggaaagtct
    35281 actaaagact ttaaggacag tgactgggat gcctaccgaa acaactctat cggtttcatt
    35341 tttcaaagtt acaacttgat tggtcatcta ggcattattg aaaacgttga acttgggatg
    35401 acattaagtg gtgtttctaa agacgaaaaa cgaaaacggg cagaagatgc attgcaccga
    35461 gtcggcttga caaatcatat gcataaaaag ccaaatcagt tatctggtgg acaaatgcag
    35521 cgagtggcca tcgctcgagc actagccaat gacccagata tccttttgtg cgatgaacca
    35581 accggagcgt tagatacaga aacaagtatc cagatcatgg aattgatcca agagttatcg
    35641 aaagaaaaat tagtgatcat ggtcacacat aatcctgaac tggcccatca atacgcagat
    35701 cgaatcattg aattttctga tggtaatatc ttaacagatt cccatcctca catcgaacga
    35761 ccaaaagatg atcaatttaa cttacgcaga acaaagatga gtttttggac agcgttaaag
    35821 ctttccttca acaatatccg aacaaaaaaa ggacgtactt tcttgacctc ctttgcttcc
    35881 agtattggga tcatcggaat cgcgatcgtt ctgtcccttt cttctggatt ccaaaaacaa
    35941 atcgataaca cgcaagctga aacgatggcg aaatttccga ttactatttc taaggtaacg
    36001 actaaccaga ctcgtgacga tgccggattg ggtgcaagca aagcggatta tccagacagc
    36061 aaaacgatta ctgcaaaagt cagcgaagaa gataaagcac agcacacgaa taagattgat
    36121 caaacatacg tcgattacgt aacagacatc gatcctaact taagtaataa tatcggattt
    36181 acccgtacta caggaatcaa tcttttacgt gatgtaaatg gcaaagtaca gcctgtcagc
    36241 ttctcgaatc aaaatcctga tacggaatct ctttctttat ctagtacgat gtcagcaatg
    36301 acaggtgtgg gagtgtcaag tttccctaca cagcttgaca ctagcaaaga aaacttttta
    36361 aaagacaatt attctttact tgccggaagt tatccagcct cagcaacaga tgtcgtgctg
    36421 atcgtagacg gcaataataa tactaatatc aacgcattga agaatcttgg atttgatgtg
    36481 aaagaagacg aaaaacttga ttttgatgat atcgttggta caacattcaa attagtaaac
    36541 aacaatactt actatacaaa acttccaact gggaatttta tccctaatac agattatgat
    36601 gcgatgtatc agaatgcttc tgatgaattg aagatctcgg ggatcctacg cgtgaaatct
    36661 tcttctacaa tgaatctctt atctccagga atcgcttata gcgatcaatt aacgactcaa
    36721 atcgtcaacg aaaacaaaga atcagaaatc gtcaaagctc aaagagacag tggtgtaaat
    36781 gtcttaacta ctgaaaaagt tgacgaaaat gccaaacaaa cattgcttag ttatttaggt
    36841 ggagacagct tgccttcaag tatcatgatt tatccgaaca attttgaaga caaagaaaag
    36901 atcttggatt atctagatga ttacaataaa ggcaaatcag atgaagataa aatcatttat
    36961 actgatttag ctggaacgat gactgaactg actggcggat tgatggatgc tatcacttat
    37021 gtccttattg cttttgcagg gatctcttta gtaacaagta tgatcatgat cagtatcatt
    37081 acttatacat ctgtgattga acggacaaaa gaaatcggtg tactaaaagc tttaggtgca
    37141 cgtaaaaaag acatcactcg cgtatttgac gcagaaacgt gtattttagg tatttcttct
    37201 gggatattag gtgtgtttat tgcatggcta gctacgttcc ctatcaatag catcttatac
    37261 aacatgacgg atctaaaaaa tgttgctcaa ctaaatcctg tccatgcgat tattctagtg
    37321 atcgtctcta ctatattgac gatgcttggc ggtcatcttc ctgcacgaat ggcagcgaaa
    37381 aaagacgccg ctatcgcttt gcgtgcagaa taaggttgtg acaaccacat ttagatttga
    37441 gtcataagag cgctgaaata gtagaaaaca gcaagaaacg aggaagtaat atgttccttg
    37501 tttcgcgctg ttttttttaa ttatttttta taattctggt atacttttct ttcctttgta
    37561 ccacaactct tctggcgtat acctgtcttt tccatttatg aatactgttc catctgcttc
    37621 aatcgttact tcagtcccaa tccacatatt acgagaaagt gcgagaagat tttttctttt
    37681 tcctcttcct ttttttccaa attgcaaggc atcggttatc tcttctgtcc aatgagattt
    37741 cacgtatttt acttctccgt tttcaaatgt aactttcaaa atattcgcta tattttccac
    37801 ttttactgct ttaggaaatg actcctccat ttttttcact ttccttcctc caacactaag
    37861 tatgcgcttt cttttagtga taagtatatc atagtttatt ctattatcaa aaaaacgcat
    37921 ccattccatt gtagaatact ttaccgaaca agttgaattg ttttcctctt tttcaccttt
    37981 tactatagat aggtaaagac aactaaggag gaatctctca tgacagatca accgtatgat
    38041 ttaagaaaag tcatcgaaga actaaaaaca cttccaggtc aatatcacga aacagatatt
    38101 gaaatcgatc ctgaagcaga attatctggt gtttaccgtt atatcggtgc tggcggtaca
    38161 gtaaaacgtc cgactcaaga aggaccagct atgactttca acaatatcaa aggtttcccc
    38221 ggcacacgtg tgaacatcgg aacaatggct agtcgtaaac gtgtaggtga aattttgcac
    38281 catgattatc gaacacttgg tcgtttcttg aaagacgctg tagaaaatcc aatcaaacct
    38341 gtgaaagtag acaaagcaga cgctccagca caagaagtcg ttcatctagc taccgatgca
    38401 gacttcgata ttcgcaacat tttgcctgca cctacaaata ctcctgaaga tgctggtcca
    38461 tacatcacaa tgggtgtagt cttaggatca gatccagaaa aaacaatgag cgatgtaacg
    38521 atccatcgta tggttctaga agataaagat acgatcggta tgtacatcat gcctggcgga
    38581 cgccacattg gtcacttcca aaaacaattc gaagccttga ataaaccaat gcctatcact
    38641 atcaatattg gtttagatcc agcaattgcc attggcacga ctttcgaacc acctaccact
    38701 ccacttggct acaatgaact atggtgtgca ggagctttgc gtaacgaacc tattcaaatc
    38761 gttgatggta tagcagtcga tgaagtgggt attgctcgtg ctgaattcat cattgaagca
    38821 gaaatcatgc caaatgaaac aatgcaagaa gatatcaaca cgaactcagg aaaagcaatg
    38881 ccagaatttc ctggctatac tggggatgct aatccagctg tacaagtagt gaaagtcaaa
    38941 gcagttactc accgaaaaga caatccaatc atgcaaacaa cgattggtcc cagtgaagaa
    39001 cacgtatcta tggccggtat tcctacagaa gcaagtattc ttgccttggt cgataaagct
    39061 attccaggaa aagtagtaaa tgtctacaat cctcctgcag gtggtggtaa attgatgacc
    39121 atcatgcaaa tccgtaaaga aaacgaagcg gatgaaggta ttcagcgtca agcagctatt
    39181 cttgctttat cagccttcaa agaattgaag acagtcattt tagtcgatga agatgttgat
    39241 attttcgaca tgaacgatgt agtttggact ttcaacactc gtttccaagg agataaagat
    39301 atcatcgtga tcccaggaat gaggaaccat ccattggacc catcagaatt accagaatat
    39361 aatcctggct ctattcgttt ccgtgggata agtgcaaaaa ctatctttga cggtacagtt
    39421 cctttcgata tgaaagaaca ttttgtacgt gcaaaattcc aagaagtaga agattgggaa
    39481 aaatatttga aataatgaat gatctgcaac tgttttgaca tcataaaaag gagtgcggaa
    39541 aatggaacaa ttggcacaac ggaagaaaaa aatcgtgatt ggtgtaagtg gcgcttcagg
    39601 caccatttat gccatcaacc tagtgaagaa actaaaagaa tatccgatga tagaaacaca
    39661 cggaatcatc agtccatggg caaaacagaa cttaaagctg gaatccgact tatcactaac
    39721 tgaattcgaa agcctgtttg attaccatta ttcaaacaaa gatttaggtg ctgctgtcgc
    39781 cagcggatca tttttgaccg atggaatgat catcgtaccg gcaagtatga aaacagtcgc
    39841 aggtatctct gttggtttag gcgacaacct gattgcacgt gctgctgatg taacaatgaa
    39901 ggagcaacga aagttagtca ttgttcctcg tgaaacgcca ttaaatgcca ttcacttgga
    39961 aaacttgact aaattagcac gactaggtgt gcaaatcata ccaccaattc ctgcttttta
    40021 taatcagcct caaacgatcc aagatcttgt taatcaccat acaatgaaac ttttggatca
    40081 atttgaaatt gtaaatgagc attcaaaacg ttggcagggt gattaatatg gaaaaagaaa
    40141 cgatagttga agtaaccaaa ctaggtttca cgatccaagc tgctgttaaa gtaatcggta
    40201 aagacttatt gatcacttta acaggcggag atacaccaca tatcggaaca gtcactacat
    40261 tttctcgtga tacagagatg caaactgtac gtttccctag ccacgatggt cggttccata
    40321 aagacgatgt cttagccgaa aaaatagctg tgatcatcaa aactgccgtt ccgggaagtt
    40381 gtacgatcac ttccggtatc catgttgacc atatctccaa tgcacaaatc aacgcctctt
    40441 ttccgatgtc agaagagtta ggttacaaga ttttagattg gttggaaaaa atagatttcc
    40501 atatcaaaga accagtttat tataaagatg gtgaaaaacc attatgaaag aagaggttgt
    40561 gaaatcagcg ttatgacctg agtatgagag aaaaaaatgg aaaaatagct tctcatattt
    40621 tgccgttttt gagctaatac cgagggacag ctgattgaac gccgtttatc aagaggttgt
    40681 gataaagccg cttcgtttcg agtagtaaga aaaattttgt aaacatgggt ttccacattt
    40741 ttgcataaat ttgacttact accgagaaac tggcttttga acaccgttta tcaagaggtt
    40801 gtgacacgtc ttttgtcaca acctctttta tttttgcttc actgtaactg ctacaatatc
    40861 accgatttct tttccgatgt ttttacgaat atcttttcgt agtccaagga tgtagcagac
    40921 tgaaccatct ggatttttca cgcccatatt cacgatactg ccttcatacg gcacattgtc
    40981 aaaagttgct tggactttaa ctcttccagc accgaattct tttcttatat catacggaaa
    41041 aataatatat gccccgccct tgccgacttc ggaagcatag atttttgatt caaaagaata
    41101 aatttccctc attttatttc tcctattttt tacatctagt atagcatagc aaaaaaaaga
    41161 aggaactgtg acaaaagtct tgtcgcagtt ccttttttcg aataaacggt gttctcctgc
    41221 caactcctcg gtcacaagtc acaatagtca acaaacatga gaagctgttt tttgaaattg
    41281 ctccttgtga ctcagagtta aacggcaggc tcacaacctc ttccttaaac ggtgttcaaa
    41341 agcttgcatc tacggcacta agcccaatca gatgaaaaat atggagtgct atttttatct
    41401 gattgttctt actgcttgat gcagagcagc tttttcacaa cctcctacat ggtgttcaaa
    41461 atattattcg ccttttgctt ctttcactaa ttttgcgaat gcttgttgtt ggaattcagc
    41521 agctaaggct ttcgtacgtt gttcttgctc tgctttgctc atttttggat tttcagtcat
    41581 tgcttttacc ataccgtctt gaacataagc ttttgcttca ctttccatcg tactttgctg
    41641 tttcttcact aattcagcta acttggctgc ttggtccgta cttaattcta cccatgtctc
    41701 tgggattttg aatgtattca ctcggctgtc atattcgtga ttgttatcag caatattgaa
    41761 ccagaattta tacatgtcgt ttttgtattc ccaataagta gttttcgtca ctttttcagc
    41821 atcgccgtct gtcttttcta ctttatttgt ttcgttatcc gtaccagttg cttttggttt
    41881 ttcttgtttt tcatttgttc ggtaaagata cactttttct tttccattcc ctaaagcttt
    41941 atacaaaagc atatctgctc cttgggaatc agcactggaa acaatttttt ctgtttttgt
    42001 ttccgttact ttttccattc caaagtgatt ggacaaatta gcgacgatca aacttagtga
    42061 agcgacgaag attagtccga aaatcaaaga taaaaaggtt tgccatgttt ttttagcgaa
    42121 cacgttcgtt aaagcaaaag caagcaagct gacaatcaaa actccaataa tcatgcatta
    42181 tcccccgctt tcattagaac aggttctttt ttcttcaata aaaaggcaat aataaagcca
    42241 agaatcccaa agatgatcgc tacgataaac gcggcatggt aacctgacag tgtggcattg
    42301 attgcctgat ctttataagc taatggcgta ttatccaata cagatttgct tgggatattt
    42361 gcttttgtca cattcgtcaa cacactgatc aaaatagctg taccaatcga gcttgcgatt
    42421 tgacggaatg tattatttac cgctgtccca tgactgatca agttatttgg caatgcattc
    42481 ataccagaag tcgttaccgg catcatgacc atggagatcc caaacatacg gatcgcataa
    42541 aggatcacga tatataaaac tggagtcgtt tttgtcaaga aagcaaatgg tgcagttgcc
    42601 gctgtcaaaa tgaacatccc tgtgatggct aaacgtttcg cgccgtattt atcgaaaatc
    42661 gcacccgtca caggcatcat agcccccatg atcaatgcac ccggcaacaa catcaagcca
    42721 gaatggaatg ctgattctcc acgaatattt tgaatataca atggtacgac catttctgcc
    42781 cctaccatcg ccatatttgt tacaccagct aaaacagctg caatcgtgaa tgtagatgat
    42841 ttgaatacac gaagttccaa gaatggatgt tccaaatgta attgacgcca tgagaacaag
    42901 gcgatgaaaa tcacaccaac aactaagaaa ccaattactt tcaagcttcc ccagccgtca
    42961 tctcctacac tagaaaatcc gtaaagtaaa ctaccgaatc caatcgttga taaaatagcg
    43021 gataaaatat caatgctagg atttgacaat ggaataacac ttttcattag gaagaatgct
    43081 aaaaccaaga caagaacaac gattggcagt accataccaa acaagtcacg ccaatggtag
    43141 ttatcaatga tataaccaga taatgtaggg cctaaagcag gagcaagtcc aataacgatc
    43201 cctgacatcc ccattgctga tcctcttttt tctggcggga aaatagatag catgatattt
    43261 tgcaataatg gcatcgatac tcccacaccg caagcttgga tcaaacgacc gattagtaat
    43321 gtactaaaat taggtgctgt aaagcaagta atcgttccga tcaaaaagat cgtcatggca
    43381 gaaagatata aatttcttga actgaattta ttgatcagcc atgctgttat tgggatcatg
    43441 atcccgttga ctagcaagaa accagtcgtt aaccattgca catcagacgc tgaaatatcg
    43501 aaagctttca tcaatgtagg aaaagctgtc gtcaataagg tctgattcaa aacggtacaa
    43561 aatgtaccaa ttaataagac aacaactaaa agtgtccgat tatatggttt tccgtaaata
    43621 tctacgggtt ggttgttact catatttctc cttctttctg aattttttct gaaactacta
    43681 gaatacttta gtcctaaatt cgctatttgt caacctactt gacacataag ttaatacgga
    43741 tataattaag ggaagcaaga ggacaaaagg agtgacaaaa atggttgggg cttcccttaa
    43801 acgtttgttg gaaaatgatc aattggttgt tggcatcagt gaattaagtg atatcgtagg
    43861 tgtgtctcca cgtcaattaa ggtattggga acaaaaggga ttcattcgtt cgatcgaagc
    43921 tgatgcgaat tgtcctagaa aatatcgttt acctacagtc ataaaagtag aactgatcaa
    43981 aaaatattta gatgacggat ttactttgtc aaaagctgca gaaaaggcag aaatccgcca
    44041 aaagaaaatg caccatgtcc gtaaagtttt ttcaaaagca atcaaagata tcgaattgat
    44101 cgacgaacgc tatacgattt tacaaatcgg taacttcaaa gaagagaatg ctaagatgta
    44161 catcattcat gacaacgaga cagacgaatt aacttacaag ctccttcctg tcgatgaaaa
    44221 agctgactac gaacagttgt taaaagaact ctaaacgcca ctaatctcct aagtaccaag
    44281 ctttttaata agtagatgct acataaatac ttgaaaaaag gcctgtttga acataagaat
    44341 atccgaacgg tgatctgcgt ttctttcata tagaaaggaa ttctccatca ctgttcggat
    44401 atttttttat ttttttcggt tactttcttc taatacatct gttagccaat ttgcatattc
    44461 tgtttcccga ttgatcgcat gcgctaaaat caggtagtgt ccgtaatttt catgtttttc
    44521 tttttcagta ggaaagatcg ttttttttcg ctctaataga tgaagtcgtt tttcttcatg
    44581 aaggtcacgc tgctgctgaa taatttctac taatgccgga tcattcacgt ctttgacgaa
    44641 atacaatttc agaacaaatt cgtctcgatt aaccggtagt tcattggtgg gtgtatgaat
    44701 ccactctgcc aattgttcct tcccttcttc tgttaattca taaactttct tttccaattt
    44761 ttcacctgtg atctctactt gatgctgaat gattccttgc tcttccattt ttgcaagttc
    44821 aggataaatc tgactatgtt ttgcttgcca aaattctcct atttcatttt tgaacgattg
    44881 attcaattca tatcctgtca tcttcttctt acttaataac cctaacaaaa tatatcgaag
    44941 tgtatttttt cttgccatca gttttccctc cttgacaaat ctttaaatcc attctatcat
    45001 aaatgagtaa tcgaaaacat gtagtttatt acatgtttta atttataagg agatgcttaa
    45061 acatgaaaga atttaaagaa ttggaagatt ttattggaac tcactttatc tatacgtatg
    45121 acaatggttg ggaatatgaa tggtatgcca aaaatgacca tactgttgat taccgtatcc
    45181 atggcggaat ggtcaaaggc cgttgggtaa aagaccaaga agctgatatc gttaaattga
    45241 cggacggcgt attcaaaatc acttggacag agccaacagg gacagacgtg gcattggact
    45301 ttatgccaaa tgaaaagaaa cttcacggaa tgatcttttt ccctaaatgg gttgaagaac
    45361 atccagaaat cactgttact ttccaaaacg aacatatcgc agaaatggaa gcagctcgtg
    45421 aaaaatatga aacttatcca aaattacttg ttcctgaatt tgctacgatc acttatatgg
    45481 gtgatgcagg tatagacaat gaagatgtta ttagcgaaga accatacgat ggaatgccag
    45541 atgatatccg tagcggaaaa tacttcgatg aaaattataa acgagtaaaa taaggttgtg
    45601 aaagagaggt tgtgaaatca gcgttctgac ctgagtatta gagaaagaaa cggaaaaata
    45661 gttcctcatg ttttgccgtt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt
    45721 tatcaagagg ttgtgaaatc agcgttatgc tttgagtaat aagaacgcta gttagaaaat
    45781 agcttttcat attttctagg ctagtgggct tattaccgag aagcagccgt ttgaacaccg
    45841 tttatcaaga ggttgtgaaa tcagcgtttt gacctgagta ttagagaaac aaatggaaaa
    45901 atagttcctc atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc
    45961 gtttatcaaa gattgtaaca actgcatatt cacacagtta aattagtgac ttcaaaatga
    46021 aataatgctc ccagcaaaaa attaggctgg gagcattatt tcgttattct gttcatcaaa
    46081 aatcagcgtt ttgattccat ttcatcacaa gttcctcctc atttactgct tcatcttttt
    46141 gctgtgtttg aattgtaaaa ccttgtgaaa gatagaaatg aatagctgat tgattttttt
    46201 gatagactgt caaagtcagt gttttattgg tttcttttat ttttttcatt agttgcgtac
    46261 cgattctttg ttgccggtat tcccttttca caaagattcc tgcgataaac tgatcaatca
    46321 ccccagcaaa accaataatt tcttgatcta tttctgctaa gtaaatcact gcatctggta
    46381 aagctttcct aacagattct acttgattct cccaataagt tgatggaatg aattcatgag
    46441 aatccaagtt agcatgcagc caaatcgata gaacagattc cagttctttg gcagacaatt
    46501 ttgttgttgt gcggatcatt ttttctctcc ttacttattc attgatttta aaataaaaac
    46561 catttttttg aagctctttt acaagttttc gcccctcaaa aaagtatatc ttctcttgtt
    46621 ttacttttct aataccaaaa gaaacagcaa ctttaggatc atcagctgaa acgattcgat
    46681 aagttccacc aggcaattga tccagcttga ctttcccatg atggtcgctt tgtactgaaa
    46741 acggcacacc atttctagta agtgtttttt tcttatgttt caaaatgaat gaactaccat
    46801 ttaccggttt ccctgacgaa tcactaataa taaatgaaaa atcaagcact tttttactta
    46861 atctatactg atagattagt cgtccgataa acactaacag tatgactaca gctattatcg
    46921 ttcctgcaag aataaaccat tgccgcatgg ttctttcctg atttcctttt ccttcttctt
    46981 tttttaccga ttcgttataa ggaatccgat gtcctgtcac tagcaaacga tgcgtgttga
    47041 tcatatacgg cgtacatgta agcaaggtaa ctaagtcttc tccttctgaa atcgttagaa
    47101 cagacgtgtc atctggcagt accacttgta tatttttaac ttgataagcc aatttctccc
    47161 ccaaaacagt caaaataaac gtgtctccgt tttctaaacg atctaattcg gtaaataacc
    47221 gtcgattagg taaaccgctg tgagcagaga tcactgaatg cgtcgaattt ccaccaagcg
    47281 ggaaagaggt tccttgcaag acgcctgctc cattttcaag gatcctacca ttgagccgat
    47341 cataaagtga aatatttaat tggatcttag gtatattgat acttccgatc agccattcct
    47401 ctaattggct ggatgttgta ctttcatttc tgttcttttc attgaaagga tcagtaccag
    47461 gattgaatcc ttggtcagtt aatcgttggt ttttctcctc cattttcttt cgctgcactt
    47521 caatttcagc tgcggtccgt ttttgtactt gttcgatacg tttttgatca ataaaactat
    47581 ttaaagaatc cacataaaat ggatataaag cgatcagtaa ccctacgacg atacatatta
    47641 ataaaaacaa acgaaacaaa agtcgacgag actgtttttg cttactctgc ttcacatgtg
    47701 tcctcccttg caagtttttt tgttctttct tttattatat gggttcagga aaaaaataga
    47761 aaatgaaagc cgatgaggtt gtgaaaaagc tgtcctgcat caagtaataa gtacagccca
    47821 aaaaaatagc tactcatatt ttttgttggg ttgggcttat taccgcagat gcaagctttt
    47881 gaacaccgtt taaggaagag gttgtgaaaa ctgcgttatg ctttgagtaa taagagcgct
    47941 agtcagaaaa tagcttctca tattttctag gctagtgagc ttattaccga gaagcagctg
    48001 attgaacacc gtttaccgag aggtcatgaa aaagctactt tgtttcgagt attaagaaaa
    48061 attctgtttc aaaacaagga gatgcaacaa aacttatgtc gcatcctctc tccttagaac
    48121 tttcacttat gtttttcttg taaaattcat aaatattttc atgaattttc taatttcttg
    48181 cctacctctc gcataggata tatgatgaat ttatctaaag tttgcaaagg agttcgacaa
    48241 ttgaaaaaat atggcccatt tttagcaatt tttttcattg ctttggcatt tctcatgcct
    48301 catttattca cacataaact aattttgggt gctgatgccc cattccatta caatcggttc
    48361 tatgatgccg cgcaacaaat taaatatggg gatttccatt attttccttc cctttatgga
    48421 ttccagcaaa gtggcaggat cgtgaatgcc gtttacggcc catttttcgc ttatttacaa
    48481 ggattgatcg tgttgcttgc aggtagctgg tttcattatc agattctttc aaatttcatt
    48541 ctttatctta tttcaggatt ttctatgctg attttactga gatatgtcaa tgttcgaaat
    48601 tgggtaagtg taccagtagc cgttttattc atgagtacgt attctattca atactggtct
    48661 atcaaccaag gattcactag ctgggggact tgtttttatc ctttatgtat gatccctatt
    48721 gctgatttcg tccttcataa aaagttccct gttatcaaag tcgctgtctc agtgggactt
    48781 atgacacaga tccatttact gtcttcgatc atgttagctt tgatgtatat ccctttttat
    48841 ctttattact tttttaacca gactgaaaaa ggaaaagcac tgcttcaact gattaagtcg
    48901 gtcttgcttt attttgttct gacagcaaat atttgggcag gattagctat tgtttacagt
    48961 gggaataaga ttcttcctcc ctttgttaac agagaaatga gtcaaaaggc gatagatctg
    49021 gatggcagtt attggctcca ttaccctaag gcctttccta tattgatatt atcaggtttt
    49081 ttgcttttta ttttctttca acaaaagaac accttattcc aaaaaatcgt gttgtacaca
    49141 agtcttgttt tcttttttct ttctactagc ttgattccat ggacgacatt gatcgaaaaa
    49201 aatgtgcctt ttattagttt gatccagttt ccatttcgat ttttcgtgcc atttacgcta
    49261 ttgcttctac tttaccttgc cctttcactg aaccagtggt ctgaacaaaa gtggttccgt
    49321 cttttaagta tatttggcat tcttattttt tcagcacaga cgatccagaa cttataccag
    49381 catctggaaa agtgggaaaa tgaaacattt gtcagtcgac atacttatct attcgatacg
    49441 ccggaagaag caaggaaaac attcttcagc aaagatatgt ctaccagctt gttcgttttc
    49501 caaaaaacca cacctgacta tctgccaatc tataaggaga caaaagacaa taaatacgac
    49561 cggtataacg aagaaatact agaaaacgaa acaactttta taaaaacaca tccaggcggt
    49621 cttttgacaa tcaagtggaa aaacactggg gacaaaactg ttcatatccc tgttatcgtc
    49681 tacgatcgga ctgtgctaca gcaaaatgga aaaacattga ctgattatga agtaacagat
    49741 atcgggaccc caatcgtgaa gcagcaaaaa ggaatcaacg agctcacgct tcattatcaa
    49801 acgccaatct atttctattt cattctttct cttactttga ttggctggtt taccttgcta
    49861 tgtttattta tttatcatag atacaagctt ttacgtgctt aggcaaaagg gataaataca
    49921 tccgatcaaa aaactctcca aacggtataa tcgtttggag agttttattt taatcagttt
    49981 ttttatgtta tatgttatat tttttattcg ttcacatcta attcgatttc caccccgtag
    50041 tgatcactga tgattggact atcgatccca tcaaaaatcc gttgatattt tttaacctgc
    50101 attccttcag ggacgaaaat aaaatctata cgcaattttt ctgtattttg ttcccaacca
    50161 tctattttct tttccacagt agctgctttt cctttttcat tcgcgacaac atacgaatct
    50221 tgaagaagaa ggcaactttc tcttacagta taataacctt gagagtctac tggattattg
    50281 aaatcaccta ggaaaaaaag tggtacgcga gtttccaaaa agtagttttc caagtttttc
    50341 cactcaaaat aaaagccact ttttttatct gtccaccaag aaaagtggca gctcgagaca
    50401 aaaatgtcct gatcatctat ctttgttttc ccaatcaaga tcttgcgtgt ctgataatct
    50461 tctttctttt gaaattggga gacggtaagg acctgacttt ccaatgtttt tttcgtaagc
    50521 agtgcatttc cttcttcata tttatcataa ccgatgtggc tcatttccca actccaaaaa
    50581 tactcttttc cgtgttcctt taaaagctga actaaacggt aggcaaaatt atctttacgg
    50641 ataggtactt gattctttac cggacagaac ttcattagct tcgcaggttc tacttcttca
    50701 gattccagta attggttgat ttcttgtaaa gcaatcactt cataatcttc taacaggatc
    50761 ttatctgcta attcttgcaa ttttttctct ggttctggct caagccagct atgcgtgttg
    50821 agtgtcatga atttcattgg ttttctcctt gtcttcattt cgaaacaaaa gggttgtgac
    50881 aaaatttttg tcacaacctc tccagcaaat agtgttctcc tgctaactcc tcagtaataa
    50941 gctcaatgga ccaggagcta ttttttgttt ggctgttttt atcacttggt gcagggcagc
    51001 tttttcacga cctctccact aaacagtatt caaactgctg cttctcggta ataagcccaa
    51061 ccaaacgaaa aataggagga gctatttttg tttggctgtt cttatgactt gatgcaggac
    51121 agctttttca caacctctcc ttacatttta atggagccga tcactttacc agctgtcact
    51181 gtacctaatt gcttgatcac tacttcttct actttatcgt ttgtaaatac gatcatgatc
    51241 gttggatctt tttcttcgtt ttggatcaca tcaagatcca tctcagctaa tttcgttcct
    51301 gttcctactt cttggccttc tttgacagca atctcaaaag ctggctgatt catttggact
    51361 gtatcgattc ccatatggat caaaacttcg ataccatctt tcgtttccag cccaattgca
    51421 tgtttgctag ggaaaatact tgtcactttt ccagcgatcg gcgcaacaac ttctcttgat
    51481 gttggaataa cagcaaagcc gtcacccatc attttttgtg agaataccgg atcttttact
    51541 tcagataaag gaatcacttc tccagaagct gcggaaacta gttcttgttc tatacctaag
    51601 tattgcttac tacctgcttg tccatttcct gtagaaacca tttctggctc tggaatctct
    51661 gctcctgatt gcagcagatc ttcgatatct gatttcaaga catctgcttt tggtccataa
    51721 actgcttgga ctccattgtc tttgacgatc aatcccatcg cccctgctcg tttccaggct
    51781 tcttcagtcc ctactttttc tcgatcattt acacttacgc gtaaccgagt catacaagca
    51841 tcgacatctt tgatattttc ttttcctccc agcaaatgaa tgatctggac cacttgctga
    51901 tctgctgttc ctgttccgct gttagataca acggtttctt cactgtcatt gtcgtaattt
    51961 ccgttacgtc ccggtgtagc aaagttgaat tttttgatca agaaatttgc taagaaatac
    52021 gtaacaacac caaaagcgat cacacacaac acgaagttga taagatctcc acctagaccc
    52081 gctttgatag ctaacggtgt ccgagtcagc aattcgatat tcccaaatga atggacccgc
    52141 aatggcaaga tatctgccat cgcaaatgca gccccttgga tcactgaata aatgacatat
    52201 aatggcacag cagcaaacat aaacatgaac tccaatggtt ctgttacacc tgttagaaag
    52261 acagccaacg ctgctgaaaa atacattgat ttatatcttg ctttcttatc aggatcgaca
    52321 ttacgataca tcgcaaaagc cattcccatc aagatacctg aagaaccaat catttgtcct
    52381 actttgaaac gagccggcgt ccagttatgc aacacaaact catattgcga catatcccct
    52441 gcacctttaa gattcactaa atcagtggcc caagccaacc ataacggatc ttgcccaaat
    52501 acttgtgtcc ccgcctgtgc gcctgataaa atctcatacg atccgccaag ctgtgtatag
    52561 ttgataggaa tcgtcagcat atgatgcaat ccaaatggca atagtaaacg ttctaatgtt
    52621 ccaaataaaa acggtgctag aatcggtgcg ctttcttgtg attctgcaat ccataaacca
    52681 aaattattga ttccagcctg tacgtttggc cacaccacag caagaatcaa tgccacgatt
    52741 gttgaccata agataacaac aaacggaaca aagcgctttc cattgaaaaa tgacaaagca
    52801 tccggcaatt ttctataatt atagtactta ttataagcca ttgccccgac aaatccagcg
    52861 atgatcccta cgaatactcc catattcaaa gctggtgctt ccaatacact tgtaaaaaat
    52921 cctttaacca tgatcttcgt accgaacaga gtgtgggtaa atgcatcttc attcgcaagc
    52981 atgtcacttg tgacaccaaa gatagcccca gtgatccgat tgattaaaat aaatgagatc
    53041 ccagctgcaa atgctccgcc tgctctttct tttgcccaac ttcctcctat tgctaaagca
    53101 aataaaatat gaagattacc aataattgcc caaccaatat tttcaattac tccgcccgtt
    53161 gtgacaagcg gcgtccaatc aggattgatc aagggaattg atttccctat actgatcatc
    53221 aacccagctg ccggcataac tgctaccaca accattaaag cctttccaaa tttttgccaa
    53281 aactcaaaac taaatatctt tttcattccg tttagctcct ttacgcaatc ggtttcgtta
    53341 aacgtattat agcgtgcgtt ttcttttttg taaatgcttt attttttatt tttctgtcta
    53401 taccattcta taaaacctat aatacgtgtg ttcccttcta taattttctc ctaaaaattt
    53461 atttttttaa gcttttttct atgaaatatt ttcttttgtc tattgacttg tgcaatcggt
    53521 tgcgtttatt atgaacgtat aaaacagctc attaggagga gaaaaatgaa acagatcaaa
    53581 cgactatttg ctattgatcc atggaaaatc agcacgacac aattagataa aaaaaatctt
    53641 cgactgcaag aatctttaac aagtattgga aacggttata tggggatgcg cgggaacttt
    53701 gaagaaactt actcagggga ccaccatcaa ggtacttatt tggcgggtgt ctggtatccc
    53761 gataagacac gtgtcggctg gtggaaaaat ggttatccag aatactttgg aaaagtgatc
    53821 aacgccttga actttatcgc gatagatatt tcgatcaacg atcagctgat cgacttggct
    53881 tctttggaac cagaagactt ttactgggaa ttagatatga aaaatggtgt attatctcgt
    53941 acattcacaa tcactacttc tactaataaa gtacgcattt ccttcgaacg ttttctgagc
    54001 attgtcaaaa aagaatcagc ttatatctat ttaactatag aaatgcttga aggtagcgga
    54061 acagtcaaac tcgtatcaaa attggatgga aatgttcaaa acgaagacag caactatgat
    54121 gaacattttt ggaatgagat tgatcgtgga actatagaag aaatcggcta tttgacgact
    54181 aaaactattc caaacaactt tgggattgaa caatttactg ttactgcagc aatgcgccat
    54241 ttcgttaatg gaaaatcagt catccctgac tatcacgaag atactttagc attacatggt
    54301 tgcttgtctg cagaattatc tcaaggtgag acatggtcag tggaaaaaga aattatcgtt
    54361 cttactagta gagatattcc agaagcgcag caagcagaaa aagctgtctc agaattaacg
    54421 aaccagacaa acaattatat ccaagcaaaa gaagagcaaa cagcggcttg ggcagaacga
    54481 tggaagcttg ccgatgtggt catcgaagga gacgaagaag cacaacaagg tattcgattc
    54541 aatttgttcc aactattctc tacttactac ggcgaagatg aacgattgaa tatcggcccc
    54601 aaaggattca caggagaaaa atacggcgga gccacttact gggatacaga agcgtatgct
    54661 gtccctcttt atcttgcttt agccaaacct gaagtaacta agaatctgct gaaatatcgg
    54721 cataatcaac tgccgcaagc aatccacaac gctcagcaac aaggattaaa aggcgcgctt
    54781 tatcctatgg tcaccttcac tggtgtcgaa tgccataacg agtgggaaat cacattcgag
    54841 gagattcacc gaaatggcgc gattgcctat gcaatctaca attatacgaa ttacaccggc
    54901 gatacggaat atttgaaaaa tgaaggactg gaagttttag cagaaatcgc acgcttctgg
    54961 gcagaccgtg tccatttttc aaaacgagca aataaataca tgatccacgg tgtgaccggc
    55021 ccaaacgaat atgagaacaa tatcaataac aattggtata caaatacgat cgcagcttgg
    55081 gtcttgcgtt acacgcgaga aaattattta acttatcaaa aagaagctgc tatcactatc
    55141 agcgaagaag aactagccaa atggaaagat atcgcagaga acatgtactt cccagttgac
    55201 gaagaattag gaatctttgt ccagcatgac acattcttag ataaagatct catgcctgta
    55261 tccgatattc ccacaagcga tcttcctctg aatcagcatt ggtcttggga caaaatcctt
    55321 agatcttgct tcatcaagca ggcagatgta ctacagggga tttatttctt caatgatact
    55381 tttacaaaag aagaaaagca aaaaaacttt gatttctatg aaccaatgac tgtccatgag
    55441 tcttcccttt cgccaagtat ccattcgatt ttagcagcag aattaggaat ggaaagcaaa
    55501 gctgtagaaa tgtaccagcg aacagctcgc ttggatttgg ataattacaa taacgacaca
    55561 gaagacggct tgcacatcac ttccatgact ggtagctggt tgtcgattgt ccaaggattt
    55621 gctcaaatga aaactgccca tgatcagttg agattcgcac catttctgcc aaaaacttgg
    55681 aaagcttatt ctttccatat caattatcgc ggacgtttat tgttcatctc agtcacagat
    55741 gaacaagtta ctttcgagtt attagaaggt tctcctctag aaatttacgt ctatgaacag
    55801 tcttattctt tagcggatcg tctagaaata acgctaagaa aggaggaagc aaatgtttaa
    55861 aggggtattg tttgatttag acggtgtcat taccgatact gctgaattcc attatcgtgc
    55921 ttggcgaaag cttggtcagg aaatcggcat tacgatcgac cacacattca acgaacagct
    55981 aaaaggcgtc agccgagaag actcactctc tttgctttta gcttatggcg gaaaagaaca
    56041 ctctttttca aaggaagaat ttgcagaatt agcgaaaaga aaaaacgatt attatttaga
    56101 gatgatccag acgatcgaac caaaagatgt cttccccggg gtcgtgcctt tactggattc
    56161 tttaaaagaa gaaaaaatca agattgctct tgcttctgca agtaaaaacg ggccattcct
    56221 acttgaaaaa atgggtctta caccttactt tgatgctatt gccgacccgg cagaagcaac
    56281 aaacggaaaa cctgcgccag atatcttcct ccttgcagca aaagctgtcg gactaaaagc
    56341 agaagaatgt atcgggatcg aagacgcgca agccggtatc caagccatcc tctcaagtgg
    56401 cgctcaacca attggcgtgg gacgtgctga agaccttgga gaggagatcc cgcttgtccc
    56461 tgatacgact tttcttacga tcgattactt gaaagaaaag tggcatgacc atggataatc
    56521 cagagatctc cgtcgtacca gttccagaga ctgaaataaa gaaaatcaca ttgaagaacg
    56581 actacttaga agtcgttctt ctcaattacg gtgcccgcct gcaccaaatc ttcgcaccag
    56641 acaaagaagg aaaatcagaa aatgttttat tgtcttacga tcgtttcgaa gatgttctaa
    56701 cagacaaaag cttttttgga gcaactgtcg gacctgtagc cggaaggatc cgggatgctt
    56761 cttggggtga gcatagattg gagaaaaact gcggatcaca ccatatccac ggtggcacga
    56821 aaggctggtc tttccaattt tgggaagtag aagttttcaa aactccatca tcaataggtg
    56881 tggtctttta cttaaaagat gaattttcta cctatccagg accaatcact gctacgatca
    56941 cttaccgttt aacaaaaaac gaattagaga tgacaacctc tgctagtagc cttgttgaaa
    57001 ccatctgcaa tccgaccaac catgcctact tcaacctttc aggaaatgga acaagagaca
    57061 tctatgaaca tcagttaacg gtctttttag atggtgtgct tgaattagat caagaaaaac
    57121 tgcctactgg aaattggaca aaaaaagaaa aactgccaat cgactttaga aaatccccaa
    57181 cactgcaaga gatccttgca tgttatccag atggactaga tgatgtattt cttttgcatc
    57241 atcctaggct ttccaaaact tccttacaat tattcgagaa gcattctggt cgccaaatga
    57301 ctatagcaac ttctaataaa agtatggtat tattttctac gactggattc gaagctgatt
    57361 tctctgtcaa tggaaaacag atgcattcaa actacggatt agcaatcgaa ccgcaagaaa
    57421 tccctgatat cgttcatttc cccaaatttg gatcgatcaa cctccatcct ggtcaagaac
    57481 ggatcagtca gacgatttat cggttctctg cacaataaca agagaaaggt gtttttatca
    57541 tggctattac cgtaaaagat gtggcgaaaa aagccggtgt tgccacctcg accgtctctc
    57601 gtgtcatcaa cgatcatcct agtatttccg agacaacaaa gaagaaagtc cgaaaaatta
    57661 tggatgaact aggttatgtt cctaacatga ctgcccgcaa tctaggaaaa cgcatttcca
    57721 gtgccatcgg cgtaatctta ccccctcttg attcaaaaga gcggatcggt aacccatttt
    57781 atcttgagat catggaagca atcaacgaag aagcgcggaa ttatgatatg actacagcaa
    57841 tcgctactgc taaaaatttt gaattgctgt tagagaacgt ccagcgtatg catttacaaa
    57901 aacaagtaga tggttttatt ctagtgtatt cagatagtca agatactgtc attgattatt
    57961 tataccaaaa ccacatccct ttcactttga tcggacagcc ttatcatcat gagaacgaaa
    58021 tcgtttatgt agataatgat aaccagcttc taggtaaaca agcaacagaa tttttgatcc
    58081 agaatggaca taagaatatc ctgtttgtga caaacacgac tcatgaaagt ttgtatttcg
    58141 aacgatattt cggttatcaa aaagcaatga tgatggctgg tcttcctgcg ttatccagtg
    58201 tagtactaga gcatccggag gattatctgt cctttgagaa aacgttgaaa gaaaacgaag
    58261 ccacagcatt ggtggtcatt gacgatatct tcgcccttcg cacgatgcag ttggcgcaaa
    58321 tgtatggcta tgtcattcca gagacactct ccgtcattag cttcaacaac tcgatcttct
    58381 caacattaac acacccttac cttacaagta tcgatatcga tatctccgaa ttaggacgtg
    58441 tcgctatgca aaagctgcgc gaattgatcc atgaagaatt gagcagtggc gtacgtttgg
    58501 tcatccctca tcgcttgatc aaaagagaga cagtcgttcc tttagaaaac ccatactaaa
    58561 tagctgactc actaaatacg ctgaccattt atcctatggt cagcttttta tgtaaaagaa
    58621 gatactactt ctaacacatt gtttatctgt aaaaaagagc gtgggacaaa agtaaagaac
    58681 acttttgtct cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcggaa
    58741 ataagctgaa attcacaaaa atttaaaaag caattttctt gagtttcttc ttatttcttg
    58801 gagttaaacg cttctgtccc agcctctttt ttccttcctt caatttttta cacgtcgacg
    58861 attcaataaa aaaccgccta atattgtaaa gacaggtgca agaatcgaga aaaaggcgaa
    58921 tggcaagtaa tgcaacgcgc taactttcat cgttcccata atgaatgtgc cgctgactcc
    58981 ccacggtacg agagaattga cggtcgcacc tgaatccgct agtgttcgtg tcaaataggt
    59041 ttttgataag ccaacgtcat caaaagaacg cttgaatgtt tctcctggaa gaatgatgga
    59101 cagatactgt tctccaacga tcagattgat tccgatacag ctcaaggcag taaacccgac
    59161 taaacgagaa ggtcggtcca tcttttcttt taattcttga acgatggtat caacgatcag
    59221 atatttgatc agtaacccac ctaatgccaa tgcaagtaaa atcaaactag cagaacctaa
    59281 catactcatg atcccgcctc gagaaagcag ttcatctagc tttggatcag gtgtatttgc
    59341 gacataacca ttcatcaaga tgtctgctgc ttttccgacc gtcaaacttt tctcatggat
    59401 aaaagaaaga acgagagcag ctgtcgatcc cgctagtaat gtagggatag ccggaatttt
    59461 tttgattgcc cccccaaata aaataactag tggaatcaat gtccaaggag agatccaaaa
    59521 gctcgctttc atgttttgga tcatttcatt gatactagct agatcggctg aatgactgtt
    59581 atgtcctaat atcgtataaa aaatcaacga aaggacgaat gctgggaaat ccgtataacg
    59641 cataccagcg atatgttcga atagattaac tcctccaata cccgctgcca agttggtcgt
    59701 atctgataaa ggcgagcagt tattccctaa aaaggcaccg gaaacgactg cgcctgtagt
    59761 cattgcatca ttgaatccaa gtaaatgacc gatccccaaa aaagcaattc caatcgtcga
    59821 aatcgttgta aatgaactgc cgacagtcac tccgacaatt gcacaaacaa gaaatgctgt
    59881 cggtaaaaag aaacgcacag aaacaagttt aaatccataa accataatcg tagggatggt
    59941 tccagataaa atccatgaag caactaagac accaatcaaa agaaaaatca ggattggaat
    60001 gatccctgga gtgattcctt tgatgatccc gtcatggatc tcatcccatg tgaacttttt
    60061 aaatctgcca taaaacaaca aacacatgaa agctgcaagg atcgggatat gtggcgagag
    60121 ctggaatccg atgatcagta ctcctaggag tgccaacaac acgatcaata cagcgatact
    60181 ttcttttaaa gaaatatttt tattcatcgt cgatctccta tttcaaatga aaaggccctg
    60241 ccatgaaaaa actattcact tggcaggacc tcctactaaa tcagcgacaa aacgtcggct
    60301 gaaagactta tttttttatt tttcttctaa acttttttct acctcaatcc cccaagaatc
    60361 tttcttgaag tgattgatga tcaatggaat aacgaacatg accactgtga tagctgctac
    60421 taataatgca tcactcaatg tcgctgtacc taatgtagca aatacgacaa cgatgataga
    60481 gaagatcagc atgcagctga ctacccaagc taatccgttt ccgtttttac caattcggta
    60541 aggacgttcg aattctggat gtttcttacg cattttaagc aatgaaacag caatcaaaat
    60601 gtacacgata caataaataa cagttgtagc atttgtaagt gtcaagaata cagcacttac
    60661 gtcatccatt acaccataaa gcaaagcaaa taatgaaata acgattgatt gtgttagaac
    60721 gacatttcta gatacgccgt atttgttttc tttatggaat ccgaattttg gtggaaggaa
    60781 tccttcacga gctacttgga tgattgtttt tgatggtcca gtaacccaag ctgataattg
    60841 taaaagcaca ccaataaata ccatgaagct gaagatattt ccgatcactt ctggtaagcc
    60901 taggatttga cagtcaatca agataggctg cgtgatattt gctaattcca tttttccatc
    60961 tggtacgaca tttgctacaa gcatcgcatt gatgacattc aataaaacta atccgatcaa
    61021 agcgataaat acgccttttg tataattttt tgttgcgtct ttcaaacgag gcatgtaaac
    61081 agaactcatt tcgatcccaa caaaaataaa tgtgatacca gctaagtatt tcaatgaatc
    61141 aatatgttcc aagtcaggga aagcttttga ccatgaaaag tcacctagat agccgtttga
    61201 gactagtccg actttgaagg ctgccaaaac acctaaaacg aacatgatga ctactgggat
    61261 atatacccct agccatacac cgatgttccc gccgactttt gccatatcga atttcaagtt
    61321 caagatcgtg atgatccagt agataactaa aatacaacct aaaacgaacc agtgattatt
    61381 gccaagttct acattaccaa atgtgtttcc taacaatggt cctaaagtag aagcgaccat
    61441 taccatacct gggaacattt gcacccataa tagccatgca acgacaaatc cccatttgct
    61501 gcccagtgct gtttttaccc ataactgagg accgccttct tgaggaagca tcgtggaaag
    61561 ctcaccggaa accattgata tcggaccggc aaaaaacaaa actgcaaaaa ttgaataaaa
    61621 aatcaatgtc caaccgactg cggctaacgt aggaatactc cgaaccgttg cacacagtgc
    61681 cattgtcaga ccgataaagg tcccaagacc cattttactt acttcagatt tactcaccgt
    61741 tgacactctc cttgattttt tggataaaat aacggtgtct ttgaagtagt ttcagcaata
    61801 aacgaaacaa ttcagcgaat atttgagggg attctttgag tttattgctt gaagggtttc
    61861 ttcaaagaca acctcaaaac aaagggatcg aagagcttag gtctcccctt gctcttcaga
    61921 tccaaacatg cgtcagaaac agttagtatt caagctgttt tctaacatga ttaccttgct
    61981 tattttgctt cgcttgccaa taattggtct gcgtagattt tttccaattt ttcttgaaga
    62041 gctgctttga ttttttcagc atattcttca aagtgttctt ctttgttcat gtatggtgtc
    62101 attacgctgg cacgcaatac agttactttt ccggcacggt tccattcttc atctgagaat
    62161 cctagttggt tcacaaattg taatgggctg ttgccatagt ctgggatagc aaagtcagta
    62221 tgtgaagtta agaactcatt accgtaaatg cttcctttca cgtatgaaga ataatcatat
    62281 acgtcatggt tcaatttatt cattgcaact aagtcatcgt tgcctttttc tttgaataca
    62341 tagtctacca tattgaaatc tggataagtt aatggatgaa cttcgatttc tttgtctcct
    62401 actttaaatg acaagtcatt caagaagttg tagaaacgat gtgcaccttc gattgatgca
    62461 cccattaatt taccatatcc tgtgacattc aaaggtaaga catggtgtgc tgcccaaacg
    62521 ctggctgctg ttgcccctgc ttttgaacct tccaagatat aagccccaag taatgctggg
    62581 atatctgcgc ctttttcaaa tacatatgtc gcaaagtaag agataacgtc tctcatgcgg
    62641 atatctttga tgacgatacc accggcagaa tatggaacat agcccatttt atgtgggtca
    62701 atcgttacag attctgcttc ttcgattgct ttatatgcac tgtgtacttc ttctaagata
    62761 taatctttgt tttctgtaaa gacattgtat ttgtaatgaa catcttttaa atcttcaaat
    62821 ggaatgaagt tgttgtcttc gtctaagaaa atcgcacggc cgtatccgcc ataagcagca
    62881 tctacgtgta agtagaagta aataccgtct ttttctaata cgcgacgcaa tgcaacgatt
    62941 ttatcgatac cgtcgattgc tccttcttct gttgatccta caacaccgac tacacctagg
    63001 attggtgttt tttcagctgc tagaccacga acgatttttt caagttcgtt gatgtccatg
    63061 cgatagttgt ggtcaactgg cactggaatc acttggtcta aaccgatacc gatgatgtcg
    63121 gcagctttta gccatgaata gtgtttagtt tgtggaacca accattttcc taatttttct
    63181 aaatgttttc cgctacgtgc tgagtgagct ttgatttcgt cgattttttc tgggacgctg
    63241 tctaacagat tcatgatttc ttctgttgac aagttcatta attcccaatc gctttttcca
    63301 gcaactagtt caggtgtcac ttctttcatt gcaagcggca atgatttgat gttgcgggca
    63361 taccaaagtc cttctaagtt tgctaaagaa ccatcagcaa cgatgtggcc ccagccatct
    63421 ttatagctca ttaattttgc aaattccatt cctacttctt cttccatttg gcttgttgca
    63481 ggagaagatt cataggcaac gttattacca ttccatagca tagcaaaatt gtacgctagt
    63541 aaagatggca tcaatgtttc agagttcata tgaccccagt aacgtccggc attatgccaa
    63601 ggaacggaat gggtacgcat ccgcgctgaa atttctgata gaacatcctt tgttttattc
    63661 acagtgtgtt caaaactagc actgcttttt tcttctggcg taataatagg catgtcttga
    63721 ggcatgtagt tttgacgcca gcctaaatgt tcatccacca attcattcaa caaagcttta
    63781 tagatttggc cgttctcagc tttgtcccca atgaataggg catttaggtt tagatctttc
    63841 gacaatgatt cactcatagt gaaaccctcc taaaaaaata tttaaatttt gtcgagccct
    63901 acattacacc tgttcttcta ttctgtaaac taagttttgg cctgattttt aatcatattt
    63961 taatatgtat caattatccg aattgttcac acttttgaaa taattaaaaa aaacgaggga
    64021 atttacaaat gtaaactccc tcgcaaagat tgtattaacg acaaccagcg ttctatttta
    64081 ggatgggttt caaaattgga atgttgtaat agtatttccc ttttttaagg taaaaactta
    64141 ctaaattcta atcaattact ttggccaaaa agtaattttt cttacctttt cgaataacca
    64201 caaacttacc atcgaattca tcggatggat ttatgacaaa atttaaatcg gtaacgcggt
    64261 caccgttaat acttatagct ccgtttgaaa catcttcacg tgcttgacgt ttagatggtt
    64321 caatttttgt ggaaacaagc agttccacga tattttcagg tgtgcttgaa atttcaacgt
    64381 tcggcatttt accgaatcct tgcgcgattt cttccgcatt tagttcttta atatttccag
    64441 agaacaatgc ttgcgttatt ttttgcgctt ctttcaagtc ttcttcacta tgcacaaatc
    64501 ttgtaacttc ttctgccaaa cggcgttgtg cttcacgttt ctcaggttct gtctctactt
    64561 ttttcgctaa gtcttcgatt tcttcttgag acaagaaagt aaagaatttc aagtatttca
    64621 ctacatcgcg atcatcttga ttcaaccaga attggtagaa ctcaaatggt gaagttttct
    64681 tcggatcaag ccaaacggca ccgcctgcag tttttccgaa tttcgttccg tcagccttca
    64741 gcatcaatgg gatcgttaaa ccgaacgctt ctgcttctgg tccttctaat tttcggatta
    64801 ggtctaatcc agctgtgata tttccccatt ggtctgctcc accgatttgc aattgcacat
    64861 cgtatgtttt atgcagatgc aaaaagtcga tagattgcag gatttggtaa gtaaactctg
    64921 taaatgagat tcctacttcc aaacggctgg caacgatatc ttttgctaac atcgtgttga
    64981 tgttgaagtt tttaccgtag tctctcaaaa attcaagtaa agagattttt gacaaccagt
    65041 cgtagttgtt cacaaatgtg atatttgcat ctttaccaaa caattttctc atttgattgg
    65101 aaagtgcgtc cacgttatgt tgcacttgtt ccatcgtttg caacacacgt tctgttttgc
    65161 ggccgcttgg gtcgccgatc gttcctgttc cgccgccaat cagaatatat ggatgatggc
    65221 ctgccaattg gaatcttttc atcatcataa atggaatcaa atgtccgata tgcatcgaat
    65281 caccagttgg atcaacgccg caataaagtg aaatgctctt ttcttcaacg agctcgcgaa
    65341 gtctttcttc gtttgtttgc tgattaatgg cgtcgcgcca agttaattca tcaataatat
    65401 tcattttact gacctccgtg tagttttttt atgaaagcag gaacctgcta gcatatgtaa
    65461 ttaattattt cttcaggata ggataggaaa taaaaaaagt cccttggcat ataagccaag
    65521 ggacgaaatc tcgcggtacc acccaatttg tatgtctaga tatccaacat acctctcgaa
    65581 catttaacgc tgtcgcgtgg caatcactgc caaagactcc tgaaatgtaa ttcgctttaa
    65641 agcatgtact ggtttgcagc gaccaccagc tttctgaaac attggcttta acaactactt
    65701 cgtttcgatc ttcgtcttgt tattattaat atgtgtctat catagagcgc aactttttct
    65761 ttgtcaacag aaaaaggata ttttttttaa attcctcatt ttttcttttt tgaatccgct
    65821 ctaattccga acattttatt ttcaattatt gttaaaagaa ataacttttt ctgtattttt
    65881 tctgcaattt atcgagaagt cactttactt tgcctcttat tctttcttcg ttacaataag
    65941 gaatgggaca agtaaaaaaa tgaaaaagca agggacgagg tatatggaaa aaaagcaaaa
    66001 aattatttta ggagccactt ctgctttaac tatcgttatt ttaattctaa gtttgttttt
    66061 tgtcatccga tcatctacaa aaaaagaacc aatcattcct gaaaaaaaaa taacagaaaa
    66121 agcaaaagaa ccgacagatg ataagcaaca actagttact caagcaaaaa atctggctca
    66181 aagctacgat tatgaccaag cgattgcgtt attggaaaaa gatagtaaga acggatctac
    66241 tgaaacacaa cagcttcttc aaacactaaa aaaggagaaa gaacaactga tcgactggtc
    66301 tgatcctact cagatctctc atgtcttttt ccacagcttg atcgtggacc cgcaaaaagc
    66361 cttccactct caacaggcac aaggatacaa agattatatg gtgactgttg aagaattcaa
    66421 ccgttcgatc gaacaacttt atcaaaatga ttttgtttta gttaatttga atgatttgat
    66481 ccaaaaagac gaacaaggaa atctttcctt taacgggctt gcgctacctg aaggaaaaaa
    66541 accattggtt ctttcacaag atgatgtcag ctattatgaa tatatggata attccggttt
    66601 tcctagccga ttggtcgtat caaataacaa tgaaatcaaa aatgtctata aagagaaaga
    66661 taaagaagaa atcggtgact ttgacatggt tcctctgatc gatgcctttg tcaagaaaca
    66721 tcctgatttt tcttatcgag gcgcaaaagg taccttggcc ctgacaggtt acaacggagt
    66781 tttaggttat cgaacatcaa aatctcaata tggagacaat gaaaaaacaa aaaaagagat
    66841 taaagaagct aaaaaagtgg cggaacagtt gaagaaagac ggctggagct ttgcttccca
    66901 tacatggggt cacttgaata tgactcaagc ttcactagaa gatgtaaaaa aagacaatga
    66961 attgtggcag caagaagttg cacctatcct aggaaaaaca aatattctga tttatccatt
    67021 tggtgcagac atcagcgact ggcagcctta ttcagcagaa aatcaaaaat ttgcttactt
    67081 gaagagtcaa ggtttcgata ttttctgtaa tgtcgatgct tctacaccag cttggggaca
    67141 attaggtacg gatttctatc gaaatgctcg gatcaatatc gatggcattc gttttcaatc
    67201 tgatttagac ggaaaaaatc ctattcttga tccattcatc aatgtccatg atgtctatga
    67261 caaagaagca cgcgaatctg cttgatttta cgtcatttat gaaccaaaaa agccgaactg
    67321 acattttatc gtcagtttcg gcttttttat gctttatttc ttcttcggtg cgattccctt
    67381 gatcaggact tctgtccatt ctgctgtata tggaagcagt tttgcgtata tctccggctt
    67441 agaaattcct tctaccattg cttgaaaata gaggatcaag aactcatccg aatatttcaa
    67501 atccactttt ccttctttcc gccctcgatg gaaaagatca agcatgatac tgaaactttc
    67561 ttgcgtatac gcatccatcg cgtcagaaaa ttcttttttt tctttttttg tatagtatgt
    67621 catcaaatcg atataaaatt gtttgttcac tcgattgaga taacggattt tgttttcact
    67681 catggcaatc agtgtttctt caaatggaag attttccgca atcaacgctt tcgcttcttg
    67741 ccctaaatca ttcataaatg caaaaaatac ttcataaatc agcccttcct tgctgccaaa
    67801 atatttaaaa atcgtcgctt tacttacctg agcgttctca gctaccttat ccattgtcac
    67861 tccagcggtt cctatgtctg tgttcatcaa gtcaaaagct gcttgtaaaa tctctttctt
    67921 tttctcttgt gctctctttt caaaaccgtt catttgtaga gcctcccgta attttaaact
    67981 tttataatat gtatagttca aatttattct gaggtttatt ataactatct attgagaaag
    68041 tatcaagcta gcaattcctt gtttatgaat tcattgacag aaatttataa aaagaatatt
    68101 atgtactaaa ataataaaaa aagttttata aaaaggtata aaaccttttt attaaactat
    68161 ttaatcttgt ttagtatata tttttagaaa aaggagtggg gtttttatgg cagaaatcgt
    68221 caaagtcaca catttgaaaa agagctttgg tcgtttcgaa gctttaaaag atgtctcttt
    68281 caccgtacat tcaggtgaag tcgttggatt tatcggacca aatggttctg gaaaatccac
    68341 gacgattcgg acacttttag ggattataaa aaaagacggc ggaacggcaa cgatttttgg
    68401 gcaagatgta tgggaagata gtttatctat ccatgaacgt ctttcttatg ttccaggcga
    68461 tatctctttg tggggaaatt tgactggagg agcgatcatc gatctcttca tgaagcttca
    68521 tggaaaaggg agtgctcaaa aaaaagatga actgattcaa cgtttctctc ttgatcctaa
    68581 gaaaaaagca aaaaactatt caaaaggaaa tcgccaaaaa gtgggcttga ttgctgcttt
    68641 ggctgttgat tcggatctct atttatttga tgagcctact tctggtttag atcctttgat
    68701 ggaaacgatt ttccaagaag aagttgaaaa attgaaagaa cagggcaaat ccatcctact
    68761 ttcttctcat attctcagtg aagtcgagcg actagcggat aaagtcgtga tcatacgcga
    68821 aggaacactc gatgaagaag gaacactcga tgaattgcgt catttgactc gttcgaccat
    68881 cactatagaa acagaagcag agaatagtca cttagcacaa ttgaacggtg tccatgactt
    68941 tgtgcaaaaa ggtcacctcg ctcaatttgc tgctgataac gaagctttcg atgtgatttt
    69001 agctgaagcg tccaaattag gtgtgaaaaa atttgaatct attccaccga ctcttgagga
    69061 tttattcatg agacattatg aaggatgagg aggaagcgaa atgactgata aattttcacg
    69121 ctggtccacc cttttttctc aaaaccttag ccgtgactgg aaaaagatcc tgatatggct
    69181 agtcggtgtc actgcttttt ctggtggttt tgtccctgcg tttgaagaat tgacttccgg
    69241 acaaggatta gctggattat acgaaacctt gcaaaacccg gcaatgattg caatggtcgg
    69301 accaactcca gcttcgaatg ctgctgagta tactttagga gcgatgtatg caaatgaaat
    69361 gttgcttttt tgcagtttgt ttgctgctgt gatctctgtt ttacacgtgg tgagccaaac
    69421 gagaaaaaaa gaagaacttg gtctgattga acttattcgt tcctttcgag tcggacgtca
    69481 agcaaactct ttagctgtca tcgctgaaag tattctgatc aatttgcttt tagcaatatc
    69541 gatcagctgt acgatgattt ttttccaagc ggattcgatc actgcagaag gtgcttggct
    69601 ttttgggcta tctgtaggta gtatggggat tttggggagt gccgttgcac tgtttgctgc
    69661 acaactgatg ccaagttcag ccagttcgaa tggtctgtct ttagcgtttt taggtggact
    69721 ttaccttcta cgagcaataa cagatgtagc tgattcctcc ctttcgatgt ggaatccttt
    69781 aggctggtct tatttgacct atccatttac aactaacaac tggattcctt tgctttattt
    69841 gtttttgttc agtctaatct tgataatcag ttcatttgtt ttagaggaac atcgagattt
    69901 gaacaccggc tatatccccg aacgtgaagg aaaaccgtat gctaaacgtt ctcttttatc
    69961 cttgcgtggt ttattattct atctaaataa agggatgaca atcagttggc ttatcacttt
    70021 tggtttgatg ggggcggcat acggctctat ctacgggaat atgcaacagt ttttagaaag
    70081 taacgactta atgaaacaga tgtttatcca atctggcgtc tcaattgaaa aaaactttac
    70141 aagcacgatc atggtagtct tgatttgtct agctgctatt ttaccaattg ctagcatcaa
    70201 tcgtttattt acagaagaaa cccacggacg tatgaatcag ctgttcgcaa caaaaatttc
    70261 taggaaccaa ctttattgga ccaatatgtt actagctatt ctatttgcta tattggcaat
    70321 cctgattagt tctggtgcac tagggctttc tgctttggct tcgatgaagg atgcgaaaga
    70381 catggatcta ttgatgtttc ttaaagccgg gttcaatttc ctgcctgccg ttttagtgtt
    70441 tatcggaatt agtggctttc tattcggatg gcttcctcga atcggtaaac ttgcttatat
    70501 ctacttagct tactcctttg cactcaatta ctttggcgga attttggatt taccagaatg
    70561 gttttccaaa acagctgtcc ctagctgggt tccacggatg ccaaacgatt cttttgatct
    70621 ttccgtgttc cttacgttat catgtatcag ccttgcactg ttcgtccttg gttctattgg
    70681 ttataggcga agagacttac tagaaggata ataaaaaagc atgtacaatt tcatgctagc
    70741 ctccaaatct gggtcaaaaa tccaacattt ggaggtcatt tttttattta caagtcttct
    70801 aactgctctt tatcaatgat ccagacttct cgattctttc tttttacata accattctcc
    70861 tctaataatt taaattttct agataaagtt tcgggggtcg taccgataaa tgcagcaagt
    70921 tctttcattt tcattggcag gaaaaagtga tccttccctg tcgctttagc taagtcaagc
    70981 agatagacag cgactcgttc ttctactttt tccatcatca aaaaagttgt ctgctgctgt
    71041 acttttgtta atttctcaac gttgattgct aaaagtttca gactaagggc gggataatgc
    71101 agtaagagtt ccgagaaatc tttttgtctc aatatgcaaa tttccgtttt ttctagtgct
    71161 tcgccaaata aagactcatt tggaacgcct agcaactggc tctctccctc atatccgccc
    71221 ggctctatta ctcgtaaaag ctgttccttt ccgctagcag ataattgata cactttcata
    71281 ctgccggagg ccacgatcac taattgaggt tctccattcg gcgcaagaat ctgttctcct
    71341 ttttccacat agtgatgttt gacaagtgcg tgtacttttt cttgatcttg ctgtactaag
    71401 tgattgaaca aaggaacaaa tgaaacacat aaatgatcat gactcataat ctctcctcct
    71461 tctaaacagc tatttctaat aaagaaacat aacttccctt attccaactg ataattttct
    71521 tttatcatag cataaacgtc aagcgaacca gataccagca aatagagtgg atttcttttt
    71581 gtcccttgct ttattttcct tcttttgaga ccttaagtaa ttgattccta tcaatttttt
    71641 aaatatttac tttttgaata atcaaaaaca aaccttcact cgcaagccga gagtaaggtt
    71701 tgtttttcgc attgcttatt tcgtgttgtc tattgaaatt tttccgcacc tagcttttga
    71761 tatagtggtg ctagttcttg taaggtttca gcggttattt gctcaacgtg ttcgatttct
    71821 tgcggataga agaatagttt acccatttga aaggttcggt ctctcatcgc ttttttcaac
    71881 tcttcttgtt tctttgtatc ggacaaatag tcagacaaag ctaagtggtc gtcaagttca
    71941 tgttctggtt gcggccatat gtaataatcc ttgatatcga ctgttttgct ccactctttc
    72001 agtatcgcaa gcaactggtt atacgctgca atgctaccat atcgttcttc gcttttataa
    72061 tgttggtaca tcaaatagat ttggtattgt ttttgattca acaaaactgc tagacaggca
    72121 ttctcgtctt gacgagattc gcttcggtac gcgctccaaa aatgactgcg taaattccag
    72181 ccattcgtcc agctttcgat tttaggtttg ctcatgccgt acatatcagg caaaagtgca
    72241 gctgtctgga gctgaatctc tttccatttt tgccagtgtt ctttatattc tttcttgatt
    72301 tgttcaatct tatctgttgg atggctctct ttgagctgtt tgaatgcgta gattggtcga
    72361 tcaaacaact gataatctga ttttccaagt tccattgttt cgcctctttc cgttatcttt
    72421 tgtattctac ctgaattttg cgatatggac aaatagaggt tgtgaaaaag ctgttctgca
    72481 tcaagtcata agaacagcca aacaaaaaac tgcgatgagc atcaccgcag ttttattgaa
    72541 cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta gaagcagctc
    72601 ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct tttttagcta
    72661 ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa ttccctgcct
    72721 tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca gcttctgcct
    72781 ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat ttttcttgag
    72841 aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca gatccgtata
    72901 acggaccaga agccccaccg actttcgata tcatcgccat agagaatact ttgaatgtat
    72961 cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa acccctcttc
    73021 ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg agatattgtt
    73081 tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct tgaacggtta
    73141 gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc tgtacattgc
    73201 tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga gaaagacctt
    73261 gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg acgcctcttt
    73321 cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa ggcgtcccgc
    73381 ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca tctaaaatct
    73441 tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga cgatagcctg
    73501 gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg aatcctggtt
    73561 tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta gttgcctgta
    73621 cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt gctgcatcgc
    73681 ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca gtatacgtac
    73741 tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata tcgtccatag
    73801 cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc ttaacgatca
    73861 gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga tctggagttg
    73921 gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct acaaacccgg
    73981 catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt tccgctttct
    74041 gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga tagcttttta
    74101 ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt tttttcaatt
    74161 tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg agtatcatct
    74221 gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc atccaccaga
    74281 tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga gttcaatacc
    74341 gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc attgactgct
    74401 tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag agaaaaaatt
    74461 tgcacctctt cggatttttg catctgctca atcatttctt taataccatc tgttactttt
    74521 tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc ttttacatag
    74581 ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat aaatttctaa
    74641 ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta aaagagagga
    74701 gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta gtagcttata
    74761 aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac ttggaggcgg
    74821 ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta atgggtgcaa
    74881 gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct gatgcgatca
    74941 ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg atggtcacat
    75001 gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat aactaaagag
    75061 agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc gaggtactct
    75121 accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc tgctgcgact
    75181 aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt tgtttttggc
    75241 aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc tgtcgtgctt
    75301 ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg ttggaaacta
    75361 gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc ttcgctgatc
    75421 tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact tagctttcca
    75481 gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg ttggatcttc
    75541 ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag ctttttctca
    75601 agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg atcgattgct
    75661 gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag ttcttttctt
    75721 ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact atttattgat
    75781 gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt ggaagtcatt
    75841 tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga aggttctgtt
    75901 tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat actggaaaca
    75961 gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat ccttactact
    76021 ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac acaacaggct
    76081 gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt ttccatgtcc
    76141 cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg atcgttatcg
    76201 cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat ttcaattttc
    76261 ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg accatttcaa
    76321 agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag tttccttgat
    76381 atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag aatattgacc
    76441 agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt ttaaaaggat
    76501 ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt gcccaacgtt
    76561 ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag ataatctcag
    76621 gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg atgtcttctg
    76681 tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga tacatcgtat
    76741 ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt ttaatgatgc
    76801 ccacgatact tgagaattca accgtttgag attttgcaat caatttggta cgattttgga
    76861 catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg aaataattca
    76921 catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt aaaatcaatt
    76981 tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta tttttttcga
    77041 tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt tctggtgttt
    77101 ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc aatttcgtca
    77161 tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc accatgatcg
    77221 tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta tgtttgtata
    77281 aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc ggataagaaa
    77341 cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct gtatgggtga
    77401 ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga atgtcgtatc
    77461 tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt cttttgttcc
    77521 aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt tttcatcttc
    77581 atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc tagtcaatac
    77641 aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta agaaatacag
    77701 cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt ctggtctcgt
    77761 taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta aaagagccac
    77821 taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat ctttcaccaa
    77881 aattgatgca acttcttctc ttgttacttt tttcatatcc tcacctcacg tttttttgtt
    77941 acgacgtagg agtttaccat acatttcatt tcttttcaca taaagtgttc ctaaattttt
    78001 cctaagaaaa agaacaaaac atttctcatc agcaaatgaa aaatattttg ttctttttat
    78061 cagttgttct ttttattttt tatctcttaa ttatgttgct ctttccaata cgacaatgct
    78121 tttcattcct ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct catctccatc
    78181 gttttcaaat ggatataaga ttacatgatc aaattccagt ccttttgcta gactcaaagg
    78241 aatgatttgg cagttttcta atccatatga atttttttta atcgattctt gtagcgtttg
    78301 ggtttcctct tctgtttggg tgataactgc cgctttttct ccttttagag aaaccaaaat
    78361 ctcagctagt attttcaaca attcttcaga ggatcgaaaa gagcggtatt cgggttcttt
    78421 tccttgcggt cggatcgata cgatgtcgat cttctcttga tccacagcat aggttttgaa
    78481 aagctctgtg attgctccgc tagaacggta actatttcta agatcatacc gatggatcgg
    78541 cagatggtag tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag aattaaagat
    78601 tgcctgattc tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt cgatcaatag
    78661 acctaattgt gccagggtat aatcttgtac ttcatcaatc aatagatagc gaaatgctgg
    78721 tacatttacc ttttcaacaa gtaaatgacg aatagctaat ataatgactg cttcatcaac
    78781 agtacgtgtg ggttgccaag cataagctga gccatatcgt ttttcataaa ttctttcaaa
    78841 caactgatgc tcatccaccc atgccatttc ttcgatttgt ctggttattt ttcgatattt
    78901 tttttgtaaa agtttccttg cataagctac aatactttgt tccgaatcat cagaaatcaa
    78961 tttaccgaat aattccaact gcctatcttc tgtcaacgag agtacctgat tctgcacagc
    79021 aggacttttt gcttgtttca aaagatgatt cttccattcg ctcatcagtg cctttttcgt
    79081 tgcttgaagc ctatcgatca atttaggatg agcaggaact ttctcatata ttttttcgat
    79141 cgttttttta gaaaggactg tttttccatt tttagtaagt ccacggataa aattcggatg
    79201 atctaaaaac aaaggatctg attgttttag attatcgata aactttttgc tacgcaaacg
    79261 ttctgtctgc tccgatacat tttctcccgt gatacgtttg aaataagcct cttcaccttc
    79321 tatttcctct gctgaaagct gtgaaaccag ctgtatgatg gttagattca gtggatttct
    79381 ttcacctaat gatggtagga cattcgaaat atagtcgata aaccgatgat taggtgatag
    79441 aatcagcaag tcatcggatg tcatcgtatc tcgatataaa tagagcagat acgcaatacg
    79501 ctgcatgata gcagaggttt taccacttcc tgctacgcca tttaccaaga tattttgact
    79561 tttcacatcc cgaatgattg tgttttgttc cgattggatc gtagcagtga tagccttcat
    79621 ttcattggtc tcatcttggc ctaagacttc taataatacg tcatcttgaa tagctacaga
    79681 cgtatcaaaa aacttgacca aacgatctct ttccaaaaga aattgtcgtc tttctttaat
    79741 aaccgtatcg attttatttt gacgaaccgt ataagaagat ggaccaatca tgttattgta
    79801 aaaaagttcg gcaattggtg aacgccaatc ataaacaaga ttttcatctg catcatctgt
    79861 gaaatttgct gtaccgatat aaaaggattc ttttggctca ttatccagaa agtctacaac
    79921 gacttttcca aagtagggac ttttcaataa acgttcgatc tttgctagct gattttctgc
    79981 gtttttctgt ttgatattca tctgatcgat ttctctattt ttcatttcaa tcatcgaaaa
    80041 agtatccagg ttatctaagt aactgtcaaa attcaatcga agctcgccag ccatttcttt
    80101 caacgactgg tttccttcta tttttgccgt atccatcgat tcccgcagtt cgtttttttt
    80161 atcaattagc ttttgataca cgttcttcag atatgcttct tctaactgtt tttgtgtgtt
    80221 gaccatacac attctcctct ttttttaaag tggtggaacc gattagttta cctcttttaa
    80281 gagtcgaatg caagcttttt aacgagtatc atttgttagg atatatcagt tcttcaggtc
    80341 ggacagtttg atataaagaa aatgcaccat caaggttttt tacgtggtag ccagactgct
    80401 tcaagattct ttccgctaga tagctacgta atccgctatg acagctgacg atatattctt
    80461 gttggctgtc taattcattt aatcggtccc gaagttcatt caaagggata tgtttggctt
    80521 ccttgaattt gcccttttcc aattcttctg ctgttcgcac atctaataaa atttttcctt
    80581 ttgctagttc tgtcggcaat tcatgccatt gtacggtttc actaagccct tctgcaatgt
    80641 tcattgctgc atatcctagc atgtttactg gatctttggc tgagccaaat ggcggtgcat
    80701 acgtcaattc taattccggt aaatcgaaga tcgtcaaatg ccctttgatt gctgttgcta
    80761 aaatatcgat ccgtttatcc acaccttttg ccccaacacc ttgtgcacca taaatctcgc
    80821 cagtctctgg atggaagatc aacttcagta aaatatctgt tgcttctgga taatagcttg
    80881 catgatcttt tcctgataca tgaatgactt caaaagacag ttcgttttct ttggctgttc
    80941 tttcacttaa accggtggat gcggcagtca ttccaaacgt tcgtataatt gctgttccaa
    81001 taccgccttg atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt ctaccttgac
    81061 gattggctgg tgaagcaaga gaaataagtg catcttttcc agtgatttgc tgttttacaa
    81121 cgatagcatc ccccaccgca taaatatcag gaatattcgt ttgataacgt tcgtctacta
    81181 ggataccccc gcgtatgcct aattcgattc cagcggtcac tgctagctgg ttatcaggac
    81241 gaacaccaat ggataaaata actaaatcag cagggatggt acgcccatca tccagtcgca
    81301 atcgtttccc gtggtcttca aatccagcga cagcatgaga agtaatcact tctactcctt
    81361 gatgagacaa ttctttttct ataaaagctg ccatttcttc atccagtgtt ggaagaatat
    81421 gtggtgcttg ttcgatcacc atgacttcta aacctcttct ttttaggttt tctgccattt
    81481 ccaatccgat gaatcctgca ccaacgatca cggcacgctt tggctgtttt tctaatgcat
    81541 gcatcacttt atcgatatct ggtacatttc taatagaaaa aacattgtcg gcttctgtta
    81601 tccctggaat cgatggaaca actggttttg caccaggaga taaaaccaat gcatcgtact
    81661 gttcaatgtg tttttgaccc tcatgtacga cttctaccgt tttgttttcg ggaaagatgg
    81721 ctgtcacttc atgatttgga cgcacatcta aacaaaaacg agcggataat gattctggcg
    81781 tttggacaag aagattttcc cgttcactga tttctcccga aagataataa ggcaagccgc
    81841 aatttgcaaa agaaacatac ggtccttttt caaaaacaac gatttctgca tcttccatta
    81901 atcggcgaag ccgtgtagca gcagacattc cacctgctac acctccgaca ataacgattt
    81961 tcatcttatt tccctcctac tgttcgatca aaccattgat tcatgccacc acgtacattc
    82021 actacatcat aaccagattt ctttaattct ttagctgctt gtttactcct catccctgat
    82081 tgacaaatca catagacggt cttttctttg tctccatcat atctgtttat actttgtaat
    82141 ggaacatttt tagctccttt tatatgcccg ccccggtatt ctgcaggtgt tctaacatca
    82201 agtaacgctg gattttccaa aagcttttcc tttaattccg ggactgttat cgattttatt
    82261 gattgaaata gcaaattcat tttctgcacc tcatttacat ttttataaat acctcatggg
    82321 gtatattaaa caggagcttt attaatatgt caaccagaat gactcttcat ttttttcact
    82381 actttttata tttgcataaa ataccgataa ggggtattat ctaattataa aaatagagga
    82441 gaatgtgtta tggagattga taataaaaat atcctgaacc gtttgaaacg tacagaggga
    82501 caaattcgtg gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga tgtcatcact
    82561 caattaagcg ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt tgcagataat
    82621 ctgaaaaatt gctttgagaa acctgatagt aatccagaag aacaagcggc taaactagaa
    82681 caagcgatca agatgattat taaaaagtaa aggaatgatt tcaatgaaag tcgttttcca
    82741 tattgatgaa ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc tgataaaagc
    82801 ttctccagaa acgacgattg tcgtatctgt aaatggcatc gcgattaccg gttatctaga
    82861 ctctgccaat gcagaattct tagatttgca aggagtcgtt tttcacgctt gcgccaatgc
    82921 tatgcgtgct aatcatattt cagaaagttc tttacctgaa caagtaatcg ttgtaccagc
    82981 aggtgtcctt gatttagttg aactgcaatc ccaaggttat gcttatatca agccttagtc
    83041 agtagaggtt gcgacagaag tgatcagctt caagaaattt cggcttattt ttcgaagaag
    83101 ctacttctgg aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat caagtaataa
    83161 gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttgggctta ttaccgcaga
    83221 tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt gacctaagta
    83281 ttagagaaaa aaacgggaaa atagctcctc atattttgcc gttttttgag ctaataccga
    83341 aggtcagctg attgaacacc gtttaaggaa gagattgtga aaaagtcgct tagtttcgag
    83401 tattaagaaa aattttgtga aaatggattt ctacattttt gcataaattt ggcttaatac
    83461 cgagaaactg gcttttgaat accgtttaag gaagaggttg taaaatcagc ggtttgacct
    83521 gagtattaga gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt ttgagctaat
    83581 accgaaggtc agctgattga acaccgttta ccaagaggtt gtgagcctac catttaactc
    83641 cgaatcacaa gaaataattt caaaaacaac ttctcctgtt tttggtttgg ttggcttatt
    83701 atcgagaagt agctactggg agtctattta tcaaaaaaag gaatgcgaca taggtcacac
    83761 ccctttttta ttttctttga aacatctttc ggtactcgac agcatacaag acaaaagatt
    83821 gtaaaagacc gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt ggtaattctg
    83881 ggaaaagcag caaaaggatc atgccaacat agatgacagc agtcgctact ttgccatacc
    83941 acttcgctcc acttaatttg atatttttag atagcccgat taatccagct acaaacataa
    84001 agccttcttt tatcaagaat agaccaaaca acagccaaag ccacggtcga taccatgcca
    84061 tagacaaaat cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc accttcccta
    84121 agtctgttac ctgattatat ctccgagcaa tgaccccgtc tagataatca gacaaacctg
    84181 agacaacaat gaccgctcct gctatataaa atgactgtct cagcacaaaa taaagataaa
    84241 tcggcagcaa aaaaatacgg aatatcgata aaagattcgg aatcgtttgt atttctttac
    84301 gccagtctgt gcttaatttc attttatcgc cctacctcct cagcactctt ttctttttat
    84361 tttagcttac acggggaata aaaccaatga tttcactgtt tttatttttc tttacagcca
    84421 ctgatattat gagctaattt gagataatca cttgctgttt tcatcgttta ctcttctttc
    84481 ttcctgtcgt ttgcgcattt cttttactag tactttatga gctttttttg atcctcgctc
    84541 taggtcacgc tgccgtttac gtgttttata atcgataaac agactgtcta aatcgtaacc
    84601 tgtcgggtac agatcttctg ctgaacgcac cagacgaaca cgctgacgag gcacgtggat
    84661 tatttttttg tctaaatata catcaatcgt atcttcacct atatcttcat aaactaacgc
    84721 ttctttttgg tattcattga caaatatccg atcacctttt gcaaagagct ggcgttcttt
    84781 tttattctcc ttcctatttg taactgctgt cttaggaaga aattcttttt tctgtgtccg
    84841 ataggctttt tccgccaagt aacgctctgc ttgatggatc aatttcatcg acatatccat
    84901 tttatgagca atccacaaag cttggctttc tcctgtttct ccaacccgca actgatattt
    84961 aggacgaagt gcttcccgat cgaaagccat agcagccggc acaaaatctt catgatctcc
    85021 ggcgaatttc ttgatttctc cataatgggt cgtggcaatg atcaatgccc cttgctcata
    85081 catagattcc atgattgcga tcgctagtgc agccccttca tttggctctg ttccactgcc
    85141 aatctcatct aaaagtacca acgtattcct ttttatcttt ttcaaaatag cagcgatgtt
    85201 ttgcatatga ccagaaaatg tactcaacgc attttctaaa ttttggtggt caccgacatc
    85261 gacgaaaatc tcatcaaaga cagcaagctc tgtcccttct tttgccggaa cttgcatacc
    85321 gaacatcgcc atcaaagtca ataaaccgac tgtcttcaac acgactgttt tgcctccggc
    85381 atttgctccg gtgataatca agcctcgata gtctttaccg atttcaacat ccaatggtac
    85441 cgcatgctca ggtaaaaatg gatgtctgcc ttgcttgata atgatatgtt ccgatttatt
    85501 gattctaggt gtgcttccat tgatttctcg gctaaatttt gcccgagcaa agatgatatc
    85561 taacaccgtg atcgtttcga tcaataaatc gatcacttct tcgttttctg cgatagcacc
    85621 agtcaatgcc gccaggactt gatattcttc actgatttct tcagctttta acaactgata
    85681 gtgttcattc aatttagaaa tcacagttgg ttcaataaaa accgtcgttc ttttatttga
    85741 ttcatcgata atcgttccat ctattttatt tttatagctg gctttgatag gaatcgtata
    85801 gtattcgcct ttttgaacaa tcattgcttc ttggatcatt tctttgtttt tcggatgacg
    85861 caagaatttc agtaattttg actggatttc tttttcgatt agttgtaatt gtttgcgtac
    85921 tttacgcaaa ttacgtgacg cgtcgtcact gactttatta ttttttattt tctggtcaat
    85981 caactcttct acatttatca aatcaggaag atgtttgcta taagcaaaca acaagggtgt
    86041 ctggtattga ttcttatcaa aaaactttgt gatcatccga ctgcttctaa gaaaatccgc
    86101 atattcgatc aaatcggttg gctgcagaat caaccctttt ttgacttgtt ctgtcagtgc
    86161 atcgatcctc ggcaacccca taaatggaac atgctgactg ctttctaaga tcaagcgtgc
    86221 ttctcttgtt tcttcttgac gatccaagac agtttgtagg tttgtcgaga ccgtcatatc
    86281 gctgattcgt tttttgctat agtttccaat tgctcttgct tggatttcct ttttgatccg
    86341 atcaaattgt aattgactaa tcatttcgtt gtccatattc tctccttttc ttgaagagat
    86401 acctgtccca aaataaataa gggcctagag ataaataaaa ggatagaaca cagcttaaaa
    86461 aagtcatgtc ctatccttat tagaatacaa agatattgtt ttcctgacag gtaccgtttc
    86521 ataagcacac caaataaact ttgtttattg gctattcgac ttactgatta gataccagct
    86581 acactcacaa aacatacccc attcttctag gtcttattta ttttccactt aactatagca
    86641 agacacattt tggttgtcaa actttagtat gttttataga tttacaccat taatgaatag
    86701 acacctaccg cgataattgc tccgatcatt ggacctgcga ttggtaccca tgaatatgcc
    86761 caatctgaag ttcctttatt agctattggc aataccgcat gtgctaatcg aggaccaaag
    86821 tcccgagcag ggttgattgc atatcctgtt ggccctccta atgaaagacc gatggctaag
    86881 atcaatccac ctacagcgaa tacgttcgtt ccaggtgcaa attcgttttg tccaaaagct
    86941 agcaacccaa gaactaatac aaatgtacca attaattctg tgatcacatt tgctggataa
    87001 ttccgtatag caggacctgt tgcaaacgtt cctaaaatcg cgctttcatc tttcgttgca
    87061 ttccaatgtg gcaaataaga aagccaaaca agaatggccc ctgcaaaagc tcctaaaact
    87121 tgcgcaatga tgaaaggaac gacaagattc caactaattg cccctgtcat cgccatagct
    87181 agagagactg caggattcaa atgagctggg cccatgaatc cagaaatata gacagccatt
    87241 gttacagcga gtccccaacc aaatgcgatg acaatccagc cagaagcctg tgctttactt
    87301 ttgtttaaat tcacagccgc acacacacca tctccaagca aaactaggat cagtgtccca
    87361 ataaactctc ccatgatttg agtcatatca ctgttcattt atttctcccc ctttagttcc
    87421 cgcagatctg attctgcaat caatgcttct aaacgtttag tctgttgcgc cttctcctct
    87481 tctgtccatt caaaatatcc agcaattgca tcgatcacag gttgcttgat ctcatcaagc
    87541 tgatctcgtt caaacaacag atgatttgta cgacgaatga gataatcgcc tggggccaaa
    87601 accatttctt cttctaaacc ataacgtaac cgagctgact cagcgagact caaaccagga
    87661 taaggtgcca tttcttttgc taattcaaag atacgtcggg cattagtccc gtaaaaatca
    87721 gcgatataag tagcatcttc ttcctctaaa ccggctgcta ctccttcttt tgctaattct
    87781 gtcactgttt cttctaattt agttggatcg aagtttccac ctgaaatctg atatttttta
    87841 gagtcgattt ctttcgtctc tattccgtat tcttctttta ataattggcg tatcaatcgc
    87901 aaagctcctt ctgccatctt acggtaatcg gtgattttcc caccagataa agtcagcaaa
    87961 ccatctggtt ctctttctaa agaactacct ctggaaatcg tagaaggtgc tttttcatca
    88021 cggctgtttt ccaaatggtt caacacgtct tctacttcag cacgagaaac tttgttttcc
    88081 ttatattcac ttactgtatc gacaacttta ttgaagctct tgtctgaaat cgatccatta
    88141 tctccaccat tgtaatcaga accagaatta ccaatcaaca gtggacgaag tcctgcccag
    88201 cttgcttcga tatcattcaa tgtgatattt gcttctggat agcgatgatt gatcacatcc
    88261 aatagataat ctacatcttc ttgtgtgact ttcggatccg taaaatctcc ttggtaatcc
    88321 gtatctgtcg taccaaagta agtcttgttt tctcttggaa tagcaaaaac catccgccca
    88381 tcttgctttc ctgtatcgaa gtatgtgggt tgcggtacag gcagtttttt cgcatctaca
    88441 actaaatgga tccctttagt tggacgcatt ttaggagaga ctggacgcgt aaaattcaag
    88501 tttcgtactt tatctaccca aggaccactc gtattgatca ctaattttgc tttgatttcg
    88561 atcacttcat ctgtcagcag gtcacgagct ttgacgccaa cgatttgatc gccatcatat
    88621 aaaaagccaa ccgctttcat tttacttact agataagccc catcttcggc agcctttttg
    88681 atattatcaa tcactaaacg ggcatcattg ttgcggaaat ctagatagac acccgcgcct
    88741 tttagccctt cttttttcaa aaacggttct ctttccaata cttcttctgg tgtcagcgta
    88801 taattctcat atttggttcc tgtcacattt gccagtttgt cataaaggtc cattgctact
    88861 ttgaccgaga acatattgaa ggttgttgct ccttcgccct catagattgg caaaagcatt
    88921 ggatctggtt ttgggatgtg tggggcaata ctttgtacga ctgcacgttc accaactgtg
    88981 tcagctacta cttccacatc aaatgtcttc agataacgaa taccgccatg aacaagtttg
    89041 gtcgagcgag aggaagtccc ttctgcaaaa tcctgcattt cgatcaatcc tgttttgatt
    89101 cctgatgctg ctgactggac tgccacaccg gcaccagtga ttccgccacc gatgatcaac
    89161 agatccaatt cttcttgctg cattttttga atgctatctt gtcttgtctt gtttgaaaac
    89221 atatggattt cctccttatt caaatgcttg ggtagctttt actgcttttt tccaaccgtt
    89281 gtaaagtttt tctcggcgct cttcctccat ttgaacttca aatagcttgc cagcttcata
    89341 aaattcgcgg atctcgtctg tatctttcca ataaccgaca gctaatcctg ctaaaaatgc
    89401 tgcccctagc gccgttgttt ccaagttttc tgctcgttgg atcggtacat tcaagatatc
    89461 tgtttggaat tgcatcagat attcattgtt tgctgcaccg ccatctactt tcaatacagg
    89521 aatcttgatt ccagtgtcat cttgcatcgt atcgatgata tctctcactt gataagcaat
    89581 cgactgtaaa gtagctttaa tgatatcttc ttttgtcgta ccacgtgtca agccaaacat
    89641 agcccctcgt gctgcctgat cccaataagg agcacctagc ccaacgaatg caggaacaac
    89701 ataaacctca tcttcgcttg tagagcgttt cgctgcgtct tctgaatcgc tagctttttg
    89761 caacatttga agtccatctc tcagccattg aatcgaagaa ccagccacaa aaatacttcc
    89821 ttctaaagca tagtaaacct ttccatttat gccgtaacca attgtcgtca ataaattatt
    89881 tttggataat tgcggttttt caccggtatt catgacaata aatgaacctg taccataagt
    89941 attcttgacc atgcctggtt cgaatgccat ttgtccgaat aatgcggctt gctggtctcc
    90001 tgccatccct gaaattggtg cttcgcttcc ataaaaatga taaccttgcg tataaccata
    90061 aacttctgag ttagaagtga cttttggtaa catcactcga ggaatattta gtaaatctaa
    90121 aatctcctga tcccaatcta gctgatggat attgaacaac atcgtccgac tggcattcga
    90181 gtaatcagta acatgtgctt gtccatcggt cagtttccat actaaccaag aatcgatcgt
    90241 tccaaacatc aattcgcctt tttcagctcg ttcttgcgcg ccttctacat gatcaagaat
    90301 ccagcgtatc ttcgttgctg agaaataggc atcgatcact aaccctgttt tttcgtggaa
    90361 aaattcttga tagccttcat tttttaagtt atctgcaatt ccagctgact gtctggattg
    90421 ccaaacaact gcatgataga ttggacgtcc tgtatctttt tcccatatta ctgttgtttc
    90481 acgctggttc gttatgccaa tcccagcaat ctgatttggt ttgatccctg attcgatgaa
    90541 cgctccggca ataactgact gtacagaatt ccagatttca tttgcatcat gttccaccca
    90601 actttcttgc gggaaatatt gcgtgaactc tttttgagaa ctgccgatgt gtcttccttt
    90661 cttgtcaaag ataattgctc gtgagctagt tgtaccttgg tcaatcgcca tgatatacgt
    90721 tgattctgtc attgtgattc cctccattat atgcaaacgc tttctttaac tactcttatc
    90781 atactactgt tttaaaaaaa gaaactgtca aaattttatt ctttttttct ttttttgaca
    90841 gttttttatt atgaagctat tttttaatca attcttttct tttttcagcg tctctattct
    90901 tcttttcaat cgaataatgt cgtaaggtga cgcaaattct gacagataat attcacactg
    90961 ttttttgttt ctctggcaaa atgtgatcac caaatcatat tctttacctt cttcataact
    91021 tccaatctca ataccgccga ttcccctcag ttctctgatc agatattgtt gaaaagcaat
    91081 acgataaatc ggcagacttt ctaaatcgat tccaatcact actggttttg caatataaaa
    91141 atcgatcatc aaaagaacat tggcatactg gatcatcagg taactaagct cactatcttc
    91201 agctcttttc ctcggcaatt gctgcaccgc tgtttcctgt aatttcgcca gtaactgtgt
    91261 taaggaatgc ccgagcattt tctgctgctg ttccaaaagc cgatcacgct catagacttc
    91321 gatctttcca acaaagaaaa accattcatt gttgatctgg gaaatttggt agcctactgc
    91381 cttttcttct ttgattttca aacgacgagg acgataatgc cataacatcg tttcccgaat
    91441 gtaagtatct aaaacagcgg ttggtaactt tttgctccgt gtcaaatcat actgataaaa
    91501 tatttcttcg tctactatcg aaaaagaaac aaaaaagctg taaaacatca ttgtttcgta
    91561 tacattcagt tctgctgctg ttcgactaag atgcatcacg atgattggat cgattgcttg
    91621 ataaagccgg tccgactgat agagtaattt cttttctttc aaagttgcgt acgtactttt
    91681 ttcattcaat agtctttttt tcgtaatccc gagccagcaa gcaatctgtg cttctgctga
    91741 tgccgtaaac gttgtttcta atacacggtc caatcctaga atgaacggtc ttttttctgg
    91801 tgtattttgc aaaaaaagcg gtcgctgatc ttccgggata tattggaata gttcataata
    91861 aaaatatcgt atctgtagct cttctccgta taattgaccg tttttgattt gtaatccaaa
    91921 ttcctctaag agctggttca attctttgat ctttcgaaaa acagaagact cactgatatt
    91981 aaacgctgtc gctaatcgaa cgatggatac ttgctgatgt tctaataaat agacgagcat
    92041 cttaaacttc aagctttcct gcaataaaaa caatagaatt ttttccaaac ttacatcttc
    92101 gtccaacaca agcagcgcct gatactcatt tcgctgtact tctacttttt tttccatcat
    92161 tcggccaatc aattcaatat cttccaaata ttgatcaaac gaggcttttg acagttggac
    92221 atgctctctc aaaccattga tagaggctgt tcccccttgc agaacaaggt ttcgcaaaat
    92281 caggatctgt aactgctctt ttctttctaa taatgcttca atcttcatag gcagtattcg
    92341 ccacctttga ccacaccata tctggcacac ggtattctgg ctggaaaaat agctcatgtt
    92401 cttctaactg ttcaaacgtc catccttctt ctactgcaat tgtcagttgg tcaagaaggt
    92461 ggaacatctc ttttttgctt tggagctgtg ctccaagaat ctttttttca gaatcaaata
    92521 gaaatttgag tttaactttg ctttgattta ttgctgtaag cttgtagtct acgtatcctg
    92581 tttcaatttt ttggggataa aaaatgctct cttcttctgt caaacctacg cttcctaaga
    92641 aatagccaaa ccactgattg ccgactggac gataggttcc ctgatccttt gtctgtgagc
    92701 cactaattgt cttgctgaca tttcgtgctg tacggacagc gttatttact agagaaacat
    92761 acattttttc atttgttgga cgaaaagtta cttggatcgc atcgccaatc gcataaacgt
    92821 ctttttgaga cgtttgcaag tagtcatcta cccagatcgt tccatcatca ttcaatgtta
    92881 attgttcttc ccacatctga ttgtcggggc gagaatgatt agccagcaaa acaagatcat
    92941 agttctcctt tcttttttcc gtctctacaa tagcggtttc attttcggtc aaagacacaa
    93001 cttgttcttc taaaaaaagt gaaagccctt gatttttcat ttcctttact aacggttctg
    93061 tcatttcagg atctagataa cgaaaaagaa ttgactttcg gctttcgtaa agatggattt
    93121 gtttttttct tgatgccaac ccttcagcta tttcgattcc tgcttgtcct gcaccaataa
    93181 ttgcaatttt ctttgcattc atgatctttt gctgaagagc cgttacgttt tgtatctttt
    93241 taactgggtg aataagcgtt gattctgcag aaatatttcg aaacttttga tttgaaccag
    93301 tcgctagaat caaacgatca aaatccaatt tttttcctcc tgctaaaacg actttttttt
    93361 ctttcaatcc tattactgtc ttttctgtgt aaacatggat tcgatagcgg tcagtcagtt
    93421 cttctttcgt gatccaagaa agttgttttt cactggtaac ctttcctttt aatatcaaat
    93481 tgaacccgct gggaatccat cctaacttcg attgcttttc aatcaaatat atttcacttg
    93541 ttggcatcaa ttggcgaaga taaatagccg catgaatccc tgcaaacgac ccgccgacaa
    93601 ttacaatgtt catctgcatt cctctttctt ataaggtgtt ctactgctaa cttctcaatc
    93661 agaaaacaca gtattcataa ctcgctttca accttatgat acatagcgat gcagcagaat
    93721 caaccctctc catcataccg tgttctaggt ctgcttctca ataatgtact agtggaccta
    93781 attgaagagt atcatttctg aagagatgtt tcaaaaaatg tactcttata aaagaaaaaa
    93841 actcgccttt tacgtacctc tgtccaaata ggaaacagaa aagaacgaaa agacgagttt
    93901 tagcgtgtgt aatgtaatag ttagaataga cacatcattt atttttaatt ctttaatgct
    93961 tcaacaactg cttcgcctaa ttgatgagca gattgaggat tttgccctgt aagaagccgc
    94021 ccatcaattt gaaccacttc tgaaaaagct gctccagact gatagtttgc tcctttttct
    94081 ttcaaagcat cttctgctaa gaatggtact tcattcgttg taccatttgc ttcctcttct
    94141 tcattactga acccagtcac tgtacgtcca gctaaaatag attttccaga ctcatctgta
    94201 actggcaaca atcctacaac accgtgacaa acagctgaga ccagcccttc atttcgatag
    94261 atttgtgaag cgactttccc aagatctatt gcttctggaa aatcccacat tgttccgtgg
    94321 ccaccagcat aataaaccgc tgcataatca tttgcatcca catcatcagg acgtaaggta
    94381 tttcccaagg cccgatttct gaaatcttca tcttcataat aggcccaatc gatttcatcc
    94441 atattcaaaa cactttgcgg ttctaaaggt acatatccgc ctttaggact gacaaaatct
    94501 gcatcgatcc cattttttac taatacatca taaaaatgag tcagttcact taaccacaaa
    94561 cctgtaggac gctcgatctt ttcgtacttt tctacatttg ttaatacgat caatactttc
    94621 attttcccat cctctttcta taaaaattgc gttattgata acgtgctttc aagaaattat
    94681 ataatgggct tggcaaataa agctctttat atgcttcttc aaaaggtttc gttacaaatt
    94741 catcctcacg gttttccaat aatttctctg cccagtcagg atcgacgatc atttgctgtc
    94801 caattgctgc aagttctgca tatttcaata cctgctctac atcttcgcga ttgcggatcc
    94861 caccgacacc aactaaaggg atctttcctt gaatcgtgtt atggaccact tcaagaatcg
    94921 atttatcgtt gtatttatca gaacgagcta ttcgatcata catatttaat gaaacatgca
    94981 gataatctag ttttgactca cgtaatcgtt caagaagcca gatcgtgtct tcaaaacgga
    95041 tacctggtgt ttcatattct tctggtgaga aacgataccc tacgatgaat ggttttttag
    95101 catattgttc aaccgctgag aaaaccgttg ttaataattc ttcaataaaa cgataacgat
    95161 tttccaagct accgccccat tgatcctctc gtctattcga atgcggagag aaaaattgtt
    95221 ggatcaaata cgtatttgct ccatgcaact cgattccatc aaatcctgat tcaatcgctc
    95281 tacgtgtggc tttgccaaat gcttggattg tttcaacgat ttcttcttca ctcattgctc
    95341 gtggtgtttc cgcatcagga cgttttgctg caactgcact tgcagataca ggctgttctc
    95401 cagataaagt tgctcgactt gtcattcgtc caccgtggaa aatctgaata atcgcttttg
    95461 cgccttctcc ttggattgct ttcgctagct cacttaatcg aggaatcatt tcatcatgag
    95521 cgatgctgag ttcacctggc cagccttttc cgatatcttg gacatttgca gcaccagtaa
    95581 taacagcacc aagaccttta gaacgttgtt tataataagt gatctcatct tcggtgatca
    95641 ctccattgaa aaagctgagt tgtgtcgtca taggcgacag catcaatcgg ttcttcaatt
    95701 gaactcctga aggtaaagta attgattgat caaattgtga cattgtcatt cctcccataa
    95761 ttcgctctga ttaacctatc gagaatcatc tatcattaaa ccactcgatt agttgtacat
    95821 gcaactaatt tgcttaaaaa attttatttt ttttgtaact tatctgttgc atcattcatt
    95881 gtctgggcta attctttact tgcttcattt cccaaaactg ctttgtatga ctgatgaagt
    95941 tcttcccagc taccatcgac ttccttagca aattggcgtc cttgttctgt aagataaact
    96001 aatgacaagc gaccttcgct aactgttttg acgtatcctt tatattctaa tttttcaaca
    96061 aaacgagcaa tcgttgatgg ggcggaatag agcatttgtg caatatcttt ttgtggtatt
    96121 ccatcttttt cttttaatat caataaaata aaaccataag ttggtgccag acctgtcctt
    96181 ctaaacgctt catcagctaa tttattcaac atgcgatcta gctttttcac gctaaagaaa
    96241 agacattcat ctaacgcttt ttcaattggc ttttccattt catcccctcc attggcgtac
    96301 atatcatagt atgattttta ttttttgtca aaaaacacgg aataatgtcc gcctcctctg
    96361 tctagatttg aaaaaaagaa gtagccatct tcaaagaatt tcggcttgtt ttcgacaaaa
    96421 ctccttctgg aacaccgtga atcagaggtt gtgaaaaagt cgtttagttt tgagtatgaa
    96481 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgaaaa
    96541 actggctttt gaacaccgtt tatatcagag gttgtgaaaa agccgtcctg catcaagtaa
    96601 taagaacagc caaacaaaaa tagttcctca tattttgtcg ttttttgagc taatgccgat
    96661 ggtcagctga ttgtacaccg tttattgaga ggtcgtgagc ctgccgttga actccgagtc
    96721 acaaggagca atttcaaaaa acagcttctc atgttttatg actatggtga cttgtgatcg
    96781 aggagttggc aggagaatac tgtttattcg taaagaaggg gctgtgacaa gacttttgtc
    96841 acagtccctt atttcctttt attataggat ttcgccattt gattggatga cttgtctgta
    96901 ccaatcaaat gattttttct ttaaccgttt ctttgtgcca ttcccttcgt catcttgatc
    96961 aacatagata tatccataac gttttgacat ttgtgaagta gaagcgctga cgatatcaat
    97021 gcagccccac gacgtataac caaataaatc tactccatct ttgatagctt ccttcatctg
    97081 ctcaatatgc tgtttcaaat agtcaatgcg gtaatcgtct tgaacggttc catcttcttc
    97141 tactttgtct atagctccga gtccgttttc agctatgaac agtggttttt gatacctatc
    97201 atataattga ttcaaagcaa ttctcaaacc cttaggatca atctgccagc cccattcact
    97261 tgatggaaga tacggatttt taattcctcc agttattaag tttccttgga ccccttccca
    97321 attttctgga tgagcactga ctgcactaga catgtagtag ctaaaagaaa caaaatcaac
    97381 agggtaggct gctaagattt cttcatcacc tacttctatt tttaagttga cacctttttc
    97441 ttcaaacatt cgttgcgtat acgctggata atatcctctt gcttgaacat cactaaagaa
    97501 caaagaagtt ctttgctgct caattgtttt ttgaatatca tccggatcac aagaatacgg
    97561 aacaagaagc tgatagctga tcatacaacc gacttgtgaa cctttgataa tttcatgaca
    97621 agcttttgta gctaaagcac ttgctataaa ctgatggtgt gcaccttggt atttcgcttg
    97681 ttccaaatgg ggatggtttt cttcaattac tcctacgcta acaaaagggt gatgttttac
    97741 acagttgatc tcattgaaag tcatccaata ttttacttta tttttatagc gtttgaatac
    97801 tgtttcagcg aaacgaacgt aaaactgaat aagctttcga ttaggccatc caccatattc
    97861 tgtaaccaaa tataacggca tttcataatg ggaaagagaa acgactggct caatcccata
    97921 ttttagacat tcgtcaaaca cttgatcata aaaaaataat cccgcttcat tgggtgtttc
    97981 ttcatcacca tgaggaaaaa ttcttgtcca tgcgatagac atccgatagc atttaaaacc
    98041 catttcagca aataaacgga tgtcttcttt aaagtgatga tagaaatcat ttccgtgtct
    98101 tttaggataa taatacttgc tgttgggatc ctgcgccgct tttacttgct gatgcgtcat
    98161 catatgccat tgatctgtcc atttttcttt aggtaaatcg ctgtcgaatg tatacacatc
    98221 agacacagat aatccttttt catctacatc ccatgcacct tcacattgat tggccgctgt
    98281 cgctccgccc cataaaaatc cttctggaaa ttttttcata tcgatccccc taaacttgct
    98341 agttttcttg tttgattatt tttcttatga ctagtaatga tatttttgaa tctactacta
    98401 tttgtaacaa taaccattgt ggtcggatca tatccctctt tgattaattc ttgcaaatct
    98461 acttccatca ataaatcgcc tttacgtact ttttttccat cagatactgc actttgaaag
    98521 tatttgccat ttaaattcac tgtatcaata ccgatatgga tcagtatctc tgctccttct
    98581 tcactgacta ccccaattgc atgtttcgtt ggaaaaactg ttgaaacttc cccgtttaca
    98641 ggactgtaga tttttccgtc cgttggaatg atcgcaaacc cttctcctaa agatccacta
    98701 gcaaatgttg gatcgtttac ttcagataaa tcaattattt cacctgctgc aggagtactt
    98761 aacactatat cctttgagga ttcttcttcg atttcatcct tgtagccgaa aagaaaagtt
    98821 agcatacagg aactgatcat cgctaagaaa taactagcaa ctagccaaac aaatccttca
    98881 ccaatataaa tcgggaaaga caaaatactt cttgtagcaa ctgccaaagc accggctcct
    98941 gcggcaccag ctactccacc aaatatagct ccacatatac aagcaatgta aaatggtcgt
    99001 ttatatttaa gagtcacacc ataaacagca ggttcagtga ttccaaataa tgctgtgata
    99061 aatgcagata aagataaact ttttactttc ttatttttcg ttttcaaaaa taccccaagt
    99121 actgctcctg cttgcgcagc tacggcagtc atacatgctg gacctagcgt atcacgaccg
    99181 tagaaagaaa gattgttggt cattactggt acaagtcccc aatgtaaccc aaaaatcact
    99241 aacacttgcc aaaatccgcc gataacagca ccagataaaa cagggctaaa gttataaaca
    99301 gctaaatatc cagctgcaat cagctgactt gcataatctg caattggacc tacaactaat
    99361 aaagtaattg ggaccatcac gatgatacaa atgaatggtg tcaataaatt tcgaatactt
    99421 tcgtgcaagc ttttgcgaat cttaggctca atataagaaa gaacccatac agctaaaatt
    99481 gctggaataa ccgtacttgt ataccttgct agaatcacgg gcatccctag aaatcgcaag
    99541 gttatgctat cactatatgc actgacaatc gtaggataaa cgagagctgc tgcaataacc
    99601 atagcgatga aacgatctgt attgaacttt tttgcagcag tatatgccaa aaatataggc
    99661 aagaaataga aaaatgcatc agctgctgca ttcaaaatga tataagttcc tgattcagta
    99721 gttaataaat tcaaactcgt acataaggcg agtactcctt tgatcaaacc acttcctgcc
    99781 aagacgccta aagtcggggc aaaaacagcg gcgatcatgt ctataaaaga gtttaaaatg
    99841 ccttttttct cagttcctct ctttccacta gatgcatcat cttctagttt catttgtttg
    99901 gataatacgt catacacttc atttactgca tttcctacta cgacttgaaa cattcctcca
    99961 gcttcgacta cagtgatcac accttctagt tgctctacct tctccttatc tgccttctta
   100021 ttatctttta gggtaaagcg taaccttgtt gcacaatggt aaacattgct gacattttct
   100081 tcgcctccga caaatcggac aatatctttc gctaattgtg aatagtccat ttttacttcc
   100141 cccttgttaa ttaatgccgt agcaatcctt cttacaagat aagtatagtt cttagaaaac
   100201 ggtttctcca ttcaattagt accccttata atagcgaacg aaactgtaca atagtgtgta
   100261 tggtttactt agccgagcaa atcactcact gcttgaaaga aaacaagtat atcgtttctc
   100321 ttatagtcaa aggagacaaa aaaaacacgc ttgatcttcc tttgctgatg tataagtggt
   100381 atctcaagct gatactctgt gatttttgtt tctacttggc ttttgataag acagcacaac
   100441 aaagtctcgg catttaacgg aagcataact aggtcagaac taggagagat tagttgctga
   100501 atggaatcta tctttttatg gatttttatt tgattttctt ctttgtagta atgatcaagc
   100561 agttttgttt tctttttttc ttcaactatg gtcagctttt cacgcaaaat ctgataaatc
   100621 tcccaagatt caaactcatc tagaacacta ggtagataaa tcaaatcatc ccactcaaaa
   100681 ataagctctt gctcagtagt caaaattgca tcatattccg ttattttgtt tgattgattt
   100741 ttgaaaagat acactggttc tatccggcat tctttgatcc acatgttcat actttgttga
   100801 atctgttttt ttagtgtatt caaaatacta attggctggt tactaacaat cagtatctcc
   100861 ccttgttttc gaaaaggatt caacgcttcg gcaagataca aaacaaaata agcgctttca
   100921 gctaaattgg gacgtaattg aaaatgtatt gggaagaaac gccgaattaa ataaatttct
   100981 aaaggataac gggagatgat cttttgtaca aaatgattca tggtattatg tccggcatcc
   101041 atcctcgttt tcatttgttt gatatgggtt agcaaaaatt ctgtttcatg gaacaataac
   101101 gaaacaggaa gatctaaaaa acgaataatc gtttgctcaa atgctcttag attcttctct
   101161 atttctttgt ttttttcatc attaatcatc aaataattta actctaatag tcgattttca
   101221 attgcttgtt tttcttgatt ggaaaaagaa tagggtagtt ctgatgataa cttttttgtg
   101281 agggtctcta ccatcggaat aaataatgta tacctgtttg ttttttctaa aagttcttct
   101341 cttcgaagaa tggtcaaaac gagataccga gaaaatcgta tgaagtcctc tcctgaaaca
   101401 atataattat atgatataag aacctctttt aatgtctttt ggacagtcgg catgatattg
   101461 gaaaaaattg tactgatttg ttgaggcaac cttgcgtgtt ggatcaccgt ttctgttcca
   101521 acaagagata agaaaattct ttttgaattt tcttcagcaa tgatttttag tccttttgtc
   101581 gacgaaactt ccagttctaa tttaggattg cgctcgatcc aacgcaaaat cgtttttatt
   101641 tgtaatgata cggctgtttt tgaaagaaag aaaatgtctg ctatcttttg catactgaca
   101701 tacccttttt gaaacaataa gtacgtcaca atagttgaag ctcggaaatc catgctggaa
   101761 tagattttat gtttcgtcac ctcttgtatg atttgctgtt tagtttcaga ttcaattgat
   101821 tctaaaaaat aacctgaacg tttatttgac tggataatga ttttcttctc gtagatagca
   101881 agcatatttt tgatttcagt ttggatcgtt ttcttgttgg tacctaaagc ttcagctaat
   101941 tcttcgcttg ttatccaatg attcttattt cttaatacta caagaatttg gagttgtcgt
   102001 ttatttaagt ccatttgctt acacctcctc tttgttataa acatacagga aaaacctctc
   102061 aaatgacaat agtcacttgc agaggtttaa aaatcatttg aagaaagatc tatattcaag
   102121 gtttttacca ttagactgga taatttgttt ataatattca aaactatctt ttttcaatct
   102181 cctaaagctt ccgctcccat cattttgtct atccacataa ataaagccat atcgcttcct
   102241 catttcccct gttcctgccg atacgatatc gattggtccc caccataagt aaccaatgat
   102301 ttggcaacca tcttttattg cctcagcaat ttcctgaaga tgtattttta gatactcaca
   102361 acgttttgga tcgtggattc gattcaattc atctggtgct tcatctagtc caatgccatt
   102421 ttcaactatg aataatggta attcataacg gtctgtcaat tcattcatga cataacgaaa
   102481 ccctagagga tcaattggcc aactccaagg ttcgggagat ttttccttca aaaagggatt
   102541 atcgaaacca tttgctccgc cagtatctac gcgcatttct gcttcttttt tatagacagc
   102601 gctacgataa taactaaatg caatataatc tgccgtgtta gcttgcaagc actgttcgtc
   102661 tccatctaac attactggtg cacagtcttc ttccttccat gtcctgtaaa tataagctgg
   102721 ataatgccct cggcacatga catcggagaa aaaccatgaa ttgcgtttaa attgcagtga
   102781 accaaataca ttctctggat cacagtcata tgcataagtc gctaatgcag aaaagctaca
   102841 cataataccc atcttagcat ttggatcgat tttctttaat aattgagtag cacgggcgtt
   102901 cgctagaaac atatgatgac aggcttgata aatcactttt tttgttagtg tagcaatggg
   102961 ttcaagttta tctctagcag ggaatgtaac caatccagca gctgcataag gcatacgaaa
   103021 agcattattg atctcattaa aggtcatcca atatttcact ttcccttggt agcgttcaaa
   103081 gacggtctgc gcataaaatt caaaaaagtc gattaatttt cgattttgcc accccccata
   103141 ctctcctacc aatgctaagg gggtttcata atgactaaga gtaattactg gctcgatatc
   103201 atatcgattt aatgtattga ttagtctgtc ataaaatacg agaccagcct catttggctt
   103261 ctgttcgtct cctcttggaa atattcgtgc ccatgagatg cttgttcgaa aagcttttaa
   103321 acctaactcc ttcagtaaac gaatatcctc ttcaaatcga tgataaaaat caatcgcttc
   103381 atgagaaaga taatggagtg attcgtctaa agcaatctcc caaatctttt tttcttcatt
   103441 ccactgaata ctaggatgct ggttcatgat cccgataagc gtatctgtca cattaggtaa
   103501 tttgccgtct tccaaccatg caccttcaca ttgattagca gctactgctc cgccccataa
   103561 aaaattttct ggaaatattt cctgatccat aactatctct cctcgctatc aacaatttct
   103621 tacttgaagt atagctcgat tcatcaaaac aagatattct aactatttcg gcagaagaga
   103681 agaaaaaaat tgaatgctac tcttcatttt taatatttat atataacaaa tacataagac
   103741 gatccccgaa agaaaacact cgttaaccat ctaagcaaac gatttatttt acttccaaca
   103801 caaatgcttc ataaggtcgt aaatcaactt tgccgtctgg taactgtttc agaggataat
   103861 ttgacacaat aacccttaaa gcttcacgtg gaacagtgaa cgtctgcttt tcactggaca
   103921 tattacaaat aacgagccat tgctggtctt catatatgcg agaataggca aagacatgct
   103981 caggcgtctt ttccactaat tcatagtctc cccagacgat gatgtcatgt tttctacgca
   104041 aatcgatcaa tttcttgtac atataaaaaa cagaatcttg atctgctaac gcttcttctg
   104101 cattgatttc tttataattt ggattgactc gaagccatgg aattccagta gtaaacccag
   104161 catgttcacg ggcgttccat tgcataggcg tcctagcatt gtctcttcct ttcgcattga
   104221 ttgattccaa gatttcctct tttgtaaatc cagaagaaat cctttcgtta tacatattga
   104281 ttgtctcaat atcttttgct tccgcgatat catcgattgg gcaattcgtc atgcctaact
   104341 cttctccttg atagatataa ggggtgcctt tcatcatatg gagcaagata gcaaacatct
   104401 tggcactttc ttttcgatac acatcgtcat ttccccaacg agaaacaatt cgcggaagat
   104461 cgtgattatt ccaaaataaa ctattccatc cttcatcacc taaagatgtt tgccatttgg
   104521 ataatacttt tttcagttca gagatactca agggtttcaa atcccatttg tctttgcctt
   104581 cttgctgatc taatcctatg tgttcgaatt ggaagaccat ggataattca tttcttttag
   104641 gattggaata aagtttagcg atttcggtag ttgctcccca tgtttcgcca acagtcatca
   104701 gatttttgtc accaaaagtt gcttgattca tttcctgaag atactcatgc agtttaggtc
   104761 cattacttgt gatttcttta tccggttctt tgccaatcat atcgatcacg tccatacgaa
   104821 agcccccaat acctttatcg atccagaaat tcatcatttg ataaatctct tggcgaacct
   104881 gtttattttt ccaattcaaa tcaggttgac gcttgctata caagtgcaga aaatattgcc
   104941 ccgttttttc atccaactgc caagcagaac cactgaatat tgagcgcaat ctattaggct
   105001 cttttccatt gaccggatca cgccaaatat aatagtcccg ataaggattt tctttatttt
   105061 tacgggcctc aatgaaccac ggatgttcat cagaagtatg attcaccacc agatccatga
   105121 tgatacggat atttcttttc tttgcttctt caatcagttc ttccatttct tccatcgtac
   105181 cgaattctgc catgattgct tgataattac tgatatcata cccattgtca tcattcggag
   105241 actcgtatac tggagaaagc caaattgcat caatacctaa atacgccaga taatccaacc
   105301 tggaaataat cccccgcaaa tctccaatac catcttgatt tgaatcttga aaacttcttg
   105361 gatagatttg ataaacaact gaatggtgcc accatttttg ttccatgatt ttcctcctat
   105421 aaatggatgt ttctattttt tgcttaatca tttattttag tctctctcac taaaaattgt
   105481 aacgctttca aatagctatt gcaccttgtt atcggtaaca gaataaaaca aaaaaacagc
   105541 tgaggaaatt ccctcaactg ttttttgaaa ggattgattt atgcagggaa gaatgctgat
   105601 ttcacaactt ctttataaac ggtgttcact cagctgtccc ttggtattag ctcaaaaaac
   105661 ggcaaaatat gaggaactat tttttcgttt ttttctctaa tactcaggcc agaacactga
   105721 tttcacaacc tctttataaa cggtgttcac tcagctgtcc ctcggtatta gctcaaaaaa
   105781 cggcaaaata taagaaacta ttttttcgtt tttttctcta atactcaggc cagaacgctg
   105841 atttcacaac ctctttattt cacattcaat atccacatga atcccacaaa tacaaagaac
   105901 agaccataca tgattggaga tacttctttt cctcttttag ctgctaacat ggtaattgga
   105961 tagaagataa aacctaatgc aatcccatct gaaatactat aagtcaaagg catgccgatc
   106021 aagatcaaga atgaagggat cgcaatctct aagtttcccc attcgatttg tcgcaaagat
   106081 tgtgccatca atactcctac cataatcaat gctggtgcag ttacttgtga agtaacaact
   106141 gatagtaatg gagaaaagaa caagcctaac aagaagaaaa tccctgtagt aatagctgtg
   106201 atacctgaac gtcctccaac ggcgatacca gctgatgatt ctacaaatgc accaacagga
   106261 gaagtaccaa ataaggaacc cgcaagcata gctgttgaat cagaagctaa agctttccct
   106321 acacgaggca tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc aactaaagtt
   106381 cctgctgtat caaagaaagt cacaagcaag aatgtcagca ctacgaccca catttgaaga
   106441 gaattgatgt cccctacatg ttttaatgca actaaaaatg ttggtttcaa acttggtgct
   106501 gcggcaacaa tttcagaagg cattggaatc aaacctgtga ccagacctaa aatcgttgta
   106561 gctgccatcc cgatgaagat gccacctggt acacgccgaa caagcaagat agcagttacg
   106621 actaatccaa aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa tcctactaat
   106681 gtagattcat tcgaaacgat gatcccacct tcgctcagac caaggaaagc gataaataga
   106741 ccgataccgc cagaaatcgc atatttcaaa tctgctggga tcgcatcaat gatcatttcc
   106801 cgcagcttga agattgtgat caaaataaaa atcaaagaag caacgaatac gccggctaat
   106861 gctgtttccc atggaatccc cattcctaaa caaacagaat aagcaaaaaa agcattgatc
   106921 ccaagagcag gtgccgttcc aattggataa cgggctaata cccccattaa aacacagccg
   106981 atcgcactgg ctaatgcagt cgcagtaaac actgctcctt catccattcc agaagcacct
   107041 aatacagttg gatttacaaa taagatataa gccattgaga taaatgttgt aaatcctgcc
   107101 agtacctcac gttttaccgt ggtgttcaat ttttctattt cgaagtagga aagtattttt
   107161 tccttcatct tccatccccc taaatccgaa cattataaat aaagtccgtt aaaatttcga
   107221 tttattaata aaacaacgtg ttttatcata tactaaaaca aaacataagt aaacaaaaaa
   107281 ttcggatttt aagcaaaatc cgaacgttcc atctgattaa atccctttat ctcaaagttc
   107341 atgaacattc actttggatt acaagaattt ctttattgtg ttaataaagt ttcttcattc
   107401 tagtctcaaa aatcaatagt gtgatttgat tttaccattt cgtttctgtt cttctttgat
   107461 gaattctttt gtatcgacag gattcagatt atttagtccg ccaaaacctg tatcgaccat
   107521 ttttccccag ccttttgtct tcttttttcc tgatttgaga ttctttgctc gaagcttctc
   107581 actatgtttt gctcgccgat acttcaaata accttctaat cttcccatta gatccactgt
   107641 ttttcctcct ttttattcaa tcagacgact cagcttttcc tataacaaaa taattgcctt
   107701 cagggtcaga aaaattgaaa gtcaagatgc cattgttatc tgtgatttct cctggttctt
   107761 ccatgttctc gtagatagtt tcaaattcat ccgtaaaaaa tactaaagaa ggcggtggtc
   107821 ctaatacctc tggtgaaaac ttctgcacaa atacttttgg gaatacgcct aattctaatt
   107881 cttttgagat gctgagtaca atactcttaa attcttctgg caattcgatt gtttcagaaa
   107941 tttctgcacc aattttttct acaaagaatc ggcaaatcat ctctacgtca ttgacataaa
   108001 gcatcacacg tgttttttgt atcatcattt gacccttctt tctatttgat tttctttcct
   108061 gtctataaac tgattccatt atatcacgaa agcgcaaaca ccttgtgttt gatactattc
   108121 aagaaaaata aagcaatagt ttctaggatt gctttcctcc acaaactttt tttacaaaaa
   108181 aaaaaacggc aatgaatgcc gctcttctta tttcgattct tttataattc tgcttctgat
   108241 tcatctttgg ttagtgtgtg tctaacacta gttacgccaa atgtttgatc gattttttcc
   108301 ttcaacgctt cgattgcctg tgcgccacgt gccgcttgtt cagcattgat ggattcaatg
   108361 accagcaccg ctgcttgtgt ttcttccgtc agcattgttc tttgggcttc acgccagttg
   108421 agacagcgac agatcgctcc ttgttcatca tagtagatga tttcttccgg aagtgccgga
   108481 gcatcggaat ctgcacctaa aggcaggaat gattcgccac cttgggcttt tcctaaatgc
   108541 agatttcctg cgatcttttc gatatcttct ccgccgcaag gtacgccaaa agacaaggaa
   108601 atactattat agatatctac aagcggattg attgggaaaa attcccgttc ttgatttgca
   108661 cgtttcagta aagcttcaat agaagaacgt gcaccttttt tagtcttgaa tgtggtaaat
   108721 gcctgacgcc actgagcaat gacttcgttt tggctgaatg tttcttctgt aaggtaattt
   108781 cttgcttctt ttgctgcctc attcaataat tgatgatacg tttcttcact ttcttttgtt
   108841 acgtggttat cgataccatt tacggataag atttcgatgg ttgcttcagg aaatacttcc
   108901 cagaaattgg tatccactat tacttttttc atctgttcac acctcttgat tagaataatt
   108961 gcttgatgaa tagatagtct gtttggggat catctcccat ataaaaaaca tgttcgctgt
   109021 atttgataaa tcctttttta cgataaaaag cgagcgcaac atcgttgtgt tcccaaacac
   109081 cgagccaaat ggctgttttt tgcttttctt ttgctttttc aatagctgta tctagtaaaa
   109141 atgtaccgat accttgatgt ttaaattttt ttcgtacata gattcgctcg atttctaaaa
   109201 aatgatcagc catttcctca gattgactcg ttcctgtatt tagtttcagg taacccgcag
   109261 gctcttgatc gatactcact aggaaaaaat cggtttcttt atcttttagc tcttctgtaa
   109321 gtttttctag tgcataagct tgtgtcaagt aatcagctaa atgctccggt gaattatctt
   109381 ttccaaatgt atctgtaaat gtttcgatac taatttgttg tagtaagtct acatctgaag
   109441 gcgtcatttt tcgaatttcg tatttattca cggcttcttc ctttcaatag atccttttat
   109501 tgcctttttt gacaaaattc caatcttcgc taacattctt gcatacttta tttaaaagat
   109561 attccaattg ttttgcttct tcgtcagaaa gaccatttaa tgcgactgca ttggagtagt
   109621 tgttttctcg aatgatgaaa ggatagattt ctgctccttt tttagtcgga tagagtttct
   109681 tgattttttt attttcttta tcttccaaac gttcgatcat cccattggat tctaatttct
   109741 tgattgctcg ggcagtcgtt gtacgatcta ctttgatcat ttctgctagt ttttcttgga
   109801 tgattccagg attttcgcaa atacgtacta aataaagata ctgtcctctt gttaaatcga
   109861 cttctttgaa ttcgatattg ctaatagaat ctagcgctcg ggcaatcatt ccgatgtctc
   109921 gaaggatttc agccatttca tcccaccttt attttgtttg taagactagt ttacctcttt
   109981 ttgttgcaaa tgcaactatt ttcaataaaa aaatcaagtt cttttttaat aactagtaaa
   110041 gtatattctt ttcttctttt acgaaaaagc tcaatcacaa tagatctggt ttttgttttc
   110101 taaaataagt aactgtttct tcgttgttcg cttttctgat agctttggca actcttctat
   110161 actaaaaaat cccatgttgg aagtttcatt attctcaata aacttggcct cttcaccgtg
   110221 gatcgcacaa gcaaaaacaa gtttataata ctgaaaaagc tgtggaatat ctttttgttt
   110281 attcgtatca aaaactgctc gcaactgagt agtttctact gttaatcctg tttcttcata
   110341 gacttcttta cgcacatttt cttcaggaga caatcctatt tcagcaaatc caccaggaag
   110401 tgaccattct ttcgtccgtt tatcttcgat tagtaggacc ttgccatctt ttttgatata
   110461 ggcacgaaca tctacttttg gtgttggatg tccttctcca atcgttgtga gcgcttttat
   110521 ttcttcgaaa gtttcttttc ctatttcact gatcagttcc aatgaaatcg tccggatctc
   110581 ctcaaaacgc tctttatcaa atacatcttt tccataatat agaccagctt gagaaagtgc
   110641 gagtagtctt ttgtattgag ccaacttctt gttcattctc ttcccttctt ttttagcaaa
   110701 tgtatgacta tgtaatcaag tatagaagac tggtttaatt acggcaaata aatataatga
   110761 aatctgcaat aaataaaaga atgttaaaaa actcgagaac aaaagacatt cgacttctct
   110821 ggggctgtga ataaatggcg ttcaaaagct tgcatctgcg gtaataagcc ccaccaaacg
   110881 aaaaatatga cgagctattc ttgtctggct gttcttatta attgaggcag gacagctttt
   110941 tcacaacctc cccactaaac ggtgttctcc taccaactcc tcggtcacaa gtcacaatat
   111001 tcatcaaaca tgagaagctg tttttttgaa attgctcctt gtgactcgga gctgaacgac
   111061 aggctcacga cctctcggta aacagcgttc aaaagccaat ttttcgatat taagccattt
   111121 ctttgttact tattgtcatt gatttctttt tctaatttat ccaatagtgg agtgtaatcc
   111181 acttgagtat agtctgttac atcaaattca tatagtgttc ctaactggaa tttgtcatat
   111241 tttcggtcgg cgatgatctt tttgaatgct tctttcgtaa tatgattggt ccctttcgtt
   111301 cgatcttcca atacgtcacc atagtgataa tgatccatga tatagctcaa atgacgatct
   111361 gattcacgat cacgtttgta ccgacgttga cataacactt caaagtctgc tgttaggcga
   111421 atcgtgatca cctgatatcc gtatctatct gctaattctt ggagtcgtgc tttttgctta
   111481 tcactaaaag gatactcgga cacaatcact ttctttcctg cttccatata caaatcaagt
   111541 gctttgtaat agaaaggata aactttcgtc ctttcttgtt ccgcccgttc ttccaaattg
   111601 ttaaacccga tggattctgc atagtattcc ttgatttcat cgagcgtgat ggcatacata
   111661 tctggaaatc gttcccgtag tttattcata agatatgttt ttcctgtacc aggactacca
   111721 gccagaagaa tgaaatactt tttcatctca tttatttcct aagttaattg gactttccaa
   111781 gttcataaac agtccaatct tatgcttgac aatttctttt actgcttcat ttgctggaat
   111841 cacattatgg cgaagagact tgttgatttc tgtttcacta aaggtttctt tagctacttt
   111901 cgtccagttg actaaaagct ccgttccaac attgaatttg cggatcccgt tattgatcaa
   111961 ttcaggataa tcttcttctt tcacacctgt tcctccatgg ataactaggg ggacatcaac
   112021 tactgcatcg atttctttca gcaagggaat gtttacttct gtttttgatt tgaattgtcc
   112081 atgattcgta ccgatagcaa tagctaatgc atccacgcct gtagcttcta caaatttcac
   112141 tgcgtcttcc ggtcttgtat ataccttgtc attttcagcg acatggattc cttcttctgt
   112201 accgccgatt gttccaagtt ctccttctac ggaaacaccg tgcgcatggg catattctac
   112261 tactgctttt gtcttcagga tattttcttt gaaaggcaga tgggacccat catacatgac
   112321 agaagtatat ccgctgtcga ttgcttcttt gatatcatca aagtctcggg cgtggtctaa
   112381 gtgcaagact gcatcaacca tttcttcttc tgcaatagct gtcattacat tgactaacac
   112441 atgcatgccg atatatttag cggtatcgac acttgtttga ataatgatcg gcgaacccat
   112501 ttcttttgct gcgcctagca tagcaggaag catttctagg ttatgcgtat taaaagctcc
   112561 aactgtaaaa ttcaactctt ctgctgtttt tgttacttct ttcaatgttg tatacatctt
   112621 ttattgccac cttatctttt tatttgtttc ctttgatcgt tacttgtttt gcaatttgtc
   112681 gcatctcatc cattttgccc atgtagtaat cgatcccttg atcatcacca gcacgagcag
   112741 catctgcacg cttctggtta tagaggctca tcagtttttt atacccatct ccgatcgtct
   112801 gtttattttc caaactcttt tctaaatatt ggatggcttg atcgttcaca tacaattcag
   112861 aaaatttaag tccaacttgt tcataaagtt tcgctaattt atgcttatct tgggtagtgg
   112921 aaatctcttg tttcaattgt gtaatctctg attgaatctt ttcggtcgct tctggcgata
   112981 gggctttttc ttcttttgcc agaacttttt tcggttcttt ttcttttttc ttaaaaaatc
   113041 caaacatcat tttccctact ttcaattaaa atgctttcaa aactgggctt aagatccagg
   113101 catataagag tgctgcggct actgtatcta catcggttgc tgtagcattg atgaatccca
   113161 tcgaattaaa gatcgttact agcaaagcgg gcaataatgt aataaagaaa ccatgagcaa
   113221 taccgccaat gatcgctcct cggcgtccgc caactgcgtt gccaaatatc cctgctgtcc
   113281 ctccggcaaa gaaattggtt agcataccag gtaaaatcat cgctaagccg aacatgggca
   113341 aagtaaacat ggcaataatc gtaccaatcg ttgtcgtaat gaaacctaaa atgacagcat
   113401 ttgggctata aggaaagaaa actggacaat ctaaggcagg gatcgcatca ggaactaatt
   113461 tcatagctat ccctcgaaat gccggcacga tttcacctaa cagtagacgt acaccagcta
   113521 gcaatacata cacacccact acgaattgga tggcttgtaa aaaggcaaac atgatatagt
   113581 tttgatctcc tgataattca gaagcgaatg gttcgcctgc aaataaaaca gtgatgatat
   113641 atagcgggac catcacgacc atgacagaca aataagtatc ttgcagaaat tcgaaatgtg
   113701 tcggcaactt gatatcttcg atcgactttt tattttcgcc tttctcacca aaaagcttag
   113761 caacaccggc ttcaaataag tacccgatcg tacaaaaatg acctaaagca atatcatttg
   113821 atccagtgat tttacgaatg atcggttgcg ctactgccgg catagcaatg gcgaaacaag
   113881 ctccgataaa accaccgacc aagatcaata cgatccctcg cagaccggca aaatagccaa
   113941 agactgtcgt catcgttgcc atccataaaa ttgcttgacc agttaaaaaa atgtacttcc
   114001 attttgtgaa acgagccaaa ataatattga atacaaaaat ggctaagaac gtcagtgcaa
   114061 tatctctacc taaacccaaa tcattcattg cttgaccatt gatagattca atagaaggga
   114121 tgatcccttg catatgaaat ccttcagtaa agattttccc gaaataagtt aaacttccaa
   114181 cgatgatgct cgaaccggca ctcaacactt gaaatcctag gagtgtcttt aacgtgccag
   114241 agatgacttg tcctgctgat ttcttctgca gtagtagacc caacatagca atcaatgcaa
   114301 tggtgatcga tgcctgagtc aaaatatttt cgatgataaa attgataacg cccatgtttt
   114361 taccctcttt tctttttgta tttagataac tcccttatct tgcagaactg gggtgatttt
   114421 ttcttcaatt tcattttttg aaacaatatt tttcaaatag atcatcgccg ttttaggatc
   114481 aactgcgaac ttttcgaact gcgaacgaaa attttctgct gtgatgatca catctggttt
   114541 catggaaact gcggaagaaa tatcacaatg gtccagtttg gcttctactc ctaatttatt
   114601 caaaacatcc tcagcagaca tctgtgctgc aaaactactt cctaaacctg caccgcatac
   114661 gaataaaatg ttcaatttgt tcatctttgt tctcccccgt atcatttatt atttctcaac
   114721 tactgaaaaa agcagttgtt tgaaatcttc gatatttgtg cattcaatca atcgatccaa
   114781 cttctcttcg ttgttgatca attcaattag ttctttcatg atgttcagat gcgagtagga
   114841 gtctactgct gccaaacaaa aaataatctt tactggatcc atttcaggat taccgaaatc
   114901 gattggttgc tcaatcgtag ctacgctgac tcctaattta ttgaccccat cttctggtcg
   114961 ggcatgggct agtgccatgt gtttgccaat cacgatatac ggtccgtaat cattcactgc
   115021 acgaatcatc gcttcgacat agcgctcttc gacaatgttt tctttaacca atggtctagc
   115081 agctagttca atcgattcct cccaagtagt ggctttttct ttgatcatga tattgctgtc
   115141 cgtcaaaata tcttttaaca taggctggat ctcccttttg ttgatttcta aattgtttgt
   115201 ttcgaataat ttttctaact tgccatagat gattcgatct actcggccac cactttcttt
   115261 catgacatct attatttgag tgaataactc cgtcgattca tgactgttag gctctaaccg
   115321 ctgtagttct tcatttcttt tcaaaaagtc ttctataacc aagatattcg tctcagttaa
   115381 aatcggatcg acaactaaaa gcggcttatt tcgataattc aattgaaagg tgctgaaaac
   115441 cagattgaca tccaatttat ctactaattc gatttctttt gaacttaaaa ctgcagtaat
   115501 ctcgatctga ggaaactttt ctttcagatt ctctgctaac agatttccag tagccatccc
   115561 atggttacag acaacgactg atttataaac ataattcaag tctcgcttga ttacactggc
   115621 gatcgtcgaa aagtggatga caagaaaagc tatctcgtcc tccagtatct tgccaccaat
   115681 tacttcttcg atcgtaggaa caaaatgagt gactgcgcgg tagattggga aataatttgt
   115741 tcggatgttt tctttcaatg gattcatgac ttggatatga ttcttgattc ttacaatcag
   115801 tgcagccatg tgcttataga gattttcgaa taaagcttct tcctttagat ggaaaggtac
   115861 gtgtgtctcg ttctctacaa attggatcaa ttggatcgtt agtaattgag cttgtaccca
   115921 ctcgattgaa ttggagatat ctctagcatt gaacgtctca atagtgaaac gaagataatt
   115981 tctttctact tgcgggacat tgtccaattg aaattcctcg atcatgtggt caataaatgc
   116041 agaaaagcta ctgtctttta tttctgtatt tttccaatta atggcagaga taaattcatg
   116101 tttcctgatt cgctggatcc agatcaatgt aaaaagcagg atttgttttt tataaatatc
   116161 atcttcttgt ttcaacaact tattggaata gatctcctgt atttttttga tttccaccaa
   116221 aggaaaataa tggagaaaga ttttttgagc cggtgttaat tgtgcatctt gattcataaa
   116281 gaagtcggtc attcccagat tctgattgat aaaatcatag atcatcgtac ggatagatcg
   116341 ttctgaacct ttataaacca gtccttgctt tccataactg acgatctcta tcccataact
   116401 gaccagtgtt gctcgtaacc gtctcatatc ttcatctaat gtacttttag agatttgaaa
   116461 ttcttcttct tttttgttta gatggatcgg gattcggctg taagcaacgg ctaaaactag
   116521 atcaaaaata cgttcgcgcc gattcaaaaa gccgtctttc acatctttta tttcagtttc
   116581 taatcgagct tgcttttcct tttccagctt caacaaaaaa actttgttgc gaattgtacg
   116641 gatctccgga agctttttac tgctcaacca gatattcgct tcaagaactt cctgacggat
   116701 cgtccgctga ctgacattga actcacttgc tagatgtttt gcttttactg gatattgggc
   116761 tgtcatgatt ctgtgaagta tttcaattgt tcttgttttc atagaaatga tctccttctg
   116821 atttcaagta tatggttccg cttacataat aaaaagtaga tatttctgcc acttgaagtg
   116881 gaaagaaagt aagcaaagca aacaaaaaat aagccatatg ctttttcatc tcctcaacga
   116941 aaaagacata cgcctattat tacaaaaatt ttgcccatct atacaaaaaa gaggttgtga
   117001 aaaagctgtc ctgcatcaag taataagaac aactaaacta aaatagtgct tcctattttt
   117061 cgtttggttg ttcttattat cgcagatgca agcttttaaa caccatttag tggagaggtt
   117121 gtgacagaga tgttcagcct tgagcgataa gaagcgattt tgaaaaatcg cttcccatat
   117181 tttacaaaat cgtgccttat tgccgatagg ctatctctgg cacactgttt attcagaaaa
   117241 aggagctgcg acaaggcttt tgtcacaact ccgttcataa atatttcctg tcaatccagc
   117301 ataatcggct gaataaaatc ggcttgccat cttttttctt tctcagaaaa atcaaaatac
   117361 catatctgag aaaaatactg tcgttgtgtc ttacttcctg agatcaatcg cccatctttt
   117421 cgatcgactg aataatcaca gcatctgaaa tcaattcgga tggcaaaact attttcgtgg
   117481 atctgtcgat aatttcctac ttcctgaata gttatttttt tagtatgatt tcttacaccg
   117541 tctgctttgt ttttttgcag aactttctga tgttcggcaa acagcttgtc tgtataacag
   117601 cttctggctt tttcaggagt ttcttcttcc caagcggatt ggatcttcat gaatatcttt
   117661 tggatttcca gaaactgtgc tttcttttct ttagccgttc ctgacatgcg ggacaatatc
   117721 tccgctttca gccttttctt ttgtctttta ttcttgacga tagaaaaagc agaaacccct
   117781 cctactgaca aaacagctaa tgcaagaaat cccgtttgct cataactaga aggacctgta
   117841 cctggaccat aatacccata agaatctctg ccgtaagaac gactgccaaa ataactgccc
   117901 ccatatgaac gaccaccaaa tgaacctctg cccgaacttc gaccgctaga tgtacctcca
   117961 ccatgaccac ccgcgacagc gaatccttgg gtcggcagag caaacaaaca taggcccact
   118021 aacaaaatga ttgctaaata attgattttt ttcattgtct tcctcgttat cttttattac
   118081 aaaaaactat ttttatgata gccttgctaa aaggaagagt caataaccag atgcataaac
   118141 tgttttttta gcaattttgt cttatttagt cattgagtaa catcggacaa atgtatgtgt
   118201 cggcagtcca taattcctct atcttcagca tgaacaagct tgcagacaag agatctgcgc
   118261 ttcctccagg agagatttgg ttttcctgac aaaagtgcaa cagtgctgta aatggacgat
   118321 ttggtaaagc actttgacta gcagcatgag caagtttttg catttccact aacgttttct
   118381 gatcactgcg atgcaatacg catgtgtcat ctagcgaact catcaaaact aacaacatat
   118441 gcatttgttt ctgtacatat gaatcattat ctagctcctt agctgcttgg attgcaagcc
   118501 tgatatgcgg ataccctaat atcgcttctt catatgcacc atttacgcca tatttttgct
   118561 tcaccctatg accatgagtg gtttttttat gacgatattt tgtatcagga aaagcagcaa
   118621 gcttgctgac atcatcaagt attttcgata aagaaaaagg ctgttccata ctgatttttt
   118681 gtgcaatgac actgatcaat agccccattg cccaaatcgc tcctttatgg gtattgatgc
   118741 catttgtagc aatcagcatt gccttttccg cttttcgccc aatctcagca attctttctc
   118801 ttaactcctg atcaatagca tgatgccatg ctatttgagc catttcttca aaaaaaggag
   118861 ttagcgtctt agcagactta agaaataaag catagtccat atcggaatgt gcacccgcat
   118921 ccagtgcatc cactaatcca ggttttggac ttaatgttac ttctgcgatc aatgcttcct
   118981 ctaccaacgc agcgagttgc tgagtcactg ctttttttcg ttcgtcattt atcatgacac
   119041 tcactccttc ttcctactat tacttgctga aacctttttt gatagctacc caaatgatat
   119101 gaagtaacca gcttaaagca aatgcacaac ttgccgtcat tagataagct acaagaatca
   119161 tgactcctga aggtaactgc agccaactat ctttaaacaa taataacagt acttggaaaa
   119221 caaatacatt ggctaaatac gtacgataag cataaacaga caaccatttg aacaatggca
   119281 attttggcga atcgtttaca atcattttgg aagcgcctat gaagacagca aagatgatga
   119341 ctaaactgta taaactttgg atcgtattca agtaaggtgc attcgcaaag ctcaagtctc
   119401 ctggatgtgc gagcaatgaa cggacagaaa agaacgcgat aactaaggca acaggtaaga
   119461 aaagataacg tactctgtcc aaatattgat agactttttc gtgataaaca aatgcggcta
   119521 ccccataaat catgaaacta gcaacaaacc gatctaataa gtaccagctg tcatgataag
   119581 gcccagtcaa tacttgtgtt tgatagaaca gataccatgc gacatataaa atcgttgaac
   119641 caatcaatac tggccataca tattttttat tagcaaatac tttgtaaccg agccaaacaa
   119701 aaaacggcat gaacaattgg atctgcagca tcataacggt ataccataaa tgaggcgctc
   119761 catcacctgt gacatatttc agtaaaaagc ttgcagtatc cgtatacgga gtttgttgtt
   119821 ggactgctgg aaatagccaa agataagccg tcgtccatag aagatacgga acgactagtt
   119881 caaaaaactt ttctttcaaa aattcccaat aagacaaatg ctgacttgtt tttaccatat
   119941 tataaacaat tgcaaaaata aacattggtg ctgtgtattt aacgatgacg taaaaggtac
   120001 caagaaaagc tgtatttgtt gcgctgtggc tgtttgccaa agcaaagctc aatatcgttt
   120061 gcatcataac agccgttaca gctgatactt tctggaagtc acttacgttg attttgattt
   120121 ttttgttttc aaacatctct ttctctctct ttttctttat taccaaagtt ctttggttaa
   120181 tttaggtcct tcgactgttt tcaatagaat ttttttctct ggctctctca aatactcttt
   120241 tagtgaaaaa ccgccttgcc gagtcactac ttgggtatcg aattgctcaa aaaaagcagg
   120301 atgcgattga attacctgaa ttggcaattc aatcggtgaa tttgcgtaca gcagcaaatc
   120361 aaaatcactg gtttctttta ctgccggaat cccagtcgat aattcaaatc ctaagctgcc
   120421 gccgattccc catttacatc ctgataatat gtttctagca gcatgaaatt gctgataaac
   120481 aggataatcc atgattttct cttgtctata cgattcttgt tcgatgatgt cccacggatg
   120541 aatgacttgt ttgacggcat gttttgagat ttctgttgac catctctgat tgcgggcagg
   120601 tccacgtaaa ccaatcggta gtatttctgc gtgatccgtc gacgcacgac gaactgttcc
   120661 gaacatcttt tcagttagaa cgacccattc aggcaaggtg aacccttcta tttcatgttt
   120721 atcaaaaagg acaatatcat gtgcttgaat cattttttat gcatcccatt cttcgtccat
   120781 tttggcacgg actttatttg tttcgacacg tccgaatgtt ttggctgttt cgtttgtgta
   120841 tcgatttgtc agcatcgtgt catttgtatt gcggacatct tgaatggctt cattgattgc
   120901 ttcgatgact ttgctgacat tttgttctga agcagcttgg tcggtgatat cttctaggaa
   120961 atgatagatc gctcctagtt ttgtataatt attgatatcg taagcgatag ctggaacttt
   121021 ttcagcagct gcttcgattt cagcaaccga acgttttgtc acacgtgcag cactttcttt
   121081 agacatcgct tggacggtaa tttctttatt gtttaaagca atcaagcggc ttgattgtaa
   121141 tccatgtgct aaaaatgcac ctgaaatagc atttcctgca atcaatccaa taactggatg
   121201 tccagcttga cggagttttg cataagcagc agcactatta gccaaagcca catggatacc
   121261 gatcaattct tcgttatagc cgtatgcttg gcttggtaca tcgatcacaa cgatgattgg
   121321 ccgtttcact tttttgtctt tatcttcttc gtaaacgtga tccaaaattt gtcccatgcg
   121381 gaaaccttca actaaaccaa cttcaccgtt tcgtacacgg tacatcgggt ttttaggatc
   121441 aggtacgatt gatgtcacga tggcttcttc tatgaattta cttgatttac cataatacac
   121501 agtactaata gaagaagttg gatttttcat gccagttagt gccgcaaacc aatcgtaacc
   121561 aacacttgct gcagctggtt catttccttc tgctacttct ggtaagtcaa ttgattttgc
   121621 ttgatggtta gcaattgctt cattgtaatc gtcaatggcc attggttttg aaagatctaa
   121681 agaatcaagt aatgataaat aaaatgctga gctttcactg cgatgcgtgt cttttcgttc
   121741 gtccattgct gcgatgacag cagctttgat tgcttctaca ctatcagtca ctaattcatc
   121801 aatgattttt gattctaccc gttgacgaga tcctaatgta ttccaaatca agtctttgtc
   121861 actagaatca aattctcgaa cgcctgcttc ttgttcaata acttctggtc cattcaaacc
   121921 gactctcgct ttttttgttg cgattaaata actcattaat gctgaagtaa tcgacatacc
   121981 gccaaatgat cctacacgtc ctggaatcaa accgatgact ggtacatatt gttttagagc
   122041 aatgattaca tttccgattt ctgaaataga taacagtccg tagtttgctt cttgcaaacg
   122101 aacaccacct gtatctagta caatgactgg aaagatctca tttccttttt tattttcttc
   122161 taacacatgt tcaagtgcag caacgatttt tgcaccggaa acttcaccga taccgccacc
   122221 ttggaatttt ccttccatag cgattactac tgcttgtttg ccattgatcg tgccacggct
   122281 gacaacgatc ccgtcatcac tttcagggac gattccttgt gctggtaaac ttggcgcgat
   122341 gatttgatcg aatggatcaa tcaattcacg tccattgtca agtagagcgt tcactcgttc
   122401 tctggcgttt aattctacaa aactattttt catctgaaag ttcctccatt gcttgcgtca
   122461 atcgtaggta cacaactcct ggtgtagcac cgaagtcatt gatcacgata tctgctgaaa
   122521 ttggataacg atcaaaaaat ctcgttaata cattgttcca tacttcgccg aagccatcgc
   122581 ttcctgtaca aatttttaca gtagattctg ttttttctgt tggaaacatc aagatttcca
   122641 aatctcctga accgaccact cctacatgga ttttcttatt gatcggctgt ttcgttggat
   122701 atttataagc taattgttcc atggatctct cccctttttg taaaacagag catacacgtt
   122761 ctgcttattc gtctttcttc ttccatctgg gtaaatggcg acaaaaggtt tttctcgcca
   122821 attttatgac caagttctga atttcattgg tggttcatac aggccatctg accattcgat
   122881 caaatcatcg atggtttgtg cagctaataa tgaacgtttc gcttcactac gtttgatgcc
   122941 tagatcttct ggtaatttca cgatgccggc tgcacgcaag ctatctagtg attctttcgt
   123001 gctagtcatt ccaagaggtg taacaccgcc aattgctgca attgctgctt gtcgttcttc
   123061 ttttgttttt gctttgtaca aataagcaat tccttcttct gtcacgatat gtgtagtatc
   123121 ttcgctataa atcatcactg gtgcattggc taagcctgct tgtttttgaa cagctagtgc
   123181 atctaattct tcgacaaaga ctggtttttt atttgaacca tatgtttcaa ccatttgaac
   123241 aactaatttt tggcctttcc ctaatggatc atcattgtcg cgaagtgatt gccatgctgg
   123301 tgtagaatga cgacgaccgc caggattatg tcccatgtta ggcgcaccgc caaaaccaga
   123361 taaacgtcct tttgttacgg tagaagagtt tccttcgtaa tctaattgca aggttgaacc
   123421 gataaacata tcgatggcat attgtcctgc agcttgtgat aatgttcggt tactgcgcat
   123481 cgtgccgtct ttaccgacaa aaaagacatc tgggcgagca gcaatatatt tttccatacc
   123541 aacttcgcca ccaaaactat ggatgctttc aacccaacct gattcgatgg ctggaatcat
   123601 cgttggatgt gggttcaata cccagttctt cgcgattttc ccttttaacc ctagtgattc
   123661 accataagtt ggcagtaaca attcgatagc tgctgtattg aatccaatac cgtgattcaa
   123721 agattgtacg ccgtgttctg cgtagattcc tttgattgcc atcattgcca ttagaatttg
   123781 caattctgtg atgttttgag gatctcgtgt gaataaagct tctaattgat aaggtttatc
   123841 tgctttcacg acaacgtcta cccagtcacc agggatatct acacgtggta aagaatcgac
   123901 aacttcgttg acttgaacga tcacgatacc atctttgaaa gctgcagctt cgatgattgt
   123961 tggtgtttct tccgtattga aacctgtgta caagttgccg tctttatctg ctttatctgc
   124021 agctactaaa acaacatttg gaatcaaatc gatgaataag cgtccataaa gttccaaata
   124081 agtatggata tcacctaatt tcattgtgcc gtcttcaatc atttgggcga cacgcaagct
   124141 ttgtgcacca gcataagaga aatcgatctt gcttgcgatt cctttttcaa aaatattcaa
   124201 atgttctggt cgtgagatac tagacatgat caaatgcaga tcattcactt tttctgcatc
   124261 tactttttca agactttctg aaagaaaact tgcttgtttt tgattgtcgc cttctaatac
   124321 aactttgtcg ccgggagcaa tcagtttttc cattacttct acgatttttt cagtttctac
   124381 atacttgcca ttgagcaagt tgctgatttt ttccaatcgc tgcgcttttt tttcgcgttt
   124441 agaagcccag ttgcggtttg gtttttccat aattagtcat ctccattttt gatatagaaa
   124501 cgtggcgtca tcaataccat gttggtctag attgatcgta cggatccctt tatctacacc
   124561 aaattttgta tgaatcagct ttgtcaaagt attgccgggg ggaaattcaa tcgtcagatc
   124621 cattcctaat tcttttgcca agtccatcat ttggttccac tgcacaggga aagctaggtt
   124681 ttccaacaaa tctttacgaa tctcttctgc gtcctgagtg gctcttccat ccgtattttt
   124741 tatatacaaa catctagcat cagatagatg gacagactgc atatacggct tgaatttctt
   124801 gaccgtatca ttcatcaaga gacagtgaga tggcacaggg actttcagca gcttcgctgt
   124861 ttgcgcttgg tgccgtttgg cttctgccaa cgtaacggat aaaccttcga tactcccaga
   124921 taacgtatgt tgcatcggac aattttcatt tgacagataa actggatggt caggatcaaa
   124981 tgtttttgct acaatttcct cagcttcgct tctcgtcaca cctactacta cgcccattgc
   125041 ataaccgctt ggataagctt ctttcattaa gactgcgcgg tgatagacta gttccgctgc
   125101 attctcaaat ctcaaagatc gacaagcaac agctgcacaa aaagcaccaa tcgaatgtcc
   125161 tgctgcaata tcaggctgaa taccgttttt ttccatttca tctaaataaa atactgcttt
   125221 tgtcagtaat gctagctgga taaaaaccgt atcttgatag ttttcttctt tatcaactaa
   125281 ccgatacccc gtcacatttt ctaaacgttc catgtacatt tcagggatat ccgctaacat
   125341 ccctaacttc tgactgcctt gtccaggaaa taagaaacct gtactcatga gcctcgcctc
   125401 ctcagcactt actttttcca ctgctatctt agtcaatttc tgctataata aaaaatgcca
   125461 aaaaaagaaa aaaccataac aaaaaggtat ggatgggatg gataattatg aaattgcttc
   125521 atctgcagta ttttaaaaaa gtattcgaaa caaaaaatgt tacccaagca gccaaagaat
   125581 tatttatttc tcaacctgcc ttgagccgtg cgatcaaaca tttggaaaac gaattaggtg
   125641 tccctttgtt ttaccatagc ggcagaaaca ttgagacgac cgtttatgca gaagaatttt
   125701 atccatatgc agtcaagaca ttagacacat tagaagaagg aatcaaatta ttgtcttcgg
   125761 tcaatgagaa gatcgttact tccgtcatat tgtatttaga ggtagcctct gtttctattc
   125821 cgaacttggt ccgaattttt atggaaaagc atcctgatat ccaattaacg atcatgcaac
   125881 atcatttacc agatgatatt accgatcctg tactgtacat cacttcggag atgaagcctg
   125941 gcatgaccaa tattcctatc atgaaagaac ctgtttatgt cgccattcct aagaaacatc
   126001 ctcttgctaa aaaagaacat ctgcaattag aagatatcca acgcaccccc atcttgatgc
   126061 tgtcaaagag caacgccttc cgtcatacac tggatgcagc acttgaagcg aaaagtatcg
   126121 aattgacgat cagttcgacg acagatgatc ctgcgacatt acgttctatt gtcaagcaag
   126181 gattagctgt tagctttttc ccaaaagttt cttggtctta cgataaaaat gatcctttcg
   126241 tcttgcggga aatcaaagat ttgcccttga cacgaacaat ttatttatct tctagcttca
   126301 cagaagatca tcctttgatc aaaatgattg caaaaacgat gcgggaattt tatttaggaa
   126361 aataaaaaaa aaagagctgg gacaaaattc ttgtcgcagc tcttttttcg aacaaacggt
   126421 gttccagaag taatttctta gaaaataagc cgaaatttcc caaaaatttg agaagcaatt
   126481 ttcgtaaatt ccttcttatt tcttgaagct aaacatttct gtcacaacct cttctctatt
   126541 gtcctagaca ttcattgcgt tgatttaaaa cttccatcaa tcaaatctgc tagttcatct
   126601 agcttttcta ttttacgtag atcaatagag atccaatgtt ctttattcat gtgataggct
   126661 ggaaaatacc cctcttttcc tcgcaaaatt ccgccaagtt caggatcgac cttcaaatca
   126721 agaatattga tgatcctatg gtcacttgca tcatcataga ttttttcttc tggaacagac
   126781 atcaggaccc caaaccattt tttgcttttt tgatgtctga atacgcaata atcaggaaat
   126841 tttttaaaaa tatattctgg agacaaattg tatgtttcat tgatgtgatc gattagttct
   126901 tttttcgtta gcatctgctt ccatctcttt cttattgatt cgctttattc aaagatacca
   126961 cgtaagaata gaaaaatgaa tacaagtagt tgctaatcaa atacttcgat atttttcttt
   127021 tttatcaact cttaaatgtt caatccgcct gtgttggtct tgctaccatc tactattaaa
   127081 atgtttatta ttgtttgccc acctattttt tgattttagt ggtatcattt ttcaagttca
   127141 tgatgaaatt cttatcatca gtaaatgctc cagaaaaata aacaatgatt gaaaaatcag
   127201 ttgaaacttg accaccgttt attttctccg tattcttaga atagaaggga gtctgattga
   127261 tgtattcact aatgaaaaaa atcattaccg aaaaagatat tcgacgtcat gtttctttag
   127321 tagaacaatt gctgaatcat acaaagataa ccgtaaatga attagctgaa atcataggga
   127381 caaccgaacg aacgattttt tctgaccttc aatctattcg ctctcagctg cccgaaggct
   127441 gggacatcat cagcgatcaa gcaggaatta gtttgcaaaa ccagcaaaat ttattaacaa
   127501 atgacctttg ggagattttt ttcaaacaat cagtcagtgt cgaactgctg aaaaatctgc
   127561 tttttacaaa aaaagttgct gtccctgatt ttctagccga ccatgggctt tcttatggga
   127621 cattgaaaag acatgtgact aaaatcaatc agcgtttagc aagttacgac ttgcagatcg
   127681 atttgacgaa atatacagct tgtattcttg gaaaagagcg tgcgattcgt actttttatc
   127741 atcgattgct gattcctttc actcataaca actatttttt cgaggattat tcgatccatg
   127801 aatcccatta ttttcaattt ttaagaaacc tcagtcaaac tgaactagcg gttgaaacag
   127861 aagagatttt cggaacatgt tggttcttta tcaatacgat ccgtataaaa gcgaattgcc
   127921 gattagattc ttctatccat accaacagca cactctcctc cctgtatgac tctgcattga
   127981 aaaaattgta tttaacggaa ggtatttatc ttaaagacac tgagctttct tttgcttctt
   128041 tctgctttct ggaaagttgg aattacaaca ataactatgg acaagaaacc gctcgctgcc
   128101 tgcatcattc tccattttta gaggtcctcg aaacgtttgt agaggaacta gcttcagagc
   128161 tgtctcttga tcaactaaaa aaaacatctc tgactgataa cttggctctc ctcatattaa
   128221 aatatcatga atcgccgata ttgattgaag cattggaccg tcaataccat cgttttttag
   128281 aaacatatga tcagcaatat gcacacttgt atcaacagaa agagaattta ctgaacgatc
   128341 taaaaaaaga aatagaaatc aatgaaccaa attactttct ccaactcttg tctctactga
   128401 tccaacaaac gattttttct atcaaaccga atttgttcaa tgtctatttt attttccaag
   128461 gagaaccttc atggaaggcg ttcttgcagc aggaattaaa agattactta ggaaaacgtg
   128521 tctgcttctt acctatcgaa ctaaccgatc tttccactat ttcttttgaa aaaacagata
   128581 tcttgatttc caatttccct ctagattctc tagatatccc tattgtatac atttcttcta
   128641 ttccaacgaa aaatgagctc aatcgtttga ctgaactcac tttcaaaact tttttatagt
   128701 aacggctagc gactgtattc tctagctgtt ttttcttttt tgcgaaacag tttgttcatg
   128761 atcccttttt ctatttctac aactaagaat agaagaatca catttgctcc aatcaataac
   128821 tgttgtgtca tcgttaaact tactgtaccg atcagctgct ggccgactgg tagaaaaata
   128881 accatccctt gcaataaagc aaggatacct aatgaaagaa aaagcgcttt gttttgcaaa
   128941 agaccttggt tcaatgacgg cttggacaac tctctgcaat tgatcatgta aacagcttga
   129001 gctaagacga tattttgcag aagaatggtt tgctgtaagc tttgcccttc aaactgcata
   129061 gccaataaat aggcaggaac catgatcagt agggagacat agatgatccg aaacaaatga
   129121 taaccagata ggattccttg ctttggatct cttggtgggc gcttcatgat ttctggatct
   129181 ggcgactcga accctagttc ataagataaa gtgattgtcg ttaccatatt gacccataaa
   129241 atctgtactg gggacaaagg tagcgggcga ttcagcataa gtgcccaaat caagatcaat
   129301 ccttgagcta atgcagttgg caagaagaaa ttgatggttt tttgcaaatt atccaaaatc
   129361 cgccggccct cttttacggc ttttgcaatc gtatggaaat tatcatctcc taaaaccata
   129421 tcagctgcct gtttgcttac ttcactacct ttgattccca tagcaattcc tacatcggat
   129481 cttttcaaag caggtgcatc atttacgccg tctccggtca ttccgacgat ttctccattt
   129541 ttctgcaaag ctttaacaat tcgcagttta tgttctggtg tcgttcgagc aaagacatcg
   129601 actattggca cttgctgaat caactcttca tcagacataa gatccaaatc gatcccttct
   129661 aaaactttgg cagtatgttt taagccaatt tgttccgcaa tcgcttttgc tgtgtctttg
   129721 tgatcccctg taatcatttt cactgagatc cctgcttgtt ggcattcctt tacagctttg
   129781 actgcacttt cttttggcgg atcgatcaat ccggcgatcc ctgcaaaagt taattgggtt
   129841 aaagtctcat gcgtgagttc ctgtttcgaa tctacttttt tataagcaaa tcctaaaaca
   129901 cgctgacctt ttctcgcaaa ttctgcagct gtctgactcc atgcttcttg ttctgcagaa
   129961 gaaagagttg atttagcaaa taatacttct ggtgcacctt tgacgtaaat caccgctcct
   130021 ttttcctccg catgcatcgt tgccatatat ttgtattcag aactgaacgg aatctttgct
   130081 tctatttttt tcaaaggtag attggctgtt tctgtgtgtc gaatcagcgc aacttctgtc
   130141 ggattgccac ttaaattttc tgttttctgt tcttcttttg tttttaattc ctgacagttt
   130201 ttcataatgg acaacacgca ggcttcttct tttgcagcta catccacaac tgtcatttca
   130261 ttttttgtca atgttcctgt cttatctgaa cagatcactg tcatagaacc taatgtttct
   130321 accgaaggaa gtcctttgat gatcgccttt tcttttgcca tttcatgtac tccaacggat
   130381 agaatcatgg tcaaaacagc tggcaatcct ttgggtacca ttgacaccac caaagcaatc
   130441 gtagccgaaa gaaggatatg ccaatccata ccatatcgaa atgtcgtaaa aataactaag
   130501 aataggctca aacaaagaat gccttgaaaa atttgtttgt ttaatcggtt gattttttta
   130561 atcagaggcg tcgtttgttg ttcgaccgat tgcaatgctt gattgatttt accgatttct
   130621 gtcgtgtttc ctgtttctac aacgacaccg actgcagaac cagattgtac aagcgttcct
   130681 gaaaaagcca tatttttctg gtcgccgatc gattcttctt ctgtcaaaac ggcatgactt
   130741 ttttcagatg cttctgattc tcccgtcaaa atcgattctt ccactaataa atcatgtgct
   130801 tcgattaatc ggatatctgc tggaacgaca tctccggcac gtaagacaat gacatctcct
   130861 tttaccagtt ctttcgcagg gattttttct tgcatcccgt ttgatagtac tatcgcttct
   130921 tgtcccatca gctgcttcaa accattcaat gattcttccg cttttctttc ttgccagtaa
   130981 ctaacgagtc cattgacgat cactacgaat aaaataatac ttccttcaat atattcacta
   131041 gaagcaaatt tcaaaaaaga ggcaatgatc aacacgatca tcagcagatc agtaaaatgc
   131101 tttgctattt ttttccatcg ttttgtctgc tgccttgctt ttatttcatt atatccttga
   131161 tcagtcaggc gtttctgtcg ttcttcttgc gacaagcctt ctgttgatgt ttgatagttt
   131221 ttcaatacat cttctaccgt caatctgtac cagttcatcc tatcccctcc tgtttaattg
   131281 atactgttat tatacgcagg gaagtttttt caggcgtatc gattatttca ggggggtctg
   131341 aaataattcg ttttgcaaaa tgtatctggg cctaactgat atttctattg cttattcttt
   131401 tatatacaaa atttaagaat acaaaccaga taaccaataa aaaaggaata aagcgtgtcg
   131461 tgctttcgaa taacaagctg aaagcaattg aagtaaaaaa caccaacaaa gcaagatctt
   131521 tatatctttg tgtagtgttc cgtttctttt ctttaataaa ttgaacatgt gcacacaaga
   131581 tcgaagacca cacaaataga aaacaactcg ttgtcatgcc tgccaaaagc tgaaaaaaca
   131641 atcgactatc agctacaaaa aaatgtagac aaagaccggc aaaaaataac attcctgtaa
   131701 ttaaaatacc gtgtaaaggt acgcctttct tagaaagcgc acaaaaccaa tggggcaaac
   131761 ttttttcttt tgctgtttga tataaaatcc tgctagtact gtagactgca ctatttgctg
   131821 aggacaaaga agcaaccaaa atcattagat tgacaattgc tgctccgcct tggcaatgga
   131881 tcacttgcag catagtcact aaaggacttt cttttaaatc taaacaatgc catggaacaa
   131941 caaataaaat cgacaaaatc gttcctatat agaaaaagag aatacgccaa ggcagacagt
   132001 tgattgcttt tttcaaattc ttttgcggat cacttgtctc gccaacggtt aagccgatca
   132061 tttcaattcc agcaaaactg aataaaacca taggaaatga gactaccatt ccagcaaagc
   132121 caaacggtag taattcatcg atctgtccaa aattttcttt gattccgata gactgggtat
   132181 gaaaatctga gacactcaat cctataccca ataagataaa aaggaaaatc cccaagatct
   132241 tgatgattga gaaaaaagat tccaattcac caaaccactt gactgcaaca aggttgactg
   132301 ccattaagat cattagtgcg ctacctccgc ttatcaaagc tggtacatgt acaaaccaga
   132361 aatggatata caatcctatt gctgttaatt ccgcaagtcc agctccaagc cagcaaagcc
   132421 aataagtcca attgacaaaa aatgcccact ttttgcctaa ataatagtca acgaaatcac
   132481 tgatagagtt aaatgtacta tttgctaaaa ataaatcacc catggcccgc atcaaaccat
   132541 aaatagccaa accgcatatc aaataaacga gaataacagc tggccctgct gatgcaattg
   132601 cctttgctga acctaaaaat aatcctgttc caattgcgcc acctaatgcg attaactgga
   132661 tatttcgatt agttagagtc cgtttcattg ccggtttttc catcaagttc acctttttct
   132721 ttttcaagca gcatggcgac ttcctgttcc caagaaggtt tctttatttt ttcaatacag
   132781 agtggtacta caaacaaaat gacactgatg gctgtgactg caattagatt gatccatgtt
   132841 ccagctttca tcgtcagtcc gacagaaaat ccgattgccg caaataaaac aataacgaca
   132901 gtccaaactc caagattgct ctttttctct ttttttccaa ttctaaatgg tcgattcaac
   132961 tttgcatgtg tataacgcaa tctcaaaact gcgatcccca tgatgacata gacgatacag
   133021 tagataacag tagttgaatt tacgagcatc aaaaatgctt gattgacgcc aggaatcagc
   133081 aaataaaaaa gtgcaaagat tgagataatg acagactgag tcaaaataac ggatttggat
   133141 acgtcaaatt gattcgtctt ccaaaaattg aattttggtg gatacgcacc ttttctagca
   133201 ctttctgtca ctgtctttgc tggtcctgag gcccaagcag ataattggac tgctacacca
   133261 atgaagacaa gtaaactaaa cagattcaca ataagatgtg gcaaaccaag aatttggcaa
   133321 taaatcgaaa tagattgcgc gatattattc aattccattt gtccttttgg tacgacattt
   133381 gctacaacta aagcattcaa aatattgacg aaaaatataa aaattaacgc ggcaaatact
   133441 ccgcgaatat acgttttaac tggattttcc aatcgagtga tatacacaga ggacatttcg
   133501 ataccagtaa aaataaacat gatcggcgca aaataaacaa atgaggatgc agtcgtagta
   133561 tctggtatta atttgtccca tgaaaaactg cctaatgttc cggttggtac gatcccaact
   133621 ttgatccacg cagcaaaccc taataagagc atcatcgtca aaggcacgta caatcctaac
   133681 caaacaccga cttttccgcc gatttttgcc atatcaaatt tcaaattcaa aaaagtgatg
   133741 atccaataaa cgactaaaat gacaactaat gtaaatttgc tgttcaaacc aagcggcaca
   133801 ttatcgataa tattaccaaa caacggagcg agtgctgaag cgaccatcac catcccagga
   133861 aacatttgga cccataacag ccatgagaca acgaaccccc atttttttcc tagagcattc
   133921 gatacccaaa gttctggacc acctttttgg ggaaacattc cagcaaattc accagagatc
   133981 aatgagattg gaaaagcgta caataatact gcaaccagca tgtaaaaaat catcgtccag
   134041 ctcgttgctg ctacatctgg aatatttcgt acacttgcta caagggcgca ggtcattccg
   134101 ataaaggtca tggtcgtgat ttgctttgat gaaccagtat ctttcatctt tattccttct
   134161 ttcaatagcg acatatgttt ttgttttctt ttatttttgt tcaagaacag aaatgacaag
   134221 gtttcatcga ttcagtacaa aacccagtat caccgaaaca tccattgaac gatcttagtg
   134281 aactgggttt gatacatgaa acgaacgatc caacttatag aacgttttat aaaaaagaat
   134341 atcttggcga ggaatatatg aaaaacaggg ggaaatacaa ttgttttgat gaagcaaata
   134401 attgtctgtt ctccatatgt ctcttgttat tctttcacac aaaaatcagt tctattggat
   134461 gatttcgttt agtttctttt ccattgcttt ggcgattttt tttgtataaa aattgaaaat
   134521 tcgatcatca ttcaaataag gagtcatgat agccgcacgt agcagagtga ttttctgttc
   134581 ttttttccat tcttctttcg ttagacccat tctttcaaca aaacgaatcg gagaatcacc
   134641 gtaatcttct tgtgtaaatg tagtatgaga agtaataaac cgttcaccat aaacatcgcc
   134701 atccatataa gaagaccgat cgaacatttt ttcatttaat tcattgatag cgttcaaatc
   134761 tgtacagcct tgttctttga atacccaatt gaccatatta aaatcaggat gattcaatgg
   134821 atagacttcg attgtttttc ctttaacagt aaaagtcaga tgatcgagaa attctctgaa
   134881 tctttgagct gcctcgattg aagccccaat caattgacca tatccggtca cattcaatgg
   134941 caatacacga tgtgctgtcc agactgcggc ggcggttgct ccagcttttg atccttccaa
   135001 aatataagct cctaacatat ctggagcctt tactgatttt tcaaacacat atggcgcgaa
   135061 ataagaaatg atatttctca tatttttatg tttgattacg attcctccag cagcataagg
   135121 gacatagccc atcttatgag gatctatcgt gacagaatcc gcttctgaaa tagctctgaa
   135181 cccttcatat acctctttgt caatcgttac gcagtggtgg aaaacatgat gttcttcaaa
   135241 gaattcagct aaggaagcat acggcacaaa ttctcctgct tcgtttagaa acagtgaacg
   135301 cgcatagccg ccatatgcgg catcaacatg taaatagaaa tagatccctt catcttttaa
   135361 cctttctctt aattggacga ttttatctac actgtctact tgaccttctt ctgtcgttcc
   135421 tacgacagca accacaccta gaattgggat tttttgtcca gctaattcac gaattgtttt
   135481 ttccaaagca ttaatatcca ttcgataatc ctcttgaact gggatagcaa ccatctgatc
   135541 taagccgact ccgcaaatat ctaacgcctt catccaagaa tagtgttttg tttgagggac
   135601 cagccatttc cctaatcttt ggatattttt tccgctacgg gaagaagctg ctttgacctc
   135661 atccatttct tcgtccgtaa agctttcggt catttccaaa atttcttcaa cagataaatt
   135721 caaaagttcc cactcagaca tttttttcac tttttcaggc agcacttcct tgacagctaa
   135781 aggaatcgat ttgatgcaac gggcgtacca taatccttca agattagcaa tagaaccatc
   135841 tgctgcaatg tgtccccatc cgtctgtcat attgaataat gaagcaaaat cctgtcctac
   135901 ttctgcttcc atttgcgaag ttgccataga agattccaat gcaacattat ttgggttcca
   135961 cagcatcgca tagttgtaag ctaataaagc aggcatcaat gtttcagcat tcatttgtcc
   136021 ccagtaacgg cctgctgaat gccatgggat cgatcctgcg cgtaatcgct gactgacttc
   136081 atccaagacc tctatcatat ggtcgcgggt ggctagataa tctggagtga gcttatctcc
   136141 ttcgctaatt gcaggcatat ctgaaggaat ataattttct ctccatccaa gatgttcatc
   136201 taccatcttg ttcaacaaca ttttataaac tggtccattt tcagctttgt cgcctataaa
   136261 gacggccttg atatccatat ctttcatttt tcttccctcc atattttgtt ggtcaatgtc
   136321 ttgcagatac atgtgtttat ctgtaacacc tcttcactat agcgccaaaa atgagaaatt
   136381 tttttttata acatcaaatc cacgtagatt tttacaaaca aaaataaata aagtcggatt
   136441 tttctgataa ttcaacgcaa aaaagcgaac aatttttcaa taaatcagtc ttctaaaata
   136501 gataacctaa aaaaagcgaa caaacaaa
//
LOCUS       DAMYXM010000002       100000 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.002, whole genome shotgun sequence.
ACCESSION   DAMYXM010000002 DAMYXM010000000
VERSION     DAMYXM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 100000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 100000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.002"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            469..2106
                     /locus_tag="PNS21_000117"
     CDS             469..2106
                     /locus_tag="PNS21_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5157866.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2251..2991)
                     /gene="truA"
                     /locus_tag="PNS21_000118"
     CDS             complement(2251..2991)
                     /gene="truA"
                     /locus_tag="PNS21_000118"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDI5157867.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3182..3817)
                     /locus_tag="PNS21_000119"
     CDS             complement(3182..3817)
                     /locus_tag="PNS21_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDI5157868.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3964..5205)
                     /gene="sstT"
                     /locus_tag="PNS21_000120"
     CDS             complement(3964..5205)
                     /gene="sstT"
                     /locus_tag="PNS21_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDI5157869.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5403..6401
                     /locus_tag="PNS21_000121"
     CDS             5403..6401
                     /locus_tag="PNS21_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5157870.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            6414..8432
                     /locus_tag="PNS21_000122"
     CDS             6414..8432
                     /locus_tag="PNS21_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDI5157871.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8492..9208)
                     /locus_tag="PNS21_000123"
     CDS             complement(8492..9208)
                     /locus_tag="PNS21_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDI5157872.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(9435..9977)
                     /locus_tag="PNS21_000124"
     CDS             complement(9435..9977)
                     /locus_tag="PNS21_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI5157873.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9982..10209)
                     /locus_tag="PNS21_000125"
     CDS             complement(9982..10209)
                     /locus_tag="PNS21_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDI5157874.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(10288..12168)
                     /locus_tag="PNS21_000126"
     CDS             complement(10288..12168)
                     /locus_tag="PNS21_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDI5157875.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(12357..12971)
                     /locus_tag="PNS21_000127"
     CDS             complement(12357..12971)
                     /locus_tag="PNS21_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5157876.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            13113..13673
                     /locus_tag="PNS21_000128"
     CDS             13113..13673
                     /locus_tag="PNS21_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI5157877.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13978..14292)
                     /locus_tag="PNS21_000129"
                     /pseudo
     CDS             complement(13978..14292)
                     /locus_tag="PNS21_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(14546..16181)
                     /locus_tag="PNS21_000130"
                     /pseudo
     CDS             complement(14546..16181)
                     /locus_tag="PNS21_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16736..17299)
                     /locus_tag="PNS21_000131"
     CDS             complement(16736..17299)
                     /locus_tag="PNS21_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDI5157878.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(17314..18357)
                     /locus_tag="PNS21_000132"
     CDS             complement(17314..18357)
                     /locus_tag="PNS21_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5157879.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(18490..18894)
                     /locus_tag="PNS21_000133"
     CDS             complement(18490..18894)
                     /locus_tag="PNS21_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDI5157880.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(19127..19762)
                     /locus_tag="PNS21_000134"
     CDS             complement(19127..19762)
                     /locus_tag="PNS21_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDI5157881.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(20028..21080)
                     /locus_tag="PNS21_000135"
     CDS             complement(20028..21080)
                     /locus_tag="PNS21_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDI5157882.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(21093..22031)
                     /locus_tag="PNS21_000136"
     CDS             complement(21093..22031)
                     /locus_tag="PNS21_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDI5157883.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(22482..23132)
                     /locus_tag="PNS21_000137"
     CDS             complement(22482..23132)
                     /locus_tag="PNS21_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5157884.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(23232..23741)
                     /locus_tag="PNS21_000138"
     CDS             complement(23232..23741)
                     /locus_tag="PNS21_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDI5157885.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(23826..24305)
                     /gene="rlmH"
                     /locus_tag="PNS21_000139"
     CDS             complement(23826..24305)
                     /gene="rlmH"
                     /locus_tag="PNS21_000139"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDI5157886.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            24733..26022
                     /locus_tag="PNS21_000140"
     CDS             24733..26022
                     /locus_tag="PNS21_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDI5157887.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(26061..26666)
                     /locus_tag="PNS21_000141"
     CDS             complement(26061..26666)
                     /locus_tag="PNS21_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDI5157888.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(26871..27335)
                     /locus_tag="PNS21_000142"
     CDS             complement(26871..27335)
                     /locus_tag="PNS21_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDI5157889.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(27357..27677)
                     /locus_tag="PNS21_000143"
     CDS             complement(27357..27677)
                     /locus_tag="PNS21_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDI5157890.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(27821..28900)
                     /gene="pepA"
                     /locus_tag="PNS21_000144"
     CDS             complement(27821..28900)
                     /gene="pepA"
                     /locus_tag="PNS21_000144"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HDI5157891.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            29100..29450
                     /locus_tag="PNS21_000145"
     CDS             29100..29450
                     /locus_tag="PNS21_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDI5157892.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            29673..30620
                     /locus_tag="PNS21_000146"
     CDS             29673..30620
                     /locus_tag="PNS21_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDI5157893.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(30677..32095)
                     /gene="pepV"
                     /locus_tag="PNS21_000147"
     CDS             complement(30677..32095)
                     /gene="pepV"
                     /locus_tag="PNS21_000147"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDI5157894.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            32244..33089
                     /locus_tag="PNS21_000148"
     CDS             32244..33089
                     /locus_tag="PNS21_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDI5157895.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            33093..33533
                     /locus_tag="PNS21_000149"
     CDS             33093..33533
                     /locus_tag="PNS21_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDI5157896.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            33583..33777
                     /locus_tag="PNS21_000150"
     CDS             33583..33777
                     /locus_tag="PNS21_000150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5157897.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            33913..34599
                     /locus_tag="PNS21_000151"
     CDS             33913..34599
                     /locus_tag="PNS21_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI5157898.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIRRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(35007..35696)
                     /locus_tag="PNS21_000152"
     CDS             complement(35007..35696)
                     /locus_tag="PNS21_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5157899.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(35671..37800)
                     /locus_tag="PNS21_000153"
     CDS             complement(35671..37800)
                     /locus_tag="PNS21_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDI5157900.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(37848..39185)
                     /locus_tag="PNS21_000154"
     CDS             complement(37848..39185)
                     /locus_tag="PNS21_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDI5157901.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(39437..39796)
                     /gene="rplT"
                     /locus_tag="PNS21_000155"
     CDS             complement(39437..39796)
                     /gene="rplT"
                     /locus_tag="PNS21_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDI5157902.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(39849..40049)
                     /gene="rpmI"
                     /locus_tag="PNS21_000156"
     CDS             complement(39849..40049)
                     /gene="rpmI"
                     /locus_tag="PNS21_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDI5157903.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(40121..40645)
                     /gene="infC"
                     /locus_tag="PNS21_000157"
     CDS             complement(40121..40645)
                     /gene="infC"
                     /locus_tag="PNS21_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDI5157904.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(40689..40814)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            40965..41597
                     /locus_tag="PNS21_000158"
     CDS             40965..41597
                     /locus_tag="PNS21_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDI5157905.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(41599..42504)
                     /locus_tag="PNS21_000159"
     CDS             complement(41599..42504)
                     /locus_tag="PNS21_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDI5157906.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(42589..43248)
                     /locus_tag="PNS21_000160"
     CDS             complement(42589..43248)
                     /locus_tag="PNS21_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDI5157907.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            43564..44508
                     /gene="mvk"
                     /locus_tag="PNS21_000161"
     CDS             43564..44508
                     /gene="mvk"
                     /locus_tag="PNS21_000161"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDI5157908.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            44501..45478
                     /gene="mvaD"
                     /locus_tag="PNS21_000162"
     CDS             44501..45478
                     /gene="mvaD"
                     /locus_tag="PNS21_000162"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDI5157909.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            45491..46576
                     /locus_tag="PNS21_000163"
     CDS             45491..46576
                     /locus_tag="PNS21_000163"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDI5157910.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            46573..47637
                     /locus_tag="PNS21_000164"
     CDS             46573..47637
                     /locus_tag="PNS21_000164"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDI5157911.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(47886..48689)
                     /locus_tag="PNS21_000165"
     CDS             complement(47886..48689)
                     /locus_tag="PNS21_000165"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDI5157912.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(48709..49551)
                     /gene="kduI"
                     /locus_tag="PNS21_000166"
     CDS             complement(48709..49551)
                     /gene="kduI"
                     /locus_tag="PNS21_000166"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDI5157913.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(49567..50574)
                     /locus_tag="PNS21_000167"
                     /pseudo
     CDS             complement(49567..50574)
                     /locus_tag="PNS21_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            complement(50581..51225)
                     /locus_tag="PNS21_000168"
     CDS             complement(50581..51225)
                     /locus_tag="PNS21_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDI5157914.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(51325..52083)
                     /locus_tag="PNS21_000169"
     CDS             complement(51325..52083)
                     /locus_tag="PNS21_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDI5157915.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(52210..53361)
                     /gene="fucO"
                     /locus_tag="PNS21_000170"
     CDS             complement(52210..53361)
                     /gene="fucO"
                     /locus_tag="PNS21_000170"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDI5157916.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(53443..53754)
                     /gene="rhaM"
                     /locus_tag="PNS21_000171"
     CDS             complement(53443..53754)
                     /gene="rhaM"
                     /locus_tag="PNS21_000171"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDI5157917.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(53756..54601)
                     /gene="rhaD"
                     /locus_tag="PNS21_000172"
     CDS             complement(53756..54601)
                     /gene="rhaD"
                     /locus_tag="PNS21_000172"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDI5157918.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(54615..55871)
                     /gene="rhaA"
                     /locus_tag="PNS21_000173"
     CDS             complement(54615..55871)
                     /gene="rhaA"
                     /locus_tag="PNS21_000173"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDI5157919.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLEEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(55868..57328)
                     /gene="rhaB"
                     /locus_tag="PNS21_000174"
     CDS             complement(55868..57328)
                     /gene="rhaB"
                     /locus_tag="PNS21_000174"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDI5157920.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            57481..58350
                     /locus_tag="PNS21_000175"
     CDS             57481..58350
                     /locus_tag="PNS21_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HDI5157921.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(58476..59375)
                     /locus_tag="PNS21_000176"
     CDS             complement(58476..59375)
                     /locus_tag="PNS21_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5157922.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(59540..60364)
                     /locus_tag="PNS21_000177"
     CDS             complement(59540..60364)
                     /locus_tag="PNS21_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDI5157923.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(60375..61670)
                     /locus_tag="PNS21_000178"
     CDS             complement(60375..61670)
                     /locus_tag="PNS21_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDI5157924.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(61686..62165)
                     /locus_tag="PNS21_000179"
     CDS             complement(61686..62165)
                     /locus_tag="PNS21_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDI5157925.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(62171..63199)
                     /locus_tag="PNS21_000180"
     CDS             complement(62171..63199)
                     /locus_tag="PNS21_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDI5157926.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(63216..64322)
                     /locus_tag="PNS21_000181"
     CDS             complement(63216..64322)
                     /locus_tag="PNS21_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDI5157927.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(64551..67148)
                     /gene="adhE"
                     /locus_tag="PNS21_000182"
                     /gene_synonym="adhC"
     CDS             complement(64551..67148)
                     /gene="adhE"
                     /locus_tag="PNS21_000182"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDI5157928.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(67453..67833)
                     /gene="yajC"
                     /locus_tag="PNS21_000183"
     CDS             complement(67453..67833)
                     /gene="yajC"
                     /locus_tag="PNS21_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDI5157929.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(67930..69075)
                     /gene="tgt"
                     /locus_tag="PNS21_000184"
     CDS             complement(67930..69075)
                     /gene="tgt"
                     /locus_tag="PNS21_000184"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDI5157930.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            69293..69991
                     /locus_tag="PNS21_000185"
     CDS             69293..69991
                     /locus_tag="PNS21_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDI5157931.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(70159..72798)
                     /locus_tag="PNS21_000186"
     CDS             complement(70159..72798)
                     /locus_tag="PNS21_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDI5157932.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(73417..74448)
                     /gene="queA"
                     /locus_tag="PNS21_000187"
     CDS             complement(73417..74448)
                     /gene="queA"
                     /locus_tag="PNS21_000187"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDI5157933.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            74641..75816
                     /locus_tag="PNS21_000188"
     CDS             74641..75816
                     /locus_tag="PNS21_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HDI5157934.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            75820..76461
                     /locus_tag="PNS21_000189"
     CDS             75820..76461
                     /locus_tag="PNS21_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5157935.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            76458..77375
                     /locus_tag="PNS21_000190"
     CDS             76458..77375
                     /locus_tag="PNS21_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI5157936.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            77372..78043
                     /locus_tag="PNS21_000191"
     CDS             77372..78043
                     /locus_tag="PNS21_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5157937.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(78584..79072)
                     /locus_tag="PNS21_000192"
     CDS             complement(78584..79072)
                     /locus_tag="PNS21_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HDI5157938.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(79273..79539)
                     /locus_tag="PNS21_000193"
     CDS             complement(79273..79539)
                     /locus_tag="PNS21_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDI5157939.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(79539..79802)
                     /locus_tag="PNS21_000194"
     CDS             complement(79539..79802)
                     /locus_tag="PNS21_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDI5157940.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            80278..80463
                     /locus_tag="PNS21_000195"
     CDS             80278..80463
                     /locus_tag="PNS21_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI5157941.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(80605..82428)
                     /locus_tag="PNS21_000196"
     CDS             complement(80605..82428)
                     /locus_tag="PNS21_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDI5157942.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(83026..85731)
                     /locus_tag="PNS21_000197"
     CDS             complement(83026..85731)
                     /locus_tag="PNS21_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDI5157943.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(86037..86984)
                     /locus_tag="PNS21_000198"
     CDS             complement(86037..86984)
                     /locus_tag="PNS21_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDI5157944.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            87174..87443
                     /gene="rpsN"
                     /locus_tag="PNS21_000199"
     CDS             87174..87443
                     /gene="rpsN"
                     /locus_tag="PNS21_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDI5157945.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(87619..87981)
                     /locus_tag="PNS21_000200"
     CDS             complement(87619..87981)
                     /locus_tag="PNS21_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDI5157946.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(87992..89113)
                     /locus_tag="PNS21_000201"
     CDS             complement(87992..89113)
                     /locus_tag="PNS21_000201"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDI5157947.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(89129..89479)
                     /locus_tag="PNS21_000202"
     CDS             complement(89129..89479)
                     /locus_tag="PNS21_000202"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDI5157948.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(89609..91120)
                     /locus_tag="PNS21_000203"
     CDS             complement(89609..91120)
                     /locus_tag="PNS21_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDI5157949.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(91442..92794)
                     /locus_tag="PNS21_000204"
     CDS             complement(91442..92794)
                     /locus_tag="PNS21_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDI5157950.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(92947..94023)
                     /locus_tag="PNS21_000205"
     CDS             complement(92947..94023)
                     /locus_tag="PNS21_000205"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDI5157951.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            94219..95964
                     /locus_tag="PNS21_000206"
     CDS             94219..95964
                     /locus_tag="PNS21_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDI5157952.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(96244..96681)
                     /locus_tag="PNS21_000207"
     CDS             complement(96244..96681)
                     /locus_tag="PNS21_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDI5157953.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(96665..98038)
                     /locus_tag="PNS21_000208"
     CDS             complement(96665..98038)
                     /locus_tag="PNS21_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5157954.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(98360..98848)
                     /locus_tag="PNS21_000209"
     CDS             complement(98360..98848)
                     /locus_tag="PNS21_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5157955.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(98870..99472)
                     /locus_tag="PNS21_000210"
     CDS             complement(98870..99472)
                     /locus_tag="PNS21_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDI5157956.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            99571..>100000
                     /locus_tag="PNS21_000211"
     CDS             99571..>100000
                     /locus_tag="PNS21_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDI5157957.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT        29411 a        20930 c        17669 g        31990 t
ORIGIN      
        1 ctaatcagaa tcgtacaaat atttaataac caaatatata gaacataact taatattaag
       61 ttatttgaaa ttccataaaa aacgactgca ggaattattg taagaaaagg ataaaaatag
      121 ttcagtccat ctccataatt gttaaatgtt gtatagttga ttggcccttc aaaaatagaa
      181 gataaccctt tgattcgtga aagatgaaaa gataaatcac ctgttgtata aatttcttga
      241 gcatttctca taaaatttac tgaatataaa gtactaagta agataagaac tactaaaaga
      301 aatgcttttt tacttatttt catagatttg ttgttctcct tttttaattc tattttcagt
      361 atactagaat tgtctttttt taataagttt tgcgttaaat ttttcatagc actagtatga
      421 tatactagaa aagaatcaat ttttattact tttaaatagg agaaaaaaat gaaaaaaaac
      481 aaaaaccaaa taatagatat catttttatg ttatttctaa gttttgctta cgtcatccct
      541 ctattcatgt ctaagggaat ctatcatagt gtcaatcagg atacttactt tcatttatct
      601 cgaatcatag gactagataa tgtatggagt tctccagtaa acttcaataa cttcgatcat
      661 catggaacaa tgatgaatat tttttatcca tggcttactc tttatccagc ttttttattt
      721 tataaaatga tgggaaatct tgttttaagt tataatatct actatttttt tatcactttt
      781 ttaacaatgg tcgtttctta tttttctatg aaacaaataa aaaacaatcg ttatatttcg
      841 ctgttatttt cgattattta tacattttcc gcctatcgtg ccattgacat attccatcgt
      901 gcttcattgg gagaagctgt cgctttaaca tttttgcctt tgatactaat ggggtgttac
      961 gaaatttata ttagagatta ccaaaaatgg tactggttgt ctattggcat gacgctagta
     1021 gtgtatactc atttactttc tgtcgctatg gtttctgtat ttattggagg aacgttgttt
     1081 ctttcttttt atttttggga ccaaaaaatc gctagactct taagtctttt aaaagctacc
     1141 gtcctcacct tttttttaag tgcgggattc ttaattcctt ttattcaaca atctagagca
     1201 caagaattga aagtcccact gggaaaagaa ttaagtggta tggctcctag tgatatgctg
     1261 acacatattt taaataataa ctataacaac tatactattg gattattctt atttctcgga
     1321 ttgattggtg cttttatttt tataaaaaaa ttaactgctg atgatttatt tattttcttt
     1381 cttggtgtat ttgttctttt ttgttcaacc aatttatttc cttggcaact ttttaaccat
     1441 acaccagtta agagtctaca atttgtctgg cggttaaatg gtttttcttc tttatttata
     1501 gcctatacga tgagtatagt gatctattat attttttcta ctaaaaataa ttcatggaaa
     1561 ataatggtct ttacgtttct tgcactaata ttgcatctct caggagtaag caattctatc
     1621 catgctaaca aagattcatt aacacttata gcacctgaag acgctgttgc tatagctgaa
     1681 aattacaatc atactgatta tgcaaacaaa gagtcaatct atcatcctga tatgattaat
     1741 aatgatcaat atttactagg taaccaggaa ataaatccaa caactcattt tatgagtaac
     1801 aaacttacaa ttgaattaga taattcgaat aataaaaata ctgtcttgac gacacctatt
     1861 tatcgttata aagggcaagt ggcttctatt aatggcaaac tagtacaaac aaaattatct
     1921 aagtttggca caactgaatt aactattcct ccaggtataa ataaagtcgt tattacttat
     1981 cagtacacaa aactagcaat tgcttcaaga tatttatcta tcgttacttt gattttattt
     2041 ttactttatc gatttacttt ttctaaacaa aaacaaccga gaagagaagt acaacattct
     2101 cactagattt ttatttaaga aagacttttt agctgtacac ataaacaggc taaaaagtct
     2161 ttttttatat ttctttaatt tgcttcatac attatttaac atcgatattt atactgtgaa
     2221 acatgcgttt catccattat caaagtattt ttatcgataa taaacttcat ccaaaaacag
     2281 accttgtgca gggacggttt ctcctgcatg ttgccgcact ttgctttcaa atatttcatc
     2341 gattgtttct atcggcatgg cacctgtgcc gatttctagt aatgtcccca tgataatccg
     2401 caccatttta tataagaatc cgtctccgac aaaagtaaaa tgaagcattt ctccctttcg
     2461 ttcgatagat aattcatcaa tcgtacgcac tgtggatttt tttgattttt tcaatgcaga
     2521 aaaaccaatg aaatcatgtg tacccactaa tttttcgcaa gctgcattca gcttgtccat
     2581 atccaactct tgagggaaat aaaagctgtg gtttcgctca aatgctgaag gaatcacgtt
     2641 gttccaaaca taatagctgt attttttgcc caccgcatta tatcttgaat ggaagcgctc
     2701 aggcatttct tcgattcttt tgatgacaat atcccgcgga aggtaacgat tcataaagtc
     2761 gatcatttct gccagtccaa tttctgtgtt tgttttaaaa tttgctactt gtcctcgcgc
     2821 atgggtgcca gcatctgttc ttcctgatcc aataatttct atctcttcat ttgtcatctg
     2881 atgcaatact ttttcgattt ttccttgaat ggttttatcg tcatctccaa gtctttgcca
     2941 tcctgaatat ctttttccgt catattcgat tgtcaacttg atgtttctca tattttagtg
     3001 gctcctctgt cttccatttg gtcttctctc taccttttta agattgtagc acaaaatata
     3061 gctccttgct tctctaagac agaagctata caagaaaact gtggaaatat atatttcaga
     3121 aaaacaaaaa acagccaccg gatgaagtat ccgctgactg ttcaatttga cggctcaagt
     3181 tttactgttt gagatgcaag atactcaacc aggcaatggg cacataatta aagaaaaaac
     3241 tgattctttg gcaaaaacct attttattca gtaatggcca agaatttata gaaggaattc
     3301 ttgccagtcc ataaatcgcc gcaaaaagca aactgatcac agacagtcca aggtataagt
     3361 ggcttacgtc catttgccca ttttttcgat agatcaagaa aagaagaact ggaaacaata
     3421 aaaaaccact gtaacctaaa cctgatccga tattatggac ccatgtcgag acattccacg
     3481 aagattcatg ggtattgata ctgaacaaac ctgtaaagat acaatcacct ataccataga
     3541 acgcgatggc tgctgtaact agggtagcta acacacgaga tgtactaaca aatgcagcat
     3601 acaatgcagg caatgacagg atgaaaaaga tccctgaaac cacagaccaa ataagaaacg
     3661 cttttcttac aggactggaa acctctccaa atacactgat cacgctcttc atttgatcta
     3721 attcaggata atacaatcct aatacgtacg gagtcaaaaa atcactcagc acgcctaata
     3781 ataaaaagaa aaaaccaaat ttacgtaaaa aatccatgat acgcctccaa taacaattac
     3841 ctatgctttt aattgttatt ataaacgact ttatcttgat ttaacagaaa cctctattta
     3901 cacgaacatc tatataactc attaaaaact cgaatcattt taaaaatact aaatatctaa
     3961 cgttcaacgt tcacttaatc catctaaaca atctttcaat ttgatttttt ccccacttct
     4021 tttacgcgcg cctagctctc ctattgcagt aaacaacaga tcacttgacg aatttaaggc
     4081 agtttccatt gaatcctgga taacaccgat aataaatcca ataccgacga tttgcatcgc
     4141 gacatcatta gagataccaa acaaactaca tgctaatgga atcaataaca gtgaaccgcc
     4201 cgcaacacct gaagctccac aagcagctaa agcagacaaa atacataaaa ggactttcaa
     4261 aacaaaaggt gcttcaatcc ctaaagaatg tacagcagtc aacgtcatca aggaaatggt
     4321 aatcgcggca ccgcccatat tgatcgtagc acctaacggg atcgatatgg aataagactc
     4381 ttttgtcaat ttcaatcttt cagccaacat catgtttatc gggatatttg cggcagaact
     4441 tcgggtaaag aaagcgggaa gtgcgctttc tttcaaacaa aagaaaatca atggataggg
     4501 attttcttta ataaacaaaa acgtgaggaa tgggtacaca actaatgcga caaacagcat
     4561 cgtaccgatc aaaacaagta aaagctgcag atactctgcc aatccagcga ttccagtcgt
     4621 agcaattgat tgataaacca atcctaaaat ccctatcgga gcaaatgcaa taatcacttg
     4681 tacgacttgt gacaacgcta tagatatctg atccaccaac tcttttgtcg attctgaacg
     4741 tgctcgaaaa gctagtccca acaatacagc ccagaataag acgccaaggt aattcgcatc
     4801 aataagagat tgaacaggat tttgtgtgac attcgtcaac atagtagata aaacactttc
     4861 caaactttgc ggtgcttttt cagatactgt ttcttgtaaa gtaataggaa cagtaaaaat
     4921 tgaacttaca ataactgcta caagtgcaga aagcaatgtt cccactaaat ataagatcaa
     4981 aataggcttc acgaatgtct tattcccaat tttatgcttg gcaattgacg ccatcgtcaa
     5041 gaaaaaaacc agaataggtg cgattgcctt caagcaactg acaaacaacg ttcccaatat
     5101 tgaaataaat gtccatgtgg ggaaaaagat accaaacaat attccgacta aaataccaac
     5161 aacaatacgt tgaactaacg acatatccaa aaacttctct aacaaatgac caccctttca
     5221 caattttaac aatcattgta tcattttatg caaagaaact aaaggtataa ttttctaaaa
     5281 attctaacaa tacaaacaac catttcttgc taattttgta ctttttctgt ctacacgttg
     5341 ttttttgaga tattctaagt taggatgtta ttaatgggca acttatcagg aaacgttttc
     5401 ttatgttttt gtccaatttt tttgtgaggt gtttattctt gaaaaaaata aagaaggaga
     5461 gttatggctt cctgacaatc tttgtcgctt gtataatgat ggttttacag cgtatattga
     5521 tttcagcagt cactattcct acatatgcaa caaaccctgg tcgattctac atctatacat
     5581 ttttacaaac ttccgttgtt attggtgcaa atttgatccc actttacatg ggttaccaat
     5641 atcaaaaaat cgcccgttta aaagttttac attatttaag tcgttttgcc tttatattcc
     5701 taatagccac actagtttgc aatatctttt tttacgtaca agctggcagc ttgaatattc
     5761 gtgattattg gcttatccta acacctatca gtcagaatta ctttacttat gctgtttctt
     5821 gtactttgct tttttgtagt attccttata cagttggatt tttaaataaa ttatcgaaaa
     5881 caacaattaa atatctacta ttatttttaa caattggatt agttggctgc tctactctat
     5941 ttaataaaga cccgtttgca ttacagaatg gacagagtat tttatggatt ttttatttat
     6001 ttttaatggg ttatggtttg aaagaatgga actggaaaga aaaggtccgc tataagcttc
     6061 ctcaagtatt tatcagcttg attgttttat tcggtttgat tattttgatg acacaaattt
     6121 cactcatcat cagaggaaat acagatacag cgctgaggtt ttctaaacca tattctctct
     6181 tcgcaatgta ttatactttc tcttcgtttt tatttttaga aatgctatct gataaattag
     6241 gtataaaagg aagaggaaat tttcttgctg tatttttgat tgtcactcaa gttatatgta
     6301 atttcccttt agtttcttat cgcgtatcta catttatgaa acgtgaattt ccaaactcag
     6361 ggaaggcatg gctgataaat atcgggcagt tttttatttg atatttatta gcagtgtccc
     6421 tcctagttat ctttttattt tatatacaaa aaatccgttt cattcatcgt ttcatcgaat
     6481 acttcgcttt tgattcgatt gaacaattaa ctcagcgttg gaaaaaaaga attcattggc
     6541 tttttgttca taaaagttat tttttagttg cagcattttt ttactgcttt accttcgtgc
     6601 agattttcgt tttagaacca aaagaaggtt ggaaagaaac tatccaggtt gccttaaaaa
     6661 tatttactca gcgacaagca cccatgatcc tgacaatcat cattattatg ggatttttct
     6721 tcttactatt gttgatttcc aatcgttttt ggtacgcctt gactgctacc ttgatcatta
     6781 acttactgct cactatttcg acagtaatca aaatggaaat gcgggaagag cctgtctttc
     6841 cttcagattt aaaaatgctg accggattgt cagaactatt atcgatggtt agcccagtct
     6901 tattaatcgt gggcggattg atccttctcc tattattgat tactagtatt attgtacaac
     6961 gtagactaca aaaacagtat tccttgaaga ttcattggaa acgtcgattt atcagtatcg
     7021 ctgtattatt aggatgtttt tcgggtgtct ttttcattaa tcacaaaaat tcgccttctt
     7081 tcttgctgtt taatttattt aaagtaaaca agactttctt taaccagcaa ggcgccgtaa
     7141 aagataacgg accagtcatt caattcctaa acaatatcga cattaaaatc atggaagaac
     7201 ctgctggcta ttcgaaagaa aaaattaatg gaataatgaa aaagtacaac acaaaggcag
     7261 atgaaatcaa ccaaacaaga agtgactggt taaacaatca gacgatcgtt ctcaatttaa
     7321 gtgaaagctt cagtgatcct gcacgtgtcc ctaatctgac agtacctaca gaccctattc
     7381 cttacatcaa aagcattata acagaaggaa caggaggatt gatgttatcc gtcggttatg
     7441 gtggcggaac agctaacatg gaatgggaag gactgacagg cttgagtatc agtaacctgt
     7501 ctgcttcgtt agttacacct tatacccagc tagtggagcg tcagaaaata agtccaaata
     7561 ttaccgatct attcgatgaa aaaatagcca tccatccttt tacagctgct ctttatcgca
     7621 gaaaacaagt gtttgataaa tttggttttg acaggttcta ttatttagac agtccggata
     7681 aattaactta tacggataaa atagaacaaa gcccacgcat atcggatgat tcagcataca
     7741 aagaaacctt gaaagcttta caaagcaata cggaaacgtc acaattcatc caattatcca
     7801 ctatgcaaaa ccatatgcct tatgatgatt actatagtga attgaactac actgctgaag
     7861 gaactgctgt cattcctaat agaagaaaag aattacaaac ttatatgcaa ggccttcact
     7921 ataccgatac agcagtaaaa aaattcatcc aagaaatcga caaaatcgat aaaccgatta
     7981 cctttgtttt ttatggtgac catttacccg caatctatag tgggaatgat tcgaaaaaat
     8041 atggattaga acagcaccag acagattatt tcatttataa caacaagtat agtcgtgaac
     8101 aagcagctac gttaaataaa gaagttgttg ctcctaataa ctttggtgct atggctcttg
     8161 agcatgcgaa tatcaaagta actccttact atgcgttgtt gacagaagta tctaatcatc
     8221 tacctgcatt aacaattgat cctacagaaa gcttgtctaa tcgttttaat ggaaaacagg
     8281 tctttgttac agaggatagt caaattatcc aagaaaaaaa tttaactgag aaacaaaaag
     8341 aaattctaga agattatcgt ctgatacaat atgatcttgt tgcaggtaac cagtacagtg
     8401 caacatgggc gacacaaaaa ataaaagact aacaaataaa ctgagcaagg ggttgtgata
     8461 aaagacatgt cacaacccct tgtttatctc attaatttgc ttgtccataa acgatttgat
     8521 taaagccatt tttcaattca ttattcgctg tctcgttgaa gtaaagataa ttatcgggat
     8581 tcccctgatt ttgttgtgtc accaatactt ttggctgatt ttgctgaaac ccgaatagat
     8641 agacgtgctt tttcagatat ggttctgtct ctgcatcgga atagacggct gtgatttgat
     8701 agtctgcttg acctgtccca ctttctgacc attctgcaga aacaggcact ccaccaatag
     8761 ccatcttcca ctccccgttc aaaatagctt gagggacttt aagaccatat aagtccacac
     8821 tcatttgatt cgtataactt ttgtattgtt gtcccattgt ttggccccat ttggtcataa
     8881 atgcctctaa ttgtgcggat ttgtctgcat tccacagtgt ttgttcaact tgttcgctgc
     8941 tttctgaaga ggaagatgaa gcctctacag aagaacttga agtagatgtt tgagttgttt
     9001 cagtcgtttt ggaagactct gacgaagtag aggtatctgc tgtttctgta cttgctgcag
     9061 ttgacttgct ctctgtgcta gttgttgtag tagttgcaac tgttgctttc gctgtttctt
     9121 ttgttgattc ttttttcgat tcattcccgt tggtacaacc agctaacaaa aaagctagac
     9181 caattgttac taaaatcctt tttttcaatt aaaaaactcc tttcgattac tcccacttct
     9241 ttattctatc aaataagcaa tgtgatttta ttgatttttc gtattttctc atcaatcttt
     9301 caaatcttct aaaaactgag aagcacccgc tgatgttgtc tgtaatacat cagcgggtgc
     9361 ttcttttatt ttaatcttcg tgattttcca atttacctca cgctttatat ttgtatttga
     9421 agaaacatag acttttagtc atcatcgtct tcctcatcca aatcttgtcg gacagttttt
     9481 ccttggagtc tttgtacttg gtagatgatt tctttattga aacttaaaag ttcaacaagc
     9541 aattgttgca agccatattt ttcttctttt tcagctaatt tgattgcccg tgtgataaag
     9601 acattttgga aatcaaaatc ttttgcaaag ttttccaaca ttgttgaggc tggtaaatat
     9661 ttgttttgac catgttcgta aatttttcca taacgcatga cttcttctaa agtagacgga
     9721 acgatttcat tttcaatcac taactcatga gagattttat tgaaccatct cgcttcttga
     9781 tcagcatatt ctgaaaaaag ggtacttaat tcttgcgaag atgtacccaa aacatacaat
     9841 tgtgcttgga tcaatttcat ttcttctaat ttaaaatttg ctaatacatg ccccaccatg
     9901 gcgccagcag ttggtttatg atggtcgata tctgcttgtt ttgtttcttg aacaaatttt
     9961 tcttccacta tattcattga tttacgcctc ttttactttt gctgattgga cttcataacc
    10021 taatttttcc acaactgcag ctaattgttc agggtggact tcatctggat caaagtttgc
    10081 tttgattttg ctcgcattaa acaaaacttt cgtattgctt accccaggct gttgagaaac
    10141 cgcatgttcg attttttgca tacagcttgg acaagctagt gtatctaatt gcataataat
    10201 ttttttcatc gtagttcttc cttccttttt cttgatagtc ttattctaga ttacagcttc
    10261 cataaaaaaa ttgattctta tcaagtttta cgatttttct tctttgcttt cgtgtccaaa
    10321 atacaataaa cgcatcgcgt taaagataac gatcaagata ctagcttcgt gaacgaacat
    10381 cccactagcc atgtaaataa acccggcaaa taagccgttg agtaaaaata agacgacacc
    10441 aatggcaatc actacatttt cacgggtatt taaaacagtt ttcttagcca agcggtaggc
    10501 atgaaccaac gattcaaaat tagattgcat caagacaacg tcactcgttt cgatagcaac
    10561 atccgttcct gatcccatag caatcccgat atcagccgca gccaaagagg gagaatcatt
    10621 gattccatct ccaataaaag caacgcgcaa tccttgttct tggtattttt tcacaaattg
    10681 aaccttttcg tctggtaata attcagcatg gacttcatca atgcctaaca tatcagctac
    10741 ataatcagca gtcatctgat tatcacctgt cagcatcact aactgttttg ctcctgcttg
    10801 tttcaactta gctaattgtt cagcgacttc tggtcgaatc acatctgaca cacctatgat
    10861 ttgagtgatt tttttgtcga ttgctacaat aatgaccgag ctgcctgttt gttgtaattg
    10921 atgtaaatct tgtacttgct ttggtgtgag agaaagtttg tgagcggcaa tcaatttggt
    10981 gttgcccgca caaactgtat gaccatcaat ctcagctgtg attccttgtc ccttgattgt
    11041 ctgattatct ttgactgcta attgttgaaa atcaatgtct ttctcctgag cataatggat
    11101 cacggcgcgc cccagaggat gatcggataa cgtttctaat cgagccacta aagctaagct
    11161 gtccccttca tcctctgtat agttcttgat agtggataca gcagtagttc ctttggtcaa
    11221 tgtaccagtt ttatcaaaga caaaagtatc gattttggaa aaagtatcca ttgcttcacc
    11281 gcctttgact aagatgcccc gtttcgctcc attaccgatt cctgctacat tcgaaacagg
    11341 cgcaccaatg actaaagcgc caggacaacc aagaaccaaa acagtgattg ctaaacgaaa
    11401 atcacgacta atcagaccaa caaccaacgc aataagtaaa acggttggag tataatactg
    11461 agaaaatcga tcaatgaatt tttccgcatg ggatttggta tcttgtgctt cttctactaa
    11521 ttcgataatt ttggcaaagg tcgtatcatc tccgacctta gttgcttcaa tcgtcacgta
    11581 gccatctgct aaaatcgttc cagagaagac ttcatctcca ctgattttga cgacttgtcg
    11641 tgattcacca gtcactgcgg cttcatccgc ataccctttc ccgtcaatga ctcgtccgtc
    11701 cacaggaata gctccaccag tttttactaa gacgatatcg ccttcttcca cgtcatcgat
    11761 ttccacttct tcagtcgttc catcttcttg gacaacacta gcagtagatg gggccatctc
    11821 ggtcaaagcc ttgattgact cccgcgtttt ggtcaatgtt ttttgttcca gatagctacc
    11881 aaacaaaaac aagaatgtaa cgatagctga ctccttgtat tctccgatca taaaggcacc
    11941 aagcacagca atagtaacga gtaaatcaat tgagataact ttgactttca ctgcctgata
    12001 cgcatggatc ataataggta tgaccgcaat aatagaagct atggctagag caatgttata
    12061 gaacaagaca aaaccagtaa attttccgat aaaaccaaaa ataatcaaca cagctgataa
    12121 ggcggtgata tgatttcgat gctcttggat ccacttttgt aatttcatct ttctccctct
    12181 tttcctttca ttcttacgag actaagtata gatacttatc catcataaaa aattgaccac
    12241 tatcaagttt agtcaaaaag gcggttttct tttggtagaa tacactacaa aaaatagtta
    12301 gcaagaagac tcactaaaag tgatcttgcc aactattgtc gtttctatcg aaattttcat
    12361 tgaatgattt tcctgtacag catacatcgg agatctattt ctttatctaa gatttttatc
    12421 ttgctaattt tttcctttag tctttcaaat ccacgtcgtt catagaattg atacgtacag
    12481 ccactatctg taaaaaggaa tatttctttt cctttctctc ttttttcaaa ttctgttagc
    12541 aacttggtac cgatgccttt tgttttcaaa tcaggatttg cagctaaaaa aatgatctct
    12601 ccatctggcg aatgcttctt taaatatgct gcaagcattt gttcatttgc tgtctcgtaa
    12661 gctcctttac tttctttagc aaatatattt tgtaaaaaat cgaatatctt aacatatatt
    12721 tgctgtccaa aagaacgatg tttctttttt tctcctttta tctccgccag caaaacacct
    12781 gccaactcgt cctctgcata caaagcaaga atttgagttg ctcgattcaa ctccatatac
    12841 aaaaaatatc ttccgtaaag attcagcaga agttcatttt ccatatatct ctcaaagttc
    12901 attccttcaa cagcaaaata gatagctttt ttgtaatctt tcttttctaa gggtttaaaa
    12961 gttagtttca tcatttcgcc tcctcaactt caatgattgt gttatactta cattataata
    13021 gacacagtgt ctactatcta taatcaatca tctataagtt tgtcgattaa gcgacagctt
    13081 tgctttgata tgtcggataa ggagataggt aaatgaaaaa gacagataga cgcgtcaaaa
    13141 aaacagaaaa agctttggct gaaacactat ccacattatt agtaaataaa aagattcaag
    13201 caatcactat acgcgaacta acagaaacgg cagatgtaca tcgttcgact ttttatacac
    13261 attataagga tatctatgat ctatatgatc aattagaaag caactttttt cgggatctaa
    13321 atgaaattct ctcttatgat ccagcccata gctatgagga actatacact cgcttgattg
    13381 actatgttta cgcaaatact tttttatatc gcttatttat cctcaaccat ggtgatattt
    13441 cgtttcagaa gaaagttaaa caagttattg aagaaaacta tctgaacata tggctatatg
    13501 aagacaagaa aacgattatc acagaagaaa tgagattctt tactacgtac cacgtccaag
    13561 gttgtctatc cgttctcaat cggtgggtag aaagcaatta caagtactcc aaaaaagaat
    13621 tattagaact cttacagaaa ctgaatgatc acatagagaa aatcatgcct tgaataaaaa
    13681 catactaagc ttagttaaga agaaccgtaa ccttctcgta taacaactga atcttattta
    13741 gccaaattgt ttactgaaga ttttttgagt aaaatttcta taattcctac ttattgaaaa
    13801 gtagtttgta gtgctgcttc tccctctcta agtgatatat cttgttgtac caatcaaatt
    13861 cacctcatca ttaataaatt acttttaggc taatttatta caacttatag gttgaagctg
    13921 taaccaattt atagtcatat tatattttta tcatccagtt tattgaattc ttctcgttta
    13981 cctcaattca ttttcaaggg gaatcagctc atcatctaac attttacgcc atgctcgctt
    14041 catttcttgt gggtatttaa atggtttcgc ttctggatgt ttggctttat atccttcgta
    14101 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
    14161 cagattagaa gcatgtaatt ttttctcaga aacaaaccac tcattggaaa agacatgaat
    14221 caaatcatca cttacttgtg taaaatagcg acgtttaatg acaaagatat cttcattgct
    14281 atctagtgta ccttcatttc tttgtgctta aattcaaaga taattttctc aataaagatt
    14341 tttgattcca gtaagattcc tttactcctt ttacaacata gcgttgtcct ttgttttgaa
    14401 acatcttctt cccctctttg ccattaaaaa tagcccaatt ctctataaag aagattaagc
    14461 ttcattatta taatataaag tttaaatttg gtttattact tactttttct tattctctag
    14521 taagctaata ttgaaaaaaa tgtaatcaga tcattatttt tatatgttgt aacttcctga
    14581 taagaaaact aattaaaaga aagaggtaac tacataaact aattaaaaaa gctaacttca
    14641 tagttggagg ataaatggaa gaaacttcaa tgattgtttc tccttgtgtt ccttcaatcg
    14701 acactagtcc tattatcaat ctctcttttt tgtccattta cttttgcgac tgttccccaa
    14761 taatcaaata caggaagcgt tattttttgt cctttagatt catgtactgt atagactatt
    14821 ttcgaatcca tcacttgata aggcatagta tgccattcct tgccaattct tacttcatga
    14881 ttctgcaaat gttctttgta ttgtaatgct tccgtgggtg catagtcata aagtcctccg
    14941 ggaaggagtg tcgtgtaacg aataagattt tcttgattca ttaccaagtg atctctctga
    15001 gtgacaactt tatgttcata gtttgctttt gttgcagcag atacagccaa cagtaataaa
    15061 acaattgccg ttatattaac tacttttctt tgcttagctg atgtgctctt catgaaaacg
    15121 aacgataaaa tcaacgagcc aaacagtact tgaaagccta agattcgcca aggaaattga
    15181 atcatttgaa tgggtgtatt ttgtaataaa gaccatggaa aaaatttggt ggataagagc
    15241 caagagacta ccgcactaag gtaaatcact ttttctttgc gattaaatcc tttaaaaaag
    15301 aagagtggac agatcaaaga aataatacca atgattccta ccgtaaaggt tgtcaggtca
    15361 ttattcatcg caccaatcaa actatccgag acatttaatg cttggtgttg taaggtcgtt
    15421 ttatctggct cattaatcga ttgaaccatg atttgtcgga acatcggata ccaaaagtat
    15481 gctgttaaaa gaatcgtcat tcctactgcc tttgacaact ccacaatcaa ccggatgctt
    15541 tctttttttc ttgtttcact tactacaaag ccgcctaaga ctagtaagcc aacggctatt
    15601 acactcataa aagtggaaag aacatgtgta taaatgagta agctcatact gactgctaat
    15661 ggaattgcac atggcttttt ctcataaatc agtgcgtaag cataatacat aactatcggt
    15721 ataaccgtaa gtgcaatcgc ttcagcaata gcagaacgat gataaatatc aatcgtacgg
    15781 tacccataaa atgtatataa acagctaaat ataaaagccg catcgattct ttttaaaaat
    15841 tttagtccat aatgaaacat cagcatgata gtacaaacat tcaatagcca aacatataag
    15901 atataactaa cgattaaatt attacttatc caataaaaaa tgactgccgg aaaaaaggtc
    15961 aagtagggat aaaaataatt cactccgctc ccataatgat taaacgtcgt aaaattgatt
    16021 ggaccagaaa aaattgaaga taaccctttg actctagcaa aatggaaggc aatatccccc
    16081 gatgtagaaa tagtatgaac atttctaaaa taattgattg aataaagtcc agcaataatt
    16141 gctaggagaa tggacaatat tatttttttc tttacttgca ttacttaatt atatactcct
    16201 ttcatggatt gctttatcta gtataactta aattttaaat aaatcgttat ttatttttac
    16261 tctcacttaa aaaagcacta aaattataag agagggaggt aatggtctga acaccaagaa
    16321 ttgttccaac atcgaattgc ttaactaatg agttcaaagc ttaattatgt ggctcttata
    16381 tttaccgagc gtggattgcg atctctttag cgaaacaaac atctttcata aaaaaaacag
    16441 ccatatccgc tttcttggat atgactattt actactactt tactatcttt cattcttctt
    16501 gtctcactta tgataaggcg ctgcgtatca tgagtgaatg atattaggaa aacttcataa
    16561 aataaatctt ttatacattt ccacttctag atggtcattc atgttagaag aatcaaacta
    16621 tttttttact gtcgtaataa tattaaaaca ctagaacaaa tcaattaaat tccataaaat
    16681 ataaataata gacctagcat catctggtat aaaatggatg ctaggtccgt tatatttact
    16741 caaaattgat tctacgaata gatcttctta tcatttctat tttagtagtc atgcgttgtt
    16801 gttcttctaa aggtggtaat ggtataagta gtttagataa atttgtacca tttacatttg
    16861 gttgcccagt tccgtaagaa attttttcta ataacttcca atataatggc gagtctaaga
    16921 agcagtctac gtattctgat aataatttct caactaacct aattcgaatc aagtaagaag
    16981 caaaaacaga ttcctcactt atttccttaa ccaagaaact ttttcccata gttcctcctg
    17041 tccttgctat caaaatgtca ttttcttcca atcttaaatc aactagttta gaattactaa
    17101 tatcacagta aggcacactg gaccaattta cacgtccctc ttgtatatct gtgattctca
    17161 aaaacttaac attgccttgt tttttcgccg aatctgtata gccatattga atcttagtag
    17221 aaatacttcc taaataaacc catagtcgaa catcgctaga tgatttttct tgtcgttctt
    17281 tatgaacaac agtgatcatc ttgagttgtc cgactatttt ttagttgcag caatcttttt
    17341 gggacgctta tttgcatgaa gcaacgttgc taagtctagt aataaaacat tggctaacac
    17401 aggattggct ttctcgcgat acgctgcggc gccactttca aacaatactg ctagacgatc
    17461 ttctacaaca acgatttgtt ccaaatacgg tggaaatttg attcgtttta ggatccggct
    17521 aggagagtcc cgctcggaca ctaagagttc cgaggtataa ggaccataag aacgactggc
    17581 taagagataa tggccataaa aagccacacc ttgaatctta tcagatgttt ttctgctcat
    17641 tgtcacggtt ggttggattt tgtctttgcg gcgaattgct gttttcattt cagctatcgt
    17701 cggcaataga tagaaagtca actgtccttc accttttagt gtgaagttgc cagataccag
    17761 ttgatcattg ttttgagtca aatacgatgc ttgtggaatc tctgagagat cagttgtcag
    17821 ccaataagcg attggctgtc ccgtctcttc agatgtatct gctaagatat cgatcaaacg
    17881 taaagcggct actcgtccag tagcagatcc tgtgattgtc aaccataaaa gttcccgttt
    17941 tgtatcatat actaagcccc ctacatgtgg ccgtttaggt aaaatcagca gtttgatcgg
    18001 ctgtcctgtt tctctatcca gtacatgaat aatagaatga tgctgatggt catagcagta
    18061 ggcagaaata aacacaaaat cttgagaaaa agccaaccct tgcggcgtca tccattgaca
    18121 aacctcattt tcgccggttt ccggcaccac cattcttgtt tctgttaatc caggaatgtt
    18181 atattgtttt gcttcagcct tttcccaagc tgcttgaaca ataggatacc gttttaactt
    18241 tttttcaata tcctgatgta tccgttgctg cttccaatgc ttaaacttta ggacatagta
    18301 aacaagaagg ccaatgacca gcaacactaa aatcattatt gctacccgct gtgtcatttt
    18361 tttgcctcct tgttctattc gatcaatcgt ccatataccg ctattaatta ccgtaactac
    18421 atgtttcttt tactagcgtt tctttatagg atctttcata atctcattta tagtaacatt
    18481 aagaatccct tattctacta tagtcacttc cacatcaata ttgcctcttg ttgctttaga
    18541 gtatgggcaa aatgcgtggg cttcttctgc gattttctct gcttggtcct tctcaagtcc
    18601 tggcaaagaa acttctaaaa cgaccccgat ttggtagccc ccatcttcta attgaaataa
    18661 agatacccgt gctttgactt cagaatcaga ctcaagatta ttttcttcca gcatgtgttg
    18721 aaccgcgccg ttgaagcaag atgcgtatcc agccgcaaaa agttgttccg gatttgtccc
    18781 ttcactatgg attcctgggc ggtcgatctt catgtgcata cttccgtttg gtgcctcaac
    18841 ttcgccatca cggcctccac gattgatcat tgctgtttcg tacatttttt tcattgagat
    18901 catcctttct ttcgtcgttc tatattaagt gtaacgagta ttttttaatc tgacaaaggt
    18961 aatgcttgca ttcggcgaca tcagttagac aacatcgatg atcagtgata gaagcagttt
    19021 atctatacaa aaaaagaagg gttctatgat atttggtgtc cttactcgcc ttgagtaaca
    19081 acaccaaata tcatagaacc acttaatcag cttcacactg ctttttctac tttcgtagtt
    19141 gaaaaaaact taaacaaatc atcggcaaat agttaaagaa aaaacttaat cgttgaaaga
    19201 agcccaattg cttaaagaag gcaaactgac taatcgacgg aatccgtgct aatccgtaaa
    19261 gacctgcaat tagtaaacta ataactgtta aaacaagata aaaatggcta agcgtacctt
    19321 gaccactttg acgataaagc agaacaagta acaatgggaa aagtagaaag cctgcatagc
    19381 ctagcccaga acctatatta tggatccaag tcgatagatt acaagagctt tcattcgtat
    19441 tgatactgaa taagccagta aaaatacaat cacctatccc atataaacca actgttgcaa
    19501 caacaagtat tgcaagtgtt tttgatgttc caacaaatag gtgatacaac gcaggcaacg
    19561 aaagaacaaa aaaaagtcca gatacaactg accagaccaa gaaagcacgt ctgactggac
    19621 tatctacgtc accaaacaca ctaattacgg tagtcatttg attcagtttg ggataaaaaa
    19681 ggccaaggat atacggtgtc gataaatcac tcatcacacc taataataaa aaataaaaac
    19741 catatttttg caaaaaaatc attttcttcc cctacttttt agtgattatt cgatacatcc
    19801 aacaatcaaa ttttttctca ttttaacaca tatttttatt ctagttgtct tttgaacaat
    19861 caataacata aaggtgagtt tcttctacat tttaaaaaat cgaatggcac tgaagagttc
    19921 agttgtcagc atcaatctcc tactcaaaaa ggcaaaaaaa cgtaagtcag atgttgttga
    19981 catctcactt acgcatccat tgattgaaaa tttcttagta aaatctacta gcctaaaaat
    20041 ttgaagactt cttctcgatc atacagttta aatactttct cgcataaaaa ttgagtaatt
    20101 agtgccgcaa aaacagggat gactaaccaa caaagaacaa caagaaagcc atttagccct
    20161 gcatctaaag aagccaacgg accaacaagt ccgaccacgc cgaaaccagc agaagcaggc
    20221 gtacctgaaa tagaaaatag agcgacagga atagctgtaa tcactgcact aactaaacaa
    20281 ggaataagaa tcacaggttt tctaaataga ttaggcatca tcatcttcat agcgcctaaa
    20341 gcaatcgcaa ttgtcacacc tgacttatta accttccaag agttcactac taaaacaaaa
    20401 gtggttgcag ctacgcccat cgcagccgca ccagcagaaa caccatctag ttggattgcc
    20461 attccgattg ctactgttga aataggagag ataatcaaaa aggcaaagga acaaccaatc
    20521 aagatagaca ttaacagcgg ttgtaaggta gtgaagttat taatcaatga tccaattgca
    20581 gaagtaattg ctgttacaaa agggaataaa taaaaaccaa gtaaacctgc acctacacct
    20641 acaacaatcg gtgttaacac aatcgacact gacccaaaac tatttccaat cagtaaaatc
    20701 agcccgacag caatcgatgc tgtcagcatc gtattaataa tatcgcctgt accagcacca
    20761 acgtaaacac ccgtttcacc caaatcaggt gtgaatttga ctacaccaga accaacaaaa
    20821 gaagctccgg ctacaaccat cattctcatc ggatcaaatc caaattgtaa ggcaatcaaa
    20881 ccaccaatga tcaaaggtgt tgctagttga aaaataactg ctaactgtgc gatcgttacc
    20941 gcaaagggat acgcactaaa atattttaaa atggcaccta atactgcatt aggaatcaat
    21001 ccaataatga ttcctgttgc tgtaccagcg aggactttat tcagaaaaat tttcggtgat
    21061 attttttgtg aagtttccat tcaatctctc ctttatttta cgttcaaact atcttctttt
    21121 gcatggacaa gctcagtcaa gaaatcacaa taaggcgtcg caacaccata tttcttccct
    21181 tttctagaaa ttgcgccatt gatataatcg atttctgtta atcgatggtt cttaatcaag
    21241 tcttgataca ttgaaggata atgtaaccca attgtttccg ggtcaaaaca agattcacaa
    21301 tgtgcaatga cttctgggac atctagttca atcttctcta ctgctgctac tttggcaaac
    21361 tcattgacaa tcgtcgccac cattttatga gcagtcgatg tttttcctag ctcagccata
    21421 ttaacgtcta agattgtgca tagtccgttc attgttccgt tcacacatgc tttgcgataa
    21481 atagaatagt gaatattgtc agaaaaatga gcatttaatc ctgattcaga caatttgtct
    21541 gctaattttt ttgcagctgc ttcttttcca tcacctaaat tttgtaattc tactgaacca
    21601 cttccaaata atttgacctg accaggacct tcaagacctg ccgtccacat ggtatttcca
    21661 atatagatat tttccattgg tacaaatttc tcaataatat cttcatgccc gataccattt
    21721 aatagacata agacctctgt atcttttttg attaatgatt ggatatcctg cagcattttt
    21781 tccagctgca tcgctttggt aaatagaata atcagatcaa cttgatcttc tttttctact
    21841 tcggattgaa gaacgattgg tagttttgct tctacctctt taccattaaa atttgcttgt
    21901 aatccatgtt ctttgatttg ttggacatgt tctgcccagc catcgattaa taatacttcg
    21961 ttcccacttt ggtgaagcat caacccaaag cgacttccca ttgcgccagc gcctgcaatt
    22021 gctattttca ttgattcttc ctcctttttc tttctattat actcatttga gaatcttttc
    22081 acaacctcgt tttgttattc ttctcttatt tatcatgttt atatccacca ttcaagttat
    22141 tttatcagca aatcaataaa aactggaata gaatcccaat ttcatcgtaa gagaaactag
    22201 actagcaata agaaaataga aacattcata gaaaaaatct cactcctctg attataaaag
    22261 agggggcatt aacctcgtgt atactaaaac cttccattca ttcgatggat gattttcttt
    22321 ttgtgaaaaa atattcattg tagtacgatg ttgcgcaaca cagacggatg gtattgctta
    22381 ttatttatta aaatcgaaca atacacttat ttaaaaactt ctttcccaga ttttcttcca
    22441 tttttacagt tataactagt attatcatga aaaatagcct tttattgtct atgggaataa
    22501 taatttaaaa ttggtacttt tttattaatt agttcatcgc ccaataatct ctccagtttc
    22561 ttagcatctc ctgactcaac cggaattatc caagcccagt ctaaataatt ttttaaaatc
    22621 tcatcatatt ttgcaaatat ttcattgcca tattccgtcc ttaaaattat attttcagtg
    22681 ccagacaagt gatttatatt gagttgataa aaaagatctc catctaaaaa aatatcatta
    22741 gaagaagatt tccccacagt aatgaccatt ccatcagctc tatgtgttac atacaaataa
    22801 ttgtatgacc tcatttcacc taatgaatca attatttctt gccctttagc tctaatatat
    22861 tgatctctat tacctatctc actcttattt aacaaaagtt ttttcatgtc aaatttaatc
    22921 ggctgtttta tttttgtaaa ttcgcttcta ctactaatcc aatccgagat taatttttca
    22981 acatgtccca gagtaaactc tgatttattc gctaaatact ttttttgttg tattaaagac
    23041 atctttcccc gaagctcaaa attgagccta catcgattct ctatctcttc aaaaagcatc
    23101 ttatcttcat cattcttata ttcatcattc ataaaaccaa tccagctcct cgattaagat
    23161 aatattttaa tctcatacaa aaactagctc tcatcaaaac aaccattgtt ttgatttctc
    23221 atgaaatttc tttatacttt tctccgatta taaattccta accccttgag aaaattacgc
    23281 atatatttat ctccgcaagg cttaaaattt cgttggcctt cttttctcaa aaatgccccg
    23341 atttcgcctt ttgatggata gatttctgcc tcagcaaaaa cgtctaaaat atcatcagtg
    23401 gtcatagaca aggcgatttt taattttttt agcaacacat tgttgatata tttgatttcc
    23461 ttgctttttt gcggcgtgtc tactatcggc ttatcttttt gcggaccacg cgccaagaga
    23521 atcaggccat ttaggaatgc gtcaaaaact tggttgttga tggttttttc atacacatta
    23581 ttttctgcat tatctttgtt cttatcctgt actttggtca gtaccgcctg agcttcttct
    23641 ttagtcagtg gtacaccgcc tagctcaaaa gctttaatca aatccgtatc tttcaaatca
    23701 atcgcataac gcaatcgcaa taacctatca ttgttattca tttgttcctc cgaagtattt
    23761 agtctttact ttcttacgta ctttttgtct tgctaagatt tttaggatca atgataaaaa
    23821 ccgcatcact tatgataagg cgcccccgca ttgatccgga aggcacggta gatttgctcg
    23881 acaaggatca gacgcatcaa ttgatgtgga tacgtcattt ttccaaaaga gatctgagca
    23941 ttgcttcttt tcatgacggc ttcgctgagt cctaaagagc cgccgataac aaaaacaagg
    24001 tggctttttc cttggatacc taattggtcg atttgtttag caaaagattc acttgtagga
    24061 tttttccctt caattgctaa ggcatagacg tattcttgtt ctttgatttt cgctaagatc
    24121 cgttcccctt ctttttcttt tacttgtctc atttgcgctt cgcttaaatt ctccggggct
    24181 ttttcgtcag gaacttcgat caattcgatt tttgcatagg cattcagccg tttgacatac
    24241 tcattgatac cttggatcag gtatttttct tttaattttc ctactgagat aattttgata
    24301 ttcatgtttt ctctcctttg gtcttcatgc tcactttgtt tattataatc aattttttcg
    24361 tctatatgtt tattttggct atttttgtta aaaagatttc cccaacagtt atgcacaaca
    24421 tccacaggtt tatccacaga aaacagtgtt attttacctt atttcatcac attcattagg
    24481 caaacatacg ttttataaga atttatccac tttatacaca ggctgtggat atgttttatc
    24541 ccttattttt ccaatcctat ccgcgtagtt ttacacagta tctgtggaaa aagaaaatca
    24601 aatattttca tctgaaaaca atgatttttc ctaaagtttt tcataaaaag ttcacagtct
    24661 caaagtagac taaagtcaac aaataagact atactaacag tagataaatg aatgtaattg
    24721 gaggaggaac acatggatag aaaaaatgtg acaccaaaga tgaaaaaaag taaaaataat
    24781 agcctctggc gtaagttagg tctggggctt gttggaggga tcgttggagg actactgaca
    24841 gctggcattt tttatgcagc gatgggcact ggcaacaata gttctactgc tacaagcggc
    24901 aatcagaatt ctgccggtga aacagttgtg gaaaatgtca aagtcaatgt agattctgat
    24961 atcaccaaag cggtagataa agttcaagac gctgtcgttt ctgttatcaa cttacaaagt
    25021 caaaaccaat caagcggttt tggcggacta ttcgggcagc aagaagaaag tagcagctca
    25081 gatgatagta atttggaagc ttatagtgaa ggaagcggcg tgatctacaa aaaagacggg
    25141 aatactgctt atgtagtcac gaataatcac gtagtagatg gccagcaagg tcttgaagtc
    25201 ttgatgaaag acggtacgaa ggtaaaagct gaattagttg gaacagatgc ttattcagat
    25261 ttagcggtgt tgaagatcaa ttcagacaaa gtcgaaacag ttgcctcttt tggtgactca
    25321 agtgctctga aggttggtga gcctgcgatt gcgattggtt ccccactagg atctgaatat
    25381 gcaaattctg tcacttcagg aatcatttct tccttgaacc gacaagtgac aagtacgaat
    25441 gaatcgaacg aaacagtcaa tatcaatgcg atccaaacag atgcagcaat caaccctgga
    25501 aattctggtg gtcctttagt taatatcgaa ggacaagtta tcggtatcaa ctcaagtaag
    25561 atcgcaagta cttctgaatc tacttctaat gtaagcgtag aaggaatggg atttgctatt
    25621 ccaagtaacg atgtagtgaa tatcatcaat caactggaaa aagacggaaa agtgacacgt
    25681 ccagctctag gtatcacaat ggtcgactta tctgccgttt ctactcagca acaagaacaa
    25741 atcttgaaga ttccttcatc tgtaactaac ggtgtcatcg tgacaaatgt ccaaacagct
    25801 acaccggctg aaaaagctgg attgaaacaa tacgacgtca ttactaagat cgatgacaaa
    25861 gaagtttctt ctggtgtaga acttcaatct gctttgtata agaaaaaagt gggagattct
    25921 gtcaaaatca cttactatga aggctcgaaa gaaaaaacaa ctactgttga attgacgatc
    25981 gatcaatcgg cattaaaaca aaatcaaaat agtgaaaatt aataaaaaag aggttgtgac
    26041 aaagacttgt cacaacctct ttattttgga aatgcttctc cgactacggc atcctcaggc
    26101 aatactaaga ttcctttttt tgctggagca tttggcaaat gcaattcacg agcggaacag
    26161 atcattccat agctatctac ccctcgcaaa gttcctggcc agatcatcat tccatctggc
    26221 atcatagctc ctggttttgc aacgacaact ttttgtccag cttcgatatt gggtgcaccg
    26281 caaacgattt gcaatactgt accattgtct acttctgtct cagtaatcga taaatggtca
    26341 gaatcaggat gtgggacgca tgtttttacg tacccgacaa cgatcttcgg ttcatgatca
    26401 gcttcaagct gttcttcgaa tcctgcttct ttgatcaatc gattcaattt cgctaattcc
    26461 tcatctgtta agcttacttg gccgttccct tcgattgtta gatgatcgga gatatggaaa
    26521 aagttccaag caacaacttg accttgttca tctttgatcc gagcaacatt ccatttgcgt
    26581 tcaacagtgt tttctgttcc tttatcgtcg gctataataa ccatcaaggt gtctccgacg
    26641 tgtgctttgt tataagcaaa aatcatgttc tttcctccat tatcctttgc tgagatttat
    26701 cttaacaaaa aattgcattc actgcatgaa atttttttca tgctttattt ccgaagaagc
    26761 ctcttctgga acaccattta ttcgtaacaa gggactgtga caagactttt gtcacagtcc
    26821 ctttactgtt ttagttaatt gattaaaaag gtcgtacaag ttccacttgc ttaattactt
    26881 ggctgttctt cttcgtcaga aacgatcaag acatcacaag gtgaatgacg tatcacgtag
    26941 ctggctacac ttcctgtcat aaagcgttcg actgcattta gacctgactg cccaaccatg
    27001 attagatcga tctcatattt ttttggcaat ttgactgcca tggattcacg aacagaacca
    27061 tatgcaataa ttccttctat atttgtgtaa tcaacagatt tcccatattc tttacattct
    27121 tcgatcattt ccttggctgt ttcagtttgc tgatcgacta gattttgatc aataggtgca
    27181 tagcccataa agttataaaa ttgctgatcg attacgtttg ctacatatac tttcccatta
    27241 ttacggcgag ccacttctac cgcttttttg aaagcagcaa gtgcttgttc actaccgtcg
    27301 atacctacta aaatattctt atattcttga ttcatcttga cccctcctaa cagatgtcag
    27361 ccttttgtta cactttctag gaacgattcg atttcctgtt ttgttttacg atctttattc
    27421 actagacgac ctaattcttt tccgtcttgg atcacgacaa aacttgggat accaaaaatg
    27481 ttccattcag cggctacatc tatgtacttg tcacggtcta cttcaataaa ttgccaatca
    27541 gaaaagtcat tttcaatttc aggcatttgc ggtttgataa aacggcagtc tccgcaccag
    27601 ccggcagtaa agaaaaatac actctttcca gtttctacgt agcctgctaa ttcttctaaa
    27661 ctatttggga taatcattct ttgtcctcta ctttctgttt atgtattttt caattaaatt
    27721 atacaccttt ctttcgaact aacaaacatt gatcctcttt ttataaaaaa aatctggcaa
    27781 aaagcaatct tttgccagat tttatagttt cactatcgca ttattttccg tatacgatcg
    27841 tatttactgt tgatttatcc aaacctttca gtgtttgaat caacatttct cgtgcagctt
    27901 caaaatcgcg aatactgaac atcgtttgat gcgtatggat ataacgtccc actacgccaa
    27961 tcactgtact tgggattcct tcgttttgtg tatgagcagc acctgcatct gtaccgcctt
    28021 ttgaaacaaa gtattgataa gggatattgt ttgtttcagc gatatccagc aagtactcgc
    28081 gcaaacgagg aagcatgatc aaacctggat cttggatacg catcaatgta ccttctccta
    28141 aatgtccgaa tgtgccattt ttcgtcactg tatcatctgc tgcagagcag tctactgcaa
    28201 agaacagatc tggcttgaat ttattgacag aagctttaga accacgcaag cctacttctt
    28261 cctggacatt cgcgcctgca atcaatgtat gacctaatgt ttcattttgc aacgcttcta
    28321 aagcttctaa taccattgta cagccatagc ggttgtccca tgatttactg ataatattct
    28381 ttccgttggc tgttttgatc gtttcagcgt aaggaacgat tgtgtctcct ggaaggacac
    28441 cgtaactcat tgcctcttct cttgattcaa acccagcatc gaacaaaata tcggttactt
    28501 ctactgattt ttgtccgctt gttcctcgca ataaatgtgg gggaacggaa gaagaaatac
    28561 atggataatt cttaccagta gaggttttaa gtgtaaaacg ctgagcagaa acaacgtaag
    28621 gattccagcc gcctagaggt acgactttaa aaagcccgtt ttcttggatt tgtgtcagca
    28681 tgaatccgac ttcatccata tgtgctgcga ccattacacg gggtgcatcc gcttcttcat
    28741 gatggcgcag accaaaaatg ccgcccaaac catctaactg taactcatct accagcggtt
    28801 ccatatgact ttccatataa gcgcgaacat cttgttcaaa accgcttgtt ccttgtaatt
    28861 ctgttaactc tttgattcgt tgaaatgttt tttcttccat atgctattcg tctaaatttc
    28921 agactaaact cctctcagac ttatattgat ctgcggataa aatatgattt tttctgcaga
    28981 ccgatcgtat ttattatagc ttattttttc tggtagcggt tatttatttc aaagaaaata
    29041 tgagagtgtg gtaaaataag cacaaggagt aacaagtgag aagaaggagg tatgtccaca
    29101 tgtatgaaga aagcgaacta agctatttta aaggcgggtt agcagttgga atcggtctag
    29161 gattattgag cggggtagct tcaacgattt ggtatcatcg caaaaaagta ttgtcagcag
    29221 accaagtact tgaaaatatc aagtcatctt ttctaaaaga aggtgcaatc gaaggttctt
    29281 ggatctcttt tgaaaaggaa cctattagga aattcgccgt tcggtcgagt gcttatcggg
    29341 gaggtctttc tcgaatagaa gatggcgaaa ctgtttatta tgaattttta gcagatgctc
    29401 atacaggaac cgtattagaa atttcaagaa aaaaaggtgc aacagattaa tacgtcaaaa
    29461 aagtgagaga acgaatacgc acccactatt caaaagagag gtatcatgtc cttcttttgt
    29521 agtgggtggt tttttagata gagctttcca aacatatatt ccagtatttt ctttttaaat
    29581 aaatgatatt tagcgggaaa aactagcatt tcctttgatt atgaggtatt tttaatttag
    29641 cttttgtttt ctatagttaa ggagagtgaa aaatggaaca acaagaaaaa attgctgttc
    29701 tgattccttg ttacaatgag gaagcaacaa tttcaacagt cattgcagac tttaaacgag
    29761 aattaccaga agcagatatt tatgtctatg ataataattc gaccgatcga acatacgaat
    29821 tggccgttca aggtggagca atcgtaaaaa aagaacctcg tcaaggaaaa ggaaatgtga
    29881 tccgccaaat gttctttgat atcgatgccg actattattt aatggtagat ggcgatgata
    29941 cttatcctgc agaagctgta catggtttgc tcgagaaatt acgttctggt gaagcagata
    30001 tggtcattgg tgatcgccta tcaaatggaa cctactttga tgagaataag agacctttcc
    30061 acgattttgg aaataacctt gttagaaata caattactag aatgtataaa acaaaaattc
    30121 ttgatgtcat gaccggctat cgaggattca atcgtatctt tgtcaaaagt tttcctatta
    30181 tgagttctgg ttttcaaatc gaaacggaat tgacgatcca tgctttagat aaaaaattca
    30241 agttcgttga aatcccaatc gattatcgtg atcgtcctga aggcagcgaa tcaaaattga
    30301 atacattctc tgacggattc aaagtgatca tgatgattat taaaatgttt aaagattata
    30361 agcctttgct tttctttggg atttggacca tcttcttctt tttatttgga ttggtcacgg
    30421 ggattcctgt gattcgcgaa tatatgctga caagctttat tacacgcatc ccttctgcga
    30481 ttttaagtac tggattgatg actttagcac ttctttcatt agtaacaggg ttgattttgg
    30541 atacagtcgt cacgaacgct aagaaagaat atgaattgaa tctatatcat gtttatcatg
    30601 aataccacag tagaaagtaa aaaaaggatc aagactgcaa aaataatttg caattcttga
    30661 tcctcttttt aattaattat ttcgccaaac gatagatggc atctgcatag attgctgttg
    30721 ctctgaacaa gtcatcaaga ctcataaatt cgtttgcttg atgcattgta tcagtatatc
    30781 ctgggaacat cgctccataa gcaacgccac gtttcaacaa acgtccgtaa gttccgccac
    30841 cgatgatttg ttcgtagcct ttttctccag tgtggtcttc atagacttgc aataatgtag
    30901 ccaccaatgg atcatccatt ggtacgtaat gtggttccat tacgcgagca cctcgagtaa
    30961 ctgttgcgcc ttcttgcccc actgtttgtt cgatgtcact ttgcaatccg tctactgtta
    31021 caccttttgg aaaacggaaa ttcatattga tgaagttcgc ttcctctgta ccattttctt
    31081 caaaagcaaa tagtccagca ttcattgtca atttgcccat tttttcatct tcataagcga
    31141 ctcctagttt ttctccataa aaatcttcat ggacataagc agctgctaca tggataaatt
    31201 gtttagcaga tcctaggaat tcatagttat ctaagaacaa tgccaagaaa gaacctgcat
    31261 tgataccaga ttgtgggctt gcaccatgtg cgcctttacc aactaattcg atttttacat
    31321 acgtattgtc cgcttcaatc gttccgctta ctggatttgc ttcgatgaat tggtaaaatg
    31381 cttcttccat cgcgatagct gcatctgcac ttggtacttg aagtgctgca gttgctgttc
    31441 ctggcaccat attttcgcga agtccggaaa caaaagattt caagacataa tctcctgcat
    31501 tgtcgccttt gaagcgtaaa gctaaagaaa catttccttt ttcaccattg atgattggga
    31561 attcagcatc tggtgagaaa ccaaaatctg gttcttcttc atgttcgaaa taataagcca
    31621 tatcgcccca gccgctttct tcatcgctgc cgactacgaa gcgaactttt tttgatagtt
    31681 tcaagtcaag ttctttaatg atcttcatcg catagtaagc tgccatactt ggacctttgt
    31741 catcgcttga tccgcgtgca aagatctttc catccttgat tactggttca taaggatctg
    31801 tgtcccagcc atctcctgca ggaacgacat ccatgtgtcc aaatatccct agcgtttcgt
    31861 ctccttcgcc aagatcaata tgccctgcat agttatcgac attttttact ttaaatccat
    31921 cacgttcgcc ataagctaac atatgtttca atgcgtcgcg aggtccaggt ccaaatggtg
    31981 catctggtgt tacttttgag tcatcgcgct cgctgttgac acgtaaaaga tttttcaagt
    32041 cttcaagcaa gtcttcttta cgtgcttcta cttctttttg ccaatcaatt gtcataaagt
    32101 taatcctcca cttatttaag tcttcgtgtt catgatacca caaattacag aaaaaaatag
    32161 ttcaaaaaat ctgacaattc cttttcttat ctgtttttct ctaaaaaaaa tatacgctat
    32221 aatgagtaaa aggaggtttt tagatgctga taaacgaaca actagtgaaa agagtcatca
    32281 cacctcgtcc aaaaatttca cataaaggaa catttggacg tatcgttttg attggcggca
    32341 atgaacaata tggcggagca atcattatga gtgctttagc tgccgtacac agcggagcgg
    32401 gtctcacgac tgtagtaact gatgaaaaaa atcggagtgc tcttcacagt catctgccag
    32461 aagcaatgat tgtagtttgg acggatactg accagtttct ttccgtaata aagcaagcag
    32521 atgtactctt gattggtccc ggattgggta cttcttctaa gagtttggaa cgtttcaaac
    32581 aagtactaga aaaccaatca aaagaacaat ggctggtgat tgatggctct gctttaagtt
    32641 tacttgctgc taatcatctt ttgctaccct atcccgaaca aactgtactt acacctcatc
    32701 aaatggagtg gcaacggttg agttctctac cgatacaaga acaacgggat aaagaaaaca
    32761 aaaagaagca acaggaaatc ggtgctgtgc tcgttttgaa aagtcatgag acaaaaattt
    32821 atggtcacca gcacttctac accaatcctt taggttctgc tgctatggca acgggcggca
    32881 tgggtgacac tttagctggg atgatcacag gatttttagc ccagtttcct caaaatgacc
    32941 aaacgattgc agcagctatt tatcttcata gtttcattgg agaccagctg gcaaaagaac
    33001 aatatattgt tttgccgaca caaatcagcg aagcacttcc gaaatggatg aattattttt
    33061 gtaaaaaatc aatggaaaac gaggcatgag tcatggagaa atttacaggt tatgtgcgag
    33121 aaccttttta ttatgaaaca gatcaaatgg ggattattca tcacagcaat tatatccgct
    33181 ggtttgaaga ggctcgggta gccttattgg tacatctagg gtttccctat aaaaaaatcg
    33241 aagaatctgg gatcatcatc cccgttctgg aagtaaattg cagctataaa gaaatgatcc
    33301 attacggaga aaaagtgagc atccatccaa ctatccaaaa gtatacgggg acacggttgg
    33361 attttagcta cgaaattgtg ggaactgaag acaaaaaatt aaaaactact ggcagtagta
    33421 aacactgttt tttactttct gattcgcaac gattagtgaa gctgagcaaa gtcaatccgg
    33481 agttagatca gttattccaa gcgtacgctt ctttggatga tgtaaattct taataaagac
    33541 cgtctaagca tttttcttgt attgtgtcga gtagttcgta taatgaaaag aaaaaagcag
    33601 gtgatcccca tgtatgtcgt taaagtaatg cacggttaca tcgataaaac tggctgtcgc
    33661 actcgggaaa aaaacttaga caatctgctc attttcaaag acaaaaaaga atccgaagct
    33721 tttgctaaaa gaatcggcgg tcgtgtcaaa ccgatccaag aggtacgacc agattgattc
    33781 aatatttttc ttttcgaggc tctaataggc atgcctatca gggctttttt tgcagactgt
    33841 gtttttatgc ccacacgatt tgtaagcgat ttatgttata atagattata tctgcataga
    33901 aaggaaggtt aaatggttat caataatttg caacagcaag cttatgaatc tatacgtaaa
    33961 caaatcattt attccgagtt agcacctgga agtaaaatct ccgataaagt gttagaagaa
    34021 caactagcta tcaggcgtac accgattcga gaagcactga tccaactgcg caatcaagag
    34081 cttgtaaaaa ccattccaca gtccggcact tatgtttcaa aaatcgatat ccgatctgct
    34141 tcattagccc gttatacgcg ggaaaagttg gaaaaaccga tcctgcaaga atgcagtgcg
    34201 aaaatgacca agcaagatca agctaaacta gaaaaaatac tagaacaaac cgatcaggca
    34261 gtaatcgctc aaaataaaaa agcttttttt gatttggata ctgattttca ccgaacttgt
    34321 tatgaaattg ctggaaaacg agaaatttgg gattggctag aaagttatag tacgcacttg
    34381 aaccgtttca gatggttacg tttgacgatt tcagaattag actggggtcg agtattagat
    34441 gaacatcaga caatgcttca atcaatgatt gatcataact ttgatgaagt cggatttctt
    34501 tgcacgatgc atttacatat gattatcgag gagcaagaat acgtgattca taattacccc
    34561 gattattttg aagatattca ggacagaccc atcaaataag aggttttgga atcagcgttt
    34621 tgacctgagt attagagaaa gaaacggcaa aatagttcct catattttgc cgtttttttt
    34681 gagctaatac cgagggtcag ctgattgcac accgtttatt gagaggttgt gaaaaagctg
    34741 ccctgcatca agtaataaga acagccaagt aaaaatagct tcttatgttt ttaacttggc
    34801 tggacttatt accgcagatg caagcttttg aacaccgttt tccaagaggt cgtgagcctg
    34861 ccgttcatct ccgagtcaca aggaacaatt tcaaaaaaca gcttctcatg tttgttgaat
    34921 attttgactt gtgaccgagg agttgacagg agaacaccgt ttattcggaa aaaaaggact
    34981 gtgacacgac ttttgtcaca gtctctttat ttgtaatcta aatcatactc cgtacatctt
    35041 ttttctattt tttttatttc ttctaagatc ccttggctac gaatttcatt atatggccaa
    35101 agtaagactg cctctaatac ataaggcgaa aaatcgcttt gattcatctc ttttgaaaaa
    35161 ctcatcgctg ctatctcgct caatgcacga atgtttgatt gatgtgtata aaaaggcaac
    35221 cgccacagca caattttttc gctttgcgta taaattccct taacagaagc ttcgatgtgg
    35281 tgaaatagtt catgtccaat aatcaaagct tgcgctgttt ctttagagat cgaagctagt
    35341 cctgaacatt ttaatttttc taacggctcc gtcatcaatc gaatcccttc atcttctgca
    35401 taagttgcaa aagtgattcg ttcgtttatt tgctctgatt cacaggaaac gattggaaca
    35461 gccatttgtt cacaaatttc tcctacatcg gcaaaagggt actttttttt gatgctacat
    35521 gccgtttcga taccacaatc gattgattgt tgtataagaa ttttctgctt ggacaaagga
    35581 atctttttcc aaaataaatc tcgccgaaac tggtagaatc cccaaatttc ttcggggatc
    35641 tgttgtaatt cgtttagtaa ctcttctaac ttatgtttgt ttgatatttc cggcaacgac
    35701 gatcacttct tcctccggtt ccatagtaga tacttccaga tccatcaaaa gctgtagttg
    35761 ttccagatcc gtcgctgtaa aatcagagac tcttgctcct aagcagagat aaaattctgg
    35821 tcgggcaatc aattcagttt gataaactgc catatcttcc caaagttgtt cgacagctgt
    35881 catataattg gctgcagttg ttttcataca tgaagcatgt ccacgttgga ccttgataaa
    35941 tcctttttga tcgatgatcc gaagacttcc agcatttttt gaacgattct gttcgccgca
    36001 tacataaaag aaaggtgtat tttccaataa gcaaacttca tttttattta atcgcatatc
    36061 ctctgcagca agctctagtg cttcttctgt tgtgatctcc ttggttagat cggttgcttt
    36121 cacttccgta gaaccagtag cgatagccgt tacttttgaa gtctgtggat cgatttctac
    36181 gtggacttcg atcgattctg gtgtagcgcc tgattcaatc gctttattca tcgcttcatt
    36241 tttcaacgat cgtatatctt ctttacttgg cgaagggatg atacgttcta caacatctcg
    36301 gaccattgca agtgctacac caatagaaga aatcacttcg gcgttttcag gaatcgaata
    36361 tttcacaccc attttattac taaagtaagt aatcaatgag gcagcgccgc cccctacacc
    36421 tacaagagaa atctgatctt tctccaagtg atacttgtct gccaattcta aaatcaccgg
    36481 ttcaatctta gcataggatt tttccatgat ctgttctgcg atgtcttcaa ccgtcgtatg
    36541 acaataatca gctaatgctt ggatggcttt tcgtgcagat gggacatttc cataagaaaa
    36601 atgttcttct tgcactaatc caaggacatt ggctgcacat gtatttgtaa tagtcacttc
    36661 ttctccgtct tccatcacta ccttgacgta atcagcaggg tctccttctt taggagagaa
    36721 aaattcaacc ttaggacctt tgattttttc tgtctccgta aatactgcat aatctagacc
    36781 ggcaatatgt gcagaacgtg gaccgacatc gatgattcct gactgattag cccgtaccat
    36841 cgaaccgcct gccacaccta gaacccgaac atctaatgaa gagatatatg ttgggtggcc
    36901 acctacaatt gaataatcga ttgctggacg tccattttta atgaccccga tattggttgt
    36961 tgttccgcct acttcaaaat aaacaccatt agacgctctc aagtacatca aagagcccat
    37021 gactgatgct gctggaccgg aaagcatggt taaaacaggt cgttttttca tttcattgat
    37081 ttccattact ccgccgtctc cgcgcataat catcaaagaa acattgaccc ctgcttctcg
    37141 tacggaatct tctgtagagg ttgccgtatc tagcattttt ggtaaaatag atgcattgat
    37201 tgctgctgtc cgagttcttc ttgtcaatcc atataatttt gtgatgtcag aagccatcgt
    37261 tgtagggatc gattttaccg atgctgcttc ataaacaact cgttctggtt ccccattatc
    37321 tactccaaat gccatagaag aaaccaagac ctccgctctt tctctttcta aagaagaaat
    37381 cgtttctgaa acacggtcta catttaagtg tttgacaggt aagaatgcat tgacaatctc
    37441 tatttttttc ttattcccta aatcgatatc gttcaatcta gtttgtcgtt ttgctaaaaa
    37501 accttctaat ccaccttttg ccatcccgat tacgccgact tttgctacgt ctccttcaat
    37561 cagtgcatta gtcgcttgtg tggtagaatg tgcaacaaac acaacatctt ctggactgat
    37621 attattttcc agcaaacaat tttgaaaaga ctgaacgaca cctgcggcta ctccacggac
    37681 atcatcgtgg gttgttttca ctgaagattt tccgatgatt tcgtgcgtcg cattatcgat
    37741 tgcgactgct ttcgtatgtg taccgccaac atcaattccc attcgcacaa aacgtttcat
    37801 tttcttttcc tccattcatc taatcgtttt tatcgacgtt ttgtttacta ggcgttcccg
    37861 tacaataaga acgtgatcag gcataagatc gctccgatga tccaaccagt tgggatggat
    37921 ttcttcatat attctcgaga agttactttt gtataaccta atccccaagc aacccaagat
    37981 tgtgtaatat ctaaatgctg aggagcaatc gttgtgattg caaataacgg gaacaaaaag
    38041 gctggcgaca tcgttggatt aacagctaat acgacagcta aaatagctga gcctgaaccg
    38101 actagattca ttggaccgcg gaaaaatcct agtggtgtca atatagcaaa tacgattgcc
    38161 aagactaatg gtgattgcgg tatccaatct ccaaaaatag ctgaaaaata aggagaagca
    38221 tacgcagctg aattattgaa catcgctaag gtcatcaaga aaccaaccat tggcgcaaca
    38281 tctactgcgc catctgtaaa taatttcgca aactgacgac agatttcttt ataacctccg
    38341 cgcaattttc cagtaaacag caatgcccag atacctgcta gaataaaacc taagatgatt
    38401 ggaagatcta aaagaacgac ccctaaaact ggtaataata cggctaacca tgagatcatt
    38461 ggtgcgtcgc caccttcagc tggtacgttt gctgccattg catagcgttt ctttttgttc
    38521 attgatatat tagcgaccgt tagtaccaca actaagctta caatcatccc aatcatacca
    38581 atttggaaat agtcattata agtatagctt tcagccgctg gattgaaacc agcataaatc
    38641 gtttgatatt gtttgaaatt gacgatatta gcaaagattc cagccataat cgagcccata
    38701 aatgagaaca gtgcaaccgg tactgaaata cctaatgaca tcaagattgg caacacaata
    38761 actgcaatag aaatcactgg tccgattcct gtcatcgaca tgaatataac agcggttaca
    38821 atacatagta agctcatcgt gattcttggc ttgtcccctc caagttcaac tacctttcga
    38881 atcagggtag ctgcgattcc gctatctact agaacacgcc cgaaaaaggc accaaagaag
    38941 acatttacca agatcgattt ggcgtactca gctggccccg tcgtatagat atgcgtcaac
    39001 acatcaatta ctccttgccc ttccatcgcc gggtttggtg aaaacgtgtt tccgactagc
    39061 gctaatccca tccatacagt agccatgatg gcaaagccga ccatcaagtt gtagcctttt
    39121 acacagtacc agaccatgaa aaagaatgag ataactaaca aaataccaat aataatatca
    39181 ctcatgttat tcctccgtcc attttgtttt tctgataagc aaactaatat atcagtcaaa
    39241 ctcattctaa cttatgtagt tagcgctttc attaaagggt gaccataaat gttcagtttt
    39301 caattaaaaa accttaatta tgctatttta gacttatacg ttattgtcct gtttcatttg
    39361 atggaggatt gtaaataaaa aaagaggaaa gaacataact tttcgttatc ttctttcctc
    39421 tttataattg tttagcttat ttagccaaag cttcttttgc ttggtcagcc aatgcagtaa
    39481 atgctgctgc atcgttaaca gccaagtcag ctaacatttt acggttgatg tcgatttcag
    39541 caactttcaa accgtgcatt aattttgagt agctcaagcc attcatacga gctgctgcgt
    39601 tgatacgagc aatccacaat ttacggaagt cgcgtttctt ttgacgacga tcgcgatatg
    39661 cgtagttgta tgaattcatt acttgttctt ttgctgtttt aaataaagtg tgttttgaac
    39721 cgtagtaacc ttttgctaat tttagcactc ttttacgacg tttacgagtt actgttccac
    39781 ctttaacacg tgccatgtat aattcctcct aataaaaata aatcttcaga ttctgttatt
    39841 tgtacttctt atttcatttt tgctagttgt tggcgaatac gtttgaagtc gccactagaa
    39901 accatgcttg ctttacgcaa ttgacggcgt tgtttttttg ttttgccgtg gaaacggtga
    39961 cttgtaaacg cgcggaagcg tttcaatccg cctttaccag tacgtttgaa acgttttgct
    40021 gatccgcggt gtgttttttg ttttggcatg actatatttc ctcctcaaaa tctttcgtta
    40081 tccgactatc ggctgtccaa cagccagtta gtcattcaaa ttacttactg tcgtttttcg
    40141 gtgccagcgt caagaacatg ctgcgtccgt ccattttcgc tttttgttcc actgttgcaa
    40201 tatccgcagt ttcttcagct aagcgattca agactttttg accaatttct ttatgggtaa
    40261 tggcacggcc tttgaaacgg atagaagctt tcactttatc gcccttttcc aagaacttac
    40321 gtgcattacg aagttttgta ttgaagtcat tcaagtcgat cgtaggactt aagcgaactt
    40381 cttttacatt gatcactttt tgttttttac gcgcttcgcg gtctttcttt tgttgttcga
    40441 aacggtattt accgtagtcc atgattcgcg caactggtgg tttcgcacca ggggcaacta
    40501 acacaagatc caagtttgct tgttctgcaa tattcaatgc ttctgctttt gtcttaactc
    40561 ctaattgctc accatctgaa ccgatcaatc gtaactctcg tgcacgaatg ccgtcgttaa
    40621 ccatcatatc ctttgctatg gtcattcacc tccaaatatt atgagagaaa gaaaagctta
    40681 ccgaataaac ggcaataaaa aaacgagttc tcttacagaa cccgcgcgta cttcacctaa
    40741 tagaaccagt gaaaactatc tagcccagtg acattatcaa ctaaggcgag aagcgggtgc
    40801 ttctgctttt atttctcaac catcatagta tagcaaaaac taatcttttt gtcaatgttt
    40861 ttcatacagt ttgtcttcct tttctgaaaa tgattagatt agcagacttt ctagcagtga
    40921 ttcccgcttc tttgctatac taaagaaaaa cgaggtgaac ccacatgaaa aaaatcgaac
    40981 ctattccacc ttctcttcct cttcttacaa agggagagtt ttatttagaa gatgaggaga
    41041 tcttctctgg tcttctgatc aacgggggtg accaaagcta tctcgatgcc aaaaatatgg
    41101 tattaagaga gagtcatatc caaaaactta cgatgcaaaa aacagtttta cgtcattttg
    41161 aatgcagtaa tgtcattttt gaaaaatgtg atttttccaa tctggagtgg attggtggca
    41221 gtttccacca agtcgtgttt catcaatgta aattaacagg tacgaatttt gccgaaagtt
    41281 atcttcgaga ctgtactttc actgattgta ttgcttcgat ggcttccttt agtagtacaa
    41341 acttgaaagc tgtctccttc gatcattgtc aattagaaga cagcgaattt tatgaggtca
    41401 cttggaaaaa cctttttttg tctgacaatc agttgaccgg ttcgaactgg tttcgaactt
    41461 ctctgaaggg attagacttt acgactaatt tcttcacagc aatcgcttta tctcaagatt
    41521 ctttggcggg actaatcgtc aaccaagagc aagcacttgt gatcgcccaa gcccttggtt
    41581 tgcagatcaa agactagatc aacgaatccg ccgccacagt gcaataagca tccaaataga
    41641 taagaccaaa ctgatgatca aggcgattcc ccatccgaaa acggaatggg taaagggtaa
    41701 ctggagattc attccgaaga atcctgtcac aatagttgga acggtcaaga tcagcgacca
    41761 gaccgtcaaa aatttcatcg tgtcattcaa gttgttattc agcaaattat tataagtgcc
    41821 agagagctgg tctaaaatct gcgaagctaa gttcgtcatt tcaactgctt gtcgtgcttc
    41881 gatcagcgca tcgtccaact cttctttttc cttttctgac aacttgcgat aaatagccaa
    41941 tgccttgatt tgttctaaaa gtactacatt ttgttttgtt cctgtaacta agtaaaccaa
    42001 accgatctct aaatctgaaa gttgcagcag attcttgttc gttgtttttt ctcttaattt
    42061 cagattaagc cgctgacgtt cactattcac ttcctcgatt aaaggaaaaa aggaatcaga
    42121 aatcaaaaac aatgtacgaa acaataaact gtatacactc atgtctggca ttcgttccaa
    42181 aagcgatcga attagtcgaa tcacgtactc ggtattcggc gtggtaaatg taaacaaacc
    42241 gtcttctttt aaaataaaag cgataggact agtttcataa tgattatcga ctttttgctg
    42301 gtgcggaaca tttgatacaa gcagtaacgc ttcatcaaaa gaatcgtact ctacgcgcgc
    42361 ccgttcattt ttatctagag aataagtcag catctcgtca ctaatgccat attcttcttt
    42421 taagttttta acagctagca catcgttttg ctgtacattg atccaaaaat tacggtcttg
    42481 attaaaatag tgtttttccc gcatactcta tcctcctgac atttgtttcc agtataaaga
    42541 aaaaagtcgc tcaaataaag gtttaccctt tatttgagca actgtaaatc aaatggctac
    42601 tttggtttgg cgaatctctg ctaatttttc ttcgatcatt ttcaagactt cttcttttgc
    42661 ttgaggatct tccacaaaat tatagcggtc tccatcgatt tggatttttg gactttcttt
    42721 gtattcttca taccattgat cgtaacgtct gttcaattct ttgtaataat cgtatagact
    42781 tggatcaaag gacaattgtt catacgaacg accacgtttt tcgatacgct ccaacatcgt
    42841 atcaaaagat acacgaatat gaactaataa atccggatgt tttttatggg cagcataggg
    42901 aagttcttcc atcatgtttt ccagcagttc atcatacaca cgaacttctg tttctgttgc
    42961 tcttcctaga tctgcattta gatggaaaag cagtgaatct tcataaatcg aacggtccaa
    43021 tacattattg tcatctttca ttgcttgttt tatgcttgca aaacgtttat tcaaaaaata
    43081 aatttgcagc aaaaaagcat actgttcagg gtttgcataa aataagggca agacttcatt
    43141 atcatcaatc gattcataaa atgcctgact atcaaagtgt tcagccatca tttctgttaa
    43201 acttgatttt ccggcaccaa ttgtacctgc taaaacgatc acgctcattt acctccagct
    43261 cacttcatca ctaaatattg tgtttaactc caaccttttt aaatataccc ccaatatcta
    43321 gtagtgtcaa ttgtattttt ttctagtcaa agaagaaaaa aagtcaaaaa attcttaaga
    43381 aaaaagtgtt ttttctttag aaaacgtatt cttagtttta cttctgagat ggttttagta
    43441 aaataaagga ggaatgtttt ttgagaccct tagtggacaa aaaaattcgt gaagcagcgc
    43501 aactcgtttt gggcgcttta ttttttgctt aaaaatcact tttacgtgag aaggagttag
    43561 attatggcaa actatggcca aggagaatca agcggaaaga tcatattgat gggcgagcac
    43621 gcggttgttt atggagaacc agcgattgcc tttcctttct atgcaacaaa agtcaccgca
    43681 ttccttgaag agctggatgc aatggacgat caactggttt cttcctacta ttcaggaaat
    43741 ttagccgaag ctcctcatgc attaaaaaat atcaaaaaat tattcattca cttaaaaaaa
    43801 cagcatgaca tccaaaaaaa cttgcaactg accattgaaa gcacgattcc tgctgaacgt
    43861 ggaatgggat caagcgctgc agtcgccaca gcagtcactc gtgcttttta tgattactta
    43921 gcatttcctt tgtctcgtga aatactatta gaaaatgtcc agctttcgga aaaaatcgcc
    43981 cacggtaatc ctagtggaat cgatgcagcc gctactagca gcttgcagcc gatttatttt
    44041 acaaaagggc atcctttcga ctacttttct ttgaacatcg atgctttttt gattgtcgct
    44101 gatacaggaa tcaaaggaca aacaagagaa gccgtcaaag atgtcgctca cctctttgaa
    44161 acccagcctc atgaaactgg acaaatgatc caaaaattag gatacttgac gaagcaagca
    44221 aaacaagcaa tcattgaaaa ttcaccagaa acgttggcac agacaatgga tgaatcacaa
    44281 tcacttctgg aaaagctgac aatcagcaat gattttctta atctattgat ccaaacagca
    44341 aaagataccg gggcattggg tgccaaatta actggcggtg gacgcggtgg ctgtatgatt
    44401 gcattagcac aaacaaaaac aaaagcccaa gaaatcagtc aagcacttga agatgcgggt
    44461 gctgctgaaa cttggataca aggattagga gtacatacct atgtttaaag gcaaagcacg
    44521 cgcatatacc aacattgctc taatcaaata ttggggtaag aaaaatgaag aactcatcct
    44581 tccaatgaac aacagccttt cgttaacatt ggatgcattt tatacagaga cagaagtcat
    44641 cttttctgat agctatatgg tagatgaatt ttatctagat ggcaccttgc aagacgaaaa
    44701 agcaacaaaa aaagtcagtc agtttcttga cctttttcgt aaagaagccg gcctctcact
    44761 aaaagcttca gtaatcagtc agaatttcgt tcctaccgca gctggattag cctcctctgc
    44821 cagcgggcta gctgctttag caggcgcttg caatactgct cttaagcttg gattagatga
    44881 tctctctctt tcaagatttg ctcgacgcgg gtctggttca gcttgccgaa gtattttcgg
    44941 tggtttcgtc gaatgggaaa aaggccatga cgacttaagt tcttacgcta agccagtccc
    45001 ttccgattct ttcgaagacg atttagcaat ggttttcgtt ttgatcaacg accagaaaaa
    45061 agaagtgtcc agcagaaatg ggatgcgtcg gacagtcgaa acatccagtt tttatcaagg
    45121 ctggttagat tccgttgaag gggatctata tcaattgaaa caagcaatca aaacaaaaga
    45181 tttccaactt ctcggagaaa cgatggaaag aaacggacta aaaatgcatg gaacgacttt
    45241 agctgcccag cctccattca cttactggtc tcctgacagc ttaaaagcga tggatgcggt
    45301 gcgacaattg agaaagcaag gcattccatg ctactttacg atggatgctg gccctaatgt
    45361 caaagtttta gtggaaaaca gccatttatc tgaggtacaa gaaacattta ccaaattatt
    45421 tagcaaagaa caagtaatta ctgcacatgc tggtccagga attgcgatta ttgaataaag
    45481 ggagattttt atgattgaag tatctgcacc aggcaaactc tatattgccg gagaatatgc
    45541 agttgttgaa acaggccatc cagcagttat cgctgcagtc gatcaattcg tgacagtcac
    45601 tgtagaatct gcacgaaaag tcggaagtat ccaatctgca caatatagtg ggatgcctgt
    45661 acgttggaca agacgcaacg gagaattagt tttagatatt cgggaaaatc cttttcatta
    45721 tatccttgct gctattcgct tgactgaaaa gtatgcacaa gaaaaaaaca ttcttttatc
    45781 cttttatgat ctgaaagtaa caagtgagtt agacagttca aatggccgga aatatggatt
    45841 gggttctagc ggtgccgtga ccgttgcaac ggtcaaagcc ttgaatgttt tttacgcgtt
    45901 gaatttatct cagttggaga ttttcaagat tgcagcacta gccaatttag cagttcaaga
    45961 taatggttcc tgcggcgaca tcgctgctag ctgttatggt ggctggatcg ctttctcaac
    46021 cttcgaccat ccttggctcc aagaacaaga aactcagcat tctatcagtg agttacttgc
    46081 cctggattgg ccaggtctat ccattgagcc attgattgct cctgaagatt tacgtttatt
    46141 gattggttgg acgggtagcc ctgcctctac ttctgatttg gtcgatcaag ttcaccgttc
    46201 gagagaagat aaaatggtgg cttatcagct tttcttaaaa aatagtacag aatgtgtcaa
    46261 cgaaatgatc aaagggttta aagaaaataa tgtaacgttg atccaacaga tgattcgaaa
    46321 aaaccgacaa ttactgcatg atttatctgc aatcactggg gtcgtcatcg aaacgcctgc
    46381 tttgaacaaa ttgtgtaatt tagctgaaca gtatgaagga gccgcaaaat cttctggtgc
    46441 aggtgggggc gattgcggaa tcgtaattgt tgaccagaaa tctggcattc ttcctttaat
    46501 gagtgcatgg gaaaaagcag aaatcactcc actgccgtta catgtctata gcgatcaaag
    46561 aaaggaaaac cgatgaatcg aaaagacgaa catgtttcac tagctaaagc ctttcatgat
    46621 aaacaaaaaa acgaatttga cttcgtacgg atcatccata accctttgcc gcaaatagca
    46681 gtttctgatg tcgatctaag tacacaggca gttgggttta cactaagcag cccattttat
    46741 atcaatgcga tgactggcgg aagcgaaaaa acaaaaaaaa tcaaccaaga tcttgcaatc
    46801 gtcgcaagag aagcagattt gatgattgcc actggttcag tcagcgctgc cttgaaagtc
    46861 ccttctttag ccgatactta tacgattatg cgacaagaat atcctcacgg gaaaatcatt
    46921 gccaacatcg gtgcagggac ttctgtcgaa agagccaaag aagctatccg attgtttcac
    46981 gcagatgcct tacagattca tttgaatgca cctcaagagt tggttatgcc tgagggagac
    47041 cgtgatttta ccaattggaa agtacttatt caagaaactc aaacagccat tgacgtcccc
    47101 ctcatcgtaa aagaagtcgg ttttggcatg acaagagaaa cgctcaatga tctagctgct
    47161 ttaggagttc atacagttga tatcagcggt cgaagcggaa caagcttcac ccagatcgaa
    47221 aacgcccgtc gctccaagcg ggaactgagt tatttagctg actgggggca atcgacagtc
    47281 tcttcattgc ttgaagcaaa tgaagcagac acttctatgg aaatcctagc ctccggaggt
    47341 atccgcaatg cctatgacat atttaaagcc ctttgtctcg gcgcaaatgc cgtgggtact
    47401 tcaggaaccg tactgacgca tctgatgaat cacggcgtag aagaaactat tatactgatg
    47461 aagcaatggc aagaagagct tcgtctgctt tatactatgg tcggcgcaac gaataccgcg
    47521 gccctacacc aacaatctct gatcttttcc ggaccagtga aggattggtg cgaagcaaga
    47581 gggatcgact tagtgaagta tggaagacgc acagaaaaga gcgtgggaca aaagtaaaga
    47641 acacttttgt ctcacgctct aaacacgtac aaacggcgga agtaaaagca actccttcgg
    47701 aaataagctg aaattcacaa aaatttgaaa agcaattttc gtgaattcct tcttatttct
    47761 tggagttaaa tgcttctgtc ccagcctctt ttttcgaata aatgatgttt aatcagctgt
    47821 ccttcggtat tcgttcaatc cgctgatttc acaacctctc cactaaacag tgtactaaag
    47881 ctcttttaag gttgttttcc gatataggca agtatgcctc cgtccacata caagacatgg
    47941 ccgttaacga aatcagatgc gctagaagcc aagaagacag atggtccagc caaatcgatt
    48001 ggctcgcccc agcgtgctgc tggcgtacgt ccaacgataa attgatcgaa tggatgacgt
    48061 tcaccgtttt cttgtgtttc gcgtaatgga gcagtttgcg gtgtagcaat ataacctggt
    48121 ccaattccat tacattggat attgtattga ccgtattcag aagcgatgtt tttcgtcagc
    48181 atttttaatc cgccttttgc cgcagcataa gcactgaccg tttcgcgtcc taattcactc
    48241 atcatggagc agatattgat gattttaccg ccgccttttt cgatcatatc cggaataact
    48301 gcttttgaaa cgatgaatgg ggcatttaaa tccacgtcga ttacttggcg gaagtctgcc
    48361 gcatccattt cgatcatcgg tgtccgtttg atgatccctg cattgttgac tagaatatca
    48421 actgaaccta catcttcttt gatctgttga accatcgctt gaaccgcttc ttcatcggtc
    48481 acatcgcaga cataaccttt tgcatcgatg cctgcttctt tataatgcgc tataccttca
    48541 tcaactgatt cttgattcaa gttattaaat acgatttttg ctcctgctgt agctaaagag
    48601 cgtgcaatct caaatccaat accatagaca gcaccggtga ctaatgctac ttttccatct
    48661 aaacgaaaca tatccatact aaattccatc attttctctc ccttatgctt attttaattg
    48721 atccatttgc accatatcca tgtctgtata ggtgatattt tctccgcaca ttgcccagat
    48781 gaatgagtaa ttgcttgtac ctacacctga atggatcgac cagcttggcg aaataatggc
    48841 ttgttcattg tctacaacta aatgttttgt ttcatctggt ttgcccatca tgtggaacac
    48901 acgtgtatct tcattggcat aatcaaagta gacataagct tccatacgac gttcatgtgt
    48961 atgacaaggc atcgtattcc aagcgcttcc tggttctaaa atcgtataac ccatttgtaa
    49021 ttgacagctt tcgcatacat ttggatggat gtattggtag attttacgtt cattcagtgt
    49081 caggccttca ccagtctcca ttggttttac ttggtcgata ctgattttta catttggata
    49141 tttatgatga gctggaacag aactaatata gaatttagct gggttttcag catcttgaga
    49201 agaaaataca acatgtcgtg tttcttttcc gatatagtat ccgtcttgtt ttttcatttc
    49261 ttctttttgc ccatcgatct cgatgaatcc tggtccaccg atattgatca cacctagttc
    49321 acgacgctct aagaagtaat caacacctag ttctttgtct aatgtgattt ctaattcttt
    49381 ttcagtaggt gtcactccac caaagatcat tcgatcgttg tgcgtataag tcaaactgat
    49441 tttccctggt tcaaatacct tttctactaa aaattctttt ctcaattgtt ccgttgaata
    49501 atgacggata tcttctggac tgtgtgtata acgtgtttcc atttcttgca ttttcttcta
    49561 ctccttctat cgaatgattt ttccagagcc gcatgctaaa aattgtgcga cttcttcttg
    49621 actaaattgg ttgcagtccc catggatcgt atgcttcaag gcagaagctg ccgtagcaaa
    49681 atcaatgatt tcttacggag acatattgga taaaatccca tggagcacgc cacctgcaaa
    49741 agcatctccg ccacctactc gatccacgat tggggtgatt ctatggactt gggattccac
    49801 atattcatgg ttcatccaca atgtcccctt caattcattt tcacttgctg aatgaacggt
    49861 acgcttggtc gaataaaaca cctgaatatt cgggaacttt tcctgcattt tttgataata
    49921 ataaattaga ggttgttctt ctgcttctgg cgctttctcg atacctaaca aataaagagc
    49981 atccattttt ccagctgagc agatatctac taaaggtaaa agctgatgga ttacttcgct
    50041 ggcttcttgc tgtgtccata attttccacg atagttgata tcgaagctga tcaggcagcc
    50101 agcttttttt gcagcttcta tcacctgttt cgtcattttt tgccattttt tagacaaagc
    50161 tggggtgatt cctgaaacat gaaatatatc tattccttgg aataactcct ctagcgacca
    50221 ttccaaatga tccatttcag caaaacttga tccggcacga tcatagatca ctgaggcggc
    50281 gcgttcgcca ctaccagttt ccatataata agtacctagt cttgatccac cagataatac
    50341 ctgtttcgta tctactccat aacattgcaa gtgctttttg actgcttttc ccaatgcgtt
    50401 atcaggtact tttgtagcaa aaatcgcttc atgtccgtaa ttagctaaag aaattgctac
    50461 gtttgcttcc cctccgccat aatgggcagc gaatcgttca cttgaatcga tacgggttcc
    50521 ctgctctgtg gacaatcgca acatgatttc tccgagtgtc actacttttc ccataagcag
    50581 ctacctcttg atttctttca tttttgctgc atactgttgc gcgacttccg ttactttgtc
    50641 aaaatctcct gtagctgcgg gtgctaaaag gttcccacct actccgactg ttacgacacc
    50701 agcatcgaac cactctttca tattgtccag actcacaccg ccagttggca tgatattgac
    50761 ttgaggcatc ggcgccttga aggcagaaat gatacttggt ccataagcgc ttccaggaaa
    50821 tagtttgacg atatctaccc cgcttttcag cgctgttttg atctcggtaa tagtcataca
    50881 tcctggtaag taaggaattt gatacagatt gcacatctct gccgtttctt gatcaaagga
    50941 agggctaaca atatactctg cgccagctaa gatagccaag cgagctgtga cagcgtctaa
    51001 tactgtgcct gcgccaatca ctatttctgg ttggtctttg tagaatgaca aaagttcttt
    51061 gattacttga tctgcttgag ggactgtaaa agtcaactca ataccagtta gaccaccttt
    51121 gatgattgcg tggcaagcat tcaaagcttc ttctttgctt tttccgcgta ccaccgcaat
    51181 cactccggcg tttttcaatc gagataaaat atcaattttt ttcatcttct ccacctattt
    51241 cctatttaga aattttattt tataacttgg aaatctatac ccatattata cggcagaaaa
    51301 gaaaaattgc aaacgctttt ttcattattt ttgaattctt tgctcgattt cgctttttgt
    51361 ttgcttgagg gcactgatta tttgttcttt tgtctcatct gttaaacgat atttcggcat
    51421 acttacacta aatgcaccaa tgatttgttc gtttttcacg attgaggctc ctatacaaaa
    51481 aatatcacgt tcgatttctt catcgtcaaa tgcgatgtgg gtttctttta ccgttttgat
    51541 ttcctttttc aaccgtaatg cattggtgat cgtatattct gtgtaaggga tcaacggatg
    51601 cttttcaaga taatcagtgt actgctcttc actgaattct gctaatacgg ctttgcccat
    51661 cgcagaattg tataatggtc gggtgattcc gatttttgaa gacatgcgga tcgtttgatg
    51721 ttttggatct agtttgttca cgtacaagat ctcattgttg tccaataccc ctaagtggat
    51781 agtctcatcg atcttttgct gcagctgttc taaaaacggt aaagtcaact ctaccaaatc
    51841 gatttgttca atgtttttat tcgcgtagcg aataagcttt gcacctaaac gatagttttt
    51901 ctctgaattt ttatttacgt agccgatcat caacaaagta tctagtattt tcaagactgt
    51961 agaagaggtc attcccacac cctttgcgat cgtctgcaaa gagacatctt ggttttccgc
    52021 taaatagtct agtattttag aggctttgat caatacagtt ccataaggct ttttattttc
    52081 catataatat tctcctattt ctttataaga aaaattatag cggaaatacc atcaatttaa
    52141 aagagatatc cagatataaa ctaccatttc tctttacagg caatttaatg ttgaagatcc
    52201 gcttcgtttt tacatcaaac tttgatataa agcaatgatt tcttctactg tcgtttctct
    52261 tggatttcct ggtgtgcata catctcgcaa agcatcttct gcaattgctg gaatatctgc
    52321 ttcttttaca cctaattcag aaatggtttc tgggattcca accgctttgc tcaaccgatc
    52381 gatctcacca caagccgctt ctcttacatc ttctaaagac atttcagctg ctcctttaat
    52441 ccctagaaca agagcgatct cacggtactt ttctccagta tattctttgt tatatttcat
    52501 gacagttggc aaaagtgctg cacacgctac gccatgagga atgttgtacc atgcacttaa
    52561 cgggtgagcc atcccgtgta ccagtcctaa accgacattt gagaagccca ttcccgcaat
    52621 atactgtcct aatgccattt tttctcgccc attttgatcg ccatctacag aagcttccaa
    52681 agaatggccg atgatttcga ttgcttttaa atgcagcata tcagtcattt cccaagcgcc
    52741 ttttgtgata aacccttcaa tagcatgcgt catagcatcc atacctgttg atgcagccaa
    52801 ttttttcggc attcccatca tcatatcgct gtcgatgaac gcgacgattg ggatatcatg
    52861 aggatcaaca caaacaaatt ttcggtgatt tacttcatct gtgatgacgt agttgatcgt
    52921 tacttctgct gctgttccag aagtcgttgg tatagctaat ataggtaaac atggattttt
    52981 cgtatcagca acaccttcta aactaatgac gtctgaaaac tctggattcg tcaaaataat
    53041 cccaatggct tttgccgtat cgattggcga acctccaccg attgcgatca aacagtctgc
    53101 ctctgcctct ttaacaaaag ccactccatc ttttacattt tggatcgttg gattaggtac
    53161 gatcccgtcg tataaagcat aagcgattgc tgctttatct agaagttctg ttacttttgt
    53221 tgctataccg tgctcgatca atcctttgtc agtaatgaca gctgctttgc cgaatcctcg
    53281 ctgctgaaat tcagggacga tcgactcaat cgctcctttt ccaaaatacg atgtttcatt
    53341 taaaatcatt cgattactca ttttctattc ctcctaattt gtattatttt tcacaataaa
    53401 aacgaaagaa ccccaagcaa ccggttgccc agttgcttgt tgctacagtt caaagactgg
    53461 catcaattcc caactgactg gagaggcatc tggatttgtc tccatgatat cttccatgta
    53521 cttccaccat ttttgattga tagatgtatt tgccattttt tcccataact tttcatcttc
    53581 gatcactaag taaccaaata acaaacttgt actcggatca agatagatcg aatagtcgat
    53641 gaccccgtgt tcacgaagtg ctgtacgcat ttctggccaa agttcatcat gtcttttttt
    53701 gtattcttct tgtttttctg ggtaaacttt catgcaaaaa gcttttttca ccatgtcaga
    53761 cacctgcttt cgtatgaact tcctccaaat aacctgcttt tggagtgaca ccaaacgcat
    53821 ctgctaaatg ccacaggttt tcgtccgtga ttgtttgcaa gattggtcct tgcgctttga
    53881 cgtacgtata gacttctgct gccttttctg ctgtctcgat caaaccaaag acttcatcca
    53941 tgtcttttcc tgacccgtag attccatgtt gtggccataa aaccaatcga gtttctttca
    54001 ttttttcagc tgttgcttcc ccaatctcat ttgttcccgg aaccatccac ggaatgatgg
    54061 aaattccttc tggaaaaaca actaagcatt ccgtacacat ttgccatagc gtacgactaa
    54121 aggaacgttc gtctaattca tgcgtaaaac tcatcgctaa tagatgacta gcatggcaat
    54181 gcataacgat ccgattatct gggtctactt ctaaacgggc gatatgactc atgaaatgac
    54241 ttggcaactc gcttgtagga aggcattcgt tttccagccc ccacaaaagt tccagtgttt
    54301 tgccatcttc agcaactcga acgattccaa gattctcggc aggttcgctc gctacatttt
    54361 taaaatatct tcctgaacca gttacaataa aatagcatcc tgctaaacgg ctggcatcaa
    54421 aaatcatcga gatgcggcgg atcacttttt caggatccaa gaacggtgtg atctcttctt
    54481 cttttagtaa ataagaaata ttcccgccat ttctttcatc ccatcccaag cgataaagat
    54541 tcgacgtcgt ttcgatcatt tctttcacat aaggcgcttg taaaatatct atttttttca
    54601 atatagccac actcctattt tcttaattcc aacacgtctt tttgatattc ctggacttct
    54661 tccagccaat ccaaaccaac tggaacttga tttttgtaac agaaataatt ccatacatcg
    54721 gcatacggga aatctttcaa ttcttccgta taagccattc ttgaagtaaa gtcaaaagcc
    54781 agctccattt cttccaatcg ctcaactggt tccagcatag ctttcagcag tgctttttgt
    54841 gtatttctcg taccgatgac ccaagcagcg atacggttga tcgttgcatc aaagaaatcc
    54901 agaccgatat ttgttttttc caacaattcg ttacggacta attctttagc gatttcctgc
    54961 aattcatcat ccatcaaaac cacatggtct gaatcccaac gcactggacg ggaaacatgc
    55021 aacatgattc ctttgctgaa taaagctaat gaagataatt tattggaaat cacttctgtt
    55081 ggatgaaaat gtccagcatc tagacaaaca agtttgtcac gagtcaaccc gtaacccata
    55141 taaaattcat gggaaccgac tgtataagct tcagcgccta aaccaaatag tttgctttct
    55201 actgcatctt gtacataccg ttcatctaac tcttctgtaa aaatttcatc gagtgattcc
    55261 attaaacgtt cccgtggggt aaaacgatca atcggattat ctttcatccc atcaggtacc
    55321 caaaagttat tgatacttgt ttgcccagtt tctttcccaa agtactccgc gattttgcgg
    55381 cttcttttcc cgtgttcgat ccagtattca cgaacttctt tatctggatg agccaatgta
    55441 aagccgtctt tcatcattgg atgcgaaaag aaagtcggat tgaagtcaag accgatttct
    55501 tctttttttg cccactctac ccatttttca aaatgtttcg gctcaatttc atttagatcg
    55561 acagattctt ctgtatccaa atagatagca tgaaggttca atttgtgttt gcccggtatc
    55621 aacgaataag ccttctccag atcttgtcta agctcttcag gtgttcgtgc ggctcctgga
    55681 aaattccctg tcgccatgat tccaccggtc aattcgccat ccggtgtcaa gaaacctttg
    55741 acatcatctc cttgccagca atgcatagaa attttgatgt cctgtatctt ttctagtaca
    55801 gcatctgtat ctacaccgat agttgcatat ttttcttttg cttgttcgta atttctttca
    55861 atcgtgttca tacgattact cctttgattt tattcacgta ccattccatc gtgttttgct
    55921 tattttcatc tgggtaaaaa gtttcacatg gaaacgactg tttgattaca tttcttgctt
    55981 gttctatatt cgaaaactga cctgtactga gcatttgcat ggcaacattc ccgattgctg
    56041 tggcttcact aggtcctgct tctacaatga ttccggcata atctgctgtc aattgattca
    56101 gtagacgatt attcgatccg ccccctacga tgtgcagctt ttccaatttc gttccagtta
    56161 agtgttctac tttgatcagc tcttgtgcgt aacaaagagc aagattatca tacacacaac
    56221 gagcgatagc tcccgatgag acagggactg gctgctctgt ttcaaaacag tattgctgaa
    56281 tctcttctat catatttggg ggattcaaaa atcgagggtc attgatttcc atcacttgct
    56341 gcagcggttt ttcttttgca gcaagctctg ccaattccgc ataactgtac tcgtactctt
    56401 gcattcgggc aacttcctgg atcaaccaca tccccatgat gtttttcaaa aaacgcaccg
    56461 tgttatgggc accccattca ttcgtataat tttcagaaaa agcattgcga gaaacattgg
    56521 cgatctttgt ttcaatccct aaaagcgacc atgtcccact gctgatataa ccccaacgtt
    56581 ctccttgacc tggcgttcct aatacagctg acgccgtatc atgacttgct actgtgataa
    56641 aggtcacatc tggcaaatca tattttgaaa attgttcttt ttgcagatct cctagtatcg
    56701 ttcccggctc tgtcaatggt ggaaataatt ccttcgagac accgattttt tcaagaagtt
    56761 ctatttccca ttcttttgtc cctgcattca gcagctgcat agtagatgca ttagttttct
    56821 ccgtcaccat gttaccagta aagacatacc ctaaataatc aggtatcagt aacaatttat
    56881 gtgcttttgc caaattttct ttttcttcag caaacaattg gaacaaggta ttgaaaggct
    56941 ggatctgaat ccctgtctgc tcatacaact tatctagaga atagatttca gaaaactgtt
    57001 ctactgctcc attggtacgc ttgtcccgat aagcaatcgg atcatttatc cgatcccctt
    57061 gttcatcaat cagacagtaa tcgactgccc atgtatcgat ccccgcaaaa aggcgatcaa
    57121 tccctgtctg ctttaatttt tcaaatccaa tcaaaatctc tttgataaga taatcgatct
    57181 tccagcgatc gtgaccgtct ttgttccgaa agccgttttt gaaccggtgt acttcttcca
    57241 agatcaaaga accttttgca tccctcgtac ttttcattag tcgtccgctt gatgcaccga
    57301 tatcgatggc aacaaatgtt ggattcattt ctccacctct tttactttat tgttgattag
    57361 cactttatta ataaagcgtt atcatttatt gacaatgcta tcataataca gaatcttata
    57421 ttttgcttca cactattttg gtatataatt gcattatctt gacaagttgg gagtgtttac
    57481 atggattact gggaaaaagc caaccactca tggtcggaag actcaaaaag agtgatcttg
    57541 acgcctacac aaagaagcag gaatttattt ttttatatcc aagaaatcgg ttacttcaaa
    57601 gcaccaatcc cttactatac tgaacgagca catttacctt cttacttgat aaagtttact
    57661 ttgtctggca aaggttcgct tacatataat gataatcagc aacagctaaa agctggagac
    57721 ttatttttta ttgactgcca agaatatcag cgttatcaaa cgatcagcga agaaccatgg
    57781 gaaatggatt ggatccattt gaatggtcag aacgtccaat cattttatca ggagttcaca
    57841 aaagacggcc ataacacctt ccattcaaca ggagatccat cagaaaacaa gatccaccat
    57901 ttgatcacag aattgatcca catgcaacaa ggcacgaatg cccgtaccga tttccagtcc
    57961 tctgtagcta tccacgagtt gctgaatgaa ctgattttgc aaaagtatca gctagatttc
    58021 gaagaagagg atattcccgc ctacgtttac aaactgaagc aatacttaga cgaacatttc
    58081 aaagaaacga ttacacttga tcaattagaa caacaattct tattgaataa atatcagctg
    58141 agcaaagatt tttccaaatt tatcggttct ccaccaatcg attatttgat cagcaaaaaa
    58201 ctttcttacg ctaaagacct tcttcggtat acagaattta cggttcgaat gatttccgaa
    58261 gaaataggga ttgagaattt tgcttacttt agccgcctgt tcaaagcaaa aacaggacta
    58321 tcgccgagag actttcgtaa attcagctga ttcatataat atgtacgtca ttttttcaaa
    58381 atattttgaa atatatagat aaaaataaaa taaccgcagt ttctgcttct atgtaaacaa
    58441 cagcagctac tgcggttatt ttttagtgat cattctcatt gttcatcttc aaaatgttca
    58501 taatgaacca actgtttctg tgtatcacgt ttcaaaaatt ctttaggcgt tatgccgaac
    58561 tctctcttga atgctttcag aaaatgcgga taagaagaaa aaccactttt actatataca
    58621 gcactagagc tgcggtattc tcgcaacaag tgcttggcat agatcagctt tttcttcatt
    58681 acatactgat gaaacgtaaa tccagtgtac tttttaaatt ctcgtgtgat atggtatttg
    58741 ctgacaaaaa actgcttttc catttcctct aaagaaagcg gatgagataa attttttgag
    58801 atatattgta acatttgttc cacacgctca ttaggaacta cagcattctg ttcttgcgca
    58861 ctctcttctt tttgtacaac ttgattgaga aaaatcaagt attctaccag agcttgttca
    58921 tattttatgt cttcaccgta aattttttta tcaggaggat ggtcgataaa tgataatttt
    58981 tgcttcaata cttcctgttc tatttgcaat acacgactcc tcttctctcc cattggttga
    59041 aaacaagccg atagattaga ccatggcgtg gaacgacttt ctagaaaaga aggggtgaca
    59101 aagatataga cacgctcgaa tacatctgaa gattggcgga cgatacgatg caaatcattg
    59161 gaatggatga gcagcacact tcctgctttg atcgtaaatt gtcgtccgtt caaataaaaa
    59221 gtcgcttctc cctttaatgt agcatggatc tcatagaaat catgatgatg atagatcgtt
    59281 ttattttggc tgacgacatc tttgacatga tagatttcat actgatcaga aaccatcgta
    59341 ttgacttgtt caccgctgat tttcttttgg agcatgtgcc gcacctcctt ttgttcactt
    59401 atgcacaatt ttattatacc acgttttttg taatcaaaaa gagctgcttt tatatactct
    59461 tataaaaaag aagagacgtg gcatcttcta gccccgcccc ctccattcta aatattagtt
    59521 aaccaacgtc ttctttacat tattcaagaa gacttcttcc ataacgaatc ccatcatctt
    59581 ttgtttcttc aagaaccaca tacaggtacg gacgattctt catgatgatt tctttgattt
    59641 ttgtgtactc gatcacaccg tctcccaaat ttaccggcac gattttttcc tgttcgacac
    59701 gaaaatcttt caaatgaacc gcacatatct tttcaccaaa acgttcaaaa gcttcttcta
    59761 ccaattggac ttgttcttga taatcactac ttgtgatcaa attggtcgga tctaaaatga
    59821 ttcctagata gtccgaatct actgcttgga tcagctgctc tacccttgct aaagaataca
    59881 ccggatgatt caaacctggt tctatcccca ccatcatttg gtatttttct ccagctttta
    59941 ccaagcgacg gataacggac acagcttcag caaatgcttc atctgtgaaa ttttcttccg
    60001 tatattggat ttccggcaaa acacatccag tttccgaagc aaccatcgac gcaccaaagg
    60061 aagaagcata gcgaagatac tgctcgaact ttgtcagcaa ctcttgtcgt atcgttaaat
    60121 ctggatgaat catattgata tagcaactta aaattcctac ggtcacttct tgcttttcta
    60181 aaactcgttt gacataattt cccatcccag aattgatctc atttgcgcca gaaggtattt
    60241 caggaaacga aagacctaag gcgagctgca gtgtatgaat cccttgttct tttgtttttt
    60301 ctgcaagatc ttctatcgtt tggacttcgg aaagatcatg tcctctaatt gtaatgttca
    60361 acataactat tctcctaact cagcatattg ggtagccaca tgacgatttg cgggaagtaa
    60421 gtaatcaaaa acaagacgac taagataacc ccataaaaag gaagaagcgg tttcagcatt
    60481 gcctctgctt ttacgcctcc tacacttgca ccgacatata gtcctgtacc tactggcggc
    60541 gtgatggagc ctacacacag attcatgatt gaaaacaacc cgtaatgtac aggatcaacc
    60601 cctacacttg tgacgattgg taaaaatatt ggtgtaaaaa tcaagatagc aggcgccaca
    60661 tccataaaca tacccaccag taataacaca agattgacta ataacagaat aataaattta
    60721 ttatccgtta cgcccataat caacgaacta attgcctgag gaataccggt aaacgccatg
    60781 gcgaaagaca tcatggaaga agtagccaat aagaacatga tcgtccctga cattgctact
    60841 gattgaaaca acatattagg tatatctttt agttttaccg ttttatagta aaacatagca
    60901 atcaacaggg agtaaatgac agctatcgct gaagcttcta ctgctgtaaa tatccccgtc
    60961 aagataccac cgataatgat gacaatcaat agaaggctag gaatcgcctc ttttattatt
    61021 tttccagcat gagccatctg tcctttttcg acaacagggt atttattctt tttagcaacg
    61081 atgaatgcta ctagcatgat tcctagcgcc cacaaagcgc ctacgagata acctcccata
    61141 aacaaagcag agatcgaagc accaccagca acaagtgaat acatgataaa agcagtactt
    61201 ggaggaatga ccatacctgt cggagctgac gcgatattga ccgctgtcgc aaacttccgg
    61261 tcatatcctt cttccacttg ctgagggatc aacactccgc caatcgcggt agaagcggca
    61321 atggccgagc tagagatgga accaaataaa gcattcccta acacattcgt atgagccaaa
    61381 gcccccggca cgcgcccgac gaacaacatc gcaaaatcca ctagtttctt agctatccct
    61441 ccattattca tgataatgcc cgagagaacg aaaaacggaa tagcgatcag cgaaaaacta
    61501 ttgatacttc ccaccatttt ttgagctgaa ctaaaagcag aaacatcaaa cggtataact
    61561 aacaataaag tcatcatcga tgagaatgca atacttatag caatcggcat ccctaataca
    61621 agcaacacaa caaatacaaa aaataacact aatccggctt ctattgccat tttattcctc
    61681 catttttata ttttttcttg aattgctggc ttgttgctta atgtatcgat tagattgatc
    61741 aggctataga taatgatgag tacccccgat acaggtactg acagataaac atatcccata
    61801 ggaatactta aagaaggcga gatctgcccc attgtcaatg tgacagcatt ccatccgcca
    61861 tacaccatga tcatcgcagc aaataccaga aataaacttt gaacaacaac ttctaaaatt
    61921 ttactttttt tgccagttaa tttttcactt aaaaccgtta cggctaaatg tgctttttgt
    61981 cccacaacat acgcggaaga caacatggca aaccagacaa gaccgaatcg aacaagctct
    62041 tccgtgaccg tatttggatt tcctaaaacc gttctggaaa atacttgcca taaaacaaca
    62101 gcgaccatcc caacaagcaa aacagctccc aggatctcta ataccttgtt taaaatcatt
    62161 ttcatcacag ctacgctcct tgctcttgga tcttttgata aaggtctttg taggtaggat
    62221 cgtttttgaa tatatcatga agtggggcaa ctgcctctcg aaacggtgta ttatctacat
    62281 cattgaattg gactccatgt tcttcttgtg ctaaagcttt ttcttcttca attgcttttg
    62341 caaaagcttc aatttcaaaa accgttgatt ctttcgctgc ttcttcaatg gctttttcct
    62401 gctcatcagt tagctgttct ttgatggcat cattcatgat gatgatatct ggcacacgtg
    62461 tatgctcatc ataggaatag tatttagcca caccaccatg tccagctgta tacaagacaa
    62521 attcattgtt ttctgatccg ttgatcaaat tggattgaag agacgtgtac acttcatcac
    62581 ttcccatagg tacgggcgaa ccgcctaaaa gctctaccat tttgatagcg gtttcgcttt
    62641 gcatcacacg gatcttcttt cccttcaaat cttccggcgt atggatcgga ccgtctacca
    62701 tataaaagct gcgctgacca gaatcataat aggtcaatcc ggtaaatcct aaatttttgg
    62761 tcgattggta gattggctcc attatttctt cattatccat caccttaaag aaatgttctt
    62821 catcattgaa tagataagga actgaaaaaa tcgaataatc tttagagaaa ctttccaacg
    62881 ctgaggcact tacttttgtg aaatctactg ctcctgactg tgtcaattcg atcagttcac
    62941 gttctccacc cagttgtcca tctgggaaat actggatctg tacggaacca tctgtttttt
    63001 cttttactaa acgcccaaat tcattcattg catcaattac tggctggctg ttgcttgcat
    63061 aagcaaagcg caaggtaact atctcattta catctgcatc tgcgacagtt gccacactag
    63121 aattatttcc gcagccgctt agacctagca gacctaggct aagcgaactt gctgcaaaca
    63181 gcagtgatgt ttttttcata tgggtttcct ttcttttaac aataagagac gagcaattct
    63241 gtcaaacaaa gcaccgttaa cgactgtcca taaggcattg ctgtgattcc gatattccga
    63301 taaaaatcta aactgtcccc cattcctgtc ccgattgaaa cgttttgaac ttctcctttt
    63361 tcatcgattt gttcaagcaa tccttggata gcacgataag cgacttcttt atattcttgt
    63421 ggtaaataac gtttatggac cgctttcaaa ataccgtaag caaatcctgc agttgcagaa
    63481 gattctacgt aagaagaagg atcatccaac aacgtatgcc ataaaccgct ttcagactga
    63541 taagttttta atgcacgcac ttgtgcttca agtgtttcga tcaataattt gcgcaatgca
    63601 tcttctttcg ataattctaa gatttcaatg atctctggaa tagcaatcgt caaccagcag
    63661 tttccacgag cccaaaatgc ttcggcatag ttatgatttc cttcaaacgt ccagccgtga
    63721 taccacaatc ctgttcgttt atccatcaag tatttgatat ggatcataaa ctggtaacga
    63781 gcttcttcta aatattctgg gcgattcaac agtttcccga tttttgccaa aggaagcact
    63841 gtcatcatca atgtatcgtc ccacaactga ttcttatttt ccggtccata tgtggcatgc
    63901 tgtaacccgt cttcatttgt ccgaggcatg tcattcatga cccattctgc ccattgttct
    63961 aaataaggca aatagcgtga atctttcgtg tcttcataca agtaagccat agtcaatagc
    64021 ggtgccattg tattgacgtt tttgggcgga gcgccttctt ccatacgatc ctcaaaccat
    64081 tcattgacca cttgataagc gcgaggattt tttgtcatct ggtaattttt ataaatacca
    64141 tatagaccga caccttgtgg ccaattccac actgtccagc ttttgtcatc tactttcagt
    64201 ccatcaaagt ccaacaaaaa ttcaccatta tcgtctttga tcgttgtcag gttttcaacg
    64261 agcaggtcaa ttttttcttt cactaattct ttaggtacaa aaatctcttc tttaataatc
    64321 atctttacgc caccttttta aacgctttca ttttatatcc ttaatatagc agtaataccg
    64381 tccactataa atatcgcttt ttgaacaaaa actgtctaaa attgacctaa atgttaaata
    64441 acaaaagtaa ctttcagtta cttggaaatt aaaaaaagaa cttggagaaa caacgttttc
    64501 caagttcttt ttcatctatt ttcttatctt ttttctacgc tttcttttgc ttatccgttg
    64561 taagcatcaa tgatgatctg tttcaattca gagatcaatg gttctttcgg attagctgtc
    64621 gtacattggt cttcgtaagc taattcagcc atacggtcta cggttgtatc tagtgtttct
    64681 tgtgtgactc cttgagcttt tagactcatg ttgatgccga cagattttcc taattcataa
    64741 acagccgttg ctaaagcttc gactaattcc tctgttgttt cgcctttcag acctaagaat
    64801 tttgcgatat ctgcgtaatc tgtatctgca cggaaatagt catatttagg gaacatcgca
    64861 tgtttttgtg gatcttttgc attatagcgg ataatatgcg gtaaaagaat cgcatttgtc
    64921 cgtccatgag ggatcccgta ttccccgccg attttatgtg cgactgagtg acaaatacct
    64981 aggaatgcgt tagcaaacgc cataccagcc attgtagaag cgttgtgcat tttttcacgt
    65041 gattccatat caggtgtttt tactgatttt tctaaatagt caaagactaa cttgattgct
    65101 tgtaggctca atccacgtgt ataatctgaa gccatgactg aaacatagga ttcgattgca
    65161 tgtgttaaca catccatccc ggtatctgct gtgacagatg ctggaactga catgacgaat
    65221 tgaggatcga cgattgcgac atctggcgtc aatgcataat ctgctaacgg atatttcaca
    65281 tgtgtttcac tgtcagtgat gaccgcaaat ggcgttactt cagaaccagt ccctgatgtt
    65341 gtcgggatac atacgaattg tgttttttct ggtttgtcga tcttatatgt ccgtttacgg
    65401 atatccaaga atttttgttt tgcaccaaag aaagatgtgt ctgggtgttc atagaacatc
    65461 cacattccct tagctgcgtc cattgcagaa ccgcctccaa gagcgattac tgtatctggc
    65521 ttgaagtctg ccatcagttt cgttcctgcg tatacggtat ttgtcgatgg gttcggttca
    65581 acatctgaga agacttcgat cttcacatca tttttacgtc gtgccaatac ttcacgaact
    65641 gtatcgcagt aaccgaattg gaccatacca gggtcacaaa cgatcatgac acgttcaacg
    65701 ttttccattt tctctaagta caatagagag ttcttttcaa agaagatttt tggcggtaat
    65761 ttgaaccatt gcatgttatt tctccgtttc gctacagttt tgacattgat taagtttaca
    65821 gcagagacgt tacgagaaac agagttctta ccataagaac cgcatcccaa agtcaatgac
    65881 ggaatcattt cgttatagat atcaccgatg ccgccttctg cagatggtgt attcactaaa
    65941 atacgacaag ctttcattcg aagaccgaat ttgacatgca actcttcgtt ttctgaatgg
    66001 ataactgctg tatgtcctaa tcctccaaga ttcaacatac cttcacacaa atcaaacgca
    66061 tggtccgtgt tgttagcttt gatcatggca agaactggag aaagtttttc tcttgatagc
    66121 ggataatctg ggcctacgcc ttccaattca gcaatcagca ttttcgttcc ttttggtaca
    66181 ctgattcctg ccaattttgc gatttccatc gcagaatgtc caacgattga tggattgacc
    66241 gcatattttc cttcgttcat cactgcatct tctaattttt gcaattcatc tggttttacg
    66301 atataaactt ggtgtgcttg gaattctgct tttactgctg catagatttc tttatccacg
    66361 atcacagctt gttcagaagc acaaatcatt ccattatcaa atgttttcga aacgatcaaa
    66421 tcgttcactg cacgtttaat ttttgctgtt ttttcaatat aagctggtac gttacctgga
    66481 cctacaccta aagctggttt tcctgtagag taagcagact tgaccattcc agctccacct
    66541 gtcgctaaga cgatcgcgat acctggatgg ttcattagca tagaagtcgc ttcgatcgaa
    66601 ggatgttcga tccattggat acaattttca ggagctccgg cagcgatagc agcatcacgt
    66661 accactcgtg ctgcttctgc agaagatttt tgggcacttg gatggaaagc aaaaacgatt
    66721 ggatttcttg ttttcaacgc gatcattgat ttgaaaatcg ttgtggaagt cggattcgtt
    66781 gttggagtta ctccgcaaac cacaccgact ggttcagcaa tttcaatcaa ccctttttgg
    66841 acatcttcat tgatcacacc aactgtttta tcatgtttga tattatgcca aatatattcg
    66901 gacgcataca tatttttgat tgctttatct tcgtaaacac cgcggcctgt ttcctctaca
    66961 gccatcttcg ctaatggcat atgttgatcc aaagctgcca ttgccatttc atgcacgata
    67021 tggtctactt tttcttgatc aaagctttcc atttctttca atgctgcatt tgctttttct
    67081 gccaaatcat caatcattgc ttgtacgtca attgtcttcg ttttttcttt ttctattact
    67141 ttagccatca ttatcctcct aatgggtcat ggttatttgt taaataattc acatgtatat
    67201 catacaatat ttatttttct atgtaaacac ttttttgtga tttttttcac agttattttt
    67261 aaatataaaa ttgctttatt tacaggtgtt tgtatagaaa aatatataaa gttatttatt
    67321 attatttata tgacacaccc gattattcaa tattgagcaa tatatcccat gctcttatct
    67381 ctttcttttt tgaataaaaa atgaggtagg acatctttct gccctacctc gtctttaaat
    67441 aagctttgat acttattttt cttccttctc ttctgtctct tccagcgttt ctgttgtttc
    67501 agtttttact tcaactgtct cagctggttc ttctgttgtt tcaacagtag tcgttgcatc
    67561 atttgttacg acagtccctg gtttgaccgt tttgattgct gcacgatcgt actcaaggaa
    67621 gatcccttcg cagtctaaaa caactgtccg tttgtcattg tcgatttctg aaacaactcc
    67681 atgtaatccg ccgatcgtta cgactgaatc acctggtttc atgctatcta acaagttttg
    67741 acgttcttgc tgttgtttct tttgcgaacg tgacataaaa aaccacattg ctaatagcgc
    67801 gataaacatc acaagcattg gtaatgttcc catttttttc acctcactaa ttctgtatct
    67861 acaaaaaaca ctttagcaga aaaatgctga taacactagt tttttactta atttttaaaa
    67921 gattttcctt tagaagcttt ttgcgttttc tttattgaac ccgtattctt caaaaaaggc
    67981 ttctctaaat tcaagcaaat tatcatccat gattgcttga cgtacttgtt tcattagatt
    68041 caacaagaag tataaattgt ggtaagaagt caaacgaatc ccaaatgttt catcacattt
    68101 gatcaaatgg cggatatagg cacgtgtata gtttttacat gtataacaat cacatttctc
    68161 atcaattgga cggaaatcat gggcatattt cgcatttttc actactagac ggccttgaga
    68221 agtcatacat gtgccatttc gagcgattcg agtaggcaag acacagtcaa acatgtctac
    68281 tccacggata acaccatcga tcaatgaatc agcagcaccg acacccatca agtaacgtgg
    68341 tttgttttcg gggatcaacg gtgtagtgaa ttctaataca cgattcattt ctgatttagg
    68401 ttccccgaca gataacccgc cgattgagta accagggaaa tccatcccga ctagatcttt
    68461 cgcgctttga cgacgaagat cttcaaaccc tgctccttgg atgattccaa ataatccttg
    68521 acggtctgga ttagcatgag ctttcaatcc tcgttctgcc catcgagacg tacgttctac
    68581 tgattttttc acgtagtcgt aactttcatc gaatggtgga cattcgtcaa aactcatcat
    68641 gatatcagag cccaatttat tttggatgtt gattgctttt tctggtgata aaaacatacg
    68701 cgaaccgttc aagtgatttt tgaaatgcac gccttcttct tcgatattcc gcatatcact
    68761 taatgaaaaa acttgaaagc cgcctgaatc tgttaaaatc ggttgatccc agttcataaa
    68821 tttatgcagt ccgccagctt cttctactaa gtcttcacca gggcgtagcc ataaatgata
    68881 tgtattactt aaaataacgc cagcacccat ttctttcaac tcttctgggg acattgtttt
    68941 tactgtagct aatgttccta ctggcatgaa cattggtgtg gggaaagttc cgtgtggcgt
    69001 gatcaattcc cctaaacgcg cgccagtatg tttttctttc ttgattaaac gatagcgaat
    69061 ggcaggttgc gtcattcatt tccctacttt ctatataaaa tatcttgaca actcccacca
    69121 tcataatcgt ttcaagtgct ttttgcaagt tccctcggct tccttctact ttatttaata
    69181 ttcttttttt tatccttttt ttatgaataa caaaaaaaag aaaaacatta tgacagaaat
    69241 attttttgat acatggtata ttggtgttat taaagaaaag gagggaaaaa tcatgcaatc
    69301 ttctgcagaa ttaaaacgcc aagcaaaaga gagtctaaaa ggacgttggg gacaagctgt
    69361 tttactaaat ctaatcccta cattgattgg tattgctact attcttctta ttatgattcc
    69421 tattatcttg tttgccacca ccatatatga tggatcagcg agcgtctcag aagcgacaac
    69481 ttcaggaggc ggtagttcag gaggcggtgg cggaattatt tccagcatca ttagcgcttt
    69541 gttcatgagc ggtatttctt ggacttatct tgatatttta cgtggacaaa aagatcagat
    69601 cgaaccattc aaagatgcat ttcgtggatt caaaggtttg tttatcggcg gagtgatcag
    69661 cttagctgtg ctgatctcga tctttacaac attatggggt ttgctattag ttattcctgg
    69721 aatcattaaa agctattctt attctcaaag ctattttatt tactacgata tcgtccaaga
    69781 aacaggacaa acacctaaag tactggatac gatcactgca agtcgaaaat tgatggatgg
    69841 ttataaagga caacttttct ggttagatct ttcatttatc ggctggcata tcttagcatt
    69901 agcaactgct ggtattggtt atctatggct cagcccatac atttctgcaa caaaagcagc
    69961 attttatgaa caattgccaa aagatattta aaagagagtg tgacttgtga ccgagggggt
    70021 ggcaggagaa caccgtttat cgagaggcag taaaagaaac attcagccca ttagcaaaaa
    70081 ggaggtgtga caacattcat gtcacacctc ctttttgtat agttccagta aaaagaaaat
    70141 caacttagaa acagttaatc attcctcttc ttctgcttcc ggtaatcgat ggaacttgat
    70201 cagttcattc accgcgattg gtacgataga taaaatccct accaagaacc agtgattcaa
    70261 actgatgtgt actaggccga atagttcttg cgtaaatggc agcaaagcaa cagccgttgt
    70321 aatagctaat gccagtaacg ccatttctac caaagcttta ttggtcgcta aattgacttt
    70381 gaagatcgat tggctgctgc gtacattgaa cacatgcaga atcgatgaat aagctaagat
    70441 caagaatgca acagtttgac cgacatgctg acttgcgtct acccatgggc tgatagtatc
    70501 gacataacta cctaaataat aaccaaacaa ggttacgatg gtgaagaccc ctgcatttat
    70561 ggcaattttt tgcaataaac ctcgagcaaa gatcccttca tttttcggaa tgggtggctg
    70621 atccatgatg ccgtgttccg ctggctctct tcctaaagca aatcctggaa gtccatcagc
    70681 aactacattg acaaacagta gttgcacagc tgtaaatggt gctccccatc ctaacagcat
    70741 cgcaatcaaa acaataaaga tctccgaaat attacagctt agcaaaaagt tgatagcttt
    70801 acgaatattc tgataaaccg aacgtccttg actgacagcg tctacgatcg tcgcaaagtt
    70861 gtcatccgtc aaaatcatat ctgcggcact ttgcgccaca tctgttcctg tgatccccat
    70921 ggcacaccca acgttacttg ctttcaacgc tggtgcatca ttcacaccat ccccagtcat
    70981 cgctacgact gcacccgtac gttgccaaga tttgacgatc cggatcttgt cttctggcgt
    71041 tacccgagca tagacagaga gagctttgac acgcgcatct aattcttcgt ctgataattg
    71101 atgcagttct gatcctgaaa gtgcttctga cggatcattc aagataccta attctttcgc
    71161 tatggcactg gcagtcacca catgatctcc agtgatcatg actgttttga tgccagcctt
    71221 tttcgctcgc ttgattgctc ctttactttc tggacgtggt ggatcgatca ttccgatcag
    71281 accaatcaaa cgtaagtctt tttccagtgc ttcagaagaa atatcttgcg gttcttcgtc
    71341 ataagtagca taaccaacag caatcactct cagtgcccgt ttaccaaagc gatcattaac
    71401 gatcgccgct tgttctacat cgcctgaact aaagcgcgga gcaagaacat cgaatgctcc
    71461 tttggtgatt gagatgtatt tttcgcccat ttggtgaact gttgtcatca tttttcgttc
    71521 agaatcaaaa ggaagctcgt ttactcgtgg atatttttct tctaattctg cctttgtatg
    71581 ataattctct tcaacagccc ttacgatggc tacttctgtt ggattacctt tgataacaag
    71641 gtcgtcatct tcttgctcga ccgtcacatc tgtacatagt gcagccattt tcaatatttc
    71701 catcgcgctg ttcgtcattg aatcttcgat attcgttact tcatcttctt tgctccatac
    71761 ccggcggacg cgcatttcat tttgtgtcag cgttcctgtt ttgtctgagc agataacatt
    71821 agcggttcct agcgtttcaa ctgctggcaa ctgtcggatg atcgcatgtt tcctcgccat
    71881 cttctgcaca ccgtatgcaa gtgtcaacgt aacaataaca gtcaaggttt ccggtacagc
    71941 tgcaacagcc aaggaaatcg aagtcatgaa catctcaagg agtggttctc cttgcagttc
    72001 accaatcaca aatactaaag ccgcagccgc taaagcgatc atactgattc gtttgcctag
    72061 ctgattcaat cggacctgta aaggtgtttt ttgcatagta ttttcgttca aaagatccgc
    72121 gatcttaccc atttctgtag ccatgccaat ggcagtgacg atcgctttcc cacgacctgc
    72181 agcaaccgta catcctttaa ataccatatt aagttggtca cctaatgact catcttcttc
    72241 agcaataaat gctgcatctt tttcaactgc ttcactttct ccagtcaacg ccgattcttc
    72301 tacacgtaat tgtgatgctt cgatcaaacg ggcatcggca gaaatagcat caccggactc
    72361 taaaacaaga atatcaccca ctaccagttg ctctgactca acagtggtca ctttgccgtc
    72421 tcgtatgaca gttgccgttt gtttattcat atcttgcaag gattccagtg ctttttctgc
    72481 gtttccttct tggacaatcg ccaataccga gttgatcaca acgatagcga tgatcagcag
    72541 tccttcaaat aaatccccat gttctgtagc aaatgcggta taaaaggaaa tcaccgctgc
    72601 tacaagaagg atgatcgttg taaaatcgga caagcttcga aagaattttt tgatcatcga
    72661 ttctttcggc gcctcatcga atttattcaa tccctgcttt tttcgccgtt catccacttc
    72721 ttgtgaagac aacccagtat ctggatctgc ttctgttacc ttctttactt cttctattgt
    72781 ctgcttgtat tcttccatcc tattcctcct ttaaaatctt cgtacaaaaa aaggtactta
    72841 ttttactata acatcctttt tttatttact tcaaataaag ggcttatcga ttatttgtta
    72901 agaaagcgta aaatttaaag tgcttatatt tgcaaacgct accaaaggat gctattatat
    72961 agatgtaagg aaggaggata ccatcaaata gacagaagaa tttctcgata gaacagtgta
    73021 agcaaacaaa gttgttagaa gtacaggttt cacccagaca tgcgtgtaga gtacaaacct
    73081 ataaatagtg aatgcgtatg gatgtttgga tcacgaagta tccgaaacaa gaagtaatgc
    73141 gatacgtaac aattgaataa cgtaaacgtc tgaaaattga ataggaacaa gagatgtttc
    73201 aaatgttgaa gatgtaacga aaacaaaatt tttgtttaac atgaagaaat tcaacaccgt
    73261 gctttgcatg tatgatggaa agttacaatc atttctgttt atttgaatcc ttccgaggta
    73321 caagaaagcc gagcttatcg agagctcggc tttttctttg tcataaaatc tttgtaaagc
    73381 ttttttgtta ttcaaaacta gcggcttttc ataaccttat ttcacgaaca tcgcatctcc
    73441 aaaactaaag aaacgatagc gctcttcaat cgcatgctgg taagcagata aagtcaattc
    73501 tcttcctgca aaggcactaa ctaacatgac taaagtcgat tttggcaaat ggaaattagt
    73561 tgagaaagct tctaccactt tgaattcata gccaggcgta atgaaaatat cagtccatcc
    73621 gctgtctgct ttgatttctc cattaaattt cgttccaatc gtttctaatg ttcgaattga
    73681 agttgttccg acagccacga ttttcccgcc atttttacgt acttggttca atcgctcagc
    73741 cgcttcttcc gtcaaacggt agaattcact atgcatatgg tgttcttcaa ttttatctac
    73801 gctgacaggt cggaatgtac ctaaccctac atgtagtgtc agatagacta attcaacacc
    73861 ctttgcttcg atctcatcaa gtaattcttt cgtaaaatgc aatccagctg ttggcgctgc
    73921 agcagaacca ttttctttcg catagacagt ttgatagcga tctgggtctt ccagacgttc
    73981 tttgatatat ggaggcaatg gcatttctcc caatgattca agaatctcta gaaaaattcc
    74041 ttcatactca aagtcaatga tccgtccgcc gtgttccaat tcttctttca ctactgcttg
    74101 caatctgcca tcaccaaaag agattcttgt cccaactttg gcacgtttcg caggtttgat
    74161 caatgtctcc cacgtatctc cttgtgtatt cgttagaaga aggacttcta aatgcgcacc
    74221 agtatctggt ttttctccat ataaacgagc aggcagaact cgtgtattat tcataactag
    74281 cgcatcacca ggctgtaact cttcaatgat ttcatggaaa tgtttatctt ccatctctcc
    74341 agttttccga tccaaaacaa gtaaacgaga atgatcgcgg tcttttaatg gtgtttgtgc
    74401 gattaattct tccggcaagt caaaatcaaa atcttctgtt gttaacatat cgtcactcac
    74461 tttctatcat gtgcttcatc attctatcat gtttttcgtt ctgttggcaa aatcagcact
    74521 ttttcacttc tattttttgt tttcctaaat aaaaaaacta ccctatttgt attttttcat
    74581 tgttttaggc atactagtaa tagatagttc ttcattgaaa atagaaaaag aggtggcacc
    74641 atgattgaat ttcaaaatgt gtcaaaaatt tataaaggtg gaaaagtcgc tgtagaggac
    74701 gtcaatcttt catttgaaaa gggtgagttc atttgtttta tcggtacgag cggaagcgga
    74761 aaaacgacga cgatgcggat gatcaaccga atgactgatc caacacaagg gaaaattttg
    74821 attgatggaa aatccatcca agaaattgat ccagtggaat tgcgacgtca gatcggctat
    74881 gttatccaaa gtattggatt gatgcctcac atgacgatcc gtgaaaatat cactcttgtc
    74941 caaaaattgt tgaaagtcag tcaagaagaa cggaataaaa cagctgaaaa aatgattgat
    75001 cttgtggaat tgccccgtga gatgttagat cgctacccac atgaattatc tggtggtcag
    75061 cagcagcgta ttggagtcgt tcgtgccctt gcagcgaatc aagatatcat cttgatggac
    75121 gagccattcg gtgcgcttga tccaatcact cgtgattcct tgcaagacct tgtcaaagat
    75181 ttacaagagc gtttgggaaa aacgatcgta tttgtaaccc atgacatgga tgaagcgatt
    75241 aaactagcaa gccgtatcgc catcatgagt gaaggccgtg tgatccaatt tgatacacca
    75301 gaaaatattt tacgtcatcc tgcaaacgat ttcgtagaag agttgattgg agaagatcgc
    75361 ctactacaag ccaagccaaa tgcgacaaga gtcggcgaag ttatgctaaa tacagcaatc
    75421 acgattacac cggaaaaatc attacaagaa gcaatcaaac tgatgaggga aaaacgtgta
    75481 gatacgttgt tagttaccga taattcgaat gtattaaaag gatttatcga tgtagaaacg
    75541 ttgaataaaa aacgaggaaa agtatccagt gtcggcgaca ttttgaataa acatgttttt
    75601 tacgtaaaag aatccgccta tcttcgtgat accttgcaac gcattttaaa acgcgggctg
    75661 aaatacgtgc cggtcgttga tgaacagaac aaagtcgtag gtatcctgac tcgtgcatca
    75721 ttagtggata tcgtctacga tgtgatttgg ggagaagatc ccgcaactac tgatgttgca
    75781 gagtcagcta ctcctgaaga atctaaggag gtctgaccta tgcagcagtt ttttcatcag
    75841 cacggttcag aaattctctc aaaaggtttt gaacatcttt atatttcagg acttgctttg
    75901 atcattggta tcatcattgc tgtaccgctt ggtgtcttac tgacacgttt tccaaaagct
    75961 gccacgattg tgattggctt aaccagtgcc ttgcagacaa tcccttctct tgctttattg
    76021 gcattgatga tcccgctatt tggcgtaggt aaattacctg cgattatcgc actctttatt
    76081 tattcattat tgccgatttt acgtaatacg tacattggga tgaaaaatgt cgattggaat
    76141 tatcgggatg tggcaaaagg catggggatg actgagttcc aatccatttt ttccgttgaa
    76201 ttgcccattg ctatgccaac gatcatggct ggtattcgtt tagctgccgt ttacgtgatt
    76261 gcatgggcta cacttgcctc ttatatcggc gctggtggtt taggcgattt gatttttagt
    76321 ggactgaata attaccaacc agatctcatt ttggcaggta cgatccctgt aacaatttta
    76381 gcactattgg ctgattggct tctaggtctc ttggaacacc ggctgacacc gatcgcactt
    76441 agagaggaga atgaagaatg aaacgtatac taaaaatgac catggttctt ttctctcttc
    76501 ttatacttgc gagttgttcc ttccctggtc ttgccagcaa tacagatgaa gacaccattt
    76561 cgatcacagg tggaatcact tcagaagcac agatcttagc aagtctagtt gccggaatgg
    76621 ttgaacatta tacagataaa aataccgcaa tcatcaacaa tctggctact acaactatca
    76681 accatcaagc gatgatgaac ggagatgctt caatttcagc ggctcgctac acaggaacag
    76741 atttaacgac tacattgaac cttcctcctg aaaaagatcc gaaaaaagcg tttgctaccg
    76801 tgaaagatga atttgagaaa agatacggtc aaacgtggtt cccgtcctat agttttgaaa
    76861 atacgtatgt ctttcttgtt cgaaaagaca ccgcacaaaa ataccatcta tctaaagtca
    76921 gcgatctgaa aaatgtagcg gatgaattag tagctggtgt agatacttct tggatcaatc
    76981 gtaaaggaga cggttatgac ggcttccaag aaacttatgg cttctccttc aattcgatat
    77041 tacctatgca gattggactt gtttatgacg cagtcgaagc aggaaaaatg gatattgttc
    77101 ttggctactc tacggatgga cggatcgcca gttatgatct agtcatgttg gaagatgatc
    77161 gtcaattctt cccaccatat gatgctgctc cagttgtaga caacaagata ttagctgata
    77221 cgcctcattt aaaagaagcc ttaaataagt taggtcatac catcagtacg gaaaaaatgc
    77281 aggaattgaa ctacgaagca gataataatc tagtggaacc atcagtagta gcacaacgtt
    77341 tcttacaaga aaaccactat tttgaaggga agtgaagcaa ttggataaaa tgaatgtctt
    77401 tcaacaattt ttctattatt tccaggaaaa tggctcctat atctttgcac aatttatccg
    77461 tcacttcctg atttctatct atggtgtcct ctttgcagca gttgtcggga ttccggttgg
    77521 gatcatgatt tctaggagac gtaaattagc aaactgggtg attcgtttgg cgaatatcat
    77581 ccagacgatc ccttctctag ccatgatctc cattttaatc attggattag gcctgggagt
    77641 caatgtcgtg attgttactg tatttttgta ttccctctta ccgatcatca agaatacgta
    77701 taccgggatg atccaagtag acaaaaacat ccttgatgtc ggcaaaggca tggggatgac
    77761 tgctagacaa cgactattca tggtagagtt accactctct gtttctgtta tcatggcagg
    77821 cataagaaac gcgttagttg ttgccatcgg gataaccgcg atcggtgcat tcgtcggtgc
    77881 tggtggtctt ggagatatca ttatccgagg aaccaatgca acagagggca catcgattat
    77941 tttagcaggt gcattgccaa ctgcattaat ggcgatcatc acggactggc ttttaggtat
    78001 tttggaaaga cggctagatc cagccagccg tacatcacgc tgatgttatt gtaaaaaaca
    78061 aaagcttctg cgaccatttg ttggtcgcaa aggctttttt agttctgtca tttttaaggc
    78121 aataatccac tctgattctt ccctccttat gggcaaagag ttgcttttct tagctattta
    78181 tccattccgt atactccaac ttcatactgc caacgggttc atatctgcat ccaccgacta
    78241 agtttttttc ttatccgcta aggcattttg ccaatccatt ttcccatcac tcgtcatgga
    78301 attcatacca catgatctct aaatactcgt ctgcttgttc tttgaattgg gcatcaattc
    78361 ttacatcctt tggcgtggct ttcatttaag ccttttccat cagtcctcga ttgttcgatg
    78421 ccttcagctg catcatcaaa actggtgact gccgtatcgg taacttcgat catcttttat
    78481 acaatcggga acttgccatc ctcatccttg gctaaaaaca tatctagcca ttccagcttc
    78541 tcgccatggg aggtttgcgg ttcctaaaac cggtacacga ttcttatgga ctgcatccgt
    78601 cgcaactaga cttggcgata cgatgatcct tcaccagaag agcacccccg taaaccaatt
    78661 gatcgatata ttgccaataa gaaaagacat tggcgtaaaa gatatcgatc cctcgtgatg
    78721 cattgccgtt gatactgcta ttcatggccg aaagcgccac gactttaact tcttccgttt
    78781 gagtgacatt catgttcggc aatgtcgctt tggatacccg ctctgctaaa ggaacgacac
    78841 tggtattgta tttcgtatct gtatcttttc caaaagacca tgccaacgaa tcctctggga
    78901 accaataaga cgactccggt tgattgtcca ttgctttttt caacatactt ttggctgttt
    78961 ctcgataaac gacatttcca attgtcttcg cctcactttg gcgataatcg atggtcgttg
    79021 ccactgccaa cactaacaac gatgctccaa ttttactgat tttccttgtc acacttcttc
    79081 ctccttgttt tacataaacc cgtcaacatt ccattcctaa actcaaacaa tgacccttca
    79141 aaggtggaga tataggaaca aattaaagaa atcatgcaat aaatccaaga aggttggttt
    79201 tagaacaaaa aaagagtctc ctttttgggg agactctttg ttaactatct aaaaaggttg
    79261 gctcttctca cttcactcat aatgtgtcca ggcagaataa atttctacaa ctttattttc
    79321 ctcatcaact ttgtaaacca ctcgatgctg tctattcagc ctgcgtgagt atctgccttc
    79381 atgtgtaggt ctcagctttt caaaagactg cgtgggcata tatggatctt ccttcaatgt
    79441 ctgaatcact tcttcaaatt gtttctttaa attcgtttgc ttgatttttc ttaaatccac
    79501 tttcgcagag tttttgattg cgactgtata gttactcatc aaagagcttc ccaatcaata
    79561 tcatcgatat tcgtaaagct actgtcgtct ttttcgcggt ctcgtacttt atctaaagta
    79621 cctgtttgct caagaaaaag ggtttcttga atcgctctcc agtcttccag cccaatcaat
    79681 acagcattgt ttccactacg atcactaata atctcgattt cagtatgatt ttcatttacc
    79741 tctttcaata actgataaaa atttttacga gcagtgctag gattcatcgt tgtattagcc
    79801 attgtctctc ctcctttttc tgatataatt atagcgtact tgattaagta cgtcaaaaaa
    79861 atacaataaa gcacttaaat tcagcaaact tttcctcttt tttacataga gaaaaagacc
    79921 cctttttagg gaggatcttt gtccacgctc acttcttact cctttacgac tagcggtagc
    79981 caaacgtaaa gtaattggta acaacgtatc atctttagca aaggtcgaat ccaaagcctg
    80041 tgagtctatg caacaacacc aatacacgaa ggttcctcgt tgcagtgtga cttgattgat
    80101 atcttgaata acctgaggat acgcacgaaa gatttgtaac ggttattcaa aactgatttt
    80161 ggccgtcaag tcttctactg cctcaaaaat gattgcctct ttcgtctcct gtcattcatg
    80221 ttcactatcg atttgatact tgcttagacc gttgggtttg tgaatcacca attaaaaatg
    80281 gcactgagtt atggtaacaa aaaatggtca cttgacgagg ctgagattcc tacttatgaa
    80341 gacacatggc acttaagagt cgaggtaaag gaagaagctg aaaatatagg aggctgtaca
    80401 ttatttggca aaggattcgg aaattatgat caagacatca aaatcaaaat gttttacagc
    80461 taaataggaa gaataataac atttcatacg ctgttcccat ctttaatggc ttatagaggg
    80521 cacctttagc tgagaataag acaaaacaga ccctctagct tcaattaaga gctagagggt
    80581 ctgtttttga ttttaaaact tggtttattt cttcaaatgg tagctatagg tgctgacaac
    80641 gatattttca cgccaagaca atcgcaatct taaccgcaaa cttgtttggt tatggagaat
    80701 attctgccag cgtctgtttg gtacaaattg aggaatcaac acagtagttg tatagttttg
    80761 tttcgctgca tttttactta cgatatctac ataacggata attggatttt caatcgagcg
    80821 ataagaagag tggacgattg aaaaacggac atctgggaaa tgctttttaa attctgcttc
    80881 gatttctttt tctttttcga catcttcgtc tagcgaaaca tgcatcgcaa cgacataatc
    80941 accgatcgaa cgtgcgtaat tcaacgcacc gatatttacc cgtgtcacat tcccgactaa
    81001 tacaagaacg gtattcccgt cataatcatg tagttttgca tcattttcta aacgaagctg
    81061 ttctgctact tttttataat gatcatggat cttataaaaa atgaacataa cgattggcat
    81121 aatgatgaag aaaggccaaa tgtcccctaa ccgatagaca aacaaaatcg caatgattgc
    81181 atatgagatg aacgcacccg taatattggc aatcgatttg cttaaccatc tttttccttc
    81241 ttttttccat ttgacgacca tccctgtttg agataaagca aatggaatga acacgccgat
    81301 agagtataac ggaatcaagc gttctgttga gccatggaaa ataaacagca atacgatcga
    81361 acctagcgct agtgtgataa tcccattgga atagcctagt cgatccccac gatcttgata
    81421 catgtgaggc atgaatttat cttttgccag attgtatgca agaactggaa aagcagaaaa
    81481 accggtgtta gcagcaactg ctaaaatcag cgctgtagca aattgaagga cgtaatacaa
    81541 aataccatgt ccaaagacag ctttaccaat ttgagacaaa acagtgactt ctttttcagg
    81601 aacgatccca taccaatagt tgataaatgt gatccccacg aagaagaaac ctaagatcaa
    81661 tgccatcatc gtcaatgtag cagcggcatt ttttgctctt ggcttcttaa agaatggtac
    81721 agcattacta atcgcctcca ccccagttaa agaggaagaa ccagaagaaa atgctcgtaa
    81781 aatcaaagcg atagaaacac ccggcacagc cgctcctgga agtgcagtag cttgcaatgg
    81841 cacagcacct gtcacgatat tatataaacc aacaacgatc agtaacgaga tcacaaggat
    81901 aaatgtgtac accggaaata ataggaacga tgcagattcc ctcagcccac gaagattcag
    81961 catcatcagt agcaaaacga ttgtcacaga aatagcgact tgatgaccgt agagtgctgg
    82021 caccgctgat gtgatcgctt cagcacctgc tgagacagat actgctactg tcaacatgta
    82081 gtcgatcaat aaagagccac cggaaatcaa gccagcattc tttccaagat tctcactgct
    82141 gacgacatat gctcccccgc catgaggata agcatggatg atttgacgat atgatagcgt
    82201 taatgaaatc aataaaataa taacaaaagc ggcaatgggt agcgaatacc agatggctgc
    82261 agcagacaaa gcgactaaaa cgacaacgat ttgttccgta ccatatgcaa tagatgataa
    82321 cgcatcagac gacaataatg ccaaagcagc aaatcgggtt aatttgtgct catcattttc
    82381 agcagatttt aaaggcttac ctactaacaa cctttttaag taatccaatt tctaaactcc
    82441 tttacacaca attttacttc aagttttata gtcgaaaatt tatagtaaac aagatgttat
    82501 agtacgctca ctgaccttaa aagtcaataa actttaagaa aataattgga aaaaaacaga
    82561 aaaatatcaa gcgctttttc aaacgcttta atacagcaaa aatctaacat ttttttatgg
    82621 gctttttctt tgaattctat aataaaagga tagaacttga cttagaaaat caagttctat
    82681 cctttttgat atcttaactt ttggaagaga gatgtaaggc tgctttctac ccatcattct
    82741 tactactcga agcatagcgc agttgtcaca acctcttgat aaacggtgtt taaaagcttg
    82801 catctgcggt aataagccca accaaattaa aaacataaga agctgttttt atttggtcgt
    82861 tcttattact tgatgcagag cagctttttt acaacctctt ggtacacggt gttcaatcag
    82921 ctgaccctcg gtattagctc aaaaaaacgg caaaatatga ggaactattt ttccattttt
    82981 ttctctaata ctcaggtcaa aacactgatt tcacaacctc tttttttaat gaaaaatatg
    83041 gctctcatgc gccgcttctt tgaatttcgt cattttttct tcgatgaacg gatagattgc
    83101 cgttcctacg attccaaaga caacaaacaa accgatcatg atccagattg ctttcgtagc
    83161 atttggccaa taaattcctc ctgcagattc ccgtaacaag tttaccgcat gagtaaatgg
    83221 caagaacgga ttgatcattt ggaagaattt ccctgagact tggatcggat agttcccgcc
    83281 tccgccagag atcgacagta ctaagatgat aatagcgatc cctttaccga cattatcgaa
    83341 taatgcagcc aagacataga cgatcatcat gaacgctaag gcgatcaaga cagcaaatga
    83401 cacgctgtag accggatttt ggacatctac tcctagtaag aaaatattcc ctaatgtaac
    83461 gatcaagctt tgcgcaatcg ccattgtcaa aaatgtcagc atacgagcaa cgaattgttc
    83521 tcgtttcgta aaattgtttc ggtctttctt tcctaaatag tattctgtcg tagcaacact
    83581 tgaaagaaga acagcgccga cccacagaca tagtgcagta taaaacgggg tgctagctga
    83641 accgttattt gcgattggat aaagcgcatt ggtttgtagc tcaacaggtt gtgtaaagaa
    83701 gtcactttct tttgttgcgt ctagtttcaa taatttgatg acttcgccta gatcgacttc
    83761 ttgttcgcct tttcggatcg cgtctgctgc tttttggata ccagctttga tatttggcca
    83821 gtcattgttg atcaagtccg cagcagcgtt cacagctgtt tcgacttctg gcattttttc
    83881 atttactgtt gtcaatgtac cggtgatttc attttttagt gctggccaat cattttggat
    83941 aaaatcagcg gccaatccta atttttgctg aatgatcggt aattcattgt tgtataggtc
    84001 tgccccttca ttgatgccat tgacgatggt atccatattt ccattcagca ttgtattggc
    84061 atcatggatt tcttgtccaa ttgcaggcat ttctttttga tatttttcta aaattgccac
    84121 tgcattcgtt acagttgctt tcgttgagct gagaagcgaa tcaaagtcga tctggtccgc
    84181 tttgctcagt gcgccttgtg cagtcccaat cgtatcgatg acttggtcta gtagagaagt
    84241 gacagtggaa ctgatttgac ctgtattgat accagcaatg atgccattga tattattaac
    84301 aacggcatca atttgatcta gcaattcatc aatagccttc gtgtcttcat tttgaacagc
    84361 tgtgttcaaa gcagataggc ggttttgcag attgcttagc aagggtttta attgatttag
    84421 ctgatcaata attggctgta atttcgtatt tccagcactt gtttgcaatt tagtcaggaa
    84481 ctcgaccagt ttatcgattg cattttgttg ttgagatatt ccgtcaataa agccgttcaa
    84541 tacattgctg atgtttttct tttcatcatc agtcagctta ccatcttcaa tagctgttct
    84601 tacagtatct attaacgaac ctgtcgttgt tgccacttgt ccaattgact tcaatgtaac
    84661 atctacactg tttgtgatgc ttggtatcgc atctttcagc tgagaggcac ctgattttgt
    84721 ttggtttgct agatcacttg cctgattttc taaatttttc acatctggca agatatcttg
    84781 tacttgttgg atgatcgtca agccttgttt tgcttcatcg attccttggt tcattgtctc
    84841 ttggatcgaa gcaaaatcgc cgtcgatctc tgctagttgc ttaccggcat tttggatctc
    84901 tgggattttt tcttgcaaat caaggatgat ttttgcttga tctttcaact caggcatttt
    84961 gtcattcaat gcaacgactt tttcacccat ctcatctact ttagggatgt aatccacgaa
    85021 ttcatttgct ttattcagtt tttccttgat ttcaggtagt tgactttgta attcgaggac
    85081 ttgttttgtg tagccgtcga tcgtgtccaa attttcatct gttgataaaa tcatatcttt
    85141 aactttattg atacttacta ggttcgtatc gatgtcgtaa cctatctcat tgaatacttt
    85201 caataaagtc gaactggctg ttttgataaa ttcatttgtg atttgcgatt ggatcgaact
    85261 tgctccttta tcggtgattt tcggtgcaat cgcattgatt ttttcattta ccgtatattc
    85321 gatttttggt ttcttgatat caccactagt aaaactcagc agatcttctg agaaatcctt
    85381 tggcaagtaa atacctgcgt aatactttcc ggaacggact ccgtcttcta attgttcttt
    85441 tgaatcaacg aattgccatc ctaattgttt atttttatga agcgattcga tgacttgctc
    85501 cccaatggcc acttctttac cttgaaactc tgctggcttg tctgcactgt aaacagcaat
    85561 tggcagctct ccagtatttc catatggatc ccataatgct ttgatgttaa accacgcata
    85621 tagcgaaggg atgatcatga tcgcgatgat cagaagtgtt gcaatcgggt ttttgaaaat
    85681 ccgtttccag tcaagcaaat ataatcgaag cgtatttttg attgactgca tagttctccc
    85741 ccttcttctt ttttcagctg aattacttta ccatgtattt catacttttt tcaatcggct
    85801 tttttcaaaa aagtcgaatt tttggacata tcacggcaat tgtctcattg taatctctta
    85861 catattgaga aataattaag gattacagcc tttactttat catgaaataa aagggaaaga
    85921 taattttatg ccaaagtttt tatctattat acgtatacgc aaaaaagcta aaacattttt
    85981 tgtctttgcc cctacaaaaa tgtttcagct tttttcgttt tttattcctg tatttcttat
    86041 ttttcgaatg acaggatgat tttcccagca ggaggatgtc cttcaactag ctgatgtgct
    86101 tggatgacat gcttaagtga agcagacaga ttcattgaaa taaaaacatg aaaggctcct
    86161 ttttggtagg ctttgattat gccagcaaag gcttctgcat ctagatattc ctttcttgga
    86221 acaaacgatt cataaaagcc ttcctttttt tgccgcaaat ctagatctgg gaggtcattt
    86281 aatgcaacat acctgccgcc tggtttcatg atctggatgc cggtttctcc tttgatactt
    86341 cctttcgtcg catcgattac aaggtctgcc tgatcagcaa attgcagacc tggatttgtt
    86401 ttatcataag cagcaaatgc tgaagctcct aattttttta ccttttcctc atttttactg
    86461 gatgcactgg ttaagattcg gattcccttt tcatggagaa gctggatcag acttgacccg
    86521 accgcacctg atgctcctag tatcattaca gtatctgtcg gctggatttc cgtcaaatgg
    86581 ttgattagat tataagccgt aattcctggc gtgaccattc cggctgcttc ttcccaactc
    86641 attttatctg gaatttttgc tacttttgct gatggcaaca cgacttcttc tccgtaagtc
    86701 ccgccgactg catggacagc tactcgatca cctacatgaa cagttgtgac atctgaacca
    86761 atctctgtca caataccagc gccatcatta cctggtacat aagggaattt caatgttcgt
    86821 atctctttca tttctcctaa acgcaaggca acatcataag gattcacact gaatgctttt
    86881 atttctacac gaacatgtcc agaaccgact tctctgggat gagcatcgat ttcttcaaaa
    86941 acatcttgtg cttctccgta accgttaatt gcaaatcttc gcatggtcac catctccctt
    87001 tttctttaag tttacgttta tgcctttatt ttgacaaaaa aaatgtcttt tcgttttggc
    87061 gcatgattca aaacaaaata gttgtctttt ttcgcgtaac gtgttaaatt atttatctaa
    87121 aagcgtaaag attacgattt acgctaataa aaaataattg gaggaatttg ttgatggcta
    87181 aaaaatcaaa gattgccaaa gcaaaaaaac agcaagaaac gattgaacgc tatgctgctt
    87241 tacgccagga attaaaagcg caaaaagatt acgaaggctt gcgtaaactt cctttagatg
    87301 ctcgtccaga acgattgaaa aatcgtgatt taatcgatgg acgccctcgc ggatacatgc
    87361 gtaaattcgg gatgtcccgt ataaaattcc gtgaattagc acataaaggt ttgattccag
    87421 gagtaaaaaa agccagctgg taatccaagt ctggctgtaa tgtaaaggag gagggcgcat
    87481 ggaccactcc ttggatcatc aaaaaagata cgttcgcttc ttctagcaga aatgctggta
    87541 ctagaagcag acgtatcttt ttttacgcac agctggtcct tttttatatg taaaggctgc
    87601 atggttactt cgctatattt atttagtaac aggagtcagt gcttcgatac ccacactaat
    87661 tttcaatgct cggtcaactt ctgccataat gggtcgatcc aaatggcaga ccttttcttt
    87721 caatcttgat ttatcgatcg ttctgatctg ctccaacagg ataacggagt ctctttctat
    87781 tcctgttcca gtagctttga tcccaatgtg agtcggaagc ttaggtttag ccatttttgc
    87841 cgttatggcc gcaacaatga tcgtcgggct aaaatggtta cctagattat tttgaataac
    87901 caaaactggg cgtgttccgc cttgctccga tcccacgact ggagaaagat cagcaaaata
    87961 aatatctcct ctttttacca taggatcctc cttattcttg atattctctt ggtatccgct
    88021 gtcctaatcc gcaagccact tcatagtgga tcgtacctaa ttgatctgct atatcctgca
    88081 tagtgatttc tttgtctgca tttttgccga taagcgtcac ttttgttccg acaggaaatt
    88141 cttgaggcaa tcggatcatc agttgatcca tacatacacg cccaatcgtt tcacagtagt
    88201 tcccttctac caatagagaa aaaccctgca tctttcgtgg ccagccatct gcataaccga
    88261 tcggtatcgt tccgatccac tctgcttcag gagtgatata tgtttctcca tagccgattc
    88321 cttcacctgc tgggagtttt ttgacttgaa tcaattcaga aaccagctca agtgctggct
    88381 gcaatggata aacttccggt agtgcgtgcc cagagggatt aagtccgtac attgctactc
    88441 cataacgaat catattgccg attgtttcat catgccataa agccgttgca ctattgctga
    88501 catgtacata tcgaggaagt tcgggaagtt ttttcagcac ttctataaag cgttcttttt
    88561 gcagattcca atatgtatca tctgcctgat ctgctgtaga aaaatgggta aataaacctt
    88621 cccattctaa tacacgactt tctttgatga atgcagctgc ctcttttact tcttcaggtg
    88681 tacaaaatcc gatacgaccc atcccagtgt ctgcttttag atgaatcgaa agaggcgtct
    88741 ctagcttact tttctctaaa acagcagctg ctgcttgcaa ccattcccgt gtacctgcag
    88801 ttactgataa atcatgagca acagctacag tcagtgcgtc gtaaggaacc atattcaaga
    88861 taaggatggg ctgagtaaat ccagcttctc tcaactcgac tccttcatct aaatttgaaa
    88921 cacaaaagcc cgaagctcca cctgcaactg ctgcctcagc tgtttcaata gctccatgac
    88981 cgtatccgtt cgcttttact actgcaaaca attcttttcc ttgcggaagt cttgcaactt
    89041 catttgctac attttcagtt atagcttttt tatgtatcac tgctttcgtt gggcggtgcc
    89101 aagcgacaac catcgttttt ccttctttct agtcttccaa aataatttgt gcaaaagctg
    89161 tttcatttgt atgagtaata ctgacccata cattgccttc atgaggtgaa cgcgtcacaa
    89221 caggctgacc attttcattt tttaaaatct cgacatcttg aaaggtaact tttccgatcc
    89281 cagttcccca agctttagag aaagcctctt tgcaagcgta acgcccgcct aaaaattcta
    89341 cttggcgatg aaaaggcaag gactggaata gtttcatttc atcagaggtc aagattcgtg
    89401 caatgaattt tggtttttct tcgatcagcc gagtgatcct aggaagttcg actgcatcga
    89461 tcccaattcc ttttatcata tgcttttctc ttttcaataa atttagaaca ctctcattct
    89521 accaaattat aaccattttg ccacaaataa gtccacaaat aacaaaaatg aaagtttctc
    89581 ttaaaaaaga gaaactttca ttttatgttt aatcgttatt tgaacggatt acaaatccgc
    89641 gttttttatc attgtgacgt ttaccgccat ctttttggct gttattattt ttgctataac
    89701 cgccttttga tttgtttcca cgataattac cgccgtcacg acgattacgg ttgtttccgc
    89761 caccacgttt gccattttta ttgtatcctt ttttctgcat tggcaatggg cgttctggtg
    89821 tgatcttcac tggcactgca tcagctggat cttttgctgt cgtttttagc aaaagtgcaa
    89881 ctaaatcttg tgcagaatat tcttctaata atttatctgc tgtttgcagg tatttatcta
    89941 gaccattttc tgcaagtttt gtttcgattt gttcaactgc agcacctaat tgacctttga
    90001 atgcttcttt ttcggttggt ggacgtaaag tcgtcatccg ttttttcgtc aagttttcaa
    90061 tcacatgcaa gtagtccatt tcatttggtg taacgaaagt cacagacata ccgccttttc
    90121 cggcacggcc tgtacgtccg atacggtgaa cgtagctttc tggatcttga gggatatcat
    90181 agttgtacac atgagtgaca cctgaaatat ctaatccacg tgcagcaacg tctgtcgcta
    90241 caaggatatc taaatgaccg ctcttaaatg aacgcaatac gctcattcgt ttttgttgag
    90301 acaaatcgcc atggattcct tctgcacggt aaccgcgtgc ttctaatccg cgtgccaatt
    90361 cgtctacacg gcgttttgta cgtccgaaga cgattgttaa ttcaggtgtt tgaacatcaa
    90421 acaagcgagt catgatatcg aatttttcgt attcttttgc gcggacatag tattgatcga
    90481 tcaagtcagc tgtcatttct ttcgctttga tcttcacatg atgtggattt ttcatgaatt
    90541 taacgccaat attcttgata gctggcggca tcgttgctga gaaaagtaat gtttgtcttt
    90601 gatcaggaac ttgtgaaatg attttttcga tatcttccaa gaagcccata ttcaacattt
    90661 catctgcttc atccaaaact aatgtttgaa cggttccaag tttcaatgtg tggcggttaa
    90721 tgtggtcaag catacgtcct ggtgtaccca caacgatatg tggacgatct ttcaagccac
    90781 ggatctgacg gccaatatct gctccgccgt aaacggcttg tacacgtact tttttgtcgc
    90841 gccctaaacg atataattct tcttgtgttt ggatagctaa ttcacgtgtt ggtgcgataa
    90901 ctaaaccttg taattcgtga cgatctggat cgattttttc taacattggt aatccaaatg
    90961 cagctgtttt ccctgtacct gtctgtgctt gtccaataac gtcttttcca gctaatgcta
    91021 gtggaatcgt ttctgcttgg ataggcgttg cttcttcaaa accagcgcgt tcgactgatt
    91081 tcaacaattc tggtgataat tctagttctt taaatttcaa atgaatcctc ctatatggtt
    91141 gttaactgag gatgtagctt ttttgctacc ccgtcgattt gttcggtcaa agtgtattgt
    91201 ttctttttga cagtggccac gcacgttttt atccattctt agcccctgtg gcaccaccga
    91261 aaaatcactt tgcgaagttt aggcattaga aaagccaaac gggtcgcgtt cagctttatc
    91321 attattatga catctggctt caacaagttg tttctatggc aaaaccagtc caatttctct
    91381 tcatctttcg gcaataggtt attctaacat agtttttcgt tttctgcaag tttacagatt
    91441 tttatttcat ttgctgaagt tgttctacca cttcaaataa gcccatgctg ttgctgcctt
    91501 ttagcataat actatctgtt gcttcaagat tttgtttgat agcatcaatc aatttattct
    91561 tttctttttc aaaatagaac acaggtagtt gggggtattt ttggttcaat tcttctttta
    91621 gtgcatgcat ttgttcccca taaaggaaaa tcatggcgaa tttctcagga tcgatatgtt
    91681 cactcatttg cgcatgcata tttaaagact ctttccctaa ttccagcata tctgctaaca
    91741 cagcgattct gcgtcctttt gttggcagat ttttaaaagt atctaatacc agtcccatgg
    91801 ctgttggatt tgcattgtag acatcgctca aaatagctgc accatttcct gccgttagcc
    91861 attcggttct gttttgagtg atagaaacat gggcgagccc ttggcgtatc tcaggtatgg
    91921 acagtccaaa gaagcgcccg atcgtatacg caatcaaagc attcgttaca ttataacttc
    91981 caggcagcgg gatcgtaaat gtttctcctt ctactacaaa atcggtttgt gcttttcctt
    92041 ttgctgtcac ttccgcatga acatcgccat ttccaatacc gaatgttgtg actgtttgta
    92101 ctaatgttgc cgttaaaggt gtcagcaacg gttcatctgc gggcacaacg agtaatccgt
    92161 ctgatacaag tccgtcagtg atttccattt tggctttagc gattccttct cgcgacccaa
    92221 gattttctat gtgtgcttca ccgatcattg tgattgctgc agcttttggt tgagccataa
    92281 gtgaaagttc cgtgatttca ttggcgtgat ccatgcccat ttccaaaacg atcttttctg
    92341 tatcttgggg catgtgcaaa atcgtgtaag gaagaccaat attattgttg taatttcctt
    92401 gcgtcttata tgttttgtat ttttcagaca agacagacgc ggttagatct ttcgtggtgg
    92461 ttttaccatt gcttcctgta attgcaacga cttctgcgcc tactttttcc aagtaatagg
    92521 ttgccaattg ctgcatggct ttcaaaggat ctgctacagg aataactgga aaattttttg
    92581 gagcttgttc aacaggcaag ctccaaaatg aagcagatgc tccgtttgtg atcgcttgct
    92641 ggataaattc atgtccatct cgcgtaccag caagaggaac gaataaactt ccttcggtta
    92701 tcttccggct gtcaaattcg acattggtaa tctttgtcag tgcagagcat tcgctgccca
    92761 cggcttgtgc aatttcttgt attgttagat tcattgagat ctctcctaaa aaaggttgtg
    92821 aaaaaagcgt ttggcctaaa cggcgggttc gcatgactct cacttccaag taaatttctc
    92881 tattcgtatt tagaagctag aatgaacgcg gttacgcaat cactccagcc tcttctttat
    92941 ttctctttat tcattttttt caaaaaaaga ttgacgctga tggtatcgat tcattcctaa
    93001 ctggatcagt tcttcaatca aatcaccgta tttcaagccc atattttccc ataagagtgg
    93061 gtacatactg aactccgtaa atcctggcat agagtttaat tcattcagga ataattcatt
    93121 tttatttgtc aaaaagaaat cgcaccggct caatccgctt ccacctaaca tcgtgtaagc
    93181 taacttcgcg tactcttgcg ctttttgata aacttcttct ggcacttcgg ctggaatctg
    93241 catttcgatt ttattattga tatattttgc ttcataatca tagaatgcta cgtcttttac
    93301 gacttcacca ggcaaagtcg tccgaacgtc ttcatttcct aatacagcaa cttcgatttc
    93361 gcgcgcttca attccttgtt caacgattgc tcgagaatca tactgatagg ctgttgctaa
    93421 agcattttgc agctcttctc ggttttctgc ttttgtaatg ccgacactag aacccatatt
    93481 cgccggtttg acaaacatcg gataaagcaa agaaccttca cattgatcaa atactttttt
    93541 aggattttct ttccattgat tcttaagtac tggtacataa ggaacttgcg gcacaccagc
    93601 agcttgtaaa atatacttgg tcatgatttt atccatggca catgcactgg tcaatacgcc
    93661 tgcgccgaca taaggcatat tcaatgtctc taagaagcct tggatcgttc catcttcccc
    93721 gtttggccca tgtaaaactg gaaaaacgat ggctccttct tctttgattt cgcccggatt
    93781 gatcactttt cctgtaaagc cttcctctgt ctgtccactt gggtcccatg aaagatgcaa
    93841 gacatctttg ctggcaggtt tttctgttag taatggacct ttgacccatt gtccttcttt
    93901 tgtaataaat acgagttgaa cttggtaata attataataa atggcattta aaactgaaaa
    93961 tgcggaaaga atggacactt catgctctgc gctgcgtccg ccatatagta aagtaatctt
    94021 caaaagattg tcctcctgta ttctaaatcg ttcagtgatt tactctcata ttttagcaca
    94081 aaacaaaaat aaacgaataa cttatttata aaaaaacgag ccagcttcta aaagaacgaa
    94141 cgtcatttat tttttgtcag ctagattatt tgtcccgttt tttataaaaa gttatgataa
    94201 agaaaaggag ggatgatgat ggcgcaaaca aatccacgca gccgcttggc agaaatcatt
    94261 cgagtgttca tcaattacaa tgtagtgcca aactttgtac agcaaaaaaa tcccgaacag
    94321 gtaaaaaaag cctttgaaga actaggcccc accttcatca agataggaca gatgctttct
    94381 gttcgagaag atttgctttc ttctgccttc actcaaacat tcaaaacatt acaagatagt
    94441 gtacctagcg acacattttc aacggtcaaa aagacgatcg aaacagaact ttctctttca
    94501 ttatcagata tattcgatga tttttcaaaa tcgccttttg cctctgcttc tatggggcag
    94561 gctcatcgtg ccaagctgaa gaacggtgac gcggtagtcg ttaagatcca acaccctaac
    94621 atcgccgaag agatccgact tgatcttcaa ctatttgaac gagcgatccc tttgatcaaa
    94681 tacattcctg aaactagcgt agttgatcta aaaggggtac tccaagaagt aaaacgttcc
    94741 ttgatcaatg aaatggactt cttaaaagaa agtcaaaatg gcgaacaatt ctatcaaaag
    94801 aacaatggct ggaaagagat ccgcagcccc aagatttatg acgctttttg ttcgaaaaaa
    94861 gtgatcgtga tggaagaaat gtctggtaaa aacttgaatc atttaatgaa cgccgaaaac
    94921 aaaactgaaa cttttatcac tggaattcaa aacaagcagc tgaaacaaga agttgcaaag
    94981 cttttagttg agaactttat gaaacaggtg tttgatgatg gttttttcca tgctgatcct
    95041 catccaggga atctgctatt ccatgtacta acaaaagaag aacagacaca agctagccga
    95101 aaaactgaaa cagtccatga aaaagagttc ggttcctttg cttttcgagc ctccacttct
    95161 gcagaagacc ctgttgcgcc atataccatc aactacatcg attttggtat gatggggcat
    95221 ctttctgcag gacttaggca aaaactcaca caagccgttt tagcgctgta tacgaaagat
    95281 gcttatcgaa tcgaaaaagc ggtccttcga ctttgtcagc aagaaggctc gtttgatgaa
    95341 agtcggttcc accaagaact gacaagtttt cttgaacagt attatgattc tcctattgat
    95401 gaaatcaact tgcaagaagt attcacacat gtcgtgacga tttgtcatca aaataatctg
    95461 cagttcgatc gtgatatcac gcttttgctg aaagcatttg ggacattaga aggggtgatc
    95521 cgcgtcctcg atcctgaagt ttccctcatg gaagtcgctt cgccttttgc tcaacattat
    95581 tttctcactc atctggatgt agaagataca ttgaaacaaa gcggattaga cttattggaa
    95641 gggatgaagg ctgcgcctaa gatccctcaa cagctccacc atctattaga aatgtggact
    95701 tcgggacaag gaaaagtcaa tttggaattg aaaaagcaag acaaattatt gagcaggatc
    95761 gaaagtatga tcaatcggct tgttttcggt atgattttag ctgcattgat cgtagggtcc
    95821 tccctgcttg tccaagcagc acctgtagaa aacgcagagg tcgtttcctt attagggata
    95881 tttacgtatg ctattgccgc attcgtcatt atttttttgg caatcgatgc attgatccaa
    95941 atgtataaga agaggaaaaa gtagaaaaag gttgtgacag aaagaatcag cttcaagaaa
    96001 taagaagggg ttttacataa attgtttctc aaatttttgt gaaaattcgg cttatttttc
    96061 gaagaggcta cttctgaaac accgagaata aaaaatgtga aaaagctgtt ctgcacccag
    96121 taataagaac aatcaaaaag ggaacatgga acacatttat cctgtttccc tgttcccttt
    96181 ttgatgtatt cgagcataaa taaaatacct tcgttaccta ctatcttctg tacttggtaa
    96241 accttagtca attgcctttt ttaacgcttg acgaaaagac atatgatctt tctccgtaaa
    96301 gggttttgga ccctttgtcc ttatgccagc ttttcttaac tttccactat gataacgttg
    96361 ttctagaagc gtgccgatat tcgcttcgtc aaattctttt ccagagtgat ggaacactcg
    96421 cacacccttc ggcaatacaa gtgaagctaa accataatct tgcgtgatca aaaagtcatt
    96481 cttcttaacc ataccgacga ttttataatc cgcactatca gctccgcggt ctacgtagac
    96541 gatttggaca tgattagcgt attctttggt cgtataatgg tccacactgg tcacgataac
    96601 cacgtcaaga ccataggatt ctcctaattt tatgacatct tcttttactg gagaaccatc
    96661 tccatcaatg aatagcctca ttctttttcg ccttcttcca taaatgataa acagttacta
    96721 atccgctcag taaaagacca acattgatcc attttgccaa gataagcagt tgtacacttt
    96781 ctatcggcac tgattcatgg ttcagcgtga tcaataatag atggattggc tctcccattt
    96841 cttttgctaa aacaaacaaa agcgtcatca ataaaaactg gtggatcaca ctccctttat
    96901 tttgagtagt atacttgaca acgatccggc gatccattgg aatagctgta tcagtttgat
    96961 aatattcctt atttagccag ctgcctgtta ctacgtgcca gaaactaagc agaaagacac
    97021 ctatcgcaaa accatttgga atcaagagga ccaataaaga agaactcagt ccaagcaatt
    97081 gcatagccaa tggagaccat gttaaacgtt tcaaaatcca tggtccgact atttttgctg
    97141 caatcaagcc tgctgcataa ggcaaataca taaaaatcga aagtttttct aatccgtaca
    97201 gtatgccaac ataaaaactg ctaaataaaa aaaggaaatt ccctagcatt ccatttagaa
    97261 aagtaaacag atttagccag cttggcaact tccattcctt cttctggtgt cctaaaacaa
    97321 acacgaacaa gatgaacagc agcaagaaac cataaatagc aagatcaaaa agcctttcat
    97381 cgaataacaa tcgggcattt ctcaaaagaa acaataagcc aaagaatatc aaaaataaaa
    97441 caagctctcg cgtagctacc agatttcttt tgatgtcttt gaaatccagt tcgtaacgcg
    97501 gataatgact aaccgtatgg taggccatga cgtaaaaaag tgtataaata gtaaagcttg
    97561 ctgcaatctg agtcggtgca ggcaactcaa tcgcttgata caggagaaat agccagatag
    97621 ctgcaaataa gtactttttc ccatccatat tgataaaacc ttgcgtcttt tcgtgaaaat
    97681 tgaccgtggt attagcaggt acaagccatg ctgcactcag ccctagcaaa acacttgaaa
    97741 aataaaataa aggaaaataa aatacgccta atattccaag caatgaacca gcaccaccaa
    97801 tcagcagagc gatcatcagc aacgtgtaac tatctagacc aaatcgtaga ctgttgacta
    97861 aaaatagtcc agtcatccga aaggcataaa acagcgtaaa tggcaaaatc gtcagccaat
    97921 tatgcgagtc tgttagtccc aaaaataaaa gataaggaat aaaaataacc gtgttactta
    97981 agataaaggg cgttgtttgt gtaaaattac gcccaaatcg tttgagtttt tcgaacatga
    98041 caatggctcc ttaaacagtc ttataactaa tctgctcttc gattttagca ttaaagggga
    98101 aataaaccaa ttttacagaa tatattcttg taaaaaaaaa tacagaaaga agcataagct
    98161 tctttctgca tttttacggt acttgattac tcaagcagac aagcccttct ccacaaaagt
    98221 cttgcccgac acccctgcat ccggcaggtg ataaagatag aaatcaatta tcctgtgcct
    98281 ccaaaatcaa ttaggagctc tgtaaaccag agtccttctc gacccgtcgc ataaatttac
    98341 tttaacacag ttcctatgtt tatgcgagag cttttttcat tttataatcg accatttgaa
    98401 atccttgacc gatatctgaa ataagttctt ctgtaatctc aaatccttca tgcttataaa
    98461 cctcgattgc ttgatgattc ccctgattca ctcgcaaaaa gatctctctt tcctttgttt
    98521 gcgcagcaag ttcttcatac cagcgaaaaa ctgaagaagt caatccttga ccacgtactt
    98581 tatttagcaa atataattta cttaaataaa tttttccatt tatctcttca taagcagtgt
    98641 aaccgacagt cttttcttcg ctttttaata aaaagtagga cacaccttct gtcagttctt
    98701 tttgaatctg tttttttgac tgataagttt tcagcatgta atccacttgt ttttgaccaa
    98761 tgatcggcac aaaaacttcc ggccatactt cttgtatgac tgccatcagc tcatccagtt
    98821 cattttctgt tacttttatt ttttccatac acccacgtcc ttttttacat tataaatgac
    98881 cttgcgcttg atggaataaa aagagcatga tcatgctata cacacccacc gaccacggtt
    98941 tcaagaaaat cacaatctgc ataaattccc taaaagataa tttcgtcaaa ccagctacta
    99001 gacagatcaa gtcgtctggc gcaaagggaa gcagcaaggt aacaactagt acttttttaa
    99061 tatctttcgt ttttcttgtc agcaattcat caaatttctg atactttttt ggcgataaaa
    99121 taagctggac aaaggctacg ccatagtaac ggacaagcaa aaatccgatt gcttccccaa
    99181 tcactaggcc aatacaacta tataaaagcc caaatccatt gccaaacatc agcatgcccg
    99241 ctactgagga gatccctccc ggcaaaatag gaatgatcac ttggatcact tgcaacagaa
    99301 caaatatcat cactgcatac ttgccaaatt gctggataaa cgcttgcaaa gacgtgctcg
    99361 atcgaaaaat cccatgatga taagcataaa gaatcaacac aaagaaaaaa aacagaccaa
    99421 taatgggcat gatctgaaga catttttgta aaactgatcc ttttttagac atctatattc
    99481 cccacatctt ttttcattta ttaaaactgc ctccttatga tgtgcacccc aaaagttaga
    99541 ctagaaatct aattaaaagg ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa
    99601 aaagtagttt cagactattt aaataacgaa ggcggctaca aatatcttgc acataaatat
    99661 caaataaatc gtacactggt tagacattgg gtaaggattt ataactatca tggttgggaa
    99721 ggcttggttg gaggtggcaa aagctacact acaaaattta aacttgatgt tatagaatat
    99781 atggaaacaa atggtctttc tatccaagaa actgctaaaa aatttaatat cggttcaaat
    99841 agaactctaa gtaaatggat agagcaatat gaagaaggcg gtgcttcttc acttgagagc
    99901 caaaaaaggg gcagaaaaat tagtatgaat tccaagctaa acattcctaa aaaacttaaa
    99961 gatgagtctc ttgaagaaga agttattcgt ttaagagcag
//
LOCUS       DAMYXM010000003        94390 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.003, whole genome shotgun sequence.
ACCESSION   DAMYXM010000003 DAMYXM010000000
VERSION     DAMYXM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 94390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.003"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            255..686
                     /locus_tag="PNS21_000212"
     CDS             255..686
                     /locus_tag="PNS21_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDI5157958.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            693..926
                     /locus_tag="PNS21_000213"
     CDS             693..926
                     /locus_tag="PNS21_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5157959.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            1350..1550
                     /locus_tag="PNS21_000214"
     CDS             1350..1550
                     /locus_tag="PNS21_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDI5157960.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            1634..2827
                     /locus_tag="PNS21_000215"
     CDS             1634..2827
                     /locus_tag="PNS21_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDI5157961.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            3005..3235
                     /locus_tag="PNS21_000216"
                     /pseudo
     CDS             3005..3235
                     /locus_tag="PNS21_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
     gene            3363..4046
                     /locus_tag="PNS21_000217"
     CDS             3363..4046
                     /locus_tag="PNS21_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDI5157962.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            4355..4428
                     /locus_tag="PNS21_000218"
     tRNA            4355..4428
                     /locus_tag="PNS21_000218"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4389..4391,aa:Arg,seq:tct)
     gene            4587..4946
                     /locus_tag="PNS21_000219"
     CDS             4587..4946
                     /locus_tag="PNS21_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HDI5157963.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            5069..6700
                     /locus_tag="PNS21_000220"
     CDS             5069..6700
                     /locus_tag="PNS21_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HDI5157964.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(6762..7403)
                     /locus_tag="PNS21_000221"
     CDS             complement(6762..7403)
                     /locus_tag="PNS21_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDI5157965.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    7589..7665
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            7685..7993
                     /gene="rplU"
                     /locus_tag="PNS21_000222"
     CDS             7685..7993
                     /gene="rplU"
                     /locus_tag="PNS21_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDI5157966.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            8020..8364
                     /locus_tag="PNS21_000223"
     CDS             8020..8364
                     /locus_tag="PNS21_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDI5157967.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            8377..8670
                     /gene="rpmA"
                     /locus_tag="PNS21_000224"
     CDS             8377..8670
                     /gene="rpmA"
                     /locus_tag="PNS21_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDI5157968.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            9104..11911
                     /locus_tag="PNS21_000225"
     CDS             9104..11911
                     /locus_tag="PNS21_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDI5157969.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            12102..12593
                     /locus_tag="PNS21_000226"
     CDS             12102..12593
                     /locus_tag="PNS21_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDI5157970.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            12696..13685
                     /gene="manX"
                     /locus_tag="PNS21_000227"
     CDS             12696..13685
                     /gene="manX"
                     /locus_tag="PNS21_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDI5157971.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            13718..14521
                     /locus_tag="PNS21_000228"
     CDS             13718..14521
                     /locus_tag="PNS21_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDI5157972.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            14542..15456
                     /locus_tag="PNS21_000229"
     CDS             14542..15456
                     /locus_tag="PNS21_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDI5157973.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFSPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            15539..15913
                     /locus_tag="PNS21_000230"
     CDS             15539..15913
                     /locus_tag="PNS21_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HDI5157974.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            16052..16297
                     /locus_tag="PNS21_000231"
     CDS             16052..16297
                     /locus_tag="PNS21_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDI5157975.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            16469..17533
                     /locus_tag="PNS21_000232"
     CDS             16469..17533
                     /locus_tag="PNS21_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDI5157976.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            17779..18204
                     /locus_tag="PNS21_000233"
     CDS             17779..18204
                     /locus_tag="PNS21_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDI5157977.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            18197..18646
                     /gene="nusB"
                     /locus_tag="PNS21_000234"
     CDS             18197..18646
                     /gene="nusB"
                     /locus_tag="PNS21_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDI5157978.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            18743..19597
                     /locus_tag="PNS21_000235"
     CDS             18743..19597
                     /locus_tag="PNS21_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HDI5157979.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            19598..20935
                     /locus_tag="PNS21_000236"
     CDS             19598..20935
                     /locus_tag="PNS21_000236"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDI5157980.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            20940..21164
                     /locus_tag="PNS21_000237"
     CDS             20940..21164
                     /locus_tag="PNS21_000237"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDI5157981.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            21166..22056
                     /locus_tag="PNS21_000238"
     CDS             21166..22056
                     /locus_tag="PNS21_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDI5157982.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            22065..22883
                     /locus_tag="PNS21_000239"
     CDS             22065..22883
                     /locus_tag="PNS21_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDI5157983.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            22990..23439
                     /locus_tag="PNS21_000240"
     CDS             22990..23439
                     /locus_tag="PNS21_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDI5157984.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            23457..25139
                     /gene="recN"
                     /locus_tag="PNS21_000241"
     CDS             23457..25139
                     /gene="recN"
                     /locus_tag="PNS21_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDI5157985.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(25194..25310)
                     /locus_tag="PNS21_000242"
     CDS             complement(25194..25310)
                     /locus_tag="PNS21_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HDI5157986.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(25383..26339)
                     /locus_tag="PNS21_000243"
     CDS             complement(25383..26339)
                     /locus_tag="PNS21_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDI5157987.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            26510..26878
                     /locus_tag="PNS21_000244"
     CDS             26510..26878
                     /locus_tag="PNS21_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HDI5157988.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            27048..27479
                     /gene="mraZ"
                     /locus_tag="PNS21_000245"
     CDS             27048..27479
                     /gene="mraZ"
                     /locus_tag="PNS21_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDI5157989.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            27500..28459
                     /gene="rsmH"
                     /locus_tag="PNS21_000246"
     CDS             27500..28459
                     /gene="rsmH"
                     /locus_tag="PNS21_000246"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDI5157990.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            28464..28865
                     /gene="ftsL"
                     /locus_tag="PNS21_000247"
     CDS             28464..28865
                     /gene="ftsL"
                     /locus_tag="PNS21_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDI5157991.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            28866..31058
                     /locus_tag="PNS21_000248"
     CDS             28866..31058
                     /locus_tag="PNS21_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDI5157992.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            31084..32046
                     /locus_tag="PNS21_000249"
     CDS             31084..32046
                     /locus_tag="PNS21_000249"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDI5157993.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            32060..33421
                     /locus_tag="PNS21_000250"
     CDS             32060..33421
                     /locus_tag="PNS21_000250"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDI5157994.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            33447..34535
                     /gene="murG"
                     /locus_tag="PNS21_000251"
     CDS             33447..34535
                     /gene="murG"
                     /locus_tag="PNS21_000251"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDI5157995.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            34550..35770
                     /locus_tag="PNS21_000252"
     CDS             34550..35770
                     /locus_tag="PNS21_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDI5157996.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            35926..37251
                     /gene="ftsA"
                     /locus_tag="PNS21_000253"
     CDS             35926..37251
                     /gene="ftsA"
                     /locus_tag="PNS21_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDI5157997.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            37274..38515
                     /gene="ftsZ"
                     /locus_tag="PNS21_000254"
     CDS             37274..38515
                     /gene="ftsZ"
                     /locus_tag="PNS21_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDI5157998.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            38532..39209
                     /locus_tag="PNS21_000255"
     CDS             38532..39209
                     /locus_tag="PNS21_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDI5157999.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            39223..39822
                     /locus_tag="PNS21_000256"
     CDS             39223..39822
                     /locus_tag="PNS21_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDI5158000.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            39837..40124
                     /locus_tag="PNS21_000257"
     CDS             39837..40124
                     /locus_tag="PNS21_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDI5158001.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            40173..40955
                     /locus_tag="PNS21_000258"
     CDS             40173..40955
                     /locus_tag="PNS21_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDI5158002.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            40976..41683
                     /locus_tag="PNS21_000259"
     CDS             40976..41683
                     /locus_tag="PNS21_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDI5158003.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            41945..44743
                     /gene="ileS"
                     /locus_tag="PNS21_000260"
     CDS             41945..44743
                     /gene="ileS"
                     /locus_tag="PNS21_000260"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDI5158004.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(44792..46318)
                     /locus_tag="PNS21_000261"
     CDS             complement(44792..46318)
                     /locus_tag="PNS21_000261"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDI5158005.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(46333..46980)
                     /locus_tag="PNS21_000262"
     CDS             complement(46333..46980)
                     /locus_tag="PNS21_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDI5158006.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(47164..47493)
                     /locus_tag="PNS21_000263"
     CDS             complement(47164..47493)
                     /locus_tag="PNS21_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HDI5158007.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            47670..48398
                     /gene="sfsA"
                     /locus_tag="PNS21_000264"
     CDS             47670..48398
                     /gene="sfsA"
                     /locus_tag="PNS21_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDI5158008.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            48414..49427
                     /locus_tag="PNS21_000265"
     CDS             48414..49427
                     /locus_tag="PNS21_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDI5158009.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            49427..50704
                     /locus_tag="PNS21_000266"
     CDS             49427..50704
                     /locus_tag="PNS21_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDI5158010.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            50767..53469
                     /locus_tag="PNS21_000267"
     CDS             50767..53469
                     /locus_tag="PNS21_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDI5158011.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            53621..53938
                     /locus_tag="PNS21_000268"
     CDS             53621..53938
                     /locus_tag="PNS21_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDI5158012.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            53968..54288
                     /locus_tag="PNS21_000269"
     CDS             53968..54288
                     /locus_tag="PNS21_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDI5158013.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            54396..55856
                     /locus_tag="PNS21_000270"
     CDS             54396..55856
                     /locus_tag="PNS21_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDI5158014.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            55924..56145
                     /locus_tag="PNS21_000271"
     CDS             55924..56145
                     /locus_tag="PNS21_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158015.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            56176..56358
                     /locus_tag="PNS21_000272"
     CDS             56176..56358
                     /locus_tag="PNS21_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HDI5158016.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            56358..56771
                     /locus_tag="PNS21_000273"
     CDS             56358..56771
                     /locus_tag="PNS21_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDI5158017.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            56894..58075
                     /locus_tag="PNS21_000274"
     CDS             56894..58075
                     /locus_tag="PNS21_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5158018.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(58146..58310)
                     /locus_tag="PNS21_000275"
     CDS             complement(58146..58310)
                     /locus_tag="PNS21_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158019.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            58464..58537
                     /locus_tag="PNS21_000276"
     tRNA            58464..58537
                     /locus_tag="PNS21_000276"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58498..58500,aa:Arg,seq:cct)
     gene            complement(58606..59745)
                     /locus_tag="PNS21_000277"
     CDS             complement(58606..59745)
                     /locus_tag="PNS21_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDI5158020.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(60044..60679)
                     /locus_tag="PNS21_000278"
     CDS             complement(60044..60679)
                     /locus_tag="PNS21_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158021.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(60792..61427)
                     /locus_tag="PNS21_000279"
     CDS             complement(60792..61427)
                     /locus_tag="PNS21_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HDI5158022.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(61461..61922)
                     /locus_tag="PNS21_000280"
     CDS             complement(61461..61922)
                     /locus_tag="PNS21_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDI5158023.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(62052..62483)
                     /locus_tag="PNS21_000281"
     CDS             complement(62052..62483)
                     /locus_tag="PNS21_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDI5158024.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(62501..62821)
                     /locus_tag="PNS21_000282"
     CDS             complement(62501..62821)
                     /locus_tag="PNS21_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI5158025.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            63120..63896
                     /locus_tag="PNS21_000283"
     CDS             63120..63896
                     /locus_tag="PNS21_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDI5158026.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            63911..64114
                     /locus_tag="PNS21_000284"
     CDS             63911..64114
                     /locus_tag="PNS21_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5158027.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            64130..64468
                     /locus_tag="PNS21_000285"
     CDS             64130..64468
                     /locus_tag="PNS21_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HDI5158028.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            64455..64634
                     /locus_tag="PNS21_000286"
     CDS             64455..64634
                     /locus_tag="PNS21_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158029.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(64677..65147)
                     /locus_tag="PNS21_000287"
     CDS             complement(64677..65147)
                     /locus_tag="PNS21_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158030.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            65234..65932
                     /locus_tag="PNS21_000288"
     CDS             65234..65932
                     /locus_tag="PNS21_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDI5158031.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            66110..66451
                     /locus_tag="PNS21_000289"
     CDS             66110..66451
                     /locus_tag="PNS21_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158032.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            66444..67115
                     /locus_tag="PNS21_000290"
     CDS             66444..67115
                     /locus_tag="PNS21_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDI5158033.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            67121..67807
                     /locus_tag="PNS21_000291"
     CDS             67121..67807
                     /locus_tag="PNS21_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158034.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            67810..68559
                     /locus_tag="PNS21_000292"
     CDS             67810..68559
                     /locus_tag="PNS21_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5158035.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            68571..68840
                     /locus_tag="PNS21_000293"
     CDS             68571..68840
                     /locus_tag="PNS21_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158036.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            68845..69009
                     /locus_tag="PNS21_000294"
     CDS             68845..69009
                     /locus_tag="PNS21_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158037.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            69002..69304
                     /locus_tag="PNS21_000295"
     CDS             69002..69304
                     /locus_tag="PNS21_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158038.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            69301..69462
                     /locus_tag="PNS21_000296"
     CDS             69301..69462
                     /locus_tag="PNS21_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDI5158039.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            69459..69764
                     /locus_tag="PNS21_000297"
     CDS             69459..69764
                     /locus_tag="PNS21_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158040.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            69764..70120
                     /locus_tag="PNS21_000298"
     CDS             69764..70120
                     /locus_tag="PNS21_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HDI5158041.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            70107..70325
                     /locus_tag="PNS21_000299"
     CDS             70107..70325
                     /locus_tag="PNS21_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158042.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            70322..70741
                     /locus_tag="PNS21_000300"
     CDS             70322..70741
                     /locus_tag="PNS21_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HDI5158043.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            70738..71295
                     /locus_tag="PNS21_000301"
     CDS             70738..71295
                     /locus_tag="PNS21_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HDI5158044.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            71292..71588
                     /locus_tag="PNS21_000302"
     CDS             71292..71588
                     /locus_tag="PNS21_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158045.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            71665..72078
                     /locus_tag="PNS21_000303"
     CDS             71665..72078
                     /locus_tag="PNS21_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDI5158046.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            72387..72539
                     /locus_tag="PNS21_000304"
     CDS             72387..72539
                     /locus_tag="PNS21_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158047.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(72536..72811)
                     /locus_tag="PNS21_000305"
     CDS             complement(72536..72811)
                     /locus_tag="PNS21_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158048.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            73265..73471
                     /locus_tag="PNS21_000306"
     CDS             73265..73471
                     /locus_tag="PNS21_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158049.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            73667..73834
                     /locus_tag="PNS21_000307"
     CDS             73667..73834
                     /locus_tag="PNS21_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDI5158050.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            73860..74204
                     /locus_tag="PNS21_000308"
     CDS             73860..74204
                     /locus_tag="PNS21_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDI5158051.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            74209..74490
                     /locus_tag="PNS21_000309"
     CDS             74209..74490
                     /locus_tag="PNS21_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158052.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            74593..74907
                     /locus_tag="PNS21_000310"
     CDS             74593..74907
                     /locus_tag="PNS21_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDI5158053.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            74885..76579
                     /locus_tag="PNS21_000311"
     CDS             74885..76579
                     /locus_tag="PNS21_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDI5158054.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            76599..77777
                     /locus_tag="PNS21_000312"
     CDS             76599..77777
                     /locus_tag="PNS21_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDI5158055.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            77740..78426
                     /locus_tag="PNS21_000313"
     CDS             77740..78426
                     /locus_tag="PNS21_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDI5158056.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            78426..79586
                     /locus_tag="PNS21_000314"
     CDS             78426..79586
                     /locus_tag="PNS21_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDI5158057.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            79596..80471
                     /locus_tag="PNS21_000315"
     CDS             79596..80471
                     /locus_tag="PNS21_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158058.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            80468..80779
                     /locus_tag="PNS21_000316"
     CDS             80468..80779
                     /locus_tag="PNS21_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158059.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            80769..81122
                     /locus_tag="PNS21_000317"
     CDS             80769..81122
                     /locus_tag="PNS21_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDI5158060.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            81112..81513
                     /locus_tag="PNS21_000318"
     CDS             81112..81513
                     /locus_tag="PNS21_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158061.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            81506..81910
                     /locus_tag="PNS21_000319"
     CDS             81506..81910
                     /locus_tag="PNS21_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158062.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            81922..82527
                     /locus_tag="PNS21_000320"
     CDS             81922..82527
                     /locus_tag="PNS21_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI5158063.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            82547..82909
                     /locus_tag="PNS21_000321"
     CDS             82547..82909
                     /locus_tag="PNS21_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158064.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            82912..83094
                     /locus_tag="PNS21_000322"
     CDS             82912..83094
                     /locus_tag="PNS21_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158065.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            83111..86542
                     /locus_tag="PNS21_000323"
     CDS             83111..86542
                     /locus_tag="PNS21_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDI5158066.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            86593..87330
                     /locus_tag="PNS21_000324"
     CDS             86593..87330
                     /locus_tag="PNS21_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI5158067.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            87340..89631
                     /locus_tag="PNS21_000325"
     CDS             87340..89631
                     /locus_tag="PNS21_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI5158068.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            89655..91781
                     /locus_tag="PNS21_000326"
     CDS             89655..91781
                     /locus_tag="PNS21_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158069.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            91798..91947
                     /locus_tag="PNS21_000327"
     CDS             91798..91947
                     /locus_tag="PNS21_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158070.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            91944..92390
                     /locus_tag="PNS21_000328"
     CDS             91944..92390
                     /locus_tag="PNS21_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158071.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            92392..92529
                     /locus_tag="PNS21_000329"
     CDS             92392..92529
                     /locus_tag="PNS21_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDI5158072.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            92567..92860
                     /locus_tag="PNS21_000330"
     CDS             92567..92860
                     /locus_tag="PNS21_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158073.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            92857..93081
                     /locus_tag="PNS21_000331"
     CDS             92857..93081
                     /locus_tag="PNS21_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDI5158074.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            93078..94103
                     /locus_tag="PNS21_000332"
     CDS             93078..94103
                     /locus_tag="PNS21_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDI5158075.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            94293..94366
                     /locus_tag="PNS21_000333"
     tRNA            94293..94366
                     /locus_tag="PNS21_000333"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:94327..94329,aa:Ile,seq:cat)
BASE COUNT        31732 a        15625 c        20027 g        27006 t
ORIGIN      
        1 cacagggcta ttccggttat gagtatgtac tataccatgt aaatcagcag catagcaaag
       61 cattttctcg cccggtaggt ttgcgacctg ccgggcgatt tttgtcgtct tttgcggccc
      121 ggctgtcccc aaaaaaattt tttgagaaat tttcaaaaag gaggtattta gcgggtagct
      181 ggcggccttg tcaaagtctt gttagcggaa agggagagaa ccacctaagt cccccacaga
      241 aagggggtga gaagatggat gctatccccc gcgactatga agcgcggtgc atgttcgatg
      301 ctttttgcaa gacggtactg cgtaacgagg ccaaaagcta cttggctgaa atgaagcgcc
      361 gccgtgaccg tgaagtgtca ctaagctctt tgtcacaggc agatttggac aagctctgta
      421 cagtagatca ctaccccagc gacagcttta cgttttcgtc gcatggctat gacctacata
      481 tcaacaatga gcttgttgcc gaagcctttg ccgcgctgcc cagcatggag cagagtattt
      541 taatcctgca ctgtgttctg gatatggcag atggtgagat cggcggcctt gtgggaatgt
      601 cccggagtgc cgttcagcgg cacaggacaa ataccttatc ggagctgcgg aaacagctaa
      661 aggcgttgat gccgaaggga ggttagacgc ggatgaagaa cccgaactac aagggcattg
      721 agcttttgcc cctttcggtg atcgaggccg cccgcgctgg ggatgccgga gccgtagagc
      781 gcgttttgca gtattacagc ggttacatga acaagctgtg tacccggacg ctctatgacg
      841 aaaacggcta tccgcatgtg tgtctggacg aggccatgaa gcgccgtctg gaaatcaagc
      901 tcatccacgc tatcgtcacg aactgacgaa ccggccccgg cagcatgaaa cggcttaccc
      961 tttccccgtt tcggtgtttt cccggtggcc tgatcgcacc ttgataaata tgcccgcaag
     1021 ataacggcgg cgcatcatag ggcatacgga cacattctgt ctgtgccgag ccggacggct
     1081 ggtgcgccat gatacttttc cctacgggaa aagcggagcg aggaacacca cgccgcaatg
     1141 tgagcgtggc ggcttgcggg cgacgacgta caggcaggac tatgatactc ccttatagcc
     1201 acagtccgag cgttaaaagc gtcgcaggca atgggtaggg ccgcgtgaga accacgcggg
     1261 ggtgaaagtc ccgtggagct gtgccagcag ccgtccgtct atgcccctaa atcacacaaa
     1321 cctttgaatt tgtgaaaggg ggctgcatta tgaaccatga tgtgcctatt tgggaaaaat
     1381 acacgctgac gattgaggaa gcgtctaaat atttccgtat cggagaaaac aaattgcgta
     1441 agctggccga ggaaaatccg gcctctggct gggtcattat gaatggcaac cgcatccaga
     1501 tcaaacgcaa gcagtttgag aaaataattg acactttgga cacaatctaa tagtgaaaaa
     1561 gggccgcaga tatgttataa taatttataa gcatatatgc ggctctttcc gtcatggaaa
     1621 ggagcatgac gagatgtcag aaaaaagacg agacagcaaa aatcgcgttt tgcggtctgg
     1681 agagagccag agaaaagacg gacgatatgc ttacaaatat gttgatacct ttggaaaacc
     1741 gcagtttgtt tactcatgga agttagtgcc tacggataag accccggcag gtaaacggga
     1801 ggatatttca ctccgggaaa aggagaaaga aatccagaaa gaccttgacg acgggataga
     1861 caccatcggc aagaaaatga ccgtctgcca gctctacgcc aagcagatca ggcaccgggg
     1921 caatgtgaag cacaacacca aactgggccg ggaacggctg atgcgtattc tggaacaaga
     1981 tcggctgggg agctgcccga ttgacagtgt gaagctgtcc gacgcaaagg aatgggcgct
     2041 tcggatgaaa gaaaagggcc tttcttataa gaccatcaat aacgacaagc gttccctgaa
     2101 agcggctttt tacaccgcca tacaggacga ttgcatccga aaaaacccgt ttgacttcca
     2161 gcttagtgac gttctggacg acgatacgga accaaaggtg cctcttacac cagcacagga
     2221 agaaagtttt ctgtccttta ttcaaggtga taaggtttac caaaagcatt atgacgcaat
     2281 cgttattctg ctggggacag ggcttcgcat ttcggaactg tgcggactga cggacaaaga
     2341 tttggatttt gaaaaccggg taatcatcgt gagccaccag cttttgagaa ataccggcgt
     2401 cggttactat attgacgaac ccaagaccca aagcggcgtc agaaaaatcc ccatgaatga
     2461 ggaagtgtat caggcgttcc agcgagtgat aaaaaaccgc aaaggtgcaa agccgttcat
     2521 tattgacggg tatgccaatt tcctgtttct caaacagaac ggctatccca tgacggcggt
     2581 agattatggc gggatgtttg gcaggcttgt gaagaagtac aacaagagcc atgaagaagc
     2641 gttgccgaaa acgacgacgc cccatgctat gcgtcataca ttctgcaccc ggttagccaa
     2701 tgcgggaatg aaccccaaag ccttgcagta cattatggga cattccaata tcaccatgac
     2761 gctgaactat tatgcacacg ccacattcga ttccgcaagg gcagaaatgg aacggctggc
     2821 agcctgacac aaggccggtt ttactactcc atttactact tttgaggggg aaatggtgag
     2881 aggtaataag aacatttgta agtatctaac aatatcaaaa atgccggaaa agcccggaaa
     2941 cacgggctat accgacattc aagaacttct aaaaagataa tctaaaatcc catataattt
     3001 ttcctcaaaa gctgatacaa agacttatat tgagcctgga gtagtaaaaa agagaaatat
     3061 tatcggtagt cccatctttc agattccata tattggacta gtcctttcaa aaacggcatt
     3121 tgcatggatt caggggttat tctttacgat tgctttttgc ctaacaggaa tcacagcatg
     3181 gaataccttt gtagagttga aaaagaagcg gagacgaaca cagaaatttt tgtaagaatg
     3241 ttaaaaacat aaatgtattc cagagatcca acgactcttc tgaacatgaa acacgagaaa
     3301 acactcatgc agcttgtaga tttagtcagt tgttcgtata atgtgtgaag aggaggagta
     3361 gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga agaactattg gattttatgc
     3421 gtacccagca aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa gctcatgtag
     3481 cagaagtacc agttattccc catgaaacag ttgtattttt gaaattttta cttggacagc
     3541 ttcagcctga acgaatcttg gagatcggag cagctatcgg tttttcatca agtgtcatgg
     3601 ccacgacaat cggagaaaac gctcatgtta cgacaattga tcgtttcgat gtcatgatcg
     3661 agaaagctaa aaaaaactat gaacgtctag gactgacaga taaagtgact ttgcttgaag
     3721 gtcaagcagc agatatcttg ccggagttgt ctggtccgta tgactttatt tttatggata
     3781 gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt acgtttgtta agaaaaggtg
     3841 gcgttttgat ggtagatgac atcttccaag gaggaacgat cttgctccca gatgaagaga
     3901 ttccccgtgg caaacgagct atccatcgta aattaaatga atttcttcgt gttgtgatgg
     3961 atcatcccga tttgacatcg acaatcttgc cattaggtga tggtgtgatc ttaatgacaa
     4021 aggaatcaga aacaatcgag ttgtgataca tgattatggc agcctgtaga taatcgattg
     4081 aatcgagggc tcaattcgaa cttgatgtaa aagtttctat atttgtaagt caattaaagg
     4141 atacaggaaa tctaaataaa caggagtagc atgaagtcaa tgtagctcct gttttcaatt
     4201 gataacctta gaacagctga aggagaatgt ctcgttttaa aagattgatt tagtagttgg
     4261 gccaaaataa ttcattattt gctaattaaa taactatttc gtattgacag aagcaattta
     4321 attatctata attattgatg ttccgctgat tcttgtcata gtagctcagc tggatagagc
     4381 atccgccttc taagcggacg gtcgggggtt cgaatccctc ctgtgacgct aaaaaaccgt
     4441 tagaaactga ggtttctaac ggtttttctt tgttttttgt gccaatattg tttgttttca
     4501 aaggcaaata aaataaaaaa acatccttat cgagtacaat tataaggagc aaaaaaataa
     4561 aactcatcag aaaagaggaa gaagatatga cgatactatc tagaatttta gtgactctag
     4621 ttgcaattga atttttttat atcatgtata tcgaaacggt gagaactgat tctgacacga
     4681 caagtagggt ttttaaaatg agtaaagaag agttaagcag aaaatctgtt caaacattgt
     4741 ttaagaacca aggagtgtac aatggactat taggagtggg actactttat ggagcttatc
     4801 tttcttccgc atctaaagaa atcacaagta tgctattaat atctattttt tttgttgcat
     4861 tatatggcag tttgactagc gatcgttgga ttttactcaa acaaggtggg ctagctctgt
     4921 tggcattgct ttctattttc ttataaatat agaaaaaata agaatttatc taacaggaaa
     4981 ctttgtgaaa aatattactt tatctcatga aatatgatat tctaatgaat ggtattttag
     5041 aaaatttcat gaaaaaggag ttagataggt gttcaaaaat tatgtttcct cactaaaaaa
     5101 agaattttca ggttatgatc tttcaagatt tcagaaagat cttttggctg ggatcacagt
     5161 agctgctgta gcattgcctt tagccttggc ttttggggta tcaagcggag ctgatgcggc
     5221 cgcaggtttg attacggcta tcattgccgg tatcgttatt ggcggtttat caggcggttt
     5281 ttatcaaata tcaggtccga caggtgcgat ggcagctatc ttgatgtcta ttgcggcaac
     5341 aaagggaatg caaggtgtat tgcttgctac ttttttagcg ggtctatttt tacttgcagc
     5401 aggacttttc cgattaggaa cactgacttc ttttatccca gcacccgtaa ttacggggtt
     5461 tacttcaggt attgctatca ttattgcatt aggtcagatc gataatttat ttggtgttca
     5521 ctcggaaggt acaaacgtta ttgaaaaact aagtagttat caaacgatag gatttccgat
     5581 aaacacagcc tctttgatca tgggagggct agtcatttta gggatgttta tctatccaaa
     5641 aaaatgggcg aaacgaatgc ctagttcgct gctggctatc attttggcaa cagcagttat
     5701 gctattgttc catttaccag tagcaactgt gggtaagatt ccacaaacat tgatcagtag
     5761 taatcgtttg aatatgggag attttagctt ttctgccttg caacaagtgg cagttccagc
     5821 tattagcatt gctttattag gaatgattga aagcttgcta tgtggtgctt cggctggccg
     5881 aatggcaaat aagcccttag atagcaatca agaattaatg gcgcaaggaa ttggaaatct
     5941 gttgcttcct ttctttggcg ggattcctgc tactgcggcc atcgcccgta caagcgtggc
     6001 catcaaatct ggtgcacaaa ctagattagc tggtatgata catgcagttg tcttactgct
     6061 ctccatgctt gtttttgcgc cagtgatgtc acagattcca atgccagcac ttgcaggtgt
     6121 tttgattgtg acggcttggc gtatgaatga atgggaaacg atcaaggagc tatttactaa
     6181 taaatattgg tcagcagttc tattgtttat ccttacgatg atttgtacag ttatctttga
     6241 tttaagtatt gctatcgtga tcggggtgat cggcggctgt gtattttttg ttgtcaaaag
     6301 cgctgctatc acgatttcat tagaaacgat cgattggcaa agaatggggc tgccagaaac
     6361 ggaaaagctg aataactgga cagtagtgta tattagtggt ccactgtttt ttatgtcttc
     6421 agaaaaactc aaaaataaat tggaagaact aaatcatcac gatggtgtca ttttctccat
     6481 gcgaggcgta ccaagtattg atttgacggc acgtgcagtt tttgatgaat ttcaagaaaa
     6541 agcagagcaa agaggacaaa cgattatcta tacttccttg cagccagaag tagaaaaaca
     6601 actttctcac ttgtgggaac atacattcac gcatcaacat caaacagttg ctcatgcgtt
     6661 gacagcttta catgcgaaat atactgcaat tagccattaa aacaatgaaa aagaacagac
     6721 aagactgtaa actgaagatt ttatggtctt gtctgttctt tttaatattg ctgtaaaagt
     6781 gaagctagta cttgccagtc ttgatggata acaggtacaa gggagcgatt gtagaaaata
     6841 gctgctggat gaaaaagtgg aagaattcga tagttttttt ctgaccattc gtaggcatcc
     6901 ttttgttctg ttaattgtaa gacaggccct gtatagagtt gaccatgatt tttggtaacc
     6961 gtatattctt tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc aattaatttt
     7021 ggctggacct tcatcagttc gtaatccaat attggtgcat gcgcaaatac ttcggaacga
     7081 gtaggtgtcc gatttggata aacagtttct gactgttgtg tccgtttatt gatccgatga
     7141 gtcacgcgat acgggcggct ccttacagca ctggtgatat acacctcttc tcgtgttaag
     7201 ccagttgaag aaagtgcctg catcaattct tttccagctt gaccgctaaa aggaacatgc
     7261 gtatcaatct ctgttttccc tggggcttct cccacgagca tcagtttagg atgaagaggt
     7321 ccttgcccag cgataaatcc ttctacagga cggccgttca ttcttaaaga agcttcttgt
     7381 atcagttctt ttgggtattc catttatgct cctccttttt cttttattat aagaataaaa
     7441 gaaacacaat gcgactattt tatttggtag aactgtgagg atagaaaagc agaagtattg
     7501 cttgaatttt tgttttactt tttgatttta aatttagttg actaacggtt gctgttctgg
     7561 taagctagta ctgttgtaat tgtggactcc acagctacaa ccgctcagaa caagtaataa
     7621 gcacagcata tgctgtcact tcggatggcg agtctaagta tatcagaagg aggtgcttta
     7681 tagcatgtac gcaattatta aaactggtgg taaacaagta aaagttgaag tgggacaagc
     7741 aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg acgaagttat
     7801 cttagtaggc ggagaatcta caaaagtagg cgctccaact gttgcaggtg caactgttga
     7861 aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat acaaacctaa
     7921 aaaacattca caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg ttatcgacgc
     7981 gatcaacgct taatagcgtt tgagaaagaa ggcctatata tgatcaaagg aacatttaag
     8041 cgaaatgacg caggtcagat cgtttcattc actttgaccg ggcatgctga tgctggtcct
     8101 tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac tgtcaacggg
     8161 attgcctcat tagccggttt tgagccgatt gttgaaatga atgaagaaga aggcggttat
     8221 ctttataccg aagtcacttc aggaatgaca caagaacaaa acaatattgc tgagattcta
     8281 ttagaaaatc ttttattagg attacaatcg atagaagctg aaaactcaga atatatccaa
     8341 attaaaacaa ttaatgataa ataggaggtg cagaccatgt tgttatctat gaatctacaa
     8401 ttatttgccc acaaaaaagg tggcggttct acatctaacg gccgtgactc agaatcaaaa
     8461 cgtctaggtg ctaaacgtgc tgatggacaa acagttacag gtggatcaat tctttaccgt
     8521 caacgcggta caaaaatcta tccaggtgta aacgtgggta tcggtggaga cgacacttta
     8581 tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa aaaacaagtg
     8641 tctgtttatc cagtagctca agaagcttaa ttgaatctta tgattaaaga ggttgtgaca
     8701 agacttttgt cacagccttt tttgtttata aaaataaaag ttgtagtgga ggctacgaag
     8761 tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat
     8821 aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat aattatatgg
     8881 taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt ttcgttcttt
     8941 taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag gtaaatagtt
     9001 gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa tagtctatag
     9061 aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg tcaaagaaaa
     9121 aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg aaattgctga
     9181 agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag tgcgcgaagg
     9241 aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac tcgttacgca
     9301 cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct ctgaagaaaa
     9361 aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag ctaacggcag
     9421 catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa gaggattaaa
     9481 ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg cgatgtatca
     9541 ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt tcaattgtgc
     9601 tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg caaaaggtgc
     9661 ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg gcggtatgct
     9721 gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct tttactttat
     9781 ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag caaatgtacg
     9841 gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt ttgttcggcg
     9901 tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg agaaagtcga
     9961 tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga tttctttgac
    10021 agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag gacaattgaa
    10081 gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat cacccgaaat
    10141 ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc aattagcaag
    10201 caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa ctgtagcacc
    10261 aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata atctatatga
    10321 tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag aagcaatcaa
    10381 tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag accatggttt
    10441 ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca tagaagtgac
    10501 caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc aaaactttat
    10561 ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag gagaagaacg
    10621 gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat atgctgttgc
    10681 gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag aaagtgaaga
    10741 ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg gcagaatcgg
    10801 tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag ttgtccaaca
    10861 gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg atccgaaaac
    10921 agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg gggtcatact
    10981 cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag acactggaat
    11041 tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg tacgaaaggt
    11101 ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt ttcgaggtta
    11161 cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata agaaaaaagc
    11221 tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag aattgatcga
    11281 acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt cgattgtgga
    11341 agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta ctactgggat
    11401 tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg atcaaaatat
    11461 cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg aattcatctc
    11521 cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata acttcatctt
    11581 tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa tctctcagac
    11641 gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc acacagcagg
    11701 aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg aattgacaaa
    11761 tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt tggaagatca
    11821 gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag tccataatca
    11881 gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac ttttatagtg
    11941 tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc gtaaaaaagt
    12001 tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca cagaaatgat
    12061 aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt cgattggcac
    12121 gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag caaacaggtg
    12181 ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga aagtttctac
    12241 taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag aaaatgattc
    12301 aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc accaatcccc
    12361 aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat atcggcggta
    12421 tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat caacaagata
    12481 ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga aaagtacctg
    12541 ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg tagcaagaat
    12601 ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta atatttttga
    12661 atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc ctagcaagtc
    12721 atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc gaacaagaaa
    12781 acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga gccaaattgg
    12841 aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt gatttatggg
    12901 gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat aaatgggcaa
    12961 tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc ttttcaatgg
    13021 aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt gtaaaagtaa
    13081 aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac gcacaaccta
    13141 aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat gtattagcac
    13201 gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa gcagtgcaac
    13261 caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt aagagactaa
    13321 tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc aaaatgatcg
    13381 aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt gaaaatcctg
    13441 aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat gttgggtcaa
    13501 tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga caagaagacg
    13561 ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt aaagtaccta
    13621 atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa ttagcaaaag
    13681 cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt caataatttt
    13741 agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat ttcaatttca
    13801 tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg aagctggtat
    13861 cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg gtgccgctgt
    13921 agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt taggtggaca
    13981 aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg ttgcagggtt
    14041 gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga tggatgcggc
    14101 tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg tatgtttaca
    14161 aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac aaactgtgca
    14221 atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg gtgggggaat
    14281 ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag aagtatggcc
    14341 attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga ttgctctagg
    14401 tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg gtggttcatc
    14461 aaataatgga ggcggaagca actctggtga ccctttaggc gacattttga atgactatta
    14521 atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa aaaagatcgt
    14581 ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga acgtatgcaa
    14641 aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa aacaaaagaa
    14701 gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc atacattgct
    14761 tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg cgcgcctgtt
    14821 gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc tggtgttggt
    14881 gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc ttcattagct
    14941 atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat tattcgttgg
    15001 gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat tactgatgac
    15061 ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg gatgttcgta
    15121 ttagctgccc ttgtacaaag atgggtatca atcaaattct caccagttgt atcaactgta
    15181 aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg tgaaggaatc
    15241 aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa ggtaacaaca
    15301 ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt aacattgtta
    15361 tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg tttattcgta
    15421 gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata gctcgggcgt
    15481 tgtcccgagc tttttttata tagtatactg ttcataaaca aatatctgga gggagaaaat
    15541 ggttcaatca atcaatacaa aagtagactt ggtaattgat gctacttcgt ttacgggtct
    15601 gacagattac gggcaaatca tgataggtga taatggtttt gagttttacc atgcgcgaga
    15661 tagacgcaaa tatatccaaa ttccatggga agaagtggat tatgtactag catcagtcat
    15721 gctaaaggga aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg gtacatacac
    15781 attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg tagatgaaga
    15841 tagaatggta caatcattga gtttctttga tgtagtgaaa cgtggtattc gtagtatgtt
    15901 taaacgcaag taaaaaataa agaaaacttc taagaggagg ttttctttat ttttttactt
    15961 tgcagaagaa aatatgaaat agcaaaacca attatttgtc ccctttaaat cccatatgct
    16021 aaaattgatt agcaaagaag gaggttatat tatgagttgg ttatggacat tgattgttgg
    16081 agccgtgatc ggagcaattg caggtgcgat caccagtaaa ggaaattcga tgggctgtgt
    16141 aacgaatatc attgctggac tagtcggttc aagtattgga caagcgattt tcggcgaatg
    16201 gggaccaaaa gctgcaggta tggcacttat tccatctatt ttaggcgcag tgattttggt
    16261 agcagttgtc tcatttttct ttggaaaacg atcataagtt tttacaaaat agtaaactaa
    16321 aaggagttgc agcgagactg tgactccttt ttggctttat ttatttaaaa taaaaagaag
    16381 ttttccggaa atttttattt aactgtcgtg tacgaagaaa agtttgctat aatggaaaaa
    16441 agcgtgcaaa gaaagaggga gtttcattat gatgctacgt gtagaaaaat tgcgcaaaaa
    16501 aatgcaagaa gaaaatttgg attcattttt ggttaccagt ccgtacaatc ttcgttactt
    16561 aacgaacttt acaggtacga caggattagc tgttattact ttggaaaaag cattttttat
    16621 tactgatttt cgttataccg agcaggcggc agcgcaggct caagggtttg aaattattaa
    16681 aaatgtcgga cctatttttg atgaggtagc aaacttagta cgcaaagaag aactgcactc
    16741 attagctttt gaggaaacaa cagtctcatt tttagaatat tcggttttag aagaaatcat
    16801 cgatgcggaa ttagtacctg tttcacagat gatcgaagaa ctcagagaag tgaaagatga
    16861 agaagaaatc gctattatcg aaaaagcatg tcatattgcg gatatggcat atgatcacat
    16921 cctgaaaatg atccaaccgg gcatgactga gattgaagtc gcaaatcaac tagactttta
    16981 tatgcgttct cttggcgctt caggtgtttc ttttgatacg atcgttgcta gcggattgcg
    17041 ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg acttgatcac
    17101 attggacttt ggttgctatt acgaagggta cgtttccgat atgacacgta cattcgctat
    17161 tggtgatcct ggggaaaaat taaaagaaat ttatcaaata gtcttagatg ctcaattagc
    17221 agttattgat gctgcaaaac caggaatgac tggtgtccaa ttagatgcag ttgctagaga
    17281 ttatatttca aaacatggtt acggggaagc atttggacat tctacaggac atgggatcgg
    17341 tttagagatc cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat ttgtaccagg
    17401 aaatgtgatc actgatgaac cagggatcta cttaccagga atcggtggag tacgtattga
    17461 ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg actcgttcac caaaagaatt
    17521 gatcatctta tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt tcacagactt
    17581 tgttatagaa aatcactcga tttaatgata cactatagaa gaacttttaa gctgtaagag
    17641 ataataaagt tgggtgtttt ctccaattca tagcagtttg cagctgatcg gcggcagttt
    17701 tcttaccacg tgatcactat gccgcatcat agagaattcg gatgtatctc gtgcttaggc
    17761 aaagggagga ataaataaat ggcagatgaa aaaaatttga ttttaaacgc aaaccaagat
    17821 ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat tgcagcttct
    17881 aaagtagaag gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac tgaattatta
    17941 ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg cttgaaagta
    18001 gatctttact gttatctaga atatggtgtt tcagttccta aagtagcaat ggaaatgcag
    18061 gaacgggtca gccagcaagt ccttttcatg acggatatcg atttatctga agtcaatatc
    18121 catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga actttttgaa
    18181 gacgaagagg aagagaatga ataaagaatt atcaagacac gagattcggg aaatggcatt
    18241 gcaggcttta tttccattag acttcaatgc agacctaaca aaagaagatg cgattttcaa
    18301 cgcaatcgaa ttagaccacc gcgacatgat caacgaagat gaatcagagt ttgttcctgt
    18361 ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg ataaagtgat
    18421 tgaaaaacat ttaaaaaaca attggcacat caatcggatc tcaaaaatgg atttggtgat
    18481 cttgcgtatc gcgatttttg aaatgactta tgtgacagat gtaccagcgg ctgttgcttt
    18541 aaatgaagca atcgagttgg caaaaacatt tagtgatgac cgttcacgta aattcgtcaa
    18601 cggtgtttta tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc tagctttttt
    18661 ctatgaatca tcataaacct gttataaatc gggaaaattc tatggtagaa tgaaaacaca
    18721 aaaaatatct gggagtggtt agatggcaga attgattaac ggaaaagaat tagctgaaaa
    18781 aatgcaggca gagattgctg aaaaaatcca aaaattgaaa gataatggta tccatcccgg
    18841 attagtcgtt ttattggtag gggaaaatcc agccagccaa atctatgtaa gaaataaaga
    18901 acgtgtagca aaagaaatcg gaatccattc attggtagag cgttatcctg agacgatttc
    18961 tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa gatcctagtt ttcatggtat
    19021 attggtccaa ttaccattgc caaagcatat agatgaagaa aagatcctgt tagctatccg
    19081 ccctgaaaaa gacgtagacg gttttcatcc gatgaattta ggcagactcc ttgcgggtaa
    19141 accggacaag attccttgta ctccatacgg catcatgaaa atgtttgaag cttattctat
    19201 cgatccggcg ggtaaacgag cagtagttat cggacgaagc aatatcgtcg gtaaaccgat
    19261 ggctcagcta ctcttgatgg cagatgcaac tgttactgtt gctcattcta aaaccgaaaa
    19321 tctggctgaa ctggcacaag aagcagatat tttagtcgta gcaataggac gtggacattt
    19381 tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga tgaatcgtga
    19441 tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa gtggagccta ttgcaggctg
    19501 gatcactcct gtgccaaagg gtgtagggcc aatgactatc acgatgctga tgtatcaaac
    19561 cgtaaaatct gccgaagctt ttggagcagg tgaatagatg gcacaagaat atttaactgt
    19621 cactacatta acgaagtatt tgaaaagaaa atttgatgcc gatccttatt tagaacgcgt
    19681 atatttaaca ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc aatatttcag
    19741 cttaaaagat gaccatgcaa aaatatctgc tattatgttc aaaggagcat tccaaaaatt
    19801 acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt cattatatga
    19861 agcgggaggt tcttatcaaa tctatgtaga gcacatggag cctgatggaa taggtgcttt
    19921 atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt ttcaagggcc
    19981 taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc caagcggagc
    20041 ggttatccgc gatatcatca cgacggtgaa aagaagatat ccaattgtcc aattggtttt
    20101 gttcccaaca gttgtacaag gaaaccaagc agcggacaat gttgtcagga atatccagag
    20161 agtagaagag atgggcaatt tcgatacaat gatcatcggt cgtgggggtg gttcgattga
    20221 ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg atttatcaag ctaatacgcc
    20281 ggtgatttca tctgtaggac atgaaacaga taccacgatc gctgatttag tagcagatgt
    20341 tcgggctgcc actccaacag cagctgcaga attagctgtt cctgttttgt cagaagagtt
    20401 gatgcgcatt caagaacggc aagctagatt gcagcaggcg tttgtacgcc agatccagcg
    20461 caaacaagaa cggttcgagc gtgctcgacg ttcctatgta tttcggcagc ctgaaagatt
    20521 gtatgaaggt cagaccatca aattagacca gttgaaacag cgattatatc aagctacaca
    20581 gcaaatttat catgcgaaag aaaaacaaac gatcgcatta agccaccaat tggaacaagt
    20641 ggctccgctt tatcgagtta aagcagctcg ccaagaaact gcttatctag aaaaacgctt
    20701 agaagagaaa atccaacaat atatgaaaca acagcagcag cgttttgctc aagctgttca
    20761 atctcttgat ttgttgagtc cattgaaaat catgggaaga ggctacagct atacgacgtt
    20821 aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc tgacgatcca
    20881 ttatcaagat ggaatcgtta aaggacaagt acaagagata gacaaaggag aataaaaata
    20941 tggaaaatcc aacttttgaa gagtcactgc aagaattaga aaaaattgtc atgcagttag
    21001 aacaaggaga cgttccatta gaatcagctc tagattcgtt taaacgagga atggaattaa
    21061 gcaaacattg ccaagataca ttagctaaag cagaagagac cttgacgaaa atgatgacag
    21121 aatcaaatga agaagttgat tttgacggaa acgaggaaag ctgagatgag attaactgac
    21181 ttttcagcta ttcatctacc tgctgtagaa aaagaaatct tgtctttttt agatgagcat
    21241 acaacggata gaggactcta tgatgcgatg agttattcag tgaaagcagg cgggaaaaga
    21301 atacgtccct tattactttt aacggccgtt gcttcctttg atgagcctat tgatgttccc
    21361 gtctatcaag tagcagctgc tttagaaatg gtgcatacgt attccttgat ccacgatgat
    21421 ttgcctgcga tggacaatga tgatcttcgt agaggaaaac ctaccaatca taaagtattc
    21481 ggcgaagcat tagctatatt ggccggagat ggtttgctta caggagcatt ccagctaatc
    21541 agtatggcac acttaggtaa ttctcctaag cttctgctgc ttcagcaatt agctgtttgt
    21601 gcgggatcac aaggtatggt tgctgggcaa gctgcagata tcgagggcga atcgaaaaag
    21661 ctttctttag aagaattagc atttattcat gaaagaaaga cagggcattt gattcgttat
    21721 gccttgctgg ctggtggcat attagcgaag caacctgaag aaatcttatt gctacttcaa
    21781 cgattagccg aacatttagg tttagctttt cagataagag atgatctact cgatgtgatt
    21841 ggtacaacga aaacattagg gaaaacagct ggtaaagatg aacgtatgga aaaaaatact
    21901 tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt tggagattga gctgttatcc
    21961 gccaacaaga tcatagacaa attagaagag gacgtttttt cttttgatgg cgggctgatg
    22021 cgccagatga tcaagcaatt tgatgtggag aaatgaggga aagaatggaa aaagaacgcg
    22081 tagatgtatt agcggtcaaa caaggattat ttgaaacaag agaacaagcc aaacgttcag
    22141 tgatggctgg tttgatttac aacgaaaaaa acgagcggtt tgataaaccg ggagaaaaaa
    22201 ttccagtgag tagtgaatta agagtcaaag gtaaaaaact tccttatgtt tctcgagggg
    22261 gattaaaatt agaaaaagca ctcaaagcat ttgatctatc tgttgaagga aaaacattgt
    22321 tagatattgg tgcatccact ggcggattta ctgatgcagc tttgcaaaat ggcgcaaaaa
    22381 tgagttatgc tttagacgtt ggctacaatc agctagcttg gaaaataaga caagatccta
    22441 gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa acccgaagac tttaccttag
    22501 gaattccaga agttgctgtt atcgatgttt cttttatttc actaaaactg atgctgcctc
    22561 cattacatgc tattttaaaa gaagagggag aagtgattgc tttaatcaaa ccccaattcg
    22621 aagcaggcag agaagctgta gggaaaaacg gaattgttcg tgaccctcag acccatcaaa
    22681 aagttttaga agacattttg tcatttgcgg cacaaggtgg atatgatgtg ctggaattaa
    22741 gttattctcc gatcacgggg ggagaaggaa atatcgagtt cttggctcac ttgaggaaag
    22801 tacccgaaag cggaacaatc aattcagcta ttaatatggc agaagtggtt tcaaatgcac
    22861 acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa gtatgcggat
    22921 tcttctttat tataccaagg aaataagcta tcattgatta agaagaatat gctaaggagc
    22981 gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt gagtgagcaa
    23041 gatatccgaa agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat atctgttact
    23101 caagctacga tttccagaga tataaaggaa ctgaaactga tcaaggtacc ggcttcagat
    23161 ggcggctacc gttatagcat accagctgaa acaaacgaag atgtaaatac taaattggaa
    23221 aaactattaa aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt tattttgaaa
    23281 acattgccgg gaaatgcttc ggcagcagca aatttgattg ataaacgtta caagaaagaa
    23341 ttattttcga ttatcaatga tgacgataat gttttgatga ttacacgaac tgaagaccag
    23401 gcagttttat tgaagaaaga cttcctgcat tacctttaag ttgaggagcg aatgtgatgc
    23461 tacaagaaat cagtattact aactttgcga ttattccgga attgcggctt tcttttcacg
    23521 aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa atccattatt atcgatgctt
    23581 taggtctgtt agcaggagga agaggttcaa gcgattatat acgtcaagga gcagaaaaat
    23641 gtgtgcttga agggttgttt gaactaccaa aacaagaagg attctctgaa ctgatggttg
    23701 aattagggat cgaaacagat gaggacaatc tgattgttcg acgggatatg tccttaactg
    23761 gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt agctaatctg agaagaatcg
    23821 gcagttattt agtagatatc caaggacaaa acgaacatca ggaactgctc cagcctgaat
    23881 cacatcttgc tttattggat cgttttggag atgtagcttt ccaacagaag aaaaagagtt
    23941 atcagcaaga atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa gtgcagcaaa
    24001 atgaaaaatc ctatgttcag agaatagata tgctccattt ccagcaagaa gaaatagctg
    24061 ctgctgattt gcaagtcgga gaagaagaga aattaaaaga agaaagagaa aaacttagca
    24121 attatcaaaa aattgctgat ggattagctg caggttacgg agcactgacg aataatgaac
    24181 aaaacagtgt agatggtgtt ggcttggccg tttcagaact gcaagggatc gcccatctag
    24241 atgtggagta tgaagcaatt tatgaaaata tccaaagtgc ttactatttg ctgcaagatg
    24301 caatcggcga tatgagtcgt cagattgatt tattggagtt agatgagagt cgtttagaag
    24361 aagttacgca acgtttagaa ttaatccgac agttgaaacg taaatacggc gaatccattg
    24421 agtcaatatt ggcttactat gacgaaatca ccgaagagct agcttcttct gatttttcag
    24481 aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa agagcttcta cttcagcaac
    24541 aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa aaatccattc
    24601 tgcatgaatt gaaaagtctg tatatggaaa acactgagtt tgaggtacgt ttcttaaaag
    24661 aagaaaatag acaattgaat catgatggat ttgaccaaat cgaattttac attacgacca
    24721 atccaggaga gccactgaag cctttggtga aagtcgcttc tggtggcgaa ctgtctcgga
    24781 tgttgcttgc tttgaaaact attttttctt ctgagcaagg tgtgacaagt attatttttg
    24841 atgaagtgga tacaggtgtg agcggaagag ttgcacaggc catcgcagat aaaatttctc
    24901 aaatatcgaa atactctcaa gttttgtgca tcactcatct gcctcaagtt gctgcagtcg
    24961 ccgactatca atattatatt gtgaaagcag tgattgatgg acgtacgcaa acttctgttt
    25021 cagagttgaa aacaaaagaa agagaagaag aaattgcacg tatgctggct ggaagtgaga
    25081 ttacaaaact cacactcgaa catgcaaaag aactattgaa actcgcaaaa aggacctgag
    25141 ccacctcgca gtggttcagg tccttttcag cagctgatag ctatatttta tttttacatg
    25201 acactcatta cagcagtcaa tacaacagat ccaatcgtta aaatcagcat tgtccaaatg
    25261 acaaattttg ttactttagc aaaagtactt gtttgttttt tttcacgcat tgttttcaac
    25321 ctcgtcatta attagttgat tcaagtgtac aatctttttt ccatttccac aagagggctt
    25381 ttttataaaa acttcccttt ttttaaataa taggtagtta cgatacaaat aaataatgtt
    25441 aatagaacta gccatataaa ggcatcatcc ctttcagcaa taggtaactt aatgttcatt
    25501 ccatagatac tgccgacgat tgtcggtata gttaaaataa tcgttaaaga agttaaaatt
    25561 ttcataatat tattcagatt attcgatacg attgctgaaa aagtctgatt catttgttct
    25621 actagtttta attgaatgcg agaagtagtc attgcctgct ctgtttccac caatacatca
    25681 cgtaaatgat ttttcaaagc atgattttct tggaaaagtt tattttgcgc tagttctttc
    25741 aatgcatcta aattagcaga agtcgcttct ctgaaataaa cgaggctttt ttggatatcc
    25801 atgatttgat aaagttgttt gttttcagtg gatacttgaa gtttggcttc caaagtatcg
    25861 cattgagatt gaagcgtagc taaataactg ttgtaagaga gtgtgacctg ccaaatgaat
    25921 tgaaagaaga tattgagatt aggcgaaata tcactttttg gaaaggtctg gctaaagact
    25981 ttctttaaaa acagtggttc atgattcgta attgtgataa gcttcttttt gggcgtcaaa
    26041 ataatagaga gcgggtatgt atccacttga aaataaccgc taggacttgt tttagcaaaa
    26101 ggatattgta acaataataa cgctggcttt gtcaactttg attgattgaa ttcttctgta
    26161 cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat ttcaaattga
    26221 tgtgtcagct gatgaatctc ttcttgatta ggacgttcta cacaatacca gatcgtttgg
    26281 tcagtttcct ttccggtatt acgcagtttc tcattatcta atgtaaaata tttgatcatg
    26341 attttcctcc tgttgcctgt ttcttttatt ataacggata gggaattgga atacatggaa
    26401 tgtgtttgta agatatagaa tataactgca taaatttaaa taaaatgaaa cagttgtgta
    26461 aagtatatac agtatacttg aagcaacgat aaaaaaggag tttacgttca tgggcacatt
    26521 cacaccactt atcttgtttt ggtatattta cccggtaatc gttttgtttg gttgccaatt
    26581 tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata aaagcccctg atttggcgat
    26641 tccttttttg tggggcggta tgcatgcatt gtctagaaat acagggacaa tttctatctt
    26701 accgtttttc tttatttctg ttttgctaat gggaatttgt attgctgttt tccaagcata
    26761 ttattatgaa gaaattattt atccgcgtta tttcaagatg acttggcgtt tggtttttct
    26821 gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac atactttctt acctatgaaa
    26881 aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt gcagcttgat ttttaaaaaa
    26941 atgtttatga ataaaaaaag ttgttcccgg atttcccaga aaaggtggta gaaagtgggg
    27001 gattgtggta gactaatttt agttagtgga ggaatggggg gctttcaatg ttgatgggtg
    27061 aattccaaca taatattgat gcaaaaggac gtttaatcgt gccctcaaaa cttcgtgaag
    27121 aacttggcga aaagtttgtt ctgacacgcg gattggatgg ctgtcttttc ggctatccga
    27181 tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat gccgttggct aaaaaagacg
    27241 cacgtacttt tgtgcgattc ttctattcgg ctgccactga atgcgaactt gataaacaag
    27301 gacgaatcaa tatacccagc acgttaagaa attatgcagc tttgacaaaa ggttgcgtag
    27361 tcatcggtgt atctaaccga attgaaattt gggatgagac acgatggcaa gatttttctg
    27421 cagcagcaga agagaacttt gacgaaattg ctgaaagtat gattgatttc ggactataag
    27481 aataggaaga ggctgactaa tgactgaaac atttcaacac tatacagtca tgttgaaaga
    27541 aacggtagat ggattgaaca tcaaaccgga tggcgtttat gtagactgta cactcggcgg
    27601 tgccggacat agtgaatatt tattaagcca actaagttct aaagggcatc tgtatgcgtt
    27661 tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt ttagcttctt atgtcgaaca
    27721 aggtatggtc acatttatca aagcgaactt cagagaatta gaagaacgct tgagtgagca
    27781 tgtagataag gtagacggca ttttgtatga tctaggggtt tcctctcctc agctagatga
    27841 agcagaacgt ggatttagtt atcatcaaga tgcaccgttg gatatgcgca tggatcagtc
    27901 agctccttta tccgcttatc acgtaattaa tgaatattcc taccatgaat tagtaaagat
    27961 tttcttccgc tatggcgagg agaaattttc caaacaaatt gctcgtgaaa tcgaacgagt
    28021 acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc gaaatcatta aacaagtcat
    28081 cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa agaatttttc aagctgtgcg
    28141 gattgcagta aatgacgaac tcggtgccga agaggcctct ttagaacagg caatccggtt
    28201 attgaaagtc aatggaagaa tcagcgtaat cacattccat tcattggaag acagaattgt
    28261 caaaagtatg tttaaagaat atagtacggt acaagatcta ccaccgggac ttcctgtcat
    28321 acctgacgaa ttccaaccag aactgaaagt cattactcgt aaaccaatcc ttccttcaga
    28381 ggaagagcta acagaaaaca atcgatcacg cagtgcaaag ttacgtattg cagaaaaaat
    28441 aagaatgaaa gaggagtaga atgatggcag aattaaagaa aatgcaagat tacgaatatg
    28501 atttacctga attggtggat cagccggaac aacaaatgcc tgattcttct tcgaatcaag
    28561 aaatcattct tccacaatca ccggcaaaac gattaaagca gatttctgct ttggaaaaaa
    28621 ttgctgtggc aagcgtggcg attgctattg ttgctttatg tgttttaaca gttatgctgc
    28681 gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat acaaacagaa atcaatgata
    28741 aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga actttctcgt actgaacgga
    28801 ttaaacaaat cgcagaagaa aaaggattgt ctattaatga cgacaattta aggaaagtga
    28861 agtaaatgag tcttaaaaat aaaattcggc ggtttatgga aaagaaaaat ctaaatccga
    28921 tgaacaatcg caaaaaagta ggtatcattt tatttgctac gagtattgga ttgttctttt
    28981 tatttgccgt ccgtttctcg tatatcgtta ttggaggtca tgttgctggt acctcattag
    29041 ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt aaaagcaaaa agaggaacga
    29101 tttatgatcg taacggtgtg gcgctcgcag aagatgcctc ctcttattca attaaagcga
    29161 tactctcaaa aacatatacc tctggggata aaaaactgta tgttgaagaa aagaattttg
    29221 ataaaatagc agaaatcctg cataagaatc tttctataga caaaaaagat gcactgaata
    29281 ttttagagga cggcgcaaaa aaagagctgt atcaagttga atttggtagc tatgggaaaa
    29341 atatctccca agaaaccaag caaaacatcg aagcggacat gaaaaaagaa ggtgtcgctg
    29401 gtctttattt tgtcgaccat caggctcgga tgtatccaaa tggcgtcttt tcttctcatt
    29461 ttattggata cgcagttcca gacaaagatg agaatggctt agttggtaaa ctaggtttgg
    29521 agtctgctta caatgatatc ttgagcggta aagatggtaa gattatttat caaaaagata
    29581 attttcaaaa tcctctgcca ggtacagtgg cagaagaaga aaaagctgtc gacggacaag
    29641 atatctacac gactttagac agcagactac aaagttattt agaaacactg atggatcaag
    29701 taaatgaaga atatcagcca gaagaattga cagcagtcct gatgaaagca aaaaccggag
    29761 aaatcttggc aatgggacaa cgacctactt tcaatcctga aacaatggaa ggattgactg
    29821 gaaaagatgc tatctggcgt aatttcttgg tacaagatag ttatgaacca ggatcaacca
    29881 tgaaagtttt cactactgca gctgctatcg aagaaggaga attcaacgag aatgaaacct
    29941 tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa tgaccatgac tttggtgaaa
    30001 aaggtgtgct gaccatgcgt caagctcttt catggtcaag taacgtcggg atggtcatac
    30061 tggaacagcg attaggcgga cgatggtata actatttaca aaaactggga tttggacaaa
    30121 gtactcattc tggattagat gatgaagtaa acggtgcttt acctacatta aatattgtcg
    30181 accgagcaat gagtgcttat ggacaagcgg taggtgtgac gaatttccaa atgatgaaag
    30241 ctttcacttc tattgcgaat aacggtacaa tgattcaacc tcgatatatc agcaaagtgg
    30301 tggatcccca aactggagaa gagcgaacaa ctcaaacaga agttctaggg caaccttttt
    30361 caaaagaaac aacagaaaaa gtccgtgaat atatgagaga tgttgtcgaa agcgaaaact
    30421 acggtagtgc ttatggtgta tatagtgtac ctggatacaa tgtatcggca aaaaacggaa
    30481 cagcccaaat cgcttctgat acagggggat atcaaacagg agatacagcc tacctgtatt
    30541 cgattgtgga gatggttcct tctgaagatc cagactatgt cttgtatcta acaatgaaac
    30601 accctaagac atatgatcga atggcattgg ctaaaatcgc aaatccattg atgaaacggg
    30661 caatggattt taaagaaact gaagaagatt cagatacaga aacaaaaaca gaaaaagtat
    30721 ccgtagccga ttatcgtaat cttgaagcgg acgttgcagc agctgatgct caaaaaagtg
    30781 gactgcaacc tgtagtgatc ggtaatggta aaaaagtaca aaaacaatct actgccaacg
    30841 gtgatcagtt gatctctgga gaaaaactga tcctatatac tggtggtgat aagctgatgc
    30901 ctgacgtaac aggatggtca aaagcagata tcatgaagct ggggaaaatc ctcggtattg
    30961 aagttagttt tgatggagat ggttattgtg tcaaacaaga acttgctcca tatgaaaaaa
    31021 tcactgaaga caaacttagt tttactttag aagaataaga agagaaatag gagagaaaag
    31081 agcatggaat ggacacaagc attgattccc attgtcagca gttgtgcaat gacaattgcc
    31141 gcaatgccgc tatttattgg ttattttcaa atgaaaaagc aaggtcaggc gattcgtgag
    31201 gagggaccaa agtggcacaa tagcaaagcc ggaactccta caatgggcgg cttagtgttt
    31261 ctgatcggct ctattttgac aggaatttgg gttggagctt ggcaaaaaca gctaactcct
    31321 actcttttca tcttgctttt tgtattagcg ttatacggag taatcggctt tcttgatgat
    31381 tttataaaaa tattcaagaa aagaaacatg ggactgaact cgaagcagaa attgattgga
    31441 cagattctag ggggaatcat cttttatctt gtttaccgtt ctgaaggtta tccaggtgta
    31501 ttgaatttct tcggactaga tctaccttta ggaatcattt atggtatttt cgctctcttc
    31561 tggcttgttg gattttcaaa tgcagtaaat ctgacagatg gcatcgatgg attagttgct
    31621 ggcttaggaa gtatctcttt tgcagcttac ggtctaattg cttggcatca gcatcagtat
    31681 gatgtattaa tcatttgctt aagtgtttta ggaggtttac tgggtttctt cgtctacaac
    31741 agaaaacctg ccaagatttt tatgggagat gtcggttcat tagcgttggg cggactttta
    31801 gcggcaattt ctataatgct aaaccaagaa tggacattgc ttttgattgg tttgatctat
    31861 gttatggaaa cagcaagtgt gatgcttcaa gttacttcct ttaagcttac aggaaaacga
    31921 atttttaaaa tgtctccaat tcatcatcac tttgaaatgt gcggatggtc ggagtggaag
    31981 attgatacag ttttttggtt gatcagcatt gtgacttcgt tgatcacatt atggatcgtt
    32041 tggtaggaga tgacgggaaa tgaaaaaaat atcaacatat gaaaataaga aagtgcttgt
    32101 cttaggactc gcaaaaagcg gagtaagtgc ggccaaatta ttacatgaat tgggcgcact
    32161 tgtcaccgtt aatgatggaa aaccttttga cgaaaatccg gaagcacaag agctgctttc
    32221 tttagggata aaagtaataa caggtagtca tccaatcgaa ttgttggatg aagaattttc
    32281 attgatggtc aaaaatccag ggattcccta cagtcatcct ttagtagcaa aagcccaaga
    32341 aatgggtatt ccggtaatta cggaagttga gctagcttac gaagtagcgg aatgcccaat
    32401 tattgggatt acaggaacga acggaaaaac aacgacaaca acgatgacag gactactact
    32461 gaatgcagga gcagatcaag gaattgcacg tttagcgggt aatattgggt atccagcaag
    32521 tggtgtagcg caagaagcaa aatcagaaga taaaattgtg atggaattgt ctagcttcca
    32581 actcatggga atcacagatt tccgccctca tatagcggtg attaccaata tctatgaagc
    32641 acatattgat tatcatggta caagaaaaga atatgtcaaa gctaaatgga accttcaaaa
    32701 aaacatgaca gaaaaagatt atctgatcct taactggaat cagtcagaac tacaagaatt
    32761 agcgcaaaga acaaaagcac gcgtactgcc tttctcgaca aaagaagtgt tggaagacgg
    32821 tgtgtatgct gacgattact caatatacta taaaaaagaa aaaataatgg aaatatctga
    32881 acttggcgta ccaggtaagc ataatgtaga aaatgcacta gctgctattt ctgtagcaaa
    32941 attgtatggt atatcaaatg aagctatacg agaaacgttg cattttttcc atggcgtacc
    33001 acatcgtacg caatatgtag gagaaatcca aggcagaaaa ttttataatg attcaaaagc
    33061 aaccaatatt ttagcgacta aaatggctct aagtggattt gaaacgtcta aggtcgtgtt
    33121 attggctggc ggattagaca gaggaaatac atttgatgaa ttaattcctt cattaaaagg
    33181 aatcaaagca atggtcgtat ttggccaaac aaaagaaaag ttgatggatg ctggaaaaaa
    33241 agcaggcatc gagacaatag taacagccga ttctgtagaa caagcagttc ctttagcatt
    33301 agaaaacagt acagatggtg atgtagtttt attatcacca gcgaatgcca gctgggatca
    33361 atacccaaat tttgaaacta gaggaaacag attcatggaa gctgtgaacc gattaaaata
    33421 gaaaaagtag aagcgagcga taaaaaatga aaattttggt tacaggcgga ggaacagggg
    33481 gacatattta tccagctctt gcctttgtca attatgtaaa gactcaagaa ccgaatgctg
    33541 aattcatgta tgtgggagca aaacgaggtc tagaaaacaa aatcgtccct gatacaggaa
    33601 tgcctttcca taccttggag attcaaggat ttaaacgaaa aatttccatg cataacatca
    33661 agacggtccg attgtttctt aagagtatcc gtcaagcaaa aaaaatcctc agtgattttc
    33721 aaccagatat cgttatcggt actggaggat atgtttccgg agcggtagtg tacgctgcag
    33781 ccaaaatggg tattccaaca gttatccatg aacaaaacag tgtaccaggt gttaccaata
    33841 aatttttaag ccgatatgtg gatcggatcg ctctttcatt taaagatgcg gcttcctttt
    33901 ttcctgaaaa caaagcagtt cttgtaggta atcctcgagc acaagaagta gcagatacta
    33961 aaaaatcaga tattttgaaa acatttgatt tggatccaga aaagaaaaca gtcttgattt
    34021 ttggcggtag tcaaggagca cttaaaatca atcaagcagt atcagttttt ttaagtgaat
    34081 atattccttc ttcttaccaa gttttgtatg cttcaggaga tagatattat gaagagatcc
    34141 taaataaaat aaatcctgaa atacaaaata ttagtgtcca gccttatatc aaaaatatgg
    34201 cagaggtcat ggcttccagt gatctgctgg tggggcgtgc tggtgcaact tcgatcgcag
    34261 aattcacagc tctggggtta ccggcagtgt tggttcccag cccatatgtt acgaatgatc
    34321 atcagactaa aaatgcaatg agtcttgttc acgcaggagc agcaaaaatg attgcagata
    34381 atgaactaac aggtgaatca ctaagtcaga cagtcaacga aatcatgggt gatgaagaat
    34441 tgcaaaagca aatgtgccga gcttctaaag aacaaggaat ccctgatgct tcaaaacgtc
    34501 tttatgactt agtaaaacaa ataatcaaaa aataaaggcg gtggtcttag tgagtaagaa
    34561 aaaagaacca aaaaaattgt ctcatccgtc ttctgatgaa aacaatttga ctccttggca
    34621 aaaggctaac cgaaaatatc tggcagaaca agagatgaaa aaccaagagc caaaaaaaga
    34681 agacataaca ttagatacac ccctttcaga ttctgaagaa gaaaaaacgg ttcaagagga
    34741 acagcaagaa gaaagccagt ccgagaaaaa aatcgaattt tttgaagaga tcaagcaaaa
    34801 gaaaaacggt ggaccatata acggttcttt tttgaaccgg ctgcccaatt taaaaagtca
    34861 acggcacaaa gtactatttc gtcggttagc tttgatcatc actgtattgg gtataccgct
    34921 gatttttttg atctattatg tttctcctta tagcaaatta caagcagttg cagtatcagg
    34981 caataagaca gtcaacagcc aagaaatcat ctctgataca aaattgtcat taggagaaaa
    35041 tgtttgggga cagtattttc atcgatctac ttatatcgat cgccttaaaa aagcacagcc
    35101 aagaatcgaa acagcaaata tacatttcaa agggatgaat gaattcgatt tggatgtaac
    35161 agaatataaa gagattgctt tgatcgcaaa aaacaatcaa tactacccag tgattgaaaa
    35221 tggaacagta ttagatgaaa aagtagcaaa tcctacaaaa aatctcccta tcttggaaga
    35281 gttcaaagac tctgcaaaaa tcaaagaatt aacaaagcaa tacaatcagt tatcatcaga
    35341 attacaaaaa gcaatttctg aaatcaagta cacgccaaga gcaagcaata aaaatctgat
    35401 ccaattgaat atgaatgacg gcaatcaagt tattgtaaat atcaataatc ttgcgaacca
    35461 aatgaaatat tattcgcaag tagccaaaga tatggatgaa aaaggtgtga tcgacatgga
    35521 agttggtatc ttctcgcatc cttacggtag cactcaagaa acggaagaat cgggccaatc
    35581 ggaagaaaca actgaaagtg aagaaaatgc ggatcaaaca aacgaatcaa gtgaagaggc
    35641 agaaacgcaa aaagcacaaa cacctggatc gtctagcaca aacggatcat ctgattcaaa
    35701 tgattcaagt gaaacaagtc aaatagaaga taatccaagt acaactccgt caagtttaaa
    35761 tgaaaattga taaaacaact ttaaaaagta agaatgtact ttctaaaaac cagtaattta
    35821 tggtagaatt ggaagcagtg aatatatcta ttaataagta tattttaaaa cggacgctag
    35881 ttgttcgctt gtatatggaa tagtaagtag gagggaatcc cattcatggc aaaaacagga
    35941 atgtatgtag gccttgatat tggaacgaca tctgttaaag ttgtcgtggc tgaatatatc
    36001 gacagccaaa tgaatattat tggagtagga aacgcaaaat cagaagggat taaccgaggc
    36061 attatcgttg atatcgataa aacggtccaa gcgatacaac gagcagtacg acaagcagaa
    36121 gaaaaagcag gtatccagat caaaggcgta agtgtcggtt tgccagcaaa catgcttgaa
    36181 gtagaaaatt gccagggaat gattgcagtt aacggagatt caaaggaaat caccgatgaa
    36241 gatgtcagaa atgtggcttc agctgccctt gttcgttcaa tacctcctga acgtcaaatc
    36301 gtgtcaatat tgccacaaga ttttactgtc gatgggttcg agggaatcaa agatcctcgt
    36361 ggaatgatcg gcgtaagatt agaaatgtac ggtctgttgt ttacaggacc taaaacgatc
    36421 atccataaca tacgtaaatg cgtggaaaat gccggattaa tcgtcaacga aatggtgatt
    36481 actcctttag cgctgactga atcaattctt tctgacggag aaaaagattt tggtacgatc
    36541 gtcattgata tgggtggcgg ccaaacaaca acggctgtta tgcatgataa gcaattgaag
    36601 tttaccaatc ttgaccaaga aggtggcgaa tttgtcacta aagatatctc tattgtatta
    36661 aatacgtcat ttaacaatgc agaagcgttg aaaataaact acggggatgc atacccagaa
    36721 cggacatcac cagatgaaga atttccagta gatgtgattg gtcagtcaga accagtgaaa
    36781 gtcgatgaac gctacttgtc agaaattatt tcagctcgaa tggaacagat tttcaacaaa
    36841 gcaaaagatg cattggacca aatcgaagca ttagagttgc caggtggcgt cgtattgaca
    36901 ggcggagctg ctagtcttcc tggtgttgtg gacttggcac aagaaatctt cggcgtaaat
    36961 gtaaaattgt atgttccaaa tcacatgggg ctgagaaacc cggtatttac aaatgtaata
    37021 agtatcgtgg attattcagc aaacttgagt gaagtgtatc aattggcaaa aattgctgtt
    37081 acgggtgaaa cctcagcagc tcgtcaaatg gtagttgaac aagaaacaac aaacacatat
    37141 gagagctatg aagcacctga agaaacaatc tacgatgagc cggaaccaaa agagtccgga
    37201 gaaaatgtaa aaaataaaat caagggtttc tttacaaata tttttgatta attcatcctg
    37261 ggaggaataa gatatggaat tctcaattga taataatatc aatgacggcg cagtaatcaa
    37321 agtcatcgga gtcggtggtg gcggtggaaa tgctgtaaac cgaatgattg aagaaaacgt
    37381 taaaggtgtt gaattcatta cagccaacac agacgtgcaa gcattaaaaa attcaaaagc
    37441 tgaaacagtt atccaattag gccccaaata tactcgtggt ttaggtgctg gttctcagcc
    37501 agaagttgga caaaaagcgg cagaagaaag tgaacaatcc ttacgcgaag ctttagacgg
    37561 tgcagatatg attttcatca ctgcaggaat gggtggagga accggtacag gtgctgcacc
    37621 aatcgttgca ggtatcgcga aagaattggg cgcattgact gttggtgtcg tgacacgtcc
    37681 attcactttt gaaggaccga aacgtggccg ttttgctgca gaaggtatcg ctcgtttgaa
    37741 agaaaatgtg gatacattat taattatctc aaataaccgt ttattagaag ttgtagataa
    37801 aaagacaccg atgcttgaag ctttccgtga agcagataat gttttacgac aaggtgtaca
    37861 aggtatctct gatttgatca cagcgccagg ttacgttaac cttgactttg ctgacgtgaa
    37921 aacagtgatg gaaaaccaag gtacagcatt gatggggatc ggtgtggcta gtggagaaga
    37981 tcgcgtagta gaagcaacga aaaaagcaat ctcctctcca ttgcttgaaa catctattga
    38041 tggagctgaa caagtacttc ttaacatcac aggcggtttg gatatgacat tgttcgaagc
    38101 acaagatgct tcagatatcg tagcaaatgc agcaactggc gatgtaaata tcattctagg
    38161 tacatctatc aatgaagaaa tgggcgatga aatccgtgtg acggtcatcg caacaggtat
    38221 cgatgaatca aaaaaggaac gcaagtctag ccgaccagct agacaagcac aaatgcaatc
    38281 acctgctcaa aagactgtat tagatatgga tcaagctaaa ccaacttctt cagaagaaga
    38341 aaacagtttt ggcgattggg atatcagacg tgaacaaaat gttcgtccta gagttgatga
    38401 ttccaatttt gacaatatcg aaaaaaaaga atttgataca ttcaaccgcg aagaaacaaa
    38461 atctcatggc gatgatgaat taaatacgcc accgtttttc cgtcgtaaaa aataaaggag
    38521 aggaagagca catgattgct gacaacttgc agaaaatcaa gcaagaaatt caggattcgt
    38581 gtgctcttgt tcatcgtgat ccgaaagaag ttacattagt agctgtaaca aaatctgttg
    38641 aatccgctca agctgaagaa ttggttagag ctggcgttat gaattgtgca gaaaaccgag
    38701 tagataaatt attagaaaag aaacaagatt tatttgattt tcctgaaata aagtggcatt
    38761 taattggtaa tttacagcgc agaaaggtaa aattgattgt aaatgaaatt gattactttc
    38821 atgcgttgga cagtttaaga ttagcagaag aaatccagaa gcggttagag aaagaattat
    38881 cctgttttat tgaagtaaat gtttctggtg aggaatcaaa acatggaatt tcaccagatg
    38941 agcttttacc gtttgtggaa tctttagctg cctgcacaaa aatcaaggtt gttgggttga
    39001 tgactatggc gcctaaagaa gcttctgatg agcaaatcca taaagtgttt ggtacgctga
    39061 aagaattgcg tgatcttgtt caagcaaaag gttatgccca tgcaccatgt agtgaattaa
    39121 gtatggggat gagtcaagac tttcaaattg cgattgaaga aggtgcaaca tttatccgtg
    39181 taggaacagc gctattcaaa gacgaataaa ggaggaacaa aaatgccgct tttaaacaga
    39241 ggagcaattg ctaatttttt cggattagct gatgaggaag agtacgaata ccaagattat
    39301 cctgaaacaa caaaaaaggc tgtacagcca actaaaaaaa atacaacaca gaatcaatca
    39361 actcattctg tatctgctgc gaatactcat gaaaaacaaa aaacggcgac ttcacgtcct
    39421 gtaacgcatg aaagtattca gccggctgaa aagaaaaaag taattgaact acatcaatca
    39481 cctacaaaaa ctgtagcgaa agaaagaaca gcttcaagaa cctctacaca acctaaagca
    39541 aatcttgctg gaaaaatcac tgtaatggaa ccaagagctt attctgaagc tatggcgatt
    39601 gctaaacgaa tcattgctgg agaagcaacc ttagtgaatt ttcatttagt agaagaaaaa
    39661 caagcgagaa gaatcgttga ttttctaaca ggaacagttt atgcactaga tggagatatc
    39721 aaacgtgttg gagatgagat ttttctttgt acaccgactg ggatggagat tgacagttca
    39781 accgctcaat cttttgcaga tgcgaatttg tttgatctgt aagaggagga actacgattt
    39841 atactattgc actattactt catttacttc aacgggcagc ctatttctat agcattatct
    39901 tgatggtata tgcattgctc tcttggtttc caggcgggta tcaatcaggg ctaggacgat
    39961 tattagctaa gatctgcgaa ccgtatatca gtttgttcga tcgtctgcct ttgaggattg
    40021 gacccttgga tttcacaatt ttaggggcaa tcctgatctt gaacttagcc gtacaaggtt
    40081 tagcacagat cgttatatgg ataactgtcc atttagttta ttgattgaag caacaaaatc
    40141 tttattctca gaatagatag gaggggaaaa ttttggatgc aaatgtatac cagcactttc
    40201 gggcagatga acgtacattt atcgattcgg taggcgattg gattgaacag gttcaaggac
    40261 aatatgcacc ctatctgacc gaatttcttg acccaagaca atcctatatt ttagaaacac
    40321 tgattcgaca aagcagtgat ttgcgtttca tgttttatgg cggatatgaa caggcagaaa
    40381 gaaaaagatg tttgattttc cccgattatt acgaacctgt agaggaagat tttgaaacag
    40441 cattgtatga aatccattat cctagcaaat ttgccagcct aagtcatggg aaaatccttg
    40501 gaactttagt gggaacgggg atcaaacgct cttactttgg cgatatcatc tcggacggtg
    40561 atcgctggca agtatttatt gcaaatgagg tggatcattt tgttgctgca caagtaacaa
    40621 agattggaaa ggttgctgta cgcttggaac caatcaaata cacagaaatg attatgccga
    40681 aagatagctg gacacaagaa agaggaacag ttagttccat gcgactagac agtgtgatct
    40741 ctgagattta taatatttcc agacagcggt ccaagcagct gatcgaagct ggtaaagtaa
    40801 aagtgaactg gacagaaaat acaagacctg attttgtact tgaactatta gatattattt
    40861 cagtcagagg ttatggcaga gtccagatcc aagaacttga ggggaagacg aaaaaagaaa
    40921 agtacagggt actgttcggt gtattacgta aataaaataa aaaggtggtt tactcatggc
    40981 tttaactcca ttagatatcc aaaataagac attcccgaca aaaatgcggg gatataatca
    41041 agacgaagta gatgatttct tagatttagt tgttcgtgac tatgaggaat tgactcaaag
    41101 aaatcgagaa ttagaaaaag cagtcaaaca ttctgaagaa aaacttgagt acttcaatga
    41161 attaaaagat gctttgaacc aatcaatcat cgtagctcaa gatacggctg ataaagtaaa
    41221 aacaagtgca agcaaggaat ccgaagtgat cgttacttct gcacaaaaca aagcagatga
    41281 gttagttgcg aacgctgaaa aacgtgcaca tcaattaaca actgatgcag aagaaaaagc
    41341 aagaaaaatc ttgacagatg caacagaaaa agcaagacag cttgcgacag aaactgaaga
    41401 tcttaagaaa aaaacacgag tattccatca acgtatcagc ttgatgcttg agtctcaatt
    41461 agaacaagtg aaaagtccgg aatgggatga aattttacaa ccattctcca gctacgtgac
    41521 tgacagccat gaagtgatca aagaagtttt agcaaaacag cttgacaatg aaaatgatac
    41581 tgatgtaaac tcagacacag gagtcataga agcacctatg acggcatttg atacacaggc
    41641 tattgatatc tcgatcgtgc ctggtgaaaa tgaaaacgag tagaacagaa aagcactttg
    41701 ttattcttaa agcgagccga tgagagtgga agttcggtaa gacactgcat ggtgaaagat
    41761 cctctacttg agacctgctt ttccgaaatt agagtaagaa gcagcggctg acctcgttat
    41821 tggtcacaga gagtttgcac tagatagatg caactaaatt tgggtggtac cacggcatgt
    41881 tcgtcccttg cggctgaaca tgcttttttg tgtccggaaa acaaataaag gagtgtatgg
    41941 ttgaatgaaa atgaaagaaa ccttgcaatt aggaaaaaca aaattcccta tgcgtggtaa
    42001 tttgccaaac cgagaaggcg agtggcaaaa agaatgggaa gaaaatcgaa tctaccaaaa
    42061 aagacaagag ttgaacgaag gaaaacctag ctttgttctt catgatggac ctccatatgc
    42121 caatggtaat atccatttag gacattcatt gaataaaatc agtaaagaca ttatcattcg
    42181 ttcaaaatca atgtctggct atcgtgcacc atatgttcca ggatgggata cacatggatt
    42241 gccgattgaa caagtattgg ctaataaagg cgtgaaacgt aaagaaatgt ccatggcaga
    42301 atacttggaa aaatgtcgtg aatatgcact ttcccaagta gataagcaac gagcagattt
    42361 caaacgccta ggagttgcag gagattggga acatccttat gttacattag atccttctta
    42421 tgaagctgca caagtacgcg tatttggtaa aatggctgaa aaaggctata tttacaaagg
    42481 attgaaacca atttactggt ctccgtcaag tgagtcttct ttagcggaag ctgaaattga
    42541 atataaagat gtaaaatctc catctatttt tgtagctttc aatgtcaaag acggcaaaga
    42601 tattcttgat gaagatacag cttttgtcat ttggacgaca acaccatgga cattgccagc
    42661 aaaccaaggt attgctgtaa atccaaaatt tacgtatgta ttggtggaag ccgatggccg
    42721 taagtttgtc gtagcgaaag atttgctaga aacagtccaa tcagaaatcg gttgggaaaa
    42781 tgtaacagtt ctaaaagaat ttgctggaca agaaatggaa tatatgactg caagtcatcc
    42841 attctatgaa cgtgaatcac ttgtgatttt aggcgatcat gttactttag acgctggtac
    42901 tggattagtc catactgctc caggacatgg ggaagatgac tatatcgctg gtagcaaata
    42961 taaactatct gtagtgtcac ctgtagactc aaaaggattg tttacagaag aagcacctgg
    43021 atttgaaggt atcttttatg acaaagcaaa tcctatgatc actgatctat taaaagaaaa
    43081 aggagctttg ctgaagctag atttctttac tcatagttat cctcacgatt ggcggacgaa
    43141 aaagcctgtg atctatcggg caactccaca atggtttgca tctatcgata aattccgtca
    43201 gaatattttg gatgaagtag aaaaagtaga gtgggttatc ccatggggta aaacacgttt
    43261 gtataatatg atccgtgacc gcggcgattg ggtaatttcc cgtcagcgtg catggggagt
    43321 accacttccg attttctatg ctgaaaatgg agaagcaatc atcacaccag aaacaatcga
    43381 acatgtggca aaattatttg ctgaacatgg atcaattatc tggtttgaaa aagaagcgaa
    43441 agaacttctt cctgaaggat ttacccaccc aggttctcct aatggcgaat ttacaaaaga
    43501 aaaagatatc atggatgtct ggtttgattc tggttcttct catgaagctg ttttacgtca
    43561 gcgaccagaa ttatcattcc cagcagatat gtatttagaa ggatcagacc aatatcgtgg
    43621 ctggttcaac tcaagtatca cgactagtgt ggctatcaat ggtgtagcac catacaaatc
    43681 agttatttct caaggatttg ctttagatgg tgaaggccgt aagatgagta aatctttagg
    43741 aaacatcatt gcaccagaaa aagtgatcaa acaatttggt gcagatatcc ttcgcttatg
    43801 ggtatctagt gtagattatg aagcagatgt tcgtgtttct atggatatct taagtcaagt
    43861 ggcagaagta tatcgtaaaa tcagaaatac gatgcgtttc ttattagcga atacagaaga
    43921 tttcgatcct aaaaaagata ctgtttcgta caatgattta cgttctgtag acaaatatat
    43981 gaccgttcga ttgaaccaag taatcaaaga aatccgagaa gaagggtacg acaaatataa
    44041 cttcatgcat atttaccgta cagttatgaa cttcttgacg gttgatctat cgtcattcta
    44101 cctggatttt gctaaagatg tagtttatat tgaagcagaa gatgattatc aacgtcgttg
    44161 catgcaaact gttttctatc aaacagctgt agctttgaca aaattattga ctcctatcat
    44221 tccgcataca gcagaagaaa tctggtcatt cctgaaagaa gaagaggagt acgttcagtt
    44281 aagcgagttt cctggttatg aagagtttgc aaaccaagaa gaattgatgg atacctggac
    44341 tgctttcatg gacttccgtg ataatgtctt aaaagcattg gaagaagcaa gaaacagcaa
    44401 attgattggt aaatctttgg aagcaaaact aactgtttat ccaaaacaac aagttcgtga
    44461 aatgttaaaa gctttggatg cagatattgc acaattattg atcgtttcac cagactactt
    44521 tgaagttatg gcagcagatg aacaagtgcc agataacgct atggtatttg atgatgttgc
    44581 tattacagtg gaaaaagcag acggggaaac ttgcgatcgc tgtcgccaag ttcgtaaaga
    44641 tgtaggagta gatgaaaaac ttcctcatct atgcggtcgt tgtgcaaaaa tcgttgaaga
    44701 cttctatcct gaagctgtag ctgaaggatt cgaagaaaag taaaagggaa aagggggtgt
    44761 gacatgttct ttgtcacacc cccttttgtt atcaatcgtt taattttcca cgttcacgat
    44821 accagttatc tggttgccag atccattcga atccgtcttt ttcaagtagt tcgaaagctg
    44881 ctttaggtcc cattgatcca gctttgtagt tgggaaaatc cggttgtgtt tggtcccaag
    44941 cttttcgaat gacatccacg atatgccaag attgagcgac ttcttcccaa tgagagaagt
    45001 tcgttccatc accattcaac gcatctaata gtaatttttc gtaagcttct gggctatttt
    45061 caaccatttc tgcactattt ctaaattcca atttgattgg ttccgtttca aatccttgcc
    45121 ctacttcttt cccattcaag gataaagaaa atccttcagt tggctgaata tagatcgtta
    45181 gaacattagg tggaagtgtc gtatcgtcac atggttcatc gacagatgtt ttgaatacat
    45241 taacaggaac ttgtttgaat acgatgttga tccgagtccc tttttcagtc aaacgtttgc
    45301 ctgtacgtac atagaaagga acacctgacc aacggaagtt atcgatagtg aatttgcctg
    45361 cgacgaatgt ttcagtctct gattgagcat ccactttgtc ttcttcgcga tagcccttga
    45421 attgtacacc atccaattca ccttgagcat attgcccgcg aacgaagttt tctaacactt
    45481 cttcttcgtt ataaatgcgg atagctttca atgctttgat cttttctgta cggatttctt
    45541 tttcggaaaa tgctactggc ggttccattg caagtaatgc tacaacctgt aaaatatgat
    45601 tttgcaccat gtctttaagt gcgccactat gatcgtagta gcccccgcga tcttctacac
    45661 cgaggttttc gccaaaagta atttggatat tgtcgatata gcgattgttc cattgagcct
    45721 caaaaatatt attggcaaaa cggatagctg agatgttttg gatcatttct tttcctaaat
    45781 aatgatcaat cctgaaaata tcttgttctg ggaatacttc tctgatttgc tggttcaatt
    45841 caaaagcaga ctggtaatca gaaccgaacg gtttttctat gatcaaacga ttgaagccat
    45901 tttttgtcat aatattttgt gatttcaaat gagaaacgat cgttccaaaa aattgaggtg
    45961 ccatagccaa ataatagaca cggttaccgt ttaattgata gtgtccatct aatcggtcag
    46021 acaattcttt taatgtctga taatgcgttg aatcatttac atcatgggat tgatagtaaa
    46081 aatggcttgc aaaggtagtt gcttcttcat ctgaaggatt caatgcacgg attgtttctt
    46141 tgacaacttc acggtagtgc tcatcgctcc agggacggcg agcggttccg attacagcaa
    46201 aatgctcgcc taaatttcct tttctgtaaa ggcggaacaa agacgggtac aatttacgct
    46261 gggctaggtc acctgtaccg ccgaatatcg taaacaatac atttttttca ttagacatgt
    46321 ggatacactc ctttatattt tatcgacaaa aatcgtgctg gccgccttaa agctgacggg
    46381 gattttcttt ctatcatttt ggatagtgat cggtccttca taagctgcga tgtcaacgat
    46441 ctcatattct tcgttgatat tcaggtcaat agtgactaag taatccaaca attctttttc
    46501 atcaagcact cgtgcgatac gcacttttgt accgacagga taggactcca atgtttgtcg
    46561 gcgttcttca tggacaactt cctctttttt aggaatcgat cctccatgag gacaatgttc
    46621 aggatgattc aaatagtttt ctaaatgatc tgcaagatgt tccgaagtca catgttccaa
    46681 tacttcagca tcatcatgga cttcattcca gctatagtct aaatgttcaa ctaaaaaaac
    46741 ttcccataag cgatgtttgc gaatcagact gctagctttt tgcagtccgc ttgcagtcaa
    46801 ttgtacacct tgataagggg aatgttctac aagctcttct ttcacaagtt tagaaatcat
    46861 ttcactgaca gatgcagctg agacatctag actagatacg atttgtttat tgttgacttt
    46921 cgttgtatct ccacctagtt ctaaaatgat tttcaaataa tcttctcgat ttggtgtcat
    46981 attcgatcat cctttcatgg taaaaaacca tcattagtaa gtgtaacaaa aaaaacgtta
    47041 attgactatt tttctaaata aaaaacaaag gatttttaga cgtttttaca acaaaaaacg
    47101 aaagatgata gagaagactc attctttcgt ttataaagga tatatatata cattgggagg
    47161 gatctatttt tcagataaca acttatatga agtgtctgca gattcgtcat aaagagtttg
    47221 ttcgggataa gaaaagataa gataaaaacc atactgatca cgtacgacta aacgatattc
    47281 atctatttct accacagatg tgccaagaga ctgaccgttg atcaaaaacg tgtaatcaga
    47341 tgtaatctgt aattgttgtt tttgctggct atcactaaag aaccatgtac cttgatactt
    47401 tgagagtcct tctgactttt ccgcttgttt tttctttttc aagtaaataa aacttgagag
    47461 aacggtaatg agtgaaccaa gaaaatagat catttttttt atcaccaaat cactccttta
    47521 tgagaaaata atcgcataga catattcttt cttctttatt tcattgtacc gaataaaagc
    47581 taaaatttct ctaagacttt cgactggttt ttttactgtt tttctacaaa ttatgatagg
    47641 atacatagta ggaagaaagg tgacataatt tgaagtatca gcaagtggaa atagcttttt
    47701 ttattgaccg cccgaatcgg tttatagcac attgtatgaa aaaagacgga gaaataatta
    47761 ttgcgcatgt caaaaataca ggtagaggaa aagaggtcct gataccaggc gctgaagtgg
    47821 ctattgtatt tgctcctgga cctaaacgga aaaccgccta tgatctgatt gctgtaaaaa
    47881 agaagggtga ctggtttaat atcgatagtc aattgcctaa tcggctggca attgatggta
    47941 ttttagacgg cacgatcctt ttaccaggat taactggtcc atttacggta tacaagcgtg
    48001 aagtaactta tcttcactcc aaatttgata tttatttaga gacggtggtc ggacaaaaag
    48061 catttgttga agtcaaaggt atgacacttg aaaataaagc gattggtgct tttcctgatg
    48121 cgcccacact cagaggattg aaacatatag gagaattaac gtatgccgca caagatggtt
    48181 atgccgctta tgtcttgttt attgctcagt tcgaacatct tcatctggca acgatccatg
    48241 aagaaatgca accagcacta gctgatatgg ttcgacatgc gcagcaaagc ggagttcaaa
    48301 tcctcgccta taattgtcaa gtatcggcag atgaggtaac tgtcaagcag gccattccat
    48361 ttgatgtaga tatccctttt gaagatccta tacaatagaa agaaggtaaa ataatgatca
    48421 acttaggtat tatcggaaca aattggatta ctcatcaatt tgtccaagca gctttaagca
    48481 caaaaaaata tgaattgaca gcggtatatt ctagacattt ggaaaaagca caggaatttg
    48541 gttctcatta tgaaggggat atcgaattcg caacagattt gaagactttt ttcaatatta
    48601 ctcatatgga caccatttat attgcttctc cgaatagtct tcactttagt caagctaagc
    48661 aagcaatttt ggcaggaaaa aatgtgatag ttgaaaagcc agctttttcg acgccaaaag
    48721 aaatggatga aatcatccat ttggcaaatg aacacaatgt gtattttttt gaagcagcaa
    48781 gaaatatcca tgaatcaggt tttaaaaaga ttgccgatta cttaccatta aaagacgaga
    48841 taatcggtgc aaatttcagc tatatgaaat attcttcgcg ctatgaccaa gtattagatg
    48901 gagaagaacc gaatatcttc tcgactcatt tctcaggtgg tgcattgatg gatttaggtg
    48961 tttaccttgt atatgcagct gttgcttggt ttggaatgcc aaaagaagtc cattatttcc
    49021 caagaaagat ttctactggt gtagatggtc ttggatgggg gatcttacgt tatgagaatt
    49081 ttgatgtatc gatccagcca ggtaaaatcg gtgattctta tttaccgtca gagatttatt
    49141 ttgactcagg aacgctagtg atgaatggag tcaatgcaat tgaacaagca caatttcatg
    49201 atcgcgcgca taaagaaatc attgatttgg cggtaacagc tgcagaaaat ccaatggcag
    49261 atgaagcaga ggattttgcc aatgtcatgg ctcatccgaa agatccagct tggggattgt
    49321 tgtatgaaga gtggatcgag ctagctagaa atgtgaacca agtgatttat gatttaagaa
    49381 aaaatggcgg catcaaattc gatgcagata atgaggaaga tttttaatga acgaatttgt
    49441 acaagtattt cccgaaaatt ggcagaaaaa atatgaggat aaagggttta tctcaccttc
    49501 tcttattcaa cgagcagttt ttcagccact aagtaataaa caaagtatag tagcggtttc
    49561 tcctacaggt tcaggtaaaa cactggctta tctttggcct ctactactaa acgtggaacc
    49621 aggtgaagca agtgcgttgg tcatttttgc ttcttctcaa gaactagcta ttcaagtagc
    49681 agatgttgca cgtgaatggg gaaaagacaa agagttgaaa gtccaatctt tggtgggcgg
    49741 agccaacgtc aaacgtcaga ttgagggttt gaaggaaaaa ccagaaatct tgattggtac
    49801 accaggaaga atattggaat tgatgaaagc aaaaaaaata aaagcacatc aagtgaaaac
    49861 aatggtattt gatgaagcag accagctatt tgacgctggg aacagccaaa tcattgatca
    49921 aattcttcat caagctccaa cagagtatca gctagctttt ttttcagcga cagcagatcg
    49981 ttcccttgaa agcattgaaa aaatcactgg gaaaacgttt gagacaattg acgtgacagc
    50041 agaggacgat tctcgaaaag gactgagaca ttacttttta cgtgtaccga tacgaaagaa
    50101 agatgactac ttgagacgtc tcacgcatat agaagatttt catggtctga ttttctttaa
    50161 tcaactgact gaattaggcg taatggaaga aaaattgctg tatagaggtg taccggtagg
    50221 aagtctggcc tctgatcaga acaagctact ccgaaaagca gcgctagaac aattcaaaaa
    50281 gaaaatggtc agagcacttt tgaccacaga tgtggcggct agaggtttag atattacagg
    50341 tcttccttat gtagtgaatg cagaagttcc gttaagtgaa gaagcttatt tgcatcgttc
    50401 gggaagaact ggtcgaatgg gtaatgaagg aatagtaatt acactcgtac aagataataa
    50461 tctaagagat ttaaaacggc ttttacgtcc atcaaatata acacttgaag agattttttt
    50521 atacggaggt aagctgcaaa ctgagcagcc aaccaaagaa gatgcagctc ctgcagtaaa
    50581 taaaaagtat gcgaaaactt tttctggaaa ggagcagaac ccagaatata acaaagaaaa
    50641 aataagaaaa aaagttaaaa ataaatcaaa aaaagagaga aataaaggag caaggcgcaa
    50701 ataagcgcct ttttatgcta tgattatgaa aacgggaaca aaattggttg ccagaggagg
    50761 ggttatgtga aagaacgtca agaagagatc ttgcagctat tgacagaaaa ccaacaagga
    50821 ttgacagcaa gtgatgtagc tgagcgctta actatcgatc gaagcaatgc aagccgatat
    50881 ttaagtgaac tatacaaggc ccatcatatt gtaaaaaccg ctggccgacc agtcgtttac
    50941 tctttgccaa cggaaaagtc aaaatctgat gaagtgcatg tcgactcttc tacgcaagtg
    51001 acttttgaaa cactcgttgg agaaaatgat tctttaaaag tcagcataca gcaggcaaaa
    51061 gctgcgattc tttatccacc gcgggggttg catacgatta tttttggaga aactggtaca
    51121 gggaagtcga tgtttgctga atgtatgtat cattttgcaa tcgattcaga gatgctttct
    51181 gcagatgcgc catttgtttc tttcaattgt gcggattatg ctcaaaatcc gcaactgctc
    51241 tttggacaca tctttgggat caaaaaaggg gcttacacag gagcagctca agatagtccc
    51301 ggtctaatag ccaaagcaga cggcggtata ttgtttttag atgagatcca ccgtttaccg
    51361 ccggaagggc aggaaatgct gtttactttc attgataaag gtatttaccg tccgcttgga
    51421 gaaagtgcac aagtacatga agcatctgtt cagatcattg gggcgacgac tgaatcttca
    51481 gaaagtttct tatctacgtt caatcgacgg attcctatgg caatcacctt gccgaatctt
    51541 gcttcacgtt cactggatga gcgttatgaa atcatctctc tttttatcaa acaagaagcg
    51601 aatcgtttga atcaacgtat cgatatcgaa aaagaagcga ttcttgcctt catgctttat
    51661 gatgcggaag gaaatatcgg tcaagtgaaa cgagacttga aactagtctg tgcaaaatct
    51721 tttttacatt accgtactca tcatgagaaa aagctaatca ttcgaaaaga agaactttcc
    51781 cttcaagtac aaaaaggatt actgaaaatc aaggaaatac cggaacgtct cgaccgattc
    51841 atggacagca aaagtcagta tttgacattt gaaccaggat ttgctgatgt tgtctggtca
    51901 caagatccag aacgaaacat gcaggtctat aacgatattg aggaaaaagt cacttctctt
    51961 tccgaaaccg gtgtggagaa cattgatcta gagaccctca tttcaaaaga tgtggatgct
    52021 tatttccaga catatgtcaa agaattgact aaaagttcga ttcccaaaga tcttttgcca
    52081 gatgaaatct ggcggttaac caatcaattg tatgatgtgg ctgaaaaacg gttagaccgt
    52141 atttataatg aaaaagctcg ttttgctttt gctttgcatc tgcaaagtac attggatcgt
    52201 gtgagagaag gtcatatgat cgtccaccca gatttgaata acgtacggaa aaattttaag
    52261 agggaattcc aggtagcaat cgatctttca acgatcattg aagaagaaga acaagttgag
    52321 attccttttg atgaaatcgg ctttattagt atgtttcttt ctatcaatct aggagaaagt
    52381 gagcctgtta aagaaaacaa agtagatgtt ctcatattga tgcacggcag acagactgct
    52441 tcaagcatgt tggagatggc acaagaacta ttaggaacaa aagtagggaa agcttttaat
    52501 atgccactgg ttatggaagt tcaaacaatg tatgaagaag tgttacaata tgtcaaaaac
    52561 tatcaagagc atttgacgaa tggcctactc ttgttaacag atatgggatc attaaacacc
    52621 ttcagtgatt tgatttatga agagactggc attcggacga aagctatcac gatgacaagt
    52681 accatgattg tgatagaagc gatacggatg gcaagtgttg gaagaagctt agaagatatt
    52741 tatcaaaata tccagctttc ctttgaatcg atcgtacgtg atcagttcaa agcggaaaag
    52801 tctgaaactt tacagcgtaa aaaagccgta atcgtgacat gctttactgg tgaaggagta
    52861 gctgccaaat tatatcagcg tattatgcca gtgattaatc aaaataaggt agaactgatc
    52921 cagatgcagt ttttagaacg tgaatcattc aaaaaacaca tcgatgcctt attggaagaa
    52981 tatgagatca aagcaattgc gggaacagtt gatgttgatt atcaaaatat cccatttttt
    53041 tcagcctatg acgtttttga tgatgaaaaa ctaaatattt taaagcgtat agctgatgat
    53101 gagatcccga cagctaaaat tgtcgattca cttgctggat cattaagcga tgtcttttct
    53161 attgatgaac tgatccgttc attgcaaaag acagttcagc agatccagac cgatttgtcg
    53221 attatcgtgg aacctggagt agatactgga atcgttatcc atttagcctt catgttagac
    53281 ggattgatca aacaagaaag ccatcggcaa tttgagaaac tgtctatttt ctcaaaaatt
    53341 tatcgtttgg aaatggatat cgttcgggca aacttgatgg cactagaacg aaaatatcag
    53401 gtaaaaattc cagaagatga tatcgcattt cttacacaaa tgtttcttga aaataaaata
    53461 aaaaaatgaa cacactaatc atactgttta cacactaaac atctgtgtgc gcactataaa
    53521 aaagagctaa aaagcatgaa aacgctattt cagctctttt ttttattggc atgcttcttg
    53581 cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa atggcaaaaa aaacaatcat
    53641 gttagtttgt tcagcaggga tgagtacgag tttattggtc acaaaaatgc aaaaagcagc
    53701 agaagaaaaa gggatggaag ccgatatttt tgcggtctct gcctcagatg cagataataa
    53761 tttagaatct aagaatgtcg atgtattatt attaggtcct caagtacgtt tcatgaaagc
    53821 tcaatttgaa caaaaacttg cgcctaaagg tattccatta gatgtcatca atatgcaaga
    53881 ctatggcatg atgaacggag aaaaagttct tgcacaagca gaaaatctaa tgaaataaat
    53941 caagtatgca gttgggggta taacaacgtg gaagatcaac aaaatctaga agccatcatg
    54001 gggttaatca tgtttggcgg taatgcaaag agcgatgcaa tggaagcgat tgcggctgct
    54061 aaaatcggtg attttgagtt agcggatagg aaaatcgctg atgcagaaga atcacttata
    54121 caagcgcatc atgcacaaac agacatgttg acacaagaag cacaaggaaa tcatatgcaa
    54181 gtgacattat tgactgttca cagtcaagat cacttaatga catctattgc gtttactgat
    54241 ttagcaaaag aaatcatcga actttatcgt cgcgtataca gcgaataaat acaacttctt
    54301 ttttgaacgg aagaagaaaa atttttgaac ggatttttta ttttgttata aggagaactc
    54361 tctttatata aaaaacttat aggaggaagt aaaaaatgaa cggactatca agttggatgg
    54421 agaaatacat cctgccggtt gccgcaaaaa tcggagcgca aaaacattta gttgctttgc
    54481 gtgatgcttt tatcggcaca ctgcccgcaa caatggcggg atcagtcgct gtaatgatca
    54541 atgcaattat tcgtgattta ccgcaacagt ttaacagtac ctatgctgac agtttagcag
    54601 cagacaaagg aatctttgcc gtattgaatg tcatcatcgg aatcaatggt tttgtctgga
    54661 acggtacact agcaatcgcc ggattgatct ttgccttttc ttgggggtat aatcttgccc
    54721 gagcttatgg agtaaatgat ctagctgggg ggatcgttgg tttagctacc ttgattcaag
    54781 gtatttcctt tggtccagca ttatcagcag cattagatgt gaatgttcct tcaaatgtcg
    54841 cagatgcaat caatagttct gagattggtg caacgattgc agatggcaca ctgtcagttg
    54901 gattatgggg ttggctaaaa ctagatcatt tgaatggaaa cgcttatttt acagttatga
    54961 tcatgggagc tatttcagtc attatttttt gtaaattgat gcttgccaac attacgatca
    55021 aaatgcctga ttcagtacca ccagcagtat ctaaagcatt tgcagccatt ttaccagcga
    55081 cgatcgcttt atacgtcatt gctattatca attatatcgt aggtcgtgta gctgatgggc
    55141 aactgatcat tgatttggtg caaaaatata ttgcagaacc attcctagga ttatctcaag
    55201 ggttgggagc agtattgatc gtcacagtat ttgttcaagt attctggttc tttggtattc
    55261 atggaccaaa tgtattggca ccagtgctag agggaatctg gggacaagca caactaatca
    55321 atattgatat tttccaaaaa ggttataacg gaaaaactgg tacaccagcc gtgttagatg
    55381 caatcgaaga tggaaaagct tatatgtggg tccgcggatc gttcgatgct tttgcatggt
    55441 ttggcggatc aggcggaacg atcgtcttac tgatagctat cttgattttc tctaaacgtg
    55501 cagattatct aacagtagct aaattatcca ttgggcctgg tatcttcaat atcaacgaac
    55561 cagtgatgtt tggtctgcca atcgtattga atgcaatcat gtttatccca ttcttgattg
    55621 ctccagtagt agcaacagca attggatgga cagctactta tcttggccta gtcgctccgg
    55681 tatcgcagca agtcacatgg gtagtaccgc cgttcttatt gtcattctta gcaacaggag
    55741 cggattggcg ggcaccgatc gtaacattag tatgtatggt cgtgactttc ttgatttggg
    55801 caccatttgt catctctgca aacaaaatgg accctgccgc aggcgaacaa caataagatg
    55861 aacgtgagga gcgggacgct ttaagtttcg ttcctcataa tgaattttct aggaggaact
    55921 gaaatgatta caattgccag tgcaatgcaa caagatgaaa tcaaagaatt gttagaagga
    55981 tacaatgaag aaggaatgac ttttacattt aaagaaaaac aaggaatcaa gctgttcttt
    56041 gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag caaaggaact aatcaaagca
    56101 cagccttggg gaacagtcct ttattttcaa tcaacagctg tttagaaaat ggtttaaaaa
    56161 gataggatgg ttgagatgat aaaaaacggt ttatttttat gtagtattgg ttctttgatc
    56221 gtgttgtata gtttgaatcc acattcgcaa aggtatgatt tgtatagtat ggtcacaggt
    56281 ctttttctaa caggattagg aatctatttc ttcatgaagg ggaaaaaaag agaagaagca
    56341 aaggagaaaa ataactaatg gaaatcacga aaaaattaaa tattccagca tcgttttttt
    56401 acgataaagt tatggactct gtccttttcg atgtacgtaa agcgaccggc aaatcagtaa
    56461 ctagaaaaca gctgaaaaat ttggagtacg tcaaacaatt ctcgcaaaat aaccgggctc
    56521 gcataaaaat cgaagaagtc attgaaaacc agtcttataa gtttcgtaca tcgacaacga
    56581 agaatgaatt cgtcgttcat tatcaaatca aaccattgga cgattcgacc tgtgaggttc
    56641 attacacaga aaaaatggag tcttatggca tgcttcaaaa gttgaatgat atgcttttag
    56701 gaacaatctt gatgtatttt aaacggagac gtttcaaaaa aatgctagat atgatcgaag
    56761 caacgtatta aatcaatgaa acatggtact atttagttat tgaaaaatga agtaagctag
    56821 aaggaatttt tcataggaag atagatcttt cctgcgaatc ctcactagct tttttgtagg
    56881 aaggggcagc aaaatgacaa acaaagtaag agagctaggt gtatcttcga ttgaggaaat
    56941 gtttgattta gccacttatg catttaatgg ggcagataca cctgaaaggc gagaacgttt
    57001 tcgtacactt gcggagaatt cttggaatta cggatttttt gatgaggagg atcggctaac
    57061 aagtcaagtc atggcgaccc cttttccagt gaatttttac ggacaagagt acctaatggc
    57121 cggcattggc tatgttgctt cttatccaga agcaagggga caaggcggga tcaatcagat
    57181 catggaaaag atccttgaag attgccgtga tcgtaaagta agcttatctt atctggcgcc
    57241 attctcctat cccttctatc ggcgctatgg ctatgaacaa tcatttgata aaatcagtta
    57301 tcaactaaac agtcgtgatg tgccgtttat caaaaaaaaa tctggaaaaa taaagcgtat
    57361 ggattttgaa gatgcaaaag ctgcaatcag aagaatttat gaacagatgc cggaaaacca
    57421 acgagctggt ctgaagcgcg aagactggtg gtatacgtat aaattcaagc aaaaaggcaa
    57481 ttaccagttt gctttgcatt ttgatgataa tgaggaagca acgggatata tcgtttatca
    57541 gcttgcaacg tcttccttta ttattgcgga gtggggattt ttggatcatg atgctttttg
    57601 cagccttgtt cgcttcgtaa gttctcacaa tggtgctttt gaaacatttg aatattcatg
    57661 cggctacggc ggtaatgatc aaaattatct attagaaaat ccatttgctt ctgtaatgat
    57721 cacaccttat atgatggcga ggatcgtaga tatttctttg tttttggaaa attatccatt
    57781 taaaaagaga caagctgcat ttgccctaca aatagaagaa gatcattatg cgaaatggaa
    57841 tgaaggaatc gtggaagtaa caatcgaaaa tggaaaaaat catctctcaa aagtagaaca
    57901 aacaaaactc ccaatcgttc gaggaagtat ccaacatatc acgcagctat tattagggta
    57961 tcgtaaagtg gaagagttgg tattccaaga aagaatacag gtgacaaaag aatatgaaga
    58021 aacgttaagc tcaatcttgc cacagcaaca gccaattctt aatgactatt tttgataaaa
    58081 aacgggtgat gacaatattt ttgtcaccac ccttttgttt ttcatttggc atttgaagga
    58141 aaatgttaaa gtaaatcctc ttttttcgta aatttgaatt ttttgttttc ttttgtttta
    58201 gggttttctt ttttgtggtc ttggattgct tctttcgtaa tcttttcccg atttttttta
    58261 gatgaatcta agcgttctgc gtatactttt tcttttggct tatcattcat tggaaaggac
    58321 ctccctacat tatttcattc tacctctagt ttatcatctg ttaaggacta gtggaaacgt
    58381 tttgcctaac ttataagctg atttttttag cagataagct tgcaagtaaa aaacaaacaa
    58441 gctataattg atttattgaa attgttcctg tagctcagta ggatagagca atcgcctcct
    58501 aagcgatagg tcgctggttc aattccagtc aggaacgtat aaataacccc tgtatccttt
    58561 gcggtacagg ggtttgtttt gtatttgtgc caaaaatgtg ccaaactagt tatcattttt
    58621 atataaacta tcgaacacag tattgatttt ttctttatcc tgatcttcaa gctctcgaac
    58681 aatatgaaga taagtagaca ttgtagtttc taaactgcta tgtcctaaac gtttcgatac
    58741 gctaagtata tttactcctt gataaagtaa aatagatgca tgtgtatgcc ttagtccatg
    58801 caaagtaaat tgcttttcaa tccctaattc ttttaatttt ttgcgcaaat atttgctgac
    58861 ggcgtttgat gaaactaatc cattttttaa gttgaaaaaa acaaaattat gagggttttt
    58921 gatttcaaaa ttctcgaata attctttttg attgatttta aactttttta agagatcaat
    58981 cgtatgttta tcaatggaaa ttttcctatt tgaagtttcg ttttttgttt ttccccattc
    59041 atttaattta taattccatg ttttattaat agtgatgatt tgatcttcga aatcgatatc
    59101 ttcccatgtt aatcctagaa gttcagcaaa tcgcattcca gtggcaccag caactaacac
    59161 gagcatagga gaagagtact gcgcagaaag gttttcttct acgagtttca ttaaagattt
    59221 aaattgatca taatctaaat atttatcttc ttttggcttc aatgaatctt ttcctttgat
    59281 tactgctttt ctagttggat caaacggaat taatccttct tctacggcat ctttcaacga
    59341 cgctctaata tggttattaa acttaataac ggtagatttt acatggtctt tagcatactt
    59401 atttaaaaag cgttgatatc ccactctatc taaatcagaa atcaataccg ctggcatata
    59461 tttctttatg ttcattaacg tatcttcgta ttttctataa gtgataggag atactgttcc
    59521 ttctttataa agttgcatcc aatcctcaaa gtaatctgat aataatagat ttttgcgatc
    59581 ccctttaagg cctttgttca agttatattc taattcgttg gcggcatctt ttgcttctcc
    59641 ttttgtctta aatcctgatt ttcttatctt cgtgtatttt ccgtcatctt ttttgtatga
    59701 aatttcgtat tgccaactat ttccacgttt gactaatctt gccataattg aatcagctct
    59761 ctttctctga tacaatagga actataaaga agcccattgt ttgggtttta ttattttttg
    59821 cacatccttt ttgcttgccg gcgcaaggat gtgctatttg tcgttttaat ggtataattg
    59881 gatgtgtatt agatcatcca atcagaggtg tttattatga ttaaagtgat ttttaaaaga
    59941 ataactgaac ttccagagtt tagattattt gttgattatt ggtatgtctg gttttttatt
    60001 gttgcattgc tgataggtct agatgtcata ttagaaagaa aacttaagta agagttgagt
    60061 taataaattg aattcctaaa tgagatattt tgtagcccat attttgtttg ccgacatcac
    60121 ctaatactat ggagctaatc gttttttcta acagaccaaa atcagataat ctatttaagc
    60181 taccatctat tagctcttgt gaatagccag acatgctgat atttccagtg aaattttcgt
    60241 ttccacgact cattaagcat gctaacaatt gaatctcaat cgatgttaat tgattcagta
    60301 caagaagata atactcctcg ttatcccatg aagcgctgtt aattcgaagt aaacaattct
    60361 tataaagatt tttatacata tctattttat tttgtgcctt tgatttttct acctcgtcat
    60421 ttatcccttc gaatattcca atgagttggt ccttgttctc taagttgaaa acatcttctg
    60481 gaatttgatc gcgaatgttt tctaatgatt cgtttaaatt tttgtagaat ttctcgatgc
    60541 gtttgaaacg cttctcgttt ttagacccaa aataaagtga ctgcacaacg ccaccaatat
    60601 aaggaattgc tccaataccg ccttcaacta ttaattcaag atattctttt ttggttagtt
    60661 tttcattgct attatccata gttacctcct attttgtcat ttcctttaca agcattgtga
    60721 ctcctgatgg cactcttttg tataggtttt attattgtgc acaatctaac tcttagtggg
    60781 gcgtggttca tttatttttt acttacttta ctattaatca tatcccattg agcataggat
    60841 ttttctatgt tcgatcctct tgaactaatt tctcctgtaa aagttacttt atcgcccttt
    60901 gtaaaggtat cagcattgtt taagtccttc gcaaaaatta catatgaaac tttaggtgtt
    60961 gcagaaatat cattccaact tttatcatcg tactctttgt ctgctataat ggctattcta
    61021 gtagtcattg attcgataac agttccttca aaagtatatg ttttaccact aatatattta
    61081 gtaaaagctg tattttgctc agtagctgga gtaatagaat aataactatc taagaaagca
    61141 ccccagtcat gattatttgt ctctaaaaat tcttcaaatt gagttaggct tttattgctg
    61201 ctgttagatg tagtagtttg agtatcagat gatgagcttt cctgtttgtt attgtcattt
    61261 gcctcgtttg atgaggaaag cgagaaacct acggtagtaa aaataatccc gataataagg
    61321 agaatcatag gtgttttctt atttgttttt ttgataaatc ttacaattaa catgattaaa
    61381 cctataataa ataaaacaat accagtaaaa ccaaatatac cgtccattaa tagatcctct
    61441 ttctcttttg atattgaata ttataaacca agcaattctt ttttcttaaa atcgaattct
    61501 tcttgattaa taatcccttc atctaacaac tctttatact ttttaatttc atctgcactt
    61561 gaaatttgtg tttctttgga attattatct aatatagatt gttgcttctc tatatatttt
    61621 tttaaatttt ccatttcatt ccaatatttt tttgtaaaca taacggtgtt ttcatctttc
    61681 gtggccgcta atatacctcc gcgactttcg tttccgccta gcaaggtaaa ttgtatataa
    61741 ccattactca tacctggttt ttttaattgt acggctgata tatttttaaa ggggatagtt
    61801 ttttctcctt taataccttg attaactaaa tttataaatc cttttctaat aattgaaata
    61861 gaatcatcag ttactttaat caaggttttt cctggagatt ttattaaaat ctctttttcc
    61921 attttttctt cctcatttct gtgatatgat tttttttgta gaagctcata tatgaggaaa
    61981 gagcccgtgt tgcagcacgg gcttttttaa tgttttgctg aaatcggttt ttttaataat
    62041 tcttgatgta ttcatacata ttgccttgcg taagaatatt ttttcttaaa atagcattgg
    62101 cagacagcat aacaagtgta tctgagctta ttctgaatag aataatactc cataaatttt
    62161 tcgagattaa actgagattc atcagtcagt tcgttctcaa tatagatatt taataaaatt
    62221 agaatagcta ttttatctgc ttctgtttca aattttgagt gaaaagttgt agaagtatcg
    62281 tacaaaactg aaaattcaaa atgagaggcg ctgaaatgtg caagttcatg agataaatga
    62341 aaggcttctg cagtttcacc gtatagattt tcattcaaaa aaatgattct gggttttgga
    62401 tagtagaaac caggctcttt catctccata taaactactt tcaaattgta ttcgctcaac
    62461 atttctttca atttcaaata catacaaacc atcactccaa ctattcattt tcctctaaag
    62521 ctttagcaat tgcaatcgct ttacgcattg tctccttaga tatttctttt ccatcaaaag
    62581 aaaaaacagt atcgtcttct gataaatcca catgtttagg ggtttctctt tcttctctac
    62641 ctagaaggta gtctacagag acatcgaaat agtcagcaat ccgacttaat tcatctgaat
    62701 taggagtatt atttttccac tttgctagat aaccgttaga atatccaaaa ttaatttcta
    62761 actgccgtat agatatcttt ttcctcttcg ccaagtcttt tattatttca taggtattca
    62821 tcgataaaac aacctttcta aatgtttaca aaaaaagttt agaaaaatac gcagaaataa
    62881 tttgactaat tctgagtaat gagctatact atatcttgta aacagattta acaactaaaa
    62941 agacaacaaa aaacactatt gatatgtaaa tgcagaccgc caagaaagct ttaaaatcaa
    63001 tgtttttatg tcttatttaa ttatgctctt agtatagagt attactcaga tactgtcaat
    63061 tgaatttaga aaaaagttgt taaatttgtt tacgaatata aaagaaagga atgagaaata
    63121 tgaacacacc gcaaattttt aatttcgaac aaaacgaagt tcggacaatt ttagtaaatg
    63181 atgaaccata ttttgtaggc aaagacgttg cgagtgtttt gggttattca aacactaaag
    63241 atgctttgtc gcggcatgta gatttggaag ataagatggg gtcgcgaatc acgacctcag
    63301 gtcaatcaag agagatgaca atcatcaacg aatctggttt gtacagttta atcttaaaat
    63361 caaaacttcc ctctgccaaa aaatttaaac gttgggtaac aagtgaagtg ttgccagcaa
    63421 ttagaaaaca tggaggttat ctaactccag aaaaagtaga agaagctttg cttaatccag
    63481 atacaatcat tcaattagca actcaactaa aagaagaaag aactggaaga ttaatcgcag
    63541 aacaaaagat tgcagagtac gaacctaaaa tctcctattt agatagcata ttatcttcta
    63601 cagattcagt aacaattagt cagattgcag cagattatgg gatgtctcca caacagatga
    63661 ataaattact tcataaacta ggtattcaga aaaaagtcgg taaccaatgg ttattgtgca
    63721 aaaaacacat gaaccaagga tacacaaaat ctcatacaac tgagatcccg aaagccgatg
    63781 gtggcactaa aattgtaatg aataccaaat ggacacagaa agggcgtcta tttatctacg
    63841 aattactaaa aaaagaagga tattaccctc aaatggattt agaggaaatt ggttagaaag
    63901 gagttttagt atgactgaca ttgcagaaat cactcaacga gatagagaaa aaatcaaaga
    63961 atatgtcgaa agttcaaagt tcttaactta caccatgctt gctgaaagat ttggaattag
    64021 caaaagctac ttatctttaa ttttaaacgg taaaaagact tctgcagaag caaacagaat
    64081 tatagattcg attatcacta tgtacgaatt gtaaagggag gaacagctaa tgcaatatct
    64141 agaagcaaaa atcccaattc cagaaggcta tgtaattatc tcccaagtgg attatgagga
    64201 gttaaaaaaa gctgatgata ctggtagatg gatgacgttg ccagaagtgc tagaacggat
    64261 taacagaaaa tatgattggt ttacttctag agttttaaag aacccaagat atagaaatat
    64321 tatcgatatc gagaaaaaca aaaatggatt tgtctattat ccagttgaag gaagagacac
    64381 atatctattt ttaagaagta aaacacttga atttttagaa acaaattttt cggaaatctt
    64441 aaggaggcaa gcggatggga aaatttaata gagcgctagt gttcagcgca ccgctaatca
    64501 tctacgcttt aggactttgg ggaagcaggc aagcgttgat aggaacgatt gtttacatgg
    64561 tttggatttt tatggggctt gatgaagctg aagctgagta cagagcgaaa aagccaaccg
    64621 agggggctga ctaaaaatga atagaaaaga gaaactagaa tgtatattac tattactcag
    64681 tttaattctt tcactaattt ctctattggg aagtttgtat ttttgatatc aacaattttg
    64741 tggatttggg ttctatttag agtaaatatc atttttgttt ggtgattaag gagtttatta
    64801 aaattaattt tccctacttc aaatgcaagg taacaagata tagcactttt aggaggaata
    64861 tttagcggta cagcttcaga gtatattcta ttatcatcga ctgatcctcc tttagcaatt
    64921 aatttcttaa atggagtagc tgaccatctg cggtttaagt tagcggatga aagttctaaa
    64981 ttaactaaca tagctggttc tgttgaatag ttagaaatga ttacttttgt ataaatcata
    65041 tctgcagcga agtaagaagc attaagttca acttctagtt gagggcgcat tccttttatt
    65101 tttatccctg ttaaaaacgt actcaatata aaacttatta ttgacatcca ttgaaaaatt
    65161 gttaagtgaa aaaaattcaa aataaacacc accagttttt aactaaatta taccaaaaag
    65221 gagagaagaa ataatgcaag aattagtaat tttgaaaaat aaagaagctg tgactacgag
    65281 cttacaagtg gcagaaagct tcgagaaaaa acatcaacat gttttaagag atattgatgc
    65341 actaaaaaaa gatgtgtcca attttggaca gatgtttgta gaaggtaatg aaccagattc
    65401 atatggcaga aatcgacgag ttttcttcat tagtagagat ggatttttct tgctggctat
    65461 ggggtttaca ggaaagaaag ctatctactt caaacaaaaa tacattgaag cattcaacga
    65521 aatggaagat gttattcgca agaatactgt tcctcaaaca attgaagata tgatgatcta
    65581 tcaattagaa gaaatgaaag atgttaaaaa agatgtttcc atgcttaaag atactatgcg
    65641 aattagcgga caacaagagt ttgaaattaa gcaaaaagga aatatgaaag ttatgggagt
    65701 tctaggggga aaagaaagcc gagcttatga agaaatcagc aaaaaagtat tctcaaaatt
    65761 ttggtctgaa tttaaacgta ccttttcaat cccaagatat ggcgagttac ctcgtaagag
    65821 attcgatgat gctgtttcat ttattgaaat gtggttgcca gaaactgcga tccgtatgga
    65881 aattgatcaa ctgaacagac aacagagact ttttggtgat gacaatgaat agagctgaag
    65941 cgctaagaat agggacggca attgctaatt gctggtggaa atactataaa ccaatcatcc
    66001 taagccaaca acatattgac aagcaaaaag catggcaaca aataaaaaag tgactccgcc
    66061 ggcaagcaaa gagtcacaaa gaaaacacat cataaggaga ttttagcata tggaaaaaga
    66121 actttccact ctagatcaat atttgattga tcctgattgg ggcaagccga aaattgagga
    66181 aacaagtggt cgaaaaatca gacgaaatct tttgacgaat gaagaactag cttgggatca
    66241 agatgattta ggcaaccatg taactatttg ggatcatgtt tatcttatcc atctatcgaa
    66301 gcattcgaat aaacctgaat atatttacgt catcgaagat ggcttgattg atgcgctaga
    66361 agagtacgac agagataact tgattgatat ctcttattac ggaccaggta agaaatacat
    66421 tgctgaaatg gaggcagaat ttgatgagtg aaatcaaagg gacaacgaac tttgaaaaac
    66481 tttttagtcg taagttaaat aaaattctca agaaaaaagg aaattttgat tatttatctt
    66541 gggctcacgc gtgggagatt atgaaaaaga atgatccaca ggcaacggta actattaatg
    66601 agtacaaaca ctacagggtt gtttctggaa ctcatcaaga ctttcttgtt gaggaatata
    66661 aaccttttct tatggatgaa actgggactt atgtatctgt ctcagtaacg gttaaaggac
    66721 acacggaaac cgaattattt cctgttttag attatcgaaa ccaaccagtt gttaaaccaa
    66781 atgcaatgca aatcaataac tcattgaagc gatgctttgt gaaagcattg gctctacacg
    66841 gactgggatt atatgtattt caaggggaag atattccaac accacctaga atcgatacaa
    66901 agaaattaaa catgctagag acgattctag aagctttcaa tgagcagatg ggtaaagata
    66961 tgaccaaaac cttaatcgaa tatgttaatg agcagacaga taaattaggg ctcttagctg
    67021 ataacgttga aactattgaa cagttaagct atgagcaatg tgccttgatg gagcgagcaa
    67081 tagcagctaa gagaaaagaa ttagataaga agtgatatga gtgtttaaac cattaatcga
    67141 ttcatattca gcggttctga aaaagttcaa aggaaaagac ataagcgcaa ccatcaatga
    67201 ggaagtgaac attgatcgac taaagacgat gtatgacggc tacgatggtg atcgagtcat
    67261 tgaaattcgt tttattgatc ctagacgttt caccgtacag caacgaaact tcatctatgc
    67321 gctgataggc gatattttta tcgatacagg catgccaacg gacttctgga aggaattctt
    67381 ctactttcgt tttgaaggtg tcacagggcg caaaataagc ctgaaagacg aatcgaatac
    67441 aactgtgagt gatgccaatg tcttagcaaa tatcatctta gatttcatct ttgaacatca
    67501 tattcctttc aaagaaggct atgagatttt acctgcgaat caagaatatt acttctacaa
    67561 atgcatcaca aaaagagtct gctgcatctg tggcaaaaca ggagctgaca tcgatcactt
    67621 tgacaaagcg ctaggaagac gaaagcgcaa agaagttgat catgcagagt acacatttgc
    67681 agcactctgc agaatccatc acacagagaa gcacaaaata ggtgtgatta atttcaaaaa
    67741 taaatatcaa atcaaaggga tcaaattaaa ccagaaaaca atcaaaaagt taaatattgg
    67801 agggtaaaaa tgacagaaca tcgaagttat tatgcgatta taccagccaa cgtaaggtac
    67861 gacaaaagac ttaaaccaaa tactaagttg ttatacggag agataacggc cttgtgtaat
    67921 gaaagaggct tttgttgggc aggcaatgag tactttgcag atttatatgg tgtgaataaa
    67981 gagaccatat cgcgatgggt aagtgatttg attaagtttg gatacttgaa tcgggaaatc
    68041 atttacaaag agggtaccaa tcaaataatc aataggtacc tacgaattaa tcaataccct
    68101 attgacgaaa aacgcaatac ccctattgac gaaaaagtca aagataataa tacatctatt
    68161 aataatacat ttaataatac aaaagaatat ataagagagt taccgccttc gaaaaaatcg
    68221 aaggctaagc ccgtccgtca taaatacgga gagtataaaa atgttctttt gtcagatgag
    68281 cagatggaga aactcaaaac agaattccct aatgattacc aagagcgaat cgaacgactg
    68341 tcagagtatt gtgaatcatc tggtaagact tataaaaact atttggcaac tattcgaagt
    68401 tgggcaagga aagaaaaaag tgaacctaag aacgcaagca gtggatacaa gcgcacagga
    68461 agacgagaga agcttcctga atgggcaatc gaccaagaag cctatctcaa gaaaaaagcg
    68521 ctagaacgag ctaatagaca atcaaaagca ccattctaag aggtggaaaa atgaagatcg
    68581 attatctaga actaattaat gaaatagcga attataaaaa gggcgaggaa ttagacgtcc
    68641 tgagagacgt atatgatcaa ctcgaagaag ctggaattga aggaattaag aatgatcgtt
    68701 cgagttggag taagctcaga tactatttcg cactttatat cgatacaaca caattaagaa
    68761 atttagcata taccaaatta ctatttgttg attgtgttaa aggcttgcaa aaacatctta
    68821 atgaacttga gcaggtgtaa tcagatggac ctaaagacat ttacagcaca gattgaacta
    68881 atgcatcaag aagctttaag acaaagtgta tcgtacgaag acaagtggct caacacgttc
    68941 catggcggac gtgagagcgc acttgatcaa gtactcaaat tactgaaagg agaatgtcgg
    69001 gatggataag aaagcggcaa tgcagcgaat tatcgaattg acttattcag aagattggca
    69061 aaatgacaaa gaagctgctt cagaagtgat gaggcttgga agagcgatgt gggcagacaa
    69121 gagcaacaag ccaagaccac gaaaaatcgc aatttggcac ggtgacaaac ttctagtgat
    69181 agggacagct gaacagttag caagtctcac aggcttgcac gagaaaatcg tgaggaaaag
    69241 agcaaggtgt ggctacacag acgctaagaa gagaacgttt agatacgtgg aggaatcatc
    69301 atgacaacag aagaagtgat tcaaatgcgt attcgaagcc ttcagcgtga gattgacgat
    69361 ctggaacgaa caaaggcagt gatggtcaat gaaacggcga gaaaggcaat cgatttgcac
    69421 atagagaatt taagaaggga aatccatcga ttggaggaat gagcgtggat aagaaagcag
    69481 caatgaaaag aattgctgaa ttaaccaagt cagaatcttg gcaagaagac aaagaaatag
    69541 ttgcagaagt ccaaaagctc ggtaaatcaa tgtggactga aaagcccaaa cggagaacgc
    69601 cgagaaaaat tgcaatctgg catgatgacc gaattttagt aacaggtact gctgaacagt
    69661 tatctgaaat tactggatta agcaaaaaca ttatctggga tagagctagg agcttatgga
    69721 ttgattcaaa aggacgacag tttaggtatg tggaggagag ataatggatc tcattacaca
    69781 atacagtgac atcatcctca agaaaatcat gatgaagatt cagaaagata aaaaatcaaa
    69841 agaacgagct gaattagtta agttagaaat ggctgaaaca ggagcaggag tgcgaagtag
    69901 caggcattgg aaagcagcag caaacattga attttattac aacgaaattc aaaaagggtt
    69961 cgatcagatg cgtgagctgg atcggcaaac aaattggagc aagaaacttc atcaagatcg
    70021 tttcaaattt gtagagaagt ataaagaaat attagaagag tatttgagga ggacagcaaa
    70081 tgataaaaaa actcgttcaa ttcagcatgg atttatatga tatcgaatca ggagcaacac
    70141 tatctgtgga atcggaccat ctaatcataa attttggtgg aaagcgccag attattttgt
    70201 gggtagttga tgatgtactg tttccagaaa ttgttcatga tttcgaagaa tcaaaagcgg
    70261 ttgagtttga aatagtgaaa aaagtaatgg aattgattga aaaatacgag gaggacagcg
    70321 aatgataccg aagtttagag cgtgggataa acgaaagaac gtaatgagag atgtagccgt
    70381 cttgcatttt actaaaaacg gcaaaacaaa ctttattgaa tattggataa atcctaccga
    70441 attgaaatca tatcacgtgc gaaacatcga cctcatgcaa tccacaggaa tgaaagataa
    70501 gaatggtgtg gatatatttg aaggggatat agtattagtc agcgtgcgaa atggcttcga
    70561 ttacttagat aataaagtct gtattgtcaa aaattcaata gattattccg gattagtttg
    70621 tgccactgtt gatgaagact tagagtatca aatttttaac acagagctgt ttgaagaata
    70681 cacgtacgaa gtcaccggaa atatatacga gaatagcgag ttattggagg aacagcgatg
    70741 aataaacagg aagcaatcaa aaagttagaa agtattaaag cgataggaaa tgatgcaata
    70801 gctgcttgct ataacgagag tataaattca ggtattacgt taatgaaaaa aatagacgaa
    70861 ccgcagaaac cagttattcc acaactcgtg gccggttggc ttgagaaatc tacggaccct
    70921 tttacaaaag ctgaaaaaat agcgtattta atcaaatcta aagatggtga ttcatattat
    70981 ttctgtgatt ggtttgtacg agatggcata gtgacgcaag agcaaggaga agaattactc
    71041 gcttgggcaa caagacaatc atatgaaaca ctattgagcc tatacaacgg ctacgaggtt
    71101 gagaaagagc cgttatgggc aataaagaat gccgatggaa actatcttac taaatgtgct
    71161 ttatggggaa aagatggagt aaattatagt tttgaatgca atccatctca tcgattgctt
    71221 ttcactgata aagcaacagc ggatgctgca gcattgttgg tgaatggaac agtggaagag
    71281 gtggtagaaa gatgaaacta aaagacggat tttacgctag cagtcacggt atcggcggtt
    71341 taatgctaga tatgccgaca aagaacccta aaacacgcaa gaaatcaaaa ttcaaagtcg
    71401 gtgacatggt ccgctgtgaa gcagaagagt tcgtttatcc gttcagagga tacgttaaaa
    71461 agatactgtc aaactcagca atcattcgta ttgaaaatac aatgaagtgt gatcgttcga
    71521 ctgcaaaaca caagcattat ttagctgtag cgagattagt tgacatggaa gtaatcaaga
    71581 gcaaataaaa aaagccggat cgctccgact gattcaataa atccaacaca tttattatat
    71641 cacataaagg agcggtttga cttgatgcaa ttgttacgag aggtagattt caaacagaca
    71701 agatgtaatg cgagagatgt gctgaagaac tttcggcgtt tggagcggat ggcaggtcgc
    71761 tctttgatag atattaagtc gccgattata acggatatgc cgaaggcacc gaagcatggc
    71821 aataaggcag aagacgcgat tattcagatg atggatatag aaacggagag agatgcgatt
    71881 ctagcggctt tgatggctct tagtctgatt agtcgtcaga tactctacta cagcttctgt
    71941 gtgccagata gcttctcaaa ctacagaatt agccgtgaag tgggttattc agaaagaagt
    72001 atacaacgga tgaagtcgga agctctaata gagtttgcag aagcatataa acacggaaaa
    72061 ataattgctt ataaataatt tggcggtttt ttggcggaat gatggcggtt tttagacatt
    72121 taccagtgtt attatggtag tgtcgaaaga ttaggaaaca ggatcgacaa aataaacgaa
    72181 gggaggcaat ctccctcatc gttttaaact tcttcttaga tagacagcaa ctaattatga
    72241 agaaaaggat gtgaattcaa ctcctcctaa attcttcatc ggttgctgtt tattttgatt
    72301 agacagcagc actcacaaaa ctaaacatgt tcttgtcgtc tttcaactgc tgctgtctat
    72361 taatttatgt attggaggga agaaaaatga aattatcagt agaaggcact ccagaagaaa
    72421 tacaagaatt gctccaagct attggtagta gcaaggagca aaaggtaagt gtttctaatt
    72481 cagatattga cggtatttgc aattgtttaa acagagaaaa aatatctaat ccttactaag
    72541 agaaaatatg ttcgttaatg aatttccaat tttcctctgt agctctacct tgataatttt
    72601 tttgcagttc acagatgaat aaggaatcgt tattatcaag tactgtattt aatttttcac
    72661 acatttgatt tggattcaaa ttagttttaa ctaaccaaaa agatttttgt agctttatat
    72721 atgccccaaa ttcttttatt atttcaaata ctttatcata cttttgccct gggttattaa
    72781 gatcataact aatcatgtag ggtttataca tgttgtcttg cacatccttg ttttatattt
    72841 cagcggacca ctcgctgata aataaaatta tacgcttagt atttattttc acaatattaa
    72901 tttatcgtaa taacttttga gtaaatagaa caaaaaacct gcactagttt ccgctagtac
    72961 aggcagtgac tatatcggtt gataatctag catatattaa aaataattgc aagaaaagaa
    73021 atttatagtg gtttatacca aattatcaaa gcaatgttat tttgttgctg tctattgttt
    73081 tttaattatt cacacgataa ctaaaggtgg gtgaagagaa atgattccat taataatttc
    73141 aatttttgcg ctctgtctta atgtctatat gattggattt aaaaatgggc aaaataaaaa
    73201 atagtagcag ccaagaataa ttttatagtg tcactgtggc ggaaagggta gacgctaagc
    73261 atgtgtgcta ggtcaatgct tcggcaacca tgcaaggttc gattccttgc cagcgacatt
    73321 aaatgcctat gacggttacg actaccgaaa aaagatcgtt aagaagctat acggtgctac
    73381 gtacggcaat gtagtaagtg tgctatctgt acaccaccaa gcttcggtca ctgtggcgga
    73441 agtagaagac gcagcggtaa atggcgagta gcctcgtgag agcctggtaa gttctcgtga
    73501 gtggtgcaat cccactccag cgactttaag caaccgaggc atcggcggtt taaaaatata
    73561 ggggtgcgca atttcgtacg cgttttgtgc atcgtgcaag ttactattac atattagatc
    73621 actctttgag tggtcttttt atttttgcac aaaggaggaa acaacaatgt atagaccaca
    73681 atacttagaa cagaagtatg aagtaatcac tgttcataaa ggtaatggcg aaaaagtata
    73741 tgagtataga agaccaataa agagcgatac atataaacga aaggaaaaca atgaagttat
    73801 tccattgtat ggcaaaagaa tagctaagca ttaaataaga ttgcgaaagg agacggaaca
    73861 tgaccgagga attctataga tggctattac agttgacaag agaagatcgt ttggttaagt
    73921 tctatcagtc tcctaaatgg cgcaggctta gagagaaagc gatgaaacga gatcactatg
    73981 aatgccaaga gtgtagaaga ctaggtaagt atcatagagt agagaacgtt catcatataa
    74041 aggaagtcaa ggatagacct gacttagctt tagatttaga taatcttatt tgtttatgtg
    74101 ttgaacatca taatgaagtt catggcagat atcttacagc attagataaa caagagaaga
    74161 agatagaaag ctttgctaac ttcgatgcaa gtgaaaggtg gtaagtgcat gatcatcaat
    74221 gataatggca gagagtatga tacagaaaag attgaagagt attcatctta tactcaggga
    74281 ttaattaaac gtttgatata cgttcgctat gtaggtatta gggatctgtt atcagataac
    74341 tgttgtagta aatacaaagt gaatcaagta agagaagcgt tgaataaaga taataacgtc
    74401 gaaagaataa aaaatgtttt tggatatagt attgaagaga ttaattatta cattgacttc
    74461 gctgaagctt tcattccgat ggtgagataa ccccccctta aaataaatcg caaatttttt
    74521 gggggtgatg aaacggaggg ggctgtcagg aaaagagatt ttttcgaact ttatcatgaa
    74581 aggagggcta aaatgtttaa aaacgaattg tctcaaaatc gctacagaga aaaattacgc
    74641 cgctctttaa taagccaatt ggaaagtcag aaaacaaata ttgagccatt cttagataat
    74701 gttgatcgtt atatcagttt atgggaaacg gcgatatcac tggaagaaga tatatccgag
    74761 aacggcatta gactggagaa tggtaaaaag aatgaatcag tagcgttgct tgtttctgtc
    74821 aacaaacaaa tgggattgat gttggataaa cttgccatta ctcctgaatt ggtaggtgaa
    74881 gcaaatgaat caattcctga gttataagca tattgaaaat tggttcaaag ctatagaaga
    74941 aggcactatc aaggtatgca aagagcaatt attgctaaaa aattatctag aagaaagagt
    75001 ctttactaga gaagatattt acttcgataa gcagatggta gaggattcaa tcaatatacc
    75061 agcacaatac tttccattcg aattaattcc gtgggaaaaa tttctacaat gttttattta
    75121 tggtgttcga tggaaaaaag ataaaacact agtgttcaat agatatcttt cattaatggg
    75181 acgtggtaat ggtaaaactg gttttgcttc ttggaacaac ttctttctac taaccgctaa
    75241 acacggtatt aaaaattatg atattgatat ctatgccaat aatgaaagcc aagcaaagac
    75301 tagttttgat gatgtattta aagtaattaa agatcatcct gatttagata aaaaagtatt
    75361 taaagctacg aaggaagtta ttcaaaatat cgctacaaac agcaaacttc gttataacac
    75421 ggcaaatgct agaacaaaag atgggaagcg accaggtgca aaccgctttg atgaaattca
    75481 cgaaaatgaa gattattcaa tgataaatgt ggctacttct ggtggtggta aaattcgaga
    75541 ttatagagaa ttttatgata caactaatgg tcatgttcgt ggtggtccgc ttgatgacat
    75601 tatagaagaa tcaaaaatga ttctttctgg agaacttgga attgacaagg atggagcaga
    75661 attttctagt ttgtttccat ttatttgtcg cttggataac gataatgaag ttgatgatcc
    75721 cgacatgtgg gaaaaagctt gtccaactat taattacaat gcagatttaa aacggaaaat
    75781 gtttcaagaa tactctcaaa tgcaacgtaa tgctggttta agacttacgt tcatgaccaa
    75841 acgaatgaac agacctatgg aagatacacg atttgctgtt gcttcatatg atgatgttct
    75901 gcatacgaaa gaaaaagaat ttcctgaaaa aatggatgaa gtgataggaa cagtcgattt
    75961 tgctgataga cgagattttg ccagcgttgg gttgctagga aaatacgata aagatgttta
    76021 ttttacacaa catactttta tccacgaatc agcccttcga ttacaaaaca tcaaacgaga
    76081 ggttatagat atttctatag atcaaggaaa atcacagatc gttcatggaa aaaatataga
    76141 agctgattat attgtaggtt ggtttcttga aatgagtaat aaatattata ttaaaaaaat
    76201 cgctatggat atgtaccgtg caaaaatatt gaagcccgct ttagaagaag caggttttac
    76261 tgtggaaatt gttcgaagcg gatctgttac acatggtatg ttaaaagatc tggttgatga
    76321 cctttttatt aatcaacgtt tattttttgg tgacgatgcg attatgcgtt ggtattgcat
    76381 gaatgtatat gaagagcata tttctaatgg aaatatacgc tatgaaaaaa tagaacctga
    76441 aactagaaaa acggatggct ttttttcatt ccttcatggt ttgaattttt tagatgatat
    76501 ttatgattct gctcctgtaa cagtcacaaa tagctcagta gaaaatacag gaactggatt
    76561 tactcctcta gtattctaac ttgaaaggag gtgagaaagt ggggattttt caaaaggcgg
    76621 taggatactt cacaaaaaaa gcaacggttc ctttagaaga atacttttgt aaattgcaag
    76681 ttgattttgt gtatcgaaaa tttgcgattg aaacttgtat tgatttgatt gcaaatgcga
    76741 tgagtaaagc ggaattcaag tcatatgaag atggaaaaaa taaaaagaat gatctttact
    76801 ataggctgaa tgtagctcct aataagaaaa ataatgcaac agaatttaga aaaaaactga
    76861 tcaggagatt aatattctac aatgaagtat tgatcgtttc tccgtctaat aattctagcg
    76921 aaatatttat tgcggatagt tgggatgtca cagaatatgc attgaaagat gatgtgtttt
    76981 ctcaagtgca aattaacaac atagtccttg atagagaatt tctagaaagt gatgttatct
    77041 atataaaata cgcagatcaa caaattaggc aactagtcga tgcgtattat caagcgtatg
    77101 ggaaactcat ttctagtgcc atgaatgttt acaagcgctc taacgctcgt agatacgtac
    77161 tgaaagggaa tttattccga ccgcaagaca atacaacaca agatcaaatc aataaaatga
    77221 tgacatcaca atttaaggct tttatggaag ctgataatgc aggtgcggta tttcaattac
    77281 aaaatgagta cacattagaa gatttcagcg gaaacttcca aagcaattca agagatataa
    77341 aaaacttaat agacgacatc tttgagatga cagcagcagc gtttcacgtt ccgaaaaacc
    77401 tactaaaggg agacatgagt gggttatcgg atcaagtgga cgctttttta atgttcgaaa
    77461 tcataccgat tgctgaactt attcaggatg cgtttaacgc tagtctctat gaagtagaag
    77521 aatacttgtc agggaatttt gtacgtgtgg atacaactat gatcaagatt actagcttca
    77581 aagatttggt tgacgctatt gatgtaggca ttagaaatgg agtatttaca atcaacgaag
    77641 gaagagaacg cgttggaaat gatcgctctg ataaggcgat ggcagatgaa atatttataa
    77701 ctaaaaacaa tcaacaagta tcgaaaggag gtgaggcgaa tgacgacaat gaaaacattt
    77761 ctagcagtaa agaatgaagg cgcagtaccg caaattttta ttcagggatt tattggttct
    77821 agttggttct ttgaagggaa tactgacaag ggaatcaaaa atattttgga tagtctaggt
    77881 gatcaagaag aaattgaagt agtaattaat tcaaacggtg gagacgtatt tcaagggatt
    77941 gctattggga acttacttaa gtcaaataaa gcaaaagtta acgttgtgat taacggctta
    78001 gccgctagtg ctgcttcaat tatcgcaatg gctggcgata ctataaaaat ctacaacaat
    78061 gcacaattga tgattcaccg cgcttccaca tacggagaag gaaatgtcga tgacttccgt
    78121 acgattgctg accaactgga atcaattgat aaatcggtaa aggcttcata taaaacacga
    78181 ttcaatggca cagatgaagc attgcaagaa cttcttgaaa aagaatcgtt tatggatgca
    78241 gaaacagctt tgagttatgg attggtcgat gaaattatcg atgcagaaaa tagctcaggt
    78301 actgaagcta aaaaagaaca aagcgttgaa gaaattttga atgacgttaa agaaaaaaga
    78361 gcagaaaaaa ttgctgcatt tacagcagca ttaaataaaa catttggaca aggagatgta
    78421 aaataatgac agttaaaaat ttaaaaggtg taacagctgc aagcgaccaa ttgatgaaag
    78481 cttttaaaga tggtaacgaa gaatctttta gcgcagctat ggtaagctta tctaaggaaa
    78541 ttcaggataa aattttagaa gaagcaacag caaaaaatca agatcaatta gtattaatga
    78601 accgtggtca gcgtgtatta actacacaag aaacaaaatt ctataacgaa gtagtgaaaa
    78661 acgaaggttt tgcaggggtc gaagaattag tgccagctac tgtatttgaa cgtgtatttg
    78721 aagatttaga acaatctcat ccactattgc aaaaaattac ttttgttaac acaactggtg
    78781 taacagaatg gattgtgtca cgtggagtca atccagcatg gtggggtaaa ctttgcgaag
    78841 ctgttaaaaa agttttagat aatggctttg atgtaattaa catgaagcag ttcaagctat
    78901 caggttatat tcctgtatgt aaggcaatgc ttgatttagg tccagtatgg ttagatcgtt
    78961 atgtccgtac tgttttagta gaatcattga gaattgcatt agaacaagca attgttgatg
    79021 gtactggtaa agatatgcca gtcggaatga tgcgtgacat gagcaaacaa actagcggag
    79081 aatatgctga aaaaaaagca gaacctatta cagctttaga tgctgtaact atgggcggtt
    79141 tgatggcgcg actatcaaaa ttcaatatcg aaggtgtgaa tgatccgatt tatcgtaatg
    79201 tgaatccttc tgatgtggtc ctaattgtga atccaactga ttactggtct aaagtattcc
    79261 cagctaagac tgtactaact gctaatggag aatacgtaca agtattgcca gtaccagttt
    79321 cagatttgca gtcaacggct gtgccagaag gaaaagcagt tattggggta gcctcagatt
    79381 acttcatggg tgtaggatct acactaaaaa ttgaagcttc agatgaatac cattttgttg
    79441 aagacgaacg catttatcta gctaaacaat atgcaaacgg tcaacctaaa cgtaacgata
    79501 gtttcattgt attagatatt agcgctttgg gaactactac tacaactaca aaaccaacaa
    79561 ccacaacaac tacaacacaa gcgtaggtga tcagaatgaa gtatattctt tgtcagccgg
    79621 caatcaatcg gtttaaatgg gagcttgaag tttgtttaac taatctgaag aaactaggaa
    79681 tcaaagatat cgtattgctt ttcagcagac acgatgatca gattcctatt ttttttgaga
    79741 aggaatatgg tgttgaagtt catgtgtacg atgatctgcg ggacgacaaa gagtatattc
    79801 cttcgattaa accatattta tggtggaaat atttagaaga agatcattcg cgtgaggacg
    79861 accgatattt ctatatcgat tcggatgtca ttttcaataa aagaattaat ttgcgcaaat
    79921 tgccttctaa agatgatgtt tggtattgta gcgactgctg tagttatcta agtcttgatt
    79981 atattagaag ctgtgaaaac ggagaaaata ttctaaaaga tatggcaaac attgtaaatg
    80041 ttacagtaga atctttggaa actataaaca ctaattcagg aggcgcacag tgggttatta
    80101 accgccctaa agcgaattat tggaaaaagg tttatctgga ttctaatcgg ctatatcgct
    80161 accttagagg gcaaaaaaca aatatacaaa tctggacagc cgagatgtgg gcacagcttt
    80221 ggaacatgat gtatttcaat attggtccta aagttcacga ggaattagac ttttgttttg
    80281 ctactgatcc aatagaaaaa gttaaagaag taaaaatctt acacaatgct ggagtaacaa
    80341 caaacgatga agatttattt ttcaaaggga gatacgtgac ttctacgcct tttgatgaag
    80401 atttatcatt tgtaaacaag aaaaaatgct cttacgcata tgttaaagca attaaggcgg
    80461 tggttagatg acgcctgaac aagtgactga agaattgcta atagctgtga aggataatat
    80521 ttacgttacc tggaacgaag aagatgagtc aattaaaaag atgatagcta aaaatgctgt
    80581 ttatcttcaa agtaaagtga gtacaacact ttctttttct cctgaaagct tagaatacgg
    80641 attgctaatc gaaagatgta gatacgactg gaatcgtgct ttagatgagt ttgaacaaaa
    80701 tttcgctagt gagttattag gtttcattca acattatgcg ctacaagaat atattgcagg
    80761 tgatgtgaat ggcgaataat cgtagactcg aagaaacatt caacgatggt tggttaaaga
    80821 ttttgacgca aactaccaaa agaaatgaac taggaaaaaa gattggtgta gaagatacag
    80881 aaatcacttc tttaaaattt agaaatcttt ccatgagaga tagtgatata acagctatgg
    80941 atgcgatggg atcgaaatta actaagaaag taaagactcc atttcatcca atcgccaaga
    81001 aatttaataa agatcaatat tttatcgtaa tcgatagtat gcgttacaac gttatctatg
    81061 ccgattacga taatttttat atctattttt atcttgaaag tgtgggtgaa tatggtgatt
    81121 gataattcta aagaaaaaga acgtttaaat aagcaaattt ctgctatcaa aacttcctta
    81181 gaagagcatt ttggcctcaa actctttcaa gactccgtag gcgaggatga gctacctgat
    81241 gattttaatt acttcattct cgaaacagga gaaatagaaa tgatcactga gccaaaatat
    81301 agcgtgggtc aaaatctata tctaactttc tattcagaaa atagagaaga tttaacagga
    81361 gattcactag atattatttc attgattcaa aatcgttcga ttcgttttca gagaatggat
    81421 cccaatcatt taaaactaga gaaccaagat cgctatatcg atcaattggt atttacgttt
    81481 agacgattat tgaagagtga ttgtcatggc taaaaatagt tgggagctaa aaataaatgg
    81541 acatgatgaa cttcttgtgc ggatggaacg ctattcaagc gagagcgaac gactgattaa
    81601 cgaagcattg aaatcaaaag gttcggctat tgcagtggat aggattacag aaaaaattcc
    81661 tgtttctgaa gcagatttaa gaagagggca ccaacacgca aaaaatagtc gtccacttaa
    81721 gactcaatac attaatttgg gtttcatcat tagacctaca agaaaatttg agtatttaaa
    81781 atatcctgat ttggggatag gtacttctaa aagaaatcag ccggacgaat ttatgagaag
    81841 aggattaggt cttgcacttg atccaattac agaacttctg attcgtcaat tcgataaatt
    81901 aaataaatag gaggaacaac aatggctaaa acaacaacag taacaacttt tgacaatata
    81961 agtattaaaa gaatttcttt taattttaag aatgcaacaa atgcaatttc aactgattgt
    82021 aatggacaat tagatggcga aacagaaatg cagagaattg taaaaaaatg cggttcaaca
    82081 gaagtaaaat cgaaatctaa accaatcaat atgacggtaa caattactgc acatgtaccg
    82141 atggaagttt atcgacgctt caatgggttg aaacaagatg aacgtattaa atcgggaatt
    82201 tactcttatg gtcctgattc cgtaggggaa gatttctctc ttgctgcaga gatcgtggat
    82261 gatttcgaag aaaaaaataa gctgattggt atgttagcat gcacttcgaa tacaggatta
    82321 acattctcta ttgaaaatgg tgcggatgaa gtagctgcgt tagaactaga aacaaaagtt
    82381 atgcaagatg aatttggtaa attttatcat gaagcaattg ttgcagaact tgaagaagac
    82441 ttaacagatc aatggatgac gaatctatct gctgatgtga ttaaaaaaac ttcagttgtg
    82501 acaactacgg ccactcaatc acagtaaaaa aaacggaggt agcgaaatga acgaagatta
    82561 ctcaaaaatt gaactaaacg atggaacaat tttgaattta gaacctaaac tgaatatcaa
    82621 gaaattattg atgatcaata gagattttaa cacagacgag tttgcaaaaa tgactgtggg
    82681 aaaaggatcc atggatattt ctgttattca aggtgcaaag gctgtgtata ttgcttaccg
    82741 ccaagcgaac atgactgatt atatttcatt cgatgaattt atcgataaat gggattttga
    82801 tatggctact gccagctata tttatcaatt gatgatgttc aaacaagcac gcgatgctta
    82861 tcaaaaagaa tttgaaaaag caaataagga aaaaaagctt caaaagtaaa aatgccaaag
    82921 ctcttagttg aaacgtgggt cgatgtctat tcgatgttga ccgacgtttt ttctatgcct
    82981 tcagatttgg ttttaagcga tatctgttta gatgacattt tgcaaatggc ttacaacaag
    83041 agtgcttatg aaggatggaa aaactatgca ataaaccaat cccagaaaaa ctaaagaaag
    83101 gaggtaaaaa atggctaaaa agagaacaga agcagaagta acattcatag ctaacgatga
    83161 cggattgaaa tctacgttaa aagaaatcag tgctgaatta actaaaaata gagcagaatt
    83221 aaaactagaa caagctcaat tacaacagac tggttctgaa tcagacaagt taggaagtaa
    83281 attatcttct ttagaaaagc agtatgaatt acaaagtcaa aaagttgaag taactagcca
    83341 acgtttagcc aatgccaaaa aatattatgg agaaaattcc accgaagttc agaaacttga
    83401 gagagaactg attaaccaac aaacagcgca acaacgtttg tcaaacgaaa ttgataaaac
    83461 gagtaatgca ctagctcaag caaaaggcga aatacagacg tacgagtcta caatgcaaca
    83521 gttggatagt gaacaaaaaa atgttcaagc tagtgcttct ctgattgaat ccgaatacaa
    83581 aaaatggcaa gcaactgctg gtcaatcagc ttctgaatcc gagaaattag cgaaagccca
    83641 agaatatgtt tctcaacaat ctgaaaatgc agagaaaacg atagatatcc tgagacgaca
    83701 gttagaagct acacagtctg agtttggcgc tacatccaca gaagcaatgc agatggaggc
    83761 gaagcttaat gatgctgaac gtgaatttga agagttagga caagctgcta aaaatgtaga
    83821 tacaactaac ttggacgata tcggaagcaa aatagatatg aataatctaa tggaagcttc
    83881 tgacgtttta agcgacattg gcgataagct tacagaatta gggaaacaag cagtggactc
    83941 tgctaatagt gtaggtagtt cccagagtaa aatacaagct aattttggtt tgactaaaca
    84001 agaggctgaa gaattaacga atgtagccag agacatttat tataaaggtt ttggagaatc
    84061 gttagatcag tccacagatg cattgatttt ggtaaagcgt aatttaggcg atttaaataa
    84121 tcaagattta caaaatatca cggaacaagc tatggtccta gaaaacacca tgggcgctga
    84181 tatggatgaa acgttacgtg gtgtaaatgg cttaatggtc aatttcggct tgagcgctca
    84241 agatgcaatg gatttaatgg tttcgggtac tcaaaacggt ttagataaaa cgcacgaatt
    84301 aggcgacaat atggcagaat atagccaatt atggagtcaa atgggatatt cagctgatga
    84361 aacgttcgga atgcttcaaa atggtttaga tgcgggtgct tataaccttg ataaagtcaa
    84421 tgacttagtt aaggaaatgg gaatatcgtt aacagatggt cgatttgagc aaaacatgga
    84481 tatgtttagt gaaagtacta gaaaagcttt tgaagagtgg aaaaatggcg gaggaacaca
    84541 aaaagacgtt attaattcca tgattcaaga ttttagcaat atggatggtc aatacgacca
    84601 attaaataaa gcttctacaa tttggtctgc acttggcgaa gataacgcga tgaaagttgt
    84661 ccaatctttg actgatgtta accatacatt tgatgatgtt agtggatctg cacaaaaaat
    84721 gaatgaagat tctactactc cgttacaaga attaaacgga aaaatagctg aattaaagga
    84781 ttcattagct cctataggca acacaatcat agatgcactc gaaccagtaa ttgattttct
    84841 aggaaagatg gctgatgcgt ttaataatct tccacaacca gtacaggatt tcattgttgc
    84901 tataggtggt cttacagcag catttggcat actgttaccg gcaatactag ccgtgtcata
    84961 tttatttggt ccgatgatgc ttataattgg aggaattata gctgttatag caggcgttat
    85021 tgtggcaatt aagaactggg gtgcaattac tgactggttt agtggtttat ggaaaaaatt
    85081 tactgattgg ttgggtgata cttgggaaag tataaaagac ggagcctcat cagtttggga
    85141 tggggttaaa gaaacctggt ctggatttgt agattgggtt caagatattt ggcaaggagt
    85201 ttctgattgg tttggagagc tatggagcgg attagttgaa ggagcttcca acatctggca
    85261 aggagtccaa gaaacttggc aaacattcgt tgattgggtt tcaaatattt ggaacggagt
    85321 caaagaagta tggtcgatta tttgggcaga cattgtagga attgttcaaa taccatggac
    85381 attaataacg tcattgattc aagctggtat taatattatc gtgggtattt ttgatgtagc
    85441 tggacagtta ttgggcgcag cttggcaagc tgtttggaca cctatttctg atttccttaa
    85501 aaatacttgg gatactatga cacaatggat aagtatcgct tggaatggga ttgtaactac
    85561 attccatact atatttgatc cagtagtggc atggtggaat ggtatatgga cagctattag
    85621 tactacggct tcaaatattt ggaattcaat tagtgcaaca gcttctagta tttggaacag
    85681 tatcaagaat acaatcacta gcttggtaca agcagctgct acagtaattc aaaatatttg
    85741 gtcaactgta tctagttggt taggtggaat ttggaattca atcagctcta cagcatcaaa
    85801 tatctggaat agcgtgacta gtagtataag caatgctata aacgcagcta aaagtgccat
    85861 tcaaagtgtt tggaatagta tatcttcgtg gattagcgga atttggaacg gtatcaaaaa
    85921 tactgctttg aatctttgga atggaattac aagcactatt agctctaaag taaacgatgg
    85981 aaaaaatgca atttcaagcg gttggtccaa tctaacaggt attgtttccg acatattcaa
    86041 taatgttaaa agtacaattg ctaacatatg ggaaggcatc aaaaagactg ttagcgctcc
    86101 aattgattgg attagagaca aaatcagtgg catttttgat aatttgaata tttctatacc
    86161 acatattccg ttaccggaat ttatcatgga aggcagcttt aacccgctaa aaggtcaaat
    86221 cccccatttg cgtgtaaaat ggcatgctaa aggaggtatc tttactaaac caactttact
    86281 aggtggaatg aacggtgtcg gtgaagcagg acctgaagca gttttacctt tgaaaagatc
    86341 tgttttgcaa gaaattggtg atcgtatctt gagtagcaca tcagtttcat ctagggcaca
    86401 aacgattcaa cctgtgaata actacgaatt caatttcaca attgatggta acgcagatga
    86461 ggttactatg aagcaaacaa ctcaacaaat cattgatagc attacaaaaa ttcaaaatga
    86521 taatgcttcg gcatggcgtt aaacaggaga gtatttctcc tgttttttta gtattaaaaa
    86581 ggatgtgaaa aaatgactga ttgtatacat tctataatcg atggatttcc tgattatttg
    86641 cataaattgg ctttagctga aagaccaacc ataccttctc caaaaagaca gagagttgaa
    86701 acttctgttt taggtaggtt aggtggctta gtacaagatt actcgtttga agatatgtcg
    86761 tttacattgc actataacta tttagaggat gtggaagacc atcaagcgtt caagcaatcg
    86821 ttttatatca tgcgtcattg gttaaactat gcaaagaaat tagaattctc tgacgatccc
    86881 aacgtctatt acgttatcca gactatcgat attggggatg cagaaaacga tattgttgaa
    86941 tggggagagt tcgatgtaaa tattactgcg aaaccattcg caagagttca agaagatgta
    87001 cctataaccg tagataaacc acagtcattt aacttgctga ataatagttt agaagaaagt
    87061 tttccaaaga ttatcatcac tccttcagct acttcatgcc agttcatctt aaatgattat
    87121 gtgtttagtt tcgaaggctt agtagcagga actgatgtag tcattgatag tgatttgatg
    87181 ctttgctacg aagagcaatc ggacggagat attttagatc ggtccaacaa aatgaagacc
    87241 atgcaatatc cgacattgca agtggatatc aatcatttta attgcactgg tttgagcaaa
    87301 atacaaattt atcgtaacgg gttaaggtag gtgaaataga tgatcgataa tttaataact
    87361 atttacgata aaaatgacgc gaataattta gctgaacatt tatatgatac gcaaggttta
    87421 ggcgctttgt cagactggtt aacagctact gttagcaata aactaaacgg agccgagata
    87481 tttcagggta cttattcaat aaacggaact aatgcagatt tgattgtaga cggacgtatt
    87541 attcagtgtt atgtagatga aaatcgagca aaacagcgtc tacggattta ttatgcaaaa
    87601 acttctgtaa taggcaatac gatagaagta aaagctgaac ctattttcaa tgatataaga
    87661 aaatcggtgt tgaataaata tgacagcgga acagaaaaga tcactgctac tcaggcatgg
    87721 caaaacgcaa aagttttagc gaaaccagct atcccttcgc agttttcttt ctcgtcatta
    87781 gtagatacgc ttgctaatgt gaagatagaa aaggcgaatt ttttagaatt ctttggtgga
    87841 aaagagggat ctattctaga tcgatttcat ggtgagtttc taaaagataa taacacatta
    87901 cgtcatgaaa aaagtctagg tacggatcat aaaatcaaag cgatttatac taaaaactta
    87961 actggtcttg acttagagat tgatgctcaa agtgttttag ttggagttta tccattcatt
    88021 agcagctctt cagaaggaga agacgagatc actctaccag aagaagttat tttcacggat
    88081 tacgtggatg attatcctgc tggatatgtt tcttttgttg attttaaaga caaagcgact
    88141 gatgtagcca cattaaggga agctgctaaa gactggttga aaacaaacat agataaacaa
    88201 aaaccacaag tgagtggttc gattgaatta gtaccattga ggcatcaaag aggctatgaa
    88261 aaatttgttg atctagaaaa agtttcgatg ggtgacggag tagatgtgta tcatccacag
    88321 ttaaaagtga atatgtcagc gagaattgtg gaatatacgt ttaatgttct aaccaattca
    88381 tacgataaat tagttgtagg aaacgtcaaa acaaacttct tagaaaacac agagaataat
    88441 gtcagcaatt tgattaatga tgccattgat caattgaaaa acggtggcga aatcagtgat
    88501 ttactcaatg atattgtaga tcatcaaact gatatgatta ctggtcaaaa tggtggttat
    88561 gttttattag atcctaaaga agcgcctagt cgtattttga ttatggacac accagataag
    88621 aataccgcaa ggaatgtttt acaaatcaac aatgctggta ttggtttttc taaaactggc
    88681 attaatggaa catatgaaac ggcatggacg ttagatggcg gattcaatgc ctcgtttatt
    88741 acggctggtg aaatagtagg aattactatt agaggtacta cattaattag tgatggtgct
    88801 gattatagaa caagtattgc taatggcaaa atgacttggt actcaaaaaa agttaacaaa
    88861 gatattatgg agctagaagc acgtgattat gtaagtgctg atgccggtat tgtatcatac
    88921 accatgaaaa ctggtggtgg tttcatgatt agaaatccac agggtaactt ggtttttagt
    88981 acgtgggata atggtaataa cagaccgttt ctatcttttg gtgcgcccaa tttcaggtat
    89041 agcaatgcta gttatgtaac ttctggcgac ggtagttctt taagcattaa tggtagtgcg
    89101 ggtaactcat gggaatttaa ggtagctggt aggactatga aatttactag tgatggtatg
    89161 ctaacgttac caggttgttt ttttggttca tgggaagatg ggaaacttgc taggtttgaa
    89221 caatcaacgg tacaagtata taaagatttt actgttagag gtactaaaaa ctcaactgta
    89281 ccaacagaac attatggaca acgactattg aacgcttatg aaactccaga atattatttc
    89341 gctgattatg gggaagccgt tacaggtgac aatggtaaag ttcgtgttga tattgacccc
    89401 atgtttgctg aaacagtaaa tctaagtcgg tatatgacac atgtgacacc tacagaacta
    89461 gttttgtgtg ctgttactca tgaagatatt gaccatttca tcattgaaac tagtaagcca
    89521 aacgtattag ttagatggaa tttagtggca caccgtctag ggtatgaaga tattagatta
    89581 aaagaggata cagcatatga tagcacagtg cttgaccaaa aacgttttta aaacgaagac
    89641 aaggaggtat ataaatggct agcagtttat ataatttggc tttagatttc agcaaagaat
    89701 taaactacac caaagctatt atggctcgtc aaggtgataa agggattacg gtgacggtta
    89761 aaccgtttct aaatggcttg cagatggata cgagtggcgg aacatttact ttaaaaggaa
    89821 caacaccatc taaccgttac gtagataatg ttgcaactag tgtaactagt gaagaagtca
    89881 cgttttctct tgatggcaca tttatgagtg aagcaggata ttataaacac tgctacgtag
    89941 aatatagaaa agacaatcaa attttaacaa cgcaagatat catttttttc tcactaggag
    90001 tgtctgacat ttcgcaaggc caagccgatg aatatgtttc gcaattagaa gagttgattc
    90061 gaaagtacaa cgaaactttt gatgctttta tggctgaaat caaaggtaga gtggatagct
    90121 taaatcaaca gattactgat ttaactggtc aagctaaaac gctacaagac aagttagatg
    90181 ctctgaaaga agaaatttct aagttaggta acttacaagt gatgtacagt aacagcatcg
    90241 acttcgggga ctatgattat tcggagaacc cgaatttaat gccatatatt acagaacctt
    90301 gggttggacc tttgttaggt aatggacata cagttaaaga tagtgttaaa agggttataa
    90361 ctcatactaa aactagaaca gctaattccg gcgacatatt atcgcttggc ttaggtattc
    90421 cgtgtacagc ggaagctaat aataggtatc ttataaccac tttacgacca tctacgacat
    90481 acacattaag cgttactatg agtgtcggtt cagattggac tggagagacc aatactatag
    90541 gggttaggct tagatattta aatgaacaag gtggtataga actgcccatt aatgcattaa
    90601 taccagcaaa tgtagaacgc gataaaatgg tcactcatac atttactggt ataacaaaag
    90661 ataatgtaac tagcataaca aactgttatg ttgaaatatt ctcacttaat agtgaatata
    90721 aaggaacggt tagtgtcagc tatgacgtta agttaaaagc acactaccct aacttactag
    90781 atggccctta ctggttaggt aaagttcctt taggcgagaa tattgctgac cctacagttg
    90841 tctttccaca caaaactagt gaatatatgg tttatggtag acgcaataca gaaaactata
    90901 tcgctgacca aacatatacc atatcaatga aagcaactaa actaacagtt caatcgtttg
    90961 ccgtgtatat tgctgctggc cgcgttaaag taggagacat gaagcctact gaggggttag
    91021 ctaatacatg ggagttaact tttactgtaa ctaagcaaca tatagatagt ggggtaacta
    91081 attatctgga aatataccaa tacccgtcag ctacaaaagg agctgttcaa attgagtggc
    91141 tcaaactaga aaaaggcaat actcgaaccc cgaatattag tgagtataaa tatcgtggta
    91201 ctggtatgcg tgattcaaac aatccaaaag attatgtttg ggatctagca ccagaatatg
    91261 tcgaagataa tttggccaca gatattaaaa tttctgaaat tactggtaaa gcaaacaatt
    91321 ataccgatgg gaaagtatcg gagattaatt cgcagttgac tgcttcaatt aatgaagtag
    91381 acaccacagc taaggatgct caaacaaaag cgaatgctaa tgcgactgct atagatgaat
    91441 tagacaataa gatcgatgaa cgcattaatg atacagctac taccacatta acagttacaa
    91501 acgggaatac cggatcagca aagctttatc gtgaaggaaa aacagtttct atatattttg
    91561 tggctttaaa cggaaaacgc agtggtggaa atgattcaac gatactaaca attccagaag
    91621 gctatcggcc accaattagt tttgagcaac tggttggctc gatagaccgt tctactttga
    91681 acagtgctca gttatctatt ggtgcagatg gagccattaa atggcgaaga aactcaagtt
    91741 atggatcgga ttataccttt gcaattactt acacgattta gaaaagcgtg aatcgatatg
    91801 aaggcagcat atagaccaat tgaaccttac ggattcgagc aaatcattgt gaatgatgaa
    91861 gaacatttac cggaagaatg cacagaagtc gaaccaccga ttccaaattg gaaaccgaaa
    91921 ttcaattact gggagggaaa taaatgaaaa acatttggaa atatggacgt actggcggag
    91981 agtacgcagg aaaagtattg gacgacatgc ttgtatccgt tccttacaca gatcagccac
    92041 cgcttgaagg aattcgtgct gatggcgaac cgttaacaat cgctgatcaa atgtttgatc
    92101 ctaaattgaa tcaatggatt attttagcga acgcactaga tcacaacgat ttaaacaatc
    92161 tcaaagcaat gtatgagtcg ttagaaaatg agaacggcga tttaaaacag atcaatgcca
    92221 aactcatgct aagcgatgta gcaattaaac aggaaaatac tgcattgaaa gaaaaagcgg
    92281 atagtttagc acaaatcaat tcaaaaatga tgcttgcttc gttacaaaat agcaaagaca
    92341 tttcagaaat taaagagcaa ctaaatccag cttcaaaggg aggtgagtag tatgtttagt
    92401 tttagcgatg tgaaaatgat gtatgattgg ggctgtttca ctaacgaaca agtaatggtt
    92461 ttcgttccgt tgtgcattac tgaagaaaaa gcagataaaa tcattagcaa agaagagagc
    92521 gcatcttaat tgatgtgctt tttattttga ttcaaggagt tgtcacatga ttaatttagg
    92581 ggaatgggga gcgatagcag gatcaataac cgctatcgtt tctttgattt tattagtaat
    92641 aaaaccaatt actgcatctt tctcgaagat tactgagact ctttcaaaag taagtcacaa
    92701 tttagatttg ctgactaaag atttagaatc gagcaaatca gatcgattga tgattcatga
    92761 agaactaaag aaacacgatg aaagattaga tacacatgca gaaaaattgg tagaacacac
    92821 gcaacaaatt aaaactttat ttagggagag aagaaaatga ataataaaac gttcgaagta
    92881 ctaaaatggt tcgcactggt aattattccc gcactagcta ctttcgtggg gttagttggt
    92941 aaagcgctca attggcagta cacagatatc tgtgttgtca tcattactgg ttttggcgcg
    93001 tttttaggga gtgtgttggg tgtatcaaat cgaacctaca aaatgttctc ggctgaaagc
    93061 gaagaaggag gaaacaaatg aaaaagaaaa ttactattac tgcgatgagc ctgttaacgg
    93121 ctcttttttt attacccatt aatacgtttg cttatactat taatgacgag tataatttag
    93181 cgccgaatca aggagactcc agattagcaa ttcctaacaa aattattttg cacgaaactg
    93241 gaatagatgc accagcaaga aacgtagccg ccaacatgaa aaataattat aacggaagca
    93301 attcttatac tacagatgtt attggtgacg gtgggattgt ttaccgtgtg ggtgagcaag
    93361 gatatgtttc gtggggagct ggtaacgcta atccttatgc gcctgtacag attgaattac
    93421 agcgcacata tgataaagca ttgtttgaaa aaaactatcg agcttatatt gaatatacaa
    93481 gagatagtgc aaaaaaatat ggaattccat tgactcttga tcaaggaact tctttattta
    93541 caaaaggaat catttctcat ttgtgggtga caaattatgt ttgggggaac cacacagatc
    93601 catatggtta cttatcgcaa atgggagtta gcaaagaaaa gcttgcttat gatttagctc
    93661 atggatttac cgatgaaaat ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac
    93721 gagctggtgc agctaatcct acgctgacag atggaacaaa tcacgcccac attgatcagt
    93781 ttggggaaat cgaaaacgca aacttgcatg tggctggatg gcacattgct aactataaat
    93841 acgagtatat tttcattatg gactacaata ctgggaaaga attagctcga gtaagagctg
    93901 atggaattta tagaccagat gtaaaccaag cttataatac ttctggaaat gttggttatc
    93961 atgtatcttt caatatgcgt aattttccta gtaagaaagt ctatgtaatg atgcgggcaa
    94021 cgaatgatcc agagggaaac actaaaggcg gtgcgcaaga tttccatgac aaacgttggt
    94081 atttaaatat tcctaaacga taaaaatagc tcctcgttga ggagcagtac ataactatat
    94141 tgacaactat aaaaattatt cgataaaata gtgatgttat cgcatatctt cactatcacc
    94201 cataaatagt cacactccaa gctatgcgat aacaggtttg ttgccacaca ttctactggt
    94261 tgattgttta tggctttatg tggcaacaac cagtaccctt agctcagttg gtcagagcag
    94321 acggctcata accgtccggt cgtaggttcg agtcctacag ggtacattaa cgtagccatt
    94381 tgaatcgttc
//
LOCUS       DAMYXM010000004        93587 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.004, whole genome shotgun sequence.
ACCESSION   DAMYXM010000004 DAMYXM010000000
VERSION     DAMYXM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 93587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.004"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            281..973
                     /locus_tag="PNS21_000334"
     CDS             281..973
                     /locus_tag="PNS21_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDI5158076.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1181..1471
                     /locus_tag="PNS21_000335"
     CDS             1181..1471
                     /locus_tag="PNS21_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158077.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            2185..3555
                     /locus_tag="PNS21_000336"
     CDS             2185..3555
                     /locus_tag="PNS21_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158078.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(3795..3878)
                     /locus_tag="PNS21_000337"
     tRNA            complement(3795..3878)
                     /locus_tag="PNS21_000337"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3842..3844),aa:Leu,seq:gag)
     gene            3933..4112
                     /locus_tag="PNS21_000338"
     CDS             3933..4112
                     /locus_tag="PNS21_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158079.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            4261..5478
                     /locus_tag="PNS21_000339"
     CDS             4261..5478
                     /locus_tag="PNS21_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDI5158080.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            5662..6498
                     /locus_tag="PNS21_000340"
     CDS             5662..6498
                     /locus_tag="PNS21_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI5158081.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            6574..7722
                     /gene="nagA"
                     /locus_tag="PNS21_000341"
     CDS             6574..7722
                     /gene="nagA"
                     /locus_tag="PNS21_000341"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDI5158082.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            7942..9000
                     /locus_tag="PNS21_000342"
     CDS             7942..9000
                     /locus_tag="PNS21_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDI5158083.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            9004..10038
                     /locus_tag="PNS21_000343"
     CDS             9004..10038
                     /locus_tag="PNS21_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HDI5158084.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(10140..10856)
                     /locus_tag="PNS21_000344"
     CDS             complement(10140..10856)
                     /locus_tag="PNS21_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDI5158085.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(10825..11610)
                     /locus_tag="PNS21_000345"
     CDS             complement(10825..11610)
                     /locus_tag="PNS21_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HDI5158086.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(11601..12350)
                     /locus_tag="PNS21_000346"
     CDS             complement(11601..12350)
                     /locus_tag="PNS21_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDI5158087.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            12648..13172
                     /locus_tag="PNS21_000347"
     CDS             12648..13172
                     /locus_tag="PNS21_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDI5158088.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            13188..14687
                     /locus_tag="PNS21_000348"
     CDS             13188..14687
                     /locus_tag="PNS21_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDI5158089.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(14775..15377)
                     /locus_tag="PNS21_000349"
     CDS             complement(14775..15377)
                     /locus_tag="PNS21_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDI5158090.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            15481..16437
                     /locus_tag="PNS21_000350"
     CDS             15481..16437
                     /locus_tag="PNS21_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDI5158091.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(16742..17698)
                     /locus_tag="PNS21_000351"
     CDS             complement(16742..17698)
                     /locus_tag="PNS21_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDI5158092.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            17872..18432
                     /locus_tag="PNS21_000352"
     CDS             17872..18432
                     /locus_tag="PNS21_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158093.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            18425..19249
                     /locus_tag="PNS21_000353"
     CDS             18425..19249
                     /locus_tag="PNS21_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDI5158094.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            19259..19876
                     /locus_tag="PNS21_000354"
     CDS             19259..19876
                     /locus_tag="PNS21_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDI5158095.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            19894..20577
                     /locus_tag="PNS21_000355"
     CDS             19894..20577
                     /locus_tag="PNS21_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDI5158096.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            21260..22495
                     /locus_tag="PNS21_000356"
                     /pseudo
     CDS             21260..22495
                     /locus_tag="PNS21_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            22503..23390
                     /locus_tag="PNS21_000357"
     CDS             22503..23390
                     /locus_tag="PNS21_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDI5158097.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            23407..24243
                     /locus_tag="PNS21_000358"
     CDS             23407..24243
                     /locus_tag="PNS21_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDI5158098.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            24246..24869
                     /locus_tag="PNS21_000359"
     CDS             24246..24869
                     /locus_tag="PNS21_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158099.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            24866..26158
                     /locus_tag="PNS21_000360"
     CDS             24866..26158
                     /locus_tag="PNS21_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDI5158100.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      26517..26614
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            26730..27311
                     /locus_tag="PNS21_000361"
     CDS             26730..27311
                     /locus_tag="PNS21_000361"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDI5158101.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            27314..28621
                     /locus_tag="PNS21_000362"
     CDS             27314..28621
                     /locus_tag="PNS21_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDI5158102.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            28914..29402
                     /gene="purE"
                     /locus_tag="PNS21_000363"
     CDS             28914..29402
                     /gene="purE"
                     /locus_tag="PNS21_000363"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDI5158103.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            29395..30519
                     /gene="purK"
                     /locus_tag="PNS21_000364"
     CDS             29395..30519
                     /gene="purK"
                     /locus_tag="PNS21_000364"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDI5158104.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            30532..31827
                     /gene="purB"
                     /locus_tag="PNS21_000365"
     CDS             30532..31827
                     /gene="purB"
                     /locus_tag="PNS21_000365"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDI5158105.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(32007..32384)
                     /locus_tag="PNS21_000366"
     CDS             complement(32007..32384)
                     /locus_tag="PNS21_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158106.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            32640..33368
                     /locus_tag="PNS21_000367"
     CDS             32640..33368
                     /locus_tag="PNS21_000367"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDI5158107.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            33368..33622
                     /gene="purS"
                     /locus_tag="PNS21_000368"
     CDS             33368..33622
                     /gene="purS"
                     /locus_tag="PNS21_000368"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDI5158108.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            33624..34295
                     /gene="purQ"
                     /locus_tag="PNS21_000369"
     CDS             33624..34295
                     /gene="purQ"
                     /locus_tag="PNS21_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HDI5158109.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            34296..36518
                     /gene="purL"
                     /locus_tag="PNS21_000370"
     CDS             34296..36518
                     /gene="purL"
                     /locus_tag="PNS21_000370"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDI5158110.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            36503..37942
                     /locus_tag="PNS21_000371"
     CDS             36503..37942
                     /locus_tag="PNS21_000371"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDI5158111.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            37965..39017
                     /locus_tag="PNS21_000372"
     CDS             37965..39017
                     /locus_tag="PNS21_000372"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDI5158112.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            39014..39592
                     /gene="purN"
                     /locus_tag="PNS21_000373"
     CDS             39014..39592
                     /gene="purN"
                     /locus_tag="PNS21_000373"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDI5158113.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            39589..41124
                     /gene="purH"
                     /locus_tag="PNS21_000374"
     CDS             39589..41124
                     /gene="purH"
                     /locus_tag="PNS21_000374"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDI5158114.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            41140..42414
                     /gene="purD"
                     /locus_tag="PNS21_000375"
     CDS             41140..42414
                     /gene="purD"
                     /locus_tag="PNS21_000375"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDI5158115.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            42536..43450
                     /locus_tag="PNS21_000376"
     CDS             42536..43450
                     /locus_tag="PNS21_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDI5158116.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(43583..45148)
                     /locus_tag="PNS21_000377"
     CDS             complement(43583..45148)
                     /locus_tag="PNS21_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDI5158117.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(45141..46592)
                     /locus_tag="PNS21_000378"
     CDS             complement(45141..46592)
                     /locus_tag="PNS21_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDI5158118.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(46606..47622)
                     /locus_tag="PNS21_000379"
     CDS             complement(46606..47622)
                     /locus_tag="PNS21_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI5158119.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            47842..48930
                     /locus_tag="PNS21_000380"
     CDS             47842..48930
                     /locus_tag="PNS21_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HDI5158120.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            48984..50510
                     /locus_tag="PNS21_000381"
     CDS             48984..50510
                     /locus_tag="PNS21_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDI5158121.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            50512..51717
                     /locus_tag="PNS21_000382"
     CDS             50512..51717
                     /locus_tag="PNS21_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDI5158122.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            51974..52951
                     /gene="guaC"
                     /locus_tag="PNS21_000383"
     CDS             51974..52951
                     /gene="guaC"
                     /locus_tag="PNS21_000383"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDI5158123.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            53215..54639
                     /locus_tag="PNS21_000384"
     CDS             53215..54639
                     /locus_tag="PNS21_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDI5158124.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            55100..56209
                     /gene="pdhA"
                     /locus_tag="PNS21_000385"
     CDS             55100..56209
                     /gene="pdhA"
                     /locus_tag="PNS21_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDI5158125.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            56212..57189
                     /locus_tag="PNS21_000386"
     CDS             56212..57189
                     /locus_tag="PNS21_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDI5158126.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            57214..58857
                     /locus_tag="PNS21_000387"
     CDS             57214..58857
                     /locus_tag="PNS21_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDI5158127.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSASSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            58865..60271
                     /gene="lpdA"
                     /locus_tag="PNS21_000388"
     CDS             58865..60271
                     /gene="lpdA"
                     /locus_tag="PNS21_000388"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDI5158128.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            60433..60708
                     /locus_tag="PNS21_000389"
     CDS             60433..60708
                     /locus_tag="PNS21_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDI5158129.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            60714..61481
                     /locus_tag="PNS21_000390"
     CDS             60714..61481
                     /locus_tag="PNS21_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HDI5158130.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            61649..63478
                     /gene="typA"
                     /locus_tag="PNS21_000391"
     CDS             61649..63478
                     /gene="typA"
                     /locus_tag="PNS21_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDI5158131.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            63739..65511
                     /gene="recQ"
                     /locus_tag="PNS21_000392"
     CDS             63739..65511
                     /gene="recQ"
                     /locus_tag="PNS21_000392"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDI5158132.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            65625..65927
                     /locus_tag="PNS21_000393"
     CDS             65625..65927
                     /locus_tag="PNS21_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDI5158133.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            65930..67093
                     /locus_tag="PNS21_000394"
     CDS             65930..67093
                     /locus_tag="PNS21_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDI5158134.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            67119..70547
                     /locus_tag="PNS21_000395"
     CDS             67119..70547
                     /locus_tag="PNS21_000395"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDI5158135.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            70568..71692
                     /locus_tag="PNS21_000396"
     CDS             70568..71692
                     /locus_tag="PNS21_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158136.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            71682..72119
                     /locus_tag="PNS21_000397"
     CDS             71682..72119
                     /locus_tag="PNS21_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDI5158137.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            72103..72441
                     /locus_tag="PNS21_000398"
     CDS             72103..72441
                     /locus_tag="PNS21_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDI5158138.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            72560..73120
                     /gene="rsmD"
                     /locus_tag="PNS21_000399"
     CDS             72560..73120
                     /gene="rsmD"
                     /locus_tag="PNS21_000399"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDI5158139.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            73117..73608
                     /gene="coaD"
                     /locus_tag="PNS21_000400"
     CDS             73117..73608
                     /gene="coaD"
                     /locus_tag="PNS21_000400"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDI5158140.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            73598..74641
                     /locus_tag="PNS21_000401"
     CDS             73598..74641
                     /locus_tag="PNS21_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDI5158141.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            74800..75528
                     /locus_tag="PNS21_000402"
     CDS             74800..75528
                     /locus_tag="PNS21_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDI5158142.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            75644..75889
                     /locus_tag="PNS21_000403"
     CDS             75644..75889
                     /locus_tag="PNS21_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDI5158143.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            75886..76104
                     /locus_tag="PNS21_000404"
     CDS             75886..76104
                     /locus_tag="PNS21_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDI5158144.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            76240..76779
                     /locus_tag="PNS21_000405"
     CDS             76240..76779
                     /locus_tag="PNS21_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDI5158145.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            76772..79045
                     /locus_tag="PNS21_000406"
     CDS             76772..79045
                     /locus_tag="PNS21_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDI5158146.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            79083..80129
                     /gene="holA"
                     /locus_tag="PNS21_000407"
     CDS             79083..80129
                     /gene="holA"
                     /locus_tag="PNS21_000407"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDI5158147.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(80218..80472)
                     /locus_tag="PNS21_000408"
     CDS             complement(80218..80472)
                     /locus_tag="PNS21_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDI5158148.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(80872..81141)
                     /locus_tag="PNS21_000409"
     CDS             complement(80872..81141)
                     /locus_tag="PNS21_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158149.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(81611..82066)
                     /locus_tag="PNS21_000410"
     CDS             complement(81611..82066)
                     /locus_tag="PNS21_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDI5158150.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(82204..84132)
                     /locus_tag="PNS21_000411"
     CDS             complement(82204..84132)
                     /locus_tag="PNS21_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDI5158151.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(84329..85162)
                     /locus_tag="PNS21_000412"
     CDS             complement(84329..85162)
                     /locus_tag="PNS21_000412"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDI5158152.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(85354..86058)
                     /locus_tag="PNS21_000413"
     CDS             complement(85354..86058)
                     /locus_tag="PNS21_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDI5158153.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(86081..86707)
                     /locus_tag="PNS21_000414"
     CDS             complement(86081..86707)
                     /locus_tag="PNS21_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDI5158154.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            87033..89408
                     /locus_tag="PNS21_000415"
     CDS             87033..89408
                     /locus_tag="PNS21_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDI5158155.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(89432..89923)
                     /locus_tag="PNS21_000416"
     CDS             complement(89432..89923)
                     /locus_tag="PNS21_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDI5158156.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            90110..91030
                     /locus_tag="PNS21_000417"
     CDS             90110..91030
                     /locus_tag="PNS21_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDI5158157.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            91184..91528
                     /locus_tag="PNS21_000418"
     CDS             91184..91528
                     /locus_tag="PNS21_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDI5158158.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(91718..92218)
                     /locus_tag="PNS21_000419"
     CDS             complement(91718..92218)
                     /locus_tag="PNS21_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5158159.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            92746..92829
                     /locus_tag="PNS21_000420"
     tRNA            92746..92829
                     /locus_tag="PNS21_000420"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:92780..92782,aa:Leu,seq:cag)
     gene            complement(93153..>93587)
                     /locus_tag="PNS21_000421"
     CDS             complement(93153..>93587)
                     /locus_tag="PNS21_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDI5158160.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        30492 a        16046 c        19930 g        27119 t
ORIGIN      
        1 cgttttttgt aataaataca gtaatctatg aaatgaaaag ggttacgttt aataaaactt
       61 gcttttttgt ttttgttcac cataaatatt tatttaagaa agtattaacc tgcatttttc
      121 tctataaaaa agatttttta aatagataaa aaaggaggat taaatgagta tttgatagta
      181 tttatattaa aattacacat gtaatttaaa aacgcttgac attgtgtttg agatcactta
      241 cttttagcat gtaaaagtat tggattaagg agctatgcaa atgaaaaaga aatttattac
      301 tggtgggatt gtcgttgcat ttctatgtct aattggctac acaggcacgc ggtacgcaaa
      361 tgaaattatt ttgtggggtg gagagacaga tattgataca atcaatgaga atctgacaca
      421 gcttgatcat acattgacag ataaagagca agcgcttaca gatcttaccg ctcgactagc
      481 acaaaaagaa cagacattgc aagattcaaa ggcagatgtc caagaatacc aaacaaaagt
      541 aaatgaatta gaaaaccaaa aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca
      601 attagtcagc aaggatgcac aactacaaag caaacaaaat gaaatcaatg tcaaactaga
      661 tgagatcaac caaaaaaatg atgaaatcaa tcatctgaat gcagattgga cagcaagact
      721 acaagaagca caagcaaaac tttcagaatt acaaaatact gtaaatggtt tgaatcaaca
      781 aatcgcaaca ctgacacaag aaaaaacaaa tgtagaaact cagttaacag atacacaaaa
      841 gaaattagac gaagcaactg ctgaaaactc aagcttgaaa caatatattc aaaagttaga
      901 aaaagcaaaa gatgaagtgg aagatacagc tgataaatct cagcaaattg ttgaagaaca
      961 tttgcgtaaa taatctttct taacaagtaa acctaaatta gtaaagaagc aacagtaatc
     1021 tatactgctg cctctttttt ttagtacatg tccttcatct tcgcaggaag tgccaatatg
     1081 attttaagaa aggtaataga caaaaagtca aaaagataaa acagagtttc ttctttacaa
     1141 agggaatgta tgttcgtata atggtattga ggtgaaggtt atggagaaaa aatatggaac
     1201 agtagataag atccgtactt tgcaagttag tgccagaccg ttgatccgtt tcagtttaaa
     1261 tggggtcaat tgtttgattg cttcccatag tttgaacttc ctagctgaag tagacgaagg
     1321 gatgaagtta gtcgttacag gttattataa taatagaaag caatttgttg tgagatccta
     1381 tcatgtgtta gggaaaccca aaattgtggt agaatatgag aaaagtctat acccaagaaa
     1441 aaaggtccga caagatgaaa gaataaacta gaaaacaagc ggctgagaca aaaatgtcct
     1501 agccgcttgt tttggaaaga tggatgcaga cgatgggatt tgaacccaca cgagcatacg
     1561 ctcacaagaa cctcaatcta tatataacta catgtattta agtatttgtg atgaatcact
     1621 aaactttcca aactcatttg agggatattg cttaaagatt gaacagatca attcgaagcc
     1681 ctattagtat tcgctcaatg gggggatcag aggccataaa aagagactag tcattactag
     1741 tctcaggtag tggaattgta ggattttaaa cgttcctgga gagtaccggt atgtaactcg
     1801 aagaggtgat tatcatagtc gtaaaagtaa attgaagaag cctcgccttt cacacgagaa
     1861 cgtcctggtt cgacagttaa acctaacatt tggatttttg agacaaacga atctatatct
     1921 tgttcgtcaa ttttaaaagc aatatgattg tatgtctttg gcagtttatt cgacgaattc
     1981 tgcattactg caatccatag gtccttaact aagaaaaatt tttctggata taatgaaaat
     2041 ttttttgttg actatcgtag atttcttttg catcaaataa ggtatttaac aattaagtat
     2101 ttaaaagata acttttaagt tatctttaag taatgcttaa agctatgtta tactctatta
     2161 ggaaatataa gaaggagtat aaaaatgaac aaacataata gaaaaataac gaataacaaa
     2221 gaactgatct tgttactaat agggatttgg ggtatctttt tactctttaa tatgtatatg
     2281 ccgactatga gagcggatga cgtagtttat gcaagtagat tagatgaact tggctatttg
     2341 ggtgcttcga tagaacatta taagacatgg agttcaagaa ttattataga actattctta
     2401 atgttctttt ctaaacactt tatgctatgg aaattattaa attctacgat tatgttagga
     2461 attgtagtat tactttgcaa gtatgttttt gagaaaataa atgttaaaaa tttattattg
     2521 gtttgctcta tatactgttt aataccattg acagtaatgg gggaaaccgg ttggagagca
     2581 accaccttga actatcagtg gccagtagca tttagtttac taacttttta tccgtttttt
     2641 caactcttaa gaggagaaga aataaataga aaaatatatt gggtaagtat tcctttattg
     2701 atatttttaa ctaatcaaga acaagtcaac gcctgttttt ttgttctaac aagtatagtt
     2761 agtttatatt tgattgtcaa tggacgatat aattataagt tatcagtatt ttcaataata
     2821 agcttagcag aattaatttt ctctttgaca actccaggga atgcattacg tgctgcacat
     2881 gaaattaata aatggtttcc tgaatataaa aatttcaact ttcttaataa gttagattta
     2941 ggtatttcat catttggaaa gccgtttttt ttagacatga atatcttatt tttattgcta
     3001 tttttcttaa ttttccttct aacatatagg aaatgtcaaa actattatgt gcgtatattg
     3061 actgcattac cgtttttctt aaacctgatt atttattttg gtaatacaat gggtcaaagc
     3121 tttacttacg tcaatggaaa taaaagggca atgatttgga gtagtagtaa cctgaataat
     3181 ctctttacag agttaggaac gaaactgtca cttttttatc caggtacttg gatagctact
     3241 ttagtagtct tggcattatt actatgttta attgttggaa tttatttaag ttttgacaat
     3301 aagaaaactt ctattttttt agttattttg atgataatgg gattttgttc aaggttaatt
     3361 atgggatttt ctcccacagt atgggcttcc ggaatgcgga cgtactatat actttatgtt
     3421 gttatcgcca tcttagtact aatggcagtt aaagagctaa tgaaaagcat gagtgtacaa
     3481 aaaaatgaat ttatgcaatt tggattaacc gtgcttggta tttgtacctt tataataacg
     3541 gtgataaaca gataatatac aaaaaatccc tcgctttagt attaggtgag ggattttact
     3601 cgtttttcga ttaattgttt taacacttgc aagtttctac gagtagcttg tttccgacct
     3661 tcatatactc acctcaggct gtttacatga ttgtacgttc tttttgtaaa ggatacagtg
     3721 atatgtatat aatggcataa agaaacgttc aaacaaaaaa actcgtgaag caaaattaac
     3781 ttcacgagtt gtagtgcaga tgatgggact cgaacccaca cgagctaacg ctcacatgac
     3841 cctcaatcat gcttgtctgc caattccaac acatctgcgt aataataaca cactaatgat
     3901 agcgaggttt tacatagttg tcaagctcaa aaatgggagg acttattaag atggagaaat
     3961 atgacggaga attttcagga ctgggaatga tcttaggtgt attgattgga cttgcattcg
     4021 gacgtttttt gcttggtttt atgctaggaa tcatttgtgg aattgcaatg gattgggcag
     4081 cgaatctatg gaatgactat catgacaatt aagttctcaa cgcttgtttg tgttttttta
     4141 atacttacgt ttttcctaaa taagtggcaa aattttctag gatggagtat gatattgaaa
     4201 tagtaaataa caaaaaattt ggaaagttct aactttttga aatgagggat tggctaagtg
     4261 atgagagaat ttgaaaaatc caacaaatta gaaggtgtca gttacgatgt tcgcggtccc
     4321 gttctagaag aagcggagcg gatgcaagaa gagggcatta gtattttaaa attgaacacg
     4381 ggaaatcccg caccttttgg atttgaagca ccaaacgaag tcattcgtga tttgatcatg
     4441 aatgcccgtg actcagaagg ctactcagat tccaagggga tcttttctgc tagaaaagca
     4501 attgagcaat attgccaact gaaacatttt ccgaatgtga cgatcaatga tatctacacc
     4561 ggcaacggag tgagcgaatt gatcacgatg tgtatgcagg ggttgctgga caatggagat
     4621 gaagtactgg ttccaatgcc ggactatccg ttgtggacgg cttctattgc attagctgga
     4681 ggcacacctg ttcattatat ttgcgatgaa gaggcagaat ggtatcctga tattgatgat
     4741 atcaaatcaa aaataacgag tcggacaaaa gctattgtta tcattaatcc taataatcct
     4801 actggggcac tttatccaaa agaattatta gaagaaatcg ttgaagtagc tcgacaaaat
     4861 aacctgatta tttattccga cgaaatctat gatcgcttag tgatggatgg tctagaacat
     4921 attccgattg ctacattagc gcctgatttg ttcgttgtta cgctgaacgg tctatccaaa
     4981 tctcatcgtg tagcaggatt tcgagtaggc tggatggtcc taagtggaga taagtcacac
     5041 gtcaaaggct atatagaagg gctgaatatg ttgtcctcca tgcgtttgtg ttccaatgta
     5101 ttgtctcagc aaattatcca aaccgcgcta ggcggttatc aaagtgtcga tgagctgctc
     5161 ctgccaggcg gaaggatcta tgaacaaaga gaatatatct ataatgcaat caatgatatt
     5221 cctggtcttt cagccgtaaa accaaaagct gctttttata tattcccaaa aattgataca
     5281 aagcgattca atattttgga tgatgaaaaa ttcgttttgg actttttaca tgaacatcat
     5341 attctacttg tacatggtgg tggatttaat tggaaccagc cagatcattt tcgaatcgtt
     5401 tatcttccta aaatggatga tttgaaacaa acagctaaga aaatgagaga attcctagca
     5461 acctatcgcc aaaaatgatt ttaaaaacca aatttttcag taggagggcc acagacaaac
     5521 acactgaggc tctctttcta gtggagtttt cttaaataac taaagtatat tatttagttg
     5581 caagtctttt cttgaagttt gcgacttcct aaagtaaaat acgaatagaa tgcgaatacg
     5641 catattttgg ggaggatact tatggaaacg ttaatcgagt cctttggaac gatcattaaa
     5701 gagattcgaa aagaacaaaa aatgacgcaa aaaatgcttt cgcatgatat ttgttcacaa
     5761 agtgtattaa gtcggatcga aaataatgag gaactgccga atgtattggt gatgatgcag
     5821 atttgtcaac ggctaggggt aacgattgat catgtgatga atctctctcg aaaaactact
     5881 cggacaaacg ataaattatt tgaactgttg gatagttgtt tccaaaaacg tgattatcgg
     5941 aagttagaac aagtactaaa atcttctgat atttctgaaa atctgtatca agcaactgac
     6001 tttcaacgct attattatta tctcggtgtt tgcgaattca cgttgcgtca aaatatctct
     6061 cagtcccttt attatttgaa agaagcatta agctactcta gccaaaaaga aagaatccat
     6121 gtttctgaca cagaaataca actgattagt tgtatcggaa aaatatatgg tgtggcagga
     6181 aaaacggctg aagcacgcta ctatttgagt cgaagcatcc aactgctgca tcaatcagct
     6241 gatgaacgat caaaaagtga attttcgcaa attttttata attatggaaa tttcttattc
     6301 catcaaaatg aaatcgacga agcgttagaa caggtgaatc aaggaatcta ttgggctcaa
     6361 gagaagaata gctactatta cctcaatgat ttatttgtgc ttaaaagctt aatatacaag
     6421 cgtaaagaag aaccagaaag agcagcattc tatgaaggct tagcacaagc tgtgaaacaa
     6481 atttcaaaaa atttgtaaat ggtgtagacc attcttgact tcttctctaa ataagagtac
     6541 tttagaggta gaaaaacaaa gggagagaat agaatgagaa cttttatcta tgctgacaaa
     6601 ttctttttag cgtcaggtgt caaacatgca ggctatcttg aaattaccga tggacttttt
     6661 ggtgaataca ccaaagaact acctgaggga gaagtcaaca tcattgacca aagcggcaaa
     6721 tggattgctc ctggtttggt agatacacat attcacggct atatgaatca tgatgtaatg
     6781 gataatgatg cagaaggcat caaagtgatg tctgaaggtc tgttatcttg tggagtgact
     6841 tcatttttac caacaacatt gacgtcaagc aaagaacgtt taaaagatgt agctgagaca
     6901 atcggcaaaa tgtatcagga agcaccagga gctaaaatca aaggaatcta ttttgaaggt
     6961 cctttcttca cagaagaaca caaaggggca caaaacccaa gttatttcgg ggatcctgat
     7021 ttggatactt tccatgaatg gcaagaagct tcaggtggtt taatcaaaaa aattgcttta
     7081 gcaccggaac gtcaaggtgt agaagagttt gtcaaacaag tgactgaaga aggagtagtt
     7141 gtttctctag gacacagtaa tgctacacta gaagaagcgc aaaaagctgt tgaagcaggc
     7201 gcaagtgtat ttgtccatgc tttcaatggt atgcgtggat tgaatcatcg cgaaccaggt
     7261 atggtgggtg cgttattatc tttagaacat gtcttttctg aattgatttg tgatggccat
     7321 catgtacatc caaatgctgc gggaatccta atggaaaaag caggacatga tcatgtcgca
     7381 ttaattacag actgtatgat ggccggtggg atgcctgatg gaaattacaa tttaggcgaa
     7441 tttccagtag tagtaaaaga aggaacagca cgtctagaat ctggcaactt agctggaagt
     7501 atattgaaat tgaaagaagc aattaaaaat gttgtagact ggggattagc aacaccagaa
     7561 caagcagtca tgatggctag tctgattcca gcaatcagct gtaaaatcga tgatacatgt
     7621 ggaaaaatcg ctcaaggacg cgatgcagac tttatcgtat tgaaccctga tatgacattg
     7681 gaagcaactt atctagatgg cgtggaacgt ttccacgcat aatataattt aagttaacct
     7741 taaaactaaa cttcttaagt ttggttttaa ggtttttttg tgttctagta aaaatcactt
     7801 tacataagtt tcaaaagttt ctttaatttc ctattgttca ataagtaaca atgtaaagat
     7861 aaaattaaaa atgttaatac gtattagcaa aataaaagct ttttttattg tgaataaatg
     7921 aacataatta ggaggagaaa gatgaactta tttgacattt ttcgatcgac tttgacagat
     7981 atgtcgattg tcagtgcaat cacttcaaca gtttttatta tattattagg gtttgtttgt
     8041 agaaaaaagg ggattttttc tgaatctgtt ggaaaaattc tatcgaaagt cgtcttaaca
     8101 gtggctttgc cagcactggc atttcgagct ttcatgcaag atatcaatac agaattactg
     8161 atgcaaggaa tcaatgtctt gatttgggga ttagttattt atattattct tattctagtt
     8221 tcaaaacctt ttttcttaag ctataaaggg gataagcaaa caacgttgcg tgtcctgacg
     8281 atctttggtt ctacgacctt tttcggaacg cctatcgtca gtgcgattta tggtcctgtc
     8341 ggcgtgatgt attcctcaat tttcaatatt ggctaccgaa tctttttgta ttcttatgga
     8401 tacataaaaa tgagtggctt gaagatggaa gctaaaaata tcaaagcgat gttcgtcaat
     8461 ccaatcatta tcgctacttt tgccggcctt ttcatttggc tgttccaagg gtatctgcca
     8521 caagtgtcag ttacaggtgc agaaggagag ataagccaag tagcgttttt gcgaattgac
     8581 cagactgcac cgtggctatt tcagccgatg gattatttag ctggtttaag ttcaccattg
     8641 gcgtggctag caatcggcgc aacattaggt gaagtaagct tcaaaaaagc tgcatctgaa
     8701 aaaacttcat ggtattacag tctagtcaaa gtggtatttg tacctgcatt gaatatcgtg
     8761 attctagcag ttttgacggc aacgaatatt ctaccagtca attttgaagc actggcaaca
     8821 atcgttatca tgatggctac accagctgca acagtggcag cagcctatgc tatcagtttt
     8881 gatagggagg cacttttagc ctccaatgca tccttactgt caactatttt tgctgtggta
     8941 ctgacacctg tatggatcgt tgtattacaa atgattcatg gcataggttt attttcataa
     9001 gaaatggagg aagaaagtat gagaatcgca tgttacggtg ttcgtccgaa cgagatagat
     9061 ttttttgaaa aattgaatat ttaccattat gatctcagtc tttatgaaga actgttgaca
     9121 catgacaata tcgtgtcagc aaaggaccat gatgctgtat tgttgagggg aaattgtata
     9181 gcagatgaga ccaatcttgc aaaaatgcag gaatatggga ttcgttatgt ttttacacgg
     9241 actgtgggag tcaatcatat cgatctacaa gcggcagctg attttggtat gacagtagcc
     9301 cgtgtaccat cgtattcgcc aaatgcgata gcagagttat ctttgacttt tgccatgatg
     9361 cttttgagaa atactgcgta tacgacgatc cgaacatctt tcaaagattt tcgagtggat
     9421 gagcaaatgt ttagtcgtga aatcagaaat tgcacagtag ggatcatagg aacaggcagg
     9481 atagggttga cggaagctgt tttgtttaag ggactaggag ccaaagtgat cggctacgat
     9541 attcatcaga cagaagctgc taaaaaagta ttgacgttcc actcgcttga agagttatta
     9601 gccaaaagcg atattgtcag tattcatgta cctcatatcc caggtgaaaa cgatcaaatg
     9661 atcaatgaag gatttttagc tcaaatgaaa aaaggtagca tcttgattaa tacagcccgt
     9721 ggagagcttc aagataatga agctatttta agagctttga aaagcaatca tttagctggt
     9781 tttgcaaccg atgtttttgc aaatgaaaca gaggtttttt tccgatcctt caagccatgg
     9841 gagacgattc cagaccctgc tatccagcaa ttagtagaaa tgtaccctag agttcttgta
     9901 accccgcatg taggttcaaa tacagatgag gcattatcaa atatgatttc gacaagtttc
     9961 gagaactttc gtgaaataat agaaactgga aaaacgaaaa atgaggtatc tcttcctaaa
    10021 gcccgtcaat taagataaga aaacatggat attcgtcaat aaaatgttta aaaatagtcc
    10081 aagtaaacag ttgttacttc tgtatacttg gactattttg tatgggataa ttcgatgact
    10141 caacagtaat ctgtgagctt tttccgccca ttctttgtca attcataaca ggttagcggc
    10201 tggccgattt ccaaatattt gattctttcg ttcaatatat cttgttctac taaaaaattc
    10261 aaatatttct tgacggatat acgtgacagt gagctttttt gagccagttc ttttgttgaa
    10321 aaaatagtat cttcttttag aataaatgca cttatttttt ttaaagtcag ttttgacaga
    10381 cctttaggca aatcgatgaa tgatggtatt tcgtgaatgg gagtggtgtg gttgaatact
    10441 tgatcgatca atttctgatc ggctttttca gtcgtttgga ttgtctgaga gaaagcaaga
    10501 tacttttcga tcgccagctg aaaacgttgc aaagtgaacg gtttgatcaa ataatctaag
    10561 gcaccgtagc ggatcacatt ctttatggtt tcgatatcat ctgcagcagt gatcaaaatc
    10621 actgggatat catttccttg ctgtctaagc caagataaaa aatggatacc actttcgcca
    10681 ggcatgtata catcaagcag cagcagatct gcgttttttt gtctgaaata tttttttcct
    10741 tcttctacat ttctgaaact ttcgattacg ttcatattag ggatgctttc cacaaaagta
    10801 cgattcaatt ctgcaaccat tggatcatct tcgattatca ggacatttgt catcattttg
    10861 atcttccttt tttggtaatt ctagatagac gattgtaccg ccatttgttt gtttggaaaa
    10921 ttctaaaaat ccaccgtggc gtttgacgat tgaatggatg agattcaatc caaacccacg
    10981 attgattccc ttcgtcgaaa aacctagcgt aaatattttt tctgagattt cttctgatat
    11041 cccgacaccg ccatcgcaga cggtcacgat gaatacttca gcttctgtgt catagtttaa
    11101 atgaatccgg acctgtccgt ttctttcatg tttttgtagc acggcttctt ttgcattatc
    11161 aagtaagttc cctaacactt gcaaaagtga atggatggag tcatccatct ccattttagg
    11221 gataaaagaa tcgctttcta acagcatttc aacagattgt tctttggctt cgtttatttt
    11281 gcctaataaa aaaccggcaa ttgcaggtac tttgatcaga cttgagactt ggccaatctc
    11341 ttttttataa ttttgattta gctgctggat aaaatgggtg acttcttcat acttcttcaa
    11401 atcaatcaaa ccagaaatga catgaagttt attcatgaat tcatgggttt gagctctcat
    11461 ggagtttata tattgtttcg tccccccaag ctcctccatt aatttcatta cttctgatcg
    11521 atttcgtaaa gttgcgactg ctcctaaaaa acggtcattg ctaaaaagag gtgtaacgct
    11581 agaaatagtt tctatcccat ttaaatacat ggattgagtc cgaacggtcg tttgatgctc
    11641 aatcgtctct ttaaacaaaa cgtcatatag attatcactg atttttttcc tacttcgaaa
    11701 tccaatagct gctctgtttg gtagaaaatg ttttttgcag gtgtattgat caagaggata
    11761 gttttatcag aagagatggc taagattcct tcatctactt cattttcgat aatcgttttt
    11821 tcacttaact gttctgctat ttcttgtggt tcaagtccaa gcagtacttt tttcattttt
    11881 tgggcaagaa aaacggcgcc tatgatgcct acgctaagtc caataatcaa tgaaaaaaca
    11941 atcatttttt gtccatcttt gacttgattg tcaatcgtgt tgtttgttaa gccaacacaa
    12001 actgcaccaa taatctcttg atcataataa atagggacga aatagcggaa gccgaaccct
    12061 aacattcctt ctttttcaga gaaaaattct tgtcctccaa tcactttttt cacatcttca
    12121 tgacttgaga aaggaagacc gatgtaactt gggtctgcgt gagaatatcg aatgccttca
    12181 gggtccatga caacgataaa atctaaattc gatgcaatca tgatttcatt tgttattcct
    12241 tgaatcgcat gtgtcgggtc accagtctgc agcttttttt ggatgagttt atcattggca
    12301 acgattttag caatgctaga gattttatct gacacactag atcggttcat cgaaagaata
    12361 aaattcctga tcaatatggc agaaacaatc aaagaaacga atgtggagaa acaaacgaca
    12421 aggatgatcg tgttctggag ctttaatggt tttttcttaa aaaaaggaaa cataggctac
    12481 ctcgaaagaa tttattgtga aaattttaac atgaaaacaa aaatttaaca aattgtcaac
    12541 gataaacaca accttcctat aaaaaacacc actgagtcgg taaaagaagc tatacagaat
    12601 tttcaaacat tggtatgata gacgtagaaa tggaaggagg cgtgtatatg atagggggaa
    12661 tcgcacttga gacgatttat tggtatacat tgctgatttg tgcaggattg gcagtcctat
    12721 tgattatttt tggagatgta ttcgattttg atggcccgct tgatccgatg cttattatcc
    12781 cttggttaac tttcacaagc ttatttggtt ttctaggtga gcgtttaaca gatcaatcat
    12841 ctttattttt atttattgct agtggaggca tatcgatagt gttggtattt ttgcttaatt
    12901 tctatatatt gatgccgatg aaacatgccg aatcaacgat gtcgtcttct gaaaaaactc
    12961 tggaaggaca agtagctgtt gtgatcacac cgatccctgt acgaggtatg ggagaaattc
    13021 agttgaaaag tgttaccggt tccattagtc gtccagcttg tttttacagc ccccaagaac
    13081 agacagctga gcggggaacg aaagtattga tcattgagat caaggaaaga atctgttatg
    13141 tcatgcctta tgaaagcatg ataaaagctt aaaggagcgt gtgttaaatg gaaataccag
    13201 ttgtttttat cgcgatcgca atcgcagctt tgattttact gatgttgctg atcgtctttg
    13261 tagcgaagta ccagacagca aaaccggatg aagcgttgat catcagtgga agttatttag
    13321 gaagcaaaaa tgtgcacatt gatgaaagca ataacaaaat caaaattgtg cgtggaggtg
    13381 gcgcattcgt cttgccagtt tttcaacgtt cgaatcggat cagtctatta tcgagtaaat
    13441 tggacgtctc cacccctgaa gtgtatacgg aacaaggcgt accggtgatg tgtgatggta
    13501 cttcgatcat caaaatcggt tcatctgtag aagagattgc tacagcagca gaacagtttt
    13561 taggcaagac aagagaagaa ctggaaaatg aagcaagaga agtattagaa ggacacttgc
    13621 gctcgattct tggttctatg actgtagaag aaatctatca aaatcgcgat aagttcagtc
    13681 aaagcgtaca agaagtcgct tctgtagacc ttgcaaaaat ggggttgatc atcgtttctt
    13741 tcactatcaa agaggtcaga gacaaaaacg gttatcttga ctctttaggg aaaccaagaa
    13801 tcgcccaagt caaaagagat gcagatatag cagaagcgga agctctaaaa gaaacgcgta
    13861 tcaaaaaagc tcaagcagaa aaagaatcac aagcagctga attacagcga caaacagaga
    13921 tcgcagaatc attaaaagaa aaagaactga aattagcaac ctacaaacaa gaacaagatg
    13981 ttgctaaagc aaaagcagac caagcctaca atttagaaag tgcccgagca caacaacaag
    14041 taatcgaaca ggaaatgcag attaaaatcg tagaacgcca aaaacaaatc gaactggaag
    14101 aaaaagaaat cacacgtcgt gaaaagcaat atgattcgga agtgaagaaa aaagcagatg
    14161 ccgatcggta cgctaaagaa caagaagcac aagctcaaaa agtcaaagaa gtgactgaag
    14221 ccgaagcaga acgattccgt gtggaagcac tggcagaagc ggaagccaat aagacacgtt
    14281 tagctggaca agcagaagcc gaagcagcac tagcaaaagg aaaagcagaa gccgaagcaa
    14341 aacaaaagat cgccaatgcc ttcaaggaat atggcgaagc agctgtcttg agtatggtta
    14401 tcgatatgct accgcaattg atgcgcgaag cagcacagcc gctaggaaat atcgaaaaaa
    14461 tctctgttgt cgatacagga agcagttcag gagaaaccag tggagcgaac cgagtgacaa
    14521 attatgccac aaatttgctt tcttctactc aagaaacact aaaagcgaca acaggacttg
    14581 acttgaagga aatcgtagag aatttgtcga cacgtgggac tactagccaa gtaaatatgt
    14641 atgaagcgcc aaaagaggca gaacaaaact cgatgaagga agaataaagt agtacacttc
    14701 accgttcaat caaaaatgga ggaagacttt ttaaagttct tctctccatt tttttatttt
    14761 attgcaaaag aaaattagtc ctcgctattt ttcttttgtt tttggatctt agcttctatc
    14821 tcttgtaaat ttgttcttag caatcgttct ttcttttgga gttcgaccaa atcttctgat
    14881 agttcttcct tcttttctgt ttcaggagga cgaagaaaga aattgatacc gattgcaatg
    14941 aatgtcccta taaaagtatc tagtacacgt tggatagcat acaaagcgga ttcgccttga
    15001 gggatgctca acgcaatcag caacaacgta gctattgcgg aaatgatacc ggaattattg
    15061 ttaataccat ctgacacgac gatcacaata ataactagaa taggaagcaa gatcaattct
    15121 accaaaaaat cattgtgaaa atagcttttg acaaaaaaat aaatgatagc taatgtacca
    15181 cctaatgtat tgccgatgat cctagacttt ccaaaagaaa cggttgtagt caagtcctga
    15241 cgcaaagaaa aaacagcaga taatgcagca atcatcggga ttcctcgttg agtcaaatga
    15301 aaaaacaaga tacataaaaa aacagctaag gcacttttga tcgtacgcat acctagtcga
    15361 aaacggccaa attgcataga gattctcctt tttggtcttt ttactatcat acctaaactt
    15421 ttgtctgata gctagttttt cattattcaa gtataattaa aataaaaaag ggggaagtaa
    15481 atgcgtatag gaatgcgaac agtcaaaaca gtgatcagtg tcttcttttc gatggtcaca
    15541 gcgggtagct tatcgctgtt gtattggcca gctgcaggga tcattgcatt attaagtgta
    15601 ggaaatacca agcgctctac attgcgtacg ggcatgaatc gaatcgcagc ctttatatta
    15661 gctacggcaa cagcactgtt cagttttttt gctgttgggt atactatttt tggcttcagt
    15721 ctttttttat tcctgttcat cccgcttgcc tctcgcttca aattaaccga agggatagta
    15781 gtaaattcgg ttttggtcac tcattattta gtggaggaaa acatgtcttg gcagctgatc
    15841 ggcaatgaag cgggattaat ggcgattgga ttagtatttg ctttgcttgc aaatgtttac
    15901 atgccagatc gtacgaagca gctgaaggaa aaccaaatac agatcgaaaa agaatttcga
    15961 aatattttga gacaaatggc agaatttctt ttatcagaaa ataaagaaga tgtgcagatc
    16021 aaatgcgaac atttactgac attcattcgc gaaagccaag aagatgccag agaacatcaa
    16081 gaaaactact ggctcagaca gccgctatac tacgaaactt acttctctat gagaagagca
    16141 caggccaatg tgattaaaga catgctggaa aatctagaga gaatccagca accagcttat
    16201 tatggaaaac atatctatgg tttattgatt tatactgcag aaacattttc agagtcaaat
    16261 gatggcagac aaattttaac acgtattgaa gaagtttatg tattgtatcg gcaaatgcct
    16321 ctgccgacta gtcgccctga attcgaggat cgagcagaat tgttccagtt tttgcagtct
    16381 tttaaaagtt ttattgaaat caaggcagaa ttctcccaac aaaaaatcca aaaatagagg
    16441 ttgtgaattc agctaatatt caggtcaaaa agctgatttc acaacctctt ctttaaacgg
    16501 tgttctcctg tcaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgtt
    16561 ttttgaaatt gctccttgtg actcggagct gaacggtagg ctcacaacct ctttcttaaa
    16621 cggtgttcaa aagctttcct ctgcggtaat aagtccagcc aaacgaaaaa taggaggagc
    16681 tatttttgtt tggctgttct tattacttga tgcagaacag ctttttcaca accttttaaa
    16741 attacatatt tttccataga acttgcattt gatccaagat ttcgatgaca tcttttgtca
    16801 agaagagaga gcgattgggc aagtcacctt caatcatgtg taccatattc tttatttcgt
    16861 aattcattgc ttcagatgta ttcccgcttt cgatgaattc ctttgtacca tctgtaaata
    16921 agatctccgc acgatcagca cgcgggtaat cagcaatcgt cacataaccg ttctcaaagg
    16981 caacgatacc gacttttggc atttttgcct gaaaagtcaa gctgacagca gcaagttcat
    17041 tttctttgtt acgcaagata gtgacggatt gttcgtccac gcctgtttca aaaggaacca
    17101 ttgtagaggc aactacttca ggttgagagc tcaaaaagaa acgagcgaaa gatacagcat
    17161 acgtaccgat atccaacaga gctcctccag ctaattctgg attgaagaac cggtttttag
    17221 ggtctggttc tttgtaactg ccgaaaggtg cttggatcat ttttaaagcc cccagttttc
    17281 ctgtatccat tattgagcgt aactgctgat aaagtggcat gttgaagatt gtcatcgctt
    17341 ctgctaagat caaattcttt tcttctgcta agcgcatagc gtcagccagt tcttttttat
    17401 tcatcgtgat ggctttttca catagaacgt gtttgcctgc ttccaatgct gccaagatat
    17461 ggtcgatatg ctgtctattt ggtacagcaa tgtacacgat gtcgatttca gcatcgtcta
    17521 acatttcttg atagcttccg taagcttttg gaatattgta acggtgagcg aagttttccg
    17581 ctttttctga agttctggaa gcaactgctg ttagttcgct cgtctcttga ttgaagcttt
    17641 cggcaaactc atgtgcgatg gtaccaagac caacgattcc ccattgatat tttttcatct
    17701 gaaaacgctc cttcatagtt ggattgagtt tcttgtctgt ctattattat aacaaaaatc
    17761 acgaagaata aagaggagag gacttgaaac attaaaaaaa atgaaagcag cgatttaaga
    17821 ggtgcttttt gatcaaaact taagtagaat agttaaaagg aggggataaa cgtgcatcaa
    17881 gtatatattg atcttattat gagtgtatta ttagaacagt ttgaaactga aaaaaatttt
    17941 ttagatgaat acttgctgat cagtccagaa caatggcaaa aatggaaaga tggcgagaaa
    18001 aatctgccat ctgaagcgat gcagaaagta aaaaatcttt tcactgatta tgaatggatg
    18061 ctaacgcaaa agattttgcg tcaaacgatt atttttcccg aaaaacgaaa cgttgccgtt
    18121 gcagagtaca agcaaatcaa aacacgcatc gcgcaaaaat ggcttagcag caaagcagca
    18181 aaagtcgagc tgatcccttt gaaaaataat cagcaggctg gtagatacct tgacttgaaa
    18241 gtaagtattc aatatgatga atggggctat gatgatattt tgaattttcg tctgccagca
    18301 gatgttcaaa aacagatcga aggagaaaaa atcgaacttc tcgattgggt gaatgaaaat
    18361 ctggaagaaa cttacgttca tgaggcgaat ggggaacgtg aagaacaacg aaaggatttt
    18421 tcaagtgaat aaatttttag gatttggagc agttgtgatt atgatcgcag caatgggcta
    18481 tgttattttt agcgatgatg attcgcatgt tcaagccggc aatcaagcag tcgaacgaac
    18541 aagcaccaaa acaactgcta gtaaagaaaa gaacagtcaa acaaccagca gcaaggtaaa
    18601 ggacaaagaa acaaaagatc gtacgtccaa acaattgcca gatgttaaaa cagacgattg
    18661 gaagctagtt ttggttggtc cagatcacaa attagaaaaa gaaattgatg agtctactca
    18721 attggcatca ttaagtaacg gatacttgat cgacaaacgc attgctccag aatatgaagc
    18781 atttgccaag gctgctgaag cagctggatt tccattagtg atggtctcag cttaccggtc
    18841 tgtatcatcc caacaacaag ttttcgaaca aaatgtccaa gatgtgatga gtcgtcaagg
    18901 tgtatcagaa gaggaggcga caaagattac taaagaaacg attacggagc caggatacag
    18961 cgaacaccac acaggtctgg cagtcgatgt cgtcgatcaa aactggtaca acagttatcc
    19021 ttctactgta ttggatgcaa gttacggaga tcagcctgga gctaaatgga tcgcagataa
    19081 tgctgcgcag tatggattta tcgttcgtta tccaaaagga cacgaagata taactaagat
    19141 cacatatgaa ccttggcact tacgttatgt cggtaaagaa aacgctgagt atatcaccaa
    19201 acaccaattg acgctagaag aatatctgaa tgaattaaat gaaaagtagg aattgatcat
    19261 ggcgaaaaaa aagtggaaaa agcaaaagcg caagaatatc cgttggccag cagtggttgc
    19321 cggtgtagca gtgatactct tagcctttgt tttttccttg aagaatctgt cttctccttt
    19381 tactgataat cagcaagaaa atgaagcgca atcccatcag caatttattg aacggcttgt
    19441 tccgcatgcc caagaactac aagatggtta tggagtgctt cctagtatca ttttaggaca
    19501 agctatatta gaatcaaatt ggggaaaaag taccttggct agcaaataca ataatctttt
    19561 cgggatcaag gcttatgggg atcagaagaa agtaagcctg gaaacaaagg aatttgttaa
    19621 cgaagaatgg ataactatcc aaggagactt caaagtgtat gattcatggg aacagtccat
    19681 ggatgaccat acacaactgt tcgtccaagg agtagattgg aatcctgcgc tgtatgaaaa
    19741 agtgattacg gccaccaact atcaggaagc cgcacaagca ttgcaagatg caggttatgc
    19801 gacagacccc ggttatgcgc aaaaaatcat ccaggtcatc gagacgtatc aattgaatca
    19861 gtatgaccgg agatagcaaa ggagaagtgg ctgatggcag aacaatttac gccagaaatc
    19921 atgacggaat tgagaaaaga tatcgtaaga gtaccggaaa tcattttaga agcaagcggg
    19981 atcaagatat ttggaaaatt gatccgttcg atcatcttta ctacggatat tgcgatcata
    20041 cgtaatacaa atgcgaatgc agtaattgct gtgtatcctt ttacacctca tccagcgatt
    20101 actaaaagca ttattgaggc agctgacatt ccagttttct ccggtgtagg tggtggtttg
    20161 acacaaggtg ctcgttcttc ctacatgagc atgttcgcag aagcgcaagg ttcgttaggg
    20221 gtcgttttga acggtcctac gcctttagag acagtaaaag aagtttgtcg agtagttgat
    20281 attccagtta tcagtacggt aacgtcgaaa tatacagaaa tagatgaaaa gttagcagct
    20341 ggtatttcga ttatcaatat cagtgcaggt aaagaaactg cagaagcagt acgccatttt
    20401 cgtaaaaaat atcctgaact tcctatcatt gcgacaggtg gtccaacgga agaaagtatt
    20461 gctgagacca ttcaagcagg agcaaatgcc atcacatata cgccaccaag tagcggagag
    20521 ctttttagtc gtaaaatgga aaaatatcgg gaaactgaga aaaagaatca tgaataacag
    20581 gtgagaatca aaagacgagg aagagcagat agtggctctt cctcgtcttt tgtactccag
    20641 tactatttgt tcagaattca aaaaaaagag aaattttttt gtttttttac ctgtaatgta
    20701 cataagaaga gttaaaacct tttgttatta ttagcattct aacaaaaaaa tgtcttgatt
    20761 tattgaagcg ctttctatag aatgaaaaac gaataaaaac taaaaaaggc acccgacaat
    20821 ttagtagcag ctaaattgtc ggggcttgaa aagtcagcgg gaactgacca atcaagttgc
    20881 catagtcaat gcttgtgcat cgacgtgtat attgtaccta atttcataca gaattactag
    20941 ttatttttct gttttttagg gaagagcgca cacgaagaag catgcttatt tagctatgtc
    21001 aaggatattg ggcagttgtc tgatatcctt ttttctttgc gtaattatcc tcccatcatt
    21061 gaagcaagaa gtgtttttag cgatgaaaga agaatcagca ctatctaatt tttcttgaaa
    21121 gtattgagtt gttcatccta ggcagaacga taaggcaagt cttggttttt attccccttg
    21181 aaaaagatcg tttaataaat atgttctgta taaaaataca gtatattgaa aaataacaat
    21241 ggaagtagag gtgcagagaa tgaagaaaag aatgagaatc atcttagctt taagtgttat
    21301 gttattcttc ttgacggctt gtgggcaaga agatgacaaa aaaatcacgc tgaacatggc
    21361 ttggtggggt tcgcaagtcc gacacgatgc aacagtaaaa gtaatcgagc tttatgaaga
    21421 aaaaaatccc catgtgacga tcgattatga attttatgat tatgaaggat attacacaaa
    21481 attaggtaca ctagcagcat cagatgatat ctgggacatt ttccaactga acaatcaatt
    21541 tcctcaatac atcaatcaga ttgaaccatt agatgaatat gtggagcaag ggataattga
    21601 tatttctaat gcagatgaat cctatttagc aacgacgact tacgacgggc aattagttgg
    21661 actgtctaac ggggtgaatt cgtttgcgat tgcttacaat aaaaatatat ttgaacaatt
    21721 aaatatccct gaaccagctt caaattggac atgggatgaa tttgaacaaa tcagctatca
    21781 aatcactgaa gaattaggaa tctatgctat ttcaagattc gaagatttta tagctggctg
    21841 tattattcaa ataccacaag ttgagaaagg attgaatttt tataatcggg aagatctatc
    21901 taacagcttg ggatttgaaa atccagacta tctaacagat tttttccagc tgcgtaaaaa
    21961 tctggtagac aaaggggccc atccagaacc aggtggtgga gctgcaagta cgacggatct
    22021 gtcttttcag gcggttaggg atagagaagc ggcaatggtg tttctatcaa gtaaccagtt
    22081 gacagaaata ttgtcagggg ctcctgaaga tatggagatt cagttgatta atccacctcg
    22141 cagaaaagca gatggagaat ctggtatccc tttacgttct tcgcaaatgc tgagtatggc
    22201 aaaaagctca gcccataaag aagaagctgc taagtttatt gatttctttc aaaacagtga
    22261 agaagccaat gaaattttaa aaggagaacg tggtgtacca atcatgtcga atattcgaga
    22321 acagctcgct cgtgcagatg atgcaacttt gcaagcgaca tttgattatt tagaaatgat
    22381 ttccgatatg gacaacggcg aagtgaatgt ctttgatagt gctttgaatc cggaaattca
    22441 agaccaatac aatcttttag ttgaaaaagt catttatgaa gaagtaacag cagaaggaag
    22501 gcatgaaaaa agaaaaacta aaacggttct tggatcaaga caatgtggtc ggttacgtat
    22561 ttgcttctcc attcatccta ggatttctat tgataacagt gataccgatg atcttgtcac
    22621 tttattattc gttcaccgat tatcgtttgg gaaatccagt ctcatggatc ggcttggata
    22681 attatataaa gatgctgagt gatgcccgtt ttttgaactc catccgagtc acttttctct
    22741 atgtatttgc atctgtacct gtgaaactga tctttggtct attgattgct tatcttttaa
    22801 cgaggaatat caaaggaacg actttctttc gttctttgta ttatgtgcct agtttgatcg
    22861 gtggcagtat ttctatcgct ctagtttggc gggagttgtt ttctaaaaac gggttagtga
    22921 atattttcct ggaacgatta ggcgcacctg cgctttcctg gtttagcgat caaaaactgg
    22981 tcatgatccc tcttgttttg atgtctgctt ggcaattcgg ttcggcaatg atcatctttg
    23041 ctgcaggtat caaagagatc ccaggctcgt attatgaagc ggcagagatc gatggagcga
    23101 acaaatggca gacgttttac aagatcacgt tacctcttct ttcgccgatc attttgtata
    23161 acttggttat gcagacaatc gcggcgttta tgacttttac gcaggcattt atcattaccc
    23221 aaggtggtcc gaatgatgcc acgaactttt atgctcttta tgtgtataac caagcttttt
    23281 cttttaattc tatggggtat gctagtgcta tttcgtggat catgctgttg attatgggag
    23341 ctgtcacctt tctgattttc aaatcatcaa aaaaatgggt cgttgtgtga atgagaggag
    23401 gaagaaatga ataatacagt aacgaaatgg tttcaatacg gttttatttt gctattaggt
    23461 ctagtcatga tttatccggt cttatggatg atcgcaggtt cttttaaaac ggatcaagag
    23521 attatgagtg gttcgctgaa tttgattcca gagacttttc aatgggaaaa ttacgcaaaa
    23581 ggatgggcgg ggtttgctgg aatttcattt tttactttct ttaggaattc gtttgtcatc
    23641 tctgcagttt cgacgatagg gacagttctg agctcgactt gtattgctta cgcattatcg
    23701 agaatcgatt tcaaaggaaa aagattctgg ttcgtcgtca tgttagtaac gatgatgatt
    23761 cctgcacaag tgattttgat tccgcagttt attatttaca atcggctgaa tttggttggg
    23821 acgtatgtgc cgctgatttt accacatttt tttggacaag catttttcat ttaccagatc
    23881 atgcaattca tggtcagcat ccccaaagag ttagatgaat cagcgattat tgacggatgc
    23941 agcaagtata gtgtctttac caaaattatt tttcctttgc tgaaaccttc gatcatcacg
    24001 acgatcatca tccagtttta ttggaaatgg gacgacttca tgggaccatt gatttattta
    24061 aacaagccga gatcttacac ggtatcgatt gccatcaaat tatttgcaga cgctggatct
    24121 agtacgactt acgcgagtat gtttgcgatg tctacgttgt cgctgctacc ggtttttctg
    24181 attttcttat tcttcaataa atatcttgtc caggggatca gtacaagcgg tctgaagggc
    24241 tgaatatgaa aaagccaata gaagagatgt ttctttacaa gctcaactac tatgtctgtc
    24301 ggattttttt aaccagtctt atcagtaatg ctcttttcgc tttagttcta ggatgtttgt
    24361 tcactttagg attagtgctg tctgctcgtg ttgtctatct gatttatttt ttgatgatgt
    24421 atccttctta ttatctatct gtccattact tggcagaaaa aaaggtatgt tggaggaaag
    24481 ggaaaatcaa acggatgtgt ttcatcatta cgttgtttca gctgtttgtc attatttttg
    24541 cagttgattg gtatttatta gtgggaaaga ttggagcttg gtatctgatt ccttttctat
    24601 tgtgtctgtt gctttttgta tcccatagtt tttttagtac gctaattgat atctgcaaag
    24661 aagataaagc agatagtctt gtttatttga tcaaaaggaa tatctggctg ttgtcaaaaa
    24721 acgtcaaggg gtttctgtgg tatttatgcc ttttgtatgg actgttctgg tgtatacagc
    24781 ctgatttttt actcactgca ttactgtatc cagggctgcc atttgtcaca gtattttgct
    24841 taaaagaaaa ggagaatgac tttaaatgaa gaaaaaatat gtacaagtcg gacttggtgg
    24901 gagagcgcgt tttttttatg aggcaattgc ctctacgtat catcaaactt gtgaactagt
    24961 cggtttttgt gatgtatctc aaacaaggat ggattatgca aatcggatac ttgtgaacaa
    25021 gtatggacag aaaaaagtaa agacatgtat gccatcacaa tttgagcaac tactggatga
    25081 cgtacggccg gctgaagtca ttattacgtc agtagatcga acgcatcatg attatatcat
    25141 tcgtgcaatg aaaaaaggat atgatgttat ttgcgaaaaa cctctgacta tcgatgagga
    25201 aaaagctcaa gaaataataa cggtccaaaa acagacggga agaagcctgc gagtcacttt
    25261 taattatcgt tatgcaccgt atcacacgaa gataaaggaa ctgatagaaa gtgatgtgat
    25321 tggtgatgtg ttttctatcc acttcgaatg gctgttaaat acgcaacacg gggcagatta
    25381 tttcagaaga tggcatcgaa ataagcacaa tagcggcggg cttcttgttc ataaatctac
    25441 ccatcatttt gatctagtga atttttggtt gaacagccgt ccccaaaagg tatttgcatt
    25501 cggtggatta aacttttatg ggatgcataa tgcggagaaa cgcggagtag aaatatttta
    25561 cagtcgatgc tatggaagcg aactagctaa agctgatcca tttgctatag acctgtcagc
    25621 caatgaggaa ttgcgagaaa tgtatttaga agcagaaaaa gacagtggat atattcgtga
    25681 ccgcagtgta ttttccgatg atatctctat tgaagataca ctaggcgtaa cggtcaaata
    25741 tgaaactggt gcgattttga cgtattcgct aaacgcgtat atgccgtggg aaggattcaa
    25801 tgtttctttc aatggcagca aaggacggat cgaaatgaaa gtgatcgaaa agtcttacat
    25861 caatgcaggt ggagataaaa aggatgaagg agcagccgtt cttcagtcca tccatgtcta
    25921 tccgatgttc ggtgagccat atgaggtaat cgtcgaacaa gaagcgggcg gacatggcgg
    25981 aggagatccg cgtttattag atgatctatt tggtgaagaa aaagaagaag atccgtggaa
    26041 tcgggcagca actcacgtag atggtatttt gtcgattttg acaggaatcg cagcaaatca
    26101 ttctattgct tcaggaaaag cagtagtgat tgacgagctt gtcagcttaa aagaataaga
    26161 atagatttac ggaaattact tcccgtagtt tataaaatct tgccttattg ccgataggct
    26221 gtcttttgaa cactatttat tcggaaaaag gggctatgac atgctttttg tcatgccttc
    26281 tttttatgtg ggctgctttt ttagatagga gaaaataaaa ataactattt aaacccgaac
    26341 aaaaaggaat aatgcattaa aaatcattag attttacaaa aaaataagta aaccacgttg
    26401 tcagtttcag aaagtagtgt tagactgttt tcaatatttg aaacaacatt ttttattcaa
    26461 atatggcaaa ctgccttcac gtgcaaaaca gttgcaagaa aatgaattta cactattatt
    26521 agttttacat acctacttat atatcgtcat aatatggatg acagtttcta gccagtaccg
    26581 taaatgctgg actataagta aaagattggc tatttcaccc ttcttttctt atgataaagt
    26641 ggagggtgtt tttctttaac agaaacatag taggtttttg cacaaaagcg gttatttcgg
    26701 tattaataaa tgataaagga gcaaataaag tgaaagaatt agttgaacgt attcaaaaag
    26761 acggacgtat attaggcgag ggcgtattaa aagtcgacag cttcgtcact caccaagtag
    26821 accctgtatt gatggaacaa atgggaaaac ggtttgcaga agtatttaaa gaacaaggaa
    26881 tcacgaaagt tgtgaccatt gaagcttctg ggatcgcacc agctttattt gctgcaagag
    26941 aattagatgt tccaatgatc ttcgctagaa aagcaaaaag tttgacgatg gatgaagaat
    27001 tattgacagc ttctgtttat tcttttacga aacaagtgac aagtacgatt tctatttctc
    27061 gtaaattttt atcttcagaa gacaaagtat tgattatcga cgatttcttg gcaaacggac
    27121 aagcggcaaa aggattgatc gaattatgcc agcaagctgg agcgcaagta gaaggaatcg
    27181 ggatcgtcat cgaaaagtct ttccaagatg gacgtcagtt acttgaagaa atgggattga
    27241 gagtcgtttc tcttgcgcgt attgcttcct tgacgaatgg acaagttgag tttcttgagg
    27301 aggacgcata atcgtggaaa aagaaacaca aaatggaaaa gctgcggttt tagggctgca
    27361 gcatctgcta gcgatgtatg caggagcagt agcggtgcct cttttgatcg gtacaggact
    27421 aggctttaat caagaacaga tgacttattt gatttcaatt gatatcttta tgtgtggatt
    27481 agcgacatta ttgcaattga cagttaaccg atttttcgga attggcttgc ctgtcgtttt
    27541 aggatgtgcg atccaagcag tagctccact gatcttgatc ggaaccaatg aaggagtagg
    27601 agctatctac ggatctatta tcgcttctgg gatctttgtt gtgttagttt ctggtgtctt
    27661 ttctaaaatt aaaaaactat tccctcctat agtaacagga acggttatca ccgttattgg
    27721 tttgactttg attcctgtag caatcgagaa aatgggtgga ggtaatgcaa cagcagctga
    27781 ttttggtgac aaaacaaatc tgctacttgc atttgtgacg attctcctaa ttgtaggcgt
    27841 gcaaatgctg gcaaaagggt tcattcgttc aatcgcggta ttgattggtt tagtcggagg
    27901 aagcatctta gctgctttca tgggaatgat tgatctgagc gcgattggag atgcgccgtt
    27961 gttccatgtt ccacaaccct tttattttgg taaaccaact ttcgacgtat ggtcgatttt
    28021 actaatgatc atcatttcca tggtcagcat ggtagaatca acaggtgtat attttgcact
    28081 tggtgacatt actgggaaaa atgttggaga agaagaattg aaaaagggat atcgtgcaga
    28141 aggacttgcc gttatcttag gcggtatttt caatacattc ccttatactg gcttttctca
    28201 aaatgtcgga ttggttcaac tttcgggaat caaaacaaga aaacccatct atttctctgc
    28261 attcttcttg attgttttag gattattccc taaaatcggt gcgttagcac aaatcatccc
    28321 tgagccggta cttggtggag gaatgttagt catgttcggg atggtggcag tccaaggaat
    28381 ccgtatgctg ttggaagttg atttcagcaa tgataaaaat ctattgattg ttgctgtttc
    28441 tgttggattt gggctgggtt tcaatgttac gccgacatta ttcagtcaaa tgccgcaaac
    28501 attgcaaatg ttcactggaa atggcatcgt aatgagtagc attacggcta tcatattgaa
    28561 tttgatcttt aatggattga aaaaagatga agaaatattg gaagaagcct tcaagaagta
    28621 atagagcaaa agaaaggttg tgaaatcagc gttttgacct gattgttaga gaaacaaacg
    28681 gaaaaatagt tcctcgtatt ttgccgtttg tttgagctaa taccgagggt cagctgattg
    28741 aacaccgttt atccataaaa aggaatgtga cctaactttt gtcacattcc tttttttcat
    28801 ctttaataaa cacgaacaat aaaatgtttt tctttgttaa cattcgaaat ttctttgaca
    28861 tcaaaaaaag tgattgttaa aatatgaatg tagaaaaact gggaggaaaa acaatgacag
    28921 caaaaatctc tgtgattatg gggagtactt cagattggga aacaatgaaa gaagcatgtc
    28981 aaatattgga tgaattcggt gtgtcttatg aaaaaaaggt cgtttctgct catcgaaccc
    29041 cagatttgat gtttgaatat gcacaaaacg ctcgaaaaaa aggaatcaaa atcattattg
    29101 caggagcagg gggagccgcg catctgccgg gtatggtcgc atcaaaaaca actttgccgg
    29161 ttatcggtgt accggtccaa tcacgaacat taaatggttt ggattcgctt ctttcgattg
    29221 tccaaatgcc tggaggagta cctgtagcta ctacagcaat cggaaaagcg ggagcaatta
    29281 atgctggact acttgcgata cagatgcttt ccatgtatga tttagaattg gaggctaaat
    29341 tggctgaaag acgttcaatg ttggctaaaa ctgttataga aagtagtgat caacttggtt
    29401 aaacctttgt tgcctggttc aacgatcggg atcgtcggtg gcggtcaatt aggtcgtatg
    29461 atggcgatta gtgcaaaaga aatgggcttt aaagtaggtg tgctggatcc tgtgagcgat
    29521 tgtccagccg cacaagttgc agattggcac attgaaagtg tctatgatga cacctttgcg
    29581 ctgaaagagc ttgccagaag aacagatgtc attacctatg aattcgaaaa cgtcagtgta
    29641 gaggcgttaa atgcgatttt acccatgtca tttattcctc aagggacaga cttgcttgcg
    29701 attacgcaag accgcctgtt agaaaaatct tttttggaaa cgaataacat cgtgattgct
    29761 ccttatgcaa cgatcgtcag tccgacggat atccaagatg cgattgacgg gatcggctat
    29821 ccttgtgttt tgaaaaccac tcgaggcggc tatgatggca aggggcagta tgttttgaaa
    29881 agtcgggcgg atctagctcc tgccatggac cttttaagag aaggaacctg tgttttagaa
    29941 gcatggatcc cgtttgaaaa agagatttcg atcatggtgg caggaaatgg tcaaggagat
    30001 ttcacgactt ttccggtcgt ggaaaacatc caccacaaca atattcttca tgaaacgatt
    30061 gctccagccg cgattgatca ggatgtgatc gaggaggctg agcgtattgc ccgtgtgatt
    30121 gcagaagcag tgagcctttc aggtgtttta ggggtagaaa tgtttttaac aaaaacaggc
    30181 gggctatatg tgaatgaatt agctcctcgt cctcacaatt ctggacatta ttctattgaa
    30241 gcttgttcta tgagtcagtt cgatgcccat attcgaggta tttgtggttg gccattagga
    30301 gaaaaagtac gtttgataag caatgcagta atggtcaatg ttttgggaga acaactgctg
    30361 gaaagctatc gcttgattga gaataagcca gactggcaat tccatttcta tggaaaaaaa
    30421 caagcaaaaa aaggtcgcaa gatgggacat atcactatct tgacagaaga catccagcaa
    30481 acgctggaag aaatcaaaca aacgaatatt tgggattaga ggagaaaaaa catgttagaa
    30541 cgttatacac gccctgaaat ggggaatatc tggacggaag agaacaaata ccgtgcgtgg
    30601 ctcgaagtcg agattttagc agatgaagct tgggcagaac ttggtgaaat cccgcaagaa
    30661 gatgtgaaaa aaattcgtga gcatgcatca tttgatatcg atcggatctt ggaaatcgaa
    30721 aaattaacac gccatgatgt cgttgcgttt acacgagcgg tttctgaaac attgggagaa
    30781 gaaagaaaat gggtccatta tggtctaaca agtacggatg tcgtagatac tgcttatggc
    30841 tatctcatca agcaagcaaa tgatatttta cgggaagact tgaaacgttt tacggaaatc
    30901 attggcgaaa aagcaaaaga acacaaatat acagtgatga tgggtcggac acacggtgtc
    30961 catgctgaac caactacttt tggattgaaa cttgccacat ggtattcaga aatgaaacgg
    31021 aacatcgaac gatttgaaca tgcggcaaaa ggagtagaag caggaaaaat ctcaggagca
    31081 gtgggaacgt tcgcaaatat cccgccattt attgaagagt atgtatgtaa acaattgggg
    31141 atccgtccac aagaaatctc tactcaagtt ctccctcggg atctacatgc tgaatatttc
    31201 tcagcgatgg cgcttattgc tacaagtatt gaacgttttg caacagagat acgcggattg
    31261 caaaaatccg aaacacgtga agtagaagaa ttttttgcga aaggtcaaaa aggttcttct
    31321 gctatgccgc acaaaaggaa tccgatcggt tcagaaaata tggctggtct agccagagtg
    31381 gtgcgcggac acatggtcac tgcctatgaa aatgtcacat tatggcatga aagagatatt
    31441 tctcattctt cagcagaacg aatcatcttg cctgatacca cgattttatt gaattacatg
    31501 ttaaatcgtt ttggcaatat cgttaaaaat ctaacggtct ttcctgaaaa catgaagcgg
    31561 aatatgtctg caacatttgg tttgatttat agccagcgcg ttatgctgaa attgatcgat
    31621 aagggaatga caagagaaga agcttatgat cttgttcagc caaaaactgc gtacgcttgg
    31681 gacaaccaag tgcagttccg cccgctgtta gaagaagatc caaccatcac atcggtatta
    31741 tctaaagaag aaatagacga tgcctttgat tatcatcatc atttgaaaaa cgttgacgtg
    31801 atattcaaac gagtaggttt ggaataaaga agagtgagtt gcaaaattca attaaaatag
    31861 aatagcaaaa ataaccaaaa aagaagcaga tccgttagcg gttctgcttc ttttagttta
    31921 ttatattttc ttatcaataa taggaacctg attttaaaag cggctacctc tttaccgtgc
    31981 ctctttacat acaaaaaata gaggaattaa gcgtaaatgg cttggctatt ataactaaaa
    32041 tgctggttat ggagatgttg cagcaagttt tcgagaaaag cgtattccat ctcttgtcca
    32101 ttgaataaga tctggatacg ataatgattt ttgaaacgct tctgttcatg aatctgactg
    32161 attgaaagtt cgcctttgtg ttctgtctga taaagaaaag tccttttacg gcattcagca
    32221 cagcgcttgg ttgctcggcg gatttttcgg aatgtataaa aactagtatc tttattgcat
    32281 aaataaacgt ctttgatcga cagcaaatca ttatatagac aggaacgttc ttcagctgtt
    32341 aattctgata aaaaaagctg accttgtttt attgcatctt ccatcggcaa gtgcctcctc
    32401 gaacccatta tccaaatttt tagttaattc taatatacca tcattaagta taaatgtaaa
    32461 cgcttgttga tggtataaga ttttcgtttc tgataaaaca cgaacattaa aagaatctaa
    32521 taccttaaat gtacatattt ttgttgattt ataaaaagat gattgctaaa atttaaagtg
    32581 aaaaaacgaa caaaaaacga tgtatttgct tttaatgtaa ataaaaagga gagacgtaca
    32641 tggagaagaa agaacttttg tatgaaggaa aagccaaaaa aatatatctt acttctgatg
    32701 agaacttgat ttggatagaa tatttagatc aagcaactgc gttgaatggc gcgaggaaag
    32761 accagataag tggaaaagga gaattgaata atcaaatcac aagtgcaatt ttccgttatc
    32821 tgactcaaag aggaataaaa aatcatttta ttgaacaatt gtctaaaacc gaacaattag
    32881 caaaaaaagt acaaattatc cctctggaag tggttatccg aaatattgca gcaggaagtt
    32941 tctcaaaacg tctaggtgtt cccgaaggta aagaattcat caaaccaatc attgaattct
    33001 actataagga tgaccagttg gatgatccat ttatcaatga agagcatatc tatttcttac
    33061 aacttgcaaa accggaagaa atcagtgagc tgaaagccca agcgttggct attaatgaaa
    33121 ttttgaccga attatttgct aaggtcggtt tggttttagt agatttcaag ctagaatttg
    33181 gccggtcgaa tggacaaatt ttgcttgcgg atgaagtaac accggatact tgccgattat
    33241 gggatgaacg tacgaatgaa catatggaca aagacgttta ccgcagagat ctaggtgatt
    33301 tgatcccagt ctatcaagaa gtatataacc gtctatcgga actaactgaa gaaaaggagc
    33361 gagtatgatg tatttcgtaa aagtatatgt aacctataaa gattcagtgt tagatcctca
    33421 aggggaggca gtaaaaaaag cagtccatcg tatgggatat gaaacaatcg aagatatccg
    33481 aataggaaaa tactttgaga tacaacttgc tgatgcagaa gaaccaatcg atacagtgat
    33541 tgaaacgatt tgtgacaagc tgcttgcaaa tgtaaatatg gaaacatacc gatatgaaat
    33601 caaaaaaatg gaggaagtat aacatgaaat ttgcggtcat cgtttttcca ggttcaaatt
    33661 gtgacgcaga tatgttatgg gcagttcgcg agatcatggg cgctgatgcc gaatttgtac
    33721 ggcatgatga gtcctcttta gaaggatttg atggggtttt actgccggga ggattctcct
    33781 atggtgacta tttgagatgc ggtgcgattg ctcggttttc tacaatcatg gaagaagtga
    33841 ttcgttttgc tgaagaaggg aaaccagtat tcggtacatg caatggattc cagatattga
    33901 cagaagcagg acttttgcca ggtgtactga gacgaaatga gtctttgcat tttatttgca
    33961 aaactgtacc attgaaagtc aaccccaaga cggcctttac ttcagagtat gaagccgacg
    34021 aaataatcta tcttccagtt gctcatgggg aaggaaatta tttctgtgac gaagaaacac
    34081 ttcaagaact aaaaaaaaat aatcagattg cctttactta taatgacaac atcaacggca
    34141 gcgtggaaaa cattgcaggg attacgaaca aagcagggaa tgtgttagga atgatgcctc
    34201 atccagaacg tgcagtagaa gacttgctag gatcagaaga tgggaaacgg ttctttgctt
    34261 ccattttaaa aaacttcgga aaggtgaccg tatagatgaa aacattgaaa gaaccgacag
    34321 ctgaagagat caagaaaaaa agaatctatg ccgaatgggg attgactgat gaagaatatc
    34381 gaatgatcga agaagatatc ttaggaagaa tgcctaatta tacggaaact ggactgtttt
    34441 ctgttatgtg gagcgaacat tgttcctata aaaattctaa accggttcta cgcaaatttc
    34501 ctactagtgg acctcatgtg ttgcaaggcc ctggagaagg cgcaggcatc gtcgacatcg
    34561 gtgatggact agcagttgtc ttcaaagcag aaagtcacaa tcatccttca gccgtggagc
    34621 cttatgaagg agctgcgaca ggagttggcg gaatcatcag agatattttc agcatggggg
    34681 ctcgtccaat tgcgttgcta gattcattga ggttcggcga attagataat ccaagaacga
    34741 aatatttact agaagaagta gttgcaggta tcggcggata cggcaattgt atcggcatac
    34801 cgactgtagg cggcgaaatt gcatttgatc cttgttatga aggaaaccca ttagtcaatg
    34861 caatgtgcgt aggtttgatt gaacacaaac atatccagaa agggcaagcc gctggcgtcg
    34921 gtaattcgat catgtatgta ggtgcaaaaa caggtcgcga cggtatccac ggagcaactt
    34981 ttgcctcaga agaatttaat caagaagaag aacagcaacg ttctgctgta caagtaggcg
    35041 atccttttat ggaaaagctg ctgcttgaag cgtgtttgga attaatcttg catcattcgg
    35101 attctctaat aggtatacag gatatgggag cagcaggact tgtttcatct agtgcggaaa
    35161 tggcttcaaa agctggctca ggacttatat tgacattaga tgaagtccca caaagagaaa
    35221 ctgggatgac accttatgaa atgatgttat cggaatcaca agaacggatg ttgatttgtg
    35281 ttaaatcagg agaggaagaa aaaatccaag aactatttca gaagtatgat ctagatgcag
    35341 tcactatcgg taaagtaaca gacgacgggc aatatcggct atatcatcaa ggagaagaag
    35401 tagcaaatct gccagtcgat gcattagctg aagatgcacc agtttatcat aaagaaatga
    35461 aagaaccagc tcgaatcgta gaatttcagc agttagctcc ttatcagcca gttatcgaag
    35521 aacctggaca agtattgctt gatctgctgc agcaaccgac gattgcttca aaacgttcaa
    35581 tctacgaaac ttatgattca caagtacaaa ccaatacagt tgtccgtcca ggaagcgatg
    35641 ctgctgtgat gagggttaga gggacgaata aagcgttagc tatgaccaca gactgcaacg
    35701 cgcgctatct gtatttggat ccaaaaatcg gaggacaaat agcagtggca gaagctgcta
    35761 gaaatattat tgctagcggt ggtaagccct tggcgatcac cgactgcctg aactatggtt
    35821 ctcctgataa accagaagtt ttttgggaat tatcgacttc agctgacgga attgctgctg
    35881 cgtgtgaaac gttaggaact cctgttattt caggaaatgt ctctttatat aatgaaacgg
    35941 atggtcaagc aatctatcca acaccaatga ttggtatggt cggattgatt gaggatcata
    36001 aacatatcac tactcaggaa ttcaaaaaat caggggatct aatctatata ttaggtaaga
    36061 cgtttgcaga ttttgatggc agtgaattac aaaagatgca gcttggaaga atcgaaggtg
    36121 tgatccgaaa ctttgatcta tccattgaaa agagaaacca agagttagta ttgacagcca
    36181 tccaaaacgg attgattgaa agtgctcatg actgttcaga aggtggcttg gcaattgcac
    36241 tggcagaatc agctttcaaa catcagctag ggatatctgt acagtttgaa ttgtcctctg
    36301 ctcaattatt tgcagaaact caatcaagat ttgtgttgac agtcgcaccg gaaaataaga
    36361 cacgattcga agaaatgatg ggagatgctg ctgttttagc aggtaaggtg acggatgaag
    36421 cgataatcga aatttcagca actgacggtc agatcaaaat agaaactgca gtagcaagaa
    36481 agtgctggga ggatgcaatc gtatgtctta tgaagtgaag agtttaaatg aagaatgcgg
    36541 gatcttcggt gtttggggtc atccagacgc cgctagtgtg gcatacttcg ggctccatag
    36601 tcttcagcat cgaggacaag aaggtgcagg tatcgtgtcc aatgatcgag gaaagcttaa
    36661 ggggcatcga gatttggggc tgctttctga ggtattcaaa gatcaaagaa aattgcagca
    36721 gttaacagga gaagctgcaa tcggacatgt acgttatgca acagcaggca atggaagtgt
    36781 ggacaatatt cagccttttc tattcaaatt tcatgatcag tcgatcggct tagcgcataa
    36841 tggtaacttg atcaatgctc gttcccttag aaaagagtta gaggaaaatg gggcgatctt
    36901 ccattctaac tccgatacgg agatattgat gcatttgatc cgacgaagtg aagaaacaga
    36961 ttttttaaat aaattaaaaa gtgctttgaa tgaagtcaaa ggtggctttg cctatttatt
    37021 aatgacagaa acggcaatga ttgccgcttt ggacccaaat ggttttcgtc cattagcaat
    37081 cggacaaatg aaaaacggag catacgtgat tgcctcggaa acttgtgcat tagaagttat
    37141 tggggcacgt tttataagag atgtccagcc tggtgagata gtgatcatca atgacgaagg
    37201 aatccagatt gattgcttta cgaaggacac gcagctttct atttgttcga tggaatatat
    37261 ctattttgcc agaccagatt caaacatcgc agggatcaat gtgcatactg cacggaaaaa
    37321 tatgggacgg aatttagcga aagaatcgcc agtaaaagca gatatggtcg ttggcgtacc
    37381 taattcttct ttgtcagccg caagcggtta tgcggaagct agtggaatcc cttatgaaat
    37441 cggattagta aaaaatcaat acattgcccg aacatttatc cagccaacgc ctgagctaag
    37501 agagcaaggt gttcgaatga aattatctgc tgtacgagga gtagtggaag gtaaaaaggt
    37561 gattatggtc gatgattcaa ttgttcgggg tacgacaagc agacggatcg tccatctttt
    37621 gaaagaagca ggggcaaaag aagtccatgt gaggattgct tctcctcctt tgaaatatcc
    37681 atgtttttac gggattgata ttcaaacgcg taaagaactc attgcggcaa atcacacgat
    37741 tgatgaaatc aaagaatgta tcggtgcaga ttctttaggt tttttaagtg aagagggtct
    37801 gatcgaatcg atcggtctgg aaaaagatgc tccgtattca ggattgtgta tggcttattt
    37861 caacggagat tatccgacgc cgttatatga ctatgaagaa aaatatcagg aatcactaaa
    37921 agaaaaagta tcgttctttt aaaagcagac caagaaggga gtatatgaat tcaatggcaa
    37981 atgcatacgc aaaagctggc gtagatgtcg aagctggata tgaagtagta gaacggatca
    38041 agaagcatgt caaaaaaacg gaacgtttag gtatgatggg cgctatagga ggattcggcg
    38101 gttgttttga tttatcggtc tttcaattaa aagagccagt ccttgtctcg gggaccgatg
    38161 gcgtggggac gaaattagca gtggcaatcg aagcagacaa acacgataca atcgggatcg
    38221 actgcgtagc gatgtgtgtc aatgatatct tagcacaagg cgcagagccg ttatatttct
    38281 tagactactt agcaacgggg aaaaatgatc cgttacgttt ggaaaatgta gtaaaaggtg
    38341 tagcagatgg atgtgttcaa ggaaatatgg cgctgattgg cggagaaaca gctgaaatgc
    38401 ctggtatgta ctcaggtaat gattatgatc tagccggatt tgctgtaggg atagctgaaa
    38461 aatctcaatt attgacagga gaaacgatca acccaggaga tgtgttgctt ggaatagcat
    38521 ctagcgggat tcattcaaat gggtactctc ttgttcgcaa aattttcttt gagcagcagc
    38581 agttttcttt atcatcaaaa ctgccagaat tgaaaaaaac acttggcgaa gaattgcttg
    38641 agccaacgaa aatctatgtt tcttccttgc ttccgctgat caaagaaaag aaaataaaag
    38701 gagctgcaca tatcactggt ggagggtttg tagaaaatat cccgcgaatg ttaccagaat
    38761 ctatggctgc acaaatcaaa cttggctcat ggccgattct tccaatattc ggctgtttac
    38821 aaaaatatgg tgaaattcct gagatggaga tgtatgaaat cttcaatatg ggcatcggta
    38881 tggtcctggc agtcgaagaa tcgaaagtaa aagaaatagt acacgaaatt gagcaagctg
    38941 gcgaaaaagt ttatgaaatc gggaaagtga tcgccaaaga aaaacatcgt gtggtgttta
    39001 taggagaaga acgatgagaa tcgctgtttt tgcttctgga aacggaagca actttcaagc
    39061 gctagccgat tatctatcga aaaaaggatt ggaatcttcg attgactggt tgttttgtga
    39121 tcagcctgaa gcatatgttt tgaaacgtgc aactgcgttg agtgtaccgg cagattgttt
    39181 ttcacctaaa gaatttgatt caaaaaaaga gtatgaagaa gcaattttgc ataaattgaa
    39241 agaaaaaaag atcgatttga tcgtattagc tggatatatg cgaatcattg gaccggtatt
    39301 gctgaaaaat tatgataaaa gaatcatcaa tatccatccg tctcttttac ctgccttccc
    39361 tggtttgcat ggcatacgtg atgcctttga agctggagta aaagaaacgg gtgtcacaat
    39421 tcattatatc gaccaaggtg tagatactgg accaatcatc cggcaggaaa aagtgagaat
    39481 tgaacaggag gacacgttcg attctttaga agaaaaaatc catagagtag agcatcgaat
    39541 ctatccagaa gtgattagtg aaattattga aaatggaggc tatctgaaat gacaagagca
    39601 ttgatcagtg tatcagataa aactgggatt gtagcattcg cccaaggttt gattgaacaa
    39661 ggagtagaaa tcatttcaac aggaggaaca aagcatgcgt tggataaagc tgggatcaaa
    39721 acgctttcga tcgaagaagt gacagggttt cctgaaatga tggatggccg tgtcaaaaca
    39781 cttcatccta aaatccatgg tggtctttta ggaagacgcg atctccctag ccatatggag
    39841 gcaatggaaa cggaaaatat ccaaccaatc gactttgttt gtgtcaattt atatcctttc
    39901 aaagaaacga ttatgaaacc agatgtcgaa atagcagaag ctattgaaaa cattgatatc
    39961 ggtggtccta gtatgctccg tagtgcagca aaaaaccatg aatttgtcac ctctgttgtt
    40021 gatccatctg actatgaaga agtactttca gaattacgtc agtatgggca tacttcactt
    40081 tctttcagac aaaaactagc ggcaaaagtc tttcgtcata ctgcggcata tgatgcactg
    40141 atcgctcaat acttaacgga ttgggtggaa gaaaaagaac cggaaaaatt gacacttacg
    40201 tatgaacgaa aacaaacgct tcgttacggt gaaaatagcc atcaagccgc tgccttttat
    40261 cagtccgctc ttctggaaaa atattcgatc gcagcagcaa aacaactcca tgggaaagaa
    40321 ctttcttata ataatatccg agatgcagat gctgcgctac ggatcatgaa ggaatatgac
    40381 gagccaacgg tagtcgcctt gaaacacatg aatccttgtg gtattgggac agcatctacg
    40441 attttagaag cgtgggaagc agcgtatgaa gcagatcctg tttcaatctt tggtgggatc
    40501 atcgttttga atcgagaagt agatctgcca acagcagaac agatgcataa gctgtttcta
    40561 gaaatcatca ttgccccttc ttatacacaa gaagctttgg aattactgca aaaaaagaaa
    40621 aacatccgat tactgacagt agactttgcg caggaagaaa atcaaaatcg agaagaaacg
    40681 gtctcagtct taggtggact tcttgtacaa gatcaagatc tggcaataga aaatgaagaa
    40741 gagtggaaag tcgtgacaaa acgtaagccg actccagcag aagcaaaagc attagcgttt
    40801 gcttggagag ctgtcaaaca tgtgaaatct aatgcgatcg tactggcaaa cgaacgtcag
    40861 acagtaggaa tcggagccgg gcagatgaat cgagtcggtt cagtaaaaat tgcgattgaa
    40921 caagcgcaag agaaaatgga aggtgcagtt ttagctagcg atgcttattt tccaatggat
    40981 gattcggttg aatatgcagc caaacatggt ataaaagcaa tcattcagcc aggaggaagc
    41041 atcaaagacc aagattctat tgatatggcg aataaatacg atctagcaat ggtctttact
    41101 gatgttcggc attttagaca ttagagagga gtcggataaa tgaacatttt ggtgatcggt
    41161 gcaggtggtc gtgaacatgc aataagtaaa aaacttttag ccagtccgaa ggtggatacg
    41221 gtgtattgtg cgccaggaaa tccggggatg acaaaagatg gtatcaattg tgtcgctatc
    41281 agtgagcgag atcattcagc gttaatccat tttgttaaga aagaggctat tgattggagt
    41341 atcgttggtc ctgaagttcc tttgttaaat gggatcgtcg atgattttat ggcggaagga
    41401 ttgaaaatct tcggtccgac aaaagcagca gctctgatcg aaggttcgaa aagttttgct
    41461 aaacaattaa tggatactta tcaaatcccg acagctaaat ccagaagttt tgaatgttac
    41521 gaaaaagcag cagcgtatat caaagaaaat ggtgcgccaa tcgtagtgaa agcggacggc
    41581 ttagcagctg ggaaaggtgt agtcgtggct accacagtga aggaagcgct ggatgcggca
    41641 gaacaaatgt tgaaccagca tcgatttggg gatagtagca aaaaaatcgt cgtagaagag
    41701 tttttggcag gagaagaatt ctcccttttg gcgtttgtcc gtaataccaa ggtgtatccg
    41761 atggtcattt cgcaagatca taagcgggca tttgataacg ataaagggcc aaatacaggc
    41821 ggaatgggtg cttattcacc agtaccgcaa atttcaaaaa agatgattga aaaagctgtg
    41881 gaagaagttt taaaaccagc ggctttggga atgaaagaac taggacgctc tttcacgggt
    41941 gttttatatg cgggtttgat tgctacgaag gaaggaccaa aagtgatcga attcaatgcg
    42001 cgtttcggtg atcctgaaac gcaagtcgtt ctttctcggt tgaagagtga ctttgcgcaa
    42061 gtaatcgatg atttattaga gaatcgtaca gtagagttaa agtggcagca agaaggatac
    42121 aatgtaggcg ttgtggttgc agctgccggt taccccgaag aatatgagac aggaatggtt
    42181 ctaccagatt ttctggaaga agagctttct gtttactatg ctggagtttc ctcaaataac
    42241 ggcaaactag ttggagcagg aggacgtctt tatttagtag aagcatacgg tgagacgatc
    42301 aaagaagcac agaaaaaaat ttacaccgcg ttaatggata aaacgacaga tggaacattt
    42361 tatcgtacag atattggaga aaaagctgcc aatagcgaac tgatcgtgaa gtaaatcagc
    42421 tgcttccatt tataaaaaat acaacaaaca taatcggaag cgccgatttt agtgaagcct
    42481 tttcataaag agtgtggtac acttttgttg tgtatataaa taaggaggag ttcagatgaa
    42541 aaaaattcaa ttaggtactt ctgatcagaa agcagctgca gtgatcttag gctgtatgcg
    42601 tatcaacaat gcggaagatc cagtaaaagt gattgagaca gcttatgaaa acggcattga
    42661 tttttttgac catgcagata tttatggaaa cggtgaatgt gaagaaatct ttgcagatgc
    42721 tctagcaaaa acatcaatca aacgagaaga tctttttatt caaacaaaat gtggaatcgt
    42781 tccaggtgtg atgtttgatt tttctaaaaa acatatcatt gaatctgtag aaaaaagttt
    42841 gacccgtctg aaaatggatt atgtggatgc tttacttctt catcgtccag atacattggt
    42901 agaaccagag gaagtagctg aagcgtttga tgaattggaa cggtcaggaa aagttcgtta
    42961 ttttggcgtc agcaatcaaa aaccaatgca aatcgaattg ctgaaaaaat atgttcgtca
    43021 accattagta gctaatcagc tgcaatttgg cttgaaacat actggaatga tcgatcaagg
    43081 gattcatgtc aatatgacag atgaagcaag tatcgatcat gacggtagtg tacttgatta
    43141 ttcccgattg catgatatga cgatccaagc atggtcacca taccagtatg gattttttga
    43201 aggtgtattt atcgggaacg aaaaattccc tgaattaaac caaacattag aagaactagc
    43261 agctaaatat caaacgacac caactggctt ggcttctgct tggattttac gccatccagc
    43321 caatatgcaa gtgatcgccg gtacgatgaa cgtagggcga atcaaggaaa tcgctcaagc
    43381 ttcagaaatt aatatgtctc gggaagattg gtacgaattg taccgtgctg ctggaaacat
    43441 tttaccttaa agattgtcat aagcaggtca aaagggctat aaattaaaaa gacagaataa
    43501 cttgaaagct gttttcagct tcgagatatt ctgtcttttt ttattgcaaa tggaaagagt
    43561 atcattgtac ggatttcgtt tattagttat ttttgttttt aaggaaaagc tgtgtccgat
    43621 attcttttgg agaaatccct tgttttttct taaataaata actgaaatag ttagggtcat
    43681 tataaccaat ttcaaaagca atatctgaaa tacgatagtc tgattgctgc aataattttt
    43741 ttgctaattc gattctttgt tctgtcagat aatcgatgaa tgtttggtta aacgattgag
    43801 aaaaaatcgt actgaaatga gagggactta atgcaacttg ttgagcgacc caagttaagg
    43861 acatatctgg atcggtaaaa ttttctttga taaaatccaa tgcatggttc aaaattggct
    43921 gatatttgtt ccttgaaggc tgtgagggca gagcagttaa gaaagaaatg aacatataaa
    43981 ctgtcgaacg gtaaagcgag tcgtcggcgc tgattgctaa gcgttgactg gtggatgtgc
    44041 aatgaaccat ttcttgcggg attttctttt ctttcaggca gacatgctgg agtagcttag
    44101 tcaagacaaa taaacgatat aatcgtgttc gagttggtgt gccttgtggt ttcgtcaagt
    44161 gttcgatgta cttagatact tcgtttttcg ataatgaaga caaatcaagt gagaggtcaa
    44221 agtaaaagac agaatcaact ggaagtttcg cttcttgaat caaatcctca tagctgataa
    44281 tttgttcttt aagggaactg gcggtttcaa gtatcatctg cgtgatctca aaagatgcag
    44341 gtaattcgct gattctttct gcgacaggac caatggcgac agaaaaatta tttttttgtc
    44401 ccttttctaa ttcatggacc aacgtttggg ctacctgata actggtctcg atcacatttt
    44461 cttcaagaga atcaactatt aaaaatttga tgaatcgaga ggaaacagca gagaagatca
    44521 cttgttgatt ctccctaaat aaaaacttca aataatcact gagatgagcg taatcttcaa
    44581 agtctgtact gacttggttt gctgctaata atacggtcat ttttttccct gctaaagagc
    44641 gattgaaaga ttcagcaagt ttcaatgttt cactgatcgt aagtgagcct ttgaacaaac
    44701 cgttcaagaa atgatttttt ttcaattcga aaacaagatc agaagaagtg tgcgtttctt
    44761 tttgctgcaa atctaaagca gttactgctt tttctattgc ttgtttcaat tcgctacttt
    44821 taatcggttt aagcagataa tcatctgcct gtacctggat tgctcctttc atataatcga
    44881 aatcgtcgaa tccactaata aatatgagac gagtccaagg aagtagtttc cttgcttctt
    44941 ttgaaaaagt caacccgtcc atgaaaggca tttggatatc agtcagaatg atgtcgggct
    45001 gtaagtcgat aattgaagac agtgccatct caccatctcc agcttcgccg acataagtga
    45061 ttggataata agaaccaagg acagttattt gttcccgcaa gttattgcga atgaggtatt
    45121 cgtcttcgat gataaaaact ttatacatgt ttgtttcctc caattgtttt gggagataga
    45181 gggatcatca gtgtgacagt tgttccttct cggtatttgc tgtcaatcgt gataggtttt
    45241 tgatcatttc cgtaataaag aaggagacgc ttgtttacgt tgtaaagtcc gtagccgata
    45301 ctgaaatctg aatcgatccc tttggctaac tctttacgta tttctgccag tttttctgca
    45361 gggatgccaa tcccgttgtc tataactttg aattggatca tctcatcaat tttgcatgcg
    45421 gtaatacgaa taaatccttt gtgacggacg tacttcgttc catggtatac agcattttca
    45481 atcaatggct gcaagatcat ttttaaaata gaaacaggaa aaagtgtttc tggtatatcg
    45541 atcttatagt caagcatatg gccgtatctt atttttaaga tgactagata atcattgata
    45601 tggctaattt ctttttcaag aggaatccag tcttgtcctt tgcttaaaga cgtacgaaaa
    45661 aagccggaaa gtgcatgggt catagtcaga gcttcttcaa agcagtcttg ttcgatcaaa
    45721 gaaataatgg catccaaact gttataaata aaatggggag taatctgagc ttgcaataca
    45781 cgaacttcac tttgtgctaa atggtattgt ttcaaagcgt tctcccgtaa agactggttt
    45841 agatcttccg ccattttatt catgctagcg gtaagtgttt gtaattccag tgtttccggc
    45901 agggttaaac gaaagccaag gttccctgaa gaaagttgtt ctgacatctc gatcagctga
    45961 ttgatcggtt cttggacctt tctctctaaa aagcgacgat tctttttcgc atagaaaaaa
    46021 atggtactaa gaactccaag ttccactatt gttaatagaa tgagcgagcg gacaagtgtc
    46081 gtattttttt tgcttgctaa gtcgatctcc acacgaacaa aatcttgtaa gatatctgac
    46141 agcagttgag tgacagaatc tacctgattg atgatctctt cattggtatc cataaaatct
    46201 tttgccaaga tattattgat cagctcgttt tgataatctt ccagtgtatc gatcgtacgt
    46261 agcgatacat ctaacgtact aatttcttta agtgtatttg tattattttt gatctctcga
    46321 atatcattcc taagttcttc taggatattt tcctcattga actcatctac ggttacttgg
    46381 ccagaaatca agtcccacat actttccaaa actttttgat caaccttcaa agcaattgca
    46441 tctgcttttt ggatatttgc gatcgtctgt tgataaatca aaagctgttg cgaatataac
    46501 agtatgctca gtagcaaagg gataacagca aatagaagca tggctttgtt tgcttgggca
    46561 aaaatctgtt ttatgcttaa attttttgtc atgatacttc tccagtcaat aatttctggt
    46621 tgggatgtta gctatattct gctgggaaaa gacagtctcg gaaataaaaa tatctttagg
    46681 taatgttttt cctgtttgaa tatttccaaa ataacgagta acggtattgg ctacatacca
    46741 gccgacatca ggtttacact cgactacaca attgacgatt cctgagcgta gcttctcgat
    46801 catatcttct tgtccatcaa tagttacaat gatcaaatct tctccaggtt tgattgatgt
    46861 ttcttttatc gcagctaaag caccgtgagt catttcatca ctatggctgt ataatacatc
    46921 gatttcggat aaatcatttg tttctaaata tcgtttcatc tggtcttttc ctttcatccg
    46981 aataaaatca ccgacaattc ggtgaacaat agtcatcgga ggtttatggt cgatggttcc
    47041 atcttttttt agggttggtt tacgattgat tgattccaaa aatccttcgg agcgtagctt
    47101 ggtcggtgtg gagttctctg aaccagcaag ttctaggatt ctgatttcct gtttctttga
    47161 atttgtgaag tgattgctga caaataaacc cgcccgattt ccttctgctt tgaaactggg
    47221 gccaatgtgg gtgacgaaaa gctcgggatc attcgtttga atatgtctgt cgacaagtat
    47281 gacagggatc cctgctcgct ttgcttcttt taatacagag tcccacccgg attcttgcaa
    47341 aggagtaaaa atgatcatat ccactttata ggcaatgaac gtccgtatat cttggatttg
    47401 ccgttcctga ttcatgtagc catttcggta gagaacttgg taatcttctt tttcaagcgc
    47461 agtgataatt gattcagtat gctttttccg ccagctactc tccgttcctg attgcgaaaa
    47521 accaacgact agtgggtcag gtgcttcctt tgtattaggt gtacacccca tcatttggta
    47581 agcgattaca acaagggcta aaaaaaataa aacacgtttc actttaaatc actccatctg
    47641 gatttgttag tactatctta tcagataaaa gggaaaatca aagtcaaaag gaatcaatcg
    47701 aaaaaaatcc aagaaactta aaaaaatcca agacaaaaaa agtacaccta aaaaatttaa
    47761 agtaaatccc ataaaaaaat caatgttaac gctttcttta ttagctatac tcggtgtatc
    47821 aattgaaatg ggggataaaa aatgaaaaac aaatttagaa agattgttgg gactatcgct
    47881 attttgaccg caagcattct attaagtgcg tgtggcaatt caggatctgc agatgatagt
    47941 acggggtatg taggaatcgc tatgccgacc aaatcagcag aaagatggat cgctgatggg
    48001 aacaacatgg tctcggaatt agaaaaactg ggttataaaa cagatcttca atatggagaa
    48061 gataaagtag aaaatcaagt agcacaaata gaaaatatga ttacaaaagg ggtagataca
    48121 cttgtgattg cttctatcga tggttcggct ttaactgatg tgttagctaa agcaaaagaa
    48181 gcagatatca aggtgatcgc ctatgatcgt ttactgatga attcagaaaa tgtcgattat
    48241 tacgcgactt ttgataattt tggagtaggt gtatcgcaag cagcttatat tgaagagcac
    48301 ttaggattga aagaaggaaa aggaccattc acgattgaat tatttggtgg ctcgcctgac
    48361 gacaacaacg cactaatcaa ttacaacgga gtgatgtctg ttttacaacc atatatggac
    48421 aacggtcagt tggtcgtgcc atctggacag acgagtttca gtcaaatcgc tacgcttcgc
    48481 tgggatggtt ctacggcgca agcaaggatg gataatctgt taagcgcaaa ttatactgac
    48541 caaacattgg atgctgtttt gtcgccatat gatccaataa gtttaggaat catttcgtca
    48601 ttaaaaggcg tggggtatgg gagtgaaagt aaacctttac ctgtgattac tggacaagat
    48661 gcgacagttg ctggcgttaa atcgatcatt gccggggaac aaacacaaac gatctttaaa
    48721 gacacacgta tcttagcaaa aaatacgatt gaaatgatca aagcaatcag tgatggagaa
    48781 gaggttccgg tcaatgatac tgaaacttat gataatgggg tgaaaactgt ccctacgtat
    48841 ttagcaaata cagtatctgt cgataaagat aattatcaag cagagctgat cgacaccgat
    48901 tattataaag aatccgatct gaaaaattaa gaggaaccaa acaaggaact aaagagatct
    48961 aagtcagaga gaggataaga attatggcgg attgtatttt aaaaatggca gatatcacca
    49021 aaaaattttc tggaatccga gcgttagatt gcgtgaatct gatgattgaa cgtggggaaa
    49081 tccatgcatt gtgcggagaa aacggagcgg gaaaatcaac attgatgaat gtcttgagcg
    49141 gcttgtatcc atatggctcc tatgacgggg aaatctttta tgatggaaag ctgtgtcaat
    49201 ttaaagattt gaaagatagt gaagaaaaag gcatcgtcat cattcatcaa gaattagcac
    49261 taagtcctta tctttcaata aaagagaata tttttctcgg aaatgaacaa gcagaaagag
    49321 gcgtgattga ttgggaactg acagaaaaga aaacatatga actgctgaat aaagtcggtt
    49381 tgtctatcga cccgaatctt ctagtatccc agattggcgt agggcaacag caactagttg
    49441 aaatagctaa agcattatca aaatccgtcc gtctgttgat tttggatgag cctaccgctg
    49501 cattgaatga agaagaaagc gcgaatttgc tgagattgat ccgagagttg aaagagcaag
    49561 gtgtaacctc catcattatt tcacataagc tgaatgaaat cgtggatatc gcagatcgaa
    49621 tcaccatttt acgggatgga cagacgattg agacactaga aaatgaaatg atcagcgaag
    49681 agcatattat tcgcgggatg gtgggcagag atttagttga tcgttatcct gaaagaaaac
    49741 cggatattgg agaagtttat tttaaagtag aagactggac ggtctatcac cctcttgatc
    49801 acgagcgaat cataaacgac cacatcaact tgcatttgaa aaaaggagag attgtaggaa
    49861 tagctggact gatgggagcg ggcagaactg aattcgctat gagtctattt ggacattctt
    49921 atggaagcaa gatatccgga cgtatattaa aggaaggggc agagatatca gttaagaccg
    49981 ttccttcagc cattgcaaac ggactcgctt atgtctcgga ggaccgaaaa tctttaggac
    50041 tgaatctgct gatggatatt cgggaaaacg tgacgctgag cagtttggat aaaataagtc
    50101 aaaaaggagt attagataaa gaaaaagaag taactgaggc agaaaatttc cggaaaaaga
    50161 tgcgtataaa aacaaactcc atccatcaaa ttgtcagtag tcttagtggt gggaatcaac
    50221 aaaaagtcgt tttatccaag tggctgatga cacaacccga tattcttttt ttggatgagc
    50281 caacccgtgg gatagatgtt ggggcaaaat atgagatcta cacgattatc gaagaaatgg
    50341 cagcaaatgg caaagctgtc tgtcttatct cttctgaact tcctgaaata ttagggatgt
    50401 gtgatcgaat ctatacgatg aacgaaggac gtattacagg agaagtctcg cgagaagatg
    50461 cagatcagga agtcttgatg aaattaatga cgaaagaaga ggtgggttaa gttggaacag
    50521 gcaaaaaatc ttacggataa aaaagagaaa aaactaaatt tgaaaagtag agcagctgat
    50581 ctttttggtc gctacagcat ggtgatcatt ttagcgggac tacttattgc gtttcaattg
    50641 atgacggatg gtattttctg gcgtccattg aatataacta accttgttct gcaaaatagc
    50701 catatattag tactagctgc agggatgtta ttagttgtgc tcttaggtca tgtggatttg
    50761 tcggtagggt cagtgatggc gtttgttggt gcaattgcgg gcatgatgat ggtcaatcgc
    50821 caaatgaacc cgtggatgac agttatcgca tgtcttgcag taggcgggct gatcggcgca
    50881 tggcaaggtt tttgggtagc ttatgcaggg ataccggcgt ttatcgtgac tttagcagga
    50941 ttgctgatgt ttcgtggact gacacaagtt gttctaggag gccaatctct agcacctttt
    51001 ccagctgttt tccaaaaaat gtctacgggt tatttaccag acttaagcga aggaagcagc
    51061 cttcacttct tgacattgat tttaggaatg atcatcacga tcgtgttagt tgttggtcaa
    51121 tggcgaacaa gaaatcgtcg gaaaaatcat ttatttgaag tggaatctcc aacagctttc
    51181 ttggtaaagg cggtatttac agcagtcgtt gtactaggaa taacttatat ctttgcctct
    51241 tatcaagggt atccgattat tttgatcatt cttggtttga tcgttgcagc atatgggttt
    51301 ttgacaaata aaacagtagc aggtcgacag atttatgcga cgggcggaaa tcgaaaagcg
    51361 gctgaactat cagggatcaa aacaaagcga atcacgttct gggtattcgt caacatggga
    51421 gtcatggcag ctttagctgg attagtttta gctgctcgat tgaatgcggc aacacctcaa
    51481 gcaggtacat cgatggaact ggatgcaatg gcggcagtat attttggtgg tgcttcgact
    51541 tccggcggca ttggaacaat catgggtgcg attgtcggtg gcttggtcat gggtgtattg
    51601 aacaatggca tgtcgatttt aggcgtcggg gtagactggc agcaggcaat caaaggattg
    51661 atcttgcttt tagccgttgt gctagacttg tataataaga aaaaacgaac aacgtaggag
    51721 aacgccggtt attcagaaaa aagactgtga caagacattt gtcacagtct ttttttcgct
    51781 aaaagcgaac attcattgtt tcgtttgtat agactaaaaa taatatatgt ttttttacca
    51841 ttcgtataaa ttctcttttt gctcttgatt tttttgctta attgagttaa attacatagg
    51901 gcgctaaaaa aagccttgat taaattaata atgttcgtgt tttgctggat gtgaacatca
    51961 ggaggataat gtaatgaaag tatttgatta cgaagatatt caattaattc ctaataaatg
    52021 tatcgtaaat agccgttctg aatgtgatac aacggtcaca ttaggtaaac atacattcaa
    52081 aatgccagta gtgcctgcca acatgcagac gattattgat gaaacaatcg cagaattctt
    52141 agcggaaaat ggttatttct atatcatgca tcggtttgat gaagcagctc gaattccgtt
    52201 tatcaaaaaa atgaaaaagc gtggattgat tacctcgatc agtgttggcg tcaagaaaga
    52261 ggaatattcc tttatagaga agttggcaga agaatcgcta aatcctgatt atatcactat
    52321 cgacatcgcg catggacatg ctaactcagt tattgatatg attcagcata ttaaaaaata
    52381 tttgcctgaa acttttgtga ttgccggtaa tgttggtaca ccggaagccg tccgtgaatt
    52441 ggaaaatgcg ggcgcagatg caacaaaagt cgggattggt ccaggaaaag tatgtattac
    52501 aaaaatcaag acaggctttg ggactggagg ctggcaactg gcagctctta gatggtgtgc
    52561 gaaagcagct cgcaagccga tcatcgctga cggtgggatc agaacacatg gcgatattgc
    52621 aaaatctgtc cgttttggtg cgacaatggt catgattggt tcattgtttg ccggtcacga
    52681 ggaatctcct ggcgaaacaa aagtcgaaaa tggaatcgtg tataaagaat actttggttc
    52741 tgcttcagaa ttccaaaaag gcgaaaaacg aaatgttgaa ggaaagaaaa tctggatcca
    52801 acacaaagga tctttgaaag atacactagt ggaaatgcaa caagaccttc aatcatctat
    52861 ttcttatgct ggaggaagag atctggaagc cattcgaaaa gttgactatg tcatcgtgaa
    52921 aaattcgatt tttaatggag acacaatcta aaaaataaat aaaatattag gatgttgtga
    52981 aaaagttgtc ctgcattaag taataagaac agccaaacaa aaatagttct ttatatgttt
    53041 tgtttggctg gaattattat cgcagataca agcttttgaa catcattatt cagaataagg
    53101 gactgtgata agatttttgt cacagtccct tatgttttgc aaaaaaagtt tttctttttt
    53161 ctcgtaaatt cggtaaaatc aaaagaaaaa atagacaata gaaaagagaa gcttatgtgg
    53221 gaaaaagtag agaaaaaaca aggaccgatt tatcgtcaaa ttatggaaca gattatgaga
    53281 gaaatcgaaa acggaaggat caatcctgga agtcaacttg aatcagaacg aaaactaggg
    53341 caactttttg gtgtgaatcg ttccaccgtt gtccatgcat tggaagagct tagggagctt
    53401 ggggtactaa cgagcaaacg tggcagcgga tggtttgtta atcagactga atgggggaaa
    53461 ttcgctgttc caagaatcga ttggcggcaa atgatctctc ctcgttatga gcagacggac
    53521 tggtatgagc aaaaaataag agaagcaaag cagatccaaa aaacatcgtt tcttgattta
    53581 tacgctagcg agatgccaga tgagcttttg ccgaattttg agtttccttc ttattctctt
    53641 gaagaaatct tagcggaaga aaaagaaatg gatcttttag gctatactcc gttacgtaac
    53701 aaaatcaaac ggtatttgga aagtaaattt gaattggcat tttcccccaa ccagcttctt
    53761 gtgactggtg gcggtcagca agcaatcttt ttgattttac aaacgattct ttcagctgga
    53821 gaggggatag ctgtagagtc accttctttt ttttaccgat tacctctttt caaagcttca
    53881 ggaacgcgtt tatttgggat accgatggat gaagaaggga tcgacttaga aaaactgaaa
    53941 gaatcgatcc ataaaaataa attgaaagca gtgcttatca acccgaattt tcaaaaccct
    54001 acaggaaaag tgatgagtca aaaacgacga gaaaagctag tagaattgtg tcgacagtat
    54061 cggcttccaa ttattgaaga cgatgtattt acggatcttt catttctaaa ccaagaagaa
    54121 gtccttccta tacgaacgat tgatccggat aatgtgttat acgtcggttc gttatctagg
    54181 gttttaggaa aaacaacgaa aatcggttgg gtgataggac ctgagacttt tatctcgcaa
    54241 ctagctcaag cgcaagaaat gatggagttt tcgatgaata tccttacaca aatcacagtg
    54301 gctaatgtat ttaatgaagc aattgatttg aaaatgcaag atgtaagaaa aaagttgaaa
    54361 gaaaaaagcc gactgataag cgcttgggga gattcacaat cgtttttcaa gcttcattca
    54421 attttaggtg gttactatgc ctggattact tgggatggga aaaaactaga ccccgaacta
    54481 gcagaaaaag tagtagatat gggtcttgga attgcaccaa gtctactttt tgggcaagat
    54541 ataaatggaa tcagaatcaa ttacagtagg ctggatgaaa accaaatacc gttgttttat
    54601 tcaaagatga aaattctaga aaagtggtta acagaataat ttcacaaagt aaatagatta
    54661 aagaatataa cagcttggat ttaaatttca agctgtttct tttttttcgg aaaaagaata
    54721 cgatataaag aaactaagaa aaaaccatta tttgtacgtc cgttcacgat gtgtaaataa
    54781 aataaatgtt tgcaacaaaa ctgtataaca ccaacgtttt taaatagaaa cagatggttg
    54841 taagtttgat tagaaaatag aaaaaatgca gatgaaatat aaaacgcttt ccttattgtg
    54901 attttattca ctaatgctgt taatatgcgg attggcataa tttcctttca tcgtgaaaat
    54961 ttgtgctttt agcttgtatt tctcataaat ccatgataat ttattaatga agacggttaa
    55021 atagtgatac agttttattc actgtttcaa tcgcagaatg aagagatttt cttatagatg
    55081 agagaggaat gtgtgacaaa tggcaaacaa aaaagcaccg atcgacttcc aagcactgct
    55141 agatgaagtc aatgcagatt tcccagtata tcaagcactt gatcaagatg gaaacgtagt
    55201 taacaaagat ttagtacctg atttatccga tgatgaactc gttgaattaa tgactcatat
    55261 ggtatggtct cgtgtattag accaacgttc aacagcgctg aaccgccaag gacgcctagg
    55321 cttttttgca ccaactgctg gacaagaggc aagtcagctg gctagtgctt acgcatttga
    55381 taaagaagat gttcttcttc caggttatcg ggatgtacct caactagtaa aacatggttt
    55441 acctttatct caagcttttt tatggtctcg aggacatgct gcaggtaatt cttatccgga
    55501 agaattgaaa gctctaccac cacaaattat catcggtgca caatatgtac aagcagccgg
    55561 tgttgcatta ggtttgaaga aacgaaacaa gaaaaatgtc gtcttcactt atactggtga
    55621 tggcggttca tcacaaggtg atttttacga agcaatcaat ttcgctggcg cttaccatgc
    55681 aaatgccgta ttttatatcc aaaacaacgg gtatgcgatc tctacacctc gtgaaaaaca
    55741 aactgcagca aaaacacttg cacaaaaagc tgtcgcagct gggattcctg gtattcaagt
    55801 cgatggaatg gatccgcttg cagtatatac tgtatctaaa atggcacggg aatgggcgat
    55861 cagtggaaat ggaccagtcc taatcgaaac actgacttat cgttatggac cacatacact
    55921 atctggtgac gatccaaccc gctaccgtac aaaagacaca gatgacgatt ggcagaaaaa
    55981 agatccattg atccgcttcc gtaaatattt gacggaaaaa ggactttggt ctgaagaaaa
    56041 agaagaacaa gtcatcgaag caactaaaga agaaatcaaa gcagctattg ctgaagccga
    56101 caaagtacca aaacaaaagg tttcagattt cttgaaaaac atgttcgaag tatctccaca
    56161 atcaatcaaa gaacaaattg caatttatga agcgaaggag tcgaaataat catggcacaa
    56221 aaaacaatga tccaagcaat cacagatgcc ttagcacttg aactagaaaa tgacgagaat
    56281 gtcgttgttt ttggtgaaga tgttgggaaa aacggaggag ttttccgggc aactgaagga
    56341 ttgcaggaaa agttcggaga agaccgagta ttcgatactc ctttagctga atctggaatc
    56401 gcaggattga gtttcggtct tgcgttagaa ggatttcgtc cagttcctga aatccaattc
    56461 tttggtttta ttttcgaagc aatggacgaa gtagtagctc aaatggctcg tacaagatat
    56521 cgaatgagcg gcactagaaa tctacccatc acaattcgtt cgccatttgg tggcggtgtt
    56581 catacaccag aattgcattc ggataattta gaaggtttga tcgctcagtc tcctggtatt
    56641 cgagtagtga ttccttccaa cccttatgat gcaaaaggac tattgatttc ttctattaga
    56701 agtaacgatc cggttgtttt cctagaacac atgaaactat atcgctcatt ccgagaagaa
    56761 gtgccggatg aagcttacga agttcctttg gataaagcag cagttactcg tgagggaaca
    56821 gatgtatcta ttatcacata cggagcaatg gtgcgtgaag caatcaaagc agcagacaat
    56881 cttgcaaaag aaaatatatc tgttgaaatc attgatcttc gtacagttgc gccattagat
    56941 gtagaaacga tcattcaatc cgtggaaaaa actggtcgcg tagttgttgt ccaagaagca
    57001 caacgacaag cgggcgttgc cgcgcaagta gtttctgaga tctcagaacg cgctatcctt
    57061 tcattagaag ccccaatcgg acgagtttct gcacctgata cagtcttccc atttggacaa
    57121 gcagaaaatg tctggttgcc aaatgcaaaa gatatcgaag acaaagtaaa agaaatcgcg
    57181 gaattttaag aaaagaaggg acgaaacaaa gtcatggctt atcaatttaa attgccggat
    57241 atcggtgaag gaatcgcaga aggtgaaatc gtcaaatggt tcgtcaaacc aggagatacg
    57301 atcaatgaag atgatacatt actagaagtt caaaatgaca aatcagtaga agaaatcccg
    57361 tcacctgtta caggaacggt taaaaatgtg attgttccag aaggtaccgt agcaaatgtt
    57421 ggggatgtat tagtagaaat cgatgcacca ggacacgaag acaatgaagg ggacagtggt
    57481 gtagccgcag aatcccaaac tccagctaaa ccggctgctg aacccactgt tgatacagaa
    57541 tcagctggat cgtcaagcga aggagtcttc caatttaagt taccggatat cggtgaagga
    57601 atcgcagaag gtgaaatcgt caaatggttc gtcaaaccag gagatacgat caatgaagat
    57661 gatacattac tagaagttca aaatgacaaa tcagtagaag aaattccgtc acctgtcaca
    57721 gggacagtta aaaacgtgat tgttccagaa ggcactgtag caaatgttgg ggatgtatta
    57781 gtagaaatcg atgctcctgg acacaatagt gcatcgtcaa ctagtgcgcc atcagctgaa
    57841 gcaccaaaag aaaaagtgga aacatcaggt tcagcaagtg tcgttgaagc tgccgatcca
    57901 aataaacgtg tattggctat gccatctgtt cgtcaatttg cccgtgaaaa agatgtagat
    57961 atctcacaag taactgcaac tggaaaaggt ggcagagtaa caaaagagga catcgaaaac
    58021 ttcttggcag gtggtccaag cagtgcacca gcgaaatcag aagcgccaga agcggcagca
    58081 ccgaaagaag cagctcctgc agcagaaagc aaaccagctg caccagcaaa accattcaag
    58141 tccaacttag gagacttgga agaacgtgta gcaatgacgc caacacgtaa agctatcgct
    58201 aaagcaatgg tcaacagtaa gcatacagct ccacacgtga cattgcatga tgaagtagaa
    58261 gtgtctaaac tatgggataa tcggaaacga tttaaagaag ttgctgctgc aaatggtacg
    58321 aaacttactt tcttgccata tgtcgtgaaa gctttaacag cgactgttaa gaaataccca
    58381 gtattgaatg catctatcga cgatgcaaac caagaaatcg tttataaaca ttactacaat
    58441 atcggtattg caactgatac ggatcacgga ctatatgtac caaatgtgaa agatgcagac
    58501 cgcaaaggaa tgtttgctat tgcagacgaa atcaatgaga aagcaaaatt agctcatgac
    58561 ggcaaactat ctgcagaaga tatgcgcaat ggaacaatta cgatcagcaa tatcggatct
    58621 gtaggtggcg gttggtttac accagtcatc aactatccag aagtagctat tttaggtgta
    58681 ggaacgattg cacaacaacc aatcgtaaat gctgaaggcg aaatcgttgt cggtcgtgtg
    58741 atgaaactgt cattaagttt cgatcaccgt atcgtagatg gagcaactgc gcaacaagca
    58801 atgaataata tcaaacgtct tttagctgat ccagagctat taatgatgga aggatgataa
    58861 acaaatggta gttggagatt ttgctgtaga attagataca gttgtaattg gtgcgggacc
    58921 tggtggctat gtagctgcca tccgcgctgc tgaaatggga caaaaagtag caatcattga
    58981 aagagagtat attggcggtg tttgtttgaa tgtcggctgt attccttcaa aagcactgat
    59041 agctgcagga caccattatc aagagtcact agattctgaa atgtttggag taactagtga
    59101 aaacgtcaaa ctagattttg caaaaacaca agaatggaaa gaaaacaaag ttgtccatac
    59161 gttgacttct ggtgttggtt tcctattaaa aaaacataag gttgaaacaa tcgaaggaga
    59221 agcgttcttc gtagatgatc acacactacg cgtcattcat ccagattcag ctcaaactta
    59281 ttcgttcaat aacgcaatca tcgcaacagg ttcccgtcca atcgagattc caggatttaa
    59341 attcggagga cgtgtgttag attctactgg cggattagca ttgaaagaag tacctaagaa
    59401 atttgtgatc atcggtggcg gtgtgattgg tgccgaattg ggcggagcgt acgcaaactt
    59461 aggagcagaa gtaacgattc ttgaaggctc accacaaatt ttaccaactt acgaaaaaga
    59521 tctagtaaaa ttagtagaag atgatttcaa gaaaaaaggc gtaacagtcg taacgaatgc
    59581 aatggcgaaa gaagcagtcg ataacggtga cagcgtgaca gtgaaatatg cagtggacgg
    59641 gaaagaagaa tccgttactg ctgattatgt gatggtcact gtaggtcgtc gtccgaatac
    59701 agatgatatg ggcttagaac aagcgggtgt cgaagtagga gaacgaggat tgatcacggt
    59761 ggataaacaa ggacgtacga acgtacctaa catctatgcc atcggtgata tcgttccagg
    59821 ggcggcatta gcacacaaag ccagttatga agcaaaaatc gctgctgaag caatctctgg
    59881 caaaaaagta gctgtagatt ataaagcaat gccggcagta gccttcactg atcctgaatt
    59941 ggcttctgta ggtatgacga tcaaagaagc aaaagatgct ggtatcgaag caaaagcata
    60001 taaattccca ttttcaggga acggtcgggc attatcgctt ggtaaaacag aaggattcat
    60061 ccgtctagtt acgacgatcg aagataacgt gcttattggc gcgcaaatcg gtggagtcgg
    60121 cgcaagtgat atggtctctg aattagcttt agcaatcgaa tcaggaatga atgctgaaga
    60181 tatcgccttg acgattcatc ctcatccatc tcttggcgaa atcgtcatgg atgcttcgga
    60241 attagcatta ggtttaccaa tccatatttg atttgaataa tacaggcgaa acgcaactga
    60301 aaaatcagtt gcgttttgtt ttttagaatt atcaataata ttattatgta aacttaaaaa
    60361 tatgacaact tcttggttta ttaccacttc ctatgctaca ctgaagagga atatgaaata
    60421 ggagagttga agatgcaaga atatcagtat ccacttgatt tagattggac aacagacgaa
    60481 atggtcattg taatgaagat gtgggaagat ctggaacgtg cgaatgaaaa agggataaac
    60541 aatgaagctt ttttgaagac ttatcagcag tttaaaactg ttgtcaaatc cattggtgag
    60601 gaaaaacgat tgggacggga atttgaaaaa gcttccggtt attctttata tcgtacagtg
    60661 cagcaagcaa aaaaaaatcc aaacaaaact ttgagaatga acgggtgaga taaatggaac
    60721 agatgattga tcatatcaag tcctggcttg aagaagctgg tgagaaaatc aaatcttctc
    60781 aacaaacatt aacaatctcg caaaaatcaa atcgaacgga tctagtaaca aatgtcgatc
    60841 gaaacatcca gaattttcta atcgaaaaaa tccattcctt tgatccagag gcgaaaatat
    60901 tggcagaaga agaagggtgt aatcaattat cggatctcga tggaagagtc tttatcatcg
    60961 atcctattga tggcaccttg aattttgtga ttgaagggga aaatttttgt attatgctcg
    61021 ctgtttatga atccggcgtt ggaaagcttg gtttcattta tgacgtgatg agaaatgaac
    61081 ttttttgggg tggaaaagga ataggcgtgt ataaaaatga tgagccattg cctagaccag
    61141 caaataaatc cttatcggaa ggcttacttg gtgtgaatag ttatcttttc ggtcataata
    61201 aatatggaat ccgagaaatt gcggaaagca gcatggggac acgtatgtac ggatgcgctg
    61261 gtcttgaact agtggcaatc ttgaaaggaa accacattgc ttatatttca aaccttagcc
    61321 cttgggatta tgcagcggga aatgtcttat tggaagagtt tggtatggta acaagcggtc
    61381 tttttggtac tcctttgaca tttgaaaaaa gggaatacta tttagcaggg acttcttgtg
    61441 catatgcaga aatcgcagac cttttgaaaa aagaaggctg aaaaaaaggt agaaatcatt
    61501 cacaaaaatg ataaaaacga gcaaaagatt aattttctcg aaaaaatgaa aacattttac
    61561 tatatttcat gaaaaaagtc tgctataatt agcagtcgag taaaatttgc cctgtcataa
    61621 agaaccaata aaaaaggagt tagttacatt gaattataga aacgatatcc gtaacgtcgc
    61681 aattattgcc cacgttgacc acggaaaaac aacattagtt gatgaactat taaaacaatc
    61741 agatacatta gacgcacata cgcaattgca agaacgtgcg atggactcaa atgcgctaga
    61801 aaaagaacgt ggtatcacga ttttagccaa aaacacagct gtagactata aaggtattcg
    61861 cgtaaacatc atggatactc caggacacgc ggacttcggt ggcgaagtag aacggatcat
    61921 gaaaatggtt gacggcgttg tgttggtcgt agatgcgtat gaaggaacaa tgcctcaaac
    61981 acgttttgta ttgaaaaaag cactggaaca acatatcaca ccaatcgttg tcgtaaacaa
    62041 gattgacaaa ccttctgctc gtcctgagca tgtcgtagat gaagttcttg aactattcat
    62101 cgaattagga gcagatgatg atcagttaga ctttcctgtg atttatgcat ctgcactaaa
    62161 tggtacgtca agcttgtcag atgatccagc agatcaagaa ccaaccatgg cacctatttt
    62221 tgatacaatt attgaaaaaa tccctgctcc tgtcgataac agcgacgaac cattacaatt
    62281 ccaagtttca ttacttgact acaacgatta tgtaggtcgt atcggaatcg gacgtgtatt
    62341 tagaggaaca atcaaagtag gagaccaagt agcattaatt aaattagacg gaacagttaa
    62401 gaaattccgt gtaacgaaat tatttggttt ctttggacta aaacgtctag agattcaaga
    62461 agcaaaagct ggagacttga ttgctgtctc aggtatggaa gatatctttg tcggtgaaac
    62521 agttactcct gtggatcatc aagatgcgtt acctatcctt cacattgacg aaccaacatt
    62581 gcaaatgact ttcttagtta ataattcacc atttgccggt cgagaaggta aattcgtaac
    62641 cgctcgtaag attgaagaac gtttgatggc agaacttcaa acagatgtat ccttacgagt
    62701 tgaaccaaca aactctccag atgcatggac agtttctggt cgtggagaat tgcatttgtc
    62761 tatcttgatt gaaaacatgc gccgtgaggg atacgaattg caagtatctc gtccagaagt
    62821 tattgaaaaa gaaatcgacg gtgtaaaatg cgaaccgttt gaacgcgtgc aaatcgatac
    62881 accagaagaa tatatgggta gtgttatcga gtccttgagc ttacgtaaag gtgaaatgca
    62941 agatatggtc catacaggaa acggacaaat ccgtttgaca ttcttaacac ctgcccgcgg
    63001 tttgattggt tattcaactg aattcctttc aatgacacgt ggttacggaa tcatgaacca
    63061 tacttttgac caatatctgc caatgcttcc tggtcaaatt ggcggacgtc accaaggggc
    63121 gcttgtttcg atcgatacag gaaaagcaac gacttattct atcatgagta tcgaagaacg
    63181 cggtacagtc ttcgtagaac caggtacaga agtatatgaa ggaatgatta tcggggaaaa
    63241 cagccgtgat aatgatttga ctgtcaacat cacgaaagca aaacaaatga caaacgtacg
    63301 gtctgctact aaagaccaaa catctgtcat caaaaaacca aaacaattga cgttggaaga
    63361 atctttggaa ttcttgaatg atgatgaata ttgcgaagta acaccagaaa gcattcgctt
    63421 gagaaaacaa attttagaga aaaatgctcg tgaaaaagca tctaaaaaga aaaaataatc
    63481 tgtaaaagga tgaagagaga aaccagcttg ttataaaaaa tagctggttt ctttttttgt
    63541 ccaactcttt tctgtggaga agtcatcttt gtcaatggac ataaattcag aaaatacgcc
    63601 gaaagacgtt taaaagactt ttaaaaaact tatttctaga gagtttttgt ttcataataa
    63661 gataatgttt cacgaaagga gatcaacttg tccttcgaag aaaatcgaag taaaatggca
    63721 agagaaagga gcgggaagat gaacgactta ttaaaacaat actttggtta tgatgagttt
    63781 agacctggtc aaaaagaaat tatccagaaa gtcattgatc aggaaaatgt attaggaatc
    63841 atgcctaccg gaagtgggaa atcaatctgt taccagcttc cagctttact attagatggg
    63901 cttactgtgg ttgtgtcccc tttaatctct ttgatgaagg atcaggtgga cgcggcgaat
    63961 caattaggta tacccgccac ttttatcaat agctcgttag atggttacga aacagctaga
    64021 cgcttccaag aaatcgatag acagcagtac cgtttacttt atatcgctcc tgaacgtttt
    64081 atcatgccag attttatcca agcaatgaag cgatggaacg tccgtatgat cgctattgac
    64141 gaggcacatt gtatctctca gtgggggcat gatttccgcc caagttattt acagatggcg
    64201 aaccagctag atcaactgcc aaatcgtcct gtaattgttg ctttgacagc tacagcgact
    64261 gtgcaagtag ctgctgacat aaaaagactt ttaaaaattc cagaaaacaa ccatattcag
    64321 actggttttg aacgagaaaa tctacgattt caagtaatta aagatcaaaa aaaagaacaa
    64381 tatctaatcg aatatttaaa gatcaacaaa aatcaatctg gtatcattta tgcagcaacg
    64441 agaaaagaag tagaccgtct ctatcatttg cttaaaaaat tcgatttttc cgtgggtaga
    64501 tatcatggtg gtctaaatga gaatgagcga acagaaatgc aagaagcctt tttatacgat
    64561 cgcttgcagc tgattgtagc gacaaatgca tttggtatgg gtataaataa aagcaatgtc
    64621 cgttttgtta tccattatca aattcctggc tctctagaag catactatca ggaagctggt
    64681 cgggcgggaa gagatggtct ttccagtgaa gccatcctgc ttttctctcc tcaagatatc
    64741 caagtccaaa agttttttat ccagcagtcc caaagagaag aagggcaaaa gcaaaaagaa
    64801 tatgaaaaat taaaagcgat gacagaatac gtgtatattg agtcctgtct tcagcaatac
    64861 atcctaaact attttggcga gacaagcagt ccgtgcaacc gttgtggtaa ctgtttagat
    64921 gacagagaaa tagtagaagt aacgacagaa gcacagatgg ttctttcttg cttaaaacgt
    64981 atgggtgaga actatggtaa acaaatgctg atgaaagtct tagcaggatc gaaagaacaa
    65041 aagctgcaag ctttaggttt tggacattta tctacctatg gactgatgaa aaaccaatcg
    65101 caaaaagaaa cgatgcagtt aattgaatat ttgatatcga atggctatct tctgaccatc
    65161 aatggtgagt atcctgtttt gaaagtaaca gaaagaggga tacaagtatt gaaaggacaa
    65221 gaatctgttt atcgaaagga acccaagaaa gtccagcagc tatcggatga agaaaccgat
    65281 acactatttg aagtgctgcg ggaactgcga acggatctag catcagaagc aggcgttccc
    65341 ccgtatgtcg tattttcaga ttccacattg aaagaaatga gccgaatacg tccatcaagc
    65401 agattggaga tgcttcagat aaaaggagtt ggccaaagca agcttgataa atatggcgaa
    65461 gcctttttat ctcggataaa aaatgcggac tctaacgttc tggataagta gaaagaacga
    65521 atacgtaaaa atgaataaaa acaactggtt aaatctgttt tttttgctcc ttatgaaaat
    65581 caaatatgtt ataattaagg aattagaatg ggaggtaagc acagatggat tctttgtcga
    65641 aaaaacttgc gatccaagta ttaaatgatg aagcagaacg gatcaaacga ttaatccgaa
    65701 atcaaaacaa cagtctctgt atctcacaat gcaaagcctt cgaagaagtg gtagatacac
    65761 agatgtacgg cttttcccgg caagtcggtt ttgctgtccg tataggagta atcggaaaga
    65821 acgagggcca gctgatgtta agtgagctag aacgagaatt gaatcagtta tatagtgagg
    65881 tttaccaaga aatttatgat aaaaaagaag ttggcaagga ggaatagact tgcctaagaa
    65941 agtgaaaaaa aggcatctat tagactatag catcttgatt ccctatttaa ttttatgtgt
    66001 attgggattg atcatggtat atagttctac gtcgtatcta ttattagaaa acggacaaaa
    66061 tccgtccgca tcagtcatta accagagtat tttttgggtg cttagcttga ttgctatcgc
    66121 actactttat aaaatgaaaa cagatgtttt gaaaaaccag cgtttgatca tggcagccat
    66181 cgcagttttg acgattttgc tgctgattgt tgtgtttttt ggtaaggaga tcaatggggc
    66241 gaaaggttgg ctgcaaattg caggattctc gatccaacct gccgaatacc taaaaatcat
    66301 ttctatctgg tacctgtctc tgactttatc taaaagacaa aacagtgtac aaaaagattt
    66361 tcttgggact gtgaaacgtc cacttgcaat ggttatcgga cttactgcat tagtcgctat
    66421 tcttcccgat tttggtaatg cggcagttat ctttttgatt attttggttc tgcttttagc
    66481 cagcggagtc aattatgtgt atactttgat tgtaggagta ggaggctttt gtctaagtac
    66541 ttttacgatc tggctgatca atatcacaaa cggaaagatc ttacccggac gtttacagta
    66601 tatctataat cgatttgcta tttaccaaaa tcctttctca gacgaattaa ataaagggca
    66661 tcaactagtc aacggctact atgccatgtt caacggagga ctatttggca ggggattagg
    66721 taacagtatc cagaaaaaag gatttttaca agaagcacaa accgatttta tttatgcaat
    66781 cgtagtggaa gaactgggag tgattatggg gattttgata ttagcgttgc tattctttat
    66841 gattgtgcgc attgtccttg taggtatccg ttcaaaagat ccgttcaatt cgcttttgtg
    66901 cattgggatc ggcgcaatgt ttttgatcca agtatttgtc aatcttggag gaattactgg
    66961 cgttattcct ttgacaggga tcacgttccc atttttaagt caaggaggct ccagtttgct
    67021 gatgctttcg atttgtgtcg gctttgtcct caacatcagt gcggatgaaa aacgcaagag
    67081 cttaggttta taataaaaaa agatgtagga gtggttcgat gaaaaaagtt ttagttgcta
    67141 acaggggtga aatcgctgta cgtgtatttc gtgcttgtac agaattagga attaaaactg
    67201 ttggaatcta tgcagaagaa gatgaatatt ccgtacaccg tttcaaagcg gatgaagcat
    67261 atttagtagg acaaggaaaa aaaccgattg atgcgtatct agatattgaa gggatcattt
    67321 ctatcgcgaa agaatgcgga gctgacgcta ttcatccggg ttacggtctt ttgtcggaaa
    67381 acttgaattt tgcgcaacgg tgcgaagaag aaggcatcat ttttgttgga cccaaactgc
    67441 atcatttaga tatttttgga gataaaatca aagcaaaagc tgcagctatc gaagcgggga
    67501 ttgcttcgat tccaggcaca gatggaccaa tcgcttctat agacgatgcc ttggagtttg
    67561 cgaaacaata cggttatccg attatgatca aggcagctct aggcggcggc ggacgcggca
    67621 tgcgtgtagc tcacgatgaa aagagtgcaa gagagggcta cgaacgtgcg aaaagcgaag
    67681 caaaagcagc ctttggtagt gatgaagtgt atgttgaaaa atacatcgct aatcctaagc
    67741 atatcgaagt acagattctc ggggatacgc atggaaacgt catccatctt tttgaaagag
    67801 attgttctgt tcagcgccgt caccaaaaag tagtagaagt agcgccttgt gtctctatga
    67861 atgaacagca acgtcaaaaa atctgccagg cagctgtcca attgatgaaa tatgtcgggt
    67921 atgtcaacgc aggtacagta gaatttcttg ttgaaggaga tgatttttac tttatcgaag
    67981 tcaaccctcg cgtccaagtg gaacatacga tcacagaaat gattacagat attgatatcg
    68041 taacaacgca actgttgatt gctcaaggtt tggatctgca taaagaaatc ggattgcctc
    68101 agcaagaagg aattaaatta aatggttcag ccattcaatg ccgaatcact acagaagacc
    68161 cgttgaataa ctttttacca gacacaggga aaatcgatac gtatcgttca cctggtggtt
    68221 ttggcgtacg tctggatgtc ggaaatgctt acgcgggcta tgtagtgaca ccgtattttg
    68281 attcattgct agtaaaagta tgtacgcatg gtgctacctt tgaaacagcc attcagaaaa
    68341 tggaacgttg cttgaaagaa tttagaatcc gtggagtgaa aacaaacatt ccatttatgc
    68401 tcaatgtcat cacacatcct gaattccaat caggaaatgc aaaaacaacc ttcattgata
    68461 gtacagcaac attatttgaa tttcctcgac tacgagaccg cggaaacaaa acgatgaaat
    68521 atatcggtga aattactgtg aatggttttc caggtatcga aagtggcgaa aaaccatttt
    68581 atgaagaacc acgtatgcct aaagatctaa tcacaagaac tgattacgta acggctaaga
    68641 atgtattaga tgcgaagggt gccgatgctt tggtcgaatg gatcaaagga caggaaaatt
    68701 tgttgttgac ggatacgaca tttagagacg cccatcaaag tctgctagca actcgtgttc
    68761 ggacaaagga tttcaagcag attgcacgtt tgactggaga aggtcttcct gaactctttt
    68821 caagtgagat gtggggggga gcaacatttg atgtggctta ccgctttttg aatgaagatc
    68881 cttggcaacg gttgagaaaa attcgttctt taatgcctaa tacgttgctg caaatgcttt
    68941 tcagaggttc aaatgccgtt gggtattcta attatcctga caatgtattg gtggaatttg
    69001 tcaaagaagc agcagcgcaa ggaatcgatg tatttcgaat ctttgatagt ttgaactgga
    69061 ctccacaaat ggaaaaaagt atccaagctg tacgggatac tggtaaaatc gcagaggcag
    69121 ctatttgcta tactggcgat atcaacgatc caagtcgggc aaaatacaat gttcagtatt
    69181 ataaagacat ggcaaaggaa ctagaacaac tgggtgcgca tatcattgca atcaaagata
    69241 tggctgggct gcttaaaccg caggcagctt atcgtttgat tagtgaattg aaagaaacga
    69301 cagatctgcc aatccatctg catactcatg ataccagcgg gaatggaatc atcacttatt
    69361 ctgctgcttc aaaagcagga gtagacatcg ttgacgtggc aatgagtgca atgagtggga
    69421 atactagtca gcctagcatg agcagcttgt attatgcact ggtcaatggt ccacggctgc
    69481 cagaaatcac gattgaaaat gcacagaaat taaaccatta ttgggaagat gtgcgcatgt
    69541 attacaaacc gtttgaaaac ggcttgaatg ctcctgaaac agaagtatat atgcatgaaa
    69601 tgccaggagg acagtattcg aatctacagc aacaggcaaa agctgtcgga ttaggtcatc
    69661 gctgggatga aatcaaacaa atgtatcata ctgtcaactt gatgtttggc gatatcgtta
    69721 aagtaactcc atcatctaaa gtagttggag atatggcact atttatggtc caaaatgatc
    69781 ttacagaaga agatatttat gaaaaaggcg aaacactaag tttccctgaa tctgttgtga
    69841 ctttcttcca aggagaacta gggcaacccg ttggcggatt tcctaaaaaa ttgcaaaaga
    69901 ttattcttaa aggcagacct gcattgaatg aacgtccagg tcttttggct gaatcagtcg
    69961 acttcaacga agtaaaaaaa gaattggcag aaaaaattgg ctatgaacca aaacaagacg
    70021 aagtattaag ctatttgatg tatccgcaag tctttttgga ctatcaaaaa gcatataatc
    70081 aatttggcga tgttacatta ctggacacac caacattttt ccaaggtata cgtttgggtg
    70141 aaacaatcaa tgtacaaatc gaacgcggaa aaacgcttat cattcgttta gatgaaatcg
    70201 gagaacccga tatcgaaggg aatcgcgtac tgttcttcaa tttgaatgga caaagacgtg
    70261 aaatcactat caaagataat tcgatcatca gtacggtcca aacccgattg aaagctgaac
    70321 cgacaaaccg agaacaaatt ggagcaacga tgtctggttc tgttttggat gtattagtga
    70381 aaaaaggcga taacgttaaa aaaggtgata cgttgatgat tactgaagcg atgaaaatgg
    70441 aaacggccat cgaagcacgt tttgacggag aagtcgcaca tgtatatgtg tcttcaggag
    70501 acactatttc gtctggtgac cttttgattg aagtgactga gaaataataa gcgaaaggtg
    70561 gggaaacatg aaaagactgg gtttgttttg gctgattctg ctattagtta ttgtggcatt
    70621 ctacatggaa ccagtcttgt ttccgccaaa acttgaagtg aaggaaaact ctgatcaaca
    70681 aatatcaaac caaatgacaa cgtggaaata ccaggagctt aaagcaactg gtttttctac
    70741 ttatatcgga aaaccgatcg atcagctgga aaaggatttt ggccaagcct atgatcgatt
    70801 aactagcgga tttggttttg aaacaagata ttatgaaaat aaagctagtc atctcacatt
    70861 tgaagcgaac atccaagaca gcagagtcac aactgttaaa gtactgcaaa cagggcctgc
    70921 tgatatcgcg ccgttttatt tagggatgac gatgcaagat cttacgaaaa tcactactat
    70981 ctatactaat ttcacttttg agtaccatga tacagaagtt ggaatcgaac taatggaaga
    71041 ggatatgaat tatcgtccgc taattgcttt tgataacgac acttttgcta ttttattttt
    71101 tgatcaaaca acaaacggcc tattttcagt tgtgtattta gataaagata gtcttttgaa
    71161 actattgcca tatcaagtgt ttggtgaagg ccttccacat tatcaagcag ataaatcggc
    71221 tgattgggaa tcaatcaatc gcgttaaaga acgaaaaagt acgactttat taaatatgct
    71281 tagaagagaa gacgaacttc ccatatacaa tcaaacactt gcctttcaaa accacagtca
    71341 gctgctacta catgattttt tgacaaggcc tgaggaaata ttgtcagagg aacgtatggc
    71401 tgattggcaa aaatcgctag gcagtgctca aacgacagtg aaattctcac tgacgaatga
    71461 tgaattggag aagttaatca gtaaacaaaa attagaaaat actagtgggc tatttattca
    71521 tccggcgatt gacgctactt tttcgttttt atattggtac agcgatcctt acaaccatga
    71581 ccgtttcatg gacccgggaa cggattctgt aggcattgct ttttctaaag aaaatatgtt
    71641 agtcttattg caagaagaaa acggagaaag tagtgatagc agtgatcgtt aacgaagagc
    71701 tgtttcaact ggaggatcaa tgcctccaag tcgctgaaat gataaaaaag agtaaaacga
    71761 tggcttctta tcttgaacat aaacagtcaa tggaagaatc tgcaaaagtt tgccagttga
    71821 aagaagcgtt cttgtctaag aaagaagcat ttgaaaatgt agcggcatat ggtgagtatg
    71881 cacctgatta tcgtgaaaaa aaacgagctg ttcgaaaagc aaaaagacag cttgatatgg
    71941 atgagcatgt tgcagctttc cgatatagtg aaacacagct gcaaagtctg ttggatcaaa
    72001 ttgtccaagt acttgccgaa gaagtttcta atgaaatcaa agtagatgca ggtagcccgt
    72061 tttttgaaac gaaaaaacac tttggatgcg gaggtaattg tcatgcaatt agacaatagt
    72121 gaatttgaaa taacaaaacg acgcgggctg atcgtatggg tctacagctt aaaacaatta
    72181 aagaatctga aaaagttcgg cttgttgcat tatgtctcaa gaagaatgaa atatgtcgtg
    72241 ctttacttaa atgaagagac attggaagaa acagaagaac gaatccgtaa attacatttt
    72301 gttcgaaacg tagaacgttc ttaccgaccg gatatcgaaa tgaattttgc cgaaaaaata
    72361 ggaaacaaaa cagtattaaa agacgaaggt ttcgaaattg aagagctaaa tacagaaatc
    72421 cgattagctg aacatcttta aaaatctctt atgagcagtt tttaaataaa aaataacttg
    72481 gataacgatc gatttttgat cattgtccaa gtattttttt ggtatactag taccagtacc
    72541 atttctaagg agtgagtaga tgcggataat atcaggaaat tatggcgggc gccgtttaaa
    72601 aagtttagca ggggcgaaca ccagaccaac tacagataaa gtaaaagaat ccatattcaa
    72661 tatgattgga ccatattttg atggagagac agtattggat ctgtttgcag gaagcggtgg
    72721 tttagctatc gaagcagtat ctagaggctg cagtcatgcg gtatgcgtag acaaaaatta
    72781 ccaagcgcta aaaatcatca aagaaaacat cgaaattacg aaagaaccag agaaatttac
    72841 cacaataaaa cttgatgcgg acaaagcaat cgaacggcta gcagcggaga aacaacaatt
    72901 cgactacttg tttcttgatc cgccatatgc aaaacaaaaa ataatcgatc aaatccataa
    72961 aatggaagaa ctgggacttt tttctaaaac cgcagttatc gtatgcgaaa cggataaaac
    73021 agtcgaattg ccagaaactg tggctggatt ccatcaaata agaaaacaga tttatggcat
    73081 aacggccgta actatctata gaaaagaggg aaatcaatga aacgtgcatt attcccagga
    73141 agttttgatc cttttacaaa aggtcactta gatacggtgg aacgtgcggc taaattattc
    73201 gatgaggtag taattggtgt ttttatcaat acttcaaaga aaagtttgtt tcccccagaa
    73261 gaaagaatga cattgatcac aaaagctgtt tctcatcttc ctaatgtaaa agtcatgcat
    73321 caagaaaatc aattgacggt agaaacagca aaagagattg gtgcagatgc gttgatcaga
    73381 ggcatacgaa gtatcaaaga ctttgaatat gaacgagaga ttgctcagat gaatcatcat
    73441 ctatatgatg aattggaaac tgtcttctta ttagcaaaac cagaatatag tcacgtgagt
    73501 tccagtattt taaaagaagt actgcacttt ggcggagatg tgagcagcta cttaccgcca
    73561 gtgatcaacg aagctttagc tggaaagcgt gaatcaaatg aaagctaaac aatggttgaa
    73621 aacagtactg attatcatat ttggaatgtt gatcattgga tttgtagtag ttcccttgcc
    73681 gtattatgta gaatcaccgg gagccacgat cgatctgaaa gaactaatta cggtcaatga
    73741 taaggaagat aaaggttctg ggtctttctc tcttacctct gttggtatca ggcaagctac
    73801 aggtttctta gctttaaaag ctaaaatgac cccttttgaa gaattgatca gtgaagaaga
    73861 attgactggt ggggcgacaa atgaagaata taatcaaatg caacagtatt atatggattc
    73921 ttcccaaaat gcagcaatcg aacaagcgtt aaaacttgca gacaagcctt ataaaatgga
    73981 attcaaaggc gtatatgtca tgagtgtaga gaaaacatcg aatttctttg gcaagctttc
    74041 agttggagat acagtaacaa aagtagacgg gaaaagcttt caaagtacag aagcatttat
    74101 ggattatgta aaaagccaaa aagttggaca aacaatcgaa atcagttatt tgcataatgg
    74161 tgaagaaaaa acagcttctg gtgatctgat cgagttacca acagataaaa aggcaggaat
    74221 aggtatcacg cttactgacc ataccgagat cgaatcagat acagatgttc gtattaattc
    74281 tggctctatc ggtgggccat cagcgggatt gatgttcact ttagaaatct atgaacaagt
    74341 cactggtaaa aacttgcgac atggtaagca aattgcagga actggaacga tcaacagtga
    74401 aggagaagtt ggccgaatcg gaggaatcga taaaaaagtg gcaagtgcgg ataaagctgg
    74461 cgtagagatt ttctttgcac cagatgatac gatttcagac gatgttaaga aagaatatcc
    74521 tgaaataaaa accaactacg aagaagcaaa agctgccgct aaaaaattga attcctcaat
    74581 gaaaatcgta ccagtaaaaa cagtacaaga agcgcttgac tatctagaga aaatgaaatg
    74641 aaactaaata attaaagagg ggttgtgaca cgagttctgt cacaacccct ctttttcgtc
    74701 aaaaatcttc acgataaaaa taaaaacaaa agaaaaaatt gacgtaatta gatgttgaag
    74761 ggacgatttt gccagagctc actgtttctt caattttaaa tgaaaggagt aatgaatgat
    74821 ttttactggt cattctttga aaaaagaaca atgatagaaa aaatcagcaa gaaaatacta
    74881 gcttcaccca aattgatcat ttggatgtct atgatagttc ttatcttttg tatcgcaagt
    74941 ggttttttat tatcaaattt gctattttat agagaaacag aagagactgc tgcaaaagaa
    75001 atactttctt cgtttagttc cagtggagaa gatccatcag ctgaaggaaa cgatgtagaa
    75061 gaggatgctc gtccaaaggt catgtacacc gatatcaaag gaagtgtgaa ggagcctggt
    75121 atctattcct tttcttcaga agaacgggtc tatgatgtac taaagcgtgc aggtggatta
    75181 ttagaagaag cggatagcga tcgaatcaac ttttctgcga agatagaaga tcagcaagtc
    75241 ctatatatcc ctgctgtagg tgaagagctg ccagaacatc ttgatcagtt ggcatcctcg
    75301 gaaggaaaac agcctacagc agattccgag cctcccaaaa taaatatcaa tacagccact
    75361 ccatcagaac ttcagcagat tcctgggatc ggatcagtca aagcgcaaga aatcattcga
    75421 tttagagaag aaaacggatc cttccagaaa gtggaagacc tgcaggagat ttctggtatc
    75481 ggtgaaaaga cagttgaaaa actaaaaaac ttcgttacaa ttaaatagaa tgattcaaaa
    75541 tcgactggca cgagcaataa atggacgcta atggatacta agaataatcc aattctaaga
    75601 gcttggcagg acgaggaggt agtatttttg gataggaaat agcttgattg ttcgcaaaga
    75661 aacattaaaa aaaccaatgc tgaatgtcta tctccaaaat aaaatatctg ggattcatat
    75721 tatgaatacg gctgtcagcg gcaataacag tcaagctcta cgagaacgct ttgccaaaga
    75781 tgtacttagc tatacagcag ataaagtatt tattctcatt gggacgaatg atctagcaga
    75841 gcataaacaa ctttctaaag aaacgtacca aaaaatctgc agtggatgat agttcagctg
    75901 aaaaaagact atagttctga tcatgctttc tttatcacgc caccagcagt cgatgagaca
    75961 aaacaaaaat atcggaataa caagttgatc aaagaatata tctctgttct cacagaagta
    76021 gcaaaacgcc atgattgtga agtatcgatt tttatacgga atttcttgaa caaatggatt
    76081 cgaagaattt ttataatggg ctaatggatg acggacttca tttcggcagt gaagggtagg
    76141 atcttttgtc agacaggatg attcgttata tttaaagaat attttgttta agcaatgagt
    76201 actttttaat caacttgaaa aaacggagga atataaaaca tgttagaaaa atcttcaaaa
    76261 gcatgcgata cgaataaaga aaaagttact gataagtcac ataatgaacg cattccttgg
    76321 gaccagtact ttatggctca ggcagtactg ttatctttac gtagtacgtg tacacgttta
    76381 gaagtaggag cgacactcgt tcgagaaaaa aggatcattg ctggcggcta taatggagca
    76441 gtcagtggag atgtccactg catagatgaa gggtgttacg tcgtggatgg acactgcttg
    76501 cgaacgatcc atgcagagat gaatgcactc ctacagtgtg caaaacttgg tatcccgacg
    76561 gaaggttcag aaatctatgt cacacatttt ccatgtttgg cctgtacaaa ggctttgttg
    76621 caagctggtg tgaaagggat caattatctc catgactatc gaaatgatcc ttatgcccaa
    76681 gcgttgatcg aacaattagg tgtatctgta caccaagttg ctttagactc acagcatttt
    76741 tcaaaacttc aaaatgaatt aacaggaaaa catgtctagc gttttagaaa ggcatgttcg
    76801 aaatcggttg atttttccgg caattacgtc gatgattatc tgtttgttgt attacactga
    76861 caattggggt tatcgtttgc ttcttgccgg aattttttta tggttttcgc taaaagaagg
    76921 acgttttatc gcgtacttgt gtattttgtg tagcataacg gtatccattt cttataggct
    76981 acaagagtat tcatttttag aggaaaaaac aacatctgcg caaattactg tatttacaga
    77041 tacgattcgt gcaaatggta attttttgac aatggaagga aggttatcag agaataaaca
    77101 aaagctccag ctttcttatc aagtaaacac agaagcagaa ttgaaaacat ggcttaactt
    77161 ccgaggaaag ggaatagaat tgactgctga aggcgagttc atctctccag caaaaaagcg
    77221 gaatctttat aattttgacc aagaagctta cttcaggaac catagaattt ctggacggtt
    77281 ccaaattcaa aagattgaac agatcaccat gcaaaatcac tggcgaaact ggcttcagag
    77341 aaaaagagga tcttttatct cctttgtcca atctgaattt ccgcaaaaaa caagtgtata
    77401 tattaattct ttattatggg gctataaaga tgcaggattc agagaaccag aagaaatttt
    77461 tagggagagt ggtttgctgc atctgttcag tctatcgggt ctacacatcc aattttattt
    77521 agggtggctg tattatttat ttcgacgtat cggatggcca ctacacgtca gtattattcc
    77581 gttgagtcta ctaactatct gttatgtttc tattgcagga agttctattt ctgttttgcg
    77641 tgctagtttg ctgtttttat tgcgattgct agtcaaattg ctgaataagc attattccac
    77701 catggactta tttgcagttg tcttttggct tcttttacta tttgatcctc gatttctttt
    77761 tcatgcgggt ggacagctta gttttttcat gagttttttg tttatcctga tttcttttga
    77821 tggagattgg ttgaaaaaaa cggcctctca cgtcctgttt actatgatca cgatgccgtt
    77881 gattgtatgg catttttatg agtggcctat tttaggtagt ctttttacat tactgattgc
    77941 accggtattc aagtttcttt ttcttcctgg tgtgttattt ctcacactct tcaatcgctg
    78001 gctgccggaa actttacttt tgctgataga agagggatta atcttatttg agcgaataat
    78061 cgatttttct tcagggtcgt ctctcattat aggacgtttg ccccttgtgc tatctgggat
    78121 gtatattttc ggattactga tttgtatgga taggttgaaa gaacaacctg tgaaacactt
    78181 gttttatgtt ggtctttttt cttttatttt tttgactttg ccatttttac gcttttcttc
    78241 aacgatcgct tttatcgatg tcggtcaagg agacagtatc tttttgcaaa gtccttttcg
    78301 aaaagaaacg attttgatcg atactggagg tcagttacac tttgaaagag aaaagtgggc
    78361 gcaaggtatg atacgtcctc ctgctgaaag aaatatcgtt ccatatctaa aaggtcaggg
    78421 gataagtgta atcgataaac tcatcttgac acacgacgat acggatcatg tgggagaatt
    78481 acccacgtta tctcagcatt ttactatcaa aaaaatttat atcggttggg gcgctggacg
    78541 aaatgagcca ttacaaaggc atttatctga agctcaaaaa aatggtactg agatcattga
    78601 aatcaaacat ggggacagga tcagcggtta ttatgatctt tatgtattga ctccttttga
    78661 aaagggagag ggaagaaatg aagattcgat tggtctatgg atggaatata acaataggcg
    78721 ctttttattt ttaggagatt tgaatcaagc aatggaaaaa caattgcttt tgatttatcc
    78781 taatctgaaa gccgatgtag tcaaattagg tcatcacggt agccgtacgt caagtaacac
    78841 agattttctt tcccatattg aagcaaaaca tgggattata tcctgcggcg taaataatcg
    78901 ttatggtcac ccgcacagcg aagtattgga gacactagaa gaaaatcaga taaaaacatg
    78961 gcgaacggat caacaaggaa tgatcaccta tcgctggcat ccagtttttc atcctcatgg
    79021 gctagtggaa acaatgatag actagtaatt ctcatctgct catggtagga taatttttga
    79081 aagtggtgag taatttgagt atacaagcag agttacaaaa aatacgtaaa gaaaaacttg
    79141 catcttgtta cttgatttta ggaacagaaa agtttctcca agatcaagtt cggacagaaa
    79201 ttgtcaaaaa aatccaatta gagggcgaag atgacttaaa ttttttaagt tttgacatgg
    79261 aaaatgcaag tttagatgag gtagtagctg aagcagagac gttgccgttt ttcggagatc
    79321 aacggttagt attcgtagag aatccttatt ttttaacagg tgaaaaagta aacaatggaa
    79381 tcgaacaaaa tactgattta ttgacggctt atttgaaaga accattagaa tcgactgtat
    79441 tagtattctt tgcgccatat gaaaagttgg atgaacggaa aaaagtaacg aaacaattga
    79501 aaaaaacagc aattatcatc aatgtccaac agttgaatga aaaagaagta cgtcaatatc
    79561 tgatgaatac cttagagaat tcctcaatca aaatggaacg aagtgcaatt gatttattct
    79621 tgaggttgac cgacttagat ttatccaaaa tgatgagaga gttacagaaa ttggtccttt
    79681 atggacaaaa tcaacagcaa atcacggtaa gagaagtaga agaattagtt cctaaaacgt
    79741 tagaacataa tatttttgac atgacccaat atattttaaa agggaagacg gaacaagctt
    79801 tgcgtttata cgaagattta gttatgcaag gggaagaaac aatcaaaatt aatgcgattt
    79861 tgctttctca attacgttta tttttacaga caaaatttct tgtaaagatt ggttatcagc
    79921 aggcaaatat tgctgaaaca ttgaaaatcc atccatatcg tgtaaaactt gccatgcagg
    79981 aagtaaaaaa attcgatgaa catttgctgg tacagttgtt tgatcaatta gttgagatgg
    80041 attatcaaat caaaacggga caaatcgaaa aagaattaag ttttcaacta ttcgttttgc
    80101 gcacagggca gttgttaaga aatcgataag aagaaagacg agggcaaagc atgcgggaag
    80161 gtcgtttgtt tttgtccttt ttttgacaaa taaaaaaatc ggccgaagcc gattctttta
    80221 tttagcgatt tttttgctta aacgagattt gtcgcggtta gctttgtttt tgtggattag
    80281 accttttgat tcagccatat caatagcttt aacagcttct ttatataacg catctacgtt
    80341 gtcagcacct gaagctacag catcttcaaa ttttttgatt gcagtacgca tagcgcttgt
    80401 ttgagatgaa tttttcacat tagcgttttc gcttgtacga acacgtttga ttgcagattc
    80461 aatgtttggc attagtttca cctccgataa cttaagttct tacgcgtgtt accgcataat
    80521 tcaacattca ttattatacc taaaggcagc gtgctttgca atatgaacat gacaagtttt
    80581 tttccttaac agtcttttat taggattttt tttcagtaaa aacggatagt acaagctgaa
    80641 aaaaatcaaa aagacgctat tgtgggaggc aatagcgtca aaccaaacta gatttttatt
    80701 tatctattta aacaaagttt gaatgggaat gtttggtact aagttattat ataacaaaga
    80761 gaaccgtttg caaagttttt tttgaaaagg gatacgattc cctgttgttc tttgtttttc
    80821 tattaagaaa ctgcagttat tttataacca gtatcctttg atcagtaaag gctattctgg
    80881 tgtaccgatt attttaatgt attcatcgat attttttatc aacatttttt ttatgaccaa
    80941 ctggttccga cggttccatt tgccacttac ttccagcaca tatttattta ggggcaaacg
    81001 taagatatat tttgccaaat agcgatcttt aagcaggcca ttgatcacac ggtcctcaag
    81061 ctgcaagttg atcccagtca gcatcattgg ctgttcttgt aaaacgtacg tatcaattaa
    81121 aagaccttca aaagtttcca caagcgtctt cctttctttt caaagtaacc tgaaaacagg
    81181 cactaattct tccattttga tattcgttga agtggatgcg atcattcttt cttttgtcca
    81241 ctgtaatcta gtataacaaa aaagagcaga agagccaaaa agaaaaaaga gcatcgaatt
    81301 ctataggaag tctcattggc acggcctaaa ttattacaaa acattgatta aaaaaagccc
    81361 ctgcatcgca ggagcatccc ctatatcgtt ttaataggtc aatcaaaaag cagtatcaaa
    81421 cacattttgt atcaaatcag gaaataatag ataagctaag cccagaatca aaagtaccgc
    81481 tagaagaaac cagccgattg ttcctatgaa atactttaat ttttcgaaca ttgtatcacc
    81541 tcgttgtcag tatatcacgg atcagtaaat tgggggtgtt aagaatctcc taaaatagac
    81601 aaaatttgtt ctattttcct aaagcaaata cttgtaagat acagtaagtg ataccagaag
    81661 caatgaccgg tccggcggcg attcctctta agaagacaac acctaaaatc gtgccgaaaa
    81721 caagtgcaac agtgatttgt ggatcagaag caatcaatcc tacacccttg gaagataaga
    81781 cagccactag cccaccgcaa aaaacagcta cccagccaat aggggattta aaggcttcta
    81841 atagatcacg tagaccgatt tctcctgttg cgatagggat tagaatagcc gctgtgatga
    81901 tcgtcacccc aatattgatt cctttgcctt gaagccaagg gaaaaaggaa cgggaggctg
    81961 gtatgagctt cataatcaaa acaaaagctg ccgctatcat cagactttga tttttagcta
    82021 aaattgctac aagtaaaata attcctaaaa aaagccaact ttccatgaaa tatattcctc
    82081 actttcttcg aaacaatcat aacatgtttt tagaaagtta caaaagtagg aattcatcct
    82141 tcaagaccta ttcataagct gtttttttca taaaataaac aaaatcataa aaaaacactt
    82201 tgcttatttg acagaagaca gccgcgcttt gtatagtgga caagacttac atttttttga
    82261 agaatcagaa agatcaggta aaggtcttag taaacgcaag ccattcagaa taaccaatat
    82321 tgtgcttcct tcgtgtccta caactccaag aggtagattt aaaaattgca ggaagttaga
    82381 agcaatcaac aagagaataa caagaatcga aaaaataata ttttgtttga tgatccgccg
    82441 catttttaaa gatagccgat gactgtagac aaatttattt aagtcgcttt tcattaagac
    82501 gatgtctgcg atatccatag cggcatctgt cccttgcccc atagcgacac ctaaagaagc
    82561 ggctgctaaa gcaggcgcat cattgactcc atcaccaacc atcgcattta cttggtaaag
    82621 ttcattctct tttttgacta gttgtgtttt ttcttccgga gtacagcttg catagtaatg
    82681 ttcaatccct acttgttcag caatggcctt tgctgtgcca gaatggtcgc cggttatcat
    82741 acttgtatgg atgttttggg atttaaagta ggaaattact tgttgggtat ttgcttttgg
    82801 aatatctagc agacctaaaa cagcaattaa ctgctgatct ttagaaagat aaatcacggt
    82861 tttgccttga ttttccaaac gttcgatcat ttccgcaatc tctttgctga ttatcatcgt
    82921 ctcaggatca tatgcgtgct tgccaacttt ccattgcgca cctaaatagg tagtttgtaa
    82981 tccaaaacca gtaatttctt ctattggtaa gtattggatt tcttcaggta ttgtgcaatc
    83041 gaaatattgc aaaatcgcat gagcaagtgg atgcgtcgtt ttttgttcca tagcaacaag
    83101 aagttgttgt gccaaatcct tttcatcaaa gaaaaagctg tcagttacaa ctggttttcc
    83161 tttagtgagc gttcctgttt tatcaaaggc aatcgctttt aattcagaaa gcttttctaa
    83221 atggataccg cctttgatta aaatcccatt ttttgcagaa tttgaaagag cagctagaga
    83281 agctggtgta gcagatgcga caagcgcaca aggagaagcc acaactaata ataccattcc
    83341 tcgataaaag ctttcatccc aactccaacc taggaaaaag tgggggagaa ggatcattag
    83401 aggaacagca agcaaaatca attttacata attattttcg attcgttcga taaatgaagc
    83461 tgtttgagtt ggcgtattct gcgcttcttc tactaattgg ataatcttag caaagacagt
    83521 ttcggaactc gtttttgtta ctcgaatcgt gatgggattt ccttgattca gcgttcctgc
    83581 atacatctca tcgcctaaat atttttctac aggcacagat tcaccgctta atgcagactc
    83641 atcgatcgtt gcctgtgtgc tttctaaaaa gccatctatc ggaacagctg ctcctttagc
    83701 gacaaatacc aagttatcca cctttagttg agaaacatct acttcttgca tttgtccatt
    83761 ttcttttaat aattgggctt tttgtggttg aagattcatc aaagcagtaa tttctttttg
    83821 acttttatta gttgtgtatt cctctaatgc accgcttaaa caaaagatga atgtcaacat
    83881 tgctccttca aagtaattac cgatcaaaca ggcaccaata gcagctaaag ccatcaataa
    83941 atccacattc agtgtccgat cattccacaa ttctttcaag ccttccgatg tttgtttata
    84001 tccaccgatg ataatggcac tgatataaaa aagtggggtg atggtaagag agaatcggtc
    84061 gaagataaaa ccgaagatca taaataaaag gcagccgatt gttgctgcaa aagctttttt
    84121 ctgattagtc atcgttatta gacctccttg tcaaaacaag gatagcataa taaaaatgta
    84181 attgataata ataacagcta aatgagaacg ataatggtta tcatttggtg attaagcttg
    84241 atgttgtttt tattatttat aatgattatt attaaagaaa ataaaaaacc agaacatttc
    84301 tcaaataaga gaagtgttct ggttttttct acattgcaat aaaccaataa ccaatcaaaa
    84361 tgataccaag gataatacga taccagccaa aagcggtaaa gtcgtttttc ttcaaatagc
    84421 ttagtaagaa tttgatagct aagacggaaa ccacaaaaga tacgaatgtt cctacaagaa
    84481 gaacaacagt ttcttccatt ttgaatgaat tcccttcagc taaatatttg acgatcttca
    84541 agccgcttgc gccaaacatg acaggaatcc ctaagaaaaa tgaaaactct gtagcgacaa
    84601 aacgcgaagc accaatcaag atagatccta ggatcgttgc gccagaacgg gatgttccgg
    84661 gaacaagtgc caagacttgg aataacccaa caagaagagc tgcttggtac gtaaagtcat
    84721 tcagagaagt ccaacgaggt gttttgtttt tgttgcgttt ttcgacaata ataaaggcaa
    84781 taccgtaaac gatcaaaaca atggcaactg gtaagaactt gtggaaattt gcttctaacc
    84841 aatcatccaa aggcaaacca atgatgatgg ctggtatgca agcaacgatc actttaaacc
    84901 aaagtgtcca cgtatctttt ttttctaccg ttgatttact tggagaaaat gggtttaact
    84961 tgtgaaaata aagaacgacg acagccaaga tcgcccctaa ttggataacg acattgaaca
    85021 tcgacataaa ttgtgaactg gcatccagtt tgatgaattc atctgctagg atcaaatgac
    85081 cagtactact gatgggcagc cattccgtta ttccttcaat aataccgaga atcactgctt
    85141 tgataatatt tgcaaaaaac ataaattcct tccttcccaa ttaagataca tcttatagta
    85201 taccgttatt ttttgagaaa cccaatctgt tttttaatat taagaaaaac ttgagaaata
    85261 aagcagataa gtacacgaag agaaaaaaga agacaaagcc ctcgttcaga ctttgccttt
    85321 tatgattctc tggtaaaaat taaaatatcc ccattataga aacgagtcgc gactatgtgt
    85381 tttcccgtta tcgatataag acgtatcatt gatgccgtgg atagaaaatc gaccgtttgc
    85441 ataagtgacc tttgttacgc tgccatttaa aagcccagca cgaacaggct gtttaggatc
    85501 gatcagatta agcaaaaagg cgatcgtcaa accatgagat acgactaaaa catttccacc
    85561 accatttttt tcgcagtgat gggcgatatc ttcaaatcct gtccatacac ggtctcgcaa
    85621 tgtttcataa gtttccgccc acccagcggt atctaagttg tatacagcat tagcaatttg
    85681 ttcaaaagaa acttctgtgg taaacatttc atcatatgtt ttaaaattca atacacgagg
    85741 gatcacaccc cacatttcca tgtcatatcc accttcaaga gacccgaaac accattctct
    85801 aatccttttt tcaataaaat aaggaatttc ttttccctta gaatgttctc ctagaataat
    85861 acgggcagtt tcgattgcac gaccactgtc actagaataa gcaaatgaaa agtcgacatt
    85921 ttttaacccg cgacctaaat aatgaatgcc ttcttgtcct tgctttgtga ggggtgtatc
    85981 acaccagcct tgtactcgtt caattgtatt gaacatcgtt tttccgtgtc gtacgatata
    86041 aagtgtagtt tctgtcattt tattctcctc ctaagtcgtt ttaacgattg aaaaaaggat
    86101 ttgtcctttt ttcctgttca atcgtcgttg catcgccatg tcctggatag actgcaaatt
    86161 catcaggtaa tacaaacagc tgggtccgaa tactatgaag taattgttct aaattccctg
    86221 tatacagatc ggttctaccg atacttcctc taaacagggc gtcgccagaa acgacaaaat
    86281 catcaaaaat aaagctgacg cttcctatag aatgccctgg ggtagggacg acgcgaaaag
    86341 acatgtttcc taaagtataa gttttcattt caaattcata ttctgcaggc gagacaataa
    86401 catcagcaat atcatcatgt ctaccgagtc cagaaagatt caaaatcgga tcacctagcc
    86461 atttttgctc tagtgggctg acataaactg ggatttgata atacgcgcgt aaaggttcca
    86521 ctgcaccgat atgatcgtag tgagtatggg taagcaaaat cgctaccggc tgttggtttg
    86581 tttttttgat ttcttgtttg attcgttccg catcttctcc agggtcaatg atcaaaagtg
    86641 cttcttcgtt gtagaccaaa taacagtttt cttcaatcat tcctgttttt aatctttcga
    86701 tatgcatgtg cattcctcct tgatagccaa aacttctttc atccattagt ataacgcatt
    86761 cattgctagg aaacaaaaac aggtacttat ttctctttac ttatctgaat tatcagacta
    86821 tatgaaaagt atttggatat tctaaagaga aggaaagtaa gttttcatag cattttaata
    86881 tgtccttgtt acattagatt catccaaaaa tccaaattct atatcctata tccctgccta
    86941 atcagcaggg tgttttttta tgtttggaaa gagagctctt tttgctaaac taggtttttt
    87001 caggtaatct tagagagaaa gaggtggtgg aaatgatcaa acagtcaaaa atatctgtgc
    87061 ggcacttagt agaatttatt ttgcgacgtg gaagtatcga caatcgtaaa aaaagtaacc
    87121 atactgcatt agaaggagcg agaatccacc gcaagctcca aaaagatgcg ggagaaaact
    87181 atgaaaaaga agtcttttta aaaacaacag ttcaattaga tgaatattac ctgatcgttg
    87241 aaggccgtgc agacggtatt ttcaaaagag acggtattta ttatatagat gaaatcaaaa
    87301 cctccgaacc acgatttgaa gatttagaac cagaacaagt ggaacttttc tttcatcaag
    87361 caagggtgta tgcctatatc tatagtcaag aacatgattt acaagaaata aatctgcaat
    87421 tgacttactt ccagacggca gaagaagtga tcacgagaaa ggttgaacat cagacaagag
    87481 agcagttaga aacttttttt aaaaagctga ctgaagatta taaagaatgg cttgtttttc
    87541 aagaaaactg gcgcactgta cgcaatgcct ctttgatggc tttgaaattc ccccatgatg
    87601 agtataggaa aggtcagcgt gaactagcgg tagctgctta taaaacgatc cgtacgaaac
    87661 agaaattgtt tgtagaagca cctaccggaa caggaaaaac aatctctacg ttattccccg
    87721 ctttgaaagc tgtaggggaa gaagagggag aaaagatctt ttatctaacg gctaaaacaa
    87781 taactagaca agtagctgaa gatgctatga cagcattaaa agatacagga gctgaagtaa
    87841 aaagtgtcac tttgacggcg aaagacaaga tctgcttttt agatgagacg acctgtaatc
    87901 cagatcagtg cccatatgcc aatggttatt acaatcggat taatgaaggt ttatgggatc
    87961 ttttgaatca tgagaatcag atcactcgtg aagtcatcga aacttatgca agaaagcata
    88021 cactttgtcc tttcgaacta tcattagatg tcagtatctg gtgtgacgta atcattgggg
    88081 attacaatta tttatttgat ccaactgttt atttaagacg tttttttgaa gatgaaaaaa
    88141 acgaggacta tctgtttttg attgacgaag cacataattt ggtgaatcgt tcccgagaga
    88201 tgtattcggc agaactatct tatgaaaaaa cgaaacgaag taaggaagct ataccaaagg
    88261 aattcaaaaa acttcatcgc cgtttcaaca aactattaaa agaatttgat tcgatccgag
    88321 aaatagcaaa agaagatcat tgggattatc atcatcaaaa agcaccggct gaatctttag
    88381 taaaggcagg ttatcaatta agcgaaaaaa ttaaagaatg gctggcagaa tttcctgagc
    88441 acccatcaca agaacaattg cttccttatt attttgattt gcttcatttt ttgaaggtca
    88501 gtgaatttta tgatgatcac tacgaaacaa cagtcgaaaa aacgtatcgc gatctgatag
    88561 taaaagagtt ttgtattgat ccaagcttgt ttcttgagca gtcgttggat aaaggaaaaa
    88621 gcagtattct tttttctgct agtttttcac cactctctta ttatcaggaa acactggggg
    88681 gacaaaaaag tctggcttac aaactgccga gtcctttccc agaaggaaac cagcaagttt
    88741 tcatcgcaaa ttacctagag acgacttatc gaaaaaggga acacagtctg gcaaaattag
    88801 tagagacgat tcatgctttt gctgatggaa aggtcgggaa ttatttcgtt ttctttcctt
    88861 cttatcaata tttagatctt gcggtggaag cattcagaca gcaatatccc gacagcaatg
    88921 tgctaatcca agaaacggac atgaatgagg aagaacgcga acgattttta acaaaatttc
    88981 aaacaaatcc cgctgaaaca ttgattggat tttgcgtact tggcgggatt ttttcagaag
    89041 gaatcgattt gagaggggac cgattgatcg gcagtatgat cgtaggtgta ggtttgccgc
    89101 agatgaatca tgagcaggaa ctgatcaaat catattatga tgagaaggaa caccttggat
    89161 ttgcttacgc ttatcagctt cctggaatga acaaagtctt gcaagctggt ggtcgagtga
    89221 ttcgtggcat ggcggatcaa ggaatcgttg tattggcaga tcaacgattt tctagttttt
    89281 cttatcgacg tttattccca caacattggc aaacagcaag agaagtacga agtgttgggg
    89341 aattggaaga aggaatacag cgtttttggt tatcaaaaga agcagtaaat caaaaaaacg
    89401 aggaatgaca gacgtcagcc tcgttttttg attaacgata gttagtgaat tgcaactcga
    89461 ttggtaagtc aagtttacgt aataaagcaa tcacagcttg tagatcatca cggttcttac
    89521 ctgtcactct aatctgatct tcttggattt gtgcttttac tttcagtttt tcatttttga
    89581 tagctgtagt gattttcttc gcgttttcac gatcgattcc gttgaccaaa tcggcagttt
    89641 gtcgagcttt gccacctaga gcgtgttctg attcggaaaa atggatattt ttgattggca
    89701 catttctttt gatcagctta ccaaataata catctttgat ctgttcgatt ttgaaatcat
    89761 catcgctcaa tacaactaga ttgctgttct ctaatttgat ttctactgta gaatccttga
    89821 agtcaaagcg attggacaat tcttttgttg tttgctgaat cgcatttttt acttcttctg
    89881 tattgacttc tgatacaatg tcgaagctag catcttttgc cataggaacc ctcctgatat
    89941 acatataaat atttaccata catctatctt atcataggat tagggcagat tcttacctgt
    90001 ttgctaaaat tcttctcatt tgagttcgaa gtattttatt ttaatagata aactgttatt
    90061 cttacatttg gaataccaaa gtaggggaga ggtgcttgtg agtaaaatta tgactgcgta
    90121 tcataaaaat gaaagattaa agaaaatagt ggttattttg ataaccggat tgaccgctgc
    90181 ggtcgcttta aattattttt tgattccagc caatgtcttt tctgcaggga tgaatgggat
    90241 cgctcaaatc atttcctcgc ttttatatga atggtttcat atcaagatag acacaggaac
    90301 atttattttt cttttgaata tccctgtgtt tgtattaggg tttctcaaag taggaaaacg
    90361 agcaacgatc cttagttttg tgaatgttgt atgtgtatcg gtgatgacga tgctgcttcc
    90421 agtcggacag atcactgata atattttgat gaatgcccta gtaggcggtg ttttattagg
    90481 tcttggcgta ggattatctt taaaaatggg ctttacaact ggtgggatgg atatcatctc
    90541 tcttgtactt tcacaaacaa ctggaaaaac agtcggaaac tacatgctga ttctaaatgg
    90601 tatcattgta gtcatcgcag gcttcttctt taactgggaa agtgccttgt atacgattat
    90661 ttccatttat gcaatgactc aagtaattga tgccatccac acgagtcatc aaaaagtaac
    90721 agcaatgatc gttaccgtca agccagaaga ggtaacagaa gaactagcaa aacggatggt
    90781 acgtggaatg acattactgc cgtctgtcgg tggctattca aaaaaacaag ggaacatgat
    90841 catgatggtc atcacccgct acgaaatgta tgatcttgat cagatcgttc atgaaatcga
    90901 cgacgatgca tttatcaata ttttaccaac tcattcagta tttggccggt ttgcaaatga
    90961 aaacgaacaa aaaatgtatc gaacaactgg cgtattgcca gaaataaaaa atcgttcaca
    91021 aaaaaaatga ggaatcaggt ttattttaga ataaatttaa tctattacat ctaataagaa
    91081 catttttata aatttaaacg agaatcttac ttctaaattc tcatttacaa gcagctaaaa
    91141 aaagagtatg ataaataaaa atcgtgttaa ggagtgtcgt acgatgagcg aagcaaacca
    91201 agaacggtct ggacaagaaa tagaactaat caaagaacgt attcttgctg ccttggaaat
    91261 ggtgattgat cctgaattag gaatcgacat cgtcaattta ggcttgattt atgatataga
    91321 attcaatcct gaaaatggag aaacagtcat taagatgact ctaacaacta tgggctgtcc
    91381 attagcagat attttaacag aatctatcca tgacgcatta aaagaagtgc cagaagtaac
    91441 gaaagcagaa gtcaaactag tctggtatcc agcatggacg acagataaga tgagccgtta
    91501 tgcacggatt gcgttgggga tacgatagaa atcttggctg actagctatg agaagcaaat
    91561 aggaaaaaca aaaaagcctt ttatcctttc tagaagaaaa gagataaaag gtttctttgt
    91621 ttataaaatt ttgtctgcta tattataagt taattaaata aatgtgttct attgataaag
    91681 gacattcaaa tccctttcct tttggaatcc ttctttttta catcagtttg attttcgtat
    91741 agtattcttt ctcatcttca cgataaatcg cagccccatt tttttgttgt gctttaagac
    91801 tgccaagatt ttttttattt acagacaaaa gtgcctcatc aatgccgatt ttttttgcct
    91861 cttgtagtac taatgctaat ccttgtgtgg catatccttt tttacgataa ttaggggaga
    91921 tgccgtaacc aatatggcca gcgccgtttc ttaatgtttc gttcagttta tggcgcaaat
    91981 taaaaattcc tacatactca tgttcatcac tttctaaaat aaagacggtt gcgggaacgt
    92041 aactctcagg aagatcgatt cctaaagacg ttttttcgca tttttttaca taatctaaaa
    92101 attcagcaaa tgtatagcca taagcattgt tgataaatcc actctcattt ggtccaaagc
    92161 tagtttgtag ctgataagct ttctttaaat ctgcttccca tagtttgatc agtttcatcg
    92221 tattcacctc tttaagtaac cttattcatt tgttttctac tatagcacta ttgttgtaaa
    92281 tagccatgcc tatttttatt tattgtgtca tatcgtatta agaatcatcg aacaatacaa
    92341 atatgaatta caataaaaaa attatttgaa gttcgttcat tcgttactta tgatctctag
    92401 aagaaagaaa acgtgaatta cttcagcgtc tagaattcca gaaggatagt aatcaacaga
    92461 agaaataatc aatatccaaa tgaaagaaga tagctgaatc gagaaaaaga ggaaaaaaga
    92521 aatgaagaaa aaaataaaat agttactaat cccagagtcg ttcgtttaac attgaaatcg
    92581 atcatatgga taaaacagat tttttcacca accctcaata gatgatggag ttgctcggat
    92641 gaattatttg tgtagatggc ataagggagg gcgtacagaa atgaaaaaga acaaaaatat
    92701 gttcttgaca aaaaacacat gtaatcctaa actaaaagat gtgatgcggc tatggcggaa
    92761 ttggcagacg cgctagcttc aggcgctagt gagggtgacc tcgtggaagt tcgagtcttc
    92821 ttagccgcat aatttgtttt tttataaaga gaaggtacac ctggagcgat gagaaagaaa
    92881 ataacatatt atctaaaaga atataggaaa aattttaaac aacggaacca gattcaaata
    92941 aatcaaaaaa aatttgtgtg aaggtgggac agtttaaaac ctaatagatt gattcaaata
    93001 gtagatttat agattctgat acaagaaata ctggcaaacg atagttaagg aataaagact
    93061 aaacttaaac tcaaaaagag gtcatgcgaa agaataaatc gcatgacctc ttactatatt
    93121 ttgaatgtgt ttaaacgttc aaataatgaa tcttagtacc aaccgtttgc ttcccagaat
    93181 gctttcgccg cttcccaaga accataacgt gaagtaacat attgttctgc tactctttct
    93241 tggtttgcag cagagtagtc accatttaag taagaagaat ctaattggta acggccgatg
    93301 taacgtccgt tcgttgctgt gtaagaaccg cttgattctt tttgtgcgat ccattctttt
    93361 gcagagcttg tacttgctgg agtagcagca gtttctgtta cagcaggagc aggttgtgtt
    93421 tcagcaacag gcgctgcttg tgtttccgtt tgaaccgttt cttgtacaac aggttgttca
    93481 gcttgaacag gttcagcttg gactggtgct tcttgaacgg ctgcttgagg ttcagcagct
    93541 gcttgtgcag agccttctgt tgggattgtt aattgttgtc ctgagta
//
LOCUS       DAMYXM010000005        73956 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.005, whole genome shotgun sequence.
ACCESSION   DAMYXM010000005 DAMYXM010000000
VERSION     DAMYXM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 73956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.005"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..268)
                     /locus_tag="PNS21_000422"
     CDS             complement(<1..268)
                     /locus_tag="PNS21_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HDI5158161.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANN"
     gene            962..1348
                     /locus_tag="PNS21_000423"
     CDS             962..1348
                     /locus_tag="PNS21_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDI5158162.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1442..1633
                     /locus_tag="PNS21_000424"
     CDS             1442..1633
                     /locus_tag="PNS21_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5158163.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            2147..2479
                     /locus_tag="PNS21_000425"
     CDS             2147..2479
                     /locus_tag="PNS21_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI5158164.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2775..2930
                     /locus_tag="PNS21_000426"
     CDS             2775..2930
                     /locus_tag="PNS21_000426"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDI5158165.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            3206..4546
                     /locus_tag="PNS21_000427"
                     /pseudo
     CDS             3206..4546
                     /locus_tag="PNS21_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4787..6403)
                     /locus_tag="PNS21_000428"
     CDS             complement(4787..6403)
                     /locus_tag="PNS21_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDI5158166.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            6521..6739
                     /locus_tag="PNS21_000429"
     CDS             6521..6739
                     /locus_tag="PNS21_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5158167.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            6934..7395
                     /locus_tag="PNS21_000430"
     CDS             6934..7395
                     /locus_tag="PNS21_000430"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDI5158168.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(7588..7827)
                     /locus_tag="PNS21_000431"
     CDS             complement(7588..7827)
                     /locus_tag="PNS21_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5158169.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(7851..9374)
                     /locus_tag="PNS21_000432"
     CDS             complement(7851..9374)
                     /locus_tag="PNS21_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDI5158170.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(9392..9514)
                     /locus_tag="PNS21_000433"
     CDS             complement(9392..9514)
                     /locus_tag="PNS21_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDI5158171.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(9544..10527)
                     /locus_tag="PNS21_000434"
     CDS             complement(9544..10527)
                     /locus_tag="PNS21_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDI5158172.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(10551..10700)
                     /gene="rpmG"
                     /locus_tag="PNS21_000435"
     CDS             complement(10551..10700)
                     /gene="rpmG"
                     /locus_tag="PNS21_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDI5158173.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(10721..11098)
                     /locus_tag="PNS21_000436"
     CDS             complement(10721..11098)
                     /locus_tag="PNS21_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158174.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(11130..11309)
                     /gene="rpmF"
                     /locus_tag="PNS21_000437"
     CDS             complement(11130..11309)
                     /gene="rpmF"
                     /locus_tag="PNS21_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDI5158175.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(11324..11593)
                     /gene="rpsN"
                     /locus_tag="PNS21_000438"
     CDS             complement(11324..11593)
                     /gene="rpsN"
                     /locus_tag="PNS21_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDI5158176.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(12116..13882)
                     /locus_tag="PNS21_000439"
     CDS             complement(12116..13882)
                     /locus_tag="PNS21_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5158177.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(13842..15596)
                     /locus_tag="PNS21_000440"
     CDS             complement(13842..15596)
                     /locus_tag="PNS21_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5158178.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(15706..16275)
                     /locus_tag="PNS21_000441"
     CDS             complement(15706..16275)
                     /locus_tag="PNS21_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HDI5158179.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(16293..17717)
                     /locus_tag="PNS21_000442"
                     /pseudo
     CDS             complement(16293..17717)
                     /locus_tag="PNS21_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
     gene            complement(17719..18387)
                     /locus_tag="PNS21_000443"
     CDS             complement(17719..18387)
                     /locus_tag="PNS21_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDI5158180.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(18517..19101)
                     /locus_tag="PNS21_000444"
     CDS             complement(18517..19101)
                     /locus_tag="PNS21_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI5158181.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(19432..19665)
                     /locus_tag="PNS21_000445"
     CDS             complement(19432..19665)
                     /locus_tag="PNS21_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HDI5158182.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(19680..20630)
                     /locus_tag="PNS21_000446"
     CDS             complement(19680..20630)
                     /locus_tag="PNS21_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDI5158183.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(20627..21469)
                     /locus_tag="PNS21_000447"
     CDS             complement(20627..21469)
                     /locus_tag="PNS21_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDI5158184.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(21471..22190)
                     /locus_tag="PNS21_000448"
     CDS             complement(21471..22190)
                     /locus_tag="PNS21_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDI5158185.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            22882..23040
                     /locus_tag="PNS21_000449"
     CDS             22882..23040
                     /locus_tag="PNS21_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5158186.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            23112..24338
                     /locus_tag="PNS21_000450"
     CDS             23112..24338
                     /locus_tag="PNS21_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDI5158187.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(24426..24818)
                     /gene="rpsI"
                     /locus_tag="PNS21_000451"
     CDS             complement(24426..24818)
                     /gene="rpsI"
                     /locus_tag="PNS21_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDI5158188.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(24834..25277)
                     /gene="rplM"
                     /locus_tag="PNS21_000452"
     CDS             complement(24834..25277)
                     /gene="rplM"
                     /locus_tag="PNS21_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDI5158189.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(25796..26263)
                     /locus_tag="PNS21_000453"
     CDS             complement(25796..26263)
                     /locus_tag="PNS21_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDI5158190.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(26383..27921)
                     /locus_tag="PNS21_000454"
     CDS             complement(26383..27921)
                     /locus_tag="PNS21_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDI5158191.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVIGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            28001..28687
                     /locus_tag="PNS21_000455"
     CDS             28001..28687
                     /locus_tag="PNS21_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDI5158192.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(28888..29415)
                     /gene="msrA"
                     /locus_tag="PNS21_000456"
     CDS             complement(28888..29415)
                     /gene="msrA"
                     /locus_tag="PNS21_000456"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDI5158193.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(29499..30176)
                     /gene="pgmB"
                     /locus_tag="PNS21_000457"
     CDS             complement(29499..30176)
                     /gene="pgmB"
                     /locus_tag="PNS21_000457"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDI5158194.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(30173..31303)
                     /locus_tag="PNS21_000458"
     CDS             complement(30173..31303)
                     /locus_tag="PNS21_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDI5158195.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(31303..33486)
                     /locus_tag="PNS21_000459"
     CDS             complement(31303..33486)
                     /locus_tag="PNS21_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDI5158196.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(33483..34508)
                     /locus_tag="PNS21_000460"
     CDS             complement(33483..34508)
                     /locus_tag="PNS21_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDI5158197.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(34553..35380)
                     /locus_tag="PNS21_000461"
     CDS             complement(34553..35380)
                     /locus_tag="PNS21_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDI5158198.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(35377..36444)
                     /locus_tag="PNS21_000462"
     CDS             complement(35377..36444)
                     /locus_tag="PNS21_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDI5158199.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(36470..37306)
                     /locus_tag="PNS21_000463"
     CDS             complement(36470..37306)
                     /locus_tag="PNS21_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDI5158200.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(37303..38172)
                     /locus_tag="PNS21_000464"
     CDS             complement(37303..38172)
                     /locus_tag="PNS21_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDI5158201.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(38242..39525)
                     /locus_tag="PNS21_000465"
     CDS             complement(38242..39525)
                     /locus_tag="PNS21_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDI5158202.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(39539..41224)
                     /locus_tag="PNS21_000466"
     CDS             complement(39539..41224)
                     /locus_tag="PNS21_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HDI5158203.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            41499..42524
                     /locus_tag="PNS21_000467"
     CDS             41499..42524
                     /locus_tag="PNS21_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI5158204.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(43119..46793)
                     /gene="rpoC"
                     /locus_tag="PNS21_000468"
     CDS             complement(43119..46793)
                     /gene="rpoC"
                     /locus_tag="PNS21_000468"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDI5158205.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(46851..50468)
                     /gene="rpoB"
                     /locus_tag="PNS21_000469"
     CDS             complement(46851..50468)
                     /gene="rpoB"
                     /locus_tag="PNS21_000469"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDI5158206.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            51196..52164
                     /locus_tag="PNS21_000470"
     CDS             51196..52164
                     /locus_tag="PNS21_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI5158207.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            52182..53087
                     /locus_tag="PNS21_000471"
     CDS             52182..53087
                     /locus_tag="PNS21_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5158208.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            53084..53890
                     /locus_tag="PNS21_000472"
     CDS             53084..53890
                     /locus_tag="PNS21_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI5158209.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            54026..55015
                     /locus_tag="PNS21_000473"
     CDS             54026..55015
                     /locus_tag="PNS21_000473"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDI5158210.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(55332..55856)
                     /locus_tag="PNS21_000474"
     CDS             complement(55332..55856)
                     /locus_tag="PNS21_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HDI5158211.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            56143..57954
                     /gene="scm"
                     /locus_tag="PNS21_000475"
     CDS             56143..57954
                     /gene="scm"
                     /locus_tag="PNS21_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDI5158212.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(58109..59095)
                     /locus_tag="PNS21_000476"
     CDS             complement(58109..59095)
                     /locus_tag="PNS21_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDI5158213.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(59118..59684)
                     /locus_tag="PNS21_000477"
     CDS             complement(59118..59684)
                     /locus_tag="PNS21_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDI5158214.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(59698..60114)
                     /locus_tag="PNS21_000478"
     CDS             complement(59698..60114)
                     /locus_tag="PNS21_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDI5158215.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(60571..62505)
                     /locus_tag="PNS21_000479"
     CDS             complement(60571..62505)
                     /locus_tag="PNS21_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI5158216.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            62786..63742
                     /locus_tag="PNS21_000480"
     CDS             62786..63742
                     /locus_tag="PNS21_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDI5158217.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            63832..64935
                     /locus_tag="PNS21_000481"
     CDS             63832..64935
                     /locus_tag="PNS21_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDI5158218.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            64948..65901
                     /gene="menA"
                     /locus_tag="PNS21_000482"
     CDS             64948..65901
                     /gene="menA"
                     /locus_tag="PNS21_000482"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDI5158219.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(66715..68061)
                     /gene="gor"
                     /locus_tag="PNS21_000483"
     CDS             complement(66715..68061)
                     /gene="gor"
                     /locus_tag="PNS21_000483"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDI5158220.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(68195..69448)
                     /locus_tag="PNS21_000484"
     CDS             complement(68195..69448)
                     /locus_tag="PNS21_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDI5158221.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(69454..70377)
                     /locus_tag="PNS21_000485"
     CDS             complement(69454..70377)
                     /locus_tag="PNS21_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDI5158222.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(70598..73090)
                     /locus_tag="PNS21_000486"
     CDS             complement(70598..73090)
                     /locus_tag="PNS21_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDI5158223.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(73097..73564)
                     /locus_tag="PNS21_000487"
     CDS             complement(73097..73564)
                     /locus_tag="PNS21_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDI5158224.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(73700..73773)
                     /locus_tag="PNS21_000488"
     tRNA            complement(73700..73773)
                     /locus_tag="PNS21_000488"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(73737..73739),aa:Arg,seq:acg)
     gene            complement(73864..73937)
                     /locus_tag="PNS21_000489"
     tRNA            complement(73864..73937)
                     /locus_tag="PNS21_000489"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(73901..73903),aa:Pro,seq:tgg)
BASE COUNT        22303 a        15065 c        12683 g        23905 t
ORIGIN      
        1 tattatttgc tctaaatgga ccaaataagt aatatctggt tttaaacatg tatagaagaa
       61 acatctcatt aaaatcacca gtaaagaaat ggaaatcttt agttttttta atattatcag
      121 ttagtaactt tttataatca taaaaaaaat aagtagttct atctgagcga tactctttta
      181 taatactgaa ttcggaatta aaaaccacga taggtaactt taaacaaaca tgaagagact
      241 ttaataagac ttctttatct gaaaacactt ttgtttctct ctttcctttc tacatcatta
      301 aatagtattt tatacgttat ctaaaatact attcctctgt caaaataagc taacttactt
      361 cattataaaa tatccactta tcgctttcat gtatattcca ccgtaaaaag tacattttat
      421 caccgagaac tcatcatttt tcatcatgat gaatactatt tacatatatc tagttcaaag
      481 acaataatta ttatgatccg ttattatcta agcactatac agataatagg aatttttcgt
      541 ttctaaatat tccaatttgg gaatccaata aaaattttcg ttcaaatacg tgtctattcg
      601 aactcttttc tagttgttaa ttatgaaggg ggaagaaata aaaaatacaa tctatacacg
      661 aataagaata ttatccaaaa ggaggtttta gaaaagattt attatacgta aaaagctcaa
      721 gacattttct tagccataag gacatgtttg atatttaaga agatatatga attttctttt
      781 ctaagtataa agataactat ccattatcaa ctaaaaaaac atttttgaaa ataaacatca
      841 gcagtcctat caatgcatgt gagcttatag aagttattcg cagcgcagca taattgcatt
      901 agcttctttt cgtgtttttt gcctcttttc cctttaaatc ttaatggagg aggtgtgcaa
      961 tatgtcagat aacgacgaac gcgtaaatgc ccagttgatt cgattctttg aaaaggtcat
     1021 tttgaacacc gctaatactt attataaaaa acaagaagaa attcaaaaac atgaacaact
     1081 tgatcaactc ccaccatatt tgtttactaa agatactata aaatttaaac aaccgattaa
     1141 tgttttaaat gtgactctcc ttgttgaaga tataaaatta gcaaaaatcc ttcctttgtt
     1201 gaaagaacct gaacaaattt ttcttatcga aaaatttatc tttgataaaa ctgataaaga
     1261 aattggaaag gttctgggga ttacaagaca gggtgcgaca aatttaaaac atcgacttta
     1321 caaaaaaatt caaaaaatgc tggtttaata tctgcataat aactaatttt gtatgcttct
     1381 ttgctcgttc tcctccttcc caaagaaata gattataagt ccatgggaag aaggtagatt
     1441 tatgaattct ttaaaagaag atttcatttt agcaaaggct ggaaatgaag aagctgttga
     1501 ggcaattctc aaaagattta gctctttgat tcataagcaa tcctggagaa ctggaaaata
     1561 tgatcaagat tgttatcaag aatgtatgct tgcaatatat ctagctatct cgaaattcga
     1621 aattaaagaa taactcaact aagggagtct gtgacataag taaaaagata gtttcaaatt
     1681 tcgaacaata aatttgaaac tatcttcttt ataattataa agtatcgatt ttacagcgtt
     1741 ctataatgat tcatagaatc taaaacgaat gattttaatt atcaacgttt tttaattcgt
     1801 ttttagctta aaaataactt ttgtcacagt ctcactccta aaattacttt atttacgtat
     1861 gaagtgggca ggaggtcgaa tccagaactg ggctatggta cttcaacgag gacttctcct
     1921 ctagaattga aaagcatgcg atttaccttc cgtaacgacg gtagatttat tcaactatgt
     1981 gtccaatcaa aatttgtctg atttcaaaaa tagtcaagag aattttggtt gttaaaaact
     2041 cttatctatc aacttccaca gtttagttga caaaccctga tactaagttg ggctatgata
     2101 cacttttctt gactatcagt tacgttaccg gaaggaatga actctattga gaaaaacata
     2161 tgatcgtaaa tttaaactaa agatttcaca ggatatcctt gagaaaaaag taactaccaa
     2221 gaaaattgct gaagaataca atatctctcg tccaacaatt tcccgatggg tttcagaata
     2281 tcgtcgatac gggaaaaatg cttttgctgg acaaggaaaa agattacctg ataaagcaga
     2341 tttctacatt cttgaacagg aaaataaacg actaaaagag gaagacgagt gttctacgaa
     2401 cacaaagatc gatacggatc tccagggatc actcaaaaac tttacgatga aggaatcgaa
     2461 atcaataaac gtgttgtagc tgttcttatg cgtgaaatga atttatgcac aagaggattt
     2521 catcgacgca aatcttctca tggtagagaa aaaacaatca aagaaatggt aaaaaaatgt
     2581 tgaatcgata atttgaacag aaccaaattg atgctgtttg ggtgatggat attacttaca
     2641 ttccatgtaa tgacggtcgc ctctatttgt ccacctatat tgacttagca actcgtattc
     2701 tacgttacta tatggtagat tcacacatga aaaaagagat tgtcgtccgt ccattacaaa
     2761 tttattaagg agaattgcct acggtaattc attccgatag aggtagccag catcggtctt
     2821 ttatttatca agagctgttg aacgaccacc acatcacaca ttcagtgagc caaactggga
     2881 ctcctgttga caatgcagta attgaagcgt ttcatcgatc aatcaaatga gaattaatcg
     2941 actccaacaa atataaaagt aaaatagaga tgaaggtgtt aatccaagat tatttagaag
     3001 actactaccc aaacaaaaga atccatacaa aatttatgat gactccttga caatttgaag
     3061 cgaagaaaat aagggatcta taatatctag tgttggtgac gccttctgtt gccaatactt
     3121 tttctttttt ctaataaacg caaacagaca ccttaaaatc ctttagaata aagtcgacga
     3181 aaaccattca aaggagtgtt tcaagatgtc ctattcagag tttaccaaag agttactaga
     3241 tattctagac ctaaatctta tctttcatga agatgccttt cgcaaagagc gaatcaatga
     3301 cgaaacatgt tttgtttttg atggcacatt gacctatcaa ccggaggaat gctttcatcg
     3361 ccactatcaa aatgaacaaa cgatcattaa atagggctgg aaaaaagtat ccatcttatt
     3421 gaatgatgtc agtaactaca agaccattct gcggattaac aaacagcgct tcaaatgtaa
     3481 acactgtgga aagacttttt tagcagaaga ttcagttagt gatcgtcgct gctccattgc
     3541 tcgcagagtc aaacaagcca ttcttgagct attaagcgaa cctctttcta tgtctttgat
     3601 cgcacgtatg aagcacattt ctccgaccac tgtcattcga atacttcgca gccttcggcc
     3661 taaaaccatt tctttgagcc agctgttacc agaagtggtg tgtttcgatg aattcaaatc
     3721 agtgaaaaat gtctcgggag ccatgagctt tgtcatgatg gacggcaaga cccatcaact
     3781 gattgatatt gtggaaaatc ggcaactcaa tcctttacgc gactatttct tgcgctaccc
     3841 tataaaagtc cgtgaacaag tgcgtctagt ggtttcagat ttctatacac cctatcgcac
     3901 attggtgaaa gagtgttttc cgaatgcccg aatcgtcgcc gatcgctttc atattagcca
     3961 acacattggt cgggctttta ccaaccaccg aattcaagta atgaagacct ttaaaaaagg
     4021 ggatcgtcgg tcgaagcact taaagaaata ctggaaacta cttcaaaaga atgcgtggga
     4081 attgaaaggt cagcatcgct actggcgtcc gtcatttcga gatcatctga cagaagcaga
     4141 gatagtcgat cggctattat cttatgatga ttccttaaag cgaggctacg aagtctacca
     4201 ggattttctt tccgttatca gaagacaaga tgttcctgaa ttcgtcgcgc tcctaaaaga
     4261 agactacaag gagttgccag agcactacca accagtgttc acgaccttca agaaatacca
     4321 aacagaaatc aaacgagcgt tgcgcgttcc ctattccaac ggccctatag aatgtttgaa
     4381 taaccatatc aaagtactaa aacgaattgc ctatggctat agaaactttc aaaactaccg
     4441 agaacgaatc tttttatatc gaggaaaata ttttaagaaa actaagaata ctacccaact
     4501 gactaaagcc agaacaacaa cacggctaga caagatagct gtctagccgt gtgagtatct
     4561 cgattattct attgaatttc ttcaccaaca ctcattgacg aagagccaaa ataagtaatc
     4621 taaccgactg ttaactaaac cattgcaggt cccgttaaag atacttgtgc ctgttgtcac
     4681 cgcttctttt tcttgtccac aagcgcttaa cacacttaat aaagcccctg ctattattaa
     4741 taataatatc tttttcttat taaaaacctc ctcatctatt tccagattag taaatgtagt
     4801 aatctgtgct ttcttttttc tcaatactaa taactaactt atggggtaaa caaatactcg
     4861 tttgaccggg ttttgagatc catccagttc taaccgcgat ttgatcagga ctattatctt
     4921 ctttaactcg aattcttcca tctttaatct caacaatatt atattgtccc ttcgcaggat
     4981 gataattcac gagtttatga ttgatcttat ccaaatcgaa gcgatccatt tccttgccat
     5041 caattcgaac gatggcatat ttgatagcat cttcagttgt ttcattctcc ttttctatag
     5101 agaatacaaa taagggaata aaagaggtaa taatcaaaat gccaataatt atgacatcgc
     5161 ctatcttgac accagctttt cttaaaaatt tcattaacga tatctggcta aacatattag
     5221 actttttcca tggaaatggt gaaatttttc ggaaattatc tgcattaaga gaatcagtta
     5281 aacaaccctc aagttgttcc ttaatacatt gttgatccat cttcatacca ataaaaacta
     5341 gtttattaat gcgttcacca aacaacggat gccaggcttc cttcaattcg ggattttttt
     5401 gtataatcat ttgacactca attttaggta aactagctac ccaataattt aatgattcaa
     5461 tacttttata gctacccgat gtttcaaaga tcgtcgaaaa atcactatgt tgggcaaacc
     5521 aaagaaaccc ttttgctctt gtaatattgc caggaaaatt tttatccgta agatgggcaa
     5581 atttttgtgc gtcaaggagt aaccgatttc gatagataaa acttgagata ccatattctt
     5641 ctgtttcagg aatatgattt tgatagccaa gctctaactc tttgaaccag cccactgaat
     5701 aggaagtctc atctatatta aaaagatttg tatttagaat tttcgataag tctacttcac
     5761 ctttaattgt cctaattatt ttggcattgg actgtaactt agcaattaga ccgttaattt
     5821 tgtaaatcgt cttggttgac actaagtcac tattatttaa aagtaatatg ttacaacact
     5881 cgaattgatc aattaacaag ccacgaatat cctgttcttc ttcaactaca ttaccttcag
     5941 cttttctttc ctgaatcttt tcacccgttt cgaaattatc ccaaaaacgt tttacatcca
     6001 ctactataat aatggcacca atctctaatc gttgggtcaa atcaatgtct aaagggtttt
     6061 tagctaatat aattgcttga ataattggaa ttggtttgct gattcctgaa gcttcaataa
     6121 caatttgatt caagtcagaa aattgcacta attgatctag ttcaacaatc aaatcttcgc
     6181 gtaatgtaca acaaatgcat ccattcggca tcgaaacaag tttttctcca gttcttttaa
     6241 aacctttctt gtcgactaat accccatcaa catttacttc ccctatgtca tttacaatga
     6301 cagcaatttc ctttccgtga tcaccgtaca gaatatgatt tagaagtatc gtttttcctg
     6361 aaccaagata gccagttaag attgtaatag gtattttttt catagggaac ctctttctat
     6421 accttgaatt tacactttaa gtgcaacaca taaaaatgag tcataatcga tatccaacta
     6481 gtataagttt gtcgagaaca ctcctagaag gaaaataatt atgagccagt atcgtcatct
     6541 tactatactc gaacgagaaa gaatctttct gcttcacgaa gaaggatttt ctattagtat
     6601 aattggcaac gacattcagc atagcccgtt tactatttta catgaactga gaagaaacaa
     6661 gaaaaaaact attcaccttc caaagcggaa aaaaattatg cttatcgaag aaaaaaatgc
     6721 ggacgttatc acattctaaa tgatccgtga gtttggtcaa ttgttcgtca tttatttgtc
     6781 gattttcagt ggtcgccaga ggaaatctcg aatcgtttga agcatgaaca tatggacttt
     6841 tcaattagtt ttatcacgat ctaacgtgca atttgcaatg atcttttcga tacagaaccc
     6901 ctctcacacg gcaataaagg cctgatccgt tcattgcacc atcgaggaaa aacaagacat
     6961 acaaaaaatc atgtcgagtc tcgtggaaag atttgcctta ctcattcgat ccatggccgt
     7021 ccaaaatccg cagatgatag aagtgatttt ggtcattgga aagccgatat tgtagctggc
     7081 aaaactggtg gtacttgttt agtgactttg acagacagaa aatcatgctt ccttttagca
     7141 ggaaagaagg tccccagaaa gaaagccgct tatgtgagcg agaaaatgat tgaactttta
     7201 agcagtattc caaatgaggc ggtcaaaacg atcactccag atagaggaaa agaattctcc
     7261 aatcatttcg cagttacaga agcattgaat aatatgccct tttattttcc agatcctcat
     7321 gcccttggca acaatgaaca aattaaaata cgaatagact cattcgagaa tacttgccaa
     7381 aatcgaaaga gatagctacg gtagataact caacgataaa tcagatgatt gaaaaactga
     7441 ataacagacc tagaaagtgt ttagattgga gaacacctta tgaagtcttc tttgataaag
     7501 tgttgcgctt gatttgacaa ttcaagacat aataaaaagc agatcaaatc caaataaggg
     7561 aaaaatgatc tactctggta ttatcaatta attggccaag ctattatgcc gttttgctaa
     7621 acgtctactt cttccagtat tgaccttgat aaaacaacaa tcaggatccc gttttaatac
     7681 tctatgaact gtgtcaatac taacatgtaa agttgcagca atatcagcta atgtactccc
     7741 atttaatctc atttgctttg caaatcgata acgccgataa gcatcaatat tcttggaatc
     7801 catagacaat aatacttttc ttaccatgta tttcctccaa aaattgaatt ttaatgagcc
     7861 atcatttctt gagcaatttc atgtttagac aatgaagctg gatagaaagt aggccaatta
     7921 tgcaattcat tatagagttt ttcttgactt tctccaccaa agaaaatatg gaaatgctct
     7981 gccttacttg gtgcaattcc atgatcacta aattgaatat atttaaaacg accagcacct
     8041 gcatcatcag tttcaaaatt aaaacgaaca ccacggtttc ctttttcata attcaaaatt
     8101 ttgtatccaa catatttgta tgtgtattga tgatgttcac cgttgactac aaaatcaatg
     8161 gtgttgtctt taatgttgat agtatcaata tctgtcttat agccagtagt ataataatct
     8221 ttgtactcgg cagctgtcat gtctttattg atttttgctt tgtaatcgaa aacttcatcc
     8281 aaaattccat cttttaacaa cggataaaca gattgccatt cgcctgtata atcactcaat
     8341 tcgcgatcct tcacatcggc atctttaaag tacccattag caactgtctt ttcatccttc
     8401 gcttcttttt ctggaactac ctcttcacca gcaaccatag ttgttttttc taacgctttt
     8461 agattgtctt ccattactga aacataatct tcaccattat ccatttgttc ttttgtaaga
     8521 ctttcaagag gattcagtac ttccaatttt atcccagctt cgttggccaa agtttttgca
     8581 attttatcat tagcattctt ttcaaagtaa atataattga tcttattttt tttaacatac
     8641 tcttttaatt cagctaatcg accagaagat ggctcttctt caggacttag tccagcaata
     8701 ggaacttgtt tcaatccata atccaaagct aaatatccaa aggctgcgtg ttgcgtcaca
     8761 aaactttttt gtttcgcttc tttaagacta gtggtatagt cagcatccaa ccgtttcagt
     8821 ttagtcaaat acttttcagc atttgtctca aatacttttg cttttttcgg atacaattta
     8881 actaactgat ccttgatatt tgaaacttcc ttaattgcca ttttgggtga aacccacgta
     8941 tgaggatcta gttcatgatg gtgatcttct tctccgtgat catgatcttc ttcttcacta
     9001 cctggcaata aaatcatgtc ttttgttcct tctactacat taggcccagc tttcttccaa
     9061 ctttctttag cttttggtac ccatgattcc atgttttcgt tatgatatac aaaaacatca
     9121 gcatcatgag cttttgccat atccttagct gagggctcat agtcatgagg ttctgatccc
     9181 gctggaacca ttaaatccac gttcgcttca tcgccgacga tgtttttggt gaaatcatac
     9241 attggataga atgttgttac aatttctaat tttccatctt tatcattcga tacttttgca
     9301 tcattagtgc tggagcaagc tcccaataat cctagagctg ctatcccaat aagtcctaca
     9361 ctgattttct tcattctttt tccttcctta attatgctaa tttcttcatt ttatttcgtt
     9421 tatgttcttt gataattttt aatgcccttt ttctagtttt aatattcggg cttttaagtc
     9481 gtcgatatgc actagatata tccgttttct ccattagaaa ttacccccaa tcactactta
     9541 tcattatttg ctttccgcca gtaatttatc aaaactctgt ttgatccttt cttcatccaa
     9601 catttttccg attagaataa tagtggtttg gtctttttgt cgagtggtgt attgaaaatt
     9661 aacctgttcg tttacctctt gcatggcaac tataaaatcc tgctcttgta aagcaatcaa
     9721 tcctttaaaa cgatacaggc tcccttggtt ggttaaaatc agccagtcaa tccattggag
     9781 taaaaaatct ttttttatgt cttgatctga agttaagaga attgatttaa actcatgtac
     9841 ttgatggtgt tgatgatagt atttgccatt atagtgattt tctgatgaat cactttcaaa
     9901 aactaaggga tgattaaatt ttttcaaatt ataaaaatcc tttttttgaa tcgtttcact
     9961 atgtgtgaac attcgaatat cggataaagg gttaatactt ttgatttctc tctgtaattt
    10021 ttttagtgca gataaattca agttttgttt tctagacaca aaaatgcgat ctgccatgac
    10081 aagttgctta agggcttgtg gttctctttc aataagttca aaactgtcac tatcaataac
    10141 agtcaataaa ctatcgatat atactcttcc tcttagttga ggcgctgtta aaagtgtttg
    10201 aatgattggt tggggatctg ctatacctgt agtttcaatg atgatttgtg aaattggttg
    10261 ttgatgttct tctattgttt ccagtaaaga aatcaatatt gctaataaat cactgcgaag
    10321 actgcaacac atacatccat tatttagttg taacacccgt tcctcgctat gaactaataa
    10381 tttgtgatca atcccaactt caccaaactc attttcaatt ataataattt cttccctggg
    10441 gtagggtgag acttgcaagg cttgattgat tagcgttgtt tttccagccc ctaaaaaccc
    10501 agaaacgatt gtaactggta tgcccatttt gtcacctttt taccacttag ctatttcact
    10561 tctttaaaaa ttgcccgtct gcgcaatttt ggcgaatact tttttaattc taaccgttca
    10621 gggttattcc gtttattttt gctggtgaga tacagacgtt ctccagtttc gacacattcc
    10681 aaaataatat tttgacgcat aaatcttcct ccttcaatac ttatcgatct tcaagatatg
    10741 cttgaatttg ttgtgcatct tcaaaagtta actcgccaga ttccaattgt gaaactaaat
    10801 tttctttcat atcaacaggt atctcccaat aaccatcgat acattgaaat aactcctcgt
    10861 catactcttc aaaaattgga tagattctta aaaaatcatc aaatgataag ccttggtcct
    10921 catagtaatc tttgactaat ccattttcaa ttgcctgttg ggtcggtaaa cccgtttcag
    10981 taataattcc tctctctacc atcgaacgat aaaaagtgca tttatctagc gattgctggg
    11041 tcgtttcaag ataatacttt aatccttctt caactaaagg tgggtaaata atttccattt
    11101 tgattccctc ctaaaagtta tatattaaac tattttgaag ctcctttaat tactttcttt
    11161 ccattatagt acccttttaa agatacatga tggctacgtc tataatcacc attcgcttcg
    11221 tcaaatgaaa tttctggttt tactacagtt tgatgagttc ttcttaaccg tttcttcgcc
    11281 ttagatgttt ttcttgctgg tactgccatg atatttcctc cttttaccaa cttgcttttt
    11341 ttactccagg aatcaaccct tgatgggcaa gttctctaaa cttaatacgg gacataccga
    11401 actttcgcat ataccctctt ggacgaccat ccgtctgatc acgaagcctc agacgattcg
    11461 gatttgaatc tcttggcaat ttagcgagag cggtgcgatc tcctgcggct tttaattcct
    11521 gacgtttatc tgcatacttt tcaatcatcg ccatttgttt ctttgcttta gcaattttcg
    11581 acttttttgc catatacctt tatactcctt ttattttgat ttagacacct caaatcgtaa
    11641 tcgttacgtt ttgacactga tactttacca acaagagttg ctcttgtcaa caataaataa
    11701 catgaaataa aactcatatg aatagataac tatttgaaaa cgaatttatc ggtacataga
    11761 gataatcaaa tcatttaaaa gaatatgcag tttaggctct gaaaaataag aattcacttt
    11821 tattagtcca atcaaacaca aataccgttt taagtagctt ttcctctact taaaacggca
    11881 atatgaaaga aactataact ttttattcaa tcttttatgt ttaactggtt tatcgccaat
    11941 tttttaaaac tttgattatt ccttatatta taagaataga cattaagtga actaattcaa
    12001 tcatactgat tctttcgtgt attcaaaatt agatgctgtt taataaaatt attctttttg
    12061 aaaaagaatt ataactatat cccaaaaatt gtaggttaat ttaaagccta tttcatcatc
    12121 gtgattgagc ttctttttca tagacgaatt tctcagatag tttagttttc tttatgagat
    12181 ttttgtatat ttctgacctt cgatacaact cattatgagt accttgatca actagttttc
    12241 cttgatccat cactacaatt ttatttacct tttcaataga cttcaagcga tgcgatataa
    12301 ttagtactgt cttatcttgt attaaacgat ttaaactttc ctgaatacgt ttttcatttt
    12361 ccgcatctaa actggcagta atttcatcta aaattaaaat tggcgcattt ttcaaaaaag
    12421 cccgtgctat ggataggcgt tgtcgctctc ctccagaaag tgaacttccg ttttctccga
    12481 ccaatgtatg ataaccgtca ggtagttttt caataaagtc ttcgcagtta gctaaacgag
    12541 ctgctttttt cacttcctca tctgacgctt gactatttcc aatacgtata ttttccaaaa
    12601 tacttgtatt aaacaaagtt acctcttgaa aaacaatagc gattttgcta aacaaagaag
    12661 ctggagatac acgtttaata tcatacccat caattaaaat acagccttca tcataatcat
    12721 acagtctaga cactaatttc aagatactag ttttcccgga accactagca cctactaacg
    12781 cagtcacttc tccttgttta gccgtaaaag agatatgatc taaaattggg gtattgttat
    12841 catatgagaa agagacatct cgcaactcaa tgtcaaaaga tttcagagga ctatctgatc
    12901 cctcttggat actggtttcc ttcatagctc taattcgctg tattttagga gctaaataaa
    12961 atatttcaag cactgcttct ttcatagaat cgaaggaatc ctttattttt attgcggcta
    13021 gtaagtaacc aacgacgtag agtatagtca cttcacccgt taacagtaag tgaacaccta
    13081 ctagaatgac tactgctaaa ctaacatatg agaaaataga ggataaggcc atgactgaaa
    13141 aagtacttaa ttctaccttt aaatgaatcc tttcactctc ttccattttt ttgaaaagtc
    13201 tgtcttgaac ttttttagat agattaaagc tattaatctc ttgctgcaat tctatagttt
    13261 cttgaaattc ttccgagttt tctctcaaag tatcgtaata tttagtattg gctttcgttt
    13321 gggatttctt tgataacaag attaagacaa aactaaataa cgttggcaaa ataacagcta
    13381 atcccatttt gacattacca accaaaagca tcactgaaat aaaagggaaa aacagagcca
    13441 taccgccggc tttaggtatt gcatgactca tcgcatgctc aataccttca acgtcagaca
    13501 tgatagtttg agataaatct gataaattat gttttgaaaa ataggataat ggtaaatttg
    13561 acaaatcgtc tgcaatttgc actcttaaat gcgcactttc tttataagta ctattatata
    13621 agttttcata ctccctatct aacaaccaat acatgaaaag caaagtaaca aacgatacgg
    13681 caaaataaag ccaacggggc tttgtatttt ctaaaactaa ttcgtctatc agcatcatta
    13741 taatgaatgc tggtaccata ttaatgcagt atacaaaaaa actagaaata gaagctttag
    13801 ttaaagcttc tgcgccctga tcacttaaag caaatttgtt tttataaaaa cttttcattg
    13861 tcaagcctcc aatcatttgc attctcataa ttagtgacca attgtttata cacttgatgt
    13921 ttagccatta attcattatg atttccacgt tctactattt tcccttctga caagacaaga
    13981 atttcatcta cagatttaat gctggataat cgatgagcta tcataataac tgttttttct
    14041 ttcatcagat gttgaaaagc cttttgtagt tcatactcat tatctggatc aatagatgca
    14101 ctggcttcat ccataataac aatctttgca tcttttaaaa ttgctctggc aatagctata
    14161 cgttgttttt ctcctcctga cagataaact cctttactac caattaaggt atgctctctt
    14221 tcgggaaatt tttctaaaat cgattcgcaa ccggctaatc ttagcgcgtc aaatacttta
    14281 cttgctggcg cttgaggatt tgctatagaa acgttatcgt aaatactctg attgaatagt
    14341 ttcgtatctt gaaagacaaa agcaattgtg ttaataattg tgtcttttga atagtgattt
    14401 aaaggcttac caccaatttt aatctgtccc ttatccacat tgtagaaacc tgaaattagt
    14461 ttagctatcg tactctttcc gcttcctgat tttcctacta atgcataaat tcttttttgt
    14521 tctaaaacta agttgaaatt ttctaaaaca cactcttctt tgtatgaaaa gctgacattt
    14581 tcaaatgcaa tgtcacaatt tttaatctgt gtctcgttcc catattttag ttggtcttgc
    14641 ttcatttcta aataaaggga gtctagtgtt tcgactgcat atcttgcttc aaacaagtgc
    14701 atacttacat acattattct catgaaagaa acaaaaataa ttccacttaa aaaaaacaac
    14761 atcaacaagt caacagctaa tatcttggga gtggtaagag aagttataaa aaaggttata
    14821 ggaactatca aaatgcaaac tatgccaaaa aatagccatt gaaaaagaac aaaaggtgtt
    14881 ttcccactca aagaatactt atatgcaaac ttagcataat cgttaattgc tttattcaag
    14941 gctttaaatg atgttacttt agcaccaaat atctttataa cttgaattcc gcggatatat
    15001 tctaccgttt cagagcttaa cacatctaac gattcttgat atttttgaat aaaatttgta
    15061 ttctctgtca ttttctttaa caagaataca ccaattacta acatggcgat tagaaccaat
    15121 ccaattttaa agttaatgaa aaagcctaag gcaactgata aaacaggtgt tactatggct
    15181 tgagcactat ctggaatcaa gtgagcaatt gccatatgtg ttttagccgc gttgtcatca
    15241 attgtctttc gaatatatcc cgaagaattt aaatcaaaaa aacgaaaact tgcttctgtt
    15301 aaaccgctaa ttcccttttt tctcaaatta gtctctaagc gaaacccaag gatatgagaa
    15361 aataagccag aaaaaatata aaataaagct cctaaagtta accaagcaac agtttgaacc
    15421 gcataactct tagctaaata ttcatctcct acaataatta gagactgaag aaatttgtaa
    15481 atactgtaat atccatagac tgttaatacg acagaaaaat cagaaaataa agtggctaac
    15541 catccgtagt atttcatttt agggacataa ctaaataatt ttttgtatac tttcattttt
    15601 tcctcccccg cattcaattt cttctcttat tcgtttacaa ctgttaatgc tttccgtaat
    15661 agcgtaaatt agagatgtac aaacagataa aaggtaaact acgttctatt ttttgtattt
    15721 ttttaagact tttcttccaa tcaatacacc aatcatagat aaacttgcta caactaacac
    15781 tgaaatgatt ccccatatac ctaccgcata gtggatcatt gcatccattg ttgcagcaga
    15841 ttgtccttta tctatccaat cttgacgaaa acgatctgta aagaaccata taggaatcca
    15901 ctgtccggca gcactgccta attgcattag agcccaagac gcaacttgag gaattgctcg
    15961 atcatatcca aatacagata gaataatatc agctaagatc gccatactaa tcgaactgat
    16021 aaaccaaggt aagtacgccc cgcccattaa cgtaaaaaat aacaataaaa caattgtgta
    16081 aatagtaaaa attccttttc tagggtagtt atagctaata taaacaaata tgattccaga
    16141 taataatcca aaaatcccgg gggatatgct gtgaccaaac gccccaccaa tactactaac
    16201 cattattgaa aaaaagtaaa ttactaggga aataactgta gctaaaccaa taaaaatata
    16261 atcttttgtt ttcatatata ttctcctcaa ttctatttcg ttatgtcatt aaaaatctgt
    16321 tctatcaaag ttgaatcttc tactctaagt tttatatctt tttttattcc accatttttt
    16381 aaataaataa ttctattgca tgttttataa agaaattccg gatcatgagt agctataact
    16441 actgcaccct tttcttttaa gtctttaata aggtcagcaa tcctttccat attaatataa
    16501 tccaacccac ttgtaggttc atcaaataga aataaattag tttttgacaa tgaagctata
    16561 gaaaccaata atctttgttt ttctccaccg gataaatcaa acggtaattg atctttcatt
    16621 ttataaagtt ccattttttt ttaaattttt tcttgctgat ctttactaac tatttcattc
    16681 cccagggcta actcgctcct cactgattct gtgaaaaatt ggtaatccat ttcttgcatt
    16741 actaaaaacg gcgatgacaa atttttttta ttttcgatta tctttccttt ggtagggcga
    16801 ataatcttca tgagtattct tagtaaggtt gttttcccca caccgttttt ccctaccaaa
    16861 ccaataacat ctccaacttt gacatcaaaa tttatgtctt ttagaatatt ttcataagta
    16921 atttcttcaa cttttaatac atctgcactt accttttcga tatagggaac ttcaatcgaa
    16981 ctattaaaaa tgtcaaattt ccttaatgcg ttattgtttt cagtttttaa caactgaata
    17041 gttgtagttt ttacagttct atttttaaca aatagaactt gatcaattaa ttctttcaaa
    17101 tagtaaaaac gatgttcagc tataacaata gtaaatcctt tattttttaa atcacaaagt
    17161 aagtcagcaa gtttcttagt cgctttgaag tccaaatttg aagacggttc atccaatata
    17221 attaatttag agtttagcga taaactacat ccaatagaaa ttttctgccg ttcaccactt
    17281 gatagcgaaa aaatgttttt atctaatata tttgaaagat ctaataattc agctaattca
    17341 ttgattcttt tatccatatg ctcttttgta aatccaaagt tttccatcga aaatactaac
    17401 tcactcgttg tatttgttgt aaagaattgt cctctaggat cttgacttac gtagccaata
    17461 ttttttgata tactttcttg tgttaaggat tgtagcgagt tattttcaaa taaaatttcc
    17521 ccaaccaatt ttccgtcata aagtcttgga ataagtccat tcattaactt taatagtgtg
    17581 gatttcccac tcccagaaga accagtaatc aatgtaagac tgcctttttt tattgaaaaa
    17641 ttaaggtcaa caagcgcttg ttccttaaca tccttatatg aaaaagaaac attgttaaat
    17701 tcaaacaatt ctttcacctc aaatcaaaat acttaagaaa aaaatagtaa cggaaaacag
    17761 gatgatatag tctaataagc tcatgtttgt tttatagtaa gaacttcttt tcacaggaga
    17821 ttctatcccc tttgttattg cattagcggt catttcttca gcaatcatag ttgcacgtat
    17881 aagaacggga actaacagaa attccaaata ttgcagaggc tgaaaaaaag acaagcgaat
    17941 gtttctcatt tttaaagatt ctttaatcat tcttatttca gaacgataag tcggaaagaa
    18001 tctgatcact actgtaatac ttaacattat ttttttcggt aaatgtattt tttctaaaga
    18061 ggataataat tcattgctag tatagacact aaccaacaac catgagatca ttacgaccgg
    18121 caacattcga aatacaatat ataaggatgt cgcaaaaaat atagtaaaaa actctatctt
    18181 gatagcatca ataattttca ttactaaaaa cagaactgaa taaatcaaga tgaaataaat
    18241 acatgtcttt attttacgaa acatcaatac taaaactgta gcaataatga aacaccccac
    18301 ttgaaatata tttccctttc ctaaaaaaat aatgaccgga aaaaaaatat ttaaagtaat
    18361 caaggttaac ggattgattt ttgacactct ttcacctcca aaaaataaga tcgcactgtt
    18421 atcttaagct gataaaaatg caccgttatt cagataacag tgcgatctta tcaaaaagca
    18481 taactgagaa caattctcaa tgcaagcttt ttttaattaa ctcagtaatt caatccaccc
    18541 aggaacagaa aattgataca atcttctggt taatagcata gtcttttctt ttgaatagct
    18601 atgcttcaat atatcataga atgtagcgta gaatgagtaa atgtagaagt ggatttcttc
    18661 ttcattaaag actaaatttt gattgatcat tccgatagtc tttaacttat ctagacaaca
    18721 gactgtcttt ttcgtcacaa atgatgtaat tttttcaaca atatcttgat atttggttcc
    18781 agctgattgg aataaaatca aatcgaatat acgtttattc tcaaaaatat aattaataaa
    18841 tggatctaat tcattgaagt tctctctcca acttttctct atatctatat tttctaagtt
    18901 taagaggtat ttggtaaact cttcattata catattaata agcgcattaa tatgcggctg
    18961 aataatttct tcaaataacg cttccttaga agtaaaatgt ttataaaaag cacccgtagt
    19021 tacgcctgcg gtcaggcaca gttgccttat cttagtttgc tgaaatcctt ctttttcaaa
    19081 atggcttagt ccactttcta ttaacttttg gtgagttata atataatctc tggtttctat
    19141 gggtacttcc tcctaataag atgaattaac tttattatct tacacaggac ttgaagtttc
    19201 aatcgttaac gtttattact gaactcattc cttagagcta aactaatgag aaagagcaga
    19261 ataactgata caaaaagcaa tcctacaaga attaatagac tcatctaaat cattcacctt
    19321 ttctaaaata atccgagaaa gatttaaaaa aattatatcc agctattagg ataagtccta
    19381 tcataatcac tccaatagcc attttcttat cctccctaaa aataaagtct tttacttaaa
    19441 accttttttg taatcgtcaa taaatttttt ccctttatca gttaattgaa atcctatatc
    19501 taaatagtcc tcatatctaa gtttagccag catctcactt acagatgctg gactaacact
    19561 tagaactttt gctaaatcat tattttttaa tttctttttt ctttttgata attcctgaat
    19621 tgctagtaaa taaattagta tagtagtcgt tttaatttgt accataccta ccctccaaat
    19681 tatttcgaaa ggccttcagc aattttatcc aggttccatt tcatcatcgc atagtaacta
    19741 tcaccatctt gtcctttttc tgcaattgaa tcagtaaaaa tcgttgaata gataggaata
    19801 ttggtatctt ttgatactgt tttcattggt ctatcatcca cactgctttc tacgaataag
    19861 gagggaactt ttgttgtgcg taatttttca actaagtgtt ttatttgatc tggtgttcct
    19921 tcttcttcag tgttgatttc ccaaatgtaa gcagaaggca cattatacgc tttcgagaaa
    19981 tatttaaagc atccttcact tgtaacaatc atttttttat cattcggaat tgaagcaaat
    20041 ttagatttag cttctttgtc tagagaatcc aatttttcaa tatacttatc tagattttct
    20101 ttatagaatt ttttattatc aggatctttt tccgctaact gtttttcaat atttttagcg
    20161 taaataatac cgttttctaa atttaaccaa gcatgaggat cttccttccc tttctcactc
    20221 tgaccctcta agtaaataac atctatgcca tcacttgctg caaaatagtc tttgttttcc
    20281 tctttgttcg catttttaac taattttgta aaccaagcat ttcctccagt ttccaagtta
    20341 acaccgttat aaaaaatcaa atctgctttt gaagtttttt gaacatcttc aggcaaaggt
    20401 tcatattcgt ggggatcttt cccaataggt acgatactgt gtaaatcaat tttatctttt
    20461 gctatatttt cggtaatatc tgctaaaatc gaattagtaa ctactacttt taatttttcg
    20521 ttcgaagcca ctttatcttt gtctttacta tttattgaac atgccccgaa tataaaaaga
    20581 gcaatcaaaa tccccagact tcccagtact gctttccatt ttctcatcat aacgatctct
    20641 ccttctttct taaaaatttt tgttttggtg agattagaaa actaattaca aataagaatg
    20701 ccgctgttaa aacaatactt gatcctgctg ctatgttaaa actataacca ataaacaatc
    20761 caagcaatga tgaacttgct ccgactgttg cagaaataaa tatcatagtt ttcaagctat
    20821 ttgcatataa atatgctgta gctgctggtg taattaacat tgcaacaatt aatatagttc
    20881 ccacactttg catagctgtt actgctacta aagttaatag aaccatcaat agataatgat
    20941 aaaaactgac attcatccct attgcttttg ccattaatgg atcaaaagaa gtgattaata
    21001 attctttaaa aaatagaact actataacca acactaaaac cgaaacgcca attgtaatcc
    21061 acttatcaat atcttgtaca gctaaaatat ttccaaataa aatatgaaat aaatccgttg
    21121 agctatttgc aactcctata aggataactc ctagtgctaa aaaagaactg aaagtaatac
    21181 caattgctgt atctcctttt attacactat tgctttttat aaaagtaata atgatagaag
    21241 ctaatagacc aaatacaatc gcaccaataa aaaaattaat acctaaaata taagaaattg
    21301 ctactcctgg caaaacagca tgagaaatag catcacccat aagagacatt cctcttaaaa
    21361 taataaaaca acctactgtt ccagaaacaa tccctataac tacagatgta attagagcat
    21421 tctgtaaaaa gtgaaagtca tacagaccat taataaaatt ttgaagcata ttattccccc
    21481 tctccaataa atatagtatc tccataggct ttttttaaat tttcttcatt aaatacagat
    21541 tctacacttc cgtgagcaat tagatttcga ttcaaaagaa taatattatc aaaatacttc
    21601 tttaccttgc ttaaatcatg atgcacaatt agcactgttt ttccctgatg tttcaatttt
    21661 tttaacgtat ccataattat tcgttcacta actaagtcta tccctacaaa tggttcatct
    21721 aaaaatatga aatccgcatt ttgagcaaga catcttgcta aaagtacacg ttgaaattgt
    21781 cctccagata attctcctat ttgatgatta gaatattttc ccatatcgac cttttccaaa
    21841 gcctttgaaa ctctttgcca ttctgcattt ttaattcttt gaaaaacttt cattccggca
    21901 tatgttccca tagatacaca ttcttttact tttattggaa aagtaaaatc aatatcagct
    21961 ttttgctcta catatgcaat cttcaccagt tttttattga tatcttcatt attaatggag
    22021 acctctccct gatgagggat aatatttaac attgctttaa gcaatgtgga ttttccagca
    22081 ccatttggac caataatacc tgttaaagca ccttcttgta tttttaaaga aatctcgttc
    22141 aatgctaaaa aatcgttata acaaacatta agctttttca cttctaacat aactttctcc
    22201 ttctaaatga taatgattat cacgatcata tggtatacaa aaattatatt ttagtcaacc
    22261 ctaaatttta tagtttagat ttttttctgt tgctgaagat tagaatgaca aaactatttg
    22321 aaactacgcg tatagtaagt agctttcaaa atatataaaa aaggagcgtt ctcaaatgag
    22381 aacatgctcc taaaactact ttattggcgt atggaaaaat tatacttgaa gtggatagcg
    22441 ggttgaataa gttgataagc atcaaaacat ttataaaaat tattcaagac tatgtctgtt
    22501 ccatatacaa atatgaaaat cagtaaactc catttaactt ttttcgtagt gtgtcttttt
    22561 atacatgttt atcgttatta atagtgaata ggaaaaaaac tatttttgat cattgacaat
    22621 taaatacaat ttttgaataa cgatccctgc tattcgagct agaaactcaa gtacgccatg
    22681 acctgattag cgacaaatac ttagagattt ctttttgttc tgggaaaaca aaatgccatg
    22741 acaatagcag caaataatga tacttccgta aggctcgccg taaactgggg agaggtgaaa
    22801 ttcctatgtg cttacccaaa gcaattcaaa aatttcataa ctactatttt aggagatgat
    22861 tttatagaat ccagagtcta cctgaataaa aaacaaacct gtgcatttct aggaatcgat
    22921 aataatactc tagataagtg gattcgtata tataatttcc cagtaataag agttggtggt
    22981 gtagttcgct ttgacgtaga agatattaaa gactttatga atacatttaa aataacttga
    23041 aattttcttc tcgccgcata tactaaaatt agtgaaatat actgccttac tttttttaag
    23101 gagtgaggat tttgcctaca aagctttcaa atgggaaata taaaactaat ctgagatatc
    23161 caaaaaggtt taaagaaata acaggaattg catcagaaaa gtatcaaaaa acatttccaa
    23221 ataggcaatt ggcgattaaa gccgaaaatg atatgaagaa gaaaatagaa aaagttttga
    23281 gagaggagaa tgcaaactca ttagaattaa aaggaaaaat aacgtttaaa aaattttacg
    23341 aatctaaatg gcttcctaga tatgaattag gtcaaacaat tagatcaaac aggcctccta
    23401 gtgatataac cattagtaat acaaaagata tatttcggtt gcatatttta cctatgtttg
    23461 gtgaatacgc aatgaattat ctaaatatca atactgagat aatatcagat gaattaacca
    23521 aaaaatcgaa agaatatgca aatataaaaa tcataaaagg atatgttcgt tccatgttcg
    23581 atattgcaga aatactaaat tatatcgaat ttaatcgaac tactaaaatt attcaaagta
    23641 tcactgcacc taaaaaaaat gctttagaag aaaaaagaat acaagaaggt aagcaagcac
    23701 tatcatcaaa agaacttact aattggatag aagcagtaaa tgatgatttt aataatcatt
    23761 tacttacttt tcatgattac acacttttta tgattaccct gtatcttggt gatagaaaaa
    23821 gtgagactta cgctttgcaa tggaaatata ttgattttga aaaacaaacg gtccgtctta
    23881 aacatacttt agataaatat caaagaaaaa agtttacaaa aggtcgaaaa gataccgtca
    23941 ttcaagtacc agaagttgta atgacattgc taagtgaatg gaaatcggta caagctgatc
    24001 aattgttgaa attaaaaatt aagcaaacgt tagatcagta tctctttact tatactaaac
    24061 catctggaga agtaaattgt cctgttcatg ccgattacct aaactatcga atcaattcaa
    24121 ttaaaagaag gcatcctgac ttagttcatt tatctccaca caaactccgt catacttatg
    24181 caactatagc tagacaaggt ggagcagata tgaatcaaat ttcaaatgct ttaacacact
    24241 cagatatttc tacaacaaag atatatgtga acacaccaga tatagttgat aaggctgttt
    24301 ttgaagcttt tcagagagga ttgaataagt gtgattgata tgtgttctta aatgtgttct
    24361 taaaaacaaa agaagcactt tccaaataat tgaaaagtgc cagaaacgtt gatatataaa
    24421 ggatattaac gttttgagaa ttgtgaagct ttacgtgctt tcttaagacc tggtttttta
    24481 cgttcgacca tacgtgcgtc acgagtaagt aagccagcac gtttaagagc taaacggaag
    24541 tcaggatcaa cttctagcaa tgcacgagcg ataccatgac ggatagctcc tgattgtcca
    24601 gcgtatcctc cgccgtttac gttaacaaat acgtcatatg cgccttttgt ttcagtaaca
    24661 gcgaaaggtt gattgattac ttcacgcaag tcagcatgtg gaatgtattc ttcaacatct
    24721 tttttattta cagtaatttt gccagttcct ggtactagac gtacgcgagc aactgcgttt
    24781 ttacgacggc ctgtgccgat atattgaact tgtgccaatg aaattccctc ctattagatt
    24841 aagtttgtga tgtctaaaac ttctggttgt tgtgctgcat gtggatgttc tgctccaccg
    24901 tatacgttca attttgtaaa ttgtttgcgg cctaaagtgt ttttaggaag catacctttt
    24961 acagaagttt cgatcaaacg acgagaattt ttagcacgca attcgccagc agtgattgat
    25021 ttcaatcctc ctgggtagtt gctgtgacgg taataaattt tgtcagtcgc ttttttacct
    25081 gttaatttca ctttgtctgc attgatgacg atgacgaaat cgccagtatc caaatgaggt
    25141 gtgaaagttg gtttattttt accacgtagt acagatgcaa caactgttga aagacgtccc
    25201 aatggaacat cagttgcgtc tactacatac catttacgtt ctacttcgcc tggtttggcc
    25261 atatatgttg tacgcacgtt attttcctcc aatttaagtt ctgatgtttg acatacactt
    25321 cgagtttccg gggctctttg tggggcaaac aataccattt aacatattac ccgctaatct
    25381 agtcaatgtc aacgtttttc tgcttgattg aaaaatttat atgaaacttt acttcttttc
    25441 tttcttttat ataaaagggt cgtttcctgt caaaatgaaa aacaaaaaga ggctgtgaca
    25501 aaagtcttgt cacaacccct tattttgaat aaacggtgtt ctcctgccaa ctcctcggtc
    25561 acaagtcaca atatccataa aacataagaa gctgtttttt aaaattgttt cttgtgactc
    25621 ggagctgaac ggcaggctta caacctcttg gtaaacggtg ttcaatcagc tgaccctcgg
    25681 tattagctca aaaaacggca aaatatgagg aactattttg acgtttgttt ctctaatact
    25741 caggtcaaaa cgctgatttc acaacctctt tttcggtcaa gttcaacaac agtttttacg
    25801 cgtcgatctc tggcgctttg cccaaatgag cttggatcat ccagatacgt ttttctgtgg
    25861 ctgttttgaa ttcgataaag atatcttgtg ttggatcgtc gccttcttct ccagaaactt
    25921 cgattccttt ttggtaaaga tctgctagat aacgatagcc tgcaacaagt gtttccaaac
    25981 gttctggaat agtacgatcc cagcggccaa tttcatcagg gatttttgtg ttatctgcaa
    26041 attcacgcaa tgtagaataa ggtgaaccgt ctaatgtgat caagcgttcg gatacttcat
    26101 ctaactgagc gttcaaatca tccatgaact catccatcat tgggtgcaat gtaaggaaac
    26161 ctggtcctct catgtaccaa tgtgtttggt gaacaacaac agacatttgg cttagatctg
    26221 caactaattg atttaatact tctttggttt gttggaattt catctttctt gcctcctaac
    26281 attttcttac acttaaaata atagtagact ttcaatgaaa agtacaacaa aatcatttac
    26341 ctgagaacga ttttcagttc gcttttttat ccgtaaagcc aatcattttt cagaatcagc
    26401 taattcttct cgcagttgag caaattgctg ataagtctgt gctaaatgct tttgtgtcga
    26461 aacaaataat ggatagattt tctgatattt tttatgatca gtttcttgta ctccaacttg
    26521 ctctttcaat tctatcaatt cctgtgcttc actgatactt tggatctttc cgatgcttct
    26581 ccagcctaaa atcacagctc ctaatgcgga tgcttctgtg acagtaggga aaacaagcgg
    26641 gtaacccagt acatcagcca tcatctgacg aaaaatcgga gattgagcaa acccgcctgt
    26701 agcacggatc tctgtgagct gattttcctg gcaaactcct tttaaaatcc gataaaggtt
    26761 gagacagatc ccttctagaa tcgccttcat catttctttt tctgtatgtt gccgctgtaa
    26821 accgataaaa ctgccaaacg catctgcgtc ccaaagcggt gctcgctcac caagtaaata
    26881 gggatgaaaa atcagaccat tggcacccgc tggcacaaaa gcgatagccg aaagcagtgc
    26941 atcatatgga ttttctcctt tttctttagc agattgaacg acttcctgta aaaatgtatg
    27001 ccctgcccaa tcaaagatgc cagcaccatt gctagtcgct cctcccacta cccaatgttt
    27061 ctgatccaga acataacaaa acgtctccat ttctggatgg aactgaggct gagtagtaat
    27121 aaaacgaagt gctccgctcg ttcctacggt caaagcggcg atcgtatcac caaaagcacc
    27181 taaccctaga ttagaaagcg gcccgtctgc ccctcccaaa acaaatgata tatcatttgg
    27241 caaacctagt tctgctgctg tttttgatgg tagacctatt acttgatagg tggaaggaaa
    27301 gacttgcggc aattgctcta ttcgaatgtt caagtagtca agcacttctt ctgcccattg
    27361 gaattcatgg atatcaaaat atccagttcc tgaagctgtt ccgtagtcgc tgatcagttc
    27421 gccaaacaat ctataaaaga tatagtcttt gataccgaca aaataagcag tttttttgaa
    27481 taattcaggt tggctgtttt gcagccactt gattttgttc aatggtgtca ttggatgaat
    27541 cggcgtccca gtctgccgat aaaattcttt gccaagcacg gtatatttca gtttttctgc
    27601 ttcgtccgct gctcggttat ctgcccaagt gatcattcga gataacggtg ttttctcttc
    27661 atccaacaat atcaggccat gcatcgcaga ggagaaggag acaaattgaa tgattgtgtc
    27721 tgaaggcaac tgtttaatta cttcttttat aacggtgcag acaccttgaa agatcaattc
    27781 ctgatcttgt tctgccatcc cttgtgtttc ttggatcaaa gggtagcgaa aataagcgga
    27841 tgctgcttct tgggcttcat ctgtgaaagc aactgctttg gtttgtgtgg ttccgatatc
    27901 taatccgatt gaaacgttca ttttttcacc tcaaatttta tgtatgtaca aaaatttagt
    27961 ttaagcttgc gtatcttggt tttgaaaagg gggagatggc gtgttacttt attttatcat
    28021 tggttgctgt atcggatcat ttttatgttt aaccgcccag cggattccgt taggtcactc
    28081 tattatttat cctagatctc attgtgtcaa atgctgtcgt tcgctttctt ggtatgaatt
    28141 gattccgatt ctctcgatca tcgtccaacg atttcgctgc cgctattgca ggtgtcggtt
    28201 gcccttctac tatttgttgg cagaagcctt atgtggtggt ctttttgcct ggttctttac
    28261 tttttcttcc gcaagaaatt tttcgacgtt catatggatg ctttcggctt tgctattttc
    28321 gttgatggat ttattttact tcatggtcca ttctcacacg ctattctgtt cttgggcaat
    28381 tttatggatt ttttggttgc aggccggcgt cttccaatgg caaagtgtgc tgttaacgtt
    28441 tatcgtaggc ggtgtgtgcc ttcgctatgg tcaatcctat ctcggagctg gcgatactct
    28501 gttgatgctt tcttggagtg gtggcttgtc tctagaagag ttgcttcaga tcttgtttct
    28561 tgctagtctg tttggaatat ccgttttttc tgtttacttt atctgtcata aaaaaaagtt
    28621 ggaaaagctg cctttcattc cttttttgag cagtgcgttg ctgattgtcc ttcatcttag
    28681 gtattgaaaa aaggttgtga caaacgactt gtcacaacct ctgatatata gtgttctctt
    28741 accaaatcct tgtgacttgg agctaggcga caggttcaca acctctccac taaatggtgt
    28801 tcaatcagct gaccctcagt attagctcaa aatgctggtt ttacaacctc tgatatacgg
    28861 tgttccataa gtagcttttt cggcttctta tttcttgacg ccccaatgtc gacgaaggaa
    28921 acctgcacgt ccagaacctt ctcgataaag attgtaatgg agcttgtttt tcttgtaata
    28981 atcctgatgg taatcttctg caggataaaa ttcgcttctt ggttcgattt ttgtcacgat
    29041 tggcttatcg aatcgcccgc tgtcttgcaa tgcttgtttt gaacgctctg caatatttcg
    29101 ttcttcttca tcattatagt agatcactgg acgataagaa tcaccgcgat cagcgaactg
    29161 tcctaatgcg tcagtcggat cggtttgctg ccaatagatt tccaccaact gatcataaga
    29221 aataacttct ggatcatatt cgatctctac tgcttctgta tgccctgttg tgcctgttgt
    29281 tacttgttca taagttggat ttggtacatg cccgccagta taaccggatg tcacagaaat
    29341 gatccctggc tgctcttcaa atggtttgac catacaccaa aagcatccgc ctgcgaaaac
    29401 tgcttttttt gtcatataaa atcccccttt tttctcatca taccttgatt tccaaaagga
    29461 ggataacgtt ttgcttgtga cagaaaaaac ggaaactatt atatacgtga tacggcttct
    29521 gataaatgcc aaaacgaacg tacttgatca aaaacggtta aatcaggaat cacatattca
    29581 gcagaaggaa gatttatgtt ttcacctatt cccaatacat gcatgttacc agccaaagct
    29641 gcttgacaac cagcttctga atcttcaatg accaagcatg catttggcgg tacattcaat
    29701 tgctgtgctc ctttgagaaa aacttctgga tcaggttttg cttttgatac ttgcgtgccg
    29761 tcaataagga catcaaaata acttgtcaaa ttcgtttttt ctaaaatgag gcgtgcattt
    29821 ttgcttgctg atcccaatcc gatcttgatg tttgtttttt tcaagtactc aagaacagct
    29881 attgcaccag gcaaaacgga tgtttcatcc attttttgaa tcgcttgaag atatagattg
    29941 tttttttgct cagcaaaggc tgatttctct ttttctgaga atttctgttc catttttcca
    30001 aatgacagca atcgctctag tgactcggtt ctggatattc ctttcagttg ctcgttttct
    30061 ttctcagtga atggaatgga cagctggtta gctaaaacca gccacgcttc gtaatgataa
    30121 cgagctgtgt ctacaagaac gccatctaaa tcgaatagtg ctgctttgat tttcatcgat
    30181 tacctccttg aaaatgaacg acttgaagta aagatgcgat gtttttagct gtgcgatgaa
    30241 gattatcagt ggcttgctgt aaagcttctt gtaaaggcac agctgcagga aggatcggaa
    30301 aaacggccga aatgccaaca tctgctgctt tttcgcttcc tttaccaaca cttccgcaaa
    30361 tagctatcac aggaacgccc tctggtactc ttctagcaat cccaactggc gcttttcctt
    30421 ttagactttg tccatctaga cgtccttcgc ctacgattac gaggtctgct tgctgtaccc
    30481 gtttatcgaa ctccagtaaa tccaatacgt agtcgatgcc agagactatt ttccctgaag
    30541 caaatgctaa cagtcctgca cccattccac caccagcact actgcccgat gcctcgatta
    30601 cttgaggggc aaatctcata taaaaatcat gcatggcttg gtcagccact gctaattcat
    30661 tttctttcaa gcctttttgt ggtccgaaaa caaaagaagc gccttctttt ccgcataaaa
    30721 gattagtaac atcagtaatg acagtgattt tggctttaat cagctcaata ggcacgttcg
    30781 ttttatcaac tgtagcaatt ttacctagat tttctcccag tgcttctact ttttgattat
    30841 cttgatcgta aaaagcataa cctagacctt ctgccatgcc gatgccgcca tcgttggtac
    30901 tacttccgcc aacgccgatg atgatgtgtc gaacaccttg ttgcaacaat tgtcgtatca
    30961 gttctcctac tcctcgcgtg gataacctaa gtggctgttg ttggtttagt gggacatctt
    31021 tcaatccgac gatttctgcc atttcaaaaa tcgcagtttg accgtctgaa gcgtactgga
    31081 tttctttgtt tttgccaaat gcattagtaa cgactgctgt ttgccgcttc aaccttaaac
    31141 tgtctgctaa tgatgaaacc gttccttctc ctccatcgcc tataggtagc agatcatatt
    31201 cagcctgaac aaaaacttct tgaaaacctg attgaatggc ttgtgctgcc tctaaggctg
    31261 tcagactttc tttgaaagaa tctggcgcaa tcagaatacg cattattttc ccctccgttc
    31321 caagatcagt tctttttcca gctggtattg gtctccattg acaaaaatag gtaatgtttc
    31381 tttcgtctct ttttttatta ctatttgttt ttttgtcaaa acaaatagca acctgtgatt
    31441 tttccaaata aaaggaaaag aaattttttc ccatgctgaa ggaagtcttg gagaaataga
    31501 aagagttcca ttcgttactg taatacctga aaaaccaaat ataactgcta gccatagtgc
    31561 tcctaatgaa gcagcatgaa ttccgtcatc tgatgaatgc ggatctggtc ctaaatcaat
    31621 caggcacgct tcttggaata aatgatacgc ccattccatt tcttggatac ggcaagcctc
    31681 tatggcgtga atcgctttac tcaacgatga gtcgtggatt gttttgtttt catagtaatg
    31741 caaattctta gaaacgattg gttgagaaaa atactcaggt aataagtata acagcatgac
    31801 aagatctgct tgcttgctga cttggagttc atttacttcg ctacgggcat aatcaagtaa
    31861 aatagactgg ctgccaggac gttctttgta agtggtcaaa tcaataactg gcttactgag
    31921 atacgtgtca tcttgcggaa tcagctgttc tttgttaggc actggcaaat ataacttatc
    31981 taaaaaattt tgacattctt tcaaaaaaag cggatcgcca gcttgatatt taacagctaa
    32041 ggccacatta taatgcgcca gatagttcgt atatgcatta ttgtcgatat attctgtgta
    32101 ttcatctgga ccaatgactc tgtgtatctc taaatgatca tcgactttta cagctctgct
    32161 cagccaaaaa gctgctgttt catgaatgag cgtttttccc tttgtttcta aaaacgattg
    32221 atcctctgtt gcttggacat actcttgaac ggcataagca aggtctgcac tgatatgatg
    32281 ttctgcggca gctgaagcaa cttcttgtct tgttccggtt ttgatattca atgcagcaaa
    32341 tttgggtgtc tcttcctgac cagtgtgtgc gctttcccat gggaaaagtg caccttgata
    32401 gtgataatct cttgcttttt gccgtgcgcc ttctaatcga tcataacggt acagcagcaa
    32461 attctttgct acttcaggaa actgatataa aaagaaaggc aggataaaaa tttccgtatc
    32521 ccagaaaaca tgccctttat aaccttcgcc agttagtccc ttcgccccta cactaacact
    32581 tggatcattg cggggagtca taatctgtaa atggtaagca gcgaagtcca tcgctaactg
    32641 atcgaaagaa gaatcgcctt ggatcgttat ccgatgctga tgccaaaaat ctgtccaata
    32701 agacatcgac tgctctaaaa tttctgtata actaacgctt ttctgggctt gggcaatact
    32761 tttcttaaac gggtctgttt gctgaagaga ggtagagagc acgatgatct tctccagcgt
    32821 aaaggacgta ttttttctga tcttttctga aaaggaagcg gtgatccggc gattttttgc
    32881 ttggaataca cctgctttat tgaatttagc tgcaatccca atatcaattt tactttctgt
    32941 tgtctggtag gatgcactga tcaaatcgtc accaaatact cgcagttctt tttcaaccaa
    33001 ctgctgtgta ccaaaattgg tttgctgtcc atcgataccc gttttcacat ctacttggca
    33061 gtcttcggtc aacggcttga tgaccacttt cagggctgcc tgatggagat tattttgatc
    33121 agcaacacga caagtaatga cttccacttt ttttccttga gcagtttcaa tgagcgcata
    33181 gcgtttaagc tctcccattt ctaaatctag tgcccgctcg tatgcaagaa ctttcatcgt
    33241 ctcaagagaa aaatgttcac cgtcgatttt tatttcccat ctgatgacat ctgggagatt
    33301 cactaactcg gctgattctg ctcctaaagg tcggttatat acaccagctg caaacattcc
    33361 ccttacttga ttccgatagt cttcctcctg gcttcctctg ataccaaaaa atccatttcc
    33421 aatggtcatc tgactcgcat acttattgat atactctaaa tcaaactgtt tttcttctac
    33481 tttcataagg taataacttc tcctgtttct gccgaacgat ataaattttc caccagattc
    33541 tggatgacat atccttgcca gccatctgca atcatctgag tctggtcacc gagcgcccga
    33601 ttcacaaaat gagccatact tagttcatgt gggtcattaa ctggcgtggt cttttgtaag
    33661 gaaaccagct ttccatctac atcttcgaat agttcgcctg gatacagatt agcacctgcc
    33721 tgatcgccac aaaaagccac attcagtttg tcttctctga tattcaaagc aaacgaagta
    33781 ttcagttgga tgatcttgcc gttttctaat tccagtgtgc caaaaattgc atcttctact
    33841 gtgaatttgc ttggatccca tttcccgaat ttccctgaat tttttttcgt accaattttt
    33901 tggaagctgt acgcccgaac gctttttact ttaggaaatc cagtaatata catcgctgta
    33961 tcaagcatgt gaatgccata atcgatcaaa ggcccgcctc cttgaagttc tttgtcgata
    34021 aacgttcccc agcctggaac accagaccga cgaagcgcat tcgcttctac gtaataaata
    34081 tccccgagaa catcaagatg attcttgatc attgttgctt cgtcagaaaa acgatgctgg
    34141 aatccataag ccaaaatctg cccgttttta tcggctgtct ctgccatctt tcttgcttga
    34201 tcagctgtca tagctggtgg tttctcgcac atcactgaag cattagcttc gagggcagcc
    34261 aatacatgtt cgaagtgaaa gcgattgggt gtacagacac tcacgatatc gggagaaatc
    34321 tcattcagca tttgtgtaac tgatgtatac gcatgagaaa atcctgtacg tcggcaaaag
    34381 tcttctgctc gctggctgac tggatcaacc acagcggcca attctaagac atctgatctt
    34441 ttttgataat aagcagcatg tactttttct gctacttgtc cagcgcctat gattgctact
    34501 ttcttcattt atttctccct cctttgctta tttacttatt gttattcttt ctctattctt
    34561 tctctaaatc atttaaatga cgcgctgttt ccagacagtt tttcaagtat tgcaatgatt
    34621 cctgataaat tttcacttca ttttctcctc gcaaacgaca ttcatatacc atataacctt
    34681 catactcatt ttgaagtagc gtttgaaaat ggcgagtaaa atcaattgcg ccagatcctg
    34741 gttggtagcg atgattatct gctaaatgga tatgacccac atactctttt tgcgtagtca
    34801 atgcttttga aatatcatct tcttcaatat tcatatgata aaaatcagcg atgatctgca
    34861 catgtttcaa ttgattagag tcaatgtaat gacgcgcatc cgctaacaaa ttgatcatat
    34921 ggtcttggta tcggttcaaa ggctccaaat aaataaccgt acctgtttct tcggctactt
    34981 tatctaaata acgaagtgat tcgctgactg ctttatagtc tccttctttg cttcggggtg
    35041 aaaccatcgg cggtaatcga taagtgaaca ttccccaagc tgctgggata acgattcctt
    35101 tgcctccaac ctcgcttagt gcacgcaaaa tctcagcaat ttgacgtaca ccattaagac
    35161 gccgttcttc gataaagtca ccgatccacc catcgtagcc gttacaagct gtcactactg
    35221 ggatccctgt ttctttgatt gcctgtttga ctgtgtcgat attttctatt aacaacttac
    35281 cgtcgatttc gtattcttca aatccacagt ctttgataaa tctaaatttt tctaaaatgt
    35341 ctcttgggaa aaatgcttga tcttgcgtac cgaatttcat tgttctcctc ctcggacttg
    35401 aacgcccatt ttgatacttt cttctgggtg ctggtctaca taacgcatgt aagcttctgc
    35461 gctttctgca aatggcacaa ctggatcaat gatttcttca caatccaagt aaccgttcat
    35521 caataattcc caacatgtgt cctcgattcg tttacggttc catcttgggt aatctgggtt
    35581 tggttcacta gatgctcgag caaagacgat ttttgcatta ttgaaatgtg cttcgcgacc
    35641 aagattaaac ccagctggaa aaggtttagc aaaggcaaca taagaaatga tcccgccata
    35701 gccgatccct cttaaagaag cttgtaatgc ggcagcactg ccactcgttt caataactga
    35761 atctactcca cgtttatctg tcaattcctt tagacgaaaa ccgacatctg ctttcatagg
    35821 atctagcgtg taatcggcac cgtatttttc tgctaattct ctacgctgcg ctatgggatc
    35881 gatcccaacg atggtagtag cacctgcttt tttagctaat tgaatcgcaa tttgcccgat
    35941 agcgcctaaa ccgaagattg ctacagcatc acctgctcga acatgtgctt cccggatacc
    36001 acctaaagcg aattgagcag gatcgtaaca tactgcattt ttccattgat ccaaccgatc
    36061 aagtttacgt agtttataat tatcaacacc gtttgtaatc agcgtttcac gaatgggtcc
    36121 ataagtgcag actaactctc ctttttgata ctctgtcacc aaactgcccg tttcttcgat
    36181 cgtaccgaca atcatatttc ctaattgaaa ttcgccaaat accacaccgc gttggcttcc
    36241 ttcttctctt ggcaagaata ggcgccattc ttctgaaaat tcttcatcga taaatggact
    36301 taatcctcta aagtcgacaa tttctgttcc tggttttgga gtagcgaaaa ttactcggat
    36361 acgtacttcg ttcttttcga tctcacggtc ttcgtaatct ttgaatccgg ctattcttgg
    36421 ttctaatgca actaattttt tcatcttttc tttcctcttt cttttctttt tatcctttta
    36481 cgccgccttc gactaaatca cctttaataa atcgttcaga taacgcatac ataatgatga
    36541 ctggtaaagc ggtcaccaat gaagcggcca tcattcgtcc ccaaatgtag tctggtgtac
    36601 tgaataatgt gttcaaaccg attggtaaag taaatttttc ggaggtcgac aggaaaatcg
    36661 aagcaaataa ataatcattc cacgaaacca taaagcaata aacgaagaca gaaatgattc
    36721 cagaaatagc caatggtata atgatccgaa taatgatctg gatccggcta agaccatcga
    36781 tcattgctgc ttcttcaata tcagtcggaa tcgtgtcaaa ataacttttt agcatgaata
    36841 tcgcagtagg aagtgtttga acgaccatcg tgataatcag tgctgtattg gtatcataca
    36901 aacctagacc ggaaatgatt ttaaataatg gaacaacgat caaaataccg gaaaacatat
    36961 aaacggtgta aaagctagca ttgattttcg agcgagcttt gaagcgtagt tttgacaaag
    37021 cgtatgcccc gctgatccca atcactactg cagttacgga ggctatcaag gagatcacta
    37081 aactattttt gaaatacatg atgaatggaa aaatctcagc attgaaaata tcctgataat
    37141 gttcaaaagt ccattctttc ggaaaaatag tttgtgtcgt tgcaattgct tcttgtgaat
    37201 ccttgaatga tgtcatcagc atgatcaaaa atggaaacaa attgacagct aatagcaaca
    37261 aaatcgtcgc gtaaaataag attttcctgc tctttttatt tttcatttca agtccacctt
    37321 ctttcgtgag agcaagacga ccgctgcaat aatgatgaac aatagaatcg atatagctgc
    37381 tgcttttcca agatcattga acgcaaaggc tgtctgatac aaataaacgc ctaagatatt
    37441 gactttattc gtcagtagat agacatctgt aaacatatag aacatccaga tagatcgtaa
    37501 aacaatcaca gttgctagta ctggtttgat tgccggcagt gtcacgatct tgaacttctg
    37561 ccaagcattt gcaccgtcca tttcagctgc ttcataaagc gttttgtcga ctgtttgcaa
    37621 aatagccaaa aatgaaataa acgcatacgg atagtatctc cagatagcaa acaaaacgac
    37681 cataaagaag ctagcaactg gctggtcgaa ccacagcggt gcttcgttga aaagctgcaa
    37741 cctatctact aacagatcat tgacgattcc ataactatta ttgaacatat atttccaagc
    37801 aaaaatcaaa gaaatagatg gcgtaacata acttaataaa atcagtgaac gcaccgtttt
    37861 cctaaatctg aacttgcggt taagcaaaat agctacggct aatcctaaag ctgtactgcc
    37921 tgctacaact aagaatgtgt aagcaactgt taaacctaaa gaacgataaa attcactatc
    37981 acttaaaatt tcaagataat tgcttaatcc gataaaaacg gcgtctgagt taggattcag
    38041 cggcatatcg aaaaaactga tctgaatatt ggaaagcatt ggaatcagca ctaaaacagc
    38101 taataaaagc atgcttggtg ctaaaagtcc catggctaat tttaagtcag aatgtttcct
    38161 tggacgattc attgaaacct ccccctgata atctgccaat agctatctac ttttttcgaa
    38221 caccattggc agaacttctt gttatttcgt atttttttgt gcgtcagcta aagcttgatc
    38281 gacatcttcg tttccgacag ttacttggtt cactgctttt ccgatcacac ctgagcttgt
    38341 gatatctccc attttcgtga aattcttttc cccgactagt ccaaagactt gtacttcatc
    38401 aaaagcacta gcaatctcag atggcagctc gccaaaagac ttgatgactt cattttcttt
    38461 gtaaccatcc agttcgacta cttgtttatt cactggttga gcaccaccag gagacatcaa
    38521 gacccacttt tccatgtttt tcggttgaga taagaaagct gtgaatgctt cagcagcctc
    38581 tttttgtttc tcgtctagac cagaagaaat cgtcaatcca gatactgtgc catagacagc
    38641 ttgatctttt tgtgttggga tagcaaaacc gatattttct gagttccccg cttcataaac
    38701 agaaggcaaa atgtaagttg aatagattgc cattggtgct gttccattca tgaaagcatc
    38761 tttgatttct gtcacatcat tagagcctgg catcgtgtat tgggataagt tttgataaaa
    38821 cgacaatgct tctctcatct tttccgtatc gatcgttacc tcgccttcat catccaaaac
    38881 atttgctcca tttgacaaag caaattgaga gaacgcttgc tcactcattg ttccttcagc
    38941 agtaggcata gcaattccat attgtttatt ctctttatct gtgaaatgtt gagcaatttt
    39001 taaaatatca tcccaatttt ctggttctga aaaaccagct tctgataatt tttctttgtc
    39061 ataccaaata ccttgaaccc agccgctaat tggtgcagca atatagcttt ttccatcttc
    39121 tgaacgaaca aggtttttgg ctccgtcata ataattatct tctccagctt cctcgataac
    39181 gttttggact gccgtctgat caatcagttc atctttatcc atcactttag caaaatcctg
    39241 actgacttcc attacttctg gtaatttacc ggaacgagct aacgtaacga tttttgtatt
    39301 gaacgctgat tcttctaccg gcacttgctt gattttgatg tctggatttt ctttttcaaa
    39361 atcagcaacc agttcattga tcacacttaa tcgttcctgt tcgacagaag agtgcatgaa
    39421 ttgaatcgtt gtctgttgtg attcgttaga gctttgactt tcaccacaag cagtaagaag
    39481 aaatccagta gctagcagac aggtagtaac aataattttt ttcatttttt tccacctctt
    39541 attttattat tttcagccac atatattcat acggctggaa tatcaacttt cgttcttgac
    39601 tgcaatggat aatttgtcca gaaaacaggt ctaggtaatc tccttttggt aaaagataac
    39661 ctgctgattg attggaaaaa ttatggataa ctaagacttt ctcttccttc gcttcgcgaa
    39721 taaaactgat aaaagaatct gctgagtcca gtaagtaaat caaactattc ggatggaagg
    39781 ctttttcttt ttttcgaata gatagcatct gggtcaattc accatatact gctgttctta
    39841 atggatctgt ctgcaactct ttctcaatat ctgctaacaa aaatttttgc cgattgattt
    39901 ttcgattctc tcctgttttt tctacgcctt tgtaatcatt tcgacttccc agcacacttt
    39961 gaatataaat tgccggcacg cctggtaacg ttaccaaaaa tgaatgagca gctaaaaagc
    40021 gtttgcttct ccattccgtc gtatctgttg gagaacttaa cgcatctaga tacgtagcgt
    40081 ttatctcata tggactggtc gttccatctg ggtttttctt gtagtttact cgggtaccct
    40141 ctttcttcag ctgttccact aattcaagga tcgttttttc cggcagaata cctcgtaccg
    40201 gattcaatcc gataccgtca tgggaggcta aaaaattaaa aaaagttgtt tttttcgatg
    40261 cgaaatcgat ggtttttaac cattctttca acttacttgt attacccgaa cgaatcgctt
    40321 gaagggtcaa aggtggtagc gaaaactgat agaccatctg tgcttcattc tcgccattcc
    40381 caaaataact gatattatct ttgtgtggca catttgtctc agtgatcaat accgttcctg
    40441 gagctgcttc atcagcgatt gcacggaata attggatgat tttatgcgtt tctggcaggt
    40501 gtatacaaga agttcctagt tctttccata aaaatcccac agcgtccaag cgaataaatg
    40561 aagcgccttt ttctaagtaa aacaaaagaa tatcgatcat tttcaataaa acttctggat
    40621 ttttaaaatt caagtctact tgatcttcgc taaaagtcgt ccatacgtgt ttttctttcc
    40681 cgtttttgtc aatgaacgtt gtaagtaacg ggctggtcct tggtctagtc acgctgcttg
    40741 tatctgtatc cagtggcaaa tcaataaaaa aatctttgta cgtatcattc aagtttaaat
    40801 attcctgaaa ccaagtactt tcggcagaca tatgattgca gacaaagtcg aacattaacc
    40861 gtgtttcgct tctcatttct tctatatctt cccatgtgcc caaacggtcg ttgattgcct
    40921 gataatttac aacggagaaa ccatcatcag atgtatatgg gaaaaatggc aagaaatgta
    40981 caactctaaa tgtatctgcc aaatactttt gataaaattt cttaaatgta cgcaaagttg
    41041 gttgctgctc ttcaaaaaat tgatccccat aagtaatcaa gaaaatatct tcttcgtccc
    41101 aatgcagttt gcctgcctgg cctttcttct gctgataaac actgactttt tgttcaatca
    41161 tagaggcggt tttctcagac aaatctgagt cgcgataaat cgcttgtaaa agttttgtat
    41221 acatcggatt cccctttctt ttttgggagc gttcccaacg atttgatcat attatggaaa
    41281 acgcttactg tcaatagcat ttttttaaat aattttgata tacacaaaaa tacaaataag
    41341 aaagcttatg tatcaataat taatagcttc ttaaaaaaat atggtctctc atgatcatgc
    41401 aagctattcc taaattttca aaatcataga caaaaaagaa tgtctattgt actatggaat
    41461 tattctaaaa cgctcccaaa aaggaggtgc atataaaatt gaaacctact atttatgaca
    41521 ttgcgaaaaa agctaatgtc tcaaaatcaa ctgtctccag agtattaaat aaccagccaa
    41581 atatctctga taaagcaaaa gcaagtgtac ttcaagcaat caaagaattg aattatgaac
    41641 cgagcaaaat tgcaagagga ctgtcaggaa atggttttga cgcgattcta gcattatctc
    41701 atcgtacttc tcatacgacc tatggcaatc cttttttttc agatgttatt caatccattt
    41761 ctgccgtttc tgaacaaaat aacttcgact tgattctaca aacggcaaaa acaccagaag
    41821 aagagctaga aaaatgcgcg aaaaagatcc aagaaaaaat gatccgagga attattattt
    41881 taagctctac gatggatgaa acacttcttt ttgaattgga tcaatacaat attcctattg
    41941 tggtaatcgg aaaagtcgga aacgactaca cgaacatcta ctcagtggac acggataatt
    42001 tccaggatag ttacgatttg gtggaaacac tgatcaagaa tggacaccgt catatcggct
    42061 gtctccatgc tccgacagaa gtttatgtat ccaatgatcg tctcaatggt taccgtcaat
    42121 gcttatttga tcaccaccta gatatccgta atgaatggat tatagacggc ggctttcgct
    42181 tagaagatag catggcagca atgaatgatt taatgaagct acgagaaaga ccaactgcga
    42241 ttttttctac cgatgcatta aaatcattga gtatttacaa agtggcagaa caatctggaa
    42301 agcagatacc tgatgatctt tcgatcgcag gattcagtga tcccttattg tcctccctat
    42361 tttcgccaca gttgactcgg atagacattc cgacaaatca gttaggaatc aaagccacag
    42421 aacttttatt ccaactaatc caccaccaat accctgaaga aaaaagaatc attctttcaa
    42481 ccgagattac tctcacagat tcgatccaac aacaagaaat gtagaacaga attataaaag
    42541 aacagaaaca ctgaggataa gtcagttact ctgttctttt ttcaatcact catccctcgt
    42601 taccccctcc cggtatttta gcgaattttt cttattttcc atctgtttta gatagctgtg
    42661 gataactata atgtttgtgt taaactattt tttccacagg gaagaaaaaa ctatttttaa
    42721 ccacgttgtg tataagtttt tatagttcgt ttttttaaac acatttaccc ttatttcaat
    42781 ccatgttatt tggggatact tatctctaaa acataaatag aataacagac atgtctactg
    42841 gttttgtttt attcgaggaa ttattcaata atagctagca aaatattaaa aataaccgct
    42901 ctatcttccc atcaatttat tttgcattca aaaaatgatc tatctttact tttgttgatt
    42961 gacttctctt ggaagatgat cgttccgata ccttaattgg atacgaaccg atgttgttaa
    43021 gatcctttcc cacttttctt ctagttagac aaattatttt agacaaacca aaaaagaggt
    43081 tgtgacaata atttgtcaca acctcttttt taaaatatct attttgtttg attgttcatt
    43141 gcgtcttctg cagccatttg cgcttcaata tctgaaatac tgtagacatt ttcgcttgct
    43201 acaccgactt cttttggttc catgtcacga taacgttgca taccagtacc agctgggatg
    43261 atcttaccta taataacatt ttctttcaaa ccaagaagag gatctttctt accgcggatc
    43321 gcagcatccg tcaagacacg agttgtttct tggaatgatg cagcagataa gaaactattt
    43381 gtttctaatg atgcttttgt gatacctagt aagacaggtc gagaagttgc aggaacgcct
    43441 cctgagacta gcgtattgta gttttgatct ttaaagtctg cgatgtctaa taatgtacct
    43501 ggtaagatat ctgtatcacc tggatccatt acacgaactt tacgcagcat ttgacgaacc
    43561 attacttcga tatgcttgtc gccgatttct accccttgca tacggtaaac acgttgtact
    43621 tcacgaagca agtagttttc aacagataat acatcgcgaa cttgcaacaa ttgttttgga
    43681 tcgattgatc cttctgtcaa tggtgcacca cggtggatca tatcgccttc tgctactttc
    43741 atacgtgctg tataaggaac agtatacgta cgtgtatctg tttttccttt gattgttact
    43801 tctttggtac gtgttgcagg atcttctgag atatcgatga catcccctgt tacttctgtg
    43861 atcactgctt gccctttcgg attacgtgct tcaaagattt cttggacacg aggaagacct
    43921 tgagtgatat cgtctccggc aaccccacct gtatggaatg tacgcatcgt caactgagta
    43981 ccaggttcac cgattgattg agcggcgatc gtaccaactg cttctccgac ttcaacgtca
    44041 gagccagttg caaggttgcg tccgtaacaa tgtttgcaga caccatgttt tgtattacat
    44101 gtgaagacag aacggattgt cactgtttcg acacctgcat caacgatttg ttttgcgatg
    44161 tcttcggaaa tcaattggtt tcctgcaatg atcaattcat ttgtttcagg atgacgtact
    44221 tctttacggg tgtaacgtcc cactaaacga tcttctaatg attcgatgat ttcatttccc
    44281 tcgcggatgg cttggatttc aagacctcgg tcagtaccgc aatcttcttc acggatgatt
    44341 acatcttggg ccacgtcaac cagacgacga gtcaagtaac ctgaatcggc agtcttcaag
    44401 gctgtatcgg tcatcccttt acgagccccg tgagttgaga taaacatttc caagacggat
    44461 agtccttcac ggaagttcga aatgattggc aattccatga ttcgaccatt aggagcggcc
    44521 atcaaaccac gcattccggc aagctgtgta aagttggaaa tgttaccacg agctccagaa
    44581 tcagacatca tgaagattgg atttttcgct tccaaacctt ccatcagttt ttgttggatg
    44641 ctgtcttttg cagcattcca aacagcaatg acacgttcat aacgttcgtc atctgtgatc
    44701 agaccacgac ggaattgttt tgtaatagtt tcaacttgtt tatgggcatt ttcaatgatt
    44761 tcttgttttt cattcagtac actgatgtca gcgatcccta ctgtgatacc ggcatgtgtc
    44821 gaatgtttgt accctaagtc tttcatacgg tcaagcatct tagaagtttc tgttactttg
    44881 aatcgtttga agacttctgc gatgatgttt cctaagtttt tcttcttgaa cggtaatact
    44941 aattcttgct ctttgatatg tgcaggaata tcagtaccag cttcaacaaa gtatttgtcc
    45001 ggtgtttgga cggtcaagtt gaagtctgtt ggttcattca gataagggaa ttcaggaggc
    45061 atgatctcgt tgaagatgat cttaccaact gttgtgatca tcgtacgttc tttttgccat
    45121 tcagtaaatg gtttttcacc taatgagtta gggttcacac cgatacgtga atgtaaatga
    45181 acatatccgt tgcgccatgc gataacggct tcgttttgat ctctgaagac cattccttcg
    45241 ccttcgcgtc cttcttcttc cattgtcaag tagtagttac ctagaaccat atcttgggaa
    45301 ggagtaacaa ctggtttacc atctttaggg ttcaagatgt tttgagcagc taacatcaac
    45361 atacgtgctt ctgcttgtgc ttcttcattc aatggaacgt ggacagccat ttggtctcca
    45421 tcgaaatcgg cattataagc ttcacataca agtggatgca aacggattgc gcgtccttgt
    45481 actaaaactg gttcgaatgc ttggatacca agtcggtgaa gcgtaggtgc ccggttcagc
    45541 aatactgggt gttccttgat tacgtcttct aaaacatccc atacttcgtc ttcttgacgt
    45601 tcgattttac gtttcgcatt tttgatgttt gaagctagtt cgcgttgaac aagctcacgc
    45661 atcacgaatg gtttgaataa ttcaatggcc atttcttttg gaagtccaca ttggtacatc
    45721 ttcaagaaag gtccaacaac gataacggaa cgacctgagt agtcgacacg tttacctagc
    45781 aagttttgac ggaaacgacc ttgtttccct ttcaacatat gagaaagaga tttcaatggg
    45841 cggttacctg gtccagtaac tggacgtccg cgacgaccgt tatcgatcaa tgcatctact
    45901 gcttcttgca gcatacgttt ttcattttga acgatgattc caggtgcatt caagtctaac
    45961 aaacgtttca aacggttatt acggttgatt acacgacggt aaagatcatt caagtcactt
    46021 gttgcaaaac gtccaccttc taattgaacc attgggcgta aatctggtgg aataacaggg
    46081 ataacgtcca taaccatcca gcttggttga tttcctgaag cgcggaatgc ttctaaaata
    46141 tccaaacggc ggattgcacg tgtacgtttt tgtccttgtg cagatttcaa ttcttctttc
    46201 aattcggctg cttctgcatc tagatcaaca ttatcaagta attgtttaat cgcttctgca
    46261 cccatagcag cttggaattc ttgtccatat tgttcacgtt tttcgcgata ttcgcgttct
    46321 gtcaacaatt gttttttctc taaattcgta tttcctggtt cgatgactac atatgaagca
    46381 aaataaatga tttcttccaa agcgcgtggg ctcatgtcta atacaagacc catacgtgaa
    46441 ggaatccctt taaagtacca gatatgtgaa acaggagctg ctaattcaat atgtcccatg
    46501 cgttcacggc gaactttcga acgagtaact tccactccgc atcgatcaca aacgattcct
    46561 ttataacgga tacgtttgta ttttccgcag gcacattccc agtcttttgt aggaccaaaa
    46621 atccgctcac agaacaaccc ttcgcgttca ggttttaagg tacgatagtt aatcgtctca
    46681 ggttttttta cttcaccgta agaccagctt ctgatttttt cgggagaagc caaacctatt
    46741 tgcatacttt cgaatttatt tacatcgatc aaaaggggtt ccctccattt catttaatgg
    46801 caaaccgcaa gacggctgat cttcgaccgc cttgcagctc atagaccgga ttagtctaaa
    46861 ttatcttctg cggtttcaaa gttttggatg acatcgtctt tttcatcttc cacttgttca
    46921 gcagctttct tttctaattc tttggcagtt tgctgttcgg caaattttgt caaagcatca
    46981 acagtgatca aatcgtcgtc ttcatcgtcc atgtcgcgaa gttcgatctc tgtttcttcg
    47041 atatccaaga cacgcatgtc cagacctagt gattgcaatt ctttcaccaa tacgcggaat
    47101 gattccggta cacctggttt tggaattggt tcacccttga cgattgcttc gtatgttttc
    47161 acacgtccga ctacgtcatc tgatttgtat gtcaagattt cttgtaaagt ataagccgca
    47221 ccgtaagctt ccagtgccca tacttccatt tcaccaaaac gttgtccacc aaattgagct
    47281 ttacctccca atggttgttg tgtaaccaat gagtaaggtc cgattgaacg tgcatgcaat
    47341 ttgtcatcga ccatgtgagc aagtttgatc atgtacatca caccgacaga cacacgtcca
    47401 tcaaatggtt cacctgtacg tccgtcgtac agaacagttt tcgcatcgct ggccataccg
    47461 gcttcacgaa ctgtttccca tacgtcttca tcacttgcac catcaaatac tggcgttgct
    47521 acgtggatac ctaactgacg tgcagccata cccaagtgta attccaatac ttgcccgatg
    47581 ttcatacgtg aaggcacccc taaaggattc agcatgatat cgattggtgt tccatcaggt
    47641 aggaatggca tatcttcttc cggcataata cgggatacaa cccctttatt tccgtgacga
    47701 ccggccattt tatccccttc atggatttta cgtttttgaa cgatataaac acgaactaac
    47761 atgtttacgc ctggtgacaa ttcatctcca gcttcacgag taaagatttt cacatcgtga
    47821 acgataccgc cgccgccatg tggtacacgt aatgatgtat cgcggacttc acgagctttt
    47881 tcgccaaaga tcgcatgcaa tagacgttct tctgcagata attctgttac cccttttggc
    47941 gtgactttcc ctacaagcaa atcgccatct tggacttctg caccaatacg gatgataccc
    48001 atttcgtcta aatcttttaa tgcgtcttcc ccgacattag ggatttcgcg tgtgatttct
    48061 tcaggtccta attttgtatc gcgggcttct gattcatatt cttcaatatg gatagaagtg
    48121 tatacgtcat cttttaccaa acgacggctc atgatgatcg catcttcata gttgtaccct
    48181 tcccaagtca tgaaaccaac taaaacgttt tggcctagag ccatttcgcc ttgttccata
    48241 gatggtccgt ctgccagagt atcccctttt tcgactttct cacccaaatg aacgattgga
    48301 cgttggttgt agcttgttcc ggagtttgaa cgacggaatt ttgttacatc gtatttatct
    48361 aatgcaccgt tgtcgcggcg tacgcggatt tcagaagcat caacgtattc aacgacaccg
    48421 tcatgtttac ataacaaagc agctcctgag tcatgcgctg atttgtattc cataccagta
    48481 ccaacccatg gtgcttgagg attgatcaac ggcactgctt gacgctgcat gttggcaccc
    48541 atcaaggcac ggttggagtc atcgttttcc aagaaaggaa tacatgctgt cgcaacggca
    48601 actacttgtt taggtgatac gtccatgtag tctactttat caatagatac ttctaagttt
    48661 tcactttgag cacgagccat aacaacgtct tgagcaaatg ttccatcttc attcaatggg
    48721 ctgtttgctt gggctacgat gtaatggtct tcgatatcag ctgtcaagta gtcgatttgg
    48781 tcagtaacac gacctgtttc acggtctaca cgacgataag gtgtttcgat aaatccgaat
    48841 ttatttactt tcgcataact ggacaaacta ttgatcaacc cgatgtttgg gccttcagga
    48901 gtttcgatcg gacacatacg gccatagtgg gagtagtgaa cgtcacgaac ttcataaccg
    48961 gcgcggtcac gagtcaatcc gccaggtcct aaggctgaaa gacgacgttt gtgtgtcaat
    49021 tcgccaagag ggtttgtttg atccatgaac tgtgacaact gagaagaacc aaagaattct
    49081 ttgatacttg ccacaactgg acggatattg atcaattgtt gaggtgtcaa tgtttcagta
    49141 tcttggatcg acattctttc acgaaccaca cgttccatac gtgctagccc gatacggaat
    49201 tggttttgta aaagttcacc gactgaacgg atacgacgat ttcctaagtg gtcgatatca
    49261 tcgacattgc cgataccttc catcaagttc aagaaatagc tcattgaagc aatgatgtcg
    49321 gctggacgaa ccgtttttac agctgcttct ggatagccgt ttccgatcac gtttacttca
    49381 cgttcagggt ctcttggtga aaatactttg atgacttgga cagtcattgg atctgtgacc
    49441 acaccgtctt ctgatggata gtaagtcact gaatttaggc cattatcaat gaaaggagct
    49501 agtgtttcca tgacttggtg agtcagaact gttccttttt caacgatgat ctcgcctgtt
    49561 tctggatcaa ctaatgtttc agctaatgtc agattcaata gacgtgtttt caaatctaat
    49621 tttttgttta ctttgtaacg accaacgttt gctaagtcgt aacgttttgg atcgaagaaa
    49681 cgagcattca gcaagttacg tgagctgtct gctgtttttg gttcgcctgg acgcaaacgt
    49741 tcgtaaacgt ctttcaaacc ttcttctgtt cttgagtcgc ttgcgttttt atgcaagtct
    49801 ttttcgactg tattgcgtaa tgtttcgctg tcgccgaaga tttcaaagat cgtatcatcg
    49861 gaaccaaatc ctaacgcacg aactaatacg gttaaaggaa tcttacgtgt acggtcgata
    49921 cgtacataag agatgtcttt tgcatcagtt tccatttcta gccaagctcc acggttaggg
    49981 ataacagtag aaccgaatcc ttcttttccg tttttgtcga cttttccatg gaaatagaca
    50041 cctggtgaac ggactaactg agaaacgatt actcgctctg ccccgttgat gatgaatgtt
    50101 ccttgttctg tcattaatgg gaaatcaccg aagaatactt cttgtgcttt gatttcgcca
    50161 gtctcgcggt ttgtcaaacg caatgttaca tgtaatggtg cagagtagtt tgcatcatgc
    50221 gcacgagctt ctgctactgt atactttggt tctttcaatt cataatctac aaattccaaa
    50281 gatagattgc cattgaagtc atcgattggc aaaatgtctt caaacatttc tctaagtccc
    50341 tcatctaaga accattggta agagtctgtt tgaatttcga tcaaatttgg taattccaac
    50401 acttcactga ttcttgcgaa acttctacgt tcgcgatgtt ttccgtattt tactacgtgt
    50461 ccagccaagc tcttcacccc tctaaaatgt cattcaaaaa gactacccga tcaattgtta
    50521 caataatatt tcaaatatta tttttaggta acaacagaca ttttttgcgg cttttaggca
    50581 aaaaaaaacc aaaaatagaa cattctcttg aaaccttact tcgtgagctt ctacttttgt
    50641 ttccttatat taaagccgaa acggacaata tttcgtttct gccttttctt catcagatag
    50701 tttttgcaac ttcatatact acacttattt tctataaaag tcaacacttc ctggcgtttc
    50761 ttctgctttt tcgcaacatt ttcttaatgt tttcaatttt atgttttcaa ttatctgaca
    50821 attcttaatt gacagacctt tttatctcgg ttatacttta gaaaattcat tttgtggagg
    50881 aacttctatg cgaaccaaca tcaaccaaat cgcaccaaca caactgaata acttttactt
    50941 tagctacttt agataggttt gtattcatca tgatggatac aaacgttaaa cgacagtttg
    51001 tatctatgat ggctaaagaa ctttgtattg cagcaaaaag ccacgtagac aaaatgctaa
    51061 gtggctgagc agtgaaaata caaagtaaaa gtatcaagca ttctactttt tcaatttatt
    51121 ttccgacttt tcaatccatt tggacatttt gtctgaatgg attttttgtt tcccaaaatt
    51181 aaggaggaaa aagaaatgaa aaaaaatata atcatcggat taggcgctgc cctgctacta
    51241 gctttaggag gttgcgcaca aaaagacaat tccgcagagg catcagatgg acaaaaaaca
    51301 attggcgtat tgcaacttgt acaacataac tcgttagatg ctgcctataa aggattcaaa
    51361 gaaggattag cagaagaagg gtataaagaa ggcgataatc taacaatcga ttatcaaaat
    51421 gcccaaaaca atcaagacaa cctgaaaagt atgagtgaga aactcgtcaa agaagatcct
    51481 gatttgctac ttggaatcgc cactccagca gcacaatctt tattaaatga aacaaccact
    51541 atcccgatga ctgtcacagc ggtcacagac ttggaacaag cccatttagt cacatccaat
    51601 gaaaaacctg gcggcaatgt gactggcacg acagatatgg tgccaatcga aaaacaaatc
    51661 ggcttactgt taaatatcgt gccagacgcc aaaacaatcg gcatcatgta taacaacggt
    51721 gaagcaaact ctaagatcca agctgatttg gcagaaaaag aattgaaaaa agcgggcgtc
    51781 aaagtcaaag tactgacagc aaattccaca aatgatgtcc aacaagtgac gaaaagtcta
    51841 gctaaagatg tagatggaat ctatattcct actgataaca cgttcgcaag tgcggcaagt
    51901 gttgtcggtg aagtcgcaaa agaaacaaaa acacctgtcg ttgctggcgc agttgatcaa
    51961 gtcaaagacg gcggtctagc aacagtagga atcgactacg aaagcttggg taaacaaaca
    52021 gggatcatgg cagcaaaaat cttagacggc aagaatccaa gtgaacttcc tgtcgaaaca
    52081 gcgaatgatt tggaactgta tgtcaataaa gacatggcca aagcattagg catcaatcca
    52141 gacagcatca aagaagctga ataaaacaca ggaggattct catgacaaat ttgctctctc
    52201 ttttcttatc atctgcatcg caaggggtct tatgggccct acttgcgatt ggtgtatacg
    52261 tcactttccg tatcttggac attgccgact tgactgctga aggaagcttt cctttaggtg
    52321 cagggatcgc ggcagtaagt attacaaatg gctatcatcc attgacagct tgtcttctag
    52381 gctttttagg gggtgcggca gcagggcttg tttccggact gcttcacaca aaattgaaaa
    52441 ttcccgccct tcttgcaggg atcatcacca tgactggttt atattctgtc acatcccgtg
    52501 tgatgggtgc gccgaacatc tctttattgg gtcagccaac gatctttcat tgggtcgagt
    52561 cattcggcat aacaaaagac aacgctgtac tgatcgtcgg tctgcttttt gctttgatcg
    52621 ttgtatgttt acttgttttg ttcttacgta cagaaacagg acttgctatt cgtgcaactg
    52681 gcgataatct ggcaatgagc gaagcaaacg ggatcaacac agataatatg aagatcatcg
    52741 gttatatgat ttctaatgga tgcattgctc tttctggctc cttgcttgcg caaaacaacg
    52801 gctttgctga tttaaattct ggtattggaa cgatcgtaat tggacttgct tctattatca
    52861 tcgcagaagt tttgttacgt aaccagccac tatttctccg tctcttgact gtcgttttag
    52921 gtgcggtcat ttatcgtttt attttagcgt tagtcttcga actgaatgtt gaaccaagtg
    52981 attcgaaact tgcttctgct ttcgtgctag tgatctgtct ttctttcccg cagatccagc
    53041 aaaaattcca tttacctcaa aaaaagaaag gcggtagtgt caaatgaatg acgtcttaac
    53101 attggaaaat ctccatcata cctttgaaaa aggaacgatc aacgaaaatc atgtcctgaa
    53161 aggcattaat ctttccttag atcccggcga cttcgtgacg atcatcggtg gaaatggggc
    53221 tggtaaatct actttgctga acagcatcgc cggtacattt tctgtcgatc agggtcaaat
    53281 tttattgaat ggaaaaaata tcacgaaaaa atctgttgtc gaacgttcga aatccatcag
    53341 tcgcgtattc caagatccta aactagggac agccgttcgt ttaacggtag aagaaaatct
    53401 tgctcttgct atgaaacgag gaaaaaaacg cggcttcttc cgtggtgtca aaccacaaga
    53461 ccgaacattt ttcaaagacc agctggttcg gctaaatttg gggttggaaa atcgtttatc
    53521 ctcagaaatc ggtttgctgt ctggaggaca acgtcaagct attacgttat tgatggccac
    53581 attacagcgt ccagaactga tcttattaga tgaacacact gcagcgcttg accctgctac
    53641 ttccaaaacg gtgatggaat tgacagaaaa gctgatcact gagcagaatc tcactgcttt
    53701 tatggtcact catgacatgg aagacgcgat ccgctatggg agtcgactga tcatgctgca
    53761 tcaaggaagg atcgtcgtag atatccagca agaggaaaaa cagcagctca cagtgcccca
    53821 attattagcg ttgttcaaac aaaacagcgg cacggcatta aaagacgatc aagtactttt
    53881 atcttcataa tcagaaagag gatgggacaa aaaatgaacg cccaccctct tcttttttct
    53941 ctttccttct ttcgtttttg ctttatgtat caaccgttgt ttcttcgaaa actattttgt
    54001 tctctccttc ttcctgatag aatggatggg aaaggagctt gatgccatga caacgaaaga
    54061 caaggtactt caattattga aaaagacacc agattttctt tccggagaaa agttggcaca
    54121 gcaactggaa gtttcccgga caagtatttg gaaagccatc aaagaattgg aaaaggcagg
    54181 ctatcgtttt gagcacgggc ctacagggta tcgttacctg ccctctgacg tactagatgc
    54241 aaaagaaatc cagcaagagc tacggtcgtt tattcctgat ttgaaagtac aaacgatgtt
    54301 gacttctgaa tctacgatga aagatgccaa actggcagca gctcaaggag attctgtttc
    54361 gaagttattt gttgctgata cacaagaaca agctcgtgga cgttttggac ggcctttttt
    54421 cgctgaacct ggacgaggaa tctacatgag tctcttgctc catccagaca aacggtttga
    54481 tgaattgcct caatatacga tcatcgcagc cagtgcctgt gtgaaagcca ttgaatctgt
    54541 aaccggtcgt tctgcttcga tcaaatgggt caatgatatc tacatggatg gaaaaaaagt
    54601 ctgtgggatt ttatctgaag cagtcagtga tatggaaagc ggcaggatca gtcatatcat
    54661 cattgggatc gggcttaatt tttctgttcc tcaaaatcat tttccagctg atctccaaga
    54721 aaaagccatc tctgtctttc ccgacggaca agctagtgtc acaaggaatc agctaatcag
    54781 caaaatctgg caagaattgt tcacttatct ttctcaccca gaaaaagcgt tggacttcta
    54841 taaagaaaaa tcattcgtat tgggaaaaac cgtgtcgttc aatcaaaaag gaacgactta
    54901 tgtcggtctt gcaaaagaaa tcggtgatca tggcgaactg atcgtccaaa caaatgaaca
    54961 gaagatgagt ttgtcttctg gcgaaatcag tctcacatct atccgtacgc aataattatc
    55021 agatttcaca aaaggacacc tgatctcata cagtgtcctt ttttctgaat tctcttcaaa
    55081 agactctcct tattctatca gctgacagtt tctctatcct ctagaaaaag gtattctaaa
    55141 aatatctata aagcatggta cactttcttt aaatgaatca atgcaaaaaa caagagagtc
    55201 atcagtttac acgatcaaaa gagatagcag gccgcttcct ttttcaatct tatagataga
    55261 ccatgggtaa cgataaaaac aaaaaagaac ttgcgactta ttttgccgta agttcttttt
    55321 tagctagtgc cttaatctct taatcccaac aaacgtctcg catagatcac ctgaccagag
    55381 tgcatagcag catcatcaat aatagaaacg agacgcattc ctcgtgtgac tggtggtgtc
    55441 cagttctcgt caattacttc atctagctcg gaaggttcca gccttcttaa gtaagaaata
    55501 gcaaaatcag cagctcctcg caagtatcct agcagatctt ctatttgatt agcttggatc
    55561 tgctttgctt gttccagact atgcttccaa tcttgttcgt tatctgctat cgtcatcgga
    55621 aaccggcttt cccagtcttg actatgccaa attggagaag ttcctgctaa atcagcaatc
    55681 tggaaatcta attctcgagc ggtatgccaa gccagccaag tcattgattt gatctgcggg
    55741 gctctatctt ccactggaaa acgattggcc tcctcaatag agacgccttc aaacaaacgt
    55801 tcaaaccgat cgtatgcccg atccacactc tcgcatagca tatctaaata atccatttat
    55861 tttcctcctt tttatttatc ctacttactt tttcctaatt ctaggatacc tacttatatg
    55921 ttactacaca cccttatttt ttacacttta acagtcacca tcatcaatag tttggagatt
    55981 gaaagaaaaa gatagtgtcg aactatcaat tgaacaagaa gcaacaatat gaacgtttca
    56041 ttaatattct ttaaataaac aatgattttt caaacgaaca gtttaagact aggagaaaaa
    56101 gagtataata ggaaaatgtt tgaaaggaaa ggaaggggaa aaatgaaaaa aagggcaagt
    56161 ttactagtcc tagttgcttt gttgttcaat atcttttcgt atggtgtggc cgtttcagcg
    56221 gaaacataca ttcctaaaga ccctgggaat atttataatg atctaactgg taactatggc
    56281 ttgttaggaa ttgctagtca attccacgta tttgctaaaa ataagacaac tgtgaatgca
    56341 catacagacg gaaatatagc aacacatgaa cttgatgcaa aaaataattt tggtacagag
    56401 cttcatgaag gtttattgaa ccaagaaata aattatatac aaaaaactga ttctttcgct
    56461 aattcttcag gcatacctac aggtagcaca agaatgaata aatttgtcgt tggcagtaac
    56521 gtggatgtag cacttcaaga tggtcatcct acagtcaatg gatccaggct tgatcactta
    56581 actccagatg atttttataa agatcgtgct gggcatgaat atattgattt cgagcaggaa
    56641 tttgctaaat tgaatgtagc agcaaatgat cttgcaacga tcactccagc taaaacatat
    56701 actgctgcag attttccaga catgaacaat cggacaatcg atttaactga tttatcagat
    56761 accgggtttt tattggtaaa tatcgatgca gaagtgttaa cgatgaatac gccacttcaa
    56821 atcattaatc caaatgatca agtagttgta tttaatgtca tcaacagttc ttctgcattg
    56881 aacgtacagt caccaatcaa atacaatgat cgttcaaatc atgaaacaga agatttttcg
    56941 gatgcgaata tctcttggaa ttttggaaat gagatgactg atttgacaat cagtgctcct
    57001 tttcaaggaa caatccttgc gcctaatgct actatccgca ttactcaaaa ccaagacgga
    57061 acgattatag gaacaaatgt gattttagat gcagctacca atcgttggga tcctaacgaa
    57121 atatttatca gcgacaatgg tacagatacg acagacacaa gcgacagtac agatacatct
    57181 accacaagtg atagctcaga ttcatcaact tcaagcgaca gtacagatac tacctctacc
    57241 acaagtgata gcacggatac atcagcttca agcgacagta cagatactac ctctaccaca
    57301 agtgatagca cggacacatc agcttcaagt gacagtacag atactacctc taccacaagt
    57361 gacagcacgg acacgtcagc ttcaagcgac agtacagata ctacctctac cacaagtgat
    57421 agcacggaca catcagcttc aagtgacagt acagatacta cctctaccac aagtgatagc
    57481 acggacacgt cagcttcaag cgacagtacg gatacatcta ccacaacaag tgatagcacg
    57541 gacacgtcag cttcaagtga cagtacagat actacctcta ccacaagtga tagctcagat
    57601 tcatctacca ctacaagtga tagcacggac acgtcagctt caagtgacag tacagataca
    57661 tcctctacca caagtgatag ctcagattca tcaacttcaa gcgatagtac agacacgtcg
    57721 tctaccacaa gcgatacacg tcatgcaagt gtctccttct caaaaacaag ttcggataac
    57781 aggacgaact ttaacttaac taataaaaca agcaatgaca atggaaacaa atcaaaaaga
    57841 gcacttccta aaactggaag tcaatcgaat aattggataa cactcgcagg cgttatatta
    57901 cttgtaatcg gtttaagaat cacttttatg agttataaga aatcgaaaag gtaaaaaaat
    57961 attcaagata aaaaagagca acgtcgctaa cggacgttgc tctttatttt tgctttttat
    58021 cataaataca agaactacaa ttcaatttgt caaaaaacac gtagatttcc ttctgagaaa
    58081 tttatttacg tatcgaacct ttccctattc agtctttgcg atccaaaatc agttgggtaa
    58141 gggaaaagag attctctttt gttcgaagag aggtggcagg taatttcttt atttccttca
    58201 aagccttttc tgtgtaagag gccgctaact tcctagcttc ttccacgcct cctgtatgtt
    58261 caacgatttt gcgtacggcg tcgacatctt cttctgtcat tgccgctttc ttctctaaat
    58321 aaggtagtag ctttggtttg tttttttgca aagagtagat caacggtaaa gaatagaccc
    58381 cttgacgcat gtcttctagt acaggtttgc cgatttcctg gctctctttt gtataatcta
    58441 aaatatcatc gataatctga aaggccatcc cgatagaaag tccgatatct cctgcttttt
    58501 ttgcgaagcg ctcgcttgtt ccactttcaa aagcgccaac agaacaactt aattggaaca
    58561 gttcagctgt cttacctgaa atattttcca aatattcttt tacagaaagt tcataattat
    58621 agcgatcatc catctgccct aattcgccat tcaatacttt ttccatactg cgagaattca
    58681 attggagact tttcaaggaa gtggagtaat cagacaatag tttaaaacag cagacaaata
    58741 aatagtctcc tgcatagaca gaaacgacat taccaaactg actacggata ttaggctgac
    58801 cacgtcgcag atccgcttca tcaacgatat catcatggat cagtgtggcc gtatggagca
    58861 tttcgataga agctgccaaa gcgatggctt tgtctcgatc ttgctctggt ccaaattgtg
    58921 agaacaatag ctgataggca ggacgcaaca gctttccgcc ggcatggatc gtttctttga
    58981 ttgcttgttc gacaggtttg tttttcaggc ggacagtttc ttccatccgt tttagtgttt
    59041 ctgtcaattc tttacttaaa gcaggatagt tttcccacat tggatgtagc ttcataaaaa
    59101 aactccttgt gtgacgatta ttttttggta aacattgggc tccatttttg tttggttccc
    59161 tgctcataat ctttttggaa tcgttgaagt gtctgcacat gtcgtaaaag ataattcgct
    59221 gcgatcccga ttgcaatccc tgataaaatc cctaaaactg aaaggactgg caagtacaac
    59281 atgactgtcc atgattgtgc aatgaaagaa gccatcaaaa gctgtccgac attatgcatg
    59341 aaaccgccgg ccgcactaat tccgatgata ctgacacgtt ttggtcctaa ttgcttgacc
    59401 aataacattc caaaataact aagcaaagca ccactcatac tataaaggaa tgtggaaatc
    59461 gttccgccta gcaaagtagt caggaatagt cgtagccaaa gtagtaagaa gctgtctcgt
    59521 tttttcatgg taaaaatggc aacgatcgtg attagattgg ctaagcctag ttttgcacca
    59581 ggcgcaaaag aaaaaggata agggatcata ttttccaata aaccaatgat cactccttgt
    59641 gcaacaagta gcgaaatata aatcattttt tgtaatttac tcatggtgtg tgaaggtcta
    59701 ataaatcaga ctgtcttcgt cacctccatc tgttgaacgg acttcgatca ctaatttatg
    59761 tggcaaacag acgatggttt cgccattttt cgttgcccag cctcgacgta cgcagatctg
    59821 gtcatcgcaa tctgcttctt ttatccgtac tcgatcgcca tctatctcga tcaagttata
    59881 atctccgtgt tcatctttgt aagtatatgt gttgctctct tctccagcca tcaaagggaa
    59941 tgttttgatt tctgtgccat ccacacgcag taccgcttct ttttctgctg ttgcggtgtt
    60001 ttgctgaaga ccaaaaatca tgactggtaa aaaagaaaga acagtcaaaa gcaaaataat
    60061 aaaaacatcc caaggtttga gcagactttt ttcagcaaat tctttaaggc tcatttttgt
    60121 tctccttttt ccagattaac tcacccaatt ttagcataaa aacaagaggt tgtgacacct
    60181 gtgtcttgct ttgagtaata agaacgaaga atagaaaaca gctttccatg ttttctaatt
    60241 ctttgggctt attaccgaga agcagcaggt ggaacaccgt ttataacaag aggttgtgac
    60301 agaagtgggc aacttcaaga aataagaagg aatttacgaa aattactttt caagtttttg
    60361 tgaaatttcg gcttattttt cgaagaagct acttctggaa caccgtttat aacaagaggt
    60421 tgtaacagag gttgtgacac ctgtgtcttg ctcatggagg cttatctcgg gcatcttatt
    60481 cctttaattt tacataaaaa ggaggctgtg acggtttgtc tcagcccctt tctattgaaa
    60541 tcttgcattc aaataacgtt ctttatctat ttatttcttg ccgtactggg aacttggtct
    60601 accgtaccac caattaccta gtgtacgttg tagccatgga attacccagc gatccaaccc
    60661 aatcgcacgt ccggaaccgt tcatcaatgc gaaagcaaca aaaatgaacc aaatgtttac
    60721 ccagtaaaac atgcctgata agcagaacgt gatcaccaat acaatcgttg tcgcactgca
    60781 tagccatgtg aataatccag caatcaatgc taatgccaag gcaatttcaa agaataccat
    60841 gaatttttgg aagaacattg ctacatcatt gtttggcatg atcacttgca tgatctttgt
    60901 gaaccagtct ggtgacttct caatcacagc cattggttct tcaccgtatg cataactcaa
    60961 accaaaatgt gcagcttgag tcgctgcttc tccggcactt gcccctgctc cgctggcagc
    61021 tgtggttgca tcaccagcag cagaagcacc actggcaaca tctgttacat tgtattccca
    61081 tgtccaaggg aacacactgt ttccagtgaa ccatgatcct tcaccaaacc aagtacttgg
    61141 tttgaacact tcaccattcc caatgatttt tttggaagct tcaacgaacc aaactaaacc
    61201 atagaaaata cgaagtggca cactccataa gacattgccg taacgagaag aatgtcctcg
    61261 agtcacgttg cgatcatctt tgatatggaa aaactcatgc attaaatatt ggaacatata
    61321 ataaccagaa cgaatatcaa agaagtattt caaattcacg atatgtttca ttacgatagc
    61381 taaaaatccg cttaaatgga tcttatcaaa taggttagct accccccatt tggcaccaat
    61441 agaaaccata aatccttgat agtttccttt aaatttatgc ttttctccgc ctttgatcga
    61501 agccacgata ttcgctgcgg ctgtgtgtcc tgtttgttca gcagcttgaa cgatctgcgg
    61561 tgtcggtgtt cctgggaatt cttcataaaa gactaaatca ccaatgatat agatgttttt
    61621 atcttcgtag ccttttgcct gcatatattc attggcgatc aaccgctgtc cgcgtgctga
    61681 ttctaatcca aagtcagcag cgtcagaagt tcctttgaca ccagctgtcc aaatcagtgt
    61741 gtgagtcgga atgttgcgtc cgtctttcaa tttgatatga tcagcagcca cttctacgat
    61801 tggtgcattc aataataatt ccacgttgtg cttacgcaaa tagcgttcag cttttgcagc
    61861 atcatttcga tccaacatat tcaagatcgt cggcattgcc tctacgacca tcagtgtgat
    61921 ttcgtttgga tctaatttat aatctttggc taggcgattt ttccagtcga tcagttcccc
    61981 aatcatttca ataccggtaa atccagaacc acagacaacg aaagtcagca tggctttacg
    62041 cagttcatca tctggttcca atgcagcttt tgctactgtc ttctcaatat gttcccgaat
    62101 ccttacagaa tcttcaaatg accaaagggt gaatccgtgt gctttgacac caggtgtacc
    62161 aaagtcattc ggctctccgc ccattccaag gatcagctga tcaaaaggat aggaacctaa
    62221 tttggtcttg acgatttttc gttctttatc gataccgatt acagtgtctg tgacaagttg
    62281 tacattcttt tgacgagcaa acaaatgctg cagatcgtat tggattgctt ctggttctac
    62341 acgtccgcca gctacttcat gcaactcggt catcatagta tggtacgaat gacgatcgat
    62401 cagagtaatt gctacatctt cttttttcag tttctttgcc aagtattttg tagctgaaac
    62461 accggcatat cctgctccta caacgacaat attttgcttt gccattgatc atccgctcct
    62521 tatagactat ctatataatg aatatatgat aattctttta gtttgatact tattacacaa
    62581 tctccatata ttatatagat gttctgttct tttgtctaac tccttttgga tttttttcaa
    62641 attttaaaat aaagcgtttt ttcacgaaat ttgctgaaag aaaacgtttg aaatattaca
    62701 tgggttattt atcaaaaata gttgacttat aggctaaaaa gattagaatg tataatgaat
    62761 cacaaagaaa aattgtggac tagaaatgga atggtggggt attaaaatga agaaaaaacg
    62821 ttttgtaact ggatttgccg tattagcatt ttcagctcta gtgttaggcg cttgcggcgc
    62881 agatgacaac ggaagctcaa actcttcttc tgaatcatct acagcacaat cttcgactgc
    62941 aaaaagcaca acagaatcat ctgcaaaagt tgtagctggc ggagatttgc aagacggcac
    63001 atataaatta gaagaaaaaa attattcaaa tggttaccgt gcagtctttg aaatggttgt
    63061 aaaagacggc aaaatcactg aatcaaaata tgataacgtc aatgaaaacg gcgaatcaaa
    63121 aacaaaagat gcagaataca acaaaaacat ggaagcaaaa tctggcacaa acccagaaaa
    63181 atacattcct gagttgaatg accaattctt aaaagcacaa agcgcaagcg gtgtggaagt
    63241 cgtaactgga gctactcact catctgaaag cttccaaaac tacgcacaac aattgatcca
    63301 agctgctcaa gctggtaaca cagatacaat cgaaatcgac aatggtgctg atttgaaaga
    63361 cggcacttac aagcttgaag aaaagaacta ctctaacggc taccgtgtgc aattcgaaat
    63421 gacagttgct ggcggcaaag taactgaatc aaactttgat tatatcgaca aagacggtaa
    63481 atcaaaacaa gatgatacag aatataatga aaatatgaaa gcaaaaagcg gtacagaacc
    63541 aaaaacttac atccctactc tgaacgacga attcgtcaaa gcaatgggcg aagaagatgg
    63601 ctcacctgct aatgtagaag ttgttacagg tgctacacac agctcacatt cattcatcat
    63661 gtacgcacaa caattagtga atgcagctga aaaaggcgac actcaaacaa tcgaagtaga
    63721 taatatcgta acagaaaaat aatcacataa tcatactaat aaaagttgga gggtgggacg
    63781 atagccagat taggcagtcg tctcaccttt caaaatgaag agaggaaaaa gatgcggaaa
    63841 aaactactat tttccttaat cacactgggc atctctttta tggtgctaac tggctgcagc
    63901 aactcagcaa ataaaacaac cactgaaagt tcagcagcta ccaagatatt gaaagatcca
    63961 tattctgatg aacagttctt gttagggact tacgttcgta tccgtgtgta tgatgaaggc
    64021 aaagaatcag cattgaaacc tgccttcgat cgagtaaaag aacttggtga taaaatcacg
    64081 atcaatcaaa aaggttctga gatcgatgaa gtgaatgagc aagctgggat caaaccagta
    64141 aaagtttctg atgacgtcta cacgttagtc aagcgggcct acgaatatag ccaagattct
    64201 caaggcggat tcgatatggc gattggtgca atcacacaat tatggcgaat cggttttgat
    64261 gacgccagaa aaccttccca agaagaaatc gatcaggcat tgaaattagt ggattatcat
    64321 aagatcgaac taaatgacaa agaaaaaact gtttatctaa aagaaaaagg aatgatcatc
    64381 gatttaggtg caatcgccaa aggttacatt acggacgaag tagtgaaagt cttgaaaaaa
    64441 caaggagtga ccactgccat cgttgacttg ggcggtaatg tctatgtctt aggccacagc
    64501 ccaagaggcg aaaaccaaga ctggacagtc ggcatccaag atccgaacat ggctcgcgga
    64561 tctgttttag gttctattaa agaacggaac aaaacattag ttacctctgg aatttacgag
    64621 cgttatttag aagtagatgg caaaaaatac catcatttgt ttgatccaaa aacgggttat
    64681 ccgttcgaca atgatatcgc aagtgtcacg atcataacag ataaatcaat tgatggtgac
    64741 gggttgtcta cagcagtctt ttcaatgggt gtgaaaaaag gactggaata cgtcgaaagc
    64801 gaattgaata acgggacgga agcgatcttc gtgacaaaag acgataaagt atatgtgacc
    64861 gatccaatca aagacacctt caaattatcg gaggactccc attacacgat gggtgaccgt
    64921 tctgaactaa aataaggagt acgaaccatg acactaaaaa tctttttaga agtagttgaa
    64981 atacgaacaa aggtggctag tgtatttcct tttgcggtag gggtattatt ctctatcgct
    65041 ttttttcatg agatacattg gctaaacact tttttatttt ttattggaat gctagttttt
    65101 gatttagcga ctaccgctat caataattac atggattatg aaaaggctca ttctgaagta
    65161 tataagtatg aagaaaatgt cattggccgc agaaatctat ctcctgtatt gattcgaaac
    65221 atgattttag ctatgattgc atttgttttt gttatcggcg ttattctaac gatcaatacg
    65281 ggttggcttt tacttttgat gggatttatc tgttgtttta ttggagtatt ctatacattc
    65341 ggaccgattc ctttatctcg aatgccttta ggggaaattt tcagtggggt gactatgggc
    65401 cttggaattt ttgccatggt cgtttatgtc aatacattcg gttcagatgt ctatgatctg
    65461 acagtcaact tttcatctgg acaatttgct ttggtaggaa atctttgggc tatagcagca
    65521 atcgtattag cttccttacc cttggtgttt acgattgcca atatcatgtt ggctaataat
    65581 ttgcgggatc ttgaccgtga tattgaaaat catcgctata cactagtttt ttatatcgga
    65641 cgtcctgcgg gaacagtctt attccagcta ttgatgtatg cttgctattt cgtgatttta
    65701 atggggttgt tgagcggtat ctatgaatgg ccgatcttgg tcaccttctt gaccttgcca
    65761 attgtgaaga agcatttgga tcaattcaag caaacgttac cccagcctat cagtttcagc
    65821 tattccatca aaaatatggt cttattcaat gccagttatg tattggggct attactaaca
    65881 atcattatgc aagccattta acagcatgaa gaggttgtga aaaaactgcc ctgcatcaag
    65941 taataagaac agccaaataa aaatagctcc ttatattttt cgtttggttg ggcttattac
    66001 cgcagatgca agcttttgaa caccgtttat caggaggttg tgaaaaagtc gcttagtttc
    66061 gagtattaag aaaaattttg tgaaaatggc tttccacatt tttgcataaa tttggcttaa
    66121 taccgagaaa ctggcttttg aacaccgttt atcagaggtt gtaaaatcag tgttttgtcc
    66181 tgagtattag agaaacaaat ggaaaaatag ttcctcatat tttgccgttt tttgagctaa
    66241 taccgaagga cagctgattg aacactattt gttcggaaaa agtgactaac tatgacacaa
    66301 tttttttcac agtccctttt ttcacttgat ttgattcgta ccagcgaact tgacgagaac
    66361 tttcttggtt tggtaactat atggtattct tttaacatcc tcgtttgctt cttctaattt
    66421 atttatacaa agtcacactt ttttaaacat ctgttcttcg atacatttct atttctttta
    66481 aacatcgata ccaatatttt gaacttaaga gtgcatacca aatggattca tttactcttc
    66541 gtcagttacg aagataatca aaatctccaa cttccctact tatttcgttt tgagaattat
    66601 cgaagcaatc ttacataaat aactaaaaaa caaaaaacca gaaaatcagt atactgattt
    66661 tctggtttta catggtttgt tcaataaaaa gctgttggaa ctgttaatat taaattatcg
    66721 catcgttacg aattcttccg ctcctgttgg atggatggct actgtattgt caaagtcttc
    66781 tttagttgca cccatcttga tagccacggc aaaaccttgc agcatctcat cgacaccaac
    66841 accaatagca tgtaagccga cgattctttc ttcttttccg acacagatca atttcatttc
    66901 gcatttttgg cgataatcat ttaatgcaaa gtacattggg gtaaagcgtg aacgataaac
    66961 tttcaattca ttttcgccat atttttccaa tgcttcttct tctgtcaaac caattgttgc
    67021 aattggtgga tgcgtaaaga cgactgtcgg aacaagttta tagtcaagat aagaatcttt
    67081 ctttccatta aataaacgtt ctgataaacg tctaccagct gcgattgcaa caggagtcag
    67141 atctaattta ccgatcacat caccaacagc aaaaatatgt tcatccgttg tattttggta
    67201 tttatctact ttgataaacc cttttttgtc caattccaca tttgtatttt ccagacctaa
    67261 tttttcggta ttcggtacac gtccaccggc aaaaagtaca caatcaccag ttaaggtttc
    67321 accattttca aatagcaccg tatattcgtc attgttttta gtaatttctt tagcaatatg
    67381 atgtgcgtaa gtttttattc cagcttcttg gtacatttgg atcaaattgt ccgaaagcat
    67441 tttatcaaac gttctaagtg gtcgttcttt tcgatatgcc cacatgactt cactacctaa
    67501 accacgaaat acacctgaaa gttcagccgc aatgtatcca gcacctaaaa cgattgctct
    67561 cttaggtaac tctgttaatt caaaaaatcc atttgaatca attgcgtatt ctccaccagg
    67621 aatggtcatt ttacttgcac ggcctccagt agcgatcaag attttaggag cacgataggt
    67681 ttcaccgttt acttcgatcg tttgactatc gataaaagta gcgtaacctt ttatagcctc
    67741 gacatgattg ctatctaatc ctctttgata agcaccgtgt agaaaatcaa tatatttttc
    67801 tcgtctttct actagaccgg cgaaatcaac attttttaaa tctgcttgga taccgtaact
    67861 tgaggcatct cgttcaattg tttccaaaat cgtacttgct tgccacataa cttttttagg
    67921 tacacaaccg acgttgacac atgttccgcc caattcattc gcttcaatca gcaaaacttt
    67981 tgcgccatgc atccctgctc tgtttgctga agcaataccg ccagagccgc caccaattac
    68041 gatgtaatca tatatttgca ttttgttcct ccttattgaa gagatcaatc aaaaccttta
    68101 tttaacgatc ctttttctac agaaaacaag tgtaacactt ttcttgaatt ttgtatgcga
    68161 agatgcttaa aacagggata tatcagcttt ttccttattt acgttttcgg atcatatctc
    68221 caggtttcac cggctgtgcg actggcatag ttaaaatcat cattggattt ggtgctcggt
    68281 caatggattc gccttcttca ttatgcataa cttcaacggt ttgactgaaa tgactgaatc
    68341 ctggaccata aaattcgatt tcatctccta cagaaaacaa gtttctctga cgtatcgtag
    68401 caatctgtgt tgcctcatca taagataata cttcaccgac aaatttatat actgggattt
    68461 tacgacgttc accgaataat tgttcgtttt cagttggtgt attgtagtaa aaacctgtag
    68521 ctaattctcg ttgtgctact ttccataact cgtcgatcca ttcttgcttg cagacatagt
    68581 tttcaggatc ttccatataa ctgtctacag ccgctttata gacatttgac accgtagata
    68641 cataatggat cgatttcatt cttccttcga tcttgaagct gtctacgccg ttttcaatca
    68701 attctggaat atgttcgatc attgacatat ccactgcact catcgagaat tcttcttcca
    68761 cttcgcctgt atcagttaaa ctgtttcgtt cttgaccaaa aggcatatcg aacaatccat
    68821 acttccagcg acaagattga gaacatccgc cacggtttgc atcccgcatc gacatatggt
    68881 tggacaatgt gcatcgacca gaataagaga tacacatggc accatggata aatgcttcga
    68941 tttcggtatc cgtattttta cgaatctctg ccacttcctc cattgaaact tcacgagcta
    69001 aaacgacacg ttccaaccct tcttctttcc agaattccaa tgtctcaaaa ttagttgctg
    69061 aagcttgagt ggataaatgg atcggcagac ctggggcttc tgtcgcacaa atttcgatca
    69121 aagctggatc agaaacaatc actgcagaaa taccgacatc acgcaaactg cggaaaaatt
    69181 cccctgctcc atcttcattt ccttcatggg tgaccatatt agcagcaacg tataccttcg
    69241 catcatgttc ttttgcaaaa gcaactcctt cagccatttc ttcataagta aaattacctg
    69301 cacggcttct taaaccatat gcattcccgc caatatatac tgcatctgct ccataatgga
    69361 tcgcggtttt taacttttcc agtgttcctg ctggcgctag gatttctggt cgtttcaatt
    69421 ttctttttac tgtcatttct ttcatcatac tccttatttg atagcagaag gatcaatata
    69481 aaagaatcct gtatctaatt cacggttttc aggatgcaat ttactgattt cgctagtcat
    69541 tttttccgct ttttcttgat cccagctacc ttctaaaatt tgtttttttg cttcggcgaa
    69601 tagtcgagtg atgtttacga atttttcgcc tggagtaaat aatccgtcca atttccatgt
    69661 acagtaatga tgctgataaa gttcattcaa ttcattcgag agattcagat cattgctggc
    69721 aaaaatatgt gtgccatgac tatcttcata aatagaataa tgtgtctctt cttttttagg
    69781 ttccgagata aaaagacctc tttcacgatc tttttgttcg tcttgtttcg tatagttata
    69841 gtaattctgt agcagtggac gtttggattg atggatgcat gtagcaccat acacaagggt
    69901 ttctacagga atatcgagtt tttcttccat cgccttcatc tcttcaaaag gaacttcacg
    69961 agcaagtact gctccaactg cgcctttttt cgcccagaaa ttgatttgac gtgcgctcgt
    70021 tactaatgtt tcagcatcat aaatatacgg tatacgcaat tcaggatttt ttttcatcgt
    70081 ataaataata cctgtatcac caactgtgat cacatctacg tttatttctt ttaaaaaagc
    70141 caaatattca ggtacatttt tcattttttc aggatgaatg atcccattta cagcaatatt
    70201 cgctttttta ccatacttgt gtgctaattc taccaattgt cgctgttctt ctcgtgtaaa
    70261 tgaagcaggc agacgcaatc caaattgctc ttcaccgaaa tatagtgtat ctgcacctgc
    70321 agcaagcaac tgttctgctt gttcaatagt ttctactgtg gttatgagtt cgatcataat
    70381 tttctcctcc tacaacttca taatagtagc atagctctaa aaactttcca atacttcttc
    70441 tatttaaagt cggatttttg aaaattattt cacaaaaaat caaaataaag ttcgatatat
    70501 tctgtccaaa tataaaaaga gattgtgaca aaaatcttgt cacaacctct taatcaagtc
    70561 ttactattat aaaaattcag ctacacaggc caagtaacta tagggaacct actgtttcct
    70621 ttttaggtcc gcgaacattc aatgtgatct taccttttga agcacctact gttacgtggt
    70681 cacctaaacg aatttctccg ccaagcaaag cctcgctcaa tcgatcttca atttcttttt
    70741 gaagtgcgcg acggattgga cgtgcaccat attcaggatc aaatccagct ttcccgatca
    70801 catcgattgc agatggtgtg attttcactt gaatatcttg ttctttcaat cgtttgataa
    70861 tcgctttgct catgatttgg acgattgtat ggatttcatc tttactcaat gaatggaaga
    70921 cgacagtctc atcgattcga ttcaagaatt caggtcggaa agctttcttt aactcttcta
    70981 agatacgttt ttgcatggct ttgtgatctt tagtaacatc ttggacattg aatccgacat
    71041 ttttttcttc tcgaatttgt gtcgctccga tatttgaagt cataatcatg attgtatttc
    71101 taaagtccac ttttctacct ttagaatctg ttaaatgacc atcatccaat acttgcagaa
    71161 gtaaattgaa aacgtctgga tgtgctttct ccacttcatc taagagaatc actgaatatg
    71221 gtttttgacg aattttttct gtcaactgtc caccttcatc ataccctacg tatcctggag
    71281 gagacccgat taatcggctt gtactgtatt tttccataaa ttctgacata tcaacacgga
    71341 tcagggcatc ttcacttcca aacatgactt ctgaaagtgc tttggccaat tctgtttttc
    71401 cgacaccagt tggtccaagg aacatgaatg aaccaattgg acgatcagga tctttcaatc
    71461 cgcttcgtgc acggcggatc gcacgtgaaa ctgctttgac agcctcatct tgaccaacga
    71521 cacgttcatg cagaagtcct tccagttcta acaagcgttc gctttctttt ttctccaact
    71581 gctgtagagg gacaccagtc cactccgata caactgtagc aacatcttct tctgttacac
    71641 gatcagaata accttgctcc tgttttgctt caattgcaga aactttttgc agctttttac
    71701 gtagtgcttt ttcttgtctg cgtaattgag cagcattttc aaagtcttgt ttttggatcg
    71761 ctgcttcctt ttcttgtact agttcatcga tttctagttt gatcacagtt gattttgtta
    71821 agtgatcggt ttgatccaaa cggactttcg ctgctgattc atcgatcaaa tcaattgctt
    71881 tatcgggtag ttgacgagaa gtgatataac gaacagaaag attgactgct gctcgtacag
    71941 cttcatctgt gatctccact ccatggtgct cttcatatct tgtacgtaat ccttgaagaa
    72001 tcacttctgc ttcttcagga gttggctcat ctacttgaat gcgagcaaaa cgtcgctcta
    72061 atgcagaatc tttttcgata tatttttgat attcatctag ggtcgttgct ccgatcgttt
    72121 gtaattctcc tcgagctaac gccggtttca agatattcga agcatcaatt gctccttctg
    72181 ctccacctgc accaattaag gtatgcaatt catcaatgaa taagattact tgaccatcat
    72241 tatagatttc gtcgatgacc tttttcattc gatcttcaaa ttcaccgcga tatttcgtac
    72301 cagcaaccaa agatcccata tcaagcatca tcaggcgctt ttcttgcatg tcttctggca
    72361 cttcacctaa aatgattttt tgagctaacc cttcagcaat ggctgttttg cctacaccag
    72421 gttcacccac cagtacaggg ttattctttg tccgtcggct aagaatctgg ataagccgtt
    72481 tcacttctct cgaacgacca acaactgggt ccagcttgtt ttcttttgct aatttcgtca
    72541 aatcacgtgc taatgaatct agcgtaggtg ttccttgcgg tgtttgccgt ttatcttgta
    72601 catttacatt tctacgacga ttcattcctt ttccagcaga atcaccagct agccccattt
    72661 tttgtttcag aacttgacgg gcttttgaaa ggctgatacc taagttgatc atgattcgag
    72721 tagccatgat actttcatct tttaataagc tcatcaaaat atgttctgtt ccaacttttt
    72781 gagaaccagt tttcttcact tcgctgctag ctaaagcaaa aacttgtttc attcttggtg
    72841 aatatggaag aaagactcct ttagggtaag aacggatacc accataacca atcagatgtt
    72901 caatttcttc ataaatttcc gtttcattca aatccatttc acgtaatact tttccagcaa
    72961 ttccattttg ctcgaccact aaggctagta aaacatgttc tgaactaact gattgatgtt
    73021 tcaaccgttt tgcttcttct tgtgcaattt ctaatactgc ttgggctctt tcagtaaaaa
    73081 gttcttccat ctaatctcac ttcccatctt cataacttaa acgttccaga aatgctcgga
    73141 gaatccgagc acgtgtatga tcttcatatt cattgaaatt caatgtgttt tttgcaattg
    73201 cactcaacat caagtttcct tctttttttg taattatttg gtcttcataa agtttttgaa
    73261 taatagcaaa agcatttttc tcactgattg tatcatcaaa taactgattt atctgctcaa
    73321 gtaattgttt tttatctgaa atccttactt ttgcaattcg tatataacca ccaccaccac
    73381 gtttgctttc tactagataa ccacgttgga tggtaaaacg ggtattaatc acatagttga
    73441 tttgtgaagg aacacagtta aagaggtctg ccatttcaat ccttcgaatc tcgatcattt
    73501 cactattttc cagaattttc tttatatatg attcaattaa atccgaggta ttctgatgtg
    73561 ccatcttatt cattcccttt gactaatatt gaccttaatt ataacgaatt tgataataga
    73621 aagaaagaag aacgcttttt gttaaataaa tcctttagaa tcaacaaaaa aaaatacgga
    73681 atatactatt ccgtatcgac gcgcccaaga ggagtcgaac ccctaacctt ttgatccgta
    73741 gtcaaacact ctatccagtt gagctatggg cgcatattca atttaaaaaa attataaatc
    73801 atttgaaaac aaaaagtaaa aaaattataa atcatttgaa aacaaaaagc aagataaaaa
    73861 aaatcgggaa gacaggattc gaacctgcga ccccttggtc ccaaaccaag tgctctacca
    73921 agctgagcta cttcccgttt aaaaaataaa aaagac
//
LOCUS       DAMYXM010000006        68126 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.006, whole genome shotgun sequence.
ACCESSION   DAMYXM010000006 DAMYXM010000000
VERSION     DAMYXM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.006"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(551..2164)
                     /locus_tag="PNS21_000490"
     CDS             complement(551..2164)
                     /locus_tag="PNS21_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDI5158225.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2305..3213)
                     /locus_tag="PNS21_000491"
     CDS             complement(2305..3213)
                     /locus_tag="PNS21_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI5158226.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3396..4520
                     /locus_tag="PNS21_000492"
     CDS             3396..4520
                     /locus_tag="PNS21_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDI5158227.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4747..5664
                     /locus_tag="PNS21_000493"
     CDS             4747..5664
                     /locus_tag="PNS21_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDI5158228.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5657..6490
                     /locus_tag="PNS21_000494"
     CDS             5657..6490
                     /locus_tag="PNS21_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDI5158229.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6573..7691
                     /locus_tag="PNS21_000495"
     CDS             6573..7691
                     /locus_tag="PNS21_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDI5158230.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7685..9583
                     /locus_tag="PNS21_000496"
     CDS             7685..9583
                     /locus_tag="PNS21_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HDI5158231.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9577..10800
                     /locus_tag="PNS21_000497"
     CDS             9577..10800
                     /locus_tag="PNS21_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDI5158232.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10886..12820)
                     /locus_tag="PNS21_000498"
     CDS             complement(10886..12820)
                     /locus_tag="PNS21_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDI5158233.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13046..13702
                     /locus_tag="PNS21_000499"
     CDS             13046..13702
                     /locus_tag="PNS21_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDI5158234.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13776..14129)
                     /locus_tag="PNS21_000500"
     CDS             complement(13776..14129)
                     /locus_tag="PNS21_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDI5158235.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14321..15250
                     /locus_tag="PNS21_000501"
     CDS             14321..15250
                     /locus_tag="PNS21_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDI5158236.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15261..16097
                     /locus_tag="PNS21_000502"
     CDS             15261..16097
                     /locus_tag="PNS21_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HDI5158237.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16345..17121
                     /locus_tag="PNS21_000503"
     CDS             16345..17121
                     /locus_tag="PNS21_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI5158238.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17297..18040
                     /locus_tag="PNS21_000504"
     CDS             17297..18040
                     /locus_tag="PNS21_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5158239.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18033..19613
                     /locus_tag="PNS21_000505"
     CDS             18033..19613
                     /locus_tag="PNS21_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HDI5158240.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19864..20343)
                     /locus_tag="PNS21_000506"
     CDS             complement(19864..20343)
                     /locus_tag="PNS21_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDI5158241.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20359..21111)
                     /locus_tag="PNS21_000507"
     CDS             complement(20359..21111)
                     /locus_tag="PNS21_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDI5158242.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21228..21298
                     /locus_tag="PNS21_000508"
     tRNA            21228..21298
                     /locus_tag="PNS21_000508"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21260..21262,aa:Gly,seq:ccc)
     gene            21664..22389
                     /locus_tag="PNS21_000509"
     CDS             21664..22389
                     /locus_tag="PNS21_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDI5158243.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22643..22909
                     /locus_tag="PNS21_000510"
     CDS             22643..22909
                     /locus_tag="PNS21_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDI5158244.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDSAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22906..23337
                     /gene="ruvX"
                     /locus_tag="PNS21_000511"
     CDS             22906..23337
                     /gene="ruvX"
                     /locus_tag="PNS21_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDI5158245.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23363..23665
                     /locus_tag="PNS21_000512"
     CDS             23363..23665
                     /locus_tag="PNS21_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDI5158246.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            23855..24691
                     /locus_tag="PNS21_000513"
     CDS             23855..24691
                     /locus_tag="PNS21_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDI5158247.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            24755..25186
                     /locus_tag="PNS21_000514"
     CDS             24755..25186
                     /locus_tag="PNS21_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5158248.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(25248..26570)
                     /locus_tag="PNS21_000515"
     CDS             complement(25248..26570)
                     /locus_tag="PNS21_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HDI5158249.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(26724..28364)
                     /locus_tag="PNS21_000516"
     CDS             complement(26724..28364)
                     /locus_tag="PNS21_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDI5158250.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            complement(28805..30124)
                     /locus_tag="PNS21_000517"
     CDS             complement(28805..30124)
                     /locus_tag="PNS21_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HDI5158251.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            30381..30851
                     /locus_tag="PNS21_000518"
     CDS             30381..30851
                     /locus_tag="PNS21_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HDI5158252.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            31073..32176
                     /locus_tag="PNS21_000519"
     CDS             31073..32176
                     /locus_tag="PNS21_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDI5158253.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            32397..33356
                     /locus_tag="PNS21_000520"
     CDS             32397..33356
                     /locus_tag="PNS21_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDI5158254.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            33526..34206
                     /locus_tag="PNS21_000521"
     CDS             33526..34206
                     /locus_tag="PNS21_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDI5158255.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            34296..35330
                     /locus_tag="PNS21_000522"
     CDS             34296..35330
                     /locus_tag="PNS21_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDI5158256.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            35344..35679
                     /locus_tag="PNS21_000523"
     CDS             35344..35679
                     /locus_tag="PNS21_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158257.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            35663..38143
                     /locus_tag="PNS21_000524"
     CDS             35663..38143
                     /locus_tag="PNS21_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158258.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            38199..39758
                     /locus_tag="PNS21_000525"
     CDS             38199..39758
                     /locus_tag="PNS21_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5158259.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            39755..41119
                     /locus_tag="PNS21_000526"
     CDS             39755..41119
                     /locus_tag="PNS21_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI5158260.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            41290..42360
                     /locus_tag="PNS21_000527"
     CDS             41290..42360
                     /locus_tag="PNS21_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5158261.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            42375..43043
                     /locus_tag="PNS21_000528"
     CDS             42375..43043
                     /locus_tag="PNS21_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5158262.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            43063..43641
                     /locus_tag="PNS21_000529"
     CDS             43063..43641
                     /locus_tag="PNS21_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI5158263.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            44042..44953
                     /locus_tag="PNS21_000530"
     CDS             44042..44953
                     /locus_tag="PNS21_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDI5158264.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(45331..46248)
                     /locus_tag="PNS21_000531"
     CDS             complement(45331..46248)
                     /locus_tag="PNS21_000531"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDI5158265.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            46450..46887
                     /gene="zapA"
                     /locus_tag="PNS21_000532"
     CDS             46450..46887
                     /gene="zapA"
                     /locus_tag="PNS21_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDI5158266.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            46945..47493
                     /locus_tag="PNS21_000533"
     CDS             46945..47493
                     /locus_tag="PNS21_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDI5158267.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            47608..49968
                     /locus_tag="PNS21_000534"
     CDS             47608..49968
                     /locus_tag="PNS21_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDI5158268.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            50055..50369
                     /gene="trxA"
                     /locus_tag="PNS21_000535"
     CDS             50055..50369
                     /gene="trxA"
                     /locus_tag="PNS21_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDI5158269.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            50551..52329
                     /gene="uvrC"
                     /locus_tag="PNS21_000536"
     CDS             50551..52329
                     /gene="uvrC"
                     /locus_tag="PNS21_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDI5158270.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            52474..53373
                     /locus_tag="PNS21_000537"
     CDS             52474..53373
                     /locus_tag="PNS21_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5158271.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            53366..54628
                     /locus_tag="PNS21_000538"
     CDS             53366..54628
                     /locus_tag="PNS21_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5158272.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            54789..56687
                     /locus_tag="PNS21_000539"
     CDS             54789..56687
                     /locus_tag="PNS21_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HDI5158273.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(57017..57736)
                     /locus_tag="PNS21_000540"
     CDS             complement(57017..57736)
                     /locus_tag="PNS21_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDI5158274.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            58073..58405
                     /locus_tag="PNS21_000541"
     CDS             58073..58405
                     /locus_tag="PNS21_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158275.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            58392..60395
                     /locus_tag="PNS21_000542"
     CDS             58392..60395
                     /locus_tag="PNS21_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDI5158276.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            60414..60884
                     /locus_tag="PNS21_000543"
     CDS             60414..60884
                     /locus_tag="PNS21_000543"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDI5158277.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            60905..61489
                     /locus_tag="PNS21_000544"
     CDS             60905..61489
                     /locus_tag="PNS21_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HDI5158278.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            61504..62490
                     /locus_tag="PNS21_000545"
     CDS             61504..62490
                     /locus_tag="PNS21_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HDI5158279.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            62480..62791
                     /locus_tag="PNS21_000546"
     CDS             62480..62791
                     /locus_tag="PNS21_000546"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDI5158280.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            62813..64594
                     /locus_tag="PNS21_000547"
     CDS             62813..64594
                     /locus_tag="PNS21_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDI5158281.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            64587..65963
                     /locus_tag="PNS21_000548"
     CDS             64587..65963
                     /locus_tag="PNS21_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDI5158282.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            65970..66605
                     /locus_tag="PNS21_000549"
     CDS             65970..66605
                     /locus_tag="PNS21_000549"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDI5158283.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            66635..67990
                     /locus_tag="PNS21_000550"
     CDS             66635..67990
                     /locus_tag="PNS21_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDI5158284.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
BASE COUNT        22285 a        11677 c        14066 g        20098 t
ORIGIN      
        1 taaataaata ggaacaaaaa aattaaatta tttaatacac acattaggga tcattcattg
       61 aaggattaga agacaacgag gatggcgaaa aatatgatta caatagaaaa gtcgttctaa
      121 accaatcagt tcctagtatc acacaacgca agccagtcga tttggtgaga ttagaggagt
      181 gaaagcaaac tttattatat aagaaaaaat attcgaaagg gatgctagtt taggtactag
      241 tgctgataga ttaatatgat gcctcgtaat attaggcgta gttactctag agacatcact
      301 atggactatg atttgtctca gagtcacaac atgttttttt atgaaaagaa acagctaaaa
      361 taattaaatt gcgcaatgtt taaactttct taaactataa cctaataaat gtgaagaaat
      421 aatgcacaat ttcttcacat ttatcttcta ttctatatac ataccaacca acaaccctat
      481 atactttttc atttttactc ctcaagtaaa aataactcct agaccgcggc tacccaccgc
      541 ggtcgttttt ttattcttca atcaaaatcg ctttgatacc caaacctgcc aagattcggg
      601 cagaagtata agtattcaat cctcttctga aagtaatgta aactttttta tcttttggga
      661 tttcattgat tcgatctcgt aactcattca ttggaatatt ttttgttgcc gagatactgc
      721 cagaaggtgc tttattaggt tcacgtatat ctaaaaagaa cgcttctttc aaatcgtttt
      781 cgggtatatc acttgctttg atcgtttcta cgatattcgt catttgattg attgcgacat
      841 aaccagctat attcaacggg tcacgggtag tagaatacgg tggagagtaa ggcaactcca
      901 aatcaggcaa atcaaagact gtcaagtttc cggtgattgc tacagaaagt tctcccatcc
      961 gtttatcgac acctttttct ccaaatgctt gtccacctaa aatacgaccg ctatctgcat
     1021 caaaaatcag ctttagattt aatcttgtag cttctggata gaaataggca tggtcaaagg
     1081 gagtgataaa aacagtttta taatttgtta tgcctgatgc ttttaattca tgttctgtca
     1141 ttcccgcata acttgctgta tgatcaaaga tttttgcgac acctgcacca atatatccgc
     1201 gatagcgcat aggcgtacca tttaaaatat ctgccagcat atgtccttga cgattggccg
     1261 cactggaaag catactttgg atcggctgac cagtcactac actagtcgtt tcgataatat
     1321 cgccaatcgc atagatgtcc ggcagattgg tttgtaactg gtcatcaaca atgatctgac
     1381 ctgtatctga taattgtatg ccggttgctt tcaccacttc attcttcgga gaaacaccaa
     1441 cagcaaaaat tagcatatca gcagaaagga cggagccatc tgataatact atttctcgac
     1501 ctttatcacg gatctcagta actcttgttt ctaaatgaac ggctaatcct tctttcagca
     1561 atttgtcata aactaaatta gcaatttctg gatcataagg gaaagcgact tgtggcaatt
     1621 gatcgattaa agtcacgtct aagccgcgat gcttgaaatt ctcagctaat tccagaccca
     1681 taacacctgc accaagaatg acgacatgtt gtggattttt ttcttcaagg aacgatttga
     1741 ttcgatccgc atccgtcaca ctacggagga caaagccgtt ttctgcttca aaaactccgg
     1801 gaatatccgg ataatctggt ctagcaccag aagagatgat caacttatcg taatctgttt
     1861 ttatttcttc atttgtggat aggtctttaa ctgttaatcg cttagtagaa ggatcgattg
     1921 ctgttacttc gtgtttagta aagacatcga tgttgttttt tgttttcaat atttctggtg
     1981 tacgttcgat cagcgagtca cggtcagtga tcacacctcc taaataataa ggcaaagcac
     2041 aacttgcata agaaatattt tctccgcgtt catagataat gatttcgtgc gcttcattca
     2101 atcgacggaa acgagtggca aatgaaggac cgccagctac accgccaatg acaacaactt
     2161 tcataaaaac atctcctttt ctacgttgat cgtactatgt tatggtaaaa aacagcaaat
     2221 aattgtgccg gaaaattatt gtttattttg aatagacaga aaatatagag aaataatttg
     2281 tggataaaac ctaaaaattt ttttctattt taaggataga taatactttg aaggggataa
     2341 ccctgtatat tttttaaaag ctgaagaaaa atgatggatc gaatgaaatc ctaatatatc
     2401 tgcaatttct gtataggtta attcattttc tagcacgaga cgttttgctt tttccatttt
     2461 tagttcttta aaatagctaa taacactttt tccattcact tttctaaaat gtttttttaa
     2521 gctggctgga ctgataaaaa aaatttgggc taattgttca agcaaaaaat tttctgaaag
     2581 atgttcttct aaatatcgta tgattggatc aactaattca tctgaataaa gaatatgctt
     2641 ttttttagga atgactttct catttgaagg tattgtcaat aaagcaagtt cctgttgata
     2701 atgccttggt aaggaagaca attgaatttt ttgcagacaa taagtaacta ataaggaaag
     2761 caaatgaatg ctcatctgtt ggcatagtat gaaaaaagaa ttgatttcct gatcatagga
     2821 aatagctggc ttactatgtt ttagataatc aattttgaac tgttcatttt tcagaaaagt
     2881 aatgatagat gttatttctt gatctataga aaaagattgt cccgtaatat gtgctaataa
     2941 ttgctcatca aaataaaact ggataatgat aaaagtttgt gtttcctttt gagtagaaaa
     3001 gatttggact tcttgctgtt tatcaatgaa taaaagggaa ggattatcta gttctataga
     3061 tttatgtgca aattcagcat ataaattagc gctttcattt attagtagtc gaaaataggg
     3121 agcagcagat aaatgaagat gggtatctat ggaataggtt agtgtttttt tgacaagatt
     3181 ggaggattgg ttgtttgtta acaaaagaac cactttcctt tctcttttta tataaaaagc
     3241 tttctattta taattaatga ctattataac aaaaatgaaa agccaaaacg acaagtaaaa
     3301 tattttttta tataaatatt tgtgttacta attccgttaa tatacataag tgaaagcgtt
     3361 ttatataaga acttgaaaag aaagaaggga tagacatgaa aaaaagatac aaattggtga
     3421 tagctttgct aaacattacc ctattattca gcttaagtgc atgtggaaaa actaacgaag
     3481 aagtaacagc aaacatcgca gatccaatga taaaaaaagc cactacatta gaagtatatg
     3541 tcgcaaacgg caaactgaca aactatgttg aaaatgctgc aaaaatctat aaccgcaaaa
     3601 ctggagcaaa tattgactta aaaattgtga atgtagcttc gggaacagca acagtacaaa
     3661 tgatcacccc aaaattggta acacacgaag atatgccgga tattatctcc atggcagaca
     3721 cttcagcagc aggtgtgctg gaaaaatttc aagatgcttt ttatagtgct gatgaatatg
     3781 gattttatga caaatatggc gatcagtttt atgatcaaaa attgaatatt ttgcgtcaac
     3841 aaacgagtaa tgaaacgatt attccttggg ctagtgattt taccccagca ctttgttatt
     3901 atcaaccaaa aaaatttgct gaaataggtg tagattttga agaaatcagt tcttgggaag
     3961 aatatatcca ggtggcaaaa gcattaaaag aaaaaactgg tagttttgga attgccttgc
     4021 cagaatctgg ggaccaagaa ttgtttatca acatgctagc ccagcagaat caatctttgc
     4081 ttgatgaaga aggaaatatc cgtctgaata ctaaagaagc gaaacatgct gcaaagttaa
     4141 tcaaagaaat gatcgactca gatattgtac atttctacgg tgcacaagat gctgagaaag
     4201 catttcaaga atctgcaatg tttgttgctg gcggctggta tgctacgaat atgtctttga
     4261 attttcctga tgcatctgac aaatggcgca tggctccact gattcctttt tcaaaagaaa
     4321 atccaggaaa aggtgcagta tcaggaggat caagttttta tgtccctaaa gatgccaaaa
     4381 atccacaagt ggcacaacaa tttcttacat ttatgctgac agatgaagaa tgcttagcca
     4441 acgcactgga gctgggcgtg gctacatcta actcattggc ctatcaaaca gaagcagctg
     4501 agaaaaagtt tgactactaa ggaggacaaa aatactatga actattgaat aaaatcaatg
     4561 aaaatacatc aaatactgtt ttaccagcca cttattcaga tgctacgtct tatttgacta
     4621 ccgctgcata taactattgg caaacaggtg attttgaaaa ttcatatcta aaagaagcag
     4681 aaaattttgc tcataagtac ggcgtaaaat taactggtga ttaaaaaata ttaggaggac
     4741 ttaacgatga tgcaatcttc gttaccaagc aagacaataa agaacagcaa gaaaaaagca
     4801 acagatacgt ctcataaaaa aagtgcttat ggattggtca caccttttgt tattttcttt
     4861 attgtgacga ttttgattcc catcattatc tcgtttgctt ttagttttat gaaaattggc
     4921 tactcatttg aatttgttgg atttgacaat tatctagcta tcttccgaga tccattgttt
     4981 attaaatctt acacgaatgt tttgattatc atggcaggat caattccaat aacgatgcta
     5041 ttagctattt actttgctgt tttattgaat tcagcacagt taaaaggaaa aggatttttt
     5101 agaactgtct attatattcc aaccattaca tcaacggtgg ctgtagcttc cgtattcatg
     5161 acttttttca atccgactgg gttattcaat ggatttttga atgcgctagg aattaacggt
     5221 gtacagtggt taacaaatcc tttttggata cgaatatcaa tgatcatcac gatgatttgg
     5281 cttagtgtag gatataacac tattttattt ttagctggtc ttcaagggat ttctaaagag
     5341 atatacgaaa gtgctgaaat agatggagca tcgaaatttc ggcaattctt tttattaacg
     5401 atccccatgc taaagccaat cattttaatg gccacagttt tagccactat caatggcttg
     5461 ggtaatttca atatcccaaa tatattcttt gggacttcca atggaccaga aaattcagct
     5521 ttggtcgtag gagtcaattt atataaaaca agttttgaaa tggttgattt tggaaaagcc
     5581 tctgctattg cctggacgat ggtgtttgta tctgttatat tatcggctat tcaatttaaa
     5641 ttcggaggtg ataaaaatga ctaaacaaaa gatacaaagc agaattttta cgtacgtcat
     5701 tattgtgttt ggtgccttaa tatttttgat gccatattta tatatgattg tagcttcaac
     5761 gcaaagtaat caagaaattt taagtggcgg aatcaatttc aagtttgggg atatgctatt
     5821 aaaaaactgg caagccatca tggcgagata taattatccc ttgattattt ggaattcgat
     5881 tgttattgct gtaatcagta cagtttcagc tacagccagt tctacaatgg cgggttatgc
     5941 attagcaaag tatcgtttta tggggaataa actgttgttc aatacagtta tgctatctag
     6001 gatggtgcct ttttttacca cattgattcc tttattttac attatgagtc gtttaggatt
     6061 atcgaatact tatgcaggtg tgatcatccc ttcgattgcc agtacaactt cagtgtttat
     6121 gatgcgacaa tatagcagtc aatttcctac agcactgatg gaggctgcca gaatcgatgg
     6181 tgccaatgag tgggtgattt ttacgaaaat cgctgtaccg attttatcac ctacgattat
     6241 tactagtggt ttacttatct ttatgggaag ttggaatcaa tatctatttc cattagtcat
     6301 gctttcagat aaagataaat atacggttcc cttaattatc aaaaatatga cggtttctag
     6361 tacaggtgag ccaattaatt atggcgcaat tatgctaatt ttagcaactt cagtgatccc
     6421 aatggtcttg atttatgtgt gggcgcagtc taaatttaag gagaatgaca ttggagctgc
     6481 aaataagtaa aaaaaataac gaatgttaac catctagttc gttctaggag caactccaca
     6541 ttcataaaaa ttaaaagaat ggcgggatat ccatgaatga acagacgaag gaatggtcaa
     6601 gaaaaatttt gaatgtagct tctgaaaaga tagatgcgga attagacaga atggggaata
     6661 aaattccata tatttcttac aacggaaaat atacagacgt aataaaggac agaggaatcg
     6721 actggtggac caatggtttt tggagtggta ttttatggca gctctttcac aagacaaaac
     6781 gagaaaagtt caaagaggca gcaatacaac aagaacaatt attagaatct gccctgtacc
     6841 atttttcaga cgttcatcat gatgtaggtt ttttattctt accatcggct attgcacatt
     6901 atcaacagtc tcaaaacaaa catagtcgac aactagggtt acatgcagct acggtacttg
     6961 ctggacgcta taatatttca gggaactatt tagtagcatg gaatgaccat aaacctggtt
     7021 ggatgattat tgatagtatg atgaatttac cattactgta ttgggcttcg agggaaactg
     7081 gtgatccgag gttctctaaa attgctcggc agcatgcaga tacaacagct aagtatctag
     7141 tgaggaaaga tggatcaacc gggcatatcg cagaatttga tgcagaatca gggaaatata
     7201 ttcggttact tggcgggcaa ggatacaatg agaattcttg ttggagcaga ggaaatgcat
     7261 gggctttata tggattcgcg ttaagttatc gttacacaaa aaatccgtac tatctagcta
     7321 tagcagaaaa gatagcagta aattttattc agaaaatcga aaaaactggt tatattcctc
     7381 tcattgattt tgctgtaaaa gattcgaata ttgataccag tgctggagta tgcgctgcat
     7441 gcggtcttct ggaattagca ggatatgttt cctctgtacg atcaaaaatg tatcatgaag
     7501 ctgctatcaa tatactccaa aagacagaaa agcactatgc aaattggaat atggatgaag
     7561 atgggatcat tggaggcgga gcagaagcat accatagaga actgacttat aatgtgccac
     7621 tgatctattc tgattatttt tttattgaag gcttacttcg attagaacag tcggaattag
     7681 ctatttggta gtaaaaagag gagaaaatac aatgaaacat ccagtaccac tattaaggac
     7741 cagacaggat tactttgact tatttaaaaa gataaatcaa ccactccagc cgttttatcg
     7801 agcaagtggc gcacaaattg attatataca tcaaggggta ggatatggaa ataaaattgc
     7861 aggaatggaa ggattctctc gggttttatg gggagcgggg cccgcaattg accaattaga
     7921 tgaacgatgg ttgaaattaa ttctatcagg aataaaagct ggaacagatc cttcccatcc
     7981 cgattattgg ggagaaatac atgaaagaga ccagcgtatg gtagaaatgc ccgcaatgtt
     8041 attagcgtta tatcatcatg aacaattatt atggaaaaaa ttagatacag gagaaaaaag
     8101 ccagattatt cattggttct cgcaaatttt tgaatatgaa tgtgcagatg gtaattggca
     8161 attttttaag ataattgtcg gaacaattat ccaaaaattt ggttatccag ttaaagaatc
     8221 agcactgaaa gaagcatttg ataatataga aaattgttat cttcaaaatg gctggtatcg
     8281 agacagttca agaggtcgcc aagattatta taatcctttt gcatttcatt attatggttt
     8341 gatttatagc gtactagaac cagaaaagcc cattagccag atatataaga accgagctat
     8401 tgatttttct agagactata ttcatttttt tgcggaagat ggtgcaaatg tgccttttgg
     8461 tcgaagcatg atttatcgtt ttgcagtttc aagcttttgg tcagctatgt tgtgggccga
     8521 tcttttgcct tttaaattag gtgaaatcaa ggggttagtc aatcggaata taagatggtg
     8581 gatgcaaaaa gaaatatttg atgaaaaagg tatcctgaat attggttata cctaccctca
     8641 attgacattg actgaaccct ataatagtac gttatctcca ttatggctga ataagatttt
     8701 tttattgttg gcgctacctg aagaccatga gtattggacg gctaaagaaa tgcctatgcc
     8761 tattcgtgaa ggaactaaac ttctttcaga agccaatttg ctcatacaac atgataatgg
     8821 acatacattt tttttgaatg cgggacaatt agggccaaat ttccatacat tgaccaatga
     8881 aaaatattta aaatttgctt attctagtca atttggtttt tcgataccgc gagcgaatca
     8941 attgaaagca gaaatggcaa tggattcaat gctagggatt cagcgagcgg acacatatat
     9001 cacgaccagt tttaagaatg agagcatcaa aacctatgga cagttcatcg ttcgaaataa
     9061 tgtacgagac attgtatgta agaaagaggt acttgcctct acatgggaac ctactttaca
     9121 ggcaaaaatc agaacttggc ttattccttt tgaaggctgg cagattcgcg tacacaaagt
     9181 agaattagat acagaatatg ttctatatga gacagggttt gctattgaat gttctcctga
     9241 gaaagaagga ataatcatag aagaagacaa ggaaaactat agggtgagtg aagctggctt
     9301 ttcaggtatt atctgtttaa gtgacgatac tattaaacga aaatccacag ctattatggg
     9361 tttgcccaat acaaacttga tgacatggga aaacactttt attcctggat tagagggtac
     9421 atttggaaaa ggaacccatt ggctcggaac aggagtggtt gctcatcaag atcgggatta
     9481 tagtttagaa gtatggaaca atcgtcccaa aattgatttt gatgggacaa ccgggctaac
     9541 tatccaaaat aaaaacaata aaaaaagact aaaattatgc taaaggctgg tggggaaatg
     9601 cttgataaag tgatgtttga agagataata catccagaaa aatggataaa tgaatgttat
     9661 aaagaattaa tggaaaaaga atggaataat gagatcgagc gttgtcgcag acacacggag
     9721 aggttaatca aacgtatcag taacagtcag tatcaattcc caagcgagtg tcctataaaa
     9781 ggacgttatc cattaagaga caatattggt tggacagaag gattttggac aggaattaat
     9841 tggttgttat ttgagaagac gggtgatcct ttatttcaac aaacagctga aaaagcagat
     9901 atagatttta tccacaggat tgactgtcaa attatcacta atcatcacga tcttggtttt
     9961 ttgtattctt tatcaaccgt agcagattat aaaataacgg gaaatcgaaa aatgaaggaa
    10021 gcttcaattg atgctgcaga aacattactt cagcgttttt gggagaaggc aggagttata
    10081 caagcttggg gagatcctag tgatccttca caacaaggta gaatgatcat ggattgttgc
    10141 atgaatcttc cattattgta ttgggcgagt gaagaaactg gaaatcaaaa atataaaaaa
    10201 gctgctcagg ctcatattca aaatgcagca gattatttag taagagaaga tggttcaact
    10261 tatcatacat tttatatgga tgtcaaagat ggaaaaccaa gatatggaag tacgcatcaa
    10321 ggttatagtg ataattcctg ttgggctaga ggccaagcat gggggattta cggttttgca
    10381 ttaagttatc gttatttgaa agaacaaaaa tattttgatt taagcaaaat attagccaat
    10441 tattttatga atcggttgcc agaagatacc atttgttatt gggatttgat ctttgatgat
    10501 tctagcagag aagaaaaaga tacatctagt atggtgatca tggtttgtgg attattagaa
    10561 ttaatagaat caggtctatt agacaaaaca gaaaagaact tataccagtc tgctgtttgg
    10621 aaaattatgc gacaattgaa agaaagatat gctacaacgg aagaagaaga tggcttttta
    10681 acacagtctg tttataataa gcccaaaaat aatggggtaa atacaccttg tatttggggt
    10741 gattactatt atttagaagc tttagtgaga atgactagta attggtcttc ttattggtga
    10801 aaaaggtaaa tatggaattt ttgtttgata aaataaaaaa agaaaaacca aaacagcatg
    10861 aatggtttct gttttggttt tttcgttaat tttcatattc ttctaattcc aagcttagtt
    10921 cttcccattc cgtcaatttt gtttcttgat cttgtctagc agcttctaaa tcttggttca
    10981 gctcattgag ttttacgtga tcttccaggt tttctggcgc gaccatttct tgttccaact
    11041 gatcgatcca ttcatcgatc cgcgccattt cttcttcaac ctgagtaatt tttcgttgaa
    11101 ggctacggat aagcttttgc tgttctttgc tttggtaata atcagatttg ttcgtgggag
    11161 caggagcaga ttctggctca tcttgtgcca acaattcggc gatttcttct tcttcctgct
    11221 ttttctctaa ataataatca tagtctccta gatagacttt gctgccattt tcagagagtt
    11281 cgacgacttt tgtcgcgatc cgattgatga aatatcggtc atgggaaaca aaaagcagtg
    11341 tgccgtcata atcgatcaat gcattttcta atacttcttt gttgtcaata tctaaatggt
    11401 ttgttggttc atccaaaatc aaaaaatttt ctttgttcat cgctaacttg gctaaagcaa
    11461 cccgtgcttt ttcaccgcca cttagcagag ggattggttt ttctacatct tcgccagaaa
    11521 ataagaaagc acctaaagca cttcggatct ctttttctgg agtggtagga tgctcatccc
    11581 acaattctgc taggatcgtc ttgtttgtat gcagatcagc ttgaccttga tcgtaatagc
    11641 cgatggtgac atttgtccct agcgtttctt ttccacggat aaaagggatc tggtgaatga
    11701 ttgatttcag caacgtcgat ttcccgatac cattcggacc tactagagca atcgcttctt
    11761 gacgacggat atctagattg attggttcag ataaggtgac attatcatat ccgatagcgg
    11821 catcttcgat ttgcaagact acatttccag aaggcttttc tatactgaat agaaagtttg
    11881 cttgtttttc atccccttga ggacgatcca gccggtccat tttttctaat gtctttctgc
    11941 ggctttgcgc tcgttttgtg gtagaggcgc gaacaaggtt ccgagcaacg aagtcttcta
    12001 atttatggat ttctgtttgc tgcttttcat aagccttcca ttcacttgct aactgttctg
    12061 ctttcaaatc tagatatttc gaatagttac ctttgtaatg gcgcattttt cttctgctta
    12121 attcatagac ttcattgact actttatcta aaaaataacg gtcatgggaa acaatcaata
    12181 atgcaccagt atagctttgc agataattct caagccagga aagtgtctcg atatctaaat
    12241 ggttcgttgg ttcgtctaga atcaaaatgt cagggtgttg caaaagcatg cgagctaaag
    12301 ctaaacgtgt gcgctgtccg cctgataaag tatcgatttt ctgatcataa aatgaagaat
    12361 cgaatttaaa cccatgcaat acggaacgaa tttcattttc ataaccatag ccgtttcgat
    12421 ctgaaaaatc gtgttgcagc cggtcgtatt cttttaaata agaactataa gcaggtgtgt
    12481 ctggcgtacc ttcgctaatg aaaatctcta gctcgcgcat ccgctcttcc atttgtctaa
    12541 cttgttcaaa agctttcagc atctcgttcc aaactgtttc attggaatca agtcctgtgt
    12601 cctgagcgag gtatccgaga gtcgctgttt tatttttagc gatctttcct tcatcaggtg
    12661 cttcaattcc agcaatgatc tttaataaag tcgattttcc agcgccattt ctgccaacta
    12721 aagcgacacg agcgcctgta ctgatttcta aatgtatgtt ttcaaataag acttctgcac
    12781 caaaatggcg agcaatctga ttggcttgta ataatatcat ggaatcctct tttctacttt
    12841 gtgaattctc ctcaaataaa aaactatttt tcaattagta gtctaccata aattccatta
    12901 tactgcgaga tttgcagaaa tgataaataa atgaataaag aataaaattt cacaatatct
    12961 gagcaatgga attgattttt tcctataaat actgctatga tggagaaatc aaagatgtaa
    13021 ttagacaata cgtggaggtc atttcgtgaa agatcatatt attccaaagg cgacagctaa
    13081 aagactgcct ttgtattatc gctatttaag gattttaaat aatgctggaa agaaaaaagt
    13141 gtcttcaact gaattaagtg aagccgttca agtagatagt gcaacgatcc gtcgagattt
    13201 ttcttatttt ggcgaattag gaaaacgtgg gtatggttat gatgtagaag atcttatgca
    13261 cttttttgca aagacattaa acgacgatca attgacaaat gttgcgctga tcggtgtagg
    13321 gaacttggga agtgctttac taaaatacaa attccatcaa agtaatagca tacgtatcag
    13381 ttgtgctttt gatgtgaatg aggaaatcgt tgggcgcatc gtagacggta tccccgttta
    13441 tccaatggaa gatatgtcag aacaaatcaa gatccaacaa atcgaagtag caattttaac
    13501 gatacccgct gaaagagcac aagaagtagt caatcagtta acggaagcag gaatcaaagg
    13561 aatcttgaac tttaccgctg ctcgattgaa tgctccgtca gatgtattga tccaaaatgt
    13621 tgatttgacg aatgaattgc aaaccttgat ctatttcttg cattctgacg caaatgcgca
    13681 ttccgaaaat acagcagaat aaaaataaaa agcgattgaa caaatgaagg caaaactttc
    13741 acttgttcaa tcgctttttt ctgtttggac aacgattatt ttgttcggaa gatttcttca
    13801 taagaaaggg aatcatcttc tgctgtcagt tcatctagta gataacggat ttttttaaag
    13861 tcgatgaacc gtgcttgccg aaagacttct tttccttgat gaaaaatcag gacaactggc
    13921 gcagtcagca cttcaaattt actagctact tctggtgttt caaatgcatc caaatgaagt
    13981 gccggaatat cataatgtgt gagaagttcc tctagtcgtg gacgaacagc gtgacaaaca
    14041 ctgcaattag gcatcgaaag ataaatgacg gcaacggatg actgttgcag tatgtcttga
    14101 acttcagtca agttagtggt ttgtttcatt tttattctcc cttttatgat agaagtgata
    14161 tgtatgagac cttaatttaa tgggaataac agaaaaagtc aattgaagtg ctttggtaaa
    14221 aaataagagt tgtgtaaaaa tttacagagc aacttgtttt ttttgaataa aagcgtatat
    14281 ttgcaaagcg aatgaactag aaataaaaag gaggaaactc atgttttctt ttattccaaa
    14341 ttcggttttg cagatgagta cgattttctt atcgatcgtt attgaagcat tacctttcgt
    14401 attattaggc tgtcttatct caggcgcctt acaagtattt ttgaccccag aacgtgtgaa
    14461 gagatggctt cctgaaaatc gtatcttctc tatttttact ggttcgatct taggattttt
    14521 ctttccttca tgtgaatgcg gcatcgtacc gatcgttcat caatttgtta aaaaaggcgt
    14581 accagttcac acagcgtttg cttttatgct gactgctccg atcatcaatc caatcgtgat
    14641 tttttccact tttatcgcat ttggcaattc ttggcagatg gcaagctggc gggtgatagg
    14701 cagctttttt gttgcccttg tgattggtgt ctggcttgcc tattttcaaa aagacgctgt
    14761 attgaaagaa aaaatcacgt ctaaagtagc agaaaatcac catcatcatg tccatgaaga
    14821 agaacaaagt gagaagaatg aaccaaaaaa tcagctgatc catcaaactg ggcatgtgtt
    14881 gacacattca atcgacgaat tttttgatac aggtcgttat ttgatcattg gcggattaat
    14941 cgcagctgcg atgcagactt atttaccaac tagaattatg ctaacgctcg gctcaacaaa
    15001 gattttagcc attatcatca tgctgttatt ggcctttacg atgtctcttt gctcagaagc
    15061 agatgcgttt ataggatctt ctttgttaag tctgttcggc acagcacctg tagctgcctt
    15121 tttggtcttt ggcccaatgg tcgatatcaa aaatctactg atgatgaaac gatattttac
    15181 aggacgcttt attttatcgc tgatcggact gattgccctt agcgttattg gattcacatt
    15241 atttatttag gagggaaaat atgctgcggt ttttgatttt atctgggtat ttcgaattga
    15301 tgatgtactt gcaagtttct ggcaaactcg atcaatatat caatgtccat tatcgctact
    15361 tagctattct ttcaatgatt ttatctgctc ttttggcttt agttcagctg tatttgtggg
    15421 tcaaaaacgg aaaagcagaa gaaaaggcac acgagcacga tcatgaccat gggttgtcaa
    15481 aaccttatca acgtggaatc gcttatgtac tgctagcttt gccagtaatc gtggggacgt
    15541 tgtttccaac agttagtctc gacactacga tcgtagaagc caaaggattt aatttcccag
    15601 taagtaaaga atcagtaggg gatccggaga tgcagacaca atacttaaag ccggatacca
    15661 gtatttattt caacaaatca gactacttag accaaatgaa tgagttgaaa gaaaagtatg
    15721 gggataagca aactatcaag atcacagatg aaaactattt agaagttatg gagctaattt
    15781 ataattaccc tagcgaattc attggcaaaa aaatcagcta tgaaggattt gtttaccaaa
    15841 caccgaacga tagcaataca gatatcttct tgttccgatt cggcattatc cattgtgtgg
    15901 cagattcagg agtctttggt ttgttgacgc atatgccgga aggagtaacg gttaaaaatg
    15961 atgaatggta taaaatagaa ggaaccatcc aatcagacta ttatcaacca ttcaagcgtg
    16021 aaatcccatc tgtagttgtt acaaacgctg agaaagtctc tgctccgaaa aatcagtatg
    16081 tgtatcgttc tttttaaaag aacaaaataa ataaacatag ctcagacagc tgacacgcaa
    16141 ggacgtcggt tgtctggctt tttttgcatg atgtattcat ttgtttgata tttattttag
    16201 aagcagcatc ctgaaagtat ttgatatcgg tttcaaaggt gttggaaaag attgtgaaga
    16261 gaattacaat ttattcctaa catgtatgtt gaaaagttgt atgaaatgat tttgatagta
    16321 aaatatcgag tggaggtact ttttatggta acgcgtatat ttattttgga tgatagtttg
    16381 gtttttgaaa aaatgctagt agaaattttg gaagaatcca gaattttatt aagtccttgg
    16441 aaatttgaga tcgttcaggg attgaacgtt atacaatttg ttgaattcat taaaaataat
    16501 gaaatctatt cctcagatat ttttttccta gacatcgatt tgaacgccta ttatagcggt
    16561 atcgaactag gcgaaatgct tagagaaaag aatgaaaagt gtaaaattgt ttatctaacg
    16621 tctcatccga ataaagcaat aactgttatt aatcgaaaga ttacgcctag tggttatttg
    16681 ataaaagaat tcacttatga aaaaaataaa aaaagcatcc aaagttactt attgaaagaa
    16741 aaagaaaact atcctgttca acatgctacg aaaaaaaggt ggacaactgt ccgttatggt
    16801 aatagcgatc actatatcca ttacgatgaa atcctttatc taatgagcat gccaggttat
    16861 aaaaataaaa taggcctagt attgaaagat gaagagattc tcgtaaacgg cacaattaaa
    16921 aaatataaaa aatcactcga atatgagtat ttatgtaaag atttaaaagc ttatatcatt
    16981 aatactgaaa acattacatc tattagcaga gtaaacgaaa tagtaacatt tgcaaatgga
    17041 gaacaactag agatgggaaa gagaagtatt gataaactaa agaattttat gcaagaaaag
    17101 atagaaaatg aatcatttta aagaaaacaa agactcatat cttacctgtt agaagagtgt
    17161 tttcctgtag aagagaacaa cccaagtagc agcctagtcg tttctatcct tttttgttat
    17221 gcactctttt tgacatttac tccaccaata acgaagggta caataaaaag taataacaaa
    17281 caaaaggagt acagaaatga ttgaattcat caatgtaagt aaaagttacg ggaaaaaaca
    17341 agcattaaaa gacttgtctc tctcgataag acaaggggaa atttttggat ttttaggaca
    17401 taatggtgcc ggaaaatcaa ctacgattaa aagtttagtt agtattattg aaccatcatc
    17461 gggaacgatt ttagtagatg gaatgaaatt gacggaaaat cgattgagta tcaaacaaaa
    17521 aatcggttat gtgcctgatt ccccagatat ttttttacaa ttgacggctg gagaatattg
    17581 ggacttgatt tcagctgcct atgaattaga cacacaaaaa aaagaaaagc gtttagcaga
    17641 attgacagcg ctttttgata tgtatagtca tcaaaatgaa acaatcgcaa gtttttcaca
    17701 tggaatgcgt caaaaaacga ttttgatcgg cacgttgctg ccagatccag atatttgggt
    17761 attggatgaa cctttgcaag gacttgatcc tcaagcagca tttgatttga aagaaatgat
    17821 gaaggctcat gccgcaaaag gaaaaacagt cattttttct acacatgtat tggataccgc
    17881 acaacagtta tgcgataaac tggctatttt gaagaaaggt gaattgattt atcagggggc
    17941 agttagtgaa ttattgaatg gctcacctga tgaaacgcta gaaaagattt atttgaaaat
    18001 ggctggaagg caagcgacag gagatggacc gtatgtataa gcgacaatta ttagaattaa
    18061 ttcgagttaa tcttttatat gttaatccac agctcacgaa taaagcaaga gagaaaggaa
    18121 aaaagggaaa gcgattaact atctatcttt tgtctcaaaa tcttttttca gggctgattt
    18181 ttttactgat ttacggattt gctatgtttg ctatgaactt tgttaaattg ccaggtttct
    18241 ttacttatta cgtggcactt tttggtatgt taggtttaac tcaaagtata tcagtcattt
    18301 ataatatctt ttttgaagga aatgacctgc aagtattttt gcctctgcct tttggacaag
    18361 aacaggtttt ttcagcaaag attttggtag ttgctttgac catcattcct tttgtttttc
    18421 cgcttctagt catgtttatt ttaaccggat ggcgagctga tctagggatt ttgcttacta
    18481 tcattatctc aatccttttg ttttctctat ttttgattat tcttttttgt ctggctagtt
    18541 tgattgtttt tggtttggct cgaacagcag tgtttaaaaa acatagaaaa acagtttcta
    18601 gtatcttgct tggcagctcg atgctgatcg catttgctgg aatcatgtgg atgaatcatc
    18661 aaacggggac tattgaaaat gagttaacag atcggcatcc gatttcgata ttattgccat
    18721 tctatgatgc tgccagtcag ccactttctc aacaaggttt attgggttgg ggaatactag
    18781 ctatcacggc tctgggattt ttccttgctg taaaacagct gatagtacca aagcttacag
    18841 agcaactgac tgattcaaac agtgagttca aaataaaaag aacccataaa aagaaccaaa
    18901 atgtgcatca gcttttattt agttacaatg ctcaattagt aaaagaacct aatttgatta
    18961 tgcaagttct ttcttcttcc ttgttaactc caatcatttt tattcttgct tttgccctag
    19021 gaggcgaaat aaaattaact gatttagata ataagttaat cggtgttgtt ttcctggtag
    19081 ggatcgcatt ggctttctta acagttaatc agacttcttt tatcagcaat cttatttctt
    19141 tggatcaaga aaattttctg tttatccgtt ctttgccaat ctctatgaac caatatttaa
    19201 aagaaaagtt cagattcgga tggatattgc agagtttttt aactggtggg atcgcattat
    19261 taagcggact gtcttttcag ctgcctttgt tttttatccc tagcttgcta tttggttctt
    19321 tattcggctg ctacttattg tcactacgtt atttcgctag agattatcgg ttgctgctgc
    19381 tgaactggac caatattaac caattattta acagaggttc aggaagtttg ggtttagtcg
    19441 caactatgat gggttcattg atcatcagta ttattcttat cgtcttatac ggaatgttgg
    19501 ctttgtttta tccattttgg ttagtgaata ttcctgtgat cggtttagtt ctaatacttt
    19561 ccgctctatg gatcgtttat tatcaacgaa cgttttggaa aaaatttgaa taaagaagag
    19621 gggccgtgac aaaaatcatg tcgcagcccc ttttttacag atagagggtg ttcaaaagtc
    19681 agtttctcag tattaagtca attttatgta aaaatgtgag aagctatttt tacaaaactt
    19741 tttttaatac tcataattac ttcttattgc tcctgcggtt acttgtcgca atgtttgtga
    19801 gatattttgt catcgctcaa ggctgaacgt ctctttcaca accttttctt tgttacactt
    19861 cgtttattta aaaagccgtt tttttcgttt catcatgatt gcgtcacgct ttactttgaa
    19921 attacggatg ttatggttaa agacttctga taatacaaga cctaaagcga ttgctccagc
    19981 aatcataatt gcttggaccg cataatgagc cgcttctcga taggattcag taacgagatt
    20041 tcgaaccgct tgataagcta aaccaccagg gactagagga accatcccgg gaatattgaa
    20101 aatcgttacg ggcattttca aacgtttaga gaaaagatcg ctgatgaatg ctactcctaa
    20161 agaaccaagc aatgaaccaa aagcagcaga tccgcctatt tgaacacaga tccagtagat
    20221 catccagcca aaagcaccag aaagaccaca acaaatcaat gaacgcctag gtacatttgt
    20281 aataacggca aatgccgcag tcgctaaaaa actaaaagaa aactgaataa atatcgtccc
    20341 catacttgtg ttcctttcct aataaaaaag ctggaaaata aaagcaatcg caaaaccgat
    20401 catcgtagca gtcatcatcg cttctgttcc gcgagaaact cctgagacat aatgtccagc
    20461 tagcaaatca cggatagcgt tcgtaatttg aactccagga actaaaggca tcacacatcc
    20521 gataatgatt agatcctgat tgcttcctaa gccaaaacgt acgctgtaga tagcggctat
    20581 gccaatcaat aaagaagata aaaattccga taaaaatttg attctaaaaa atttcaaact
    20641 atatagataa gctgcgtatc ccaatcctcc aatgactaaa gtcacaggta aatcggtcat
    20701 ctttccgcca aataacacca taattgttcc actgatcact gccgcgctaa cgatttgcaa
    20761 ccagacagga aagaaagaac ggtctcggtc gattgcttgc agttcgcagt aaagttcttc
    20821 taatgttagc tctgaagcaa catatttacg tgaaagatca tttactttta ctactttttc
    20881 taaattaatc gaacggttgg taatttgttc cattcggatg gtcgaggtac gatcaagacc
    20941 aataaagaga ccggtttgag tgacatagct gaccaaacgg aaatttccag aagctaaagc
    21001 aattcgactc atcgtatctt ctacccgata catctccgca ttgctttcca tcatgatttt
    21061 cccagcgagt aaacaagtgt ccagcaacag atctaattct tttcccgtca tagcatcttc
    21121 tccaataatt gattgattag ttttatttta ctacaaaaca tatcggaagc gtaaagaata
    21181 aaaaaacttg aggaaggagt gaaagaggcg tatactgttt ttgtatcgcg ggtgtagttt
    21241 agtggtaaaa tcccagcttc ccaagctgat gtcgcgggtt cgattcccgt cacccgctta
    21301 aaagaggctg tgaaaaagct gccctgcatc aagtaataag aatagtcaag caaaaaagct
    21361 tcttatgttt ttgacttggt tggacttatt accgcagatg caagcttttg aacactgttt
    21421 accaagaggt cgtacagaag ccgtttagtt ttgagtatta agaaaaattt tttgaatgat
    21481 aataaatacc ttgtaaatag tgttttgtga ttgagtagag acgatgaatc cgtctctttt
    21541 tttgtattct taagccgtct aaaaactggt tttatttttt tactgtttct atttctttcc
    21601 agtatttatt tataggggaa catatgttaa gatttaggga actgttagta aaggagtaaa
    21661 gaaatgagca aaaaaacaac caaggcgatc gtggggatcg tcgctgctgt gattgcaggg
    21721 gcactcggta tttccaccac acaaaaagat tcagatatct tccaacaaat tacaaccatt
    21781 tttgattcca caccgcaagc ttctcaagta gcggatgtgc cagaatatga tggaaaacat
    21841 caagaaatag aaatcaatgg gaatcagccg gagtttacaa aagaggatct tagtctttcc
    21901 aaagggacat gggaaagcta ttcaaatata gacaaattga acagagtagg acaagccaat
    21961 gcgatgttgg gaaaagactt gtttcctact gaaaagagag aacctttgta tattgatccg
    22021 acaggttgga aacaaaagaa attaagtgac ggacaatggt tatacaaccg cagccatttg
    22081 attggtttcc agttgactgg tcaaaataat aacattaaaa acttaatgac agggacacgt
    22141 tcgttgaatg ctccgtacat gcttgcccac gaaaatgata ttgcggcata tatcaaggaa
    22201 acaaatcatc atgtacgcta tcgtgtcact cctcatttcg agggaaatga actggtagca
    22261 agaggtgtgc agttggaagc agaaagcatt gaagacaaaa aaattgaatt caatgtcttt
    22321 atctacaatg ttcaagatgg ctacaccatc aattatgaga cagggcaagc aacgaaaacg
    22381 aaaaattagt agcagcaatt cgaatttttt atgaacttta tgaaaaaatt gtgtaaaaac
    22441 atactttcaa agaaatatag cgtatacttt atggaaagag ggcttttgaa aaagccacag
    22501 aaaaaaggaa aaatcagaca aatcgtgttt cttaatttat tcagaagttt ggaatatttg
    22561 tttgtagttt tccctttctt cgagtaaaat aagggtgtag aaggtaagct ggaattacct
    22621 tgcgggaaag aggtgtagaa tcatgggttt tacagatgaa accgtacgtt ttggttttga
    22681 cgatagtcgc aaaaaagaag tgagcgaaac attagcgatt gtttatcggg ctttggaaga
    22741 aaaaggctac aatccaatca atcagattgt cggctacttg ctttctggag actcggccta
    22801 cattcctcgt tatcgtgatg cacgaaattt gattcgccgt cacgaacgcg atgaaatcat
    22861 ggaagtaatt gtcaaagact acctctcaaa tcatggagtg aatttatgag agtaatgggc
    22921 ttggatgtcg gttctaagac agtaggcgtt gctgtgagcg atccctttgg ctggacagct
    22981 caaggagtag aaatcattcg aatcaatgag gcagaagaag agtttggttt tgatcggttg
    23041 aaggaattag tggcgcagta cgaagtagac cgttttgtag ttggattacc taagaatatg
    23101 aacaattcca tcggaccaag ggcagaagct tccatgtcat atggagagaa gatcaaagaa
    23161 ctattctcct tgccggttga ttatcaagat gaacgactaa ccactgttca ggcagaacgt
    23221 atgctagtag agcaggcaaa tacttcaaga gcgaaacgaa aaaaagtcat cgataaatta
    23281 gcagctgtga tgattttaca aaattattta gacggctcag gtaagcaatt attttaataa
    23341 taaaaagaaa agaggtaatc ttatgacaga acataaccat tcccacgaac acgaaggaca
    23401 cgaacacatc acattggtgg atgaccaagg aaatgaaaca ttatacgaaa tcttgctaac
    23461 agttgacgga caagaagaat ttggacgtaa ctatgttctt ttatatccag ctggtgtttc
    23521 agaagatgaa gatgtagaat tacaagctta tgcatacatc gaaaacgaag atggaacaga
    23581 aggcgagtta gaacaaatcg aaacagacgc agaatgggat atgatcgaag aagtattcaa
    23641 tacattcatg gcggaagaag aatagaacaa aagaatcgat aaggtgtaag aaaagctgga
    23701 ctctctgaaa agtagagccc ggttttttcg tttagcacta aatttcctta aaaagtagaa
    23761 aagctttcga agcctcgttt gtttctttta ctgtataata aaagaaaaga gtaacaatcg
    23821 ctaaaagata taaacaagag aaaggaaata caagatgatt ggtctgattt attttgtggt
    23881 gatcgttctg gcgaacacag ttggcgctat ttccgggatg ggtggaggcg tgttgatcaa
    23941 accgattctt gatcttatcg gcgcccattc tgttgcaggg atttcttttt actcaacagt
    24001 tgcggtattc acgatgtcca ttgtttcgac tgtgcgacaa gtatcaagcg ggaaatcact
    24061 gaattggcag attgtcggtt gggtatctag tggagctgtt gttggaggag tagtcggcaa
    24121 cattgtattc gaagtttttt tacagttatt tgaaaacgag aagcatgtgc agctgatcca
    24181 aatttttttg acggtattga ccttggtttt tgcgttcttc tacaccaagc atcatcagcc
    24241 aaaatttcat ttgacgtcat ggacttggta ccttatatgt gggggggttc tgggcttttt
    24301 agctagtttt ttaggtattg gtggtggacc agtcaatgtt tctttgttga tgctgatgtt
    24361 tgcattgcca ataaaagaag caacactata ttctctatct acgattttct tttctcaact
    24421 ggcaaaattg gtaacgattg ctttaacaag tagctttatg cgctttgatt tatctatgtt
    24481 gttttatgtt atccccgctg ctgttattgg aggtttgtgg ggagcaagat ttagcagggt
    24541 tttgtctcca aaaaaagtga cctttatttt tcaagcgatt gtcatcgtgg ttcttttgat
    24601 caatctttac aatgctttcg tcatcttgca aacattagaa ggatctggat tcaagcaagt
    24661 caaaatcact gaaaagtctt tttttaagtg aaaaaaggag tatactgtga taaaaaaatt
    24721 catttaaaaa aataaaaatt ggctggtgat ttggatgttg cgggaaatgc gtttaggaga
    24781 tgcagaagca ctacgagaaa tcaatgcgga acaattagga tatgatgtac cattaaccgt
    24841 tacggtgaga caaatgaaga aactgttgaa ggagccagaa aaaaattttt tcttggtcta
    24901 tgaagatcct acttctaaaa aagcagctgg ctatgtacaa gcggagtcct atgaatcgat
    24961 tttcagcgat ccgatgttca atattatggc acttgctgtg tctaaacatg ctgaaaaaag
    25021 aggaatcgga aaggccttga tgactggcgt agaagaagcg aaaagacgag gaatatcagc
    25081 aataagactt aattctgcag aatacagagt agaagcacac cgattttatg aacgcatcgg
    25141 ttatcattcc gataaaatgc aaaaaagatt tttgaaaatc ctgtaaattt ttatcaaaaa
    25201 atacctggtc atcaagccga tacttgatga tcaggtattt tttttgacta agaaagtaat
    25261 tgcatcaaga aacctgagat gatcaaaacg atggcaccac caagccgatt gcccatttga
    25321 gcaaaagcaa taagttccat ccgattagct gcagataaga cagctacatt tcctgtacca
    25381 cccatcgagt tgttgcataa gccagcagta atagttgatt cgattggata taaaccaaac
    25441 aaacgaccga caaatcctga aactactgaa atcgcgataa cacttgtcaa acataagaca
    25501 acaaattgcc atgtcaaagc agaagccaat acatttagat tcaataacgc aataccgata
    25561 ccggcaagaa cagctgctgt caagtttttg acaatcaagt tgttgaacat gatagctgca
    25621 tcttcgtaat attctggtaa taggtttgcg attttacaga taacaacgat aatgatcatg
    25681 aatgcgtaag catggacttt aggaacaaaa tagttacaga tttgtcctaa gatgaagaaa
    25741 gtcaatgata ccatcaaacc gacacctaat tgtggaatgg aaggttttac atttctaggt
    25801 tttccatcgc caaggtcgcc tttttccatt aatttaccat taccgttgaa ttttggcatt
    25861 gattgtcccg cacgagcaac taacgctgca ccaatgatag ctaggacatt tgtcattgct
    25921 gtcgcaggaa tcaggcggga aatgatagaa gaagcgtccg tacctaaaac atttgcataa
    25981 atcgttgata aaggtacagc tccggctcca acaccaccag ccattgcggg caatgagata
    26041 tataaaacgg aatttgcaaa accatttccg ataagcatac ctacgagtcc tacggcaaaa
    26101 aaggagacga ccattgaaat aagtgcaacg ggaataaaac gaacagaagc tttcatcaat
    26161 agattacggt tcataccaag gatgctacca gtaatcaagg cagcaatata aaagtctaaa
    26221 aagcccatat tgctgacaaa gttttctgtg gattttacta catactcagg taagatccca
    26281 aatgtagcaa tcgcggcaga ggcaaagatc gcaaaaacgg agccaccacc taaataactt
    26341 ttgactatag ggatttttgt tccgataaaa tgaaataaat taccgaaaat gaccaaaaca
    26401 gcgattgggc caatcatatt cgtcggtaat tttcctaaag ccattgcaat gatcaggaca
    26461 aggaccatga tcaaataaag gggcaaactt acgccggtaa tttttgttga ccaaaaccct
    26521 tcttttttct tttcctcaac tgcagttgcg gttgttgttt tttgtgccat ttttacagat
    26581 cctttcttat tctgtatcgt atgaaaaaag ccaaagagaa gcggcaaaag agcagtagtt
    26641 ggctggctgc tgttttgccg cagtcgcttt tttcttttta gacgtcatac aaagtttctg
    26701 tatgttcttt tactataaca atcttaatct tctaaagatt tatattcagg aacccattta
    26761 gcagattcaa cagcttcttt gatatccgtg atttcttcac gattcaattt ttgtgcaaca
    26821 actgattttg cgacagtttc agcaatcgtt tgagagaatt cagtgatttt tgctactgga
    26881 ggcaagatag ctgctcctgg ttttgttaca tcaacgattc cgcctaatgc tcgactagct
    26941 tgagaaatga tttcagcatt tactcgtgta gctgttgaag caatcaaacc aagccctaaa
    27001 cctgggtaca tcaacgcatt atttgcttga ccgatttggt aagttacacc gttatattcc
    27061 acatcagcag cagggatacc tgttccaact aaagcttttc cgtccgtcca ttcaatcaaa
    27121 tcttttgctt ttgcttctgc caattttgtt ggattagaca aaggcatgat gattggacgt
    27181 ggggtatgag ccgccatttc tttcacgatt tcttcagtga atgctccagg ttgggtagaa
    27241 gtaccgatca tgatcgttgg atggattgct tttacaactg cttctaagtt tgtcaattca
    27301 tcagcattag tgaattctga gcgtttacga gcgaatggga tttgaccagg agtcaatcct
    27361 tttgtatctt caaacaataa accctgttta tctacttgat agaaatgttg gcgtgcttct
    27421 tcttcaggta caccttgacg gatcatttca tctaaaagga tgtttgcgat acctacacca
    27481 gcagtaccag caccaaatgt caagatcgtt tgatctttca attgctcacc tgatatattc
    27541 aatccaccta atacaccagc tagtacaacg attcctgtgc cttggatatc atcattgaaa
    27601 gtagtgattt tatcttcgta tttttctaag atcgttgcag catttcctcg tccaaaatct
    27661 tcccaatgta ataacaattc tgggaataat tcagtagctg attgaacgaa ttgatcaatg
    27721 aattcgtagt aagtttcgcc ttctacacga gcatgacgat tccctaagta taatggatta
    27781 ttcaataatt cttcgttatt agtaccagcg tcgattgata caggcaagac ttgtgcagga
    27841 ttgataccag ccgcggcagt gtagaccatc aatttaccga tagcgatgtc aacaccgttc
    27901 acaccccagt cacccatacc taagattcct tctgcatctg tcacaacgat caaacgaatg
    27961 tcgcgtccat ctgctgcgtt tttcaaactt gctttgatat tttctggttc gtcgattgaa
    28021 agaaatacag catcttgtgg tttcaagaaa atttcgttgt attgttcgat tgaatccgca
    28081 acgacaggat cataaacgac aggcatgaat tcaactaaat gttcgcccat caatttataa
    28141 aacaatgtcc ggttcgtatt gaatagattc attaagaaga tacgtttttc caaatcggtc
    28201 tgtttgctta agtattgacc gtaagcttgg acagattgtt gttccaatgt ttggacagta
    28261 cttggaagca taccagtgat tccgtatttt gcccgttctt cttttgtaaa agctgttcct
    28321 ttatttagaa aaggattatt taataagttt agccctgtaa gcatgaatga ttcctcctaa
    28381 gttttaatac aaacgaagta taggagtcag atgaggatat agtcaaaaag agcaggtata
    28441 ataaatttta tttatatgag taatagagtc aaaagaaatc aaaatagcgg tatttgttct
    28501 ttttatatca acaatccttg cttgattact atgcgtatct cttatatgct aaagaaagaa
    28561 aagtagaaga ataaaggata tgttagatag ataaatagaa tcctgaataa ttcatagctt
    28621 taattctttg gcacatttta ttgaaatatg tatcactaat atccctatca actgattttg
    28681 actaaaaagt tacttcaaac attcactgtt caaaaatatt gaggaataaa ttttgggtgt
    28741 aaggcatttt gagcatactt ctccctggat aacttcccag taaaaaaatc gttagattgt
    28801 tggattagat ccattgactg gctcgcctca ggcgagcaaa gaccctgtag aattcattct
    28861 gatacacatg ataactagat aatttgagat agactcgtct acccgtttgg actaatttcc
    28921 cagcaacttt cagaaacttc aatcgaatag aatgaatggt cattcccttt tggacttctt
    28981 caaagccaat cgttcttaag aagttgacta agttgtaagc tatcaagctg agcatcattc
    29041 ggacatgatt ctccaaaaag cgaggactgt ctgttttgtc aaagtaaaag ccagctttcg
    29101 cttctttaat gaagttctcc attgtgccac gtttggcata gatagaaaag atggtatcag
    29161 gagaaacatt ttctgataga ttcgtcacga taaattcatg tcgaaagagt agttcgcccg
    29221 cttcacgtgt tgagcgtata cacacgcgac gactttgtga ccaggattgt gcttgataag
    29281 tggtcgagaa gtactgaact tctcgttctt cccacttttg attatcgcca taaagtactg
    29341 atttctcagc tatttgacct agtctacgat tattcttcag tcgaataaca taatgactct
    29401 tttttgattc acacgaatca tacacaccag gtgtagcgaa cccgctgtct ccacgaacca
    29461 agatgtcagt gtttggtaga gagtgattat agtgctctaa taaaggtgtg agaaactcct
    29521 ttacgccttt tgatgtatat tgatttcctg aacgtagttc agcttttaag aagtcaccag
    29581 tcaatccgtc aaaagctacc aatggatggt aaccataggt ttggtagtgt gtattataat
    29641 tcgtttgttc ttggtgacca aatgtatctg aatgggtcga atctaaatca atgattaatt
    29701 ccgtatcatt acggatgagg cgtgctttat cgataagttc ttggttcaaa gcttgaagtt
    29761 catggatatt ctcctcagat agtcgatcta aaaatcggga aagtgaagat tgagaagcga
    29821 gttctttcct atctaaaaca gctttaaaca caggatcttg tctcaggaga ttagctgcag
    29881 aatcagctga gtaaccagca attaattgca taatgaactg ctcaagtatg gataagttgt
    29941 catgagtaaa atacgctcgt tcgtcttcaa tgtgaatgtg ttgcttagcc agttcagaaa
    30001 aaccaaagat gttcatcagc tctttcacta ggacgagacc cgaatcactc gataattgac
    30061 cacctgtatg agaaatagtg atatttgaat tgaattttac ttggtttttg tgtaagctag
    30121 tcatgaaaag aactcctttc ttttggttgg tttagtcact ttaaccatag cagaaagggg
    30181 ttcttttttc atcacttaac gggtgaagat gaaaaagtaa ttgtaagctg ccgtgaggca
    30241 gtttcacatg gttttaatta aaagtatgaa ttattcaggt agaatctctg aagaataaat
    30301 cacgtgatcg tgacgataaa atacacttac gtcaggtgat aaaaaataca taagtagaaa
    30361 actaagagga ggaacagaaa atgaaagaag ttcaattgat cagaaaaagc gaactatcag
    30421 aaggtggttg caatgcatgc ggcgtagtag aagcaacaag ctatacatta aagcttggtg
    30481 ccaacaaagc gataatctct gagttgacag taggcggtct tgtggacagt ctggcgcttg
    30541 ctgaaggttt tattggagaa gatatttatg agatgttttc agaaatccgt cagttaaaaa
    30601 aaggggaaaa ctgcatagag gttcatcatg agagcccgaa tgtgagattc aaacgtggtg
    30661 ataatgagat gatcttcaat aatcatgtgt ccgatcacac cgagctatat ggaatcgtga
    30721 atcaaatact aactgagctt tttggattgg gaccttatgc gttcaaggaa gaaaacggta
    30781 atccaaaatt aaacgaagaa tggcaagaaa cgattgaaat acaaagaaac aacccgcact
    30841 tatttcaata ggcatccaca gtatttcgta aaagggactt tgacacgact tttgtcaaaa
    30901 tcccatcgtt ttttatctat ttgtgttcta aacatttctt tcagagtagt aacaactgga
    30961 atgactgaaa tgtaaacaga ttatttgcct gtatcaatac ggaagagtaa tataaagtgt
    31021 gtaaacgcta tcatattatt cattcaattt tttatagagg gaaggcattg aaatggaaaa
    31081 aacgtatcaa gcaggtacaa atgaaggaat agtggatttt atcaatatgg aagatttaga
    31141 aatagccgct tcacaagtga ttccagctgg tggttatggt tacatcagca gtggtgctgg
    31201 cgacttattt acttatcaag aaaatgaacg ggcattcaat catcagctga ttattcctca
    31261 tgtattaaga gatgtagaat tacccgatac aactacacat tttgacaaag aaacgctgac
    31321 tgcaccaatc atcatggcac cagttgctgc tcatggctta gctcatgtga aggcggagaa
    31381 agcttctgca aaaggtgtag cagattttgg gacgatctat acagcaagtt cttacgcttc
    31441 ttgtacatta gaagagataa gagaagcagg cggggagaaa gcaccgcaat ggtttcaatt
    31501 ttatatgagc aaagatgatg ggatcaattt ggatattctt gaagtggcca agcgtaatgg
    31561 agcaaaagca atcgtattga cagcggatgc aacagttggc ggaaaccgtg aaacagaccg
    31621 cagaaatggg tttaccttcc cactgccaat gccgatcgtt caggcttatc aatcaggagt
    31681 aggccaaacg atggatgctg tgtacaaatc ttccaaacag aagcttagtc caaaagatgt
    31741 agaatttatt gctgctcatt ctgatttgcc agtctatgtg aaaggtgtcc agtcagaaga
    31801 agatgtctat cgttcattag aatctggtgc tggcggaatc tgggtatcta atcatggtgg
    31861 ccgtcaacta gatggtggtc cagcagcgtt tgattcgttg caatatgtgg cagaagcagt
    31921 agataaacgt gtaccaatcg tgtttgacag cggtgtacga cgcggacagc atgttttcaa
    31981 agcaatcgca tcaggtgcag atcttgttgc tattggcaga cccgttattt atggattgtc
    32041 tttaggagga agtacaggag tccatcaagt gtttgatttc tttaaaacag aattggaaat
    32101 ggtcatgcaa ttagctggaa cacaaacagt agaagatatc aaaaaaataa aattgcgtga
    32161 gaatcgtttt atttaagaaa caaatgacga aaaaaatagg agtgcggcag atgatgccgt
    32221 actcctattt ttatagttca aggatgtcac taatagaata atttgaaacg aaaaaataaa
    32281 attgaaatat tttttcatac ttcgccgatt gtaaaattaa gctagacaac taaaaaaagt
    32341 catttgtgct acactcaaaa tagttccgac gaaagaatta taaggagtga gtacaaatga
    32401 cctataacca tcttacacca actgaactcg taatgataga agcttatttc aatcaatcac
    32461 aacctgtttc aaaagtagcc aatctgttac aacgttctcg acaaactatt tataaggtct
    32521 atcgttttct taaatctggt ggaagcgcaa tgagctacta taaacaatat aagaaaaaca
    32581 aacgtaactg cggaagacat ccgattgttt tacctgatga gcaacaggaa tacattcaaa
    32641 aaaaggttgt tcaagggtgg gctcctgatg tccttattgg tcgagcagag tttcctattg
    32701 cttgttcggt acgcacactg tatcgaatgt ttaaagataa aacctttgac actcacgatt
    32761 taccaatgaa gggaaaaaga aaaccaaacg gtcataaaga aaaaagagga aaacaagggt
    32821 ttcgccggtc aatccgagac agaaagacag actataccca gtttgatcat gaatttggtc
    32881 acctggaagg tgacactatc gtgggtggaa aacataaaag cgcggtcatc acattagttg
    32941 aacggctatc taaagtcatc attaccttga aacccgaagg cagacaggct aaagacattg
    33001 aaaataggct gaatcaatgg tttcgttgtg tcccaaaaaa cttatttaaa tccatcacat
    33061 ttgattgtgg aaaagaattc tcaaattgga aaaagattag taataccaac gatatttcaa
    33121 tttattttgc ggatccagga acaccttctc aacgaggatt gaatgaacat tcaaatggct
    33181 tattacgtaa agatggtttg tataaagcta tggattttaa tggtgtatct gaaaccttta
    33241 tccaatcggt ggcatcgaaa cgaaatcata ttcctagaaa atcattacac tatcggacac
    33301 caatggaagt atttttgagt tacgtcacaa aagatgattt gtctaactta atttgacaaa
    33361 cgaaacttaa taaaaaagga agaatattca tgtaaacgat tttattgttt ttttctcttt
    33421 tatacaaaaa gggtcataat ttcaagtaag cggatgaatt catgaggatt ttttgcatac
    33481 tgtctctact atacttaggt cactaagaaa aaggagtgat ttattatgaa aaagaacatg
    33541 attgggacat tattactttc aacacttctt ctaggcggct tggcaacacc agcttttgct
    33601 gagggtcaag cgacttcaaa aggagatatt acctttactg aaccaacaaa taccgtggag
    33661 cctcttaacc ctactgatcc ttcaaaacca gtagagccag cagatccgga aaatccagca
    33721 actggccaga caggtccttt aacactggat gtagttcccg aattgccatt tggtacacac
    33781 gagattgaaa gcggaacaaa aacgtatcaa gtagacgctt caaaaaacga tacaccttat
    33841 ctccaagtat cagatcgtcg aggtgtaggt gcagatggcc aagcacaagg gtggaacgtg
    33901 acagtttcgg tttcagattt tgtaaacggt tcacaagttt tgcaaggcgc tgaattagat
    33961 tttggaacat ccactgttaa aagtacgtca gataacgaat ccactggacc aactagtcaa
    34021 actgtaactg ggttgagtaa agcaagtgca gcgacaccaa tatttacagc agcaaaagat
    34081 caaggtctgg gaacatggct ttctgtttat gatccagcaa atatcacact gaaagtgccg
    34141 aaagcagcag ctggaacatt cacggctgat ttgacttgga acctcgtggc tggcccagta
    34201 gcatagagta tgaaataaat tcaacaaact gcggtgaaaa tactataggt gaaggatttt
    34261 ttaccgcagt tgttgttgaa aggatgtggt ggacaatgaa tcaaagtaaa cgactgtttg
    34321 tttcgttttt ttctatttta agtctatttt tcatttttcc gtcaatcagc caagcaaagg
    34381 attcagcagg agactttgga atcaaaccgg tatttccaga gaaccaaata gataaagcaa
    34441 tcggttattt tgatttatta gtggcaccgg aacaaaatca gacactagaa gtaattatca
    34501 gcaattcttc agatgaagaa cgaacatttg aggtctctgt caatccggcg gtaacgagtg
    34561 atggtggaac gatcgattat agtcaaaaaa acccaacgtt agatgagaca ttgccgtttg
    34621 atgtccgtga cgttttgctc attgcgaaaa aggaaatcaa tgtatcagca catgcagaaa
    34681 caactgtacc tatagaagtg aaaatcccag caaaaagctt taagggacgt gtattagcgg
    34741 gtattcatgt ttcacctaaa gaagaagctg aaacagaaaa tgctacagaa ggagcacaaa
    34801 tcaaaaatcg tattgcttat aatctcgcag tcgttttaca agaaagtcaa gagacgatag
    34861 aaccggatct gaaactttta tcaggagatt tggacgaggt caacgcaaag ccaactgttc
    34921 agcttcgttt ccaaaatcct cagcctagga ttatcagcaa tttgatattt acgagcaaga
    34981 tcttttatga aaatcagctt tatattgaaa atacgtctaa tgcattttta gttgcaccaa
    35041 attcgaattt tcacttgaat cttgatttag ctggtgataa agcaaaagcc ggcgattacc
    35101 gcgcagaaat catagcaaaa tcaggcgaca gtaatgaatg gcgatttaca caaaacttta
    35161 cgatcaaaaa agaaaaagca caaaaagtaa atgaaaattc cgtttttgca gtacaagaac
    35221 aatctttccc atggctttat gcaggattgg ttgctgcagt tctagtgtta tggttgattt
    35281 tgctgttttt gtgggtccac aagaaaaaga aaaaaggaga aacaacatga tgaggtgatt
    35341 tcgttgaaaa catcgatctt attgggaggg attattgcaa gtttgctgtt cttcagctgg
    35401 caagagccag tccaagcaga taccaaaagt tcaggtacga ttacatttta tgatctaagt
    35461 ccaattccgc atccaagtgg ttcagtcaat ggcgcatcaa aacagcagac acagctaccg
    35521 atttataaag aatctaatca acatggcgta ttcccggaga cgaatgaaca aaagatccgt
    35581 tttctttatt tgatcattgg tatagaatta tggcttcttg cgtggttgtt gttatggaga
    35641 agaaaggaaa aggaggatga acatgttgct acaaaataaa ttttcatttt ttcgctcctt
    35701 cttttttact tcgctttttg ttttaagtat atttatcttt tcaccttcgg cagaagctgt
    35761 tggagcaacg cctcctgcca tttcttatga tccatacacg tatgaaaata tcgtacctcc
    35821 aaatggtgga cgtagtgcag tcaatggcgg gaagcttgtc caactaactg gggctaacca
    35881 attgcctaaa ttgactgcta cttatttgaa cgatggttca aatgatcctt ggaaaggtgg
    35941 cacggtccgt tttcattggg aaagccatgt gaaactaaca aatgtacttg atttggtgaa
    36001 tatcactgca caaggatttg catttgtggc gatcatcaat ttgccggaac atgtatcaag
    36061 cagtgatgta ttaaaagcaa ttgactggaa caacgcctac ttgtatattg ccggtgcccc
    36121 catcaaggtt actagcggaa gtttattatc agcaggaagc cataccttgc gttttgggat
    36181 ggggtcgtgg gatacaaata ctctattgac agatattatt gctattatta cgcgaggcga
    36241 attcaacgtg gacgatatcc cgcttcaatt tgatgttaca gctaatgtcg cgaagatgac
    36301 agaaaacgga gataaatatg atacgagtcc aaacaaaatt ttgaccaagg gtaaattaca
    36361 accttctatg tctaaaaagg ccgttttttc tgttgatttt tatgattcga ataatattat
    36421 ccaagatcgt atgttcaatt cgattttagc tccagctgca ggtagatttt atcctaaatt
    36481 aactggcgga aacttgaatc caggagcata tgcattttct gcaagtgcag agataaatac
    36541 gtggaatagt tacatctcac caagtgattc aacaggaaaa tataatacat ttgctgatac
    36601 cacagaatac gtggatggaa ataaccagaa ccttccaggc tctaccaata gtcggacatt
    36661 gacgatgggg ctgactgaag gacgttttga tcagttgcca tttagtcgct ttgatcgcgt
    36721 agtcaactat tttacaggaa aaaacgttac acaaggagca atactgactc atactccagc
    36781 aagcacaagt ggattgaatc agacagccaa tatcgtgtac agtgggaaag atgcgcaagg
    36841 cggattgctt tctcctgttg ctttaaaggt attggagcag tacaagctag acggaacggt
    36901 tttaccaaca aacttaacag ccgatagtca gtggggaaag ctcctttctt ctgcatcgta
    36961 ccaagtcacg gccaattggt cagcaactga actaaaaaag ggttcgatcc attacaggct
    37021 ttattcgaaa ggaacccaaa aattagcgac aggatggaca gatcaacttt tccaaacgat
    37081 ccaatcaaat aacgggggtg taaatacagc tacttctaca ctacctgcat tggcttcagg
    37141 gcaatattat tttgattatc gcttggtgga cgatgtactt actaacatgt atccgtcttt
    37201 tgcgtacaaa tggcagtctg aacaagctgg aatcaccact cttcctcaaa taacagtggc
    37261 taattttcca tctgtgagtg cagatagtca gctaaaaaac cttagcagac aacctgagac
    37321 aggagatccc ctgtctgctc tatcaggaga tacgattcaa gaaaatgtga cattcacgtt
    37381 aacaaaatct ggggatgcga taaccgacaa aaaaatcacg atctcacttc ctgaaaatac
    37441 tacttatatc aatggttcct taaaattaaa tggtacaact atcgcagata ccggcattca
    37501 acaaggagtt tctgtcccag cagatctatt gagcaaaata ggagatacca ttcatcttac
    37561 ttacaactat caattaaata ctgtagatac ttctttacaa tccgtcagca tactgacaaa
    37621 ggcagctgta ctttccagca atattacatt agcagatggt gctaagcttc ctaacccagt
    37681 tgtccaaaca tctgctaaaa caattcttgt ccccaaacaa gaattaacat tagttaatgt
    37741 accagatgat tttacatttg gtaacgatct ccccaaaccg ttgaagacaa gttattatga
    37801 atcaaaaggc gatttttctt ttgatgtgcg ggatactcgg cttccaagca cgtctccttg
    37861 gcaactgaca ggtacattga cttctttatt taagaatgat caaggtcaag aattaagcgg
    37921 aacaaaatta tacttcaatc attctggatc aaagcaattg attcagcaag gtcaaaacac
    37981 gttgatttat gagagtgatg gaacagctaa aggagaggtt ctcgttgact ttcccgatac
    38041 cgatggttta ctcttagagg taaatagcag tacgaatgct caacctggcg caacgtatca
    38101 aggcatggtc acatgggaac tgacagctgg gccaaccagc taagtgatta gaatttgtat
    38161 ggatctgtga taactgtcac agatcctctt tttcaaggat gagaaggaaa cttctgctgt
    38221 taaaatcaga aatgcttgaa ggagtggact tggaactttt cagtttgctt gaaaaaaatg
    38281 aacaacggca aatcaaactt ttccaacttt tattagagga aaaaggagag gtttccgtta
    38341 ataacgtact tacccgctta tcagttgatc gggctacctt gaaagaagat ctgttttatt
    38401 tacaagctaa cttggaaaat atcaaacaat tggaaattgt tatccaagaa gggaatattt
    38461 tattaatcag acatggaaat atagcagcag tcgatgttta ttatcattgt tacttgcgct
    38521 attctacaaa atatcagata cttctttatc tgttggaaaa tggctcattt gatcggcaga
    38581 aacttgtcat ggaacttaat atcagttcgg ctactttgtc tcgcagagtg aaggaattaa
    38641 atgattcttt aaatgagttt catctgaaaa taaaaagcgg gcggctgacc ggtccagaaa
    38701 gtcaaattcg ttatttttat ttccaattat tttggtttgg acgtccctat cttgaaaaca
    38761 aaaaagaata cagtgattta tcaggaaaca cttttttaga tctgctgcaa aaagagttta
    38821 gttttgctat tacagaagaa ggacgattga aatttgaaat atggctttcc attatgaaga
    38881 agcgaatgcg ctgtacgaaa aaatccaaac cagattatag caatcttcct gttggattgt
    38941 ttcaaacgac acaattttta ttggacctcc atacattttt gacccgttac ttctttcaat
    39001 atgcctttcc ttggaatcaa aatgaaacgt tggtttttta tcttttcatg atcagtaact
    39061 ttacattaga tacacataac tcgcatgtcc aggagttttt gataagcgaa aaatacaaag
    39121 acgacgtagt agctcaattg aatcgaacgt ttaaacaatc gatagaagaa tatttctctt
    39181 tttatgtttt tccagaagag atttcgcaaa agattgcgtt gacagtgatg caaaatcacg
    39241 ctcattttgc ctattttaga ggatggatga atatttttgg ccaagaagga ttaaaacagc
    39301 ggttaactga agtagaagat aaacaattcc tggttttatg tgaaagattg gtcagacaaa
    39361 catttgctat tgttgatttt actgaagtag acgaggaaaa tgcccgcttt gaattatttg
    39421 ctcgctatgc atctatttta cggacgctga tcaagcagtt agaattacgt ctgcaaattg
    39481 cttatgatct tccttatgaa aggctaatgg cggatatcgt gaaagaaatc atccaggaac
    39541 aattgctgaa tacgatcaaa tatgatttag tcgaatacga gaaagaaaag cagtatgata
    39601 tcattttgac tagccaattg aaagagtacc cgtcacagaa gtccgctaaa gtttttgtat
    39661 tcactagcaa cgaatataat tatgatttcc cctatttgaa ccaattttta aaagaatgtt
    39721 atttagaaaa attgaattta aggatgaata aaacatgaaa atcgtgatca ttggtgcttc
    39781 ccatgaaggc atcacagctg ccctaaatct aagaaaactt caaccgaaag cagaagtttt
    39841 actgatcgat gaagatcata aagatggact aggctatgtt tcaaatggaa tcaaccttta
    39901 tttaaaagga aagatacgta gtttagctga agttgcccat aacatgcgga ctcttcaaga
    39961 aagtggggca aaactgatca ccgaatggag agtaactgaa cttgatccag acaaacatca
    40021 gctcatcctc gcatccaaag aaggaaaaaa cgagaccata acctatgata aattaatcgt
    40081 agcgacaggt tcctctcctg tcaccttgta taaacaaatt gaagctgaaa atgtctacac
    40141 ctataaaaat ctagtgcaat ctaagcaagt acttgccgct ttaaaagaag caaaagaagt
    40201 agtgatcttc ggtgctggtt acattggatt ggaattagca gatgctttga gaaataaagg
    40261 atacattatt catctagttg attatatgcc gaatgtttta tcaagatatt tcgataagga
    40321 tatgatcaac agttttcaaa atcagctgca aactaagcaa ataaattttt atccaaatga
    40381 attcttgatc gattggaaaa aatcagaaga gaaagtagta tccgtacaac ttctgtctca
    40441 aacaataaag gcagatatgg tcattttttc tgcccaaact cgtcctaata cgacattgtt
    40501 aaaagaaaaa gtggctttgt atgaggatga gactgtgata gtcaatgaat atctgcagac
    40561 aagtgatccg gacatctatg cgattggaga tatcgtccca gtcagttttg acaaaaataa
    40621 acggcatctc tttttacctt tagttactcg agctgtacat atggcaagag cagtagcgct
    40681 aactttgagt ggtcagccaa ccgcttacga cctcagacaa aagataacag cagcagtcat
    40741 tatagactac tttttaggaa ctgtagggct gacagaagat gaagcgccat tcttggaaca
    40801 aaataccggt tcgtgttcag gagaatttga tctatttcca caatacgacg aagaaaacaa
    40861 aacagtaaat gcaaagctga tctaccatcc ggatacatta gaaataattg gcggacagct
    40921 gataagccaa gaattcttat tatcagattt gaacctgctt gcggatatcg tgaaacacaa
    40981 aactacgatt ccacagctag cagttgagga ttttggattt ctgtctgaat acacctctcg
    41041 ttttcactac ctcaatgagt tagcttttaa agtactagct aagagagcaa atagcaatag
    41101 agtagataaa agactgtgag aaatatcacg gtcttttatt gtgtggggat ataataaata
    41161 ttaatcaatc gagaataaaa aattcatata atgaaatagc ggaagaattt tttgagcata
    41221 atattcgcca aaaatttatc aagtgataaa attgttgtat aaaatgatta tttcaggagg
    41281 aggagttaaa tgtttttagc attaaatgaa attatgcatt caaagttacg ttatgcgtta
    41341 gttgcagggg tgatgttttt gatcgcctac ctggtatttt ttttgactgg gttagcttat
    41401 ggcttagctc aagataatcg aacagcagtc gacaagtggg aggcagattc gattgtgctt
    41461 tcgaaagatg caaattcaaa tctagggatg tccatgatta ctaagaagat agcagaagaa
    41521 gtcgaaggtg ggaaagtggc gtatcttgcc caaacacctg gagtcgtgac tagtaaagac
    41581 agtacagaag aaggaaaaat caatgttagc ttcttcggta tcgataaaaa tcaattcatc
    41641 atgcccaatc ttgttgaagg aaaagctttt gataacgatg atgaggcagt aggcgatatt
    41701 agtttgaaag aagaatatgg tctagccata ggagatacag ttaagttatc tggtagtgac
    41761 aagacgttta aactaactgg ctttacggat catgccaaat tcaatgtctc accagttctc
    41821 tatacaacca tcaatgctta ccaagaaatc cgcttcgaaa aagaagatac aagtgaaaat
    41881 gcacggatta acgcaatagt tgtcagaggt aaaatcagta atttacctga agatttagaa
    41941 cagatcaaaa tttctaaatt catcaatgaa ttacctggat ataatgcgca agtattgact
    42001 tttggattta tgatcggatt tttgatcgtt attgcggcta ttgtgatcgg gattttcatt
    42061 tatgtactga cgatgcaaaa gattaatatc tttggtgtaa tgaaagcaca agggatcaca
    42121 ggaggattta ttgcgcgttc tgtagtggca caaacattca ttttatcatt tgttggtatt
    42181 cttcttggtc tattaggaac agtgggaaca tcccttgttc tacctgatgc ggttccattc
    42241 caaagcaatt ggctgttttt tggcgtcatt agcttgttga tgttagtagt agctgtgtta
    42301 ggcgcactat tttctgttcg gacaatcgta aaaatcgatc cgttgaaggc aattggataa
    42361 gaaggaggaa gaacatgaaa gcaatagaat ttaaagaagt agaaaaaagt ttccaagatg
    42421 gcgaccaagt agttaaagca ttgaaagaaa caaccatgag tatcgataaa ggggaatttg
    42481 ttgcaatcat cggtccttct ggttcaggta aaagtacttt tttgactttg gcaggcggat
    42541 tgcagacgcc tagcagtggc tctgtattta tcaatgatca agaattcagt aatcaaaaag
    42601 aaagcgaacg cgttaaacta cgttttaaag aaattggctt tattttgcaa gcttccaatc
    42661 tggttccttt tctgacagta aaaaagcagt tattattagt agataagatt aaacatgaac
    42721 atcgtcaaaa agaagcaaac gaattatttg agcagcttgg cgtggataag ttgatcaaca
    42781 agtatccaga agaactatct ggtggagaac ggcaaagggt cgcaatcgct cgtgcattgt
    42841 acaatgatcc gtccattatt ctagcagatg aaccaactgc tagtttggat tcagaaaagg
    42901 cacgagaagt agtcgatatc ttggcaaaag aatcaaaaga caaaaataaa gcaacgatca
    42961 tggttactca cgatacacgg ctgattgaca aatgcgatag aatatttatg atggaagatg
    43021 gtgtattgaa agaaaaagaa taaccaaaaa gcaggtggaa aaatggtcca tcgatacaac
    43081 aaagatacac agcaaaaaat tttgaaagaa gcgactgact tgtttatgtc caagggatat
    43141 ctcggcactt caacaagaga aatcgcacaa aaagttggga tcacccagcc caatctctat
    43201 cattatttcg gtgataaaga aaaactatat acggcagttt tagaaaacca cttaaaagat
    43261 gttggaaaag ctttgagaga aatcgttcaa acaagcgaaa cgggatttca agtaaccttg
    43321 acaaaaatgg cgcagttttt gattgagaca catctggtcg atttgtttct gatgcttcac
    43381 gatcttgaaa gcaatctttc aaaggaaaca agagatcatc ttttctttct atggaagaaa
    43441 aattatcgtg aaccttttga agaaatcttt tcagcaaacc aatctgtgct aagaaatggg
    43501 atttcaaaag aaatcgcagc aaggcatttt ttcttagtgt tagcacctta catcactcgg
    43561 tcgaaaagac ctgtggaaga accgctaaat gtggcgcaat tgatcgattt atatttgaat
    43621 ggtgtattag gagaagcgta aagctgttat atagggagaa aatagttagg tgaagaagca
    43681 agatatgacg aagatcgtta tatcttgctt ctttatatag gtagtttttc ataaatacaa
    43741 atttgttatt ctctcatgat tatcagggta tatttttatt tattatgtcg gtgttccagg
    43801 aagtgtttta atttaataac gaagaaatta attgtttgta gaatgaagga aactatttat
    43861 tgggtagatg aatcaccacg aatagtcaaa aggagatttc aggatatgcc aaaaatgaaa
    43921 aaaatcattt tagcagtaat gttattttct acggttatat cttttacagc cccttttttg
    43981 cttacttaag ctgattttaa gttttggccc ttagtctgga tctacgaggt gagagtcaag
    44041 aatgaaaaaa ataattggag cttttgtatt tgcaatcatt atcattatta tctgttttca
    44101 aatagatgtt tttggtcaaa aaaagatagt tggacaaacg gtcagcagta cgaacagtac
    44161 ggttacaaag acaaaggaat catttgatga tacagaacaa gagacattaa tcaaaatcgg
    44221 aaaaaatgac cgtacactat atacagatga ctcgctaact accccacttg caactataaa
    44281 cggcggagag ctggcggagt ttctttctga aacaaaaaat gcttatcaga tcaagacaaa
    44341 tgatggctat actggatatt tagccctcga agatggaacc aaagtcacaa agaatataca
    44401 aacaaaaccg gaagagctat ctgatgcagt gattgtatta gatcctgggc atggcggaaa
    44461 tgatactggg gcattaagca atgatgaaca aacagaagaa aaagatataa cattaagtac
    44521 agccataaaa gtaaaaaagg cgctagagga tgctggagca acggtttatt tgacccatac
    44581 tacagatgaa ttagtacagc ttggagatat atgcgactac agtgaagaaa aaaaggcaga
    44641 tgtctttatc agtttacacg cagactctac ggaatatgcg aatgaagcaa ctggaatcac
    44701 gacatattat tactatggac aagaggaaac attagcacag acaattgctg actccttcac
    44761 tgatctgcct ctggattcaa gaggaatctc cactgggaac tatcaagttc ttcgagaaaa
    44821 tcttcaacct tctattttaa tcgagatggg ttatatgaat aatgatagtg atttaaagga
    44881 attagtaaca gacagttatc agcagcagat tgctgcaagt ttgacacaag cattgaccac
    44941 ttattttcag taataaataa gcccagccaa acaaaaatag ctcctcatat ttttcgtttg
    45001 gccgggctta ttatcgcaga tgcaagcttt taaacaccgt ttaaggaaga ggtcgtgagc
    45061 ctgccgttca actctgagtc acaaggagca atttcaaaaa acagcttctc atgtttgatg
    45121 actattgtga cttgtgaccg aggagttggc atgagaacac cgtttaagaa aaaggttgtg
    45181 aaatcaacgt tttgttctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    45241 gccgtttgtt gagctaatac cgaggatcag atgattgaac accgtttatt cggaacaagg
    45301 gactgtgaca tgacttttgt cacagttcca ttattttttt agtagcttca aagctttttc
    45361 tgtattggca aaatgaagtt tggcatattt ttcaagcata ggcatgccgc ctttctccaa
    45421 tagttttgcc gctacttggt cagatttcgt tccagctcct gacggaaggg tgattccagc
    45481 ttcagctgat gctttgtcta actcttccaa aaagctggca cgactgatga tagaagcagc
    45541 agcaacagct aaatgatatt gttctccttt agtaacgaag tacagttttt cttgtaattg
    45601 atttttttct aaacgcgcat attttttaaa atttgcttcc ggcgtaaact gatcgatcaa
    45661 aatcgcttct ggtttttctg gctcgatttt ttttagcagc aaatggatcg cttgattgtg
    45721 caaagcgact ttcatccgaa cggcattgta ttttggttgg atctcattgt atttttctgg
    45781 agataagaca agcagtttat aggggatggt tttttttaag atgtcagcaa gctggatgat
    45841 ttggctatct tttaactctt ttgaatctct aacaccaagt ttacgtaatt ctgcaagctg
    45901 atcacgtttc acataagcag cacatactgt tagcggaccg aaatagctgc catttccgac
    45961 ctcatcacta cctaaaacgg ataattgact gatatttttt ggcaaagaag aggctgtttt
    46021 tgctggcttg gcttgtttat cctgtgcctc cccccattta tcagcttcta ttgctgcatt
    46081 cgtaccttga aacatcactt ttccggatgt ataagcggta atggtcgtcc cgtctttttt
    46141 tgcagcaaag ctagtataag gaatctgttt atttaacaaa taagagcgat agtatgcttt
    46201 catttgacta agttggtcgt ttgttgcttt gataatgacg ttgctcataa aattctcctc
    46261 gtttttatac ataaaactat accgcaactc acgacttttc gttttctaag agattgcgag
    46321 acatagcatt tttttaaagt ttatcatata atagttaagt atgtgaaaaa tatttgtgga
    46381 gaacggtgaa agaaccaact tcatggtaca ctaaataaaa ctttcatcaa ataggaggaa
    46441 atgattcgta tggcgcacga aaaaacacgc tacaaagcgg tgattgccaa ccaaacatat
    46501 actattatcg gacgggaaac caagcatcat atggatatcg taacgaagct gatcaatgaa
    46561 cagttagcag aattgaaaca attatcacca caaatggata atgaacaagc agcaatcttg
    46621 atggctgtca atgcgctttc ggatcaattg aagaaacaag aaagaatatt ggagctggaa
    46681 gaagaaacag cagaattaaa gaaaaaaatg atcaaattta cagagcttga aaatcgtgtg
    46741 aaacgcattg aagcaatcga gaacgaagct cgggaagtat tgaaagaaaa tggtcaagca
    46801 gatcatacca ttaaaaatca tgtggaagca caacagattt taaacgagaa acgaaaagga
    46861 caaattaaac aaaaatcttc taattaagat ccttttatca aaataagaaa aatttaaaaa
    46921 agttagaaga gaaaggaacg tagcatgctg agtttgctga ttttattttt attgctaatt
    46981 gcgttttttt ctggagcacg tcgcgggttt gctttgcaaa tcatattcgc gattggatat
    47041 gtattgtcat ttattgctgc ccagcatttt tacaaaccat tagcaagtca tttggaatta
    47101 tacattccat acccagctgt caccccaaca tcaaagttag cattttttga tcaggtattt
    47161 gcctttcacc tagatgaagc tttttatgca ggaactgctt ttctgattat tttgctaatt
    47221 ggctggctgc ttacccgatt tgtgggggtt tttgtccatg gattgacgta tgtgccgatc
    47281 ttgagacagg tagactggat tgcaggaggc atcctgtcac taatcatggc ttacgttact
    47341 atatttttga ttttgcagtt acttgccttt gttccattag atgtggtcca aaatcaattc
    47401 ggcgagcata gtttcgctcg ttttatcgtt gagagaacgc catttcttac caacaagatg
    47461 tatgagcttt gggtaacgaa tattttgaat taaggaaaat agtgctggaa aatatttcgt
    47521 tttttgtaca gagtagttta tttaagaagg ctgcgtagcg tatgtccggc acagctcttt
    47581 tttcatagtg aaagataagg tgataaaatg aataaccgaa tccttgaaac attggaattt
    47641 gaaaaagtca aacagatggt cagacaattt gtagtgactg cacaaggaaa agaagaactg
    47701 gcagaattgg taccagtgag tgagaaacaa acgatcacaa actggcttca agaaactgag
    47761 gatggattga aagtgcaacg tctgcgaggc gggatcccta tacctaaaat tgaaaatatc
    47821 cgtccccata tgaaacggat tgagatcgga gcagacttga atggagtgga gcttgcacaa
    47881 gtttccagag tattgtctac gacaagcgaa ttgaatcgtt tcattgatga tctttctgac
    47941 agtgaaatcg aatttgcacg tttgtatatg tgggcagatc agttagtcac gatccctgtt
    48001 ttgagccgcc gtttaaagga agcaatcgat gaagacggac gcgtgactga tgacgcttct
    48061 cctgagttaa agagcataag acagaacatt cggcgaagtg aacaagctgt tcgtgaacag
    48121 ttagatggga tcgtccgtgg gaaaaatgca aagtatttaa gtgatgcgat catcaccatg
    48181 cgaaatgacc gctacgttat cccagtcaaa caagaatacc gcggtgtatt tggcggtgta
    48241 gtccacgatc aaagtgcttc aggtcagact ttatttattg aaccaaaaca agtggtggat
    48301 ttgaataatc gtttgcgtca ataccaaatc gctgaacgaa atgagattca acgtatttta
    48361 agtgaacttt ctgctgaact tgttccccat cgtcaagaaa tcatccacaa tacttatgtg
    48421 attggtaaaa tggatctgat gaatgcaaaa gcacgatttg gtaaagaagt aaaagccatc
    48481 gtgccgggaa tcagtgaaga caatcatgtc gtcttgaaac aagcaagaca tccactgatc
    48541 gatcaggaaa aagtagtttc taatgacatc acaatcggaa aagactatca agccatcgtc
    48601 attacaggac ctaacacagg tgggaaaacg atcacgttga agactttagg tctgttacaa
    48661 ctgatggggc aagcaggatt accgattcct gcaggtgaag aaagtcaaat cggtattttt
    48721 gaggaagtat ttgcggatat aggagacgaa cagtcgattg agcaaagtct atcgaccttt
    48781 tcttctcata tgacgaacac tgtagatatt ttatcaaaag tcaatgaaaa aagtctggta
    48841 ttatttgacg agctcggtgc aggaacagat ccgcaagaag gtgctgctct agcaatcgca
    48901 atcttagatg atcttggtaa aaagtctgct tatgtgatgg caacaacgca ttatcctgaa
    48961 ttgaaagttt atggatataa tcgggcaaat acgattaatg ccagcatgga gtttgatgtg
    49021 gatacattaa gtccaacgta tcgtttgctg attggcgttc ctggacgaag caatgcgttt
    49081 gaaatctcta gccgattagg tcttgataca gaagttattg atgaagcgaa gcagttgatg
    49141 aatgatgaga gccaagattt aaacgaaatg atcacagatt tagagaatcg ccggaaaatg
    49201 gcagagaccg aatatctgga aatgcgtcat tttgtttctg aggcacaaga acttcacgat
    49261 gatttgaaag aagcttacag ctatttcttt gaagaacgtg aaaaagaaat ggaaaaagca
    49321 aagaaaaaag caaatgaagt cgtgtcagag gcggaagaaa aagcagaaaa aatcattgct
    49381 gatattcgta aaatgcaaca gcagattggg caaggaaatg tgaaagagca tcagttgatc
    49441 gatgcaaaga cccaattagc aaatctacat caagaagaaa ctttgaagaa aaataaagta
    49501 ttgaagaagg cgaaagaaca aaaaacgttg aaaccgggag acgaagtgtt ggttacgact
    49561 tacggccaaa gagggacgct tttacgtaaa aacgggaatc agtggcaagt agaaatcggt
    49621 atattaaaaa tgaatgtgtc cgaagacgag ctgacgcctg ttgctcctca aaaagagccg
    49681 acacaacgag ttatccatgc tgtacgatct gaatctagta gtcacgtgcc gaatcaattg
    49741 gaccttcgag gaaaacgata cgaagaagcg ctaagtgagg tagatcaata tttggattct
    49801 gccattctag ctggttatcc tcaagtgact attgtccacg gaaaaggaac aggcgcactt
    49861 cgcaagggaa ttactgatta tcttaaaaac catcggagcg tcaaaagctt tgagtttgca
    49921 ccggcaaacc aaggcggaaa tggtgcgaca attgttaaat ttaagtaaag cttactaaag
    49981 gtaagcagat ggttcgctat ccaagtatag aaatgctata ctagtcatag attttaaaaa
    50041 tggaggtctt tgatatgtca caagtaatta cagataagac atttaatgaa gaaacagata
    50101 aaggacttgt attgattgat ttttgggcaa cttggtgtgg tccatgtcgt atgcaagcac
    50161 cgattttgga tcagttggaa caagaatatg atgaagaaga attcagaatt gctaaaatgg
    50221 atgtggatga aaatccagaa actcctcaac aattcggtat catgagcatt ccaacattaa
    50281 tgttgaaaaa agatggtcaa gtagtagaaa aagctgttgg tgttcattca aaagaacaac
    50341 tacgtcaaat gatcgatcaa tatttataaa aacttacagc aaaaataagc tgagggtggg
    50401 actgaatcca gttccgtcct cggttttttt gcggttgagt taaggttatt caaattaata
    50461 ttttttgagt ataattacgt tgtcaggctg gattgttttt ttgacagaca tgctataata
    50521 actttaattg aatatgaagg ggagtaccag atgaacgaac gaatcaaaaa caaattagct
    50581 ttacttcctg atcagcccgg ctgttacttg atgaaagata aaaatggaac gatcatttat
    50641 gtcggcaaag ccaaaatatt aaaaaatcgt gtccgctcat attttactgg tagccataat
    50701 acgaaaacgg aacggcttgt tagtgaaatc gaagactttg aatacatcgt tacagaatcg
    50761 aatatcgaag cattgctctt agagatcaat ctgataaaga aaaatgatcc taaatacaac
    50821 attatgttaa aggatgataa aacctatcct ttccttaaga taacgaatga aaaatatccc
    50881 cgtttggtga ttactcgtaa ggtactaaaa gacaaagcac tgtattttgg accatatccc
    50941 gatgtcggtg cggcgaatga gacaaagaag atcctcgatc gattatttcc tctgagaaaa
    51001 tgcaaaccta gtcagacgaa ggagccttgt ctttactatc atttgggaca atgcctgtgt
    51061 ccttattatt tcgacgtaga tccgaaagtc tatacgaaaa tcgttgatga agtgaaaaac
    51121 tttttaaacg gaggacacga aaaaatcgag tcagagatcc atcagaaaat ggaaaaagct
    51181 gctgaaaaca tggagtttga aaaagctgca gaataccgag accagatccg tgccattgag
    51241 acaatcatga ctcgccaaaa aatgacaaat actgatttac tggatcgaga tgtatttggt
    51301 tatgcgattg ataaaggatg gatgtgtgtc caagtgtttt tcgtaagaca aggaaagcta
    51361 attgaacgag atgtttcaat gtttcctttc tataacgaag cagaagaaga tttcttgact
    51421 tacctcggtc agttttatca ggaaaatgag catttcattc ctaaagaagt cttgattcct
    51481 gatacgatcg acaagcccag tgtagaagct ttgttagcta cgaaagtgct gcaaccaaaa
    51541 cgaggagaaa agaaaaaatt agttgaattg gcgagtaaga atgcctcagt tgctttgaat
    51601 gaacggtttg atctgattgc cagaaaacag gaacggacga tcggagcagt tgaacgttta
    51661 ggaaacgcga tgaatatccc gaccccaata cgtatcgagt cttttgataa ctctaatatc
    51721 atggggacga atcctgtggc tgcgatggtc gtattcattg atggaaaacc ggcaagaaaa
    51781 gaatatcgga aatataaaat aaaaacagtc aacggaccgg atgattatgc atcaatgaga
    51841 gaagtcatct atcgcagata ttcgcgagtg attcgcgaag ggttgccttt acctgatttg
    51901 atcttgatcg atggaggaaa aggacaagta gatgtggcaa aagatgtctt agaaaatcaa
    51961 ttaggaatcg atattcctat tgctggcatg gtaaaaaacg ataaacataa aacaaatgag
    52021 ttgctattcg gtacagattt ggctgtagta ccgcttgagc gaaattcacc agagtttttc
    52081 ttactccagc gaatccaaga tgaagtccat cgattcgcaa tcactttcca tcggcagcta
    52141 cgcagtaaaa acagttttgc ttcccgatta gatgaaattg aagggttagg accaaaacga
    52201 aaaaaaatgc tgcttaaaga gttcaaatcg ttgaaaaata tcacagcagc cacgacagaa
    52261 gaattacaag aaatcggact gccgaaaaat gtcgcacaaa acgtatttga taaattacat
    52321 caggaatgag gaaagggcac tttacaaaga aagtaaagtg tcctatcatt ttatgtacaa
    52381 caaaaagagc gtttacgttt tgttggtaaa taataaatcg cttacgtctg gtgtctaagg
    52441 atatatgtga tttcttgaag aaggagtgaa gaaatgttag aagtcaagga tcttgttaaa
    52501 acttttggga actacacagc tgtcgatcat gtctctttcc aaatccccga tggaaaaatc
    52561 atggggctaa ttggccaaaa cggggctggg aagacaacga cttttcgttt gatcttggat
    52621 tttttgacac aagaccaagg agaagtgtta tggaatggcc atcatcttgg ggagaaagat
    52681 tatgatgtca tcggttatct tccggaggaa cggggacttt atccgaaagt aacgattcaa
    52741 gaccaactgc tttattttgc agaacttaga gggaaatcaa gaaaagagat tgaaccaaaa
    52801 atcgactttt ggatggaaaa atttcaggta aaagggaaaa agacggataa agtaaaatct
    52861 ttgtctaaag gaaatcagca aaaagtccag ctgatcgcca cgttaatcca tgaacctaaa
    52921 ttgatcattt tggatgagcc attcagcggg ttagatccgg taaatgctga attgctaaaa
    52981 gatggaatat tggaattgaa aaaacatggt tcatgtgtca ttttttctag ccacaatatg
    53041 gataatgtag aaaaaatctg cgatcatttg atcatgttgc gaaacggaaa aatggttttg
    53101 aatggaaaag tacatgagat tcgtgaatca tttggccgta cgaagttatt cttggaatct
    53161 ggtttaagcc aacaggaagt agaagaaatc gaaggcgtga aaaaagtgat ccttcatgaa
    53221 gacaatacgt tagagattac cttagaagat cctgaagctg ggaaagaaat ctttactcgt
    53281 gcgactcagt tcggctacat tcctatgttt aaccagcaac cgccaacttt ggaagagatt
    53341 ttcaaattga aagcaggtga aatcgatgcg taagttttgg attattacca aagatgtcta
    53401 tttaaaaaat gttaagtcga tttctttctt gatcatgata ttagtccctt ttatcatgct
    53461 gggtatcatt tatgtagcag gaaactttgc tcaacaaaat agtgatgtca ataaaatcgg
    53521 cgtattggct gataatgagc aaatcagtca gcaaatgagc caagtcaaaa cagatgattt
    53581 tgagttcaaa gcagttgatt ctgaagaaaa agcacaaaaa gagctttcgg aagaaaaaat
    53641 cgatgcgtat ttagtagtct caactgagaa tgataagata aatgggatgc tttatagcga
    53701 aaattcatta ggacaaagta cacaactact gatacaacaa caactgactg gcttgcaatc
    53761 gatgcttcga gcgagtcaat taggcatttc accagaagaa gtagctagct tgagccaaca
    53821 agcaagtttg actcgacaaa aagtcaattt tgattcaaaa ggaaaaatga cagtcggaga
    53881 agataactcg acagtccaat acgtcgtaag ttatgtagca acgattgtct tgttcattat
    53941 tattttgacg tatgcgcaga tcattgcaca agagatcgct tctgaaaaag gcacgcggat
    54001 catggaagtg atcctgtcca gtacgactgc acaaaagcat ttttacggaa aattaacagg
    54061 tgttttactt gttgcattaa cgcaaatggc attatacggt attatttttg gtataggata
    54121 cagtcaattc aaagatatgg aaatggtcaa aaatatatta gacggtatct cattggataa
    54181 cttgattgga ccattcttgt ggtattctct tctattcatg atctttggca ttttgatttt
    54241 ttctgtttta gcagctttat gtggctcttt ggtcaacaaa gcagaagata cagcaaaagc
    54301 aattttgcca gtgacttatc tatctctagg cggatacatg cttggattga tactaggagc
    54361 ttcagatcca aacaatatcg ttattcggat cacttcgtat attccattct tatcttcata
    54421 tattatgccg attcgcttag caaatgagac agtcggtatg ggtggagcat tgatttcctt
    54481 gttggtactt gtagccgtaa cattcttatt gatgatcttg tctgcaaaca tgtacaaatc
    54541 gaatgtactc gtttatagcg aaggcggatt atggagttca ttgaaacaat cgatttcaat
    54601 tatgaggaat gaacgaaaaa aagcataaaa caatcctaaa atacagaagg aagctggaca
    54661 tctattgttc ccagcttctt tttgtataga cctattttat ttattttact atattggata
    54721 tatattcatt gaaaatagtg tataacaact ttataatatt gagtatacca atttttggag
    54781 gcgttttaat ggaccgttat ttagatttac tttcagaaaa attcccctca acggaggatg
    54841 tcatcacgga aatcatcaat ctcgaagcaa ttttgcagct tccaaaagga acagagttat
    54901 ttttgagtga tatacatggc gaatttcctg cttttgatca tattctgcgt atcggttcag
    54961 gaaatcttaa agaaaagatc aaagaccttt tttctgaaca aatgacggaa acggatatca
    55021 gccaattcac tatttttacg gcttatccag aatatgcatt aacgactgat tggtacaaag
    55081 aacaagataa atcgcagctg atccatcatt tgatcgatct tttacggttt acgacagtca
    55141 aatatacccg gtcaaaagtt cgaaaaggat tgccgaaaga atatagctat atcattgagg
    55201 aactcttgta tatcgatgac cgtatcaatg gaaaaaaaga ctatgtaaaa aaaatcattg
    55261 aacaacttgt tttgatgaag gaagaagaaa aatttttaac gaaacttgca cagacgatcc
    55321 aaaaatctgt catcgatcat ttgcatatcg ttggagatat ttttgaccga ggaactcaag
    55381 cagccaaagt gatggatcga atcatggatt tttcttctgt agatatccaa tggggaaatc
    55441 atgatatttt gtggattggt gcgtattgcg gctctgaggc atgtttactg acacttttac
    55501 ggattgcagc acgctataat tatttatttg aattagagaa agaatatggg ttgaatttac
    55561 gtccattgtt ttcttttgct catcaaactt atcagaagaa tccagtattt gcgcctaaaa
    55621 acagtaaaaa tctatccgaa agagagcaag aagaattaga aaaaatccat caggcacttt
    55681 ctatccttca attcaaatca gaacatcagt tattggatcg tcgacctgaa tttaaaatgg
    55741 acgatcgtaa attgctggaa aaaatcgact atgaagcatc aacgattgat ttagaaggtc
    55801 agctctatga gttgaaagga acttgttttc aaacgatcca gccagatgat ccaaaaaaat
    55861 tcttggaaga ggaacaaaaa gtgattgata gtttgatgta ttcattccag cactctttga
    55921 gaatgcaaaa acatatgacg tttttgatcg aaaaaggggg aatgtatctc acgaacaatc
    55981 agcatgtgct gtttcatggc tgtatacctg tagacgagaa gggacagctc ttagcatttg
    56041 aattagacca atcttatcgc ggaaaagaac tgctgggctt ttttgaaaaa cagataactg
    56101 aaagcgcaaa agatctgact gcaaaagaag atttagcaac cgatttgatt tggtatgcgt
    56161 ggagtgggcc attttcgcct ttattcggca agagtaaaat ggcgactttt gaacgttatt
    56221 ttctcacaga gcctcatacg cacgtcgaga aaagcaatcc ttattatcac ttgcgagatg
    56281 aagagtggtt cagtgaaaaa attttagcag aattcgacac aaaagaagaa gggtcagcaa
    56341 tcattaatgg gcacacgcca gtaaaagtaa aaaaagggga gtcaccgatc aaagcaaatg
    56401 gaaaagtatt tgtcattgac ggaggtttat ccaaagctta tcaaaaaacg acaggaattg
    56461 ccggttattc actattatgc aattcttatg gtttccaaat cgtgacacat tatccgtttt
    56521 tgcctattga acaacaattt gcaaaaaaat ccgaccaaac aaatgtgaaa aaagtaatcg
    56581 aacagcaact accacgcaaa ctcaatcgtg atacaacaaa aggtatggaa ctgcagcagc
    56641 agattaccca gctcaaaagg ctgttggact atcgaaagga cgattgagaa gaattccagt
    56701 gaacattgtt ccatagactg gagactgaga caaaagtcgt gtcgcagtct ctttttccgg
    56761 ataaacggtg ttcttctgcc aactcctcgg tcacaagtca cactattcaa caaacatgaa
    56821 aagctgtttt ttgaaattgc tccttgtgac tcagagttga acggcaggct cacgacctct
    56881 tggtataaac ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg
    56941 tgaaaagcca ttttcacaaa atttttctta atactcgaaa ctaaacagct ttttcacaac
    57001 cccctcagaa agtctattaa aattcattca caaagaaatc gaataagcgc tgttcagatg
    57061 gatgtacacg gtgggtaagt tccggatgcc attgaacacc gaggatttta tgcatatcat
    57121 ctttgctttg aaccgcttca acgagtccat cgttagaaaa agcgatggct tttaataaag
    57181 gtgaaagatc tttgactgcc tgatgatggt aagaattcac aaaatatgga tcggggagaa
    57241 ggttgcttaa caaactgtct gaaacaatct tgacttcgtg agtcgtaaat tgcggggcag
    57301 tgggctgttg ttcatgtttt actgaccact ttggatataa tgaaagatct tgataaagtg
    57361 taccttccaa aacgacattc aatagttgga gtccccggca gactgcaaaa attggttttc
    57421 tttgtttcaa tgcttccaag atcagtgcct gttcaaattg gtctcgttga atatttgttt
    57481 ccgttagctt tggatgagga tcttccatgt aaagttgtgg actgacatct tgaccacctg
    57541 caagaataag tttgtcgatt tgtccaatca gttgaggagc tgaatctgga gacatgactg
    57601 gcagaataag aggcaatcct tgagcatcta aaactgcatc cacgaatcct tgcggtgtat
    57661 agctcacttg atttccttgg aaagtatctg ttgctcgaat cagttgattt ccggcaatgc
    57721 cgatgattgg tttcatttgt attcccccta aagtagactt attgtaacaa ggatttaaaa
    57781 aaaattaaac tatgtaagac ttgcccctat acttatctgt tcttgctgtg ttactatctg
    57841 taaagagaaa tttcgtgtga aattcatgac gatagttgaa aatattgctt aatttgttat
    57901 aataaaaagt aagcagaaat aaaaaggttg ggcgtaagct tcaaggatct agttcttcaa
    57961 ggggtgagaa ggacatttta ttgaaggggc ctgcctatac taagttatct ttagaggtac
    58021 atactttcaa tgaaaaaaga aagcatgtac ctctttttta aatcgaaagg aagtgaagca
    58081 agtgttagaa gctttggaac aaatccgaaa agcagaagaa gaaaatgaaa agcgtgctca
    58141 aacactgcgg cagcagctga agatctatgc aacagaaaaa gagcaggaat tggaaacgat
    58201 ccaaacaaac tatcgacaac aactaaaaca gttggtggaa gaaaaagaac aatttgaaat
    58261 acaagaatta gaaaaagaaa aaatgaaatt gaaagcagaa gcagacaagg tgcaagcaac
    58321 tttgaaagat caatatcaaa agcatcagag tcaagcgatc ggtgcgatca ttgaaagggt
    58381 gaaagaaact tatggccgtc actaagatgg aaaaagtgac cctcatttca gataaaagaa
    58441 atcaggagag gattttacaa gcagtacaag gaatccaaaa cgttgaaatc cgggatctgt
    58501 atcaaaatag aacaaacaac cagtgggtgg agaagtattt tccagatacc acgattattg
    58561 ataaagaagc aaaattaagc gctttaggta atcggttgaa agacatccaa gaagctatcc
    58621 aattcataca acaccacggc gatagccgtc aaaagaaact ccatttgaaa cgtcaagaac
    58681 tttccttaca agatttggaa gccaattatt ccgaagaaaa tttcataaat aaattgaaag
    58741 aagtccttga tttaaaagaa aagtgggaac aactaactga agaacgtgat cgtttagaag
    58801 aagaggaaga atggcttctt cattggcaac agctggatat tgctccaagt atgtatgcaa
    58861 gtaagacgac catttttgag atggggactg tgagtgcagc aaattttgaa agtttcaagg
    58921 aagaactgag taagcttgat gacgtttata tggaagaaat agattatacg ccaaaacaag
    58981 tttatctttc gtatgttgct ttaaaaaaat cagttgctgt tatagaagaa attgctggga
    59041 aatacgggtt caataaagaa acgtatcctt atgaacaaaa tcctaaagaa aaactgcaag
    59101 agaccaagca gcaattatcc attgttcacg agcagcaaaa gaagataacg acagcgttag
    59161 gtcagtgcag tggctatatc caagaatttg aatgggtaga agaagtcaca cttgccgttg
    59221 cagaacgaga aaaaataaaa gaacggttta tccatgcgac ttatctgatc gttttgcaag
    59281 gttgggtaga tgcagaagaa aaacagaatt tgctgcatgt gttaaacgaa accgtatcag
    59341 aagaagatat atacgtcagt ttcgaaacac caaactcaga agagattcag caggaagtcc
    59401 ctactaaatt gaaaaaccat ccgcttgtcg aaccttttga attgctgact gaaatgtaca
    59461 gtctgccaaa atatgaagaa gtagatccaa caccttggat gacaccattt tatcttgtct
    59521 tcttcgggat gatggtcgca gatgttggtt atggtttgct gatgctcatt gggacaatac
    59581 tagctcaaaa actacttgtt ctaccacgcg ggatgaaacg tttcgtaaaa ttctttgaga
    59641 ttctctcgat accatcgatc atctggggat tgatctacag ttcatttttc ggacagtcat
    59701 taccaaaaga actgtttggc atccgcttgc ctttcccaat cctatctacg acggatgacg
    59761 tcaatacgat tttgatttta tccgtgattt tcggtctgat acagattttg gttggattat
    59821 ttgtggcagc aaaggaaaat atcaggagaa aagattactt gagtgctgtc agtgatggat
    59881 ttgcctggca agggatccta attgggatcg ttattgcgtt ggttggtgcc atgttgataa
    59941 aaaatacagc atttgtttac cttggcgctg ggctatcgat cattgctgcc ttatgtatct
    60001 tgatcgttcc gattatccaa acgccgtcta aagtcaaagg tgctgccaaa ggtgcgtata
    60061 atctttacgg actgaccggc tatatcggtg atttagtcag ttatacacgt ttgatggcat
    60121 tgggtatttc aggcggaagt attggagcgg cattcaatat gctagttgct ttcatgccgc
    60181 cagctgctcg attcagtgta gggatccttt tgatcatcgc tttacacgca ctgaatatgt
    60241 ttttgacact gctaagtgcc tatgtccatg gcgcacgatt gcagtatgtc gaattttttg
    60301 ggaagtttta tacaggaggc ggacgtgcct ttgatccgct gaaaacggcg gaaaaatacg
    60361 tcaacatcaa tcacaagaaa aaaaacaata aataattgga ggatttttat ataatgatgg
    60421 attacttaat tagtcaaaat ggtggaatgg tatttgcggt attagcgatg gcaacagcga
    60481 cgattttttc agggatcggt tcagctaaag gtgttggaat gacaggtgag gcagcagcag
    60541 cattgacaac aagtcaaccc gaaaaattcg ggcaggctct gattctgcaa ttgcttccag
    60601 gcacgcaagg tttgtatgga ttcgttattg cctttttgat ttttatcaac ttaggaaatg
    60661 acatgtcagt tgtacaagga ctgaactttc taggtgcttc tctgccgatt gcttttacag
    60721 gcttgttttc aggtattgcc caaggaaaag tcgcagcggc aggtattcaa attttagcga
    60781 aaaaacctga acatgctaca aaaggaatca tttttgctgc gatggttgaa acatacgcga
    60841 ttttaggttt cgttatttcc ttcctattag tgttgaatgc ctaagcataa aggaggaaaa
    60901 gcagatgaac gccattgaaa aaatcatctc tcagatgaac gaagcagcag agcaggaaag
    60961 agcggcgtta gaacaagaag agcgaatgaa gatcgatcaa aactttgaac aaaaacgtac
    61021 gcaagttgaa acagaacatc aaaaacaaaa agaaaaacaa atcgaattac tcgaaaaaaa
    61081 ataccgtcag ctaaggaatc gccagcaagt agaagtacga caagaaaatt tgaatgcgaa
    61141 gcaggaattt ttaagacgat tgtttgcaga tgcggtaact gaaatggaaa attgggatga
    61201 atctgaacaa atccagttta tcaaaaatgc attgtattca ttacctttga caggaaaagt
    61261 agcattcatt gctggagaaa aatcagcagc ctatctttct cagacgttgt tggatgaatg
    61321 gaacaatgaa cttcccttta tgatggtttt aagtgatgaa actgttgctg atcaagcggg
    61381 ttttctgata aacgatcaag gagttcagta taattttctt ttcagcagcc ttgtacaaga
    61441 tatccaagga acgatgagtt ttgagattgc caatcaatta ttcgaataag gagggatcag
    61501 tgtatggaat accacgaact aaatcccttg attcgcggaa gagagcttga acttttgtcc
    61561 aatgagacat ttgaacggat gattcaaaca aattctgtcg aagcacttgg tgaaatattg
    61621 aaatccacga tttacagtcc atatatttat gacggatatg aatatgactt cgaacagaat
    61681 cttttaaagg agcagagccg tctatttcgc tggttaaagg aatcagcacc ggagcctgaa
    61741 atcgtttgga tctatacgat gcgttatact ttccataatc tgaaggtttt gacaaaagct
    61801 gagatgacaa atcaaaatct ggaccatttg tatatcgacg atggatttta ttcattagaa
    61861 accttgaagg atgccattca tacgcaagtt tcaaatgaac ttcctgaaag cattatggac
    61921 agtatacgag aagtacgtga ttattttgaa gagtctacca ttcttcaggg aattgacgtg
    61981 atttatgatc gacagttttt gacagagcag cgacggcttg ccgataagct aggatatcaa
    62041 gaattgctga cagaagtcat tgcttttatt gacttgacca atatcacaac gatggctcga
    62101 ggaatattgc agaaaagaac atatgggttc atgacgacgg tactttccag ctcgggaagt
    62161 atcgaaaaag aagtattttt gaactttgta gataaagata tggaagatta cacgaatttt
    62221 ctattagaga cagattatgc tgattttctg cgccctgtca tccgtgaggg aaatattgat
    62281 ctcgtaaagc ttgaacagtt gaaagatgat tatctaagtt cattgtatca agaagcacaa
    62341 acccaggctt tcggaccact gccactacta gcctttttga acgcaaaaga ggtagaaagc
    62401 aaaaaccttc gtttactggt tatcggcaaa agaagccatt tcgaatcaga aacaatcaga
    62461 aagcgggtga gacaagtcta tgacgcataa gatcggtgta gttggcgaca aagattctgt
    62521 tttgccattc aaattgtttg gctttgatgt tcgttatggt acaaccaagc aaaccgttcg
    62581 tcaggcaatt gaagaaatgg caaagaatga gtatggcgtg atttatgtca cagagcaatg
    62641 tgcagcattg agcccagaaa cgatcgaacg ttacaaggaa caaatgatac ctgctatcgt
    62701 attgattccc aatcatcaag gatcattagg gatagggctg gaagaaattc aaaaaaacgt
    62761 agaaaaagca gtaggtcaaa atatcttata gtatgaaaag gagatagaaa ttttgcaaat
    62821 tggaaaaatc ataaaagtct ccggacctct tgtaatggct gagaacatgt ctgatgccag
    62881 tatccaagac atgtgtctag tgggagatct aggagttatc ggagaaatta tcgagatgcg
    62941 tggcgatgtg gcgtctattc aagtatatga agagacttct ggaattggtc caggcgaacc
    63001 ggttcgctct actggtgaag ctttatctgt tgaattagga ccagggatca tttcacaaat
    63061 gtttgacgga atccaacggc cattagatac ctttatggaa atcacacaaa gtaatttctt
    63121 agggcgcgga gttcaattgc ccgcattgga ccatgagaaa aaatggtggt tcgaaccaac
    63181 agtagaagcc ggagaaacgg tgagtgctgg agatgttatc gggatcgtag aagaaacaaa
    63241 agttataaaa cataaaatca tggtgccaaa tgggataaaa ggaacgatca aaacaatcga
    63301 aaaaggttca ttcactattg atgaaacagt atgtgtcatt gaaaccgaaa atggtgacaa
    63361 aaatttgacg atgcttcaaa aatggccagt aagacgtagt cggccaatca aagaaaaatt
    63421 aaacccggat gcacccatga tcactggaca gcgagtgatt gatacattct tcccagtgac
    63481 aaaaggagga gcagcagctg tacctggtcc ttttggtgca gggaaaacag tcgttcagca
    63541 tcaaattgca aaatgggccg atgtggacat ggttgtctat gtgggatgtg gcgagcgtgg
    63601 aaatgagatg acggatgttt taaatgaatt ccctgaattg attgacccta atacaggaga
    63661 atcgttgatg gaacgtacgg tattgattgc taatacatca aacatgccag tagcagctcg
    63721 agaagcttct atctatacgg gaatcacaat tgcggaatat ttccgtgaca tgggttatga
    63781 cgttgctatc atggcagact ccacttcacg atgggcagaa gcacttcgtg agatgagcgg
    63841 acgtttggaa gaaatgcctg gtgatgaagg ttatccagct tatcttggtt cacggcttgc
    63901 ggaatactat gaacgatctg gaagagtcat tgcattagga tcagaaggtc gcgaaggaag
    63961 tataaccgca atcagtgctg tatccccgtc tggaggggat atttcggaac cagtcacaca
    64021 aaatacattg cgggtcgtaa aagtattctg gggtcttgat tccagtttag cccaaaaaag
    64081 acattttcct tcgatcgatt ggatacaaag ctattctctt tattcaacag aagtagggaa
    64141 gtatatggat catatccttc aagctgactg ggcatccatg gtcaccgagg gcatgcgtat
    64201 tttgcaggaa gaacagcagc tgaatgaaat cgtgcgtttg gtagggattg attccctttc
    64261 agataatgac cgtctgacat tagaagtggc aaaatccatt cgtgaagatt atttgcagca
    64321 aaatgctttt gatgatgtcg atacattcac ttcaagagag aaacaattca aaatgttgaa
    64381 gatgatcttg acctttggtg aagaaggacg ccgtgcgttg tcgttaggtg cttatttaag
    64441 tgagatcatg gacggtaccg tatcgattcg tgaaaggatc ggacgaagca aatatatccc
    64501 ggaaacagaa ttagaaaaac tagatcaaat tattggtgaa atcaagcaaa cgatgcaaac
    64561 gatcgtttca gaaggaggca tgagcaatga ttaaagaata tcgtacgatc ggtgaagtcg
    64621 tcggtccatt gatggcggtt gaaaaagttg ctggtgtcaa atatgaagaa ttaatcgaag
    64681 tccgtatgca aaatggcgag attcgtcgtg gacaagtatt ggaagtccaa gaagataaag
    64741 caatggtcca aatattcgaa ggaacaagtg gtatcaattt acgtgactct tctgtacgat
    64801 ttttaggcca tcctttagaa ctaggtgtat cggaagacat gatcggtcga gtattcgatg
    64861 gtttgggacg accgaaagat aacggaccag agatcctgcc tgaaaagcat ttggatatta
    64921 acggagaagt aatcaatcca gtagctcggg attatccaga tgagtttatc caaacaggta
    64981 tctctgcaat cgatcatttg aatacattag ttcgtgggca aaaattacca gtattttctg
    65041 gctctggtct tcctcataaa gaattagctg ctcagatcgc gcgacaagca acggtactcg
    65101 attcatcaga tgatttcgcc gtagtctttg ctgctattgg aatcactttt gaagaagctg
    65161 aatttttcat ggaagatttt cgtcaaacgg gcgcaatcga ccgttctgtc atgtttatga
    65221 atttagcaaa cgaccctgca atcgaacgta tcgctactcc tcgtatggct ttgactgctg
    65281 cagaatatct tgcttatgaa aagggaatgc atgttttggt tatcatgaca gacatgacca
    65341 actatgcaga ggcattgcga gagatttctg cagcacggcg agaagttcca ggaagaagag
    65401 gatatcctgg gtatctttac acgaacttag ctactttatt cgaacgagca ggaagaatcc
    65461 gcggactaaa aggatcggtt actcaaattc cgattctaac aatgccagaa gacgataaaa
    65521 ctcatccaat ccctgacttg actggttata ttacagaagg acagatcatc ttgactagag
    65581 agctttacaa gagtgggatt cagccaccaa ttgatgtact cccgtctctt tcgcggttaa
    65641 aagataaggg aactggaccc ggaaagacaa gagaagacca tgcagctacg atgaatcagc
    65701 tttttgcagc ctatgcacaa ggaaaacagg caaaagaatt agccgtagtt ttaggtgaat
    65761 ctgctttatc tgatgtagac aagatctatg cgaagtttgc tgagcggttt gaaaaagaat
    65821 atgtcaatca aggattctac acgaaccgtt cgattactga aacacttgat ctagggtggg
    65881 aactgctatc gatgttgcca agaaccgaat taaaacgaat caaagacgac atgctggaca
    65941 aatatctgcc agaagggaag tgagttctga tggcacgatt gaatgtgaat ccgacccgca
    66001 tggaattgac tcgattgaaa aaacagttga caactgccac aagagggcat aaattattaa
    66061 aagataagca agatgaactg atgcgtcaat tcatcctgtt gattcgaaag aacaaccagc
    66121 tgcgtcaaga aatggaagag tctatgacag aagcaatgtc tgattttgta ttggcaaatg
    66181 cttcgatcac ggaacctttc atcgaagaat tatttgtttt gccggcggaa aacgttgatt
    66241 tgtctatcgt agacaaaaat atcatgagtg taaaagtacc tgtgatgaat tttgaatatg
    66301 atgaacagct gacagacaca cctttggaat acgggtattt gaattccagt gccgaactgg
    66361 atcggtcaat cgatcggttt actaccctgc ttccaaaact tttggaactg acagaaatcg
    66421 aaaaaacttg tcaactgatg gcgagtgaaa tcgaaaagac acgtcggcga gtcaatgcat
    66481 tagaatatat gacgatccct caattagaag aaacgatcta ttatattcgt atgaagctgg
    66541 aagaaaacga gcgcgcagaa gtaacaaggt tgatcaaagt aaaaaatatg ggtaccactg
    66601 actaagtggt atcttttttt aaaagaggaa aacgatgatt aaacaaagca aacaaataaa
    66661 gaaacgtttg ccggctgttc agcttctcgc attaggattt tttctcctga ttttagttgg
    66721 cggcggccta cttaccctac cattttttag taatagtggg catgggacaa attttatcga
    66781 tgcgcttttc actgcaactt ctgctgtctg tgtaacggga ttgacaacat tgaatacagc
    66841 agagcattgg aacagtgctg gtcaattttt gatcatgctt ttgattgaga tcggcggtct
    66901 tggttttatg atggttccga ttctatgctt tgcattgata aagaagaaag tcagtctcag
    66961 cacacgtatt atattaaaag aagcgctgaa tctagaagaa gtatcaggtg tgatgaagct
    67021 catgctttac atcctaaaat tggcctttgt gatccaattg attggtacgg ttgctttatc
    67081 ttttgtattt gtgcctgaat ttggctggtt gaaaggtatc tggtacagtt tgttccatgc
    67141 aatctctagt ttttgtaatg cagggtttga tttgttagga gacagtttag ccaatcacca
    67201 gcaaaatgtt tatttgatca tggtggtatc tgctttgatc atttctggtg ggttaggctt
    67261 catcgtatgg cgtgatttac tggaatatca tcagaaaaag aagatgacga ttcattcaaa
    67321 aatcgcatta accgttacag gtaccctttt attaggcgga ttcgttgtgt tctttttcac
    67381 agagcgcaat ggggcaacgc tagtggaagg gaatatgttt caacgattgg caaacacctt
    67441 ttttatgagt gtgacaccta gaacggctgg atattattca gttgattacc tccatatgag
    67501 ccatgcaggt ctgattttaa cgatgttttt gatgtacatc ggcggaacat ctgggtcgac
    67561 ggccggcgga ttgaaaacaa ctacgttagg tgtgttgctg atccagatgc gttcgatgtt
    67621 gaaaggacgt acgagagcgg aagcatttgg ccgtaccatc cgacaaggcg cagtgttgcg
    67681 ggcacttact ttattttttg ttacgttgac gttgtgtgta gtcgccatca tgattttatc
    67741 ggtgactgaa acgataccta aaacttctgg catcgaatat attgcttttg aggtcttctc
    67801 tgcgtttggt acagtaggtc ttacgatggg attgacgcca gacttgactg caatagggaa
    67861 attgatcatt atagcgctta tgtacattgg acgagttggt gtattgactg tggtcttctc
    67921 tctgatgaca aaagcacaaa gaacacaatc aaaatacaaa tatccagaag aaagtgtcat
    67981 gattggataa tttcatcttc tgacagaaac ctagtgattt agtaaaataa gaaaataaaa
    68041 taatttttct tgcttcttgg atactttaag tgcaaaatgt tcgtttattt tttgaggcac
    68101 tacccgttgt tgaacctcgg gtagtg
//
LOCUS       DAMYXM010000007        64389 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.007, whole genome shotgun sequence.
ACCESSION   DAMYXM010000007 DAMYXM010000000
VERSION     DAMYXM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.007"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..556)
                     /locus_tag="PNS21_000551"
     CDS             complement(<1..556)
                     /locus_tag="PNS21_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDI5158285.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKH"
     gene            complement(633..1163)
                     /locus_tag="PNS21_000552"
     CDS             complement(633..1163)
                     /locus_tag="PNS21_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDI5158286.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1167..2027)
                     /gene="rlmB"
                     /locus_tag="PNS21_000553"
     CDS             complement(1167..2027)
                     /gene="rlmB"
                     /locus_tag="PNS21_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDI5158287.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2020..2415)
                     /locus_tag="PNS21_000554"
     CDS             complement(2020..2415)
                     /locus_tag="PNS21_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDI5158288.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2412..3824)
                     /locus_tag="PNS21_000555"
     CDS             complement(2412..3824)
                     /locus_tag="PNS21_000555"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDI5158289.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3821..4357)
                     /gene="cysE"
                     /locus_tag="PNS21_000556"
     CDS             complement(3821..4357)
                     /gene="cysE"
                     /locus_tag="PNS21_000556"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDI5158290.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4443..4682)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4679..6139)
                     /locus_tag="PNS21_000557"
     CDS             complement(4679..6139)
                     /locus_tag="PNS21_000557"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDI5158291.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6309..7466)
                     /locus_tag="PNS21_000558"
     CDS             complement(6309..7466)
                     /locus_tag="PNS21_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDI5158292.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7500..8870)
                     /gene="radA"
                     /locus_tag="PNS21_000559"
     CDS             complement(7500..8870)
                     /gene="radA"
                     /locus_tag="PNS21_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDI5158293.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8933..9460)
                     /locus_tag="PNS21_000560"
     CDS             complement(8933..9460)
                     /locus_tag="PNS21_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDI5158294.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9690..10496
                     /gene="proB"
                     /locus_tag="PNS21_000561"
     CDS             9690..10496
                     /gene="proB"
                     /locus_tag="PNS21_000561"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDI5158295.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10493..11740
                     /locus_tag="PNS21_000562"
     CDS             10493..11740
                     /locus_tag="PNS21_000562"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDI5158296.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11816..12160
                     /locus_tag="PNS21_000563"
     CDS             11816..12160
                     /locus_tag="PNS21_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158297.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12277..12603)
                     /locus_tag="PNS21_000564"
     CDS             complement(12277..12603)
                     /locus_tag="PNS21_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158298.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13219..14421)
                     /locus_tag="PNS21_000565"
     CDS             complement(13219..14421)
                     /locus_tag="PNS21_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5158299.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14538..15053
                     /locus_tag="PNS21_000566"
     CDS             14538..15053
                     /locus_tag="PNS21_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDI5158300.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15252..15698)
                     /locus_tag="PNS21_000567"
     CDS             complement(15252..15698)
                     /locus_tag="PNS21_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDI5158301.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15745..17223)
                     /gene="msr(C)"
                     /locus_tag="PNS21_000568"
     CDS             complement(15745..17223)
                     /gene="msr(C)"
                     /locus_tag="PNS21_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDI5158302.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17577..18206)
                     /locus_tag="PNS21_000569"
     CDS             complement(17577..18206)
                     /locus_tag="PNS21_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDI5158303.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18190..18654)
                     /locus_tag="PNS21_000570"
     CDS             complement(18190..18654)
                     /locus_tag="PNS21_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDI5158304.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19141..20409)
                     /locus_tag="PNS21_000571"
     CDS             complement(19141..20409)
                     /locus_tag="PNS21_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDI5158305.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20640..21377)
                     /locus_tag="PNS21_000572"
     CDS             complement(20640..21377)
                     /locus_tag="PNS21_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI5158306.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21444..22013)
                     /locus_tag="PNS21_000573"
     CDS             complement(21444..22013)
                     /locus_tag="PNS21_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDI5158307.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22141..23013
                     /locus_tag="PNS21_000574"
     CDS             22141..23013
                     /locus_tag="PNS21_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI5158308.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23355..24227
                     /locus_tag="PNS21_000575"
     CDS             23355..24227
                     /locus_tag="PNS21_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDI5158309.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24224..26182
                     /locus_tag="PNS21_000576"
     CDS             24224..26182
                     /locus_tag="PNS21_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDI5158310.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26514..27365)
                     /locus_tag="PNS21_000577"
     CDS             complement(26514..27365)
                     /locus_tag="PNS21_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI5158311.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27488..27955
                     /locus_tag="PNS21_000578"
     CDS             27488..27955
                     /locus_tag="PNS21_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDI5158312.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(28218..28700)
                     /locus_tag="PNS21_000579"
     CDS             complement(28218..28700)
                     /locus_tag="PNS21_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDI5158313.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(28800..29663)
                     /locus_tag="PNS21_000580"
     CDS             complement(28800..29663)
                     /locus_tag="PNS21_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDI5158314.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            30261..31136
                     /locus_tag="PNS21_000581"
     CDS             30261..31136
                     /locus_tag="PNS21_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158315.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(31281..31433)
                     /locus_tag="PNS21_000582"
                     /pseudo
     CDS             complement(31281..31433)
                     /locus_tag="PNS21_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(31701..33032)
                     /locus_tag="PNS21_000583"
     CDS             complement(31701..33032)
                     /locus_tag="PNS21_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDI5158316.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(33181..33894)
                     /locus_tag="PNS21_000584"
     CDS             complement(33181..33894)
                     /locus_tag="PNS21_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI5158317.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            34069..35493
                     /locus_tag="PNS21_000585"
     CDS             34069..35493
                     /locus_tag="PNS21_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDI5158318.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            35792..36499
                     /locus_tag="PNS21_000586"
     CDS             35792..36499
                     /locus_tag="PNS21_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDI5158319.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            36486..36809
                     /locus_tag="PNS21_000587"
     CDS             36486..36809
                     /locus_tag="PNS21_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDI5158320.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(36898..37935)
                     /locus_tag="PNS21_000588"
     CDS             complement(36898..37935)
                     /locus_tag="PNS21_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDI5158321.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(38256..39239)
                     /locus_tag="PNS21_000589"
     CDS             complement(38256..39239)
                     /locus_tag="PNS21_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDI5158322.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(39654..40424)
                     /locus_tag="PNS21_000590"
     CDS             complement(39654..40424)
                     /locus_tag="PNS21_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5158323.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(40417..41352)
                     /locus_tag="PNS21_000591"
     CDS             complement(40417..41352)
                     /locus_tag="PNS21_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5158324.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(41668..43317)
                     /locus_tag="PNS21_000592"
     CDS             complement(41668..43317)
                     /locus_tag="PNS21_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDI5158325.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(43434..46037)
                     /locus_tag="PNS21_000593"
     CDS             complement(43434..46037)
                     /locus_tag="PNS21_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDI5158326.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(46049..47098)
                     /locus_tag="PNS21_000594"
     CDS             complement(46049..47098)
                     /locus_tag="PNS21_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDI5158327.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(47124..47438)
                     /locus_tag="PNS21_000595"
     CDS             complement(47124..47438)
                     /locus_tag="PNS21_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDI5158328.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(47454..47900)
                     /locus_tag="PNS21_000596"
     CDS             complement(47454..47900)
                     /locus_tag="PNS21_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI5158329.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(47901..49823)
                     /locus_tag="PNS21_000597"
     CDS             complement(47901..49823)
                     /locus_tag="PNS21_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDI5158330.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(50035..50931)
                     /locus_tag="PNS21_000598"
     CDS             complement(50035..50931)
                     /locus_tag="PNS21_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDI5158331.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(50924..51604)
                     /locus_tag="PNS21_000599"
     CDS             complement(50924..51604)
                     /locus_tag="PNS21_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI5158332.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(51605..52387)
                     /locus_tag="PNS21_000600"
     CDS             complement(51605..52387)
                     /locus_tag="PNS21_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5158333.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(52380..53297)
                     /locus_tag="PNS21_000601"
     CDS             complement(52380..53297)
                     /locus_tag="PNS21_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI5158334.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(53461..53784)
                     /locus_tag="PNS21_000602"
     CDS             complement(53461..53784)
                     /locus_tag="PNS21_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158335.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            54572..57799
                     /gene="fss3"
                     /locus_tag="PNS21_000603"
     CDS             54572..57799
                     /gene="fss3"
                     /locus_tag="PNS21_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDI5158336.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            57912..58226
                     /locus_tag="PNS21_000604"
     CDS             57912..58226
                     /locus_tag="PNS21_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDI5158337.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            58239..58613
                     /locus_tag="PNS21_000605"
     CDS             58239..58613
                     /locus_tag="PNS21_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDI5158338.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            58623..58958
                     /locus_tag="PNS21_000606"
     CDS             58623..58958
                     /locus_tag="PNS21_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HDI5158339.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            59041..60381
                     /locus_tag="PNS21_000607"
     CDS             59041..60381
                     /locus_tag="PNS21_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDI5158340.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            60459..61133
                     /locus_tag="PNS21_000608"
     CDS             60459..61133
                     /locus_tag="PNS21_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDI5158341.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            61126..61290
                     /locus_tag="PNS21_000609"
     CDS             61126..61290
                     /locus_tag="PNS21_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158342.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            61366..62508
                     /gene="dcm"
                     /locus_tag="PNS21_000610"
                     /pseudo
     CDS             61366..62508
                     /gene="dcm"
                     /locus_tag="PNS21_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            62498..62788
                     /locus_tag="PNS21_000611"
     CDS             62498..62788
                     /locus_tag="PNS21_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158343.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            63047..64231
                     /locus_tag="PNS21_000612"
     CDS             63047..64231
                     /locus_tag="PNS21_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDI5158344.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            64228..64365
                     /locus_tag="PNS21_000613"
     CDS             64228..64365
                     /locus_tag="PNS21_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDI5158345.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT        19269 a        12975 c        11705 g        20440 t
ORIGIN      
        1 agtgtttttt tattttgagt tttactctcc cgtaagcgct cattacttgc ttgtgatcca
       61 ttgctaaaca ttcagccatg tctacaaaat caacgccttg catataataa tgaaaaattt
      121 tagcttctat ttttgaaaac tggatttcgc aattttcgat tgcttctttt aataaaagat
      181 gctgaattgc taaaggttct tgcaatgtga tctctgctaa aaagtctggt ccttctgtct
      241 ctattttttg gtcgattgag acttcatctt tttgatcacg acgcttcagc gcgttctgct
      301 tcctcaacag agaatagatt tgattgctta taacgctttt ataataactt ccaaagctaa
      361 cacctttgct tttttcgtaa gttctcaatg cctgatataa agcgatcctt gcttcttgtt
      421 cccaatcctc agaaacataa tttcttaaat aataggtgtt tttcaaacgt agtaaaagtg
      481 gaaaatattt ttgatacagg gcagcaaatg cttcttcgtc accattacgt gcagcgcgca
      541 ataactcctc agacataaaa taattcctcc ataaattcgt ttatggagga atcttatcat
      601 caatgattca tcgaactgat tagaacgata tgttatttat tttttctcat taaatcgtcc
      661 aatttttggg acaatttttt taactgctct tcattccatg gagaattacg tcggaaatca
      721 ataaaatgca tatctttatt ttcttgggct attgtttttt ccgttttttt tatcgtctta
      781 tataattcat aagcagaagt tcgtaatgct ccttgggaaa agatcatcca ctgttctgct
      841 aaatcgcttg tcgccactgt cacttgtgtc aaacggtcat tcagctgacc agcgattctc
      901 tcgatataac tgtcagccgt ttcgtcttct ttcgtaaaaa caacatccaa ctggtatttc
      961 ttataatttt gttggatgcc cggaactagt tgggcatcga agacaacgat gacttccatt
     1021 ccttcatatt tcgcataatt tgataaacga tgtaaaagag cttctcttgc atccgccatt
     1081 ttgtcctgct ttttaagctg gacaagttcc ggccacgaac caatcatgtt gtagccgtct
     1141 acaatcaaca gctgtttttt catggtctac tccccctgac gttgggaaaa agcctgatac
     1201 ataagcaaac cagcagcaac actggcattt aagctctgga cgtgcccggt catcgggatc
     1261 gtcagcaatt catccatttc ttttttcaat cctggactca ttcctttgcc ttcattacca
     1321 atgactaaag caattgatcc tttggcattc catttttgga agtttgtgcc ggtcatatct
     1381 gtgccaaata cccaaaatcc tgtttctttg agctgttgca cagcttgtac caaattcgtc
     1441 acgcgtgcaa cagggacatg ttcgactgcc ccagtggaag tctttgttac gataggtgtg
     1501 attccaactg ctcggtgttt tggtatgatc acaccatcga cgccacttgc gtctgctgtc
     1561 cgtaaaatcg acccaaaatt gtgtggatct tcgatgctat ccaaaatcaa aaagaagggg
     1621 tcttcttttt tctcttttgt tcgttcaatc agttggtcaa gggataaata ttcataaggt
     1681 gtaatcgcaa gaacgactcc ttgatgcacg ccgtgatcgc tcaaagtttc taatttttgt
     1741 ttaggaaccc atttgactgg cacagcttgt tcacgtgcga tttctttcag ttcttctact
     1801 ttttctcctc gtgaatcttc ttgaagaaag agtttattcc ctcgcccttg ttgtagcgct
     1861 tctactgcgg catggtgccc aaagacgaaa ttatcttcta atgcttcttc attttgatca
     1921 gaaactgact gatttcgtcc agtttgcttt ttatttgaat atctattttg ttttttgtct
     1981 tcaaatccac gtttcgattg atgtttgttt gttctttctt tacccatttt tttcaccaac
     2041 tttctgaata caccagcgga tcaattcttc catccgctcg gtttgttttg tcaaatgcag
     2101 atagcccatg agcgcttcga accctgttgc gacccgatac gtcgtaacat cagcattttt
     2161 tgcgctcgta tggcttttag catttcttcc tctgcgataa taaagttctt cttcttctgt
     2221 caaaagatgt tcttccagca tttgctgaat caaaaaggct tgtgcattcg cagaaacata
     2281 attcgttgcc gcacgatgca gcttattggg ttttgtttct ccttgatcaa tcaggtggtc
     2341 acgaacatag atttcgtaaa tcgcatctcc aacataagcc aaggctaaac cgttcaattg
     2401 tgtgtaatct ctcatgcttc tcttctccat cgggttcctt gcggtgtatc ttctaataag
     2461 atcccttttt ctttcaacaa atcacgaatt tcgtcgcttc ggacaaaatc acgattgctt
     2521 cttgcttgat ttcgttcttc gatcaattgt tcaatctctt catctaataa ctcttctgct
     2581 gtaaataaaa taccgaagat cgaaagccat tgcgagaata aagcttctcc ttgtgccaag
     2641 acagcggcag aaactttcgg ctggtctaga taagtgttca gccacttagc aagctcatag
     2701 accactgtga tcccatttgc tgcattaaaa tcatcgtcca tttcttcgat gaatcgctgt
     2761 tctaaatttt ctaacaaagc gattttatcc gcatcatctg ccaaattctc tgaggcagtt
     2821 tcttggcgga atttcaaatt gtcaaacgta ttcttcagtc gttgtaaatt ggtggctgct
     2881 tctttcatcg tcgtttcact gtagcggatc ggacgacggt attgtgtggt cgccatgaag
     2941 aaacgcaaga cttgtggatc aacgtttttg atcatttcat gtacagtgat gaaattccct
     3001 agagatttac tcattttttc atcgtcttcg ccaatcgtta catagccatt gtgcatccag
     3061 taattagcga attttttccc tgttttcgct tcactttgtg cgatttcatt ttcatgatga
     3121 ggaaattcta aatcttgccc gccaccatgg atatcgatcg tttctcctaa atgttttgtt
     3181 gccataacgg aacattcaat atgccaaccg ggacgtcctt tgccccatgg tgaatcccaa
     3241 gaaatctcgc caggttttgc ttgtttccat aaagcgaaat ctaatgggtc ttctttttgc
     3301 tgctgttctt cccctgtacg ctgacttgct ccgacttcca gttcatcgat ggattggtca
     3361 cttaatttcc cataattttc aaatttacgg gtacggtaat acacatcacc tgaagattca
     3421 taagcatagc ctttttcaac caacacttga ataaatgcta aaatatctgg catatgatcc
     3481 atgactcttg gatgcagtgt agcaggttcg acattcaacg ctttcgtatc ttcttcaaat
     3541 gctcggatga aacgatcagc aacttctggt gctgtaatac ctaattcgtt tgctgtacgg
     3601 atgattttat cgtctacatc cgtgaaattc gatacgaaat cgactttata gcctcgatat
     3661 tcaaaatagc gtcgaattgt atcgaaagca atggtgctac gagcatttcc gatatggata
     3721 taattataca cagtcggtcc gcagacatac ataccgacat gtcccttttg tatagggatg
     3781 aattcttctt tttttctggt caatgtattg tagattttta tcattctgat ctcctcactt
     3841 tttctccttt gattcgaacg acttttgctg gtactcctac cgctgtagcg tcgtctggaa
     3901 catctttcaa cacaacggca cctgcaccga ttttggcgtt ttttccgatc gttaccggtc
     3961 ctaatatctg cgcattcgca gagagcatgg ctccttgttt aacagttggg tgtcttttac
     4021 ccgtttcttt tcctgtgccg cctaatgtta cgccatggaa taaaatcaca tcgtcctcga
     4081 tttctgcggt ttcgccaatg acgattccca ttccatggtc gatgaacacg ccagttccaa
     4141 tcgttgctcc tggatggatc tcaacaccag ttaggaaacg ccagaattga gcattgatct
     4201 tcgccaataa aaagagccgg tgctgatata aaaaatgaga gaaacgatgc caaaacaagg
     4261 catgaaaacc agggtatgtc aaaacgatct cagctgtcga acgtgcagcc ggatcgtttc
     4321 tctttgctgc atcaatcgct cgggtaaacc aactcatgac tttccactcc tttcgtaaga
     4381 attttatgta ggaaatcatc ttcttataaa gaaaagaagc gccttttaaa taagtttcct
     4441 tatcaaaaga cgctttccgc gtggttccac ttttcttcat aatcaatgat tatctctgac
     4501 agctaacgcc tgttatacgt tcttggctac tttgtttcct ataaaacagt ttttcctgtt
     4561 ttctgtgttt cgccaagacc actcccagag gcatttcggc aaccattcat tgataccttg
     4621 caccacccgg tatctctctt gtaatgatca gctgcttact tttttctgtt catcgtgttt
     4681 agttcagtac ttgatttaaa tgtgctaatg atttttcacg accaagcaat tcgatcgttt
     4741 caggcaattc tggaccatgc atttgtcctg atacagctac acggattggc atgaaaagat
     4801 tttttccttt tacgccagtt tctttttgaa cagctttgat ggcagctttg acagttggag
     4861 catctactac ttccatttgt tctagctgtg ctttgaatgc gtggagaaca gtaggaactg
     4921 tttcaccagc aagtacttct tttgctgctt cattcaatac aggatgttca ttgaaaaata
     4981 gcgtagacag atcaacgatt tctgcagcat agctcatttg aggttgatac aggctaacga
     5041 tcttcttcac ccattcgatt gtcttttcat ctgggttttc tgctacacgt ccatcagcga
     5101 ttagataagg cagacacatg tcagtaaatg catctagatc cattgctttg atgtaatggt
     5161 tgtttaccca ttgcagtttt ttcgcatcga aagctgcggg tgatttactt aggcgttttg
     5221 gatcgaacat tttgatcaat tcttggtgtt cgaagatttc gtcttcgccc acaggtgacc
     5281 agcccaataa tgcaataaag ttgaacattg catctggtag atagcctaat tcgcgatatt
     5341 gttcgataaa ttgcaagatt gattcgtcac gtttgcttag ttttttacct gtttcactgt
     5401 tgatgatcaa tgtcatatgt ccgaaggttg gtggtgtcca gccaaatgct tcatagatca
     5461 tcagttgttt tggtgtattg gcaatatggt catctccacg taaaacgtga gtgattttca
     5521 tcaaatgatc atcgaccgca acagcaaaat tataagttgg cataccatcg cgtttttgga
     5581 tgacaaagtc gccaccgata ttatcggatt cgaacgaaat agcgcctttc accatatctg
     5641 taaatgcgta ttctgtattg cgaggcacac ggaaacgtac aacagattct agaccttgtg
     5701 cttctttttc tgcttgttct tcaggtgtca agttcgcaca tgttcctgcg taatgaggca
     5761 tttctcctct tgcgcgttgt gcttcccgtt ctgcttctaa ttcttcttct gtgcaatagc
     5821 atttgtatgc tcgattgcta gcaagcagct gatcaatcag cggttgatag atttctttac
     5881 gttcagattg acggtaaggg ccgtattctc caggattttc aggagattca tcccaattca
     5941 ttcccaacca agcaaggttt tccaactggc ttttttcgcc atcttcgata ttacgttttt
     6001 ggtcagtatc ctcaatacga ataataaagt ctccatcatt gtggcgggca aacaaataat
     6061 tgaataatgc agttcttgca tttccaatat gcaggtgccc agttggactt ggtgcatagc
     6121 gtacgcgtac tttcgtcatt ttcttcctct tctttcaaac tagactttga ctaacagcaa
     6181 aaattccgtc agttacggct tctcaaatat catttattta aagccactca gcacaaattt
     6241 taccttttct aaggtaaata gtaaagccaa ctggttgaat ttccttacca gttggctgaa
     6301 tttctttttt attttgattg tttagtcttt ttctctgtct cttttttatt gtcgattcct
     6361 cgtcctgcat gtgcaggttt agcaaagatc atgcgtccag cagctgtttg aagggcactt
     6421 gttacaacga cttcgatacg ttcattcatg taatgctgtc catcttctac gacaaccatc
     6481 gtgccgtcat caaggtaggc tactccttgt tggcgttctg tcccggcttt tactactaga
     6541 acattcatcg tttcaccagg gatgacgact ggttttacgg catttgccaa cgcattgata
     6601 ttaagaacag gtacattttg gaattcagag actttattca agttatagtc attggttaca
     6661 atgacaccat ctaaaagctt agccaatttg atcaatttgc tgtctacttc actgatatct
     6721 tcaaagtctc catcgtacat ttcgacagat ataccctctt ctttttgcaa ggcatttagg
     6781 atatctaatc cgcgacgacc acgcacacgt ttcaaactat cccctgagtc cgcgatgtat
     6841 tgtagttcgt aaagaacaaa attagggatc atcaaggttc cttctaagaa tccagtcttt
     6901 gtaatatcat aaatccgacc gtcaatgatc acgcttgtat ccaagatttt atatttatgg
     6961 aaattttctc ctgattttcg ttctaacagt tgactctcaa cttgactagc tgtttcttgc
     7021 tcgatttttt tgcttcgtga accaaaaact ttcttccatt cttcgattcg tgtcgttccc
     7081 attctcaatc ccagatagcc taagatgatc atcagtaaaa tcggcaatac actattgacg
     7141 aatggaattt ccatattgta aagaggggtc gagatcacaa cacctaaagt caatccgatg
     7201 atcgcaccga tcgcaccgaa gagaagataa gtcaagctga cctcacttag tttttgttct
     7261 acttgtttga taatagctga gatatgtttg gccaacagca aagataataa aaagaaaata
     7321 agtgcgccta acaggctgtt ggtaaatgta ttattcagcc atccgtttga ctgttgaccg
     7381 attgcctgcc aagctgaggg taaaaatgaa atacccaagc ttgcgccgac aatgatcatc
     7441 agcaaagtaa tgacacgttt ttgcatcgtt tttcctccgt ttcgacaatc atttattagt
     7501 tagcggaaca cacgcttcaa cgtttcagcc aaagttgcta ctccaactat ctcgattcct
     7561 tttggcgctt cccagttacc taagttgttt ttaggtaggt atacttttgt gaagccgagt
     7621 ttttggactt ctttgactcg ttgttcaata tgactgacac gtctgatttc tccagtcagt
     7681 ccaatttcac cgatgaaaca ttccgttggc tgtgttcctt tttctttata gcttgaagca
     7741 atactgacag cgatagctaa atcaatcgct ggttcattta attttacccc acctgcagct
     7801 tttaagtaag catcttgatt ctgaagaagc aacccagctc ttttttccag cactgccatg
     7861 atcagagata cccgattgaa atcaagacct gttgttgttc gtttggcatt tccgaacata
     7921 gtaggtgtga ctaatgcttg gatctctacc aaaattggac gtgtcccttc cattgccaca
     7981 acgatagcag agccagttgc accgtctagg cgttcttcta agaaaatctg tgaaggatta
     8041 gcgacttctt ccaatccatg ctcatgcatt tcaaaaatcc caatctcatt cgttgagcca
     8101 aatcggtttt ttactgctcg caaaatccga aaactatgat gttgttcacc ttcaaaatat
     8161 aacaccgtat ccaccatatg ttctaacatt cttgggccgg cgatcgagcc ttctttggta
     8221 acatgaccga cgataaaaat ggctatccca tttgttttag cggttttgag tagttctgca
     8281 gtggtttctc ttacttggct gacacttcct gccacacttg tgatgtctgg ctgcgtcatc
     8341 gtttggatgg aatcaatgat aacataatca ggggagatat gttcgatggc acgactgatt
     8401 tcgttcatat ctgtttcagc gtatagataa aattctgtgt tgatacttcc gaggcgttcc
     8461 gcgcgtaatt ttatctgctc tgcgctttct tcgccggata catataagac ttttccgcct
     8521 atggctgcta attgctgaga aacttgtaac agcaaagtgg atttcccgat acctggatcg
     8581 ccaccaatca agaccataga ccctggcacc acacctccgc ctaacacacg gttcagttct
     8641 tctaattttg ttttgatccg aggttctttt ttagggacga cgtctgcgat ttttgttggt
     8701 tttgcttttt ctcctgtcaa acttgtccga gttctgcggt ccgttgtatc ttgttcgatt
     8761 tcttcgacca tcgtattcca ttttccgcaa tttgggcagc gtcccaagaa cttaggcgac
     8821 acatacccgc aattttggca aacaaattgt acttttgatt tttttgccat tgtaccgcct
     8881 ccttttatcc taatcatgag acattaaatc aagcttaagt atctttaacg ttttatttac
     8941 tggaagaacc aaatccgcct tgacgttgag cagtcgtttg atcttgatct gcttttaaaa
     9001 acggcaaaaa gatcccttgg ccgatacgct cgcctttttt gatcactaca tccttgaaac
     9061 caaagttggt gaattggaac atgatatggc cttcatttgc ctcattattg taataatcac
     9121 tgtcaatcac acctactccg tttgtcaaca ctaaaaaccg cttcaacgga ttgcttgaac
     9181 ggttagcaag ctgcaaaaat tcatcttctc ccatgtaagc tttgattcca gtaggcacga
     9241 gggttggttt caattgtttt tcattttctg atacatcttt tttcaatcca tctgcgactg
     9301 tttccaaccc ctttttccag cctgctttcc agatacttgg aatcacgacc tcttctgctg
     9361 cttcaaaatc atagccagca gcatgatgtg tagcgcgctg cggtagattg atattttgat
     9421 ctttatattt agaaataact tcaaatcctc tttttctcac cggttttcct ccctaaataa
     9481 actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa ttcttcaaga
     9541 aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac tgcaagttgt
     9601 acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata gaacgaaatt
     9661 aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat gcaaaacgaa
     9721 ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc aatcttgcgg
     9781 caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag gaagtgatct
     9841 tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca gaacgtccga
     9901 ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg atgaatatct
     9961 ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg acaagagacg
    10021 tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag cttttgatta
    10081 tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa ttagatcatc
    10141 tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt gtcgcggcag
    10201 accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg actacaaatc
    10261 ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg gcacaagcag
    10321 gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa gccgcttcta
    10381 ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg gcaattattt
    10441 ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg aaatgactga
    10501 cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac ttatgcctac
    10561 aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga atcaacaagc
    10621 gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata tcagtgaaac
    10681 gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta cagagatcga
    10741 aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga cgaacaccga
    10801 cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg atcggcatca tttacgaatc
    10861 tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga atgccgttat
    10921 ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat tattgcagca
    10981 agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg atacttctca
    11041 tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga ttcctcgagg
    11101 cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca ttgaaacagg
    11161 tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg cagtgaacat
    11221 catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa cattgctgat
    11281 ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg tagaacacgg
    11341 ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg ctttagcaga
    11401 agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag tggtcgattc
    11461 tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag aaacgatcgt
    11521 gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg cggctgtcta
    11581 tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg cagagattgg
    11641 tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat taacttccac
    11701 aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt gaaacattat
    11761 tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg tcaatatgtt
    11821 tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg cttttctggg
    11881 agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga gtcaacggga
    11941 aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac gcttgcacat
    12001 tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt ttttcgatca
    12061 ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat cttataaata
    12121 tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg tacgataagt
    12181 agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg acaaagattt
    12241 tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg gtaaatctgt
    12301 ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat aacgaagcag
    12361 ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt cagccagtag
    12421 ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt ttctagtttc
    12481 accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca accgatcatc
    12541 tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat attcttccag
    12601 catctattat ccccccattc ttttttctta aatgaatttt agcttaccta ctgaaaattt
    12661 tccagtttca ttattttgag aaaaaaaggg aatgtgacaa aaatcgtgtc acatcccctt
    12721 tttccgaata aatggtgttc ttctgccaac tcctcaatca taagtcacaa tattcaacaa
    12781 acatgagaaa ctgttttttg aaattgttcc ttgtgattca gagttaaacg acagtctcac
    12841 aacctcttgg taaacggcgt tcaatcagct gaccctcggt attagctcaa aaaacggtaa
    12901 aatatgagaa gctatttttc cgttttgttt ctctaatact taggataaaa cgctgatttt
    12961 acaacctctg ttaaacggtg ttcaatcagc tgtccttcgg tattagctca aaaaacggca
    13021 aaatatgagg aactattttt ccgtttgttt ctctaatact caggacaaaa cgctgatttt
    13081 acaacctcct gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac
    13141 gaaaaatatg agaagctatt tttgtttggc tgttcttatg acttgatgca ggacagcttt
    13201 ttcacaacct ctgatttctt attcttcttc atttgtagca aaataattac ggtgataaat
    13261 gatacaacat acactgataa gaacaatggc acctgcaaaa taaaacgcat agctttcacc
    13321 aatatgttca ccaatcacac ctgcaataaa tggtgctaaa gctccaccga aaaagcggac
    13381 aaaactatag ctggacgaag caacgtttcg ttgcagacct ggtatttcca tagcaatcgt
    13441 cgtcaacagt gtattgataa ttccttggaa aaatcctgct aacacaatgc tgatcggtac
    13501 aaggactggc atagaagttc cagctcctaa taacagcaaa caaataatga ataagacaaa
    13561 tgctgctaaa attgtccgat aagtcgtaaa cttccgctca agagccggtg caacgaaaat
    13621 cgaagcaata gccagaagca caccccagcc aaaaaataca aaaccgactt ctaattcaga
    13681 atagccaatc aataaaaatg gcgcatacgc aagtaaagta aagaatccga aattatataa
    13741 caatgcgatt agtccaactg aacgaagttt atgattgctt aacgctttga caccttccca
    13801 aaggcctact tttctctttg gtttctcagt ttcaggtaaa agtaccgcta gggctataaa
    13861 accgcaaaac atcaaaaatg ctaccccaaa aaatggataa cgccatgaac cactgcccaa
    13921 gaccccaccg actagagggc caacagccat tccgcagcca agtgctgctt catacatcat
    13981 gattgctttt tctgtgttac caactaaaat actgacgatc gttgctaaag cagtagagac
    14041 aaataaagca ttcccgacac cccagccggc acgtaaccca atcagcattc cgatattccc
    14101 agataatccg ccaagaccag cgaatacaac gatgataaac aatcctgtca tcaatgtttt
    14161 tttcaaaccg atccgactag ataagaaacc tgtaaatagc atcaccacac cagtgactaa
    14221 catgtaacta gtaaataaca gggtcgtttc agctggcgta gcgtttaact gtgcagcaat
    14281 cgtttttaag attgggtcta ctagacccac tcccataaaa gcgatcatac aggtagctgc
    14341 tacaactagc gctgaacttt tcccctttgt ttcttcattt gtttgttctt gtatatctat
    14401 ttgattctcc aatatttcca tccctttctc tctattagca tcatacatga tttattttac
    14461 atgtcaatat gcacatgtaa aacgtggaaa ctttactgtg atataataaa gaaaataaga
    14521 cgagaggtag gatttttatg aatactttag cctatatatt attatgcgtg cttttatcca
    14581 aaccacgttc tgggtatgaa ttgaagcagc tgatcacaat tttttgggaa gcccatcaca
    14641 gtcagatcta tacaacgctt gccaagttag accagcaggg ttatgtgacc gttttggaca
    14701 aagacgagca ctctcaaaaa aagatctacc acttgactga tgaaggacgt gctcttgtag
    14761 aggaatggat caaagaggaa actcccgctc ctacgcaaaa agatgaattt ctagcaaaaa
    14821 tctatgcttt ttcgacgctc gataaaacaa cagctactgg actgctattc actagagaac
    14881 aacagctgaa caaaatcatg caaaaaaatc aaaaaaaatt gaatgacctc tcgctagaca
    14941 agaaacaaga atttggacgt tatgttgtga tccagcgcag aattatgctt tgtcagcagg
    15001 aactccaatg gtgtcagcaa gtaagagaac aaatgcagca gttttttgag taagggataa
    15061 acagaacctt tctagaaata tctattgaag agcaaattat cttcaataaa agagcaatac
    15121 agttggaaaa gaaaaagcga tccgaacgac aggccaaaac ctatcgttcg gatcactatt
    15181 tttctagaac tgcattcaag agactatgac aaaaagcatg ccaaggcctt ttttcaaata
    15241 acagtgttcc atcattcatt actttgaatt gcttttagca cacttcttga aagcgggcca
    15301 acgatgatca actgcaacgg taaagcggca atcacattca ttttccaagc attcatataa
    15361 gcagagaata attgatcagg tattccttgt tccatgatca caccgaataa agacatacat
    15421 gtcaccattc ctaaaaccat caagcatgaa atagttaaga tcatttggac cttgctttct
    15481 ggatttactg gtagtttaaa ggcaattttt ttcgccagca caccgatgat gaagacatcc
    15541 aaaatgaaag ccaccacgaa tccaggcact agaccaatca acaaacttga taaatgaaga
    15601 tcttgatgca gccataaatt atacgcgctc atccccaaga ccatcaaaaa acacatcatt
    15661 gttgtaaaaa ttattccttc ttttttattt gtaggcaatc tgtaccactc ctttaaattt
    15721 gtcacgaaaa tcatcgtagc acatttaaaa atttctcgta agtacttttt ttattttctg
    15781 cccttttatt tcataaactt catcagccgt acgctcgaca aagtaagaat catgagaagt
    15841 aaacaaaatc gttcccggat atatttgcat tagcttctct aaagcttcga tcgttgccat
    15901 atcaataaaa ttagtcggtt catctaacag caacacatta cttggcttag taaaaagcaa
    15961 agcaatcgtc agacgggtcg cttctccccc acttaatgta cataacggtt tcgtcaaagt
    16021 ttcctgagca aaccctaagt gattcagcaa actacggaca agcgattctg aaaactctgt
    16081 ttgtctcatg acaaaacgaa tgatggattc ttcagatgtc atttgataac ccttttgctg
    16141 atatacttga aagcttactt tcggagagag aaggattcct tctccttgtt tgcggatatg
    16201 ttctaataag gttgtcttac ctgaaccatt ttcaccgaca atcgcgattt ttttacctaa
    16261 agaaaactga aaatcaccat ttaccagtaa tgttctccca cttctttcca attggacatt
    16321 ttgtcccatg atgggatagc ggctgtggag ttcaagagat tttggtactg gaaaacggat
    16381 ttgtttcatc tgctgcggct tggttgtttc tcctattttt tggagtcttc tctctaatgt
    16441 tttcgcattc ttttggatgg ctttttgtac actgtctttt tgtttagagg aagacaaccg
    16501 atccagtctg atttgttgct ttttcttttt actggacact ttttccaact tttccgcttg
    16561 ttttttcttt tcttggatcg atttttccaa tcgttttttc tctttcatga tcgactctgc
    16621 ttctcgtagc tgagtctttt tctccaattc cttttgagtg agataggcac tataattccc
    16681 cgaaaactct cggatttctc cgtcttttac ttcccagatt ttcgatgcta gctcatctag
    16741 aaaatagcga tcatgactaa caaaaaccag cgtaccataa taataacgaa gctcttcaat
    16801 cagtcgatca atactttttc tatctagatg ggttgttggc tcgtctagaa gcatcccttg
    16861 ttcataagaa gaaagaagac gtatcaactc gatctttcgt tcttcccctc cgctccattt
    16921 ccgctctgaa gtttcttcca gtcttaactg gaaatgactg gctaattcca aatttaggtc
    16981 ctttgcttca gcgagatagg ttaattgagg caggtagttg aaggtgatct ctgtttggat
    17041 tttcccatga tcgggaaaaa gttcaccggc tattagtttg agcaaagtcg atttaccgac
    17101 accattttct cctatgatcc ctatacgatc ttgctgatag acacgaagag aatcaataga
    17161 tagttctagc tggttgccaa agctgacttg tagatttgtt atatttactg ctaaattttc
    17221 catatgtttt acactcctat ttcatgtaaa acaacaatta tcgatactaa aaaagagcag
    17281 aaaaatctgt ttgcaaaaaa actgcatcac atcttttcct actctgaacg aaataactat
    17341 gactaaatca ccaaatctgt catagctttc acctattcta ctgtcgttcg aaaaaatgga
    17401 catactccat ccatgaaatt gttgtttata catgtctttt gaatttattt agttgttatt
    17461 caaaagttcg aaacgtaatt tcatcgatgc agtcattttt ccgacctcct tttttattag
    17521 gttaccacac acgattcaaa aaaagcaata gctactgaat agtaactgaa aaccttttat
    17581 tcgaacatag tgatcttttc tggtgctaaa cgaacagact cgtaaccttc ttctaccgct
    17641 tttcctattg ataaaataat aactggaaca taacggtctt tatccaaacc aaatgcctct
    17701 gccaactgat ctgcttcgaa tccaccaatt ggatttgttt catagccatg atcacgcgca
    17761 actaacatga attgcatagc cgctaagcta gaatcgactt tgactacatc gttcatttct
    17821 tctcttgaaa aactcttgta ataaggaatg atcgcagcta attgttgatc tctgacttct
    17881 tttggcatct tccctgattc gtaagcttgg ttgtagattt cttcgccata ttcgtaacat
    17941 tgcatatcgc caaaaatcat gaccattgca gaagaagtat cattttgtct cgtattgaaa
    18001 cggatcaaag gacgcaagat ttcttttgct tcctcgcttt cagcgaccac aaagcgccat
    18061 ggtttcatat tgacagaaga aggtgcagtc gtcgcttctt tgatcatttc cagcatttct
    18121 tcgtgtggga ttttgtatgt ttcatcatat acgcgaacgg atcttctacc agtcataatt
    18181 gttgaaaaat catttttttg ttccattatt attaacctct ttcgtttctg ttgttatatt
    18241 gcaaagttgg gtattcaatt tatctaaaag tgttagaaat tgtttcattt ctacatcgct
    18301 gaaagccggg gatagcacgg aacctgtatc tttatggtcc atttcacaat gcttgatttt
    18361 cttctgggct ttttctgtta gcgctacata cacttcccgg ttgttctgag gatttctctg
    18421 acgcgaaaca tagccatttg cttccaatac cttcagatga cgagtaactg ctccttgatc
    18481 aatctgcaac ttttcttgca aactgctttg tagcaatgga ttttcttctt tcagtctcat
    18541 catgatctcg tatctagtaa gacttatacc gatattttct tcaaagatct ggacgatttt
    18601 ttgatcggct aatttgattt gatataaaag gtgactaatc tcttctaaag acataatttc
    18661 ctcctttcta ataattgact cgtcaacgat tgacttgtca ataatagaat aaaaaaagaa
    18721 aaaagtcaaa gaatcagctg tgtaataaaa aaaacgctct caatagaaaa gtatagagaa
    18781 aagaaatttt ttgtagaatg aacggaaaaa atgataaaga aagcacaacg ctaaaaccaa
    18841 gattattgat tcttatcaaa ctatagaaga tagctcttct gcagtagata attttacacc
    18901 gccaaaaatt tccgaaatcg ccttttgcaa gaatcccgtc gaatattaga aattggatca
    18961 gtatgcttaa acagtaataa aaaagttatg acaagatgtt gtcacaacct tttttgagta
    19021 aacggtgttc aaaaacttgc atctgcggta ataagttcaa ccaaacaaaa aacatgaaaa
    19081 tctgtttttg tttggctgtt cttactactt gatgcagggc agctttttca ctacctcttt
    19141 ttaataacca taaaatccta ttccttcata tctccagccc atcggtgtta gccaatctct
    19201 ttccttagca ctcaaggtat agtgatgggt accagttttt tcatttggat taaatagtcg
    19261 ataaattggc ttggaaccac cagaataaaa acttagacct tcataacgcc aaccgatttt
    19321 tgttaaagag ttgatctcag attgtgccat tgtgtaatga tggtctccac tgtaaggatt
    19381 gtataaacgg tagacaggtt gtccgctgtt tggtgcttgc caagcaatcc cttcatattc
    19441 ccagcctaca gttggcagat aatttttttc tccggcattc aatgtatata aatgttctcc
    19501 tgtatgacgg ttatacagac gatacatcgg agaagttgaa gttggtgctg tatcatactg
    19561 tgtcagatta taggtttcga ttacattgtt caatgtagaa ccataattag gcgcagtggc
    19621 ataacgacct gttagccaag ccgtagcatc tttgtaagag ttcgtattgc ttttccatgc
    19681 accggaatag tagtaaacgc ctggctgaaa agaagtcgtc ttcaataccg ttacgttatc
    19741 ttgcaatgat tctttgtacg aaggatattt tctaaaagcg gcattcaccg taatccattg
    19801 cccattcaca tattccgaag taggcatcgt aacagattgg ccattatagc ttcctttaat
    19861 cccaaataga ttgtaattag gggcagaagc caaagtactg ttcccccagc cactttcaac
    19921 gatggcttga gcaatcatga ctgatgcata taagtcattg gcacttgcca aactttgtgc
    19981 agttgaggca atactatcaa tgaacgcttg ttgagaatta gcagatgagc gagcataaaa
    20041 agcatagcca gtatagtttt cgaattcaga caaatctgct tgatgggtat gtgtcaatgt
    20101 ttgcccatct gtcaattggt tcgtgatttc ttctgcattc tctggattgt tctgagcttc
    20161 tgctgatgta ctgtcttcgg tttgtgtttg ttctgttgtt tcagttgttg gttcttcggt
    20221 tgaaattgta ttttctgaga cagtagctga ctgcaaagta ctttctgtat cgttttcaat
    20281 tcctgtttct gaagaactcg atgtctgagt tgcgacagtt tctgtcgatt cttcagctag
    20341 cactgttgtg ttggcaactg ctagatttcc taaaagaata acgctaaata ataaattctt
    20401 tgctttcatc ttttttctcc tttgaattat gaaacttttc ctaaataaca gtgtaactaa
    20461 aaaagtaaaa gcaagtcaat gtccatattt aaataacatt tgtttctttt taataaattt
    20521 gctttttctg cataaaaaga cgtaaatgaa gaatattctt ctccattcac gccttaaatt
    20581 gatggtcgtt tgaataacta tttaacaagt aattctctct ggatattatc cgttatggat
    20641 tatacttcct gtgtcgtcgg acggataatc atgttgctga cagacatccg gtctggcgtt
    20701 tctatcgcaa atgctacagc agaagctata tcttcagatg ttaatcccca ttctttttgc
    20761 gcttcatgaa gttcaagagc agcctttttg tcgctgatgg tttgatataa ttcagtttga
    20821 acagctcctg gagagatgat cgtagatttg atgttatttt gccgttgctc ttgacgtaat
    20881 ccttccatga ttgcacggac agcaaatttt gttccgcaat aaacggcgga atcaggataa
    20941 acaacatgtc ccgccacaga atcagttgaa atgatatgcc cagatttttg tttttccatg
    21001 attggaagta cggcggaaat tccatttaac acacccatga tgttgatatc aagcatattt
    21061 tgccattcat cacggtgacc ttcaactaat ggagcggttg gcatgattcc tgcattattg
    21121 tacaatacat cgattcgacc gaacttttcc atcgctaaat cgataactgc ttggacttgt
    21181 gcaaaatctc ttacatctgc tgtttgaata tatagctcag cttctggtaa agattctttt
    21241 attgctttta ggcgatcttc tcggcgtgca gctataacta gtttagcccc tttttctgct
    21301 aatagcttcg ttgttgcttc accgattccg cttgaagcgc ccatgatgac aatcactttt
    21361 tctgataaat cattcattct attctcctcc tgttttgaaa aatctcttgc ttatactcac
    21421 tatctcttat ttcatcttac tgttcaaata atccgttcga caggtttgac gattttggca
    21481 gggattcctg caacgatggt attgtctggg acatctttcg taacagttgc attcgctgca
    21541 acaatggcat tctcgccaat cgttacgcct ggcagaatag ttgctcctgc tccgatccaa
    21601 gcatttttct tgatgtggat tggctttact gacaagcctc ttcgttcttt tggatcggtt
    21661 agatggttca ccgtgatgag gttgacacga ggaccaatca atactgaatc ttctaaacag
    21721 atgccaccta agtcaacgaa catcacttga cgattgataa agacattttt ccctatacta
    21781 atatgtttgc cgaaatctgt ataaaaaggc agcagaacag tcaccgtatc atctactttg
    21841 tcactaatga tattttctag ccgtttgcgg atatctgctg gttcatggta cccagtattc
    21901 agcgctgcta aaggctcttc gttttccgct tggatctgat ggatcacttc aaagagcgga
    21961 tccgttactc gaatctcttt attgacgatt tgtttttcga tgaggcttgt cattttgctc
    22021 cctcctttta tccttacttt ataacggacg gcttatgatg tctaatactt ataaaacata
    22081 cttaactatt ccttttaact atacttagtg gtatgctttt ttaaaaacag gagggaaaaa
    22141 atggatattc gtatactgac gtatttttta gcagtagcta gagaaaaaaa tatcagccga
    22201 gcagcaaaat ctctgcatat cacgcaacct actctctcaa aacaactgaa agatttagaa
    22261 gaagaacttg gtgtgacact tttcactcga ggctcccgag agatacagtt gacggattct
    22321 ggtcgatatc tttattcaaa aggaaaagaa atcttgtcgt tggtagaaaa aacggcgaac
    22381 aatttaacaa atgacgcaat catctctgga gaaatctaca ttggcggagg cgaaactaaa
    22441 gcaatctctt tcatttctga tagccttcat tcgttgatca aggcgcatcc tgatatccag
    22501 tttcatttat atagcgggaa tgcagatgat atcatagaca aactggatag cggattactt
    22561 gattttggac tcgtaatcga accgacaaac aaacaaaaat accagtcatt tcgactgcca
    22621 gcccaagatc gttggggact tttgatacat cggaatcatc ctttagcaaa gcaaacagtc
    22681 atccatccgc aggaactgaa aactgttcct ttgatcattt cccggcaaac gtcagtggac
    22741 agtcaactag ctgaatggct aggtttcagc atagatcagt tatcgatcgt cggaacatat
    22801 aacttattgt acaatgcttc tttgatggtc gataacggga cttttggtgc attagctcta
    22861 gatgggatca tcaatactta tgggacggaa aaagtattca tcccactcga tccgccatta
    22921 caagcttcac tgaatctcat ttggaaaaaa gaccagcctc tttctcctgc tgctcgtaaa
    22981 tttttagatg tcctaacgga gaaatacagt taagtagagt ttttttcctt cgctttcagc
    23041 tttttaacaa cgaataagtt aagcaaaaga tccaatgatt aaataacata agtttcttaa
    23101 tttgttcctt ccttttattt ctgataattc cagtaaatta ctataattat aaaaaaagaa
    23161 ttgacaaaac ataactgtct atataaaatt gattacgtta acaataattc gtttcatgtg
    23221 actagatcat actaggcatg aaaaatctct gaggaacatg gtttccctag gaggtttttc
    23281 gtgtctattt ttgtttggcc gcaatcaact ggtctagcaa acgtgaaaac caaatcttca
    23341 aaaagggtgc gcgcatgaaa atcaaaaaga tctttaatca aaatgcagta ttggtagatg
    23401 acggtggaca ggaaaaagta gctattggaa aagggattgg atttgacaga aaaagaaatg
    23461 atttgatttt tgatcgtgat gtcgaacgga tatttgtcat ggaaccagaa ggtcaaatca
    23521 agttgcaaaa tttacttaat cagattgatg aacgcttttt atttgcagca gaaacgatca
    23581 ttgatcatgc ggaaactgtt ttgatggaaa agttgaatga acatgtattg atcgctttga
    23641 ccgaccacat tgctttttct gcggagaaca ttaataacgg tatcgtcatc cgcaacaaac
    23701 ttttaagaga aatcgaagtg ctttacagtg atgaattcag tattgctcaa tgggcagtag
    23761 agtatctgac aaaagagctt gggattccat atacttatga cgaagcagga tatatcgcaa
    23821 tccatattca cagcggacga agcggtcgga acaataacca tcgcagtatc cgtgaagtga
    23881 cgattgtatc tgacatcatc catttagtgg aatcagaatt ggaaacagat atccattcag
    23941 aaccattttc actgaattat tcaagactgg tgaatcattt acgactgctt ctacaacgaa
    24001 cccatgcgca acaatatgct gttttagaca cggaaatcgt agaaatggtc aaacgaaaat
    24061 acccagaaag ctataagatt tctaaaaaaa tccgtgtgtt gctgaccaaa gaataccagc
    24121 tcgcgatcac gaaagaagaa ctgggttact tggccatcca tatcgagcgt ttaagaagcg
    24181 caatcgtaca aacaaatgtt aacaatcatg aggaggaaaa aaattgaaag catatctaca
    24241 acgaatggga cgttcccttc aacttccagt tgcggtctta cctgctgctg ctttactagt
    24301 agggatcgga aactggtggg catcttatag taacgacatt gtcgctcatt ttttacaagc
    24361 tggcggtaac gcagttttag gtcaattacc tttgctgttt gccgttgggt tagcactagg
    24421 gatgtcagtg gacaaatcag gtgctgctgc cttggcaggt ttggtcgcat ttgaagttcc
    24481 agtaaatgtt ttaaaaactg attcagtagc tactttattg aatatcaaac cagaatcagt
    24541 tgatccatct tttgctcaga tcagtaacgt atttattggt attattgccg gattgattgc
    24601 ggcagctctt tataatcgtt tccacgaagc aaaattaccg atggctttat ctttctttag
    24661 cggcaagcgt ctagtgccaa tcttggctgc ctttgtcatg ttgctgatca gtgcagtgct
    24721 actgctcgta tggcctccgg tttactctgc tttagtttct tttgctaaat tcattttgag
    24781 tttaggctgg ggtggtgcag gtctttatgg cttcttcaac cgtttactga tccctacagg
    24841 attgcatcaa gcaatgaact ccgtgttctg gtttgacgta gcaggaatca atgatattgg
    24901 taaatttttg gcttctgaag gaacaaaagg cgtaacagga atgtatcaag ctggattttt
    24961 cccaatcatg atgttcgggc ttccagcagg agcatatgct atttacagaa atgcacgtcc
    25021 agaaaacaaa gcaaaaacag cttccttgat gttagcagca gcttttgctt cattctttac
    25081 tggtgtaaca gaaccacttg aattctcatt tatgttcgtt gcttggccat tatatgtctt
    25141 gcatgcaatt ttcactggga tttctttagc agtgagtgca ttcttccatt ggacagcagg
    25201 atttgccttt agtgccggat ttgtcgatta cttcctatct ttgaaaaatc cgattgcgaa
    25261 tcagccattg atgttgatcg tccaaggatt agtaacagca gtgatttact actttggctt
    25321 taactttgct atcaaaaaat tccatctgat gactcctggc cgcgaggaag cagatctttc
    25381 agatgacgat actgcaacga ctaattcaga caataaatat gcggcacaag caagcaaaat
    25441 ctacgctgca ttaggcggag ccgacaatgt aacttcgatc gataactgta ctacacgcct
    25501 tcgtttgcaa gtcaaagata caggcctgat cgatcaaaat aaaatcaaag ccactggcgt
    25561 acctggtatg aagatcatcg acggaaaaaa tgcgcaagtc atcgtaggta cagaagtaca
    25621 attcgttgca gatgagatgg caaaactaca tggtggtgct gcaacacgac ctgcacaaac
    25681 aaacaccgtg gttaaaacag aaacatttgc cggtggaacg atcgaacaag atatttacgc
    25741 tatcgcaaat gggaaactga ttccaatcac tgaagtttca gatgatgtat tcgcggaaaa
    25801 aatgatgggt gacggctatg ctgtattacc agaaaatgga gaaatcttct ctccaatcgc
    25861 tggtacgatc acaaacattt tcccaacgaa acatgctgtc ggaatccaaa cagatgctgg
    25921 tatcgaagtc ttgcttcata tgggcatcaa cactgttgac ttaaaaggcg aaccattcac
    25981 tttatatgta aaagaaggtc aaaaagtcgc aagaggtcaa ttgattgctt tagttgatct
    26041 tgccgctatt caatctgcag gcaaaaacac ggatatggtc gttgtattca caaatggcga
    26101 caaagtccaa agcttagaaa tcgaacccgc acgtgatgta aaagcaaatg ataaaatcgg
    26161 ctctgtttcc aacaaagcct gagccatcgt cttccccaga cttttttatc gataaacaaa
    26221 caggagctga gacaaaaatc ttgtctcagc tcctttttcc gaataaaaac ggcgttctcc
    26281 tgccaactcc gcggtaataa gtccagccaa acgtaacata tgaagagcta ttttttattt
    26341 gactcttctt attacttgat gcagaacagc ttttttacaa cctctgataa acggtgttca
    26401 aaaaccagtt tatcggtatg aagtcaaatt tatgcaaaaa tgtggaaagc cattttcaca
    26461 aaatttttct tcatactcaa aactaatcgg ctttttcaca acctctttat acattattcc
    26521 tgatccaata tttcttttaa tgctgcttca agagaagtta atggttttcc caatactttt
    26581 tctaaatcat cagaggaaat agctaactga tcctttttaa tatcatgttg gatcgctaaa
    26641 aacatgtcag ctgcttcttg agggaatccg gcttctttta gattttcgac aaacgctgca
    26701 tcacttgctt caataatttc taacttttta cctgtcgcac gctctaatgc cttggctaat
    26761 tcttcatagt cagttagatt gccggataat tctaagattt ctggagagtc actttctact
    26821 agtacttttg ctgcagcttc tgcatattca cgtttcaatg cccaacctgt ttttccattt
    26881 cctgcagcat aaacgaattg accagttttt aaagcctgcc caatgatagg taattcattc
    26941 tctacatacc aattattccg taaaaatgta tgggaaatac ctgaatcctt aatcattttt
    27001 tcagtgaatt ggtgatctgg cgctaataca ctgatcgagt gatcggcatc tgcaaaactt
    27061 gtataagcga tataagatac cccagctgct tgtgctgcct tcactacatt cttatgttcg
    27121 gcttgacgat ttcctggaac gccagagaca aaaagcaatc gatctattcc tttcaacgct
    27181 tctttcattg agtctaaatc atcataatca ccaatacgta tattaaatcc agcttctttc
    27241 agtgcttctc ctttttcttc actacgtact agtacataga tttcattttg tggaaccatc
    27301 ttttttaaga attccagcgc ataaccacca aaacctccag ttgctcctgt tactaaataa
    27361 tgcatatttg ccactcccta ttctttttat ttgttaagat ttacagctgt acatttataa
    27421 attacacctt tatcataaag agtgtggtaa gataagtcaa gcaaaaaaaa tgaaaggatg
    27481 acaactgatg aagtattctc tgcaatttag tgatgcaatc catgtacttg cctatattga
    27541 aatgtttcaa gatacccgat cactttctag tgagatgatt gccagtagta tcgaaacaaa
    27601 tgcagcaaat gtacgtaaaa tcatgagtca gctcaaaaaa tccggtttga tctttacaaa
    27661 aaccggaaaa ccgcagcctt cccttgccaa aagtccagat cagatttctt tactagatgt
    27721 ctatcgcagc atcgaaggta atacacagct tatccaagtc gatccaaaaa caaatccaaa
    27781 ttgcgtcgtt ggtgcgaata tccaagaagc tttagaagaa agctacgctc ggctgcagaa
    27841 aaaggcagaa gaagaaatgg ctaagatcac acttgatagt ctgatacata aaatagccgt
    27901 tttggaaaaa gaaaaaagac cagaaaacac aggcctcgta gagagatttt tataattctc
    27961 tctactgtgc ctgcattttt ctgatcggaa tcgtctacat aaattctttt attctccggg
    28021 ggacaaaagc cttgtcatgc ccctttttcc tctctaatgc tcaggtcaaa gcgctgaagt
    28081 catgacgtct tgataaacgg tgttcaagta gctgaccctc ggcattagtt ctgaaaaatg
    28141 caaaatatgg aaagctattt cccatttttc ctctctaatg ctcaggtcaa agcgctactt
    28201 tcacgacctc tatattatca tggtttattg actaccggta tccagatttc tcccatatat
    28261 ttcccgttgg taactcccat ttcgactaca gcatttggac ccccaacata ggcgtaatct
    28321 tctacttctc ttaaaacatg cccaaaggcc gtattcgtca aatcatttct taattcttca
    28381 ggcgattctg ctaaagcgat tacaacaaga tacgttcctt ttgggaatgg gatgattcgg
    28441 gtagtgtttg gaacatcctg actcgtttga acaccgacat agtacatcat tttcccgtta
    28501 tagggttcgt tgactgtaaa gaaataatga ttttcagcag cttgtttcaa tgcatctatt
    28561 gttccattac cgataacttt ttgctcaaat gcttgtttct ctgcagccaa accgacaaaa
    28621 tccgtattgc tactcttcaa ttccacacca atacccgtca ccgtaaaatc tggtttttct
    28681 tcgatcctat actttgccat ttctcccacc ctttctatta tattaagttc atcttacata
    28741 aaattaaagg gaaatgacac gattacgctc ttttttctag ggatattttt attttttaac
    28801 tatttttttg ctttcactgc aatccaaatc tcactgtaaa cattactgaa atcctctccc
    28861 cattttgtaa aagagatctc gggagcttcg actaactcat aatctgaaga aggaagccat
    28921 tctgcataga ttttccccca agtatcttgt aaagtttggg gaaatggccc tttattagga
    28981 aaaatggccc aagtgtgttc ttctatagca agcatttcta aatcagcgta cggattttct
    29041 tttgtcgtag caaagccaat catgtgcatc aaactgccct tttcttccaa gcgggctcca
    29101 tcaaatccat aagaaacgtt gattacttga tttggatata aatctcctaa cttatgcatc
    29161 tcttcccttt gagtctccgt gatcgtctgc gctaactttt gtatttcttg attgactcct
    29221 tcaaatttga caggaacttt tttcgccaca cctgccacat aaaactgttc tttcttttcg
    29281 atacgaaatt ccatcgattt tcctcctctg acatcaataa aaaatgtcaa gcgtggaaaa
    29341 gatttttgta tgccgtatct gctgatttct gaaggcagat atcctgacca atcacgaaaa
    29401 gctcttgaaa aaccttcgac tgtttcatat ccatatctaa acgccgtttc tgtcactctt
    29461 gatccatcta ggagatcgcg attagccaaa gccaatctac ggtttttgat atagtccatt
    29521 agcgacatcc ctgcaataaa cgtaaagatg cgttttaatt gatattcaga gattcctgta
    29581 ttttcggcta ttattttata ggaaagtcct tctgtcaaat gttcttcgat gtaatcgatc
    29641 aattggttca gatctttaag catatttatc ttcctccttg cttgcctttt tatcatatta
    29701 tcaaagaaag tcaattactt gatgaatcag gaaccaattt agtcagatgt agaaaatgtt
    29761 gtatggatct aaagaaaatt ctcaagcagc gtttcgtaac ggataattgg tattatctgc
    29821 ctatctatgt tttaccttta gaaaaataaa ttttggggaa gacacattct aagtaaacaa
    29881 agcggtaaaa atatctttta ttcccttagt taatccgctt actacctact gtacaaagca
    29941 aattgttgtt caaactgctg atcagaacgc tgcttggatt tttctttgcg atacatcata
    30001 cctcttttat caattggaac aattatttat ttctaacaaa atcacttcat ttttgtcaga
    30061 taaataaaaa acaaaaaaag ttccatcttt aaaacacgct attcttttac tttttctcac
    30121 attctctaag aaaagaaaag gagaatgaat ttttgacaat tcgagcaaaa gtgattatca
    30181 aatatgtaaa atcagctact tttttcttaa acgtttcttt atactaaata taaataagtg
    30241 tttcatgaaa ggagaactag atgaataatt ggcttgagta ttttcctgaa aacgttttgg
    30301 aacgcggtta cagctatcat ctgcacggtt ttgtccgtca tttgaactat acaagcaagt
    30361 acttgtccgc aacagtttct ggcacggaag attataaggt agtgatcact tgggatgaaa
    30421 agaccaacat gacttgtgat tgtctatatg ccatagaagg aaaaaaatgc aaacacatgg
    30481 ctgctgtctt attcgcttac gaagaacgtc caataaaaaa atccaactac tcactttccg
    30541 aactctcgtc ccttgtttcc tccgctagtt cctctcttgt acgggagctt ttaacagaga
    30601 ttcttataga acatccgcaa tttatcgaac gcttcaaagt taagatgcca tttcatgcaa
    30661 taaattattc agataaatta acaactatca tccataaata cgatcatatt atcaagaaaa
    30721 acaaaaacag gaaaacagcg aagttcatca tggagatgcg aaagttcatc caagaagcag
    30781 ttgaatcgct cattcagcaa aatgcctatc ttcccgcatt cgaattgatc aatgaagtga
    30841 ttgctactct cgaaacattc tattgggagc ctgaggatga acgaaccctt cttctgatag
    30901 aggactgtta ttatttatgg aaagaattat tagcagaagc acctcatgca gaaaaaaggc
    30961 aaatgttctc ttggttcgtc tgccaagtcg atcatactga cgcctcttat tcaaaacgct
    31021 actccataaa aatcctgaaa gaagactttc gggaaaaaga attttctaac caaaagaaaa
    31081 aaatagataa aaaaactaaa gaaacggtaa agaaagacga tttcaatgaa aaatagcaag
    31141 taaacgataa caaccatcct aaatccacaa gaaatacagc agaccttgct ttaaaaacac
    31201 gaatcatgca aaggatgaag taaagaagac ttaacgcgta cccaatatta gtcatttgac
    31261 tattaggtac gcgttttgtt ttataagtca tgacaaattt ccttcgttgc tgggtagatt
    31321 ttccggcatt gttgatagat tttatgatac ttttctacat tttgtaagag aggttccact
    31381 gcatctgtat attggataaa attctgacaa caactttcag gtgtgtcgaa ccactctaat
    31441 ctttaaagat aaatattaaa ataacgatta ataatattaa cagtctttat ttttcaccac
    31501 caaaaaccta tctttgcata tactaaatag acgtatatac acagataggt ttgtcttgtg
    31561 aaaaataatg attatcctta ccaaaaaatg tttgtttttc tacatgttcg tacttatcaa
    31621 aaaatatgat ccttattgaa attactggct atttatattc caacgacttc ttgtgttaat
    31681 ttttgttatc tcatagtaaa ttatgtttca ttttcactta atttagttgt cacgtgttcg
    31741 tcaattcgta aaaacggtaa atatataaac acacttgcgg ctataattaa tatttgtaaa
    31801 acagctgctc gccagtctcc ggctgttgct aaaaatcccg atataactgg tggcgtagtc
    31861 caaggaattt gcactactac atgattaatt aatcccacag cagttgcaaa atatgctact
    31921 gtcaaagaaa aaatcggtgc aagcacaaag ggaataatca gactgatatt aaatacaata
    31981 ggcaatccaa aaataatagg ttcgcttata ttgaatatag cgggagttac cgataattta
    32041 gcaaagtcct tataatcttt tctacgactg aagataaata ttgcaatcaa taaagcaatc
    32101 gtatttcccg atccacccat taccgcaaag gtatctttaa aggccatatt gataatatgt
    32161 ggaatttgct ctttgtttgc ataagctgcc atattttctt gcatattctg aatcaaaaac
    32221 ggatctaaca atgaacctga tattactgct tgatggatac caaatccaaa aaatagatta
    32281 gcaatagatg taattaacaa aaatcctggt aaaccagttg taattttact taatggctct
    32341 gtaactatcg tggtaattag cttatttata tccatattaa acaattgaat gattataaat
    32401 gacaataaag caaacgaggt aatcgtaata atgattggga ttaatacatt aaaagacttt
    32461 aaaacagcgg gaggaatatt tccggatagc tgaatctgta gtttcttatt attagaaagt
    32521 ttaataaata attctgtagc cgctaatcca acaaatatac ctacaaacat tcctgaagct
    32581 cctgtatagg atataggtat cacacccgtc acttcaacag ctttatccgc attttcagga
    32641 gtaaacatca tctgcatagg tgttaataca acaaatgtcg aaatagcaac taataccgct
    32701 gcaatatgat ttgtataatt tctgcttgca cacatatgat aagatactgc tgctacaact
    32761 aataatgtag ttatgcttaa tgtaccattg gcaattgaag taccaatttg ttgtaacgtg
    32821 gtcaatgtac tagtatcgac aatttccccc atgaatcctg ttggttctaa gatcactgaa
    32881 ttaataagtg tcacaaatcc tgctaacatc ataaatggca taaatgtagc aaacgcatct
    32941 cgcaaagttc ttaaatggat ctgattcccc aacttagtcg caactatacc aaatttttca
    33001 aatacaatgt ccttcctgct acttttgccc atattttttc tcctttatgc ttattgtaat
    33061 cgattacttt ttccacccaa ataatacatc acttatcaaa aaaagtaaat acttttaatt
    33121 aaatatatat atctttttta gtataaaaaa ataagctctg aaaaaataat tttcccgagc
    33181 ttatttttca acaaaaacag cttcaaaaac aaagtcttga tataaatgtc tcgataccga
    33241 atactcgaaa ggtttaccag acgatagagc aacaactttg tctatctcaa tcataaattc
    33301 acctggatct atttttaata tttttgcgtc aaattcattc gcagtatctc ctttgatacg
    33361 tatgtgcgaa gtctttattt ccagacccag ttcatttgtg atataagcat atacagattt
    33421 ctttaaatgt tttggttcca agcctggtat gactgctaat ggcatgaatg tttgctcaat
    33481 cgagtatggt tgacaatcaa taaaacggct tcgtacaata ttatatacat agtcttttac
    33541 actgatactt aatttatcag ctactttttt cgatggaagt tcaattgaga aagtatggat
    33601 ttctgaatct acgtgttctt caccaaccac gctaaacaaa ccttcgtttg gaccaagaac
    33661 tcgtgagtta tcaattggtg ttctgacgac ttcagtccca tacccacttc tacttcgcaa
    33721 aactccttcg gaaacaagta agtctacgcc tttctttact gttaacttgc ttgtatgata
    33781 aataactgcc aattcatctc ctgtaggaag tttctcacca attttatact ctccatttac
    33841 aatttttttc tttatatcta agtaaatttc catatacttc gcaatttttt tcaatggtga
    33901 ccctccaatc aaaaacatat cttcatctat actttaacat aaataaaccg ataaaatcca
    33961 catcaaaatt atacacacaa taaaaaagat atatatattt aaacaaaact atttacttta
    34021 agttgtaagc ggtgttatca ttaagtggaa acaggaggta aaaaaattat gacaactatg
    34081 aaaaagctaa cttttccaga tgatttttgg tggggttccg cttggtcagc tgagcaagca
    34141 gaaggaaaag gagaaacagg aaaagcagag actatctggg atcgctggtt taaagaacaa
    34201 ccttaccggt tttatcaacg aatcgattcc tctattacaa ccgatcacat ccatcaatat
    34261 aaagaagata tagaactaat gaaaaaaact gggcataact cctttcgcgt ttctatttct
    34321 tgggcacgaa tgtttccaaa tggcggtacc ggaaaagtaa atcccaaagc catttctttc
    34381 tatcgaaaat tattttccga aatgaataat aacggaatca aagtatttgc caacttatat
    34441 cattttgaca tgcctgctac tcttcaagat aaaggaggat gggaatcacg tgaagtagtt
    34501 gatgcttatg ttcattacgc tgaaacttgt tttaaagaat ttggtgactt agtttgtcat
    34561 tggtttacct tcaatgaacc acttgggcct attttaggta cttatcttga agattttcat
    34621 tatccaaacc tcattgactt taaacgcgga gcgcaagcag cattcttcac tatacttgca
    34681 cacgcaaaag caatacaagt atttaaagaa ctagctcttc ctagtaaaat aggcgttatt
    34741 ctaaatttga gtcctacttt tcctcgtagt caaaatcctg ctgatgtgaa agcagcagaa
    34801 atcgctgatt tattttatac gcggagcttt ttagatccta tggttaaagg gaatttttct
    34861 aaaaagctta tcctgttatt acaagaatat aatcagcttc ctgaagatta tacgaaagaa
    34921 gatcttgtat gtatctacga aaataccgct caaattcttg gactaaacta ttacgagcca
    34981 cgtcgcgtca aatctcgttc ttctatggtg gatacatcta gtccattttt accagagtgg
    35041 tttttcgaac cacatatcat gcctggacga cgaatgaacg aataccgggg atgggaaatt
    35101 tacgaaaaag gaatttatga tttgtgcatg gatatcaaga accattatgg aaacatcgaa
    35161 gcatttatct cggagaacgg aatgggcgtt gcggatgaag aacgtttcat ggatgataca
    35221 gggcaaataa ttgatgatta tcggattgag tatatcaaag atcatcttgc ttacttatgg
    35281 aaggcaataa atgaaggatg taacattaag gggtatcatc tttggacatt tattgattgc
    35341 tggtcatgga tcaacgccta taaaaaccga tatgggctgg tttcactcga ccttactact
    35401 caaaaaagaa caattaaaaa aagtggcgaa ttttacaagc aattaagtga tacaaatgga
    35461 tttagatacg atgtagactt gttagttgaa taatattcta aaattccact tgaagtgaac
    35521 acattagaaa acaagaataa tgagaaatac caataacaaa tactttttct tcatacttca
    35581 tttatacttg tcagtataag agactgtgac aaatgtagtg atcctcagaa gttaggtctg
    35641 gaaatctaac ttgagaagat ttttttaaac tacgtccagt cttttttatt tgtttaaaat
    35701 tgaagttgac aagtcttcat taaaaattta gaatattcac aagtttgacg acaattatga
    35761 aaatttatgt cgtgctaaag gagttttgtt attggatgaa aagttagata taaaaactgc
    35821 gatcaaagac acgttaccta ccgtttttgg ttatatcggt attggacttg catttggtat
    35881 cgttgggaaa gctgccgggt ttcatccgct ggtcgtcacg ttgatgtccc tgctgatcta
    35941 tgctggttct gcccaattta tcacggtcag catgcttgct agccatagtc cactgctctc
    36001 catcgttctc tcgacctttc tagtcaattc ccgaatgatt ctgatgagta tgacgattgc
    36061 tccttatttc aagaaaaatc ggctgcttca aaatctgctg attggcacat tgctgacaga
    36121 tgaaagtttc gcactgggaa tgaataaatt gaactatact ggacaaaagt taaactttcg
    36181 ttggatgaat acagctaatt ggatttctta tttgacgtgg gtgggttctt ctcttgttgg
    36241 cgcactttta gggaatttta ttactgatcc gaaaaagttt ggtttggatt tcgccatcgt
    36301 cgctatgttt atcgggttac tttatcttca agtcatttct gatagaaaca cctctaaacg
    36361 attacagttg atcctgattg gtctcacttt gattctggta tatgtcggac tgatttttat
    36421 cccaagtaac ttagtgattt tagttgtaac actgattggg tgcggattgg gggtgtggat
    36481 caaacatgcc ttcttttgag tttacattgc tgactatttt aggttgtact ttagcaactt
    36541 ggctttctag gatcctacca tttgtcttac tgaaaaaatt cgatttgccg caaccgttgc
    36601 ttgagtattt aagttttgtc ccgatcgtca ttatgtctgc tttatggttc agcagtctat
    36661 ttacccaaaa tatcggacat ctgccgcaga tcaatctcga aaatgctttg gcttcagttc
    36721 ctacactttt agctgctatt ctttcaaaaa gcttattggt gatcgtcttg gtaggaattc
    36781 tttccctttc gttgattcgc ttattataaa taaaaaaaca agagcttatg gcaaattact
    36841 gtcatgcgct cttgttttta tcaaagtcaa ataagaaaaa aatcggcgta ttcttgctta
    36901 tccgttgaac ggaccactaa caacgacttt tcctaatata tgtcctgttt ctacgtcttt
    36961 ggttgcttgt ttcaagttct tcgcattcat cccatctgaa tagaccttgc tcaatgtact
    37021 gcggataatc ccttcatttg ctaaagcagc aatgtgcgct aaagcttctc cttgcgtttc
    37081 gatttcatag ttatagtctg ttttagcgaa catatactcc cagtcgaagc tgatagattg
    37141 gtttttccaa acacctatat ctaacggctc tttgatccct acgatcgttc ccacatgtcc
    37201 aaatggtcgg acaaggtgtt tgatttgatc aaagtagcgg gtaatatcga ataatacagc
    37261 gacatactct acttgatcga ttcctaattc acgaaaactt ttacttaagt cgtgatgata
    37321 atcgattgga tagtctacac ctgttttttt cagccattca aaattcttcg gacttgcagt
    37381 cgcgtagact tctaggccag cccaatgcgc tagttgattc aaaatcgaac cgacgccccc
    37441 agctccgttg atcactaaga tttttttccc gcggttggca ttttcttctg gagtcagacc
    37501 aaatttttca aataacaatt cataagcggt caatgaggtc aacggcaagg ctgcggcttc
    37561 ttcatctgaa agattttttg gtgcaagagc gacaatacgt tcgtcaacca gttggtattc
    37621 ttgattgctt cctgctcttg tcgttgtacc tgcgtaaaaa acgcgatcgc cgacagaaaa
    37681 tttttgcact tcatcaccta cagcaacaac tttcccgact ccatcaaacc ctaagatacg
    37741 taaagcattt cttatcccgt tatcttggcg aagtttcgta tcgacaggat taacagatac
    37801 tgcgttgact tttaccagaa gatcccgtga actgggtaca ggtatctgtt ctttttcatc
    37861 aataaagctt tttggatcat cgatcgacaa cccttcataa aaaccgacta ccttcattaa
    37921 ttccttttct gacatacgaa ttcctcctta gcttcatcaa gttggagcat ccatacagtt
    37981 ttcatttcaa tatatatagt atgctatttg ttccactttt attcatcata attattctct
    38041 tacttaaagt aaactatttg cacttttttg gttaaaaaag aggttgcgac aagacttttg
    38101 tcacaacctc ttttaaacgg tgttcaatca actgaccctc ggtattcgct caaaaaacga
    38161 caaaatatga ggagctattt ttgtttggct gttcttatta cttgatgcag gacagctttt
    38221 tcacgacctc taattcacgg tgttcaagca agaaatcatt gttcactgaa aagcgttgga
    38281 tgttcttttt caaaggtgtc taagatctct cgtacttcat cagtagattt cgtattcatc
    38341 aactgaactc gcaagtcatt tgctcctgga aatcctttga tatagatttt aaagaaacga
    38401 tgtagtcctg tgatggaacg cgggatgatt tctgaataat gatcttgcag atctagttgc
    38461 agacgaagta gtccgatcag ttctttttct gtatgttccc tcggttcttt ttcaaatgca
    38521 taaggattct tgaaaatccc ccggccaatc atgatgccat ctacgccata tttttcggcc
    38581 aattcttctc ctgtctggcg atctgggata tctccgttga tcgtgatcag tgtttgcgga
    38641 gcgatttggt cacgtaaagc aatgatttta gggatgactt cccaatgtgc atctacttta
    38701 ctcatctctt tacgcgtacg caaatggaca gaaagattgg ctatatcttg atgaagtagg
    38761 tgactgatcc attcttccat ctcagacatt tccgtatagc cgatacgcgt cttgacgctg
    38821 acaggcaaac cgccagcttt agccgcttca atcaattgtg ccgctacttc tgggcgaagg
    38881 atcaagccac tgccttttcc acgttctgct acattaggga ctgggcagcc catgttgata
    38941 tcgattcctt ggaagcccat ttctgccata gcaatgctca tttctctaaa aaattctggc
    39001 ttgtctcccc aaatatgggc aaccatcggc tgttcatctt ctgtaaaggt caatcttcct
    39061 cgtacactgt cttttccatc tggatgacag aaactgtctg agtttgtaaa ctcagtaaag
    39121 aagacatccg gtgctgctgc atgctttacg acatggcgaa atacaacatc tgttacatct
    39181 tccattggtg ctaaaacaaa aaaaggcttt ggcagctctt tccaaaaatt agtagtcatt
    39241 tatttgttac tctccattct atcaagcagt gaagtcgcaa tctttctcaa aaacattgtc
    39301 attatactag gaaacaaaaa aagaagcaaa gtgaacagct taaaagatgt ttctgattga
    39361 gcaaaaaaag actgtgacaa aaatcttatc acaattcctt ttccggaata aatggtgttc
    39421 aaaagcttgc atctgcggta ataagtccaa ccaaacgaaa aataggagga gctatttttg
    39481 tttggctgtt cttattactt gatgcagaac agctttttca caacctctcc actaaacggt
    39541 gttcaatcag ctgaccctcg gtattagctc aacaaacgac aaaatatgag gaactatttt
    39601 cccgtttgtt tctctaatac tcaggtcaaa acgctgattt cacaacctct tttctaactt
    39661 ctcaatcctc ttcctttatt gatcaaatac caagtaacgg cgtacaaaac aacgatgaat
    39721 agcacaagta cgatcatcga tgttgctact ggcacatcga ttgttccaag gaatccgtaa
    39781 cggaaaccgg agatcatata aacgatcgga ttgactttcg aaacagcttg ccaaaatggc
    39841 ggcaacatag agatggcata gaaaacgcca cctagataag ttaacggctg caagacaaat
    39901 gtaggcacga tcgatacatc gtcataagac tgagcaaaaa tcccgttgag cagtcctgct
    39961 aacgaaaaca agattgccgt catcaataat gtaatgatga ccatgaacca tgaatacaca
    40021 tgaagtggta caaagaacaa ggaaatgatc gttacgagag agccgacgag aatactgcga
    40081 cccactccgc caatcacaaa gccccaaatg atcacatgcg taggaacagg cgctaccaag
    40141 atttcttcga tgtttttctg gaatttttga gagaagaagg acgaagaaac gttggcatac
    40201 gaactagtga tagcagacat cataatcaaa ccaggcacga tgaattccat atatgaaaaa
    40261 ccacccatgt caccgatccg accaccgatc atttttccga aaataacaaa gtacaaggat
    40321 gtcgtgatca caggcggaac cagtgtttgg acccagatac gcaaatagcg gttcgtttcc
    40381 ttgatagcta aactttttaa tgcagtaaaa taaagattaa acatttttct cctccgtatg
    40441 attattttct tctgtgattt tcaaaaacag ttcttctaaa cgattcgact tattcctcat
    40501 agaaagaact ttgattcctt gtcttgtcag ctgatcgaag attccgttca ccccttgatt
    40561 tcgttcaact tcgacagcga ttgtctgttc atcttcaaat gtacaagcgt atccttcaat
    40621 cacaggtttg gtctcatatg gcgctaaatc aaagataaat gtttcaaatt gcagtttgga
    40681 tagaagctct ttcatactag tattctcgat cagttcacct gattggatga tcccgatgtt
    40741 gcgacaaagc atttcagctt cttctaaata atgcgtcgtc aaaatgatcg ttgtaccatt
    40801 ttcgttcaat tctcggagaa actcccacat ctcgcgtctc aattcaatat cgactcctgc
    40861 tgttggttca tccaagatca gcaaacgtgg ttcatgcatc aatgccctag caatcatcag
    40921 tcttcgcttc attcccccgg aaagcatacg tgcacggaca tttctctttt cccaaagatt
    40981 ggactgcttc aaatattttt cgctgcgttt catcgcttct ttgcgagaga caccgtagta
    41041 gcctgcttga ttcacgacga tttgctggac cgtttcaaag ggattgaaat tgaactcttg
    41101 cgggactaat ccaatctgtt gctttgctcg gactagatcc gtatccagat cataaccgaa
    41161 gacattcact tttccagatg ttttattgac taaagaagta atgattccga tcgttgtaga
    41221 cttcccagct ccatttggtc ctaatagcgc atagaaatct ccttctttca cggaaagatc
    41281 gattcctcgc aatgcttcga ccccacttcc ataaaccttt ttcaaattat ttatttcaag
    41341 cgcatttgcc atttatttcc tatctcctta tagacatgat tctttattgt atcatggaat
    41401 acagcagatg aaaaaacaac ttggttccag cgaaaaatca ggttgctggg ctcaggaaaa
    41461 tctcctaatg aaagacatca atcggacgaa gtcagacaac cttttcagaa aaaacaaatc
    41521 gaagataaag gattactatt catatgtgca acaaaaggag cgtgagacaa aagtaaagaa
    41581 cacttttgtc tcacgctcta aacacgtata aacggcggaa gcaaaagcca agatttttga
    41641 tccaataaat actttcgatc acttcgccta tcccaataca tgccactgcc gcttttcatc
    41701 aaataacgcg gcttccgttt ttgcggtttc tactaaagtc tctgggtaat ccaaaacttt
    41761 cagcaaggca atcgcatttc gggtccttga aggtccttct tttaatttga aatcaaaact
    41821 gattcctttt ccttctgtta cttgttcttc aaagtggaca ttctcacaat aattctttaa
    41881 aatctctgtc aattcgatat catgtgtggc gacaaatgct aaagatggat actccgcaag
    41941 ccaccgaacg actgaagcgg aagaagcaat ccgttcgatc gtatttgtcc cttttaaaat
    42001 ctcatctacg aagcagtagc agcgttcttt ggaagccact tggtccaaca aacgtttgat
    42061 cgatttgatt tctgaaacaa aataactgtc gccttcaaat aaatcatctt cgattgccat
    42121 ggaagtcaac acatgtccag gttgcagggt aaactgttct gcaatcgctg tttggatcgt
    42181 ctgtgctaga atacagctga tggctatact tttgacatac gtagattttc ctgatgcatt
    42241 ggaacccgtg actaatgtat tcttctgcca gttgaccgga ttcactaccg cttcttttaa
    42301 caatggatga tagatatctt tcgctgtcac accgccctca gtaaactctg gtcggcaagt
    42361 gatgggcata taagtacggt aattcaatat tgcagcggct acttctaatt ctcccagcaa
    42421 ttcccacagc ttgatggctt cagttgaatg cttttctaat ttagtgagaa cgaaatgata
    42481 ggagataaat ggcagcatga acatgatgtt gacatattca aaaatcaact ctgcttcact
    42541 cccactcttc atacgaaatg aaaaacctaa tttagggatg tctcttaatg gtttagtttg
    42601 ctgtttcagc tcttcttgaa gtggtgtcgg cagttttgct agctgattgg cggctgaaat
    42661 cgttgaaacc aaataactca tgctattcag ctctgtttct agtgtttgtt tcttcactag
    42721 ataatagacc gtattgaata ctaacccacc gattgccaaa tagattccta gtacttgtcc
    42781 taaaagaagc aaacccaaac ccaccagcgg aagcaagcca agaaaaataa ataaacctac
    42841 gcgtccaatc tttttacttt ttccatccgc tagatattgt tttgcaaaat tatgatcttt
    42901 ttttcctaaa cgagcgaatt gatattggat tttttctctc agttgaggat tttcctgata
    42961 aaaagcaatc agtttttcta actgctgatc ttcaccaaaa tcaaagctgc gtaaacgttg
    43021 atacaacgct tctgaaccta cactagaata ggtactgttg attccttcga atacttcgaa
    43081 catatccagg tcataccaag taagatcatc gatttcgcta tcccatgaac ggaacttctt
    43141 ctccgtgagc catgcttcct tcaagctttc ttctttatca aatctcggtt gataaggaat
    43201 tttttcccat ttgctacgaa ctgtttcctt taatttcaaa cgtgaaaaaa tgtctaaagc
    43261 aactaataaa ataacagctg caataataat taaaacgata aattctcttt gacccatatc
    43321 cctctccgtt cctttatttg ttagctttag tatagcactg ttgcgctatt tgaaaagagg
    43381 gtaaaaaaac acctttcaaa ataaaaggtg tttttttctt gtatattaca tatttatctc
    43441 tcatttatca aaattgacaa ataatcatag ggaggaattg ttgttaattc ttgtttattc
    43501 tccagtgcat tgacaactgt cccaagggaa aggatagcct caggtactat tttttcttgt
    43561 tctgttggat tagctaagcg aagaacaaat ccttgttcat gaaaataaga aggataaatc
    43621 gatgatacaa ttaagtcttc aggtaattca atcaatgaaa atttacgagg aacatcaagt
    43681 tcattttgta atggccaaat cttgttatct aacctgtgaa taaatacatt cagtttctgc
    43741 ttttgataag aaatagattg ttctaaccgt ttttctgcct gttttgccac tttgaactca
    43801 ttaaatcttc cttcttctac attaaatcca aaagtgactt tatactcacc tatctcttga
    43861 gctagcggtg tttccatcat gacatgccct tcatttgtcg tatctcctga agctcgccca
    43921 ggtcgccaag caagattagg ttttccaagt tcccctgttg ttgaaaataa ggttatgatt
    43981 aattgatttt ctatacgttc atactctttt aaaccatctg cataaaacgt aaaataatga
    44041 tcattttctg ccacagagac actcttatcg aatatttcta aattaacagg tttctctacc
    44101 atagtttctg accaattcgg aggaatgaca tcaggggtat tttcaataaa tccgttttga
    44161 atttgtgcaa ttgatttttc agatttatct aaaacattaa ttgctaagcg catacgatgc
    44221 gagtaaacag tattatgaat tttgagtgtg ccttcaacga tttgactttc tcccagactg
    44281 aacgatattt cataagatat ttcttccatt tcgggttttt cagataaacg atcttttaaa
    44341 tctttaggaa gttgagcttt tccgtaaaca actaatgttt cttgtaaaga atctttatat
    44401 actttagctg tttctaattt tagagatagt tctgtatctc cttctaatgg tgaataatca
    44461 tacgtatcgc catcatttgc tgaatcgatc aaatgaacga aatcaagata ttgtctatct
    44521 ccaactatca aattcaattg cccatcttta aaagaaagtt tatatttatt gttacttatc
    44581 tcagttttgt ctatctcgat tgctgtttct aatttttcat tatttttctc aaattcaaca
    44641 acagtgtatc ctagtgctgg cacatttaca tttattttta tgtcaagttc ataataagct
    44701 ggttcatcaa aatattcgaa acctgtagca atcatccgat aagcattttt ccgttctgga
    44761 aaataaactt ctttttcaat gatattatag ggactacctt tgaagtgtat tttctttgaa
    44821 tcagctacta catgcacgat tttcgatcca ctataattct cagtcgttgt gttgaaaaca
    44881 aggacttcat tcgattttag ctttaatgca tcagccatcc gttttacaat aaggttttcg
    44941 attccgtcag ctatctcatt agcttctttg attcgatgga aaatatcttc cgcaacatta
    45001 tctgaaacac agccaccaat actatcatga gcctggtttt gcaaaatctt tttccataac
    45061 cgacgaagta ggcctttact gatatggata ccatttcttt ctgcaatcag catgagcggc
    45121 tcaactcgtt ttagaatttt ttgttccaat ttaaaatttt ctattttcat ttttgttctt
    45181 actgagccaa tagaacgatg aacacgagag taaaccggct gacgaagttc acctttatat
    45241 tcaggtaatt cttttttgtt tctgataact tctacataag aaggataatc tgaaataata
    45301 ttgtggaatt tactgtattt attcaacttc tctacgactt gatcaaaatt taaaatcatt
    45361 gacttttgat ctacacccga aggtactaaa atatctttgt tttttccttg catatgaagc
    45421 atttcaactg cttcatccaa cttttctttt gcaaattcag gaatatcttt aattgcgtct
    45481 aaggtcatca ttccagtcat ataaccaaat ggaaaattca tggcataaac aaatttatct
    45541 cccaatcctt tccagataaa ataaggagat ggaaccatcg tttcaaagtt taatcctcgc
    45601 caacacatga aattatcgat ttctacttga tgcaaaagtg taggcaaatt cgcattgaac
    45661 ccaaacgtgt caggcaaata ccctaccatc attggatctc catatttaag gatcgtatca
    45721 ctgattccaa tcattaagtt tcttaaaatt gattcagcat ccactaataa ggcatctgtt
    45781 tgtgcatacc acggtcccac gaatagttgt cctgttttca tcagtcgttg gataacctta
    45841 agcttttgcg gattgatctc caaatactca tcgacgattg aagtttgtcc atctaaacaa
    45901 aagtttactt tagggttcct ttccaattca tctagaactt cagtaaagac ctgatcagac
    45961 aatacgatgg catcttcatt tgtaaaatac cattctctgt cccagtgcgt gtgttgaatt
    46021 atatgtgcat ttaccatatc agattctcct actttccttc ttctactgaa tttacttgtt
    46081 gttctttttt atacttgatg gattttagtg cattgaccat taacgctgta accactcccc
    46141 caataacaat tgatactaaa tataaaggta tattcgtaac aaaaggaatt gccataaagg
    46201 ttgaaagcat tgtttcttga gtaacaccaa aaaatccatt tagaccacat gctactgcag
    46261 tgcccaccac gatgcttggt attactcgta aaggatcttt cacggcaaac gggatcgtcc
    46321 cttcagtaat ccccacaagg cccataatca tagctgtctt tgcattatct cgttccgatt
    46381 catcataaag ttttttgttt aaaattgttg ctaaaaacat tccaaatggc ggaatagcaa
    46441 ttcctaataa catggctgta ctaggtccaa agcttccgct tgaaaaagaa gcaaccccaa
    46501 aagcatatgc gattttattg ataggtcctc ccatatcaaa cgccatcatt cctcctaaaa
    46561 tagccgacat caaagcagca tttgctcctg acatgccttc tagccaatta gtcagtcctg
    46621 ttgtcattgc ttgaataggc gtagctagta cataccataa tatgattcca acaactaatg
    46681 tagtaacaaa aggaatcagc ataattgatt ttagactaat aacttgcatc ggtactttta
    46741 cttttttcaa tgcattagct aaatagccac aagctaatcc agcaaccatt cctccaataa
    46801 atcccatacc aagattttga cacatcatcc cagtgataaa agctggtgct agaccgggac
    46861 ggtcagcaat tgaatatgcg atataggctg aaatcatcgg tacgataaac tgtaatcccg
    46921 tctgaccaat cattcgcagg actccccaga atgtatccgt ctggtcccaa acacctgtgc
    46981 caccaaaaat gactgcaata gaagttagta accctgctgc tacgacaagc gggatcatat
    47041 aggaaacccc actttggaaa tgatttagaa tttctcttcc ttgtttcttg aattccatta
    47101 gtaactcccc cacgatatat tttttatttg ttaattaatg caattgcttc atcaatcgtc
    47161 tcttcaccat cttttactac ctctgaggtc cctactgtta aaatcggttt accgtcaaag
    47221 cgacctcgtt ctgaaacatt tgtatctact gcaaatatga caacatctgc ctcggaaaca
    47281 tccgaaattg aaagtttgtt ttctatcccc atagccccct gtgtttctac tttaatatct
    47341 aaccctttct tcttcgcaaa cttttctaaa ttagcttttg ccatataagt atgggcaatc
    47401 cctgcgatac atgctgttac tgctactatt tttgtcatat gaaattcctc cttttaaaat
    47461 gatagagctt ttaccaattc ttttttattt ctcgaatctt ttattctttt aacaaactcg
    47521 tcatccatca atcttccaga taattctgct aaaatacgta aatgattagt acctttatca
    47581 gtgtctttta ttgctaacaa aaagacgaga tcaactggtt gatcatctaa tgcgccccat
    47641 tcaacagacc gctttaactt ggctacagcc actgttgatt caattactgc taggcttttg
    47701 ccatgaggaa tggcgatatt tttcccaatt cctgtggtca tatggttttc tctgtcaaat
    47761 acgtcttttt gaaattgttt gtggtcagac acaaacccat tagtctctaa caatttcgat
    47821 aatttttcga ctacactttt tttatcagat gcatttaaat ctatcactat attatttata
    47881 ttgatttttt cactgatcat ttataagcct tctttcaata tataaactaa tttttcaaac
    47941 gacgaactcg tttttaaatt tgctaataac tcatgattcg aagcgatttc attgaatgtt
    48001 tcataaattt tattcaattc aggttttact ttttcattaa gtgcaaaaaa gaaaacagaa
    48061 tgaacaagtg tcccatccca atcaattcct tctggattaa cgtagacaca gattactggc
    48121 ttttttataa aatctacttc gatatgcggc atagcaaaat cattcatcgc tgttgaagat
    48181 agtttttctc ttttgattgc tccctctagt atcccttctt tcgcgtaatt ttcttttacc
    48241 aattttttgg taattatatt taaaacgttc acttgattat cctttatttg gtgggtaaaa
    48301 aagcaaaatc gtttatctaa taaatttaga aaagcatctt ctgtttgttc atttaactgt
    48361 agatactgtt ttacttttct gacatctgcc cctaacatca aaggactaac aacaataaca
    48421 ggaaaatttg tattttcaat cggaatcgtg cttataacaa gttcatcagt gacttttcta
    48481 tcttgtagtt cattgatgct tagtacatct tctatctcaa ccatatctgc aaaaccgtta
    48541 tttattcttt gctctaataa ttttgatgtt ccatatccgc tactacaaac taatatcact
    48601 ttcgttttgt caggtttgta acgatccaaa agtgattgaa tatggagtgt tacgtaagcg
    48661 atttcatctt cattaaaatg tatacagaat ctctcttcga tttcctctaa taaatctaca
    48721 cttatcataa atgacctttt aaaagaatat ttgattttgt ccgtataagg gttatagata
    48781 tttactccca atttgagtct tttaactgct gcattgagat gtaatactag attattgatc
    48841 aattcttcat caggataaaa ttcatttaat ttaattctta ctaactcttg tagctcaaca
    48901 cccatatcta aataggaaag cttcgtttga tctaagtcgt gattcatagc atttttttca
    48961 atcgaagtaa tgtgaatgtc aacataagct tgttcatcta ttggcacaat aattgaaaat
    49021 gctttttcaa tcattttgat taaaataagt gtattctttg agatttcttc ttcagaatta
    49081 ttactacgtg ataagaaatt gcttgtttca atcctttcta tcgcaatagc aaggtggata
    49141 gctaaagttt gaaattcata atctgtaaat ttcaattgtg tcttattcat gtatcgatta
    49201 agcaattcgg ctactttttt caatatgtcc ttatttaaga aattattcgc ttcaccaatg
    49261 aaattgatat tcaagtctaa gagatctgtt ttactgttag atgctgtaaa gccaccccaa
    49321 taatagttca caacttctga catcaacctt cttttacttt tttcatcagc tttgatgcgc
    49381 acgccatttc gatctgaaac taaggacatc ccatgcttgt ttagtctttt tctaacatct
    49441 ttcattgtgc tttctaccgt cgacctgctc acgtataatt cgtcagcaag attttgaatc
    49501 tttagagaat cattagtatt caataattta gcaaacacat aagttagacg gtcctccgga
    49561 gagtctgcga tattgatttt atgactggat aataaatttc gaagtgtttc tttttttcca
    49621 ataagcgaaa cgcctttttt aggttgaacc aacatttgta catctggcat tttttggatt
    49681 atttctttca aaattaaaat atctttgcta attgttttcg tagatacata taattgttct
    49741 gataattctt tgtaagttgt gtgctcattt tcaatcaaaa agtatagtat tttcatttgc
    49801 cgttcgctaa attcgatgcc cattatcttc acctcctgaa tacagcttaa gtgtacaatt
    49861 attatctcta ttttcataca gaagaaagtt cttgattaat gtagaagttt ctaatattct
    49921 tgattatttc aaatcaataa aaaagcctgc aatatttata tcacaggctt ttttctctat
    49981 taaatatata taagataata catttttcta ctagttctca ctaatcttgt ataattaaac
    50041 cctccgcaac ttgacggaga tggagaagat gcccccgtct aatctagcgg tcatctctcc
    50101 tcccattcct tccaccaatt tttttgacag gtaaagcccg attccgctag attccgtatt
    50161 tgtgcggctg gtattttcgg tataaaaacg ctggaagact ttttcaacag aggtttgcgt
    50221 tcctggtttg ataggattgg ccactgttaa tcgaatgaat ggatcctctt ttgaaaatgt
    50281 cacctttgct tgtccatctg cataacgcaa taaattctgt atgatatttt gcagcactcg
    50341 cagcaacatg agcggatctg ctaatacagt tgtatttatc gagtgttcag cgaacgctac
    50401 ctcgatattt ttctcagaaa attgttcata aaaagagaaa aatacttctt ccacctcttt
    50461 agaaaaagaa agcgtatgga gatccatttc tttttgcttc gtctctattc gagcaagatc
    50521 ataaaactga tccgtcatac tgagtaatct tgcaacagat tgtttgatga tcgttaaata
    50581 cttctcccgt ttctccgtat cctctacctg ttccagtaat tgtacataac cgttaatagc
    50641 agtcaaagga gttctgagat catgagaaag gccgacgatc gatagtctga tgttttcttc
    50701 catttcttga aatgcttgtc ctttttgttc attttcatcc aaccatgtat tcagtgcatc
    50761 tactaggtcc attagattct tttctcgaaa ttcgactgaa agacgaccac catattcttg
    50821 tcgttcagaa agagtggcga ttttcttagt taatctcttg atttcccttt gcaggaagaa
    50881 aataaaactt cccataaaga taacaacgag aagcaacagc catttaatca aatctaaacc
    50941 ccactcccca caccgtatga atatacacag catgtgcctt tgctagcttc cctcgaagat
    51001 tggaaatatg catattcact gttttctcac tatcaaaata tggttcttgc caaactgttt
    51061 cataaagatt tgctcgcgaa aatacttttt tcggatgttc taaaaaaagc ttcagcagat
    51121 cgaattctct tcctgtcaaa tggacagcca caccattcac atttacatcc ctcgactctt
    51181 tgtccagtaa gatttccttg taatagatgc gttgcgacag atcttttact tggacagaag
    51241 tctgatgacg aagttggatg ccgattcgta acaaaagttc ttcgatatca aatggtttcg
    51301 tcacatagtc atccgctcct gctgccaaca aggaaacttt atctctttga tcgctttttg
    51361 ctgaaagaac gatgatcgga acattcgatg tctgtcggat ttctgctaac aatgcttcac
    51421 ctgaaagccc tggaagcatg agatctagaa ggatcaaatc gacaggatat gtgcttaaca
    51481 ataattttcc ttccgtacct gaataagcat caagaagttt gtatctctgt tcaagaacct
    51541 cctccagtaa ggaatgaaca gcttcatcgt cttcgataat caaaatagtt tccagcagga
    51601 ccacctactt aatgtcagat ttttggaaaa taaacattcc tagagctgat gataccacga
    51661 ttgttactag actgaccaga atcattttcg gtgcttgcga ataagttgct tgttgcgcaa
    51721 agtccgtata catgagccac gtttccgtaa accactccgt tagaaattcc aagcctttga
    51781 tttttgcgaa aacaccggga atgaaggccg ttcccatgat ataaagaaaa gaaagaagca
    51841 aactgacact ggtacttcta gtgatgaatg tcaaagccac tggaacagat aatgtcccta
    51901 atgtcagcag aagcaataca ggaaatactt ttagaacata atttccatag tttttgattc
    51961 cctcaacttc accaaatccg aaagcaagtg tgtaagcaag catggctagt cccgtgatca
    52021 aaaccacgcc aagtgttgtg atcaaaagcg taacgagaag tttcgacaca taataagtca
    52081 ttcgattccg tcctaaagat aacgtattgc gaatcgtacc gttttgaaat tcttccccaa
    52141 tataaaagca cgcaaatgct gcgaaagcca gaagaatcca tgcagccatt ttttctacca
    52201 ttaaaatccc tgttgcaccg ttgatgactg tatccgttgc ttggtttaat tcattgccgt
    52261 attttgtatg catataccat tcgttccaag aataaaaaag cggaagtacg accatcagtc
    52321 ctagtgagac ccacatttta cggctcttcc atacttttat tttttcagca gttaatagat
    52381 taaacatgtg gattgcctcc agttaattgt aagaaataat cttccagttt ttgcccaact
    52441 aggtgaatac catccacagg aacttttgct tctaccaatg cttgattgat ggctgctact
    52501 tggtcaattc cttcaaaaag ctggataaca cggctagatt gtacaaaata atccctataa
    52561 ccaagagagt cgagaaccgt tactgcttgt tccgtcgccg aagtgtcaat ttttataaat
    52621 tgacgagatt cttgtgccaa ttcttctttc gataattctt tgatcaagtg tccttcatgc
    52681 aaaataccat agtgagtggc gatctgtgac aattcatcta gcaagtggct agaaagcaag
    52741 acggtgatgc cccgttcttt taccaaacgt tggatgattt ctcgcatttc tgccatccct
    52801 gctggatcca tcccattcgt cggttcatcc aaaatcaaaa attctggatt cgtgatcagt
    52861 gcatttgcaa tagctaaacg ctggcgcata cctaaagaga aattctttgc tttctttttc
    52921 cctgtatttt ccaatcgcat cattttcagc aggtcttcga tttctctatc cgacacaccg
    52981 ccgttcgctg cttggatacg taggttatct cttgcagtta attctggata aagagcaggg
    53041 gtttcaatcg attgacccat acgtcgtctt gctgcctgca aatccgtcgt tccaaataat
    53101 tcgatcgaac cttcatcaat cgagatcaaa cccataatag ttctcataaa ggtagactta
    53161 cccgctccat tttccccaat caatccatag atcattccac gtttgatttc gattgatact
    53221 ttatctaatg ctttggcagc accgtatttt ttggttatac cagttgcttt caagaccgtt
    53281 tcatttccaa aatccatact tatccttctt tcttattgat gagtgaagta tagttgacgg
    53341 gtataaagaa agcagccaga aatgtataaa gaaagtgtaa attttccaat tgaatgtcaa
    53401 aaaaaacaat cggacaatac gtgtctttcc tattatcagg cagtttgtta tacgcttgta
    53461 tcagtttcct gattctttct tttcgaccaa agtcgtttct ctgtattcct cttcactgaa
    53521 aatttttgct tctttcagat caagcttgag taattttacc agaccggtta taaggataac
    53581 tatagcaaga ctccctccca gaaaaattag actgtctcgc agacgtggtt gcgttgtctt
    53641 ccaatcaaag agcgcaccaa acagtgctaa tccaagtgga ctggccacca tggtaatcgt
    53701 tgttatcgtt gacataagtc gtccttgtat atgcgttgga acttctcgct gaagccagac
    53761 catataaaaa gcaaaagaaa acattaatcc ttaaaaacgg acttatgaaa aataatacat
    53821 gccgtttggg atttacaatt agctaccaaa taagaggtta tgacaaaaga cttttcacaa
    53881 tcccttcttc cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt
    53941 tatcaaacat gagaagcaat ttttgtaaat tccttcttat ttcttgaagc tgaccacttc
    54001 tgtcacaacc tcagaagcgg ataaataata ttgtttgatg atactttatc tttttcccgt
    54061 ttgtttatag atcgaatgat attatagacc aagaatccta cctattaaca tagctaaaaa
    54121 cacaaaaaag gcactttcca aattgtggaa agtgccagaa aagttaggat taaggtaact
    54181 gcgctcagca ttgcttcgcc cagtacgatt ctcgtaactc gtgaactcga acgataatcg
    54241 caattaacgt tttgagaact gtgaagcttt acgggctttc ttaagacctg gttttttctt
    54301 cgtatcattg aataagtttg aaaaaccttg atttataaag gttttcccgt aaatatattt
    54361 tattatattt gattaaattt gaaatgtttg tgttctaaaa tgtgttctaa tttaaaggca
    54421 gtatatttcg tattttagat taaattttta ggctcattca ttctcaaatc cattctattc
    54481 agtcaataaa aattttaaaa agactttata aacaaagatg ttcaagcgag catcttattt
    54541 ttttgtgctt aatttgaaag gagaacattt catgtataaa aaacaaaaaa agaaattatg
    54601 tgtattaact attttgagtt tggctgttca agcaattgtt ggagcaattg ctcctacgat
    54661 tacttttgct gatgaaatta cccatccaca aacggtaacc gtagaactag atttagcaca
    54721 tcaatatgct gttgaaggaa cttttagtga tggtcgtcca atgtcagaag tcactgttcc
    54781 gcattatgca gtgtacaacg gctttaaaca agatattttc tgtattgaac caggagtccc
    54841 gatttacaat gagttcactc ctggctatga gaaaaacccg ttaccagata tgtccgataa
    54901 agccaaatta gtttccgttt tatggaaaaa agtaggtaca gatgcagata ctcatattgt
    54961 ggcacagaag atgatctggc aagaagttaa tgggtatacc cttcactcaa taaaacgatc
    55021 agatggtagt gccgtaaata tcgcagccat tgaggcaaaa ataaatcaag ccattgacga
    55081 ttatcagaag aaaccaacct tccataatag tacggctaaa acagtattag ggcaatcgac
    55141 tactgtgacg gatacgaata atctgaattt gtcagagttc gatgaggttg tggaaaatgc
    55201 cgcaaacatt gattatcgtg tgaatggtaa tcagttgata atcaccccaa atgccaattc
    55261 aaaagaaagt ggtgtgctga cgcttaagaa atctgctggt accggaacac ctgtcgccta
    55321 taaaaaggct ggccaacaga ctttaatggc cggggcgatt gataaaccta atacctacac
    55381 aatcaaaatt gatgtggaaa cggagggttc tttaaagatc aaaaaagtcg ataaagaatc
    55441 gggtgatgtt gtaccaggaa cggttttcca tttagacttt ggaaaaactt tacctgcaaa
    55501 agacgtgact actgacaaag aaggcattgc tacattggat gagattcctc atggtacaaa
    55561 agtaaccatt accgaaaaat cagtaccggc gccttatacg attgatacca cccccatgac
    55621 taccaccatt aaagcaggcg aaaccattta cgtgacttca aaaaacgcac gggaaaaagg
    55681 gcaaatcatt ctggataaaa gcggagttga aacagggagt gatctttgga atgacaatta
    55741 ttccttagct ggtaatacgt ttgccattcg caaagacagt ccaaccggtg aaatcgttca
    55801 agaaatgacc acggatgaaa acggtcatgc ggaaacacca aaagagattg ctaatgcgtt
    55861 ggaattggga acctattatg tgaccgaaac taaagctagt catgggttcg tgaatacgtt
    55921 caaacctgta aaagtcgaat tgaagtatgc caatcaaacc gtagcccctg ttaccagtaa
    55981 cgtaaaaggg caaaatcaag aagtcactgg cgaaaccact ttgacgaaag aagacaaaga
    56041 tactggggat aaagcacaag gtaaggcagt ctttgagggt gctgaataca ccttgtttac
    56101 tgctaaggat ggaaaagctg ttaaatggag tgaggctttt aaaccagagt tagcgaaggg
    56161 aacgaaaggt tctgatgaaa cggtcacttt gactttagac gaaaagaacc aagttgccgt
    56221 taaacattta gctatcaacg agtattattg gcaagaaaca aaagcgcccg aaggatatag
    56281 ccttgatgaa acaaagtatc ctgtatccat caaaaaggtg gatgatagcg aaaaaaatgc
    56341 cgtgattacc cgaaatgtta cggcaaaaga acaagtgatt cgctttggct ttgatttctt
    56401 taaatttgct ggatcagctg cgggcactac cgaaactggc ttcaatgatc tgacctttaa
    56461 agtgtcgcca ttggaaggga ccaatgaaat cacaggtgcc gaagatgaag cgaccacagc
    56521 ttataacgag caattagggt ttgatggtta tggcaagttt gaaaatcttc cttatgggga
    56581 ttatttactt gaagaagtag aagccccaga aggttttcaa aagattacgc cattagaaat
    56641 ccgttctact tttaaggaaa acaaagaaga ctttgtgaag agtgagtatg tctttaccat
    56701 taccgaacaa gaccaaaaac aaccaattaa aacggtgacc gttccttacg agaagctgac
    56761 gaacaaagca ttttctgtta gtttgaaccg tttaatgctc tatgatttgc ctgaggaaga
    56821 agatagttta acttctcttg cgacttggaa agacggaaac aaagaattaa cttctctgga
    56881 ttctaccgag ctagttgata aattgagtta caatctacat gaaatcaaag aagactggta
    56941 tgtggtagcg caagccattg atgtggatgc gacaaaagct gcccaagaaa aagatgaaaa
    57001 agccaaacca gtggtgattg cagaaacaag cgcaacgttg gcgaacaaag agaaaacagg
    57061 aacttggaaa attcagcata aattaaccgc cgaacaagtt ttaaataaaa ctattgtctt
    57121 attcaactat gtgtatgaaa ataaggaagc ttttgaagca ggcgataagc cagtagcgaa
    57181 ggatgttagc ttgaataacc aagcccaaac tgtcagttgt acagtggaac accatgtttc
    57241 tatccaaaca aaggcccacc tagaaaacgg ttctcagacc tttactcatg gtgatgtggt
    57301 ggatatgttt gatgatgtgt cgattactca tgatgtactg gatggctcaa aagaagcttt
    57361 cgaaacaatt ctgtatgctt tattaccgga tggtacaaac aaagaaattt ggaaatctgg
    57421 caagattgat tatgaagtaa atgataaaga attcaccaaa actgtgcttg cgaaaaaagt
    57481 agataccgga aaatatccag aaggaaccaa gtttactttt gcggaaatca attacgataa
    57541 agatgggaat atcaatggaa aacacaatga agatttgaaa gaaaaatctc aaaccttaac
    57601 accaaaagaa gttccaacca tactaagtac gccgaaacaa ccggaaacac caactgttcc
    57661 aagtggctct caagaatcta gtcccacagt gaagaccttc cctcaaaccg gggagaaaaa
    57721 ttccaatgtt ttactgttca ttggttttac tttgattttt gcgacggcgg gctattattt
    57781 ctggaatcgc cggaactaag gtgatgagat gatacaagta aatcctaaga accaacgtgc
    57841 gcgaccgcct cctaaaaaag tagtcgcgct atttttgtat ccaaaactaa caatgaaaag
    57901 aggaaaagaa gatggaattg aaatttgtcg tgccagatat ggctgaaacc tttggaaagt
    57961 taagttatgc cggtgaagga gaaattataa cagaaggata tggacggaat actacggtga
    58021 ttggtcgcag ttatcatttg tattccagta agcaacgggc cgatgatatt gaggtggtag
    58081 tagctgcgga agccggtgaa aaggattttg atcaagacca accgctaaaa gccgtgaatc
    58141 cccatttggt tgccaaaggc tatgagattg agaatcgtgg gtttactgat tacgtgttgt
    58201 atgttgatga tttagtgaaa gcataggagg aaaagaagat gagattaaca gaaggtattg
    58261 tcgtagattc aggtttaaca tttgggaagt tgcgtttttc cgcattacgc cgagaagtac
    58321 ggaagcaaaa tgaggatgga acaattggta atgaggtaaa agaacgaacc tataatttaa
    58381 aatcctctgc acaagggcga atgattcaag ttagtatccc agctaatgta cccttacgcg
    58441 agtttgctta tgatgcagag gtagagttgg tcaatccaat cgtggatacc gttgccaatt
    58501 atgtttttcg agaaggaact accgttaatt ggttcattaa ggcggatgat ttagtcttga
    58561 agcggcagcc gaatcaagga aatcctacga accaaaatga aagaaagaaa taggtgaaga
    58621 ttatggaaat taatgttgaa catattttag attgtctcga tcaatatgga aaaggcgagc
    58681 tgactgagga acaactcgtt aaagcattaa cctctgatga aaaaatgttt ctgattatac
    58741 atcaaggttt acttggagaa aatagcgaca gtgaagaaga ctctgatgta ctagactggt
    58801 taggagaaga agagtctttc tttatagttg tcgaagtaaa tgaaaactta tgtcggcagg
    58861 cagaatccat tttggaagaa attggtgtgg agatgccgga tgctattgaa ggctttttaa
    58921 atcaattggt tgaaacgaaa caactcccct cctcgtagca gtaaatgact agcataactt
    58981 tttgaaattg cctaatgaaa tcctattggg ctttttcgat tggaggtgga aaccatcaaa
    59041 atgtacaaag gacatcgcat acgcgcagga gatcagcact tggtttatca ctttgttctt
    59101 ggctggctac tcgctctgtt catcggttgg atgagcgtct tttattttca agagtttaga
    59161 caatttgata tttctaagct atcactttct actattgaaa tcgtttggtc tataaaagat
    59221 ttagtatgtc tgcttggaag tctcgctttt tcaggagcta tgatattgct ttatattcac
    59281 ttttttcttg atcattggcg aagcttgtgg catcgacaaa agctagctcg gatgattcta
    59341 gagaaccatt ggtatgaagt gaaacaaact caaagtgaag gctttttcaa agatttgaat
    59401 agtagccgaa ccagagagac tatcagttac ttccccaaaa tctattatcg aatgaaagat
    59461 ggattactgt ctattcgcgt tcaaatttca cttgggaaat accaagatca gcttttaaaa
    59521 ttggaaaaga agttagaaag cggactgtat tgtgagttag tagaaaagga actcaaagac
    59581 tcctatgtgg agtatacttt actgtatgat atgatcgcca atcgaattgg gatagacgaa
    59641 gtagtggcag aaaatgggac tctgcgattg atgaaaaatc aagtatgggc ttatgattct
    59701 ttaccccata tgttaatcgc tggtggcacg ggtggaggga agacctattt cctcctcacc
    59761 attatcgaag cactattgaa gtcagatgca gaattgttta tcctcgatcc caaaaatgca
    59821 gatttagctg atttaggtac ggtgatgcct catgtttatt cacaaaagga agaaatttct
    59881 gcttgtgtgg aagatttcta tgaacgtatg atggctcgta gcaaggcaat gaaagaaatg
    59941 tctaactaca aaacaggaga gaattatgcc tatcttggac ttccacccaa ctttttaatc
    60001 tttgatgaat acgtggctta tatggagatg ttaacgacga aagaaagtgc ggtgattttg
    60061 aataagctca aacaaatcgt gatgttgggt cgtcaatctg gtttctttct gattttggct
    60121 tgccaaaggc cggatgcgaa atatttagga gacggaatcc gcgatcaatt caactttcgc
    60181 gtggctttgg gtcgtatgag tgaactcgga tattccatga tgtttgggga ggtcgaaaaa
    60241 aacttcttta tgaagcacat caaaggtcga ggttatgtgg atacaggtgg cagtgtgatt
    60301 agtgagtttt atacgccact tgtaccaaaa gggtatgatt tcttagattc aattagtaaa
    60361 ttagaaaaga tgaatgattg agtgatcttc aatactgact ttatacttca agtaacgtat
    60421 aatactttta tatagtagga agaaagggga taattaatat ggaaagtaat gattcttatt
    60481 tagagtatgc aaaagcaaat aaggataagt ttatttctta tattatcaaa gggaaatccc
    60541 ccgaagttgg acgagacgca gtttttatgg cagggagtcc tggggctgga aaatcagagg
    60601 ttgccattgg attagctgag aattatgata accatgtgat tattgatgcg gattactttc
    60661 gcacccagtt tcctgattat aatggaaaaa actctagtct ctttcaaaaa gcatcttctt
    60721 ggttggtaga gcaggcattt agatatgtct tagatcatgg ctattctttt attctggatg
    60781 caacttttgc aatattaagt gctgaaaaaa atatctcgcg ggcgttgaaa aaacattttc
    60841 gagtgacgat tttctatgtt tatcaagacc ctaaaatcgc ttggaaattt acacaagaac
    60901 gtgaaattgc agagggacgt catgtgccga aaaaggcatt tattaacgct tttttcaaat
    60961 caagagagaa cattgcaaaa gtaaagagta aacatccaga tgtattgctt catatcataa
    61021 taaaagatta tacgaataat atttccgaag ttcattatgc tacggataat ctagaattat
    61081 ttttaccact agggtatagt aggaaagatt tggaggttca attacatgac tgagaaagaa
    61141 caagtaacga aaattgtaaa gaaatacaat aagagcattg cggatttatc ggaaaatgct
    61201 actgcaaaag agttcaaaac ggtgattaaa tatgtagccg atcaggccaa tgaaaaacaa
    61261 cggaaattag ttggattaaa taaaaaatag taggacaaat taataatgta tgagccaaga
    61321 gcaaaatgtt cttggctttt ttctttgcaa tgaatgaggt aatgtatgag atttttagat
    61381 ttattcgccg gtattggtgg ctttcgttta ggtatggaac aggcgagcca tcattgtatc
    61441 ggtttttgtg aaattgataa atttgctaga cgtagttata aagcaattca tgatacaagt
    61501 aaggaggtgg aaatgcatga catcacaagt gtatcagatg agtttattca atccctcgga
    61561 ccagtggaca tcctttgcgg cggatttccg tgccaagctt tttcaattgc gggaaagcgg
    61621 caaagatttt ccgatactcg aggtacttta ttctttgaaa tcgctcggtt caccactctt
    61681 ctccgaccta agcttttatt ccttgagaac gtccgaggat tgctcaatca cgaaggaggg
    61741 gctacgttcg agacgatcct ccgaacgctg gatggattgg ggtatgacgt ggagtggtag
    61801 gtgcttaact caaaggccta cattccccaa agccgggagc ggatattcct tatcggacat
    61861 tctcgagacg catgtaccga acaagtattt cctatcattg gaccgtcttc aacatctgat
    61921 caaaacatca gaaatttggt aaatatcaat ccttccaatc gaggaatggg tggacaagtc
    61981 tatggttcag atggcgtagc gccaacctta acaggtgatg aaggaatcaa aatcgcactg
    62041 ccagcaaggg atggaatctc tgtagcagga ctattaccgg gaaagtttga acaaggtaat
    62101 cgagtttatg aagttactgg aaccgccccc accttgtcaa cgaaacaagg tggtacaaaa
    62161 atcatgattc gaaaaaatga aacagaatat caagaggtga agccagaaga cagtgtgaat
    62221 ctggcttttc ctaattccac aagtcgccga ggtcgtctgg gaaaacagag tgttcatacc
    62281 ttactaacag gagaccaaca agcgatcgtc acggatcagt atcaaattcg gaagctaaca
    62341 cctagagagt gttggcgact tcagggcttt cccgattggg cttttgatcg agattctcaa
    62401 gtcaattcag atagtcagtt gtataagcaa gccggaaact ctgtaacagt tccggtcatt
    62461 tttgatattg ccagaagatt aaaagagttg gatgtacatg gtttatgatg agttgattgg
    62521 agaaatttat tgggtgattg gaaagattca gtcagaccca gaattagaag aacaattgcg
    62581 acggttaaaa tttgatattc gcaagaatgg tgtcaaagtc gctggcgatc catatttaat
    62641 gaatgaggag acggatgctc gaattgaaat aaatcaagta attgcagaat ttgagcggat
    62701 tgcggaccaa gcaaaagaac cagatattcg tcaatacttg ttagaaatga aagcagagtt
    62761 ggaagtaaat ggtattacag atgagtaacc taaaccaaca caactgactg ttaggcgctg
    62821 accgcggagg attcgcgaag cagaaatcct ccgtggtgct gtgcctgttc atggacggcg
    62881 ttagccgacc atgatgtctc acccccgtat ctaacagggg ggtacaaaaa cacaaaaaat
    62941 aagtaagcaa ccattaagaa tgcttgttgt atcaaagata gtaagtaatt tgtataggtg
    63001 ttaactcatt tgaaaaagtt aacaccttga atttggaggt gagaacttgg cacaacggaa
    63061 tttagattat cggctattaa aagaccgacg caacgaatac ggcgtttcac aaaataaatt
    63121 ggctacggct tgtggactta gtcggccgta cttaaatcag atagaaaatg gtggcgtaac
    63181 cgcatctata aaaaccatga ggaaaatttt tgaccaactg gaaagcttta atcccgattt
    63241 gcctttgacg ttactgtttg actatgtaag gattcgcttt cccacaacgg atgcacgaaa
    63301 aattattcaa gaaattctcc atttgaaatt tgagtatatg ctccatgaag attatgcctt
    63361 ttactcctat caagaacagt atgtcatggg tgatattgtg gtgatgttgt ctcatgaaga
    63421 agataaaggt gtgcttttag aattaaaggg gcgaggttgc cgacaatttg aaactttttt
    63481 actcgctcaa aagcgtagct ggtacgactt tttcgaggat tgtctaaagg ccggtggcgt
    63541 gatgaaacgc ttggacttgg caatcaatga tcgagtgggg ctattggata tacctgagct
    63601 aacgaaaaaa tgtcagaagg aagaatgtat ctccttgttt cgtaccttta aaagttatcg
    63661 ttccggggaa cttttaaagg ctgatgaaaa ggacggcatg ggcaatacct tatatatcgg
    63721 cagtctcaag agtgaggttt atttctgttt gtatgaaaaa gactatgaac aatatatcaa
    63781 gttagggatt ccattagacc aaactgagac gaaaaatcgt tttgagattc gactgaaaaa
    63841 tgaccgtgct tttcatgcga ttcaagattt gttaaaaggt cgtagcattg agagcaccac
    63901 gttttccatt atcaatcgtt acttgcgatt tgcggataaa gtagaaggta aaaggcgaac
    63961 caactggccc ttgaatgaac aatgggggcg ttttattgga cggaatcgga aagagattca
    64021 attaacttcc gagccaaaac cgtataccat tgaacgaacc ttgaattggc taggccgaca
    64081 agtcgcccct acgtggaaaa tggctaagga gttggatcga ttgaatcgga caacctatat
    64141 ccaggatatg gtacagaatg cacgcttatc agaccgacat aaaaagattt tggagcaaca
    64201 aagtatggca attgaaaatt tgattatatg aaaagaggaa tcgtcatggg aattgtaaaa
    64261 gatatttttt tggtatttgg tggcagtatg cttggtgtta ctgtgatgtg cttgatgaat
    64321 gcaagtgcag cagcggatcg agcaatggaa aagaaaggaa agtgacaaga tgaatttcgg
    64381 acaaaattt
//
LOCUS       DAMYXM010000008        64150 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.008, whole genome shotgun sequence.
ACCESSION   DAMYXM010000008 DAMYXM010000000
VERSION     DAMYXM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.008"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="PNS21_000614"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="PNS21_000614"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDI5158346.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="PNS21_000615"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="PNS21_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDI5158347.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="PNS21_000616"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="PNS21_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDI5158348.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="PNS21_000617"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="PNS21_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDI5158349.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="PNS21_000618"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="PNS21_000618"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDI5158350.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="PNS21_000619"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="PNS21_000619"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDI5158351.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="PNS21_000620"
     CDS             complement(8053..8523)
                     /locus_tag="PNS21_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDI5158352.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="PNS21_000621"
     CDS             complement(8569..8994)
                     /locus_tag="PNS21_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDI5158353.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="PNS21_000622"
     CDS             complement(9061..9330)
                     /locus_tag="PNS21_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDI5158354.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="PNS21_000623"
     CDS             complement(9341..10936)
                     /locus_tag="PNS21_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDI5158355.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="PNS21_000624"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="PNS21_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDI5158356.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="PNS21_000625"
     CDS             complement(14489..15049)
                     /locus_tag="PNS21_000625"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDI5158357.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="PNS21_000626"
     CDS             15402..16376
                     /locus_tag="PNS21_000626"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDI5158358.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="PNS21_000627"
     CDS             16546..17946
                     /locus_tag="PNS21_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDI5158359.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVNLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="PNS21_000628"
     CDS             complement(18024..18746)
                     /locus_tag="PNS21_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI5158360.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="PNS21_000629"
     CDS             complement(18825..19331)
                     /locus_tag="PNS21_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDI5158361.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="PNS21_000630"
     CDS             19454..21253
                     /locus_tag="PNS21_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDI5158362.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="PNS21_000631"
     CDS             complement(21321..22895)
                     /locus_tag="PNS21_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDI5158363.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="PNS21_000632"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="PNS21_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDI5158364.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="PNS21_000633"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="PNS21_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDI5158365.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="PNS21_000634"
     CDS             complement(24672..25310)
                     /locus_tag="PNS21_000634"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDI5158366.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="PNS21_000635"
     CDS             complement(25311..25961)
                     /locus_tag="PNS21_000635"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDI5158367.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="PNS21_000636"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="PNS21_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDI5158368.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="PNS21_000637"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="PNS21_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDI5158369.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="PNS21_000638"
     CDS             complement(29104..29844)
                     /locus_tag="PNS21_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDI5158370.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="PNS21_000639"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="PNS21_000639"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDI5158371.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="PNS21_000640"
     CDS             complement(31215..32153)
                     /locus_tag="PNS21_000640"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDI5158372.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="PNS21_000641"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="PNS21_000641"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDI5158373.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="PNS21_000642"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="PNS21_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDI5158374.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="PNS21_000643"
     CDS             complement(35313..35618)
                     /locus_tag="PNS21_000643"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDI5158375.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="PNS21_000644"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="PNS21_000644"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDI5158376.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="PNS21_000645"
     CDS             complement(36724..37008)
                     /locus_tag="PNS21_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158377.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="PNS21_000646"
     CDS             complement(37026..37916)
                     /locus_tag="PNS21_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDI5158378.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="PNS21_000647"
     CDS             complement(37936..38154)
                     /locus_tag="PNS21_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HDI5158379.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="PNS21_000648"
     CDS             complement(38124..39824)
                     /locus_tag="PNS21_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDI5158380.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="PNS21_000649"
     CDS             40091..40864
                     /locus_tag="PNS21_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158381.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="PNS21_000650"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="PNS21_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HDI5158382.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="PNS21_000651"
     CDS             complement(41688..42659)
                     /locus_tag="PNS21_000651"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDI5158383.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="PNS21_000652"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="PNS21_000652"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDI5158384.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="PNS21_000653"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="PNS21_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDI5158385.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45614..46438)
                     /locus_tag="PNS21_000654"
     CDS             complement(45614..46438)
                     /locus_tag="PNS21_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDI5158386.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46411..47091)
                     /locus_tag="PNS21_000655"
     CDS             complement(46411..47091)
                     /locus_tag="PNS21_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDI5158387.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47114..48052)
                     /locus_tag="PNS21_000656"
     CDS             complement(47114..48052)
                     /locus_tag="PNS21_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDI5158388.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48297..49160)
                     /locus_tag="PNS21_000657"
     CDS             complement(48297..49160)
                     /locus_tag="PNS21_000657"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDI5158389.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="PNS21_000658"
     CDS             complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="PNS21_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDI5158390.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52016..52822
                     /gene="proC"
                     /locus_tag="PNS21_000659"
     CDS             52016..52822
                     /gene="proC"
                     /locus_tag="PNS21_000659"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDI5158391.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52901..54457)
                     /gene="rny"
                     /locus_tag="PNS21_000660"
     CDS             complement(52901..54457)
                     /gene="rny"
                     /locus_tag="PNS21_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDI5158392.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(54868..55917)
                     /gene="recA"
                     /locus_tag="PNS21_000661"
     CDS             complement(54868..55917)
                     /gene="recA"
                     /locus_tag="PNS21_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDI5158393.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56026..57267)
                     /locus_tag="PNS21_000662"
     CDS             complement(56026..57267)
                     /locus_tag="PNS21_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDI5158394.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="PNS21_000663"
     CDS             complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="PNS21_000663"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDI5158395.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58294..59217)
                     /locus_tag="PNS21_000664"
     CDS             complement(58294..59217)
                     /locus_tag="PNS21_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5158396.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(59956..61242)
                     /locus_tag="PNS21_000665"
     CDS             complement(59956..61242)
                     /locus_tag="PNS21_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDI5158397.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61235..62503)
                     /locus_tag="PNS21_000666"
     CDS             complement(61235..62503)
                     /locus_tag="PNS21_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDI5158398.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            62755..63216
                     /gene="mscL"
                     /locus_tag="PNS21_000667"
     CDS             62755..63216
                     /gene="mscL"
                     /locus_tag="PNS21_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDI5158399.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(63275..63721)
                     /locus_tag="PNS21_000668"
     CDS             complement(63275..63721)
                     /locus_tag="PNS21_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5158400.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(63814..64059)
                     /locus_tag="PNS21_000669"
     CDS             complement(63814..64059)
                     /locus_tag="PNS21_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDI5158401.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT        17745 a        13930 c        11479 g        20996 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaataa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtca atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    45541 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    45601 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    45661 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    45721 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    45781 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    45841 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    45901 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    45961 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46021 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46081 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46141 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46201 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46261 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46321 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46381 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46441 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    46501 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    46561 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    46621 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    46681 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    46741 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    46801 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    46861 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    46921 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    46981 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47041 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47101 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47161 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47221 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47281 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47341 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47401 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    47461 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    47521 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    47581 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    47641 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    47701 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    47761 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    47821 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    47881 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    47941 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48001 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48061 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48121 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48181 aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa taaaaaactg
    48241 aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct tatattttat
    48301 tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt atatactctc
    48361 tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc actcatcaat
    48421 gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac tggatgccgc
    48481 tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg ataatcttgc
    48541 tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg cgtatctact
    48601 tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac aacccagcac
    48661 tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa tgccgtactt
    48721 ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc cagttcttct
    48781 aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc acagtctgta
    48841 cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat cttgacaccg
    48901 gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt gttccgttca
    48961 tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt tatttcttca
    49021 aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc atgataccca
    49081 tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc tttttcaatc
    49141 aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa
    49201 ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca acagctttca
    49261 cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc tgtgagcact
    49321 tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag tagctgcatg
    49381 gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc ccaatgatcg
    49441 acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt accataatta
    49501 tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc aacattagct
    49561 aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac aataaacaga
    49621 ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg caacaaatca
    49681 tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac tttttgatct
    49741 ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg tcccacagag
    49801 aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac agcttgatgt
    49861 aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat caaacgttga
    49921 tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac aatccctgct
    49981 tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta gctttctgat
    50041 atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa ctgattcgtt
    50101 gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca tttgtttcct
    50161 ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata aaaataactg
    50221 taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc caataaacgg
    50281 tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc taaatattga
    50341 taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga gaaccacaca
    50401 tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa aaagcgtccg
    50461 ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct agcatcaaag
    50521 attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct ggataaccac
    50581 acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt acgtgtgatt
    50641 gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt tttgactttt
    50701 actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt cactgtctga
    50761 tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat tgcagacaga
    50821 ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc acttacgagc
    50881 acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt
    50941 ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag aacataataa
    51001 aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga caattgactc
    51061 attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat cgtcagcatt
    51121 cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat atctattctc
    51181 atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc aaaggatgcc
    51241 agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt tctctttaga
    51301 taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca gatccaatag
    51361 cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag aaacgaccat
    51421 acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa aaccattttt
    51481 ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac accaccaaaa
    51541 ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc ttttctttcc
    51601 atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata caacaacatc
    51661 ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg gctttgttgt
    51721 cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc gacttgattt
    51781 gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact atgttccttt
    51841 atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta acttgtatac
    51901 ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt agacctgttc
    51961 tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg ataacatgaa
    52021 agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt tattaaaaaa
    52081 aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa
    52141 atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg catcacaatc
    52201 agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg aagaactaag
    52261 agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat taatagattt
    52321 agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt
    52381 cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg aggaacagct
    52441 tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag cagaatcaga
    52501 tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca tgctgatcga
    52561 tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca ctgagattgc
    52621 tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa atccatggga
    52681 acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga
    52741 agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg agaaagatct
    52801 tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca aaaaaagaag
    52861 agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca taatcagttg
    52921 cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt tcttcttcga
    52981 ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt tcttctggct
    53041 tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct acaccggcga
    53101 agctgttcga aatattttcc agacttctta aacgacggat gtagttttct aatgattcac
    53161 ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg acagagataa
    53221 cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact actgggtttt
    53281 ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca atctcatggt
    53341 caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg atatcttcgc
    53401 caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt
    53461 tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga tctggatgaa
    53521 gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata cgttcatcca
    53581 tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt cgtccatctt
    53641 gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg aatccggaaa
    53701 gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc gtctcaagcg
    53761 ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca ttcggaaggg
    53821 taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga atcgctaatg
    53881 ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca gattctttta
    53941 ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg acatctcgtg
    54001 cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct tcaatcattt
    54061 tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt gcttcttctt
    54121 tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc agcgtttgtt
    54181 cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct ctcagctcac
    54241 tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat gcttcttttt
    54301 ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat gattgtgcac
    54361 cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca agaccgacaa
    54421 ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc atactatctt
    54481 tttcattttt atcatctata cagcttctat gataaattat tctaaactcg tcaatatgca
    54541 tcttcgcata tatgtgtaca aaaaacatgc acaactttat tctaatgttt gtaccccgta
    54601 gtgtcaactt taaataaaca aagaaacggc tttctactta ttcattaaac gatggttttt
    54661 aaaataaatg acactagttt catataggta aacctttaaa ttgagaaact tttctattaa
    54721 attattcttt gtagatgaac agatattttt attttatgta cccactcgta taattaaata
    54781 actcacgatt tctttattca ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag
    54841 accagccgtt tttgtttatc ataattttta ttcttctact aaaggcaatt cttcttgtcc
    54901 tttttcatct tctgggacag cgacttcttc accaatacca taagctgctc taactagagc
    54961 agaaacttct gccatcattt caggatgatt agccatatag attttagcgt tttcccgtcc
    55021 ttgaccaatc cgatcttctt tataagaata ccaagcaccg cttttatcaa caatgtcttt
    55081 ttctactgcc atgtctagaa gttctccttc ttgtgagatc cctaaaccat acatgacatc
    55141 tacttcagcg attttgaaag gaggtgctac tttgtttttt acgactttga tctttgtacg
    55201 gttaccaaca atatctgtac cttgtttcaa ttgttctgca cgacgtactt ccaaacggat
    55261 cgttgcgtag aatttcaatg cgcgtcctcc aggagtgatt tctggattcc caaacatcac
    55321 gcctactttt tcacggattt ggttgatgaa aatagcgatt gtttttgttt tgttgatcga
    55381 accagagagt ttacgcaatg cttgagacat caaacgtgct tgtaacccga catgtgagtc
    55441 acccatttcg ccgtcgattt ctgctcgtgg aactaaagca gcaactgaat cgactactac
    55501 gatatctaca gccccacttg atactaaagc atcagcgatc tctagacctt gttctcctgt
    55561 gtcaggctgt gaaagaagta gttcatcgat attcacacct aatttttgtg catattgcgg
    55621 atctaacgca tgctcagcat caatgaaagc ggccgttccg ccattttttt gtacttctgc
    55681 aatagcgtgt agtgcaactg ttgttttacc tgaactttca ggtccatata cttcgatgat
    55741 acgtccacgg gggtaaccgc cgacacctaa agcgacatct aacgcaagag agccacttgg
    55801 aattgtagag atttgttggt cgattttttc gcccaatttc ataatagagc ctttaccata
    55861 gctcttttca atttttttca gtgcagcatc taaggctgct ttacgatcat ctgccaatac
    55921 gataatcctc cttatattta caaatcagag caacatttca ttatccttat catatctttt
    55981 ttagtgaaaa aaagcaagaa aaaaccgaac aaatattcgc atttatcatt ttcttaaaag
    56041 ctgttttctc aaaagatcaa atccttgcat aaccgcatta tatctgatag cagcacgatc
    56101 tctggcaaag tgatattttt ccgaaaccac gccgtcttta gatgaaatag cgatccatac
    56161 tgttcctgca gattgacctt ctaacgtgtc cggtccagcc actccggtga aagctacact
    56221 gtaatctgtt cttgctatct cacgtgcttg aatggccatc tgctccacac attctttgct
    56281 gactgttcca tattctgcta atagctccgg atcaatctcc agaaaaccag cttttgtttg
    56341 gagcgagtag gtaacgaatc cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa
    56401 tgcggcttgg aaggcgccgg ctgtcaaact ttccgctgct gttacagtct gcttgttttc
    56461 tttcagtagt tccactacga cttttgctag cgaattatca tcgccgtatc cataaaaata
    56521 ttcccctact cgttcttgga ttttttcttc taatgctaaa agagcttgat tccctgcctg
    56581 aacatcattt gttttcactg tcagtcgtaa tgtcacttca tttggtttgg catatggtgc
    56641 gatcgttgga ttgatctgtg tctcaatcag atcttttagc tcagtgacca gtcgcgattc
    56701 accaatccca taaaagcgca aaactttaga aattagtttt tcttcagaag gaaaacgttc
    56761 ctctagtaaa ggacgtactt gttccacaaa cattggtttc aactcattag gcggaccggg
    56821 aagaaggata tacgcatgtg tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg
    56881 atttggtaga ggaaccccgc cttcgataat ctgggactgt tgcagattgt ttttggtcat
    56941 ttttcgatgg gttgtttcaa aatatgccaa taattttttg tatccttccg tattttgtat
    57001 gagagactta ccgaggtgag ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc
    57061 taatccgcca cataagagta tgagttcact tcttgtttca gctagagcaa tcaactcttc
    57121 taaacgctgc tgattatctc ctacaactgt ttgataataa acttcgatac ctagatcagc
    57181 tagctgttct gacaagaaag tggcattcgt attcacgact tgtcctagta aaagttctgt
    57241 tccgacagct attatttctg ctttcattac ttcccgctcc cttatatagt atacgctgta
    57301 tttttatatt tttttattta aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc
    57361 tccttgttcc aaataaacag tgttctcctg ccaaaacctc ggtcataagt cacaatatac
    57421 ataaaacatg aaaagctgtt ttttgaaatt atcccttgtg actcagagtt agacggcagg
    57481 ctcacaacct ctccactaaa cgatacttac attgatcctt tgaacacacc gctgtttttg
    57541 atgaagtaat ccacacctga atagatagta aagatcagac aagcataaag catgatttga
    57601 tctactggaa taccgatcag gctgaatggg atattattga taaacagcaa gataatagca
    57661 accatttgtg tagccgtctt gacttttccc ggccaagctg cagccatgac ttctccatgt
    57721 tcgacaagta gtaaacgcaa gcctgtgacg gctaattcac gacagacaat gatcgctact
    57781 acccaaccag gggcctttcc ttggtcaacc aacatgataa aagcagtcat aactagcatt
    57841 ttatctgcca acgggtccgc aaacttgcca aaattagtga caaggcctcg cgcacgggca
    57901 atttttccat ctaaccagtc tgtgatacta gctaaagcaa aaataactgc acctactagc
    57961 tgtgtgacag ccagtgtggt gtctcctacg ttaaattgtc cccaatctaa aggtaaacct
    58021 acaaccaaaa tgaaaatcgg gatcatacaa attctaagta cagttaattt atttggtaaa
    58081 ttcacactat cgactccttt cttccctatt ctacgtgact atcagcgaag tttccaccga
    58141 tattatttac ttttccaaat gaatttattc aaaggagtat ttttattgaa attgatcttt
    58201 ttgaattaac gaagaaggac cgcgacacag atttgtcacg atccttcttt tttataccta
    58261 caagtaaaca gatgcgtaaa ctaatttata cgatcattct gcgtattgaa gagtaaggtt
    58321 cacatttttt ggactctgat tgttttcccc tggattaact ggtacggatt ctccattcgc
    58381 tttgatcgtt gcattgcttg ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg
    58441 taacacggta ctctgtgttt catttgctgc cagtgtccct tgataaacat aggcattgtc
    58501 tactagcaca cctaaccata cacgattgac agcagtaaat tcaagggtta caggcttggt
    58561 agcgtctgtg atatttactg cgatggcact gccagaatct tctcctgtag ctattgccat
    58621 tttcttttct tcacttgatt ttgttgttga ttctactgta gaagactctt tggtctgcgt
    58681 gctttgctct gaggaagagg actcctcgac tgatccttct actttaagcg aagaagctgt
    58741 gtctccgatt attggatcag cttgacgatc ttgccaagtc atatagccca cgacaacgac
    58801 aatcgccaaa gcaaccaatc ctaataaaat gacaggaaga cttgtccaaa agcgacttgg
    58861 atgcgattct tcctgatgca ttgcttttct agaagtactt actgtttcag gctgcggacg
    58921 ttcagggacc gatcctgtca atttgctttt cccatcaaaa acatcgacta aatggtctcc
    58981 atcttctcct acagcttcag cgtattgccg aataaatgcc cgcacgtaaa atttaccagg
    59041 caattggtca aaatcatcat tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat
    59101 ctgctgtaat tcatctaaag atattttttt attcaggcgt gcttgccgta atttttctcc
    59161 gatggtttca aaagccactc gttcatcttc tccagtctgt ttgattctcc gaagcatctg
    59221 ctgttgcaac gaaagacgtt atgtttgggt gctgtgacaa aagtcatgtc acagctcctt
    59281 ttttccgaat aaacattgtt ctcctgccaa ctcctcggtc acaagtcaca atatccaata
    59341 aatataagaa gctgtttttt gaacttgttc cttgtgactc ggagttaaac ggcaggctca
    59401 cgacctcttt cttaaacggt gttcaatcag ctgttcctcg gtattagctc aaaaaccggc
    59461 aaaatataag gagatatttt tccgtatgtt tctctaatac tcaggtcaaa acgctgattt
    59521 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
    59581 gttaaaaaca ttaagaagct gtttttactt ggctgttctt attacttgat gcagggcagc
    59641 tttttcacaa cctcttggta cacggtgttc cagaagtagc ttcttcggca ataagccgaa
    59701 atatcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
    59761 acttctgtca caacctcttt gattacttcg tttgatttta ttttaatata agttgattaa
    59821 aagagagcat gatttgtttt attttctcac taatttttcc aacacaacta agaataccat
    59881 aaaagctgat tttggaggag gttcctacta caagtctaaa acgaaattta agacttttcc
    59941 tcataaaata ctggtttatt ttggtctcat gtaaaactcg ctgaaggcct cttcacttat
    60001 aaatgcttcc ccagctgcca gtacgtcttt catttggatc gaatcgatga tttcaggaag
    60061 atcaaataaa gttcgatcgc caaataaact ttgcgtaaat tgattggcaa tatattcgat
    60121 cgaatttaat gattggaaat attgtccaag cattttcttc ttcagcaaat cgagatttgt
    60181 ttctgacact tccggatcat cagcgaatcc aagaatgatt tctttgactt tttctgctgc
    60241 tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag tgaaattcac gatccaagct
    60301 gaattcaaaa ccaaagctgt catcgataat tccttgattg tacatcgcta gatagtttcg
    60361 agaagtattg cctaaaatca tttggaacaa cagattgatt gctgttttat agcgtaacag
    60421 ttctgttcct tcttgcggca atgtgtctaa tcctttaata ccaagtacaa atttatctcg
    60481 tgtaatggct gcttccaatg cactttgttt gatgatttct tttgtgtttt cagggaaata
    60541 acgtacaatt tcttgctttg gagggaaatt ttttgcttct tggttttccc gaataagttt
    60601 catcaatttc tcaggctcca tcttaccaac tacaaataaa accatattgc ttggctgata
    60661 aaacgtccga tagcacgtat acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt
    60721 gcccgcaata tcgatatgca atgggtgtgt aggatacaag ttatttagta tgccaaaaaa
    60781 catccgccag ttagggtcgt cttcatacat ttggatttcc tgaccgatga ttcctttttc
    60841 cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag
    60901 attcttttct acctgatccg tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa
    60961 agcattggcg gaagctcctt gtttcccaaa taattgaaag acatcaccat cttctttttc
    61021 aaacaactta tgttctaaaa aatgggcaat tccatctgga actttcacct tttctttttc
    61081 accataggga ataaattcgt tatcgatcga accataattc gtactgaaca aaccatatgt
    61141 cttgtgatag tcatttttag gcaacaaata cacggtcaaa ccgtttggca gtacttcatg
    61201 ataaagtgtt tcattgattt gctcatattc tgttttattc atttgcattt cctcctgcta
    61261 aaaagaagat tgcctgcaat tcgatttgct cagctacttg ttgtatctct ttgatcgtta
    61321 cgctctccat acgtttcaac cattcttcat cagataattt tgtttccggc agccagctgt
    61381 ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag agaaagcaga tattggtttc
    61441 tcagcattgc ttttgtctga gacagttcca actctgttat ttcaccattt cgcaaacttt
    61501 ctaattgctc gtgaatcaga tgaagtactt gatttcggtt cttttcatcg ataccggttt
    61561 ggacactcat gaatcctcgg aacgtatcca cgctactaga ggcataataa gctagacttt
    61621 ctttctctct gacattcatg aaaagtttag agtgaggaaa accgccaaat aaaccgttaa
    61681 agaccattag cgcaaaacgt tctggttcat cgtaatacac atttgtctga taagctaggt
    61741 tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc cttcacgata ttcacttgag
    61801 gttgggtata aaacagatct ggataaactg tttcccgcga ctcaaatggt aattgcccaa
    61861 ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac aaaaatgtcg atttgatcat
    61921 ttgccatcat ttcttgatag taggacgcta gcgaactagc agtgatcttg tccaaatctt
    61981 cgactacgcc caaccctggg atcttttgtt ccgggctgtt ttcaaaataa agttcttgca
    62041 aggcatagga agcaaaagtt tgtttatctt ccttcaaact ttccaaatat agacgcagat
    62101 tgttcttttc caagtcaaac gtcaactgat caaactgatg atttttaata tttggataaa
    62161 ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag gtttggatca ttgacatatt
    62221 caccattcac aaaactgatc cctgcgttta gccaatgtaa atttcctttt tttctaactg
    62281 ataatccaaa gcttgcgcca taaagctctg caagttgcgc acttaacttt gtctgatccg
    62341 aataattcaa actatttgtt tccaacatgc tgctgagaag tgaacgtttc gtgatgactt
    62401 cttgttgcag acgcgcagta aaacggataa aaatccggat cgttttgtat tgttttgttg
    62461 gtaagacatg aagattcacg ccttctgcca attgatacac catatttata tgctcctttt
    62521 cacagttgat tgcccgctta ctcttttaat gggtcaacct gcctttttta cttattcctc
    62581 tgcttgaaat ttataagttc aggcaattcg ccgttttttc taaatgatgt tacactttat
    62641 agtatagcat agatgttttt tatcaaaaga aacagacggc taaaagcgta tattgttttt
    62701 ttatttttta tttttcgata tactaaagac aaataaaaga agggagggtc attcatgatc
    62761 aaagaattca aagagttcat catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt
    62821 attggtagtg cgtttacaag catcgtaaac caagtggtgc aaggcttgat cacaccacta
    62881 ataggttggt ttgtcgcaat gatcacaggt acttctgatt tagaaggagc actttcaatt
    62941 cttgactggt cgccgacaaa aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc
    63001 attacatttc tgatcactgg ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg
    63061 aagaatttac gctccaaaga agagcaagtg gaagtggaag aaactcctac tgcagaagat
    63121 tatcttagtg acatacgcga tcttcttgcc aaacaagtgg cagaagacaa tacacatcca
    63181 gtagagcaaa caaatctttc cgatacttct aaataataaa gaatctaaaa aaaagaaagc
    63241 cgattttcag tgtcggcttt ctttttttag attcttaact tgctacttgt tcctttttca
    63301 tgtcgatatg tggaatatca tcttccaaat acacatctga gattgccttg aatccaaacg
    63361 actcataaaa agcttgtagg tattcttgtg cgccgatcac gattggtttt tctggaaaat
    63421 ttttctggat catgttaatc gtttcttgaa caatctttct tcctaaacca tctttacggt
    63481 tgtcctgatg aacaaggaca cgtccaaaat ggatactctc cgcttcttca tatactcgcg
    63541 tgtaggcaag gatcttccca tcagtatctt gaaaaaacgt atgccaagct tctccatcaa
    63601 tgtcatcaac ttcttgataa ggacactgtt gttccacaac aaaaactgcg attcgttctt
    63661 tgacgatcgc gtaaaactct gctgttgaca attcttcaaa tttctttacc tgatattcca
    63721 ctaagcaaac gccccctgct tttttattct tagcataccg attcgcacac aaaaaagcaa
    63781 acgaacctta cgaatctcag gctcgtttgc tttttaaact gcttcagtta aaaattcgat
    63841 ctcaactgtc cgagtaagta catctgaata gctgtaagat actcgttcaa aagaattttc
    63901 ttcaggatcc aaatcaacaa cgaatactga aggataggtt tctgttaaaa cgccgcggcg
    63961 ttctgtttga cgctttcttc ctgtctgagc aaccaacatg attgggctgc cgatacgacc
    64021 ttctaaatct ttcttgattg aagctaaagt tgttggcata cagttcacct ctactatcaa
    64081 ctataccaca tatttttaaa attttcaagt ttaccataaa aaaaaaagaa atgcaataaa
    64141 aataaataag
//
LOCUS       DAMYXM010000009        64119 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.009, whole genome shotgun sequence.
ACCESSION   DAMYXM010000009 DAMYXM010000000
VERSION     DAMYXM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.009"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(262..765)
                     /locus_tag="PNS21_000670"
     CDS             complement(262..765)
                     /locus_tag="PNS21_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDI5158402.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1124..2149
                     /locus_tag="PNS21_000671"
     CDS             1124..2149
                     /locus_tag="PNS21_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDI5158403.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2472..3350
                     /locus_tag="PNS21_000672"
     CDS             2472..3350
                     /locus_tag="PNS21_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HDI5158404.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            3826..3942
                     /locus_tag="PNS21_000673"
     CDS             3826..3942
                     /locus_tag="PNS21_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDI5158405.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            4870..5208
                     /locus_tag="PNS21_000674"
     CDS             4870..5208
                     /locus_tag="PNS21_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158406.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5302..5532
                     /locus_tag="PNS21_000675"
     CDS             5302..5532
                     /locus_tag="PNS21_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158407.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            5932..6303
                     /locus_tag="PNS21_000676"
     CDS             5932..6303
                     /locus_tag="PNS21_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDI5158408.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6577..7269)
                     /locus_tag="PNS21_000677"
     CDS             complement(6577..7269)
                     /locus_tag="PNS21_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDI5158409.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7272..8057)
                     /locus_tag="PNS21_000678"
     CDS             complement(7272..8057)
                     /locus_tag="PNS21_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDI5158410.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8294..9694
                     /gene="lacG"
                     /locus_tag="PNS21_000679"
     CDS             8294..9694
                     /gene="lacG"
                     /locus_tag="PNS21_000679"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDI5158411.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9719..10036
                     /locus_tag="PNS21_000680"
     CDS             9719..10036
                     /locus_tag="PNS21_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDI5158412.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10049..11722
                     /locus_tag="PNS21_000681"
     CDS             10049..11722
                     /locus_tag="PNS21_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HDI5158413.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            11736..13367
                     /locus_tag="PNS21_000682"
     CDS             11736..13367
                     /locus_tag="PNS21_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HDI5158414.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(13858..15423)
                     /gene="guaA"
                     /locus_tag="PNS21_000683"
     CDS             complement(13858..15423)
                     /gene="guaA"
                     /locus_tag="PNS21_000683"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDI5158415.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15482..16405)
                     /locus_tag="PNS21_000684"
     CDS             complement(15482..16405)
                     /locus_tag="PNS21_000684"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDI5158416.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            16601..17206
                     /locus_tag="PNS21_000685"
     CDS             16601..17206
                     /locus_tag="PNS21_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI5158417.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            17514..18530
                     /locus_tag="PNS21_000686"
     CDS             17514..18530
                     /locus_tag="PNS21_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDI5158418.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            18671..19972
                     /locus_tag="PNS21_000687"
     CDS             18671..19972
                     /locus_tag="PNS21_000687"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDI5158419.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            20018..20680
                     /gene="deoC"
                     /locus_tag="PNS21_000688"
     CDS             20018..20680
                     /gene="deoC"
                     /locus_tag="PNS21_000688"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDI5158420.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            20701..21099
                     /locus_tag="PNS21_000689"
     CDS             20701..21099
                     /locus_tag="PNS21_000689"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDI5158421.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            21165..22247
                     /locus_tag="PNS21_000690"
     CDS             21165..22247
                     /locus_tag="PNS21_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI5158422.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            22459..24021
                     /locus_tag="PNS21_000691"
     CDS             22459..24021
                     /locus_tag="PNS21_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5158423.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            24014..25147
                     /locus_tag="PNS21_000692"
     CDS             24014..25147
                     /locus_tag="PNS21_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5158424.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            25147..26103
                     /locus_tag="PNS21_000693"
     CDS             25147..26103
                     /locus_tag="PNS21_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5158425.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            26202..27368
                     /gene="deoB"
                     /locus_tag="PNS21_000694"
     CDS             26202..27368
                     /gene="deoB"
                     /locus_tag="PNS21_000694"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDI5158426.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            27392..28210
                     /locus_tag="PNS21_000695"
     CDS             27392..28210
                     /locus_tag="PNS21_000695"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDI5158427.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            28227..28934
                     /gene="deoD"
                     /locus_tag="PNS21_000696"
     CDS             28227..28934
                     /gene="deoD"
                     /locus_tag="PNS21_000696"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDI5158428.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(29232..29918)
                     /gene="gpmA"
                     /locus_tag="PNS21_000697"
     CDS             complement(29232..29918)
                     /gene="gpmA"
                     /locus_tag="PNS21_000697"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDI5158429.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(30090..30671)
                     /locus_tag="PNS21_000698"
     CDS             complement(30090..30671)
                     /locus_tag="PNS21_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI5158430.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(30804..31487)
                     /gene="rpiA"
                     /locus_tag="PNS21_000699"
     CDS             complement(30804..31487)
                     /gene="rpiA"
                     /locus_tag="PNS21_000699"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDI5158431.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            31806..32219
                     /gene="rpsL"
                     /locus_tag="PNS21_000700"
     CDS             31806..32219
                     /gene="rpsL"
                     /locus_tag="PNS21_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDI5158432.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            32303..32773
                     /gene="rpsG"
                     /locus_tag="PNS21_000701"
     CDS             32303..32773
                     /gene="rpsG"
                     /locus_tag="PNS21_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDI5158433.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            32867..34954
                     /gene="fusA"
                     /locus_tag="PNS21_000702"
     CDS             32867..34954
                     /gene="fusA"
                     /locus_tag="PNS21_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDI5158434.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            35126..36313
                     /gene="tuf"
                     /locus_tag="PNS21_000703"
     CDS             35126..36313
                     /gene="tuf"
                     /locus_tag="PNS21_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDI5158435.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            36720..37028
                     /gene="rpsJ"
                     /locus_tag="PNS21_000704"
     CDS             36720..37028
                     /gene="rpsJ"
                     /locus_tag="PNS21_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDI5158436.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            37059..37688
                     /gene="rplC"
                     /locus_tag="PNS21_000705"
     CDS             37059..37688
                     /gene="rplC"
                     /locus_tag="PNS21_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDI5158437.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            37716..38339
                     /gene="rplD"
                     /locus_tag="PNS21_000706"
     CDS             37716..38339
                     /gene="rplD"
                     /locus_tag="PNS21_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDI5158438.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            38339..38629
                     /gene="rplW"
                     /locus_tag="PNS21_000707"
     CDS             38339..38629
                     /gene="rplW"
                     /locus_tag="PNS21_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDI5158439.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            38668..39501
                     /gene="rplB"
                     /locus_tag="PNS21_000708"
     CDS             38668..39501
                     /gene="rplB"
                     /locus_tag="PNS21_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDI5158440.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            39544..39822
                     /gene="rpsS"
                     /locus_tag="PNS21_000709"
     CDS             39544..39822
                     /gene="rpsS"
                     /locus_tag="PNS21_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDI5158441.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            39845..40192
                     /gene="rplV"
                     /locus_tag="PNS21_000710"
     CDS             39845..40192
                     /gene="rplV"
                     /locus_tag="PNS21_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDI5158442.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            40206..40862
                     /gene="rpsC"
                     /locus_tag="PNS21_000711"
     CDS             40206..40862
                     /gene="rpsC"
                     /locus_tag="PNS21_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDI5158443.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            40865..41299
                     /gene="rplP"
                     /locus_tag="PNS21_000712"
     CDS             40865..41299
                     /gene="rplP"
                     /locus_tag="PNS21_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDI5158444.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            41289..41477
                     /gene="rpmC"
                     /locus_tag="PNS21_000713"
     CDS             41289..41477
                     /gene="rpmC"
                     /locus_tag="PNS21_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDI5158445.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            41502..41768
                     /gene="rpsQ"
                     /locus_tag="PNS21_000714"
     CDS             41502..41768
                     /gene="rpsQ"
                     /locus_tag="PNS21_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDI5158446.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            41826..42194
                     /gene="rplN"
                     /locus_tag="PNS21_000715"
     CDS             41826..42194
                     /gene="rplN"
                     /locus_tag="PNS21_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDI5158447.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            42231..42539
                     /gene="rplX"
                     /locus_tag="PNS21_000716"
     CDS             42231..42539
                     /gene="rplX"
                     /locus_tag="PNS21_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDI5158448.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            42567..43106
                     /gene="rplE"
                     /locus_tag="PNS21_000717"
     CDS             42567..43106
                     /gene="rplE"
                     /locus_tag="PNS21_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDI5158449.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            43125..43310
                     /locus_tag="PNS21_000718"
     CDS             43125..43310
                     /locus_tag="PNS21_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDI5158450.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            43346..43744
                     /gene="rpsH"
                     /locus_tag="PNS21_000719"
     CDS             43346..43744
                     /gene="rpsH"
                     /locus_tag="PNS21_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDI5158451.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            43776..44312
                     /gene="rplF"
                     /locus_tag="PNS21_000720"
     CDS             43776..44312
                     /gene="rplF"
                     /locus_tag="PNS21_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDI5158452.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            44473..44829
                     /gene="rplR"
                     /locus_tag="PNS21_000721"
     CDS             44473..44829
                     /gene="rplR"
                     /locus_tag="PNS21_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDI5158453.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            44850..45350
                     /gene="rpsE"
                     /locus_tag="PNS21_000722"
     CDS             44850..45350
                     /gene="rpsE"
                     /locus_tag="PNS21_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDI5158454.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            45365..45544
                     /gene="rpmD"
                     /locus_tag="PNS21_000723"
     CDS             45365..45544
                     /gene="rpmD"
                     /locus_tag="PNS21_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDI5158455.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            45588..46028
                     /gene="rplO"
                     /locus_tag="PNS21_000724"
     CDS             45588..46028
                     /gene="rplO"
                     /locus_tag="PNS21_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDI5158456.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            46028..47323
                     /gene="secY"
                     /locus_tag="PNS21_000725"
     CDS             46028..47323
                     /gene="secY"
                     /locus_tag="PNS21_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDI5158457.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            47385..48032
                     /locus_tag="PNS21_000726"
     CDS             47385..48032
                     /locus_tag="PNS21_000726"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDI5158458.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            48223..48441
                     /gene="infA"
                     /locus_tag="PNS21_000727"
     CDS             48223..48441
                     /gene="infA"
                     /locus_tag="PNS21_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDI5158459.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            48474..48590
                     /gene="rpmJ"
                     /locus_tag="PNS21_000728"
     CDS             48474..48590
                     /gene="rpmJ"
                     /locus_tag="PNS21_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDI5158460.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            48608..48973
                     /gene="rpsM"
                     /locus_tag="PNS21_000729"
     CDS             48608..48973
                     /gene="rpsM"
                     /locus_tag="PNS21_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDI5158461.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            49001..49390
                     /gene="rpsK"
                     /locus_tag="PNS21_000730"
     CDS             49001..49390
                     /gene="rpsK"
                     /locus_tag="PNS21_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDI5158462.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            49470..50408
                     /locus_tag="PNS21_000731"
     CDS             49470..50408
                     /locus_tag="PNS21_000731"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDI5158463.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            50436..50816
                     /gene="rplQ"
                     /locus_tag="PNS21_000732"
     CDS             50436..50816
                     /gene="rplQ"
                     /locus_tag="PNS21_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDI5158464.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            51088..51927
                     /locus_tag="PNS21_000733"
     CDS             51088..51927
                     /locus_tag="PNS21_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDI5158465.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            51903..52772
                     /locus_tag="PNS21_000734"
     CDS             51903..52772
                     /locus_tag="PNS21_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDI5158466.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            52762..53559
                     /locus_tag="PNS21_000735"
     CDS             52762..53559
                     /locus_tag="PNS21_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDI5158467.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            53762..54508
                     /gene="truA"
                     /locus_tag="PNS21_000736"
     CDS             53762..54508
                     /gene="truA"
                     /locus_tag="PNS21_000736"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDI5158468.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(54963..57632)
                     /locus_tag="PNS21_000737"
     CDS             complement(54963..57632)
                     /locus_tag="PNS21_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HDI5158469.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(57870..58058)
                     /locus_tag="PNS21_000738"
     CDS             complement(57870..58058)
                     /locus_tag="PNS21_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDI5158470.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            58328..58690
                     /locus_tag="PNS21_000739"
     CDS             58328..58690
                     /locus_tag="PNS21_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDI5158471.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            58742..60424
                     /locus_tag="PNS21_000740"
     CDS             58742..60424
                     /locus_tag="PNS21_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDI5158472.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            60541..62577
                     /gene="recG"
                     /locus_tag="PNS21_000741"
     CDS             60541..62577
                     /gene="recG"
                     /locus_tag="PNS21_000741"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDI5158473.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            62626..63627
                     /gene="plsX"
                     /locus_tag="PNS21_000742"
     CDS             62626..63627
                     /gene="plsX"
                     /locus_tag="PNS21_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDI5158474.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            63749..63991
                     /gene="acpP"
                     /locus_tag="PNS21_000743"
     CDS             63749..63991
                     /gene="acpP"
                     /locus_tag="PNS21_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDI5158475.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        20558 a        11500 c        13810 g        18251 t
ORIGIN      
        1 taaattaaga cggaattcct ttctagattg agatattgac taggaagaat gttaagattt
       61 taagccttta agctaaaaaa aaaccctttt tgttaaataa ggggaatata gcctgtcagt
      121 ttcaaactgc aagtcgtcct gctgcaataa tatagtagat tagagatgaa aatagatttt
      181 tttaaaccaa tgactgaata aaaagaccgt catagataca cattggaatg tgtctattac
      241 ggtctttttt tacgaacgat tttattgaag acggacagtt cgggtcaaaa ataaaaaagc
      301 tacgatcaga aataagaata aaatgtatcc caaaataatg gctagacggt ctaacttatt
      361 tgtcgataga tgtttgttta gaagttgata ggcccataaa cctatcagtc ctcctgttgt
      421 attagttatc aaatcagtga tgtccgctac gccaataccg aataggaatt gaatcaattc
      481 tgccaagaag cttacaagga aaataatccc taatttttta gaaaaagaaa gtctcttgaa
      541 attgacattc aaaagcaccc caaaaggaaa aaagacaatc aagttataag tggtctcacg
      601 taataccgtc gtattttcaa aagaaaaagt agaaaaaggg acgagattga cattgctgta
      661 agaataagtg agaaattgtg gaagattaaa tgatagcttg aataaaatca accataatag
      721 aaagaataaa taaatcccta ataaaaattt tgatacatac cgcatagaca tctcctccat
      781 ttaaaaagta taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa gtttaaggac
      841 caacgtagtt atcgtacgaa aattagaagc ttttttttgt tgggaatccg gtttctttaa
      901 caaagctaag atagcgagtt gcttggaaaa gttgctatct tttttctgtc aaaacgactc
      961 ggaatgttcg ttttttgttc taatttctcc aagttgttta ctttttgata ctaagtttat
     1021 gaaaaatcaa gaaatagttg ttatactagg taatgctgac aaacgggatt ggcatttcat
     1081 cttggttcag ctttttttcc aattactagg aaggagggat agattgttca agacttattt
     1141 tcgtttcaat aaaatacaag ataatccttt tcgtatcatg aatctgatgc tacttatctt
     1201 tattgtatta tggttaggtt acataaatca tgattttttg atttcaagtt tttcatcatt
     1261 agggatcttt acttttttct attatcaaaa cataccgatg aaatatttgc tgaaacgtat
     1321 gtttctgatc ggttgcggac tgttggttgc tttcgttttg gggatcttat cgacttatgt
     1381 tttatggctt gaaccatttg ccgtagcttt ggttgctttt tccagcagat tcgtgttgcg
     1441 attattccat atttctaaac caggtggctt gttttttgcg atgctttctg ccatgggaac
     1501 gagcatgcag ctgccaatcg ctcaattacc gatcgtctca ctttattttt ttatgggtgt
     1561 cgtttttgcc ctgattgcgg cagtgattac aaaattactg gattcacggc ctgagcagac
     1621 aattgaaaaa gcaactttaa aagaaagatt tcacgaagag cctttagtga tcattgattc
     1681 tgttttctac agcgctgcct tatttctttc cgtttatgtc agtcacggct tgaatctgca
     1741 taatccttat tggttgacct tgtcatgtgc atcgattttg ctagcagaaa acttggatgc
     1801 gatgaagcat cgacaagtcc aatatctgat tggatcgatg ggtgggttat gtgtgtcggc
     1861 attcttgtca tttgtgccgt ttacgcagtt gcagaccatt tttctgatca cttttctata
     1921 tggaatcgca caatttctag ttgctagaaa ttatgcagtt gctaatattt ttcttaatcc
     1981 gatggcgttg atgctatcta cacttattcg tggagcatac ttgatttctt tgatcgaata
     2041 tcgtttttta gggatcgtca taggaagttt catcggttta ggtgttgcgt gggtaatgac
     2101 ggttggtttg caacattatt taacggttgt tcgtcaaaaa ttagaataga aggacaaata
     2161 tatgtgccaa aagaggttat gacaagattt ttgtcacaac ctctaattca aggtgttcaa
     2221 aagccagatt ctcagcatta agtcaaattc acacaaaaat gtggaaagga actttcaaaa
     2281 aaattttata tttattctat caaaaaattt tgtttatgat ggtaactgaa taaactttcc
     2341 tgagatggtc aatgctttcc ttgaaagtct tacagatatt ctttcttcat agaaagttta
     2401 agttttatag ttagtgtaca ataaaagtat gtttatacca tatggtgtcc ggataatcag
     2461 tcatttaaac aatgaggttc aaagatatga aaaggggaaa tttgatgaag aaaataagca
     2521 atgaaatcca aattctggca gaaactttaa ctcttcccaa agtcgttata gagcaaatga
     2581 ttgtacaagc aaaaaacatc cttgatgagg tgtttgagca atatagtcat gaactaatgg
     2641 atcctaataa agcaaaggcg actgcaaaga aaatagacaa agtctatgaa gaggaacctt
     2701 ttgcagcatt agctatttat ctatatgcag cttctcaaag ttggaaaaaa atttatcaac
     2761 cattgaagat tccaagaact atctatttgg caacaatgaa tgcttttact agatttattc
     2821 atgaacattt ccaagtaaca ggaagctatc gctttgaccg tggtttttgg atttggcatt
     2881 acactagtgg cttgatcttt cggattggtg aattggagta cgaaatgaca tattttccga
     2941 aagggtacac ggatctaaga ttagaaggaa agcagtgcct ttctcttcat attccttccg
     3001 atgcagattt atctcagaat aagataagag atagttacga aagtgccatt cgtttttttg
     3061 aactttattt tcctgattac cattaccaag tgatgtacac agatacttgg ttactatcac
     3121 ccaatcttac taaatggttg aaaaaagagt caaaaatctg tctttttgca gccgattatc
     3181 gattattatc agttgatgaa caggacgata gtggagtccc ttggatcttt ggacgagtag
     3241 atgcccaaat acatgattat ccagaaagta cgagtttaca gcgacaagca aaagagcaat
     3301 tgcttgcagg cgagcacatt ggctctggct taggtattct tacttcataa gtttagtact
     3361 tcgaaatagt ttaaaagagc gtgagacaaa agtaaagaac acttttgtct cacgctctaa
     3421 acacgtataa acggcggaag cagaagcaac tccttcggaa ataagctgaa attcacaaaa
     3481 atttgaaaag caattttcgt gaatttcttc ttatttcttg gagttaaacg cttctgtccc
     3541 agcctctttt ttagtgttct atcgaacaca cagtacgttc aataggtaaa ctagctataa
     3601 caatagatat taaagttaac ggacaagagg gcctttttta ggtagctaaa acgtaataat
     3661 gaaaatttat ttccagagat aggagtcaat cagctagtct gatcgactgt tttaacaagt
     3721 tgactttcat tttaactttt gataccattt aaaacacaaa tcgtaattgt ttcgatttgt
     3781 gttttttagc atgactacaa tcaaagaaga aagtgggtcg tccatatgga aaaaacagat
     3841 atctcaagtg cataccgccg cttgaaaagc ccaaatatca aaacacgaaa acgtgcgctg
     3901 aagatcatca aagatgtgaa acggaatagt gggaaaaggt aagaagaagg aaacttaatt
     3961 aaaaaaatag ccgaaaaacg tttttcggct attttcaata atcggttttt gtgtaaggtt
     4021 tccgaaagaa ctgctgttga ttttccattt ttaacatatg ataaattggt agaatctttc
     4081 ttttcaaatt gataccattc cctaattcca gttatatagt taactacaga accacaaagc
     4141 acgtacgttt ataaaagtat tatctgtagt tatttttcaa ttccctttca acctcttatt
     4201 ttgaccagta gtattgatca ctttaccttt taacaccttt gttcgttttt gaaacatttt
     4261 tagtcaagaa tggaaacgta actacataat caaaggtttt gcgaaatatt tcatgaaata
     4321 tttcatgtag aagcgagcaa tacggttgca aataaccatt atcgtttaat aagcagttac
     4381 gaaagctccc aatatctcta acgttcagct cactccattt cttttcttca aaaaaatcag
     4441 tgattcgtat ctctttctat ttgtcgaaaa agttaacttt aaaaagtaac gccgaaaaaa
     4501 tatgcaaaaa aataaaaaag cagttactat tgctagaata acatcaagga ataataagtt
     4561 ttggttttca gataatagtc gataagttgt gtattggaat tttagataca gtaataaagt
     4621 atttttcatg aaattttgtg agaatcacta ttacaagtat ctttaataaa attaatggtt
     4681 aaggaggaac ttaaagtaga atgtaaatga atagttgtag tcagtcgatt gtgatactag
     4741 actcaaagtt gcaggaataa gtttttcaat ttgtgattgg aagataaaaa atgaaaaaga
     4801 agggcttttt tttatacttt gttgattgta gtgtttattt tccctctgtt tagccaagta
     4861 acattttcta tgactcaacc agtttcaagt gaggaacatg ataacctttt tcgtgctaca
     4921 gaagagttta agaaatatca ggaaaatatt gttccggaga ctccattagc tagtgagcca
     4981 attactgata ttgatggaaa ccaaatagga tggtatatac agtacgaagt tatggtggta
     5041 aataatgact caatagtaaa aaatacagta ggcttagagc taacaccgat tgaaaaggtg
     5101 agttcattgt taacgtatat ttatactgat gaaagtgacg agactgagat cattttaacg
     5161 gattactcaa aaattgctac tgataaaaag tatatattgt ggatgtagac actagtgaaa
     5221 tactttatga tatagaaatt gatgaaaatt tactagatgt agcacaagaa atcgatcatc
     5281 aaaagcagga agctattcga tatgctggta gaaaagggaa aagaaaatat ttattacgtg
     5341 aatgtagcaa aagttagaga agatgaaaat gagtggaaag aattcaagag tcgctactca
     5401 attaactcga ctccgacatt tacagtttac agagaaggat cgatagaaaa aactgttttt
     5461 tggactaaag aaagtggaat gtctttagct gaagtagaag agtttttgga ttatgttagt
     5521 atgcaacagt aagggagttc ttattatgaa aaattttgac acatattatg ttattgtttt
     5581 ttcgatatct ttgctgttaa tattactaac tttaatgcag tataaagagt atccagagta
     5641 ttttatttta acttttgtat atttagtaag tagctacaca cttctatata aattcagaag
     5701 ttcttctaga gtttctaaag tttgtttctt aattagctta ttgattctat aatatttttg
     5761 atttatatag gaaattaaaa atattataga atcaacactc agagaacggg ttggttttta
     5821 atagatttaa taagtactga taatgaaatt tctcctggtt gggaaaacga agttggttac
     5881 cctgtagaag aattttttca agattacttt gcggagtatg ccgataggat aatgagagat
     5941 tacgtaacag ataaaactca agaagtcatc aatcaatccc agtcattaaa tgaattatct
     6001 gataaggcga aagaagaagc agttgccatt atttcttcag ggattagtga ctatattgat
     6061 atgagtggtg aaatcaatta tggcgataaa gacaaaattg tacgcatgat aaaagctttt
     6121 atcaaaactt ttgatgtgaa ggcagatctt aagtttaatg atacttacct agtaggaaaa
     6181 ttaattaata tccttgggac agagaaagat acggaaacgt ttatttcctc tttgtctatg
     6241 ttgtttacag ataagcattt ttcaactcag cttggtggag aggaactagt ggaaatcttt
     6301 taaccattac gcccagcatt aataaaagta tatcaaaaaa gtactcctga tgatttttaa
     6361 gattggtttg agaaataatg tgaaaacact atttttaatg ttaattatag atagagatga
     6421 acgcgtcata tggttaaaag aatgaaaaag tcaaagatac actgctatat ttcactaagg
     6481 atatagcagt gtatctttca tgtcgtttca ctggcagttt tttataaaag gaggtgtgca
     6541 tttcagttgc ttttctttgg tagttagttg aatcgtttaa gaattaacgg atgtggcaga
     6601 caggtgctct gtccaaaaac cagtaaaatc tttgatggtt ccatctttat ttagctgtgc
     6661 ctgttcaaat actgttttgt ttttaccgaa tggatcaatt gcaataattg ttccgatccc
     6721 cgcacgtttg gcagcaagta atcctgagta agcatcttcg ataacaagac aatcagccgg
     6781 agctaagctc agtttgtctg ccgctttaag aaaaatatcc ggttgcggtt tcccagggaa
     6841 agagccgtcg tcatagacaa tcttgtccca gtcaaaccag cgggctaaat cgaaaatatc
     6901 aaaataaaaa gcaacattct ctttgacagt agccgtggca atcgttaatg gaatctgttg
     6961 gtcagtcagc tggtcaagag tcgcttcaag cccttttgtc aactgaagct cgttttcatt
     7021 ttgcaagcaa agcatacgat aatagctttc tttttcttct gacaatacgg cgatctcaga
     7081 ggcggtcaaa tgctctgaaa tgaaatgttt caaaatctcc gtattcgtcc gaccatgaat
     7141 attttttaaa atatcctcgt ctgataaacc atcttttgta tgatcatgta tcatatgcag
     7201 ccaagcagct tcatgcagat gagagtccaa aaacattgtt ccgttgaaat caaatataat
     7261 ccctttcata gttacgctcc tatagtggta ttttcggttg aatcagcaat ttgcagatcg
     7321 acatattgtg catagtgctc ttgaacctcc cgcgagatct ggcgatccgt aatgagcaga
     7381 tcgaaatcat gcaaattata aaacacataa aaatcatctc gattgaattt tttattatca
     7441 acgagtagat atttcttacg ggaattattt agagcgatct gctgtgcttc tccttcttcg
     7501 gtactgtaag tagagatcgc ttcgttgtga acgccatttg aactgataaa tgcttttgta
     7561 aactttagtc ttcttaaatt gtcgttggtg atcggtccga caaatgcgcc ggtatttttc
     7621 cggaaatcac cgccaatcag tagaatttct ggtccatctg cttgagcaag aatatcaaaa
     7681 actggataat tatttgtaat gatccgaatt ttcttgttca gtaaatgcgt cgccaatagt
     7741 tcgattgttg tacctggtcc taagaaaatc gtttctccct cgtgaatcaa ggaggctgct
     7801 gtggcagcaa tttctttttt ttcttgaatc tgcagagtgg ctttttcagt gtgagaaagt
     7861 tcttgatcta aagtgtagga aagactttga gcccctccat ggacacgcag cagcttgccc
     7921 gatttttcta attcatctaa atctctgcga acggtcatat cagaaacatc taattgattg
     7981 atgatttcat taacggtaag gattcctttt ttattgacca tttctacgat agtaagcaat
     8041 ctttcttttt tcaacattgt cttcacctct aatttaagga taatctatca tataaacgga
     8101 caacagtcaa tctaattcga acaaaagtaa acatttaggg tggtttttat gtttgttaca
     8161 atagatttta ttgctgtttt ctggttttta tgattatttg tgtttgtttt tgttgacaaa
     8221 tgttttggat gcgtttacaa tgcaatcagt tagaaagagc gaaaactgat tatagatttg
     8281 aggagagatg tacatgctta aattaccaga agattttatt tttggcggag ccacagcagc
     8341 ctatcaggtt gaaggagcca ctaaggaagg tggcaaaggc gctgttgctt gggatgactt
     8401 tttagaggaa caagggcgat tcagtcccga tccggcaagt gatttttatc atcaatatgc
     8461 aaaggacatt gaattgtgtg aacgttttgg tgtaaatggt ctaagattat ccattgcttg
     8521 gagccgcatt tttcctgatg gagctgggaa accaaatcca gaaggaatcg ctttttatca
     8581 tcgtgtgttc gaagaatgta agaaaagaaa cgtaacgccg tttgtgacgt tgcatcattt
     8641 tgatacccct aaacgtttgt ttgaccatgg agattttttg aatcgagaaa caatagaagc
     8701 atttgtttcc tatgccattt tttgttttca tgaattcaaa gaagtcaagg tgtggagtac
     8761 ctttaatgaa atctatccag ttgcaaccaa tcaatatttg ttaggtgttt tcccaccagg
     8821 aatcaaatat gattttacaa agatcgtcgc ctgtcttcat aatatgatgg ttgctcatgc
     8881 ccgtgtagtg aactatttca aagaaaacga acttccgggt gaaattggtg tagttcactc
     8941 attagagacg aaatatgcgg caacagacgc gccggaagat aagcatgctg cctttttaga
     9001 cgatgcattg tcgatccgtt tcttattaga tgctacctat ttaggctatt attcaactga
     9061 aaccttgact gctcttgatg agatatgtga agcaaatcag gccagctatc attttcctga
     9121 agaagatttc gttgaattga aaaaagcatc cacgcgtaat gactacttag ggatcaatca
     9181 ctaccaatgc cattttgtca aagcttacga tggagaaaat gcaatccatc ataacggtac
     9241 gggtgaaaaa gggacctctg tttataaagt gaagggaatc ggagaacgta tttataaaga
     9301 aggaattcct cgaactgatt gggactggct gatttatcca gaaggtctat atgacttgtt
     9361 gctgcgaatt aaaagcgatt acccacatta caacaaaatc tatatcactg agaacgggat
     9421 ggggtataaa gatcagtttg aagatggcat tattatggat cagccgagaa tcgactattt
     9481 gagagtctat ttggaatcct taagcaaggc gataacagcc ggggtaaatg tgaaggggta
     9541 ttttttgtgg tcgcttatgg atcttttctc atggacaaat gggtataaca aaaggtatgg
     9601 cttgttttat gttgattttg aaacccagaa gcgttaccca aaagaatcag cttactggta
     9661 caagttggtc agtgaaacca aaacgattat ttaataagtg ataatggagg aagaaaagat
     9721 gaataaagag gaattacaaa tgcttggttt tgagatcgtt gcatattcag gcgacgctcg
     9781 cagtacatta ttgaaattat tgaaagaagt aagacaagga aactttgaac atgttgattc
     9841 ggcgttgaaa gaagccgacg aaaatttaac ccttgcacat aattcgcaaa caaaaatttt
     9901 agcggaagaa gcatctggga aagagatgga gatgggattc atttttatcc atgggcagga
     9961 tcatttaatg accactttat tgctgagaga tctgattcaa gactttattg tactgtacag
    10021 acagaatcaa ggataggaga agaacgctat ggatggaatc attaaacaaa tcgaaaaagg
    10081 gaaaccattt tttgaaaaaa tctcacgtaa tagttattta ggagccgttc gtgacggatt
    10141 tttaacagcg atgccagcta ttttattttc aagcattttc attttgattg ctgcggttcc
    10201 tgagattttt ggttaccaat ggcctgaatc tgtctccaca tggctatgga aagtctataa
    10261 ttattcgatg ggtgttgtag ggttactcgt ttcagcaacc actgctcgct gcttggctga
    10321 ttcgatgaat cgtcgcttac cgaaaaacaa agtaatcaac accacctctg tgatgctggc
    10381 ctctgtggta agtttcctgt tgttaagcgt ctctccgatt gaaggtggat tttcaacgga
    10441 atatatgggg acaaagggac tactggcttc ctttgtcgct gcatttttaa cagttaattt
    10501 atataagttt tgtgtgctga aagatattac cattaaaatg cctaaagaag ttcctggaac
    10561 gatttcgcaa accttccgag atatttttcc tttctctttt tcagtatttg cggccgtttt
    10621 aattgattta ggcattcgac aaatcttcca aatctcattt gcagaagcaa tcattacggt
    10681 gctgcagccg ctatttacag cagctgacgg gtacttagga atcgctatta tctggggagc
    10741 gatggcgctg ttttggtttg tcggtgttca tggaccgtcg atcgtggagc cggcaattgc
    10801 ggcaattatc tatgccaatg tggaaaccaa tctgcaattg ttcaattccg gtgaacaagc
    10861 ttctaaagta ctgacggtgg gtttagggaa ttttgtagga actatgggag gaactggggc
    10921 aacattggtt gttcctttta tcttcctact ctttgctaag tctaagcagt tgaaggcagt
    10981 tgggaaagct tcctttattc cagtaagttt cgctgtcaac gaaccattgc tatttgcggc
    11041 acccatgatt ttgaaccctt atttttttat tccatttttg ttaacacctg ttgtaaacgt
    11101 ttgcctgttc aagttttttg tcgatgtatt gggcatgaac agctttatgt acgttttgcc
    11161 ttgggcaact ccagcaccga tcggtttggt tctaggaacg ggcatgggta tcttagcttt
    11221 tgttctagca gcggttctaa tcgtcgttga cttcctgatc tatctaccat tttgcaaagc
    11281 atacgatgct gtcttagtag agaaagaagc acaaaaagca gaggtgatcg caaccgagga
    11341 aggtgcttct gccgatgtac gtgaggaagt acctgtggca gcgagtcaag ttatcagcga
    11401 tgcaaataaa gaaatcaatg tgttggtact ttgtgctggc gcagggacaa gtgcgatgtt
    11461 agctaatgct ttaaaaaaag gtgcatctga atcgaaagca ccgatcagtg caatggcggg
    11521 tgcttatggt tctcattacg aaatgctgca ggattttgat atggtggtct tagcaccgca
    11581 agtcaattct tactatgatg atatcaaaga agacaccgat cgcttaggaa tcaagctggt
    11641 tgcgacaaaa ggagcagaat atatcggatt aacgagagat cctgaaaaag ctgttcagtt
    11701 tattctgaac caatttcaat aaaggaggag ccataatgaa aatgggaatt tggttgcgta
    11761 aagctgtttt atttgcagct gtgttagggg ctttgggggc tgtttcaaaa actgttgaag
    11821 catctgccag cgaatttatt cgcggagcag atatttcgat cctgtcagat atggaaaaat
    11881 ctggtgcaaa atattatgaa gacgatattt caaaagatgc cttagagatt ttaaaaaata
    11941 atggtgtcaa ctatgttcga ctgcgtttgt ggcaggatcc ttataatagt aatggagaaa
    12001 gttatggagc agggacaaat gatctggaaa cgacgattga ccttgcaaaa cgagcaaaaa
    12061 atctgggact taaggtgctg ctggattttc actacagtga tttttgggtt gatcctggga
    12121 aacagaatct tcctaaagca tggcaaggtc tgacctttga agaaatgaat acggcattgt
    12181 acgactatac aaagaacgtt ctgtcggaaa tgaagcagct ggacgtttat cctgatatgg
    12241 ttcagatcgg caatgaactg aatagcggaa tgctctggcc ttatggcaag tcttggggag
    12301 aaggcggggg agaatttgat cgattggctg cttttttgaa gtctggtatc caagctgttc
    12361 gagacactca acctaaaaca actccagtaa tgctccactt agctgatggt ggcgatacag
    12421 gagcatttac ttggtggttt gatgaaatca cgagtcgagg tgtgtcattt gatttaattg
    12481 gagtgtcgta ttatccgtat tggcacggtt caatgaatga acttcaaacc aatatgaatg
    12541 cgatcagcca acggtatggt aaagaagtga tcgtagttga aacagcttat gggcatacaa
    12601 cagcgaatgc cgatactatg ccaaatgctt ttggtgaagc cgaagcagct gcggggggat
    12661 atgaaccaac accgacagga caagcagagt atttgctgga tcttgccgat cggattcagg
    12721 cagtaccgaa taatcgcggg gcgggcttct tctattggga acctctttgg tacaacggaa
    12781 atgtgagttg ggcaacacaa gcaggtatga attatctcag tgttcaatcc actatgggaa
    12841 acgaatggga caaccaagcg atgtttgatt ttgctggaaa cgccttgcca agcttgcgtg
    12901 cgtttaaaca agcaggggcg caaataaatc tagttaaaaa tgcaagtttc gaaagcgatg
    12961 gaccaacgac cagtccttcc aattggcaaa cctggtttca acaaggaacc gctaagggta
    13021 cgatcaaaac ggaagcgggc actttttttg gggactacaa actaactttt tggcaggatc
    13081 aagcatacga ggcatcagct tatcaaaatg ttagtggcat aagcaaagga acttacacat
    13141 tgagtgccta tgtaatgtct gggggagagt ttgatactaa tcagttatat atcaaaaatt
    13201 atggtggagc ggaactaaat actgcggttg ttaaaacaag tgaatggaca aaaattacaa
    13261 tcaaagacat tccaattaca acaggtgttt gtgaaattgg ggtttatagc aaggcttcag
    13321 gaaatgcttg gacgaacgtt gattttgttt cattggtgaa acaatagact gcggatcgtt
    13381 tcgattcaag atcggaggtt cccttcagcc acatcgttag cgtagaaggc aggaatcgaa
    13441 cctgcacctc gcctttgcca aaaataacca gaaacgattt ccagtcattt ttaccaaaac
    13501 gagtaaagca ccagacttca tctacacttt gtattatgct aaagagagtt caacgatttc
    13561 gtaagaagca atgaaaaaat tgcaaaaagt tgtgcagata acataaagtt tgtttaagaa
    13621 agggtaacct tagaattccg tcgtgaatat gatttgagca cttagatgga tagaaaaaag
    13681 ttctcggaaa acagttcgtt tttcgagaac ttctttttat gataagaatg aaacaaatta
    13741 acttatatta tagtctcttt tggtagggaa ccatcttatt tttaatgaaa atcaagcatt
    13801 ttagctattg aagaatgttg atttataggg attccccctc catattatgt tggctcttta
    13861 ttcccactcc actgttgctg gtggtttaga agtgatgtca tacacgatac ggttgacgtg
    13921 ggctacttcg tttacgatac gtacagagat tttttgcaag acttcccatg ggatacgggc
    13981 aaagtcagcg gtcattccgt cgattgaagt tacagcacgg ataccgactg tgtagtcgta
    14041 agtacgtccg tcacccataa caccgacaga gcggatccct ggaagaacag tgaaatattg
    14101 ccagatatca cggtcaagtc cagcagctgc gatttcttca cgcaagatag catcagattc
    14161 gcgtacgatt tctagcttgt cttcagtgat ttcgccaaga acacgaatac caagaccagg
    14221 accagggaat ggctgacgcc aaacgattga atcaggcatg cctaactgag tccctaattc
    14281 acggacttcg tctttgaata atgtgtttaa tggttcgatc aattcaaatt gcatatcttc
    14341 tggcagtcca cctacattat ggtgagattt gatcgtctgt gctgtttctg taccagattc
    14401 gataacgtca gtatacaacg ttccttgtgc taggaaagag acgccttctt caccagcaag
    14461 ttttgttgct tcgtcatcaa aaacatatac gaattcattc ccaatgattt tacgtttttg
    14521 ttctgggtca gatacacctt tcaacttgtc taagaagcga tctcttgcat tgactttgat
    14581 gatgttcaag ccgaattttc cgcctagact ttccattact tgttccgctt cgcctttgcg
    14641 tagcagaccg tggtcaacaa agatagatgt caattggtca ccgatcgctt tttgtaaaag
    14701 aaccccaacg acactagaat caacgccacc tgaaagacca agcaagactt ttttgtcacc
    14761 aacttgttca cggatttttt ctacttgcat atcgataaag ttatccattg tccagttacc
    14821 ttcacagtga caaacatcaa aggcaaagtg gcgcagcaat tcgattccgt attcggaatg
    14881 acgtacttct gcatggaatt ggataccata aaaattacgt gttgtgtctt ggatagaagc
    14941 aatcggacaa tctttgcttg ttccgactgt ttcaaagcct tcaggaactt tagttaccaa
    15001 gtcaccatgg ctcatccata ctgtttgttg ttttggtgtt tctttaaaca agacagcttg
    15061 atcgcttgtt acttctaaca aggctttccc atattcgcgg tttttagcag gctctacgga
    15121 accgccaagt ttgtacgtga tcaattgcat accatagcaa atccctaata caggaatacc
    15181 taaattgaag atctcaggat cgatcccaaa tgcattctca tcatatacac tgtttggtcc
    15241 gccagaaaga atgattccct ttggattcat cgcttttact tcttctgcag taatgcggtg
    15301 gctcaataat tcggaaaaga ctccaagatc acgaatacgt cttgtgatca attggttata
    15361 ctgactaccg tagtctaata cgataatctt ttcaacgttc gtcatatcgg caacgtttgt
    15421 caaatttatt acccctatcc ctttaaattt tatctcaaaa tagtcaaact attttaaaat
    15481 gctaatattt tcgcatgaaa atcgaatcga tgatatgatt ttcagtttta tggagaatta
    15541 tatcggctct tcctcgtgta ggcaagatat attcctgtaa atttttcaaa ttcacatttt
    15601 tccatacatc ttttgccatt ttaaaggctt cttcacgatc tccgatagca aaacgataat
    15661 aataattctc tggattttgg aaagccgtat ccagtaacgc accaaaacga tccaaatacc
    15721 atttttcgat cagcacggga tcggcatcga catagacaga aaaatcaaaa aagtcgctga
    15781 cgtagatttg ctgattcgca ggtaattgaa gcgtattgat cccctcaacg atcaagatat
    15841 caggctgctg gatcaattcg tacttgtctg gaatgatatc atacacatca tgtgagtaaa
    15901 caggtacttt tatctcttcc tgaccagatt ttacacggtt caagaaatcg atcagcatct
    15961 ccatatcata actttcagga aatcctttac ggtccatgat tccacgctct tttaatatac
    16021 gatttggata aagaaatccg tcagtcgtga tcagctgaac gtttcttcgt ttgaaggtac
    16081 gagacaaaat cgtctgcagc agtctggctg ttgtactttt tccaacggct acacttccgg
    16141 cgatcccgat aataaaaggc ggtacttcta cgtatttatg aagaaataaa cctttactca
    16201 agaacaaaga ttcaaattct ttcatataaa gataaatcag atgagcaaga ggtacataga
    16261 tatctcgaac atcctgcatg gaaatgcggt cattcaaact tttgatatta tctagttcat
    16321 cttgtgtcaa aggtgcttgc ccattacgat aaaattctcg ccattcttct cttgccacac
    16381 ggtagtagtt cattggatca ttcatattag cctcctgcgt tctcccatca atggaactat
    16441 tttaacacaa aataaacgca aaccccacca cttaagagag attgccgatc tttttgtata
    16501 atctttataa tagaaagaaa cggagtctta ttcttgactt gcgcacggat acataaaagg
    16561 atagactgtt gtcatgaaag aaatgagaag gatggaagac atgacaaatc attactattc
    16621 tgaacgccca gatacggcgc acgattttga acaatgggct tttgagttaa aaggcagaac
    16681 ctttcaattt gtaacagatt ctggtgtatt ttcacgtgaa acagtggatt atggttcacg
    16741 tgtcctgatt gatgctttcg attgggaaga gttacctgat gaaggaaaga tcttagatgt
    16801 gggatgcggg tatggaccga ttgggttagc tttggcattt gcgacacagc ggtttgttga
    16861 aatggtcgac attaatgaac gagcggtaag tctagcgcaa ggaaacgcaa agcgtaacca
    16921 aatcgaacat gttgatatcc atcaatcgaa tatctatgaa gcagttcatg aagaaaccta
    16981 tgcagctatc gtaagcaatc cgccgatccg tgctggaaaa aaagttgtac atgaaatttt
    17041 gacaggcgca tatcctcggt tgaagaaggg cggtacactg acaattgtga tccagaagaa
    17101 acaaggagcg ccgagtgcgc aaaagaaaat ggaagaaaca tttggtaatg cagaaatcgt
    17161 cacaaaagat aaaggatatt atatcatcaa aagtgtgaaa gaataactag ctgaaaaata
    17221 gtggagcgac gaaagagtgg aactgggcgt gcatgtacgg gtacgtatca gttctttttt
    17281 tgttgagaag tttttaaaaa caaagaacta ttttttcttc aaaaagaaat agtaggaaca
    17341 gagtcgttgt tttttacgat aaaataaata agatacagaa cttgtataga aggctgccaa
    17401 gtcggtagat aaaggaggtt tttatcaaaa aacatacttt ccttttttct ctattctttt
    17461 cttgggggtt ttggtacact gatagggaat ttagatagaa gggagccaat aggatgaagc
    17521 tgaccatcag agatatcgca gaaatggcag gcgtatcagt aacaactgtc tcacagattt
    17581 tgaataacaa aggaagcaga tttagtgata agacaagaaa aaaagtgttg tcgatcgtga
    17641 atgaacatca ttataagccc gattatttcg cttcaaatgt gatcaatcga cattcaaaga
    17701 ccatcggaat gatcgtccct gatgtcactg atttcttctt ctccaaactg atcgaaggtg
    17761 tagagagcta tttgaatcct ttgggctatg tgatcatgtt atgcaattct cgtcatagcc
    17821 aagaaaatga gatcaagtat ttacaagagc tttcccaccg ctcagtggac ggaatcttgc
    17881 tggccacacc aaatatttta cctgagaaat atgcattaga cagcggtttt tatcaaaaaa
    17941 tgccaatcat tttaatcgat cgcggattga atcgtcggga taacggacgg cttatcgtaa
    18001 aggaatatga gggcgcctat caagctgttt cgctgtttat tgaaaatggc catacgaaga
    18061 tcggcatgct gaaggaaacc actggatact atcaattaga agaacgtttc aacggatatc
    18121 gtcatgcgct gaaagatcat ggcctgcctt ttaatgggaa atttgttgaa catggagagt
    18181 tgactgtcca aggtggatat actgcatcaa aaaaattact caaacataaa gatatcacgg
    18241 ctattttttg cgggaatgat gccatggcga tcggatgcta tcaggcgatc gatgaattgg
    18301 gcaaaaaaat cccagaagat atctcagtca taggattcga tggattaaag ctgtcagaat
    18361 acatgattcc gcaattgacg acagtgcagc agccaagttt tgatattgga ttttatgctg
    18421 cacgtttcct gatcgatacg atcgaatttc ctcaaagaaa agttccgaac aaagtgttcg
    18481 aaacaaaatt aattattcgt gagagcgtaa aagtattgac aaagggctaa ctcggcggta
    18541 tattgcttat gtaagcattt acaaaggcta gttagtgctt tttttatttt tagtaaacca
    18601 gtttactaaa gtggtttacc tttcctaaaa ataatgtata ataccgaatg aagacaagga
    18661 ggatcaggca atgcgaatgg tggatctgat tgaaaaaaag agggatggaa acgagctgtc
    18721 gaaagaggaa atcgagtaca tcgtcacgaa ctatacgaat ggaaagattc cagattatca
    18781 agtcagtgca ttattgatgg caatttttta tcaagacatg acaaatgaag aaatcacgaa
    18841 cttaacattg gcgattgcta attctggtga tgtcatcgat ttgagttctc ttgaaggaat
    18901 aaaggtagat aagcattcga caggtggcgt tggtgacacg accacattga ttttagcccc
    18961 cctagtagct agtgtcggag tgacagtggc taaaatgtct ggacgcggtc taggttacac
    19021 aggtggtaca ctagacaagc tagaagcaat tcccggattt cagatcgagc tttcggatga
    19081 agaatttgta cgcattgtta atgaaagtaa agtagcggta attggtcaat caggaaactt
    19141 ggcaccggcg gacaaaaaat tgtatgcctt gcgtgacgtc acagcgaccg ttgattctct
    19201 tccgctgatt gccagttcga ttatgagtaa aaaaatcgcg gcaggagcag atgcaatcgt
    19261 tttggatgtg accactggag acggtgcttt catgaagaac atcgaagatg caagacgtct
    19321 ggcaaagacc atgacgagca ttgggaaact ggctaaccga gaaacagtgg cggttatctc
    19381 agatatgtca gaacctcttg gcgaagctat cggaaatagt ctagaagttg tcgaggcaat
    19441 cgaaacatta caaggaaatg gtccagaaga tctagtagaa atgtgctacg ccttaggaag
    19501 ccaaatggtt gttcttgctg gcaaagctaa gacaaccgat gaagcaagaa cgttgcttca
    19561 agaagctctg gaatcaggta aagcgttagc aaaattcaaa gaaatgatcc aaaatcaagg
    19621 gggagaccca gcgatcgtgg aacatccaga acgaattttg actgcacgct acacaatgga
    19681 gcttcctgct aaacaaagcg gagttgtgtc aaaaatcgtg gcaaatgaat taggaattgc
    19741 tgcaatgatg ctaggtgctg ggcgtaaaac caaagaagaa gatattgatc acgctgttgg
    19801 attgaaactg cataaaaaaa ttggtgacac tgtgacaaaa ggtgagtcac tattgacgat
    19861 ctatagtaac gacgaagaaa tcacttctgt cattgaatta ttatataaaa atattgaaat
    19921 tggtgaatcg gcaatgaagc caacgttgat tcatgacatc atcacagaat aaaggaaaaa
    19981 agcggacttt accgttttaa agaaaagggg aaaagaaatg gaactaaacc gaatgatcga
    20041 ccatactatt ttgaaagcag acgcatctaa agaagctgtg atgcaaatta tcgaagaagc
    20101 aaaaaaatac catttttatt ctgtctgcat caatccagca tgggttgcgc tagcgaaaga
    20161 acaattacaa ggtgagccag tagccgtatg tacagtaatc gggtttcctc taggagcaaa
    20221 cacacccgag acaaaagcat ttgaaacaac agatgccatc aataacggtg cagatgaagt
    20281 cgatatggtc atcaatatcg gtgctttgaa atcaaaggat gatcaacaag tccaaaaaga
    20341 tatcgaagca gttgttgaag cagcaaaaga tcgtgcgctt gtcaaagtta ttattgaaac
    20401 atcgttatta gatagagaag aaatcatccg agcttgcgaa atagcaaaag ctgcaggcgc
    20461 tgattttgtc aaaacatcaa ctggtttctc aacaggcgga gcaaaggtag aggatgtccg
    20521 tttgatgcgt gaaactgtag gaccagaaat gggtgtcaaa gcttccggcg gtatccataa
    20581 cgaagaagaa gcaatggcaa tgatcgaagc tggtgctaca agaatcggaa caagcgcagg
    20641 agtagcaatc gtatctggtt caactggtga aggctattaa ttttttcaaa ggagaagctc
    20701 atggcagaac tcaaacaaga atggatcaac acagctattg aagcgttaga taaagcctat
    20761 gttccttatt cccattttcc ggtaggcgct tgtctcgtaa cggaatcagg caagatttat
    20821 caaggcatca acattgagaa tgcatcattt ggattaacga attgtgccga gcgcacagct
    20881 tttttcaaag cagtatctga aggagaaaaa acatttacgc atctagtcgt agcggggcac
    20941 acccccgatc ctatctctcc ttgtggtgca tgtcgacaag taatggcaga attctgttcc
    21001 cctgatatgc cggttacgct agtaggagac aatggtgtga cgaaggcaac gaccgttcgt
    21061 gcactgctgc cttatgcatt tactgaaaaa gatctataac tcatgttcat tcagtcagct
    21121 gactgttgaa aaataaaaca attattttag gaggctttac agaaatgaaa aaagcaaaat
    21181 tatttggttt aggtgctgtc gcactagcag cagggttatt tttaggtgca tgcggcaaca
    21241 acggctctac tgattcatcc ggtggcaaaa gctcatcaga cacaacaact gcagcattga
    21301 tcacagacac aggtggtgta gacgaccgtt cattcaacca gtcagcttgg gaaggactag
    21361 aaaaatgggg gaaagatcaa ggactttcac gaggaaatga tggcttccaa tatttccaat
    21421 cttcaaatga atcagattac atcccaaata ttgaccaagc attgaatgct ggattcaaaa
    21481 cgatttttgg tattggttat aaactaaaac cagcgatcca agaacaagca acaaacaata
    21541 caggtacgaa ctttgttatt atcgatgatg tcatcgatgg attagataac gttgtatctg
    21601 caactttcaa agacaacgaa gcttcttatc ttgcaggtgt tgcagcagct tatacgactg
    21661 aaacaaacgt agtcggcttt atcggcggtg taaaaggtga agtaatcgat cgttttgatg
    21721 ctggattcaa agccggtgtt gatgcaggtg cgaaagaatt aggaaaagaa atcaaagtat
    21781 tgaatcaata tgctggtgac ttctctgctc cagataaagg acgttcaatt gctcaaggta
    21841 tgtatgcaca aaacgcagat atcatcttcc atgcttcagg cggtacaggt aacggtgtat
    21901 tccaagaagc aaaatcattg aacgaatcag gcgacaaaaa agtttgggtt atcggtgttg
    21961 accgcgacca atcagacgaa ggcgaataca cattaaacgg cgaaaagaaa aacttcacat
    22021 tgacatctac actgaaagca gttggaacag tagtagaaga tttggcacaa aaatcagcgg
    22081 acggtaaatt ccctggcggc gaacacactg tttatggatt gaaagaagac ggtgttggtc
    22141 taacagaagg acaactttct gacgaagcga aaaaagcagt tgatgaagca aaagaaaaaa
    22201 tcatttcagg tgacgtaaaa gttccagaaa ctcctgaaga aaactaaaac catttctata
    22261 gccttgttag acacatgttt gacaaggcta tggtttgatt agaacaagaa ttgttcattt
    22321 atccaagctg ccttgtgaca gtttggataa gggaacaact ccctgaagta atgtttgttt
    22381 caaaagaaga acgctcacta agcgtgaatc ttagtcaatc gttgccctag atagaaatcg
    22441 ttaggatgtg aaaaaaaggt ggttcaagaa aatattgtca tagaaatgca gaatattacc
    22501 aaagaatttg gaacattcaa ggcaaatgac aacatcaatt tacaagtaaa ggcaggagaa
    22561 attcatgcgt tgcttggaga aaacggtgct ggcaaatcta cattgatgaa cgtgctttcc
    22621 ggattattag agccgacatc agggaaaatt ttgatgcgtg ggaaagaagt aaagatcaca
    22681 agtccgacca aagccaacca attagggatt gggatggtcc atcagcactt tatgcttgtt
    22741 gatgccttta ctgtaacaga aaacatcgtg ttgggaagcg agcctagtcg cgcagggatg
    22801 cttgaccata aaaaagcacg aaaagagatc caaaaagttt ctgaacagta tggattgtca
    22861 gtcaacccag atgcttacgt tcgtgatatt tcagttggaa tggaacaacg ggtagaaatt
    22921 ttaaaaacac tttaccgagg agcagatgta ctgatttttg atgagccgac agctgtattg
    22981 acccctcaag aaattgatga attaatcgtg atcatgaagg aattagtcaa agaaggcaag
    23041 tcgatcattt tgattacgca taagttagat gaaatcaaag cagtagctga ccgctgtaca
    23101 gttatccgcc gtggaaaagg aatcggtaca gtcaacgtta aagacgtaac ctcacaacaa
    23161 ttagccgata tgatggttgg aagaacggtt tcattcaaaa cgatgaaaaa agaagcgaag
    23221 cctcaagaag tcgttttgtc tattgaaaat ctagtggtga aagaaaatcg tggattagaa
    23281 gccgtgaaaa acttgaactt agaggttcgt gctggcgaag tacttggtat cgctggaatc
    23341 gatggaaacg ggcagtcgga gttgatccaa gctttgactg gtttgcgaaa ggcagaaagc
    23401 ggacatatca agctaaaagg ggaagacatc actaataaaa aaccccgaaa aatcactgaa
    23461 catggtgtag gacacgtgcc agaagaccgt cataaatacg ggttagtcct agatatgaca
    23521 ttatctgaaa atatcgcact acaaacctat catcaaaaac cttacagtaa aaacggcatg
    23581 ctgaattatt cagtaatgaa tgaacatgcc agagaattga tcgaagaata tgatgttcga
    23641 acaacgaatg aacttgttcc cgcaaaagct ttatcaggcg gaaatcagca aaaagcaatt
    23701 atcgctcgtg aagtcgaccg agatcctgat ctgttgattg ttgcaaatcc aactcgtgga
    23761 ctggatgtag gtgcgatcga atttattcat aaacgtctga tcgaacaaag ggacaaatac
    23821 aaagcagtgt tactgatcag tttcgaatta gaagaaattt taaatgtttc ggatcgtatt
    23881 gctgttatcc atgaaggaga aattgtcggt atcgttgatc cgaaagaaac atctgaaaat
    23941 gaattaggtc tattgatggc gggctatacc ttagaagaag cacgaaaaga gttagaaaag
    24001 ggggctggcg cacatgaatg atcgcagcga aaaattccgt aatttattag tgcctgtact
    24061 ttcagttgtt ttaggattga tcttaggcgc gatattgatg gctgcttttg gttttcagcc
    24121 catccaaggt tattcttcta tgctagatgc agcccttggt tcgcaaagag ggataggtga
    24181 aacgttaagg caagcaacgc cgttgatttt tacagcatta ggtttttctg tagctaactc
    24241 agctgggttt ttcaatattg ggctttctgg acaagcactt tgcgggtgga tcgtcagtgt
    24301 ctgggtggca cttgcgtttc cagatctgcc aaaagttgtt ttgcttccgt tatgcgtgat
    24361 cttaggtgcc ttagctggtg cgatggcagc ggctgttcca ggtttacttc gagcattttt
    24421 tggaacaagt gaagtgatcg taacaatcat gatgaactat atcttattat acgtaagcac
    24481 atttgcttta catgaagtga tgcctgaaag ctatcgctca agtttggact cttccaatat
    24541 gatcagtcaa aatgcctcac tgcggttacc atggttaaca caagcttttg gcggctctcg
    24601 cgtaaactca ggattatttt tagcaatcat cgcgcttatt ttcgtttggc tgctgatgaa
    24661 gaaaacgact ttaggatttg aaatccgttc tgttggattg aatccttttg cttctgagta
    24721 tgcaggaatg agtagtaaac gtacgatcgt tttgtcgatg gtcatttctg gcgctttggc
    24781 agggcttggc ggtgtggttg aaggattagg aacgtatcaa aacttctttg ttcaaacgac
    24841 ttcgctatct attggatttg acggaatggc tgtttccttg cttggttctg gatcagcaat
    24901 cggtattctt ttatcagcga tcttgttcag tattttgaag atcggtggac ttggcatgca
    24961 gacaggagca ggtgttccgt ttgagatcgt caatgtatcg attgctttga ttatcttctt
    25021 tgtcgggatc aacttcctga ttcgtttcgt catggctaaa gtcttccaag ggaaaaaaca
    25081 agaagagatc gttgcgacaa tagaatcgaa accaacgaat caaaaccatg aagggggcga
    25141 actataatgt cgactatcga attaattgcc ttgatcctta cttctacact ggtttattca
    25201 acaccgctaa ttctaacgtc actaggcgga actttttctg aacgaagcgg gatcgtaaac
    25261 gtagggctag agggaatcat ggtcatgggt gcttttagtt ccatcgtttt caacttgacg
    25321 atgagtccat cacttggttc tatgacacca tggatcgcta ttttagtggg tggcgtcgta
    25381 ggtatcctat tttctctgat tcacgcagta gcaacagtca gtttgcgtgc tgaccatatt
    25441 atcagcggga cagtaattaa tctgatggca cctgctttag gtgttttctt gatcaaagca
    25501 tggtatggta aaggtcaaac tgataacatc acgcagaatc tggggtattt ttctttccca
    25561 ggattagcag atattccggt cattggacca attttcttta aaaatacatt cttacctgct
    25621 tattgtgcta ttttgattgc tgtcctagct tgggtgatca tcttcaagac gaaattcggc
    25681 ttgcgcctcc gttcagttgg tgaaaatcct caagctgccg acacattagg tatcaatgta
    25741 tatgctatgc gttattcagg cgtactgatt tctggcttgt taggcggtat gggcggtgct
    25801 gtttttgctc agtcgatttc cggtaacttt tcagctggta cgatcgttgg acaagggttc
    25861 atctccatgg ctgctatgat ctttggtaag tggaatcctt taggggcaat gggtgcctca
    25921 ttgttctttg gttttgctca aagcttgagt atcattggcg cacaattacc agttatttct
    25981 tcgattccgc cagtattgct gcaaattgct ccatatttgc tgacaatcat tgtgctggtt
    26041 gttttcttag ggaaagcttc tggtccaaaa gcaaacggga agaactatat caaatcaaaa
    26101 taatctttta gcgaataaaa agggtgggtt acgggaagtg tttttcgtct cgcccttttt
    26161 gttttaaagg caataaaaat aaaaagaaag aaggaataat catgtttaaa cgcgtacatc
    26221 tgatcgtcat ggattcagta ggaattggag aagcaccaga tgccgataag ttcggtgatg
    26281 ttggttctga tacattagga cacattgcga aagaagcagg attgaacgtt ccacatttag
    26341 agcagcttgg tttaggaacg atccgtcctc ttgaaggtgt ggaatcagtg aaagatcatc
    26401 aaggctatgc gacgaaatta gaagaagttt ctgtagggaa agatacgatg acaggccact
    26461 gggaaatcat gggattgaat atcaaaactc catttcgtgt gtttcctaat ggattccctg
    26521 atgatctgat caaacaaatc gaagaatttt ctggccgtaa agtcgtatgt aacaaacctt
    26581 atagcggaac agctgttatt gacgattacg gtgaacacca aatgaaaaca ggcgatttaa
    26641 ttgtatatac ttctgccgat cctgtccttc agatcgcggc acatgaagaa atcattcctt
    26701 tagaagaatt atatcggatt tgccaattcg ttcgtgatat aacaaaagat gatccgtata
    26761 tgattggccg aatcattgca cgtccgtacg tgggtgaacc aggagacttc acacgaacaa
    26821 gcaatcgcca tgattatgcg ttgaatccat tcggacacac agtccttgat tctttgaaag
    26881 aagcaggaaa ggacgtgatt gctgttggaa agatcaacga tattttcaac ggacaaggaa
    26941 tcactgaatc tgtccgcacg aaaagcaata tggatggcgt tgaccagctg ctgaaagtta
    27001 tgaaaaacga tttttcagga atcagcttta ccaatttagt agattttgat gcattatacg
    27061 gtcatcgtcg ggatgtagtc ggctatgcgc atgcgattga agaatttgac ttacgaattc
    27121 cagaattgct tgaagcgatg gaagaagacg acttgctaat gattacagcc gatcacggaa
    27181 acgatccgac gtttactggt accgatcata cgcgagaata tgtaccgtta ttggtttaca
    27241 gtaaaaaaat gaaagaacaa ggggcattgc ctcaaggata ttattcagat atttcagcaa
    27301 cgattgctga gaactttagt gtttcttcga ctgaaaatgg cgaaagcttc ttgaaattat
    27361 taaaataatc tttttaaata ggagaaagca aatgaccaag ttaagcgaga tgttacaaga
    27421 aacgacccag tttatccttg ataaaggagt aaaagaaatt gattttggtt tgattctagg
    27481 ttcaggatta ggtgaattag cagatgaaat cgaagatgct gtaatgattt cttatgatga
    27541 gatcccgttc tttcctgttt cgacagtagt tgggcatgca ggaaagctag tttatgggtc
    27601 tctggcaggc aagaaagtgt tagccatgca agggagattc cactactatg aaggtcattc
    27661 catgcaaaca gtgacctatc cagtacgtgt gatggccgga ctaggggccc attcagtcat
    27721 tgtgactaat gcatgtggtg gtgtcaacga atcatttgta ccaggagatt tgatgctgat
    27781 taccgatcat atcaatttta ctggtgaaaa tcctttgatc ggtccgaatg aagatgcaat
    27841 gggccctaga tttccagata tgagtcaagc ttatacgcct gactatcaag caacagcaaa
    27901 agcagcagca gaaaaactag gtttggatct taaagaaggc gtatatatgg gctattctgg
    27961 accaacttat gaaacgcctg cggagatccg aatgtctcgg acaataggag ccgatgcagt
    28021 agggatgtcg actgttccag aagtcatcgt agcagctcac agcggattaa aagtgttagg
    28081 aatctcttgt attaccaact tagcagcagg tatgcaagcg aatctgagcc atgaagaagt
    28141 tgtagaaaca actcagagag tcaaacaatc ttttaaagca ttggttaaag aaacattgac
    28201 attattgtaa taggaggaac aataaaatga gtgtgcatat tgaagcgaaa gaaggcgaga
    28261 tcgccgataa gattttactt ccgggagatc ctttgcgagc taaatatatt gcggagactt
    28321 ttttggaaga tccagtttgt tacaatcagg tacgcggtat gttaggcttt accggcaaat
    28381 ataaaggaga acgtatttct gttcaaggaa caggaatggg aatgccatca gccaccatct
    28441 atgcccatga attgatccag tcgtatggtg taaagaaatt gattcgagta ggtacatgcg
    28501 gtgcattgtc aaaagacgtc catgttcgtg atttagtact agcacaaggt gcagcgacca
    28561 gttcttcgat gatcgaaaaa aatttccaag cctttcattt tcctccaatc agtgatttca
    28621 atctattact gaaagcatat gagatagcta aagaaaaggg ctatacggtt catgtaggaa
    28681 atgttctttc ggaagattct ttctacaaag atgatttgac agagacgttc caattagctg
    28741 aacttggcgt tttaggagtg gaaatggaag cagcggctct ttattactta ggcgcgaaat
    28801 accatgtgca gacattaagt ttgatgaccg ttagcgacca tttgatcacc ggtgaagaaa
    28861 caacagctgc agaacgtcaa tctacgttca atgaaatgat cgaagtagga ttggaaacag
    28921 cgatagcaga ataagaagga aatgaaaaag ctgccctgca ttaagtaata agaacatcca
    28981 agcaaaaata gctcctcatg tttttgactt ggctggactt attattgcaa atgtaagctt
    29041 ttgaacaccg tttaccaaga ggttgtgaat ctgccgttta actctgagtc acaaggaaca
    29101 atttcaaaaa acagcttttc atgtttgatg aatattgtga cttgtgaccg agaagttggc
    29161 aggagaaaac cgtttatacg aaaaaaggga ctgtgacaaa tcgtgtcaca gtcccttttt
    29221 tatagtgttt tttataggta gtatttttta gcaactgtta agtcatcatt taattcataa
    29281 acaagtggtt gacctgttgg aatttcaaga tccatgatgt cttcgtcaga gataccttcg
    29341 atatgtttag ctagggcgcg taatgagtta ccatgtgcag ctactaaaac agttttgttg
    29401 tctagtaatg ctggagcaat ttcatcttgc cagaatggca aagcgcgttc taatgtcact
    29461 ttcaagtttt ctccaccagg aatatcgcgt ttgtctaaca ttgcgtaacg acggtcattt
    29521 gctgcagaac cttcgtcagt ttcttccatt aatggaggaa gagtatcgta agaacgacgc
    29581 cagatgtgca cttgttcgtc gccgtatttt tcagctgttt cttttttgtt caaaccttgt
    29641 aatttaccat agtgacgttc gtttaagcgc catgatttga tttgtggtac ccaaagttga
    29701 tctgaatatt ctaagatcaa gttacatgtt ttgattgcac gagttaaaac agaagtgtaa
    29761 gcaacatcaa attcgattcc agcttctttg attttgcgtc cgccttcttt tgcttcttcg
    29821 attccttctg gtgctagatt tacatcagcc caaccagtaa attggttcaa tgcattccat
    29881 tcactaagac cgtgacgaga aaaaactaat tttggcatgt ttagctctcc ttttaagata
    29941 ggtttatatt tctgaaattt tcagttctgt tttattgtac aattttttgg aagagatttc
    30001 catcgaaaaa ttagataaga ttctatgaga aaacaagtag agaatataaa aaaacgaaca
    30061 gtgcccacgc aactgttcgt ttttaggtat tagatgcata attgctcgat ttctttttca
    30121 ggtaaattga tatatttata acatgttcca actgaaacac cgcattttga agaaataaat
    30181 tggatcgttt cttttttttc ataatagaga cgtcggattt ttttgatcgt ttttgcatcg
    30241 atttttggtc ttccgccgat ttttcctttt ttacgagctt catctaatcc aactaatgta
    30301 cgttctttga tcaatgcgca ctccatgttt gctaggtttt ccattaattg gaaatagatt
    30361 tttcccattt cgcgcgtatc gattccttca tcaagactaa tcaagtgcag cccgcgatct
    30421 ttgaataatt gcgtgagttc tgttaattgc cgcgtagatt tgcctaatcg atgaagttgg
    30481 caaatgacta gtgcatcgcc ttgttgcatg tcagcgatca ctgactccaa atgaacagca
    30541 gtagaatcat tttgttccac ttcatatgtc tcatggaaaa tttcttgaca accaaagtca
    30601 gataattgat caacctgagt tgttaaatca acgtcattta tggttgtgcg tgcataacca
    30661 ataattttca tggtgattcc tttctagcag tgattttcta actggatata acagtaaaaa
    30721 tgtagcataa agtacgtcaa aaagatagtt ttttcgcaaa ttttccgctg tcttttttta
    30781 ttggatgatc aagcagtttt tctttattta cgacggattg cgtcaagaat ctcaacgcct
    30841 ttttctgagc cgacaactac tttagaaacc atgtttagga aaagcccatg ttctactacc
    30901 ccaacaagtt catctaaata ggtagctaag ctttctggct gttcgatgac ttctagatgc
    30961 agatcaatga tgtaatgacc accatctgtg gttaagactt ctccgttttc gtctgtacgc
    31021 aagacgggtt ggaatctttt ttcttcaaaa atatgaagta gctgttggga gccataaggg
    31081 atcacttcaa ctgggagtgg gaacttccca agtttatcga ctaatttaga agaatcaacg
    31141 atccaaatcg tttcttttga gtacgtagcg actattttct cgaaaagcaa agcagcgccg
    31201 ccgcctttga tcccttgaaa atcagcactg atttcatcag caccgtctat tgtcaaatcg
    31261 actacgggga cttcatcaat actctttaac ggtattccta atttttccgc ttgttctttt
    31321 gtcgcatttg aagtagtaac tcctgtgata cgtaaacctt cttcacgcat acggcgacca
    31381 atctcttcta ccatataata ggcggttgaa ccggtcccga gacctacgat catcccgtcc
    31441 tttacgaatt tcgcggcttc aatgccagct aattgtttta aattcatctt tgcgtccacc
    31501 tttttttagt ttgagcatcg tgctttcctc actattttag cgtgatttac ggtaaaaaga
    31561 taccattctt ttttattcca ataaagcttt ttcaaaaaaa attgcatctc ttgttgacat
    31621 gttttttcat tcgtgatatt ctaacaaagg tgctttatct acaggtttct gtgaagaaac
    31681 gtgctgactg tttatcaaag catatttgat aaggatgcag acattgcatt tattttttat
    31741 cacaaaaaaa gaaccacctg gatgtgtgga actagacgca aatcgtggga aggaggaaac
    31801 aaggaatgcc tacaattaac caattagtac gtaaacctcg taaatcaaag gtggaaaaat
    31861 ctaattcacc tgctttgaac aaaggatata acagctttaa gaaaactcaa actaacgtaa
    31921 actcacctca aaaacgtggg gtagcaactc gtgtgggtac aatgacacct aaaaaaccga
    31981 actcagcttt acgtaaatat gcacgtgttc gtttgtcaaa cttaattgaa gttactgctt
    32041 atatcccagg gattggtcat aacttacaag aacacagtgt tgtattatta cgcggtggtc
    32101 gtgtaaaaga cttaccaggg gtacgttacc atatcgttcg cggtgcttta gatactgctg
    32161 gtgttaacga ccgtaaacaa agccgttcta aatacggaac taaaaaacct aaagcttaat
    32221 tttaattatt aaacaaatga aacttgcttc tactatatag aagtaattac tatgaacaat
    32281 atttcggaag gaggagttac ggatgccacg taaaggtcct gttgcaaaac gtgatgtttt
    32341 accagatcct atttataact caaaattagt aactcgcttg atcaaccgtg taatggttga
    32401 cggaaaacgc gggattgcag ctaacattat ctataatgcc tttgacttga tcaaagaatc
    32461 tacaggtaac gatccattag aagtgtttga acaagcaatg aaaaacgttc aacctgttct
    32521 tgaagttaaa gcacgtcgtg ttgggggttc aaactaccaa gtaccagttg aagttcgccc
    32581 tgaacgccgc gttactctag gtcttcgttg ggtagtaaac tacgctcgct tgcgcggtga
    32641 acacacaatg gaagaacgct tagcaaaaga aatcatggat gctgcaaaca acacaggtgc
    32701 ttctgttaaa aaacgtgaag acactcataa aatggctgat gccaaccgtg cgtttgctca
    32761 ctatcgctgg taagattctc tctgtttggt cggcttttat aagtcgacct cagatagtgt
    32821 ccttattttt agcaactaga tagtaaagag aggagaaaca aggagaatga ccagagaatt
    32881 ttctttacaa aacactcgta atatcggtat catggcccac atcgatgctg gtaaaacgac
    32941 tgctactgaa cgtatcttgt actatactgg taaaatccat aaaattggtg aaactcatga
    33001 aggagcttca caaatggact ggatggaaca agaacaagaa cgtggtatta cgatcacttc
    33061 tgctgctact acagcacaat ggaagggtca ccgaatcaac atcatcgaca caccaggaca
    33121 cgtagacttc acagttgaag ttgaacgttc attgcgtgta cttgatggtg ctgtaactgt
    33181 tcttgatgca caatcaggtg ttgaacctca aactgaaaca gtttggcgtc aagcaacaac
    33241 ttatggtgta cctcgtatcg tatttgcaaa caaaatggac aagatcggtg ctgacttctt
    33301 atattcagta tcaactttac atgaccgttt gcaagcaaac gctcacccaa tccaattacc
    33361 aattggtgcg gaagacgact tcacaggaat tatcgacctt gtaacaatga aggctgaaat
    33421 gtatacaaac gatcttggaa cagagatcga agaaacagaa attcctgaag aataccgcga
    33481 attagctgaa gaatggcgcg aaaaattggt tgaagctgta gctgaaactg atgaagaact
    33541 tactttgaaa tacttagaag gtgaagaaat cactgaagct gagttaaaag aaggtatccg
    33601 ccgcgcaaca gttaacgtag aattctaccc agtactatgt ggttcagcat ttaagaacaa
    33661 aggggttcaa ttactattgg acgctgttct tgattacttg ccatcaccgt tagatatccc
    33721 agctatcaaa gggattgacc ctaaaactga tgaagaagta gaacgtcctg cagacgacag
    33781 cgctcctttc tcagctttag catttaaagt tatgactgac ccattcgtag gtcgtttaac
    33841 tttcttccgt gtgtactctg gtgttttaca atcaggttca tacgtacaaa acgcaacaaa
    33901 aggcaaacgt gaacgtgttg gtcgtatcct acagatgcat gccaactctc gttcagaaat
    33961 ctctgaagtt tacgctggag atatcgctgc tgctgttggt ttgaaagaca caactactgg
    34021 ggacacacta tgtgatgaaa agagtctagt tattcttgaa tccatggaat tcccagaacc
    34081 agttatccaa gtcgctattg aacctaaatc aaaagctgac caagataaaa tgggtgtggc
    34141 tttacaaaaa ctatctgaag aagatcctac tttccgtgct gaaacaaacg tggaaacagg
    34201 cgaaacaatc atcgctggta tgggtgaact tcacttagat atcatcgttg accgtatgcg
    34261 tcgtgaattc aaagttgaag ctaacgtagg tgcgcctcaa gtatcttacc gtgaaacttt
    34321 ccgtgctggc actcaagccg aaggtaagtt tgtacgtcag tctggtggta aaggtcaata
    34381 cggtcacgta tggattgaat ttacaccaaa tgaagaaggt gctggtttcg aatttgaaaa
    34441 tgcgatcgtc ggtggggttg ttcctcgtga atacattcca gctgttgaaa ctggtttgaa
    34501 agatgctatg gaaaatggtg tcttagctgg ctatccatta gtagatatca aagctaaact
    34561 ttacgatggt tcataccatg atgtcgattc aaatgaaact gccttccgtg ttgctgcttc
    34621 tatggcatta cgtgcagcag ctaagaaagc taatccagta atccttgaac caatcatggc
    34681 tgtagaagtt gttatccctg aagattattt aggtgatgtt atgggtcacg taactgctcg
    34741 tcgtggacgt gttgaaggta tggaagctcg cgctggcggc caacaagttg ttcgtgcgat
    34801 ggttccactt gctgaaatgt ttggttatgc tacaactctt cgttctgcta ctcaaggtcg
    34861 tggtacattt acaatgacat ttgaccacta cgaagatgta ccaaaatcag tacaagaaga
    34921 aatcattaag aaaaacggcg gaaaagctga ataatttctt gaaaccggat agtcctgcaa
    34981 agagattatc caaagagact atatttttaa tatagtttcg tgtaaaataa ttatcgatga
    35041 tatgggcatg tggtgtacct tgtgtacatc atatacctat cagacaaata aaaaatatat
    35101 tgtattttta ggaggacatt ttaaaatggc aaaagaaaaa tttgaccgtt ctaaaccaca
    35161 cgttaacatt ggtactatcg gacacgttga ccatggtaaa actacattaa cagctgcaat
    35221 cacaactgta ctatctaaga aaaacggcgg ccaagctatg gcttacgatc aaatcgatgg
    35281 tgctccagaa gaacgcgaac gcggaatcac aatctcaaca gctcacgttg agtatgaaac
    35341 agatacacgc cactacgctc acgttgactg cccaggacac gcggattacg ttaaaaacat
    35401 gatcactggt gctgctcaaa tggacggagc tatcttggta gtttctgctg ctgacggccc
    35461 aatgcctcaa actcgtgaac acatcctatt gtctcgtcaa gttggtgttc cttacatcgt
    35521 tgtattcttg aacaaagtag acatggttga tgacgaagaa ttactagaat tagttgaaat
    35581 ggaagttcgt gacctattaa cagaatacga attccctggt gacgatgttc ctgtagttgc
    35641 tggatcagct ttgaaagctc tagaaggcga cgcttcatac gaagaaaaaa ttcttgaatt
    35701 aatggctgca gttgacgaat acatcccaac tccagaacgt gacaacgaca aaccattcat
    35761 gatgccagtt gaagacgtgt tctcaattac tggacgtggt actgttgcta caggtcgtgt
    35821 tgaacgtgga caagttcgcg ttggtgacga agttgaagtt gttggtattg ctgaagaaac
    35881 ttcaaaaaca acagttactg gtgttgaaat gttccgtaaa ttgttagact acgctgaagc
    35941 tggagacaac attggtgctt tactacgtgg tgttgcacgt gaagacatcc aacgtggaca
    36001 agttttagct aaaccaggta caatcacacc tcatacaaaa ttctctgcag aagtatacgt
    36061 gttgacaaaa gaagaaggtg gacgtcatac tccattcttc actaactacc gtccacaatt
    36121 ctacttccgt acaactgacg taacaggtgt tgttgaatta ccagaaggaa ctgaaatggt
    36181 tatgcctggc gacaacgtaa caatggaagt tgaattaatc cacccaatcg ctatcgaaaa
    36241 cggtactaaa ttctcaatcc gtgaaggcgg acgtacagtt ggtgccggtg tcgttacaga
    36301 aatcaaagct taattcactt aagttttaaa gtaaagcaac atttattcgg acgtaatcgc
    36361 tcggaacgaa ggtcttagga tctttgttcc gagctttttt atttattgca ggatcaggaa
    36421 aaaagacagg agtattataa atagaagaaa cacgaaataa aagagtcagt attttctaaa
    36481 tgaaacttta tgaaaacaaa atgtttttta caagaaaaag ccttgctttg tgagtttata
    36541 tatattataa tgacaaaggt gcgtttactt tagtgaattg cacagacgta atcagaatga
    36601 tttcgtcggc taagaaacga gaggttgcga cacgcccgga cgctttgcca cgaacgagcg
    36661 tgacggaaat tttcgtggag ctatgtctac ttttcaaaat aggcgaagga gggaacaaga
    36721 tggcaaaaca aaaaattcgt atccgtttaa aagcgtatga acaccgtatt ttagatcaat
    36781 cagcggataa aatcgtggaa actgcaaaaa gaactggagc tagcgtgtct ggtccaattc
    36841 cattaccaac tgaacgcagt ctttacacaa tcatccgtgc gactcataaa tacaaagatt
    36901 cacgcgaaca attcgaaatg cgtacacaca aacgtcttat cgacattgtg aacccaacac
    36961 caaaaactgt tgatgctttg atgaagctag acttaccatc tggtgtaaac atcgaaatca
    37021 aattataaaa ctaaaacaaa aataatggag gtgtactcat gaccaaagga atcttaggga
    37081 aaaaagtggg aatgacacaa atcttcactg aatctggtga attaattcca gtaactgttg
    37141 tagaagctac tccaaacgta gtactacaag tgaaaacaat ggaaacggat ggctacgaag
    37201 ctattcaagt tggttaccaa gatatgcgtg aagttttatc aaacaaacct gcgaaaggtc
    37261 atgttgcaaa agcaaacacg gctcctaagc gcttcattcg tgaattcaaa aatgttgagc
    37321 taggagaata tgaagtagga aaagaaatta cagtagatgt tttccaagca ggagacatta
    37381 ttgatgtaac aggtactacg aaaggtaaag gattccaagg ggttatcaaa cgccacggac
    37441 aaagccgcgg accaatggct cacggttctc gttaccatcg tcgtcctggg tcaatgggtc
    37501 cagttgcacc taaccgtgta tttaaaaaca aacgtcttgc tggccgtatg ggtggcaacc
    37561 gcgtaactat ccaaaacttg gaagttgtac gtgtcgatgc agaaagaaat gttatcttga
    37621 tcaaaggtaa tgttcctgga gctaaaaaat ctttaattac tatcaaatca gctgtgaaag
    37681 ctaaataatt gtaggaagaa aggaggaact aaggaatgcc gaatgtagca ttattcaaac
    37741 aagatggaag ccaaaacggt gaaatcacac ttaacgaaga aatcttcgga attgaaccta
    37801 atgaaagtgt tgtttacgat gcaatcgtaa tgcaacgtgc gtcattaaga caaggaacac
    37861 acgcggttaa aaaccgtagc gctgttcgcg gcggcggacg taaaccatgg cgtcaaaaag
    37921 gaacaggtcg tgcacgtcaa ggatctatcc gctcaccaca atggcgtgga ggtggcgtag
    37981 tctttggacc aacaccacgt tcttacagct acaaacttcc taaaaaagtt cgtcgtttag
    38041 caatgaaatc tgtattgtca gaaaaagttg ctgaaaacaa cttggtagca atcgaaggct
    38101 tgaacttcga cgcaccaaaa acaaaagaat tcaaacaagt tcttgctaac ttatctatcg
    38161 actctaaagt attggttgtt ctagaaaaag gtaacgactt tgctgcttta tcagcacgta
    38221 acttaccaaa cgtttctgta gtaacttctg ataatgtgag tgtgctagac gttgtatcaa
    38281 atacaaaagt gttagcaaca caaactgctc ttactcaaat tgaggaggtg cttgcataat
    38341 gaacttacta gacgtaatca aacgcccagt gatcactgaa aaatcaatgc ttgccatgga
    38401 tgacaagaaa tacacttttg aagtggacac tcgcgcaaac aaaactttag taaaacaagc
    38461 ggtagaagct gcttttgacg ttaaagtcaa aaacgtgaac atcgtgaacg tacgtgcgaa
    38521 attcaaacgt atgggcaaat atgcaggata tacaaaaaaa cgtcgcaaag cgatcgttac
    38581 attgactgaa gattcaaaag aaatccaatt attcgaagct gctgaataag cactaataga
    38641 taactcatta aattaggagg gaaaaacgtg gcgattaaaa agtacaaacc tacctcaaat
    38701 ggtcgtcgta acatgacagg ttctgatttt gctgaaatca caacatcaac acctgaaaaa
    38761 tcattgttac aaccattgaa aaaccatgct ggacgtaaca acaacggccg catcactgta
    38821 cgtcatcaag gtggcggaca caaacgccaa tatcgcgtga tcgacttcaa acgtaacaaa
    38881 gataacgttg ttgcaactgt tcaaacgatc gaatatgatc caaaccgttc agctaacatc
    38941 gcgttagtac actatgaaga tggagtgaaa gcttacatct tagcacctaa aggactagaa
    39001 gtgggtatga aacttgtttc aggtccagaa gcagatatca aagtcggaaa tgctcttcca
    39061 ttagaaaaca ttccagttgg tactgttatc cacaacattg aaatgaaacc tggtaaaggt
    39121 ggacaattga tccgttctgc tggtacaagt gctcaagtac ttggtaaaga aggcaaatac
    39181 gtattgatcc gcttgaactc tggtgaagtt cgtatgatct tagctgcatg ccgtgctaca
    39241 atcggttctg taggtaacga acaacacgaa ttaatcaaca tcggtaaagc tggacgctct
    39301 cgttggatgc gtaaacgccc aactgtacgt ggtagcgtaa tgaaccctaa tgatcaccca
    39361 cacggtggtg gtgaaggtaa agctcctatc ggacgtaaag caccagtttc tccttggggt
    39421 caaccagctc ttggttacaa aactcgtaac aaaaaagcta aatcagacaa acttattgtt
    39481 cgtcgtcgca aaaataaata attactttga ccatgtgtcg cacattttga gaggaggttc
    39541 accatgggtc gtagtttaaa gaaaggacct tttgtcgatg agcatttaat gaaaaaagtt
    39601 gaagctcaag caggggttga aaagaaaaaa gttatcaaaa cttggtcacg ccgttcaaca
    39661 gttttcccaa gttttgttgg atataccatc gcagtttatg atggacgtaa acatgtacca
    39721 gtttacatcc aagaagacat ggtaggacac aaattaggtg aatttgcacc tacaagaact
    39781 tatcgtggac atgctgctga cgataagaaa acgaaacgct aatttgaggg gaggataaat
    39841 ccaaatggca gaacaaatca catcagctaa agcaactgca aaaacagttc gcacttcacc
    39901 tcgaaaagcc cgtttagtaa ttgaccttat cagaggtaaa agtgtcgcag atgcaatttc
    39961 aatcttgaaa ttcacaccaa acaaatctgc tggaatcatt gaaaaagtat tgatgtcagc
    40021 agttgcgaat gcagaaaata actttgactt agatgttgaa aacttggtag taagcgaagc
    40081 ttttgttaac gaaggaccta caatgaaacg tttccgtcct cgtgcaaaag gatcagcttc
    40141 tccaatcaac aaacgtacaa gtcatatcac ggtagtcgta tcagaaaaat aaggagggat
    40201 aatcagtggg tcaaaaagta catccaattg gaatgcgtgt aggcatcatc cgcgactggg
    40261 atgctaaatg gtatgctgaa aaagagtatg ctgagttctt acacgaagat ttaagaatcc
    40321 gtaaatttat ttcaactaaa cttgctgatg ctgctgtatc aacagttgaa atcgaacgcg
    40381 caacaggtcg cgtaaacatt tcaattcaca cagctaaacc aggtatggtt attggtaaag
    40441 gcggttctga agtcgaaagc ctaagaaaag aattaaacaa attaactggt aaaagaatcc
    40501 atatcaatat cgtggaaatc aaaaaaccag atctagatgc aaaattagta ggtgaaggaa
    40561 tcgcacgtca attagaaaac cgtgttgctt tccgtcgcgc tcaaaaacaa gccatccaac
    40621 gctcaatgcg tgctggcgct caagggatca aaactcaagt atctggtcgt ctaaacggag
    40681 cagatatcgc tcgttcagaa ggatactcag aaggaactgt tcctcttcat actttacgtg
    40741 cggacattga ttacgcatgg gaagaagcag atacaacata cggaaaacta ggagttaaag
    40801 tgtggatcta tcgtggagaa attcttccaa ctaaaaaaaa cactgagaaa ggagggaaat
    40861 aatcatgtta gtacctaaac gtgtaaaaca ccgtcgtgaa ttccgcggaa aaatgcgcgg
    40921 ggaagctaaa ggcggaaaag aagtagcatt cggtgaatac ggtttgcaag ctgttgattc
    40981 acattggatc acaaaccgcc aaatcgaagc tgctcgtatc gcaatgactc gttacatgaa
    41041 acgtggtggg aaagtatgga ttaaaatttt ccctcacaaa tcttatactg ccaaagcaat
    41101 tggggtacgt atgggttctg gtaaaggggc acctgaagga tgggttgcac cagtaaaacg
    41161 tggtaaaatc atgtttgaaa tcgcaggcgt tcctgaagaa gtagctcgtg aagcgttacg
    41221 tctagcttct cacaaattac caatgaaaac taagatcgta aaacgtgagg aaatgggtgg
    41281 tgaatcgaat gaaggttaaa gaaatcagag aattaaccac tgccgaaatg cttgatcaag
    41341 aaaaacaatt aaaagaagaa ttgtttaatc ttagattcca attagcaaca ggtcaattag
    41401 aaaacactgc acgtattaaa gaagtacgta aatcgattgc acgcatcaaa actgtgttgc
    41461 gtgaacaagc taagtaatta tggaaggagg ccattaagcg tatgactgaa ggaagaaatc
    41521 aacgcaaagt ttatcaaggt cgcgtggttt cagacaaaat ggataaaaca attaccgttg
    41581 ctgtcgaaac aaagaaaaac caccctatct atggcaaacg catgaactat tctaagaaat
    41641 acaaagtgca tgatgaaaac aacacagcaa aagttggcga catcgtgaga attatggaaa
    41701 ctcgtccatt atcagctaca aaacgtttcc gtttgttgga agtagtcgaa gaagcagtta
    41761 ttatctaata acacgagatt ttcctatata gattgaatgc aaaatctgaa aggaggatac
    41821 acaacgtgat ccaagcagaa agtcgtttaa aagtcgctga taactcaggc gcacgtgaaa
    41881 ttttgacgat caaagtcctt ggtggatctg gtcgtaaaac tgctaacatc ggtgatgtga
    41941 tcgttgctac ggttaaacaa gcaacgccag gtggggttgt caaaaaaggt gacgtcgtta
    42001 aagccgttat cgttcgaaca aaatcaggag ctcgtcgtac agacggttca tatattaaat
    42061 ttgatgaaaa tgctgcagtg attattcgtg atgataaaag ccctcgtgga acacgtatct
    42121 ttgggccagt tgcacgtgaa ctacgtgaaa acaacttcat gaagatcgtt tcactagcac
    42181 cagaagtttt ataatcttga gacacttatc aaaggaggtg cgaaacagtt atgtttgtta
    42241 aaaaaggcga taaagttaaa gttatcactg gaaaagacaa aaacaaagaa ggcgttgtac
    42301 tagcagcgtt cccgaaaaaa gataaagtta ttgtcgaagg tgtaaatgtc gttaaaaaac
    42361 accaaaaacc ttctcaagct gctcctcaag gtggaattgt tgagatggaa gcgccaattc
    42421 atgtttctaa cgtgatggtg gttgactcta ctggtgtagc tggccgagtt ggctacaaag
    42481 aagtagacgg caaaaaagtc cgtgtttcta aaaaaaccgg tgaagtttta gataaataaa
    42541 agtttaagga aggagggtct tactgaatga accgcctaaa agaaaaatat cttaaagaag
    42601 taactccatc attgatggaa aaatttaact atagctctgt tatgcaaaca cctaaagttg
    42661 aaaaaatcgt catcaacatg ggtgttggtg atgcggtatc aaacgctaaa aacttagata
    42721 aagcagttga agaattaact ttgatcactg gtcaaaaacc aatgattaca aaagctaaaa
    42781 aatcaatcgc tggattccgt ttacgtgaag gaatgccaat tggtgcaaaa gttacattgc
    42841 gcggagaaag aatgtacgaa ttcttagaca aattggtttc tgtttcacta ccacgtgtac
    42901 gtgacttcca tggtgtaagt aaaaaagcct ttgacggtcg tggaaactat actttaggta
    42961 ttaaagaaca attgatcttc ccagaagtag attatgattt agtagataaa gtacgtggta
    43021 tggacatcgt tattgtaaca acagcaaact ctgatgaaga atctcgtgag ttgttgacac
    43081 aattaggcat gccattccaa aaataattaa aggaggcgaa ctacgtggct aaaaaatcaa
    43141 tgattgctaa aaacaaacgt cctgcaaaat tttcaacaca agcttatact cgttgcgaac
    43201 gttgcggacg tccacattca gtttatcgta agttccatct ttgccgtatt tgcttccgcg
    43261 aacttgccta taaaggtcaa attcccggcg tgaagaaagc tagctggtaa aaagtaaatt
    43321 cgcataagaa ggaggtaaaa gatcaatggt catgacagat ccaattgcag attttctaac
    43381 tcgtatccgt aacgcaaaca tggttaaaca tgagtcttta gaagtacctg catcaaaaat
    43441 taaacgtgac atcgctgaaa tcttgaaacg tgaaggtttc gttcgtgatg ttgaatacat
    43501 cgaagatgac aaacaaggcg tgatccgtgt tttcctaaaa tttggtaaaa acggcgaacg
    43561 tgttatcaca aacttaaaac gtatctctaa accaggtttg cgtgcttatg tcaaagctga
    43621 cgaagtacca aaagtattga acggtttagg tatcgcaatc atttcgactt ctgaaggtgt
    43681 tgtcactgat aaagaagctc gtgaaaaaaa tatcggcggc gaagttatcg cttacgtatg
    43741 gtaatcaagt agaatctaca aggaggtgtc tttaagtgag ccgtatcgga aataaagtcg
    43801 ttgttatccc tgaaggagta acagttactc aagacggaaa caacgttaca gttaaaggac
    43861 caaaaggtga attgactcgt actttttcag cagatatcaa aatgaatatc gaaggaaacg
    43921 aagtaacttt cactcgtcca aacgacagta aagaaatgaa aacaattcat ggaacaactc
    43981 gtgcgaactt caacaacatg gttgtaggtg tcagcgaagg tttccaaaaa gcgttagaac
    44041 taatcggggt tgggtaccgt gcacaattac aaggatcaaa acttgtattg aacgttggtt
    44101 actcacatcc agtagaaatc acaccaccag ctggagtaac tgttgaagta ccagctaaca
    44161 cacaagtaat tgttaaaggt gcgaacaaag aagaagttgg cgaattagct gcaaacattc
    44221 gtggaactcg tcctccagaa ccttataaag gtaaaggtat tcgttatgtt ggcgaatatg
    44281 tacgccgtaa agaaggtaaa actggtaaat aatcgtttag aaaaattgca tagaagccag
    44341 cagcaggaaa aaggcatttg gctgaagttt accttcaaaa aaatacattt ttacttgtgc
    44401 cgcagcttct atttttcgaa acgtatttac tgcttgaaat agtagtacaa taaaaataaa
    44461 gaggtgacaa ttgtgattac aaaaccagat aagaacaaaa cacgtcaaaa gagacatcgt
    44521 cgtgtacgta acaatatctc tggtactgct gagcgcccac gcttgaacgt ttttcgttct
    44581 aacaaaaaca tctacgctca aattattgat gacgtagcgg gtgtaacgct agcaagtgcc
    44641 tctaccttgg ataaagaaat ttcaggtgga acaaaaacag aagcagcaca agctgtcggt
    44701 aaactagttg ctgaacgtgc agctgaaaaa ggcattaaag tagtagtctt tgaccgtggt
    44761 ggataccttt accatggccg tgtgcaagct ttagctgaag ctgctcgcga aaatggacta
    44821 gaattttaga agaaggagga ataccattca tggtttatat cgatccaaaa catttggaat
    44881 tagaagaccg cgttgttgca attaaccgtg taacaaaagt tgttaaaggt ggacgtcgtc
    44941 tacgttttgc tgctttagtt gttgtcggtg acaaaaacgg acacgtagga tttggtacag
    45001 gtaaagctca agaagttcct gaagcaatcc gtaaagcaat tgaagatgcg aagaaaaact
    45061 tagttgaagt acctatggtt ggttcaacaa tcccacacga agtaatcggt gcttttagtg
    45121 gtggtagaat cctaatgaaa cctgctgtag aaggttctgg ggttgctgct ggtggaccag
    45181 ttcgtgccgt attagaatta gctggggtag cagatatcac ttctaaatct ttaggctcaa
    45241 atacaccaat caacgttgtt cgcgcaacag ttgaaggttt gaaacaattg aaacgtgcag
    45301 aagaagttgc agaacttcgc ggcaaatcag ttgaagaatt aatcggataa ggaggacaac
    45361 aataatggct gaattaaaag ttactttaaa acgcagtatt atcggacgtc ctcaaaacca
    45421 acgcgataca gttaaagcgt taggtcttac aaagattaac agttcagtgg ttaagccttc
    45481 taatgaagca attaaaggca tgatcaatac tgtttctcac ctagtggacg ttgaagaaat
    45541 ttaatttaga aaaatcataa ctagaacatt aaggaggtgc caaacctatg aaacttcatg
    45601 aattaaaacc tgctgaaggt tcacgccaag tacgtaaccg tgttggtcgt ggtacttcat
    45661 ctggtaacgg aaaaactgcc ggacgcggac aaaaaggaca aaaagctcgt tcaggcggcg
    45721 gtgtgcgttt aggatttgaa gggggacaaa caccattgtt ccgtcgttta ccaaaacgag
    45781 gattcacaaa cgtgaaccgt aaagactatg cagtcattaa tcttgatgta ttgaaccgct
    45841 ttgaagacgg agctgaagta actcctgttg ctttagttga agctggaatc gtgaaaaacg
    45901 aaaaagctgg aatcaaagta ttagctaatg gggaactaaa caagaaatta actgtgaaag
    45961 cagcaaaatt ctctaaagca gctcaagaag caattgaagc ggctggtgga tcaatcgagg
    46021 tgatctaatg tttagactat taaaagatgc ttttaaagta aaagacatta gatcgaaaat
    46081 cctatttact gtatttgtgt tgtttgtatt tcgcctaggt gcacatatta ctgtaccagg
    46141 cgtaaatgct gcttcattgc aaaacctttc ttctttgcca tttttcaaca tgttgaactt
    46201 ggtaagtggt agtgcgatgc aaaacttttc cattttctca atgggggttt caccatacat
    46261 tactgcttct atcgtcattc agcttcttca aatggacatc gtgcctcgat ttgttgaatg
    46321 gtcgaaacaa ggggaagttg gtcgtaaaaa actgaatcaa gcgactcgat atctgacatt
    46381 agtattggca tttatccaat ctgtcggtat cactgctgga ttcaatcaat acgctcaatt
    46441 aggtttcgtg aataatccta acgtagcaac ttacgtaacg atcggtttga ttctaacagc
    46501 cggtacgatg tttgttactt ggctaggaga acagatcaca gaaaaaggta tcggaaatgg
    46561 cgtatcaatg atcatttttg caggtatcat ttctagattg cctgaaagta tcagagaaat
    46621 ttatgaagat tactttataa atattgatcc ttcagacata tggaaatctg ctatttttgt
    46681 ggccatttta ctagtagcgg tcttgttgat cgtgacattc gtaacatttt tccaacaagc
    46741 tgaacgcaag atcccgatcc aatatacgaa acgcgtagct ggcgcgccta caagcagcta
    46801 tctgccgttg aaagtaaatg cagctggagt tattccagtt atctttgcta gttcgtttat
    46861 tgctacgcca aatgctgttt tacaagcatt gagcggtaga ttcggttctg aaaactggta
    46921 caaagtgatg atggagatct ttaactataa tacagcacct ggtgctacga tttatacgat
    46981 tctgatcgtt gccttcactt tcttctacgc ctttgttcaa gtaaaccctg agaaattagc
    47041 ggaaaacttg caaaagcaag gaagttatat accaagcgta cgccctggta aggggactga
    47101 agagtatgtc tcaagattac tgatgcgttt aagtacagtt ggtgcagtct tccttggttt
    47161 agtggcattg cttccaatca ttgctcaaat ggtttgggat cttccacaat caatcggatt
    47221 aggcggtaca agcttactga tcgttatcgg tgtagcttta gaaactgcga aacagttaga
    47281 aggcttaatg ctgaaacgta aatatgttgg attcattgag taagaaagcg tgttgaggtt
    47341 tcccctcaac acacgttctc acacatttat taggagggaa aacaatgaac ctcattttaa
    47401 tggggctgcc aggcgcaggt aaaggaacac aagctgaaaa aatcattgat gcttatggta
    47461 ttccgcacat ctcaacagga gatatgttcc gcgcagcgat gcaaaacgaa acagctcttg
    47521 gcctggaagc aaagtcttat atggataaag gtgcattagt tcctgatgaa gtaacaaatg
    47581 gaatcgtgaa agagcgacta gctgaaccag atacagagaa aggcttctta ttagatggct
    47641 ttccacgtac gctagatcaa gcaaaagctc tggacgcaat gctaaaagat ttgaataaaa
    47701 aaattgatgc tgtcatcgat atccatgttg gtgaagaaat cttggtcgaa cgtctagccg
    47761 gccgatttat ctgcagcaat tgtggagcaa cttaccataa aattttcaac cctacaaaag
    47821 ttgaagacac atgtgatcgt tgcggtgggc atgaattcta tcaaagagaa gatgataaac
    47881 ctgagacggt taaaaatcgt ctggctatca acatcaaaaa tagtgaacca atcttggctt
    47941 attataaaga acaaggtttg ctaaacacta ttgatggtgc tcgtgaaatc gatgctgtat
    48001 ttgcagatgt acaaaaaatc attgaaaaat agcacgattg cccgtagccg acttgcatta
    48061 tgcagttact cttgtcaagt tcaataaatt atgatagaat atgtcagtcc gacttctaga
    48121 gaatcctcta gatgatccat tttgagaatt ttgaggtggg aactgcagtt tccgccattt
    48181 tatcgtgtga aaatgcgaaa caagtacaag gaggtactgt gcgtggcaaa agaagatatg
    48241 attgaagtcg aaggtacagt cgtcgaaact ttgccgaatg caatgtttaa agttgaacta
    48301 gaaaacggac accaagttct tgctactgtt tcaggtaaaa ttcgtatgca ttacatccgt
    48361 atcttacctg gtgacaaagt aacggttgaa ttgtctccat acgatcttaa ccgtggccgt
    48421 attacttatc gctttaaata attgtactcc gtaaaactac aggaggtatt atcatgaaag
    48481 taagaccatc agtaaaacca atgtgcgaac attgtaaagt aattcgtcgt aaaggacgcg
    48541 ttatggtgat ttgcccagca aatccaaaac ataaacaacg tcaaggataa ttggaggtgc
    48601 tataaatatg gctcgtattg caggagtaga tattcctcgt gataaacgtg tagtagtttc
    48661 acttacttat atctatggta ttggtaacac tactgctaaa aagatcttag ctgatgctgg
    48721 tgtatctgaa gatgttcgcg ttcgtgaatt aacgaatgaa caaacagatg ctatccgtgc
    48781 agaagtagat aaattaaaag ttgaagggga tcttcgtcgt gaagtgaact tgaacatcaa
    48841 acgtttgatg gaaatcggtt cttaccgtgg aatccgtcat cgccgtggat tgcctgttcg
    48901 tggacaaaac acgaaaaaca atgcacgtac tcgtaaaggc ccagctcgcg ctatcgctgg
    48961 caagaaaaaa taattcttaa gtgaaggagg ttaaaacttc atggcagcaa aaaaagtgaa
    49021 tcgtaaacgc cgtgtgaaga agaatataga agcaggtatt gcacatatcc attctacatt
    49081 caataacact atcatcatga tcactgatac tcacggaaat gcattagcgt ggtcatcagc
    49141 tggttcatta ggtttcaaag gaagcaaaaa atcaactcct tttgccgctc aaatggcagc
    49201 agaaactgct acaaaagcag caatggaaca cggattaaag actgttgaag taacagttaa
    49261 aggacctggt tctggacgtg aagcagcaat tcgttcatta caagctacag gtttagaagt
    49321 gactgcaatt cgtgacgtga ctccagttcc tcataatggt gcccgccctc caaaacgccg
    49381 tcgtgtttaa tgagtgccgc tcattttgag ttttatcagt aacgtcattg aacaagacac
    49441 ttttcgtttt gaaaggggta aagataagaa tgattgagtt cgaaaaacca agaatcgcaa
    49501 aaattgatga agaaaaagat tatggcaagt tcatcgttga acctcttgaa agagggtatg
    49561 ggactacttt agggaattcc ctacgtcgta ttttattatc ttctttgcca ggtgctgcca
    49621 tcactaatat tcaaatcgat ggtgtactgc atgaattctc taccatcaaa ggtgttagag
    49681 aagacgtcac tcaaatcatc ttgaacatta aaggcttagc gctgaaaatg tatggccaag
    49741 aagaaaaaac ccttgaaatc gatattaccg gacctgctac agtaactgct ggcgatatta
    49801 tcgttgatag tgatgtagaa attcttaaca aagatatgta catctgtagt gtctctgaag
    49861 gagctacgtt ccatgctcgc ttaacagtga aacctggacg tggttatgtt caagcagatg
    49921 aaaataaaaa agaagatatg ccaattggcg ttcttccagt cgattcaatc tacacacctg
    49981 ttcgtcgtgt gaactatcaa gttgagaaca cacgtgttgg acaccgtgaa gattttgata
    50041 aattgacgat ggagatttgg acagatggtt caattgaacc gttggaagca atgagtttgt
    50101 ctgcgaaaat catgactgaa catttggata tttttgtgaa cttaactgat gaagcaaaaa
    50161 atgctgaaat catggtcgaa aaagaagaaa cacaaaaaga aaaaatgttg gaaatgacga
    50221 tcgaagagtt agacttgtct gttcgctctt acaattgttt gaaacgtgca ggaatcaata
    50281 ctgtacaaga acttacaaat aaatctgagc ctgaaatgat caaagtccgt aacttaggac
    50341 gtaaatcatt agaagaagtt aaagcaaaat tgcacgactt aggtttaggt ttacgtaaag
    50401 aagattaaaa ccttttacga aggagggaaa ccaacgtgag ttatcgtaaa ctaggacgca
    50461 catctagcca acgtaaagcg atgttgcgtg atttaacaac tgatttatta atcaacgaac
    50521 gtattgtgac tactgaagct cgtgcgaaag aagttcgctc aactgcagaa aaaatgatca
    50581 ctttaggtaa acgcggagat ttgcatgcac gtcgtcaagc tgcggctttc gtacgtaatg
    50641 aagttgccag cgtgcgtgaa gaaaatgagg acatcgtcat tgaatcagct ttacaaaaac
    50701 tgttcaacga tattgcacct cgttacgcag aacgtcaagg cggatacaca cgcattttga
    50761 agacagaacc aagacgcggt gatggtgcac caatggttgt cttagaactt gtctaaaatc
    50821 acactaataa catcacttta tagatgattc ttttagagtg ttatgatgtt ggaagatatt
    50881 tttccgagtc tagctcagcg ctgctcctca agcgaatatc gcttggggag taagcactca
    50941 tcataaagtg tttttttata ccttcaatta cctgtcatga cagatatgtt tgaaggcata
    51001 aaaaaacatt ttaagcgaga aataattgaa aattttgtct ttttttgata gtgttagtag
    51061 gaactttagg atgaagaagg agtaatcatg gagccaatta ttgaattaga aaagataaat
    51121 tataaatacc agccagatga tcttcgcccc gcattgaaag atgtctcctt tacaattgat
    51181 aaaggtgagt ggatcgctat tattggacac aatggttcag gaaaatcgac acttgccaaa
    51241 acgatcaatg gattgcttct gccagaatca gggatcgtga aagtgggaaa tcaaatattg
    51301 gatgaagaaa atatttggac gatccgacag atggttggaa tggtttttca aaatccagac
    51361 aatcagtttg ttggttcgac agtagaagat gacgttgctt ttggtcttga aaatcaaggg
    51421 attcccagag aagaaatgct agtgcgtgtg aaagatgcac ttgagaaagt tagaatggct
    51481 gagtttgctt cacgcgagcc tgctcgttta tcaggagggc aaaaacaacg tgttgccatt
    51541 gccggcgtgg ttgccttacg acctgatatc attattttag atgaagcaac cagtatgcta
    51601 gatccagaag gtcgtgaaga agtgatctct acgatcaaga agatcaaaga agaaagtcaa
    51661 ttgaccgtta tatctatcac tcatgatatt gatgaagcag ctaatgccaa tcggatacta
    51721 gtgatgagac aaggcgaact tgtccgtgaa ggtacaccaa aagaaatttt ttctgctggt
    51781 cctgaactga tcgacttagg tctagatcta ccatttcctg aaaaactgaa gagcgcactg
    51841 aaagaacgtg gggttgatgt gcccagtgaa tatatgacag aagaaaggat ggtggattgg
    51901 ttatggacat ccgttttgaa caagtagact tcacttatca gccgaataca ccatttgaac
    51961 aaagagcctt atttgacatc aatatgacga tcaaagaaaa cagttatact gcattagtag
    52021 ggcataccgg aagcgggaaa tcaaccttac ttcagcattt gaatgcgctt gttaagccaa
    52081 caagcggtac agtccatatt ggagaacgag atattcagcc ggatacggat aataagaatt
    52141 taaagcccat ccgaaaaaaa gttggcatcg tctttcagtt tccagaagca cagctattcg
    52201 aagaaacggt agcaaaagac attgctttcg gtcctaaaaa ctttggtgtc agcgaagaag
    52261 aagcactagt cctagcaaaa gaaacattag aacaagttgg gctggatgaa agctatttgg
    52321 aacgttcgcc atttgaactt tcaggagggc aaatgcgtag agtagcaatc gctggtgtgc
    52381 ttgccatgag gccggaagta cttgtattgg atgaaccaac ggcaggactt gatccccagg
    52441 gcagaaaaga aatgatggag atgttttggc gtcttcataa ggaacagcag atcactattg
    52501 tattagttac gcatttaatg gatgatgtag ccaattttgc agattatgtc tatgtgttgg
    52561 aaaagggacg aatcgttaat agcggggagc cgcaagaggt ctttcaaaac attgaatggc
    52621 taaaagaaaa acaactaggc gtacctactg ccaccgagtt tgcagaagaa ttaatggcta
    52681 aagggatgaa ctttgctacc ttgcctttga cagctgaaga attagcagac gctatcgtcc
    52741 attatgctgg aggaaatgcc catgatgaat aaattgatat ttggtcgtta tatgcctgga
    52801 gattccttga tccatcgatt ggatccccga gctaaattat tagccagttt ttattttatc
    52861 ggaatcattt ttttagcaaa taattgggaa acgtaccttt tgttggcatt tttcacatta
    52921 ctttctgtct tcttgtcaaa agtagatttg aaatttttta tccgaggcat tcgcccattg
    52981 atctggctaa tcctcttcac tgtagctttg caggtcttgt ttacaagtgg cggagaaact
    53041 tattggagtt ggggtgtttt ccatatcact gaatttggtg tgatgaacgg cctctttatt
    53101 ttctgtcgat tcgtgttgat catttttatg tcgactctac tcacgcttac tacaccgcct
    53161 ttggaattat cggatgcgat tgagtacttg cttcgtccat tgaaagccgt acggttccca
    53221 gtccacgaaa tttcattgat gctgtcgatt gctcttcgtt ttgtgcctac attgatggac
    53281 gaaacagaaa agatcatgaa cgctcagcgc gctcgcggtg ttgattttgg tgaaggaaac
    53341 ctagttcaga aaatgaaagc agtcattcct ttgctgattc ctttatttgt cagcagcttc
    53401 aaccgtgcgg aagatcttgc gactgctatg gaagcaagag gctacaaagg tggggaagga
    53461 agaacgaaat atcgtgtatt gcattggcat aatagagaca cgatggttat cattgctttt
    53521 gtattattaa ctgtgctact gatcttttta agaggataaa cgttcagtgg gccgctatct
    53581 attcttttct tgctgatctg tttgcaagaa aggatgcaag cagtgtatga caagaaatat
    53641 tagggtctgt ggcagactaa ggtcacaggc ctctttcttt tatctgaaaa gaaagaacga
    53701 aaaaatttta tagaaaaatc attattccag ataacctttt aggaacaaag gagagaaaaa
    53761 catggtacgt tacaaagcaa tcattgcgta cgacggaaca aattttaatg gattccaaaa
    53821 acagccaaat gggcgtactg ttcaagaaga agtagaaaaa acattacaaa aaatggcaaa
    53881 tggaaaagaa atcacggtct ttggttcggg aaggacagat gccggagtac atgcgatcgg
    53941 gcaagtaatc cattttgatt atccagagga gcgtccgtta gaacgaatgc gtttcgcgct
    54001 agatacccag tcaccagagg atattgctgt aagacaagta gaaattgttt cagaagactt
    54061 ccacgcaaga tatttggtca aagaaaagac gtatcaattt cgagtggata tagggaaacc
    54121 acgcagtcct tttcgacgcc attatgcaag ttattttcct tacccacttg atttggaaaa
    54181 aattcagcgg gcgcttcctg atttattagg gactcacgat ttcacctctt tttgtgcatc
    54241 aggaagctcg atagaggata aaatacgtac gatctacgag gctaagatgg aggtaaatga
    54301 aacaaaagac gaattgttgt ttacatttag gggaaatggc ttcttgtata agatgatacg
    54361 gatcttagta ggaacgcttt tgaaaattgg caatggccga ttgcctgaag atagtatccc
    54421 agatattatt gcgaaaaaag atcgtaatgc agcaggacct acagcacacc ctgaaggact
    54481 ttatttgtat gaagtgaaat atgaataaga ggttatgata gaagtggtca gcttcaagaa
    54541 ataagaaggt atttacgaaa attacttctc aaaattttgc aaaatttcgg cttattttcg
    54601 aagaagctac ttctggaaca ccgtttaagg aagaggtcgt gaaatcagcg ttttgacctg
    54661 agtattagag aaacaaacgg gaaaatagct cctcatattt tgccgtttgt tgagctaata
    54721 ccgaaggtca gctgattgaa caccgtttaa gaaagaggtc gtgagcctgt catttaactc
    54781 tgagtcacaa agagcaattt caaaaaacag cttctcatct ttgatgaata tcgtgacttg
    54841 tggacgaggc gttgataagt gaatcatctt tttgttacct attttttctg taaccaaaaa
    54901 acgatggaac gttgttttga ttgcccttgg tgcggacagt ctgaacaggt tccatcgttt
    54961 ctttattttc tacgtcttct tctgtgtgtg tttcgtggtt gttcttctat tgctggatca
    55021 atcgtgccat aaaaggggcg attgcggtca aggtaccaga cataaagaat aaacaatgca
    55081 atacatagga caaaaaggat tgcacctatc gtaaatcctt caggcaagta actgaaagtg
    55141 acagtatgtt tacctgctgg aacttttatc atcaagaatg cgtctttgaa attgctgacc
    55201 ttgactggtt tcccatcaat tttagctttc catcctttgt cataaggaat agttgtcacc
    55261 aacatggaat cttcagcggt atttacagtc ccagaagctt ttctgccagt ggcttccatt
    55321 tcgacacctt tttctttgat ctgattcatt gcggcttgat aggctacaac atctagacca
    55381 acgactttag gctccatgaa acttatttct ttcgtcccat aaaaacttgc agtaaatgtc
    55441 actgttgtct cttgaggata atagccaaga tcatagtatt gaccagaaat gttgatttgc
    55501 gtttttcgcg atgtaccatt gacagtgatc gtcgcactag aactttctaa ttgcgcaaaa
    55561 ttggttggga acaaacttaa gtaagcttgt gtatttgcag gcacatggac ttcccaagtg
    55621 agctcttttg gctggttatc tgctgtttct gagtaagtga cgccagtcgt tgtctttgtg
    55681 atcgtcgtat tcaactgatt cttcaaagta ggtgtataga atgtaaaata ctgttcattt
    55741 agaccagaca atgtattgat caagtttgtc tgactggtca aattatctgt cactggctgc
    55801 tcaacatcat taacggacaa cggtgcagta aatgctagtg gcaatgcatt gacattctga
    55861 tacagtgtat agtcgccgct ttgaccagct tggcggaagc catatttact gaacgtgctt
    55921 tcgtttttag aaatgttata tttgattcct gtaaacccat ccatcagcaa agtattattg
    55981 ttataacgga tattcaaatt tgtccctctt gaacggaaac ctaatttatc caggtaagag
    56041 gaagaattac gatttcttat agaagaaaac aaactgatac cactgtaacc atagttgatc
    56101 gaatcgtttg cagatactgg atccagattt tccaagcggt aaaacgagtc ggaatctttt
    56161 tttgtttcat ttacaagagc tttcaagtca ggataaggat cagtatataa actgcgggaa
    56221 gcataattcc aatcatcaag aatgccatgg atcatactgt tcgtattgac cagtgcttca
    56281 gcagacatga tcaataataa caatatactc agatgattga atgaaagttt tttatgctga
    56341 taaaatccca cagcaaaagc ataaatccct aagaaaacca ctgtcaatac aaaggaagtc
    56401 atcgtcacat aggtgtagct tccttcaggt ttgatgcccc atgccaccgc aaagagagca
    56461 gctacgacca aaatgattcc taacaacagc ccacgatcgt cttgttctat cttttcccat
    56521 ccttttcctg caagcaggag cactaagaaa gagaacagat aggcataacg gaataaaaac
    56581 atattcggtg catgcatacc atgccagaac aaattcaatg gaacaagata aaaacttgcg
    56641 ataagtatgg cgaaaagact gccgtacaaa aatttatttt tcaacggtac tttccgactg
    56701 gcaaaataaa acagacagag gactaatggt agcagacctg cgtaaacgaa cggaatcgat
    56761 ccgtattttg tcgtgtcata aacaccaatc atatttttca tgattagatc cagataagaa
    56821 gtcgcttctg ttttcaacgt agtgatctga ctcagtgcct cgccatttga ccgaagatcc
    56881 agcactgctg gcaatacaat gatcattgaa gcgccgcctg ccagtaaaga agtgatacca
    56941 taaggaacaa tcgttttttt atattctgac cagtttgtca atagacgggc gataaaatat
    57001 aagaaagaaa atacgccaat cataaaaccc atataaaaac tggataaaaa caagagtaaa
    57061 taactgataa ataaaagttt cggccgttta agatccataa cacgatgaat ccccaaaatc
    57121 actaatggaa gataaaaaaa tgcatccagc cacatgatga tctccgaatg tgcagtgata
    57181 aatgagatca atgcgtaact gacgctaagt gcaacatgcc cccagcgagg aatcttgaac
    57241 gtacgtgcaa gaaaccaaaa agccaatccg gcacagccga ccttaagcaa agtcagaaca
    57301 tacagtgcat ctggcatcat ctggtttgga aaaaagaaca ccagtggcgt gaagatcccg
    57361 cctagatagt aagaaattaa agataaataa ttcaatccta gagaagcatt ccatgtataa
    57421 aaaatacttt gctttccaag aagaacattt cggaagctgg catgaaaatt agaaaactgt
    57481 gaaaaggcat cacttgccaa aatacttctg ctgcttccag gatagattcc gatacttaga
    57541 taaacaagtg ctaaaatcaa aaacggaatc aggaaactag ctgctgcgta gcgtccgttt
    57601 tctctaaaga atgactttaa attttttttc atatttattt tccaccaaat ttctatgaag
    57661 ttgtattcag tttatcatat ccttgttttt ctctgcttaa aaagagtcta gttttcacaa
    57721 aaaaataaaa tttagaataa ataacttcat aatttgttcg tataacaaaa ataaacgcaa
    57781 aaaaagggta ggaatcaaaa gtcccaccct taaaaatttt tattaaataa aaaaccgttc
    57841 actatctgaa cggtccaagt atcattcaat taaacgcgtt caacttttcc tgatttcaaa
    57901 gcacgagttg acacccaaac ttttttaggt tttccgtcga ccataacgcg aactttttgt
    57961 aagttaggtt taacagtacg ttttgttgag ttcatagcgt gtgaacggtt gtttccactt
    58021 tttgtcttgc gacctgtaaa gtaacaaact tttgccatgg taatttcctc ctttgccttg
    58081 atttggagca atagaataat tcagctcata ccagactaac atatcatata gaatgggaat
    58141 ttgcaagtac attttcatag atacaattga caattgagaa gaataattgt aaattaaaca
    58201 aggttatacc tgaaattttg caaggttttt gagtactttg ggtaactgcc ttttatttca
    58261 agttgcgcta tgataaaata tgatagaaga aaaatgggag ttttttaccc gttaaggagg
    58321 ctcattcatg gctgtaaaaa ttcaaacgtc agctggaacc attgaaatta ccaatgatgt
    58381 aattgcaaca gttgttggtg gcgccgcgac agatgtgttc ggtgttgtag gaatggccag
    58441 caaaaatcaa attaaagata atattactga gattttgcgt aaagaaaatt actcaagagg
    58501 agtagttgtc cgccaagaag ataatggtat tgctgtagat gtttatacga ttatcagcta
    58561 tggtacgaaa atctctgaag tttcccgcaa cgtccaagaa aaagtgaaat acaatctaga
    58621 ggcattatta ggcgtaactg ccaattcagt taatgtcttt gtacaaggtg ttcgtgtact
    58681 gccagactag gcaggagact ggctggtaac tcagcagtcg atctgaagga ggattatcta
    58741 ggtgagtgta acagaaataa gtgcaagcca gttccaggaa atggtccagg caggtgcgaa
    58801 tcgtctgcaa gtaaatgcag aatacgtcaa ttcattaaat gtattccctg tgccagatgg
    58861 cgatacggga acaaatatga atctatctat gactagtgga gctaaggcag tagtagattc
    58921 aacctcagaa aaagtaggag aattagcagc aatgctttca aaaggattgc tgatgggtgc
    58981 gcgcggaaat tccggcgtta ttttgtctca attgtttcgt ggattttcta agcaaattcc
    59041 agatgttgtc tcattaaatg cttctgatct tgctgcagcc ttcactcatg gagttgaaac
    59101 tgcgtataaa gcagtaatga aaccagtaga aggaacgatt ttgacagtag ctagagaagc
    59161 agcgaaagct ggagaaaaga aagcaaaaag tactgatgat gtcgttgaag taatgacagc
    59221 tgttgtaaaa ggagcaaagc gtgctttagc taaaactcca gacttgcttc cagtcctaaa
    59281 agaagtcggt gtcgtagaca gtggagggca aggactgctg tttgtttatg agggcttttt
    59341 aagtgcattg aatggagaat tccaagcgga tgaaacatat gaaccttctc cagcagaaat
    59401 ggatgatatg gtcaatgcag aacatcatcg cagtgtccaa ggtcaaatgg cgacagaaga
    59461 tatccatttt ggttactgta cagaaatcat ggtaaaaatc ggagaaggac ctacagtcga
    59521 tagtacattc gattacgaag aattccgtaa ctatttagat ggtataggag attctttact
    59581 tgtggtcaat gatgacgaga tcatcaaagt ccacgtgcat acagaaaacc caggagaagt
    59641 cttgaattat ggacaaaaat tcggttcatt agtcaaaatc aaagtcgaca acatgcgctt
    59701 gcagcatgag acgattttgg aacacgatga agaagtgaac gagtttgcga aaaaagaaat
    59761 accacgtatc ccgtatggta tcgtagcgat cgctgctggt aaaggcgtcc aagagctgtt
    59821 tgaaagttta ggcgctaatt atgttatcag tggtggacaa acaatgaatc caagtacgga
    59881 agacattgtt aaagcgattc aagaagtaaa cgcagataaa gtgattgtct tgccaaacaa
    59941 taagaacatc ttcatggcag ctgatcaagc agcagaagta gcagatatcc cagtagcagt
    60001 tgttccttca agaacgattt cccaaggatt gacagctatg ctttcattca atgatcaagc
    60061 ttctttagaa gaaaacaaag aaacgatgac agaaatgttg tctagcgttg tgagcggtca
    60121 agtcactcac gcgatcagag atacctcaat tgacggtgta gagatcaaag aaggagactt
    60181 tttgggcatg atcgatggaa aaatcctgat ctctaaccca gatatcacag caacagcgtt
    60241 aagtacacta gaacaaatga tcaatgagga cacagagatc gtaacgatct tggcaggtga
    60301 tgaaggaaca gttgaacaag caaatgaatt tgctgagaaa ctaacagaaa aacatcctga
    60361 tttggaaatc gagatccacc aaggagatca gcctgtctat ccttatctat tttctgctga
    60421 ataatttctg tgtcagaata tctttctaac acagtattca ttcataaaag gccttcggtt
    60481 agtgatcagg tatgatcgct gaccaagtcc tttttttgtt aaaggaggga ggaaaaattt
    60541 atgcgttctt tagatgatcc aatcactatg ctggcaggag tcggagaaaa aagagcagct
    60601 agtcttgcca gcttgggcat ccaaacgatt gaagacttac tgacttatta tccttttcgc
    60661 tacgaagata ttcaggaaag aaatctaaac gagatccagg atcaagaaaa ggttactttg
    60721 aaaggaatcg tagtctcgcc tcctgtgatg aatcgatttg gttataaaaa aagccgattg
    60781 caatttcgta tgatgcaaga acatgatgtg ttcaacgttt ctttttttaa tcaaccttat
    60841 ctaaaagaaa aagtcatttt atcggaggaa atcgccgtat acggtaaatg ggatgcaaag
    60901 cgtaaagcct tgaacgggat gaagatttta ggttctcaaa caatggatga cttttcgccg
    60961 atatatcacg tcaacaaatc aattcgtcaa acgacattag ttgatctaat caaaaaaggg
    61021 tttcaagaat acggcacatt gattgaagaa aatctgccaa acaaccttgt ggagaaatat
    61081 cgactgcttg accgagcttc tgctgtaaga agtatgcatt ttccaaaaga ccacgaagaa
    61141 aaccatcaag ccaaacgacg tgtcgttttt gaagagttct ttttatttca aatgcgtctt
    61201 caaggattga aaaaagcaga aaaagcagaa acgaatggtt tggagatcct ttatgatgtc
    61261 caaaaattga aacaatttac tcaaaagcta ccttttgaac tgacaaatgc acaaaaacgt
    61321 gtaacgaatg aaatctgtcg ggatcttcgc agtccgcagc atatgcagcg acttttgcag
    61381 ggagatgtag gtagcggaaa aacagtggtt gccgcgattg cgttatacgc taccgttaca
    61441 gcaggtttcc aaggtgcgtt gatggtgcct acagaaatct tggcacaaca acatatggaa
    61501 agcttgcaac agctgtttga tcccaatgaa gtaaccatcg cattgattac ggggtcaacg
    61561 aaagcaaaag aacgacgtga gctccttgaa aaactagagc aaggggaaat taacgtcgtg
    61621 atcggcacac atgcattgat ccaagatgga gtcgaatttc agaatttagg tctagttatt
    61681 acggatgagc agcaccgttt tggcgtgaac cagcggaaag tacttcgtga aaaaggctgg
    61741 cgaccagatg tcttatttat gacagcgact ccgattccta gaacattagc tattacagca
    61801 tatggtgaga tggatgtatc tatcattgat gaacttccag ctggacgtat cccgatcgaa
    61861 acacgctggg taagaacacc tcaattggac agtgtactgg attggacgaa aaaagaactg
    61921 gcaagaggac atcaaatgta tgtcatctgt ccgctgattg aagaatcaga agcattagat
    61981 gtaaaaaatg cagtggaaat ctatgaaaaa cttcgtgaac tatttgcacc aaaatatgaa
    62041 gttggcttgt tacatggtaa aatgaagaat caggaaaaag aagtaatcat ggaagccttc
    62101 aaagacaacc agatgcagat attagtttcg actactgtaa ttgaagtagg ggtaaacgtg
    62161 ccgaacgcga ccgtcatgct gatcatggat gcggatcgat ttggattggc acagctgcac
    62221 caacttcgtg gacgtgttgg acgcggaagt gatgcttcct actgtatctt agttgctaat
    62281 cctaaaaatg aactaggtgt ggaacggatg aaaatcatga cagagaccac caatggtttt
    62341 gtactaagcg aaaaagatct ggaattacgt ggtcctggtg aagtatttgg cttccgtcaa
    62401 tctggtctgc ctcaatttgc gattgctgac ttggtgacgg atggaaatgt tttggaggta
    62461 gccagagaag aggctgcagc cctttggcaa ataaaggatt ggcagatgct tcctgagtat
    62521 cagcctttag cgcaaagcct ccaaatagaa aatcaggata atcgttattt cgattagcag
    62581 actgtgctat actttatact gatgacggaa aaaggagtgg aaacaatgaa aattgccgta
    62641 gatgcaatgg gcggagatca cgcacctcaa gcaattgtag aaggcgtggc tttagcgcag
    62701 caagattttc cagaagtaga attcttactt tacggaaaag aagcagaaat caagaaatac
    62761 ttaacaaatg aaaaaaacat cacgattatc catacagacg aaaaaatcaa tagtgatgat
    62821 gagccagtaa aagcaattcg acgaaaaaag actgcatcaa tggtccttgc tgcacaagca
    62881 gtgaaaaacg gtgaagcaga tgctgtattt tcagcaggga atacaggcgc gctactagca
    62941 gcagggttgt tcatcgtagg acgtatcaaa aatatcgaac gccctggctt gatgtctacc
    63001 ttgccagtag tagggaaaga aggtgccggt ttcgatatgc tggatcttgg cgctaacgca
    63061 gaaaacaaac cagaacattt gttgcaatac ggtattttag gttcttttta tgcacgtaaa
    63121 gtccgaggta tcggacgtcc tcgtgtcgca ctcttaaaca acggaacaga agcaacaaaa
    63181 ggtagtgaag tgacgaaaaa agcctatgaa ctccttcaga atgaaacaag cttgaatttt
    63241 attggaaacg tagaagctcg tgagctactc aatggtgtgg ctgatgtagt ggtcacagat
    63301 ggatttacag gcaacgctgt tttgaaatct attgaaggaa cagcaatgaa tatgatgaat
    63361 ctattaaaat ctgctattct aaatgaaggg atcaaaggaa aaatgggcgc tatgctttta
    63421 aaagacggct tacgttcttt gaaagcagaa atggattatt ccaaacatgg cggtgccgtt
    63481 ttgtttggct tgaaagcacc agtcattaaa actcatggag caacaggccc cgatgctgtt
    63541 cgctatacga tccgccaaat ccacacaatg cttgagacag atgtagttgg acaattagtc
    63601 gaacaatttg aaaaaagcga agaatagtat ttggatatct gaagcgatcc gttccattga
    63661 aaaaaaggtg gacattcctg aaaaatgcca gtaaaattag ccatggtgta ggtaattgac
    63721 aagtgtatga ttcttggagg tgaaaaagtt gactcgcgaa gaagtattta acaaagtagc
    63781 gaaaattatt tcaaatcatt ttgagataga taccgataaa gtgacggatg aattgaacat
    63841 caaagatgat ttaaaagctg attcaattag tattatggaa ttcgttttag agcttgaaga
    63901 tgaattcggt acggaaattt cagatgaaga cgctgaacaa attgaaacag tcggtggcgc
    63961 agtagattat atcagcagcc atcttaaata atttcaaata aaagcaaaga aggataaaaa
    64021 gctgcatgca gctttttatc cttctttttt taatcttttt gacacgtaaa agtcattttt
    64081 ctgataattc tggcgataaa cagaaattat gttgcaaag
//
LOCUS       DAMYXM010000010        59668 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.010, whole genome shotgun sequence.
ACCESSION   DAMYXM010000010 DAMYXM010000000
VERSION     DAMYXM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.010"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(507..1817)
                     /locus_tag="PNS21_000744"
     CDS             complement(507..1817)
                     /locus_tag="PNS21_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDI5158476.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1829..3247)
                     /locus_tag="PNS21_000745"
     CDS             complement(1829..3247)
                     /locus_tag="PNS21_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5158477.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3487..5055)
                     /locus_tag="PNS21_000746"
     CDS             complement(3487..5055)
                     /locus_tag="PNS21_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDI5158478.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5059..5469)
                     /locus_tag="PNS21_000747"
     CDS             complement(5059..5469)
                     /locus_tag="PNS21_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDI5158479.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5466..6389)
                     /locus_tag="PNS21_000748"
     CDS             complement(5466..6389)
                     /locus_tag="PNS21_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HDI5158480.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6386..7264)
                     /locus_tag="PNS21_000749"
     CDS             complement(6386..7264)
                     /locus_tag="PNS21_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HDI5158481.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7251..8588)
                     /locus_tag="PNS21_000750"
     CDS             complement(7251..8588)
                     /locus_tag="PNS21_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDI5158482.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8591..9694)
                     /locus_tag="PNS21_000751"
     CDS             complement(8591..9694)
                     /locus_tag="PNS21_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDI5158483.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9768..11276)
                     /locus_tag="PNS21_000752"
     CDS             complement(9768..11276)
                     /locus_tag="PNS21_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDI5158484.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11242..11934)
                     /locus_tag="PNS21_000753"
     CDS             complement(11242..11934)
                     /locus_tag="PNS21_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDI5158485.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11956..12930)
                     /locus_tag="PNS21_000754"
     CDS             complement(11956..12930)
                     /locus_tag="PNS21_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDI5158486.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13230..14102
                     /locus_tag="PNS21_000755"
     CDS             13230..14102
                     /locus_tag="PNS21_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDI5158487.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14560..16758)
                     /locus_tag="PNS21_000756"
     CDS             complement(14560..16758)
                     /locus_tag="PNS21_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5158488.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16990..17982
                     /locus_tag="PNS21_000757"
     CDS             16990..17982
                     /locus_tag="PNS21_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI5158489.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            18042..19463
                     /locus_tag="PNS21_000758"
     CDS             18042..19463
                     /locus_tag="PNS21_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDI5158490.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19456..20955
                     /locus_tag="PNS21_000759"
     CDS             19456..20955
                     /locus_tag="PNS21_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDI5158491.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20994..22388
                     /gene="uxaC"
                     /locus_tag="PNS21_000760"
     CDS             20994..22388
                     /gene="uxaC"
                     /locus_tag="PNS21_000760"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDI5158492.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22547..23230
                     /locus_tag="PNS21_000761"
     CDS             22547..23230
                     /locus_tag="PNS21_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HDI5158493.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23232..24068
                     /locus_tag="PNS21_000762"
     CDS             23232..24068
                     /locus_tag="PNS21_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDI5158494.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24079..25824
                     /locus_tag="PNS21_000763"
     CDS             24079..25824
                     /locus_tag="PNS21_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5158495.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIELAP
                     IARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFTGIL
                     ALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVETYH
                     GKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTLLNL
                     LIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKNVSK
                     ERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKIILLD
                     EATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIGTYE
                     KLLAQKGYFYRLKNE"
     gene            complement(26100..26441)
                     /locus_tag="PNS21_000764"
     CDS             complement(26100..26441)
                     /locus_tag="PNS21_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDI5158496.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26509..28680)
                     /locus_tag="PNS21_000765"
     CDS             complement(26509..28680)
                     /locus_tag="PNS21_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDI5158497.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28849..29748
                     /locus_tag="PNS21_000766"
     CDS             28849..29748
                     /locus_tag="PNS21_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDI5158498.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29819..30676)
                     /locus_tag="PNS21_000767"
     CDS             complement(29819..30676)
                     /locus_tag="PNS21_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDI5158499.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30661..33363)
                     /locus_tag="PNS21_000768"
     CDS             complement(30661..33363)
                     /locus_tag="PNS21_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDI5158500.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33376..34665)
                     /locus_tag="PNS21_000769"
     CDS             complement(33376..34665)
                     /locus_tag="PNS21_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HDI5158501.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34815..35861
                     /locus_tag="PNS21_000770"
     CDS             34815..35861
                     /locus_tag="PNS21_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI5158502.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35863..38016
                     /locus_tag="PNS21_000771"
     CDS             35863..38016
                     /locus_tag="PNS21_000771"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HDI5158503.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38489..39940)
                     /locus_tag="PNS21_000772"
     CDS             complement(38489..39940)
                     /locus_tag="PNS21_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI5158504.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39937..41661)
                     /locus_tag="PNS21_000773"
     CDS             complement(39937..41661)
                     /locus_tag="PNS21_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDI5158505.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41654..42268)
                     /locus_tag="PNS21_000774"
     CDS             complement(41654..42268)
                     /locus_tag="PNS21_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDI5158506.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42613..44070)
                     /locus_tag="PNS21_000775"
     CDS             complement(42613..44070)
                     /locus_tag="PNS21_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI5158507.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(44111..45031)
                     /locus_tag="PNS21_000776"
     CDS             complement(44111..45031)
                     /locus_tag="PNS21_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDI5158508.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(45043..45990)
                     /locus_tag="PNS21_000777"
     CDS             complement(45043..45990)
                     /locus_tag="PNS21_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDI5158509.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(46469..47188)
                     /locus_tag="PNS21_000778"
     CDS             complement(46469..47188)
                     /locus_tag="PNS21_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDI5158510.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(47237..48883)
                     /locus_tag="PNS21_000779"
     CDS             complement(47237..48883)
                     /locus_tag="PNS21_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDI5158511.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            49062..49652
                     /locus_tag="PNS21_000780"
     CDS             49062..49652
                     /locus_tag="PNS21_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDI5158512.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            49744..51249
                     /locus_tag="PNS21_000781"
     CDS             49744..51249
                     /locus_tag="PNS21_000781"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDI5158513.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(51333..52685)
                     /gene="celB"
                     /locus_tag="PNS21_000782"
     CDS             complement(51333..52685)
                     /gene="celB"
                     /locus_tag="PNS21_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDI5158514.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(52685..53203)
                     /locus_tag="PNS21_000783"
     CDS             complement(52685..53203)
                     /locus_tag="PNS21_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158515.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(53219..53545)
                     /locus_tag="PNS21_000784"
     CDS             complement(53219..53545)
                     /locus_tag="PNS21_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HDI5158516.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(53558..55537)
                     /locus_tag="PNS21_000785"
     CDS             complement(53558..55537)
                     /locus_tag="PNS21_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDI5158517.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(55558..55872)
                     /locus_tag="PNS21_000786"
     CDS             complement(55558..55872)
                     /locus_tag="PNS21_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HDI5158518.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            56040..56333
                     /locus_tag="PNS21_000787"
     CDS             56040..56333
                     /locus_tag="PNS21_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDI5158519.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(56411..57523)
                     /locus_tag="PNS21_000788"
     CDS             complement(56411..57523)
                     /locus_tag="PNS21_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDI5158520.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            57910..58089
                     /locus_tag="PNS21_000789"
     CDS             57910..58089
                     /locus_tag="PNS21_000789"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158521.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            58283..58453
                     /locus_tag="PNS21_000790"
     CDS             58283..58453
                     /locus_tag="PNS21_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI5158522.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(58877..59617)
                     /locus_tag="PNS21_000791"
     CDS             complement(58877..59617)
                     /locus_tag="PNS21_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDI5158523.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        17831 a        12190 c        10433 g        19214 t
ORIGIN      
        1 cgattgttaa gctcaatttg tttgctagca tattgcttgc ttgttaaaaa tgtttattgt
       61 cttgaattga atcaagacgc cctacacatt tggtttgtct tactttgttc agttttcaaa
      121 ggtctacttc atcgagagtg tttcccgatt catttttggc tgccgtagca acttttatat
      181 catatcagag agacaaacga ttgtcaactc ttttttaaaa gtttttttcg atttcgtttt
      241 tcgaaatctt tcagcaactc atttatcata acttatcgtt tgttatttgt caagaacttt
      301 tttgaagttt ttttcgatag aatcaagctt cgcaagtggt tctttttaac gacttagtta
      361 tcttatcacg tctaaaaaca tgtgtcaact cttttttgga attttcataa aatgtttcaa
      421 tgaagcgcga caacgttgtt taatatatca ttaaaaaaaa taaaggtcaa gctaaaatat
      481 tatttaagct tgaccttatt ataatttcaa cgattgtcaa aagtattgtt tgcagctgtg
      541 ttgccagttt ctatagataa atcacagctt ttgaaacata agttttgtac tgcttcgatt
      601 cgcccgaatt cttttgcagt tatttggcaa tgagaaaagc agacatcttc tataggcttt
      661 tcaggaaatg ctactaaatc aaaagctcgg gaaggaagtg agtaacctgg agaaaggcat
      721 gcctgtacgt ttttgatgtg tatgtctttg actttcgtca tttgctttga ttcaggtatt
      781 tcttcagcca ctgcttgcca actttctggc atttgagcaa ggtcattgct attttttcta
      841 ttgtattcat tatgccagtc cattatccaa gaaaaaggaa attgtacatc aatcatattc
      901 agattttcta ccctgatatt ttcgatcact ccccctcttt cttttgaaga tttcattcga
      961 ataccacagt ctgactgaaa aaaatcgata tcgtgaatat atacgtcact aactccagca
     1021 ctaacttcgc taccaatagt tacaccgtat cctgaacgga ttttacatct agcaacctcg
     1081 attcttgatg attttcgatt gactcttgca ccattgccat ctctgccaga cttaatagca
     1141 atacaatcat caccacaggc caaatcacat tcatagacac gcacatttgt tgaagaatca
     1201 atatcaatcc catctgtact tggtccatca ttgttgcgga tgattacttt ttctactaat
     1261 acatcgttag aataagtgat ttgcaagttc caaaaacctg atttctgaaa aatcaaatca
     1321 cgaacaacaa catgttctgc atgatacagt aaacaagcgc gtggacgttt aatagcataa
     1381 tccgcaatcc aacgaagtcc cttttgatcg tattcagctc ttgtcccgct tttttgatct
     1441 tttccccaat acagctccca ccaatggtct ccttgaccgt cgattattcc ttttccttct
     1501 ataagaatat ctttcgccga gagaacattc aaaatcgctg ctggccattt catttctacg
     1561 ccggctactc gtgtatcgat ttctggaaat gcttcgatat ctgttctccc cttgaggact
     1621 gcaccttctt caaagataag atgactctta tcttttaaaa aaagggctcc gactaaataa
     1681 atgccttttg gaataaatac aggtaagtgt tctttcgcac cttgatcgat tgctttttgc
     1741 agtattattg tatcatcttg ttgagtgtcg tttgttttaa agttcaaagc attgataatc
     1801 cctttttttg tccgcaatac gtttctccct atctttcatt gatttttttc aattcttcac
     1861 caataaatac agtttttgaa cgattcagtg ggtaacctac tagcaaaatg attgcggata
     1921 caaataagca gaatgctggt aacaagtttg ccatcgtata aatcctttct tgtactaaaa
     1981 ctgattggat ggctccacct gttgtagacg attggtaacc aatgagtgcc agcataaatc
     2041 cgcctaccgc tcctgcacat gcttgtccta cttttctcgc aaaagaattt actccatata
     2101 ctgttccatc ttctcttaag cctgtcttat attgatgata atcaatcacg tcagtaatga
     2161 atgcccagac catcaaattg aataaaccag atcctaaagt agcgataaat aaaagaatca
     2221 gatataccca tgcatttgtt atatgaagga aatacatcag caagtacata catgaagaag
     2281 caagtaacgc aaatacgctt gcttcctttt taccaaataa ttttgtcagc tttgtagcaa
     2341 atggtgctaa aactactact gttccgtaag taaataaaag agcaacagac attgcttgtt
     2401 tattatggaa gtaatcattg aataaataag tcatattcgt cccagctaaa atctgattga
     2461 tcacaatgaa aatgtcaaca atgactaaaa taatcagtgc tcgattagct aacaaacctt
     2521 ttaccaactg agaagcagat actttttcgt ttttgacaac tcttactctt tcagtagtca
     2581 atttacaagt taacatatat gcgacaaatg ctattgcagc acatccgatt gaaatccaaa
     2641 agaaatgatt ccctgaaacg acttgttgtc cactcttgtt atgggtatac attaaaatcg
     2701 gaatgaagaa tcctgtagtt gatccgccaa tcgcagaacc aatgcttcgg aacgtggaca
     2761 acgaggtgcg gtcattagga tttgagctaa ttgctgctgc cattgctcca tacgggatat
     2821 tgattgttga gagaaaaatc ccataaacaa tgtatgaaac aaaaatataa gcaatcttca
     2881 ccgtcattgg aagatgttgg acgaatggca aaaacaaaat gacagtcact aagcaaaatg
     2941 gatacttcat ttttctgatc caaggaataa atcggccagt ttctgttaac tctgagacat
     3001 cacataatct tccaaccatc acatcagcaa acgcatccaa aattctcgaa atcaagaata
     3061 acgtacctac gattgcccct gaaacgccaa gaacattcgt ataataaatc atcaagaaac
     3121 tattgactaa acctaaaata aaacagttac ctagatcacc gaacatgtag cctatcttat
     3181 cggaaatccc aaattccttt ttttcttttt ttattgtatt tgctgtaaca gcattttgtg
     3241 aaagcatttg aattcctcct acctcttcta gtaaacgttt tcaaaattat aactcttttt
     3301 ttcttgtttt tatattcaca atgttggctt tttattgcat tatcttgaca gataaaaaac
     3361 aaaaaataat ctaaaaacat tgatgcataa gattgataac tttttttaca taacaatata
     3421 tacaaacaaa aaaattcact aactagtgaa aataagttag tgaacctcat ttattaaaga
     3481 attaaattac agatgatctt ttccatcata cttgaaaaac tcaaaatcag cataacttcc
     3541 tcttttttta tttagatcat gcactgctat cccgataaaa tttccagtaa accctcctga
     3601 gagtatacac atgttttttt gcggacctac tttcttccat tcttcagttg atacgtcttt
     3661 caaatgaaac tgtgctgtct ctttctttac agtgactttc aaaaaagtct tcttttctgt
     3721 gagtggaatc gtccaatctt ctatttgtgc tactccgttt tctgattcta tcatacgtag
     3781 gcattttccc ttgtcctcat tccaagtaac ataacaataa atataattgg attcatttaa
     3841 gtacagtacc agtccagcca tctgattaaa attattaggc atgtattcaa atgctgtctc
     3901 cgcatcaaaa gtaaaatccg tttgccgaat agctaacaaa tgatgttcga aggtagattg
     3961 aagagattca ccggaaatca gacgtaaaaa acctggtcta cattttgtat cacaccaatc
     4021 agcatttggc aaaatgcgtc tggcattcca gttcttcttt agctctcctt caaatgtatc
     4081 cataaactga tgatcttttt cttgttgtac ctcttcattt gtctcaatca ttatttcttc
     4141 catcggagtt gtctggctat ctttaagtct taaccaatcg tcggacgtcc aatacacttc
     4201 ttgaatagcg gtttctcttc ccaaaatagc cgcaccgttt aatggtctgg tacaaagata
     4261 tgtcatgtac catcttcctg acggggtttg tactaagctc ccatgtcctg tacattgcaa
     4321 tgtagaagca ggtttgtcac tagctgtcag cataggaaaa ccaggatcaa gctcgtaagg
     4381 accagtaata ttctttgctc ggcaaactgt aactgaatgt cctcttcctg taccgccttc
     4441 tgccgtcatc aaataataat acccgttacg aaaatacaaa tgtggtgcct ccgttttcgc
     4501 taattctgta cctgcaaata ttttatagat gggaccatca agcttcttct tctctgcatc
     4561 atattcttgt aatacgatac cgctggattt attcggtgtg tccagacgat aatcccatag
     4621 ttcattgaca agccattttt ttcccgtttc aagatcatgg aacaaagaag gatcaaagcc
     4681 gttactattt aaataaattg gttcggacca aggacctgtg atctcagatg cagtgatcaa
     4741 ataattgtgg acatctttaa acggacggcc tgcacttttg acatctgtgt aaacaagata
     4801 aaacaatcca tcagcataac tgatctgtgg cgcccatata cttccatttg taggatttcc
     4861 ttgcaaagat acttgagtgg tcaaaatatc cgttgtatgt gaccagttga ctaaattttt
     4921 cgactcatat actcggattc ctggaagcca ttcaaaagag gagacaataa tgtaataccg
     4981 ctctttcact cttaatatat taggatctgg attgaatcct cttaaaatag gatttttcac
     5041 tttgatagtc tgcatgattc aggctccttt tctcattctt actcgatata aactgaatgc
     5101 cccagctgcg aaataaaaaa atgcaaaagg aaacgcaata aactgagtgc caatcaacaa
     5161 tatgaatcct gttataaaat acaaaccagt ggcagttgtt aagtattttc ctttagcagt
     5221 caaaaacaat gctactgcta aaaaagcaag tgaaataaac aacacaacgg caaacagacc
     5281 agtcatgtcc tgtaataatc caaatgtatt ttctaaagac aaagttgaat gatctacagc
     5341 aagcgactga taactttttt taaactctga cgcagacata ttacttaaag taactgctaa
     5401 cccgccaaag aaaatcaaag atagactgcc accgaccatt cctagcaata gttcccattt
     5461 tcttttcatt tctgaacccc ctgcttttct aaatgtatcc attcaggtct tttgatcgat
     5521 gtttcataat tgttattacg ttcagagaat ttgacagtct gttcattttt cttgtcatcc
     5581 caattatccc aaccagttgg gtgaacatgc ccacctatct tacacgcttc aaataagaca
     5641 tttgcatacg gacgccacgg tcttcctaaa tagacgtttc tagtatcagg ttctgcagtc
     5701 aaggtacagc tttcaaaacg ataccctgtt ttttgatttt tcggcgtaga tgctgctgtg
     5761 atgtatccag gatttttact ggctaagcta tggatatgac actgatgaaa aacggcatct
     5821 gccccaccaa atatgaaatc caccgttcct tcaatccagc aatattcata ggtctgacga
     5881 caatactcag gattggcaga agccggtgta caaaaaggag taccatcttt ttgcaaatca
     5941 ggcaacggac ccgtgcataa ggtatcctga taaccagata gtcgacagtt ttttaaagat
     6001 acgcgatctc ctgaattgaa taaagcaaca gcctgtccta ccttttctcc tggacctgct
     6061 gtattttcga tgtgaagatt ttctaatacg acattcgatc cttctaagaa aaaagtagca
     6121 cttcggaaag tccctaactg gctaccgtca agatactcct gttttgcaaa acgattaccg
     6181 gagataacta ctgttcctgc tcccacaagt ttaaaattgg ataatctgct ttctatatat
     6241 tcatcatatc ttccttcttt tactatcaag acctttggat ggctatccgg caggcctgcc
     6301 aaataatcga ttccttcttg gatagtttca aaatccgtcg gggtatctat agcattcttt
     6361 ccaattatca gtttgtcttt catcctcata aatgctccct tatgaattgt ttccaaatat
     6421 ttctcatttc ttgtgtttcc atatgaccgc ctgttaaaga aaaagaagaa aagttttctg
     6481 gactacccat tgattgatac tttttgtcca gcccttttct cagcttcagt acgccttcgt
     6541 ccaagcacat cgtatcgttt tttccaacta agctaagacg agggcgcgga gcgatttttt
     6601 cttggattgc taacgttgta taagctgtca atagatttgg cacatagtaa taaaacccat
     6661 gatgatctag gcggcgatgt agcaaaagta tttcgatgtc cacctgtgcc gctagatcaa
     6721 tacaaacttt gacacgagaa tccaatgctg tcacccacca gcttaataag cctcccatag
     6781 aaagaccaat cgttgcgagc cgttgagtat ctgtgtcttc ccttgtctcc aaataatcga
     6841 tcaagctgat cacatcataa atacgcattc cccataacgt ttttcctgtt aataaaaatt
     6901 gtttgaacaa ctcgctttca gaaatacctc cgcgttcttc aaatccccaa gcatcaatcg
     6961 accaaacagc atatcccatt cctgtcagta cttctgcaaa agatggagaa gctaagtaag
     7021 gagcaccatg gatcaattca cttttcccga ctgaaaaatc tccgccgtga gaatgcaggt
     7081 aaaggaccgg cggaaacggt ccttcttttt ctggataagc atagagggct ggtactgttt
     7141 ccaattcatt taagtacaat tgatagtatt caagcataaa accgttttgt ttttctcggt
     7201 ataatcgttt tccactaggg gtatgtctgc tagatggagc acctaaaaaa tcattcaact
     7261 gcataagcaa agtttttacg aacaaccagt tcatccatat tttctgattg atttgattgg
     7321 aatttctcaa tactgatttc tttagtattt tctaatagaa atgctggtcc ctcgtgattt
     7381 tcaatcgaca tctggttaaa ggaaacctta cccgcaaagc caatataaaa tccttgtctg
     7441 ctcatttcct ctattcctgt catcatagca ggaacaccgg gaactgcatc ttgggccatc
     7501 gagacatcga tattgtcaaa tgtgatttct tgtatgaatt gttccgctaa tccataaata
     7561 aaacctgcag aagcatggac attccttgcc gtgatattcg aaaaatggat ccttctgaat
     7621 gctggtgtcc gttcatctat cggataagcc tttttttccc atacatacgg ttctttccct
     7681 cgcggaccac agaaataata aagattcagt ataaatggac acatcacatt atccatgact
     7741 atattggata ctcgaatatc ttcaacgatc ccgcctcgac ctcgtctaga tttcaatcgt
     7801 attcctcgat cagtttcttg gaagatacaa tttgaaatgg taatattacg aatactacca
     7861 ctcatttcac ttcctaatac gacaccgcca tgtccatgga ccatcgtgca atttgtgatc
     7921 gtgatatttt cgcaagcgat cctttcgtac gtatcttcag tccctgcctt gattgcaata
     7981 cagtcatctc ccacgtcaat atggcaatta ctaatacgca catttttgca tgattctgga
     8041 tcaattccgt ctgtatttgg tgaatccgct gggttcaaga tcgtcaagtt atcaaacgtt
     8101 gcattactgc aaagaatcgg gttgatcgtc caacttggtg actggatcaa tttaatatct
     8161 ttcacagtaa ttcgatgaca gttatggaaa ctcatcaatt taggacgagg ataagcgaga
     8221 ttatctggtt catttctaaa agtatgccac catttttttc catttccatc taatgtaccg
     8281 aaaccagtga cagagatgtt ctctacattt tgagcataga tacaggaagc ataaactttt
     8341 cgatggactc cttcccaacg tgaatgaaca acaggataat cttttggatc gtcagagaat
     8401 ttcagaaccg ctcctgcgct taaatgaagt tcgacattgc tttttaaaaa taaagctcct
     8461 gttagaaatt cacctgctgg aacaactacg gtcccgcctc cgtcacttgc tgcagcatca
     8521 atggcttgtt gtattgcttc agtattcaat tcatcaattg atgcgccaaa tgtcaaaata
     8581 tcatacatat ttataatgcc tccacttcaa tcaatgcttg caagaatgca ccaacgcctt
     8641 tttgatcatt acaaataatt ggttcactga tgtagtaagc atatgaacca tctcgctgat
     8701 ctgctccgcc taaccctgct acttgacagt ttttattcaa gttcagccag ccttcttttg
     8761 tttccagaac aaattcatca agtaatcctt gatgtgattt cttcacttgt ggaagccatt
     8821 cttctttgtt cagcagtccc aaacgtattc ctttagccat tgcgtaaaca aacatactac
     8881 tgccggaagc ttccagataa tttcctttcc gttttgtttc tgttgttact tgataccatg
     8941 tcccgctgtt ttcttcttga tatggaagta atgcagccag tgtcttttct agaatttctt
     9001 tcagcaaaga acaatctgtt tcttcatgaa gtaattcata ggtatctgct gcagccatca
     9061 aataccagcc aatcgatcgg ctccaaaaat gatgggatag acctgttttt ggatcagccc
     9121 atggttggac cttcttttca tcccatgcat gaatcagcaa atttgttttt ggatcgaata
     9181 gatgtttata actgatttca aactgtctca aaacatcttg gtaatctttt tcttcactaa
     9241 aattctggat aaattcagca taaaaaggag aacccatata taagccatcc aaccaaattt
     9301 gataaggata aatttcttta tgccaaaaag ctccttcgga tgtgcgtgga tggtttgcca
     9361 actgctggga aaggagttct gctgcttttt tatatttttc ttcgcctgtt tctttgtaca
     9421 ataaaaagaa aagctttcct gtatttacat ggtcgatatt gtattcttca acgctgtagc
     9481 cacggatcgt tccatcttct tgcacgaaat agtcgatatt gtcttttatg aaatcaaaat
     9541 aaattttttc ttgggttcgt tcccaaagta attggaaccc tttaagtacg acgccatagt
     9601 cataagacca ttttccttta taccactttt cttcatataa gcgaggtgtt ctttccatga
     9661 tactttttgc taatttcttt gaccagtttt ccattatttg ctctccttta aaaattaaaa
     9721 taggatttgg gacaaatacc aagaagtatt tgtcccaatc gtctatatta ctgtgccgct
     9781 gctttgtatg cttcctcgta ctcttgtgtg attcgatcgc ctccttgtgc tttccagtct
     9841 tctacagctt ttttgaatcc atcttcgtca agctgtccca tgatgtattg caccgtagca
     9901 tcaatgacga ttttttcaat ttctgatcct acttcattgt tcgtcggtga atccaatggc
     9961 acagaaggat ccattacagc aaattcttca ttttctttga tcaattgatt agagaattct
    10021 ttttcagggt ctgtatcttt gaaagagaac gtcacttcac taggacgaga gcttgataac
    10081 ggttgcacct cttgttgcca aagatccata tcgataaatt cgatagaacc atcatcattc
    10141 aattcataat gcgttccttc gataccgcca gtcatcaatt tatacacatc ttcttctaaa
    10201 agatcatcaa caaattgaag taatttcttc aattgtgctt cgtcttttac ttgagatttg
    10261 ggaaatgcta gcaagccgcc aataccattt ccttctgacc aaacatgata accgccgcct
    10321 tcaccatctg agatcttgtt tacaggcact aattccatat catcttgaat accttgaccc
    10381 atcgtcttca gattcttgat atccaccatt ccagtgtaga ttccagttct tccttgtgca
    10441 aattgttctt gctgatcagt cttttgggta actgcaaagt cttgtgccaa atagccattc
    10501 tcaaatagat ctcttgaaaa attcaaagct ttcatatact cttctgtatc aaattctgga
    10561 gtgaatttgc cattatcgtc tacagcccag ccatttggtg ctccaaaata agaagtgaaa
    10621 agtttaaagc tggtataacg aatatcacta ggcgcacgat cgccaaaacc aactgtgtcg
    10681 tccacaccat ttccatcagg atcgttttct gtaaattgtt tagctacttc ataaagctca
    10741 tccatggtag ttggaacatc tagtcctaga ttatccaacc agtcttttcg aatgactaag
    10801 ccagcacgag cgtagtcttt ttgtatcggt acgccgtaca aggcaccgtc gatactcgca
    10861 gcttcgattc tatcttcaga aatttgactt aaatttttat agtcttttaa atattctgat
    10921 acatcccaga atacgcctga ttttaacgca ttgcggacag aagagttttt caacatcgca
    10981 atttgaagtg taacgatatc tcctagttca tcagaagcca atgcagttgt taatctttct
    11041 tcttttgaag catcaggaat ccagttgaat gtcagttccg tatcggtttt ttcttcgatc
    11101 aagttcacaa tcgtgtctgt aggcggtgaa gcatgatgca aaatatttaa ccaagtgatt
    11161 ttagcttttc catcttctgt ctctgaagaa ctgttacttg aactgctgcc gcatgcggca
    11221 agtgctagaa ttcctgcaaa actaagacct aattttacga gcgttgcttt tttcataaat
    11281 tttctccttc actgttttaa taatattttt gttttcttct gttttttctg tatatgagtt
    11341 cttccattcc aaatggcaaa atatagaatt tgctgtccac gtgactgcgg aaaagaagag
    11401 actgccaccg ataaaggtta aaacacctgg cattgtttgg atgatcatca gataaatccc
    11461 taccatactt aatatcaaca ctaatgagta atgaagatgt gagaatccga ataaaaatgc
    11521 cattttcatt ttgaaaaaat atcctttcca atcataatga gccatgattg ggaaaatata
    11581 gagtgctgag agcacatata tgaacgcagc aatatacaaa gcgatcctaa gataccaatt
    11641 gtgtatgaaa gaaagatcca caagtaaaac aatcccgatg gcgccatata tccagctcat
    11701 cagcaaggtt tcacgatagt tttctttaaa atatgtgaag tacgttttcg tcaaaggaaa
    11761 cgtttctttt gagcgcagcc attttctaat cactgcaacc atcgcatacg atgcaggacc
    11821 gaaagtaaaa actcccaaac ctgcgatgac taaaccgccc cacagaaaat tcaaataaac
    11881 caactgtaaa atacgattta gccttccgtt tattttatct aatgactgca gcatatcttt
    11941 ccctctcttt ctatcttagt aaacgccgtc ttctcctagc tttttggcaa tcttattagc
    12001 taaaattacc ataaacaatc ctacgacccc tttaaacaat cccattgctg tactgaaact
    12061 taactgtcca ttcttcaatc cagctgtata aatataggtg tcgaaaattt ctgcaacgct
    12121 tcgattcaat gaattcaata acaagtacat atgctcgaac cctaagtcta atgtctgacc
    12181 gatttttaga atcagcaagg taatgattac tggacgaatt gccggcaatg taacatgcca
    12241 tgttttgcgt aagcgtcctg ctccatccat ttctgcagct tcatacaact gtgtatcgac
    12301 tgcagttatc gctgaaaggt agatgatcgt agaccagccc aactctttcc agatcacttg
    12361 tccgatatat actgtgcgtg tccattcagg ggatgttaga aaactgattt tttcaaatcc
    12421 cattgttgca aggaattcat tcaatacccc tccgtcaaca ttcaaaaata cataagtaat
    12481 cgaaacaatg attacccaag acatgaagtg agggatgtaa atgatcgttt gaatcccgtt
    12541 tttcaatcgt ttgttcgtta cttcatttaa tagtaaagac aaaatgattg gcatcggaaa
    12601 aaacacgaag atatttaatc cgaacaagat caacgtattt cttaataatg taaagaatgt
    12661 cggttctgta aacaaacgaa tgaaatgttt gaaaccgacc caaggacttc ccattattcc
    12721 taaaaatggt tgataatctt gaaaagcaat aacgagtccc cccataggaa gatacctaaa
    12781 aatcaagaaa taaaggactc ccggcagaat catcacatat aaaaatttat tcgttttgat
    12841 tcctgcaatt attttttttc gtcttctgag cttttcttct tttttcgata gcgtcagttg
    12901 aaatggttct tgtttcttta cactttccat aagcccctcc ttagttttgt gaatgtttgc
    12961 tgcttatgac ttcatgatag ataactatct gaattacgta tataatcatt tttaccgtaa
    13021 gtaaaaacgc ttacatagca atgatttttg tgctatacaa aacgataaag tgtatttttt
    13081 cacaaataaa ctacgcatag caaaccttct atttttttct atctgacact tagcatagga
    13141 aatgattata tacgtaatct ttcaccgatg ataaattgaa catatagaaa cagaaacatt
    13201 taatttattt tgaggaggag cctatctata tgaaaaagaa agtctctata ggagaaagaa
    13261 gttttacact tttcaacaat acgtttctaa ttttgctagc tttgatctgt atcgttcctt
    13321 ttttaaatat catcgctaca tcttttgctt ctacacaaga agtcgtcgcg aaaaagttta
    13381 tcctttttcc tactaccttt tctttagacg cgtatcgcta cattctttct acgccgacca
    13441 tttttagagc acttgctgtt tcgattggtg ttacaggtgt cggaacgatc gtcagtatgt
    13501 gtgcaacttc actgatggcc tacggtcttt ccagaaagta tttgtttggt cgaggtttcg
    13561 tcaactttct tgttgtattt tctatgttgt tcagcggggg gatgattcca acgttcttag
    13621 ttgttcgttc tctagggctg gtcaattctt actgggccat gatcctgcct gtggctgtta
    13681 atgcaatgaa tatgatcatt atgagaaact ttttccaagc cttgccggat agtttggaag
    13741 aatcagcaaa aatggatggt tgcacagatt ttggtgtatt tttcaaaatt atgctgccat
    13801 tagcgttacc gtctattgct actatctcgc ttttttatgc tgttacttac tggaacactt
    13861 atatgactgc tattttgtat atcaacgatt catctaaatg gccgatacag attcttttac
    13921 gccaaatcgt cattgtatca agtgggatgc aagcagaaag tagtgccgtt gatgtgattc
    13981 caccagcaca aacagttaaa atggctgtta tcgttattgc aacagtgcct atgctgattg
    14041 tttatccttt tgttcagaaa tattttgtca aaggagcact cgtaggttct gtcaaagggt
    14101 gattatgtac tgaacgcaaa atggtagttg tgtcagaagt ggtcagcttc aagaaataag
    14161 aaggcattta cgaaaattgc ttcttaaatt tttgtgaaat ttcggcttat ttttcgaaga
    14221 atctactcct ggaacaccgt gtatcagagg ttgtgaaaga gacgttcagc cttgagcgat
    14281 gataaaaact ctcacaaaca ttgcgacgag taacccacag gagcaataag aagcgatttt
    14341 gaaaaatagc ttcccatatt tcacaaaatt atgccttatt gtcgatagcc tgtctcttga
    14401 acgccattta ttcgtaaaaa agaactgtga caagactttt gtcacagttc tttttgcgtg
    14461 cttagccttg aaatttaact atccataaat acaaatacta gggttattct aacttctttt
    14521 attgcttgaa tcagtccaca agaaaaacag cctcttgatt cacaaatatt tttttcgata
    14581 ttctcccggc gtaatcgatt ccttcttttt aaagaaacga ataaaatttt gcggatttcg
    14641 atattgcaat ctttctgcaa tctcttttac ggagaggtta gtctctttca accatttttt
    14701 tgctacctct aaacgatgat tttgaacaaa atcaccaaat ttttctcctg tttccttttt
    14761 aaatacatta cttaaatagt tagggttata atgcaaccta tcagcgatca catctaaaga
    14821 cagatcacga tcatattctg tgtagacgat atgcataata ttctccgaca aatttttaaa
    14881 ttcatgatca gtgattgctt gagagcattc tgtgattggc agaactaatt gttctaaaac
    14941 aagtttttct agttctttgg gatgataatt gttcagtgct tttacataaa tttgtttcat
    15001 gttatcaaag ttttttgaat ctactcccaa taactgtcct aattgaacga gttcattgat
    15061 caagcggacc gaaactactt cccgactcaa aggattttta ttttgtgcaa acaatgcatc
    15121 aatcaattca tgtactaaat tcttcacttc ctgatttcca gaacgaatgg cttcaaacaa
    15181 tcgattttgt tcttctatcg gatatctaat aagtgttttt tcatgagcag caggaacgat
    15241 ttcctgataa aaaataattg aacttggacc agtatttaca cgataataca gggcttcttt
    15301 tgcccgatca accgattgct tgctttcatt cagcgtttcg aaccggttac tgatgcctat
    15361 actgatagtt aaattcaagt attcttttac acgtttttga atgagtcggc aatactccat
    15421 gacttttttg cttgcttctg catcatcttt atccattcga taaatagtgg cttgcatttc
    15481 ttcattcaaa acgatcggca gcattcgatt ttcctttggc acaatttccg tgatcatgtt
    15541 gttgatcgct aaaagtagca aatcacgctc cccaccgtga ctatcttcta acagatcgat
    15601 ttgaaccaaa gctgtataat agcagtcatt ttgagaacta taaccaaatt gctgcaaccg
    15661 ctgatctagt tctcgccttt ctactcgatt tctaaataaa ctaagtacaa atagcgtttc
    15721 cagctgtggc ttttgggtaa ctaagtgtgc acttaacgct tctttttctc ctatgatccg
    15781 actgactgat tgagcgacca aatctagttc tttcccactg aatccttttg atttaagatt
    15841 cagacgttcc tgtatttgag aaatcggacg ggcaaatctg tccgaaaata agtaggaaag
    15901 aagaccaatg agtcctacca gtgttaaaga cactgcaact agcccgattc tcagattgcg
    15961 gatcattgcg ccaattgcac tagagtcaac ttctaatctg taaatccaat tattataatc
    16021 tgatttcagt actatatacg ttttatcttt ttctcttatc actttcagct gattgtaatc
    16081 tagatccacg tccgttaata cagttggttg ctctttattt tttactgtct gagtagtaaa
    16141 aaggttgcca acttcgtttt caatagaaag tatgtcttct ccactttcac caacaacttg
    16201 ttctattgta tgtttctcta tcgtagcaat tcccaatgca tatttttctt tttcaaacat
    16261 tggcaaagaa atcaccattt cgattccttt gtttttcttt tgccaaaata aattattttt
    16321 gttatttatg tacttctgtt gatacttttc aatttcctct tcggtcagtt gactcaacga
    16381 tccattgatt actccccatt taccagccaa actgactaaa tcataattat tatcatccat
    16441 aacaatcgtt tctataaaat ttagttcttt cctgatttca gaataagtgg agtaatctgt
    16501 atatgtcagc ggatgcttga atatgtccga gaaatttttg gtcgaaccat acgtagaaaa
    16561 cgaatattca atcgttctaa ttttccattc aacgttagtt ttaacttgcc tgagcaaatt
    16621 ctttttgtct gttaaataac tcgattcgat cgattcatag gtattctggt aaatgaataa
    16681 actaaatcct atgacaagta cggctcctaa taaaaacatc ggtaaaaata ttttataaaa
    16741 tactgtactt ttcttcattt tcttcctctc ctccttttaa aataaaaggc tttcattttt
    16801 ctgtaaaatt gatactcatc taattaaatg agcattcccc gctttcaaga atagcataat
    16861 taaaaataaa aatccaagta atttttaacg tgtgcaatct tttcatatca aagatctatt
    16921 gacttatcgt agttatcgta atatgatgaa tgcgttaaca aaaagtgaaa aagtaaggaa
    16981 aatgatggaa tggtaacgat caaagatgtc gctaaagcag ctggcgtttc tcatacgaca
    17041 gtttctcgtg ctttgaataa cagcagttta ataaaaaatg aaacaaaaca aaaaatacga
    17101 caactagcta aggaaatgaa ttattcgcca aactatagtg caaaaggttt agtgaatcaa
    17161 aaaaaatatc taatcggact atttttctca agtttgcata aaggaacttc atcaaatttc
    17221 ctagcagatt ccattgcagg catccattca gtattagaca caaattacag tttatctgtt
    17281 gaaagtatcg atgttgttga tttaacagaa atcaacctgc aacgctacga tggactgatc
    17341 gttatgagtc aaagtgatga tgatcagcct ttcatcgact atctcaaaga aaaagaattt
    17401 cctttcgttg ttgtcaatcg tcacatcaat gatccagcgg ttatcaacgt cactgccgat
    17461 gatgcggccg gtgttatgca ggcagtagat tacggtattg gatgcggaca tacgaagatc
    17521 gcttatattg gcggaaagaa aaattttcgt tcgacaaaag aacgggaaaa aggggtattg
    17581 aaaagtctaa aaaaagcgag catccctgtc aatccagcct attttttaac aggagattac
    17641 agcttagaaa gcggcctcca aaacatggaa tacctattat cactttctga cctgcctact
    17701 ctggtttttt gcggcaacga cgacatagcg attggtgcat tgagagctgt acatttagct
    17761 ggcctccgtg ttccccagaa catttctctg atcgggttcg atgattctcc agtcgtctcc
    17821 tatctcaatc cgccgcttac caccgtacgt aagcctttga aagaaatgtg tcaagaagga
    17881 acaaagcttt tactagattt gatcgaaggc aggacgatcg aacaatctaa aattgagctc
    17941 tccaccttgt tgatccttcg tgagtcgatc aattacttgt aaactacaag taattttata
    18001 taaaaatgtt aacgtgtgca taacacagaa aggaagttta tatgaaaaaa ctgacaaaag
    18061 aattagtgaa aaaaaacgaa gcacctgtaa aagttcttca gtttggtgaa ggcaatttta
    18121 tgcgtggatt tattgattgg cagattcagc agctgaataa ccaacaattg tttaaaggta
    18181 atgttgcaat cattcaacct ttagaacaag gtcttggaaa tatgatgaac cagcaagata
    18241 atttatatac ggtcatttta caaggactca gcaatggcga aatcgttgat acttccgaga
    18301 ttatcaccgt agtagaacaa atgatcaatc cttatgatga atgggatgct tatctatctc
    18361 tagcagaaaa tgacgacttg gcctatatcg tctccaatac aacagaggca ggaatccaat
    18421 tcgtggagac tgataattta acagatactc ccccacagag cctcccgggc aaactgacag
    18481 cctttttata ccgacgtttc aaattaaaca aagctggctt taccatcatt ccttgcgaac
    18541 tgattgacag aaacggcgaa cgtctaaaag aaatcatttt acgctacgca gaaaaatggg
    18601 atttagaacc tgcttttgtt gagtggttag aaaaagaaaa tgttttctgt tgtagtttag
    18661 ttgatcgcat cgtcccaggt tatcctcgcg aagatgcagg tgttttcaat caacggcata
    18721 actatgaaga tcaattgatg gtcaaagcag aaccattcat gctttgggtg atcgaagggc
    18781 ctaaagaatt ggagaatcaa ctaccattca aacaggctgg cttgaatgtc atcatcacag
    18841 atgatatgac tccttatcgt gaacgtaagg tgcatttgct taatggaccg catacagcga
    18901 tggtccctct tgctttgttg gcggaattgg aaactgtaga agaagtaatg aaagatcctt
    18961 tattctccgc atatgttgat cagttattca atctagaatt gattcctatg ctttctttac
    19021 ctaaagatga attagccatt tatgctgacc aaatcaaaga acgtttcttg aatccttttg
    19081 cccatcacaa attagaagcg atttcattaa atagtgtttc aaagttcagc acccgtctgc
    19141 ttccagtgtt caagaagtat atcgaagaac aaaatcaggt accaccgtta atcactgttt
    19201 cactagctgc gctacttctt atgtatcgag gcgatcaagt aaagccacat gatgatgaaa
    19261 agacgataag cgaatttact gatgcttggt ctgataatgg aacagctatt cctcgattat
    19321 tgcaaaacgc tgcgctttgg ggagaagatt tgtctcaaat accgaatgtg acagatacag
    19381 ttcaagaaat agctgcaaag atcgatcaaa aaggtgtccg gtcagtatta caagaaatga
    19441 aaggaacgat ggtccatgct tgaaaccctg caaccaacaa tgaaattaaa tcgacgagac
    19501 agtgtggtcg tcgcattaac ccctattcca aaacaaacac aactgacgat tgatcagcaa
    19561 acgatcacca ctcttgaaga tatcccgcaa ggtcataaga ttgcattggg tgatttgaaa
    19621 gctggagaca acgtgatcaa atatggctat ccaatcggtc atgtgacgac agacgtaact
    19681 gccggtcaat ggctgcacac tcataatgta aaaaccaatc tttctggtga attggattat
    19741 cgatatgaaa aagatgtcca tccaagtcat tacctttttg agaaccgaac atttcaaggt
    19801 tacttgcgaa aaaatggaaa agtagggatt cgtaatgatt tgtttttagt acctaccgtt
    19861 gggtgtgtga atggcattgc tgaactgatc gtcaaacagt tcaaggaaaa acatccagat
    19921 ctgggacagt ttgaccatat tacgatttta aagcatcctt atggctgttc gcagcttgga
    19981 aaagatcatc aaaatactcg agaaatttta gcggacgcag taaatcatcc caatgccgga
    20041 ggagttttag tatttggatt aggttgtgag aacaatacag ttcctgaatt caaaaaaatt
    20101 ttaggtgaat atgatgaaga acgagtgaaa tttctagtgg ctcaagatgt ttacgatgag
    20161 atcgaacaag gtgtggctct attagaagag cttttaacag cggcggaaaa tgaccagcgc
    20221 atatcagtcc cgctctctaa gttgaatgtc ggtttgaaat gcggcggttc tgatggtcta
    20281 tcgggaataa ctgctaatcc tttacttggt gcattttctg actacttgat tgctcaagga
    20341 ggcagcacga tcttaacaga agtgccagaa atgtttggcg cagaacaagt attgatggca
    20401 cgagcggaaa atgaagaagt ttttgactca atcgttgact tgatcaatga cttcaaacag
    20461 tatttcttgt cttacgggga accagtttac gaaaacccat ctcctggtaa taaagcaggt
    20521 gggatcacta cattagaaga taagtcttta ggctgcacgc aaaaatcagg aagctctcct
    20581 gtcgttgatg tgctacaata tggcgaaaaa atcagaaaac cagggcttag tttattgcaa
    20641 gctcccggga atgatcttgt agctgcctct gctcttgctt catccgattg ccagttggtc
    20701 ttgtttacca caggaagagg gactccgttt ggctcatatg tcccaacggt caaggtatct
    20761 acgaatacaa ctctttttga acgcaaaggt cattggatgg attttaatgc aggcgtctta
    20821 ttagaacaac caatggaagt aatcttagaa tcgtttgttc aaaaaatcat cgctgtagct
    20881 agtggagaag aaacgaaaaa tgaacaaaat gacgttcgag aaattgccat ttttaaaaac
    20941 ggagtaacac tttgattttc ataaaacagt ataaaaagaa agaaggaaga tttatgtttt
    21001 tatctgatga ttttttatta aaagacgatt gggcgaaaaa gctttaccat tcctacgcaa
    21061 aaaacatgcc cattattgat tatcactgtc acttagatcc aaaagaaatt tacgaaaaca
    21121 acaatttcag caatctgaca gaagcttggt taggaggcga tcattacaaa tggcgcttga
    21181 tgcgagcttg cggcgtgcct gaagaaaaaa tcacaggaaa tgcttctgat tttgaaaaat
    21241 ttttagcttg gtgtcaaacc gtaccaaaaa ttatcggaaa tccgctttac agttggtcac
    21301 atcttgaatt gaagcgtttc ttcggtattg acttgttgat aaatgaagaa aatgcagaga
    21361 aaatttggac acaagcaaat gaaattttgg cagctcctga ttttcgccgt cgggaaatcg
    21421 caaaaaaatc gaatgtagaa gtgatttgta caacagatga tcccgcagac gacttgcact
    21481 atcatcagtt gcttgctcat gaagaaccag atttgaaggt tctaccttca ttccgtccag
    21541 ataaagcact caatattgaa aaagaaggat ttaatgcatg ggtcacacta ctggaagaag
    21601 cagctgacca aacgatcacc acctatcaag aactgataga agtcttaggg cagcggatag
    21661 aatacttcca tcaacatgga tgtcgtgttt ccgaccacgc tttagacatc ttacgctata
    21721 aagaagcgga tgaaacagta ttagaagaga tttttcaaaa agcacgagct aacaaactat
    21781 tatcttcaga tgaaatcgat gcttatcgta cagaaacact gatacatttg acccacttct
    21841 atcattctca taattggacg atgcagctgc acatccatgc ttatcgtaac tgtaatacac
    21901 aaatgttgga aaaattaggg cccgataccg gctatgatgg aatgaatgat ctttctttga
    21961 ctattcctct gcaaaaatta ttgaaccgtg cagaagaaac ggatcagttg ccaaagacga
    22021 tcctttattc attgaatccg aatgactatc ctgcgatttt agctttgatg ggctgtttcc
    22081 aaaaagaaca gaatggaaaa ctgcagttag gttctggatg gtggtacaac gatactcgag
    22141 ctggtatgcg cgatcaattg acacaatttg ctgatggtag tgctttgggg aattttgtcg
    22201 gcatgctgac agattcaaga agtttcctgt cctacactag acatgaatat ttccgtcgtg
    22261 ttctttgtga atttttaggc gaaatggtca tgcgaggaga agcacctgaa gatgaacaac
    22321 ttttaggaag tctagttcaa gcgattagtt atacaaatgc taaaaattac tttggctttt
    22381 ttgcctaatt gattaatagt aacagtaacg aatcaaaaac aggatagtga aataacggaa
    22441 tccgtttatc cacgttttag agaagaagac ggaccaaatt tgctccgtct tcttctcttg
    22501 ctgttctgct tcattgccta taaaccttta tgaaagaaga gataacatgc ctactatcca
    22561 tattgccggt gattctactg cggcagaaaa agatgaaaac aagcgtcccg agacaggttg
    22621 gggagaaaaa atctctgctt atttttcacc cgagatccag atcaataacc aagcaaagaa
    22681 tggacgcagc accaaatcat ttatccagga aggaaggtta gcccgtctag cttcacagtt
    22741 tcagccgaat gatttcttat ttgtacaatt tggacataac gaccagaaaa tccaagaaga
    22801 aagaggtacg cttcccttcg gtagctattt aaaaaatctg cagatatttt ttgaatgcgc
    22861 aaaaaaagcg aacgtccaaa tggtcttact gacatctgtg acgcgacgag attacttaga
    22921 aaacggaaca ttgaatccag atatacttgg agactatcca aaagcgatgc gtgcatttgc
    22981 tgagaaacac catattcctc ttttagatgt gttctcaaga tcacaagaat tgttccagac
    23041 cttctcaaaa gaagagacaa aaaaattcta cctgcatctt atacctgata cttgtaaaaa
    23101 ttacccagaa ggactaaaag acaataccca ctttagtcca gaaggcgctg ataaagttgc
    23161 ccgaataatt gttgaattga taaaagaatc ccgtctgcct cttgctaatt atttacaaaa
    23221 aggagtttga gatgtttcca ttaaatttag gcatccgtgc ccacgattta gatgttcgaa
    23281 atcgtgaaga aatcgttcaa aaagtatcac agcttcactt gtcccatatc caatttgcac
    23341 cacaaaaagc atttcctgaa caacttaaat taactgacat gacacttggg agtgctgcct
    23401 attttggtga atacttccaa aaaaacaata tcgaactttc cattttagga tgctatatca
    23461 acctatccag caaaaatcaa gaagttcgtg cacaagcatt ggcaactttc aaacagcaaa
    23521 ttgctctgtg ctctgcctat caagccaaga tgattgctac ggaaaccggt agtgtctctg
    23581 tcggttacac aaaagaaaat tttactgaag aagcttatca gatcgccaga aattctatca
    23641 ttgaattagt cgctcatgca gaaaattttg gcgtaactgt tgctgttgaa gcagggatca
    23701 atcatccaat ctataactat cagttagcta aacgtttaat cgatgaagta gcttcaccaa
    23761 atttaaaaat cattttagat tgtgcgaatt tgatgcataa agaaaatcat gctgatcagg
    23821 aaaaagtgat ccgaggtgcc ttggacaatt tacatggtta tattacagcg cttcatctca
    23881 aagactatat catggaaaat ggaaagatcc gcatggtacc tgttggcaag ggctgtttag
    23941 atttccgccc ggtactgcat tatatcaaat atgagcgtcc tctaatgtac gccactcttg
    24001 aagctactcc tgagatttat gttcctgaag caatagaaca cttgcagttt atttaccaag
    24061 attgttaagg agaagattat ggaggaacga atacagaaaa gttatcaaaa atatcatgca
    24121 aatgcttttc agacattgct gcatctttat cagggaaatt attggcgttt tgccatgtca
    24181 ggactctttt ttattatcaa acatcttcca tcttggttaa tgccgatcgc tattgccaat
    24241 gtaatcaatg cagcttcatc gaaagattct tctcagttgc aggccattta tttcaacgct
    24301 tttttgatgg cttgtctgat tggtcaaaat atattgacga attattttca tgtaaaatac
    24361 catagcactt cgatccgtca agtagaagct ggactaagag cgacccttat caagaaaatc
    24421 caagaactat ctattcctta tcaaaaagaa ttgcaatctg gacgaatcca gtcaaagatc
    24481 atccgtgatg tagaagctat ccaaacgttg tcctctcaag tatttgtcag cggattaaat
    24541 atcgctgtca atttattcgt agctttaggt atcacgcttt ataaaagtcc tgttgttttc
    24601 atgtttttct tattgactgt tcctctcgcc gcattcgtta ctctcttttt ccgtaaaagg
    24661 atcagacagt ccaactcaga cttcaggcaa gaaatggaag agacttctgc aaaagtgatc
    24721 gaattagcac cgattgctcg agctcatggg ttagaaaaac acgaaacaac tcgcttaaat
    24781 aagcatttta ctcaaatcta tgaaaaagga ttccacttag atatcctgca atctgttttc
    24841 ggatcagtca gctgggctag ttttcagata tttcagctga tttgtttgat tttcactggg
    24901 atattagctt tacaagggcg catccaaccc ggcgatgtcg tgatgtatca gacgtatttc
    24961 acgacgatcg tcaattccgt ttctggaatg atcacgctga ttcctacgat ttcaaaaggc
    25021 atggaatcta tcacttctat tggcgaactg ttgattgctg gcgatgtaga aacttatcac
    25081 ggtaagaaaa aaatccaaca tttggatgga aattttcgtt ttgaggatgt ttcttttctt
    25141 tatccggact cactcaaacc gattatcgat catttttctc tagaggtgaa agctggagaa
    25201 acaattgctt ttgttgggcc atctggctct ggaaaatcta cactccttaa cttattgatt
    25261 ggatttatcc agccgacttc aggaaaaatt tttctagatg atcagccttt ggatgaactg
    25321 gatatgcgaa gtgtgcgaaa ttatttagcc gttgttcctc aaaccacttt gttgttctcc
    25381 gcttcgatca aagagaacat cacttatggc ttgaaaaacg tatcaaaaga acgtttagat
    25441 gaagtcatcg aagctgcgca actttcttca ctgattgacc agcttcctga tggtctggat
    25501 accttagtag gcgaacatgg caacaagctg tctggtggcc aaaagcaacg tatctccatt
    25561 gcacgagcgt tgatacgtca gccaaaaatc attttgctag atgaagcaac ctcagcttta
    25621 gataaccagt cagagaaaaa aatccagcaa gccctgaatt acctgacaga aacacccact
    25681 acttttattg tggctcatcg gctttcaacg atcaaagagg cagataaaat cgttgtgatc
    25741 gatgaaggaa aaatcgttga aataggtact tacgaaaaac tgttagctca aaaaggctat
    25801 ttctaccgct tgaaaaatga atgacaaaaa ataggagcta tgacaagact tttgtcgtag
    25861 ctcctatttc tgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    25921 catcaaacat gagaagctgt tttttgaaat tgttccttgt aactcagagt taaacggtag
    25981 gctcacgacc tcttggtaaa tggtgttcaa gagacagcct atcggcaata agaagcaatt
    26041 ttgtcatcgc tcaaggccga acgtctcttt cacaccctct gttgtttcat aaaatagttt
    26101 tattcgctgt aaaggtcgcg aactggtgtc atgatgatac ggttcaagtc attgatgatt
    26161 gtactgaagc cttgctcttt ttgcatcaag tcattgatta cttcttctga ttggattttc
    26221 acagccattt cttgtgcttt cattgcatct tcgtcgctga actcttgtcc actcatttgt
    26281 ttttcttgta attcgatttg catcgcttgg aagtctttga acagtttgtg tgcttcttcg
    26341 tttgcttgta ctttctcgta cgcttctttc aacgctttga attctggtaa ttcacggatc
    26401 tcacgttcga tttgattcgc gctatcgtag atgtttgaca tgttatgccc tcttttcttt
    26461 tagtagttac tctctattgt aacatggggt tattcgtttt caaaggggct actctccttg
    26521 atatttccgc cagaggtctt tagccgcttc accaattccc tgaacgcctt ctttcaccat
    26581 atcgccgaag tttttcagcc cttcttttgc ttgttcacca tattgattca agttatcttg
    26641 ccaatttcca gactctccat tagaatttcc tgtgtttcca acttgatctg cagctaccac
    26701 ttgtcctcct gtggcatagg catctgctac actaaatcct gtttttgaag tatacggaag
    26761 gatgccttct gccgctgatt tgaagacttt cccgacaaca tttccacttg tcccttccaa
    26821 atagtgcagc ttgctggatt cttcgtaacc catccatgtc gaaatcacta cgtctggcgt
    26881 gtaaccgatc atccattggt catttgcttt tgtggcatcg aaattagttt ctgtggttcc
    26941 tgttttccct gctatcgtat aacctgcagg attcgcagca gccgctgttc catttgaaaa
    27001 tgtaccaaga agcatgctgg tcatttcatc ggctgtttcc tttgatatga cctgttttgg
    27061 ttttttgttt gtgttatcga cgatcaccgc tccagtcgaa tcaacgattt tcgtgataag
    27121 atgcggcgta tacatctgac catcattggc aaatgcactg taagcacccg ccatgatcaa
    27181 tggagatact cctttttcca gtccaccaag ggctaagcca taatacttat cagattttgc
    27241 taaaggcagg ccgaattctt ctgctttatc atatcctttt tgtaaaccga tctcatgcaa
    27301 aagccataca gtaggcaaat tcaaactttg tgcaacagct tgatacatcg gtacttctcc
    27361 ttggtatgtc ccgtcaaaat tatgcgcatc gtaataactt tgcggttcgt ctttcaagat
    27421 actggaaggc ttgtatccag cttccagtgc tggtgtatat acgcttaatg gtttgatcgt
    27481 tgatcctggt gaacgacgca tctgtgtagc aaagctgaat cctctaaaga catgatctcc
    27541 tcttcgtcct actagtgctt gtacaccacc tgtattcgga tctaaagcta ctgaagcaga
    27601 ttggaccatg gcaccatctg aagcatttgc tgggaataat gcactgtttt gataagtagc
    27661 ttctaattgc ttttgataat cctgatctaa agctgtatag attttatagc ctttattcat
    27721 gacatcttct tcatcaagac catactcgtt aactgcttca tcgatcactg catcaaaata
    27781 ataaggataa cgataaccag aatctgaatt atcgtatgtg tcattaagat acgcgctgat
    27841 atctgtctgg ctttctgctt gttcttgttc ttttgtgatt tttccgtttt cttccattac
    27901 acttagtact gtgtttttcc gatcattcgc attttttggg tgatcgatcg gattataaat
    27961 accaggacct ttcagcattc ctgccaaagt agcagcctca gacagcgtaa cattcgttgc
    28021 atctactcca aaatactttc tggaagcgtc ttggacaccc cataccccgt tgccaaaata
    28081 ggcattgtta agatacatgg tcaagatttc ttctttgctg tacttctttt cgatctcgat
    28141 tgccaaaaat aattccttcg cttttcgatc aaacgtttgg tctaatgtca gatacgcatt
    28201 tttggcaagc tgctgagtga tcgtacttcc tccgccccct gatgttccgc cagacaagac
    28261 cattcggact gctgcacggg caatcccttt gatatcaaac ccgtgatgct cgtaaaaatt
    28321 cctatcttct gtcgagatta ctgcatcttg gatataggga gaaatcgcgt ttaattctac
    28381 atatgttcct ttttgggcgt acagtgtgcc agcttcttcg tttttcttat cataaatgac
    28441 tgtcgattgg cttaatcctg attttaacgt ttcgacattt gcttgttttg ctaaaataaa
    28501 gaggtagata cttgtcacta agacacagat caaaccaatc agcaaaatca atttgttcac
    28561 atgatatttt ttccagatgc gctttcgcca tcgatggaat tgtccgagat atggtctgat
    28621 ccaacgccaa aaactgatca aagcagcttt tatcttttct aaaatgcttt gaaaatccat
    28681 ttgcttcttc tcctgtctat ttgattcaat cgtcttattt taccatagtg aaagagtgtt
    28741 ttcttcctgc ttaagttgta gttcaatatg tttttttacg ttagtgcaag tagagtaaca
    28801 taagaatgaa cagaaaaatg taagtttttt cttaaatatg ttttaaaaat ggtacaatta
    28861 gtctgcgatt gcactgaaag gagtacactt gtgaaaatca cgatcacttt acctatgaac
    28921 catcctgaaa caacaattcg cgacttgctt gaaaaggaat ggttagttcc tagaaaggtt
    28981 cgacactttt tgcgtatacg taaaaacgtt tggatcaatc aacaacctgc tttgttccat
    29041 catgaagtaa agggcaatga ccagatcaca ttgttattcg aagaaacaga ttataattat
    29101 caagaagttc aattaggaga tgcgaagcac atccgtgttt tatatgaaga tgagcatttg
    29161 attatagtgg ataagcctgc aggaatgaag actcatccaa atgaaccgac agaaaacagt
    29221 accttactca atcatttagc tgcttatctc gacactaaac agcaacgacc ttacgttgtc
    29281 caccgcttgg acaaagaaac aagcggtgcc gttttatttg caaaaaatcc atttgtcttg
    29341 ccgatacttg gaagaatgtt ggaacaaaaa atgatctatc ggagatatca agccatcgta
    29401 tggggagaaa ttgccaaaga tatgaccatc accaataaaa ttggacgaga tcgccatgat
    29461 cggagaaaaa gggtcgtaga tgataaaaat ggtcagagcg ccttgacaca tgtcactgtc
    29521 aacaaagtac tttctgaaca aacacaagtc tattgtgttt tggaaaccgg acgtacacac
    29581 cagatcagag ttcatttagc tcataaggga catccgatcg ttggagaccc tctctaccaa
    29641 aatagaacag ctgaacgact gatgctccat gcattagaac tgcacatgat ccatccattt
    29701 actcaagaaa aaatagtcgg taaagcttta cctggattat gggaatagaa ataaaaaaga
    29761 acagagtctt atcagcagat aatgataaga ctctgttctt tttgtgtaat ttgtctgctt
    29821 acttttgatg ttcgtgttct gctaacagca aactaccgat aatcccactt tcattcggca
    29881 gtccccaacg aacgatatat tcacttactt gaggtgtttc catataacca cccatcaatt
    29941 caacaaattg ctgacggatt ttttgaagga gatgatcctg attcattacc ccaccgccta
    30001 aaataatctt ttcaggtgct aacgtcaacg tatagttgat cagtgcttga gcaatataat
    30061 aagcctgaat agcccaaaca ggatgatctt gcggcagatc ttgccctttg atccctgtgc
    30121 gtgcctcaat agacggtcct gctgctaatc cttctaaaca atctttatga taaggacaag
    30181 taccttcata atggtcatct ggatggcgtt tcacccaaat atgtcccatt tcaggatgtg
    30241 cgatccctga aaaaatttct tcattgatca cgacacctgc accgattcct gttcccacgg
    30301 taaggtaaat acaactattt tttccttgtg ctgctccttg cttcaattcc ccatatgcag
    30361 cagcattgac atccgttgtc caagcagcag gaatattgta gcgttccttg attgtgccaa
    30421 gaaaatcgaa ttgtgcccac ccttttttgg gagttgccaa aacgtatccg taattatctt
    30481 ttgttttgtc gataccaatc gggccgaatg agccaatccc gattcccttc aattcataac
    30541 gatcaaaaaa ttcaaatacc tgatccaatg tttcttctgg tgttgtggta gggatactta
    30601 atttttctaa aatttctcca ccatctgaaa ccgcacacac aaattttgtt ccgcctgctt
    30661 ctattccgcc atacattcgt tttcctcctc gaatccgatt gtccgaatct cataaggttg
    30721 gattattggg acaaccgctt gcttcgttgg ttcttcaagc agattcaata tgacagtttt
    30781 tccattgtct ttagttattt ccaatttttc taaatgacta gacatattga aacctcttac
    30841 tactacttta tcactaaact tgctgcgttt caaagctgta attgcaaacg tttcactttt
    30901 tatcgttaaa tattgttgtt ttgaaatcaa tgttccttca tgatgtttga tttgttgagt
    30961 ggaaaaagga atctgtgctg cgtatgcatg caaataagta gagaattttt cttctggtcc
    31021 atgtagttca atgctgtaat tgaaccgatg ctcacctaga cactgtgctt ctggtgttgg
    31081 aaaatatccc caatcgccta gttctcccac actgcgaagt agcgtcactg cgatttgtcc
    31141 gtcttctgtt acttcatatt cattcaaacc aaagtttccg actgttacac cgtactcttc
    31201 atcatgaata ttgacaaatg cttgctgatg ttgcggattc gtggggtttt cccaactttt
    31261 agacacatgg tttgggcgct tcaccacttc aaaaatacta tctgcttcgt gatgttccac
    31321 atgtaacttt gtaggaaaca agacacgtaa gcgatgatct ttcatctgat tgtcgagagt
    31381 cgtttcaaag tcgatttttt tactatcttt tctgattgtc atgattgttt ttatttgaag
    31441 gattcgtttt tctttggatc gttctgcttt tcgataacga aattccacaa ccatctgctg
    31501 ttctttttct aaacgctcgt ctgcagaaac agggatttcc atttcctgta gaatacttat
    31561 ttttgctatt tccggtgtat ctactaatac ttttacttca gagttttctt tgttactgga
    31621 taggattggc tgatcacaga aaggttgttt aaagatatac tcatttccta tatcgccgat
    31681 atcttcaaaa atatgcagat tattcaactt tcgtttgttt ttgcgatctt ccattgtaat
    31741 ggtaccattt ttttcaatcg tcaaatgtag agggccgttt tcgatgattc gtccactctg
    31801 atgaatcatt gtttcctttt cttctgcttt cgcttcccct tctacaagcg cgaatgtttc
    31861 ccaagaaaat gctggcatgt tttctaatgg taaagtcact ttgatcattc ttgccatata
    31921 tggcacacgg aaacggtctt tcggcaaatc ataaccaaat tggacagttt cttcagataa
    31981 aatagctggt atagtggccc cactttttgt ttctacatga tacttacgtt taggcaaatt
    32041 ttctaactct tgatatagtt gttcaggtat tccttcagcg aatctttttc tttctaattc
    32101 gatggttatt tctgcttcac cagttttatc cattcctgca gtatttacaa tgacaaatgg
    32161 aaagctgtct tttgggaatc cggtcgtatc gattgctgcg gtcagctgtt ctaaggaatc
    32221 ttgtgcaaga tactttccaa cttcatctgc tttttcaaaa cgaggtatca tttcccgatg
    32281 gacagaatcg actgaacaac cgcaaatact gtcgtgagga tgattttgca ttaatgtctt
    32341 ccaagcataa tctaattgat catggggata gtttccacta acttcataag ccatcgtcgc
    32401 aagtggttct gtaatatttt ctagctgtcg ctggaccttg gtattccatt gttttaagta
    32461 aacccgagca gatgctgtat tggctaaagt ataccagcca tccgtttctt gacttgtcaa
    32521 ttctccttct actgagccta agtcttctgg cacatctttt tgaactgctt ccagatagtc
    32581 agtaaaatta gaatgaataa actcgtaatc tggaaacagt tcatttgcga gatggattgc
    32641 ttttgatata tctttttgta ccggttgatg atcaacacca ttcatcatca ataagtggtt
    32701 cgtagaggca tattgttctg catccgctaa tttttgcttc caaaaggcaa gagctgcttc
    32761 tttttctact ggaatctcat tcccgttgct ataccaattg gcaaatagca gaccaaagat
    32821 tgctgtttga tcaggtcctt tccaccacat ctctgagtat tgggaagaat agttttctgc
    32881 ttccaaaact tgattatcaa agccaattgg ttttacacca cggccaaacg ctgctgctga
    32941 aataccagct tgtttcatta attgaggcgt ctgtcccata tttccaaatg tatcgggaaa
    33001 atagcctaac atgacagggg ttccccattt acggctttct tccatgccaa tcagcatatt
    33061 tctcacgttc gattccgagc tgataagaaa atcatcttgc aagatataaa atggtccaat
    33121 tctcaatttt ccttcattga ttgcttgttg gattgcctgt cttttttctg gccttacttg
    33181 aagatagtca tctaaaataa tcgtttgccc atccaaatga aaactgttga aggacggatc
    33241 aacttcaaat agctctagta gatcgtctat caattcaatc agtcgcatgt gatgctgttc
    33301 gtaagccatg taccattcac gatcccagtg gctatgagaa atgatatata ctttcttttt
    33361 catttacgat acctcctact tttctactct aatatcaaag taatccatga ctaattcgca
    33421 aaacatcata tttgcccaag aaaaccattc tctcgtataa ttttcaggat tgttcacatc
    33481 gaatccttcg tgcataaggt gtgttcctgc gtcagttgct acaagtaaat tcaatatccg
    33541 ctctttttca gatttgtctg acgttgtcat tccttccata gctaatgcaa tcggccaaac
    33601 gtatttttct ggcgtatggg aggatccaat tccttttgcg tactttcctt catagaaata
    33661 tggattttct ttactcaaca atgttcttct tgtttgcaga taatcttcct cttgttcaga
    33721 acaatatccc aagtaaggtg cggcgatcag attgggcaca ttactgtcat ccatgactgt
    33781 ggcgtttccc attccatcta cttcatatgc ataaatcgtt tgcccttttt gattaaccgt
    33841 tcgtccgtga ttctgaatcc cctcttggat ctctttcttc aatttctgtg tttgctgtac
    33901 aatttcttta tcatcgagta tcgtttgaaa aatttcttcc acataaccta acacgaccac
    33961 cgcaaacata ttggatggta ccagatatcc ataacggcaa gtatcatcgc taggacggaa
    34021 accagaccat gtcattcctg taaaagctgt ttttgcacct cgcccgtcat tgatcagcgt
    34081 atcttctttt cttgtcgtgt ctcgtacaaa tgtataagga gagtgttcat gatcttgctc
    34141 tgttttgaac acgtccaaga tttttttgat accttcaata aaagattgat tgaattgatc
    34201 agtttttcct gtatttttgt aaagaagata ggccaactgt attgggtagc ataaagaatc
    34261 aatttcatat tttctttccc agatccaatc gttcattttt gtatgatctg tttggtggcc
    34321 agcaccgtta gcttcttcgt tgaaggcatt cgcatatgga tcgatattga tataaaaaaa
    34381 ttgctttttg actaacccgc tgatcatgtc agctaattcc tcatcttctt ttgcaatcac
    34441 taaataagga cgaacttgtg cagtagagtc tcttaaccac atcgctggaa tgtctcccgt
    34501 caataaaaag gtggttccgt cattttgctt tttgactgtt gtcaacagag tattggcaaa
    34561 tgctgctgta aaattctttg cccagtccgc atggatatcc ccacattttt gtgtgatatc
    34621 ttccataaat ttctgcaccg attttggtat atctttataa gccactaata aaaccttctt
    34681 tctatttgat atatctttaa agattatact tcatacagcc aattatgtct acgctttcaa
    34741 aaaatatctt tttatttact ttttcatcca ttatggtatt atttgaatat gatatatcat
    34801 taagaggtgt ttatatgaat caaccattgt accagacaat atttaatgat ttgcaaacgg
    34861 ctattttaac tggtgaatta cctgtagaca gccaactacc aactgaaaaa gaactttcac
    34921 gtacctataa agtaagcaga atcacttcca aacgtgcatt gactgaatta gagcaattag
    34981 gacttattta tcgcgttcgc gggaaaggca gtttcgttaa atccgcttct ccggaacaga
    35041 ttcttgcttc tcctacaaaa acacgacgga ttctgtttct tcttccatat gtcgctgatt
    35101 tgtctgttgg tgattttagc aagggattga accctgtgat gcaagataaa cagattgatg
    35161 tcatgatgac gacgcttgac tttctacaaa ataaaacagc aaaagagcta atcaatgaat
    35221 ttgacggatt gatttattat gctcttgata cagaacaaca tcttgactta ctttttgagc
    35281 tttcattgaa agaattccct gtcgtcatat tagataaaaa aatctatgag ttaccttttc
    35341 ctaccgttct ttccgataat ttccaaggcg gtgcgcttgc tgcctcttat ttgatcgaac
    35401 agggacattc aaaaatcgct tatttgtttg gtacgcagac tcatccacaa tccgttcgac
    35461 agcgttacct tggttacctt gaagcattgg acaaagctgg tttgactttc cacacttcac
    35521 tggatgacaa aagcgcgaca ttagatgaat tgctttctta tatcactaag tatgaagtaa
    35581 ctgcttttgt ttgtgaaaac gatgttgtcg ctatccagac catgaatctc ttgagacagc
    35641 atggttatca tatccctgaa gatttttcaa ttatcggatt tgatgatatc caagcagcag
    35701 ctcttgttga cccgccactg acaaccatcg cacaagattt tgaacagtta ggtaagcttg
    35761 cgggagatgc tttggtcact tggttagaga caaaagatcg accatccgat agtaaatttc
    35821 cagtttcatt catcaaaaga caatcaacaa aggagaaata aaatgaatat cggtcaaata
    35881 gatactagac atgcaacagc gaatcagcca aatttctcta atgggaactg ccagccttat
    35941 actggcgttc cttttgggat gaattatttt gcaccgcaaa ctacggatca aaacggcagc
    36001 tggtggtttc atcctgatga ccgcgttttt caaggctacc gtcttaccca tcaaccaagt
    36061 ccttggatgg gtgatttcag tcatatgctg tttacgccga tcaatggaaa actacaggag
    36121 aacacccttt tccatgctca aagttcttac cgcccagaag aaagtatatt ttgcccgact
    36181 catctgtcga tctgtcaatt acgttatggc atctgttcta cattgatacc tagcatgtat
    36241 ggcggaatct tatctattga ttacagcaaa aatgacagcg gtttactttt atcctttccg
    36301 ggacgccatc agctctttgt tatagatcct tacacagtgg aaggaaaaat cacacatttt
    36361 gctggttcag aagatcctga tttttctttt tattttatat tgagatttga atatccttta
    36421 acaattgatg agttagctgt ttccaacaaa aatagcgact cgatcccgtt gtattttgaa
    36481 cagacaaaaa aacaaactat ccgatttggg acatctttta tcagctcgga acaagcagct
    36541 tataacctgg aactagaaca aaaccaaagc aaagagactt accttgctca cagtcaagaa
    36601 caatggcagt cctattttga tcgtatacag ctcacacacc atgatcaaca acaattgcgg
    36661 acgttttatc ataatttata tcgggtgttt ttatttccac aaacatttta tgaaattgat
    36721 cctagtgggc aaaaaatcca ctatgatacg actagtcgat ctataaaacc tggtgttctc
    36781 tatacaaata atgggttttg ggatacgtat aaaacggttt atcctcttta ttctttgatt
    36841 gctgtagaaa aatacgaaga aatgctggaa ggttttttga acagctatag agaaagtggc
    36901 tatttgccaa aatggctgtc tcctgatgaa cgtggactta tgcctgggac attgatcgat
    36961 gcggtcattg cagatgctgc tgtaaaagat atccgacgag acttgatgcc tgaatttcta
    37021 acggcaatgg aaaaaggtgc tacggttcaa agcaaacatc caaattatgg acgccaagga
    37081 acagaagatt acttgacata tggctatgtt cctaatcact accacgaatc agtcaatcac
    37141 acattagact atgcttatag cgatttttgt attagccaag ttgccaaggt actagaagaa
    37201 gaggaaatga cgaagaaata tcagcagcaa tcattgaact atcaaaatat ctttgactat
    37261 gagtcaggat ttatgcgttc aaaagataaa gatggcaagt tcagagaacc attttcagct
    37321 aatcgttggg gacaagatta tgcagaagga agtgcttggc aaagcagctt tgctgtttat
    37381 cacgattttt caggactgat cggaaaatat ggtggcagtg agagatataa agaaaaactc
    37441 attgagctgt gcaatcagcc cccaacgttt gacccaggag gatatggttt tgagatccac
    37501 gaaatgagtg agatggccgc gatagaattt ggacaattag ctatttcaaa ccagccaagt
    37561 tttcactatc cttacttgtt cagttatata ggacaacctg aaatggcaca gccactgctt
    37621 aaacagttga tgactcagac cttcgacgat tctccaatgg gttaccctgg agatgaggac
    37681 aatggaagta tggccgcttg gtatatcttc aatagtctgg gattttatcc tgtcacgcct
    37741 ggaaccggtg aatatgttat cggtatgcca ttaattgaca aggctgtttt gcttttgtca
    37801 aatggaaagg aattgacgat tgaagcaacg cctaatcggc cgcagcaaca atttatccat
    37861 aaaatcaaac ggatggacaa agaatatacg aaattatatt ttacccatca agatttgctg
    37921 aaaggcggca cgatctctta ccaattaggg atcatccctc accctaagaa atatcaagca
    37981 aaagatttgc ctttttcctt atcaaactat aattaacaag aggttgtgaa aaagctgtcc
    38041 tgcatcaagt aataagaaca gccaagcaaa aatagatcct catatttttc gtttggttgg
    38101 tcttattacc gcagatgcaa gcttttgaac accgtttatt gagaggttgt gaaatcagcg
    38161 ttctgacctg agtattagag agacaaatgg caaaatagtt cctcatattt tgccattttt
    38221 tgagctaata ccgagggtca gctgattgaa caccgtttag tggagaggtc gtgaaaaagc
    38281 tgtttagttt cgagtattaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa
    38341 atttggctta ataccgagaa actggctttt gaactccgtt tatcgagagg tcatgaaaaa
    38401 gctgccttgc ctcaagtaat aagaacagcc caaaattaga tttctagaca attttttata
    38461 agttatttca ttacattggc tttttctttt atgattctcc actgttttct cggtactctt
    38521 taggggatct gccgacgatt ttcttgaata atttagagaa atacgtggtg ttgttgtagc
    38581 caatctcact tgctatctca ttgatgtttt gattactgtg caaaagcagt tgtttcgctt
    38641 tttccatacg taaatggttc aaataagctg aaaaactttt tttcgtttct tttttgaaca
    38701 gctgtcctag atacatgaca tttaaatgca ggttttctga cacttctttg agtgtaagtg
    38761 gttcttgata atgctggtgc aggattacga ttactttttc cacattgtca ctgtactttt
    38821 tctgtctctg atgctttttg atgaatgaca gaagtagctg ctgaagttct tgcaccgttt
    38881 ttgcctgatg gattttttcg attccttgta aatattcctc atcttccaga tcaatcaatt
    38941 cccgttgaat ctccataaac agtaaaaaac tggaatatcg tatatcttcc ggcatcatgg
    39001 ctgctaactg aaactgttca aaaaaatcag tgatcatttt ttgagcgact tccaattgtt
    39061 tattttgcag cacgcgtctg aaacttgaaa aatcgatggt ttgatctttt gtttctgctt
    39121 gtactgcgta aaaaacatgc tcgtctttat ctccataaaa ttgatgcaac tgtagattgt
    39181 ctttcgcttg ttggaatgtt tcagggattt tgtccaaaga atctgtctct tccccgatac
    39241 tgatcagcca ttcctgatca ggaaagtttt cttgaacaaa acaacagaag ccatcaactt
    39301 catctgcctc caacaacaac gtgatcaata cacggtctcc gtaatttcgt tgatagtaaa
    39361 agatttgttg ccgttcttgc aaccatctat tgataagagt atcatgagtt cttgccagtt
    39421 gaatcaatac tatcctatgc tgcttctcac ttatttgtcc tattatttct tcttcactta
    39481 tttcatccaa ttcttcattc aaccattgac tgaataacag ttcttgatag atttcttgtt
    39541 gatttttttg ttcattttgc tgatctaacc gtgttttgat tttcttgagg ctttcaacca
    39601 attcgaactt attcacgggt ttcatcaaat aattcacggc gcctaattgc atacctcctt
    39661 tcaaataatc gaattcttgg tatcctgata aaatcataaa ttcaaaatta tgacgttctt
    39721 tttgagccgc ttcaataaat tccagtccat ttatttctgg cattgtcaca tccgttacta
    39781 ctaaatcaat ctgtcgctgt tcaagtacag ctagtccttc tttggcattc tctgctgttg
    39841 ctactatttg aaagcctaaa ctttgccaat caattatctt ttttaaccca ttcaaaatca
    39901 tgtattcatc atctaccaat aacacttgat acacgctcat ccctccttga atgtaataga
    39961 aacggtcagt ccacctgtct cattattctc aagcgacata taataactgc tgccgaagct
    40021 ggcacgtaaa cgttcattca cgttttgcag accgatagaa ccgtgaagtt ccactttagg
    40081 atgagacagt ttttcttcaa tctgttttag ccgtttttca gtcatccctt ttccattatc
    40141 tttgatgatg atccttaccc gtttgccttc ctgcaagact ttgacactta gcgcattatc
    40201 gaatcgagta aaatcaatac cgtgtttaaa ataattttca actaatggct ggatcacaaa
    40261 tttaggtact tcgatctttt ccaaatcagg atcgatcata aaatgatagg ctactcgatt
    40321 aggataacgc atttgataca aatagacata tttttcgcaa aagtccagtt cttcttttaa
    40381 agtgatcgtt ttctcttgat tcgtattgtt tcgaagcaaa gcggaaaacg catacacaac
    40441 atctgctaat tcttcacttc cttcgctgat tgcatacatt cggatatatt ctaatgtatt
    40501 atataaaaag tgtggattga tctgtgcttg aagcgcccgc atatgtgcgt cttgctgttt
    40561 gatttctaat ttgtagatgt cttctacaaa ctgattgatg ctatccaaca tttcgttgat
    40621 accgattgaa agttccttca gttcatattc cgtgtttgtt atgtcgatcc gcgtctctaa
    40681 attccctgct gctgtttgat ccatggcctg catgacgata gatacttgct gtgaatatcg
    40741 tttgaatgtt ttataaagaa agataagcag tatattgtct aaaataatga taccaatgat
    40801 cagcggggca agattttttc ccgtttcgat ttttacactt tttttatcta gtacagctaa
    40861 tatacgataa tcatcagaca gttcttggtg ttgaacaaaa ttgttttttt ccagatcgtc
    40921 aatcggcaat tgtgaatttt tcttcagact ctctgtgatg atcatttctt gtctttcaaa
    40981 cgtttcttta gctattttat tatcatggaa tgtgtatagt cgatttgtcg ttccagaaat
    41041 catgtataaa gagagtccat tgaatgacgt tagattttct aaaatcatat ctagttcttt
    41101 cttttgaaaa ccaataaaaa tacttcctga ctcagctcct ccttgtgtga taggcaactt
    41161 gatataaaag gcgtctagta attttggtgt cccctttttt atttccccac ctttgttttc
    41221 tctagaagat tcataatact ccggtagttg attcaacacg atatatatcg cacttagttc
    41281 ttcataattt gcgtagagat ctttgatttg atttgggaaa gaaactaatt catcggtact
    41341 gttttgctgt tcgtatacat acatcagata ctgatctatg ggttttgtta agtaggtcgt
    41401 cacattttcg atcttttcgg aattccctac aagctggttt ttgatgatcc tactcaaggt
    41461 tgttttatca ttcagcattc tgcttgtgct ttgaacggca tcacttgtca tgttttctgc
    41521 ctgtttttga ttttgttccc aagtatggat actaaaaata acggtagcag tcatcgttac
    41581 accgatcatg ataaccgcgt atttccgcaa cagacgattt agtaaggttc cttttgaaaa
    41641 tagaatccga aacttattca taactttcca actgtttatc taattcttca cgccaatgtg
    41701 ttgtcatcgt accattccaa accgtcaaaa gaccaatgac accaaacagg atcaatcctt
    41761 tgaattgcca agtcacgcct aaaattatac cgctaccaat aattattttt agaaaagacg
    41821 aaaagctgga aaaaaccgaa ataaaactta gctttagcag attaggaaca gagatcgtat
    41881 attcactatc taaaatcata ctgtattgat aagtagcaaa taggcagagc aaggcaaaaa
    41941 gtaaaataaa atcaatagct aaaaaaatga gccctttgat ttgaacagat aaatataaat
    42001 tgtaactcaa taaagcagta ccagataaaa ataaactaaa tagcagattg cctcttaaaa
    42061 aatttctttt atatatattc cagcctcttt ttattgtgat ttctttatac tcgaatccgt
    42121 acaaataaaa tagttcattg actgttttcc atgccgcacc taatccaaag atgatcccgc
    42181 cggcaaatgt atatatccag aaatacaagg ttagttttat aatgacccag atccggataa
    42241 ataactcttc caaaaccttc cctaacatat cttctcctcc aaacattaag tttatacttt
    42301 ctcattatac aaaaaaaggt tgggcaatca gcaccttgac ctgattatta gagaaacaaa
    42361 cggaaaaata gttcctcata ttttgtcgtt ttgtgagcga ataccgaggg ttagctgatt
    42421 gaacaccgtt taccaagagt ttgtgaaata gccgcttagt ttcgagtagt aagaaaaatt
    42481 ttgtgaatat ggctttccac atttttacat atatttagct taataccgag aaactggctt
    42541 ttgaacaccg tttattcgga acaagaggtt gtgacacgtc ttttgtcaca acccctttgt
    42601 ctttattcat tattattctt gtcggaactc atcatattgt ttttgcattt ctttcaatac
    42661 cttatcatag ccagccttgt ccaaagcgtc tttcaatttc ggtaatgttt catctggatc
    42721 aacagtccct gtattcaatc catctaaata ttgattcatg acattagaaa tattacttaa
    42781 ttctgttttg acactgtcag tgttgaagct gaatcctaaa atcggtgaag tttctgcatc
    42841 agcaatcgat tgatcacgtt tggcaatcat gtcatccgta atagactctt gtgtatatag
    42901 aatcttgttg tttcctgtat tccaagcaga catatgtgta tttggctgat aaccatctag
    42961 caatttgatt ttatcatctg aaccatcgac tttttcccat gcttctccct cgatccccca
    43021 aacaagccca ttcaataatt ctggatcact gttaagcagc cctagaagtt ctactgattt
    43081 ttctttattt tttgaagtat ttgatacgac aaaatttgcc atttgtgctt gagctgtaga
    43141 ttttagtgga acagtaattg cttttgagac aagttcttgt tttgctgcat ttgtcaaaat
    43201 tgtatcgcca taatcatacg gtccttgtgt ttctccacgc attagccaag tatttccttc
    43261 tagtggataa tccttattgc tcgttgcagc gtcacttgga atgagtcctt gtttgtacca
    43321 gctatgcatt gtacgtaaca gtccgatata cgtttcatta tcgtattggt tgatgatttt
    43381 cgaatcatct ccatccagat caatagcaaa tggtaacgta ttccctaaag gataatcaaa
    43441 gttcccttgg actttgaagc cttgtccaat ggcaaaagct gccgtgtttg gttctttttc
    43501 atggaattcc ttcaagactt tttctgcatc agcgtaagat tggattccat caattgaaag
    43561 atcgtattta tctagtaaag ctttgttaaa agtaagtact tgttgagcaa agacatttcc
    43621 attgactggg aaagcatata attttccatt gaccagattt cctttaatat atgcttcatc
    43681 taaatcatta taagcttttt cagcgtactt aggcgctaat tcagtcaaat cagcaaaggc
    43741 tcctttttgg gcattggaaa catagttgtt cgcaaaggcg atatcataat tttccccgga
    43801 agaaatgatg acgttcattt tcttttcgta atcgccccag ccaatgtaat tgatattgac
    43861 ttttacaccg atctcttttt cgattcgttt gtttgctact tccatcaatt gatcgaaatt
    43921 atctggttta tctccgattt gatacatctg caaagtgtcc gaattgctag cttctgcctg
    43981 gtctttagaa ccagaacctc cgcatgctgc taatgttccc aacaacaacg ctgaaacacc
    44041 tgtaaaggct agtttcttcc ataacttcat ttttcttcct cctaatttta tgatttatat
    44101 ttttgtttct ttattctttt actccgccga ttgttagacc tttgacaaaa tgcttctgga
    44161 aaaatggata gctgatcgca ataggcaacg tcgagatgac tacgatcgcc attctggcag
    44221 attctcctgg aactgaggct aatccgcctg tcaattggct tcctgctcct gaattttgtg
    44281 tcagatattg aatattactt tggattttca tcaaaagata ctgtagtgga actagcttat
    44341 catcttggat gtatagtaaa gcattgaacc agtcattcca gtaacccaat gcggcaaata
    44401 aactgattgt cgcaatacct ggaacagcta acggtaaaac gatctgaaca aatattcgca
    44461 gttcgctagc tccatcgatc cgtgccgatt caataatcga atcagagacc gaacgtttaa
    44521 agaatgtgcg catcacaata atattgaagg gactgacagc cattggcaag atcaatgccc
    44581 aaatcgtatc ttttaactga agaagatttg tcataacaag ataattggcc accatccctg
    44641 gtgagaacaa catcgtaatc aaacaaaaaa cggtaaaaaa acgacggaat ggaaagtttg
    44701 accgtgaaat cgcataagca tataaagaag tagccgtgct attgatcagt gtacctaaaa
    44761 cagtaacaag tacagtgacg aaaagcgctt ggaaaatttt atcctgcatc tggtcgaaca
    44821 agtacgtata accaaatata ctgaactttt ctggccaaaa actgtacccg tgagtcgaca
    44881 atatggactc atcggtaaaa gagatggcga taacaaatat aaatggcaaa atacaggaaa
    44941 tagcaaagag tgcaatcatt atattagaga aaaaattagc cgtcttattg aaagagcgga
    45001 cttggactct ctccactttc ttttttttca tttttttcct ccttagaata atgcagaatc
    45061 gctatcaaaa cgtcgggcga tcgtatttgt gatcatcaac aagacaaagc cgacaactga
    45121 ttggtaaagt cctgcagcag ccgtcatacc aaaatctcct gtggatgtca ggccattgta
    45181 gatatacgta tccaaaactt gagtcacatc atataatgca ccggaattcc taggaatgtt
    45241 ataaaacagg ccaaaatcag ctcggaaaat gtttcctacg gctaaaatcg ttaaaatcgt
    45301 cattagtgga ataagttgtg gtatcgtcac atgtttgatc tgctgccatt tgctagctcc
    45361 atcaaccata gcagcttcat aataagtcgg atcgattccc ataacagatg caaagtaaat
    45421 aatgctgtta taaccaatcc ctttccatgt accaatgaac accaagataa acggccaata
    45481 tttcggttca ttataccaat tgacagcttc tcctccatgt ccagtgatcc actgattgaa
    45541 tattcctttg tcaggactta aaaaagcgta aacaaagtaa ctgataatga cccaagatag
    45601 aaaataaggt agtaaggaca tcgtttgata aactttaacg agtcgtttat ttctcaactc
    45661 gctcataatg attgcaaata cgactgagat cagcagattc aaaaggataa atgctacatt
    45721 atataaaatt gtatttttag tgatgagaaa tgcttgatcc gaagaaaata aaaatttgaa
    45781 attttcccaa cccacccaag gactttccct caagctagcc aagaacccat ctgctgaaat
    45841 atgaaaattt ttgaatgcta cgacattcgc cattacaggt atgtaaaaga aaaaaatcat
    45901 ccaaagcata cccggtacag ccatcaggac aagtgcttta taacgccata gattcataaa
    45961 aaaatgattg aacccctttc gttttttcat ctaaaaaccc ctttcgattc attcgcaacg
    46021 taattatagc gctttcaata atgagaataa ataaacaaag tatagggaaa atgaaacaaa
    46081 gtataggaga ggttgtgaaa tcagcgtttt ggcctgagta ttagagaaaa aaacgggaaa
    46141 atagttcctc atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc
    46201 gtttatcaga ggttgtgaat tcagcgtttt ggcctgagta ttagggagat cgtgtcctta
    46261 ctatctaact ctgagtcaca aggaacaatg gggactgtaa caaaagtctt gtcacagtcc
    46321 cttattccga acaaacggtg ttccagaagt agcttctacg gaaataagcc gaaatttcac
    46381 aaaaaagcca tccgaaagtc agacggacaa gtctgctttt cagatggcgc tacaactact
    46441 ctgcttattc ttttagctat ccattcaatt atttttctct cacttgttct agctcttctt
    46501 tggtcatggg tcgatattgc cctttattca aatctttagg cagccaaaat tttcccatac
    46561 ggattctttt taaatataag acttcttttc ctactgcttt gaccattcgc ttaacttggt
    46621 gaaactttcc ttcatgcaaa atcagacgga taattgaagt ttctgtctct tgatctactt
    46681 gatcaatttg gagttttgcc ggcaatgtct gctcatcgcc atcaataatc agtccttctg
    46741 caaataaacg gatatcttct tctgtcatta taccgctaac ctcagctaga tattctttct
    46801 ccacatgttt tttaggtgat agtaactgat gggcaaacac cccgtcattc gttaaaatca
    46861 agaggccctc tgtgtcttta tctaatcggc cgactggaaa aagatcttcc cgataatcct
    46921 ggtcttccaa taaatcgatt acggtttgat catgattatc ctgagtagca gaaatgactc
    46981 ctgcaggttt atgcaaaatg tagtaaaaat ctttttgata cgtaatgatt tctcctaaat
    47041 aagtaaccgc atcttcgaat tcatttactt gcgctttatc gcttttcacc acttgtccat
    47101 tgacttcgat cgttcctttt ttgattaatc ctttgacttc tttgcggctg ccaagtccca
    47161 tatctgctaa aaacttatct aaacgcatgc gtttccccca tttcaaaaga gcatcaatct
    47221 agctcttctg attcacttat tttattttta atttatgtcg taaacgcgct attcctggtc
    47281 ctagtagtct ttctgccaag cggattttca aagctaaata accatacact aaagccccta
    47341 tacctgctac tacaagaatc aacaatagag actggaattt gctatcttcg tttaaaaaag
    47401 caccaaaaat catctttgtg atccacgaag ccaaaagcat aatcaaggta agaataaaga
    47461 tcagcaatgt cctgcgccag acaaaacttg gtttaaagcg ggctacttca tggattctct
    47521 ttaagatcaa ggtacaagac acagcaaatc cgatcattgt cgccaataaa gggccgtata
    47581 cttcaaataa acgaatggct gggtattgaa gaaggagttt cactagaaac ccaataacca
    47641 aataattaat tgccgccttg ttttcaaaca ttccttgcag catgttggaa actagcatat
    47701 atagtcctag gaagagtcct acaaaacttg cttggatcag tacattactt cctagagaat
    47761 ccggtgtata aaataatgta tttagcggat aggcaagtaa cataacacca aatgtcgccg
    47821 gaaacataaa aaatgaaaac aactgtaaat ttccgcttgt caattttgcc agacctactc
    47881 gatctttcaa cgtaacagct tctgtaatca acggtaaccc agtagacgcg atcgatgttg
    47941 ccaatgcaat aactaccatc gtcaacttat ctggattggc actaaaaatc gaaaaaagat
    48001 ccagtaactg tgcattggaa tactcggtgg atgcagacat gatcttcata aaagtgaact
    48061 gatcgactaa tttgaagatc gttacccctg aacccacaat aataaacgga atcgcttctt
    48121 taacggtatc taacaacaat tccttcgttg caatatttat tttgttttcg ctgtagtgaa
    48181 taaacgccga tgtatacgct tgatgtttct tcaaaaaata caaaaggaca agaatacttg
    48241 ctagcatacc gataaatgcc gcgaaagttg attgcgtgac cgccgtcaca tagtcaccat
    48301 ccatgacttt catgatgatg aatgtagcca atagcatata aaaaatacgt gccacctgtt
    48361 ctacaatctg cgacagcgca taaggcatca tttcttgatt gccttgcaaa taccctcgta
    48421 tcacactcat acaaggaaaa accaatacag cgatactcaa agaacgcatc gttggaatca
    48481 gttcttctcc gccaccagaa gcatgagcta gccaaggaga ggcgatgtac atgaacaaag
    48541 caaacaacag gcctaacccg cccatcatct gtaatgcacg gataaataat cgtcgtgaag
    48601 tgccatattc attcaatgaa ttatagcgtg cagtttgttt tgcaatcgca gcaggtattc
    48661 ctgctgtaga gatcagtaga aacaatgcat atatattgta tcccatattg aataatccat
    48721 tggcagcctt agcattttcg cccatccaag cataccaggg aatgatatag acagcgccta
    48781 gcaatcttga aatgatatta ctggcggtca tccaagcaga gcctcgtgcc attttttctt
    48841 gcggtgttaa ttgatctaat gctggttttc tttgattatc catcggtcag attccaactt
    48901 tctacaaaac attactatca ttttaattgg taaaacagaa actggcaatc gattttatat
    48961 aacttttata agaacaattg tttttttgaa ttcggtttcg tttttttggt aaagtaaaga
    49021 aatatacagc cttttatggc taacatttat aggagatatc aatgacacaa gaaataaaaa
    49081 aactacccgt tatattttta ggtcttttct ttatcacagt tggattgaat tggttcttgc
    49141 tccctcacga tatagcttcc gccggagtgg gcgccatcgg acatttagtt gaaacggcct
    49201 tttctatcca acgctcagtt actgtttgga gcatcaatct tatgatgctg cttctagccg
    49261 ctgtcctatt aggaaaatca gtatttttta agactgtagc gggaagtctg ctttttcctg
    49321 ttgttttaga gattgttcca aaagtggaaa tgatctcctc acatttcttt tcactgattt
    49381 caggaagtct tttgttcagt cttggggttt acacattgta cacagtcggg gcctcaaatg
    49441 gcggtataac gataccgccg attattttcc acaaattttt tcggcttcca atgccgcaag
    49501 gacttttact taccaatagt cttatcgtct ttttgaatta ttatgttttt ggattgttgg
    49561 agacactatt cgttctccta tctattacag tcagttcatt gtttatgaaa atactcatcc
    49621 gcttgtcccc tgtgtcaaga aaatttgaat aaagctggat tttgtcatag cggaaacaaa
    49681 cgatatatta aaaagagtgt gctataataa accaagaaat tgaataaagc gagggatcaa
    49741 tcaatgagct tctctttaga agacatccgt catttactat taaaagaaaa tctattaaaa
    49801 gaatttgttt caacaagagg ctggcatctt gatgtaccag aaaaagctga atttcttcag
    49861 ttaagttatg attcaagaaa agctgatgct tctactttgt ttttctgtaa aggaatgaat
    49921 ttcaaagaag aatatttgga ttcagcaatc gaacagggta ttcaatatta tgtatcagaa
    49981 caaccatacg aaaattcagc agtaggaatc atcgtaacgg atatccgtaa agcaatggcg
    50041 cttcttgcta tggcttttta cgactatccg caaaataaat tgaaagtgat cggattcact
    50101 ggaacaaaag gtaaaacaac tgctgcgtac tttacaaaag caatcttgga tcacacaaca
    50161 aataaaaaaa cagcattgct ttctactatg aatacaacgt tagatggcaa aacatatttc
    50221 aaatcccatt tgacaacgcc tgagtcatta gatttatatc gtatgatgtc agaagcagtt
    50281 gaaaatggta tgactcatct agtgatggaa gtttcttcac aagcttacaa aacacaacgc
    50341 gtatacggct tgactttaga tgtcggaatc ttcttgaata tctcgcctga ccatatcagt
    50401 ccgatcgaac atccgacatt tgatgactat ttctattgca agcgacaatt gatcttgaat
    50461 tcaaaaacag ttgttttaaa tcatgaaagt gactattttg atttacttag ggaaacagcc
    50521 gatctattcc agattccaac gatcacttat ggtcgttcgg aagaaagcaa ttatcaagtg
    50581 atccgttcag ataaaggtac tcacggtttt actttatctt ctcatgaaga tcgcttagct
    50641 atctgcgata ctgcttatga tattttgctg gctggaggat tcaaccaaga aaatgcagcc
    50701 agtgcgatca ttgccgcagc actaacaggt gcctccagtc aagatgcaca agacggatta
    50761 aaagaagctc gtgtaccagg ccgaatggat caattgatcc aatccaatgg agcgcatgtc
    50821 tatgtggact atgcacataa ttatctaagc ttgaaaacat tgctgaaatt tgccaaaaat
    50881 gaacatccag atggccgtgt aattgttgta ttaggcagcc caggaaacaa agcaatctcc
    50941 cgtcgacatg attttggaaa agtgctatca gaaacagctg acatcgcctt tttgacggca
    51001 gatgacccag catttgaaga tccgaaaaaa atcgcggaag aaatcaatga agcaattact
    51061 aatccagatt tgatcgttca ttatgaaatg gatcgtcctg aagcgattcg acgcgcttta
    51121 gcagaaagcg ctccgcaaga ttcagtcgtt attgcgggaa aaggcgtgga tccctatcaa
    51181 aaaatcaatg gtgtagatga accttatgaa ggggattatg caatcgtgaa gcggttgatt
    51241 gaagaataat ggtatttata tactagtggt ttcccatttc taagaaaaaa agggactgtg
    51301 attttttgtc acagttcctt ttttcttaga aatcatttta acttttcact agctaattct
    51361 gctgcctttt ctctttcttc atttaataat ttattatcat agaatttgat aaatgggaac
    51421 cagataatga atgcgatgat tgcacaaatg actgctaaca cagcagcttt ccaatcacca
    51481 ccactaccaa caaatgctcc gatcccgact ggtgaaggcc acggcatctg tgcaagcatc
    51541 ggtttgacga tttccagccg aatggcaaaa taagcgagtg tagcagaagc catcggagct
    51601 aagaaaaacg gaaccgctaa atagggattg tagacgattg gcatcccgaa aatgattggt
    51661 tcattgatat tgaataatcc tggtacaagt gaggcttttc ctaacacttt cagctgatca
    51721 gatttcgcga ggaatgcaat aaagatgatc aatcctaaag tcgcacctga tccaccaacg
    51781 gttacataag aattgttgaa ttctcctgcc aacggataat ttgcgccttg tgcgttagct
    51841 gccatattag ctaatacgat cggggtcaag aaagaagtga tgatattcgc tccatggatc
    51901 cctacgatcc acaatgcatg gatcaagaaa taaatcacca tgacaccaag ccatgtattc
    51961 gtcaactgcg taacaaaccc aaatgggata gcaatcattt tgaagatatc cgttcctagt
    52021 gcaactaata atccattgat gatcatcacc acaaacgcaa tgacaaatgt tgggatcaga
    52081 gcagtgaaag aacgagaaac tcctggcgga acaacatctg gcatttttat gacccaattt
    52141 ctcttgacac acatacaata caattcgacc gcaataatcg acataataat agctgtaaag
    52201 attcctgaag ttcctaaacg ttcgacaaaa gagcccatcc taataccatt tactacagtt
    52261 tctgtctcat tgatagaagc aatcaagctg attttaccat cagtcatgat caattcaggg
    52321 atacacataa agaatgcaaa aacagatagt aatgcccccg tgagcggatt aacatttaat
    52381 ttttgttcat ctcgttcaat tgacgttaat tcataagcaa acacaatggc aaagtaaata
    52441 gataaaatcc ccatcgtcat tgtattgacg atcatgtaca agtcactaac tttgaaaaaa
    52501 gttgcattaa aaaatccttc taaaaatggg aacggtaatg gcaaaatatt taaaaccaaa
    52561 aacatcgagc ctacaatcgt gaaaggaata gaagccatcc ccgctgccat gacagcccga
    52621 acgattttaa gttgcgcaat tttccccata ggtcccatca ggtatttctg caaaaaatca
    52681 aacattagtt attctccttt cctaaatata acgctttttc atattgttta atacggatat
    52741 actgttggtc ttggttgagt gaaattttct ttaaacgata ctgtataaat gcactggcaa
    52801 aaataccgct tacaacaaga atgaaaacaa ataactgtcc tccattgtta tttgccataa
    52861 aaggatacaa gaatgtaaat cctgaggtaa acaacactgg aatcactaat aaattaaaga
    52921 cactctggaa caaaaaatac cactttgtcc atggaacgtt cgtttggtgt tttcgataaa
    52981 gctttacttg ttctcctact gcaggcaaaa tagctaaaat cagaatcatc ggtaaactac
    53041 ttcctaaact tttagctgat agaagcagaa tcatccattg catgttcatg aaaaagaaaa
    53101 aggcagtcag gtaacgtacg attagataac gattgaaata catgtttttc aaactaagtt
    53161 gctttttttc taattcgatt ttccttttta ctgtatcctg cacaatgatc cttcctttct
    53221 atttattttt ttgatacaaa taattcattt cctgtgcaac ttccaataat gtcatcgtcg
    53281 tcatcagatg atcttgtgca tggaccatga taatttccat ttctattttt gtcccgctgg
    53341 catattcttt cagaagttga gtttgtgact gatgcgcatg aagaatctct tcatttgctt
    53401 gtcgcaactt ctcttctact tcttcaaaca tctctttacg catcgagcga aatgcttcat
    53461 gtatcagtgt ccgtgcattt ccgctatgga aaatgatatc aaaagaagtc acttgtagtg
    53521 tttcactgtt tattttttca gccatctgtt ttcctcctta ttcgatcagt ttgataaatt
    53581 tactgctgaa attctcaaaa ataggttcag ctaagatttc ctgctgtact tttagctggt
    53641 caactaacct aacgattgct ttagtcacga ccgtaattcc ttcattttcg acataagaag
    53701 gcgaaactaa aaaaacaaaa tgaatttctt gatattgatc ccactgcatc ccagaaggaa
    53761 tcaatgcaac ggctaccttt gtttcgattc caactggaac agcgggatgt gggactgcca
    53821 ctgtatcact gaatatgatt tgtcccatat tttcacgttg ctggatctgc tttcgcattt
    53881 ctcttatata tccttcttct tcatttatgc tcagcttttg cagcaaatca tgaagaattt
    53941 cttccttttt cggttcgtac gtataagtct tgaaaaattt ttcagacaac tgttcggtgt
    54001 aaatctgctt tttttgctgt agagaaaatg ctggtatcgg tctatctgag gaatgatagg
    54061 gtatagattc tccaactacc tttcgtattt tacgaacatc atcttcattc aaaaaaatac
    54121 tgacatgtaa aactggaatt ttgaaaaaca ttgtcgaaag atcaatagaa gaaataatca
    54181 ggtcgattcc ttttaaagtt gtttcattga tctcataata gccttttaca cttaccactg
    54241 taatattttt accgaattca ctgactacgc gattctttaa tagttgggca ctgccgtaac
    54301 ccgttgcaca gataatcaat gcatgtagtt tatgcgcagc tctttctttt tccaatgccg
    54361 ccatgagatg caaagccaaa taggcccatt catcttcatt gatggtatag tttttcaaag
    54421 ccggcatgtt gctgaaatgc ctttttactg cgtcaaagac tttgggattt tctttcttta
    54481 tctcctcagt caatgggttc tcaatgcgaa tacctcggct caatcgaatc atcatcggtg
    54541 acaaatgatc gagtagtcca ttgaaaagct ggagatcctc tactaatgaa aaatgaaatt
    54601 cctgagacaa tcttttcaga actttcgcta attcctttga tagttcctga tcattttgat
    54661 tttctttgtg attcgactta gccatgagat gtaatgtcaa ataagcaatt tcttcttcgg
    54721 gaaacgatac acctgcgatt acctcaatac gttgaacgat ccgctgcgcc acttgatact
    54781 ctactcgctt agaaacttct tgttcgatgc ctaattcctt gatttcaaac ccttctcgga
    54841 gccgtttgat ccccaaagcc aagtgtaaag tcagattctg tatgataaaa tccgaaactt
    54901 ttaacttagc ttctcttgtt tcatcgagaa tgataatgac tagctcttca aaactgatgt
    54961 ctgggaaaaa acggctttgc cctaaatatt ctttcaatga atttccataa tggtttccaa
    55021 aaaaagtatc catgataaaa tgccgtcgat ttctttcttc cccttcgatc catgtccctt
    55081 gaccatgctt agaaacgatt tttagtgaat atggaatcaa tcgctccttg atttcttgca
    55141 tgtcctttgt caaactggaa cgactgatat aaagctcttc tgccaaatca tccataaaaa
    55201 gaatggcatc ttccaaaagc aattggttca atatatactt ttgtcgatct tcggactcat
    55261 aaaatccttg cggttctcta gcatatccag gtactatttc ttgccgactc aaaaacaagt
    55321 caaatgtcag tctttgtaaa atatgcagtt ggtagccata acctggttta gcaatgatct
    55381 tacctccgtt tttttctact aactctttga tccgctgcaa gtaattacgg attgtccgat
    55441 cagataataa caattcagaa gctaaacgtt gactggtgac atactccttc ttgtgatcaa
    55501 tcaaatatct caacagcaat ttttccttct gtttcattat catccactcc tattccatca
    55561 tatgttctct tcgatcacct ctgctaactt ctggatgcct gtagggattg ggacatacgc
    55621 ttggaaaggg atactaacaa caggctttcc aacagattct cctaattttt tgaatttatc
    55681 caaaaacatc gtcgtttgcg ggctgatcag aaataaatca aaatcactat tcttaatcat
    55741 attatcgcct tctgtagctg aaactgcatc tacgagtacc tcttctcttt tttgcttaaa
    55801 gaaatccgtt gtctttgagg ccatcatcga ggatgacatt cctgccgcac aaataatcaa
    55861 tgcttttttc ataaatcaat cactcctttt atttttataa accaagcata tcttgtaagc
    55921 gttttctatt caaaatatat atttccgtag cattgcggaa aaaatgtgta tcatatcttg
    55981 aataataatt tctggcaccg tgatatacta gtgatagaat tttttcagga ggggatttag
    56041 tgagtatcac agtgaatatt ttgtatacaa gtacaaatgg acaagccaga aaatttgcag
    56101 aagaaatggt cgaaagaaac attgtcaaag cgattcgtgc agaagaagga aacgagaaat
    56161 atgactatta ctttccacta gaagatccag aatctctttt actgatcgac cgttggaaag
    56221 atgaagaagc aatcgaaaag catcacaaat ccgaaatgat ggctcaaata aacgaactcc
    56281 gtaaaaagta taaattaaaa atgaaagtcg aacgtttcac tgatttaccg taaaaagata
    56341 cacatggaat gatagcaatt tatcatccat gtgtatcttc actttttcct tattttttat
    56401 tcgatctcat ctaatgattc aaaccgtgtt tttctcttat ttttcggata agtatttcag
    56461 tcaccccaat atacatgatt cctaaacata ccccaaggat attctcaaaa atgcggatcg
    56521 ttactgctcc tggaaccccg aataaactgg cagtgattgc caaagcacca aaagaattaa
    56581 aaactgtttt atatctatag gtagaacaaa cacctaaagc tagaccacct aatataccga
    56641 cccagttaaa aggaataaat tggctgatac caataaataa aacacatcca atcaatgagc
    56701 caatgattcg gtctactgct cgttctttga aaccaatcga catcaatcca tagctagata
    56761 caatagaaga aaatgcaaac cctgcccaca tcaatcgctg aaaagggaca tactctcctg
    56821 caacgatcaa aagactaatg ccaaatgcat agatgagcat ccaaattttt tcctgcgaat
    56881 acatgccttt caaaaaaatc tctttaaaca agcttttgtc tttgttcttt tctcgatgtt
    56941 ttctgtaaag gagaactgaa aaccaacaaa aaaacaaaaa taataagcta cttgtttgtg
    57001 taaagaaatt cttgttcaac gaatctttag gtaacgaata aacaataaag agatatgaaa
    57061 agccgtacaa agaagcattc cccattttgg gatcactagc ggttgccatt aatatgctgc
    57121 ttagtaaaat aaaatgaatc aacatgccca taactgacca tttgatcagc tggacatacg
    57181 gtgctactaa tagtgagaac atgacgattg ctaaacctat gatcgaatgg gaaatcttat
    57241 aaccaaagtc aacaaaacga atactcatca acatacagaa taaaaccaca gcgtatggtt
    57301 tatgcttcga ttcaaataat gttgtaatta taatgataaa acaaatggca aacaatacga
    57361 tgaataatga tcgtaaaaac aatgaacaaa tatacatgca cttttctttt ttagaagctg
    57421 cttctcgtat tttttgtttt aaaataaacg gatctagttg tagagactga taaaaatcca
    57481 cctgttgtcg tcctttcttt ttacttttat tttttacagt catctatgac aggttgtaaa
    57541 tactatggaa catcatatgg cgttgtcatt ttcttgtcaa ctatttttcc tattcatgtt
    57601 tttataaaaa aacatatcgt tttcttgtat gattaaatca ccacaaaata aacaactgag
    57661 agaacgatat gctttatgaa tggttctatc aaaaaaattc tgagatcaat agacaaaaat
    57721 ttgatgatta cagaggtctc ttacgaaacc ttacagaaga aaaaaacatt gatcgttgct
    57781 gcagttctct tccctgctcc atgtgtctgt agaaattgtg gttctgctgt ggtagatgga
    57841 aataggaaat taatggtggt gaaaaacaag aagaaggaaa ccattgtcca ctttgaataa
    57901 tataatcata tgccaatggt tatacgccta aaaaagcagc gctatacctg taaaaactgt
    57961 cgaagccatt ggaacgctca aagttatttt atccgtccaa ggcactcgat ttcaaatcat
    58021 gtcagacata aaatcacttc tttactgact gaaaaagtgt cgttattttt tatttcgaaa
    58081 agttgttagg tatctttgac aaccgttatt cgcacattga aagagttgag aagctattta
    58141 ccaaagtctt ccaaaagaat tctgtcttga gtgttgaggg ttgtgaattt cgttctcacg
    58201 tttccgttga ggataaactg agttttattt ccgcggatgg cgagacagaa aaattaatcg
    58261 agaaaattta cctacacaaa aaattcctcg attaacaaac tatttcctta gctgcactaa
    58321 tcctaaagaa atagaattct tagttacaga catgaacgcc acctactttc agctcaccaa
    58381 acatgttcta ccaaatgcga aattggtgat tgatcgatta cttggttttt ccacgccttt
    58441 caaggaggcg taacctttat tcacgagttg gttgaagctt tccgagacaa aagacccgac
    58501 ttgtttttct ccttattgac aaagagccat taaaactaga gaaaaaatta caactgatcg
    58561 ctgattattt gtagaaagaa aatcaaagta ttacccaata aacttgagtc attaggcaaa
    58621 aaacactgcg aaactcaaga atgattttcg cagtgttttt gtatattttt tattaactag
    58681 tattactaca aaggtttatt tttcacttct caacgcagca caaaattgtc tttacttatt
    58741 tactggttgg gttcaacctt ctctactccg atactaatat ttgtttgtat accccttcta
    58801 aaatcggtag tttccattca attgatgggt actagttgat tttaaaaact atgcttattc
    58861 aattttttat tttagctcat atatttcgtt tctttctaaa ttttatactg atgaaaacaa
    58921 gtagcacacc caatattgac aatataaact ggctaatgat ggtttctcca gtttttggaa
    58981 gatgcttatt tccactgctt gttggattgt ttcctcttat atctgagcta tcagcaactt
    59041 ttctattctc ttttgttaaa aagatcgcca cttcttgttc tttttcgatc ttgaaagtta
    59101 ctggtgtcgc atccaattca taccccgttg gcgcttgagt ttctaccagc tgatatgctc
    59161 ctggtgctaa gccatctatc gccagttttc catctgctcc tgtggttaga cctgactgca
    59221 gaacggttcc attggcatct tgtaactcaa aaacagctcc ttgcaggact tctccgcttt
    59281 ggtcatcgat ctttgtcaac acgacaccgc ctggtgtaag tcgattttct ttcgtcaatt
    59341 ctaccaccgc tgtctgactt ctttcgatct caaactcgat tggtgtcgca tccaattcat
    59401 accccgttgg cgcttgggtt tctaccagct gatacactcc tggtgctaag ccgtcaatcg
    59461 ccagttttcc atctgctcct gtggttagac ctgactgcag aacggttcca ttggcatctt
    59521 gtaactcaaa aacagctcct tgcaggactt ccccgctttg gtcatcgatc tttgtcaaca
    59581 cgacaccgcc tggtgtaagt cggtttgctt tcgtcaattc taccaccgct gtctgacttc
    59641 tttcgatctc aaactcgatc ggtgtcgc
//
LOCUS       DAMYXM010000011        59274 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.011, whole genome shotgun sequence.
ACCESSION   DAMYXM010000011 DAMYXM010000000
VERSION     DAMYXM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.011"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            58..1575
                     /locus_tag="PNS21_000792"
     CDS             58..1575
                     /locus_tag="PNS21_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDI5158524.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            1547..2836
                     /locus_tag="PNS21_000793"
     CDS             1547..2836
                     /locus_tag="PNS21_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI5158525.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            3011..3907
                     /locus_tag="PNS21_000794"
     CDS             3011..3907
                     /locus_tag="PNS21_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDI5158526.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            3921..4784
                     /locus_tag="PNS21_000795"
     CDS             3921..4784
                     /locus_tag="PNS21_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDI5158527.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            4790..5353
                     /locus_tag="PNS21_000796"
     CDS             4790..5353
                     /locus_tag="PNS21_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDI5158528.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            5375..6886
                     /locus_tag="PNS21_000797"
     CDS             5375..6886
                     /locus_tag="PNS21_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDI5158529.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            6898..7857
                     /locus_tag="PNS21_000798"
     CDS             6898..7857
                     /locus_tag="PNS21_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDI5158530.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            7943..9022
                     /locus_tag="PNS21_000799"
     CDS             7943..9022
                     /locus_tag="PNS21_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI5158531.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(9274..10674)
                     /locus_tag="PNS21_000800"
     CDS             complement(9274..10674)
                     /locus_tag="PNS21_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HDI5158532.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            10892..12553
                     /locus_tag="PNS21_000801"
     CDS             10892..12553
                     /locus_tag="PNS21_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDI5158533.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            12510..13208
                     /locus_tag="PNS21_000802"
     CDS             12510..13208
                     /locus_tag="PNS21_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDI5158534.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            13224..14648
                     /gene="araA"
                     /locus_tag="PNS21_000803"
     CDS             13224..14648
                     /gene="araA"
                     /locus_tag="PNS21_000803"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDI5158535.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            14955..16115
                     /locus_tag="PNS21_000804"
     CDS             14955..16115
                     /locus_tag="PNS21_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDI5158536.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            16233..18092
                     /locus_tag="PNS21_000805"
     CDS             16233..18092
                     /locus_tag="PNS21_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDI5158537.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDHSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            18202..18636
                     /locus_tag="PNS21_000806"
     CDS             18202..18636
                     /locus_tag="PNS21_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158538.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(18895..19056)
                     /locus_tag="PNS21_000807"
     CDS             complement(18895..19056)
                     /locus_tag="PNS21_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158539.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(19075..20097)
                     /locus_tag="PNS21_000808"
     CDS             complement(19075..20097)
                     /locus_tag="PNS21_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDI5158540.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            20225..20578
                     /locus_tag="PNS21_000809"
     CDS             20225..20578
                     /locus_tag="PNS21_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDI5158541.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20590..21756
                     /locus_tag="PNS21_000810"
     CDS             20590..21756
                     /locus_tag="PNS21_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDI5158542.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(21848..22468)
                     /locus_tag="PNS21_000811"
     CDS             complement(21848..22468)
                     /locus_tag="PNS21_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDI5158543.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(22660..22956)
                     /locus_tag="PNS21_000812"
     CDS             complement(22660..22956)
                     /locus_tag="PNS21_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDI5158544.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            23160..23810
                     /locus_tag="PNS21_000813"
     CDS             23160..23810
                     /locus_tag="PNS21_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDI5158545.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            23826..25553
                     /locus_tag="PNS21_000814"
     CDS             23826..25553
                     /locus_tag="PNS21_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5158546.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            25550..27442
                     /locus_tag="PNS21_000815"
     CDS             25550..27442
                     /locus_tag="PNS21_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5158547.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            28146..29015
                     /locus_tag="PNS21_000816"
     CDS             28146..29015
                     /locus_tag="PNS21_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HDI5158548.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            29224..30249
                     /locus_tag="PNS21_000817"
     CDS             29224..30249
                     /locus_tag="PNS21_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDI5158549.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            30288..31238
                     /gene="manA"
                     /locus_tag="PNS21_000818"
     CDS             30288..31238
                     /gene="manA"
                     /locus_tag="PNS21_000818"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDI5158550.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            31332..33035
                     /locus_tag="PNS21_000819"
     CDS             31332..33035
                     /locus_tag="PNS21_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDI5158551.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            33037..35070
                     /locus_tag="PNS21_000820"
     CDS             33037..35070
                     /locus_tag="PNS21_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDI5158552.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            35081..35521
                     /locus_tag="PNS21_000821"
     CDS             35081..35521
                     /locus_tag="PNS21_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI5158553.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(35721..36848)
                     /locus_tag="PNS21_000822"
     CDS             complement(35721..36848)
                     /locus_tag="PNS21_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5158554.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(36851..37528)
                     /locus_tag="PNS21_000823"
     CDS             complement(36851..37528)
                     /locus_tag="PNS21_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5158555.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            37792..39558
                     /locus_tag="PNS21_000824"
     CDS             37792..39558
                     /locus_tag="PNS21_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDI5158556.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            39573..40010
                     /locus_tag="PNS21_000825"
     CDS             39573..40010
                     /locus_tag="PNS21_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI5158557.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            40023..41174
                     /locus_tag="PNS21_000826"
     CDS             40023..41174
                     /locus_tag="PNS21_000826"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDI5158558.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(41236..41934)
                     /locus_tag="PNS21_000827"
     CDS             complement(41236..41934)
                     /locus_tag="PNS21_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDI5158559.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(41927..43279)
                     /locus_tag="PNS21_000828"
     CDS             complement(41927..43279)
                     /locus_tag="PNS21_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HDI5158560.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(43502..44902)
                     /locus_tag="PNS21_000829"
     CDS             complement(43502..44902)
                     /locus_tag="PNS21_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDI5158561.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            45063..45800
                     /locus_tag="PNS21_000830"
     CDS             45063..45800
                     /locus_tag="PNS21_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDI5158562.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            45925..46404
                     /locus_tag="PNS21_000831"
     CDS             45925..46404
                     /locus_tag="PNS21_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HDI5158563.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            46401..47168
                     /locus_tag="PNS21_000832"
     CDS             46401..47168
                     /locus_tag="PNS21_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158564.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            47398..47967
                     /gene="lepB"
                     /locus_tag="PNS21_000833"
     CDS             47398..47967
                     /gene="lepB"
                     /locus_tag="PNS21_000833"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDI5158565.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(48071..48673)
                     /locus_tag="PNS21_000834"
     CDS             complement(48071..48673)
                     /locus_tag="PNS21_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDI5158566.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            48779..49248
                     /locus_tag="PNS21_000835"
                     /pseudo
     CDS             48779..49248
                     /locus_tag="PNS21_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            49354..49938
                     /locus_tag="PNS21_000836"
     CDS             49354..49938
                     /locus_tag="PNS21_000836"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDI5158567.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            49941..51014
                     /gene="prfA"
                     /locus_tag="PNS21_000837"
     CDS             49941..51014
                     /gene="prfA"
                     /locus_tag="PNS21_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDI5158568.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            51007..51846
                     /gene="prmC"
                     /locus_tag="PNS21_000838"
     CDS             51007..51846
                     /gene="prmC"
                     /locus_tag="PNS21_000838"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDI5158569.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            51839..52018
                     /locus_tag="PNS21_000839"
     CDS             51839..52018
                     /locus_tag="PNS21_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158570.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            52040..53065
                     /locus_tag="PNS21_000840"
     CDS             52040..53065
                     /locus_tag="PNS21_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDI5158571.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            53240..54484
                     /locus_tag="PNS21_000841"
     CDS             53240..54484
                     /locus_tag="PNS21_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDI5158572.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            54637..55584
                     /locus_tag="PNS21_000842"
     CDS             54637..55584
                     /locus_tag="PNS21_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI5158573.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            55718..56347
                     /gene="upp"
                     /locus_tag="PNS21_000843"
     CDS             55718..56347
                     /gene="upp"
                     /locus_tag="PNS21_000843"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDI5158574.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(56486..56728)
                     /locus_tag="PNS21_000844"
     CDS             complement(56486..56728)
                     /locus_tag="PNS21_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158575.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            56849..56921
                     /locus_tag="PNS21_000845"
     tRNA            56849..56921
                     /locus_tag="PNS21_000845"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56882..56884,aa:Thr,seq:cgt)
     gene            complement(57040..57678)
                     /locus_tag="PNS21_000846"
     CDS             complement(57040..57678)
                     /locus_tag="PNS21_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDI5158576.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(57801..58433)
                     /locus_tag="PNS21_000847"
     CDS             complement(57801..58433)
                     /locus_tag="PNS21_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDI5158577.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            58784..59083
                     /locus_tag="PNS21_000848"
     CDS             58784..59083
                     /locus_tag="PNS21_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDI5158578.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT        19740 a        10320 c        12187 g        17027 t
ORIGIN      
        1 attgacaacg ctttcaacga ggtgtattat tcatgtataa attaaaggag gtcagttatg
       61 gaagccacaa tgttgattag tagtgtcaag cgtgtaggca aagttgatcc gcggttgtac
      121 agttcattta ttgaacatat gggacgagca gtatatgaag ggatttatca gccagatcat
      181 ccatcagcta ataaaagtgg atttcgccaa gacgtcattc acttggtaaa agaattgaac
      241 attcccatga ttagatatcc tggtggaaat tttgtgtcag gatttcgttg ggaagacagt
      301 attgggccca tagcaaatag acctagaagg ttggatttag cttggcggac aattgaaacg
      361 aatgaagttg ggattcatga attttataac tggtgtcagc atgtgggggc cgaaatcaat
      421 atggctgtca atctgggtac tcgtggagta gatgcagctc gtaatttagt cgaatattgt
      481 aattttccta aaggaactta ttggagtgat ttaaggcgtt cgaatggtca agagagccct
      541 tttggaatta agacatggtg tttagggaat gaaatggacg gtccttggca gattggacat
      601 aaaacagcgg aagaatatgg acggttagca gaggaaacgg ctaaggcgat gaaactcgta
      661 gatgattcaa ttgaggtggt tttatgcgga agttcgaatc ggcaaatgcc tacatttggt
      721 gattgggagt taacagttct tgatcaagcg tacgatcaaa tagattattt gtctctgcat
      781 caatattatg gcaaccaaaa aaatgatctt tctcattatc ttgcacgttc cttagacatg
      841 gatcggttca tttctgaagt gacagcgatg tgcgatacag tcaaggcgaa gaaacatagt
      901 aagaagcaga tcaacctgtc ctttgatgaa tggaatattt ggtatcattc agcggaacaa
      961 gatgagcaag cagcgccttg gcagattgct cctcctttac tagaagataa ctataatttt
     1021 gaagatgcat tactattagg ctgtctatta atcactttat tgaaacatgc agatagagta
     1081 aagattgctt gtcttgctca attggtaaat gttattgcac caatcaggac agaaacaaac
     1141 ggagaagctt ggagacagac gatctattat ccatttatgc aagctgctgt gtatggacaa
     1201 ggagaggtgt taagcccaca aattcgttca gcaaactatg cgacggaaga ttttgaagaa
     1261 gtgccgtatt tagaaagtat agccgtaata aatgacaaag aacttgtcat atttgcagtt
     1321 aaccgtggag aagaagaaat gaatctaact gttcacttgt ctgaattatc attagaggga
     1381 gtgattgatt tttctgaaat gagtggattt gatacaaaag caactaatct tcctaaaagt
     1441 gaacaagtaa aaccaagtca ttcaacaaat gtaaaagctg aagatgggaa aatcaattgt
     1501 ttattgaaac cgctatcttg gaatgtggtc agatgtcgtt tatcagatga aggaggaaca
     1561 aaaaatgaaa ggtaaaaaat ggttaattgg ctctttgttg gtagctgcaa caggtatttt
     1621 aggagcttgc ggtggcggaa gttcagcaga atctgattct gataaagaag tgactttgac
     1681 tttttggaat gggtttactg catctgatgg tgaaatattg caagaaatcg ttaatgaatt
     1741 taataaaaca aatgataaaa acatcaaaat tgaaatggat atcatgactt gggccaattt
     1801 gaatgaaaaa cttcctacag cgatttcttc taaaaaagca cctgacttta ttgcattaaa
     1861 ttatccagat tttgcacagt atgtagaaaa cggagcggta caaccattag acgatttctg
     1921 ggaatatgat ggagtagata aatctgattt taaagaaacg gctattgaat taggtgaagt
     1981 agaaggtaaa cagtattttg tccccatgca ggtacaaggc atgtatatgt attggaataa
     2041 tgatttgttt aaacaagctg gattagatac tgaacaacca cctactactt gggaagaatt
     2101 gacagaaatg gcaccaaaat taactgatag tagtaaaaat gtatcaggtt ttgtcttcaa
     2161 taaggatgga actgcaccgt tatataactg gatgttagca aatggcggta aattagtgaa
     2221 cgatgattat actaagtctg aatttgcgtc tcctgagaac cttaaaacgt taaaagcgat
     2281 ccaaaaaatg atccatgtag acaagactgg tccagaatcg atttctggcg ccgaaatgga
     2341 caatttaatg aatgctggtc aactagccat tgaaatcaat gggccatggt taaataacgg
     2401 attgaaagca aacgagattg attataatgt gactacctta ccacaaggga gtgattcaga
     2461 caaaaaaaca gctattttag atggcgtagg atatgccatt ccggcaagta cagataataa
     2521 taaaaaagaa gctgtctatg agtttttgaa atattggaat acaactgaaa ttggtaaaaa
     2581 atggagtatc gaaaatggtt ttcctgccta cttggattca gtagcaaaag atgaagaagt
     2641 aaaaaatgat cctattgtat ctgaattatc gaaacaaatg gactatgcag agccatattt
     2701 accaggtttt acccaaatct ctgcgatcaa taacgatatc atcaatccac tgattgaaaa
     2761 attattagca ggagaagatc ctgagacact catgaacgat gcagatgatg ccatcaatca
     2821 attactgcaa aaatagtaca gaactttata caggatagac tgttggcaag atacagatat
     2881 ctgaatgatc gacctagtat ttgcagatag gtgttattgc aaaaatcaca gcgattgttc
     2941 ggatatctta ttcagattcg tttaaaccaa gattgtatat tttatcaaaa tgaaagatga
     3001 ggtggactat atgaacggat tcaccaaata ttggaatcgt cctgatcgtg ctgcgtattt
     3061 gtttttaact ccatcttttt tagttttatt attatttact gtcataccgc ttttaggaac
     3121 atttgctatt agttttacag atttgaatat tttctttaca tcaagaaatt ttgtaagttt
     3181 tgaaaatttc acacgtatat ttagtgatga acgagcagtt aattcgttga ttcattcttt
     3241 atatttcacc gtactcgaaa cgccaacaca aattatcgtt ggtttattag cagcagtagc
     3301 attatctaaa aataccagat tcaatcgttt ttgtcgttcg acattttaca ttccagttat
     3361 ttgttcattg acgtcaattg cgattatttt ttctatgttg cttgatccga atgtaggggc
     3421 gattccttac ttgttttcac aagtcggctt gccgatccct caattttttc gcgatccaac
     3481 ccttgctatg ccgacggtag ccatcatgac tgtttggaag aattttggtg ttactatgac
     3541 gattctcttg actgctattc agggaatctc tccctctttg tatgaatctg ctcaaatgga
     3601 tggtgcaaca aatcgacagc agttttttaa cattacgcta cctcagatcg ttccctcctt
     3661 aggtttttgt attttaacga atctgattgg ttcgatgcaa gtgtttgacc aagtatatgt
     3721 tgcaaccgct ggtggcccac aatttaagac agaaacagct gttcaatata tttacagtcg
     3781 aggatttaca gcaccttatg aattgggcta tgcatcagca ctgtcttcgg ttttgtttat
     3841 cattgttgca actatagcta tctccactaa tgtgtacatg tcacggaaag aaaaacaaat
     3901 gaaataggag ggaaacctgt atggcaccag aaagctcgat tacagttact gtgaatagtc
     3961 gaaaagcagt tagtggtaaa aaaatcattc gaacgattag tatgctagtt atattgttgg
     4021 ttattgcgtt tccttttttg tggctgatta tttcttcttt taaacacgaa aaagacatta
     4081 tctcatttcc gcctagaata tttgcagata gctataccct agataactat attaaggttt
     4141 ggacaacgat ccctttatta gattacatca aaaatacagt gatttttgct ggagggacag
     4201 tgatcacttc tgtattcttt gattctcttg cggggtatgc atttgctcgt atgcgtttca
     4261 aaggaaaatc agttttgttc tatttcgttt tgctgacaat gatgatccca ttccaagtct
     4321 tcatgattcc tctatttatt gaagtcaatt tgcttggcat gcttgatact tatgcaggtt
     4381 tgatcattcc tcgtatgaca actgcatttg gtatcttcat gatgcgttcc ttttttatta
     4441 ctttaccaga ttctttggaa gaggcagcac gcattgatgg attaagtgag tttaatattt
     4501 ttttgaaaat catgttgcca ttgagcaaac ctactctttt atcattaggg attttcacgc
     4561 tgatgaatag ttggaatgac ttgctttatc cattgatatt gacaagtagt tcaaaaatga
     4621 gaactttacc agcagggtta gctttattta cagggcagaa catttctttt tatggacctg
     4681 taatggctgg aacggttatt tcaatgctgc cattactagt ggtttatatt tttgcacaga
     4741 aatattttgt tcaaggaaca gctatgtctg gaatgaaaga gtgaggaaga tgaaaatagt
     4801 tgagactaaa ctttatggac agttgattgt ggtttctgat tacatcttat taggtattct
     4861 ctgggtaatc gtgtgtttgc ctgttattac tatcgtgcct gctacatgtg ctgtttttta
     4921 tgtaatggcg caatggaaag gtgagggatc aggaaatatc atttattttt tctttcaagg
     4981 aataaagcaa cattttggta agaacttgtt ccttagtctg ttaacgatca ttatttattt
     5041 tcttgttaat caattattag aagaacagac aatcgtactt tcaattagtg gatatgtagt
     5101 tttacttatt tattccatgt ttcttttatc ctggatatat gtgatttctg aaaacaaaga
     5161 tatcacattt ttacaaattt ttgaacaaag tgcatggaaa gtattttttg attttttgag
     5221 aaatattctt tgttgcgtat tgttattagg atttgtactg ctcatatttc ttttccctcc
     5281 ttttattttt atctttgctg gtggcgtatg gaaacttgta tctattatct tgactatagg
     5341 aaatgaaagg tagggaaagc tgttggacga agaagtgaaa gtaaaaatca gagaggattt
     5401 tacggtagga gagatcagtt ctcggctgtt tggctcattt gttgagcata tgggaagtgt
     5461 cgtctataca gggatttatg aaccgggcca tgaaacagct gatggtgacg ggtttagaca
     5521 agatgtcctc tcttatgtaa aggaattaga attaggtgtc attcgctatc ctggaggaaa
     5581 ctttacatct ggttatgatt gggtggatac tgtaggtagg aaagaacaac gaccaaaaaa
     5641 aattgattta gcatggcgag gtatagaacc aaattctttt ggactccacg aattttttaa
     5701 atggatcagt catgtacaag cgaaaccgat attcacggtg aatttaggaa caaaaggaat
     5761 tgatgaagca aaaaatttag tagaatattg taattttcca aaaggaacct tctggagtga
     5821 tttaagaata aaaaatgggc aaaaaaagcc ttttggcgtt aaattatggt gtttagggaa
     5881 tgagctagac ggtccatggc aagtgggggc taagagtgct tatgaatatg gtaggttagc
     5941 aaatgaagcc tcaaaagtca tgaagttagt ggatgattca attgaaacaa tcttagttgg
     6001 aagctctacg ccacgtttac catcgtaccc tgaatgggat cgtatcgcat tagaagaagc
     6061 ttatgagaat gtagattatt tagctttgca taactatatt gataagtatg atgaagaaga
     6121 cttgactaaa cctcctaaaa atgagcgtga tgatacagcg acataccttg caaaagctta
     6181 tcgattcgat cgtcaaattg aagaaatcgt tgctacttgt gattatgtaa aaggatattt
     6241 gagaagtgag aaaacaatgt atctagcgtt tgatgaatgg aatgtacatg cccaaccgga
     6301 aaaaagacat agggattttg aagtcggtag tcccatcgat tggtgttact ttactatgga
     6361 agatacatta ttatttggtt cattagggct ggctatttta cgacacgcag atcgcgtaaa
     6421 gattagttgt caatcattgt tagtaaacac aataccttta atactgacag atgctaaagg
     6481 acaagcatgg aggaatccaa cttattatgt gatgcaacac ttatcgaaat atgctaaagg
     6541 tcaagtattg aaacaacaaa tcatttgccc tgtttatgat tgtgaaaaga taaaaaatgt
     6601 tccttatgtg gacagtgtga tcgtagataa cggcaatcag ctgactattt tcttgatcaa
     6661 tcgcacaaat gaacaacaaa agataacgat tgaacatgat ttcaatttgc tagatcaagg
     6721 agagcactac acattaacca gtagcgatct gttggacaaa aatacacgtg aagaaccaga
     6781 aaaaataaaa cctgaaataa actatttttc aatagaaaaa catgacgaat taaatgtagg
     6841 gatagaaaaa tactcttgga atgtaattag aataaagaaa atgtgaggaa atttgttatg
     6901 atgaagacac tttataataa tcctatcgtt cggcaacggg cagatccttg ggtatataaa
     6961 catacagatg gttattatta ttttacagga tcggttccag gctaccaagt gattgaatta
     7021 agaagggcaa agtcactgaa tgaattagaa catgctgcaa gcctgattgt atggcaggct
     7081 catgaagaag gaccgatgag tgaattgatc tgggcacctg aagtccatta tatcaatgga
     7141 aaatggtata tttactttgc tgcaagcaat cataaaacta tacgtgatga atctcatcat
     7201 caccgcatgt tcgtacttga aaatcagcat atagatccaa tgaatacaga ttggatagaa
     7261 aagggacaaa taaaaacgaa aagggaaact ttcagtttag acggaacaac tttctcttct
     7321 caaggaaagc tatattatgt gtgggcccaa caagatcctg aaattactgg gaattctaat
     7381 ctttatctat ccgaaatgga taatccttgg acgttaaagg ggaagcagat cctcctttct
     7441 atacctgagt atgaatggga aaaacggggt ttttcagtaa atgaaggtcc agcagtcctt
     7501 actcgcaatg ggaaaatatt cattacgtat tctggtagtg caacagatga aaactatgcc
     7561 atgggcctgc tgtgggctga tgagcacaag aatcttttat taaaagaatc atggcataaa
     7621 ttatcggagc cagtattcgt ttcttctgaa aaaaataagc aatttggtcc tggtcacaac
     7681 tcgtttacgg tttcagaaga cgggtgcttt gatattttga tctatcatgc tcgaccagag
     7741 aagaaccaaa aaggggatcc gttagacaat cctaatcggc acgcgaacgc gcaatgcttt
     7801 acgtggaatg agcaaggatt tccagaattt ggtgaacctg ttccgtatgc cttatgagca
     7861 caagaaacct tgatttttgc ttgaataaac cttgcagact cgttatgata tatgtacgta
     7921 caaataaaaa ggggcaaggc aaatggcaaa atatcaagat attgcagaag agataaaaca
     7981 aaagatcctt cgtgaggaat acgaagtaaa tacaaccatt ccctcagaat tcaagctgca
     8041 agaaatatat gacgtcagca gacatacgat tcggcaagca atcgctgtgt tggtaaatga
     8101 gggctatctg cgtaaagaaa aagggtcggg cacatatgta gatgacagct ataagaaaga
     8161 aaaaacacca ttgaacagaa caaaaacgat cggtgtcatc accacatatc tctctgatta
     8221 tatttttccg tcgattatcc gaggaatcga aaatgaatta cgagaatctg ggtattcatt
     8281 gcttttagcc agtacgaata acgatcacga attggagaaa gagtgtttga agaaaatgat
     8341 tgcttatggg gtagatggtt tgatcgttga gcctacgaaa agtaatcagt ataacccaaa
     8401 cttagcactt tatgttgctt tgagagaaca aggcattccg atggtgatga tcaatgctgc
     8461 ttatgaagag ttaaatgcct cttatatttg tttagatgat gtgaaagtcg gttttatggc
     8521 agcagaatat ttgataaaaa aaggacatca gcgtctgttg ttcatcacaa aaatcgacga
     8581 tcttcaagga aaatacagga tgaaaggttt tatcagcgcg tgtgaacagt ataatgtgcg
     8641 ttttttgacg gaagatatca ttactttcac cacggaaaca cgagaaacaa tcgctgaaaa
     8701 aacagtacaa cggttgaaag aagaacaagc tttagatggc atcatttgct ataacgatca
     8761 aatagctagt ttaatgctag ataagttgac agaaaatggc tatcatattc cagatgacct
     8821 atcgatcgta ggaacagacg attcttcttt aagccattta gggaataaaa aattgacaac
     8881 attgacgcat ccaaaagaaa agttgggaca agatgccgca cggtggatcg tcacaacgat
     8941 tgaaaaagga caggcacagc caaatattct ctatgaacct gtactagttg aacgggaaac
     9001 agtgaaagaa aacgaaaaat aagagacggg tttttaccgc ctcttattgc tcctacggtt
     9061 actcgtcgca atgtttgtga gagcttttgt catcgctcaa ggctgaacgt ttctttcacc
     9121 acctctccac taaacggtgt tctcctacca actggtcaca agtcacaata ttcatcaaac
     9181 atgagaagct gttttttgaa attgttcctt gtgactggaa actaagcggt tccttcttat
     9241 ttcttgaagc tgaccacttc tgtcacaacc tctttaagaa tgatggatag aaatacgttt
     9301 aggataagtt tttttagatg cactttgttc gatttcctct aaagatttcc cacgagtttc
     9361 tggtacgatc tcttgaataa ataaaacccc taaaaagcag atgatcccaa aaatgccgaa
     9421 aacgatttct tgagacatag aagcagtcat cacaggaaaa agcaagccaa ctaggaaaga
     9481 accaatccaa ttaaaagaag aggctaggcc agatgctcgc ccgcggacag ctagtgggaa
     9541 tatttcgcct acgattaccc atgtcagtgg agcccatgtg caagaataaa atgcgacata
     9601 aatacataaa aacaagagaa tcaacaacga atttgtatga gcatccagta cagttcctaa
     9661 aacagctggt aggatgaaag aaagtcccat cacgctaccg cctattttca ataaagttcg
     9721 ccgattgaac ttgtctgcaa taaccaaaaa gagcaaagaa ccagcaacta aaatgattcc
     9781 ttggatgatc ggccacatca atgcatcgct cgctgcatgc ccagtcgctt tttctacgat
     9841 cagcggaata tagtaaaaaa tagcattagc tccttgaaat tgctggaata ctgctacacc
     9901 aataccggca atgactaagg aacgatattt ttctaaaaac aaagtatgcc aagaagttcc
     9961 ggttgtcttt tcttgtttga cattcagctg gatttgttcg aattcctgtt cagcttcttt
    10021 tggtgtccga atatagttca atactctttt tgcctcttcc aagcggccag attgcataag
    10081 aaaacgcggt gactctggta gagctaaaac accgaaaaat aaaatcagcg cggggacagc
    10141 agcgagccct aacatcacgc gccaagccat cgtttcaggc aaccctttta gcaaataatc
    10201 tgcaacatag gaaaggagca tgccagaagc aatcatcact tgattgatcc ctgacaatct
    10261 tccccttagt ctagcaggag ccatttcaga catataagca ggaactaggg cagatgcagc
    10321 gccaacagct aaacctagaa gaatccttgc tgcaatcaga aaataagaac cattgtgagg
    10381 ggcagatgca gaaaggacag aaccagccat aaaaataagt gcagaaagaa gaatcatttt
    10441 tcgtcgtcca agcttatcag aaagaagacc agataaagag ccgccaagga tcgcacctaa
    10501 catgaccgaa gacgtgatcc atcctatcaa agaagcttta cctgccaaac cccaatcatg
    10561 ctgtaaaaaa ggtaaggcgc cggtcataac accaatatca tatccaaata gaatgccgcc
    10621 aaatgcgcca aaaaaataaa taaacgatga ggaaactttt ttttcttttt tcataagtat
    10681 tctctccttc tagaatttcg agaaggcggt tgatcagatt ttgtaagcaa tttcatttta
    10741 taacttatgt acgtacaaaa caagtgtttt ttattgtatt cttgtaaata actgcttgac
    10801 atgtacgtac aaaagtatac aataatcatt gtaagcgcta ctaaaaagac ggtttgatca
    10861 ggtttgacga gaaggaggga gttaaatcat tatgaaggaa gtaaatgcgc aaagtatgtt
    10921 agatgtcata gatggtcgta catctttagg gattgagttt ggatcaacta ggatcaaagc
    10981 tgtattgatt gattcatctt ttactcctat tgccgctggt agttatgagt gggaaaacaa
    11041 actagaaaat ggtatctgga cctattcatt agaagatatt tggaaaggac tgcgcgtcag
    11101 ttaccgaaaa atggcagcag aagtattcga aatctatggc gagaatctta cgacaatcgg
    11161 gtcgattggg ttcagtgcaa tgatgcacgg ttatctagca tttgatagca aggaacagtt
    11221 actagtacca tttcgtactt ggcgaaatac aatcactgaa gaagcagaac gaatattaac
    11281 ggaaaaattt caatacaata ttccgcagag atggagtatc gctcatttgt atcaatctat
    11341 tttgaataaa gaagaacatg tgaaagaaat taattttttg acaacgcttg caggttatgt
    11401 ccattggcag cttactggtg agaaagtact aggcgtagga gatgcatcag gcatgtttcc
    11461 tatcgatcct tcaacgaaaa cctaccatca agaaatgaaa aatatttttg gtcagttaat
    11521 cgaaaaagcg cacgtagcga ttgacttgga tcacattttg cctaaagttc tagtagcagg
    11581 agaagaagct ggcaagttaa gcgaatcagg ggcttatctg atcgacccta cgggaaattt
    11641 gagaccagga atcccggttt gtccgccaga aggggatgca ggaaccggta tggtcgctac
    11701 gaacagtgta gccaaaggga cagggaacat ttcggctggt acttccgcct tcgctatgat
    11761 cgtattagaa aaagaactag aaaatgtcca tccggaaatc gacatggtca ctacaccttc
    11821 aggagaacta gtaggcatgg ttcatacgaa taactgctct tcggatatca atgcttgggt
    11881 cagtattttt gaagaattta ccaattcttt gggtatggag ataagcagag ataaattatt
    11941 cacggtatta ttcaacaaag cgctagaagg cgatacggat tgcgggggat tactttctta
    12001 tggttatttt tccggtgaaa acattacagg agtaaatgaa ggaaggccat tatttgtacg
    12061 tacaccaggt agccgttttg atttagcgaa tttcatgcgg atccatttag ccagtgcttt
    12121 tggtgcaatg agaatcggaa tggatatatt gaaatctgag aatgtccaaa ttgatcgttt
    12181 agtggggcat ggggggattt ttaaaacacc agaagttgga cagcggattc tagcatctgc
    12241 tatggaggca ccagttacag tgatggacac agccggagaa ggtggagcgt ggggaatcgc
    12301 gttattagca gcctatatga tggatcgaca tgggaagtct ttagaaaact tcttggcaga
    12361 agacgtgttt ggacaagacc aaggaatcac catcacacct tcagcagaag aaatagcagg
    12421 ctatcagata tttatgagac gttaccgtga ggggatcgac attgaaaaaa cagcgattca
    12481 tcaattgcaa gaaaaaggag tgatagaaaa tgctggaaca actgaaagaa gaagtatacc
    12541 aagcaaatct tgacttgcct aaacacggac tcgtcaaata tacatgggga aatgtgagcg
    12601 cttttgatcc tgaaacacga tatttcgtta tcaaacctag cggagtcagt tatgaagaga
    12661 tgacagcaga cgacatggtc gttgtggact tggacaatca tatcattgaa ggtaaactca
    12721 atccctcttc ggatacgcct actcatgctg ttttatatcg ttcatttcct caaatcggag
    12781 gaatcgtcca tactcactcg acatgggcga cgatctgggc acaagcagga ttggatgttc
    12841 ctgcgatggg gacgacgcat gcagatactt tttatggatc ggtaccttgt gctcgatttt
    12901 tgacacaaca ggaaatcgac agcgggtacg aatatgaaac aggaaaagtt atcatagaaa
    12961 cattcaaaga acgcaaaatt gatcccttag caattccagg ggtactttta cacggacacg
    13021 gtccatttac ttgggggaaa gatgcgcaaa gtgcggtaat gaacgcagtc gtactagatg
    13081 aagtatgcaa gatgaattta tttaccagac agctcaattc attttcagaa gagctgcctc
    13141 aacggatttt agataaacat tatttacgaa aacacggaaa aaatgcgtat tacggacaaa
    13201 acaaataaaa ggagtggcta attatgttga atattggaga aaaagaattt tggtttgttg
    13261 taggatcaca gcatttatat ggggaagaag cactgcgaga agtaaagaaa catgcacaag
    13321 aaatggtcga tgaactgaat gaaaatggcc aattgcctta tccaatccgc cttcaagaat
    13381 tagcagttac tgctgatacg atcacaaaaa tcatgaaaga ggtcaattat cgagaagaag
    13441 tagcaggtgt gatcacttgg atgcatactt tctcaccagc aaaaatgtgg atccgcggga
    13501 caaagcttct acaaaaacca ttgcttcatt tagctacgca atataacgaa agcattcctt
    13561 ggaaaacgat cgatatggat ttcatgaatt taaatcaatc cgctcatggt gaccgcgaat
    13621 atgggttcat caatgctcga ttaaataaac aaaataaaat cgtcgttggc tactggaaac
    13681 gacctgagat ccaaaaagag attgctgatt ggatggatgt tgcagtagcc tataatgaaa
    13741 gttttggaat aaaagtggct cgttttggtg acaacatgag aaacgtgggt gtcacagaag
    13801 gagacaaagt ggaagcacag attcaatttg gttggactgt tgattatttt ggtatcggag
    13861 atcttgtaca agtaatcgat cgcgtatcag acgaagaagt cgatcaatta tttgaagagt
    13921 acaaggaatt gtataccttc gactatggtg attatgaaga aaagacatgg gaggaacatg
    13981 taaaagtaca agctcagcag gaaatcggta ttcgccgttt cttagaagaa ggaggctata
    14041 acgcttttac gactaatttt gaagacctgt atggaatgaa acaactacct ggactagctg
    14101 tacaacgttt gatggcagaa ggatacggtt ttgctgggga aggcgactgg aaaactgcag
    14161 cgattgatcg gttgctgaaa atcatggctc gagggaaaga tacaggattc atggaagatt
    14221 atacttacga gctagctagt ggccaagaag caatcttgga atcccatatg atggaagtag
    14281 atccgacgct tgctgcgacg aaaccaagaa tcgttgtttc tcctttatca atgggagata
    14341 gagaagatcc ggcgcgtctt gtatttgatg ggaaagcagg agaaggggta gtcgtatcga
    14401 tggcagattt cggtacgcat tataaactgc tgatcaatga agtagaagcc tttgaaccaa
    14461 cgactgaagc acctaattta cctgttgcac gtgtgttatg gaaaactaaa ccgaatttcc
    14521 atgaaggtgt ccattcatgg attcaggcag gtggcggtca tcataccgtt gtctcgctaa
    14581 atctaacaac agatcaaatt gaaacgtggg caaaattagt tgagttggaa acagtggtta
    14641 ttagataaaa agaggttgtg aaaaagccac ttagttttaa gaaataagaa gagattgacg
    14701 aaatttgctt ttcaactttt ttgaaattta gtcttattat caatgtcagc taacggaaca
    14761 ccatttatca agatatctca ccctcaatat tttgctctcg aaaataaggc ttatggcagt
    14821 aacttgtcat agccttattt ttatcactca gcctactctg ctctaactgc tctttttgct
    14881 tctaggaagt caaatggtaa acagttgtct aattgcagaa atttcgagat acttgttagt
    14941 aaggaggggg ttttatgtat cacgttcatt tgattgatgc tattccagaa caagagatag
    15001 aaaagttaca aggggaatat ttcaaaataa tagatgattt gccagccgaa ggaatagtta
    15061 ttcgtagtac gaaagtgaaa gatgaatggc ttacaccaga tttgctagca atttcaagag
    15121 ctggtgttgg ggtgaacacg atcaacgttg aaaaagcttc tgaaaacggg acgatcgtca
    15181 tgaatacacc aggtgtaaat gctaatgcag taaaagagct tgttttgtgc tgtttgctct
    15241 tatcttcccg tccgataatc gaagcaagtc gtatggtaca aacactaaca ggtcctaata
    15301 ttttagaaca ggcagaaaac aaaagaagtg cttatgtggg acgagaattg caaggcaaaa
    15361 ccatcggtct tcttggtctt ggagcgatag gcacaaaagt agcgctaagt tgctatagct
    15421 tagggatgga tgtcttgggt tattctatcc gcgatgccca gttggattat gttcggcaag
    15481 cagatttaga aacagttctt tctacctcgg attacatcgt agtcatgctg ccgttgacag
    15541 aagatacaaa aggattgatc gatcaggcga atatagaaaa aatgaaaaaa gatgctgtac
    15601 ttatcaatgt aggaagaagt gaaattgttg ataaatatgc tgttatgcaa gcattggaaa
    15661 aacaacatct ggctaaatac ttgactgatt ttccagaaga agagttctta gaaaatgacc
    15721 gtatacttat gttgcctcat ctagggggat cgactcagga agctttggca gacagcggac
    15781 gtttggcagt tgaagcctta aaagattatc ttttgttcgg tacggttaga gaagcggtca
    15841 attatccatc cgcacgaatg tttttccaag cgccgtttcg attcactata ttttatcaaa
    15901 agaaaaccaa tatattagca gagatatttt ctctgctgaa tgagaacgag ctagcgatcg
    15961 cagatatatc gcgtaaccat aagaatgagc atgtctacat attgattgac atagacagta
    16021 ctgattttga acagctcaat caagtgaaac aacagctaga aaaaatttct ggagtacgaa
    16081 aggtccgctt attaaagaaa ccagaaaggc cataaagaaa gagaatattt ggatttcatt
    16141 aattcttctt tccgttcttt actttcgaat ttcgtacaac taaaataaga gaagcaacat
    16201 tcttttattg atagaaatga aggagcaagc atttgaaaag atatataagc agactttcga
    16261 ccaaacagaa aaagatttta aaaggaacag cagcaatcat cagtatcttt tttcttgttg
    16321 tttttagtaa tttatttttg cagtggtgtc aaaatgatct atccgtagac cttgcgttga
    16381 aatttgcttt ttcttggcat acggaaaaat tcttcttagc ctgtctcgtc ttgctgatta
    16441 ttcttatttt tctgattgca ctagcaggat ctgtcccttt aggttccctt acgtatgtcg
    16501 tagccatcgg tgtattaggt tttgcaaatt atatgaaaat gagttatcgg caagaaccga
    16561 tttatccaga cgatttgaag atgatcactg aaatcggatt gctgaaagat atgaccggca
    16621 ctatgctgtt tacagtgatc ttagctgccg caggaaccgt gataggattg ttttgctggt
    16681 acatgtttcg aagcctgaaa aaaggaaggc gattccaatt gatccgatta acgacactgc
    16741 ttgtggcaat tggactgcta gggtatatca gtaatttcaa caatcccgat aatttgttgc
    16801 gaaaagcata taataaaacg gcactatgga taccttacag ccagaaaatg aactactata
    16861 acactggttt tatcggtggg tttttgtaca atctgaaggt agaaccaatg gatgaacctg
    16921 aaggatattc gaaagaaaag atcaaagaaa tcactgaaaa ataccagaag ctagccgatg
    16981 aaaaaaataa aactgtagag gaagagtcgc cgaacatcgt gttcgtgatg agtgaaagtt
    17041 tctcagatca tagtcgatta aacggcgtag aagtatctgg tgagccattg gcagattatt
    17101 atgaagtagc tgatcaaaca tatagtggca atatgctttc tcaaaactat ggcggtggaa
    17161 cagcaaatat cgaatttgaa gcattgactg gtttttctat ggcgttattt aatgcgcaac
    17221 tcaccacccc ttacacgatg cttgtaccaa aaatggatca gcttccttct atcgtttcag
    17281 cgttaaacgc acaatcatac cagacgacag cgatccatcc atacaatact tcgatgtata
    17341 aaagagagga tgtttatcag acattaggat ttgaccagtt catcagcgag cgtacgatga
    17401 cctatacgga tacgatagag aataatccat atatttctga tgagtcagct tacaaagaag
    17461 taatgacact tctcaaggaa gaaaaaacac ctcagttcat tcatttagtc acgatgcaga
    17521 cacatatgcc ctacgatgga aagtatgata atctagacta tcctgcaaaa acttcagatg
    17581 gaagtgggac acttgactta gagaattatc tgcaagatat ttcctatagc agcacggcac
    17641 tgaagcagtt cacggaagaa ttgaagaatc tttctagaag gactttagtt gttttttggg
    17701 gggatcatct tccagggatc tattcagata cgatccaagc aaaaaatgac aaacaaacat
    17761 tgcatgaaac acaatttctt atgttcgaca gtaaaggaga attagaaaag caaacgactc
    17821 acgatgcaat caccagtccg ttttattttg cagcgaatct aatggaacag acgaatcaga
    17881 caacaaatgg cttttatcag ctgcttttat cattagaaca ggaacttccg gcgtttgaac
    17941 gagaactgta ctatcaaaac ggacagtggt ataaagaagc gcaattcaat cgctcgcaac
    18001 aagagattta cgacgaatat caattgatcc aatatgacat tgtcgcaggg aaacaatata
    18061 gtttagcaga tggtttcttt gagcacgaat aagaatggcg agctgtatag aaaccattaa
    18121 attttttcaa gtacttgtca tttgaggata aaaacaagta gaataaataa taacaacaat
    18181 ggagaaagta ggaaaacgaa catgttaccg aatattggct attatgtgca gtccatcgat
    18241 caaatcgtca aggaaacaga agcaatcgga gaaggaatgc atccgaaata tgaagtgatc
    18301 agaaacgcaa ttgatgaagg aaaaacaagt gaactggaca aatcgactct tgaagagatc
    18361 atcctgttat ttgaagaagg aacaaaaaaa tatcgggcaa tgcttatgaa agtcagtact
    18421 ttgcgtccac cagcaaaagc aatgggcatt cataaaaatt ttgaaaaagc ttatctttca
    18481 tatgtagcag gctgtgaaga aatgatccaa agtatcgatc ttgaatcagg ggtagatgaa
    18541 gatctattca atacatctga agcaaaacaa gaccaagcga cagaccaatt gacagctgcc
    18601 atccaaaaaa tgactaattt gttgttgaaa aaataaaaga aatctttgat aacaataaaa
    18661 actagagtta tctaatataa atgtaatatt acctttatga tagagtgatc aaaatccttt
    18721 atgataatga catacctata accaacaaca accttgtact tactcaaaat cgagtaaata
    18781 ctccttttcc gtcgagtcta accatctcgg cggtttttta tttgtagaaa agaacatcag
    18841 gatagacgga aaagtcttgt cctgatgttc ttttcagata gtatggaaag ctgattagtt
    18901 ttctacattc tgtacatatt gttcaaagta gttctgtaaa tcggctttga gtttctgata
    18961 ttcctcttcg ctatactctt tatcttcgat tttcccatca aagaaaggaa tatttagttc
    19021 ttcccgtagt ctgtctaata cgtcttgctg attcatttca ttctcctttt cttactaacg
    19081 atcaaaataa acacagacac attctggtac atagacatct tttttcagta attccagtaa
    19141 tcctgtttca ggatgacggc gatataatgt cagattatcg gtgttctgat tggcacacac
    19201 gacaaaatta ttgcttggat ctagtgcgaa atcgcgggga aaatcccctt ctgttgagat
    19261 gatttgcttg ttttgaatgt gggaaccatc ttcaaaaact gcgaatacag caatcgagtt
    19321 atgtcctcgg ttagaagcat aaaggaactt gccatcattt gaaatgcgga tagctgcccc
    19381 tccattttca ccgtcgaatt cttctggcaa ggtcgatatt ttctgtaatt cttcaaaagc
    19441 acctgtttct ttattataag aaagcacgcg gactgtgctg tctaattcac caaataaata
    19501 agcgaattgt ccatttggat gaaagacaag atggcgaggg cctgttcctg gctctgcagt
    19561 gaaggtagtt acaagattca atttgccgtt ttcggacaca tcataagtat acacacgatc
    19621 tgtaccaaga tcacatacga ctaatcgttg gtcaggagtc agatccgtat agtggacatg
    19681 cgcatgatct tggtttttat gaggacctgt cgcttcttga tggaatattg aatctgctgc
    19741 tttcagttcg ccatctccta atatatggta gacatttact tcacctttat gatagttcgc
    19801 cccgtatagt aattgacgtg cttcatcaac cgctacataa cacaaaggag cgccagactc
    19861 tgttaccgca ttcaaaaaat gaaaatcctt atcgtacgca ccggcaccac ctaatccatc
    19921 cacactagta actgtgtaaa gattgccagc tttacttttt gctacatagg tagggctatt
    19981 ttcttcaaca gctaatgaaa ggttggctaa ttcttctttt tctgtatcaa ggtcgatcgt
    20041 atagattcct ttactttcgc gacgcgtata ggttcctaaa attatctttt cgatcatatg
    20101 agctgattcc tcctaattgt aatgaaatag ttttcgtttt tattctatca cataattatt
    20161 ccacacggct aaagaatgag aatgtgttat actgtttttg aaagcaatta aaggagagag
    20221 tgcaatgatt caggcaaaga ttacagaaat tgggacagaa gcaataaaca aggaagaacc
    20281 catgctgatt ttgttcgata aaacagcaac agctgcctta cgaaaatatt cggtgattca
    20341 agaagtatca aaagacgaac ctttttcttt gaaagcaggc ggaacaattg cttttgatga
    20401 tcaggtatat acgatcgatt atgtcggtcc gatggccaat gggaatttaa agacagtagc
    20461 tcacgtatca ttgatttttg atgcttgtcc agaagaagga caaattgcaa atggaatcta
    20521 tcttcatccg catagactgc ctgctattgg tttgggaagc caaatttttt ataactaaca
    20581 ggaggaatta tggaaggaaa aaataagaag aacagaccgg tatcgtggtt ttggcgctgg
    20641 tttttaaata atcaagtagt cacagcctta cttgttgtat tgttaatatt gctgatcgtc
    20701 tttttattta caaaagtttc atatttgttt gcaccaatct ggcaattttt agcaatcgta
    20761 ggtctgccta tcattttagc aggaatcttg tattacttga tgaatccggt agttgattac
    20821 tttgaaaagc gaaaagtacc aaggctttac agtatcattg ggctgtttgt tttggttgtt
    20881 gcactaatcg catgggggag cgtagtgatc atccctaaga ttcaggaaca aacagtgagc
    20941 ttcatcagta acttccctca atatgtagat acgattgata ataaattaac cgagattttg
    21001 cgggacccgc tgttcaacca atttcgcgaa cagttggaaa cagcaggtga taagttcatg
    21061 aattcggcag gcgatatgat ccaagatata tctaagagta cagttcagag tcttggaagt
    21121 tttgtcggcg cggtagcgac tattttagtg gctctgctga cgatgccgtt tatcttattt
    21181 tatctattga aagacggtaa acaattagca ccttattttg tcaaattttt accgacacgc
    21241 atgcaaaaac cgacgttgaa tgtgctgagt gaaatgaata gtcaagtttc ttcttatata
    21301 agaggacaac taactgtggc ttttgctgtg gcaatcatgt ttatgattgg ttttgcggtc
    21361 attggtttag agtatgctgt cacgttgggg attattgcgg gttttttaaa cctgatccct
    21421 tacttaggat catttttagc gatgattccg gctatctttt tagccattgt agcaggtcca
    21481 ttcatgatca tcaaagtact gatcgttttt gcagtagagc agacaataga aggacgcttg
    21541 atctctccat tagtactagg gaatcagtta gcaatccatc cagtaacgat tttattagtt
    21601 ctgctgactt ctgggaaatt gttcggaatc atcggtgtga tcttggggat tcctgtttat
    21661 gcagcagcta aagtagtgat tactcatgtt tttgaatggt acacaacaat ctcttctctt
    21721 tatgaagaaa atccgttgaa aaagcagaaa gaataacaaa aagagacaga tgggaaaata
    21781 tccaaggcac aaatgctgct ttgaagattt tttcctctgt ctcttttgta tgcatttatc
    21841 tcaattttta aaatgattta cctaaagcag cagctttatc taatgcattt tgcatcaact
    21901 catctgcacg gtcagggtca taatcgattc cttcgatgaa tagctgatct acttgatcga
    21961 tgcctacaaa gtttaaaata ccttttacat attgagaggc aaagtcttgt ccgttgtata
    22021 caccgccatt agattggatg tgcagtgctt ttttacctgt cactaatcct tctggcccgt
    22081 tttctgtata gcggaacgtt tttccagcta cattgacggt atcgaaccaa gcttttaatt
    22141 ttgttgggat atttagattc cataaggcgt ttgcgataac gattttatca gcagcaagga
    22201 actgatcagt caattcattg aaacgagcaa ttttttgttg ttgactttct gataaggaag
    22261 tgaattctgc accagcacgt aatgcttccc aacctgataa taattcttca tcgatttccg
    22321 gaatatcatc gcgataaagt tccaaaacag tcacttcatc atctggatga ttttctttat
    22381 aacttgttaa aaaagacgtc aatgccttaa ctgtgcgcga ttcttctgca gttaggggat
    22441 gacctttgac tactaaaaca gatgccatat gcttaccttc tccttttatt gctatgattg
    22501 ttataactat gactaacaaa atcagtttac tcgaaaaaga aaaggctatc aagtcatatt
    22561 gataaggttg aagaataaat aactaaaaaa agacagggta taaaagaggc tgtgacacga
    22621 ttttttgtca cagcctcttt tgaatctaac aagcaatatt caagcaacac tcgactgaag
    22681 agcttccatc ccaccaatga tataaacagc acgaaaacca ttagcagtaa gttgatcggc
    22741 taatgcttct gatcgcctgc ctgtatagct tagaatataa taaagtttct ttggatctag
    22801 attttttaat tgtcttggca agatagcagc ggggatagaa agcgagtggc ttaagtggtt
    22861 ttttgtgtaa tctaaggtat ttcttgtatc taaaacgatg ctgtcagcat cgagaggcaa
    22921 tttattgaag tcttggacac taattgattg ggccatttat ctcatccttt gtttagtatg
    22981 tggctatttt aatgggcaac tctcattaat tcaaatggaa ggattcctct tatcactttt
    23041 ttaatgtatc ggatgataat gaaaaaaact atgcaaatgt cttgcaaaac aggtaggagg
    23101 tgttataatg acatagcgtc atatgacaat gtgtcatctt gaaagaaagg agaatccata
    23161 tgccgacaca gacatttttc catttgccta aagaaaaaca aaaaagacta atcgaggctg
    23221 cgagaatcga gttttctaga gtacccttaa aagaagcttc gattgctaat atcgtcaaat
    23281 tagcggatat ccctagaggc agtttctatc agtattttga agataaagag gatctttatt
    23341 tttattactt tgagacaatg agacgtgaca gctctaaaga tatgttacaa ctaatgaaag
    23401 aatccgatgg tgatctattc aaaggattcg aatcctattt ttctaaaatg atttatgaga
    23461 ttttggaagg cgagaatgct tctttttatc gtaacttgtt tatgaacatg gattaccgtg
    23521 cctcaagaaa agtcgctcct catacgagtc gccaaaaaac gcctcacgat ccacataaag
    23581 aacacaaaca agatgcaaaa gagttgcttg aattgatcga tcggacgaag ctgaaagtaa
    23641 aaaatgatca tgaattggaa ttactgatcc aattagtgat gaatactgta tttacaacaa
    23701 ttgcgcattc gtatcgctta ttttcaacat ctgaagatta ctcagtagag caagcaatca
    23761 gtgaattcaa tttgaaactt tcttggctga aaaacggagt atataaatag aaagaaggga
    23821 actagatgct aaaaatcgta aaacggatct ccttgacttc agcaattgca gctgcagttt
    23881 tcatggtgat ccaagtactg gcagatttat atttgccgac acttacctcc aatattattg
    23941 ataaaggggt agcgcaagga gatgtcgatt atatttggca tacaggtttt gtgatgatcg
    24001 gcttttcttt gatcagtatc ttagctgcta tcgcaaatac attttttgct actcgtgaat
    24061 cacagaaact gggtaagcaa ttgcgtacgg atgtctataa aaaatcagag agtctgacaa
    24121 aagatgcgtt tgataaatat ggaacggcct ctttgatcac aagaacgacc aatgatgtga
    24181 ctcaaatcca aatggttaca caaatgtttt tacgtatgat gatcaatgca ccaatcacgt
    24241 tgattggcgc gagcatactt gcttatcaaa aagatcatca gttgacaaaa atcttcttag
    24301 ttgtcatccc agtaatgatt attttgatcg gcggaatcat gtactttgct gttccattat
    24361 tcaaaagtat gcaaaagaaa actgaccgtt taaacctggt atttcgagaa ggattgacag
    24421 gggtcagagt gattcgagca ttcgataaga cacgctttga agaaaatcgt tttgatttag
    24481 cgaataaaga ttatacaaat acggcaatca aagttaatac gatcgtcgca ttgatgatgc
    24541 caatgatgac attgatcatg agcggtacaa acgtagcaat cacttggttt ggtggacact
    24601 acatagcaga tatgactttg gaagtcggta atctgattgc atttatgact tatgcgatgc
    24661 agatcttgat cagctttatg atgctttccg ctatttttat catggtgcct cgtgcacaag
    24721 cttcagctga ccgtattaat gaagtgttgg atgaaaaaat cggtatccat gaccctgaaa
    24781 atcctaaaac agtttctttt gctggaaaaa acgcaacatt ggccttcaat catgtcaatt
    24841 atcgatatca tggagcagaa aaacttgcat tggaagatat tgatttccaa gccaagtcgg
    24901 gcgagatcgt cgcaatcata ggggggactg gttcaggtaa aacgacctta gtcaatttga
    24961 tccctcgatt atatgacatc gaatctggtt cgatccaaat aaatggaacg gacattagtg
    25021 acatgactca atataatttg cgagaattaa tgggctttgt tcctcaaaaa gcagttctgt
    25081 tctctggaac gatccgagac aatatgcagt atggtaagcc ggatgcaaca gatgaaatga
    25141 tttggaaagc attagaaatc gcacaagcta aagattttgt ttcagagatg gaagatggct
    25201 tagatagcca tgtggaacaa ggaggaggaa acttctctgg tggacaaaga cagcgtctgg
    25261 caattgcacg ggcattagtc aaaacagcag atatctatgt gtttgatgat tccttctcag
    25321 cgttggattt caaaacggat gccaatcttc gccaagcatt gaaaacgaat atgaaagaaa
    25381 gtatcaccgt tttagttgct caacgagtaa gtacagtaat ggatgcagat atgatccttg
    25441 tgctagatga aggaaaactg gttggcaaag gaacacatga agaactatta gcaacaaacg
    25501 aaacttatca agaaatcgtt cattcacaac taagagagga ggacttagca tgagtaaaac
    25561 aaacacacgt ccctctcatg gaccagcagg cggtccagga catggtggac ctggcagaaa
    25621 tctcggtgta aaaggtgaaa agcctaaaaa tttctgggga acggtacgcc gattgtttgg
    25681 ctatatgtct aaacgtatgg ttgcgatcat cgctgtattt gtactagcaa tagcagccgt
    25741 gatcttccaa atccaaacac caaaggtctt ggggaaagca accacagaaa tattcaaagg
    25801 tgtaatggct ggcgctcaac agatgcaaca aggccaacaa gtaacaaaat ttccgataga
    25861 ctttgataaa atcgcgcaga tcattgcgac agtaatcgtg atgtatttga tttctgctgt
    25921 attcaacttt ttacagcaat ttattatgac acgtgtttcc caacgaacag tttatgaatt
    25981 acgtcatgat ttggaagcca aaatgaatcg tgtaccgatt tcctattatg atacacatac
    26041 aaatggggat atcatgtctc gagcaatcaa tgatatggat aacatcgcaa gcacgctgca
    26101 gcaaaatttg actcaatttg taactagtgc agtcacattg attggggtca tcgtgatgat
    26161 gctttcaatc agctggcaat tgacgttagt cgcgttattg atgatccctt taagcttgat
    26221 catcgtgatg atcgtagcgc cgaaatcaca agtgtttttc gcagatcagc aaaaaagttt
    26281 aggtttattg aataaccaag tagaagaaac atatggtggt cataccattg ttaaaacatt
    26341 caatcatact gaaaaagacc aggaagtatt tgaaaaagaa aatcagaatc tgtatgccgc
    26401 tggttggaaa gctcaattta tttcggcaat catcatgccg ttgatgaatt tcgttaaaaa
    26461 tctaggctat gtgttcgtcg ctgtactagg cggagtaaaa gttgcaaatg ggaacatgac
    26521 attaggggat gttcaagcgt tcttgcaata tacaacacag ttctctcaac caatcactca
    26581 acttgcaagt ttgatgaaca ctatccaatc aacagttgct tcagccgaac gggtatttga
    26641 agtgttggat gaagaagaga tgtccaatga aaaatctggc cttccagaag aaaaagatac
    26701 accatacaaa gttcgttttg aaaatgttca attcggctac acaccagata acttgctaat
    26761 gactgatttt aacttggatg tcaaacctgg tgagatggtc gctattgtag gtccgactgg
    26821 tgcagggaag acgacactga tcaaccttct tgaacggttc tacgatgttt ctggaggtag
    26881 tattcgttac gatggtgtag atacccgtga tctttcacgt gatgaattgc gcgcacagtt
    26941 ttcaatggta ctgcaagata cttggctgtt tacaggaagt atctatgaca atatcaaata
    27001 tgggaacgac gatgcaacag acgaacaaat cattgaggct gctaaagctg ctcacgtaga
    27061 cgattttgtt cgtaaattgc cagaaggata taacactgta ttaaacgagg atgcaagcaa
    27121 tatttcgcaa gggcagcgtc agctaatcac aattgcgcgg gcattcttag caaatccaga
    27181 tgtcctgatc cttgatgaag caacatctag tgtagataca cgaacagaaa tactgattca
    27241 aaaagcgatg aaccgcctat tggaaaaccg gacaagtttc gttgttgctc accgcttgtc
    27301 tacaatacgc gatgcagaca acattattgt gatgaatcat ggttcgatcg tagaaacagg
    27361 aaaccacgat acattgatgg aaaaagacgg attctatgca gatctttata acagtcagtt
    27421 ttctgaagaa gaagcttcat agaatagtac aaatttatta ttttttttag gggctgtgac
    27481 aaaagtcgtg tcacagtccc tttttccgaa taaacggtgt tcaatcagct gtccttcggt
    27541 attagctcaa aaaacggcaa aatatgagaa gctatttttc cgttttgtat ctctaatact
    27601 caggtcaaac cgctgatttt acaacctctt ccttaaacgg tgttctcctg ccaactcctc
    27661 ggtcacaagt cacaatagtc aacaaacctg agaagctgtt ttttgaaatt gctccttgtg
    27721 actcggagtt aaacggcagg ctcacaacct cttggtaaac ggtgttcaaa agccagtttc
    27781 tcggtattaa gccaaattta tgcaaaaatg tagaaagcca ttttcacaaa atttttctta
    27841 ctactcgaaa ctaagcgact ttttcacaac ctcttcctta aacggtgttc aaaagcttgc
    27901 atctgcggta ataagcccaa ccaaacgaaa aataggagga gctatttttg tttggctgtt
    27961 cttattactt gatgcagaac agctttttca caacctcttt ttataagagt ttttactcat
    28021 taatttacaa aaaacaagca aaatgcgaat tttctcacat tttttttaac aaaatcaaag
    28081 ttattgcttg aaagaagaag aatccatcgt taaaataaag cttgacgtag aggggagcaa
    28141 gaaatatgaa agattttcaa ttgggggctg ccacctattt aaaaagtgta gccattcggg
    28201 tgaaagaccg agataaaatg attgattttt acaagaacgt tgttggcttt gatttgaaaa
    28261 gagaagaaaa cgaattagcc attttaggaa cagaagaagc tggaagtgaa atgttgctgc
    28321 tggaagaaag tcctagagcg aatgatcaca tgggggtgat caaaaagctg aaccgttttt
    28381 cattgattat ccctagcaca gaagaaatgg cagatatcct ttgtcgtatc cgtaaaatga
    28441 attatccaat caaagaagct ttagaagatc atggacgtat ggggattttg ctcgtagacc
    28501 cagaagagaa tgaattggaa atttaccatg aagagaataa gaacgaccaa agagaaattc
    28561 cagaaacaat ggatcaagaa gcattattaa gaaaatctga agaaaaattc agtaaactat
    28621 caacaggttc ttattttgaa aaaatccatt taaatgttac taacttaaca aaagaacgcc
    28681 aatttcttaa tcatgtactt ggtttgaagg tacataatga agaagagggc atccatgtat
    28741 tgaatgaagg agatttccat gttggtttga cagaagctag cggaggcgca attgatttgc
    28801 ctacagatga agtgattgga cttgattttc tgaaattcat gatcgatact gaaagcatgg
    28861 aaggactgat tgctcacttg cagcaactat cagttgattt cttcttagac aaaaagcaca
    28921 cagtactcac aatctatgat gctataggaa tcgaatggtg gttcgtcagt gagccaaaag
    28981 aaaagaataa aactgacatt cgatgcgacg cttaactttt tctttcttaa cgattgtcgc
    29041 atgtctcgtc attttatgaa cggtcatact atcgcttatt ctagagatag aaagagaaaa
    29101 tagtataccg ggggaaactg cgcggttttt cgcgcagttt ttttgtgtaa aaaaggttga
    29161 ttcctcatag attaagcttt aagcttggac tgtaagaatc tgatagaaaa gcaggagaaa
    29221 aagatgtatc cacacacgaa acagcttatt ttgaaaaaga tggaagaagg attttatcca
    29281 ggtgtagcag tcgccttcat aaaagaaaat caagtagaaa catttacagc cggctttgcc
    29341 caagtcgttc ctgaaaagca gctgatgaca gaagatctgt tgtttgatgc agcctcactg
    29401 acgaaagtcg ttgcaaccac ttcattagtt ttacgtctat tagaagatgg aaagatagaa
    29461 ctcgatcaac cgcttcataa atatcttcct gaatttgaaa atcagacaat cactttgcgt
    29521 catttactga cacatacttc tgatatcaaa acatggatac caaacagaga taagctatct
    29581 caggaagaat tgatcgccgc ttatttgaag ctagaaccag gtgagcaact aggaaaagaa
    29641 gtaaaatata cagatgccgg tacgattctt cttgggttga tgctagagga aatttaccaa
    29701 gaaactgcag tggaaatctt tcaagaacaa gtattggaac cattaggaat gatgaacagc
    29761 ttatttcttc cttttccttc caaaaaaatc gttcccacag aacaactgcc ttcgggtgag
    29821 gttttgcgtg gacaaactca tgatccgaaa gcaagagttt tggctgaaca tgcaggtaac
    29881 gcgggtctct ttattgattt gaaagacagc attacttttg tccaaatgta tttgaacaag
    29941 ggaaaaatcc gtaatggtcg ttttttagaa gaagagacga ttgattcttt actgcaagat
    30001 caaacaccga aaaaaaatgg ctatcgttct ttaggatggg acttaaaaaa ggatgtagaa
    30061 gacggtcatg cgattctttt tcatactggt tatacaggta cgtttttctt gattgatata
    30121 aaaacacaag aagcgttgat atttttgtca aatcgcgtac atcctgtcga tcatcgtgcg
    30181 gagtatctca tatgtcgaga tgaaattgtt gaaaactttt tgaaagagaa agcggtatta
    30241 ccgaaataat gctataatag ctatgaaaaa tgagaggagt ttgaattatg caggaaccta
    30301 tgtttttaaa accagttttt caagaaaaaa tttggggagg aagtcgttta cgttcagtct
    30361 ttggttttga tatccctaac gataaaatcg gagaagattg ggcgatcagt gcgcatcctc
    30421 atggtgtcag tgtagtagaa aatggcgaat tcaaagggaa aagattagat gaactatggt
    30481 cagaacacaa ggaattgttt ggccatcctt cagaaccagt gtttccatta ttgatcaaga
    30541 tcttagacgc agaagatgat ctatctgtac aagtacatcc agatgatgcc tatgggatga
    30601 aacatgaagg tgaattagga aaaactgaat gctggtatat catcgatgct gaacctggtg
    30661 cagaaatcat ctatggtcat catgcaaaga caagagaaga actagctgag atgatcaaag
    30721 atgggcgctg ggatgacctt ttgaagaaag taccagttaa aaaaggtgat ttcttttatg
    30781 taccaagcgg aacgatccat gctattggta aaggaatcat gattttagaa acacagcaaa
    30841 gttctgacac gacttaccgc gtgtatgact acgacagaaa agatgcgcaa ggacaaacaa
    30901 gagagctgca tatccaacaa tcgatcgatg taacaactgt ccctgcgaaa acaccagaat
    30961 tacaaatcaa agaagtccga aaagggaatt catcgatcgt cacttattta gaaactgagt
    31021 ttttcaatgt atatgagtgg gatattaaag gaatcacatc tttcaaaaaa caagcccctt
    31081 atacattgat gacggtaatc gaaggagcag gagaccttgt cgttgacgga aaaacatatc
    31141 cattagaaaa aggaacaagc tgtattattc caaatggcgt ttctgagtgg gcggtccaag
    31201 gtgaactagc aatcattgcg tctgaaccag gcaaatagtt cccagatagc tgaaagcagc
    31261 agacggtttt tcgtttgctg ctttttctac ataaaatccg tccatcaata aatagaagtc
    31321 tggtgaaaaa gatggaatac aaatggaaag caaacaacag cagtcgcgtg tctatgaaac
    31381 aactgagaaa agctggacat cacttaaatg aaatgatcat gccaaatcta agtctgttta
    31441 tcgcttgggg aatcttgtca ttagcaacaa aaggtgtgtc tggcgatttg cacaccacat
    31501 tagaagaggt atgccattgg atgatccagc tgcttttacc catcttgatc agttattcag
    31561 gcgggaaatc ggtaggggga caaaaagggg cagtcgccgg tgcaattgct tctttagggt
    31621 tgatcgctcg ttccaatgct ccacaaatcg taggagcgat gattatcggg ccgcttgccg
    31681 gcatcgtata tgaacaattt gtttctagat tccatgtgaa attcaaggct ggatatgaaa
    31741 tgcttgtcag caacctacta gtaggactga ctggtagcgt tatttgtata attggaatcg
    31801 tttttattga accagtttta aatagtgttc acttgttgct cgcctccgtt gtgtcttggc
    31861 tagtcacaat gaatctgtta ccacttgtca gtctgatctt agaacctcta aaagtactat
    31921 ttttcaataa tgcaatcaat catggtgtat tgacaccttt aggaatcgaa ttctcacaaa
    31981 cagtcgggta ctcttcctta tttttgatgg aggcaaatcc tggacctggt ttagggatct
    32041 tactttctat cctttgtttt gcagaaaaac aggaaaaagt aaatgcgagt ggagcattga
    32101 tgatccatgc gattggcggg attcacgaaa tctattttcc atttgtcttg ctgcgaccgt
    32161 atctattttt agctgtaatg gctggtgggg caagcggcac gttgatattt caatggttag
    32221 atacgggatt agctgcacct gtatcgcctg ggtcactact cgtgattttt gccaatgcgc
    32281 cttatccaca actagctggg gtaagtatag ggatagcaat cagtacattt gtgaccttca
    32341 tttgtgcagc gatactattg aaattaccaa ataaagaaaa agagaacaaa acggtcaaag
    32401 aaaggggaga aatcttggaa ccatctatca ttcattccgt catttttgct tgcgactcag
    32461 gtatgggatc aagtgctatg ggagccgccc tgctgaaaaa gaaattagca gcagaaaaaa
    32521 tcgatctttc tgtcggatac agctctatct atgaattgaa taaccaagta aatcagctgg
    32581 ttatcgtgca acatgaacta gcgggtcttg caagagaaaa agtacctcaa agtcaaatct
    32641 atgtactgga tcactttttg gaaacagata tcttgctgtc acgaataaaa gaagcgatcc
    32701 aatttccaaa gcgggaagaa aaaaaagaga tagcagatat cacagaacaa aaaacgtcgc
    32761 ctgctaatat tgtatttttg tataaaaaca acagacgcgg ctcacaaact atgggcatga
    32821 cgattttaag tcaattagca aaaaaagcga aaaaacagtt tgttatctca aaagaatcaa
    32881 tagaaaaaat caaaatccaa gaagaaacga tttatatcgc acaaagagaa ctagctgtaa
    32941 ctgaacagct agaagaaaag atccctcggc tagttctagt tgaacattgg gtagctacga
    33001 atgaatacga aaaactcgtg aagggagaaa tataagatgt atttatcgcc cagagaaaaa
    33061 caattattag cagaatttct cagtagcccg tcacctgtat ctatccaaaa aatgatgaat
    33121 ttattgaagg tcagtaaacg gactatttac cgcgaattag ataatcttga agtctcattg
    33181 aaatcggtaa atgcttcttt gaaaaaagca gctagaggct catttgtcat tgaggcatca
    33241 gaagaagtat tagagcaatt aaaagaaaat acagatggga aaagtaatga cttatccact
    33301 gctaaacgac aacacgcgat tttagccgag ttgttactga cgacagagcc gttgagtctt
    33361 gctcatttca tggaatcata tcgcatcagt aataccactt tttatgcaga tatcaaacag
    33421 ttggaagaaa gtattcgcca actgcctttg gaaatcattc ggaaccaggg atacgagatc
    33481 gaaggaccgg aaaaataccg ccggcttctg acagcgaatg tattagaatt agaaatcaat
    33541 gaatatgaac tttttcatag tatttctttc gatgcatcac tgaattattt ttttcgattc
    33601 gttgatccgc aacatctatc tcttgcacgc aaagtaatcg gggaagaatt gatccaaaaa
    33661 aagaccaatc taagtgatag aaagcttgaa catttggttt tgatgctgac attgacgatg
    33721 gagcgtgtga cgaaaaatca tttactgaca aatgagacat ataatagtct ggcaaacaaa
    33781 gaattactac agacagctaa acgattgttt tcacggatcg cttctgagac aaaacaactt
    33841 tatccagtaa atgagatcgt cttttttgca ggattattaa atgacttcac gaactcattt
    33901 gatcagaatt tctttgaaga gacgttcgat acacagcttg cttatttagt aaaacaactg
    33961 attcaagaag taagtgaaga gacgaatgtc catttttatg aagatgaaac tttgtacaaa
    34021 atgctgttga ctcatttatc tggtgtgttt tcaagagccg ttttacaaga agagacactg
    34081 accaatccga ttttagaaaa aatcatggtt caatacgagg aagttgcgaa tgcaatccgt
    34141 aaagcagtag agcggatttt tcaagaaaaa aaattatccg aagaagagat cgcttatatg
    34201 gtccttcatt ttgcgaattc attggaacga agtccgaaag aaagcaatat cagtattgca
    34261 ggattttcgc caagtggatt agcttctact agcatgttgg aactacgatt gaaaaggtat
    34321 tttcccttca tccaacaaat tcatttctat cggattgctg atttaaaaaa aatcgattta
    34381 caagaagaat atgatgttgt tatttctaca tctattttac caggatacgg tggaaaatat
    34441 attttagtct ccccgctact gctagaagat gaagtcaagc agctaaaaga agctttttcg
    34501 catattgaga agaaaaaaag gcaaaaaatt acgtataaca gcaataacga gtggatgata
    34561 gaagatactt atgaggaaac aatgcatttc atggatcaaa tcaatcaatt actcggtcgt
    34621 ttttttatcc aagaactgga taatcctgag acgatttccg aaacgattcg cagcttattt
    34681 ccttattttc ctgaaggact agtaacggat gaagaggtgg ttcatcgccg gctgcttaag
    34741 cgatatgaac aagcacctat cggtattcct catacaaata tggggttatt ccatacatcc
    34801 agctcattga tttctaaacc gatcttttgt attttcaatt tgaaacatcc gttgaccatt
    34861 gaagggatgg atagacagcc agtcgatttg tcccgtatgt tagttatgct ggcgccttcg
    34921 ccaatccaag aaaaagacgg gattctttta ggaaagatca gtggagcaat catcatgaat
    34981 gacttgaaca ccgagatctt ccgctcagga aataaagaaa tcgtttatca gttgttagcg
    35041 aaaatcctga ttgaagaaat aaagaaatga aggtgaaaag atgatacagc taccagcaga
    35101 aaaaatattg ctcgatcaat cctatcaaaa ttggaaagaa gcgttgaccg agatcggcca
    35161 gtggatgacc caacaagggg gaatcaaacc ggcatatatc caatcaatga tcgaaagaca
    35221 agaaaaagcg agtgtctata tcgggaattt cgtagccttg ccacatggga ctgacaaaga
    35281 tgaagaagta gtagaagaag gaatctattt agtccaagtg cctgatggtg tggactttgg
    35341 aacaaaagag gaaccaaaaa tcgcgacact cttatttgta gtggctttaa aaaaagaaca
    35401 tcagttagcg gtgctccaag atttagcctt tttctgttca gacatcgacc aagtaatggc
    35461 gctcagtgat gcggaaacca ttgaagaagt ccaaatgatt tttgaacatg gacatatata
    35521 accaatgttc acagtaaaaa aagctgcctt gcatcaagta ataacttcaa ccaagataaa
    35581 aataagagga gctatttttg ttgggaggtt cttattactg cggatgcaag cttttgaaaa
    35641 ctatttattc tagaaaaccg tttatttgaa ataggggacg gtgacaaggt ttttgtcacc
    35701 gcccctatta tttttgtgtt ttaatagctc ttttttatat agatagtgcc catacctatg
    35761 aataataaaa cgatactcca tataccaagt gtcaagatac cattcatgaa agtaacctgt
    35821 tctgaagcta gtggcgagaa aatatctcct cctatgatca cattttgaaa atcaacataa
    35881 gaagttggca agtaatgact gattgattct atccatccaa tcgaagaaaa caagcgatac
    35941 tctgctaaga aaagacaaag aagtgtacct aaaagattga ctagagaatg ttcaaataaa
    36001 gcagatagaa gaaaactgat tgtacttaaa aagagcaccc acaacaacag gaacagaaag
    36061 ctctggaaaa gatactcata catcggcagg attcgtatct cctgaccaat gatcgttcct
    36121 acaggataac ggaagtctcc caaaccattt ttgattatta ctagtataga gacgatcaaa
    36181 aatggcatca cgagacttag catagctgca ccataataaa tggtaaaacg gatgctatgt
    36241 ttctttacca tactatccgg taatagattg actagagaga ttacctgctt tcttttttca
    36301 taagagatga tggaagaaag aaacaaggcg gttatacata aaatcaaaaa actagaaatc
    36361 attcctgaaa aaatattttc gtagtaattt gctaaagaga gatgcgctgg caagtcaaaa
    36421 atagaatatc tttggatctg atgttctttc atataacgga gcaactcgac acgtttttgt
    36481 tgttcaatga ttggaattcc ttgtctttga ccagaaatca gtcgatccag tatatctttt
    36541 tcataatgat aagttgcttc gaccatttgc tggacattcc ctgtgttttt tgcatgtaag
    36601 attgcttcga tcagtttgtt gcttttctct aaactttcca caaattcttt ctcattctca
    36661 tcttctttca acgtttcaat caaacgttgc atctcttcat agttatgatt cagttcatcg
    36721 aattctgtat aagcaagttt atgatcaaaa aaaagtggga atgcaaagag cacgaccaac
    36781 aatagaggaa ataaaaagtt tactttgttt tttagtaaga gattgcgttc aaatgtaagt
    36841 aaagacatag ttacacctca tataatttac gataaatagc taaaatctct tccatatttg
    36901 aggattctgt gacgatttct ccatttttta gaaaacatgc tttagaacaa atcgattgga
    36961 tattcgcgat agcgtgggaa gaaagaagaa tcgtcttccc ctctttctgc cattgtctga
    37021 tgaggctatt caagatatca atacttcctg ggtctaatcc gttcaacggt tcatcgaata
    37081 acatgacaga tgaatcactg atcaagtaag cagcaagcaa gacatgttgt ttcattccta
    37141 gagacatttt tccaatcggc aaagtaaggt actcagtcat cttcaatcgt tggaaaacag
    37201 tttggcattc tactttgctc tgccaatagt atttgatcaa agtcaaataa tcaacagccg
    37261 atagatagtc ggataactga ttcgttgatt ctagaaaaaa gaaagaccgg cgataatctg
    37321 tataagcagt aggagagatg ttgtgaaggg acaattgatc ataagttgtt ggcagcagtt
    37381 gggcaatgcc attcagcaaa gttgttttgc caaatccgtt cggggcaatc aaagcagcta
    37441 cttctcctga ttctactaaa agattcaatt gtcgaatcac acgtttttta ccacgtttca
    37501 tgttcatgtt tttgatcagt aatgtcatag ataggcctcc aatataaaat ttaataacat
    37561 aatagaatgg aaaacatcta aattaaatgc taacaaaagt tattgtttgc atattataaa
    37621 aaaattaaaa taacagtttt atttaaaatt gaagttttct gataccgaaa tctttttatt
    37681 ttgctgacat actcaaacct ttggcacaaa gtttcgtgcc aaagtacgac tatttctctt
    37741 tttagtaagc ggatacaatt agagtatcaa aaggagagag ggagagacaa aatggaacgg
    37801 acgattccca aaaacaacat ttcgctaaaa gcgagggtac aaaaattagg cagtacttta
    37861 tcaagcatgg tcatgccaaa tattggagca ctgattgcct ggggtgtatt aacggctcta
    37921 tttatcccag atggttattt accaaatgag gctttcgcta ccatggtaag cccaatgcta
    37981 acttatctga ttcctttatt gatcggttat actggcggaa aagtaattgc tggtgaccgc
    38041 ggatcagttg ttggggcaat tgcaacgatg ggtgtgattg tcggaacaga tattccaatg
    38101 atgctaggcg cgatgatcat gggacctctt ggtggatatg ccatcaagaa atttgatcag
    38161 ctattccaaa aacggatcaa atccggtttt gaaatgctag tcaataattt ttcagcaggt
    38221 cttatcggtt ttggattagc gttattaggt ttttctgcga ttggtccagt tgtcgatgca
    38281 ttgactcaag caatggcaaa aggtgtagag atcattttat ctgcccattt gattccattg
    38341 acaagtattt ttattgaacc agcaaaaatc ttatttttga ataatgcaat caatcatggg
    38401 attttgacac cattgggaac tgaacaagta gcagaggctg gaaaatctat tctattcttg
    38461 cttgaagcaa atcctggtcc aggacttggc gtattacttg cttttacttt gtttggcaaa
    38521 ggagcagcaa aatcttcagc accaggagcc atgatcattc actttttagg cgggatccat
    38581 gaaatctatt ttccttacgt tatgatgaaa ccgttattat tcttatcagt tataggcgga
    38641 ggaatgtcag gaagtttcat tttccaattg atggatgctg gactaagagc gccagcttct
    38701 ccagggtcga tcattgcgat tctagcgatg acaccgttca attcttacct tcctgttatt
    38761 ttgggcgtga ttgttgccgc agctgtttcg tttggtattt ctgcagtaat tttgaaagcg
    38821 gatgcgaaag atacaaatga agacttcgaa aaatctgttc aagagacaaa ggcagctaag
    38881 caagcttcca aaggtgccca aggtattcct caaggagcta cattctctgg cgtacaaaaa
    38941 atcatttttg cttgtgatgc aggaatggga tcaagtgcga tgggcgcttc gatcttgaga
    39001 aaaaaagtac aggaagcaga cttgcctcaa acggtgacca atcaagcaat cagccaactg
    39061 acagatgact cgaatacatt gatcgttact caggtcgaac tacaagaaag agcaaaacaa
    39121 aaagcaccga gcgcgcgttt tgtctcagta gaaaactttt tgaattcacc tcgatatgat
    39181 gaaatcattc aagaattaag cagcaagcag aaaacatcag tttctgaaat tgagaatgag
    39241 ccagcagaag agtttctttc agcaaacatc aaacaaatca aagagattct acttgtccac
    39301 gatgatcgtt ccggatcagc aacaatgggg atgaccgtgc tcaaagatat attagacaag
    39361 aaccatttaa ctatcccgtt aagaaaagtc aatatccaag atttaaaaga tgaacctgat
    39421 tcactgatcg tagccaaaga aagtttagcg gaaaaagtac agatggctgc acctcaatcc
    39481 attcatttat cagtcaacag catggtcaat ccgcaaaaat acgaatcgat tgcacaatat
    39541 ttgaaacaga cagcttaagg gaggaaaaca gcatgcaaag tttgccattc gacatgatcg
    39601 agctcaatcg atcttttaaa accaaagaag aagcgatccg ctattgcggt cagatgcttg
    39661 tagaagcagg gtgcgttgat gaggaatata tcgatgccat ggtagataga gacaaaatgt
    39721 tatctgttta tatggggaat tttatcgcga ttcctcatgg aacagatgaa gcaaaaaaat
    39781 atgtgaaaaa atccggcatc tgtgtcgttc aagtaccaga tggtgtagat tttggtaccg
    39841 aagaagaaga aaagattgcg actgttttgt tcggtattgc aggagtcgga gatgagcatt
    39901 tgcaacttgt acagcaaatc gcactttatt gcagtgatat ggacaatgtt gtccagctag
    39961 cagacgcgct cagcaaagaa gaagtaacgg aaaatctagc aattgcttaa ggagagagaa
    40021 aaatgagagc agtacatttt ggtgcaggaa atatcggtcg aggatttatt ggagaggttt
    40081 tggcagctaa tggatttgag attactttcg tagatgtgaa tcacacgatc attgatgctt
    40141 taaatgaaag aaaagaatac gaaatcgaat tagcagatga atcaaaagaa aaaatcactg
    40201 taaaaaatgt caaaggaatc aacaatcaaa caaatccaga aaaagtaatc caagaaatcg
    40261 ctgaagcaga tttagttact accgccattg gcccgaatat cttacctttt attgctgagt
    40321 tgattgcaaa aggtatccaa gaaagagaaa aggaaggaag cacaacatct ttagatatca
    40381 ttgcctgtga aaatatgatt ggcggttcag catttctaga aaaagaagtg tataaatatc
    40441 ttccggaaac atcatttact gacaaatata tcggctttcc aaatgcagca gtcgatcgaa
    40501 tcgtcccatt acaacaccat gaagatcctt tattcgtcca agtggaacct ttcaaagaat
    40561 gggtcattga cgatagccaa agaaaaaata aaaaaatcca attaaaaggg gttttatatg
    40621 tagatgatct agaaccatat atcgaacgca aactatttag tgtcaatact ggtcatgcga
    40681 ctgtcgctta tacaggtgcc ttgttaggtt accagaccat tgatgaagca atgcaagatg
    40741 cgttagttgt ggcacaatta aaatcagttt tgcaggaaac aggaagtttg ctgattgcca
    40801 aatgggggtt tgatgcagag cagcatcatg cgtatattga aaaaatcatt catcgcttcc
    40861 aaaacaaaaa tatctctgat gcgatcacac gtgtcgctcg gacaccatta cgtaagttag
    40921 gttatcaaga aagatttaca cgtccagtga gagaactaca agaacataac ttgacttgcc
    40981 cacatttgac ggcaacaatg gggattattt ttaattacta tgatcctgaa gatgaacaaa
    41041 gccgtcaatt gcatgaaatg aagatccatg aaaacttgga acaattgatt caagaagtca
    41101 caggaatcaa tgatccgaaa acaatcggga atatcaagca aaacgtgaac cgttatgcca
    41161 agcaagtcgc ttaaaatagg atacgaaaaa ggggaatgtg acgaaatttt tgtcacattc
    41221 ccctttttcg aataactagg ctgtttgtcc ttcaagatag ggcagatcca attctttacc
    41281 gtaacgttgt tctaaagcta ggcggatcaa acgagctgcc aggttctcat ttcttgcaag
    41341 gataggtcca tggaaataag aaccgaagac atttcggtaa atgacacctt cagattggtc
    41401 ttcaccatta tttccttgac cttgtaccac tttccccaaa ggtcgttcac cttcacctaa
    41461 gaaagtccga ccattgtggt tttcaaatcc ataataagtt tcattgaatt cttcgttatg
    41521 gataacgata tcaccgataa accggttatt ttcctgactt aacgtgtagt gatccagtgc
    41581 actgattcct tggatctttt ctccattagc tcccatgtag tagtgaccta gcaattggta
    41641 gccaccacaa atggctaaca tgacgccatc attttcgata tagttgatca aagaatcttt
    41701 ctttttctgg atgtcttttg aaatgattaa ttgctcaaag tcttgaccac cgccaaagaa
    41761 aaccaagtca tatttatcag catcaaaagg ttcatgtata ctaacgatct ccgtatggca
    41821 agaaaccccc attttttctg ctatatattt caacatcaaa aggtttccgt tatcaccata
    41881 tgtgttcatc aaatttccat aaaggtgggc gatattgagt tcataattag ccacggttca
    41941 ttcctccttt gataaatcct tgagcagcta gttctttacg aagctgcaat acagcagtgt
    42001 aagtagctaa aatatagaca tgctcagttg gcaattcttt gattttttcg attacttctg
    42061 gtaattcttt tgtttctgtc aatttatttt ctggaatccc ggcaactttt agacgtaaag
    42121 tcatatctgt atgacggtct cctccagaaa taacagctgg gatatccata tcggcaaagt
    42181 tttcaaaatt accatcccaa atccagctga catcgattcc gtctgcgtaa ttggcattta
    42241 ataaagccac taaagaaaat ggataaggag ctagaccaat catatcgatt acttgattga
    42301 tacctacagg atttttgaca agtactaatg tacatttttt cccattgata ttgatgactt
    42361 cttgtcggcc gaatactttc tcgtcatatc ctaaaccttg tttgatttta tcaggatcga
    42421 cattgaagta ctctgccact gcagtagctg ctaaagcatt gtagacatta tacataccgc
    42481 caacttcgat tccgtattca tgaccatcga tcacgaaatc tgctgacaca ttgtccatcc
    42541 gtaccatatc agttaatcga taatccaatt caggacgttt gaagtcacaa tttggacagt
    42601 aatatttccc taagtttgca taagtgatca ttttataatg caagatgtga tgacaatttg
    42661 gacataagac accgtctgta ttgtaatgag ccatttgttc acgatcttct tcatggtcaa
    42721 agccataata ttttctagga ttgactgttt cgatagaatt gaagattggt gaatcaccgt
    42781 tactgataat cgttgcattt ggtgactttg cggcaccgtc tacaatcatt ttataggtcg
    42841 tatagatttc tccataccga tccatttgat cacggaaaat atttgtaaat aagaacagtt
    42901 ctggtttcat atattctgtg actttgctca aacttgcttc atctatttct aggacagcaa
    42961 actttttctg tccttttttt ggttttgcct gcaagaaagt cgatacaatt ccctgtacca
    43021 tattcgcacc agtagggttg gttagaactt catcgaattg ctggcgcaaa atattgactg
    43081 ttaaagcggt agtcaatgtt ttaccgtttg ttccggtgac aacgacgact tggtagtctt
    43141 ttgccaaact atctagtata tgtggatcga ctttcagcgc tagtttacct ggtaaacttg
    43201 atccgccttt aaaaaaagtt tttaatgccc attgcgacgt ttttccaact gcaatcgcaa
    43261 aatgactgcg tattcccatt atgtaacctc caaaaacgat ttgctgacga ataaattcaa
    43321 ctaatcatac catatgttga aaaaaaggtt aaattgcttt tcttattctt aagaaaaggt
    43381 tacggctgaa agtgcatgaa atcggacaaa atcgcatgat ttaacaaaaa aacagcttga
    43441 aacaaaatga ttgtttcaag ctgctgtgat tttattattc cctcttacct atattcccat
    43501 tttaaccgat aataattttc tcgcttggat attcatagcc tagatctcgt gtttcttttg
    43561 gcacgaataa catcaatgta tatagcattc cgatccgccc aataaacatc agtgtagcaa
    43621 tcgtgatttt gcccacaaca gacaagtctc cggtaatccc taaagataaa ccagttgttc
    43681 caaacgcaga agttacttcg atgatgatcg agatcagcgt ttgttcttct gtagcagata
    43741 aaaaaacgat acaaaagaaa cacataccaa gagaaagcat aaagacaacg actgattttc
    43801 gcacatcatc ttgatcgatt ctccgtccaa aaatattgat gttatcttca cttttgagga
    43861 aggaatatag atacaaaccg atgattgcta cagtagttgt tcgaatcccg ccgcctacag
    43921 aactaggact gcatccgata aacatcagta aagaaaaaat gatcagtgtt gtgatttgaa
    43981 aatcacccaa atcgtgaatc tgcagaccag cattacgagt cgtaatcgaa taaaacatcg
    44041 aattgatcca tttgacactc atattggaat cttggaataa atggtccttt tccaatagat
    44101 aaatcaagac agttcccgaa acaaacaaga taacaaaagc taacacagct aatttggtaa
    44161 acaaggaaaa acgaaacgga agcttcgcat tggaacgttt gtataatagc cattcccggc
    44221 actccatcaa tacaggaaaa ccaattcctc cgacaaaaat caaaaacatg atcaggatca
    44281 agaaaaaata gtcatgtgca aagggcttga tggaatcccc agtgacgtca aacccagagt
    44341 tcgtgacagc cgatatcgcc tgataaaaac cgtaaaaaac ggcatcacgc caacgatcaa
    44401 aatagcctct gtaataaaaa taaatagaaa agaatgtccc aaaaagtaat tgaaaccaga
    44461 ttaaaatagc aaaagtgatc cgaatcagtc ggacgatacc agacaaacgc ggttgattca
    44521 tatccgtcat gatcagctgt cgctgtttca gtgtgatccg tcgtttagaa aaaatgacaa
    44581 aagcagtaga aatcatcatg atccccaaac caccaacttg aaataatatt tccagcagta
    44641 taataccgtt atcattgaac acagaattga tatcaaaagt cgatagtcca gtcacactca
    44701 ctgtactgat tgccatgaac agcatgtcca caaaagaaac atggctatct ggttcgcgaa
    44761 aaatcggcat ataaaataga acgagtgata agaccgtcat caatatataa taaaaaacga
    44821 tgatttggat cgatgagaaa tgattggcag caaacttctg tcctcgccgc tgcagccttc
    44881 gaaagaataa atcaacatgc acagtactat cctcctgaca atagtcgttt ctaccagtat
    44941 acaaggggaa ttaggaatac tcaaagaaat tatcaaacag ataacaatac agctttcgat
    45001 taaaatagaa atggcttata ataaggcaaa tcctagaaaa aagaaggagg aggaataaca
    45061 aattgattaa tgctcaacaa atcgtagaaa aaatgaaaaa tcaaaaaata aattatgata
    45121 cagttctgaa aaaaatgatc atgcaatggg agaaagaaga agtaaagcct aagatattaa
    45181 tacatacatg ctgtgctcct tgcagtacgc acacattgga attcatgtct caatatgcag
    45241 agatcacgtt atttttttca aattcgaata ttcatcctaa aagtgagtat ttgcgaagat
    45301 tacatgaaca aaaacgattt gtagaccaat ttaatgagaa aactgaccat catgttgcgc
    45361 taatcgttga tgaataccgt ccaaatgaat tcaccaaaat ggttttaaca aaccatcttg
    45421 aaaacgaaaa agaaggtggt gctcgctgca gtgcatgctt caatatgcga ttagatcttg
    45481 ttgcgcaaaa agcgcaagaa ctaggatacg attactttgg aagtgccctg accatctcgc
    45541 caaagaaaaa tgcagctttg atcaatcaga tcggaatgga tatccaaaag atttacggga
    45601 cacaatactt gccaagtgat ttcaagaaaa acaaaggcta tgaacgctct ttgcaaatgt
    45661 gcaaagagta tgatatttat cgccaatgtt attgtggttg cgtgttcgct gccaagcagc
    45721 aaggatgcga tctgaaagaa atcaatcggg aagcaaaagc ttacttaaaa acatcaaccg
    45781 tagtttttga ggaaaattaa agaaatcttt cggaaaaacg taagaaaaaa ataaagaatt
    45841 tatggtagaa ataaggtaga ctgtataatt tgtccgtagt aaagtaagga aacaagaata
    45901 aatttcaatg aaggaagaga taagatgcga aaagtagcac ctattttgaa gccaatcgct
    45961 gtattaacaa tctcgacgat tgtcgcgact actacaggta ttgtgaatag ttccagctac
    46021 cgtgcaccaa gttcaagttc tagtactgta gaagaaacaa caccaactac ttcagtctcg
    46081 acatctgaac caacaacaac ggaaagtttg aatgaaacca ctacgacaag tacatgggac
    46141 gatacaacaa ctagttcgtc gtatgatgag acatatacaa ctgacagctc aagcgaaaca
    46201 agtaatacgg taccagatgg cactccatcg gattcaacca atccaagtac aagtccggat
    46261 acaagtgcta gctccgaaac gataccaagc agtacgactg tagatgataa cacgcaaaat
    46321 gatgatgcag cacgtacagc acagcaaaac caagattcag gaaataccca aacgtataac
    46381 cagcaagcag gtgattctca atgacatttt tactagacct tatcaacggg gtgcataaat
    46441 tccttggcta tttagacatc agtcctaaat atcttaaccg tggatatacg attttaagtg
    46501 tgattcccac gctttattta ttgagaattg tttatggact ttggcaaaac caaaactatc
    46561 ttcaattttt cttatatgga cttgcatttc tagtgcttgt ttattttact gttttaaatg
    46621 tattttatta ctttttagat aaaaacacga aagccgatgt cactcagctt tttgtgaagt
    46681 atttacctga tgaagcgttc aacatccaga cagaagaatc gatagataat ggaaattcga
    46741 tcgataaagt gaatacacaa gaggtcatgg tttcttatga cgaagattat caattgaaat
    46801 tagccgagaa tatgcggtat ttgattggaa atggtgagat caaaacaaat gaactcggtc
    46861 gcatggatgg atttctggtc gatagaaata cattgtatcc ttattactat gtcaagaaaa
    46921 caggagagaa agagtatgtt ctaaatattg gacgaagcta ctcggacatg gaaaaagtag
    46981 ggaccattcg acttgactca acagaagaca cattggaacc tgtcggattg ttcatcgtcg
    47041 gcggtgattt tgtcaaagac gggttacgct atcatgagcc ttatcgtctg aaattgattg
    47101 caaaaaaggc acagtcgcag gaagaaccag tggtttatag tcgtagccaa agaagaagac
    47161 attcataatg tgatgaaacg ccgtgtatca gtggttgcga taaaaacgtt tattcggaat
    47221 aagggggatg tgacacgtct tttgtcacgt tcctttttta tataacgaga aagataagac
    47281 gaacacatca cttattcttt cctttcattt ataaacattg tagaataaag aaagtacaac
    47341 gaagtattgt gaaaaaacgt aataaataac gaggaaaata caagtaggag gtaagctgtg
    47401 aaagaatttt tgaaaaattg ggggattttg atccttgtat tagcggctat tttattagct
    47461 agagtgtacg tgtttacacc agttactgta aacgggcatt cgatggaccc tacattaagt
    47521 gatggacaac gattgatttc atctaagatc tcaaactatg aacgaatgga tattattact
    47581 acaaaggaac cgggggatga agaacggatg attgttaaac ggatcatcgg tatgcctgga
    47641 gatacagtca agatggaaaa tgaccagttg accattaatg gcaaaaagta tgatgaacct
    47701 tatctggatg agttcaaaaa agaattttca gaagataagt tacaaggaga atatgcttat
    47761 agctcaggat tccaagcaca agcagaaagt tcatccacct ttacgagtga ctttgaatat
    47821 actgtgccaa aaggaaagta tcttgttcta ggcgataacc gcttgatttc aaaagacagc
    47881 cgaatgtttg ggttagtcga caaagatatg atccaaggaa aagttgtttt ccgttattgg
    47941 cctctttcag aaattaaaat catgtgatga aaaaataaaa agaagctgtg acatgttttt
    48001 tgtcacagct tctttttccg aaaattcctt cttatttctt ggagttgctc actttttttg
    48061 caatctcatt ttaagctttt tggctgatgg ataagaaaag cgaagcgttt ttgttcataa
    48121 atagcttttc cccacgatgg aaggtatgac tagtagcaga gacaaagccc gttttttcca
    48181 atcgattatt caattcttct tggctaaagc cattatggac tttcggatga ctaatttgtt
    48241 cattcttgtc aaagtctacg atgatcaatt gaccgccagg agctaaaatt tcataaagtt
    48301 ttgtaagaat attctccgta tcaggaatgt gtaaaagtac aagtgataga aggatgagat
    48361 tagcttggac agcaggaaat tcgacagaag catcagcgtg gatcgttcgg acatttttca
    48421 gatctgctgc ttggatcttt tcttctgcca ttttcagcat agtttctgaa gcatcggcaa
    48481 tgatcagttc tttgaatctt tccgcaagcg gtaaactaac aagcccagtt ccgcctccgt
    48541 aatccagcag gactttttgg tggctgtcgc gcggtagata agtagttagt tcttcgcgaa
    48601 taatcttggc taatgcttga cggtcagggg aatcataatg gttagcgatg gaatcaaaaa
    48661 tattttttgt catcaaatag ctccttacgt aaaactttga aattttaact tattatagca
    48721 cgtgaaaaga aagaaagcat gctatactca attcaaaaag ggagaatcta gaaagaatat
    48781 gccgatatcg tttgtaaaag atagagagga aaaaggaaag tgtgtccgag aaatattact
    48841 agacttgcct gagtggtttg ggcttcctga atcaacggaa aaatatatcg aggaatcaag
    48901 taaactacct ttatggtgtg aaaaaagaaa agaagaatat ttaggcttta tcactctttc
    48961 acagacaagt gaagatactg ccgaaatcta ttctattgta tgggaataaa aaaagactat
    49021 catcaccaag gattaggaac aaaacttttt aaagaagcag aaggttacgc agcaaaacat
    49081 tacaaatatt tgcaagtgaa gactgtagac gaggggcact actctatata tgatcaaact
    49141 atttgtttct acgaatcatt aggattttca aggttggaag tttttccaaa tttatgggat
    49201 gaatggaacc catgtttagt tcttgtcaaa aaattggaac aaaagtgagc aatagattta
    49261 atactaatct gtgcttgatg aaaattctca ctgtaaaaaa tgctgaaaat caagtataat
    49321 accataggaa caaactggaa ggtgaaaaag agcatggcac aactattttt taagtatggc
    49381 gcaatgaata gcggcaagac gatcgagatc ctaaaagtcg cacataatta tgaagaacaa
    49441 aataagccag ttgtcttaat gacaagcggt attgatacaa gagatggttt aggcgttgtt
    49501 tcaagccgaa tcggactgaa acgtaatgcg atgccgatat ttgaagaaac agacgtattt
    49561 gacgtaattc agaaaatgga aagcaaaccc tattgtgtgc tgatcgatga agcgcaattc
    49621 ttaaaaaaga aacatgtcat tgcttttacg agaattgtgg acgaattgga tattccggtt
    49681 atggcatttg gtttgaaaaa tgattttcga aacgagttat ttgaaggatc aaaatattta
    49741 ttgctttatg cagacaaaat cgaagaaatg aaaacaatct gttggttctg ccacaaaaaa
    49801 gcaatcatga atttacatta tattgatgga aaccctgttt atgaaggcga tcaagtgcaa
    49861 attggcggaa atgaagctta ttaccccgtt tgtcgaaaac attactttca cccatcaatg
    49921 attacagaag gagaataagc atgtacgatc aattacaagc aattgaagac cgttatgaag
    49981 agcttggaga acttttaagt gacccagaag tgatttctga tacaaagcgt tttatgcagc
    50041 tttcaaaaga agaagcaaac acgcgcgaaa cagtcgaagt ttatcgtcgt tataaagaag
    50101 tagtacaagg aatcaccgat acagaagaac tgcttggtga aaaactggat gatgaaatgg
    50161 ctgaactagc aaaagaagaa ttatctgaat taaaaaaaga aaaagaagtc ttagaagaaa
    50221 aaatcaagat cttgctttta ccaaaagatc caaatgacga taaaaatatc atcatggaaa
    50281 tccgtggagc agcaggcgga gacgaagcag cattattcgc tggcgatctt ttcaatatgt
    50341 accaaaagta tgctgaagca caaggctgga aaacagaagt gatggaagct aacgttaccg
    50401 gaatcggtgg atataaagaa gtgatcatga tgatcaccgg agacaacgta tattcgaaat
    50461 taaaatatga aagtggtgcg caccgcgtcc agcgtgttcc ttcaactgaa tcgcaagggc
    50521 gtatccatac ttccaccgca acagttgtcg tgatgccaga agctgaagaa gtagagatcg
    50581 atattgaaga caaagatatc cgtacagata tctatcatgc ttcaggtgct ggcggacagc
    50641 acgtcaataa aacagcatct gctgtacgtt tgactcactt accaacaggg atcgttgttg
    50701 ctatgcaaga tgaacgttct caaatcaaga accgtgaaaa agcaatgaaa atcttgcgtg
    50761 ctcgtgtgta cgataagatc cagcaagaag cacaaagcga gtatgatgcc aatcgaaaat
    50821 cagcagtcgg aactggggat cgttcagaac ggatccggac gtataatttc ccgcaaaatc
    50881 gtgtgactga tcatcgtatc ggattaacta tccaaaaatt agatcagatt ttagcaggga
    50941 aattagatga gatcgttgat gcattgatca tgtatgatca aacatcaaaa ctggaagcta
    51001 tgcaaaatgg gtaaaatgac ctatcgagaa gtcctgtcac gggcttcttc ttttttagag
    51061 gatcatggga aagaagcata tagtatccaa tttttatttt tggaacgaaa acaatggaaa
    51121 aaacttgatt ggctccttca tatgaacgaa gagatttcag aagaagaaca gcggttgatc
    51181 gaaaccgatc tgcgacttct gcttgcagat catccacctc agtatttgct aggatatgca
    51241 gatttttatg atcatcggtt aaaggtaact gaagcaacac tgatccctcg accagaaaca
    51301 gaggagttag tcgaatggtg tttagacgag acgcctggtg taccgttaga agtgatagat
    51361 attgggacag ggactggagc aattgctatt agtttgaaag cagcaagaaa aaattggcat
    51421 atatctgctg tggatctttc agaagaagca ttagaagtag cgaaagaaaa tgctcagaaa
    51481 gaaggaacga aaatcagttt ttatcatgga gatacattag gaccagtaat ggatcagcaa
    51541 tttgatgtga ttatttctaa tccgccgtac atcagtcgga atgaatggga actgatggat
    51601 gaaagtgtcc ggtcattcga acctaaaatg gccctttttg ctgagaacga tggattagct
    51661 atttatgaaa aaatagcagt tgaagcacca agcgtactaa aatcaaatgg aaagattttt
    51721 ttagaaatcg gatttcgcca aggtgaagca gtaaaaaaaa tatttcagca agcatttcca
    51781 gataaaaaag tggcgataaa aaaggatctt tttggcaatg aacgaatgat ccaagtttat
    51841 ggttgaagtt gtggatgaaa aattttttat ttgttttttt attataaaaa aacacttatt
    51901 tgacaaggat gtaaacgact ttttggttta tcaacaagtt tatccactat tgacaaatag
    51961 attatccaca aacagtggaa aacttgagga taaactcaga caaaataaga atgtatgaaa
    52021 aaggggttgt ggataaactg tggaaacgaa acgatatgat gaaacagaat tgcaagaggc
    52081 agccgaagca ctgaaagaag gagaacttgt tgcttttcct accgaaacag tatacggatt
    52141 gggcgccaac gctcttttac caaacgctgt caagaaagtg ttttctgtaa aagggagacc
    52201 gcaagataat ccattaatcg tccatgtcgc ctcttttgaa caagtaaaag agtatgtgga
    52261 caactttcat ccggcagcag agaagattgt gaagaacttc tggcctggac cattaacttt
    52321 gatatttaag atcaaaaaag acactttgcc ctcagttgtg acgggaggat tatccacagc
    52381 tgcatttcgt ataccggata acaaaaaaac actagaagta atcgaacttt ccggtgttcc
    52441 gcttgtcggt ccaagtgcaa atacgtcggg aaaaccaagt ccaacgactg cagatcatgt
    52501 atatcatgat ctacaaggaa aaattaccgg gattattgat gatggagcta cgcgaattgg
    52561 tgtagaatcc actgttcttg atttaagtga tccaacagcc atgcctatga ttttacgacc
    52621 aggtgcagta acgaaagaac aaatcgaagc agtcatcgaa tctcctgttg ctattgatca
    52681 gcatcttgta aaagaaaatg aaacaccaaa agcccctggg atgaaataca aacactactc
    52741 acccgataca cgtgttctga tggtcagaga tggcgattgg tcaactgcag ttcaatgggc
    52801 aaaaaataag aagatccgtg caggtgtcat agcaagcccc gaaattgcgg atcaagtgcg
    52861 gacagatact gcagctgttt atatgtataa cgacaactca gtagaagcgg cggctaaagg
    52921 attgtttgca ggtctaagag gattagatga accaacactt ggattagatt tgatttttgt
    52981 acaagtgtat ccagaaactg gattggggaa tgcttacatg aatcgcttga aaaaagcagc
    53041 aggacaaaat tattttgaaa aatgatcaat tggttattgt cgcattcttt tagtatgata
    53101 caatgctatc aagtaaatct tctatcagtg aacgaaaaac taagcgtaac taatcgtaat
    53161 agaagagata cgaatatttg gcaatttgtt tttgatggga aaaataaaat tgtaggaaaa
    53221 taaaaaggag ctgttgtttg tggtagatta caaaacgttt gaccctgatc tttgggctgc
    53281 gatcgccaaa gaagaagaaa gacaagaaca taatttggag ctgattgcct ctgaaaactt
    53341 tgtctccgaa gcagtgatgg ctgcacaagg aagtatcttg acaaataaat acgcagaagg
    53401 ttatccaggg catcgctatt atggtgggtg tgaatttgtc gatatcgttg aaaatttagc
    53461 gattgaccgt gcaaaagaat tattcggagc gaaatttgcg aacgtccagc ctcactccgg
    53521 ctctcaagcc aacacggctg cttatctcgc tttggtagaa ccaggggaca cgatcttagg
    53581 aatggatctt tccgcaggcg gacatctaac acatggatca ccagtcaact tcagtggcaa
    53641 aacctatcac tttgttgctt acggtgttga tccgacaaca gaagttattg attataatgt
    53701 ggtacgtatt ttagctagaa aacatcaacc aaaattgatc gtagcaggtg ccagtgctta
    53761 cggacgaacg atcgactttg cgaaattcag agaaatcgca gatgaagtag gcgcaaaact
    53821 gatggtagat atggcacata tcgcaggatt agtagcagca ggacttcatc ctaatccagt
    53881 tccttatgca gatatcacaa cgactaccac tcataaaaca ttgcgtggcc ctcgaggcgg
    53941 catgattttg acaaacgatg aagcattagc aaaaaaaata aatagtgcgg tatttcctgg
    54001 tattcaaggc ggccctctag agcatgtgat tgcgggtaaa gcagtagcat tcaaagaagc
    54061 attagatcct gcatttaaag aatatagtga acaaatcatc gccaatgcaa aagcaatggt
    54121 caaagttttc aatcaagcaa ttggaaccag agtcatttca ggggcgacag ataatcatct
    54181 gatgttgatc gatgttcgcg agttaggtat aaatggaaaa gaagcagaga gcattttgga
    54241 cagtgtgaat atcactgtaa ataaaaactc gattcctttt gaaacactaa gtccattcaa
    54301 aacaagcggt attcgaatcg gcacgccagc aatcactact cgcgggttca aagaagaaga
    54361 tgctgtaaaa gtggctgaac tagttgtcaa agcattacaa gcaaaagatg ataacgctca
    54421 actagacgaa gtcaaaacag gtgtacgcga attaactgaa aaatttccgt tgcataaaaa
    54481 ataaaaattt aaattccatt agtaaacaga aaagttcaat gttgggcttt tctgtttatt
    54541 ttttgggaaa acggattctt tgacaatatc ggtgatgctc tctagtatat gaggtgtaat
    54601 tttagtcttc acactaaatc tcagggagga aaagtcatga ctgatcgtat ctcgttagaa
    54661 aaggaagcag tagcattcag tgatgcaaat gcgcctcatc cgcgtatcta tgaactagca
    54721 ccggaagaag gaagagtttt actagaagaa gtacaagaat cacctgtgaa caaatattcc
    54781 gtcgatattg aagacttgat ggtggatact ggcgaatggg gaaaactcaa tattcgtctc
    54841 gtacgaccag aagggaatat ggataaatta ccagttattt tttatattca tggagcaggc
    54901 tgggtttttg gcagtgctca aacccatgat aaattgatca gagaacttgc ggttcgtacg
    54961 aattccgtcg ttgtctttcc agaatatagt cgatcaccag aagcaaaata tccaacagca
    55021 attgaacaaa attatgctgt gttgcaacag ctgaataaat tagccaagtc aaaaaatctg
    55081 gatctcgaga atctaatcgt tgcaggagat tcagtaggtg gtaacatggc tacagtcatg
    55141 acgatcttaa caaaagaacg aaagggcctc ccaatatcca agcaattgct gtattatcca
    55201 gtcacaaacg ctgattttga tacagattcc tatcatgaat tcggagaaaa ctattttttg
    55261 acaagagaag gaatgatgtg gttttgggac caatatacga cgaatgaaga agagcgtgct
    55321 gaaatcacag cttcgccttt gagagcaaat ataaatgaac taaatgatct tccttcagca
    55381 atgattttaa cgggagaagc ggatgtatta agagatgaag gtgaggctta tgcgcgcaaa
    55441 cttcgtgatg caggggttcc tgttacacaa gtacgcttcc aaggaatcat ccatgatttt
    55501 gtcatggtaa atgctatgga tcagacaaag gcaacgcgtg cagcaatggc attatctact
    55561 tcttggatca agaaaaaaga ataagctttg cttgtccgca ggaaaacttc ttttcaaaaa
    55621 cggattttct taggaaaaat ggcgagtttg actagtcaat cacttgattt tgttttacaa
    55681 ttaaaaagaa acgacagaac aaaggagagc ttaaaaaatg ggcaaatttc aagtgatcga
    55741 tcatccattg attcaacaca aactaactat tattcgggac aaaaactgcg gaacaaaggt
    55801 ttttcgtgaa gtagtcgatg aaatcgctat gttgatggca tatgaagtat cacgggatat
    55861 gccattggaa gacatcgtga tcgaaacacc gatcactgaa acaacacaaa aaacgttgtc
    55921 tggtaaaaaa gtagcgatta ttccaatctt gcgcgcaggt attggaatgg ttgacggtat
    55981 cttggaattg attccagctg ctaaagtggg acatatcggt ctttatcgag atgaagaaac
    56041 attggaacca catgaatatt tcgtcaaact tccagaagac attgatgcac gtcagttgtt
    56101 tgtagtagat ccaatgttgg caacaggcgg atcagcaatc atggcaatcg atgcattgaa
    56161 agaacgtggc gcgtcgaaca ttaaatttgt ttgtttggta gcagcacctg agggagtaaa
    56221 agctttgcaa gaagcgcatc ctgatattga catctataca gcttctctag atgaaaaatt
    56281 gaatgaacat ggttatatcg ttcctggact aggggatgcg ggtgaccgtt tgtttggtac
    56341 aaaataagta aatacgaaaa agggtgtgac acaacttttg tcacatcctt ttttggtcat
    56401 ttttaacgtt tgatgcagaa cagctttttc acagctttcg tttgcatata aaaaaaccat
    56461 cccaaaaatg cagagatggt caaattcaaa tcttttttag ttctttgtag ttaacgatta
    56521 ctacaccctt actttgcgtc atcaagttcg cattttctgc agtttcatca atctcaacaa
    56581 cagcggaatt tttcaattgc tttgaaatcg ttccagtttg cgacttgccg tgaattgtga
    56641 acattacgtg agtattgatc ttgaattttt caccaatttc cgatggtacg atagttgtat
    56701 caaattttcc aaaagttgcc attttcataa aacctgcctt tcacatcaat tataccacgt
    56761 tttatgaaaa atatcatgaa attaaactaa ttttccatcg gactctttac aatcagccaa
    56821 atagagtata taatttcttt tggataaggc cagcttagct cagttggtag agcaacgcac
    56881 tcgtaacgcg taggtcgtag gttcgatccc tatagctggc attaaaagag aagacagata
    56941 tatcaaggat ttttaatcca ttgatacatc tgtctttttg tttaaaggaa ataaagtatg
    57001 ctttcttagc aatagagcat gtattatttc tataaaaatt taggagaata aaacgactag
    57061 caatatagct gtgaaatcaa atatcacatg aactgcccaa cttgtaatga tcgagttgct
    57121 tttaatggca gcttggttga ataacaatct tgccacccct tgtatcaaaa ggatgtgtgc
    57181 tacagtgtgt aaaatatttc cattatcata agtactgtaa tggataaatc caaagatgat
    57241 tgctgacaag aaactagcga caggaatgct gaacttacta gaaaaaatag cgagaaaata
    57301 cactgagaac aattcttctc caagtatcat gataggcaaa ataataaata ataacgggct
    57361 gaaagcctca tttattggat tgcctgttaa accccatttc aaaatttgtt gcaaaaatac
    57421 cgagactgcg gcactgatta ctacattgat aaaaaaatat ttggctatat ttttgacagg
    57481 agcgacaggt ttcgagaaca tttttttcgt atctttttta ccaaaaacaa gtgtaaatgc
    57541 tataaatcca gtgatagtta aagaaaaaat gctaataatg gatggcgcaa atagcaaccc
    57601 taacaaaccg agtacaacga caatagtgcc caatatccct tttttaagtt ctattttttt
    57661 tcctaaaatg ttattcatga taataactcc tactattcag tttttgaaga tagagtcagt
    57721 atacatgaag agttaaaata ggcaatgttt ttttatggat atttgtattg ctaaaaattt
    57781 atcttatata ttcattttga ttatactact tttatttgta aaaattttct agcaaaacca
    57841 agtgcttgca actgattaag cgtacaattt tttagatgtt ccgagcgtac ttcaatcatt
    57901 tcaaaggagt tgctacttaa atcgatattt tttaaatcag tatcaaaaaa gctagcattt
    57961 tctaaatcat tagtagaaaa cattatttta gtgagggaac agtctacaaa atctgcgctt
    58021 gataagacag agtgctcaaa ggtactttct tttatgtttg aaccagataa atttacatag
    58081 tccattttac aattctcgaa taacgtgttc ccaatataac tttccatcaa tttagtacct
    58141 gttaatttac aattgacaaa tttcacgcga tgcaacatgc tttttgtcaa tatacagcct
    58201 gaaaaatcac agttttcgaa tatacagtcg gtaatctcca ttctttgaaa atcattggaa
    58261 gcaaaaaatg catacttgaa aatagatttg taaataattg gatggcagat tatttggttt
    58321 tcgatgacct ctttttgtga aaggatatta tacagttcaa aatcttcgtt ttcatagata
    58381 tgaaaaaagg atcctttatt caagcttgct tcattaactt taggaaaaat catatttaat
    58441 tacctccatt ttcataaagt catattaaca aaagctaaac caacttacat tagcagaaaa
    58501 tgagataggt tttcgccttt ttcgataacg atagtgtcgc tatctcttga aacgttctta
    58561 gaattgccct gagagacaca aattaggcgt aattctaata aaaatcttag aataaaaatt
    58621 tttactgatt taatttagaa tgtttttaaa ataaaaatcg ttgccataac agtgtattga
    58681 aaggttttaa caatccagta atgatttaaa aaaatctaga tgtagcgtat actttgttta
    58741 tagaatgtag tgggaagaaa tatatttttg ggaggaaatc gtgatgtcta aaagtgaaga
    58801 aatagaaaac atggtactaa aattatatga tctcgtttta aatccagaga tcaaagaaaa
    58861 ggaacgtata cttttgatag acgcaaagac aggcttagaa aaaggtcagt attatcctaa
    58921 agtcataaat aatttggaac gtagtttacg tccgttagca atcaggggag aattgtctaa
    58981 accagtatca ccgttttata tggagatatc aacaatagga aaatttgaaa aagaattagg
    59041 tcgaggaatg gcttcggcac cgattacttt tgggcattta taaaaacgag ttattcatct
    59101 caatgaatta agaagtatag tcaatctcat aatgcaggct aggttcattt atttgaacag
    59161 agtctgtatt tttttattct aaataaagaa acagatgttt ctttatattg tattttatcc
    59221 tttaattaac acaaaaacag ctagtataaa ctaataatgt taattaaaag tgta
//
LOCUS       DAMYXM010000012        59061 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.012, whole genome shotgun sequence.
ACCESSION   DAMYXM010000012 DAMYXM010000000
VERSION     DAMYXM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.012"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(59..187)
                     /locus_tag="PNS21_000849"
     CDS             complement(59..187)
                     /locus_tag="PNS21_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDI5158579.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="PNS21_000850"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="PNS21_000850"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDI5158580.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="PNS21_000851"
     CDS             complement(1674..2729)
                     /locus_tag="PNS21_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDI5158581.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="PNS21_000852"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="PNS21_000852"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDI5158582.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="PNS21_000853"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="PNS21_000853"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDI5158583.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="PNS21_000854"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="PNS21_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDI5158584.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="PNS21_000855"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="PNS21_000855"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDI5158585.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="PNS21_000856"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="PNS21_000856"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDI5158586.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="PNS21_000857"
     CDS             10491..12050
                     /locus_tag="PNS21_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HDI5158587.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="PNS21_000858"
     CDS             complement(12161..14092)
                     /locus_tag="PNS21_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI5158588.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="PNS21_000859"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="PNS21_000859"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDI5158589.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="PNS21_000860"
     CDS             complement(15021..15773)
                     /locus_tag="PNS21_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDI5158590.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="PNS21_000861"
     CDS             complement(15932..16141)
                     /locus_tag="PNS21_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158591.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="PNS21_000862"
     CDS             complement(16152..17480)
                     /locus_tag="PNS21_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158592.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="PNS21_000863"
     CDS             17616..17771
                     /locus_tag="PNS21_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158593.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /locus_tag="PNS21_000864"
     CDS             complement(17845..18432)
                     /locus_tag="PNS21_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDI5158594.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="PNS21_000865"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="PNS21_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDI5158595.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="PNS21_000866"
     CDS             complement(20012..21301)
                     /locus_tag="PNS21_000866"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDI5158596.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="PNS21_000867"
     CDS             complement(21455..22387)
                     /locus_tag="PNS21_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158597.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="PNS21_000868"
     CDS             complement(22544..23203)
                     /locus_tag="PNS21_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDI5158598.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="PNS21_000869"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="PNS21_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDI5158599.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="PNS21_000870"
     CDS             complement(24244..24534)
                     /locus_tag="PNS21_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158600.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="PNS21_000871"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="PNS21_000871"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDI5158601.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /locus_tag="PNS21_000872"
     CDS             complement(26821..28176)
                     /locus_tag="PNS21_000872"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDI5158602.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="PNS21_000873"
     CDS             complement(28200..29369)
                     /locus_tag="PNS21_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158603.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /locus_tag="PNS21_000874"
     CDS             complement(29366..30253)
                     /locus_tag="PNS21_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDI5158604.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="PNS21_000875"
     CDS             complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="PNS21_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDI5158605.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34320..34841
                     /locus_tag="PNS21_000876"
     CDS             34320..34841
                     /locus_tag="PNS21_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDI5158606.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35540..36105)
                     /locus_tag="PNS21_000877"
                     /pseudo
     CDS             complement(35540..36105)
                     /locus_tag="PNS21_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36284..36940)
                     /locus_tag="PNS21_000878"
     CDS             complement(36284..36940)
                     /locus_tag="PNS21_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158607.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="PNS21_000879"
     CDS             complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="PNS21_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDI5158608.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38477..39475)
                     /locus_tag="PNS21_000880"
     CDS             complement(38477..39475)
                     /locus_tag="PNS21_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDI5158609.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="PNS21_000881"
     CDS             complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="PNS21_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDI5158610.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(40491..41222)
                     /locus_tag="PNS21_000882"
     CDS             complement(40491..41222)
                     /locus_tag="PNS21_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HDI5158611.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(41226..42491)
                     /locus_tag="PNS21_000883"
     CDS             complement(41226..42491)
                     /locus_tag="PNS21_000883"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HDI5158612.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="PNS21_000884"
     CDS             complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="PNS21_000884"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDI5158613.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="PNS21_000885"
     CDS             complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="PNS21_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDI5158614.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            47833..49335
                     /locus_tag="PNS21_000886"
     CDS             47833..49335
                     /locus_tag="PNS21_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDI5158615.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            49337..50074
                     /locus_tag="PNS21_000887"
     CDS             49337..50074
                     /locus_tag="PNS21_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDI5158616.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="PNS21_000888"
     CDS             complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="PNS21_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDI5158617.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="PNS21_000889"
     CDS             complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="PNS21_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDI5158618.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="PNS21_000890"
     CDS             complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="PNS21_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDI5158619.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="PNS21_000891"
     CDS             complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="PNS21_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HDI5158620.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(55574..56740)
                     /locus_tag="PNS21_000892"
     CDS             complement(55574..56740)
                     /locus_tag="PNS21_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDI5158621.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(56756..56983)
                     /locus_tag="PNS21_000893"
     CDS             complement(56756..56983)
                     /locus_tag="PNS21_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDI5158622.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            complement(57370..57626)
                     /locus_tag="PNS21_000894"
                     /pseudo
     CDS             complement(57370..57626)
                     /locus_tag="PNS21_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            complement(57969..58992)
                     /locus_tag="PNS21_000895"
                     /pseudo
     CDS             complement(57969..58992)
                     /locus_tag="PNS21_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
BASE COUNT        16276 a        12803 c        10274 g        19708 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat tgatatttaa
    27541 cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatt
    29221 ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc agttctctac
    29461 gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta tctaatgtaa
    29521 tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata tctgcatcta
    29761 atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata gtaattagcg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca taattttcag
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg tcgttttttc
    30541 ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt
    30601 ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cttggatagt
    30661 cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg aaaagcgaac
    30721 ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat
    30781 tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc aatagccaga
    30841 agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca aaccatgtgt
    30901 gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg cttcttcaaa
    30961 agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc
    31021 atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa
    31081 tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac
    31141 ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat gatcgatccc
    31201 gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac
    31261 gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc gttcgctttc
    31321 gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa acatgtgtac
    31381 caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat
    31441 tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc
    31501 agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt
    31561 ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat
    31621 catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc
    31681 tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt
    31741 ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc
    31801 ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc
    31861 ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac
    31921 gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc
    31981 ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca
    32041 catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat
    32101 agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac
    32161 agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt
    32221 gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac
    32281 ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagccat
    32341 tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa
    32401 aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc
    32461 caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc
    32521 agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag
    32581 agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag
    32641 gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat
    32701 cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata
    32761 ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt
    32821 atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg
    32881 ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat catccacgat
    32941 ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc
    33001 tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc
    33061 cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc
    33121 tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt
    33181 ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt
    33241 ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat
    33301 agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt
    33361 tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata
    33421 ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact
    33481 aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt
    33541 gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt
    33601 acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag caagatgggc
    33661 aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt
    33721 ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac
    33781 tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt
    33841 cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg caccagtttt
    33901 caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac
    33961 tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta attcagccat
    34021 ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata
    34081 tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc
    34141 atattctctc ttattatctg ttccattata acgtaaaaca ttccattttt tcttttattt
    34201 ataaaaatat ttcacatatg tctatttgaa ttggtcatct aatttttaga caaacagtct
    34261 aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg tgttttaaca
    34321 tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc ctttatggtt
    34381 catcgaatgc tcctaaaaaa atggtcgaat tcatcaacct atcttgtccg tattgccgac
    34441 aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg caattgcagc
    34501 gagtgatcaa attgttcgat aaagaaaaag aaagcttgca gcgaggaaac gtgatgcacc
    34561 ggtatctcac aatcagcgat ggtcaaaaag cgatcaaaga gatcaaacaa atattcgata
    34621 ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca gtagataaac
    34681 taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac gaagctgagc
    34741 aagcacatat tcgatttgtt ccgactgtca tcttgggtaa agaaatcttt gatgaatcta
    34801 tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag gagcgtagga
    34861 caaaagtaaa gaacactttt gtctcacgct ctaaacacct ataaacggcg gaagcagaag
    34921 caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta ttccgaataa
    34981 atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa gctgagcaaa
    35041 ttatctccac caaataattt attcatattt ttgacttgcg catactaact aattcttaaa
    35101 ttttcataca aaatttctca aattccctta acgagtaata tttatgtacg gtgattcaca
    35161 ctagtatccc aatcactttt taagttatcc cgatttcaat tctacttcaa aaatagttgc
    35221 ttttatgtat gctgttagtt gattagatgg tcgtttgttg agctaggtcg tttattttaa
    35281 acaagttgtt tctttagctt atacctatct cgtctaccaa acgcaagatg gcaaacacaa
    35341 atgcatacgc atctgtattt gccactttga ttcgtataaa gctaacaact cctaactcct
    35401 ctcatttaat agttgctctc ttttttattc agatacattt ttgctgatca cagcctagta
    35461 tttcaatggt tttgaacact aaaccaaatt gttccattac aacattcact ggctgatgaa
    35521 atggattaat agatttcgat tattcaaagc gattttctat tggtggattg tgttcccaac
    35581 taacaagacg gattccaaag tcattatata actcttcaag agttaaccct ttcctgttcc
    35641 caatagatgt atctctccca cgctcgatat aaaatatgga gtttcgaatg ccaagacctg
    35701 gcaaatcttt ccttgttaca gttacactgc tactaacttc cccatataaa atccagctcc
    35761 tccaaaatga taatatattt ttcgaccatc atctaactca tgtaacaata attgttgtgc
    35821 cctcttgtca ctcgccaaat gcaaacgcca cgtgcgatac catgccatgc tacctgcatc
    35881 tgcagaaaat ccatcaaata atgcaacaat tgtatcttct atcacaatcc tttccccatc
    35941 tacttcatat tctaatctaa aatcaaactc ccctcgggta atcgcaggtc ttggtggctt
    36001 aggacccaat attattctac ttaaaaccgc attcgttccc agatgaaaca cagctataac
    36061 aacaataatg attaatattt tgtttctatt tgataaattt ctcattattc gcaaatcacc
    36121 tctacattct cacatactag ttcgtaatac tcctttaata tcaaaatatc tactgatcgt
    36181 aataatattc gctatgaatt cctactactc gtataagaaa ggccaaatca tcagctttta
    36241 cttgctgatg atttggcttg tttctattta tcgtaattgt tttctaatca ccaaaattgt
    36301 taacaatcgg ttcagatatt tcccaattca ctagccttat attatattga tttaacaatt
    36361 cttcttcatt tattgtattt tgatcaagcg gaccaaattg ttctaataat tctttgtcat
    36421 gtaaaatatc ccttctagta atgcctcttt ccctttcaat gaaagaagca ctaggaaaag
    36481 tatgattata accattcaac ctatctccca tgtaataatt agcaggtcct ggaaaataaa
    36541 atatttgctt attatcacta acttctaaca gcaacactaa atcttctccg ctggctaaac
    36601 gacttgtcca tctacgatat ctccctatgc cttcgttcat tccaaatcca tcaaattcaa
    36661 ctattaacgt gtcttctatc acatgtaatt cttcattcaa ttcatactca agtctaaaag
    36721 gaaattctcc atatgtaatc tctggcaatg gtggtgctgg agttctccaa ataaatacaa
    36781 taactgtgaa tagaaaaaat ccaacgatcg atataaatgt tatgagacct gctcgaagta
    36841 aaacccgata taataaaaat gctgtatttc gctttgctat atctagaaaa ataaaaagta
    36901 ctgcaatcat aatgatagta aaagttgtaa aaaaacgcat ttttgccctc cctttataaa
    36961 catcgattct caaattctaa accaaatctt ggtagttcaa gttcaggtat aacgtattcc
    37021 aaccatacat ctcaattact atttatgaac ttatgattat cactgaataa atcactccat
    37081 ccccttgtta aaacttctac attaattaca cctggttgtt ttcgactagc taatccaatg
    37141 aacttatcaa ccatccttat ccaccaacca cgagaatctt gaccagaagt aagaagagca
    37201 gttctcaagg atgatcgagc agttccttcc agttttttaa ttaatcaggc cagttataga
    37261 taaatacttg atcttatcta tatcaggact tctatattaa gcaaaaattt atgtacaaaa
    37321 ctaaaatctt cattcagttg tctccccgct ttttatacga ttgtattaaa aacaatagaa
    37381 gcctttccaa attatgttac tccagtaaag atggaattat agtttgacgg aagaaaagca
    37441 agtataactt ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta tcagtttttt
    37501 ctttggatta aactgcttct tcacgcagtt tttccgcaaa ttcatttatt ttgcgaattc
    37561 ggtgattaat tcccgatttt gatatcgcac ctgagggaat catttcccct aattctttca
    37621 agctgacctc tggatgttga agccgaagtt ctgcgatttc ctgaagtttt tcaggaaggg
    37681 catgtagacc gacctttgct tcgatcaatt cgatgttttc gatttgttta gaagccgcat
    37741 caatcgtctt gttcagattt gctgtctcac aattgactag acgattgacc gaatttctca
    37801 tatcccgtac gattcgaaca tcttcaaatc ggagcattga gttggttgca ccaatcaatg
    37861 ttaagaaatc agcaattttt tctgctcctt ttaaataaga aatatagcca ttgcgccgtt
    37921 caagcgttcg agcatgtaga ccgtaataat ttaacatatc gcaaatgtct tggttatgct
    37981 gttcataaat agaaaatatt tccaaatgat aacggctcgt ttccggatta ttgactgaac
    38041 ctgaggccat aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt cccataatct
    38101 cgtccgaaac gtgtccattg aacatcatac catccatgat atcaagatcc atcaacacat
    38161 cttttgtgcc ttgcttcaac cggacgatat agacattgtt cttttttaat ttcatttttt
    38221 tacgaacgag tagctcactt cgtacctgat aatgatcttt caaaagagaa tagatacgtc
    38281 tggcaattgc agcattttct gtttggacgt tcaaaataaa ctgctggttc acaataccta
    38341 aagagccgtt cattcgcagc aaagcagcta attccgcttt cgcatgttct cgatgaactt
    38401 ccaaaccagt tagttctttc ttcacatctg cagcaaatga catcctattc tcctcctttc
    38461 tccaacaata aattgcttaa tattttgaac cgaaaactaa acggaacaat tcttctacta
    38521 ctttgtctcc atcgtggaag acaccaccat ctttcaattc caaaaagtcg gtggaaatca
    38581 ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc gacttgtact agatactcat
    38641 catagatctc tgggtccata taatcttcag gcactttttc cgtattgacc aatactgtat
    38701 cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat atgatcggca tctgtgaaat
    38761 gttccgtttc ccctttttgg gtcatgatat tacagatata gacggtttca gcagttgtct
    38821 tgagcattgc ttcgccgatt tctggtatca ctaaatttgg aaggatactg gtgaataaac
    38881 ttccgggtcc caaaacgacc atgtcggcgt ccataattgc tgaaacgact tttcgagccg
    38941 ctttggcttc acttttatca tttgtgttgc agacatacac acggtcaatg gttttacggt
    39001 cgatcgcgat tttagattct cctactgcag tcgttccgtc tttaaagaca gcatgcaagg
    39061 tcaaaggagt ttctgaagaa ggataaacat gtccatctac atgcatcatt tttgctaata
    39121 attgaatcgc ctcataagtg cttccccgca tctcggaaac tgcggcaatg atcagattac
    39181 cgatcgtatg attggctaaa aactgatctt cctttttgaa acggtattgg aaaatatctt
    39241 catagaattg tggcatatcc gataacgcca ctaagacatt tctcaagtca ccaggaggag
    39301 ccatagcgat cgattctctg attgcaccgc tgcttccccc atcatctgca acagtcacta
    39361 ctgctgtgat atctgctcct tgatttcgca aacttttcag gataactggc agaccagtgc
    39421 caccgccgac cactacgatc tttggttttc ttatccggta ggttttcatt ttcacgaccg
    39481 gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc acattgactt tatagttttt
    39541 ccctaaagca tttgccaaac gttcagtcaa tgccacagaa cggtgttgac cgcctgtaca
    39601 gccgattgca actgtcaagc tgctttttcc ttctttaatg tatccaggca atacagtttc
    39661 cagaagatcc aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca tcacataatc
    39721 atagactggc tgatcttttc cggtcaatgg tcttagttcc gggatataat gagggttggg
    39781 caaaaaacgc acatccatca caatatccga atcgatcggc aacccgtatt taaaaccaaa
    39841 tgatacaaat tcgatccgaa atccttggtc tccctttgat ttgaatgact cattgatttt
    39901 ttcacgtaat tgtcgtggtg tcaatgtcgt cgtatcaatg atcacggaag cttgggcttt
    39961 caagtcgtct agtatcgccc gttctttacg tatcccttca gtgatcaaac cgtccatcgc
    40021 cattggatgt gctcgtcttg tttctttgta gcgagccact agttcttcgt ctgaagcatc
    40081 taaaaataag atagaagtgt cgatgaagtt cgtattttcg atttcgatca gcatgttttg
    40141 gatttcttca aagaaggaac gagaacgtaa atccactcct aaagccattt ttgtgacttt
    40201 gcctgattct ttgattaatt cccagaattt tggaatcaat cgaggcggca tattatcgat
    40261 acaaaaatag cccatatctt caaaactttg gatagctact gttttccctg caccgctcat
    40321 acctgttatg atgactaatt gcaaattatc tgtcataatt acgaactcct ctcacattgt
    40381 gcatagtaaa attatatcat tccgggcgta tcattaccag ttttcttttt cttttacaaa
    40441 aagatttgga ccaaatatct tttaggatat ttgccccaaa tccaattttt ttacttttcc
    40501 gatgctgtat ccaatgcctt gcttctttca gctagcgctc gttcaatcgt ccgatctgct
    40561 aaaatagatt gggcatcgat cactggataa tggttatctt cagcaaattc atgcatcaat
    40621 gacaattctg tgcaacctaa gataactttt tcacagttta ggcgctctac cgcttcttct
    40681 aagatttcgt aatatagctc ttgattcaaa taatccgatt ccttgatctc atgatagatc
    40741 agataattga ttttttcttg aagaacagta tcaggaatca ctgtttcgaa tccaagattt
    40801 ttgacttcgc gttcatagat tccagctttc atacttcctt ctgttcctag aatcgctact
    40861 ctccctgtcg tatgctgacg gacaagttca tttgctgctt ctcttggcat atgaaggata
    40921 ggaatatccg ttgccgcttg taattcttca aaaaagtagt gtgcggtgtt acaagtcaac
    40981 acaatgaagt tcggctgcaa taaattttgt ttctcaatat catccagcag aaacggcatt
    41041 ggattttctt ctgatcgatc taaaatataa gctgtacgat caggaactgt tgcatgattg
    41101 aataagacat aattcaaata ttcttgatct tttgtagctt tcgttcggtg gttgatcaag
    41161 cgaacaaaac tttctgtcgc catcgtgccc attccgccta aaatactgaa aaaattctcc
    41221 atgaactagc cttctttcac atgaaaatac tttttgaatc ttggaatata gttatggaac
    41281 atatatttca tcaacaacca gcgcttgatt gaccggtctt tttcataaaa cacagtagta
    41341 ccgtaacgat tttctttgat catttgctca gctagttttt tgtcttcatt ttcccgagca
    41401 tattctaaaa agattttttt gggcacaccc atccataatc ttgctttatc tgattgattc
    41461 gttccatagg tcgtttcaac aaagggtttg ttaaacactc gatcttctgt gacgaaacga
    41521 gccagattca agccgttcaa tgtcacaaag aaactgctac gaccttgtct caaattgatt
    41581 tcaaataatt tatattctcc atctctagga tcatacttca tatcgaaatt ggcaaagcct
    41641 gtatattcga ttttttccaa aaatgcctta atggtttgat agattttttc gttataatct
    41701 ggcatgatca caacgtaatt accaatcgat gctggtgtag gatcttctaa cagtggatga
    41761 ccaagacaca tcatccgaac ttgatgatct tcatctacat acgcatttag cacacgcata
    41821 ttactatcat cacccgggat aaaatcttgt acaatcattt cacttttata accggcttcg
    41881 tatatccggc ctaaaattaa atcgaactct tctctagtct ctaaaataaa agcttttttt
    41941 cttccttcaa attggacaga aagatactcc acactgtttg caggtttcaa agcaactggg
    42001 aattcaaaag gcaattcctg ttccaaatgt ccatttacta acatttcttc tctgacgatc
    42061 aatgttttag gatacggaag atcatattct tcacaaactt cataaaagct tactttattg
    42121 atcagtcttt caaataaaga ataatcaata tatggacaga taaaatattc tgacagctct
    42181 tgtttatgtt gggagatcaa ttctgcatat ccatcaccac aagcaatcaa aagatatttt
    42241 ttactcttat cagaatatct ttcttttgcc aaacgaagca tcgtctcgat aaacacaggg
    42301 tctttatcaa atcctggaat aacttctaca ttgacaattt tactgtagcg ggtaggggct
    42361 aactgatccg aagcatatgc ttggcagatt ttgccgtatg cttcattaaa agaacgtgcc
    42421 ataccataaa cgttcatgtc gcttcctaat aatataggga taaattcttc attttcaata
    42481 ctgttcatca ttatctctca cttactcata atctcttgtt tttcttttcc aactacacaa
    42541 ggaatatcat accataaaga aaaaagcaat caaaggaaac tcctccgatt gctcaattct
    42601 tttttgaacg gattgaactt ataggaagtt tttgtctata ccaatcagtg tagaaagaac
    42661 taccatgtaa tgattctaga acactgatat taataaaaca agcaaaaatt cattttttta
    42721 tgttttttta actatctcag ctatctatcc tctttatacc attatttttt tcaaatagtg
    42781 acctgtataa ctttctttta cgttaatgat ttcttctggt gttccagtcg caaggatcgt
    42841 tcctccacct tctccacctt ctggtcctaa atcaatgata taatctgctg ttttgattac
    42901 atctagattg tgttcaatca ctaaaactgt gttaccagca tctactaatc tttgtaatac
    42961 atgcaacaag cgggcgatgt catcgctatg aagtcctgtc gttggttcat ctagtatata
    43021 gaaattcttt ccattagaga ttttgtgcaa ttcactggca agtttcatcc gttgtgcctc
    43081 accaccggac aatgtcgttg ctggttgccc catcgtcaca taacctaagc caacatcaac
    43141 aatcgtttgc agtttgcgat gaatctttgg aatgtgcttg aagaattcta cagcatcttc
    43201 tactgtcatt tccaaaatat cagcaatgct ttttcctttg taatgcactt ctaatgtttc
    43261 agagttatat cgtttgccat gacatacttc acaaggaaca tagacatcag gcaaaaagtg
    43321 catttcgatc ttaataattc catccccgcg acaagcttca caacgaccgc cttttacgtt
    43381 gaaactaaaa cgccctttct tataaccccg catttttgcc tcgttcgttt gagcaaataa
    43441 cccacggata tcatcaaata cacttgtata agtcgctgga ttacttctcg gcgtccggcc
    43501 gattgggctt tgatcgatat cgatgatctt ttcgatactt tcgtagccgg aaattgtctt
    43561 gaatttacct ggcttagcag aattcttatt taatttttgc gctaacgatt tctttaagat
    43621 actattgacc aatgtactct ttccagaacc agacacacct gtgactgcta caaattctcc
    43681 taatggaaat tcaacactaa tatttttcag attgttttct gaagcaccag tgatcttgat
    43741 tgctttccca ttacctttac ggcgttcttt tggtacaggg attgattttt tccctgaaag
    43801 atatttccct gtcaaagaat tttcatcatt agctacttct tcaggagtgc ctgcagccac
    43861 gatttgtccg ccttgttctc cggccccagg tccgacgtca atcagataat ctgctgccca
    43921 catcgtatct tcatcatgtt cgactacaac gagggtgttg ccaagatcac gcattttctt
    43981 taatgagcca atcagtcgat cgttgtcgcg ttgatgcaat ccgatcgatg gttcatctaa
    44041 aatatacaaa actccagaaa gattcgatcc aatttgtgta gccaatctga ttcgctgcgc
    44101 ttctccccca gataatgttc cggaagcgcg actcaaagtt agatagtcta gtcccacatt
    44161 ttccaagaat gaaagtctgt catttacttc ttttaaaatc ggtttggcaa ttactttctc
    44221 ttgttcagat aacgttaagt ttgagaagaa atctactgct ttttgaatag acaactcatt
    44281 tgtttgcccg atattcgttc cattgatttg aacagataga gcttggggat tcaatcgata
    44341 acctttacat gtctgacaag ttaattctgt catataaaga cgcatttgct cacgcgtgaa
    44401 atcactgttc gtatcatgat aacgacgctc gatattcttc aatacccctt cgaaagtcgt
    44461 ttctacatcg cgaacgccac caaaatcatt ttcataatgg aaatggaaaa gatcatctgt
    44521 cccattcaag atgatatcct gatgctcttc cggtaattct tcgaatggtg tatctaaatc
    44581 gataccgaaa ctttcacaag cctgcgctaa catttgagga taatactgag agctgattgg
    44641 attccatgga acgatagcac cttgactaag tgtcttcgtt ctatctggaa tcactaaatc
    44701 ttgatcgact tctaatttga cacctaaacc atcacagtct ggacaagctc caaaaggcgc
    44761 attgaatgag aacagtcttg gttctagttc gccaacagta aatccgcaat aaggacaggc
    44821 atagtgttcg ctaaacaaca tttcttcttc accgatcacg tcaaccagag catagccgtc
    44881 tgccaatcgc agagccgcct ctaaagaatc aaatagacga gaacgaatcc cttctttcac
    44941 aatgatccgg tcgatgacga tcgcgatatc atgttttttg tttttctcca gctcaggcac
    45001 ttcacttaaa tcatagatct caccatcgac tcgaacgcgg acgtatccct ctttttggat
    45061 tcgttcgaat atttttttat gttgtccttt tttctttacc acgatcggtg ccaacacttg
    45121 gatctttgtg cgctcaggca aagccattac ttgatctgcc atttgatcta ctgattgact
    45181 ggtaatctcg atatgatcgt tgggacaaat cggatgtcct acacgtgcaa acaacaagcg
    45241 taaatagtca ttgatttccg tcacagtacc tactgtcgac cgaggatttt tacttgttgt
    45301 tttctgatca atagaaatcg ccggactcaa tccgtcgatg ctatctacgt ccggcttatc
    45361 catttgtcct aaaaactgac gagcataagc agaaaggctc tctacgtatc tccgctgccc
    45421 ttcagcgtac agcgtatcaa atgctaggga actttttcca gaaccggaca aacctgtaac
    45481 gaccacgaat ttatctcgtg gaatcgttac atctatattt ttcaagttat gggcacgtgc
    45541 accgtgaatc gtaattttat cgttagccat gttgtcctcc tactttgaag cttttaattc
    45601 taaaatcata tctcgcacat tagcggctgt ttcaaaatcc aatgctttcg ccgcattttt
    45661 catttctttt tctaatttca tcaataacgt atctttttct tctttcgtca tttcttcata
    45721 cgagacttgt accgcttctt ttgtatcatc ttccgattct ttcgtaatag aaatcaaatc
    45781 tcggatttct ttaatgatcg tttttggaac gatcccatgt tcttcattgt acttctgctg
    45841 aatcgtacgg cgtcttgatg tttcatccat cgctagacgc atagaatctg ttactttgtc
    45901 tgcgtacatg attactttac cttcttcatt ccgagcagct cgtccaatcg tttggactaa
    45961 ggaacgttca ctacgtaaaa agccttcttt atccgcatcc aagatagcaa caagggaaac
    46021 ttcaggtacg tctaatcctt cacgaagcaa attgattcca actaacacat caaattcacc
    46081 taatcgaaga tctcgaatga tttctgttcg ttccagcgtt ttgatatcac tatgcaaata
    46141 tttgactttg atccctaatt ctttgaaata atctgtgagg tcttctgcca tttttttcgt
    46201 caatgtcgtt acaaacaccc gctggtcttt ttcgactcgt tcatttattt cgcctactag
    46261 atcatcgatt tgtcccatga ttggacgaac ttcgatcact gggtcaagca gccctgtcgg
    46321 acggatgatt tgttggataa cggtatctgt ttgttcatgt tcatatggtc ctggcgtcgc
    46381 agagacataa atgatctgat tgacatgttt ttcaaactct tctaaccgaa gtggccgatt
    46441 gtctaaagca gatggcaaac ggaaaccgta atctactagc atctgttttc tcgctcggtc
    46501 accattatac attcctctga cttgaggcat ggttacgtgc gattcgtccg ccacgatcaa
    46561 aaaatcttca ggaaagaaat ccaatagcgt ataaggcggt tcaccttctt tacgtccgtc
    46621 catatgccga gaataatttt caattcccga cgtgtagccc atttctaaca tcatttcaat
    46681 atcgtaattg gttcgctgtt ctaaacgttg agcttccaga agtttgtttt catttcgcaa
    46741 gacagttaaa cgctgttcta attccgcttt gatattcgct acggcatgct ccatatgttc
    46801 atcattcgtc acaaagtgag tcgccgggaa gattgccacg tgttccgttt ctcctaaaac
    46861 ttcacctgtc agtgcattga cttctcggat acgttcgacc tcatctccaa aaaactccac
    46921 acgtaaggca cgttcatctc ttgaagccgg aaaaatctcc acgacatcac cgcgcactcg
    46981 aaaacgaccg cgctgaaaat ctatatcatt tctttcgaat tggatactga ctaagtcacg
    47041 aatcaactga ttgcgatcta gttctgctcc ctgtcgaatc gaaactactt gtttttggta
    47101 ctcaaaaggc gaacctaatc caaaaataca tgaaacggat gcaatgacga tcacatcatt
    47161 tctttctaat aatgaactag tagccgaatg tcgtaatttg tcgatctcat catttacact
    47221 tgaatctttt tcgatatacg tatcgcttga aggcacataa gcttcaggct gataataatc
    47281 ataataagaa acaaaatatt caacagcatt gttagggaaa aattccttga attccccgta
    47341 cagctgacca gccaatgttt tgttatgggc aataattagt gttggtttat tcactttctc
    47401 aataagattg gaaatcgtat atgttttccc tgttccagtc gcacctaata agatttgtgc
    47461 ttttttcccg ccgactacgc catccactaa ttggttaatc gcttcaggct gatcaccagc
    47521 cggttggtat ttcgagacaa gctcaaagtg tcgggaggta tctctctcaa tcattcttcg
    47581 tcctcctttt cattcattcc ttttcatttt acgaccaata aaaagaaaag gaaagcaaag
    47641 gcattcctta ctaaaaaatt ataccacacc gaacatactt tcgccacttt tttatcttat
    47701 tatgcatgtt acgttttata acataaaatg ttagaaactc gcattttttt cattttttcg
    47761 agcttacagt cttgtctgat tcagcacttt tcgcctataa taataaaata ttggaaattt
    47821 aggaggttga ttatgaaaag aaagcattta tttcactcat ttgttttctt aatgacagga
    47881 cttgtgacac ttgcgtttgg acatgttgca caagctgaca acactacaac ggcagagtca
    47941 agcacaagcg agacaatgca aaaaatagag cccaaaaaag acgtatacgt aattgccagt
    48001 gactctgctt ttgctccatt tgaatttcaa aacgcaaagg gcgagtacga aggcatcgat
    48061 gtaaaattga tggaaaacat cgccaaactg caaggattta acatccgaat ggactaccgt
    48121 ggtttctctg ctgctttgca agctttggaa tcaggacaag cagacggaat gattgccgga
    48181 atgactgtaa cagatgaacg aaagaaaagc tatgattttt ctgattctta ctttgatagc
    48241 gggatccaat tagctgtcaa aaaaggagat acttcgatca agtcttatga agatttgaaa
    48301 ggaaaaacag tcggagcaaa agttggtacc gaaagtgctg aatttttaag cgaccatgaa
    48361 tctgaatatg gctattctat caaatatttt gatgctgctg atcaattgta cgacgggtta
    48421 aaagttggcg caatcgatgc aatgatggat gattatccgg taatcggtta tgcgatcaaa
    48481 caaggacaag aattggaaac accgatcaaa aaagaatctg gcggaaaata tgcatttgcc
    48541 gttaaaaaag gacaaaatcc tgagcttctt aatatgttca atgaaggatt gaaagaatta
    48601 aaaagaacag gcgaatacga taagattatt aaagaatatg tagcggatgg ttcagaaact
    48661 tccagtgagt cttccactgc tgatgaatca acatttcttg gacttatcca aaacaactat
    48721 caagcattgt tgaaaggact ttggagaaca attctcctaa ctttgatctc ctttgctttg
    48781 gctatagtaa tcggtgtgat ttttggatta ttcagtgtag caccaatacg cgctcttcgc
    48841 acaattgcta gtatttatgt cgatatcatc cgaggaattc caatgatggt tcttgcgttc
    48901 tttattttct ttgggttgcc tggtatcgtt ggatttacga ttcctgactt ccttgctggt
    48961 gttatcacct tgactcttaa cgcaagtgct tatatcgcag agattgttcg aggcgggatc
    49021 aacgccgttc ctgtcggaca aatggaagct tccagaagtt taggtctttc ttataatcgg
    49081 acaatgcaaa aaatcatttt gccgcaagcg atacgcatca tgatcccatc attcgtcaat
    49141 cagttcgtta tctcattaaa agacacaacg atcatttccg caatcggtgt agtcgaattg
    49201 ctgcaaactg gtaagatcat tgttgcacgt acaacacaaa gtacgtatgt ttacttgatc
    49261 attgcgatca tgtacttgat tttgatcaca gcattgacaa aattagcaaa agttttagaa
    49321 aagaaggtga agtaagatgg cagaaaaaat cttagttgag catttagtca aaaaatacgg
    49381 cgataacaca gtattaaacg acatcaatgt gtccatcaac gaaggagatg tcgtctgtat
    49441 cattggccct tcaggttcag gaaaaagtac atttttgcgt tgcttgaatc gtttagaaga
    49501 accttctgac ggtgaaatta ttattgacgg cgcccacttg atggataaaa gcacagatat
    49561 caacttggtg agacaacata tcggaatggt attccagcac tttaacctat tccctcatct
    49621 ttctattttg gaaaacatca ttttagcacc gatggacctg aagaaagaat ctagagaaga
    49681 agcagagaaa aaagcaatca aattgctgca gacagttggt ctagaagata aaaaagatat
    49741 gtatcctgaa atgctttccg gtggacaaaa gcagcgtgtg gctattgctc gtgctctagc
    49801 gatgaaccct gacatcatgc tttttgatga gcctacatct gcactcgacc ccgaaatggt
    49861 cggtgatgtc ttgaacgtca tgaaaaagtt agcagaacaa ggtatgacga tggttatcgt
    49921 tacgcacgaa atgggcttcg ctaaagaagt ggccaatcgt gtcatgttca ttgacgacgg
    49981 aaacttttta gaagacggaa cgcctcaaca aatctttgaa aatccgcaaa atgaacggac
    50041 aaaagatttc ttgaataaag tattaaatat ttaataaaaa ccaaaaatgc ccggtcgctt
    50101 catgacgacc gggcattttt agtttcttgt tttttcactt aactgtacca gccgcatcgc
    50161 ttgttctcct gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat
    50221 cggttcattg acgatatcaa gtacatagtc gataccacct tcagaaacta tgatgattat
    50281 cttattctaa agctggcttt tttgttggta caaacatgac aactacaaaa aaaccaggcg
    50341 aacgtatgat tgttcgtctg gtttttaatt attaatgact tagcgttttt actagaaata
    50401 gaaaatttgg gcaagtctac tttatatttt tactagaaaa actacttccg ctttttcccg
    50461 ccaaaagcat ctttaaactt atcgaagaaa ccgtcttctt gctgttcttc aactttgatg
    50521 ccgccagctt cagcgaatgc acgtaatgct tctctttgtt gttcatttag atttttcggt
    50581 gtgatcagtt ttactttgac atgttggtcg cctgttgcac caccacgtaa cttcggagcg
    50641 ccttttccac gtaaacggaa gttcgtacct gtctgtgttc cagcagggat cttcaatttc
    50701 acatctccat ggacagttgg tacttggact tcatccccca aagctgcttg tacaaaactt
    50761 aaaggcaatt catagtaaat ttcagaacct tcacgatcaa agatatcact ttcttctaca
    50821 cgaaaaacca catacaaatc accaaatggt ccgccgtttt ctccagcttc tccttgacca
    50881 gctaaacgca tttgttgtcc ttcttctaca ccagctggta cattcacttt aactgaatgt
    50941 gctttcttct catgtcctga gccatgacag gttggacaag gttctttgat ttctttccct
    51001 gtgccatgac aaacatcaca agtttgacgg ctcatcactc gtccaagtgg cgtttggcgt
    51061 tctacattga tcgtacctgc accatgacat ttatgacaag tagttggttg tgtgcctggt
    51121 tttgcaccgt ttccaccaca agtatgacag atttcttccc gattatactt gatttctttt
    51181 tctacaccaa aaatcgcttc ttcaaaagac aagtcgaccg tatattgcaa atcagcacct
    51241 tgacgaggcg catttggatc gactgaccga ccgcctcctc caaagaaaga gtcgaagata
    51301 tcttcaaaac caccgaagcc gccaccagag aagccgccga atccaccaaa tccgccacca
    51361 gctccaccag caccgtaatt tggatctgtg cctgcatgcc cgtattggtc gtatgcagcg
    51421 cgtttttgcg ggtcacttaa aatctcatat gcttctgata cttctttgaa tttttcttcc
    51481 gcatcaggtt ctttattgat gtcaggatgg tattttttcg aaagcttacg ataagctttc
    51541 tttatttcat cttctgatgc tccttttgag agccctaaga cttcataata atcacgtttt
    51601 gttgccatag gctttcctcc tattggttct catcattttc acgtcgcttg ttatcatatc
    51661 atattttgtt tgaaacaaaa accgatactt caaactattt ttcaaaagaa aaagccaaag
    51721 tagcaacttt ggccttctct cgaaattcag tgaactgatt atttatcgtc gccatcgact
    51781 tcttcaaaat cagcatcaac gacatcatct gctccgcctt gagctgcttc tggattttct
    51841 tgagcttgtt gttgtgcagc ttgttcgtat agtttaacag tcaagttttg aacgatttcg
    51901 ttcaatgcat cgcgtttagt tttcatttcc tcgatgttgt ttgcttcaac agcggctttt
    51961 aattcgtcac gagctgtttc tgcttttttc acttcttctt cgtccacttt gccttctaat
    52021 tctttcaatg ttttgtccac agagaatagc aatgcgtcta catcattacg taggtcaact
    52081 tcctctttac gagctttatc tgcttcagcg tttgcttctg catctttcac catacgttcg
    52141 atttcttcgt ctgttagacc tgaagaagat ttgatcgtga ttttttgttc tttttgtgta
    52201 cctagatctt tcgcagatac atttacaatc ccgtttttat caatatcgaa tgttacttcg
    52261 atttgtggaa taccacgagg agctgctgga atatctgtca attggaatct tcctagagtt
    52321 ttgttgtcag cagccattgg gcgttcacct tgtaatacat ggatgtctac tgctggttga
    52381 ttatctgccg cagttgagaa tacttgtgat ttacttgttg gaatcgttgt attgcggtca
    52441 atcaatttag tgaacactcc acccattgtt tcgataccta atgacaatgg tgtaacgtca
    52501 agtaaaacaa catctttcac atcaccagtg atcacgccac cttggatagc agcacccata
    52561 gcaactactt catcagggtt aactgatttg tttggttctt ttcctgtttc tttacgtact
    52621 gcttctacaa ctgcagggat acgagtagat ccaccgacta aaataacttc gtcgatttct
    52681 gattgtgaca gacctgcatc ttttaacgct tgacgtactg gtacttttgt acgttctact
    52741 aagtcagctg ttaattcatc aaattttgca cgtgtcaatg tcatttccaa atgcaatggt
    52801 ccagattctc ctgctgtgat aaatggcaag ctgatttgtg tgcttgatac gccagataaa
    52861 tcttttttcg ctttttcagc agcgtctttc aagcgttgta aagccatttt gtcttgagac
    52921 aagtcaatgc tgttttcttt tttgaattct gctaccatgt agtcgatgat tttgttatca
    52981 aagtcgtccc cacctagatg gttgtcgcca gctgttgaca atacatcaaa gacaccgtct
    53041 cctagttcaa ggatagatac gtcaaaagta ccgccaccaa ggtcgaatac taagattttt
    53101 tcgtcgcgat ctgttttatc taaaccatag gcaagagcag ctgcagttgg ttcattgacg
    53161 atacgttcta cttctagacc agcgatctta ccagcatctt ttgttgcttg acgttgtgca
    53221 tcattaaagt aagctggcac agtgataact gctttttcga ctttttcacc taaatagtct
    53281 tctgcgaatc ctttgatata ttgaaggatc attgctgaga tttcttgtgg tgtatatgat
    53341 ttaccttcta catctacttt gtaacctgct tcaccgatat ggcgtttgat tgatgaaatc
    53401 gtatttgggt ttgtcacagc ttgacgtttt gcaacttccc ctacttggat ttctccattt
    53461 ttgaatgata caacagaagg tgttgtacgg tttccttctg ggttagcgat gatttttgct
    53521 tcgtttcctt ccaatactgc tactgcagag ttagtcgtac ctaagtcaat accgataatt
    53581 ttactcataa ttaacgtctc cttctttaat tgaaagtgta agttttatat ccgcacaatt
    53641 attgtgcaac aatgaccata gttggtctta acacgcgatc atgtaatttg tagccttctt
    53701 gtagtacttc tacaatcgta tcggctggtg tatcttcagt cgctggaaca gtttgtactg
    53761 cttgatgaag attaggatca aaggcttctc ctttagcagg gattttttca attccttctt
    53821 cttccagtgc gttacgcaaa ctttccaaga ccatttccac gccttttttc aaacctgccc
    53881 cttgatcatc tgacacttct gtggctaatg cacgttccag gttgtcaatt gatggaagta
    53941 attttttggc taaatcttgt gaacggtatt tttgtaattg ctcacgctca ttttttcctc
    54001 gatttgccat atttgcgatt tcagcacgag cccgcaagta tttatcttcc atttcttcta
    54061 actcagcttt taatttcgct gtttcgaatt cttccgcttc agcttctgaa atacccgaat
    54121 cttctgcagc ttcgacatcg atttccggtt ctggctgagc atctgtcatt tctttatcta
    54181 gttcttcttg attttctttc acgatacagt cttcctttca ttattcagtc agctgtttga
    54241 ttgctcttac taagaaagag agaacgatca gctgcaaata gcttcgttat tcacattacg
    54301 agtttgacaa gtcaagcgag cggtagtaat cagcaagttt agctgctagt tctcttcgat
    54361 aaacatcgac tagaccgaac atccgagaat aaggcatact agtaggccct aacaaggcta
    54421 ttgtacctct tccatgacca tctatctcgt aacttgcttg aatcatactc atattctgca
    54481 ataaatcatt gcctaattca gcaccaatct tgatatgaat attgctatcc atcggagtga
    54541 tcatttgtgt aagtccgtct gagtctttca taaaagaaaa catcgatttg aactgatcaa
    54601 gatcttgacg gggctcataa tccaagagat tcatttgtcc actgacataa accttttctt
    54661 caaaaacatg accaagaacg gtgtcaaata actgagacat cccttctgtc gtctggaaat
    54721 atttatgaag aatcatcgga atctccgtac gaagtttatg gtacaccgtc atcagaggat
    54781 ctccgactag acggtcgttt acaatctgta ccattttttc tagttcctga cttcccatat
    54841 attcgggaag agtgaacact tggctttcta cattccccat atctgttacg acgatagcaa
    54901 taatctgtcg attattcaag ggtacgatac gaaagccagt cagtcggcgg tcttttattt
    54961 caggtcccaa tgaaaaagtc gtatagcttg tcagctcaga taatatctca gcagattgct
    55021 taataatctc attgatttca tgaaattctc tgcctagaga ctgacgaatc agcagcaaat
    55081 catcttctgc tacgcgagca ggttttaaca aatgatccac gtaataacga tagcctaaag
    55141 cagatggaat ccgcccagaa gaagagtgag tcttcaacag caatccttca tcttctaatg
    55201 ctttcatatc attgcgaatc gttgctgagc tggccttgac tccctcagac attaatgttt
    55261 tggaaccaac gggcacgcca ctactcgtat agtgttggat gatcagacgt aaaatgtcgt
    55321 tttgtcgctt tgttaacatc ttcctcacct ctcattagca ctcatatatt ctaagtgcta
    55381 attcacttat aaatatagca agaagccctt ctcctgtcaa gagaaagact ccatttttta
    55441 gcactctaaa cacaagagtg ctaattctaa ataattgatg taaatccagt tatatcaata
    55501 cttcaatacc tcattgtcaa actttctaaa ctttttcttt tcaagtttcg ataaaaaaca
    55561 aatattcttc aaactatttt tcaaataaaa agcgctcaaa cacattgttc cccaaaaaag
    55621 tccctcttgg tgtcaaataa acacggtctt cttcattaac aagtaaccct tcttgctcta
    55681 attcccttag cacatcaccg tacaccgatt caattgtttt ttgatagcga tcatagaaat
    55741 gttttttagt aatccctgtt ttttttctta gtcctaagaa catttcttct tctatttgat
    55801 tttttcgtgt caatttttct tcttccaata ccgggcgttc atttgctcgt aatgggcgta
    55861 aatagtgttg gattggtcct ttgttgcggt aacgaatatc tccaagataa ccgctagcac
    55921 cagctccaaa cccaaaatag tggtcattgt tccagtacat gaggttatgt tgagattcat
    55981 gtccggagag cccaaagtta cttatttcat attggtgacg tccatgcttt tccattgctt
    56041 caatcgtctc ttcaaacatt ctggtttccg tttcctgatc aggcagttcg agtcttcctt
    56101 gtcgtaccca gttcatgaac attgttttgt tttcgaggat caatgagtac aaagaatagt
    56161 gaggcagatc caaagccaaa gctttttcca aagtatcccg atatccttct agtgtttgtc
    56221 ccggtaatgc ataaatcaaa tcgatgctta cattggtaaa attttctgct tctaaaaatt
    56281 gcattgtttg atacacatct tctgcagtat gtttacgtcc aatttttttc aataagcgat
    56341 tgtcaaatgt ctgcactccc atggataaac gattgacgcc atagtttttc atcacttgaa
    56401 gtttttcacg ggtcaaatca ccaggattcg cttctacagt aaactcatta ccattttgaa
    56461 aaggcaggat ctctctagca cctgataata gtcgatcaag ttgtttagca gataaagaag
    56521 ttggtgttcc gcctcctaca taaagtgttt ccaaattcgc tgtaggatga ctttccagca
    56581 ttaatctcat ttcattcagc agcatttcca catactcatc tactggctgc ccttctaaaa
    56641 aaactttatt aaagtcacag taatagcaga tatgctcgca aaatgggata tgaatgtacg
    56701 cagaaacctt gttttctgcg ggatttattg tttctatcat tgaaattctt tctccttacg
    56761 tcgaaaagtc gtgcctgttt tcgaccttct taaaatcgcg ttcaagcacc aactcattga
    56821 aataagccgc atatttagaa tcaaggaaac ctagctctaa aaatgcttgc ttattgtcat
    56881 cggagctaca cggtgctttt acagcctctc gataaacggt aatcgcaaag ctgaccttcg
    56941 gcattaagtc aaaatctgca aaaatatgaa aagctatttt cacagattcc tccttaagtg
    57001 ctcagaccac aacgctttgc ttacaacctc tcatatacgc atactaaacg tatttggcga
    57061 acaaatccac taaaaaatcc aagaatcgac ttcttggatg atttttttac actcatgatt
    57121 cttcctaaac aattttgtcc ttattttatt cataaaaaaa tctagtcaaa tctctcactt
    57181 caagagtttt aactagattc caattattat ttttttgtaa aaataaacag tctcttaatc
    57241 atagacattt cttcggtgcc ctacttgaat agcgataaca acgaattgat tatcttctat
    57301 ctctaccaat acacgtattt gccaatcaga gctgcttctg aatttcgggc gtgaatttta
    57361 aacggcaggt cattccagcc caccaaattc attaaggatt cttccatgaa tactgttgtt
    57421 ttcccacttc ttatccaatg tttatgggca atttcagccg tttgacgatt gtattcatct
    57481 tctagttgtt ccatcgaata gttttttaga agttcagata aagtaatccc ataaaattca
    57541 gccatataag aaagccattc ttttttggcg tcacttacac gaaccgtaat catagcccta
    57601 taattgcctc tcttaaagaa tctcatgcat ttcctgatgt gattttagaa aaatttactt
    57661 gctaaaaaac tgtgaaaatg taggagctag catccttgta gtaaatgatc gagctcttta
    57721 ttatacatta aaaaagttca gaaattttga tgaagtagcc aactactttt tcatgagtaa
    57781 agcaacacta gaaaaagaat cctccagtac ttagaattta ctggaggatg tacttttgaa
    57841 gaagcctata ggttatttaa aaaatattac tacaggaagg aacgagaatt acttcgttac
    57901 ttataataga acttagtctt atatagaagg catagctcac attagcttat ttgtcctata
    57961 aaaataaatt acggtatttt agaaaagcta ttttctcttt tttcaacgat cttcagcaat
    58021 ttttctttat cgtcaggtaa ctcaagttca tgaattctag catgacaatt gggacataac
    58081 gcgatacagt tttcaatcac atcttttcca ccttgcgaca aatattctat atgatgacaa
    58141 tgtaaataag gtacaccgtc ttttataaat ggcccttttt cttggcaaag ttgacaaata
    58201 ccttttgcaa gtcgtttaac atattcagat acgaagggat tacgtttata tatttttgtt
    58261 tttgagaagc aaaactcctc ttcgttaaca tattttgcag ccattttttt attataatta
    58321 ctaacttttc ttgcttcttc ctctaattta gcattagata aattacgaat atgtctagtt
    58381 tgattttctt cctggttata tatctttatc ttcaatcaat tgctcttgat ttattaaagc
    58441 taatggaaat ttatatactt ttctgctgtt gcccgaacta tctttttcat taacagtata
    58501 tatttgtcct gctaaatata cttctcccag atatgtgtgt tcttttggtt taaatgtttc
    58561 aaataaatga atctttacaa aattactttt tgaattggct aaaactttat tttgattctt
    58621 atccaatact tgatcccctt ttagccccat accggtatat tgtaaaatac catttaacca
    58681 cttatcttta taaacattac ttcctgtttg atcactaaac agtacaagag tattagtttg
    58741 atgagatctt ctcattccac cctgtttgga acatttaaac ttctctgaaa tttctttgtt
    58801 agtatatact ctaccaactt ctaactcacc taatatgttt ttagaccaat tttctcgcca
    58861 tgtaataatc ttatcaagat taaacatttc tccctctttt ggcatgccat ttctaattaa
    58921 attagctaat acaccagtag aaatatctaa tttttttagt aattctttct tagtaatcaa
    58981 tgttggattc ataacaatgc agctcctaat gatatcaata taattgcatt agataacttg
    59041 aaaaatcgaa aaaaactcat t
//
LOCUS       DAMYXM010000013        53362 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.013, whole genome shotgun sequence.
ACCESSION   DAMYXM010000013 DAMYXM010000000
VERSION     DAMYXM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.013"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            8..310
                     /locus_tag="PNS21_000896"
                     /pseudo
     CDS             8..310
                     /locus_tag="PNS21_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
     gene            558..800
                     /locus_tag="PNS21_000897"
     CDS             558..800
                     /locus_tag="PNS21_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158623.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKDLLIKVK"
     gene            complement(905..2113)
                     /locus_tag="PNS21_000898"
     CDS             complement(905..2113)
                     /locus_tag="PNS21_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEnfa110 family transposase"
                     /protein_id="HDI5158624.1"
                     /translation="MNCVGIDVSKGKSMIAVMRPFGEVVVSPFEVRHTANELSELAGL
                     LKSLDGETRVVMESTGNYHAPVAWLLHDAGLYVSVVNAMLVHDYGNNSLRRAKTDKKD
                     AVKLANYGLDHWLTLPRYIPEEDTRLMLKICYRQYQQYSKVQTMLKNNLISLLDTTFP
                     DANRLFTSPPRADGSEKWVDFVATFWHCECVCGRSEKAFTTQYQKWCRKHGYNFSEDK
                     ALDIYASACRHFGVIPKTDTAKLLVEQAISQLQTTSSALAALKQEMQSLAASLPEYPV
                     VMGMFGVGPTLGPQLLAEIGDVRRFHSKKALVAFAGIDAPPYQSGQIDVRSRSISKRG
                     SASLRRTLFLVMSVILQCAPIDEPVYQFMDKKRSEGKPYRVYMMASANKFLRIYYASV
                     KAYLESLEHD"
     gene            2403..3368
                     /locus_tag="PNS21_000899"
     CDS             2403..3368
                     /locus_tag="PNS21_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5158625.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            3390..3602
                     /locus_tag="PNS21_000900"
     CDS             3390..3602
                     /locus_tag="PNS21_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDI5158626.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            3677..4153
                     /locus_tag="PNS21_000901"
     CDS             3677..4153
                     /locus_tag="PNS21_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HDI5158627.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            4150..4650
                     /locus_tag="PNS21_000902"
     CDS             4150..4650
                     /locus_tag="PNS21_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HDI5158628.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPS
                     APVRAV"
     gene            5210..7027
                     /locus_tag="PNS21_000903"
     CDS             5210..7027
                     /locus_tag="PNS21_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDI5158629.1"
                     /translation="MRNPIHVLKSLEEKASVSNYKYERLYRNLYNPEFYLLAYANIAK
                     SQGSMTQGVDGQTLDNMSLPRINRIIESIRNRTYQPKPAKRKYIPKKNGKLRPLGITS
                     TDDKLVQEVVRMILEAIYEPTFSNNSHGFRPKRSCHTALTQVKKNFTGVTWIVEGDIK
                     ACFDNFDHHVLVELLRKRISDEAFIGLIWKFLKAGYMEQWQYNCTYSGVPQGSGISPI
                     CANIYLSELDNYMQEYKEKYDCEPERRRTTREYERASRRYRKARKALMGAEKSTPELV
                     KEFKDSRRKKMNQHYYNPFEEGFKKIQYNRYADDFVIGVIGSKKDAEKIKEDVKIFLQ
                     EKLHLEMSEEKTKVTHSSKPVRYLGYDFKVIHSKNMKRCKNGDMKRVWYGKVFLYMPK
                     EKWIKKAMERGAIQVKRNNDTGKEMWRPMPRKDLMNRSDAEIVSTFNSEIRGLYNFYR
                     IAENVGALHKYYYMVRYSMLKTLAGKHRTNVSVIKKRHMVNGVLRIPYDTTKGRKYCE
                     FYHDGFRKHSDGYDNVADVMPSYRKYDSRHTIVNRIKAGVCEICGEHADYLCMHHVRT
                     LKSLKGRDIFEQKMLKMRRKSLALCPDCFELLHETKESR"
     gene            7133..8461
                     /locus_tag="PNS21_000904"
                     /pseudo
     CDS             7133..8461
                     /locus_tag="PNS21_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
     gene            8538..8699
                     /locus_tag="PNS21_000905"
     CDS             8538..8699
                     /locus_tag="PNS21_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HDI5158630.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKHLMSSM"
     gene            8740..9120
                     /locus_tag="PNS21_000906"
     CDS             8740..9120
                     /locus_tag="PNS21_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HDI5158631.1"
                     /translation="MTISCKAKGDTMKIEYTKVGDYYLPNLYYPEETRPIGLWGMLRK
                     EYLKEHKSGTYTYLLLTARLDSYLADLNEQAQERFELIETQMRSAEGVSEELKRQNPM
                     EWLCHCNNIRNRAAEIIKQELIYV"
     gene            9135..9416
                     /locus_tag="PNS21_000907"
     CDS             9135..9416
                     /locus_tag="PNS21_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158632.1"
                     /translation="MRRELIESYYEEYQRELERFLESDPEMFTLEAEIHHYIEILEPV
                     LRDGGQEMWQTLDRIISAKNAMEGIVAKEMYIRGFLDYERLVCEKDSKI"
     gene            9675..10457
                     /locus_tag="PNS21_000908"
     CDS             9675..10457
                     /locus_tag="PNS21_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDI5158633.1"
                     /translation="MKVKNADEFYKILERKAIYPVFQPIVNLQTGDVAGYEALSRIDR
                     HDTTLMISDLFVIAEQVGCVWKLEKLCRNKALKAAANKPEHAKLFLNVDGNIIQDKSF
                     IQGFTNRKAAKAGVPSCDIVFEITERSDIENYQILQQIMKHYADQGYEIALDDVGAGY
                     SGLNRVVNTSPNYLKVDIELVRDIQKHKKKEIMMEFLLHYCNETGAILIAEGIETEAE
                     LECLCRLGVHYGQGYFLGRPDRTFKNVRNEAMLVLRRLNNKS"
     gene            10630..12348
                     /locus_tag="PNS21_000909"
     CDS             10630..12348
                     /locus_tag="PNS21_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDI5158634.1"
                     /translation="MNENVKVVFGLIGGLALFLYGMNSMSDALQKAAGERMKKILGFL
                     TRNPIMGALAGALVTAVLQSSSATTVMVIGFVSAGLMSLPQAISVIFGANIGTTMTAQ
                     LMAFKISNYIYPIIFVGFILNFVSKKEKVKNIGMVIFSFGLLFEGIEIMGEVMKPLAG
                     SPVFVDLMGKVSSIPVLGVVLGAVMTLVVQSSSATIAVLQNFASQAGPDGVSSVIGLT
                     GAIPILLGDNIGTTITALLASIGQSKNAKRTAIAHSIFNISGSCVFIFLVPWFAKFVQ
                     FISPKGNEIDVISRQIANAHTTFNIVCTLVWLPLIPLMVKIVTTIIRGNDKTEKAAFE
                     PKYLDMKVIEQPAAAMVLVSKELNRLGELAESLLSDLKTAIVADGDSKTHGSFIENLE
                     IVHQLQDSVTEYITRLFASGNLTEQQSEQTAGLLYVNNSIQRIADRCEDIDQICEKVN
                     NNGKRLTSEASSEMEDCIDIIQQLLKKAMEAIRKGDAVVAETVFKNKKKMHKAEKKYS
                     KAHLNRVKNEVCDASMTRYFSGIMYNFDRMADNCVSIAEEACDNVAFINLDEETGKSM
                     MERGAV"
     gene            12350..13204
                     /locus_tag="PNS21_000910"
     CDS             12350..13204
                     /locus_tag="PNS21_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDI5158635.1"
                     /translation="MVKKKNIRKSALLLIMIMLATGITACGTEEEPLPDLSAMGAVTA
                     VSREEGSGTRAEFENLLKLPESDTGIVVDSTEKVLKKVEEDKNAVGYVAYSSATDTNG
                     KILQINGVLPSEKTIDNNSYPLCRDYYLAYNGELTDVEQDFLTYVKSKGQDIVKQYCI
                     QADSTTTFLSDKSEGKILIEGSTSMEPMVKALADDYQKQNPNAEIEVKATDSSRGITA
                     VISGECDFAMSSRELKDYEAELLETKVIGKDAIAIVINEENPLENLTIKQLTGLYNGT
                     YKNWDDLS"
     gene            13880..14200
                     /locus_tag="PNS21_000911"
     CDS             13880..14200
                     /locus_tag="PNS21_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158636.1"
                     /translation="MPGKEQFMTELKMKEMQLINWFGCPNLIYTRERIHLVAMLTTDE
                     EMKKNLYRVCRFLAREEIAYRYPIMYEMIRKIFSPDDTNPPEACAMFMPDDETDDEEY
                     AMAA"
     gene            14224..14634
                     /locus_tag="PNS21_000912"
     CDS             14224..14634
                     /locus_tag="PNS21_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI5158637.1"
                     /translation="MNKVVLMGRLTKNPEIKYAGKDNDMAVARYTLAVNRRYKRDGEQ
                     EADFISCVTFGKSAEFAQKYLHKGMRIVIGGRISTGNYKDKDGKTIYTTDVIVEEHEF
                     AQNKDNGVGADSSETPKTDKDGFMEAPEGEVPFD"
     gene            14781..14987
                     /locus_tag="PNS21_000913"
     CDS             14781..14987
                     /locus_tag="PNS21_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI5158638.1"
                     /translation="MGVSYNRLWKMLIDKNMKRIEMQYLTGISGNILARMGKNQYVSM
                     ETIEKICKKLDCTVDEMLEFTDDE"
     gene            15334..15855
                     /locus_tag="PNS21_000914"
     CDS             15334..15855
                     /locus_tag="PNS21_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5158639.1"
                     /translation="MIRKAILKDVNSIEDTYNEHFQYELNHTAFTVFKKGVYPTKDDA
                     ERAIYAGALFVYEENGTIVGSIIIDKVQPIEYATIPWKEKLSEDEVMVIHLLMVRPSM
                     SGKGIASSLIKFATELAQKNSCRALRLDTGSQNIPALSLYQKNGFEIIASASKKVGDV
                     IAHKNHLFLEKIL"
     gene            15913..16338
                     /locus_tag="PNS21_000915"
     CDS             15913..16338
                     /locus_tag="PNS21_000915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI5158640.1"
                     /translation="MIRELRKVDINKVAEIWLDTNIKTHYFISAQYWKSNFELVKELL
                     LQATVYVYEDKQEIQGFIGLSNEYIEGIFVSAEMQSQGIGKILLNYVKGKRNKLILNV
                     YQKNTRAISFYQREGFEIQYSGLDEATGEKDYVMAWQQK"
     gene            16517..16747
                     /locus_tag="PNS21_000916"
     CDS             16517..16747
                     /locus_tag="PNS21_000916"
                     /inference="COORDINATES: protein motif:HMM:NF025559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HDI5158641.1"
                     /translation="MQDDDSGFTYEISRCLYFDTCKEHGYAEFCKVFCNHDWYAYGVL
                     KHHSRFIRKSTIAEDGTVCHDTIEKVTEKHFL"
     gene            16781..16963
                     /locus_tag="PNS21_000917"
     CDS             16781..16963
                     /locus_tag="PNS21_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylan 1,4-beta-xylosidase"
                     /protein_id="HDI5158642.1"
                     /translation="MAKEERFEKIYSQGALTTTEIWVDKETGVNYLYHACGYSGGLTP
                     LLDRDGKPVISQAINR"
     gene            17076..18587
                     /locus_tag="PNS21_000918"
     CDS             17076..18587
                     /locus_tag="PNS21_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158643.1"
                     /translation="MRRSGWTKWLIVIPAMLGIVLVTYVRYTTDIWGFGAFICQLIAI
                     LELAYGLRIAMLAQNRKKSYRLTPEERHEYARYLYEKQYHRYPAVANQMLLVMARMSV
                     LLNNYERAAQELADIRIDKFNPAQLKLYYYLKVVTAMAAGDATGIQESQMCYAGIPDT
                     KCTYPSKAELAMWIEQRAAAQMAEALKKAVPDKKEHPIRISIITLVLAYSTFFYGLWY
                     GIKRDMGYEVRYRFAEISITVITISLAVLLIWGIVSLYRHNCQALSIQFGKNKVALII
                     VYGAAAIFALLIIGMSGLSVVFGMDGTETVTGCDSKYTYITVQEDYGGERRYRTNNPF
                     VMQSMDGLWWSSDTIDEEDNQEEDTTELPDLDTENSTDSEDIQSGNTIEDYETEGQHD
                     GLTIQNAMLAVYNYLQEQNTLQNMAFSYTANAKGEVYAVVSETQETKNGNTVNVRYCL
                     YDNGSKTDADGNSCEELVLEKVYPDGNYETELVDFYLVNADTMQVTDEQKNTW"
     gene            18910..19134
                     /locus_tag="PNS21_000919"
     CDS             18910..19134
                     /locus_tag="PNS21_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HDI5158644.1"
                     /translation="MSEKTEITFMQTRLIRLASEEWHLPVEQIIHLFKEADVLGYIEK
                     CYGIFHCEGDEAVLEDITEFLQRKGIEISA"
     gene            19127..19693
                     /locus_tag="PNS21_000920"
     CDS             19127..19693
                     /locus_tag="PNS21_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HDI5158645.1"
                     /translation="MLELKDGFVLYHGSYCEVKEPDLAKCAKRKDFGQGFYLTTSKEQ
                     AESFLRTSIAKAIATGTIEEGQKFGYISTFEFKLSENLETHIFENADVDWLHCIAAHR
                     KKKMFIEVEREMAKYDIIAGKIADDATNATLTAYLAGAFGTAGDKVADDFCIRQLLPN
                     KLKDQYCFKTEAAIECLKFVKGEKIWLK"
     gene            19681..19911
                     /locus_tag="PNS21_000921"
     CDS             19681..19911
                     /locus_tag="PNS21_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158646.1"
                     /translation="MAEVTIPKEKMNYTIDLLITMVTDEIAEETGKDRNEVLTDFLCS
                     KTGKALYDEKTKLWCTGPAYIAELYMEELKKS"
     gene            20112..20477
                     /locus_tag="PNS21_000922"
     CDS             20112..20477
                     /locus_tag="PNS21_000922"
                     /inference="COORDINATES: protein motif:HMM:NF024360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HDI5158647.1"
                     /translation="MSRAKNPKYESMSLEELKANMEKSRQELEAAIHHKNILEQRKKL
                     VERRERSHRLIVKGAEFEKVFPLSRDLEQEDVQDMMNQLQNSSYNKSIVRQAHIAALH
                     KDQQQIAEAVKKTEKGDDS"
     gene            20477..22204
                     /locus_tag="PNS21_000923"
     CDS             20477..22204
                     /locus_tag="PNS21_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HDI5158648.1"
                     /translation="MALYHFSVRNVSRGKGQMVVASAAYISGQRIFDSYYNKIHDYTS
                     KSGVIFTEILTPEYVPERLTDRETLWNEVEHVERNNKAQLAYSFDIALQNELTLEENI
                     RLAKEFCQEQFVARGMIVDLAVHEGKAENEEEPDNPHFHVLVPIRPFTEEGIWGNKQR
                     REYILDEDGNRVKDAKGKDMFNAVSTTGWNDPELLKEWRRAWTEKVNGKFRECHMASR
                     IDHRSYKEQGIDLIPTIHEGYEVRAMEKKGIKTVIGELNRAIRQFNQMFISLKESIQW
                     MKTAYEEMKAELDRRQNPTLLESLQDYYDKKTQGRPPLPNFYAEMKQRGKNLSNLQEF
                     AKSISYLQTHQIETMDDLQERIEELNGVVSVSKKEISEKRKQLKELENLEKMAEVIKT
                     NQPLIDEYNHFYFQKRRERYYQQHKKEINYYRKCERELKQHLDKNGKVPTARWKREKE
                     ELQAGIEELKADNQPYQEELTFVKKVQSCADIARRDREMAEADTSGRSEEKREKQVKF
                     PTFYTVQAKEILEENGKAEQQPLNQSEQNPEKKTSLLRKLDEKKKECAERDAKQQAVK
                     KKRNYDMSL"
     gene            22234..24150
                     /locus_tag="PNS21_000924"
     CDS             22234..24150
                     /locus_tag="PNS21_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI5158649.1"
                     /translation="MSKMQYTEEQIIRANQTDLVSFLSAQGEQLGKSGKEYRWKKHDS
                     VTVSGNRWYRHSQGRGGYPVDFVMEFYNATFPEAVKMLTGEEGEGRNSTCPAPSPDFR
                     LPEKEENNDRIIRYLTENRGIEKNMVEEWIGSGDIYEEKKHHNVVFVGRDADGIPRYA
                     HCRGTGETKYRGDVAESDKSYGFCHRGTDNQLFVFEAAIDLLSFIQLFPKDWKKRSYL
                     SLGGISSAALMAFLSERPQITSVFLCLDNDHAGNEASEKLAIEIPDGYSVIRLKPSRK
                     DWNEILCDKNADRKKSIIETVTMKVPEKEELVPMLCYEDIEQTSVEWLWFPYLPFGKL
                     TIIQGNPGEGKTYFAMMLTAACTNRKTFPNMEEIEPFNVIYQTAEDGMGDTIKPRLVE
                     AGADLSRVMVIDDTEEALTLSDDRIEKAIRQNQVRLLIIDPVQAFIGADVDMNRANEV
                     RPVFRKLGMIAEKTGCAIVLIGHLNKSSGTQSTYRGLGSIDIMAAVRSLLFIGKVKKD
                     PTTRVLIHEKSSLAPPGETMAFKLGDEEGFRWVGAYEISADDLLDGKEGKPTETKLQR
                     GTKLIYELLADGNAVTIRELDEKAKAQGISQRTMREARSRMKEELDYRMNEKQENTIR
                     LKKQGRMGDGRILE"
     gene            24835..25017
                     /locus_tag="PNS21_000925"
     CDS             24835..25017
                     /locus_tag="PNS21_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158650.1"
                     /translation="MAVKKKIINIRVYFDGDQDAKEVFAEVIAQKIRDTKTKDKLAIM
                     KDKQYNDYEFSNLKSA"
     gene            25044..26741
                     /locus_tag="PNS21_000926"
     CDS             25044..26741
                     /locus_tag="PNS21_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI5158651.1"
                     /translation="MNNRYMSNNIERNATWRVGVYCRLSKDDELTGESASISNQRDIL
                     VNYCQSQGWQVVDVFQDDGYTGLNTDRPDLQRLLKACEKGLVNLVITKDQSRLGRNHV
                     QTGFLMEEFFPKHGVRYIALYDNVDTFAGDNEIAPFKNVLNEMYSRDISKKVHASYHL
                     QATKGKFTGVVPPLGYQKDPEEKGHLLIDDETAPIVRKIFQWAVDGHGINFISRELER
                     QQIPCPSWWHRKRGLRTYYTKWEKKDPENGKYVWDESYLKSLLMNPVYCGDMASQKRY
                     YRFKIGNQGDKAPEDWITVRDTHEAIIPQDVFDLVQAKMKGRKRQTEQGEYSMFAGLL
                     RCAECGGALTLKKTHTKDQHEVYTCSTYIHKGKAHCTQHRVDADDLYDAVLTRIQECA
                     KAVTGDGTELENRVKELCEEDTQGHRESLEKLVSKQKDRLETLDRLIAKLYDDLINDK
                     ITESVFDKMLEKTQKEQTDIKKELFQNESVLNTEEKLDAQSQQWIDDISEYADIKELD
                     ANLLNRLISKIVISEPQEKDGTENYCRTPETVTMEIHFNLKPIPELGTIERGSGSHK"
     gene            26766..26897
                     /locus_tag="PNS21_000927"
     CDS             26766..26897
                     /locus_tag="PNS21_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158652.1"
                     /translation="MLIFSRLISSKYFLIKDIKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            26989..27852
                     /locus_tag="PNS21_000928"
     CDS             26989..27852
                     /locus_tag="PNS21_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158653.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            27889..28425
                     /locus_tag="PNS21_000929"
     CDS             27889..28425
                     /locus_tag="PNS21_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HDI5158654.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            28439..28861
                     /locus_tag="PNS21_000930"
     CDS             28439..28861
                     /locus_tag="PNS21_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HDI5158655.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            28791..31190
                     /locus_tag="PNS21_000931"
     CDS             28791..31190
                     /locus_tag="PNS21_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HDI5158656.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            31222..33213
                     /locus_tag="PNS21_000932"
     CDS             31222..33213
                     /locus_tag="PNS21_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDI5158657.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            33236..33487
                     /locus_tag="PNS21_000933"
     CDS             33236..33487
                     /locus_tag="PNS21_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HDI5158658.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            33477..34706
                     /locus_tag="PNS21_000934"
     CDS             33477..34706
                     /locus_tag="PNS21_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HDI5158659.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            34703..36784
                     /locus_tag="PNS21_000935"
     CDS             34703..36784
                     /locus_tag="PNS21_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HDI5158660.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            36933..40853
                     /locus_tag="PNS21_000936"
     CDS             36933..40853
                     /locus_tag="PNS21_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HDI5158661.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            40854..41798
                     /locus_tag="PNS21_000937"
     CDS             40854..41798
                     /locus_tag="PNS21_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158662.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            41802..42005
                     /locus_tag="PNS21_000938"
     CDS             41802..42005
                     /locus_tag="PNS21_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HDI5158663.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(42305..42541)
                     /locus_tag="PNS21_000939"
     CDS             complement(42305..42541)
                     /locus_tag="PNS21_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5158664.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(42724..43110)
                     /locus_tag="PNS21_000940"
     CDS             complement(42724..43110)
                     /locus_tag="PNS21_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HDI5158665.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(43092..44420)
                     /locus_tag="PNS21_000941"
     CDS             complement(43092..44420)
                     /locus_tag="PNS21_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDI5158666.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(44381..44710)
                     /gene="mobC"
                     /locus_tag="PNS21_000942"
     CDS             complement(44381..44710)
                     /gene="mobC"
                     /locus_tag="PNS21_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HDI5158667.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(44968..45339)
                     /locus_tag="PNS21_000943"
     CDS             complement(44968..45339)
                     /locus_tag="PNS21_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI5158668.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            45484..45669
                     /locus_tag="PNS21_000944"
     CDS             45484..45669
                     /locus_tag="PNS21_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI5158669.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            45827..45994
                     /locus_tag="PNS21_000945"
     CDS             45827..45994
                     /locus_tag="PNS21_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158670.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            46612..47271
                     /gene="vanR"
                     /locus_tag="PNS21_000946"
     CDS             46612..47271
                     /gene="vanR"
                     /locus_tag="PNS21_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HDI5158671.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            47274..48617
                     /gene="vanS"
                     /locus_tag="PNS21_000947"
     CDS             47274..48617
                     /gene="vanS"
                     /locus_tag="PNS21_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDI5158672.1"
                     /translation="MERKGIFIKVFSYTIIVLLLVVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQNKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            48788..49594
                     /locus_tag="PNS21_000948"
     CDS             48788..49594
                     /locus_tag="PNS21_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HDI5158673.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            49612..50439
                     /gene="vanW"
                     /locus_tag="PNS21_000949"
     CDS             49612..50439
                     /gene="vanW"
                     /locus_tag="PNS21_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HDI5158674.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            50436..51407
                     /locus_tag="PNS21_000950"
     CDS             50436..51407
                     /locus_tag="PNS21_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HDI5158675.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            51400..52428
                     /gene="vanB"
                     /locus_tag="PNS21_000951"
     CDS             51400..52428
                     /gene="vanB"
                     /locus_tag="PNS21_000951"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HDI5158676.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            52434..53042
                     /locus_tag="PNS21_000952"
     CDS             52434..53042
                     /locus_tag="PNS21_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HDI5158677.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(53108..53281)
                     /locus_tag="PNS21_000953"
     CDS             complement(53108..53281)
                     /locus_tag="PNS21_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158678.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT        15296 a        11862 c        13963 g        12241 t
ORIGIN      
        1 aaagaagttg aaaacgatac ctgattacaa aaaaataact tttataagtt ctataacgtt
       61 gattatattt ttaattctta ttcttctcgt cgtcttggtt ccccgaattt ttggtttgac
      121 tccttatatt attgaggaca aagaaatgag tccatattat gcacaaggaa atctgatata
      181 tgtacgaccg aaaaatacaa atgagatttt agtcggagat attattacat attatgaaaa
      241 ttcaggaaaa aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc acgaagattt
      301 ttcctcaaaa ttttaggtta tcgctgaaag ccccggaaat acgggcattt cgcaggatat
      361 gagaactcaa tttactacca atttactact ttcggattga gccgcatata tgctgaacca
      421 tactgctaat acccgccacc ccaaatacgg cacatcataa attgaatacg acaccgtaaa
      481 cgcggcgttc ctctttcccg tcatgggaga acaggacgcc gcttttttat gcccaaatag
      541 agaaaggagg cgacgccatg ctatttactt ccggccctga ccgggcgttt gaacggctga
      601 tgcaggaaaa gcccggtttc gatcactacg acggcggcgg ggcggacgcg ccggaggatt
      661 gcggcacctg ccgattttac cgcccggatt ggaaatatca attctgccag tttgcagagt
      721 gtccctatca gccgggcaag ctgaccgact atgcagcggt caaattcaca gtgaaagacc
      781 tgctaataaa agtcaagtag aaatttcaga tttttaggga ggcgaaaaaa tgcaatacaa
      841 aatcaagccg ctgagcatct caaaattaaa acggcggcca gccagatggt atagagtgta
      901 aaaatcagtc gtgctccagg gattccaagt aggctttcac agaagcgtag tagatgcgca
      961 ggaacttgtt ggcagaggcc atcatgtaga cacgatatgg cttgccttca gagcgtttct
     1021 tgtccatgaa ctggtacacc ggctcgtcta ttggagcaca ttgcaggatg acgctcatca
     1081 ccagaaagag cgtcctgcgc agtgaggcag atcctcgctt ggaaatgcta cggctacgga
     1141 catctatttg accggattgg tagggcgggg cgtcaatacc cgcaaaggca accaacgctt
     1201 ttttggaatg aaaacggcgt acatcgccaa tttcagccag gagttgaggg ccgagggttg
     1261 ggccaacacc aaacatcccc atcactacag gatactccgg cagagaagct gccagggatt
     1321 gcatctcctg tttgagagca gctaacgcgg aagaagttgt ctggagttga gaaatggcct
     1381 gttctaccaa aagttttgcc gtatccgttt tcggtatgac accgaagtgt ctgcaagcag
     1441 aggcataaat atccagcgcc ttatcctcgc tgaaattgta gccgtgcttt ctgcaccact
     1501 tctggtattg ggtagtaaag gccttctcag accggccaca aacgcactcg caatgccaaa
     1561 aagtagcgac aaagtccacc cacttctcgc tgccatcggc gcggggcgga ctggtaaaca
     1621 agcgatttgc gtctgggaag gtggtgtcca gcagggagat caggttattc ttcagcatgg
     1681 tctgaacttt ggaatactgt tggtactgcc ggtagcagat tttcagcatg agtcgggtat
     1741 cctcctccgg gatatatctc ggcaatgtga gccagtggtc aagaccgtag ttggccagct
     1801 ttacggcatc ctttttgtcg gttttggccc gtcttaaact gttgtttccg tagtcatgca
     1861 ccagcattgc attgactacc gagacataaa ggcccgcgtc gtggagcagc caggccaccg
     1921 gagcatggta attacccgtg gattccatca ccacacgggt ctcgccgtcc aggcttttga
     1981 gcagtcctgc cagctcgctc agttcattgg cggtgtgacg tacttcaaag ggtgaaacca
     2041 ctacctctcc gaagggccgc atgactgcaa tcatgctctt acccttggaa acatcgatgc
     2101 caacgcagtt cattcacatt cctccaatac aaagaatctg caaccggaat ccatcttttc
     2161 tcgctgccga ttcaatctat tgtgtgacac gaactctccg acatccggtg gttcaacctg
     2221 cttaaatcga acgctgcaac gagaggatgg ctaacagtct ttcacacgga catacaagcc
     2281 caaggagtga ttggtcagcc agttgctcct ctcattgtag cttaggcacg agatggaggg
     2341 aaagccggac tggctgtccg gctttcctgt ccaaatttat tgtaatagga gcgaacaacg
     2401 ctatggcagt tttccgtgtg gaaaagaaca aaggctatac ggtgatgtca aatcaccacc
     2461 tgcggaataa gaacctgacc ttaaaggcca agggcctgct gtcgcagatg ttgtccttgc
     2521 cggaggattg ggactatacc ctaaaaggac tgtcctatat caaccgggaa agcatcgacg
     2581 cgatcaggac agcggtatgg gagcttgaaa aagctggata tatccggcgt gagcagggcc
     2641 gtgacgcaaa aggcaaaatg gccgatatgg tctataccat ctacgaacag ccggtattgg
     2701 aaaacccggt attggaaaat ccaacatcgg ataacccggt gttggaaaat ccgacatcgg
     2761 ataatccggc gtcggaaaat ccaacgcaat taaataaaga agtacaaaga actgacttat
     2821 caaaaaaaga aaaaataatt acagatggac aaagtaccca ttccattcct atcccatccc
     2881 cgacccctgc ccctatggag ggcgaggcag cggctacgcc gccggaacgg aaaaggacag
     2941 gaaaggatga ggctgtacgg atttatcggg aaatcatttt ggagaacatc gagtatgcct
     3001 atctcattca ggacaggagc attgaccgtg aacagcttga cgaaatcgta gacctgatgc
     3061 tggaaaccgt ctgcaccgcc cgaaagacaa tccgtgtcgc cggggacgac taccctgccg
     3121 agctggtgaa atccaagtac atgaagctga ccggcgaaca tatccgcttt gtccttgact
     3181 gtctgcggga gaacacaacg aaagtccgca atatcaaaca atacctgcgg gctatgctgt
     3241 tcaacgcccc cagcaccatt tccaactact acacggccct tgtcgcgcac gacatggcac
     3301 agcccgattg gggaaagcct aaatccggcc tgccggacta ttcctgcggc gagggcgaaa
     3361 gcctgtaacg agaaaaggag gacgacccta tgaagcaggg cgcattgatt tttgacgagc
     3421 gaacagaccg ttacgacatc cgctttgatc tggccgacta ttacggcggc ctgcattgcg
     3481 gggagtgttt cgacgtgatg atcggcggca aatggaggcc cacccgcatt gaaatggccg
     3541 aaaactggta tcttgtcggc atccgggccg acgacctttc cggcctgcgg gtgcggattt
     3601 gacctatatc tacctgcggc gtctggtggt tccgccttag acgccgcttt tgactttctg
     3661 acaaggaggg atgcgattgc aggaagaagt tactcaaaag accattgccc tgtccatgaa
     3721 aacgggcaag ctcacggctc aagtgctgca agcggcgctg aaaaagtacc tgcaacaccg
     3781 ggccaagggc aaaaacacgc tgcaccacgg ccagcagagc ctaaagcagc ttaaaaagca
     3841 tggggctgat ctctccaaca ttgagatcac cgaggccaac attggagcgt tcaagccatg
     3901 cgccaagaaa tacggcgtgg attttacctt acgaaaagat aagaccaccc agccgcccca
     3961 ctacgttgtg attttcaagg ccaaggatgc agataacctt gatctggcgt ttcgggagtt
     4021 taccgccaag acgctttcca aggagcagcg gccctctatc cgcaaggttt tgtccgctgt
     4081 gaagcaaaag gcggcggccc agaccaagca gcgggccaag gagaaaatca aggaaagggg
     4141 gctggaactg tgaagcctga cgtaaagaaa ttgctgatcc tgaacctccc gtatctgatt
     4201 tttgtgtacc tgtttgcgaa atgcggggag gcgtaccggc tggccgctgg tgctgacctg
     4261 tcggggaagc tcctgcattt tgcggatggt tttgcggcgg cttttgccaa cccgctgcca
     4321 agcctgcacc tgtttgacct gtgtatcggt atcgccggtg ccgtgatcgt gcggctggtg
     4381 gtgtactgca agagcaagaa cgtgaagaaa taccgcaaag gcatggagta tggcagcgcc
     4441 cgctggggga cggccaaaga catagcgcct tacatcgacc ccaagtttga aaataatatc
     4501 ctgctcaccc aaacggaacg gcttaccatg accggcaggc ccaaagaccc caagacagcg
     4561 cggaataaaa acgtgctggt gatcggtggc agcggttcag ggaaaacgag atttttcgtg
     4621 aaacccagtg cgcccgtaag ggcggtataa atcctacctt gagcaagtag taggtaaaag
     4681 tatcaagcgg cattgtgccg caacctatca ttccccgtct taccccaaaa gggaaaccgg
     4741 agggcgacca tagcatgacg gaggacacgg ggcgctgaag cctcagaagc gccggcgtga
     4801 cggaatgaaa atccacgtat gaggatggaa gctagactgc ttgaatgaca gcctgaaatc
     4861 gggaccaaga gcgtacccct gacgtagaga ggttgtactc gtcagtgttc ctgacagtcg
     4921 cattgtttgg ctatgcggct gctgatactc ggagcaggtt cagccacggg aaagtggaaa
     4981 aaggcgaacg tcacataccg acaacgtgga acgcttgttt ataccgtact aaacggggat
     5041 tgcctaaagt gaaatgccga aaggctatga aaacgctgaa atgcggggtt ctgaatattc
     5101 accatggcaa cagagttccc atagtagtct gcggacggga aagccgtcta catggcaaag
     5161 gggaacagtg aatcattttc aaaacagaaa ggatggtgtg tgagacacta tgagaaatcc
     5221 gattcatgtc ttgaaaagcc ttgaagaaaa ggcaagtgta agtaactaca aatatgaaag
     5281 attatatcgc aacctataca atccagagtt ctatctcctt gcatatgcga acattgcgaa
     5341 atcgcagggg agcatgacgc agggggtaga cgggcagacg ctggacaaca tgagcctgcc
     5401 gagaattaac cggattattg aatccatacg caacaggaca tatcagccaa agcctgcaaa
     5461 aagaaagtac attcctaaaa agaacgggaa acttcgcccg ttgggaatca cttctactga
     5521 tgataagtta gtgcaggaag tggtcagaat gattcttgaa gcaatctatg agccgacatt
     5581 cagcaacaat tcacacggtt tcagaccaaa aagaagctgt cacacggcac ttacgcaagt
     5641 gaagaaaaac ttcacaggtg ttacatggat tgtcgaggga gacattaagg cgtgctttga
     5701 taacttcgac catcatgtgt tggttgaatt actgcggaaa aggatttcag acgaggcttt
     5761 tatcggtcta atctggaagt tcctgaaagc cggatatatg gaacaatggc agtacaactg
     5821 cacctactcc ggtgttccgc agggcagcgg tatcagtccg atatgtgcaa acatctacct
     5881 cagcgaactg gacaactata tgcaggaata caaagaaaag tatgattgcg agccagaacg
     5941 cagaaggacg accagagaat acgagcgggc gtcccggaga tacagaaagg cacgtaaagc
     6001 gttaatgggc gcagaaaaat caactcctga actggtaaaa gaatttaagg attccagaag
     6061 gaagaagatg aatcagcact attacaatcc gtttgaggaa ggcttcaaga aaatccagta
     6121 caaccgttac gccgatgatt ttgtaatcgg cgttatcggc tccaaaaagg acgcagaaaa
     6181 aatcaaggaa gatgtaaaaa tctttctcca agaaaaactg catttggaaa tgtccgagga
     6241 aaagacgaaa gtcacccatt ccagtaagcc ggtacgctat ctggggtacg atttcaaagt
     6301 aatccattcc aagaacatga agcgttgtaa aaacggcgac atgaaacggg tgtggtatgg
     6361 aaaagtattc ctgtatatgc cgaaagaaaa atggattaaa aaagcgatgg aacggggagc
     6421 gatacaggtg aaacgcaaca atgacacagg caaagaaatg tggcggccta tgccaaggaa
     6481 agacctgatg aaccgcagcg acgcagagat tgtgtctact ttcaattccg aaattcgagg
     6541 gttatataac ttctatcgga tagcagaaaa cgtaggcgca ttgcacaagt attactacat
     6601 ggtgcggtac agcatgttaa aaactctggc aggaaagcac agaacgaatg tcagtgttat
     6661 taagaagcgg cacatggtaa acggagtact gagaatccca tacgatacaa ctaagggacg
     6721 taaatactgc gaattttacc atgatggatt cagaaagcac agcgacggct atgacaatgt
     6781 ggcagacgtt atgccgagtt ataggaaata tgacagcagg cacacgatag taaaccgcat
     6841 aaaagccgga gtatgcgaga tttgcgggga acatgcagac tatttatgta tgcaccacgt
     6901 aagaacgctg aaatcgctga aaggcaggga tatatttgag cagaaaatgc tgaaaatgag
     6961 aagaaaatct ctggctctct gccctgactg ctttgaactc ttacacgaaa ccaaagaatc
     7021 aaggtgattc atggaaagcc ggatacgctg agaggtgtac gtccggtttg ggaggcggct
     7081 ccctgaaacc tactgatgaa agtcagcaag gcgcagggtg cctacctcat acctgatgca
     7141 atgctttccg accacggact atccgacttc gtttgtggtg acagatccga aaggcacttt
     7201 ggttctggaa accggcagga tgttccagca ggcgggctac cgcattaaaa tcctgaatac
     7261 cattaacttt tccaagtcca tgaagtacaa cccctttgtc tacatccatt cggaaaagga
     7321 cgtgctaaag ctggtaaata ccctgatttc caacaccaag ggcgaggggg aaaagtcagc
     7381 ggaggatttt tgggtgaagt cggaacggtt gttttattcg gccctgatcg gctatatctg
     7441 gtacgaggcc ccagcggagg aaatgaactt ctcaacgctg ctggaaatga taaacgccag
     7501 tgaagcccgc gaggacgacc cggagtttca aagccccgtc gatctcatgt ttgagcgttt
     7561 ggagcagaaa gaccccgacc attttgcggt gcgccagtac aagaaattcc tgctgtccgc
     7621 aggcaagacc cgttcttcca tcctgatttc atgcggtgcc agacttgcgc cctttgacat
     7681 ccgggaggta cgcgagctga tggaggacga cgaaatggag ctggacacca tcggggacga
     7741 aaagacggtg ctgtatctca ttatgagcga tacggacacg acctttaatt tcattctggc
     7801 tatgctccaa agccagctaa tcaacctgct ttgtgaccgg gctgatgatg tgtacggcgg
     7861 gcggctgcct gtccatgtac gcctgatttt ggatgaattt gccaacattg gccaaatacc
     7921 caatttcgat aaactcattg ccaccattcg aagccgtgaa atatcggcct ctattatttt
     7981 gcagagccag tcgcagctaa aagcgattta caaggacgcc gccgagatca tttcggataa
     8041 ctgcgatagt gtgcttttcc ttagtgggcg cggcaaaaat gccaaggaga tttccgatgc
     8101 gctggggaaa gaaacgattg acagtttcaa caccagcgag aaccggggaa gccaaacgtc
     8161 ccacggattg aactatcaaa aattaggaaa ggagttgatg tcccaagacg aaattgccgt
     8221 gatggacggc ggcaagtgta tcttgcagct acggggtgtg cgcccgtttt tatcggagaa
     8281 attcgatatt accaagcatc cccggtacaa ataccttgcc gacgccgaca agaagaatac
     8341 ctttgacgtg gacagatttt tgactatcac gcgccggaaa cggcagcaag tggtaacgca
     8401 ggatgaagta ttcgacctgt atgaaatcga cctgtcggac gaggacgccg ccgctgaata
     8461 aacagcggct tttttcattc caacaacaaa atcaaaaaat cattatgagc cggaaaccgg
     8521 cagaaagagg taaatttatg gcatttttta attccgcagt tactactttg cagactatcg
     8581 tgatcggact gggcggcgct ctgtgcgtat ggggcggcat caatctgttg gagggatacg
     8641 ggcaggacaa tccgggggca aaatcgcagg gtgtcaagca cttaatgtct tctatgtgaa
     8701 aagattttaa agagaaaatg cacatcctac gcagataaca tgacaatttc atgcaaagcg
     8761 aaaggagaca ccatgaaaat cgaatacaca aaagtgggag actattattt accgaatctt
     8821 tactacccgg aggaaaccag accgattggc ttatggggaa tgcttcggaa ggaatatctg
     8881 aaagaacata aatctggaac ttatacatat cttcttctga ccgccaggct ggacagttat
     8941 ttggcagatc tgaatgaaca ggcacaggaa cgctttgaat tgatcgaaac tcagatgcga
     9001 agtgcggagg gagtttccga ggaactgaag agacagaatc ctatggaatg gctatgccac
     9061 tgtaacaata tccgcaaccg ggctgctgag atcatcaagc aggaactgat ttatgtataa
     9121 gggggattct agatatgaga agagaactga ttgaaagtta ttatgaagaa taccagcggg
     9181 agctggaaag atttcttgaa tctgacccgg agatgttcac tctggaagca gaaattcatc
     9241 attacataga aatactggaa ccggtcctca gagatggcgg acaggagatg tggcagacac
     9301 tggacaggat tatttcagcg aaaaatgcaa tggagggaat tgtggcaaaa gaaatgtata
     9361 tccggggatt tctggattat gaaagactgg tgtgtgaaaa agacagtaag atctgaataa
     9421 tcagaacata gactatatat aacgaggctt ttattgtata gacagtaaga gcctcattta
     9481 atatagtttt cataataaaa aacaggtggt agtacgtatt tgctttgtgt tatataagaa
     9541 ttggtctggc tgaacaatgg tgaaaattta ctatgatttt acttaaatct gattgaagga
     9601 tgaaaaagtg atgatatcat atagtgggtt gatagggtta cagaaaatgt attcacagag
     9661 gcagagaaga aataatgaaa gtaaaaaatg cagatgaatt ttataaaata ttagaaagaa
     9721 aggctatata tccggtattt cagccgattg taaatttaca gactggagat gtggcaggat
     9781 atgaagctct tagtcgtatc gacaggcatg atacgactct tatgatttca gatttgtttg
     9841 tgattgctga acaggtaggc tgtgtttgga aattggaaaa attgtgcaga aataaagcat
     9901 tgaaggcagc ggcgaataaa ccagaacatg caaaattatt tcttaatgta gatggtaata
     9961 tcattcagga taaatcgttc atacagggat ttacaaaccg taaggctgcg aaagccggag
    10021 taccgtcctg tgacattgta tttgaaataa cggaacggtc ggatatagaa aactatcaga
    10081 ttctgcaaca gattatgaag cattatgcag atcagggata tgaaatagct ttggatgatg
    10141 ttggagctgg atattctggt ctgaaccgtg tagtaaatac atcgccaaac tacttaaaag
    10201 ttgatataga actggtcaga gatattcaaa aacataagaa aaaggaaatt atgatggaat
    10261 ttctgttgca ttactgtaat gagacaggag caattctgat tgcagaaggg atagaaacag
    10321 aagcagagtt ggagtgtctg tgtagattag gtgttcatta cggacaagga tattttcttg
    10381 gaagaccgga cagaacgttc aaaaatgtaa ggaatgaggc gatgttggta ttacgtagac
    10441 tgaataataa atcgtaatga aaactatatg tcagcaggtg aaaaatgaat aagtcaatta
    10501 taacattata aagatttcca ttgattttta cacctatttt acacaagcac tcacatagat
    10561 ttgatttctg tcttctataa ttgtatagga aaatgtagat cttatataat aacaggagag
    10621 gaaagagaaa tgaacgagaa tgtgaaagtg gtttttggtc tgatcggtgg ccttgccttg
    10681 ttcctttatg gaatgaacag catgagtgat gcacttcaga aagccgcagg agaacgaatg
    10741 aaaaaaatcc ttggatttct tacaagaaat ccaattatgg gagcacttgc cggagcactt
    10801 gtaacagctg tattgcagag cagttcagcg acgacagtta tggttatcgg ttttgtcagt
    10861 gcgggactta tgagtcttcc gcaggcaatt tctgttatct ttggtgccaa cataggaacc
    10921 actatgacgg cacagctgat ggcatttaaa atcagcaatt atatttatcc aattatcttt
    10981 gttggattta ttctgaactt tgttagcaaa aaagaaaagg ttaaaaatat cggtatggta
    11041 attttttcat tcggactttt atttgaagga attgaaatca tgggtgaagt catgaaacct
    11101 ttggcgggaa gtccggtctt tgttgatctg atgggaaaag tttcatccat tccggttctt
    11161 ggtgttgtac tgggagcggt catgacattg gttgtgcaga gtagttcagc tacgattgca
    11221 gttctgcaga attttgcatc acaggcagga ccggacggag taagcagtgt aatagggctt
    11281 accggtgcga ttccgattct tcttggagat aatataggta caacgatcac agcacttctt
    11341 gcatcaattg gacaatctaa aaatgcaaaa cgtacagcaa ttgcgcatag tatttttaat
    11401 atcagtggaa gctgcgtttt tattttcctg gttccgtggt ttgcaaaatt tgtccagttt
    11461 atttcaccaa agggaaatga gattgatgtg atttcaagac agattgcaaa tgcacataca
    11521 acgtttaata tcgtctgtac actggtctgg ctgccactga ttccgctgat ggtaaaaatt
    11581 gttacaacaa tcatccgtgg aaatgacaaa acagagaaag cagcttttga acccaaatat
    11641 ctggatatga aagtgattga acagccggcg gctgctatgg ttctggtatc taaggaactg
    11701 aaccgacttg gagaactggc agaatctctg ttaagtgatt taaaaacggc gattgtagca
    11761 gatggagact caaaaactca tggttcgttt atagaaaatc tggagattgt acatcagtta
    11821 caggacagtg tgacagaata tattaccaga ttattcgcaa gcggaaacct tacagaacaa
    11881 cagtctgagc agacggcagg gttactgtat gtaaacaata gtattcagag gattgcagac
    11941 agatgtgaag atattgacca gatatgtgaa aaagtaaata acaatggaaa aagactgacc
    12001 agtgaagcct cctcagaaat ggaagactgc attgatatta tccagcagct tttaaagaaa
    12061 gcaatggagg caatccgtaa gggtgatgct gtagtggctg aaacagtttt taaaaataag
    12121 aaaaaaatgc ataaagcaga gaaaaaatac agtaaggcac atttaaaccg tgtaaaaaat
    12181 gaggtttgtg atgcgtctat gacaagatat ttttctggga tcatgtataa ttttgaccgt
    12241 atggcagata attgtgtcag cattgcagaa gaagcctgtg ataatgtggc ttttataaac
    12301 cttgatgaag agacgggaaa atcaatgatg gaaagaggtg cagtataaaa tggtaaagaa
    12361 aaaaaacatc cgaaaatctg cacttctgct gataatgata atgctggcaa cgggaataac
    12421 agcatgtgga accgaggaag agccattgcc ggatctgtct gctatgggag ctgtcacagc
    12481 tgtatcacga gaagaagggt caggaaccag agcagaattt gagaatcttt tgaaactgcc
    12541 tgagagtgat acaggaatag ttgttgattc tacagagaaa gttctgaaaa aggtggaaga
    12601 agataaaaac gcagttggtt atgtcgcata tagttccgca acagatacga atggaaaaat
    12661 acttcaaatc aacggagttc ttccttctga gaaaacaata gacaataaca gttatcctct
    12721 ttgcagggat tattatctgg cttataatgg agaactgaca gatgtggaac aggatttcct
    12781 gacttatgta aagagtaaag gacaggatat tgtgaaacaa tattgtattc aggctgacag
    12841 tacaacaaca ttcctttctg acaagtcaga aggaaagatt ctgattgaag gatccacttc
    12901 aatggaacca atggtaaaag cactggcaga tgactatcag aaacaaaatc caaatgctga
    12961 aatagaagta aaagcaacag attcttcaag agggattacg gcggtgataa gtggagagtg
    13021 tgatttcgca atgtcatcca gagaattaaa ggattatgaa gcagagcttc tggaaacaaa
    13081 agtcattgga aaagatgcta tagctattgt gataaatgaa gaaaatccac tggaaaatct
    13141 tacaataaaa caacttacag gtctgtacaa tggaacatat aaaaactggg atgatctttc
    13201 ctgagaaaaa cgaatataat atcttacaag cactgactat aacggtcggt gcttcttttt
    13261 tggatttttt caaaattatc tgacattctt caaattccca cgtgtgatta gtggaaaaat
    13321 aattttttct gcaaaagtac cgtccaaatg ccctctgaaa cttgctatta gtgagggaat
    13381 atttcattta tgcgaaaaaa acttgcaaaa gggggttggt aaataccgct tcccaggctt
    13441 tataggtaga ggaacaatta ttttaaaaat tttaccccaa agatgtccgc ttttgcctgt
    13501 ctcagtacag agtatgtgaa gggactaaca aaagtaaaag aaaaaatgat aaaaatgcaa
    13561 aaaaatatca cgaatctttt gcatcttgcg aagtcatttg actctagtaa tcagaggacg
    13621 ttttggaatg ttccttcgat gaaccttgac aaccgataag tgcaccctag aagcctaaca
    13681 ggagttatgc catgaccttt ctttttctga gagcgagcga aattataatg acactcccga
    13741 tgatgggcgg ctgaccgaag ccttatggtc agtgacctgc tgatgcaggc agaggcagtc
    13801 tgagacggct gcaggtgaca gggaaacgca cgccagaccg ccggacgaat gaaactgatg
    13861 acaatgttta cagggcaaaa tgcccggaaa ggagcagttt atgacagaac tgaaaatgaa
    13921 agaaatgcag ttgattaact ggtttggatg cccgaatctg atttatacaa gggagagaat
    13981 ccatctggtt gccatgctta caacagatga agaaatgaaa aagaatcttt acagggtatg
    14041 cagattcctt gcaagagaag aaatcgcata tcggtatccg atcatgtatg aaatgatccg
    14101 gaaaatattt tcaccggatg ataccaatcc accggaagca tgtgcaatgt ttatgccgga
    14161 cgatgaaaca gatgatgaag aatatgcaat ggcagcgtag ctgcggaaag gacagtaacg
    14221 attatgaata aagtggtttt aatgggaaga ctgacaaaga atccggagat caaatatgcc
    14281 ggaaaagata atgatatggc agtagcaaga tatacactgg cggtaaacag aagatataaa
    14341 cgtgacggtg aacaggaagc ggattttatt tcctgtgtca cattcggaaa gagtgcagag
    14401 tttgcacaga aatatcttca caagggaatg aggattgtga ttggcggtcg tatcagcact
    14461 ggcaattata aggacaagga cgggaaaaca atttatacta cagatgtgat cgtggaagaa
    14521 catgaatttg cccagaacaa ggataatggt gttggagctg attcatcgga aacaccgaaa
    14581 acagataagg acggatttat ggaagcaccg gaaggtgagg ttccattcga ctaaaccgaa
    14641 gtaaatggtt ggagaggttg ccctttgtcg gggcatcctc ttttccattt tacagaagac
    14701 atcaaaaatt acaggtattc aacctaacga aatcgtgata taatcaaaat atgttcagat
    14761 gaaaaatatg gaggttgctt atgggcgtaa gttataacag attatggaaa atgctcatag
    14821 ataaaaatat gaagcggata gagatgcagt acctcaccgg gatcagtgga aatattcttg
    14881 ccaggatggg aaaaaaccag tatgtatcaa tggagacgat tgagaagatc tgcaagaagc
    14941 tggactgcac cgttgatgag atgctggaat ttacagatga tgagtaacaa gagcagacat
    15001 gtcaaattat taaaactgga agaatgcttc agaagtcgaa aaaagaaatt gaaaaaagat
    15061 aacacgccgg atacggcata aatctcttag caacgctaag agcgcacttc tatacaggca
    15121 attccatctg cctgtatcgt gcgacctgcg gtgctgttcc cgatttcaag aaatctcaaa
    15181 ccgggaaata cagcgaaatc agagatttcg tacaagggga agatcccctt gcggcacaga
    15241 tgcctatccc tgataaagtt tgtgactgaa tatttcagat atagacatat aaggggaaag
    15301 aaagtgtgaa atcaaaattt gtggagagat tttatgatta gaaaagcaat actaaaagat
    15361 gttaattcga ttgaagatac ttacaatgag catttccagt atgaactgaa tcatacagcc
    15421 tttactgtat ttaagaaagg ggtttatcca acgaaagatg atgcagaacg ggctatttat
    15481 gctggtgctt tgtttgtata tgaagaaaac ggaacaattg ttggaagtat cattattgat
    15541 aaagttcaac caatagaata tgcaactatt ccttggaaag aaaaattatc tgaagatgag
    15601 gtaatggtaa ttcacctttt aatggtacgt ccgagtatgt ctggtaaagg aattgcatca
    15661 tcactaataa aattcgctac agaattagca caaaaaaata gttgcagggc actacgtttg
    15721 gatacaggaa gtcaaaatat tcctgcgcta tctttgtatc aaaaaaatgg atttgaaata
    15781 attgctagtg cttctaaaaa agttggagat gtaattgcac acaaaaacca tctttttctc
    15841 gaaaagatat tgtagttcgt aaattccagt ttgaaaaatt gagaaaatcg gagtttgagg
    15901 agcatcaccg ttatgattag agaattacgg aaagtggata taaataaagt tgcagaaata
    15961 tggttagata caaatataaa gacacattat tttatctctg ctcaatattg gaaaagtaat
    16021 tttgagttag taaaagaact tctattacag gcaactgtct atgtttacga ggataaacag
    16081 gaaatacagg gttttatagg gttaagtaac gaatacattg aaggtatttt tgtttctgcc
    16141 gaaatgcaat ctcaaggtat aggtaaaatt ttgctaaact atgtaaaggg aaaaagaaac
    16201 aaattaatct taaatgtata tcaaaaaaat acacgggcaa tatcctttta tcaaagagaa
    16261 ggatttgaga ttcagtatag cggtttggat gaagctactg gagaaaaaga ctatgtaatg
    16321 gcatggcagc agaaatagaa attcaagttt tagtgtttgg aaaattatga cttagtgtgc
    16381 aagttgggag aaaatatggt aacggaaaaa gagaaaaaag catgtcgatt tattgccgaa
    16441 tggctataag attgtgagac agtctctggt agatgatctg gagggcgaaa aaaaggtatg
    16501 ttgggagaca gaaattttgc aggatgatga tagtggattt acttatgaaa tcagcaggtg
    16561 tctgtatttc gatacctgta aggaacatgg ctatgcagag ttctgcaaag tattttgcaa
    16621 ccatgactgg tatgcttatg gagtattaaa gcatcattcg agatttatca gaaaatcgac
    16681 gattgccgaa gatggaactg tatgtcatga tacaatcgag aaagtaacag agaaacattt
    16741 tttataaaac cgaaaaattg attatgggag gaaattgaaa atggcgaaag aagagagatt
    16801 tgaaaaaata tattctcagg gagcattaac tactactgag atatgggtgg ataaggaaac
    16861 tggtgtgaat tatttatatc atgcatgtgg gtattcaggt ggactgactc cattgttgga
    16921 cagagatggg aaaccagtta tttctcaggc tataaataga tgaccttaga aacagcaact
    16981 gttatggtaa gtaacagcat cgtcacaata tgtatatggt cgattggtgt gtgcggtaat
    17041 atactatatt cagaaaaaca attcaggaga tttttatgag aagatcaggt tggacaaaat
    17101 ggttgatcgt gattccggca atgctaggca ttgtgttggt gacctatgtg cggtatacaa
    17161 cggatatctg gggatttggt gcatttatct gccagctgat tgcaattttg gaattggctt
    17221 atggtctgcg aattgcgatg ctggcacaga atagaaagaa gtcctatcgg ttaacaccgg
    17281 aagaacgaca tgagtatgca cggtatttat atgagaagca atatcaccgt taccctgctg
    17341 tagcgaatca gatgcttttg gttatggcac ggatgagtgt actccttaac aattatgaac
    17401 gggctgcaca ggagctggca gatattcgta tagataaatt caatccagca cagttgaagt
    17461 tgtattatta cctgaaagtg gttacagcaa tggcagcagg agatgcgaca gggattcaag
    17521 agagccagat gtgttacgca gggattccgg atactaaatg cacatatccg tcaaaagcag
    17581 agcttgctat gtggatagag cagagagcgg cagcgcagat ggcagaggca ctgaagaagg
    17641 cagtgccgga taaaaaagag catccgatac ggatcagtat cattactttg gttcttgcgt
    17701 atagtacctt cttctatgga ttgtggtatg gtataaaacg tgatatggga tatgaagtga
    17761 gatatcgttt cgcagaaata tcgattactg ttattacaat cagtctggcg gttcttttga
    17821 tctgggggat cgtgagttta tacagacaca attgccaggc attatccata caattcggta
    17881 aaaacaaagt ggcattgata attgtctatg gagctgctgc gatatttgca cttttgatta
    17941 ttggaatgag tggtctgagt gtagtgttcg gaatggatgg aacagaaacg gtgactggtt
    18001 gtgacagcaa atatacgtat ataactgtgc aggaagatta tggtggtgaa cggagataca
    18061 gaaccaataa tccatttgtt atgcagagta tggatggtct gtggtggtct tcggatacaa
    18121 tagatgaaga agataatcaa gaagaggata ctacagaatt accggatttg gatacagaga
    18181 attcaacaga ttcagaggat attcagagtg gaaataccat agaagattac gagacagaag
    18241 ggcagcatga tggtctgaca attcaaaatg caatgctggc agtttataat taccttcagg
    18301 agcagaatac acttcaaaat atggcgttca gttacacggc gaatgcaaag ggagaagtct
    18361 atgctgtcgt cagcgaaacg caggaaacaa agaacggtaa tacggtaaat gtgagatact
    18421 gtctgtacga taatggttcc aagacagatg cagatggtaa ttcctgcgaa gaactggtgt
    18481 tagaaaaagt atatccggat ggcaattacg agaccgaatt ggtagatttt tatctggtaa
    18541 atgcagatac gatgcaggtg acagacgaac agaagaatac gtggtaaaat aaattctggg
    18601 gaagtaggtg cattgtatga caatgagaga aaaaataaaa ctattatcgt aaatgtttca
    18661 agcggactgg gttcttttgg aagtatgcaa aaggactcca agacataaga ggtgaactgc
    18721 gctgatccag gtgccacggc aacagatttg aatgtcgacg gaggaaagca aacagtacag
    18781 gcgcaatcgt aaaacttgca atgattgata aaaccggact aacagtaact tttattagtc
    18841 gatttggaaa tatgccttgg taatcaacaa ttccggtttt actgactaag aaaatgagga
    18901 ggattcataa tgagtgagaa aacagagatt acttttatgc aaacaagact aatccggctt
    18961 gcttcagaag agtggcattt acctgttgaa cagattatcc acttgtttaa agaagccgat
    19021 gttcttggat atatagaaaa atgctatggg atttttcatt gtgaaggcga tgaagctgtg
    19081 cttgaagata ttactgaatt tttgcaaaga aaggggatag agattagtgc ttgagttaaa
    19141 agatggattt gttctctatc atggaagtta ttgtgaagta aaagagccag atcttgccaa
    19201 atgtgcaaag agaaaagatt ttggacaggg attttattta accacatcaa aggaacaggc
    19261 agaaagtttt ctgagaacct caatagccaa agccatcgca accggaacaa ttgaagaggg
    19321 gcagaagttt ggatatattt caacttttga attcaaactt tcagaaaatc ttgaaacgca
    19381 tatttttgaa aatgcagatg tggactggtt acattgtatc gccgctcacc gaaagaaaaa
    19441 aatgtttatt gaggttgaac gtgaaatggc aaaatatgat atcattgcag gaaagattgc
    19501 ggatgatgca acaaacgcta ctcttaccgc ttatcttgcg ggtgcttttg ggactgcagg
    19561 tgataaagta gcagacgatt tctgtatcag acagttgctg ccgaataaat tgaaagatca
    19621 atactgcttt aaaactgagg cagctattga gtgtctgaaa tttgtgaaag gtgagaagat
    19681 atggctgaag taacaattcc aaaggaaaaa atgaattata caatcgacct tctgatcact
    19741 atggtgacag atgaaatcgc agaagagaca ggcaaggaca gaaacgaggt attgacagat
    19801 tttttgtgtt caaagacagg aaaagcactg tatgatgaga agacaaagct gtggtgcact
    19861 ggtccggcat atatagctga actgtatatg gaagaattaa agaaatcctg atatgggatg
    19921 tgagtttaaa tttatacgac aatggtgaca aagactttca ttccaaggag caacaattaa
    19981 atatttcaaa aacgctgagg tcagcatttc ctaatcacag gacaacgacc tcagtttttt
    20041 atacccagga atcggcattc atgaataagc cggttctttt tgtttacaga aaaaagaagg
    20101 agtacaagaa aatgagcaga gcaaaaaatc caaagtatga atccatgtca ttggaggaac
    20161 tgaaggccaa catggaaaag agcagacagg aactggaggc agcaattcac cacaaaaaca
    20221 ttctggagca gcggaagaag ctggtagaac gcagggaacg ttcccaccgg ctgattgtca
    20281 aaggggcaga gtttgagaaa gtatttccgc tgtccagaga tctggaacag gaagacgtcc
    20341 aggacatgat gaatcaactg cagaacagca gttacaacaa gagtattgtg cggcaggctc
    20401 atatagccgc cttacacaaa gatcaacagc agattgcaga agcagtaaaa aagacagaga
    20461 agggagatga cagttaatgg cactgtatca tttttcagta aggaatgtat ccagagggaa
    20521 agggcagatg gtggtcgctt ccgcagccta tatttccggg cagaggattt ttgacagtta
    20581 ttacaacaag atacacgatt atacgagcaa gtcaggtgtg atatttacag aaatcctgac
    20641 accggagtat gtgccagagc gtctgactga cagggaaaca ctctggaacg aggtggagca
    20701 tgtggaacgg aacaacaagg cacagcttgc ctattcgttt gacattgcac tgcagaatga
    20761 attgacactt gaggaaaata tcagactggc aaaagagttc tgccaggagc agtttgtggc
    20821 acgtggcatg atcgtggatc tggcagtcca tgagggaaaa gcggaaaatg aagaagaacc
    20881 ggataatccc catttccatg tgcttgtccc aatccgtcct tttacagagg aaggaatctg
    20941 gggcaataag cagagaaggg aatatattct tgatgaggat ggaaaccggg tgaaggatgc
    21001 caaaggaaaa gatatgttca atgctgtttc tactaccgga tggaatgatc cggagctttt
    21061 aaaagaatgg cggcgggcat ggacagagaa agtaaatggg aaattcaggg agtgccacat
    21121 ggcatccaga atcgaccacc gttcctacaa agaacagggg attgacctga tcccgaccat
    21181 ccatgaggga tatgaagtcc gggcaatgga gaaaaaggga atcaaaacgg tgatcggaga
    21241 attgaaccgg gcaatccggc agtttaacca gatgtttatt tctctgaaag aatccattca
    21301 gtggatgaag acagcttatg aggaaatgaa agcagagctg gatcgcaggc agaacccgac
    21361 actgctggaa agcctgcagg attattatga taaaaagaca cagggcagac caccacttcc
    21421 caatttttat gcggagatga aacagagagg taagaatctc tccaatttac aggagtttgc
    21481 aaaatccatc agttatcttc agacacatca gatagaaacg atggatgact tgcaggaacg
    21541 gattgaggaa ctgaacggtg ttgtttctgt cagtaaaaaa gaaatttctg aaaagcggaa
    21601 acagttaaag gaacttgaga atctggaaaa gatggcagag gtaataaaaa caaatcagcc
    21661 attgattgat gagtataacc atttctattt tcagaaaaga cgggagagat attatcagca
    21721 gcataaaaaa gaaatcaatt attaccgcaa atgtgaacgg gaactgaaac aacatctgga
    21781 taaaaatgga aaagtgccaa ctgccagatg gaaacgggag aaagaagaac ttcaggcggg
    21841 gatcgaagag ctgaaagcag ataatcagcc gtaccaggag gaactaactt ttgtgaaaaa
    21901 ggtacagagc tgtgcggata ttgccagacg tgaccgggaa atggcagaag cagatacttc
    21961 cgggcgttca gaagaaaaaa gggagaagca ggtgaagttc ccgacatttt acacagttca
    22021 ggcgaaagag attcttgaag aaaacgggaa agcagagcaa cagccgttga accagtcgga
    22081 acagaatcca gaaaagaaga cctctcttct gagaaaactg gatgaaaaaa agaaagagtg
    22141 tgcagagcgt gatgcaaaac aacaggcagt gaagaaaaag cgaaactatg acatgagttt
    22201 ataacagtct gtcagcagga cagacgggaa aggatgagca aaatgcagta tacagaagaa
    22261 caaattataa gagcaaatca gacagatctt gtttcttttc tgagcgcaca gggagaacaa
    22321 ctagggaaaa gcggaaagga ataccgttgg aaaaagcatg acagtgtaac ggtatccgga
    22381 aaccggtggt acaggcacag tcaggggagg ggtggctatc cggtggattt tgtgatggaa
    22441 ttttacaatg ccacatttcc ggaagctgtc aagatgctaa ccggggagga aggcgaaggc
    22501 aggaacagta cctgccccgc cccgtcccct gacttccgat taccagagaa agaggaaaat
    22561 aatgatagaa tcatcagata tctgacagaa aacagaggga ttgaaaagaa tatggtggag
    22621 gaatggatcg gcagtgggga tatttatgaa gaaaagaagc accataatgt ggtgtttgtt
    22681 ggcagagatg cagacggtat tccaagatat gcccattgca ggggaactgg tgaaacaaaa
    22741 taccggggag atgtggctga atctgataaa tcttacggat tctgtcacag agggacggat
    22801 aatcagttgt ttgtgtttga agcagccatt gatctgcttt cttttatcca attatttccc
    22861 aaagactgga aaaagcggag ctacctgagt ctgggtggca tttccagtgc ggcactgatg
    22921 gcttttcttt ctgaacgccc tcagattact tcggttttcc tgtgcctgga taatgaccat
    22981 gcagggaatg aagccagtga gaaattggca atagaaatcc cggacggata cagtgtcatc
    23041 cgtctgaaac cgtcccgaaa ggactggaat gaaatcctct gtgataaaaa tgcagacagg
    23101 aagaaatcaa tcattgaaac agttacaatg aaagtaccgg agaaggaaga actggtgccg
    23161 atgttgtgtt atgaagacat tgaacagacc agtgtagaat ggctgtggtt tccctatctg
    23221 ccatttggaa aactgacgat tattcagggc aatccaggag agggaaaaac ttattttgcc
    23281 atgatgctga ccgcagcctg cacgaaccgt aagactttcc cgaacatgga agagattgaa
    23341 ccttttaatg tgatctatca gactgcagaa gacggtatgg gagatacgat taagccgagg
    23401 ctggtggaag caggagcaga cctgagtcgg gtgatggtca ttgatgatac agaggaagca
    23461 ttgacgttat ccgatgaccg tattgaaaaa gcaatccgtc agaatcaggt cagactttta
    23521 atcattgatc cggtacaggc ttttatcggt gccgatgtgg atatgaaccg ggcaaatgaa
    23581 gtccgtccgg tattccggaa actgggaatg atcgctgaaa agaccggttg tgcgattgtg
    23641 ctgatcggac atctgaataa atcttcaggg acacagagta cttaccgggg acttggttct
    23701 attgatatca tggcggcggt gagaagcctg ctttttatcg ggaaagtaaa gaaagacccg
    23761 acaacaagag tactgatcca tgagaaaagt tctcttgcac ctccaggtga gacgatggca
    23821 tttaaacttg gagatgaaga aggattccgc tgggtgggag cttatgagat ttccgcagat
    23881 gatcttctgg acggaaagga aggaaagccg acagaaacaa aactgcaacg gggaacgaaa
    23941 ctgatctatg aactgcttgc agacggaaat gcagtcacaa tccgggaact ggatgaaaag
    24001 gcaaaagcac agggaatctc ccagagaacc atgcgagaag ccagaagccg gatgaaagaa
    24061 gaactggatt atcggatgaa tgaaaagcag gaaaatacca ttcgattaaa aaaacaaggg
    24121 agaatgggtg acggcagaat tttggagtga acggcaggct ttatagatac cctatctgcc
    24181 gtctgccgtc tgccggtgca aggcaatcaa acggaaatag caggtatcca tacagaataa
    24241 ggattgctgt gaatctggaa caaaaatgaa ttgtaatatg gcggatataa tggtatgatt
    24301 atccggtaac agcaatgtta cggggaagca cctgtgacag ggtgtggctg cctcctgata
    24361 tttacagacc ggagcctttc ggggttctga gatgaaagaa aggaggtgcg tgatatgaca
    24421 gcttatgaga ttatctcaat attcttaggc gtactcagtt tactgatcgc ttttggtagc
    24481 ctgttaattg cgattattgc ctttcttgat cggaagaaca agaggaaata aaaatgccta
    24541 tcctgtctgc caacaggata ggcggtggtc gtaagaccat ttagtcaact caggcggcat
    24601 ccactttgga gtgggtgctt ttcctataac aaatgtacca cacattcctc ttgttttcaa
    24661 tgggaaaatt aaaatatgca gttcatctga cagggattta tccctgtctt tattatatcc
    24721 ccaatttctg aaatgttaat acaaaaattt taatatatgt aaccaacagt ctgggcagtt
    24781 aatctgtaaa aatggattag ctgctctttt tttgcaaaaa atggaggttt tacaatggca
    24841 gtgaagaaga aaattatcaa catcagagta tattttgacg gtgatcagga cgcaaaagaa
    24901 gtgtttgcag aggtgatcgc acagaaaata cgtgacacga aaacaaaaga taaacttgca
    24961 ataatgaaag ataagcagta taatgattat gagttcagta atctgaaatc tgcgtaggat
    25021 gtgcaaagta acggaggtat cagatgaaca acagatatat gagtaacaat atagaaagaa
    25081 atgcaacctg gagagttggt gtatactgcc gactttccaa agatgacgaa ttgaccggag
    25141 agagtgccag catctccaac cagagagaca tactggtgaa ttactgtcag tcacagggct
    25201 ggcaggtggt ggatgtgttt caggacgatg gttatacagg attgaacaca gaccgacctg
    25261 atttgcagag acttctcaaa gcctgtgaaa aaggactggt gaatcttgta attaccaaag
    25321 accagagccg actgggacga aaccatgtac agacaggatt tctgatggag gagtttttcc
    25381 caaagcatgg agtaaggtat atcgcattat atgataatgt ggataccttt gccggagaca
    25441 atgagattgc cccatttaag aacgtgctga acgagatgta ttccagagat atttccaaga
    25501 aagtccatgc gtcctatcac ctgcaggcaa ccaagggaaa gtttacaggt gttgttccac
    25561 cactgggata tcagaaagac ccggaggaaa agggacatct cctgattgac gacgagacag
    25621 caccgattgt ccggaaaatt ttccagtggg cggtggacgg tcatgggatt aactttatat
    25681 ccagagaact ggaacgccag cagattccct gcccgtcatg gtggcaccgg aaacggggac
    25741 tccgcaccta ttacacaaaa tgggaaaaga aagacccgga aaatgggaaa tatgtatggg
    25801 atgaatccta tctgaaatcc ctgctgatga atcccgttta ctgtggtgac atggcttccc
    25861 agaaaaggta ctaccgtttc aagattggca atcagggaga taaagctccg gaggactgga
    25921 tcacagtcag ggatacccac gaagcaatca ttccgcagga tgtatttgat ctggtacagg
    25981 caaagatgaa aggcaggaaa cgccagacag aacagggaga atacagcatg tttgccggat
    26041 tactccgctg tgcagagtgt ggcggtgcgt tgacgttgaa gaagacgcac accaaagacc
    26101 agcatgaagt ttatacctgt tccacttata tccataaggg gaaagcacat tgtacccagc
    26161 accgggtgga cgctgatgac ctgtatgatg cagtccttac cagaatccag gaatgtgcaa
    26221 aagcagttac cggagacgga acagaactgg aaaaccgggt aaaggagtta tgcgaggaag
    26281 atacacaggg acacagggaa agtctggaga agctggtatc aaagcagaaa gaccgcctgg
    26341 aaacgttaga ccggctgatt gcaaaactgt atgatgacct tatcaatgac aagattacag
    26401 aatctgtttt tgataagatg ctggagaaga cacagaagga gcagacagat atcaaaaagg
    26461 aacttttcca gaatgagagt gtgctgaata cggaagaaaa actggatgca cagtcccagc
    26521 agtggattga cgatattagc gagtatgcgg acatcaaaga actggatgcc aatcttctga
    26581 accggctgat cagtaaaatc gttatctcag aaccgcagga gaaagacgga acagaaaatt
    26641 actgccgtac tcctgaaacg gtcacaatgg aaattcattt caacttaaaa ccaataccgg
    26701 agcttggaac catcgaaaga ggttctggct cccataaata agaaaaatct atcaaaattt
    26761 aatctatttt aatcttttca aggctcattt cgagcaaata ttttctcata aaagacataa
    26821 aacagcttgt cgctggcggc ggcgtggccc tgatcggtgt cactctggtt cccctgctgt
    26881 ccgggctgct gggctgatcc tggcctttgt ccctgtccaa acccttaact aatcgcctcc
    26941 gcgtcctccc ggccagaccg ggagggcgcg ggaaaggagg tgctacccgt gattagtgaa
    27001 atcattgaag aatggataaa aggcatcctc atggacggta tcacgggcaa cctgtccggg
    27061 ctgttcgata cggtaaatgc caaagtcgga gaaatcgccg ccgacgtggg acgaaccccg
    27121 caggcatgga acagcggtat tttcaatatg ctgcacagcc tgtcggaaac cgtgatcgtc
    27181 cccatagccg gggccatact cgcccttgtc atgtgctatg agcttatcca gatgataacg
    27241 gaaaagaaca atatgcacga ctttgacagc tccatgttct tccgctggat attcaaatcc
    27301 gcgtttgcaa tcctgatcgt aacgaacaca tggaacatcg tcatgggcgt attcgacgcc
    27361 acccaaagcg tcgtcaatca aagcgccggt gtgattatcg ggcagacatc cattgatttt
    27421 aacaccctga taccagacct tgaaagccaa ttggaggcta tgagcgtggg cgctcttttg
    27481 gggctgtggt tccaaaccct tgttgtgggc ctcaccatga atatcctttc catctgcatt
    27541 ttccttgtga cctatggccg catggtggaa atctatgttg tcacgtcgct ggggcctatc
    27601 ccccttgcca caatgggcaa cgcggaatgg cgcggcatgg gccaagggta tctgaaatcc
    27661 ctgctggcgc tgggcttcca agccttttta atcatggttg tggtgggtat ctacgcggtg
    27721 ctgatacaga atatcggttc cgcttccgac atatccggcg caatctgggg ctgtatgggc
    27781 tatacggtgc tgctgtgctt ctgcctgttc aagaccggca gtatatcaaa ggccgtattc
    27841 ggggcgcatt gatcccaaaa tccaagcata cacaggagga acttgtttat ttcaacgtac
    27901 aacatcattt acgctgaccc gccgtggcgg tatgactgca aaaacgtaca gggcgcagcg
    27961 gaaaatcact attccaccat gagcattgac gagctgtgcg ccctgccggt ggaacggctg
    28021 gcgtccaaag actgcctgct gtttttgtgg gccacattcc ccatgctgcc ggaagctctg
    28081 cggttaatca aagcgtgggg cttttcattc aagaccgtgg cttttgtatg gctgaaacag
    28141 aaccgtaaaa gccttacatg gttttacggg ctgggccgct ggacaagggg aaacgcggaa
    28201 atctgcctgc tggcaacaca cggcaagccg aagcgtcgtt ctgcctctgt ccatcagttt
    28261 attatcagcc cgattgaaca acacagcaaa aagccggatg tgacccgtga aaaaatcgta
    28321 gagctggcgg gcgatttgcc ccgcgtggag ctgtttgcca gacagaaaac ccccggctgg
    28381 gacgtgtggg ggaacgaggt ggaatgtgac cttatcctga cgtgaaagga ggtgccaaat
    28441 ggcttatgta accgtcccca aggacttaac ccatgtgaaa agcaaggttg tgtttgggct
    28501 taccaaacgg caactgatct gtttttccgg ggcgctggtg atcggcgtcc ccttatattt
    28561 tctcacccgt gactatctta ccaacagcgc ggcggccctg ctcatggtat ttgccatgct
    28621 gccgggcttt ttgcttgcca tgtacgagcg tcacggccag ccccttgaag tggtgattat
    28681 gcagatcatc cagtgctgct tcatccagcc aaaggagcgt ccctatcaga ccgacaacac
    28741 ctacaccgcc cttgtgcggc aataccagat ggaacaggag gtaaaggcca ttgtccaaaa
    28801 aggaaatccc aagagaaacc gaaaaaacaa agctgacccg cgcccagcga aaagaaattg
    28861 acgcggtaat ccgtaaatat aagggcgacg gcaggcccca cacggcgcag cagagcatcc
    28921 cctacgaggt catgtacccg gacggcgtgt gccgggtatc gcccggcgtc ttttccaagt
    28981 gcattgagtt tgcggacatc agctatcagc ttgcccagcc ggacacgcag acggctatct
    29041 ttgaaaaact gtgcgacctg tataactatg tggacgcctc cattcatatc cagttttctt
    29101 tcctcaatcg gaaagttgac ccggtgcagt acgccaagag ctttgagatc gcgccgcagg
    29161 gggacgactt cgacgacatc cgggccgagt acaccggcat tctgcaaaag cagcttgcaa
    29221 acggcaacaa cggcatggta aagaccaaat acctgacatt caccatcgag gccgaaagcg
    29281 tgaaagcggc gcgagccaga ttgaagcgca tcggctttga cctgttaggc tatttcaaga
    29341 gcatgggggc cgtagcccat gtgatggacg gctgggaacg gctgaacctg ctgcatggcg
    29401 tgtaccaccc ggacggcgag atattcaatt ttgactggaa gtggctggcc ccgtccggcc
    29461 tctccaccaa ggattttatt gccccgtcct ccctgtgctt cggcaatgca aagacattcg
    29521 gcatgggcgg caaatacggc gcggtaagtt tcctgcaaat cctgtccccg gagctgtcgg
    29581 acgatatgct ggccgacttc ctcaatacgg aaagcggtgt tttagtcaat ctccatgtgc
    29641 aggccattga acagaccaag gcgatcaaga ccatcaagcg caaaattacc gaccttgacg
    29701 ctatgaagat tgcggagcag aaaaaggccg tccgcagcgg ttacgatatg gacattcttc
    29761 ccagcgacct tgccacctac ggcgaggacg ccaagaagct gctgacaaag ttgcagaccc
    29821 gcaatgaacg gctgttccag ttgacgttcc ttgtcctcaa cgtggccgac accaagcaaa
    29881 agctgaataa cgacgtgttc caagccgcag gcgtggccca aaagcataac tgtccccttg
    29941 tccggctgga ctaccagcag gaacagggcc ttgcaagcag cctgccgctg ggcgtcaatc
    30001 agatcaagat acagcgcagc cttacgactt ccagcgtggc cgtgttcgtc ccctttgtga
    30061 cgcaggagct ttttcagggc ggcgcggcca tgtattacgg catcaacgcc aagtcccgca
    30121 acatgattat gctggacagg aagcaggccc gttgccccaa tgccttgaag ctcggcaccc
    30181 ccggcagcgg caaatccatg agctgtaaat ccgagatcgt cagtgtgttc ttaacgacgc
    30241 ctgacgatat ttttatttca gacccggagg ccgaatatta cccccttgtc aaacggctcc
    30301 acggacaggt aatccggctg tcgcctacca gcaaggactt tgtgaacccg ctggacatca
    30361 acctgaatta ttcggaggac gataacccgc tggccctgaa atccgacttt gtgctgtcgt
    30421 tctgtgagct tgtcatgggc ggcaagaacg ggctggaagc cattgaaaag accgtgatag
    30481 accgggctgt gcgcgtgatc taccggccct atctggcaga cccccgcccg gagaatatgc
    30541 cgatcctgtc cgacctccac aaagcgttgt tagatcagca tgtcccggag gctgaccggg
    30601 tggcgcaggc ccttgacctg tatgtgtccg gctccctgaa cgtatttaac caccgtacca
    30661 acgtggacat cggcaaccgg cttgtgtcct ttgacattaa ggagctgggc aagcagctca
    30721 aaaagctggg gatgctcatt gtccaagacc agatatgggg gcgcgtcacg gccaaccgaa
    30781 gccaaggaaa ggccacatgg tattttgccg acgagttcca tttgcttttg aaagaggaac
    30841 agacggcggc ctattctgcg gagatttgga agcggtttag gaaatggggc ggcattccaa
    30901 caggtgccac ccagaacgtc aaagacctgc tttcctcccc ggaaattgaa aatatccttg
    30961 aaaacagcga cttcattacg ctgctcaatc aggcgtccgg ggatagaaaa atccttgcgg
    31021 aacgcttgaa tttgtccacc gaacagcaga agtacatcga caattccgaa cccggcgagg
    31081 gccttttgat ctttgaaaac gtggtgctgc cctttaccaa ccccatcccg cacaacaccc
    31141 agctttacaa gattatgacg acccgcttaa acgaggtggc gggagtatga cccggcgacg
    31201 ccacaaagga ggtgaaccac catgaaagag ctgaaagcca aggcgaaagt gacgcaggcc
    31261 atgacccgcg acgggctgac gctggaaaat caggccaccg gcgaggtgga gaacatttcc
    31321 agccgggagg ccgaacagga tttgtccgcg ccggggccgg gcggcaccgc cgataagctg
    31381 ctggaacggg ccgacgccat ccatgacggc cataaggcca agaaagccgc gaagcaggcc
    31441 ggggctgtgg ccgccgaggg ggacgctgcc ctgcaccggc catccgcccg gctccaactg
    31501 tcggaggaag aacgggccga cccggattta cagccctata tccgaaaatc ggacaaacgg
    31561 gccgataagc tggataaggc ccgcgccgcc atccccaaga agcgcgtccc ggtcaaggaa
    31621 accgtctttg acgaggcaag cggcaaagca aagaccgtcc tgcactttga caaacaggac
    31681 aaggggcctc cctccctgaa acccaatccg gcaggccgtc ctgtttccga ggtttttcta
    31741 ttcggccacg ggaaaatcca tgaagtcgag catgaaaacg tgggtgtgga gggcggccat
    31801 aagggggagg aacttgccga gcggcaggcc ggaaaagcca tccgcagcac catccggcac
    31861 cacaggctga aaccctactg ggacgcggaa aaggccgagc gcaggctatt gtctgccaac
    31921 gcggagtatt tctaccagaa atctttgcgg gataacccgg agatcgcaca ggcggccagc
    31981 aaccccattt cccggttctg gcagaaacag cagatcaagc gccagtacgc caaggccgcc
    32041 cggacagcgg gccagaccgc aggcgcggcg gcacagggcg cggccaagac agccggggcc
    32101 tccacgaaag cggccaaggc cgcagccaaa gaaagtgaac gggcggcctc ctttgtggcc
    32161 cgccactgga aaggggcgct ggccgtcctt gtgttggggc ttttgatgct gcttatcatc
    32221 ggcggcttac agtcctgcac cgccatgttc ggcagcgccg gaagcggcat cacggcgtcc
    32281 tcttactttt ccgaggatag cgatatgttg gccgccgagg acgcctacgc ggagctggaa
    32341 gccgagctgc aagactatct ggacaactac gaggccaccc atgactatga cgaataccat
    32401 tttgaccttg acgagatcag ccatgacccc tatgtgctga tttccattct gtcggcgctc
    32461 catgacggga tatttaccat cggggaggta cagggtgatt tgtccatgct ctttgacaag
    32521 caatatatcc tgacagaaaa cgtcgtggta gaaacccgtt accgcaccga aaccgataca
    32581 tggacggacg cggagggcaa cacccataca gacacctacc aagtgccgta tgactattac
    32641 atctgctacg tcacgctgga aaacttcgac ctgtcccatg tgccagtcta tatcatggac
    32701 gaggaaacgc taagtttcta ttccctgtat atgtccacgc taggaaaccg gcctgatctg
    32761 ttttccggca gccagtaccc caacgcctcc accctgacgc cgccgaccta ctacgacgta
    32821 cccccggagg ctttggagga tgccaccttt gccgccatgc tcaaagaggc ggaaaaatat
    32881 atcggttatc cctatgtgtg gggcggcagc tctcccagca ccagctttga ttgcagcggc
    32941 ttcatttcgt gggtaatcaa tcatagtgga tggaacgtgg gacggctggg ggcgcagggc
    33001 ctatataaca tctgcacccc tgtttccagt tcgcaggcca agcccggcga ccttgtattt
    33061 ttcaagggaa cctacgacac ccccggcgtg tcccacgtcg gcctgtatgt gggcaacaat
    33121 gtgatgctgc attgcgggaa cccgatttcc tacacgaacc ttacttcaaa ctactggcaa
    33181 tcccattttt acagttacgg gcgtttacct taacgccaga aaggagcttt caattatggc
    33241 aaccaaactt gaccgcatcg aaaaggacat ccagaaaacc aagtctaaga tcgcggagtt
    33301 tcagaaacag cttcgggagc tggaaacgca gaaaaccgaa caggagaatt tgcagatcat
    33361 ccagcttgtg cgcggcatga acatgaagcc ggaggaattt gccgcgttct tacgcagcgg
    33421 cgcaatgcag gccgcccctg ccgcaacccc ttaccatgaa caggaggaca atgcccatga
    33481 agaataagac cagacggatt tcccgtattc tccccgcact tctggccgtg atgctctgcg
    33541 tcacggcctt tcccgttacc gcccttgcag gcggcaatga cccggcaccg gccccgctgc
    33601 cggaggctac aaccgaaccc gctacgggcg gtttggagcc tgaaacagac gaaaccggca
    33661 ccgccacgga cgggacagaa accgaggccg aaaaagatac cggccctctg accggcaagg
    33721 acatcaccga cctgttttcc gcgctgttcg gttccaaggt gagcattgcc gccacggacg
    33781 acggcatcca gatcaccacc ggcaccgaca aaaaagaacc tgaacagacc ggcaccgtca
    33841 ccaccaacgg cggcaggctg aatgtccgca ccggcgcggg gcttgacaag accgcgttca
    33901 cccagcttcc caatgggacg acggttgagg tagtcggcac agacggcgac tggattaaaa
    33961 ttctcttgcc ggagcgcatc ggctatgtcc attccgacta tatgaccgtc agcgaaaagg
    34021 aagtagcggc taccggcgag ggcggttttt ccctctccat tgacccggag gaaattgctt
    34081 ctctgctgga actgttcaat ggcggcgggg ccagcgccgc cctgtccccg gacgggaacc
    34141 tgtcactgat tgacgacatc ggcagcgccg cgaaaaatgg caagcagttt atcaccgtgg
    34201 aaaccaaaaa cggcaatgtg ttttatctga ttattgaccg ggacgacgag ggaaaagaaa
    34261 ctgtccattt cttaaatcag gtggatgaag ccgatctgct gacgctgatg gaggacgggg
    34321 aaaccgtcac ccccgctgct gtctgctcct gtaccacgaa atgcaaggcc ggggctgtga
    34381 acacgaattg cccggtatgt aaaaccaacc tgaccgagtg cagcggcccg gagccgcagg
    34441 aaccccagcc ggaggaaccc gaagccccgg aggaagaacc caagggcggc gcgggcggcc
    34501 tgatcgtgtt cctgctggtg gcgctggcgg gcggcggtgc ggccctttac tatttcaagt
    34561 tcaaaaagcc caaggctgaa accaccggca gcgacgattt gggcgagtat gattttgggg
    34621 aggacgagga tttggaggac gaggaacccg aaaccgagtt tgacccggaa gctgattttg
    34681 tgagtgaaca ggaggacgag gaatgacata taaacttgta atcgcggaaa aacccagcgt
    34741 ggcccgcagc attgcgggcg tgatcggggc cacggagaaa cacgacggct atttggaggg
    34801 gaacggctat ctggtgagct ggtgcatcgg acatctggtg tcctttgccg acgccggacg
    34861 ctatgacgag cgtttcaaaa aatggcgcta tgaggatttg cccattctcc cggaaacatg
    34921 gcagtacatc gtccccgacg acaagaaaca gcagtttgac gttctctgtt cgctgatgca
    34981 gcgccccgac gtgaccggcc ttgtgtgcgc caccgacgcc gggcgcgagg gggaattgat
    35041 tttccgtttc gtctaccaga tggcgggatg ccaaaagccc tttgagcgcc tttggatttc
    35101 ctctatggag gacagtgcta ttaaggacgg ctttgcccac ctgaaaccgg gcggcgacta
    35161 tgatccgctg tatcaatcgg ccctgtgccg ggccaaggcc gactggctga tcggcattaa
    35221 cgccacccgg cttttttccg tcctgtacca caagaacctg actgtgggcc gcgtccagac
    35281 gccgacctta aaaatgctgg tggacaggga cgccaagatt accgatttca agaaagagaa
    35341 atatcacatc gtccatatca cggcgggcgg cgcggacgcg gtaagcagcc gcttttccga
    35401 cgctgccgag gcaaacaccg tcaaggcggc ttgtgcaaaa gcgcaggccg tttgcgtttc
    35461 cgtgacgcgg gagaaaaaga cggaacagcc gcccaagctc tatgacctta ccaccttgca
    35521 gcgggaggct aaccggcttt tcggctatac cgccaagcag acccttgact atgcccaggc
    35581 actgtatcta aagaagctgc tgacctatcc gagaacggat agtcaatatc ttaccgacga
    35641 tatgctgccc gccgtggaaa gccttgtgtc cggcctgtgg gagctggtgc cgtttgccaa
    35701 gggcctgaac ctttccccgc agtttgaccg catcctgaac agcaaaaagg tgtccgacca
    35761 ccacgccatt atccccaccg ctgaatttgt gaagcagggc tttggcactc tggccgagag
    35821 tgaaaaaaag ctgctgtccc tgatctgctg caagctctta tgtgccgtgg ccgggccgca
    35881 tatctatgag gccgtcaccg ccacatttac ctgtgccggg caggagttta ccgccaaggg
    35941 caagaccgtc ctttcccccg gctggaaaga gattgaacgc cggttccgtt cttccttaaa
    36001 gaccgacgcc gacgaggacg ccgaggcggt gcgggagctg ccggagcttt gcgagggcca
    36061 gacctttgca gacgtggccg cctctgtcac cgagcatttc accacgccgc ccaagcccta
    36121 caccgaagat acgcttttgt ccgctatgga acgggccggg gccgaggata tgccggagga
    36181 tgccgagcgc aagggattag gcacaccggc cacccgcgcc gccattctgg aaaagctggt
    36241 gcagatgggc tttgtccaga gaaaaggcaa gcagctcatt cccaccaagg acggcatcaa
    36301 tctggctgtg gtgctgccgg aggcgcttac ttctccccag cttactgccg aatgggaaaa
    36361 ccggctgacc gagatcgcca aggggcaggc cgatcctgac gaatttatgg acggcatcga
    36421 gacgcaggcg cgggagctgg tgcagaccta ttcctgtatc agtgaggaaa agcagaaatt
    36481 gtttcaggcc gagcgtgttc ctatcgggac gtgtccccgc tgtggtgagg ctgtctatga
    36541 gggcaagaaa aactactact gcggaaaccg ggcctgccag ttcgtcatgt ggaagaatga
    36601 ccgatttttt gaggaacgca agaaagcgtt caccccgaag atcgccgctg cgctgctcaa
    36661 aaccggcaaa gtcaaggtaa agggcctcta ttccgtaaag accggcaaaa cctacgacgg
    36721 gacggtgctt ttggctgata ccgggggcaa gtacgtcaac taccgcattg aacacaggag
    36781 ctaaaggaca gtaacacagc aagcataggg agtgtggcta cactcccggt aaaatccccc
    36841 ggccgcgtcc ctgccgggcc ggggattttt gcttgttggg gaagtcccca accccctatt
    36901 ttttgaaaca gaacagagaa aggagtgacc gcgtgaacga agtattttct atccaagtcc
    36961 ataaccggca gtttttggaa cagggcaaac aaggcgtctg gctggatttg cccaccacaa
    37021 ccgaaacgct gcaagccgcc ctgcgggatg ttggcattac tgccgacaat ccgcaggatt
    37081 tttttatcaa cgggtattcc tgcccggagg acagacacct tgccattccc tatgacatgg
    37141 tgctggccgc cgacgtggac gagctgaatt tccttgccgc ccggctgggc cagcttgacg
    37201 ccgccgagat cgcagagctg aacgccgccc tgcaaaatcc ccgtggcggc tttgagaata
    37261 tcggccagat catcgactac accgaaaatg tggattacta tgtgcatctt cccgacgtta
    37321 ggggggatgg acagttgggc gactactacc tgaaccgttc cggcatggtg gatatgccgg
    37381 aggaatggaa agccgggatt gatacggcgc agtttggacg ccacattgcc cagcaggaac
    37441 aaggcgtttt tacccagtac ggctatctgg tgagaagcgg cgacgaatgg cagcgcgtcc
    37501 atgagggcca gccggtgccg gaggaatacc gggtgatgca ctacccccag ccggaagctg
    37561 accgggagga accgcaggcc cagcagccgg aggcatccga aagccccgac aaagcgccgc
    37621 ccccggttat cccgatcatg ctggacggca tgaacagcaa agaacgcatg aaagtgatta
    37681 ctgaccgtct ggaaaccggc gtacaggagc tttttgaaag tagccgctat atggcttact
    37741 tatccgctat gtccaagatg cacgattaca gtctgaacaa tacgctgctg atcgccatgc
    37801 agggcggttc gattgtggcg ggctataaca ggtggcagga tgaatttgac cgccatgtga
    37861 agcggggcga gaaaggcatt aagatactgg ccccggcccc gttcaaggtt aagcagcaga
    37921 tggagaaaat cgacccggcc acccagcagc ccgtgatcgg cgcggacggc aagcccgtga
    37981 ccgaagaaca ggaagtgacg gttccggcct ttaaggtggt gacagtcttt gacatcagcc
    38041 agaccgaggg caaggatttt cccatgccct atatcagtga acttaccggc gacgtgcagc
    38101 agtatcagga tttctttgcc gcgctggaac ggacatcacc ctatcccata agcattgagc
    38161 cgatttcaga cggggccaag ggccgctgct tctacgcaaa acagcgcatt gccatcaacg
    38221 agggcatgag cgaattgcag accctaaaga ccgccatcca tgagattgcc catgccaagc
    38281 tccacgccat tgaccgggag gcccccgccg aggaacaggc ggcccggcca gaccgcagca
    38341 cccgcgaggt acaggccgaa agcgtggcct ataccgtctg ccagtattac gggctggaca
    38401 cgtcggacta ttcttttggc tatgtggccg attggagcag cggcagggag ttatccgagc
    38461 tgaaagcctc ccttgaaacc atccgggcca cggccagcga attgataacc gagatcgacg
    38521 gccattttgc ccagcttcag caggagcgcg aggcccggca ggaacaggag gccgtcacgg
    38581 ttgacgttac cccggaggca gcggccccgg cgcaggccga ggaagaacag gccccggccc
    38641 ttgatccggc agccgagccg cttgtgactg tgctatggag cgaaagcccc cagctaaagg
    38701 acggccagca aatgccgttg catgaggccg acgcgctgtt tgcccgtctg gatgcagcat
    38761 atccggcaga ggcaggctat gacaaaaccg cgttccgcat tgactgtatc tttcagggcg
    38821 agccggacag ctacgagggg cggcaggatt ttggagatag ggacggttcc ctgatcgaac
    38881 atatccagag ctaccaccaa ttttacgcgc aggacgaaaa ctggaaaaag catgtaatcg
    38941 ctacaaccgg catagagggc tgggagcagg acaaggccca tcgggatatg ctgttaggcg
    39001 agtttgtccc ctatatgaag ctgcattgca gccttgccca aatggaacag acggcgcagg
    39061 gccgcttaca gtccggggaa accctgacgc cggaggaaac ggcctatttg ggggcgcttc
    39121 tctcttatgt gcaggagtgc cgccccatgc tcaatcaggg ccagtatcag ttgccggagc
    39181 cgccgaaact ggctgacttc gaccagagct tgcaggacta caaagcacag gtacaggccg
    39241 agatcgcgca ggaggccgcc gcagccggta tgaccgtgga ggaatacgcg gcggctggct
    39301 atgaagcacc ccaacaggac accttttcca tctaccagct tcgaggcggg gaggaaaccc
    39361 gcgactatcg gtttgagcca tacgagcgtt tacaggccgc agggctggcc gtggacagtg
    39421 ccaactatga ccttgtttat acggcaccgc tggataaaag cacgtctttg gaggatattt
    39481 accgcacatt caacatcgac cacccggcag attttagggg acacagcctt tccgtttccg
    39541 acgtggtggt gctgcaccag aatggacagg acaccgccca ctatgtagac agcttcggct
    39601 atatggaagt gccggagttt ttgcgggaaa atcccctgcg taccgccgaa ctgtctacgg
    39661 agcagaacga aaacatgatt gacggcatta tcaacaatac gccgcccgaa ccgcagccgg
    39721 aggaacccga acagctatct tttgaggatg tggcagttgc ttccggccag acacaggagg
    39781 aacccccgga ggctgcgccc ccgcaaacgg ctgtccgcta ttatcccatc aatgagggag
    39841 ccgcccgccg cgccaaggaa gccatgagct tttctgacta ccggcccggc agcgccacgg
    39901 cggaataccg gcactatgtc gatcaggccg tggaaattgc ggagcgccag aaaaagcgtg
    39961 tggagccaga gtatcacgaa aagatagaca gcctccttga cacctacgcc cgcaagctgg
    40021 ccgagaacat gaataagggc tatgagatta ccgcccgcgt cccctctgtc ctgattgccg
    40081 ggccgtccaa tttcccggta cgggcgaaag aaaagcagaa cgccgccagt gaccgcaaca
    40141 tggaggaatt tcagtacatt cagggcttgc ttgacaaaat ccgcagcacc ggcatgggtg
    40201 gtatcagcgc cgacgatccg caggccgttt ccaagctgga aaagaaactg gaaaagctgg
    40261 aagcgtcgca ggagttgatg aaggccgtca atgcctacta ccgcaagcat gggacgctgg
    40321 acggatgccc ccatctgacc gaaagaggca ttgaaaacct gaaagcggat atggcgtcgg
    40381 gctggcacta tgagaaaaaa ccgttccagt cctggcagct ctccaacaat aacgccgaga
    40441 tacgccggtt aaagggccgc attgaggaat tgacccgcca aaaggaggcg gcctatgtgg
    40501 gatgggagtt tgacggcggc actgtggaga tcaaccgggt ggccaaccgc ctgcaaatct
    40561 tctttgaggg gaagccggat gctgctgtac gcgacgagct gaaaagcaac ggtttccgct
    40621 ggtcgcccaa ggccggggca tggcagcggc agttaaacga caccacaatc cgcgtcgctg
    40681 accgtatcaa gtgcatccag cccctttccg gcgaaaagcc cagcgcctta cagcttgcgg
    40741 cccgcaggga gaaagaaaag ccctctatcc gggcgcagct caacgcggcc aagaccgaac
    40801 aggccaagca gccaccggcg cgggaaaaat caaagggatt ggaggtagag taaatgcaga
    40861 agtttgaaaa tgtggacgtt ctggccgcgc tggaacagat catgcggcag aacaccgcgt
    40921 tttatcaaag tgactttggc attgacaaag acattatcag gcgggatgcc gccagcgatc
    40981 aggccgcaga caagaccctg ctctggatgt cccggccctc cgggacgtac tgtttccggg
    41041 aacgtgatgt tttcctgaaa gacacccgac agtacaacac atggaaattt tacggggagc
    41101 agacccgtga caagatactg gcctacgcgg tggagctgac cggcacacag ggcggcacta
    41161 tccggggcaa tctctatgag ctggactatc cgcagcattt ccggcacgtc attgaaaccg
    41221 cccagccggt gagtgtgaac cggctctttt atgagcatgg cacccgtgac atcccggaga
    41281 gccagcgttt tgacggaagc cctgaccggg tattgggaaa ctttttgtac tatgaggccc
    41341 agccccatga cccggctgtg ctgcaggagg cgctacggac ggagcagcac agccgggggc
    41401 gggccgtccc cggcgatttc aaagtccacg tcaccgcttt gcatgacagc cggattgaaa
    41461 ccgaggcccg gcgtattgtg gacagcctga aagatctgtc cgcgcctaac agccccaata
    41521 aaacgcattt catggtggag atctccccgc actttgcggc gattgcttcc accaaggata
    41581 atgaccggct ctttgccatg ctgccctata aatccctgtg ttttaccggg atgaaaggca
    41641 ggcatggcgt gtatgccgtg attaaccggg acgagcgccg ggatgtgagt attcgcaggc
    41701 cccggccctc tatccgcaag cagctatccg acagcaaaca ggccgccagc ccaaaaaagg
    41761 cggcggccag aaccaaaaag aatgaattgg aggtataagg aatgatgcac tttaccgtgg
    41821 aggaagaaaa cctgatctgt atgtaccaca atgcagaccg gcgcagaacg ataaacaggc
    41881 tcaccgccgt catgccggtt atggatgggg atatgcgggc gcaggcccag cagacccttt
    41941 ccaagctgga acgcatgacg gatgccgact atgacggcca gaagttccac ttcacagacg
    42001 aataacgaac agcagacgcc gggcgcggcg gccatgtggt cgcttgcgtc cggcgtcttt
    42061 tctttttgcc tattcctacg catttagaac ccggtattgt cggggatagg atgaagtatt
    42121 cgcttgcccc tgcaaacatg cgggagaagc cttgcagcgg gcggttttca atcccttaaa
    42181 tgcgtaggtt gcagctataa ttagacccct gaaacagata tagccgcgca gcgggtgtat
    42241 ctgtttcagg ggtgtgcagg tgatagccgc aaagcggcgt aagagggtgc agccctcttg
    42301 ccgtctatct ctgttccagc gggaagctcg gcaatgcttc cagcagtttc agggaaatgc
    42361 gctgccgtat atcttcgtca atgactttct tctttttgcc gtttgcctgt ctgatctcca
    42421 ccgtggaaag ctcgttgatg tagtcagcat aatgggccaa cactttttcc gtggcccacc
    42481 tctcaccggc aacggcggct ttgatcgttt catagtccag cagcttttct tgtccgtcca
    42541 tcctgatacc cccgtcagac tgattttttc tatgatacca tattcggaag catggaacca
    42601 gagaaaacgg cgcttttctg ctggtaaatt ctacgcattt agaacagcac ttcggcgggg
    42661 ataggataaa acacccacat accccgctga ccatgccgga aaagccttga aatataaggg
    42721 cttttacagc tctaagtgcg taggtttcca gtatcttttc cgctggcgct cggcctcttt
    42781 gcggcggcgc atccgtttgg agcaggccgc gcagtatttg gcccggttag acttcggcgt
    42841 gaacgtggcc ccgcactcac agcagcgttt cacttcgtcc cggtctttgg tgatctcggc
    42901 atacagggcc gcatccagcg ggaggacagc ggccttaaac cagcggcaca gaagcgaata
    42961 ggaaatgctc tgtacgcaaa tacattcttc gccgtcgtcc agcaacaggc agttgccgtc
    43021 acaatagttg cagcactcat gcgtcagctt ccgggccttg cgatattgag ggtaattcat
    43081 tcgtggaata ctcatagctc ttgcgtccat tcctttcccg gcgtcatacg caaaatgccg
    43141 tccacattct gcttgatgat gccgtattcc tgcatctgct ttttcgcttc gccgtacttc
    43201 tcatacagcc gttccttttc ctcggccaat cgcttgtact cggctttcag ggcgtacaga
    43261 tcggggagct ttttcacacc ctgtcccttt agggcctttg ccgcagcttc atacaaaatc
    43321 aacgtgcttt catgctcacg ccggaatttt gccttatccg ctgcattgcg gtattccata
    43381 gccacgggcc agagctgctt gtaggtagaa atattcttta tcagcaccgc catatccgca
    43441 agccggtgtt ccatagcttt gagggtagcg gcggcctcgt cgctggcggc ggtgatctcg
    43501 gccactttgc tttccaaatc cgggtaggtg tctatcccat gttcagtcag gaaattcaaa
    43561 gtctttgcgg cctgcttcaa attgtggagc ttcgcccacc tctcatagcc tgccgactgc
    43621 tgggccttaa tgttgttttc cagatcaatc cgcaaagaaa tccccttgcg ctctttgggc
    43681 gctctggtga taacgcggcc tttgatacgc tctttgatgg cttcctcggt gtaggcttcc
    43741 cccaatgtct tgcagcgggt aaagcgttcc tgtccgggag cgcggaaaga aataaacttt
    43801 cccggcttga tctcatagcc ctgcgcctgc atgagccgca ggaaatcatc aaaatctttt
    43861 gcctgcggga tagcggcgtc aatggctgct ttgagcttcg ctttccagct cgtccctttc
    43921 cgctgggcgt cccactcggc atagctcttg cccttactct ggccgggctt caccacggac
    43981 aatccgtttt ccttgcacag cctgtcgctg gtgcgccgga tataggcata gctctgtttg
    44041 ttggaaatgt acttgcgctg atttaccata tccacggcac agaaaataat gtgattgtgg
    44101 acgtggccct tatcaatatg ggtagtcagc acatagggat atttcccttg cagcacttcc
    44161 tcggcaagct gcttcccgat ctcatgggcc tgttccggcg tggcttcgcc gggggcaaag
    44221 gattgtatca ggtggtggcc cagattattg cccttttggt atgcctgcgc cagcagcatt
    44281 tcaaattcaa tgtccgctgt ttcataagag caggcaaagg aggccaccag cagcttgccg
    44341 tcagttttgt ccgggttctc gatgtagtca agggcttttt tcagcgttga cttgatagga
    44401 tgtatctttg taactgccat atctgcgcca gcgccccctt tatatcctcg atgtcctgtg
    44461 cgtacaccgg ccccataccg ttcagcctct ttgcaatctg attgacgttg acgccgatct
    44521 tctgtatctc ggcggtctgc gcccggatgt cgctggtgtc catgttgacg atatagccgt
    44581 caatgagcat cttccgggcg taggctgaaa agttcttcgt atgcagcagc gccattttct
    44641 gttcaatcat agcccgttcc tccggcgtca cagggacgcg cagcacgatt tttcgctttc
    44701 tgtttgccat gcttcaccgt cctttccatt caggggtttg gggatttccc caacaagcaa
    44761 aacggacgcg gcccggcagg gacggggcag gccgtttttc cgggagtggc tacactccct
    44821 atgcttgcta cgttactgtt tctccactaa caagacgttt ccaaagcccc aaactacccg
    44881 caaaatagcc ctctggaaag aaaaaagtgc aaaaaaatag aagctacgct gtgcagctcc
    44941 cgctaaaagt aaaacagagg atgccagtta cacatcctcc gtttcattct tcttcggctt
    45001 ttcaattagt atggcgctgt caatcgcgcc ctcgatgatc ggcaggtatt cttcggggca
    45061 gagtttcagc ttgtggctga ccctttgccg cagttcgctt tcctcccgca tgatctccgg
    45121 gttaaagtag cgttccacag gaagcccgca ggctttaata agctgtatga caaccggcag
    45181 actgggaatg gccccgtcgt tttcgatgtt ggcaagatag cgccagtcaa tatttatttt
    45241 ctctgccatt gctttgcgcg aaacgcgctt tgcttgccgg gccgctttca catccgcgcc
    45301 gaaagtttca aagccgggac aatcttcaat attcgccata tcgcacctcc cacttacatt
    45361 gtatatttca tactctgccc gtggaatgtt ggtatatgca cctttcttaa agtttatatt
    45421 tcacattttg tttcttttat tcttcgagca aaagaattat aatgtaccct gtggaggtgt
    45481 actatggact acatgacgtt gaaagaggca ggcgagaaat ggggtgtgcc cccacgatgg
    45541 ataaactact attgtgctgc gggacgcatc cccggcgctg taaagatggc gacgatctgg
    45601 ctcattccca aaactgcgga aaagccgatt gacaggcgta caaagaaagg aaaggcggtt
    45661 actgaatagt ataatgaaac cggcgcattt tggatatggt tttgaagaga gagaaagcat
    45721 ggtattttgt cacggaaaaa ctataagatg atagatggca ggctgctcca gaccgataaa
    45781 aagtattctc atctgaaaca gaaagaaaag atttacacat ggatgtatga agaagcgaaa
    45841 ctttatcatg acagaaatgg aaaatgcccg aaacaaagac gaggaagctg ctgctgtatc
    45901 agccgtatat ggtagaattg aaaaggccgg catctggatt ctttatggtg aagtctccag
    45961 acattggata actgctggtc taatgcgcct ttgagtatgg ggtgattgat tttaataccg
    46021 gggaggtacg gctttttgag gaaaatattc tgggatttac atttggcgat tattacattt
    46081 cctgtaactg caaggacacg gatgatgaag atatgaggaa tatagacttg ctgattttct
    46141 actgtcttga acggtattag gactgaatct gctgcgcgga gtgcggcaag agcgaccgga
    46201 gggagtacac acaggccctg cctgtgtgaa aagccatacc gcaaggtgca ttcgcaaaaa
    46261 agaggccgcg cattttgctg aggcttgggg tgtccccaac aagcaaaacg agaacgccgc
    46321 aggcgggcaa gtggtggatc agaatatccg taaaggatat tctgatccat tgcttgccag
    46381 tattatcggg atgttttgac aaccgctgta atcgaaacat cccaaacatt aagaaggtgt
    46441 taattattat tatcttgatt taattcgttt aaaccaataa tatagataca tacaccatga
    46501 aaagaagcga gtatcccaat gacggatgat taaaatccga aaagaaatcg ctttgaaact
    46561 acagggaaac tacagactgt tatgttatct tcttaaaatg gagggatttt tatgtcgata
    46621 cgaattctac ttgtcgagga tgatgatcat atctgtaata cagtaagggc gtttctggct
    46681 gaggcaggat atcaggtgga tgcctgcaca gatggaaatg aggcatacac caagttttac
    46741 gaaaacactt atcaactggt tattcttgat attatgctgc ccggtatgaa cgggcatgaa
    46801 cttttgcgtg aatttcgtgc gaaaaatgat actcccattc tgatgatgac agccctgtcg
    46861 gatgacgaaa accaaatccg ggcgtttgat gcagaggcag acgactatgt aacaaagcca
    46921 ttcaagatgc agattttact aaagcgggtg gaagccctgc tgcggcgcag cggtgcgctg
    46981 gcaaaggaaa tccgtgtcgg caggctgaca cttctgccgg aggatttcac ggtactttgt
    47041 gacggtacag agctgcccct gacacgaaaa gaatttgaaa tccttttgct gctggtgcag
    47101 aacaaaggca gaaccttaac gcatgaaatc attttgtcac gtatatgggg atatgacttt
    47161 gagggtgatg gcagcacagt ccacactcat atcaaaaatt tgcgggccaa gctgccggaa
    47221 aatatcatca aaaccattcg cggtgtagga taccgattgg aggaatcata ataatggaaa
    47281 gaaaagggat tttcattaag gttttttcct atacgatcat tgtcctgcta ctggttgtcg
    47341 gtgtaacagc aacactgttt gcacagcaat ttgtgtctta tttcagagtg atggaattac
    47401 agcaaacagt aaaatcctat cagccattgg tggaactgat tcagaatagc gataggcttg
    47461 atattcaaga ggtggcaggg ctgtttcact acaataacca atcctttgag ttttatattg
    47521 aagataaaga gggaagcgta ctctatgcca caccaaatgc caatacatca aatagtttta
    47581 gacccgactt tctttatgtg gtacatagag atgataatat ttcgattgtt gctcaaaaca
    47641 aggcaggtgt gggattgctt tatcaagggc tgacaattcg gggaattgtt atgattgcga
    47701 taatggttgt attcagcctt ttatgcgcgt atatctttgc gcggcaaatg acaacgccga
    47761 tcaaagcctt agcggacagt gcgaataaaa tggcaaacct gaaagatgta ccgccgccgc
    47821 tggagcgaaa ggatgagctt ggcgcactgg ctcatgatat gcattccatg tatgtcaggc
    47881 tgaaagaaac catcgcaagg ctggaggatg aaatcgcaag ggaacatgag ttggaggaaa
    47941 cacagcgata tttctttgcg gcagcttctc atgagctaaa aacgcccatc gcggctacaa
    48001 gcgttctgtt ggagggaatg cttggaaata tcggtgacta caaagatcat tctaagtatc
    48061 tgcgcgaatg catcaaaatg atggataggc agggcaaaat catttccgaa atactggagc
    48121 ttgtcagcct gaatgatggg agaatcgtac ccatagctga accgttggac atagggcgca
    48181 cggttgccga gttgctgccc gattttcaaa ccttggcaga ggcaaacaac cagcggttcg
    48241 tcacagatat tccagccggg caaattgtcc tgtccgatcc gaggctgctc caaaaggcac
    48301 tatccaatgt catattgaat gcagttcaga acacgccgca gggaggcgag gtacggatat
    48361 ggagtgagcc tggtgctgaa aaatgccgcc tttttgtttt gaacatgggc gttcacattg
    48421 atgatactgc gcttccaagg ctgttcaccc cattctatcg cattgatcag gcgcgaagca
    48481 gaaaaagtgg gcgaagcggt ttaggacttg ccatcgtaca aaaaacgctg gatgccatga
    48541 gcctccagta tgcgctggaa aacacctcgg atggcgtttt gttctggctg gatttaccgc
    48601 tcacatcaac attgtaaata tttaaaactt aaatgatttt gaccgacagg tataaccctg
    48661 ccggtctttt tttgctgcta caggaaaact acagactggc tacagggaaa gtacagatgc
    48721 gcttgccata ataacaatcg taccagccac aaatcgtagt tttattgcaa aggaggcatt
    48781 cgatcaaatg gaaaaaagca actatcattc caatgcggat catcacaaac ggcatatgaa
    48841 acaatccgtg gaaaagcggg cttttctatg tgcgttcatt atttcgttca cagtctgcac
    48901 gctgtttttg gggtggagac tggcttccgt attggaggca acacagatac cgcccatccc
    48961 tgcaactcat acaggcagca gcactgacgt agtggagaat ttggaggaaa acgctcttgc
    49021 caccgccaaa gaacagggag atgaacagga atggagcctg attttagtga acaggcagaa
    49081 ccccatcccc gcacagtacg atgtggagct tgagcaacta tcaaatggtg agcggataga
    49141 tattcggatt tctccctatc ttcaagattt gtttgatgcc gcaagaactg atggagttta
    49201 cccgattgtc gcatccggat accgaacaac agaaaaacag caagaaatta tggatgaaaa
    49261 aattgccgaa tataaggcga aaggctacac ctctgcacag gctaaagcgg aagcagaaac
    49321 ttgggtggcc gtgccgggaa cgagcgagca tcagcttggt cttgctgtgg atatcaatgc
    49381 ggacggaatt cattcaacag gcaacgaggt ttatagatgg ctggatgaaa acagctatcg
    49441 ttttggtttt attcgtcgct acccaccaga caagacagag ataaccggtg tgagcaacga
    49501 gccgtggcat taccgatatg ttggcatcga agctgccaca gagatgtaca accaaggggt
    49561 ttgccttgag gaatatttaa aaccagaaaa atgagaaaag gatgttatgt tatggacaga
    49621 aaaagattga cacagcgttt cccgttcctg cttccaatga gacgggcgca gagaaaaatg
    49681 tgcttttatg cgggaatgag atttgacggc tgtcgctatg cgcagacgat aggggaaaaa
    49741 tcgctttccc atttgctctt tgaaacggat tgcgcattat acaaccacaa taccggattt
    49801 gacatgatat accaggaaaa caaggtgttc aacttaaagc tggcagcaaa gaccttaaac
    49861 ggcttattga taagaccggg ggaaaccttt tctttctggt ggctggtacg ccatgcggac
    49921 aaagataccc cctataaaga cggccttacg gtgaccaatg gtaaactcac caccatgtcg
    49981 ggcggcggta tgtgccagat gagcaattta ctattttgga tgttcctgca tacgccattg
    50041 acaattatcc agcgcagagg tcacgaagta aaggagtttc cagagccaaa cagtgacgag
    50101 attaaagggg tggatgcaac cattccagag ggctggattg atttaaaagt gcgaaacgat
    50161 accgactgca cctaccaaat atgggtgacc ttagatgatg agaaaatcat cggtcaggtg
    50221 tccgccgaca aagagcccca agcattatac aaaattacaa atggcagtat ccagtatgtc
    50281 cgtgaaagtg gcgggattta tgaatatgcc caggttaaac ggatgcaagt tgccttaggt
    50341 accggggaaa taatagattg caagctgctt tatacaaaca aatgcaaaat ctgttatccc
    50401 ctcccggaaa gtgtggatat tcaggaggag aaccaatgag aaaaagtgtg ggcattactg
    50461 tttttggatg cgaacaggat gaggcaaatg ctttccgcgc tttatcgccg gattttcata
    50521 ttatccctac gctgattagc gatgcgatat cggcagacaa cgcaaaattg gccgctggca
    50581 atcaatgcgt tagcgtaggc cataagtccg aggtttccga ggcgacaatt cttgcgctga
    50641 gaaaggtcgg ggtaaaatac atttctaccc gcagcatcgg ctgcgatcac attgatacga
    50701 ctgccgccga gagaatggga atctcggttg gcacggttgc gtattcgccg gacagcgttg
    50761 cggattatgc tttgatgctg atgctgatgg ccatacgggg tgcaaaaccc accatgcacg
    50821 ccgtggcgca acaagatttc agattggatc gtatccgggg gaaagaactg ggggatatga
    50881 ctgtgggagt tattggaacc ggccatatcg ggcaagcggt cgtcaaaagg ctgcggggat
    50941 ttggatgcca tgtgctggcc tatgataaca gccgaaaaat ggatgcagat tatgtccagc
    51001 ttgatgagct tctaaaaaac agcgatattg ttacgctcca tgtgccgctt tgtgcggata
    51061 cccgccatct gatcggtcag aagcaaattg gagagatgaa gcaaggcgca tttttaatca
    51121 acaccgcgcg cggagcgctt gtcgataccg gggcgctggt ggaggcgttg gaaagcggaa
    51181 agctgggtgg tgcggcactg gatgtgttgg agggcgagga tcagtttgtt tataccgatt
    51241 gctcgcagaa agtgcttgac catccctttt tgtcgcagct tttaaggatg ccgaatgtga
    51301 tcatcacacc ccatacggcg tactacactg agcgtgtgct gcaggatact acagaaaaaa
    51361 caatcaggaa ttgtctcaat tttgaaagga gtttacagca tgaatagaat aaaagtcgca
    51421 atcatcttcg gcggttgctc ggaggaacat gatgtgtcgg taaaatccgc aatagaaatt
    51481 gctgcgaaca ttgatacgga aaaattcgat ccgcactaca tcggaattac aaaaaacggt
    51541 gtatggaagc tatgcaagaa gccatgtacg gaatgggaag ccgacagtct ccccgccata
    51601 ctctccccgg ataggaaaac gcatgggctg cttgtcatga aagaaagcga atacgaaaca
    51661 cggcgtattg atgtggcttt cccggttttg catggcaaat tcggggagga tggtgcgata
    51721 caggggctgt ttgtattgtc tggtatcccc tatgtgggct gtgatattca aagctccgca
    51781 gcttgcatgg acaaatcact ggcctacatt cttacaaaaa atgcgggcat cgccgttccc
    51841 gaatttcaaa ttattgataa aggtgacaag ccggaggcgg gtgcgcttac ctaccctgtc
    51901 tttgtgaagc cggcacggtc aggttcgtcc tttggcgtaa ccaaagtaaa cggtacggaa
    51961 gaacttaacg ctgcgataga agcggcagga caatatgatg gaaaaatctt aattgagcaa
    52021 gcgatttcgg gctgtgaggt cgggtgtgcg gtcatgggga acgaggatga tttgattgtc
    52081 ggcgaagtgg atcaaatccg gctgagccac ggtatcttcc gcatccatca ggaaaacgag
    52141 ccggaaaaag gctcagaaaa tgcgatgatt acagttcccg cagacattcc ggtcgaggaa
    52201 cgaaatcggg tgcaggaaac ggcaaagaaa gtatatcggg tgcttggatg cagagggctt
    52261 gcccgtgttg atcttttttt gcaggaggat ggcggcatcg ttctaaatga ggtcaatacc
    52321 atgcccggtt ttacatcgta cagccgctac ccacgtatgg tggccgccgc aggaatcacg
    52381 cttcctgcac tgattgacag cctgattaca ttggcgttaa agaggtgata agtatggaaa
    52441 atggtttttt gtttttagat gaaatgttgc atggcgtccg ttgggatgcc aagtacgcca
    52501 catgggataa cttcacagga aaaccggtgg atgggtatga ggtgaatcgc atcatcggca
    52561 caaaggccgt ggcgtttgct ctgcgcgagg cacaaatcca tgcggctgcg cttggctatg
    52621 gcttgctttt atgggatgga tatcggccaa gaactgcggt ggactgcttc ctgcgttggg
    52681 cagcgcaacc ggaggacaat ctcacaaaag aaaaatttta ccccaatata gagcgagccg
    52741 agttgattac aaagggttat gtggcctcac aatccagcca tagccgtgga agcgcaattg
    52801 atcttacgct ctaccacctg gatacagggg aacttgtttc aatgggaagt aacttcgatt
    52861 ttatggacga acggtcgcat catacagcaa aagggatagg ggatgcagag gcacaaaatc
    52921 gaagatgctt gcgtaaaatc atggaaagca gcggatttca gtcttatcgc tttgaatggt
    52981 ggcactataa gttgattgat gagccatacc ccgataccta ttttaatttt gccgtttcat
    53041 aatgaaagta ttggttttgt atcagatttt ggatttgata aaatctatgc tggtcatgga
    53101 caatctttta ctggaaagtg gttaaagcat cttttgtaat acattgcaaa ttatagtatt
    53161 caatctgccc agcggcagaa aagaaaaaaa ccgctgttgg gcagaccttt cccacgccca
    53221 ttttgtattt tgctggcggc ggttttgaga aatcaaggcc gccgttcttt tctggcagca
    53281 aaaggtgtgg cggcccatag gaggatgccg ctatgctgtt ttgctattgc catgctctaa
    53341 tccacttcca ccactcaaaa aa
//
LOCUS       DAMYXM010000014        51220 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.014, whole genome shotgun sequence.
ACCESSION   DAMYXM010000014 DAMYXM010000000
VERSION     DAMYXM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.014"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            330..1175
                     /locus_tag="PNS21_000954"
     CDS             330..1175
                     /locus_tag="PNS21_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDI5158679.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="PNS21_000955"
     CDS             1456..2676
                     /locus_tag="PNS21_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HDI5158680.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="PNS21_000956"
     CDS             2673..5393
                     /locus_tag="PNS21_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI5158681.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="PNS21_000957"
     CDS             5377..6321
                     /locus_tag="PNS21_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDI5158682.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="PNS21_000958"
     CDS             complement(6390..7400)
                     /locus_tag="PNS21_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDI5158683.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="PNS21_000959"
     CDS             complement(7580..7924)
                     /locus_tag="PNS21_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDI5158684.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="PNS21_000960"
     CDS             complement(7917..8351)
                     /locus_tag="PNS21_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDI5158685.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="PNS21_000961"
     CDS             8489..9226
                     /locus_tag="PNS21_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5158686.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="PNS21_000962"
     CDS             9219..10430
                     /locus_tag="PNS21_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5158687.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10634..11416
                     /locus_tag="PNS21_000963"
     CDS             10634..11416
                     /locus_tag="PNS21_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDI5158688.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="PNS21_000964"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="PNS21_000964"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDI5158689.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="PNS21_000965"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="PNS21_000965"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDI5158690.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="tadA"
                     /locus_tag="PNS21_000966"
     CDS             14132..15061
                     /gene="tadA"
                     /locus_tag="PNS21_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDI5158691.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="PNS21_000967"
     CDS             15018..16067
                     /locus_tag="PNS21_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HDI5158692.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="PNS21_000968"
     CDS             16064..16342
                     /locus_tag="PNS21_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDI5158693.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="PNS21_000969"
     CDS             16317..16787
                     /locus_tag="PNS21_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDI5158694.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="PNS21_000970"
     CDS             16804..17106
                     /locus_tag="PNS21_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158695.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="PNS21_000971"
     CDS             17123..17494
                     /locus_tag="PNS21_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HDI5158696.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="PNS21_000972"
     CDS             17466..17825
                     /locus_tag="PNS21_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158697.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="PNS21_000973"
     CDS             17997..19004
                     /locus_tag="PNS21_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI5158698.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="PNS21_000974"
     CDS             19029..20213
                     /locus_tag="PNS21_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDI5158699.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="PNS21_000975"
     CDS             complement(20650..22233)
                     /locus_tag="PNS21_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDI5158700.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="PNS21_000976"
     CDS             complement(22811..22954)
                     /locus_tag="PNS21_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDI5158701.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23177..23656)
                     /locus_tag="PNS21_000977"
     CDS             complement(23177..23656)
                     /locus_tag="PNS21_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDI5158702.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23729..24013)
                     /locus_tag="PNS21_000978"
     CDS             complement(23729..24013)
                     /locus_tag="PNS21_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158703.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24091..24285)
                     /gene="ssrS"
                     /locus_tag="PNS21_000979"
     ncRNA           complement(24091..24285)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PNS21_000979"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24357..25643)
                     /locus_tag="PNS21_000980"
     CDS             complement(24357..25643)
                     /locus_tag="PNS21_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDI5158704.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25781..26452
                     /locus_tag="PNS21_000981"
     CDS             25781..26452
                     /locus_tag="PNS21_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDI5158705.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26418..26906
                     /locus_tag="PNS21_000982"
     CDS             26418..26906
                     /locus_tag="PNS21_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HDI5158706.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26919..27866
                     /gene="prmA"
                     /locus_tag="PNS21_000983"
     CDS             26919..27866
                     /gene="prmA"
                     /locus_tag="PNS21_000983"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDI5158707.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27867..28631
                     /locus_tag="PNS21_000984"
     CDS             27867..28631
                     /locus_tag="PNS21_000984"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDI5158708.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28864..31077
                     /locus_tag="PNS21_000985"
     CDS             28864..31077
                     /locus_tag="PNS21_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDI5158709.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31088..31534
                     /locus_tag="PNS21_000986"
     CDS             31088..31534
                     /locus_tag="PNS21_000986"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDI5158710.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31705..32964
                     /locus_tag="PNS21_000987"
     CDS             31705..32964
                     /locus_tag="PNS21_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HDI5158711.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33053..34000)
                     /locus_tag="PNS21_000988"
     CDS             complement(33053..34000)
                     /locus_tag="PNS21_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDI5158712.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34394..35686
                     /locus_tag="PNS21_000989"
     CDS             34394..35686
                     /locus_tag="PNS21_000989"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDI5158713.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35701..37470
                     /gene="aspS"
                     /locus_tag="PNS21_000990"
     CDS             35701..37470
                     /gene="aspS"
                     /locus_tag="PNS21_000990"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDI5158714.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37579..38454
                     /locus_tag="PNS21_000991"
     CDS             37579..38454
                     /locus_tag="PNS21_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDI5158715.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38720..40603
                     /locus_tag="PNS21_000992"
     CDS             38720..40603
                     /locus_tag="PNS21_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDI5158716.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40678..40962)
                     /locus_tag="PNS21_000993"
     CDS             complement(40678..40962)
                     /locus_tag="PNS21_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDI5158717.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40952..41686)
                     /locus_tag="PNS21_000994"
     CDS             complement(40952..41686)
                     /locus_tag="PNS21_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDI5158718.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41815..42438
                     /locus_tag="PNS21_000995"
     CDS             41815..42438
                     /locus_tag="PNS21_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDI5158719.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42630..43751
                     /locus_tag="PNS21_000996"
     CDS             42630..43751
                     /locus_tag="PNS21_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDI5158720.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43758..46886
                     /locus_tag="PNS21_000997"
     CDS             43758..46886
                     /locus_tag="PNS21_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDI5158721.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46905..50099
                     /locus_tag="PNS21_000998"
     CDS             46905..50099
                     /locus_tag="PNS21_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HDI5158722.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50245..50952)
                     /locus_tag="PNS21_000999"
     CDS             complement(50245..50952)
                     /locus_tag="PNS21_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDI5158723.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16760 a         8948 c        10638 g        14874 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatcccaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggttt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg agactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccatggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttta
    23041 ttttacttga actcattgac tgttttccct ttattttaaa cattataaag accgatgagt
    23101 gcttgccgcg tcttttgttt ttatcacggt tttttgtgca aaaaaacctg ccgatagttg
    23161 gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc tggcaattgg ttttctaaat
    23221 cagttcggac aacaagcaca tcgcaagctg cgtttctaat aacatattca gaaacagaac
    23281 caataaataa acgttctaca gcattcaatc ctgtggctcc taacataatc aaatctactt
    23341 gattatcttc tgggatttct ctggcaataa tttgttttgg tgagccatat tctacgacag
    23401 aggttacatt attcaagcca gctttcttcg cattgctttc ataatctgcc aatgtttgct
    23461 ttgccatttc agtagcttgt tctgcaagca ttccatcaaa ggatgaaacg gtttggaaag
    23521 aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt atttcggata gctacattga
    23581 ccgccttttt aaaagctaat tcagcttcat cagaaccatc tacggcaacc atgatttttt
    23641 tgtattgttg taacataagc attcactcct caagtcaatc gtattgtact cttttattgt
    23701 agaacacttt agggaaaaag agaattattt actgctttgg ttcagcaatt ttttttgcaa
    23761 atcggatact gaaggtggca gaaacaataa ttacgatcag taaaaagacc aaggcaataa
    23821 agcgttgatt gaaaaaagct gttaaaatac ccattacgcc taaaacagca tatgcagctc
    23881 cgtaaaattg taggaatcct tgatttttat catttttgtt gatcaataca aaaaagatat
    23941 cctgtttatt aaataaataa tagctaacga ataataatac taaagtggcg ataatcatta
    24001 aaatcctaat catgaacaaa tcctccaaaa taagcaacaa atagaaaaag caatttgcca
    24061 agagcaaatt gctttattct ttaaaagatg gtaatctccg ttaatgccgt tgtttgtcct
    24121 gtagtattga tcccgcgaat tcaagcaggt gggtcccatg gtcgtagctt cgaactacca
    24181 aatgataagg catgttacca gcagtgacac aagacgggtc ccaagatatc aataaaaatg
    24241 ttcggtcaac acacaaagag gacaattcga acatcacaga tttcccacct ctatagtagc
    24301 atatgaattt aaaaagagca atgagagagc tcgcagaaac agaaccgttt tcttgtctag
    24361 ttttttggag gatgcttttt ccactctttt atacggtaat actgttgacc cagagcctgt
    24421 tcatatttcc ctgtatcttt tggctgataa tactgagcat tcttcagttt atctggtaaa
    24481 tattgctgat tgacccaagc ctgatcaaaa tgatgaggat actgataacc cacgccacgg
    24541 ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat gatcaggaac atctccagct
    24601 ttgccctctc gaatatcagc aagtgctgca tctaatgcca tgtaggcaga attggatttt
    24661 ggcgacaagc aaagatcgac gacgacgtct gctagtggga tacgtgcctc aggcaatccc
    24721 aatttttcag cagctaatac agcgttcact gtacgagccg cagcagcagg attacctaaa
    24781 cctatatctt cataaccgat aaccatcaga cgccggcaaa tacttgccag atcacctgcc
    24841 tcaactaaac gggcgagata atgaagtgca gcatccacat cacttccacg aatcgatttt
    24901 tgaaaagccg aaataacgtc ataatgagca tctccatttt tgtcatgggt aagagctttt
    24961 ctttggacac attcttcaat gatcgacagt gtaagatgaa tccttccttc tttatctgaa
    25021 ggagtcgata gtgtagccag ttccagccca ttcaatgcac tccgcaaatc gccgttcgtt
    25081 gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga ttgcttgttg tccaaggccg
    25141 cgttctttat cccttaaagc atgttcgact gccaactgga tgtcttgttc tgttaatggt
    25201 ttgacttcaa agatctgtgt acggctgcgg atcgctggat tgatggtaat ataaggattc
    25261 tcagtagtcg ctccgatcaa gatgattcgg ccactttcta aatgaggaag cagaaaatcc
    25321 tgttttgttt tatctaatcg gtgtacttca tccaataaga ggatcactgt accactcatt
    25381 tttgcttctt ccgctacaac ttgtagatcc ttttttgaat ctgtagcggc gtttagcatg
    25441 cgaaatgcat aattagtaga gccggcgatc gcacttgcga tactcgtttt tccagtacca
    25501 ggaggtccat atagaatcat ggaagagagc atgcgagcat cgaccatgcg gcgtatgatt
    25561 tttccttccc cgactagatg ctgctgacca acgacttcat cgatcgttcg aggtctcatg
    25621 cgataagcca atggctgttg cataggattt tcctccttct tgtttttacg tcccttaatt
    25681 atatcatatt tagcaagatg cctagaaaga acagccttct gttgttttgt tttcatagct
    25741 tatccaacta atattgtgta tgataaagta ggtgagaaaa atgacggata aacaaaagat
    25801 agatgaattt ttttggaata aaacgacgcc agaagtggca cgggatttat tggggatgta
    25861 tttggagcat gacacaccag aaggaagatt agctggatat attgtagacg cggaagcata
    25921 cctaggtcca gaagacgagg ccgcccatag ctatggttta agaagaacac cgcgtgtgcg
    25981 ggcaatgtat gaaaaaccag gtacgatcta cctttacacg atgcatactc atcggatttt
    26041 gaacatcatt actcagccag aagggattcc tcaaggagta atgatacgtg caatcgaacc
    26101 cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga aaaactggtc cagatatcag
    26161 caacggtccg ggaaaattag tggaagcatt agcaattcct caagaacttt atggtcagtc
    26221 tatagcagat agttcgttac gcttagtttt tgagaaaaaa aagacaccaa aaaagatcaa
    26281 tgctttgcct agaatcggta tcccaaacaa aggtgtatgg acagaaaagc cgttgcgttt
    26341 tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa aaccaaatcg agaaagattg
    26401 gggttggagg aaagaaaatg aaaaagaagg atctattaac atatttagat gaaataattg
    26461 aaaaacaagt gacagactat gatgtagctt tagattggga tacaaaaaat catacgatcg
    26521 agatcgttgt tcgcttgttt gcagaaaata aagcacaatt tgagattgat gatgcagaag
    26581 ggatcgtttc gcaagaacca attatcgaat ttgaagacgg cgtacttctt tttaatcctc
    26641 agaaatctgt ttttgatgaa cgcaattatc tagcagtaat cccttatgaa ggaaaaaaag
    26701 ggttggcaaa atcagtagct gattctttag tggaatatct aaatgaagta ttagctcaag
    26761 ggcaaagcga tttgcttgat tttttggatg aagacgatga cgaagctgta ttcgagcttc
    26821 actgggataa tcagcaacta gcagaattag tagaagtaaa acagcaaaat gaagcagata
    26881 cttatttacc gtacccaagt tattaaggag tgacagatat gaaatggaat gaagtaaaag
    26941 tagaaactga aagtgaagca gtcgaagcag ttgctaatat tttgatggag gcaggggcga
    27001 gtggtgtcgc gattgaagat tctttagatg ttgaacattt tcagtcggat caatatggtg
    27061 agattttaga taaaaatgat tttacatcgc tgaaagaagg cgcactagtc atggcttatt
    27121 ttccggaaac gactttctta ccggaaatct tgccttttat caaagatcgt atcacacaat
    27181 tacctgagtt tggtctagct attggaaaaa ataaagtaac agttagtgaa gtagaggaaa
    27241 gcaactgggc aacagcctgg aagaaatact atcatccggt gcgtattacg cgaatgctga
    27301 cggttgcgcc aagctgggaa aactaccaga cagaagatcc tctagaaaaa attatcacac
    27361 tagatcctgg tatggcattt ggcacaggca ctcatcccac gacttccttg acattgacag
    27421 cactagaaac agtcttgcgt ggtggtgaaa cattgctaga tgtgggaact gggtcgggcg
    27481 tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt ctatgccttt gatttagacg
    27541 aagtagccgt tcgttcggcg aaagaaaata tggatatgaa tgaaatagcc aaagatgtcc
    27601 atgtatcagc taacgatctt ttgacaggaa tcgagattga aagtgatgta atcgttgcga
    27661 acattttagc agatatcatc ttattgatga tcccagatgc ttggcgttta ctgaagcaaa
    27721 caggcacgct gatcgtttca ggaatcattg aagagaagaa gcaaatggtt ctggatgcca
    27781 tgttagaaca agggtttatc gtggatcaaa ttttccagca aaaagactgg tatgcaatta
    27841 ttttgaaaaa accagaggtg gaatagatgc agcggtattt tttaaaagaa tcatatgaat
    27901 caaaagacta ttttgtttta gaaggggaaa actatcacca cattgtacgg gttatgagga
    27961 tggaacccga ggatgaagtt tttttggcat tcaatgatca aatagccatt gttgcagaaa
    28021 tcacggagat tactgaagag gctgtttatc tgaaagaaat taaaaaagaa gaaaatcaaa
    28081 aggaactgcc aagttcgatt accatagcga gtggctatcc taaaggggaa aagctggatt
    28141 ggatcgttca gaaatccact gaattaggag cgcaagcttt tcttggtttc ccggcatcag
    28201 cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg acaacaacgg cttgaaaaga
    28261 ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt gccatcagta gagttattgg
    28321 aaacgaaaca agatttattt gagcggctag atgattatga cgtggtcttg gttgcttatg
    28381 aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa aattcttagt gaaacaaaac
    28441 cagatagtcg aatcttagct gtctttggac cagaaggtgg attatcccgc gatgaagtcg
    28501 attattttgt agaacacaaa gggatacttt gtggattagg accaagaatc cttcgtacag
    28561 aaacagcacc tctttattta ttaagtgctg ctagctatca atgggaatta atgaatcctt
    28621 ctttcagcta acgttcttca ttaataaaag agtatcgaca gaaagaattg aaaaaatagt
    28681 caacaatttg tataatagaa acaattattt atacaatgga tacgaaaaat cgtttgaaaa
    28741 agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa ctgcacggat tttgggacaa
    28801 ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa aaaagaaagg gggatcgatt
    28861 cctgtgacaa aagaagaaat tttaacggga cccggtgtca taaagctcgt tagtcaatac
    28921 atgggtcccg aacatgtagc atttgttgag aaagcatgcg aatacgcgac tgctgcacat
    28981 gacggacagt ttagaaaatc aggcgaaccc tacattattc atcctatcca agtcgcaggt
    29041 atattagcag atttaaaaat ggatccccat acagtggcta caggcttctt acatgatgtt
    29101 gttgaagata cagaaatcac tttagaagat ctgagagaag aatttggcga tgacgttgct
    29161 atgttagtgg acggcgtaac caaattaggg aaaataaaat ataaatccca cgaagagcag
    29221 ctagcagaaa accaccgaaa gatgctgcta gcaatggctc aagatttacg agttatcatg
    29281 gtcaaattag ctgatagact acataacatg cgtacgttga agcacctgcg agaagataag
    29341 caaagaagga tcgctcagga aactttagaa atctatgcgc ctcttgcaca tcgtctaggg
    29401 atcagccgga tcaaatggga attagaagat acagcacttc gttatctaaa tccaaaacag
    29461 tattaccgta tcgtccactt gatgcagacg aagagagaag aacgtgaaaa atacgtaagt
    29521 ggtactgttg aagatattcg aatagcgaca gaagagctgg ggatttttgc agaaatctat
    29581 ggacggccaa aacacattta ttcaatctat cgtaaaatga aagatcagaa aaagcagttc
    29641 aacgaaattt atgacttgct agctattcga gtgatcgtag attcaatcaa ggattgttat
    29701 gctgtattag gagcaatcca cacaaaatgg aaaccaatgc ccggcagatt taaggattat
    29761 attgccatgc caaaagctaa tatgtaccaa tctttgcata ctaccgtgat cggtccggca
    29821 ggaaatccgg tagaaattca aatccgaaca caggaaatgc atgaaatcgc tgaattcggg
    29881 gttgctgcac actgggccta taaagaagga aaaaatgaaa aagtagaacc agatggtatg
    29941 acgaaacaat taagctggtt ccatgagata ctcgaacttc aagacgaaag ctatgatgct
    30001 tctgaattta tggaaggcgt aaaaggagat atctttagtg ataaagtcta cgtcttcaca
    30061 ccaaaaggag acgttactga gttaccaaaa ggatccggac cattggactt tgcatacagt
    30121 atccatacag atatcggtaa caaaaccact ggtgcaaaag taaatggcaa aatggtgcag
    30181 cttgattaca aattgaaaaa cggagatatc attgagatca tgacttctcc aaattcattt
    30241 ggcccaagtc gcgactggtt gaaattagtt gctactagca aagcaagaaa taagatcaaa
    30301 cgtttcttca aagcccaaga tcgagaagaa aatgtgatca aaggccacga atccgtggtc
    30361 aaatgtatta cagatctagg atttacgcct aaagatattt tgacgaagaa caaactgcaa
    30421 gaagcactcg atcgttttaa ttatcaaaca gaagatgatc tctatgcagc tgtagggtat
    30481 ggagaagtta gccccttgac gatggccaat cgtctgactg aaaaagaacg taaagaacaa
    30541 aaaatcgagc agcaaaagca agaagcagaa gaaatcatga atcagccgaa aaaagaacct
    30601 gacaaaatga aagtacgtca tgaaggtggc gttgtcattc aaggggtaga aaacttacta
    30661 attcgtatca gtcgctgctg taatccgatt cctggtgatg atatcgttgg ttatatcact
    30721 aaaggcagag ggatatccat tcatcgtcga gattgtccga atgttcagcc tgacaaacca
    30781 aatgtagcag aacgtttgat tgaagtcgaa tgggaagata catcgaatac acgaaaagag
    30841 tatgatgcag atttggaaat ttacggctat aatcgttcag gcttattgaa tgatgtactt
    30901 caaacagtca atgcgctaac gaaaaatctc aacagcgttg aagcacggac gaataaagat
    30961 aaaatggcga cgatccattt gacggttggt atccagaatt tatcccacct aaagagtatc
    31021 gtggataaaa tcaaagcagt acctgatgtc tacagtgtac gccggacgaa tggataggag
    31081 gacagatatg agagcagttg ttcaacgggt aagtaaagct gctgtgtcag tcgatcagca
    31141 agaaattgga aaaattggct acggcctact tgttttatta ggcattcatg aaaccgatac
    31201 gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa atgcgtattt ttgaagatga
    31261 acaaggaaaa atgaatatta gtgtagaaga tgtgggagga caaattttaa gtatctctca
    31321 attcacactg tttgcggata cgaaaaaagg aaatcgacca agctttgtgg aagccgcacg
    31381 accagaaaca gctattcctt tatacgaatc attcaatgaa ggaatcagaa gtcgcggcat
    31441 cacagtcgct accggacaat tcggagcaga tatgtcggtt tctctcgtca atgatggacc
    31501 tgtaacgatc attattgatt ctaaaaacaa ataaagtaat ctggttttca ctagtaaaaa
    31561 ctatgaaaca cgtcgcttaa gttagactga taaactctaa cttaagtggc ttttttgtct
    31621 gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt aagcttaaat tatacaagtt
    31681 attttttata ggaggttgct gaagatgaaa gaagtaccag tagtagatat caaaatcaca
    31741 gatttaaaaa aattgtatca aaaaggatac aacatgttgg aggagttacg ccatgaagcg
    31801 gacgctcccg tcgtcaaagc aaagattttc aataaagaag ctatcacgat ctacggatca
    31861 tcagcagcaa aagtatttta tgatccaagg aatttcaaac gaaaaggtgc tatgccaaaa
    31921 ttagtattga agacactttt tggtcaaggt ggagtacaga ctttggatgg agctgctcat
    31981 caccatcgga aaaatatttt tatggatcta atgacaccgg aaagaatgga agattatcat
    32041 cgtatcttag ataagaatct gacacaagca cttgaagcac agcatggtca atttgaacta
    32101 tttgatttaa gtaagatggt tttctttaca tctatttgcg agtgggcagg tatcaattta
    32161 tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat atcaaatctc tatgatcagc
    32221 ggaacgttta cttctccgat tgatcacata aaaggtgtcg aaaatcgcaa aaaatcagaa
    32281 aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc ctgtggctgg aaaagaaaat
    32341 gtggcattat atgcttttgc caatgccaca gatttagatg gtcagctttt acctctggaa
    32401 gtggcagctg ttgaattatt gaacatcatc cgtccaacgg ttgctctaac tgtgtgggcg
    32461 gcattgatgg gacatgcact tttcagtaga ccagatttgt atcagcaatt aaaaaatgac
    32521 ttttcgacgt tacaagatcc gtttattcaa gaaatgagac gctattatcc tttcttccca
    32581 atgctacctg cgatttcgct gaaagaagta gaagtagatg gttatcgtat accagaaggc
    32641 agttgggtta ttttagattt atatggaaca gaccatgatg aacgaacagt tgaagcgcca
    32701 gattccttta tgatcaaacg gtatgtaggg aaagcaaaag atatttccta taaagaagaa
    32761 tatgagatga ttgcacaagg cgggggaaat tttcgacaaa tgcatcgctg tgcaggagaa
    32821 tggatcactc ttcatagtct acgtgtgttt tccgatcagc tagtaaataa gtttgagttc
    32881 tctgtacctg aacaagattg gacgattcca ttcaatcagt ttccgactta tccgaatagc
    32941 cgagcgctac tttacaagaa ttagattagc ataaacttat cgaaaaaaag gaagatcaaa
    33001 cagtgctgat cttccttttt tcatattact ataaattgat aaggtcaatt gatcaaaatc
    33061 cacgaaatgc ttcatcaatg gtttgataat cttcattgga taaatgcaaa tctaaagctt
    33121 ggacattgtc agatacttgc tctggacgct tagctccagg tataactaca gacactcgtg
    33181 gattcttcat gtaccaagct aaaacaagtt gcgcaggagt ggcttggtat cttttagcaa
    33241 tggggcgaag tacatcgaca gcggttaaaa tttctctgaa acgttctcct tggaaatctg
    33301 gatttctttt tcgaggatct ccttcaccaa attgtttttc ctcaccaagt tcatattttc
    33361 ctgtcagcaa accggaagca agaggaaaat agggaacaaa actgattttg tttttttcaa
    33421 gataagggaa taattctttt tcagcctgcc gatggatcaa actgtattta tcttctacta
    33481 catcgacata tccatctgca ttcgcttctt tcaattgttc taacgtgaag tttgacacac
    33541 cgactgctcg gatttttcct gcttctttta attcgtgcaa tgtagcaact gattcatttt
    33601 ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc gatgtaatca gtttgcagac
    33661 gttttagtgc atcttccacc gattgtttga gaaactcagg agaattatca aatgttcgcc
    33721 ctttatttgg tacatgagca gcttttgtag cgatgacgac acgtgagcga tcatattctt
    33781 tttccttcag tacctcgcca atcagttctt cggaacggcc gtttccgtaa gcataagcgg
    33841 tatcgatcaa attgatcccg ctattcaaag ctgtacgaac tacttgtttt cctgtctcat
    33901 cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt tgctcctaat ccaagctgtt
    33961 ctgcgtagac ttgtgaatgt ccgattctta tttctgccat agctgattcc tcctttttgt
    34021 ctagtctaaa acaaaatacc aaaaagctga actaaaacgt ttttgtgagg ggaaatagtc
    34081 agatatctct tgactttgag actggttttt agtaggttat aagtaatatc gaatgtcaat
    34141 gaaagagcga gtaagttttt gaaactttac aagagaggat catcgaggct gaaaatgatc
    34201 taaagtttca ttagcctaaa gacactctgg agatggacga gtgaaaagcc gtcccgcgta
    34261 cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat taggtggtac cgtgtatgaa
    34321 tttcattcac ccttgttcat gatgaacaag ggtgattttt ttatttacat agctaagaaa
    34381 gggagagaaa actatgagtt tccaaagacc aaaaggaaca aatgatattt tgccaggcga
    34441 atcagaaaaa tggcagtttg tcgaagaaac agcacgttta ttattcagag attatcagta
    34501 taatgaaatg cgtacaccga tttttgaaca ctatgaagtg atatcccgaa gtgtagggga
    34561 tacaacggat atcgtttcaa aagagatgta tgatttttat gacaaagggg aacgccatgt
    34621 gacgcttcga ccagaaggta cagcaccaat cgttcgttct tttgttgagc ataaattgtt
    34681 cggcccagag tatgcgaaac cgttcaaaac tttttatatg ggaccaatgt ttcgctacga
    34741 acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc ggagtagaag catttggatc
    34801 agaaaaccct gcaactgatg tggagagcat ggtgatggca cttgatttct tcaaacagtt
    34861 ggggatcacg caaatccggc tagtcatcaa ttcattaggt gataaagaaa cgcgtcaagc
    34921 ctatcgtcaa gcattgatcg actatctttt accttttgaa tcagagctga gtgaagactc
    34981 taaacgccgc cttcatgaaa atccattacg cgtattggac agtaaagaca aacgagatca
    35041 aaaatttgtg gcagaagctc catctatctt agattatcta agcgagcctg ctcaaaaaca
    35101 ttttgatacc gttgtttcca tgctggatgc attggctgtt ccttatgaaa tcgacagtaa
    35161 tatggttcgc ggactagatt attacacaca tacgattttt gaaatcatga gtgacgcacc
    35221 aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt tatgacaatc tagtggaaga
    35281 actaggtggt ccttcaacac ctggcttcgg atttgcaatg ggtatcgagc gattactctt
    35341 gactatggag gcagaggaag tagttatccc tgcatttaat gagttggatg cttacgtggt
    35401 cgcactcgga gacgaaacaa atatcgaagc attgaaagtg gtccaagcga ttcgaaactt
    35461 tggtttttca gctgaccgcg acttcatgaa ccgtaaagca aaagcgcaat ttaagacagc
    35521 agataaagcg aatgccaaat tagtattggt gatcggtgaa gatgagcttg ctagtcaaac
    35581 gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt tttccattag aagagatata
    35641 tgaacatttt gatgaagtct atgatgaaat gactttattc gagtaggagg aaaaaaataa
    35701 atggcacaaa gaacgatcta ttgcggatta gtttccgaac aacaattaga acaaacagtt
    35761 acattaaaag gatgggtaca aaaaagacga gaccttgggg gagtaatctt tatcgaccta
    35821 cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg aaatcaacaa agaagcatgg
    35881 gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag tgactgggga agttagaaga
    35941 cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg aattcgaagt gattgcaagt
    36001 gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc aaattgaaga tgatcaagca
    36061 atcagtgacg aaatacgttt gaaatatcgt tatttggatc tgcgtcgtcc aaaaatgact
    36121 aaaaacatca aattaagaaa tgcgacaaca aaagcgattc gtcattattt agatgataat
    36181 gactttctcg atatcgaaac accttaccta gggaaatcta caccagaagg cgcaagagac
    36241 tacctagttc cttcacgtgt ccatgctgga catttctatg cgttgccgca atctcctcaa
    36301 ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt attatcaaat cgttcgttgt
    36361 ttccgcgatg aagatttacg tggagaccgt cagcctgaat ttacgcaagt agatattgaa
    36421 acaacgttct taacagcaga agaaatccaa acttacacag agggattgat tgcgaaggta
    36481 atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc ctcgtatgac ctatgatgaa
    36541 gcaatggctc gctacggcag cgacaaaccg gatacacgtt ttgatatgga attgatcgat
    36601 ctaagcgata cagtcaaaga agtagaattc aaagtcttcc aaatggctct tgaaaacggc
    36661 ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc gctactcacg aaaagacatg
    36721 gatcagttag gtcaatatgt aggacaattc ggagctaagg ggcttgcatg gctgaaagta
    36781 gaagaagacg gattgaaagg tccaattgct aaattcatgg gagaagcaac agaagcaatc
    36841 attaaagcaa cagatgcaaa accaggtgac ttgctgatgt ttggcgcaga taaaagtgaa
    36901 atcgtcgctg ctgcattagg tgctatccgg acacgtttag gaaaagaatt aggactaatt
    36961 gatgaatcga aattcaattt cttatgggta actgattggc cacaatttga attttcagaa
    37021 gaagaaggtc gatatgtctc tgctcaccat ccattcacga tgccaaaaga agaagatatc
    37081 ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag cttatgatat cgtcttgaac
    37141 ggttatgaac taggcggagg ttctatccgt attcatactc gtgagcttca agaaaaagta
    37201 ctagaaactc taggctttac aaaagaacaa atggaagaac aatttggttt cttgttaaca
    37261 gctttagatt acggattccc tccacatggc ggtattgcat taggattgga ccgttttgca
    37321 atgttattag ccggagaaga taatattcgt gaagtcattg cctttcctaa aaacggaaaa
    37381 gcagcagatg tgatgagtga tgcaccaagt atggtttcgc ctttacaatt gttcgaattg
    37441 aatattgatg taacgaatat agaagaatag ccgataaaaa ggtaaaaagg atttgtcgaa
    37501 tacgcttctt ttgacaacct tgatttaatt gaaaaacact ttataatgaa gccataacat
    37561 ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt gggcttatca tgattacacg
    37621 accaagcttt cggtatctat cgtttatgcg ttgatcgcat cagtcgcatt gaatttcttt
    37681 tatcagccag gacatattta ttcaagtggt attactggtc tagctcagat tttgacgact
    37741 ttgtcaaaat cactgatagg attcgaagta ccggtatcaa caactttgta tttactgaat
    37801 atacctttgt ttgtattggc gtggttaaaa atcgggaaga agtttacaat tttcactttt
    37861 atcacagtca ctttgacctc cctttgtatc catattttac cggaaacagt cctatctacc
    37921 gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg gactaggtat tgggctagcg
    37981 ttgagaaacg gcctatcatc gggcggtcta gatatcgtga gtatcacgat tcgtaaaaaa
    38041 acaggaaagt cgattggttc tatttcgatc tacttcaatg cattgatcgt ttttgttgcc
    38101 ggaattctat ttggctggca atatatgttt tacagtacgt tgtctatttt cgtcagtgga
    38161 aaagtaactg atgtcgttta tactaaacaa aagaagatgc aggtaatgat cgtaacgaaa
    38221 catccagaaa aagtagtaaa tgaaatccaa aactgtatgc gtagagggat cacgatcatc
    38281 aatgaagcag aaggtgctta taagcatgat aagcagacag tattgatgac gatcgttaca
    38341 cgatacgaac tgcctctact tagagaagca atgaaagctt ctgatccaca agcttttgta
    38401 agtattgcag aaaacgtcca gatacttggt cgtttctatg aggatggcat ttagatcgag
    38461 tttgacaaaa attcatgaaa tactgctgta gacagacgtt cgtttgtctg cagttttttt
    38521 ctaccatagt agaatcagca aagaaaaact gcttttttat ttgaaatttt cttaaagttg
    38581 aaaaaaagca tgtttatttt tttaaattta tgggatgata gaaaaaaata gtagagaaag
    38641 gacacgattc tacttgctat tttcacttaa tacccgtata ttacgaatga aaaaagttta
    38701 tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta aaaaacagcc gctacatcac
    38761 tgggtttgat gggctccgtt cattagctgt ggtgggtgtt atcttctatc accttctgcc
    38821 aactagtatg aaaggcggct atttgggcgt tccgattttt ttcgtcgtat ctggttattt
    38881 gatcacggat ctgctgcggc aggaatggga acaaaatgga aaaatcaata tttggcaatt
    38941 ttatgtcaga agaatgaaga gactctatcc ggcacttgtt tttatgcttg tgacagcttc
    39001 ggcttatatc actttgtttc agcgagggct gttaaacaat cttcgaggag ttgtggtcag
    39061 cagcttactg tatgtcaaca actggtggca aatcaataac ggtttatctt atttcgatcg
    39121 atttgctaat gagtcaccgt ttacgcatat ctggtcttta gcagtagaag gtcaaaacta
    39181 tttggtttgg ccgatcttgt ttgtattgtt gatggtattt gttcgtaaaa gaaaatggat
    39241 cttttatact gtgttaggta catccttcct ctccgccatc ttgatgatga tcttgtatac
    39301 accaggaggc gatccaaccc gtgtttatta cggtaccgac actcgtctgt tttctatatg
    39361 gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt ttaaagaaaa acatcccgaa
    39421 acaagcgaaa aatattctga atatcgctgg aggaatctcg cttttggcac tgattctttg
    39481 cttcttcttt ttagatgatc attacaattt catctattac ggaggaatgc tcctagtgag
    39541 tctgttatgc gtagtgctag ttgcagtgac agcacacccc ggagcaagtt tgaatcgatg
    39601 gctgacaaat ccagtattta cttggattgg caaacgaagc tatggcattt atctgtatca
    39661 gtttccagtt atgatttttt atgaagcaaa ggtgactgat attgccgatc atgttttgtt
    39721 gcatacttta gtggaaatca cattgattct aatcatcagt gaactttctt atcgatatgt
    39781 ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc caagtgatga aaggatggtt
    39841 cagcaaaccg atcatcagta agagcaaacc atgggttgtt ccaggcacat tacttgtagc
    39901 aattgcactc gttggtttcg ttacggcacc gaaaaacgct gtaacagccg atcaaaaaca
    39961 actgcaggca aaaatcgctg aaagtaaaaa attagcagaa gaaaccagaa acaattcaga
    40021 aaacaccact agttcggttg accaagcgat ccttgataag tacgagttgt ccaaggcaca
    40081 agggaataag gcacaaaaat tagaactaac ggcatttggt gattcagtta tgttggatgc
    40141 tgcaacggat ctacaagaac tttatccaaa agtagtggta gatggagatg tcggacgaca
    40201 attatatgcc agtgtaccgt acattgaaaa attaaaggaa caagatttgt tgaaagacac
    40261 tgttctgatc ggactaggaa cgaatggctc attctcggaa gctcagtttg atagtgtgat
    40321 ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc cgagtaccga cgaagcgctg
    40381 gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa aaatacgaca atatgacgct
    40441 gattgattgg tacagctaca gtaatgacca tagtgactgg ttttatgatg atcaagtcca
    40501 tccaaatcct gaagggatga cacattacat tcacttagtc agcgaagcct tgctaggagg
    40561 aagttcaagt acgaacagca gtgagacaaa cgtgacagaa tgatagctac aaaaaacttc
    40621 aggcaaaaac atttgttttt gcctgaagtt tttttatatt taccgtgttt ttaacattca
    40681 tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa taacgttctt tttgttttcg
    40741 ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg cttcttgaag aaaatacttc
    40801 tgcatggatc atcttaacag gtcttctttt ttgaagacga gtatatttag caccagtacc
    40861 attgttatgt tcctctaatc gtctagaaag atcggttgta tatccaccat aaaatgtctg
    40921 atctttacag aataatacgt aaaaataatg ttcatttgcc atagagcatc tcacgaatct
    40981 ctgcagtgta ttctccatcc atattgtaag tgatcaatgg tggagcgact ttgaatccgt
    41041 ctaatttgcc ttctttgatt ccttcaacta acaaaatatt tgcctctttt ccaggttttg
    41101 ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc ttccatgacc tgaatgattt
    41161 ctaagaaccg atcaggacga tggaccatag taaagcgtcc atttgttttt agtagcttac
    41221 ttgaaatatc gatcacttct tctaacgtcg tatgaatctc atgacgggcg attgccaggt
    41281 gtttattagg gttcgtttta tttgttggca gccctttgaa ataaggtgga ttacagacga
    41341 ctaagtcaca agaattatgt cgaacgacag ataagctgtc tttcagatcg atggtgtgga
    41401 cagtcatttg ttcatttaaa tggttaagcg cgatactacg tcttgccatg tctgctagac
    41461 gaggttgaag ttcgatagcg tcgatagccg cctgggttcg tttacttaaa aaaagtccaa
    41521 ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt tttaggtaca cgggaaaagt
    41581 gagcaagcag cacagcatct aaagaaaaag aaaacacttt ggaactttgg ataatcttga
    41641 tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg aagcatggat tccctccaaa
    41701 atgaaaaaaa tagtcgtctg tattatacta tttttattaa aaagaaccaa ggtggcttgt
    41761 caaacctcta ttttttatgc atactatgac cataagaagg gaaaggaaga gactatgttt
    41821 tatcgattta tgcgcggggt agttcgagta ctattagcct tagttaatgg aaatgcccgc
    41881 tatgagaata aagaagtttt accgcaagat gagaattaca tactagttgc tccccatcgt
    41941 acttggtggg acccattata tctggcggta gctgcagcac ccaaggaatt cagctttatg
    42001 gcaaaagaag aactgttcaa aaatccagtt ctgcgattta tattaactcg atccaatgct
    42061 tttccagtta aaagagaaaa tccaggacca agcgtcatta aaacacctgt aaagattttg
    42121 aaatcgacaa atttgagttt gatcatgttt cccagtggga caagacatgc tacagaactt
    42181 aaaggcggaa tggcattgat ttccagaatg tctaaagcga aaatcgtgcc agctgtatat
    42241 caaggtccgt tgacacttgg ggatctgttc aagcgtcaga gagtgacagt gcgtttcggc
    42301 gaaccaatcg acttatcaga tatcaagaaa acggataaag aaggtatggc ggagatcgaa
    42361 cgacgaacac aagcagcttt tgacaaattg gacaaagaag tgaaccctga tttcaaatat
    42421 gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc ggtcttttga atctactgtc
    42481 ctgttcagtt cagacttttt caatgaaaac gagggagaga cgtcaagtct gccctctttt
    42541 tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa tggatcaaag tgttataata
    42601 attagaagga cattttttga ggtgattgaa tgcgtttttt acatacagcc gattggcata
    42661 tcggaaaaaa actacaaggt tatgacttga ttgaagacca gcgtcatgta ctatcttcta
    42721 tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt gattgccgga gacttatatg
    42781 ataggagtgt cccagctgtt gatgccgtag aattatacaa tgaattgatg gtcgattgga
    42841 atttaaagga aaagatcccg atttttgcta tatcaggaaa tcacgatagc agtacgagac
    42901 tttcagcggg tacgccatgg ttcagttatt cgaactattt tttgcgaaca cagctagcag
    42961 aagcatttga accagtcgag tttaaaaacg tacaattttt ctttcttcct tactttgaac
    43021 caattgcagc gagattatat tttgaagatg acaatatccg aacgatcaag caagcggtag
    43081 ccaaggtgat agataaaatg caggagctat ttgattcaga aaaaaaacaa gtattgatta
    43141 gccatttttt tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc gaagtaggcg
    43201 ggttggattc gatccctggt gaactactta caccgtttga ttatgtagca ctaggacatt
    43261 tgcataatca agctgctttg agacaggata atgctcgcta tagcggttcc ccattgaaat
    43321 tctctttatc tgagatcaac caaaaaaaag gcgtatggat cgttgacctg acaacaggcc
    43381 tggagttgaa ttttagggag atcgaacccc tgcatgatat tgaagaaata acagcgagtt
    43441 tcaaagaatt gctggatcct gccttttatc agtccgtcaa tcgggagaat tacatctata
    43501 tctatctcac tgatagaacg atcatcccga atatgatgaa ccagcttcgg caaatatatc
    43561 ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag cgcaaacgaa
    43621 aagtactaga aatgaaagat cctcaaaaat tagcagaagc attttttagt gaaatgacgg
    43681 aagagcagct aactgcaaac caagaaaaat ggctcggaga aacgttgcaa gaaatcatgg
    43741 aaatggaatg atcaagaatg aaaccgaaaa aattacaact taaaaatttt ggtcctttta
    43801 tcaatgagac cgtagatttt tcacgtttga cagaagcacc gctttttttg atcagcggga
    43861 agacaggagc tggcaaaacg acgatttttg atgggatcac cttcgcactt tttggtgaaa
    43921 cttccggaag acttcgctct ggaaaagaga tgcgttcttt gtttgctaca ccagacgaag
    43981 aaacaagcgt gactttttct tttgaacatc agcagatgag ctatgaaatc gaacgaaaac
    44041 cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca gccaaagtaa
    44101 cgttgaccat ttttgatggg aaaggaaaag aaataaaaca aatcacaaaa cgtacagaag
    44161 cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa acagttttct caaatcgttt
    44221 tactacccca aggagaattc cgtaattttt tggtttcatc aagcagtgat aaagagactg
    44281 ttttacgtca tttattcggt actcaatttt tccaacagtt caatgagcga ttaaaagaaa
    44341 aagcaaaaca acagcaacaa aaattggatc atttagagca agagctgcat cttctgcaaa
    44401 aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg gtttgaagcc gttttagccc
    44461 aatggacaaa ccatcaggca atactcgaaa aacaaatcac ggaagaaaaa gaagctctaa
    44521 cggaacttca ggaaaaacaa aagcaactag aaaattccta ctatcgtttt gaaagcttga
    44581 aaaaaagtta tgaagaaaaa acgatattaa taaacaagca aaaggaatta gctgctcaaa
    44641 aagacgaaat agaggaaaag aaacgatgga ttgggtatta tgaatttacg caacgattag
    44701 tcgaaccaat ccagcgccta caagaaataa aagttgaaaa aatagaactg tcacaaaaaa
    44761 gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa ttatgaagag tggcaaaaaa
    44821 atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga agctgctcaa aaactgcaaa
    44881 acaaacagat gatggtgcca attgtcgaat ccttacgtag taaaaaacaa gacgcagaac
    44941 aattaacaat ttcattggat aaagaagaat tgaatgccga gaagctgtat caaaatctcg
    45001 ctatctatca ggaaagaact cgaattcttc aagaaaattt actggatcag gaaaaactgc
    45061 aagaagatcg tcttgctttt ggacagttaa aatatctaga tgaaaagcag caagaaatag
    45121 aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat aagagaagaa ctcaaaaaag
    45181 caactgaaga aataacaaaa aatagtcagc tattgaaaga acagacagaa caagcagaac
    45241 agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct tcagcttttt ttgcgtccag
    45301 gggagccatg tcttgtttgt ggatcaacgg aacaccccat acaacaatta aaacacgaac
    45361 agccaaataa ggaagatatc ttaaaagtag aacaacaact ggcagaagct gagtctgctg
    45421 tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca tgaaaataat cttcaacaac
    45481 aattatcaga tttagaacaa aaaatcatgg caacgaatat acaattggct gaacaagaag
    45541 aacacttgca acaggctttg aaaaaggcat ttcctgagat aacagaacaa aaaacaaaaa
    45601 caactcttgt agtaatcgaa caatctttag atagacgaaa aagcaagatg gccaatgcaa
    45661 gaatagagct ggaagaaata agacaacaag cagaaaagct cgaacaacaa ttagctgtct
    45721 acaaacagca gcagacagaa aaaaataatc agctccagca actgcaagga gaaatcaaaa
    45781 gtttagaaca acaattgcaa gatacggata gtaactgttt agaagaggag atacgacgac
    45841 ttgaaaaaca gttagaagaa gactcgaatc aactagctgt tgagcagaaa gcaggagtgc
    45901 aattgttgca agatcttcat acgcttcgga cacagcttga tcttcaacat gaacaaatag
    45961 aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat agctaaaaag ttagaaagcc
    46021 agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc acaacagcaa gcacaatata
    46081 aagaaaacca acgtacagtt acttcttatc atgaagagca attagtgaca gctgaccggc
    46141 tgcgtcaatt agaaaatatc cagctaactg agacattccc agatacagaa gagatcagcc
    46201 aacagttaga agaaataaga cgtatgatcg aaataaagca agaaaagata tacggtttac
    46261 aagaacaaaa aattagtaat caaaaaatct acgatgaatg tctttcgatt tatcacacaa
    46321 gccagacgaa attagatgag cttacgcaat tacagcagct ttcgcaaaca ttcaacggag
    46381 aaaatcctaa gaagactagt ttggaacggt atgtgcttca ggtttatctt caagaagtgt
    46441 tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa tcgttatcaa tttgagctag
    46501 cggataccat tggcagttat cgagggaaaa ccggattaga aatcaatatc tatgatgatc
    46561 aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg tgagagtttt attgcagcac
    46621 tttctttagc attagctctt gccgatgtga tccagacaca agctggtggt gtagcaatcg
    46681 atgcattgtt tattgatgaa ggatttgggt cattagatga agaagctttg gaaatggcaa
    46741 tggaagcttt agaaacgatc gaaagtgaag gaagaatgat cggcatcatt agtcatgtaa
    46801 gagaattgaa agaacgagta ttacaacaaa taagaattga tacagaaggt agcggacaaa
    46861 gcaaaatacg ctatcaactt ggataaaaag ggagattggt aactatgaaa tggagtattc
    46921 cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga aggccgagtg ttatcggttg
    46981 tccctgacca agaagaaaac gtgtggcatg cagaagtctt gggcagtgaa ttgtatatgg
    47041 ttgatttgga tggtacagca aaagagatag attactgcca atgtccctat tgggaggaac
    47101 ataaatattg taagcataca gttgctgtgg aactttattt acggtcgaaa aatctatctc
    47161 gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa agcaacaagt gagggagaaa
    47221 tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg gtgcaacctt
    47281 tgattattga ataccaagta gaaacgattg agactaatca gtatcaccca gagctttcta
    47341 ttttggcact ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca tatatcgtaa
    47401 aaaacatcta tgatttttta caagcttaca cagaaaaaga aacttacact gttaataaac
    47461 agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa atactcacgc
    47521 atcttgcagg aagtgctcag acgcatcaat tattaggtac gaacggctta caagtcaaag
    47581 gaaaactaga taaaagatat ctgcttctcc cagtagaaca agcccgcttc ctgttagagc
    47641 agatgaatca gactacacgt ttgcatctgc agataaatga acaaaacgat caaggaatac
    47701 aattttccga tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga gaagattatt
    47761 tgctgcaagc attgaatgat tttgattttt tctttatgca ttatcagtgg ggagttatcc
    47821 aagggacatt ttattctctg acgaaatatc aacaaacggt ttatcaaact tggaaacaat
    47881 tgattcgccg attggagaaa ccagaagtag tgttcaagaa aaaagaatta ccgtctttgt
    47941 ttaaagagat tatccctctg ctgtcagaga tcggcgacgt agctattgcc cctgcagtag
    48001 aagaggaagt agcagatatc cccgtcgaat ttattttctt tttccgaaaa gcaaaaggaa
    48061 aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat ctattcgact gacccgaagc
    48121 atgaggtctc gacagaacag gcttttcagg tgttacgtga cacagtagaa gagaaacgag
    48181 tacttgatct attcagaatg tatcgctacc agaaaaatga aactggttat gaacgtccat
    48241 tgccaagcgg ggaagaacta tatgcctttt tccgcacaga actatctgtc ttccgtcagt
    48301 ttggtgaagt cagacttgga agaaaattgc gagagttgta tttagatgcg cataaacatc
    48361 aaccgaaaat cgaagtaacc gagaccggtt catggctcga tattcgattc gatgtgacag
    48421 ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat gatgcttttt
    48481 atacattaga aaatggtcag gttctttctt ttgattcaga agaatttcaa caaacaagcc
    48541 aagtgttgca acaattacgg gaatcgatac gtacagaaga aggaacgatc catgtaccta
    48601 aaaatcaagg attgatcatt caaaatcaat tggaaaaaag caatgctact tttagtgaat
    48661 cgttccaaac gatggtacaa gatctcattc atccagaaag ataccaggca cagcttccca
    48721 aaggattgaa tgcaacgatg agagattatc aaaagcaagg ttttcgatgg ttgaagatgt
    48781 taggtcatta tcagtttggc ggtattttag ccgatgaaat gggacttggg aaaacactac
    48841 agacaattgc ctttttactt tcggaaaaag aagaacggaa aagcttctct gctttgatcg
    48901 tagcgcctgc gagcttaatt tacaactggc aggcagaggt gagaaaattc gccccttctt
    48961 taagtatcca agtaatcaat gggaataaaa aagaacgtga agaattactg gctaaagaca
    49021 cagatatccg agtgacttct tatgccagct taagacagga tctagctaat tatcagtcgc
    49081 aaaaaattga ttatttgatt cttgatgagg cacaaatggt caaaaatagc agtacgaaaa
    49141 ctgcacaagc attgagagaa ttagctgtac ctcagcggtt tgcactgagt gggacaccaa
    49201 tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac gattatgcca ggatttttcc
    49261 cgactaaaac aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg attcgaccgt
    49321 ttattttacg gcgagataaa caaactgtgt tgaaagactt gccggaaaaa acagagatga
    49381 atctatattc tgcacttaca gaagaacaaa aaactgttta tttagcttac ttgcgtcaaa
    49441 tgcgtgaaga aatcacatca atggattctg aagcattcaa gaaaaaccgg atcgggatac
    49501 ttgctggtct gacaagactg cgacaaattt gctgcgatcc tcgtctgttt attgaagatt
    49561 accaaggcgg ttccggcaag ctcgaacaag taaaagatct actggtagct gccaaagaga
    49621 acaaacgaag agttttgctg ttttctcaat ttacagggat gctgactatc ttgcaagaag
    49681 agttagccga gctagggatc tcgacatttt atttaagagg cagtacgaaa ccacaagatc
    49741 ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg atttccttaa
    49801 aggctggggg aactggattg aatctcacgg gagcagatac ggtcatcttg tatgaccttt
    49861 ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc acatcggatc ggacaaaaaa
    49921 atgtggtaga agtgtggcgt atgatctctg aaggaacgat tgaggaacgc atggattcac
    49981 tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa gaacagttga
    50041 cgaaactgac tgaagaagac atccgcatga ttttaagtgt aggagaaatc gaggagtaag
    50101 agactcgaaa aagcgttcag taatgggtaa gaaagccttt ctaaagaagc gattgtttat
    50161 ttgatttaaa aaatagcaag gaggctggga ttccatttta tcccagcttc cttgctatca
    50221 tttttttatt tctattttta aaggttatac aaagattgtc ttcattgtct ctaacagctg
    50281 atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt tcaattttgc gttctcgatg
    50341 gttgaaccaa agcgcttgcc agttagcact ttttgcacct agaacatcat tgtcgtaatt
    50401 atcgccaacg tataaggtct cttctggaag catatgaaat tctttctcag ctagctgaaa
    50461 aatctctttt tctggttttt ggaaacctgt tgcttgagaa atgagcatat tgcgggaagg
    50521 aatccaattg ttcaattgaa gttgcttcag tttttttgtc tgatggtcag tcggcccatt
    50581 tgtgataatt cctaatggga cattcttttc cttcaagaaa tcgagggttt ttttgacctc
    50641 ctcatgcaag cagatattat caagttcttc ctcgtaaatt ttttgaaata agaggccatc
    50701 ttcttccgta atatgagggt agtctaaatc ttttagtgat tggctgatcc gatgtgctcg
    50761 catatattct agtgtccaat ctcctgccat tacttttggg aagttttcat cactatgatg
    50821 gcggaaccgg atatatagtg gatgcatatc tgcatctgaa acaagcggaa cgactctttt
    50881 gaccgcgttt cgaaatggct gctgctgatc atacatcgta tcatcaacat caaacacaac
    50941 tgatttaatc aaaattttac acgcctttcg gttttcattg attttttcac aaatccttga
    51001 ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat aaaggttttt cttactatat
    51061 ttctatttcc ttgacagaat aaggttttta tatttcagtt tttttcacta aaaatcgtga
    51121 aacacgtcaa taagtttgat gaacaaatta atcattatgg tagaagtcga ttgatttatt
    51181 ttaatgcaat ctctgtggaa ttttcgaaag atttattttt
//
LOCUS       DAMYXM010000015        46081 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.015, whole genome shotgun sequence.
ACCESSION   DAMYXM010000015 DAMYXM010000000
VERSION     DAMYXM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.015"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            512..1069
                     /locus_tag="PNS21_001000"
                     /pseudo
     CDS             512..1069
                     /locus_tag="PNS21_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1127..3166
                     /locus_tag="PNS21_001001"
     CDS             1127..3166
                     /locus_tag="PNS21_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HDI5158724.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3275..4426)
                     /locus_tag="PNS21_001002"
     CDS             complement(3275..4426)
                     /locus_tag="PNS21_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDI5158725.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4580..5110)
                     /locus_tag="PNS21_001003"
     CDS             complement(4580..5110)
                     /locus_tag="PNS21_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDI5158726.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5209..5559)
                     /locus_tag="PNS21_001004"
     CDS             complement(5209..5559)
                     /locus_tag="PNS21_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HDI5158727.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5716..7245)
                     /gene="ahpF"
                     /locus_tag="PNS21_001005"
     CDS             complement(5716..7245)
                     /gene="ahpF"
                     /locus_tag="PNS21_001005"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDI5158728.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7255..7818)
                     /gene="ahpC"
                     /locus_tag="PNS21_001006"
     CDS             complement(7255..7818)
                     /gene="ahpC"
                     /locus_tag="PNS21_001006"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDI5158729.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7962..8624)
                     /locus_tag="PNS21_001007"
     CDS             complement(7962..8624)
                     /locus_tag="PNS21_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI5158730.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8808..10454
                     /locus_tag="PNS21_001008"
     CDS             8808..10454
                     /locus_tag="PNS21_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDI5158731.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10445..11527
                     /gene="uxuA"
                     /locus_tag="PNS21_001009"
     CDS             10445..11527
                     /gene="uxuA"
                     /locus_tag="PNS21_001009"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDI5158732.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11635..13188)
                     /locus_tag="PNS21_001010"
                     /pseudo
     CDS             complement(11635..13188)
                     /locus_tag="PNS21_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            13336..14477
                     /locus_tag="PNS21_001011"
                     /pseudo
     CDS             13336..14477
                     /locus_tag="PNS21_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(14603..15682)
                     /locus_tag="PNS21_001012"
     CDS             complement(14603..15682)
                     /locus_tag="PNS21_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDI5158733.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15791..16231)
                     /locus_tag="PNS21_001013"
     CDS             complement(15791..16231)
                     /locus_tag="PNS21_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDI5158734.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16288..16698)
                     /locus_tag="PNS21_001014"
     CDS             complement(16288..16698)
                     /locus_tag="PNS21_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDI5158735.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16879..17313)
                     /locus_tag="PNS21_001015"
     CDS             complement(16879..17313)
                     /locus_tag="PNS21_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI5158736.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(17413..18261)
                     /locus_tag="PNS21_001016"
     CDS             complement(17413..18261)
                     /locus_tag="PNS21_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDI5158737.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            18650..20416
                     /locus_tag="PNS21_001017"
     CDS             18650..20416
                     /locus_tag="PNS21_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDI5158738.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            20518..21777
                     /locus_tag="PNS21_001018"
     CDS             20518..21777
                     /locus_tag="PNS21_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDI5158739.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            21924..23225
                     /locus_tag="PNS21_001019"
     CDS             21924..23225
                     /locus_tag="PNS21_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDI5158740.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            23228..24079
                     /locus_tag="PNS21_001020"
     CDS             23228..24079
                     /locus_tag="PNS21_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDI5158741.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            24092..24907
                     /locus_tag="PNS21_001021"
     CDS             24092..24907
                     /locus_tag="PNS21_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HDI5158742.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            25058..26224
                     /locus_tag="PNS21_001022"
     CDS             25058..26224
                     /locus_tag="PNS21_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI5158743.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            26454..27101
                     /locus_tag="PNS21_001023"
     CDS             26454..27101
                     /locus_tag="PNS21_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDI5158744.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            27185..28129
                     /locus_tag="PNS21_001024"
     CDS             27185..28129
                     /locus_tag="PNS21_001024"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HDI5158745.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            28264..29715
                     /locus_tag="PNS21_001025"
     CDS             28264..29715
                     /locus_tag="PNS21_001025"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HDI5158746.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            29736..30572
                     /locus_tag="PNS21_001026"
     CDS             29736..30572
                     /locus_tag="PNS21_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDI5158747.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            30572..31231
                     /locus_tag="PNS21_001027"
     CDS             30572..31231
                     /locus_tag="PNS21_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDI5158748.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            31357..32046
                     /locus_tag="PNS21_001028"
     CDS             31357..32046
                     /locus_tag="PNS21_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158749.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            32052..33008
                     /locus_tag="PNS21_001029"
     CDS             32052..33008
                     /locus_tag="PNS21_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDI5158750.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            33052..33564
                     /locus_tag="PNS21_001030"
     CDS             33052..33564
                     /locus_tag="PNS21_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5158751.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(33820..34368)
                     /locus_tag="PNS21_001031"
     CDS             complement(33820..34368)
                     /locus_tag="PNS21_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI5158752.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(34457..35638)
                     /locus_tag="PNS21_001032"
     CDS             complement(34457..35638)
                     /locus_tag="PNS21_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDI5158753.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(35874..37382)
                     /locus_tag="PNS21_001033"
     CDS             complement(35874..37382)
                     /locus_tag="PNS21_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDI5158754.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            37541..37732
                     /locus_tag="PNS21_001034"
     CDS             37541..37732
                     /locus_tag="PNS21_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158755.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            37935..38852
                     /locus_tag="PNS21_001035"
     CDS             37935..38852
                     /locus_tag="PNS21_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HDI5158756.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            38845..39918
                     /locus_tag="PNS21_001036"
     CDS             38845..39918
                     /locus_tag="PNS21_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158757.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            40066..40584
                     /locus_tag="PNS21_001037"
     CDS             40066..40584
                     /locus_tag="PNS21_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158758.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            40565..40957
                     /locus_tag="PNS21_001038"
     CDS             40565..40957
                     /locus_tag="PNS21_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HDI5158759.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            41033..41968
                     /locus_tag="PNS21_001039"
     CDS             41033..41968
                     /locus_tag="PNS21_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDI5158760.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            42101..42880
                     /locus_tag="PNS21_001040"
     CDS             42101..42880
                     /locus_tag="PNS21_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDI5158761.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            42873..43190
                     /locus_tag="PNS21_001041"
     CDS             42873..43190
                     /locus_tag="PNS21_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDI5158762.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            43434..45044
                     /locus_tag="PNS21_001042"
     CDS             43434..45044
                     /locus_tag="PNS21_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDI5158763.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            45201..46049
                     /locus_tag="PNS21_001043"
     CDS             45201..46049
                     /locus_tag="PNS21_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDI5158764.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        15290 a         8292 c         8856 g        13643 t
ORIGIN      
        1 tataaaaaaa cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt
       61 tgcaacggga ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta
      121 aatttcacta tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac
      181 tcgtaaatag aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt
      241 tgcccagaaa cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt
      301 tccaatagct aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg
      361 atgctaggtc agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac
      421 tgacttattt gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag
      481 gagatgcggt gaaaaaagat cgattggtgg aggtcgctcg gattcacttc tggttgcaca
      541 atacaaccag aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga
      601 tattccaggt tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt
      661 attaaatcaa acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac
      721 atttcaagat tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc
      781 agaaaaatct ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg
      841 taatacttta ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat
      901 cagaaggcag cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat
      961 attcaagcta cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga
     1021 aagtgtgctt ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt
     1081 ataaatagtg aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga
     1141 caagcacggc aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc
     1201 tgcgggtggc ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg
     1261 agaaaagaca gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc
     1321 agaaatgaca tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga
     1381 taaataccag aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa
     1441 agtagataaa aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc
     1501 attaggtgaa ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac
     1561 cacgatcaac tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag
     1621 cctgaccaca caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc
     1681 aacaacaggc aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga
     1741 aaaaacagcc aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa
     1801 tcaggcgatt tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga
     1861 tggagcaacg ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta
     1921 tcctttgggt gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga
     1981 tattgataag aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat
     2041 ggcttttgat aaggatcttc gtgggacaac aggtgggaaa ttaagcatta cagatgcaga
     2101 cggtgtagag aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt
     2161 gacaatcgat gcgaaggcac aaaaaatagc ttttgacagt ctaggaggaa aagctgcatc
     2221 aactgttgcg acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta
     2281 tgatccaaac aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa
     2341 tcctgaacat ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa
     2401 aatgattaca gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac
     2461 gatcaacggg cttaaatggc aaaaagacag ttcttgggga tcttttaaaa caacgcgtgt
     2521 cagtagtgat gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata
     2581 tgcggcacaa gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt
     2641 catttttggt gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga
     2701 agatagcttt aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct
     2761 aattactcct atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt
     2821 ctatcctaaa ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac
     2881 agcagtacaa acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc
     2941 acattctctt tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa
     3001 agaaaaacag gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa
     3061 ccaaggttat atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa
     3121 acgagcatcg gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta
     3181 agtacagtaa aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc
     3241 acgatgtctc tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa
     3301 aatatttgag aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg
     3361 aatagtactg tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca
     3421 tttctccacg cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg
     3481 caatctgagc accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata
     3541 tagaaataaa gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga
     3601 caaaaaatac tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt
     3661 ctttaacctt tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa
     3721 gcccaacaag atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa
     3781 tagaagaatt tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga
     3841 ctaataagaa aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag
     3901 cattgctaga agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta
     3961 aaatgtcatc taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt
     4021 ttagttcttt taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa
     4081 agaaaatcgc ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa
     4141 ccgggaagat gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa
     4201 gagataaatc actttccaga ccagctaaaa acattaataa gatcacacca atctccgaaa
     4261 agtcatggac tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac
     4321 caacaagtaa ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa
     4381 tcgttgttga aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt
     4441 tgtataaaat atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata
     4501 ctcacaaaaa aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta
     4561 ttttaataac taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa
     4621 tctcctggat gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac
     4681 ttttgaatgg attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa
     4741 cactccaact tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga
     4801 taacagctaa tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga
     4861 cgatcagagc taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg
     4921 ttttcctttt aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg
     4981 cggtgatcag ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc
     5041 cagaaataat ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg
     5101 tctcaaacat ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca
     5161 aattaaatgt tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag
     5221 gatcttttcc atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat
     5281 ttctttcatc aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat
     5341 caaggaacgt gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg
     5401 tttttgtagt tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc
     5461 atctacttcc tcttttatac gatcttttct ctccgctttt gctacataca taggataaac
     5521 gcttgcaaaa cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt
     5581 tcaaattatt tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa
     5641 aaacaaaaag gattgtgaca aaattcttgt caccatcctt ttttccgctc tctgactttt
     5701 tacaatcttc atagctcaag tgcgcatcag ataatcgaat gctcctaaag ctgcagtagc
     5761 tcctgaaccc attgaaacaa tgatttgttt gtatggacta tctgtacaat caccagctgc
     5821 gaaaacgccg gggatattcg ttgcgccatt tttctcggtc actatttcat ttcttggatt
     5881 catttcgatc gttctatcta gccaatccgt attagggagt aagccgacta gaatgaatac
     5941 tcctgaaaca gctagtgtat gcatctccat cgtacggcga tcgatatagg agagagattc
     6001 tacctgattc gtaccgctga tttctttcgt cgctgcatgt ttgataacag tgacattcgc
     6061 aagagagact agtttatcct gtaagatttg atctgctttt aattctggta agaattctag
     6121 aacatataca tgttttgcta acccagccaa atcgattgct gcttcaacac cagaattacc
     6181 accaccgata acagcaatct cttttccttt aaataaaggg ccgtcacagt gtgggcaata
     6241 agcaattcct ttatttttaa actctttttc gcctggtaca ttgatagaac gccagtgcgc
     6301 acctgtcgcg atgatggcgg tttttgcttt aagtgatata ccgttagcta agccaatatt
     6361 aataaaatgt tgctctttat ttaaggaaac ggcctgttga ttcatcataa tgtcgatagg
     6421 gtaactacgc acatgacttt ctacttgctg catcaatttt ggaccttctg tgtaagaggt
     6481 accaatcata ttttcaatcc ctagtgtttc aacgacttgt ccgccgaaag aatcagcgac
     6541 aagtcctgta cggattcctt tacgtgctgc atagatagct gcactactag ctgctggtcc
     6601 accgccaatc actaatacat cataaggttc tttttcaaac aactcttctt caatcaaagg
     6661 gccagtgatt ttttcaacaa tttgttcgat cgtcatgcgt ccgctactaa attcttctcc
     6721 atttaaaaac acagttggca ctgccatgat ttttttatca tcgatttctt tttggaacat
     6781 cccaccttca atcatcgtat gagtaatatt tggatttaat acagctaata tattcaatgc
     6841 ctgtacaacg tcggggcaat tatgacaagt caagctaaca taactgtcaa aatgcaatgt
     6901 ttgtgttatt tttttgattt taagactcaa agattcactg attttcggtg cccgtccact
     6961 aatttgtaac atagccagta aaaaagagga aaattcatga ccgaggggga taccagcaaa
     7021 aacgattcca ctttctttgc caactggatt gatagaaaaa ctaggtgtgc gcatcgaatc
     7081 tcttttttcc caagagattt taggagacat ttctgctatt tccttcgtaa attcagctag
     7141 tttttgacta ttttcactat tatccaaact aacagaaaaa acaataggag attcaatcag
     7201 tgctaaatat tgttcgagat tttgtttagt ttctttgtct aacatatcca ccacctaaat
     7261 ctttccaaca agatcaaaac ttggtttcaa tgtttcgcca ctttctttcc attttgccgg
     7321 acaaacttca cctgggtggg tacgtacata ttgagcagcg cgaatcttat caatcagtgc
     7381 actagcgtca cgaccgattc catctgcatt gatttccata gcttgaacga ttccgtctgg
     7441 atcaataata aatgttcctc gctgagctaa tccttgctct tcatccaaaa catcaaaaat
     7501 acgagaaatc tgatgagacg gatcaccgat catcacgtat tcgattttac cgatagcatc
     7561 tgatgtatca tgccatgctt tgtgagtaaa gtgggtatcg gtagagcaag aaaagacttc
     7621 aacacctaaa gatttcaatg tactgtattg ttcttgtaag tcttctaatt cagtaggaca
     7681 aacaaaagta aaatcagctg gatagaagca aagaatactc cagttcccca taagatcttt
     7741 ttcactgact tctaaaaatt ctccttgatg ataagcatgt gcattaaatt cttcgatttt
     7801 tttcccaatc aatgacatag taaaacctcc aataataatt atttatttat aattattgta
     7861 atttagttgg agttgcaaag tcaatggata ttttgttatt taataaagat tactcaaaaa
     7921 cgaaggttaa ttataaaaat agaagaggtc gcaagaaaat gctactgctg gaaatactcg
     7981 ggataacgtt gagtaacagt ttcctgttct ttcagcatca tgtgtaagtg caatcccgtt
     8041 tgaaatcgag cttcttctgg ttttttatca actaaagcct tgagtaaatc ataatgttgt
     8101 tccatgatcg tttcccatgg aagcccctga atggtcactc gtaaccagcg gaaacgttct
     8161 aaatgggtat tatggtcatc taaccagttc caaacttctt ttcttcctgc aatttcgaaa
     8221 caagcttcat gaaataaatt atctgcacgg aaaaaagcgc gttcgtcatt tattttgact
     8281 gctttttctt gttcttctaa aatcgtttct agcactattc gtgtttgtgt gtccaatttg
     8341 gaacaacatt caatcatgat ttgctcttcc aactgttctc ggacaaagcg agcattttct
     8401 gcagaaacaa gattaatttt ggaaacatac gtaccacttt gaggtatcgt atacacgagt
     8461 tcttgctgac gtagttgaat caatgcttcg cgaatgggag tccggccgat agcaagatgt
     8521 tcctccagtg ccttttcaga tattttcctt ccaggttcta agtcagcata gataatttgt
     8581 ttacgaatcg cttgataagc ttgtgattgc aagctgcttt tcatctttat tcctccaaaa
     8641 tcatttctat gaacgtagta taacagaaga ggtctgaaaa acaatctggt atgtcacatt
     8701 aactagtaaa tttgtataaa tagtaaaatt ttggttgaca tagcgatttt tattgcataa
     8761 aattcacttg aatacaaaaa ctaatatgtt agtttaggag gggaaaaatg attgatttaa
     8821 acaactggag gaactttatc cctgaattat ctcaaaaaga agtagaaata cctcattttg
     8881 accgtgcatc attaagagaa aatggacaaa aaaatcctgt ctggatccat ttcggtggcg
     8941 gaaatcttta tcgaggattt catgcagaaa tcgctcaacg tttagcggat ctaggcgaat
     9001 tgtccactgg tgtcattgtt tgtgaaacat tcgatgaaga aataattgaa agggcttacc
     9061 aagattttga tgatttgatt cttgaagtgg tcatgccaag cagtggagag ttcgtcaaac
     9121 gtatattagc tgcgacagct gacagtctgt attgtcatcc agattgttct gaacactttg
     9181 ataagatgat tcgacacttt gaaaatccta gtttacaatt agttactttt gctattactg
     9241 aaaaaggtta tcgattgaaa gatacaaata ataaatatat aggaccgatt gcagtcgata
     9301 taaaaaatgg tccaaagaag ccatcacata caatgagtat tcttgctgtg ctactttata
     9361 aacgtttttt atctggagct tggccgattg cacttgtctc tacagataat ttttcacaaa
     9421 acggtagtca attacgtact tctttgctaa cgatcgcaaa agaatggcaa aaggaaggtt
     9481 atgtacaaaa tacatttatc caatacttat cgaatgaaga aattgttgcg tttccatgga
     9541 cgatgattga tcgtatcacc ccgaatcctt ccttgtcagt tagcaaaatg ttggcaaaag
     9601 agggcttttc aaatatgtcg attttccaaa ccgcaaaagg aaataatatt gctccatttg
     9661 tcaatacaga ggacatccat tatttaatca tggaagatca ttttcccaat ggacgtccga
     9721 aattcgaaaa agctggggta ttcttgactg atcgtgcaac ggtagataaa gcagatacga
     9781 tgaaggtgac gacttgcctt aatcctttgc atacgtcttt agctattatg ggttgtttac
     9841 tggggtatag gagcatagct gaggagatga aagatccaga tttatcgctg ctagtttctg
     9901 aaatcgggta caatgaaggc ctcccagttg tggagtctcc aggtatcatt gatccaaaga
     9961 tctttctgga agaagtaatt actgtgcgct tttctaatcc aaatattcct aatatgcctc
    10021 agcgaatcgc cacggatacc tctcaaaagg tggcgattcg ttatggtgag acgattaaaa
    10081 aatatatgga aatagaagtt aaagacccgc agacacttgt attcatacca ttgactctcg
    10141 ctgcttggct tcgatacctt attggcattg atgatcaagg aaaaccattc atcccgagtc
    10201 cagacccact attgacagac cttcagcata agctttccga tattcaactg ggtacgcaag
    10261 atacatcaca tattcatgag aaaatggctc ctattttgtc aaataaagaa atttttggtg
    10321 tagatcttta ccaagcagag cttggcagaa aaatagaaga aatgttcatc aagatggtag
    10381 caggaccaaa tgccgtacga aaaacattac aggattactt acaagaacat ggaggaaatt
    10441 tatcatggaa atgactttta gatggtacgg atcacaggaa gaaaagatta ccttagatga
    10501 aatcagacag attcctgcgg tcaaagggat tgtaggcaca ttgatggata taccagtagg
    10561 agaggtatgg cctagggaga ggatcaaaga actaaaagaa gaaattgagt ctcgtgggtt
    10621 aacactaaaa gtaattgaaa gcgtcaatgt gcatgatgcc attaaaattg gattaccttc
    10681 ccgtgagcag tatatcgaaa actataagca gacaatccga aacttggcag aatatggaat
    10741 agaagtcatc tgctataatt ttatgcctgt atttgactgg gtgaaatctg accttgacta
    10801 ccgcttagaa gacggctcat ccacgttagc ttttatatct gatgatattc cagatgatcc
    10861 aagagaaatc gtcgcgcgca tcgaacattc cagcaatgaa tttggattgc cgggatggga
    10921 acctgagcgc ttagctccta tcaaatcctt gcttgaagcc tatgcatcag tagacgaaga
    10981 aaaacttcga gaaaattttg cctattttct ccaatcaatc atcccaacat gtgaagaggt
    11041 aggggtcaaa atggctgtac atccagatga tccgccatac tcgatttttg gattagctcg
    11101 aatcgtaaag aatcgagaag atttagattg gttgtgtcgc atagtagaca gcccttataa
    11161 cggaatcact ctttgtacag ggagtattgc agaagatcca gaaaatgatg tatatgggat
    11221 tttagcagaa ttttgccaaa gagatcgaat cccatttgca catgttcgaa atattaaatt
    11281 tttagctaat cgagatttct atgaggcacc acataaatcc gaatacggtt cattagacat
    11341 gtaccgcata ctaaaaacaa tgtatgacaa cggatttaac ggatacatcc gtcctgatca
    11401 tggaagaatg atctggaatg agacaggaag accaggatac ggcttatatg atcgagcatt
    11461 aggtgctgtt tatctaaatg gattatggga agcaatcgaa aaaggaaaca aaagtaaaga
    11521 taattagtga aaaaaactgt aattttatta tgtaaataag aaggatgttc aaattcttga
    11581 tacataaatc cggaggttgt taataagcat tcggatttat gtatcttatt tggtctagga
    11641 agtaacttga ttttcttttt tcgattgatc catgagtgct tcttctttta attcaaagat
    11701 atattctgag atcctcataa tcactttttt ccagtcttct ggtgtgatga ttccttttaa
    11761 aatatagatt tttttgatat gagaaggcaa ttgtttcaaa gggcagtcgc tgataacgat
    11821 gttgcagttc tccataaaat cattttgtat tttgatcgat tcatttgaaa aaatttgttc
    11881 tatcttaacg atcagatctt tactgatata aaaatcacgc gtatagttca caccaatcac
    11941 aattcgcggc tgttttttgt agcgatcata caaatgtact aggtcttcaa ccatccattg
    12001 cacattttcc ttttctaaaa taactggtaa caagtgttga ttttgttctc tgaaatccat
    12061 ataaaattgt tcaatggcgg tttggacttc tttcaatacg ctatgttctt gataaagcat
    12121 gaaagtagga aggggcgtat ttggttgatt gaagttaaga aatccattat aaagtttatt
    12181 tcgtgcgaat gccaatgagt aaagaatctt ttctttttca ggtataaaca aatcaaatac
    12241 agtacaaagg gactccacca tttctcttga ataaagaaca tgaggaacgt ttgctttttt
    12301 taaaagtttg tattgctcca agcttgtttc ttccgattcg attttcgtga tagaaagtcg
    12361 agctaaaata ttcactaaga tgcgttgttc gcgacaaatt ggcagattag gtcgcaaaat
    12421 atctacagag agatcaataa aaaagtcaat atattcatac tctgtgaatt ctttttgtac
    12481 gtctttcaca gaagttgcag gaaaattctc tttacatatt tttaatagaa aatataattt
    12541 atctatcata ccgctattta gtcggtcaag tacttctttt ttaaaaaaag tattcatcat
    12601 aaaagccact acttgcggat cttcatcata aaattctgta gcagtacttg aaaagatgtt
    12661 ccaataaata tctaatgctg catattgaat ttgtacctct tttccagaaa aagactttct
    12721 gccattatta gaaaaggaga tagacaaacc gtactatgct aaaaatgcat taatcttttt
    12781 cattttcgta tacaaataag aggcactata gtttgtttgc tgactgaaac tgataatcga
    12841 atttgtctga cagctgaaca aagaagtcaa gatctgataa ctgactgatt cgttgcagta
    12901 tcttccaaat agacgataat agcagtaagc cggatcaatt ccttcaaaat tcaactttaa
    12961 aaaaggtttt tgcttatcaa tatgcagttc tgttagattg agacgctcaa agtcttgttc
    13021 taattctttg atgtatagta aaaaatttga ttcagacata tggaaatgat ctgctaaata
    13081 ttgtgtagta ataaagaaac gatgttcaga taggaaaaga aaaatatcta attttgtatt
    13141 gctttttttc ttcaagataa acgttttaat cgcttcttga gtttgcatga aagaggctcc
    13201 tttttactta aattgttaaa acttgaaaat attactgttt ttttcataaa tttagacaaa
    13261 aaaacagtaa acatataggt agaatctcta ctatcttatt tcttgcatac aaaaaaatca
    13321 aggagtgaga gagttatgag atattcaacc atcttaatat caacatttgc agccttaagc
    13381 ttatcgctta ctataaatca gacagtccac acagaggaga cagaaacagc caaatggaca
    13441 gaaaccaagt gggccaacgt caatcgaatt acctttcagg atgaattttc atccttcaat
    13501 tcaggagtcg caaaattaag aaaaggacaa acagcgacca tcaaagcaaa aattgactat
    13561 tcgggagact tagcacctat actacaatca gctgttacat ttgagaatgt tgatcccact
    13621 ttgtcgattg gctatgacga gaacactttg actatccaac acaagcaagc gatatttacc
    13681 ttcaccgtta cattaaatca ggacttgtta aaaccagcgc tttttactgt caaggtatca
    13741 gatggactac cgaacgatca tcatttaacg ccgtatagtc agcggcagac aattgagcaa
    13801 gattcagcca tcgactcagg aggagatctt gttgaagaac caacagataa accagaaaat
    13861 gaaaataagc cagaagtacc gcctacagaa aatccagacg gagaacagaa accggaaata
    13921 gaacctggag aagaacccga tacagaaact caaccagagc cagacaataa atcaaaacct
    13981 gaaataaccc ctggagaaaa accagatgta actccagaac cagatacgga ttctggaaac
    14041 caaacagtac cggaaacaaa tccagacaca gataatgaaa ctgaaaatcc agaaaaacca
    14101 gaggtagatc cagaagaaaa accagatgtg actccagaac cagatacgga cgcaagagac
    14161 caaggaatac cagaaaaaat aaacaaaaaa acaattcaag aggatggaaa aaaagagagt
    14221 aaaaaatcaa atctagcaat tctaaaaata aatgaagagc agctgaataa aaaatcacga
    14281 atatttgata gcgcccaatc agctgaaacc ttaaaaagta gcaaggatac aacctttgct
    14341 gtcctgaaac gaaaaataaa cagctaccaa aatccggaga gtcacaaaac aaagttatac
    14401 tgtggagcgg aatcattcta ttaagtatag caacaatgct ttctgcaaaa agatttaaac
    14461 aaaacagatc tctctaacat ttgaataatg tctgtgaacg catttttcaa ataaaaagaa
    14521 atatccagaa aatgacccca aaataccaga ttgaaatctg atatttgggg tcgttcatac
    14581 tttatctttc ttcatattta ttttaaggga tatctgtttc ttttggaaga tggttcggga
    14641 tctttttcac tttcaataca tctaaaaaga aggtaaagat cggaatgccg acaatcaagc
    14701 cccaaacgcc aaagaaccgt tcgctaacaa gtaagaccac aaatgtataa aaaatcggca
    14761 gttctgtttt gctggacatc aacttcggat tcagcacata agactcaatt agatggacaa
    14821 tcaaaatcgt aatcaggata taaacaacat ctcggatgcc tccttgggaa taagctatga
    14881 aagatagagg aatacaagaa atgataacac ctgctactgg gatcagactt aaaatgaaaa
    14941 tcatgatcgc taaactcagc agctgatgaa agccaaatac agccaaggcg attgtagtga
    15001 gcaacgtatt taaaagagcg acaacaaatt gagcttctaa taccaaacca aatgtattga
    15061 caaacttatc ggcaaaatag taaatatctt gaaagaacca agaaaattca ctttttaaaa
    15121 acaatcttga gaatacaatt gtttttttct tttcaatcat aaaaaagaaa cttaagatga
    15181 atgacatagc aaacgccact gccatttttc ctatatcctg taaatagcca agcgcaatcg
    15241 aagcgccatt ttgaagctgt tcaaaaaaat cgtttcgctc aatataagta tggatatatt
    15301 gtaaaacagg atctgcatct tttggctgat gctcgtaaaa atcaataacg gactggatca
    15361 tatcaaatgt ttgatgaata agcatcggga tatactttgt gaatgccaga tagataaggt
    15421 aaacgatcag tgtataaatc atgatagcta aaacacctgt agggattcgg acaaatcgct
    15481 gaatgatttg aacaagatga atcatcaaat aagtaaagat aaatgtgagc aaaatggttg
    15541 taatcatact tctaactaag aaaagaacaa aaacgacaaa taataaaaca gtgagacggc
    15601 gtattttttc attcgccaaa aaacgctgat aaacggtcaa atcgacaacc tccaaataac
    15661 aaaaaataaa cacgttattc attatatgat ggaatatgaa aataagaaag agaaagagca
    15721 aaaagaaaaa ccgctaaatg acgttatctt atgagttggt tttcctcata aaatcgttca
    15781 tctagcagtt tcagcccttt ttctggtctt gatgaaaatt atctagcatt tttcgggtca
    15841 gatgttgcag tgtatccaat tcttcctgtt caagtggaag ataagacttg atcttttgtg
    15901 gaataccttc tgcttgtttt tgcaacgcag caccttgagg agtcagcgta acatttacga
    15961 ttctttcatc acccgtttca cgctgacgat taatataatt tttttctgct aactttttta
    16021 gtaagggagt cagcgtacca gaatccagac acaaatcatt tcctagctct ttcactgaag
    16081 tctttttctt ttcccataaa actaacatca caagatactg ggtataggta agattcagcg
    16141 gcttgaggag ggaagtgtag tgtctaatga tttccttaga ggcggcatat aaaggaaaac
    16201 atacttgatt aactaaatat agatcttcca tttttccacc tccgctgttt tactatagtg
    16261 tatcatagcg gatttacaca aaaaagtcta ttcactaact gctttcagag taacttctat
    16321 atttccagct gtagcttttg aatacggaca cactttatgc gctgcctcta ataattcttg
    16381 cgtttcttcc agagaaagac cttcgataga tccttcaatc tctacaccta agacaaagct
    16441 cgtttcactt tctgaataaa gggaaacttg tgcactgaca gtagaagccc cagagatatt
    16501 tttttgctgt ttgatcaatt ctaatgcact gttaaaacat gcactgaaac cagctgcaaa
    16561 caattgttca gggttggttg cattttcgac gcgtttacca ggagctgcaa tattcaattc
    16621 gaatgtacga tcgggattat gaacttctcc agagcgtccg cctgtgttga tcattttcgt
    16681 agaatacatt tttttcataa aattaccacc tttaattata ttttatacaa ttgaatttta
    16741 cacaatataa ttgtggaaag caaagaaatg gattggcata agataaataa gagaaagcac
    16801 atgaaacaga cgcgctttct cgaatagcaa acatatacaa aaaaatatca gagacaaaaa
    16861 ctaatctcat cataaacatt acatatattt ctcaaaaagt tgttttcttt cttcaaaact
    16921 tacttcacga tggacctgat gaagcatcca taaataacca aaaggatctc tgaacattga
    16981 gttagtcaca ccaaagtcca tcatttctgt catttcttga atctctacac agccatttcg
    17041 taatgcttta ttatgtgtgt cctcgatatc agagacgatg atattgaacc agatcgtatt
    17101 tggatgttca gcagtaggag cagtcaaacc aagagacgtg ttttcatcca gcatatggaa
    17161 tctcgtgcca tacaaggtaa aaatgacttc attattgcct ctagggtaat ctgttgcctc
    17221 aatcagttgt atttcatcaa agattgtttg atacagttct agtgctttca aactatcatt
    17281 taatacaaaa tctatttccg ttcctttatg cataaagatc tcctctcctt ttcagtcgaa
    17341 cagttactag attcttttat tataccagtt aaagaaaacg cattcaaaaa aatagcggat
    17401 ttacttacca actcattcga ttggatgata agtaaatccg cgtcccatta cttctgctac
    17461 gctgctgaca atgacaaagg cacttggatc gatttcttct atcaatggtt tcattcggga
    17521 aaattcatga tcagtcacaa cgatcaataa aacctctttt gactttcgat tatacccgcc
    17581 ttttgcctct agcagggtaa ggccacgtcc aatctcagca cttaagcgaa ggcggatgtc
    17641 ttccaaataa tgttcactca tgaccataac agatttcttt cgattgatac ctgtttcgat
    17701 ataagtcatg acaattgaag tgattgcaat agataaaata gcataaagaa actcttctat
    17761 tccaaaaaca aacagattaa gagaaacaac gaatgcgtct gtaaaaaata agccaatcga
    17821 agtattcaag tggaaatatt ttttaaaaat gaggggaggg atcgtcgtac cgccgctaga
    17881 agagttgttt ttatataaaa tcgcaactcc taaagcaaaa atcgcacttc caaaaatgac
    17941 agacaacaac cgatctgaag taatcattgt ttctggaact attgcaatga ttaaaggaaa
    18001 aaacaagctt ccgaaaagta ctttaaaaaa gggtttttta cctaaaaata gaagagctag
    18061 taccaacata acgatattca acactaaaat aactatagct cggtcaaatc ctaaaacagc
    18121 ttccagtaat attcctagac cgctgacacc tccagcagca atatgatgag gccctaaaaa
    18181 catattgata gcgactccta atatgaagag ggcaaaaata atcttaatgt atgtccatat
    18241 aaattgtctc gtttctttca tttatccact tccttaattc attaataata ctcttatctt
    18301 attataactt acttttcacc ttaagttaac tttaattaat aaaaaaatta ggctagtcta
    18361 aattattatg tttcattctt ctagaatata cttctacgtt aaaaactgtt aaaaaacttt
    18421 tactttagaa aattggagaa attgatcgct taagataaat tcaggacact aaatagtaca
    18481 ggaagtagat taaaactttt aaaagtttta agtctatcta tttggcaagt ttaaaaaata
    18541 aaaattcggt atcaaaaaac tgttaccgat aacatttttg aaagcgttgt cttttgtttc
    18601 tctatcactt atagtgatga cgtcatatta aaacacaagg aggaaatcaa tggatacagc
    18661 tgcaatttat catcgaccgg aaagtgaata tgcatttcta tatacgaata caagatttcg
    18721 tattcggctt cgtactagga aaaatgatat caaaaaagta tatgtgcttt gtggagatcc
    18781 atataccata acaactgaaa aatggtatca aaaacaacga ccaatgaaaa aatgtctaag
    18841 taccaatgtt catgattatt gggaaattga agtgacgagc gaaacaaggc gcttacagta
    18901 tgcgttccat gttgttgggg aagataacac ggactgtttt tacggtgatc aaggaatttt
    18961 tccttatcga gaaaatgtga ttactgaacc aaatttttat tttcgaatac cttattttca
    19021 tcaaatcgat cgattcacta ttccagaatg ggtaaaagaa acagtatggt atcaaatttt
    19081 tcccgaaaga tttgcaaacg gggacaaagc aaatgacccc gaacacactt taccttgggg
    19141 aagtaaagat ccagaccgag aagacttctt cgggggagat ttgcaaggtg tgagggatca
    19201 tttggattac ttgacggacc ttggcgtcaa tggcatctat ttttgtccca tatttaaagc
    19261 cacatccaat cataaatacg atacgattga ttattatgag gtggatcccg cttttggcga
    19321 taaaaaactt ttaaaaaact tgattgatga agcacataaa agagggatac gtatcatgtt
    19381 cgatgctgta ttcaatcata tgggtgtcca ttcaccacag tggcaagacg ttctcaaaaa
    19441 cggagaaaaa tcgatctata aagactggtt ccacatccgt tttttcccag tagattctta
    19501 tcagatgact gacttaccag aaactgctga aaatatccca tatgatactt ttgcttttac
    19561 tccattcatg ccgaaactaa atacagcaaa tccagaagtg caaaaatatt tgctggatat
    19621 cgcaacttat tggataaaag aattcgatat tgatggatgg cgtcttgatg ttgctaatga
    19681 agtcgatcat catttttgga aaaaattcag agaagcagtt acagaaatca aaccagacat
    19741 ctatatcctt ggagagattt ggcattcctc tcaagcttgg ctgcaagggg atgaattcca
    19801 tgcagtgatg aattatgcat tcacagattc gattaaagat tattttgcaa aaaagaaaat
    19861 cacagcttca caaatggtca gtggaatgaa tcatcagcaa atgctttaca gagatcaggt
    19921 gaacgaagga acattcaact tattagattc tcatgatact gccaggatct tgaccctttg
    19981 ccaaggtaac aaagaattga tgaagtctgt gatggccttt atgtttcttc aaaaagggtc
    20041 tccttgtatc tattacggga cagaaatagg gatgactggt gaagatgacc cggattgccg
    20101 caaatgtatg atatgggaga aggaggaaca agatctagag ctattcggtt ttataaaaga
    20161 acttgtttct ctaaggaaac agttgtctaa gattatttct gaaggttcta ctcagtggct
    20221 aatagtatat gatcgtgaag ataagctcta tttcacacgg gaactagagg gacagatcat
    20281 ctatgtttac tttaatcagt caaaagaacc ttgggtagta gaacaggaaa atgaggtgat
    20341 tcttagtcaa aattgtcagt tgttagaaga cgggaaagca gagatacgac aatacggatt
    20401 tttgatcatg tgttgaaaac tgttaccggt aacaacaaga aagcgtttgc aaatgaagat
    20461 ttgtgcccat tataataagg gtgtaacagc acgttgaata cacaggagga aatgaagatg
    20521 aaaaaaagaa gtgtgaaaca gttgggttta gggttaatct ctttaggaat cttaggcgga
    20581 ttagcggctt gtggcaatgg taactcttca gcagaagggg aagccgatga aggaaaaacg
    20641 ttaacagttt ccgttgattc tggatataaa gattatataa acgaaataaa aaacactttc
    20701 gaaaaagaaa acgatgtgaa aatcaagcta gtagaaaagg acatgttcga tcagttggaa
    20761 tcattagcgt tagatgggcc tgctggaaag ggacctgacg ttatgatggc agcatatgac
    20821 cggatcggtg cgttaggaca gcaaggacat ctggcggaag taaaactggg aaatgaagca
    20881 gcttacgatg aaacagacaa agcccaagtg acttatgatg gaaaaatcta cggagaacct
    20941 gcagtaatcg agacccttgt tttgtactat aacaaagatt tagtcgatac agcaccatca
    21001 acgtttaaag atttggagaa tctatccaaa gacagtcgtt ttgcttttga gtcagaagca
    21061 ggaaaaaata caggattttt agctaaatgg actgactttt attattccta tggtttaatt
    21121 gcaggttacg gtggctatgt atttggtgat gatggcacaa atccttcaga tatcggtttg
    21181 aataatgcag gtgctgtaga aggaatctct tatgcaacag attggttcaa aaatgtttgg
    21241 ccaaagggaa tgcaagacat aaaaagtgca ggagacttcg ccagccagca attcatgtcc
    21301 aataaaactg cagcaatcat tgacggtcca tggcaagcgc aaacatacaa agaaaacaat
    21361 atcaattatg gtgtagccaa aattcctact ctaaataatg gacagcctta tcaaccgttt
    21421 ggcggaggaa aaggatgggt agtcagcaat tatgctaaaa acaaagacct ctctcaaaaa
    21481 tggcttgatt atgtaacgaa tcaggaaaat caagaaaaat tctttgaaat ggtaaatgaa
    21541 atccctgcaa accaacaagc tagggaaaca gcaaaggcta aaaacgatga attaacaaca
    21601 gctgtgattg aacaatatga aacagctcaa gcgatgccaa acatcccaga aatgggcgaa
    21661 gtttggacag gtgcagaaaa cttgatgttc gatgcagcat caggttcaaa aacaccaaaa
    21721 gaatccgcag atgaagcagt aaaaacaatc agtgaagcca tagaacaaaa atactgatta
    21781 aaatgataac cgtccatttt tataaaaaat ggaacaggtg cttcagataa aagtggcaag
    21841 agcgagatgg aaaattatct tgctcttgtt gccattatct taaaagcagt ttgcggttta
    21901 gcaagtttag agggggcatt cagatgcagc aagataaaac aagcaatatc aacgaaaaaa
    21961 aaatcacgct ttgttcactt attccaggga tggggcagtt tgtaaatggc caagctttta
    22021 aaggtatcgt ttttttcgcc atattcgtcc tattcattgg tcagatgctt ctgggcggat
    22081 ttcatgcatt ggtaggattg gttactttgg gaagtatacc aatggaagat cattcattat
    22141 ttattttgat tgaaggcact ttgcaattca tcattacaat cattttcttg attttttacg
    22201 gtttgaatct tttagacgct tatcgagtag cgaagatgcg tcaagagggc aaaaaaatca
    22261 atcggacgat gaaggaagtc atacataata taggtgatga aggttttcct tatctattga
    22321 cacttcccgc ttatcttctc atgattttta cgatcatttt cccagtccta gttacattgt
    22381 tcactgcatt tactaattat gacttcaatc atattccacc agccagttta attgattggg
    22441 taggggctaa aaactttttt agtatcttct ttttaagctc atatcgtaat actttttttg
    22501 cagtcttttc gtggaccgtg atttggactt tatgtgcaac aactttgcaa atcacccttg
    22561 gtattctcac agcggttgtt gctcaccaga aatttattaa attcaaacgt attttcggta
    22621 tcattttctt attaccatgg gcggtcccag cttttattac tatcatgagt ttttcgaata
    22681 tattcaacga tagcgtaggt gccatcaata ctcaagtgat tcctttcata aaccagcttc
    22741 cttttgtaga tatcagtgca atctcttgga aaacggaccc gaactggacg aaagtagcaa
    22801 ttatcttagt gcagggctgg ttaggatttc cctatatcta tgtgatggtt acaggaatct
    22861 tacaatcgat ttcagaagac ttatatgaag cagcaaaaat cgacggagca aatgcatggc
    22921 agcgcttttc aaacatcacg cttcctgctg tcttcctagt ggcagcgcca acatttatta
    22981 cacagtatac ggggaatttc aataatttct ccatgatcta tctgtttaac aatggtggtc
    23041 ccggaagcgt aggcggagga gctggttcta ctgatattct tatttcctgg atctataaac
    23101 ttacgactgg aacttctccg cagtattcta tggcttcagc aatcacgttg attatctccg
    23161 tcatcgttat ctgtgtgtca ttgctggtat ttaaaaagac aaatgcgttc aatatggggg
    23221 attaacaatg aaaaaatttc attcgagtgc tcgattccaa aaacgacttt ctcacttttt
    23281 tacttacttt tttcttttaa tactgtcagt cattatcatc tatccgcttt tgatcacagc
    23341 aagtactgcc ttcaagtcag gaaatattgc agcttttagt ttagatcttt caagtgactg
    23401 gactttagga aactttactc ggctattcag tgaaacactt tacgggactt ggtataaaaa
    23461 tacattgatc atttctgtcc taacaatgat cgtacaagtg actgtagtaa ctttggcggg
    23521 ctatacttat agtcgctatc gttttatcgg ccgcaaatca agtttgatgt tctttttagt
    23581 gattcagatg gtaccgacta tggcagcatt gactgccttt tatgtcatgg ctttcttatt
    23641 agaagcgctc gataagtact ggttcttgac aatgatctat ataggcggtg gaattccaat
    23701 gaatacttgg ctgatgaaag gatactttga cactgtacca atagatttag atgaatctgc
    23761 taaacttgat ggggcagggc atttcaggat ttttttccag attgtattac cgcttgtcaa
    23821 accgatgctc gccgttcaag cattatgggc ttttatggga ccttttgggg atttcatgtt
    23881 agctaagttc ctgcttcgaa cacctgaaaa tcagactgtt gctgtcggct tacaaacatt
    23941 tatttctgat gtgacaaacc aaagagtctc tctttttgct gctggggcaa ttttgattgc
    24001 actaccgatt tgtctattat tcttcttttt acaaaagaac tttgttaccg gcctgacatc
    24061 tggcggaaca aaaggatagg agcagagtat catgcaaaca tttccgttaa actatttttc
    24121 cgcattacgt acgccaactc aagtgtttgc gggacgcaaa ctattatcat ggcctaaatt
    24181 tttcttgata tttgtatttt tagtttcctt gatggtcatg cctgtgactc ttttttatgc
    24241 caatcagatc caagcgattc cattagaaca atttctatcc gttcattcgc tgatcgatga
    24301 acaaggaacc caaaaatttt ctgaacttga attatctgaa actgggttac aagcatcgca
    24361 acaaacgata gctgtgaccc ctgaaatttt ggtaggtgtg tctttatctg agaaacaaca
    24421 atccgatcat ggaactttta ttgattttga gaaggaacaa tgggtgatcc aacaaaagga
    24481 taaatctggt atacgccgct atacgatgaa ttatagccca tctttccaac cagattctgt
    24541 acgtacgccc gaagatttcc aacgattttt agaaagagaa ttttatgcca gtaatcgtcc
    24601 aacaatcatt ttatcttata gtttaagtct tggtcttgtc ctatttgtca tgacgagtct
    24661 cattttattc ggggcttcct ttttcttgtg gatgacaaga aaaagccaac tttcatccat
    24721 tcatacattc aaagagagtg caaatttgat gctgaatgtt atgggaatag gttccatggc
    24781 agcagcagtt gtagggttta tccactttga ctttatcttg atgctaagtg tccaaacaac
    24841 gataactgtc ttgctgcttt tatgggtttt tgtcaaaaca agattcaaag ataagcgtgt
    24901 cgaatgacaa gtttaagcga tttatgcgac aatgaatgtg aaaaaaatga aaaataaagt
    24961 agtttttgaa aaatttctga aaaaattaaa cttcttagtt gattcttttc ttttttagta
    25021 ttaacgtaat agggtagttt ttttaaggag gaaaatcatg ttagagaaaa ctttatacaa
    25081 tcaattattt tcacgttctt tctcgctccc tgtcgaagta aattattggg acggtacaac
    25141 gaaacaatac ggagatacgg acactcctcc ccaaatcaaa atcaatatcc atgaagaaat
    25201 cccaatgaaa gagattacaa gtaacgcttc tttggcacta ggagaagcct atatggatca
    25261 tcgaatcgag atcgagggaa gtatccaagc attgatcaac gatgcttatc agcaagcaaa
    25321 cagcttttta cgaggaaaag attatctaaa atggctgcca aagaaagaaa aacatacgaa
    25381 acaaaaaaac aaagaagata tccatgcgca ttatgactta ggtaacgact tttataaact
    25441 ttggctagat ccaactttga cttattcttg tgcttatttc aatacacctg aagatacgtt
    25501 ggaacaagca caaatcaata aagtccatca tatcttagac aaactcttta tcaaagaagg
    25561 cgaaacgttg cttgatatcg gttgtggctg gggaacattg atgttcactg ctaccaaaga
    25621 gtataatgtc aaggcaactg ggatcacact aagtgaagaa caatacgatt tgattaccaa
    25681 aagaataaaa gaagaacatt tagaagataa atgccgtgta ttattaatgg attaccgcga
    25741 actaaaaggc gaaacgtttg atcatatcac aagtgtaggt atgttcgaac atgtaggttc
    25801 tgaaaatctt gaaggttatt tcaaagtggt aaaagattta ttgaaaccaa aaggaacagc
    25861 tttgatccat ggaatcagcc gacaacaagg cggtgcgaca aatgcttgga tcaataaata
    25921 tattttccct ggcggttatg tccctggtgt tactgaatta gttgataata ttacaaaaaa
    25981 cgatttgcaa ctgattgatt tggaaagctt acgtcgtgac taccaattga cattagaaca
    26041 ttggacaaag aattttcatc aagtccaaga tcaagtcacg aaagaaatgg gtgattcctt
    26101 ctatcgaatg tgggatctat atttacaagc ttgtgcagct tcatttgaat caagtaatat
    26161 cgatgtgatt caatatttgc ttgttcaacc tggaaacaat gacatcccaa tgcatcgtgc
    26221 ataattgagg caaaagtact gtttaaagac tatttaaaaa gaagctaagt tttatgatgc
    26281 tccagaagtg aacttttttg tctaacttct aggattgtct ctaaagctta gtttcttttt
    26341 ctttatattt tcataataaa agtttggttc tttaattttt aaacatgaca gaaaattttt
    26401 tcttcgttat acttgtaata gaatactatt ttataacaaa ggggatcgaa tcaatgaaaa
    26461 aaactgttat ttatactagt ttatttgcag ctttgttttt agctgcttgt caatcaaata
    26521 ccaatcaagg cacatctggg aatacgtctt ccagtagcga acagcgctct tcacaggtat
    26581 cggactcctc aacaaaagca agcacatctt cgtctagcca gacaaaaagt tctagtgaag
    26641 caagtactca gtcagatatc aaagtgtctg cagctcgtgc gatcgaattg ttccaagata
    26701 aatacccaga tactgcaatt actagtttgg aattagatag tgattgggga tcttacttct
    26761 acaagataga aggtgtcgat gatcaaaaag aatacgaagc aagaattgat gctcaaaatg
    26821 aaaaaatgga gacagagaat ccagaaactc tagatcgaga tgaacaaaat ggtcaaaaga
    26881 aaaaagaaga tggtctggat gttgataaat tgatcacgat tgaggaagca gcaaaaatcg
    26941 ccgtcgacaa agtaggaagc ggtacggcaa cagattggga tttggataaa gatttaggta
    27001 ctacttattg ggaagtcaaa gtaaaagacg gcaatcaaag tacatcagtc aagatcaata
    27061 gccaaacagg cgaagtactg tcaactgaac aagatgatta agaatttctt cggaaaacaa
    27121 gagttgattt ttcttttagt catgatacac tagacaaaat aacttgtttg aaggagcgat
    27181 ttacttgagt ttagaaacga cctttgccaa tcatatcttt gccaatcctt tgatgaatgc
    27241 atctggtgtc cactgcatga ctacccagga attagatgaa ctggctcatt cagaagcagg
    27301 agcatttatc actaagagct gtacgatcaa tgaacgaaaa ggaaatcccg aaccacgcta
    27361 ttttgatgtt cctttaggga gtatcaattc tatgggactt ccaaacttag gtttttctta
    27421 ttatctagag tatgctcttg cgtatgaaaa agtgcaggaa aaccaaaatc aaccgttatt
    27481 tttttccatt gccgggatga gtgtccagga aaacttggaa atgcttgaga aaatagaaaa
    27541 aagtgggttt aatgggatca ctgaattaaa tttatcttgt cccaatgtgc ctggaaaacc
    27601 acaactagct tacgattttg aggccactta tgaaacactg aaagaagtat tcagcatctt
    27661 ttctaagccg ctaggaatca aattgcctcc ttattttgat tttgctcatt ttgatcagat
    27721 ggctgatatc ttgaatcagt ttccattgac ctatgtcaat gctatcaaca gtgtgggcaa
    27781 tggtttgtat atcgatacag aacaagaagc agtagtcatc aaaccaaaag aagggttcgg
    27841 cggcattggc ggagagtata tcaagccgac tgctttggcg aatgttcgcg ctttttatac
    27901 acgtttgaaa ccagaaatcc aaatcattgg tacaggtggg attcggacag gacaagatgc
    27961 atttgaacat ctgctatgtg gcgcaagcat gttacagatt ggtacagagc ttcataaaga
    28021 agggccggaa attttttcta gaatcataaa agaattaacg cagattatgt cagaaaaagg
    28081 atatacgtca attgacgaat ttaaaggcaa attacgcacc atttcatagc aaagagacga
    28141 aggtcatgat tgatcttcgt ctttttttat aaaaaacact cgtctgttca ttcctgtgca
    28201 agcgtactca ttgtatgtta gacgaacttg tgggaaaata acagaggcaa aaaggaggat
    28261 aatatgaatg atgcaacaga attagcaaaa gagcttaaag aaaaagaatt tagttttcaa
    28321 gaactagtta cggatataca gaaaaaaata aagaagcaaa atcatgaatt gaatgcgttt
    28381 gtcgcgcttg aaccattcga gagtaattcc cacgagctat tgattcccca gtcagaaaaa
    28441 gggccattgg ctggtatccc atttccttta aaaatgctcg gtcaagaaaa aaagggatgg
    28501 ctagctactt caggatctcg tttatttgaa acacaccgag cttctcatac atctaattat
    28561 gtgcggcaag cagaagcgat aggtctagtt ccttttggac aaaccaatgc gccagagttt
    28621 ggtttcaaaa atattacaga cccagtcatc tatggcccag caagaaaccc ttggaacctt
    28681 gaatgtacac cgggcggatc aagtggagga gctgctgctg cagtttcatc aggaatcgtt
    28741 cctttagctg gtgctagtga tggcggtggt tctattagaa tacccgcttc gttttgcgga
    28801 ttgattgggc tgaaaccttc gcgagggacg atgccagttg gtccatatgc ttggagagga
    28861 tggcaaggag ctgctattga tttcggcttg acagtttcta tgagagacac ggaggcttta
    28921 ttttatggga tgagaagtat ccattccggt gcgccatatc aagtatcgcc agttgagtgg
    28981 caaacacatc caagaaaaaa acggttgaag atagcttttt gtgttgattc accgatccac
    29041 tcacagatta gtgaagaggc agtacttgct gtaaaaaaag ctgtagattt ccttggaaaa
    29101 gaagggcatg atttgacaga gattccttac ccactagatg gtcgtaaact gatcacgtct
    29161 tattatcaga tgaatggcgc agaaacagca gcaatgatcc gctcgattga aacaggcatc
    29221 cagcgagctg tcagaaaaga agaacttgaa cccatttctt ggactttgtt ccaatatggt
    29281 cagaagctct ctgccgctac ttacatcgat tctctgcatg tatgggatga agcagcaatt
    29341 gtcatggaaa acctacatca agaatacgat ctattccttt caccaacgac tgcatttacc
    29401 gcccctaaaa tcagcgaaga tttgcaaagt gatgcgataa gagcacagat ggaacacatc
    29461 agtgattgtt ctgaagtaga aggtttagac gttgtttctt ccatgtttga ggagagcttg
    29521 aagatcacac cttatacaca gttggctaat ctgactggac aaccagcaat cagcttgcct
    29581 actcatttga caaaagaaaa catgcctcta ggaatccaat ttatggctgc cagaggaaga
    29641 gaagatttgc tatttcaaat cggctatcta tttgaagcaa atcatcagtt ccatttacca
    29701 aatccatgga aatagcagaa acaaggagaa gaagaatgaa gaaaaaaaga ttgtttgctc
    29761 taataactag tgcctttttt attttaagcg cttgcggtac agctgccgct aatcaggaag
    29821 ataacagctg gagtaatgta gaaaaaaaga aagaattagt agtagcgact tctggcacac
    29881 tgtttcccgc atcttattat aatgatcaaa atgaattagt tggttatgat gtagatttgg
    29941 caaaagaggt ggctaaacgg ttaaatgtca aaatctcctt taaagaatac aacgtggatg
    30001 gacaaatcac ttctgtttca aaaagagaat cagattttgc tgcgaacgat tttggtatca
    30061 caaaagaaag agaaaaaaaa ttctcccttt cagagacttt caaatattca tttgacagta
    30121 tgatcgtacg aaaaaaagat gattcaggaa tccactcact tgaagatctt aaagggaaaa
    30181 aagcagctgg cgaacccaat actagctata tgcgtttagc ggaaaaacac ggagctaaat
    30241 tagtcacata tgacaatgcc acgaatgatc agtatttaag cgatgtagca aatggtcgaa
    30301 cagatgttat tctaaatgat tattatctgc agaaaatggc cgtatctgct ttaccagata
    30361 tccctgtaaa aatactggag aacgtttatt tcaatgaaaa cgaaagtggt tttttattga
    30421 aagaaggaaa tgatgctctg aaaaagaaaa tcgatggcgt attaaaagag atgaaagaag
    30481 atgggacgac aaagaaaata tctgaaaaat attttcaaac tgatgtttca gtcaaaccga
    30541 aagaaacgat ggctgagtcg gtgagtgagt gatgtacttt cccctaattg atttttccct
    30601 catgtggact tcacttcctt tcgtactgca aggcttggta tacacattag ggattggctt
    30661 cgtgtcgttt cttttaggaa atttggtagg gttgcttttg acagttttag gtcttttgga
    30721 ttggctgcct ctgaatgtat ttattcgttt ttatttatcg ttctttagag gcattcccgc
    30781 actagtcctg ctgtttctgt tatatttcgg gttaccttat caattaagtg ccttaacggc
    30841 ttcagtcatc tgctttacga ttaccagcag tgcatttatt ggtgaaatct atcgaggatc
    30901 actagcaggc gtttcttcag gtcagtggga cgctggttta gcttctggtt tttctttctt
    30961 tggggtattg aaatatatcg ttttgccgca agcttttcga atctcagtac cggcactcag
    31021 taacgtagct atggatctag tgaaaggtac ttcgttagca gcaacaatca cagtgccaga
    31081 aatatttcaa aaagcaaaga tcataggcgg tcggacattc gattattttt ctctctatat
    31141 tttagttgca ctgatttatt gggtcatttg tatcgggatc gaaaaagtgc aacagtattt
    31201 agaaaaacgt tctatccatc agtcaatctg aaaaatagaa atggtgctag cgtggctggc
    31261 accatttcta ttttttgttt cttgtatatt ctgcctagta ttttaaaacc atttcatgta
    31321 aaatgtaaat cgaaaatatg ttcggaggta gaaaacatga agaccccgct aaccatcgaa
    31381 atagaatcct cgttatacca atattgcatc gagcaaggcg gcttggttgt agaagaagtc
    31441 acgatgcctg atgaccaagg aattgtagat acgcttagct gcttgacaaa atttgatgga
    31501 agccgagaat ggcgatgcta tgagctgaaa gtgacaaaag cagattttcg ctctacagcc
    31561 aaactgtcat ttgttggaca ttataattat tttgttttac caagtaaact atatgaagaa
    31621 gtaaaaactg aaatccctac aggaataggt gttttagtgt atcgtccgta cacggttgaa
    31681 gaggagatgc ctgcctctgg tacatttctg attgcgaaaa aagcagcaaa gaaagagctt
    31741 gctgtaccgg aagaagcttt gacaaaccgc ttcatggctt ctttattcag ggaagtaaga
    31801 aaagcaaagc aaatggagcg aggcgtaaaa tattttccaa aagatcaatt gtacaaagaa
    31861 ttgaaaagac gttcagaaaa tcaagatcca ttttctaacg aacgatatta tgaacggttt
    31921 ttagaggaaa tagctagtca ggcaatcaat gagttgcaag aagaagttat cgcgcttcag
    31981 catgattatg aatatttgag gcaacaacag cagatccgtc ggttgcctac agagccattg
    32041 gagtgaaaaa tatgtactat ccttatttaa gaggaaaaca atttgaccta ttagcattaa
    32101 aagaagcact gtcgcgagga ttgctctcca ataaaatcca accagtgatc gaaccagtac
    32161 gtgattccgc tacgcttaaa aatgtgatcg aattatttca aaaaaagcac catccaatcg
    32221 ctgtgatcca aaatccccaa gtaggacaat tcaaattatt cgatcagcat gtgcacagtt
    32281 ggacagtcaa agaaaatagc tctgtcgtcc ctgcacagat cttaacgcca gaaaatctca
    32341 gtgaagtact ggattcacct ccagcgtttc ttgttttcga tggacagcac tacccaaaag
    32401 atacagaggt gtggaaacag ttggcaggag ttgattcgaa atttttgata ccagatacta
    32461 gtcgctttcg catctggctg cccgaaaaca aaattgtgat ccgtgactct tttcaaacga
    32521 gaaagcatgt tgaaagttat gcagataaaa cagatgactt ttttagtgat gactatcttt
    32581 ttttccacgc agacggatat attggatttt ctgatttcac gatcgaaggt agccgttatt
    32641 tcgataaagg gttccctagc cgagcgcttg ctatccatct aacgtatatt gatgcttacg
    32701 gcaatatacg cgtgaaacat tttgtctctg acagtaacga ctctgctaaa gaccaagcat
    32761 tgaagtttct tgaagcagga gataaaatga aaaaatggat catgcgtcac catcatcaac
    32821 tcttgattac ttcaggaatg attgaattat tggctcttta ccagcaacaa aaattccctg
    32881 gactgggagt gttgaaaaag tggtcactta tgcatcattt ggaacttatt agtcacctgc
    32941 tagatcatcc aaaagattgg cttaaatatt attcaaaagt tcaggaacta tatgaagtga
    33001 tccaatgatt agataatgta aatgaaaaag gaatagaaaa gaggaaactg gatgtatatt
    33061 cggcaaacaa ggaaagaaga agtagaagaa gtggtagaaa tcattaatgc cgcagcggat
    33121 ttcattgctt cgcaaggctc tcctcaatgg caaaacgggc agaaaccaac aaaagaaacg
    33181 attttgaacg atattgaaag aaaagaaagc tatgtattaa tagtggaaga tcaaattgct
    33241 ggaacagctg cattagtctc aggcgtggat cccgtttaca caaaaataga tggtagttgg
    33301 aaagaatcaa ctggtcctta tttatcgatt catcgtgtcg ctttagcaga aagtactcga
    33361 ggaaaaaaac tagcaaaacc gttcttagaa catttgttga cgattgcagc cagcaaaaat
    33421 atccaagata tccgtatcga tacttatcca gctaatgaac cgatggaaaa gaccatctat
    33481 tctgctggat tcacttatca agggatgatt gaatttcctt ttgaacacgg cgaaagaaaa
    33541 gcatatcaaa taatcatcaa ttgactataa aatcatctga gcaaagataa agacattaac
    33601 actactgaaa aaaacaatca tatgatagcc aagaggttgt aacagaagtg gccagcttca
    33661 aaaaacaaga aagaatttcc gaaaatgctt ctcaaatttt tgtggaattt tagcgtattt
    33721 tacgaaatag ctgctttctg aaactccctt catcgagagg tcgtgacata gcttttgtca
    33781 cgatctctcg gcttatccta cgagtattat tcgatacatt tatatcaata atcctttttt
    33841 tattttatct gcagcttctt ggaacgctcc atcgtttgac acgactaaaa gtgaggggat
    33901 agctttcaaa cgtttcaact gtgctaaatc ttctacataa accgccagtc tggctgaaat
    33961 ctgttcagat gaatctccac gttttttcag gcgcgattgc aatgtttctt tagagatcgt
    34021 aataaagata ggacggacta ccgtcccgaa atactcatat aaatgccaaa aaccatttgc
    34081 tgtcaaaacg gcgtaacaat ttcctactgt cagtttcttt ttaatttctg ctttactgga
    34141 accatattta tgttgagcat attggtccca ctctgcaaac tcatttccct tgatcatctg
    34201 attgaatgta ttgtcagaaa caaaataata atcctcttga tctttttccc caggtcttgg
    34261 tgatcgattc gtgtaagtaa tgatcttttg ttccggctga aaaatagctt ctgcgagtga
    34321 agtttttcca gaaccagaag gaccgataaa tacatagcaa taattcattg tcatttctcc
    34381 tttgatgatt gaacaagtga aaaagctttg gaaaatattt ccaaagcttt taaaagtaac
    34441 tttgcttttt tgtattttat accagcaagc cagtcacaat agcacttaaa aagctgacta
    34501 aagtcgctgt tagcaaaatt ttcaatccgt gttttgcgac aagatttcct ttttcttcat
    34561 ttaatccctt catcgcacca gaaatgatcc cgatagaaga gaagttggca aatgaaacga
    34621 ggaagacaga tataattgct gttgtacgct cagtaaaatg gaaattacca tttgctaatt
    34681 ctgtcatagc aacaaattca tttgtaacca gtttagtcgc catgatacta ccggcatcca
    34741 ctgcttcttt ccatgggatt ccgctgatga aggcaagagg ggcaaagatg taacctagga
    34801 tctcttggaa agtgatgccg aatatcccgt ggaagatagc attgatcatg gcaatcaaag
    34861 caacaaaacc gataagcata gcagcaaccg tgatcgccac atggaaacca tcaagaatgt
    34921 attctcccaa catttggaaa aatgtctgtt ctttgttttc ttcaatgatc agctcatctt
    34981 ctttttcttc taatacatag ggatttacaa ttgaagccag aatataaccg ccaaataagt
    35041 tcaaaacaag ggcagtgatc acatatttag aatcgatcag tgccatataa gatccgacga
    35101 tcgacataga aactgtagac atagccgata cactcatggt gaacaaacgt ttctcagata
    35161 ggaagggtaa ttcctttttt atggaaataa aaacttccga ttgtcctaaa atagcagaag
    35221 ctactccatt ataagattcc aatttgccca tcccgttgat tttacttaat cctagaccaa
    35281 cagctttcat aaataatgga agaatcttga tatagcgtag aataccgata atagcagaaa
    35341 taaatacaat tggcatcaat acggaaagaa agaaaggtgc acttccttta tttgctacac
    35401 cggcaaaaac aaagtcaaca ccttgcccag caaaagcaag aagatgatca aacatttttg
    35461 ccaatacaga aacaaccgct gtaccaaaag ttgtacgtaa taatacgaaa ccaagaacaa
    35521 gctgtaaaac gaacatcaca attagtggtt tcaatttgat tgcttttttg ttactgctga
    35581 tcaaaaaagc aagccctaat gtgacaaata aaccaataat acctaataaa taactcaaat
    35641 aatcactcct attccaaatt ccgttttatt ttacctgttt tgcaaataaa atactaatct
    35701 ttagtgaaat tttcattact tttttagagt gaaacgcatt cattttccat tagaaaataa
    35761 agcgaacaaa ataaaaggtt taaaaagtgt aacgtcttag taaagtctat aaaaaaacga
    35821 aactatcgat taagctaata gtttcgtttt caacatttat aagtaaactt tttctattgg
    35881 taatatagat tttcggaagg gtagacaggt gcgcaggtaa tatcgattcc tagttttttc
    35941 aatgtttgtt catcattttt attgaccatc acagtagagt gggcttgtgc tccctcaagt
    36001 tcagctagtt tatcataagc aacttgagcg attggattcg ttactgcact gattgataaa
    36061 gcgatcaaga cttcattgat gcttagtgca tccaaagagg aatgaagtgt ctttgatttc
    36121 aatccttgta ttgtttcaag aactaatggt gacaataaag gaatgtcatc tgcgatatgg
    36181 gctaaatgtt ttactgcatt taagataact gccgctgacg catccatcaa tgaacttgta
    36241 cgcccggtaa tgatgacttc atctggtaat tcgattgcaa tcacagcaga aggatctttt
    36301 gtaggacttt gcgcttgaat cttatctgca tagtcatggg cagggactac cacgtttcgg
    36361 tcttcaggac gtaaaccgac ttcttccata atcagtttga ttcgattaac tgcttcttcg
    36421 tcgatcaatc cttttttgaa gtcgcattct gtttggaaat aacgacggat gatttcttgt
    36481 ttagaggctt cttggacaac ggtatcatcc acgatcccaa atcctacacg attcacaccc
    36541 atgtcagtag gagattgata aacagattct ttaccagtaa ttttttcaat aatacgtttg
    36601 attacaggaa atgtctcaac atcccgatta tagttcacag ctactttatt gtaggcatca
    36661 aagtggaagg aatcgatcat attgacatct tttagatcga ctgtggctgc ttcataagca
    36721 atgttcagcg gatgtttcaa cggaacattc catactggga aagtttcgaa cttggagtaa
    36781 ccagctgcat gtcctttttg gctttcatga tacagttggt tcaagcaagt agcgagcttt
    36841 ccacttcctg ggccaggagc tgtcactaca acgattggtt tagttgtttc aatatactca
    36901 ttttttccaa aaccatcttc tgagacgatt ttttctacgt tgactggata gtcttcgatt
    36961 tctgagtgtt tatagacctt gatgtttcgt ctttctagtt tattgataaa gactttcgtg
    37021 gcaggctgtc cactatagcg agtgataacg acactgttga ttaataagcc ataaccacgt
    37081 aattcatcga ttaaccgcaa gatatccata tcataagtaa tcccataatc accacggatc
    37141 ttattacgtt caatatctcc agcatagacg caaatcagta tttcagcttg atctttcaat
    37201 ttttgcagca atttgatttt cgcatcttct tcaaatcctg gaagtacacg tttcgcatgc
    37261 ttgtcgtcaa ctaacttccc accaaattct aaatataatt tgtcatagtg gttcactcgt
    37321 tccaaaatat acgctgactg ctcttcaata tatttttctg aatcaaaacc aatttttttc
    37381 actaggttcc ctccttaagt actaaagcat tttttcattt ttttgaggat tcgtcaactg
    37441 gaagagcgaa attcctcaaa aaaacaaaga aaatggttta aaccaacttg tctagatagt
    37501 tattcattgg tatgatagtt tttgataagg aaggtgctac atgaaaaaaa gacagaaaaa
    37561 gaaaaacgca tataaacact atatccgttc gatttttacc ggttatgaaa gaatgcttga
    37621 agatcccgaa ttggaacaat tgacgtttac ttatctaaac gaagaaacgc agctaactcg
    37681 tgatgagcat cagcgcattc atttcacaac aagagatctg cctagtaaat aaaaaataag
    37741 ataaaggtca tcggattcga ttctgtccta tcagatggaa agactagttg atcgaaaaaa
    37801 aggctgtacc aaattttggt gcagcctgtt ttatggagca ttgcccgctc ttttttataa
    37861 caatttttta cagaaaatgg tactattgaa gtattaataa gtgaaaaaca gatgagtaag
    37921 aggaggcaag tagtatgaaa gagccagata caaatgcaaa ttatgagcca gacgaactag
    37981 aggagacgaa cgcggatcag gatacaggac aagaatcgtc ttctttttac gatatcaaag
    38041 aaaaagcaag catgaatctt tcccaattat tagaagactt acaggctttt gaaaaagctg
    38101 tccgcgaaga agatatccct gagatctacc gtttatacaa tggccgattg aatgaagaat
    38161 taaaaagaag ctcaaatgaa aatcacgaga tcgatcaatt gctgatgcgt aagatccatg
    38221 atcgtttttt acaggaattt ccttttatgg aacaagtaga aaacatctcg ccaacgatga
    38281 gctattacaa aatcggcact tattaccatg atcggcccac ggtcggtatt gatgcaagtc
    38341 tgccagaaat ttttgttttg cctaagatcg atgaagagtg ggagaaatat tcccaaccgg
    38401 agtcaacgga ctatgacaaa aaaatcaatg agctagacgc aaaggtgatc actgctcaga
    38461 cagaaattga acgtttagat gaacaaataa aagaaatcaa tcgacaaatg actgatttga
    38521 acgacaataa aggattcttg aatcgtaaaa aaatcgagga agaaattcaa gaattagaga
    38581 agaagaagca agtactatct aatgaaaaat taggctggct tccttatatt gagacgcctg
    38641 aaacaatcca gcagcaaaaa gaagcattga agcaagaagc aagagcagac cagcttcgtg
    38701 cagccatcgt agaaaaagaa caaaggcaaa tcagacgcta tttcggtagc aaagagggat
    38761 tcggtcaagc aatccatgaa ttcttgatga attatcttgg caatgagaat cctaataatc
    38821 aatctaatga aggaggttcc gagtatgaat gaacacgatg aagcagaatg gacaaaaaat
    38881 gaagaacaaa cagaagatca tgaagaaacg aacacttcat tggaagaaca aacaagtaat
    38941 cctctggatg acacagaaga aatccctatg ctagaggaaa caaaaataca tgaaaaagaa
    39001 aaagtaactc cgacgaatac gaatactgca gaaaacaccc atgcttacga aataactgaa
    39061 aatcccgggt atttagaagg aaaagagcaa gatcatcaag atatcgtcag tttgattgaa
    39121 acagcggaag cagaattagc ggatttacgt atgagaaaag atttgatcga atcagatttt
    39181 ccacaattat tagaatcata tgatcgtttt atattgaatg aagaaccaat ctccacgatc
    39241 ggaaagacaa gcctgatcga atacttcacg tatgaattac tgaaaccgtt gaatgatatt
    39301 ggtctggaac ggtcaaattc agcttatgac acatggaaaa caagacattt ttcgatcaat
    39361 tatacgttga atggacaaaa cgaattagtc ttttcctttg tattaccgac catcgatcaa
    39421 cgataccaag tggaatcgat gagtctccta aacgtatcac cagagacaat ggaaatcaat
    39481 gtccaagatg accgggtgct ttctttgatc cgacactggt cagtggatcg agtattctct
    39541 gcagggcaga tcacgatatt caatcacaaa ttgaatcaat tattagccca tgcaagaaca
    39601 cttggtttta ccgttaatca gacattacta gacaatacga agccacttcg tttgaatctt
    39661 cagagcgaat ttgaattgac gaatgaagta ttggatgata tttttatcgt ggcaatgaaa
    39721 aatccatcct acgatatgga aaagatcaac gaaactactt atcaagtacg attagataaa
    39781 ggacagtctt taacgataac aacaaacgaa aaagatcaaa cagtcttgtc gatctcatct
    39841 ggagactacc cacgatctgt cattgatttc tttatcaatt acgaattctt ggtcccatta
    39901 atggtgagaa agtcctgatt ggataagttg gattaccaga tgtcattcag ataattctgt
    39961 tccaaaggac ttgcggcagt tatccagaat aattgtcaca agtcttttct gtatgtacta
    40021 aatggacgtg aataacttgc tgatgaagga gggatttcat taggaatgtc cacaaataaa
    40081 atagcacagc ttagcttatt gtctgctgct tgtgtagcag gtaggatcgc ttttcaattt
    40141 attccgaatt tccagccaat gactgctatt tttttattta tagtacttta cttaagctta
    40201 aaagatgcgt taattgtcat gagtctttct attgcgatca gcagttttta ttttggtgtt
    40261 ggaccatggg tcatcggcca atggatcagc tatactgttg ttctgagttt gttttcaatc
    40321 gtctgcttga gaaaaacagt gcaaaggaat ctatggttaa agggagtctg tttttttctg
    40381 gcgggcatgg tttacggaat aggtatgacc gtttttgata cgctgttata tcgtttgcct
    40441 caaccttgga tctattacct tcaaggggtg tcgtttgatt tgatgcacag tataggcaat
    40501 gttgtgttct ttcttgtttt tctgccagtc gtcaaacgtt tttacaatca ttcaggagga
    40561 aaaaatgaaa aaaataattt atagtttgct tgcaatgagt gtcagtatac tcattttgac
    40621 aagttgcagt acaacctata cacaatcggc ttccaaccaa aaggaggaag taaaaacatc
    40681 gcaaaaagca acaatcacgt tacaagaaaa tggtcaaaac ttttcggaaa aagaagtggt
    40741 cttcaaaaaa ggcgatgatt tactgagtat tttgaaacga aattttgaag tcaaagaaga
    40801 taacggcttc atcacttcgc ttgaagggca ctcacaggat gagaaaaatc aactctattg
    40861 gatgtttact gtggacggga aaagtgctac tactggtgcc aaagagatca aattgaatga
    40921 tggacaatcg gtcattttca atttgtctaa actataagtt tcataaaaaa ctgttaaagg
    40981 gcgaatattg ccataattca gtgagttcag ttgatgaaag aaggaaaata acatggaaaa
    41041 ttttaaaaaa agcggaatgt tttccgtaat tgctgctcta ggggctaata ttctagttgc
    41101 tatcagcaaa tttgtcggct atgcaatcag tggcagtgcc gccatgctaa acgaaagcat
    41161 ccacagtatc gttgattgca gcaatcagat tttgctgtta ttcggtgata aacaagccaa
    41221 taaaggacag agcgagttgc atcaatttgg tgaaggacga gcaaaatact ttttcagtac
    41281 aatcgtcgca atgatgcttt tcttcggcgg tggggcactt ggggtgatgg aagcttttga
    41341 aaagttgacg catccatccc atgaagtggg taatccgatc attgttatag caatactgtt
    41401 atttggtttg gtaatcgaag gaagttcgtt acgcgtggca atgaaagaaa tcaaagaatt
    41461 gaatactgaa aaactttcca cgatgcgctt tttgcgcgaa agtcgacaca gtgaaatact
    41521 gattattttt acggaagact tgtgtgctgt aatcggtttg gtcttggctt tagcaggaac
    41581 aatattgact tatgcaaccg ggattgctgc atttgatgcg atcagcggat tgctgatcgg
    41641 tttcttgctg atgggagccg ccattttcct agcaaaagaa ttttatagtc tgatcatcgg
    41701 tgaaagcgtg acagcaagcg atttatcaaa aatcaagtta gcttttgaac gtcctgagat
    41761 cagtcggctg atcgatataa aaacagttca tttaagtcca acggagattt tagttgcagc
    41821 aaaagcagat atcgttggta acaaagagaa tgaagcttac tcggtcatca atgatattga
    41881 aaaaaatatg cgtcagtctc ttcctgacaa gaagatgtat atatacattg aaacagataa
    41941 atatgaccca aattattctc gtaaataaaa cgtttattca tgaattgaag caaaaatgag
    42001 aaaaatataa tcttgttttt aaataaaacg gctacaattt agcgaattta tgttacaatg
    42061 agtccgctgt ttatttgttg taattaaagg agatatttta atgaacaaaa agaaaagtgc
    42121 gccatggctt caagctttgc atgcagtgat gccactttgt ataagctata taccagtagg
    42181 attagcttgc ggagtattgt tgcaaaaagt tggatttgat cctctcttat caggactgtt
    42241 atcgattctg gtcttttccg gtggcgcgca gtttttagtt gcatctatgc ttacgacgca
    42301 agcatcattt gcaacgacat tattgatggt tttcttttta gaattacgct atacattatt
    42361 aggagcaagt cttgccggtt ttatgaaaaa agaaaagcgg acgtttttgg ctgtcttttc
    42421 tcaatcactg aatgatgaaa actatgcggt gaactactta aagttttcaa ctgacccttc
    42481 ctggaataaa agaaaggctt tatatgtcaa ttggttctca atgactgcat gggccggtag
    42541 caatatgatc ggtaattttt tcggctcggt gattcgagta gatgctgacc ttgtccattt
    42601 tgctttgacg gccatgttca ttttcatgtt tatcatgcag atgaaaagcg cactactgat
    42661 ctttacaggt ttattctcag gcgtattagg ggtcgtattc atggtattat tccaaaatac
    42721 tttcggattg attgcagcaa cagtcattgc ttcatttgcc ggatttatgc tggagaaggc
    42781 attcaaaaaa gaaaaagcta agaaactaag agaccgcggt aaagatgtca atgagccgct
    42841 cttcaacttc ccggaacagg aggccaatca ccatgactaa actatatttg ctacttttag
    42901 ttgcttgtct gttcatcgtg gcatatatcc cgcgcttgtt cccgatgctt tattttactc
    42961 atcgaaaagt gccatcctgg ttcagtgact ggatgaaata tgtaccggtg gcattatttg
    43021 cggcgttagc ttttaaagat gttttcatta ctcatgaaca tctggatatt gcttggaata
    43081 tcaaaatagc tgcaatgatt ctagtagcag gcgtggctta caaaacacgt tcgatggcgt
    43141 tgtcagtttt gacaggactt gcgtctgtct ttttgctgtc tatgctttaa actgttttat
    43201 tttattaaaa acagagattg cgacataagt tgttcaaact gatctatccg aactgcattt
    43261 gcaacagtca attctggatg caagtttatt cgtagggata aaaaagatta gtgttggctt
    43321 atgatgcaag ctctttttta ttttaggaat tagagaggga tttgtccttc tatgattttt
    43381 tgctataata gggaagatgt ttattttatg aaagaatagt tgagggaatt taaatgaata
    43441 aaaaattgat gcaaggaaca ttttggctaa cctttgctaa tcttctatgt aaagttttag
    43501 gggtagtcta tttgattccc tggctaagta tgatggggaa caatcaagat ggtatgcttg
    43561 ctacgacatt gtataatgtt ggctatcttc cttatggatt gttcctgatg cttggtacag
    43621 tcggatttcc aaatgcgatt gcaaaaaaag tagctgtagc cacaaaagaa ggagataacg
    43681 ctgcttgtcg gctgattttt agaagtacga tcaatatcat gtttgtcatc ggtatcgcct
    43741 ctgctgcact gatgtattta tttgctcctt tactagccgg aatcagtccg attgctaacg
    43801 ttaataatgg gatactagct atcagaagtt tatgcccatc tttgattgct atcccaattc
    43861 ttagtgcaat gagaggatat ttccaaggaa aaaatgatct gcggccttat ggaacttctt
    43921 tgattatcga acaggctgtg cgagtgatcg ttatcttggc tggtacttat tacttgcgcg
    43981 tattgactga cggaaccata ttagaagctg tattgatcag tacagtcgct tctttcttcg
    44041 gtgggttagc tgcgattatc catatgtata tcgtcggacg cagaaaagac ttcttcgaac
    44101 ttaaagattt tttgatttcc agccgttatt tcgaaagaga aaaccgatct gcctctgtct
    44161 ctatcattcg ggaaacacta ccgtttattt ttgtcggctc agtcattact atcgttcaaa
    44221 tgatcgacca agtaacgatg aaaccactgc ttcacttttt ccgtccagag atcgcagatc
    44281 agcaactgga attcttattc agccgtgctt ctgtcaatcc aaataaattg acattgatcc
    44341 tgatctccat ggtaggaaca gtagctataa gcagcttgcc aatattaagc acgatgaaga
    44401 aaaaagatcg gatacaaatc gaaaaaacag taggagatag tttttcgatt gcgttattga
    44461 ttcttcttcc ttcattgact ggaatgtctt tattagctgg accactatac acattgtttt
    44521 ttggctatga tcctgaaagt gtcggatatt tccaaatggc tttgctagct tctttgtttt
    44581 tctctttatt cacgatccta tctacgatgc ttcaatcttt gaatcaccat cgttttgcga
    44641 tccgattaac tgtagaagca attatcctga aagtcatctt ccaagtaatc ggattaggat
    44701 tagttggcgg atacgggatg agcttatcaa atggcttagc attcggtatt gtctttgttc
    44761 gcggctatca ctttatgtgc aaagaatacc gaatagcacc tttagcaaag atcagcaatt
    44821 ttttcttaaa aactttccgc tccaccctga ttatgcttgc agtatgtttt gccgttttct
    44881 ttttgctgaa tcagcgattg tcaatggaat caaaaagcta tgcaatgatt tattgtgtgg
    44941 ctgtaggaag tattggcggc ttagtatttt tattttctca attcggaaaa aatgggtttc
    45001 gaatgttgaa aaatgttaaa catcatagca gaagcaaaga ataggttaac taatcgtttt
    45061 ttgctgtcac ttgaaaaacg gcaactcgta tgattacgct tgtcgttttt ctattttcca
    45121 attttagttt catatctaat cttacttatg atatagttat aaggtctgga aaaattcttt
    45181 gaaacgagga aattaaacac atgaaacttg cagtagttac agatagttcg gcatatctgc
    45241 cggaacgtat caagaaccat cccgacttat tcgttattcc aatccctttg attatggatg
    45301 ggaaaattta taacgaagga atcgatatcg aagcggatga atattatgga cttttaaaaa
    45361 acagtaaaga ttttcctact acttcacaac ctgtcgtagg ggaagttttg ggtttatatg
    45421 aggaattgaa agagaaaggg tatgacacga ttttaagtat ccatctttct tcagggattt
    45481 ctggctttgt caacacatta tttgcgatga aagatgacat cagcggagta acggtcattc
    45541 cttatgactc taagatcact agtatgccga tgggacacat ggtagaagct gccttagact
    45601 taaatgatga aggtcagtcc cttgatgaaa tacttgcaca tatcgatcga atccgagata
    45661 atacctatgc ttatctgatt gtagatgatt tgaacaattt agtacgagga ggacgtttga
    45721 caaatggtgc agcgctgatt ggcagcttat taaaaatcaa accgatcctg acattttctg
    45781 aagggaaaat tgttttattc gaaaaaatcc gttcaagtaa aaaagcattt gctcgagctg
    45841 aagaaattat tggtagaaga aataacgaaa tacaaccgcc agtcaaactt tacgtgatac
    45901 atgcaaataa tttagaagta gcgattgcgg agaaagagaa attgcagaaa aaatatccaa
    45961 atgcagaaat cgaaattggt catttcggac cagtcattgg tacacattta ggtgaaaaag
    46021 ccatcggcat tgccatttca gcgcaataat tccttttatt caaaaaacat accaaaaata
    46081 t
//
LOCUS       DAMYXM010000016        45015 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.016, whole genome shotgun sequence.
ACCESSION   DAMYXM010000016 DAMYXM010000000
VERSION     DAMYXM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.016"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            212..604
                     /locus_tag="PNS21_001044"
     CDS             212..604
                     /locus_tag="PNS21_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDI5158765.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="PNS21_001045"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="PNS21_001045"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDI5158766.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="PNS21_001046"
     CDS             3165..4775
                     /locus_tag="PNS21_001046"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDI5158767.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="PNS21_001047"
     CDS             5067..5936
                     /locus_tag="PNS21_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDI5158768.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="PNS21_001048"
     CDS             6101..7372
                     /locus_tag="PNS21_001048"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDI5158769.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="PNS21_001049"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="PNS21_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDI5158770.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="PNS21_001050"
     CDS             8763..9026
                     /locus_tag="PNS21_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDI5158771.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="PNS21_001051"
     CDS             9471..9920
                     /locus_tag="PNS21_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI5158772.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="PNS21_001052"
     CDS             9977..11395
                     /locus_tag="PNS21_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDI5158773.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="PNS21_001053"
     CDS             11395..12255
                     /locus_tag="PNS21_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDI5158774.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="PNS21_001054"
     CDS             12429..13202
                     /locus_tag="PNS21_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDI5158775.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="PNS21_001055"
     CDS             13349..13714
                     /locus_tag="PNS21_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HDI5158776.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="PNS21_001056"
     CDS             complement(13862..15070)
                     /locus_tag="PNS21_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5158777.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="PNS21_001057"
     CDS             complement(15089..15367)
                     /locus_tag="PNS21_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158778.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="PNS21_001058"
     CDS             15521..16216
                     /locus_tag="PNS21_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDI5158779.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="PNS21_001059"
     CDS             complement(16658..17455)
                     /locus_tag="PNS21_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDI5158780.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="PNS21_001060"
     CDS             complement(17452..18636)
                     /locus_tag="PNS21_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDI5158781.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="PNS21_001061"
     CDS             complement(18661..19908)
                     /locus_tag="PNS21_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDI5158782.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="PNS21_001062"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="PNS21_001062"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDI5158783.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="PNS21_001063"
     CDS             21888..22202
                     /locus_tag="PNS21_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDI5158784.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="PNS21_001064"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="PNS21_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDI5158785.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="PNS21_001065"
     CDS             22887..23531
                     /locus_tag="PNS21_001065"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDI5158786.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="PNS21_001066"
     CDS             23546..23875
                     /locus_tag="PNS21_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDI5158787.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="PNS21_001067"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="PNS21_001067"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDI5158788.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="PNS21_001068"
     CDS             24863..25687
                     /locus_tag="PNS21_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDI5158789.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="PNS21_001069"
     CDS             25680..26027
                     /locus_tag="PNS21_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDI5158790.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="PNS21_001070"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="PNS21_001070"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDI5158791.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="PNS21_001071"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="PNS21_001071"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDI5158792.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="PNS21_001072"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="PNS21_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDI5158793.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="PNS21_001073"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="PNS21_001073"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDI5158794.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="PNS21_001074"
     CDS             31657..32451
                     /locus_tag="PNS21_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5158795.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="PNS21_001075"
     CDS             32441..34483
                     /locus_tag="PNS21_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5158796.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="PNS21_001076"
     CDS             34764..34913
                     /locus_tag="PNS21_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDI5158797.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="PNS21_001077"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="PNS21_001077"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDI5158798.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="PNS21_001078"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="PNS21_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDI5158799.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="PNS21_001079"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="PNS21_001079"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDI5158800.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="PNS21_001080"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="PNS21_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDI5158801.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="PNS21_001081"
     CDS             39330..40412
                     /locus_tag="PNS21_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDI5158802.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="PNS21_001082"
     CDS             40412..41215
                     /locus_tag="PNS21_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDI5158803.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="PNS21_001083"
     CDS             41285..42025
                     /locus_tag="PNS21_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDI5158804.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="PNS21_001084"
     CDS             42036..43040
                     /locus_tag="PNS21_001084"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDI5158805.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="PNS21_001085"
     CDS             complement(43085..44929)
                     /locus_tag="PNS21_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158806.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14485 a         7842 c         9358 g        13330 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    45001 aaaaaatgag ataaa
//
LOCUS       DAMYXM010000017        40658 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.017, whole genome shotgun sequence.
ACCESSION   DAMYXM010000017 DAMYXM010000000
VERSION     DAMYXM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.017"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(73..639)
                     /locus_tag="PNS21_001086"
     CDS             complement(73..639)
                     /locus_tag="PNS21_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDI5158807.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(667..2169)
                     /gene="eat(A)"
                     /locus_tag="PNS21_001087"
     CDS             complement(667..2169)
                     /gene="eat(A)"
                     /locus_tag="PNS21_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDI5158808.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(2427..3548)
                     /locus_tag="PNS21_001088"
     CDS             complement(2427..3548)
                     /locus_tag="PNS21_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDI5158809.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3674..4615
                     /locus_tag="PNS21_001089"
     CDS             3674..4615
                     /locus_tag="PNS21_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDI5158810.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(4765..5661)
                     /locus_tag="PNS21_001090"
     CDS             complement(4765..5661)
                     /locus_tag="PNS21_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDI5158811.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            5930..7270
                     /locus_tag="PNS21_001091"
     CDS             5930..7270
                     /locus_tag="PNS21_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI5158812.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            7488..7931
                     /locus_tag="PNS21_001092"
     CDS             7488..7931
                     /locus_tag="PNS21_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDI5158813.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(8435..9853)
                     /gene="nhaC"
                     /locus_tag="PNS21_001093"
     CDS             complement(8435..9853)
                     /gene="nhaC"
                     /locus_tag="PNS21_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDI5158814.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(10256..11479)
                     /locus_tag="PNS21_001094"
     CDS             complement(10256..11479)
                     /locus_tag="PNS21_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDI5158815.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(11510..12349)
                     /locus_tag="PNS21_001095"
     CDS             complement(11510..12349)
                     /locus_tag="PNS21_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDI5158816.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(12366..13046)
                     /locus_tag="PNS21_001096"
     CDS             complement(12366..13046)
                     /locus_tag="PNS21_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5158817.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(13048..14121)
                     /locus_tag="PNS21_001097"
     CDS             complement(13048..14121)
                     /locus_tag="PNS21_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI5158818.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(14177..14415)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(14458..14691)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14768..15439)
                     /locus_tag="PNS21_001098"
     CDS             complement(14768..15439)
                     /locus_tag="PNS21_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDI5158819.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(15526..18204)
                     /gene="mgtA"
                     /locus_tag="PNS21_001099"
     CDS             complement(15526..18204)
                     /gene="mgtA"
                     /locus_tag="PNS21_001099"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDI5158820.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTRFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            18863..19300
                     /gene="ndk"
                     /locus_tag="PNS21_001100"
     CDS             18863..19300
                     /gene="ndk"
                     /locus_tag="PNS21_001100"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDI5158821.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(19322..20188)
                     /locus_tag="PNS21_001101"
     CDS             complement(19322..20188)
                     /locus_tag="PNS21_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI5158822.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(20348..22441)
                     /locus_tag="PNS21_001102"
     CDS             complement(20348..22441)
                     /locus_tag="PNS21_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDI5158823.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(23190..23813)
                     /locus_tag="PNS21_001103"
     CDS             complement(23190..23813)
                     /locus_tag="PNS21_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158824.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(23972..25369)
                     /locus_tag="PNS21_001104"
     CDS             complement(23972..25369)
                     /locus_tag="PNS21_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDI5158825.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(25401..26372)
                     /locus_tag="PNS21_001105"
     CDS             complement(25401..26372)
                     /locus_tag="PNS21_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI5158826.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            26746..27171
                     /locus_tag="PNS21_001106"
     CDS             26746..27171
                     /locus_tag="PNS21_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158827.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(27659..28360)
                     /locus_tag="PNS21_001107"
     CDS             complement(27659..28360)
                     /locus_tag="PNS21_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDI5158828.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(28376..28816)
                     /locus_tag="PNS21_001108"
     CDS             complement(28376..28816)
                     /locus_tag="PNS21_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDI5158829.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(28816..29391)
                     /locus_tag="PNS21_001109"
     CDS             complement(28816..29391)
                     /locus_tag="PNS21_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDI5158830.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(30239..30718)
                     /locus_tag="PNS21_001110"
                     /pseudo
     CDS             complement(30239..30718)
                     /locus_tag="PNS21_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(30877..31578)
                     /locus_tag="PNS21_001111"
     CDS             complement(30877..31578)
                     /locus_tag="PNS21_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI5158831.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(31686..33095)
                     /locus_tag="PNS21_001112"
     CDS             complement(31686..33095)
                     /locus_tag="PNS21_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDI5158832.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(33143..35008)
                     /locus_tag="PNS21_001113"
     CDS             complement(33143..35008)
                     /locus_tag="PNS21_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDI5158833.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(35129..35980)
                     /locus_tag="PNS21_001114"
     CDS             complement(35129..35980)
                     /locus_tag="PNS21_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDI5158834.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(36290..37003)
                     /locus_tag="PNS21_001115"
     CDS             complement(36290..37003)
                     /locus_tag="PNS21_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDI5158835.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(37071..37397)
                     /locus_tag="PNS21_001116"
     CDS             complement(37071..37397)
                     /locus_tag="PNS21_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDI5158836.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(37629..38846)
                     /locus_tag="PNS21_001117"
     CDS             complement(37629..38846)
                     /locus_tag="PNS21_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI5158837.1"
                     /translation="MSEKRRDNRGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            complement(38928..39131)
                     /locus_tag="PNS21_001118"
     CDS             complement(38928..39131)
                     /locus_tag="PNS21_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDI5158838.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            complement(39592..39822)
                     /locus_tag="PNS21_001119"
     CDS             complement(39592..39822)
                     /locus_tag="PNS21_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5158839.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            complement(39819..40241)
                     /locus_tag="PNS21_001120"
     CDS             complement(39819..40241)
                     /locus_tag="PNS21_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDI5158840.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
BASE COUNT        11599 a         8539 c         6940 g        13580 t
ORIGIN      
        1 taacctaaaa aaggcactgc taagcaaaag cagtgccttg aaatatttta tttaaataac
       61 acaaaaacat aattactcat aagaaaccaa ttctgtatta tctaccatga atgtaaagaa
      121 cccctcatta ggcatcggat gaaaataagc attgatcact cttatcaagg cattatggca
      181 aactaacaga tagacatttt cttcgtcttc gatgcattct ttcaataaag gaacgatccg
      241 tgcatacaca tctaagactg actccccatt tgggaatcgt acagcgaacg ctaaacgtgc
      301 tttttgaaag ttctcatctt tgcttggcag tccatcataa tccccaaaat ccatttctac
      361 caatcgttca tcaacggtca gcggcaagct gagacgatca gcaactgctt gtgctgtatc
      421 tcttgctcgt tgaagagggg aatggatgat tttcgtgatt ggaacaggca aatctactac
      481 cttttctgct agtttctgtg cctgcatctg tcctttttca gtcagcggca catctgcccg
      541 tccgcaaact aatccttgtg cgttccaagt tgtttctccg tgtctcgtta cataaagcat
      601 gtgattctct ccttcgttgg caaagttccc cttaaccata ccttgttgtt cttgagatta
      661 caagggttag attctctcaa gagaaataat ctcagtagat actttatcca ggaaggtttg
      721 atcatgttcc actagtaaca tggcaggttt cacgttcaag atcagttgtt ctaattgttc
      781 ttgattgaag acatccaaat aattcaatgg ttcatcccat gtatatagtt cagctggctg
      841 tgataaagat ttagccaatt ccactttttt ccgttggccc atactcatct gctcgatctt
      901 gttatgaaaa acatctcttt ccatcccaag ctttcggagg ttgttcaaaa atgcttggta
      961 gtctacttgg tttttctctg caaattccgc caacgttcct cgattgtctt cataattttg
     1021 gcttgcataa ctaatgctca gatgttttgg ctggtatttt tctcctataa ccttgccgtt
     1081 gaatgccccc agaagataat ggataatgga tgactttcct gcaccgttag gacctgaaat
     1141 cgccacccgc tgatgaggct cgattgtaaa atgaattggc tcgaataaca gattttcata
     1201 ccctaattga agatcttcta cgcttaaaag tcgcttatgg tgagacgctt ggctattcat
     1261 cgtcaaagaa tcgatatatt cgatatcttt taatagtttt tctttctcgc tgatctgggt
     1321 ctccatccga ttcacgattg ctttggatcg tttcatcgtc cgtgcagcat cagcacccac
     1381 tgctccttta ttcactcgtc tagattcagt atcgatgaat ccgacttgtt tctttgtttt
     1441 atctccttct cgggaacgag accattcggc tttttcagct gctgttttct ttagcctact
     1501 gacttctttt ttcaattttt cattttgagc catttcaaat tcatcacgaa gttttttctg
     1561 ttcttcatag atagagaaat tcccttgata aagttccagt tgactttttt cgattgctaa
     1621 aacatggtcc actacttcat cgataaatcc ccggtcatgg ctgaccacga tgaagccttg
     1681 ttttttcttt ttcaaataag ccgctacttg ttttctacca gagatatcca aatgattcgt
     1741 tggttcatcg attaacggga aatgagtgtc atccacaaat aaaagtgcca gtaggacttt
     1801 tgtcttctct ccccccgata gtgtgctgaa ttctctccat aagatttcag gatctgtttg
     1861 catcaattgg agctctcttt cgatttccca tatctcaaaa tccgtaatat catttaacac
     1921 gtaataggtt aaacgttctt tatcttttgt ctgttgcggg aaataggcaa attcttgctg
     1981 atggattact tgcccttgat aaggtagttt gttttgtaga atattcagta aagttgtctt
     2041 tcctcgaccg tttcgtccga taagtcctag tttccattgt gtgtcaaaat ttagatttgc
     2101 ttgttcaaat aataatgtgc cttggctgtc gtagccgaat gtcagatttt ttatttcgat
     2161 tttagacatt ttccatcacc tcgtaaaaaa agaccagtcc tattttcaga actggctaaa
     2221 gaggatgcaa gggttggata cacacaaaaa gacccataca tcgaaaaaac caagccaatc
     2281 ccgaaaatct gcacactgct tccttgtctc cacaaggtac agtctgaatg cagctttcat
     2341 caatcgactt aatttttcaa tgcacggatc cctcactttg tcctatattt gaaattacga
     2401 tagcataaag aaaatgattt tgtcaactac ttcgggagga tggtgaaaaa tctgctaagt
     2461 gtctgtccta agatttttgt tttttctaat gtttgcctgt gttccattgc ttcttctaaa
     2521 cctattggac ccaaatgaat acttaagaca attgggagaa aatcagaaag tccttctagc
     2581 tcttggtctc tacttccgca aatagcgatt attggttttc gatatttttt tgcaagcact
     2641 gctacaccgt atggaacttt tccgtggatc gtttgctgat cgatccgccc ttctcctgta
     2701 atgatgacat ctgcttgttc gatctttttt tccaatccag tgatttggct gactaaatcg
     2761 aaaccagaaa ccatcttcgc tcccaacaaa aaaagggctc caccaatacc gcctgctgct
     2821 ccacttcctg gaatatcatc taaatccacc tcccagaaat cttttatttg ctgagcaatc
     2881 tttcctgctt gtttatctaa gaaagctagc gtcgtatcta ttcctccttt ttgtcttcca
     2941 aattgccaag cagctccttg ttgacctgta tatggattgg taacatctgc agcgataatc
     3001 aattttactt tagaaacaag aacgtttctt cgatcaaaat gaagtttttt agtagataat
     3061 aaaggatttc cttctgtgta cccttctaaa gaagcaccca aggattggag taatcctaga
     3121 ccaccatcgc tacacccgct tcctcctaaa gtgacaataa tttgttctac ttcttgaagc
     3181 agtgcatctt taataagttc tcctaaggca taactggaag caagtcggat agtttgatcc
     3241 gttggtttat caagtagatc taaccccaat gcttgtgcag attcgatgac tgcggtgttt
     3301 tttccttgcc aagttgtcaa taaatagctg atttttcgct gacggaataa gagatccatc
     3361 ccatcaatgg tacgccatgt tcccctgagc gcgtaataga ttgcttctgc agtattttct
     3421 ccgccatctg caatcggcac attgattttt cttcctttac gatcttccca gccatttaga
     3481 actgcctgat ttagttcaaa tgaagaagca cttcctttaa atgaatcaat tgccgccaaa
     3541 atatccatct gtatccctca ctttcttctt aagtaagaca ctttttttca tttttttcaa
     3601 acaaaacaat gctattttct tccatttttc tatatactaa aagggaactc attgaataaa
     3661 ggagagaaaa aacatgaaaa tcgcaatagc tggggccgga gcaatgggca gccgaatcgg
     3721 attgatgcta catcaaagcg gaaatgaggt cctcttgatc gatcgttggc ctgctcacat
     3781 tgaagccatt cgtacaaatg ggttgattgc tgactttaac ggtaaagaag tggtcgcaaa
     3841 actgccaatc tattcaccag aagaaatcat cgaaagcaac gaacacgtgg acttgatcgt
     3901 agcattaaca aaagccaatc aattagatga tatgttttgt tcgatccaat ccattattac
     3961 tgacaatact tatgttttgt gtttactaaa tggattaggt catgaagatg tactggaaaa
     4021 atatgtgcca aagaaaaata tcttatttgg gatcacgatg tggacagctg gtctagctgg
     4081 tcccggaaaa gtcacattgt taggtgatgg agaaatcgaa ctagagaact tggaaccaga
     4141 aggcgaagca tttacaaaaa aagttgtaga agtctttcaa gaagcaaatt taaatccgat
     4201 ctatagccat aacgttcgtt attccatttg gcgaaaagct tgtgtaaatg gaacattgaa
     4261 tggtttatgc acgattttgg attgtaatat tgctgaacta ggcgcgcaaa aagcggcaga
     4321 gagcatggtt cgaacaatcg tatctgaatt tgcttctatc gcagcaaaag aaggaatcat
     4381 actagatcaa gaagaagtgt accaacatat tgcttctact tatgatcctg acaacatcgg
     4441 gttgcattac ccatctatgt accaagacct gatcaaaaac catcgtttga ctgaaatcga
     4501 ctacatcaat ggtgccattt ggcgaaaagg acaaaaatat gatattgcta caccatattg
     4561 tgcctttttg acccaattag tccatgcaaa agaaggaatc cttggcgcag aataaaaggc
     4621 tctctgatga gaatcagctg aatcagcagc tggttctctt tttttaagcc caataaaaga
     4681 tcaaaaagaa aacacaactc tcatgatgcc ataagaattg tgcttgtttg tttcagtaca
     4741 taatctcttc aaaatgtagg ttttttattt gttttcaaca gaggagaggt catattcgta
     4801 ttgctcataa atagtggaat cgttagtgat aaatcgtttt gcccaatttt ctgcatcata
     4861 aaattccaat agctctgata ggattggctt aaatggtgta tgcagaaatt ccggtaattc
     4921 atctaaatca cgttctcgaa gcaaaaaaat attttcagga cgataaaaat gataatgagc
     4981 aatcgcttga ttatccgtca ctagtttctt ttgaaaatac atggattcaa atgtccgcaa
     5041 agtcactcct tgctgaccct cccgcaacag ttccagaatc ccgcgcgatt cccctgtttt
     5101 tttcaggtac tcttcataag taagatatcc aggttgttca ttccctcgat gtggaagaat
     5161 gaaatagttg gtcgtaacat ctctttttat aaattcctgc ttatagcttt ctgctcgttc
     5221 ttttctccca ttattcgttg caccaaaaaa tagatccgtc tttggttcgc tagaagaaaa
     5281 attcatatgt ttcgaataaa atgaactatt atgtttcagc ccaagttctt ttgcttccaa
     5341 aaaatcaaaa tgataaatct catcgattgc cggttcatct tgtgcctgct tcagcatcgc
     5401 ctcgtgttct gctttgaagt gattccaaaa aaataacaca atttttgcat tggtaaatgg
     5461 gcgcacgcgt cgagccatct ccatatcaaa tccataatct aaaaaaacga tcagctgata
     5521 atttgccaac tgttgtctag ctgatttaat gaattccgac tgtagtaaat cttttccgga
     5581 ccaaacatct aaagagcgga tggaaggcaa aaaataaagt gatttatttt ttgcaactag
     5641 cagtacgtca tttttgttca tcttttccgc ctcactttct ctgtcacttt ttcctatgat
     5701 agcctgttta aatagtaaaa agccagtaaa acgtttccta tatttgtatg aaaatttaac
     5761 atttgtatga aaatatgatg aaataaatca ttcttctatt aaaattcgcc taaaagtttt
     5821 ctgaatagtt gtaattgtct gaaaatttgt tatcattagt cattgttaaa tattgctatt
     5881 tattcttaat ataaattttg attgtgaatt taaaagaaag aggtcattta tggaagataa
     5941 aaaattgaaa aaacaactta gctccagaca tatcaccatg ttagctttag gtggagcaat
     6001 cggtgcaggt ctatttaaag gaagtggcga agcgattggg atcgccggcc cttctgtatt
     6061 gattgctttt ttgattggtg ggctgatttt atatatcgtg atgaaaggtc tgggaaaaat
     6121 cgttttaagc ggtggcgaca cgcatcacgg attatctggt ttgatacgcc cttatctagg
     6181 tgctcattct gctgatttta ctgattgggt ctactggtct atgtggatta tcaatatcat
     6241 cgctgaagct gttgcagctg cttccttttt gcagttgtgg ttcccgcaca ttccagcctg
     6301 ggtatttgtt tttatgttgg ctgttttaac tacgatcatc aatctttatt cggtacggtt
     6361 attcgcagaa acagaatatt ggctggcttt tgctaaaatc agtgtcatta tcttactgat
     6421 catttttgct acttatcttg tcggtcagca aatgttgggc acaggcgttt tccctaccct
     6481 tcagcaattg actgaccatg gtgggtttac tcctcatggt atgaagggaa tcatcagttc
     6541 tttgcttgta gttatctatt cttacggcgg ttctgaactg attgctatca cggtaagcga
     6601 agcagatgat cctaaacgtg cgattcctaa agcaatcaaa ggtgtcatgg gaagaatcat
     6661 ttccttttac atcattcctt tgtttttgct tttgattctt tttccttgga atactttagc
     6721 aggcacgaat gtcagtcctt ttgtgatggt atttgaaaaa atgaatatcc catttgcagc
     6781 ggatatcgtc aattttgtga ttgtccttgc tttgttctca tctatcaatt cgggtgtgta
     6841 tgcatcttca agaattttgt tcttccgttt gaaggatcga aaaggttcct ctagaaaact
     6901 agctgtttta aacaagcatc aagtaccgca acgtgctgtt ttattttgta ccagtgttct
     6961 ttatcttggt gtagctcttt cttattttgt cggtgacaaa ttatttggct atctggcagg
     7021 ttccctttcg tatacagtat tattgatctg gatcattatc agtgcggcat cctttgtact
     7081 ggcactaaga aaaggaacat tatgggataa aggaatcagc ttttttgcat tagctgtctt
     7141 aggattgatt ttccttggta tcctgttcac gaactccatt ggggtaactg tattgactgg
     7201 cctgctttac ctgttcattt atttcagtta tcaaaaaaag aatgatgcgt tcgtattgac
     7261 gaatgaatga aatcgataga aaattttatt ttatttatgt ggaatagatg gagctattga
     7321 agaaaggagt tgtgacaaaa gtcttgttgc aactcctttt tccgaataaa cagtctcatg
     7381 acctcttggt aaacggtgtt caaaaaccct ctgtgcaaaa taattgattg ttctccatgc
     7441 tcatgacatg atagaagaaa ggaatgaaaa aaaggagttt gttttaaatg aaaaagtatt
     7501 ggaagttttt gttgatgatc ggtgtttcaa cggtagttat gtttggtatg atgtacttga
     7561 atgtctatgc gctcgatcat atcttcttca gtcgtacacg agtattcatg gcgttgatga
     7621 tgggtgcagt catggcagtc atcatgctgt tgttcatgtg gaaaatgtat gaaaacaaaa
     7681 agataaatgt tatgatctta gccgctagtc ttcttgtttt tgccggttcc ttgtttatgg
     7741 tacgaagcca aactactgtc aacgacacag cttggatgaa ggccatgatt cctcatcatt
     7801 ctatcgctat cctggcaagt aaacgagctg atttaaaaga tcctgaagta aaaaaattag
     7861 cggaagaaat cattcaggca caagaagaag agatcgacaa aatgaaaaaa atgatcaaag
     7921 aaatggaatg accattatcc gtccaaataa aaaaagagga tgttccagaa gttatcagct
     7981 tcaagaaata agaagaagct acttctggaa cgccgtgaat aaggggttgt gaaaaagctg
     8041 ccctgcatca agtaataaga acagccaaac aaaaatagct tctcctattt ttcgtttggt
     8101 taggcttatt accgcagatg caagcttttg aacaccgttt attaagaggt tgtaaaatca
     8161 gcgttctgac ctgagtatta gagagacaaa tggaaaaata gttcctcata ttttgccatt
     8221 ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tatcgagagg ttgtgaaaaa
     8281 gtcgcttagt ttcgcgtatt aagaaaaatt ttgtgaaatg gctttccaca tttttgtata
     8341 aatttggctt aataccgaga aactggcttt tgaataccgt ttattcgtaa aagggattgt
     8401 gacaaaagtc ttgtcacaat ccctttttat gtttttattg ttctaattgc gctgtcttgc
     8461 ttactgcttc ttgcggtttc acaaattttt ttgagattcc ccaacctgtg aaggcactga
     8521 ccatcgaaaa cacaggagaa agcaaactga aaaatacgaa tggcaagtaa gtgagtgtag
     8581 gaataccaaa tgtacttgct acgaaacttc cggcaattcc ccaaggcact aagtagttga
     8641 ttactgttcc accatcttct agcgttctac ctaatacagt tggatcaaat cctgcttttt
     8701 cataagtttc tttaaatgca ttaccaggca gaataacaga taggaattgt tcaccaataa
     8761 aaatatttac tccgatactt gtcatcaatg ttgtaaaaat caaatttggt gtgttcgtca
     8821 tctttcttga tactcgttcc atcacggttg agatcaagcc aaattcgatc aacaatccac
     8881 ctaatgacaa agttagaata atcaatgaca cagtaggcat catactttcg attcctccgc
     8941 ggctaagcag tgaatcgatt tgttggtttc cagtatttga aacaaaccct gattcgatga
     9001 ccccagccaa cttcgttaag gaagtttgtg ggttttgaat aaaaatcatt cctactgcta
     9061 cagcgatatt caacaaaatc gttaaaattg ccggcacctt tttccatgca cacagtaaca
     9121 ttaaaaataa aggaatcagt gaccagattg aaatagtaaa gtttgcttct agaacatcag
     9181 tcacttcacg aatagatgaa agacttgcgg ctttacttgt gtgtcctaaa atcatgaata
     9241 aaaagaaaga aacaacaaat gccgggacag ttgaccacat catatgcttg atgtgtttga
     9301 acaaatcggc atcaacaata gctgctgcta gatttgttga ttcggataat ggagacatct
     9361 tatctccaaa aacggcacct gaaacaatag ctccaacaac cagcgctgga tttaagccta
     9421 gtgttgtccc aataccgaaa aatgcaatcc caatcgttga cataacagta aaggcactcc
     9481 ctacagcgct acctacgatt gcacacacaa caaatacaga agggacaaac cactggacac
     9541 tgatcaattt gaacccaatg accattagag ttggaatgat ccctgcttgt atccatacag
     9601 caatcaatga accgactaaa ataaaaataa agattgggat gattcctggt ttgatgccat
     9661 tgatgattcc ttcgtgcagc caatctgtcg ggtatctttt tatcactgca tacaacatga
     9721 ccattccgat cgctgctaag atcgtgatat taggtgagac ctttaatccg acaacgccaa
     9781 tcgcaatgcc taaaacaata accattaata caagtgttgc ttctaaaaaa cttggtgttc
     9841 ctttttttcc catgataatc tctccttttt tagttgttct tatgttctaa aaaagttaag
     9901 atatcggatt actccgccgc aattcatccg cgattcctcc tttataaaaa taaaaagacc
     9961 tgttttaaac taaggacgaa tgactccgcg gtaccacctt aattaaaaac agatctgttt
    10021 ttcactttca aacttttatt caaatgtatg gactttccat gtaattcgtc aattaggctt
    10081 gatcggttca cagcgaccac cgactttctg aacaaaacac ctaaccgcta ctgcgtgaaa
    10141 agttgacttg tacgaagttt accttccata agatcatttg tcaatgaaaa agaaaatatt
    10201 ttttcatttt tctgaaaatt atttttataa ctttcacaca caagtttatt ttttactaga
    10261 aagtagcgcc ctcttgtttt tttcttgcta ctatttcttg atgttcttgc ttgattcttt
    10321 ctaatagctt tggctctttc aacagatcaa gagctgtatt ggcgagtagt tctgctgcaa
    10381 tcgcaataga agcaaggcct ttgtcacttt tagcagcagc tttgaattct tctgtgtgtc
    10441 cggcaatata tgtctcagag attgctaccg taggctggat tgttggtatg acttggctga
    10501 catttcctac atcagaagaa ccgagacttg ttctttgttc cgtttctatt tcttcatctg
    10561 gaacacctgc ttcttttgca tgctggaaga ataagtcatc aaaagaagga gtaacgatga
    10621 catcatcaac tgcattttgg aacaatccaa actcaaaagt cgtgcccgta cttatcgcag
    10681 cacctcgtac gatattttcc actttttcat atactttatc taatgtttgc cgatttcctg
    10741 cacgtaaata gaaacgtcca gcagcgtaat ctggaatgac gttggcagct actcctccgt
    10801 ctgtaatgat cccatggata ttgacatctt ttggcaattg caagcgtaga gcgttgattc
    10861 cattaaacac ttggatcaat gcttccaaag catttactcc tttttcaggt gctgctgctg
    10921 catgggaagc gacaccgtaa aatttgatgt cgactggatc attcgctaaa gattctgtcg
    10981 ttttcccata acgaaaagct ggatggacgc ataacgctac atcgacatcc tcaaaaaaac
    11041 cttctcgaac aaagctgcct ttagctgatc cgttctcgcc cccttcttcc cctggggtgc
    11101 catatacacg gatttctcca ccagtttcat caatgaattg cttcactatg ctggcggcta
    11161 atacggagta agtaccaaac aaattatgcc cgcatgcatg accaatacct ggtaaagcat
    11221 cgaattctgc caaaaaggca attgtgggac cgggtttatt ggaaatataa cgtgcatcaa
    11281 aggctgttcg gtgtccagct acttcttttt ctacccaaaa cctttctttt tctaattgtt
    11341 taatcaacgc tgctgaggaa taaaactcat agttacttac ttcaggatgt tcgtggatat
    11401 ctagagcaag tgcttgatat tctggtaaac gatattggat tagatccttc aattggtttg
    11461 ttgagattgt tgttgtcata aaaaattcct cctatgctag tcatttttct tattttctcc
    11521 cgaatgtttc ccatgcaggc acgcttgatc cttttgatgt tttctcgatt actttgactg
    11581 tgtcttctgt ttgataagcg tctactattt tttgatacgt tttattgttt tcatcttctt
    11641 ttcttgcaac gatgatattc acataaggac gtgcatggtc atccactggt tctaagaaga
    11701 tggcatcaga agcaggatta aatccagcat ctacagctac accgctattg atcactgaaa
    11761 tgtctacatc tgacaaagca cgtgctgttt gggaagcatc taattcagaa atgtctagat
    11821 tttttctatt ctcggtaata tcagaaacag tgggcgcttg tccttttgcc ttatccactt
    11881 tgatcaatcc agcagtttgc aataacagaa gagctctgcc gccatttgta acatcattag
    11941 ggattgctac ttttgctccg tctttgatat cagctacatc cttcaaagtg ttagaataaa
    12001 ttcccagcgg tgcattgaca gtatttccga tcgatactaa atcagttccg tgttctttat
    12061 tgtaattgtc taaaaagaat tgatgttgga aagaattcaa atcaatcgat ccatctgcta
    12121 aagccacatt tggttgattg taatctgtaa atttcacgta ttccaaatta atgttctcct
    12181 ttttcaaacg gtctttcacg ttgtcccata catctgtatc ttctccaata atccctagtt
    12241 ttacgatagt ttcttttttg tcagttgtat ctgcttttcc ttggctacac cctgccaacc
    12301 aaataattgc tacaaatgta atgactacaa aacgaataat ttttttcatt ttttctcctc
    12361 cactttcaat gtgtaatttt tttaatcagt tgattactga taaattgact taaaaaaact
    12421 aagattagaa tcaataatgt agccacaatc gttacatctg tttggaatcg attataccct
    12481 ctcgatatag caagattccc taatccgcct gcacctactg ctccagccat ggtagtcagt
    12541 ccgatcacgt tgatgatagt caacgccgag acccgaatga taccaggcaa accttctaac
    12601 aaatacacac gtaaaatgat accgattgga ctggttccca tcgcttctgc cgcttcgatc
    12661 accccaggat caacttctag cagtgcgttc tcgatttgac gagcaaaaaa cggaacaact
    12721 ccaaaaatca atggcactaa agctgctttc gcaccaatcg tcgttccaac taagaaacga
    12781 gtagtcaatg ctaaaagaga aagcaagata atgaaaggga tggaacgaat gatattgatc
    12841 agcttatcaa gtatttcaaa tagcagacgg ttagctagta atccattagg gcgtgtcact
    12901 actaacgtca cacctaagat gattccaatc atcccgccaa tgaatgctgt ccaaaaagac
    12961 atatatagtg tttgcttcgt cgcttcgaca aattcatccg gaatcaaata aacattgggc
    13021 aaatatgttt taaagaaatc agtcatttta tcttcctcct tcgatcactt tgatacgact
    13081 atctaaatcg tttttttgta aaatatttat ttttacacca gatttgatca aataatccaa
    13141 tgcttttttc cgctgtttgg tttctccagc taaagtaacg atcaaactcc ctaacggcgt
    13201 ttcttgtaat aactctacat tgccatataa aatattcgct gttacttgat atttgctgta
    13261 cagatgagcg attagaggtt cgttcgtctg atttccaata taacttaatt ccaccagcca
    13321 tttattggcg atctgatcgg caaaagcaga atgggcaatg atcgtttcta atgcttggtc
    13381 aagatgagta gctgttcgaa taaaatcttt ggtcaattct tcttcaggat tgctgaagat
    13441 ctggatgctg ctaccttttt caacgattct tccatcttcc ataaccgcta ccttgttgca
    13501 gatttctttg acaacttgca tctcatgagt gatcaggacg attgttaaac ctaactggcg
    13561 attcaatttt tttaacaatg caaggatctg aagggtcgtt ttcggatcaa gtgcactggt
    13621 tgcttcatca cataataata ccttaggttc attcgccaaa gctcgtgcga ttgctactcg
    13681 ttgtttttgt ccccccgaca gttgtttagg ataagcagat gctttttctt ccaaaccgac
    13741 aagttccagt agttcattca ctttttgacg acgttcctgt ttcgattttc cagaatactt
    13801 taaagaaaaa tcaacgttgt caaaaatagt tcggctttcc attagattga aatgctgaaa
    13861 gatcataccg atggcctttc tttcttttcg caattctttg ggagagagct gactcagatt
    13921 cgtctgattg acaatgactt ctcctgctgt tggtttctgc agcaaattca tcacacgaac
    13981 taacgtactt ttccctgcac ctgagtaacc gacgattcca taaacatcgc ctttttcaat
    14041 cgttaaatct acagaatcta ctgcctgtat ctctttttct ttttgtttga aacatacaga
    14101 aacatctcgc aattcaatca ttaaaattcc tccttatcta aaaaaagcgt cctcaagaaa
    14161 atgaaaatgt cattttcttg aggacgcttt tttgcgtgtt accacctcat gttcaagctg
    14221 tgttcacaca caactcctct tcaagtacga tcatactcta gcaagatatc ggtcgcagcc
    14281 gtcatcgcct tacatctatg ctcgggatga attgctcgat ggccattttc caatagtcgt
    14341 tccttgcttc ttctcagcta ccgaagctct ctgtcaaagg ttcttctatc gtacttacca
    14401 tttcaatgca aataatattc aaaaaaaaag tccttttact aacataaact atgtcagtaa
    14461 aaggacgacg tatcgcggta ccaccttttt tcggaagttt ccttccctca agcccaacat
    14521 ctcaaaaaga gagattggcg ttcgttttaa cggtcgaaca gttccgaatt tgcctactta
    14581 tcgacaaatt attctccaag gccattttca gcttttctta cttgactctt ctcaccagcc
    14641 agagcctctc tgcaaagttt ccaaacttac tttccttttc aacgaaatcg atattttgtt
    14701 gttgggatta atctacatga ggaaaactga aaagtcaagt acgaaattca aattattttt
    14761 tctatattta tttttctgtt tttttattta acatttcaat aacagttcga agatcttttc
    14821 cttccgcaag aagtttcaat ttttcctttt cttcgacggt ataatttcgg acaaaactgg
    14881 aatcaggtat caaaacaacg cctaatcctg ctcgtgaagc agctgttgct cctactaaag
    14941 aatcttccac tgcaaaggct gaactagctt ctaatcctgt tcgttccaaa gctgtcagat
    15001 aaggaatcgg tgaaggcttg ttctccgaca cttcgtctcc gaatgtaaac gtgtccaagt
    15061 aatcagctaa tttaaagtga tccaagatat ctgttgctct tttcgtaaca gtggtcgtag
    15121 caatgcctag tttcatatga cgttctttta agtctgccaa tatttctttt gcatatggtt
    15181 ttaagtggat ggctccgctt gtcagctgtt gataaaaata ttcttcacgc ttcgctcgga
    15241 cttcctgtac tttttcaggg ctacctgctg ctttcactaa aatatcaaaa ctttgtttcc
    15301 agttttttcc gccccaagcg tcaacgattt cttttgggat atggatatca aattgctgaa
    15361 atccccaaag ccagccatca cgatacgttt tttctgtgtc aactaacaaa ccatctaaat
    15421 caaatattgc acctttcatc tatacattaa tcctttccaa aacaatacac atagtttagc
    15481 acgactggtc caaaaagaac agaagcaaag aaccagcctt ctcgtttata agagttcttg
    15541 gaatcgacgg atatagattt tcttcacagc tgtcacaagc gccagataag cgatgactgt
    15601 agcaaataac cataaccagt aattgccagg aagtggcatc aagcctaatt cagcaccaaa
    15661 ccttgtaaaa ggaaggacag aaccgacaat aattcctaat gtagtgataa caaacatggc
    15721 aaatgttgcg ttgctttgga taaatggtac tttcggtgta cgcagtgcat ggagcactaa
    15781 ggtttgtgtc catagtgatt cgacaaacca tcctgcatgg aataatgcga tgaaaatcac
    15841 tcgctgtgaa ctatccaatg tatggaaact tccgcccact acagcaggac aaataacaaa
    15901 atacatcaac aagtaagtag taatatcaaa tatcgaactt gttggaccaa agtaaaccat
    15961 gaattcccca atactagatg cattccattt ttttggctcg tccaaatatt ctttatccat
    16021 ccgatcccat ggcaaggaaa tgcaggaaac atcatagatc agattcaaaa acaaaagttg
    16081 taaaggcagc attggtaaaa atggcaggaa cgtacttgca atcagcacgg aaaacatatt
    16141 gccaaaatta gaacttgctg tagccttgat atatttcatg atattcccaa aagtttctcg
    16201 tccagaaaga aggcctcttt ccaaaatcgt caaatctttt tctaaaagga tcacgtctgc
    16261 agattctttc gcaatatcca cagctgtatc tacagaaata ccgatatcag ccactttcat
    16321 tgcaggagcg tcattgatac catctcctaa aaatccgaca gtatgaccat ttttacgtaa
    16381 agctgtcgtc aaacgtgctt tttgctgagg attcaatttt acgaatactt catggtcttc
    16441 agctacttgt gctaattcat gatcactcat ttccgagatt ttctcaccgg taaccaattc
    16501 attgatctct agacctactt gacggcacac tgaacgtgta actaattcgt tgtccccagt
    16561 cagaactttt acagctacac catgttcttt cagagctttc aacgcttgtt tcgttgtttc
    16621 ttttggtgga tcaaggaaag caagataacc aatcaaaacc atgtctgact catctttgac
    16681 tgaaaattct ccaacgacgc ttggattggt cttttgtgcg acaccaatca ctcgtaagcc
    16741 atcttcatta agtgactgta cttttgcaag tacggcttct tttcgttctt ttgttaatgg
    16801 tgtaacagta ccgccaagat caataaattt agaaatgttc agcatttctt caatcgcgcc
    16861 ttttgtgatc atttgtgttt taccagctgg atcagcaacc acaacactca tacgacgacg
    16921 ttcaaaatca aaaggaattt catccacttt ttgataattg atcttatctg tatctaaagt
    16981 ttctgttgct tcatcaatga tagccacatc taacaaattt ttcagccctg tttgatagta
    17041 gctatttaga tacgcatggc gcaaaacacg atcatcttca tttccttcac aatccatatg
    17101 atattcaaga atgattttat cttgtgtcaa tgtccccgtt ttatccgtac ataacacatc
    17161 gattgcacca aagttctgga tggcattcaa atttttgatc actgttcctt ttttggccat
    17221 cacacttgct cctttgacca aattcgtcgt aacgatcatt ggaagcattt caggtgttag
    17281 accaactgct acagatagtc caaaaagaaa agcttctagc caatcccctt ttgtaagccc
    17341 attgatgacg ataactgctg gagccatcaa tgacataaat ttgatcagta gcatggatgt
    17401 tttatggatg cctaattcaa agcttgattg aattggtttt tcagatagcg tttctgctac
    17461 atgaccaaat agtgtttcat ttccaacaga aacaatgatt ccttccgcac ttccactgat
    17521 gacattactt cccataaaga caagattttc gtaacttgtt tcgctttgct gatcgtcttc
    17581 acatactttc gcatgctttt ctactggata gctttcccct gtcaatgctg cttgggaaat
    17641 gaataaatcc tttgcggaca ataaacgcat atccgctggt atcatatccc ctgctgaaag
    17701 ccgaacaaga tctccacaga cgatttcttc cataggaatc tctgtgtact ttccattacg
    17761 ttttacagct gccgttactt tcacaagtga tttcagtttt tcagccgctt gattggaacg
    17821 tacggactga atcaacgtca ttgtcccgct gacgataacc atagtgaaga taatggctac
    17881 accgaaaaga tctttatctc caggagctgc taatacatat tccgtgatga atgaaataat
    17941 tcctaatgcg atcaacacta atgtaaatgg cgtgatgtat gctttcaata tttctacaat
    18001 gaatggtgtt tttttgccat atgaaatcgc attttcaccg taattttctc ttgaacgttc
    18061 gatttgttct tctgtcaatc catttgctga tgcatggaat agttcaaata attcttgttt
    18121 cgtttttcca gcaaaagctg catagatttt ttgtgtatct ttcttttttt ccatctcaat
    18181 ctctcctttg tgtccaaaag acagctgttc tcgctgcttt tggtctttag ctattcatcg
    18241 gatcgataga gaaaaaactg aaaggggctg agaacagata gataatagcc ctggcctttc
    18301 ctctagatcc ttgtttttcg aatgactgtc atccatttgt tctcatctcc tttactaagt
    18361 aattttaatt catgacttcc aatcattttt cgaatagtta tttttccaat tagaccacct
    18421 cctttttatt tttcaccgat ggggatttgc ggcaacaaaa aaacctctcg ccaaatactc
    18481 agcgaaaggt ttgaaaaagc atattaaaaa aagcttctca gatctcatcg ttgagttttg
    18541 acactgtaca acgtaaaaag cagatacctg cttttagcta ccttatgaaa agccttatct
    18601 cgacaattcc tgttctaccc attggcgtct ctcgacgttt ttgggcagta gcctatgttt
    18661 gtataggagc ctcacctaac aaatcttatt cggttttcct tcgttaatat aacaactaaa
    18721 aaagaggatg tcaaatcaat tttccatgtt tttcatactt tttttaaaat acacacgtta
    18781 tattcctgtc tcacgcggtt ttatttgtct cattttccac ctcccgctat aatagaaata
    18841 tagtaggaaa cgaggtgctg ttatggaaag aacacttgtt attatcaagc cagacggcgt
    18901 gagacgccat ttagtcggaa gcatcatcca acgttttgaa gcaaagggac tggccattgc
    18961 tgaaatgaag tttgaaacta tgactcctga attagcaaaa gaacattatc agcacttgac
    19021 tgagcgcagt ttttttgatg aactgatcga ttacatgact tccggtcctg tcgtttatct
    19081 tgtgctggtc ggcgaggaag tgatcgatat cgtccgtaag atggttggag caacaaaagc
    19141 tgccgatgct gttccaggta cgatccgagg agactacgcg ctgccgggga ctgaaaacat
    19201 cattcacgct tctgattccc gtgatgctgc ggtaaaagag atcgcccgct ttttcccgcc
    19261 attcgatata aacaaaccgt ctgaaaaagc ggcgaagtga tacaaattct aaactgctgg
    19321 tctagttgct gggttcagca atggccagca gttttttctc taaggacctc cgttgattct
    19381 ttcctaacat atattcgagc actgtgaaga attcttggta gtctttcgtc ttttcttccc
    19441 ctaaagcagc agaaatcaat ctttgataat aatctgccat caatgtttca tagatcactg
    19501 tacttctttc tttatgtgct aagctccgtt tcatatcttc attcacacta cgtgcctttt
    19561 tcaaccgacc attttctata taaaacgaag tcaggttcat taaaaaagta aagagatact
    19621 gctcatgatt gttcaactgt gctaatcgtc gcattctttt tttgattttc aaataattgt
    19681 tctcaatgaa ccgatcactg aatatccaag gaaaatcagc tagaatagat agttcaaatt
    19741 gaccatagtc tggtatctgc atcaagtatt cttcaatgat tcggcattca taacgtaaaa
    19801 attcaaattc tgtctcagat acttctgttt tttcgaccat gtgaagattc aaaaccaaat
    19861 aatacagatc ttctgttttc ttaaaatgcc tacggttatt gctgatctct ttataaggat
    19921 caaagctgtc ttcttgtacc cgatctagca ttcgtctttt tttctttaac tttattggtt
    19981 catccagctg gtttcgcatc cattcatatt ctgcaagtgt catgttcatt cgctctaaaa
    20041 atgatctcaa tgttacaaat gacatttttt tcactgaatt tgtctcaatc ccgactagag
    20101 aagtcttaaa acagattcct tccgctaatt gggactgtgt caacttcttg ttgactcgaa
    20161 gtttcttgat caattcacca tcatacataa tagttctcct ttttaattta aatttttcca
    20221 cattcaataa atagatacgt taaaaaggaa aaatacacga aaaaaataaa aaaatgtttt
    20281 tttcataaaa aataccaacc ttcaagcatt agaatgttgt ctaactactg aaggttggtt
    20341 tgattgttta ttgattattt ttttgctgtt caacgtctgt tcttgttact ggatataagt
    20401 tggttgttgt tttattacca ttttttgaaa agacacttgt cgaatgttcg cctttttgct
    20461 tttcgattgc ttcattacgt tccgcttcat ttttatagtc atattttttc ggatcaatcg
    20521 tctcaaatcc gtttggcaca taaaaccgca agagattttc ctgattcagc ttatctgaaa
    20581 gacttaacgc agtcttgact ttttcctgat ctttggagat ttcctcatcg attcctgctg
    20641 ctttcgtatc gatgatttct cctgtctgat tattgtaagc ttttcctcct aggacggtgt
    20701 atttgggtgt cacaaagttc ccatttcgaa aagcaactac ttgatcatgc tgagaagata
    20761 ataagtctgt accaaaatga agataatctt tatcatcgat tcccaataga tgtagtaatg
    20821 tcggcaaaac atcgatttct ccaccgtatt catgattgac tgtcccttta gtataaccag
    20881 gcatatggat catcaatggc actcgttgca tctgtgcgtt gtcaaattca tcccatgtat
    20941 ctggatcttt accaagagcg cttgctaaat ctttattatc tgtattcgag atcccaaaat
    21001 gatccccgta aatcacaaag attgaatttt ggtaaatacc agattttttc aaatacgtaa
    21061 aaaattgttc caatgattga tccagataat gtgccgttcg aacataatta tctactgtgc
    21121 tattgccagt attcaaagat ggaaagtcga aattcttgtc atccgtataa taaggcgtgt
    21181 ggttggttac ggaaagaaat ttcgtgtaaa acggctgttg tagatgctca agatattggg
    21241 cagattctct gaataaatct ttatctaata tcccataacc taacgtttcg tcggattcat
    21301 caaaataaga tcgatcaaag aaattgtcat aacctaagtt tttatataca tgatctctat
    21361 tccaaaaact agctacattc ccatggaaaa cagcgcttgt atagccttgc ttttgtccaa
    21421 ggatcgctgg tgcggcttga aagacattgt ctgacccaag ttctgtgaat aatgagcctt
    21481 gaggtaaacc aaatgtgcca gtttccaaca tattttcagc atcacttgtc tttccctgtc
    21541 ctacttcatg aaagaaattg tcaaagctga tcgttgcttg attctggaaa atactattta
    21601 aaaacggtgt gacttcttgt ccgtctacct tcatattaat caaaaactgc tggaaacttt
    21661 ctaaatggag aacgatcaca tttttccctt tagcgatccc aaatgtttcg ggatttggct
    21721 ctgcataatg tttctttgta taatctaaaa cttcatcaat cccattactg cttgcatgcg
    21781 cgcgaacact gctagtcatt cctgttttga ttccgtcata aatagtatac gcatcgattc
    21841 ctagatattt gacaatatat gaacggtcaa atgttcgctg cagcaattgt ggacggttgg
    21901 cttcgctcag cgctagattt cctgaaaaaa taagccctga aagacaggta acagcgatag
    21961 ccatcggttt gccgacaggg ttgcttttga taggtatttt cttgaccttc aaccaaacca
    22021 gtagcccaat gaacacagcg atatccagcc agtagatgac atcatggggc ttcatcaaag
    22081 aaaaggagct tcctgacaat ccttgcgaca ctttcgaaaa gcctaataca gatttgactg
    22141 taatgaaatc tgtgaattcc cgataaaaaa taatattcaa gtacaacaaa agcgtattta
    22201 aaatatcaat gatcaaaaga atagctaacg ctggctttgg ctttttaata tacaaagaaa
    22261 ggccaaaaaa caataaggta gtcgcaattg ggttgatcca caagataaag tactggatcg
    22321 taccttccac acctaatgaa aaatctgcat agtaggcaat gatcgtcttc agccagaaaa
    22381 agaaaacagc tgtcaaaacg attgctaaac gattttggca caatttttta agttgtgtca
    22441 cgtatatcac ttctctctat tcacattttt tcttattttg ctcctgaaaa atgaaaattt
    22501 cctaaaaaag ttgttatctt aagatttttt tattctttct aaagattatt ccgtccactt
    22561 ttcacagcat aaaaccgctg cgatgtaagc gattaaacta atgaagagat aaaagtcttg
    22621 tggtgaccgc ttcactccac ggtattcaaa agccagtttc tcggtattcg tacaaaaaaa
    22681 gaggctgtga caaaattctt gtcgcagctc cttatttctt gatgcaggac agctttttca
    22741 caacttcttc cttaaacggt gttctcctgc caactcctcg gccacaagtc acaatattta
    22801 taaaacatga gaagctgttt tttgaaattg ttccttgtgg ctcagagtta aacggcaggc
    22861 tcaagacctc ttgatacacg gtgttcaatc agctgaccct cggtattaga ttatgtcagg
    22921 acgctaattt tacaacctct tggtaaacgg tgttcaaaag cttgcatctg cggtaataag
    22981 cccaaccaaa cgaaaaatat aaggagctat tttttgaaat tgctccttgt ggctcagagt
    23041 taaacggcag gctcaagacc tcttgataca cggtgttcaa tcagctgacc ttcggtatta
    23101 gctcaaaaaa cggcaaaata tgaggaacta ttttgccgtt tgtttctcta atactcaggt
    23161 caaaacgctg atttcacaac ctctgttttt cattgtccat aggattgata ttgtccttgt
    23221 gatgactcat tcgtaataat tgaggcttga ttttcttcac ttcccgttat cgtttgtcca
    23281 ctgccagaat ctgaaggact tgttcctgtc gactcgtttt tttgagaaga gacagttgtt
    23341 ttcttgactt gttcatttgt ctttttccat tccgctaaac gatcggcgac cacttttttg
    23401 tctacttttt cagaataagc ggtgatcact tttccatttt tcaaacgggc tactttgtcg
    23461 ccatcatgag tcaaaagcaa aacggtcaaa tgcttgtctt taaattgata ttgtctgaga
    23521 gaaatcgcac taagtacgtc atcgatatta ataaattgat tttcttcttt tccttttggc
    23581 aaataaatgt ccaattcgcc tttttcgtaa ttggcagtta ttttatactc cgcccaccaa
    23641 taagttgaga attctttttt gaaattatct actgattctt tgcttgctac gattttttct
    23701 gtttctttct gagcaagatc tgctgtcatt tttcctgaaa atgctgcgga taaaataagc
    23761 agtaagaaaa aaccgaataa ccaaaagaga agaaacggtc tttttttcct catgctttcc
    23821 tccactccta tttaattttg actattctat tatatacaaa aaatgaaata gaaagacaaa
    23881 aaagttgaaa aagatcgtaa ataattgaac ttctttttca caaaatggta agaatggaga
    23941 atttcacgca tttgctctca actattcttt cttaagcctc aaatccgttt ttatctgata
    24001 cgtctttgaa ccaatgaccc gattttttga ttgttctttt tccatcttgt gctaagtcta
    24061 ctgaaataaa gccatatcga ttcttatatg cattggtcca cgaccagtta tccatacatg
    24121 tccacatatg atagcctaaa caattggcac cttcttgaat ggcttgatgg acatatttca
    24181 gatgagattg gacaaattcg atccgataat catcgtggat catgccatct tctttgataa
    24241 atcgtgcttc gttttctaca cccataccgt tttcagaaat aaagcagtcg atatttccat
    24301 aatgatctcg tacattgatc aaaatatcat agattccttg ttcatagatc tcccatccgc
    24361 gataaggatt cattttacga ccaggcattt cataattgtc gaaataatcc tcaggcattg
    24421 gaccgttttt agtatcgatt ggtgtttctt ttgcttttac ccggcgtggc tggtaataat
    24481 tgatacctaa aagatctact gtattttctt tgataaccgt caaatcctct gcatggatca
    24541 caggcatgag atcaagttct tttactatct caatcaattc acttggaaac tcgcctttta
    24601 cagctggatc aaggaatgaa cgattgaaga acgcatctgc tatttgcgct gctttaacat
    24661 cttcagggtt tgattcgtct cttggatagc ttggtgtcag attcaagatg ataccaataa
    24721 caccaccttg ctccatttca tggtaagatt tgatggctaa cgctgaagcc aacgcttcat
    24781 gatagccaac ttggatcgct tttttcatat ctgactcttc tggataatgc catcgatata
    24841 aataacctgc ttcgactgga acgattggtt cattatgagt aaaccatttt tttactttcg
    24901 aaccaaacaa ttcaaaacaa cttttggcat atttttcata ggcttcaacc gtttctcggt
    24961 tcaaccatcc gcctttttct tgcagataca tcggcatatc gaaatgatac agattcacaa
    25021 agggttcgat accgttttca atcaactcat caaaataacg atgatagaaa cgaacggctt
    25081 cttcgttgat tgttccagat tcatcaggta tcaaacggct ccattggata gatgttcgaa
    25141 atgaattatg cccagtctgt ttcatcaact gtacatcctc ttgatacttc gtataaactt
    25201 gcgaggtttt ttcaggtcct acttgatcaa agaacttttg cggttctttt ttgtaccaat
    25261 aatcaaaaat gttctctcct tttccatcgc ctgaaatcct tccttctgtt tgtgggccac
    25321 ttgctgcaga accccaccaa aaatttgctg gaaattgata ttttttcatc aaagcacctc
    25381 tttttctttt ttagttcgtt ttagttgatt ccatgtatga tattttccat cacaatcact
    25441 ttttcatttt cttcctcttc ttttttgatt cgttttaaca gctgttctgc tgctcgttcc
    25501 ccgattttat ctgttggtgg cgtgatcgtg attttcggaa ggatactcag atcgttttta
    25561 tctaaataat catagtgact gaacgcaata tccttcccga ttctgacacc ttcttgattg
    25621 aaacattcga tggcaccaat cgacatattg taattgctga caaaaatagc agttgcccct
    25681 ttttctaata atgcttttgc cccgtaatag ccgctttttc tagaatagtc tcctgtaaag
    25741 acctctaatt ggatcgatgg atcattttgg accgcttctt tggctcctgc taaacgttct
    25801 cttgctacgt agtctgtttg agtggcagca ataaatccga tttttttatg tccttgttca
    25861 ccatgatgag cgatcacttt tgctacactt tctcgatcat tcactaatat tgtatcaata
    25921 cctgctggtc cttctggaca attcaatgcg ataactggaa tactcatttt tgtgagcatt
    25981 tcaaatccag accactcttc ttcggataag aagacaatca gcccatcgat cacatgcttc
    26041 atcaggtatt caattttttg ttcaattttt tctggctgat cggcaaaccc gcttaataat
    26101 aagctatacc cattttcttc agctactctt tcgatccctg agacaacttc cgacccaaaa
    26161 cggctggaaa tcccgtttat caaaagtcca atagagaagc ttcgattatt cttcaaccct
    26221 ttagcaatga tgttttcttt atagtccaac gctgaaatcg cttctgctat ttttttcatg
    26281 ttcttttcct tcagttgctg accattcaag taacgggaaa ctgttcccac tgaaacacca
    26341 gccatcttag ccacatcacg gatcgatgcc atacattctc actcctgcca gttacatttg
    26401 atttctcttt cttgttgttc gattatgatg accgcattct ttgcatagtg ttgcttgtca
    26461 tcaatatcca tataactaaa gtcttcagta gagcaacttc ctctcactat taattatact
    26521 taaacaagta tttgaaccgg ttcattttta atatagcatt tttttaaaag attgcaacac
    26581 ttttttaaaa cgctttctga aagtggcgga ttttttgcct ttttaaaatt ttattctaaa
    26641 atttactcta tttgtgggtg ttttgtgcgt ttttctcttc catccgtttg aagttttaca
    26701 ttccaatggt ataatttctt tatcctatca aatgggggaa gtattttgaa gaaattaatc
    26761 gtactattta ttttgtctgc gggacttttg acattgactg gctgtgattt gtttggcggc
    26821 tctgaccaat cctcggacga aacaaccgat tcttctgtct taaaagcgga aaaagcagat
    26881 accaccaaca gtgtatatac gctaaaagta cgtaaagata tggatttatc cgcgggacag
    26941 ttgacattta cagataaaaa aatggaatgg acacgcacgt atcagacaca aggaactgat
    27001 tcttctgaat catctgaaga aaaaacagtg ctgacagata tcaagatcac taccaaagat
    27061 gacgaatata ttatttcagg aaaagaaaat aaaaaagaaa caacagtcat atttacaaaa
    27121 atcggtaatt accgtttgaa ggatgaggat ggcaacatct acagtctata gtgccgtctg
    27181 ttggatcttc ttttgtttct ccccttcttt tctgctatag taaagaagca agatgattgt
    27241 aaaggagacg aaatggatga tcgatagtcc tgattattgt acatgtaata acaacaaaaa
    27301 tatccataca gaatttaatg accaaggaag cttgagcata tgcgacaact gtcacaaact
    27361 agtcaaagat tcatttaaaa catttgaatc ttatcctgtt caaaattatg taaaaagtag
    27421 aagaagataa acaaatccaa gcaaaactca ctatggctga tttttatgaa cttcaaactc
    27481 agcttcttcc tttgatcaag cttcatcagt aaaacaaaca gtggatcatt tcagtttaca
    27541 aatcaaagag taaagattta tttatttcaa atatttgaat ataaaataac caaacgatag
    27601 aaaggcttat tctacatcac gtagtaattt ttctttcccg tcgtttggtt ttttattttt
    27661 agcctaattc ttgacctttt gcatgctggt tatccgtaaa atattttcca gcaggctttc
    27721 cttccaattc agctaatttt tcttggatct gacggatcac cttttttgca tcttctggat
    27781 tttctacaaa atcgaattta tccccatcca cttgtatttt aggacataca tcaaaatctt
    27841 cataccattg attatagcgg gtgttcaacg tttcataata ggtatataac gtctcatcgc
    27901 tttctagctg ttcatagtca cgcccacgtt ttttgatacg ttctagcatc gtgtccaaag
    27961 aaactttgat atggaccatc aaatcaggcc gtttttttgg agcaacatca tctaactcgt
    28021 ttagcatcgt atctaataag ctatcgtact gctgtacttc gatatccgtt acccgaccta
    28081 aatcagcgtt tagatggaac aacaggctat cttcatagat ggatcggtct agcacattgt
    28141 catcgttcac taaagcattt ttcattgcta aaaatcgctt gttcaaaaag aagatttgca
    28201 ataaaaaagc gtactgctca ggatttgaat aaaagagcgg caatacttcg ttatcttcga
    28261 cattttcata aaatggttta gaatcaatct cttttgcaag catatccgtc aaactgctct
    28321 tacctgcacc aatcgttcct gaaatcaaaa tcactgacat acaacccttc ctcttctata
    28381 agtactcgcc tacgtaagtt tcaacaggca atgtttcaaa atcatattca cgtacagctt
    28441 ggtctgattt taaatacgta tggagacttt gtgtaatcat caagttcgta ggtaccgtct
    28501 cttcttgtac aatgatcatc ggttttttca acatagttgc cacacctaat tcgtaagccg
    28561 ttcctggatc gattgtttga tgctcaaagt caaggatagc aacaacaaat tctgcatttg
    28621 tcagttcttc catatctttt tcgtatactt cctgcgccca tccagcgctg aatagttcat
    28681 aattgctttc ttggtgatga cgtggagagt agaaacttgt gactgttttg ttttcctcca
    28741 acgctttttc gatacgactt actcgatcga tttgctcctc tgaaaaaaat ggtcctgcta
    28801 aatatatttg actcattatt ttgcctcctt caataataac tgatttttaa tagatactgc
    28861 tcgtaattca ttgaccagtt ccaatggatt gttggaaaag attacttgat ccaaccattt
    28921 tttatccata aaatcctctt ttgcaaaagt ttctaataat tgttgcaagc cgctgtaata
    28981 accgccaata ttcaatattc cgattggctt gtcgggcgtc agaccgattg ctatccaaga
    29041 aagaagttgt gagaattcct ccaatgtacc tagacctcca gggaaaacga ggacagcatc
    29101 tcccttttca aataataata cctttctttc atcgatcgtt tctgtaggaa taaatgtagt
    29161 aacatctttt ccaggcaatt cagtttcaaa aagcccattc ggatagatac ctgtgacttt
    29221 tccgttattt tccagtactg ttttactaat cactcccata atcccggagt atgaaccacc
    29281 gtaaaccagt tcgatttctt cttgtgccat gtattttcct aacgtttgcg caataaattt
    29341 gtaactttct ttattaccaa aacgggaacc gcaaaaaaca gtaagtttca taaaaaaaca
    29401 ctccctaact caaaagtcta tcttcattct acacgtgttt ttttaaaata aaagactaga
    29461 ttttgtagtt ttttagtggt atataggata gacgccacta tatatagtat ttagaaaaaa
    29521 atcatttttc agccatttta tagataaaac aaaaataaat aacgtgtttt catcgatatg
    29581 aaatgactta cttatagaag atactgggat ttcgtcctat atgccaataa cgtgtattat
    29641 tttaaacaac aaatacttgt ttcttttatt aacgaaaaaa tcattttgtt ctcgtgttat
    29701 gtttcactga taaatgatgt ttctctaaat aaaaaagccg acgaactaaa cgatcaaaat
    29761 ttgatcattt ttgttcatca gcttctccta aaaatctttt tgttaacgat aaatagctgg
    29821 atttgtaaaa atatcacttg aatcgtcact gctggaagaa aattcagtga taaccgcacc
    29881 tgttgcgcct gcctggaact agtgtttgat attgggctcg aacgtgtact gatctctagc
    29941 atgaagaata atttcttttt ttactgtata ataactttct ttcctttttg gaatagtaca
    30001 ggaaagtttt tttgtttcct ttccttcgac ataaggtata ccagaccctt tcgacaacga
    30061 aaggttctca tttttccttc atcgtcattt caaggagggt gccgatgctc tggacagttt
    30121 cgatcgctcc taaaccaaaa tctgtatatt cgattctttc taactctgat tctttcatac
    30181 cattcctgtc tcaaggtaca tctattgtac ttttttcttg taatccattc tgcctctcct
    30241 aactaattgt caaatcatcc caagatatgt cttcttcctc tttttcttct tggatgattt
    30301 ctgcattcgg atctactggt tttttcaata ataaaatggt tattcctgtg acaaaagaac
    30361 cagctaacaa tgcaccaatg aatgcccatg gtctggtcat cgtagcgact gcaaacattc
    30421 ccgatgccgg aatacggcta tctgcgcccc aaaacatgct taatccgcca cctactgtcc
    30481 caccgactcc cgttgctatg acattgcgtg ccaagtcgtt caatacgata gggaaacatc
    30541 cctctgtgat ctgacagatc cccataggaa aagcggtctt caatgtatcg atttcttgac
    30601 gattgagtat atttttacga atgattttcc ctaagaaata agcgatcgtc aagccgaatg
    30661 gggttaccat cgaagctaaa atcaatacag tgattggttc atagattcct tctgaaaatc
    30721 gtgccacacc cccttatcca gaataaatgg taatccagaa acagccgtgt atctgcggta
    30781 ataagcctaa ccaaacgaaa aatatgagaa gctatttttg tttggctgtt cttattactt
    30841 gatgcagggc agctttttca caacctcttt ttttatttat gtttcataat caatatggta
    30901 aggatgcagc tccgtatggt agaccgcctc cgaatattca atcatttccc caatttgcgt
    30961 agacgatttc cgtatccgtt tgatgccgat ctgtgcgtct gttttcagaa ttccttgttc
    31021 ttcttctgtt aaaaatactg ccgagaaatc atcagaaaat gagtcaatag tatatccttt
    31081 tttcgtcagc aaacgataca aagattcttt gccaaattct tctctgctga attgcatcat
    31141 ttcttgtgga agataatgaa caaaataaat ataaggctga ttgttcaaat aatataagcg
    31201 cttaaaacaa acagcttgct caccgaaaaa ggaaaacaaa ggatcttcct tctttatatg
    31261 gaccaattgc aaatccagcg tttctttccg gacttcatat cgggagttct ccaagatttg
    31321 cgtaaagctt gccgctttgg ataatcgatt gtaaggacga ttgcttagaa ccgtcgtccc
    31381 acgaccactt ttctttgcga catattcatc tgatgccagc agttcgattg cctttcggac
    31441 agtgattttg ctgacttgaa acagttcttc taattcactt tctgtcggca acatcgaacc
    31501 gactggatat tttcctgaga aaatatcttc tttaatttta tctgcgactt ccaagtatag
    31561 tacacgtgtc cttttcataa cgtcaccctt tgctttacta ttactaaaag aatagcacat
    31621 aaaaaataaa aaaagaacac aaatcacatg tgagaagtgt gttctttttc attcagctgc
    31681 tttaattaca agtttacgcc atttgaggca attacttctt tataccaatc aaaagatttt
    31741 ttctttgatc gattcagtgt cccatttcct tcatcgtcta gatccacata gataaagccg
    31801 taacgtttac tcatttctcc tgttgatgca ctgacaagat cgatacatcc ccaaggcgta
    31861 tagcccatca aatctacacc gtctgctact gcattagcca tcgcttcgat atgacgacgt
    31921 aaataatcga tccgataatc atcaataatc gtgccatcct cttccatatg atcaatggct
    31981 ccaagaccgt tttcaacaat gaataatggt ttctggtaac gattgtataa ttcattcaat
    32041 gcgattctca gcccttctgg atcgatctgc cagccccatt cacttgcctc aagaaacgga
    32101 ttttttactc ctcccagcaa gttcccttct gaagttgctg cttgtggatc agtctcattg
    32161 attgccgtag acatataata actaaatccg atgtagtcca ctggatattc ttttagcaac
    32221 tggaagtctt tttccgtttg ctctaatttt tccggatcga ctccatattt tttatgggta
    32281 cgcgctgtgt atgcagggta ttctccacgt acttggacat ccgtgcagaa aaaattgaat
    32341 tcttggtttt ggatcattgt tgctgcctga ttgactggat tggcatcgat gctgtaagtt
    32401 gttgcataga taatcatgca gccgatttgg atatccgaac gcaattcgtg gccgattttt
    32461 acggctttgc tacttgcaac gaattgatta tgccatgctt ggaaaatatt ttggtactct
    32521 cctgctccat tgctttttac taatccttga cttaaagctg gaaagtgaaa ggccgaattg
    32581 atttcattga aagtcatcca atatttgact ttactgctgt atctctctaa tactgtctga
    32641 gcaaatcgtt catagaagtc gatcagtttt cgatttttcc agccaccgta ttcttttgcc
    32701 aaatgaagag gcatttcgta atgagaaatc gtgatcacag gttcaatatc atattttaag
    32761 cactcatcaa tcagctgatc ataaaattct aatcccgctt cgttaggagt accttcgtct
    32821 ccatttggaa aaattcgtgt ccaggcaatc gaaaaacgat aacatttgaa tcccatttcc
    32881 gcaaacaagg caagatcttc tttgaaacga tcgtaatgat cgattcctcg atgattcggg
    32941 taaatatatt tttcttgatc aatcgtccag tcaaattctt cactgccgat aatcgcaaag
    33001 cgctgcttac ctccaggcat agcatctgca acggatagtc cttttccgtc tcgatcataa
    33061 gccccttcta attgatttgg cagcagttgc tccaccccat aaaaattttt ctgaaaaacg
    33121 tgattgcatc ctgatttcct ccttatttta caattgccag aacatcttcg tttgcaataa
    33181 tctctttttg gtctaattca agtatatcgg caaatgcatc agtattcgta atgacaactg
    33241 gagtgattac gctatagcca gctgctctaa ttgcttcgat gtcaaaacga atcaataaat
    33301 ctcctgcttt gacgagatcc ccttgtttga ctaagatctc aaagccattc ccatttaatt
    33361 ctactgtatc catcccgata tgaatcaaaa tctccgctcc gtcagtcgtt gtgatcccta
    33421 cagcatgtcc agttggaaaa atagtcgtga tctctccgtc tgcaggtgca actagttctc
    33481 ctacttctgg atcaatagcg ataccttttc ccatcacgcc agaagaaaaa acttggtctg
    33541 gtacttcact caatggcagt actttacccg ttaaaggact ttttaaggta tgccgtgctg
    33601 aagtaatcgg ttctcctgca ttcgcatgtt tgttttctaa ttgattgtct tgtgtttgtt
    33661 cgtcaaatcc taaaatcaat gtacctacga atgcaagaac aaaagcaatt cctgtagcta
    33721 ttactgctac tgtcacattt gactctacac catccaccgt attaatgaaa gtcggaatcg
    33781 tcaataagct gaccaaactg ctggagaaag cttttactcc ggaaaatccg ataatagctc
    33841 caccaatacc tccagaaata caagcggcaa tgaatggttt cttcaaaggt aatgtcacac
    33901 catacacagt tggttcagtg attccaaata acgaagtcac tgtagaagaa atccctaatg
    33961 ctcgaagttt cgtatctttt gttcgcaatg ctacagctaa agcagcccca ccttgtgcca
    34021 agatggctgg aagaagcatt ggcatcaata catcaaaccc atattgttcg atattcaaaa
    34081 acatgatcgg tacgaatccc caatgcatgc cgaacatcac gaatacttgc cataaacttc
    34141 ccatgattag acctgctacg attggactaa atccataaat cgagttgaag agaccgccta
    34201 aagcattacc ggcaaccgtc ccgattggtc ctaaagcaag aaatgttaat ggaaccatca
    34261 ctactaaaac gacaagcgga actaaaatca tttgcagaaa ttgcggaatc acttttttta
    34321 cgaatggctc taatttactt tgaacccata cggctagaat gattggtatg acagaagaag
    34381 tataaccaga tggtgaaaga atcactggga tcccaaagaa actgatagct tctcccgcac
    34441 cagcaactgc tgtgatagct ggatacacaa gtgccatagc aattgctact gctaaaaact
    34501 ggttggcttt gaacttttta gctgctgtaa aagcaagcat gacaggtaag aaggtaaaga
    34561 caccatctgc tgcagcaaac aatatttgat aagtaccgct atcagctgtc agccagccca
    34621 tcaccgttgc caatgataag aatcctttca ataccccggc ggctgccatc gcccctagaa
    34681 aaggtgtaaa tatactagag ataatatcga tcagacgatt caaaatattt ttggactctt
    34741 cttttccatc ctccgaaact gcaagacctt cctcatccac gatcgattga taaacatctg
    34801 ctacattact gccaatcact acttgatact gaccgccact ttgtacgact tggatcacgt
    34861 ccgggtcttc ttttaacttt tgcgtatctg ctaaagattc atcttttaat ctgaatcgca
    34921 accgcgtagc acaatgtact aaacttttga cattcttttg tcctcctacc gcttcccata
    34981 cacgacgacc aactgcttga ttatccattg ttttttcctt ctttcttttt tatttaaaaa
    35041 ataaaaaccc agattgaaaa aaacagtcac aaacattcgt gaacctattt tttcaatcca
    35101 ggttttgcct gccatacagt aacaatcctt atgattggat gctttttttc tcacttacta
    35161 ctctttgaat atgaatcgtt aaataaatct gctctgcgat ccccatttcc tgtttttgcg
    35221 tttgtcgaag atactcattg atccgttcga cacaatgaaa cgcttttgga tactttcctt
    35281 gaactaatgc gtagagaaat tcatcttgct cgtcagatcc ttcccctttg agataacgct
    35341 gggcaaaaaa ctgcagatga gtaatgatac gctgataatt caaagattct tcatcgaaca
    35401 cgatgccgaa gaatttactg atgatactca gtatatctct taccattgtg gtagcggtca
    35461 tcgtcacttg catgtcttcg tttccttgtt ggctattcac aaaatgaaag gcaataaaac
    35521 cggcttcatc ctcagaaagt tccacacccg tcttttgttt cacgaacaaa agagcctctt
    35581 tcgctgcttg atattctttc ggataaaatt tttttgtttc aaataataat ggattaggca
    35641 gttggatatt ttcttttgct cgtgtgatcg cataatgaag atgatcggtc aatgtcagat
    35701 aaatattagg cgaaaattca acttttagtt tatcttcagc cagttcgact acttgtttga
    35761 cccattcgat ctcctctgcc ggaatatcag ccatcagctg ctggaattgg tttttagcaa
    35821 aagaatctct taaaacaaat tctttttgga ccgcttctgg cgaaatctcg tccccaatct
    35881 ttttttgaaa agctagtcct tttcccatac aaacgacttc ttccccgaat ccatttttcg
    35941 aaatgacgac attgttattc aaaattttat caattttcat tttgcttcgc cctctcaaga
    36001 aaaataaaaa aacctagaat tcgcctactg tttcttagct gaattctggt tttgcctgcc
    36061 gtacagtaac aatccatttc ttattcaaaa tcatcatacc atcgaatttt tatttatgca
    36121 agccttttca tataaaaaat aaaaaaactg gaacacaagt ctatctagaa aacaaccaaa
    36181 tcagagatgc acctctttgc gtttctcttt ttttcagcaa attggttttt ccttatcgat
    36241 tcatatcctt tggctgtttt taatctgtaa cgacttatgt cccagtatcc taaaatgttt
    36301 ttgtctctag attgaaagca gatctcatat catctgtcgc atcttttatc gatgtaacac
    36361 tttccagttc tccttgtaca actagtcgcg tgacggcgtc catgtcgcca caagtaatta
    36421 gtgtgacaat ttcttttccc tcgacttcat tcagtaattg ggtatctgta ggagcaactt
    36481 tttctttaaa aaatgttttg tatgtataca cattttctaa atctgtcaaa tagatcttgg
    36541 cacccatctc taaattttct agcggtgtaa acagcaattc agggttcgtt gcacgatggc
    36601 ttgctaaggc ataatttcct tttcccattt cttgatctgg agataatgtt cctgctccgt
    36661 aaagtagtga cgtattatcc aagcctttga aaaccggtaa attgattgaa actgaaggga
    36721 ctgcgatact agcaatcact ggtagatctg ttcctctcat ttgcgacctc atgacccctt
    36781 ctgtcgtcat tggttctacc gcatcaaagt caaaactgac atctttatcg ttattctttt
    36841 tcagatcttc ttttgttaca ttcgctatag catatttatc tcctgtctct ctgacaaaat
    36901 aatccttgat ctgttcattg aaaatcaaag ctagtcctac taataacaag aggaacaaga
    36961 ggatattgac tagccatctt tttgcttttc cttttttttt cattttctct ccctccagac
    37021 tcattttact gtttttcttt tgctctttcc aacagccgaa tgacacattt ctacaactct
    37081 gtgtattttt ttcgtacacc tgcgcttttt tctacaggat atttcaaatt atttttgatc
    37141 aatttttctt caatgatctc atctagatcc aggtgaaggt tgtctgccat catgtatgaa
    37201 tagatcaata catctgccag ttcttctttg attcgttcta attgtgtttc ttttacttct
    37261 tccggcgttt tccattgaaa aagttctaac aattcagatg cttccaaaga aatagagata
    37321 gccaaatctt tttcgttatg aaattgtcgc caattacgtt catctctaaa ctggttgact
    37381 ttttccatac tattcatttg cttgccttct tccaatcact tcttacttga ttaatcatac
    37441 cattttttta cataaaacaa agtataaatt tctaattatc tttttatatt ttcttaaatg
    37501 ctcgtaaagc cttattctat gtgctttcga gtatttttac tgtaggaaga tacttcacgt
    37561 ttctttgcat atttcctcat gtcttagctg tcagaagtgg taaataagta gtaaattcat
    37621 ttgtactact aagcaacaag acgctcctgt tgcttctctt tattcaagcg tttcatttct
    37681 gccattgcag aatcgaatgt tgcatgtgcg taatagttca gcgtcatggc tatattagca
    37741 tgtcccataa tgtactgtaa tgcctttgga ttcattcctg catttgcata gttggtacag
    37801 aatgtatgtc gcaaactatg tggagtgatg tgtggcaatt tatcctcgtt atacttattg
    37861 tatttcttaa caagaccttt catcatgccg ttgtaatcac ttgccacttt tggatagttc
    37921 tttctattaa gaaagaggaa atcactatat ccatcaatct caacacgctt atcattcttt
    37981 cgattcgcta acactcgctt aaatgcttga taggcttctt caaccatagg aacttgacgt
    38041 tcgccacttt tggtctttgg tgtttcaatg tagtacccaa tttcagtatc tctcaatagc
    38101 tgatggtcta tattgacaag acgattctca aaatctaaat ctggaagtgt caaaccacca
    38161 aactctgaaa tacgaagacc tgtttttaag agtatcagaa tttcatcata atttttgctg
    38221 taggttttat cagcttttgc aaaggctaac agtttttctt cctgttcttc tgttagtacg
    38281 gtcttaggga cagtatcatc atcaagaact gctttcagtt gaaagtcaaa tggattcttc
    38341 cgaacacaat catcttgtat agcaatatag aatgaagcct ttaaagaacg tttgtagtta
    38401 ttgatggttt gataagcata accattttca ctcattctaa tagcccattc tttagcgtct
    38461 gatggcttaa tactgtcaat gcttcttaca cctaacttgt ctttcttcaa aatatccata
    38521 agatatttgc gtccagtttc agtgtttttt ctaacctttg gtctttgagc gttctgtttt
    38581 gcgtaaagct ggcagagtgt cattttcttt cctacaacat caataccatc atgaatgtct
    38641 ttctgtaact ctgcgatttt ctctctaagt gagatacaat cacgctttcc tgctggtact
    38701 cggtctgtag ccacaagttt ccacgagtaa acaaattgcg gttctccaaa tgaatctata
    38761 tatttgtata agtatcttcc gtcttttcgt tggctctctc cagtctttaa gattcgacct
    38821 ctattgtcac gtcttttttc tgacatggca tttgctcctt tcctttatgg aaagagcctt
    38881 gatacgactt aatactattt tatcatatac aagacccttt ggcgacgcta gattgcgtcc
    38941 aatgtatcta taattttttc aaattgtttt cgtttaatct gaatacgatt gccattcata
    39001 atcagccaat ttgcattttt attttcctct gccaagcgtc gtagcttgtt ttcgccaata
    39061 cgaaaatatt ttgacgcttc ttcaatggtt agggtataac gttcccaaat aggaatgtca
    39121 gtctgcttca taaaatcctc ctttccaaat cacttatttg gatttcataa aagttgtttt
    39181 accagcaatc gaacagcttt agcaaagctc acgggagttc cacccctgca tggttctcat
    39241 gtagccatac tcattgcctg cgacggtttt atcacgctcg gactattgac tgtatgggag
    39301 tatcattatc acgataagaa tgtcgttgca ggcaatcctg ctaaagattg cttctcggat
    39361 cactaacatg aatcgctcgc tatctttata agataggtca tggcggttag ttccgttggc
    39421 tcttttctta tcgaaacgta ttcgattact tttattcagt tttcaaagaa caatggctcg
    39481 ttagcctatc aaaacacatt gaaagctcaa tatgctttgg tggaataaca aacctccctg
    39541 ttcgggaagc gtggaatggt ttagcacgct tccacgaaag gagagaggat attacttaat
    39601 ttcaaatgac aaaatctttg taatcagtct ggtttccatt cttccacgta agacttcatc
    39661 aacgaccata ctttgattgc catattcatc tttcataagt cgtagggaac gcttcgttat
    39721 gtaccctctg taatgatgta gaatctggtt aatcgcttcg gtatcgccat ctgttgcctt
    39781 tacaatgaga ggaaagggaa tcataggata ttgtgttttc attcttcaaa ttcctccata
    39841 aactttttaa ttaaggctag tccactggtt ctatgccgat agacagtaga acggttcaat
    39901 ttcaacaggt ctgcaatttc tgaatcgctc atgtccataa agtaaaacag cagtagaatt
    39961 tcacgtttct tgtctggcaa ctcacgtaat gcttcactca acaaatcatt ttcaacgcct
    40021 actgataacc cattgagtgt aaaaatctga aagtcagttg aatagttatc tgttgtcgca
    40081 aactggctaa caagataatc gccaacatcc gaaaaggaca cctcacgctt tgcaatcctt
    40141 gaaagataaa gcatataatt ctttcgctcg tcttccatag cacgtttaca gatatagtca
    40201 aactgatttt ctattgtggt ctgaaaagaa gatggtttca tgtttctcac cccctttctg
    40261 tctaggaaag gaagtgagcc ttgctcgttt atctcctttc actcttagtc ccaatgtgaa
    40321 agggggattt gttgcattac tgataaataa actttgtaaa aaagttctga atagccaaaa
    40381 aagcatataa acagatttat ttctctgttt acatgcttct gttattctat ctatatgatt
    40441 tataaaacca cattggtgga cgtacttatc tattgcagat agacgacttt ttttgacaag
    40501 aacccaatgt aaggaaattt attgtatatg atgtacttca tggcgacgtt gacctccaac
    40561 aaaccgccat ttggaagtaa tatacaatat tttaacagcg taaatagcac taccatataa
    40621 cggttttttt tattggcgtt tagtagtgct ttttatta
//
LOCUS       DAMYXM010000018        39762 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.018, whole genome shotgun sequence.
ACCESSION   DAMYXM010000018 DAMYXM010000000
VERSION     DAMYXM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.018"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(110..655)
                     /locus_tag="PNS21_001121"
     CDS             complement(110..655)
                     /locus_tag="PNS21_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDI5158841.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(667..1359)
                     /locus_tag="PNS21_001122"
     CDS             complement(667..1359)
                     /locus_tag="PNS21_001122"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDI5158842.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1378..1662)
                     /locus_tag="PNS21_001123"
     CDS             complement(1378..1662)
                     /locus_tag="PNS21_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158843.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1675..2238)
                     /locus_tag="PNS21_001124"
     CDS             complement(1675..2238)
                     /locus_tag="PNS21_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDI5158844.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2466..3128
                     /locus_tag="PNS21_001125"
     CDS             2466..3128
                     /locus_tag="PNS21_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDI5158845.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3252..4448
                     /locus_tag="PNS21_001126"
     CDS             3252..4448
                     /locus_tag="PNS21_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDI5158846.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4549..5925)
                     /locus_tag="PNS21_001127"
     CDS             complement(4549..5925)
                     /locus_tag="PNS21_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI5158847.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5962..6828)
                     /locus_tag="PNS21_001128"
     CDS             complement(5962..6828)
                     /locus_tag="PNS21_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HDI5158848.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6917..7942)
                     /locus_tag="PNS21_001129"
     CDS             complement(6917..7942)
                     /locus_tag="PNS21_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDI5158849.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7960..8634)
                     /locus_tag="PNS21_001130"
     CDS             complement(7960..8634)
                     /locus_tag="PNS21_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI5158850.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8818..9711)
                     /locus_tag="PNS21_001131"
     CDS             complement(8818..9711)
                     /locus_tag="PNS21_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDI5158851.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9785..11482)
                     /locus_tag="PNS21_001132"
     CDS             complement(9785..11482)
                     /locus_tag="PNS21_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDI5158852.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11475..12788)
                     /locus_tag="PNS21_001133"
     CDS             complement(11475..12788)
                     /locus_tag="PNS21_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDI5158853.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPQAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESSEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMDELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12788..17296)
                     /locus_tag="PNS21_001134"
     CDS             complement(12788..17296)
                     /locus_tag="PNS21_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDI5158854.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEGELPLQVVV
                     DERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSGQALQRIQRQLFNGILER
                     LGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDG
                     ENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYN
                     GAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASM
                     SELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSD
                     IFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVV
                     GDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFT
                     HPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAI
                     NTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTP
                     RYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPG
                     ENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQV
                     KLDAGAQDGNFYTVNNMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQ
                     NGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWI
                     REIAGPPRYQLVYETYQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYY
                     EGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFEL
                     YETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPD
                     SFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENW
                     QYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLY
                     RAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNG
                     YRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLEL
                     LKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKE
                     VKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQ
                     QELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVR
                     QSKKEGAR"
     gene            complement(17594..18112)
                     /locus_tag="PNS21_001135"
     CDS             complement(17594..18112)
                     /locus_tag="PNS21_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDI5158855.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18114..18731)
                     /locus_tag="PNS21_001136"
     CDS             complement(18114..18731)
                     /locus_tag="PNS21_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDI5158856.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18728..19582)
                     /locus_tag="PNS21_001137"
     CDS             complement(18728..19582)
                     /locus_tag="PNS21_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDI5158857.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19579..20490)
                     /locus_tag="PNS21_001138"
     CDS             complement(19579..20490)
                     /locus_tag="PNS21_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDI5158858.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20531..21865)
                     /locus_tag="PNS21_001139"
     CDS             complement(20531..21865)
                     /locus_tag="PNS21_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI5158859.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTYTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            22073..22903
                     /locus_tag="PNS21_001140"
     CDS             22073..22903
                     /locus_tag="PNS21_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDI5158860.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23147..23465)
                     /locus_tag="PNS21_001141"
                     /pseudo
     CDS             complement(23147..23465)
                     /locus_tag="PNS21_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23587..23781)
                     /locus_tag="PNS21_001142"
     CDS             complement(23587..23781)
                     /locus_tag="PNS21_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158861.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSGF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24286..24471)
                     /locus_tag="PNS21_001143"
     CDS             complement(24286..24471)
                     /locus_tag="PNS21_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158862.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25321..25662)
                     /locus_tag="PNS21_001144"
     CDS             complement(25321..25662)
                     /locus_tag="PNS21_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDI5158863.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25662..25892)
                     /locus_tag="PNS21_001145"
     CDS             complement(25662..25892)
                     /locus_tag="PNS21_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDI5158864.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            26179..26517
                     /locus_tag="PNS21_001146"
     CDS             26179..26517
                     /locus_tag="PNS21_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158865.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(26722..27297)
                     /locus_tag="PNS21_001147"
     CDS             complement(26722..27297)
                     /locus_tag="PNS21_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDI5158866.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(27906..28487)
                     /locus_tag="PNS21_001148"
     CDS             complement(27906..28487)
                     /locus_tag="PNS21_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI5158867.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(28670..29296)
                     /locus_tag="PNS21_001149"
     CDS             complement(28670..29296)
                     /locus_tag="PNS21_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDI5158868.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LIESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(29318..30649)
                     /locus_tag="PNS21_001150"
     CDS             complement(29318..30649)
                     /locus_tag="PNS21_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDI5158869.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(30804..31205)
                     /locus_tag="PNS21_001151"
     CDS             complement(30804..31205)
                     /locus_tag="PNS21_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDI5158870.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(31291..31497)
                     /locus_tag="PNS21_001152"
     CDS             complement(31291..31497)
                     /locus_tag="PNS21_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158871.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(31592..33094)
                     /locus_tag="PNS21_001153"
     CDS             complement(31592..33094)
                     /locus_tag="PNS21_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDI5158872.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(33112..33567)
                     /gene="argR"
                     /locus_tag="PNS21_001154"
     CDS             complement(33112..33567)
                     /gene="argR"
                     /locus_tag="PNS21_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDI5158873.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(33665..34282)
                     /locus_tag="PNS21_001155"
     CDS             complement(33665..34282)
                     /locus_tag="PNS21_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDI5158874.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            34503..35876
                     /locus_tag="PNS21_001156"
     CDS             34503..35876
                     /locus_tag="PNS21_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5158875.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            35951..37063
                     /locus_tag="PNS21_001157"
     CDS             35951..37063
                     /locus_tag="PNS21_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDI5158876.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFEEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(37135..38772)
                     /locus_tag="PNS21_001158"
     CDS             complement(37135..38772)
                     /locus_tag="PNS21_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDI5158877.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLLDEAKESLENLIQKLN"
     gene            complement(38940..39548)
                     /locus_tag="PNS21_001159"
     CDS             complement(38940..39548)
                     /locus_tag="PNS21_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDI5158878.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT        11428 a         8434 c         6895 g        13005 t
ORIGIN      
        1 taagtttttt taaaataacg tataaaaaag agcataaaac aaagtcgttt gttttatgct
       61 cttcttttat tattttgtgc tttttatttt atcaaatcac tttgtttttt tataaaattt
      121 ctgcgcctag tgtggactca aaatgtctca atgcccattc atgtccaacg ggatcgaaac
      181 ttgccacggc atctttgaaa atatggagtt tgtatcctag attatacgca tcgactgctg
      241 tatgcaacac acagatatct gtacatacgc cggtcaaata aatatctgtg atctggcgtt
      301 ctcgcaaacg gatgtctaga tctgttccac taaaagcgga ataatgccgt ttgtctatcc
      361 aataaacatt ttcctcttgc tcatagtctt ggtataaact ttttaaagag ccgtacaatt
      421 cccgtccacc cgttcctatc acattatgcg gaggaaataa gcggttctcc ggatgaaagg
      481 aatcttttgg atcatgcgca tcaatggcaa aaacgacaaa atccttttga tcaataaact
      541 tctttgtatg agcagtcagc tttttttcaa ttacttgtcc tgctgtccca gtagtcagct
      601 ttccatcctc tgcaacaaaa tcatatgtgt agtcaatcga gatcaatgcg ttcatcttag
      661 tcctccttat acgagatgtt tgacaaattc gatcaccatt tctgctgatc gtttgcctgc
      721 gtcttcgata aattcgtcaa agctttgtgt cgccgagtga tctgctgtgt cgctcattgc
      781 acgtacgatc aaaaatggga tattgaactg acgagctgtt tgtccaacag cggctccttc
      841 catttcgcaa gctaatgctt cagggaaatt cgttaagatt tctttgattt tatctggcga
      901 gtcgacaaat gtatcacctg taacgatcaa tccttctttt gcgttcaaat tcgttgcttt
      961 tgctgcttta accatttcac tgcgcaaata tgtgcttgct tcataatata gaggcattcc
     1021 tggtaattgt cctgggttgt agccaaatcc tgttgcatct gcatcaaaat aagctacttt
     1081 gtctgatata acgatgtcac ctacttgtaa gccttcaccg attccgccag ctgaacctgt
     1141 attgatcacc atgttcacgc cgtattgttg gatcaacaga cttgtggtaa tagatgctaa
     1201 gacttttcct ataccggaac gtacgacgat cacttcgtgg ttacctaatg aacctgaaat
     1261 aaataaagcg cctgcgcgtt cccatgataa tggttcgctt aggttctctc tcaaaatttt
     1321 tacttcttct tccattgcgc cgataatacc gattttcatc tttttaattc cttctttcta
     1381 tataaatgcg attgctaaaa aaaccacaca taacattagg ataacaataa taagagattt
     1441 catcaaaaaa gagttccatt tggtcgtctt ttcccgttcg ctgatccttg ttttcgtgat
     1501 tggtttattc tttttgcttt ctttacgata aaagctggca atcttttttt cttcttgctg
     1561 ataagctgtt tctgcttttc gctgtttttt taatgactcg cttgcttgtg cttgttgtcg
     1621 cttgcgaagt tcgcttcgcg taataagcgg tcctttggcc ataagccgat ccctctactt
     1681 tcctttggtc aatagttccc agtattgaat cgcataaatc gtctttgcat cacaaatcaa
     1741 ctgatctttc atcgcttgtt gcgcctcttc aagagttaga tgatacaact ccagtacttc
     1801 gtcttcatct tgtgctaaag gattctcgac cttctctacc ccttgtgcat gataaatatg
     1861 aaggacttca tttgcaaaac ctggtgaaag atacatcgaa gtaagatggc tcagcgactt
     1921 cgcacgatag ccagtttctt cttctaattc tctagctgct gtcatttcgg gattttgtcc
     1981 ttctcctgga tcgatttttc cagctggaat ctccaaaatc actttttcta aaggttttcg
     2041 aaattgtttg acaagaagca gacgatcttg ttcgtcaaaa gcaatgatcc cgacaccgcc
     2101 aggatgaaat accagttctc tcttcgaagt tcctccatct ggcaaacgaa cttcgtctac
     2161 agcaacatca ataatttttc cgctatagat ttctttgcga ctgattgttt tttcttcaaa
     2221 ttgctcattg ttcagcatat cttctcctgc tttctttgta atcataactg cttttttttc
     2281 atttttcaat ttttctcaaa aactccttat tagtttacca tagataaacc ttagattttc
     2341 tctaaatata gaaaatcctt ctttagtccg atactctttc tagtagtccg tatgctaaaa
     2401 taagtatgta atcgaataac gaaaagaatt caatactcaa cgataaatga catggagggt
     2461 ttattatgat aaaacgaaat tggccgtggc ttctagcatt ggtcttgcta tttctctttg
     2521 tccgaaacaa tagtttttct tttgttctag gacttttatt gataggtgta gttttagttt
     2581 tgtggggact ctttggttcg cgcaggaaaa agggaaaaca agaagaaatt cctgcactga
     2641 ctgacgaact tgaaactcac tatgaaaaaa gcggtatgac agcgaatgaa atttcatttt
     2701 ttcgacaaac aatgaatcaa acaaaaaatg agatcaatca attgcaaatg aatatgcagc
     2761 agacagccaa acttaaagca atcgatctcc gccatgatcc agtcaaagca gcgaaagctt
     2821 tattcaaaga attggtgaaa gaaccaaacc gactgcatga agcaagtcag tttctttata
     2881 cgcatctgcc gaatctagtt gatttgacaa ataagtataa tgagatcaat gaccatgaga
     2941 tcaaaaacaa acagacctat gaaaaactag aagaaagcgc gcagatcatc gaccagctgt
     3001 cagctttaat cgcacaagat tatcaaaaat tcgttgctga ggatctagat gatatcgacg
     3061 tagagatatc tgttgcgaaa cagagcttaa agcgtgataa taaatatgaa ccagaacaaa
     3121 aggattaact cttttgttca taaaagaaga ctcggaacat tcagatgatg tctggctgtt
     3181 tcgagtactt tttctaaagt agagacattc atgtaagaat cgagaacaga ttcaacaagg
     3241 aggaattttt tatggatcat aaaccgactg aaacaaaaga tgttacacca gttaacgata
     3301 cacttgaaga tttattgaac aacccgtttt caactccaac agatacgctg accacttcac
     3361 aacaagcgga gattgatcag ctccaagagc aacagacagc tgctcgttta attgacaaac
     3421 taccggcgga acgccaagaa caagctaaac agctagcagc aaaaatcgat gctaatgatg
     3481 cacagtctgt catcagctac ggatcggctg cccaagcaaa attaagtgaa ttctcacaat
     3541 cgatgttgaa tcatgtgcaa gcacaagata ttggtcctgt aggagattcc ttaactgaac
     3601 tgatgtatcg tctccaagaa gccaatcctg acgaactgcg tgccggagaa gggaacatct
     3661 tccagcgtgt cttcgggaag gtcaaacaat cgatctatga agtgacagca aaatatcaaa
     3721 agattggtgc acaaattgac aagatttcag tgaaattaga taaagaaaaa gacggtctgc
     3781 taaaagataa tctgatgtta gaacagcttt accagaaaaa caaagactac tttgatgcac
     3841 tgaatattta tatcgctgct ggagaattga aaatggaaga acttcaaaca acgatcattc
     3901 cagaagcgat gaaaagagca gaagaaactg gcgatcagat ggatgttcag attgccaatg
     3961 attacacgca attcctagat cgtctggata aacgtacgca tgatcttcgc ttagctagac
     4021 agatcacgat ccagcaagca ccacaaatcc gtttgatcca aaacacaaat caggccttgg
     4081 ctgaaaagat ccaagcatct atcgctacag ccattccgct gtggaaaaac caagtcgtga
     4141 tcgcattgac cttgcttcgt caaaaagatg cagtaactgc tcaacgccaa gtctctgaaa
     4201 caacaaacga cttattgaag aaaaattcag aaatgctgaa aatctcagcc atcgaaacag
     4261 cgaaagaaaa tgaacgtgga atcgtggata tcgagaccct tcaaaccact caaaatgatt
     4321 tgatcgaaac aatccaagag acattgcgta tccaaaaaga aggaaaagaa aaacgtcgtc
     4381 atgcagaagt agagcttgga cacatggaag aagatctgaa acaaaaactg ttggatttaa
     4441 cccaatagat cacctttcgc ttgttactaa aacaaacgcc caacaacagt ttattacaac
     4501 tgttgatggg cgtttttctt atctttcttc taacttctcc attggagtct atttctcatt
     4561 catcttgctg tgtccatagc catagccaaa ataaatgatc agtccaataa ccattacgat
     4621 accgaacacg atccatgtca cagtttgcag acgaagcatc aatacaatac aagtcaagat
     4681 ggagacgatt ggcaatagcg gaacgaatgg cactttgaat tctccagctt ttggtttgcc
     4741 aaacattttt ctcaacttca gtaagcctag actaacaagc atcaaatagg cgagcgccat
     4801 gatattcgtt agttccgcta atacattcaa tgggaaaaat ccggcaaaga atacagctgc
     4861 cacaccagcg gcataagtcg cttttttcgg tgtacgatgc ttttgatcga tttctgtcat
     4921 aaacttagga agtaagccat ccttgctgac agcaaacagt aaacgagcaa gtgaatacat
     4981 catcgagatc gttaccgtca gtagcgttaa aattgcccct acagcgatca ccgcgcccac
     5041 tgttccatgt cctacatacc tcattgcaaa agccactggg tcattgactc ctagttcagt
     5101 atacggaacg attcctgtca agatcagcgt cacgatgatg taaaggacag tagcaatcag
     5161 tatcgaacca atgattcctc ttggcacatc tttttgcggg tttttgactt cctcggctgc
     5221 cattgcaacg gcatcaaatc ccaagaaagc aaaaaacaca agagctgcac catctagcac
     5281 accggaaaaa ccaaaaggca tgaatggctg ccagttatca ggtttaacga aaaatattcc
     5341 tacaatgata aacaacgcga tgataccaaa tttgacatat accatggcat tattcaaacg
     5401 taatgctttc ttggcttcaa gcgacacgat gtaagtgacg aatatcacta cgagcaccgc
     5461 aatcaagtca atgtatgtac cattttccgg attgtacccg ccagttaatg cttgtggaag
     5521 ttcggcccct aaacttttca aaaaaccttg catatagcca gaccatccgg aagcaacaga
     5581 ggaaaccgct agtaaaaatt ccccgatcaa caaccaccct gtcagccagg cagcaaattc
     5641 accaaataca acatacagat aggcatatgg cccaccaatc actggcactc gcgaagcaaa
     5701 ttctgcgaaa cttagtccag acaaaatgac aacgattgca gctaagacaa atgaaagtga
     5761 cagtgctgga ccagcatttt gattggccgc aacccctgtc accacaaata ttcccgtacc
     5821 gatgatcgca ccaattccaa gcatgatcaa atcagacgtt tttagttctt ttttcatctc
     5881 actcgtatga ttgagattga tctctttctt acgaaacaaa tccattgaag ttcctcccca
     5941 ttttaggttg gaaaacagcg attaaactgt tttcatatac cctaaaagga taccacctaa
     6001 aatgactaaa aggcaaccaa caataacata aatgagctct tttttcgttt ttctttcgcc
     6061 aaggatgaaa atcccaccta aagtagagat aatgatcccc atttgtgaaa gtgagaagcc
     6121 aacagctaaa ccaagtgatt tgactgttag aagcatgcag atgtttccta agccccataa
     6181 taatccacag atcatgtttt tccacacaaa ctgatcgact ttaactttac ggaaagcaaa
     6241 gaagcttgcg cctaagatca ttccgatact ttgaggtaag ataattgaca tcgcatccaa
     6301 attcgcccag ttgacgatga tggtatatac accataacca atcgtagaga agatcaatgc
     6361 tctaaaaccc ttaccaaaat caagcatttt attatctgta tctatttttc cttcgtcatc
     6421 ttgtcgggca gtcaaatacg caccgcttac aagaagagct aaagcaataa taccgtatac
     6481 ataatctttc gtttgtgtcc attcatggaa gaaaacggct cccgcaatcg tattcaaaat
     6541 taattgaaaa ccagtagaca atggtagccc tacagaaaca cccatgtact tcattccatg
     6601 gaactgtcca ttttgtccaa cactccaagc taagccggat aaaaatccga tcaagaaaat
     6661 ccaaggggtg attacaggac taacaacgaa aaagacaact aaagaaaaca gaaatgcacc
     6721 gatcgtcatt cctagtgttt gttgattggc atcgccacca attttaccac ttactaaacc
     6781 aatactaccc catgccaaca tagggactaa tgctattaaa atttccataa ccaatctcct
     6841 tttatttcac atgataagaa acttatcagc gaaaacgtta acaacacaag cacttttttt
     6901 cgtttctaac gattttctaa cgtttttcat tataaaaatt taagattggg aaaaataaag
     6961 tcattgttgt cccttttcct tcagttgatt cagccgtcaa atggatacct aatttttcgg
     7021 ccagattttt tgctaaataa agtcctaatc ctgttgaatg tgttttgcta agtcgaccat
     7081 ttctcccagt aaatccctta tcaaagatcc gtttcaagtc ttccgctggg ataccgatcc
     7141 ctgaatcttt aactgaaagg ctgactcctt gcgcttcctt ttctatgctc acaatgatgt
     7201 ttccgtgatc cggcgtatat ttgatggcat tactaagcaa ttggttgaag ataaaagcga
     7261 tccatttagc atcggtcaaa acagtatgat cctcaccaac gatctcgtag cgaatatttt
     7321 tttggataaa ataatttgcc tgactgcgta tcacaggctg aatgattcct tttagtccat
     7381 attcttggat gaggtagtcc cgcgagaaac tgtcgagtct tgcataatac aaaacttgtt
     7441 ctacgtattc atcgattttt gataattcat tttctagtaa tgtatacttt tgatcgtcaa
     7501 tatcgaactc gatcgaacga actagcaatt tagaagcgga aagtggcact ttgatttcat
     7561 ggacccatga gtcaatataa tctttttggt cttgttgatt actgattgct gattgcatta
     7621 cttcttggtg ttcacggatc agctgattga tatattcttc taccagtttt tcttcttctg
     7681 tcctcccacc actcaaataa ttttgcaaag gggaagtttt ttgtatgtcg gctaattttc
     7741 cccaccatct tctcttgctc aagtaaccac ccagcagata aaaaatcaga aagacgccct
     7801 caatcaaagc taaataagca attgtgctca aatcaacttt gatgtttggc gccagccaca
     7861 tcacaaagat cgtcaatgca ataaaaaaca gccatcccaa gatcaatgtc cactgatctt
     7921 tcaaatattt aaaaaaattc atcttaaccg ctcatccttt cagggaacga tatatccctg
     7981 tcctactttt gtttcgatat atccttggat acctgcctgt tcgatttttc ggcgtaaccg
     8041 attgatgttg actgtcaatg tattgtcatc tacaaatcgt tcgtcatccc acagtgctcg
     8101 cagcaatttt tcccgactta agatctttcc attgtgcttc atcaaaataa ataatagacg
     8161 atattcattt ttgctcaaat caatgatctc tccgttgatt tccatacttc cgttgtcaac
     8221 attcaacgta atgccattgt ggctcatcat ttcactagat aattctgtgt agttgtaaga
     8281 acggcgaagt aatgcattga tttttgcaat cagtacatcg atttggaaat gttttgtcac
     8341 aaaatcatct gcccccatgt tcatagccat gatcatatcc atattcgtat tcctgctgga
     8401 gataaaaatg atcggtactt tcgatacttc acgtattttt tgacaccaat aatagccatc
     8461 caaaacggga agattaatat ctagtaatac aagctgtggt tcttctcttt caaagtctga
     8521 gaacacttga ttgaaatcgg tcgttccaaa ggtttcaaat tgccatttct caagttcctc
     8581 catgatcaat tgtcgaatga tctcttcgtc ttcgactacc atgatttttg ccataaaaaa
     8641 tccctccatc tttcttcttg ctactacaat atcaaagaag cccttgtgaa acaagttggc
     8701 ttacctggaa aatgtcattt tagcaaacgt taaaaaaaac tggaaatatg cgatttaatc
     8761 gcgtttgtct cttccaagca gcttaaattt attatgttat tcttaataat tggtctatta
     8821 tctgttattt ttctttcgtt gtttttgtct tttaaatttt cgataacacc aataaccgaa
     8881 aagcgttagc actagcaata tacctagaag gagtataagt aatcgataaa gatggtattt
     8941 ttttgtctgt tctatctttg aagagacttc ttctgtaaag ggaactcgtc ttcctgtcac
     9001 tagtaatcgg tgagtgttca cggtgtaagg tgtacaagtg ataagtgtga ctagatcctg
     9061 cttttctcta attgctaatg tatttgtttt tgttggaagg acaacaattt tctcaacgac
     9121 ttcataagcg agcatccgac catacacttc aagatagaat cggtcatctt gctccatttc
     9181 ttcaagatcc gtaaagagtt ttttctcagc aataccgcta tgggcggtaa tcacggaatg
     9241 tgtactctct cctccagttg gaaaagaggt gccttgcagg actgtcgctc ctctatctaa
     9301 caaaagatca ttcgtttcat caaataaagg caagctaaca ttgattttcg gaatatagat
     9361 ggctccgatc atatgttgtt cgtaatattc ttttcctgga ttttggacat ttctaaccgc
     9421 ttgctcaaaa ggatctttga cttgtcccat tcctgggatt actgatgtat gagcaagcgt
     9481 ctgatttttc ttttcatgca ctgctaattg ttgctcatgt ttttcttctt tatcgcgttc
     9541 aagctgcttt tgataattct cgatccttct ttgatccaaa taattgctga gagcatctgc
     9601 taaaaatgga tatgtaaaaa ctgcagctcc ggcaagaaac atcactgcca taaataattg
     9661 taataaaaga ctgatttggg tttgaggtct attacctatt ttctttttca ttttcttcac
     9721 cttcttattt aaaaaaactg cagcaacctt tataaaaccg attgctgcag cttatttttg
     9781 ttgcttatac ttcagacttc attttagatt tacggtacca gctgtacgct ccaatcatca
     9841 aacttattcc gattagtaag aatgccaaga gcccgttccc accagtagca ggaagtgacc
     9901 cgcgtttcat attcggtact ctttctggcc ctggattttc tactcctacg atctgaattt
     9961 cttcattata gcttccataa gagatgataa attcagtaaa ggctgcctca cctgttggaa
    10021 gtacataacc ttctggcgcc atggtctctt tcatttgata ggttccataa tctaaccctt
    10081 gaacctcaaa gtatccattt ccatctgcga caaattctgt tgcttgtccg cttgttgccc
    10141 actcaattct atctgctcct gattcataaa cagctaaagg cgactcccct ttataaaata
    10201 ttgcgtattc agaacggtct tcattccaca attcgaatct tgcatcttta agcaattcac
    10261 tgcttgatgc atcatatttt tcaaactttc ttccacctgt tgtgactttc tctggcggct
    10321 ctactccttc ttcataatca aattcatcat ttcgtccgat ttcaataacc gctgtgttgt
    10381 tatggaaatc atccgcttct agttctgtgg aaaccttcaa attatacgtg atcgttaaat
    10441 ctcctccacg tgcatctgct aatctaccta aagtatctac tttggctact ccattttctt
    10501 ctgtcaaaat cgtcacgaca aacccgttgc ttactacttc tactatataa tcttgtcctt
    10561 ttgttaaaat cgtctcgccg gctcgcactt ctatttcctg atcataataa gttagaccaa
    10621 ctgtaggcgt atctgtgatt ttgtaatttt tgataacagc gcttccgttt tcaaggacag
    10681 aatcgatgaa ataaggaata ggtgctgtga tcgtgtaaga agccgttttt cctctttcta
    10741 gatcaatata agaaatcgtt tcttgttctc cagcttcatt aataatcgtt acttgtctaa
    10801 ggtcgctttc ctctacattc atctgtttat ctgcttctct gatttcgttt ttcggataca
    10861 aatgcacatc tgtattgtat gtgtctttcc atgtagaacc atcccacacg ccatccacca
    10921 tatcaggcat catcactggc atcataacaa ccattggaga cgcgattctg gaaatatgag
    10981 ctggcgaata agtttctaag aaaagataga ctttatctct agaagaaggt gcttcttccg
    11041 tcacttggac ggtagagaat accgcttctc cagcttcatt cgttgtttgt ttgtcaatac
    11101 ttgtcagtcg atattttaaa aaccaacttt ctgtgtgccg tttttgaatc cattcaatcg
    11161 cttcggcaga attaagaccg tcttctcgtt ccggtgttaa aggattatct cgaatcaaat
    11221 cgtaatatac ttccgttacg tcaaacaaat caaaatcgac tcctggaata ttttcttcac
    11281 caaaatccgt agaaaccaat ccgctattcg gtcgttcttc cggaacagat gagaaacctt
    11341 ttttgtgtaa tgtaatcgtc acttcacttg gtttttgtgc gacttcttcg gctttgacta
    11401 gttttccgtt cccgaaaatc cctattcccg cagtaacgag taacagtaca ctcattcccg
    11461 tgacaatcat ttttttattc atttttttct ccccgttctt tttttattaa taaaacagca
    11521 cctaacaata aaagaattcc tagtaaagaa atcaacgttc ttgcttcatt cgtttgcggg
    11581 aatacggacc attttcctga ttgacctgga ttgcttggac cactcggctc tttcggtttg
    11641 tccgttgttt cttttacggt ctcgttataa acacgtggtg ttgcggattc ataggcagag
    11701 actggaactt tcccggattc taattcatcc atctcctgtc cgttaactac tacatattgt
    11761 ggttggcctt ttgcatccac ccaaaattca gggatgatga cctcgattct cttacttgct
    11821 tcatccatct cgtaaccttc cacacctttt aattcttcaa aataataggt gccagaaggc
    11881 aagaaacgtt gtcctgtcgt aacaaggccc tctttatccg aaatgaattt gcttacattt
    11941 ttgtcattca aaggatcatt gtctgctggt ttgatccatt gatttttcaa gtcattagca
    12001 ggagccatat ctaaataata tttcccccct tctatcatct gatatagagc aaaaacagca
    12061 ccttctaatg gttttccttt ttcagttgtt cctctttgac gtccatactt gaagaagtat
    12121 ggatctctta gataaccgac atttttagga tagatatgaa tctcttgcag ttcctcgctt
    12181 gattctgtcg ggtgataaat tggcaaaata gcagcaattg ggatagctgt ctgatcgata
    12241 tcaacatcca atccaacttc tggatcaatc gattcttcga aaatcaaata aactgcatcc
    12301 cgatcttggt ttttcttagg tgctttgata cgtgcaatcc cgtctcctaa tccgccaacc
    12361 gtatctttcc cagttgttac cgaagtccct atttggacaa gcctttcttc tgtgattagc
    12421 tgacggatat tgtttctgtt cgtttcagcg atttgctgga cgaattcttt aggagtcatg
    12481 ttttcagcag cttttttttc atgataataa tcggtcatgt cgtaaatggc aaatgtcgct
    12541 ccatttagtg gcaccgtctg tgaaatgata tctgctgttt cccctgcttc ctcagagata
    12601 gccaagccat cattttcatg gtattcaaat tgatcgttat aatcgatatc tcgaatcatc
    12661 cgtttatgca acacgaattc cacgacatct tcccctgatt cttctgccga catcgcctgt
    12721 ggaagaagga ctgctgcaag cggtagtaaa acaagtaatt gaagcatcaa acacaacagc
    12781 tttttcatta ccttgctccc tcctttttag attgtctgac aaacaaacca taggctgctc
    12841 ctgcaagcgc catgaagaat gcagcaagca ggatatagaa taatcgtccg gatcctcctg
    12901 tagcaggcaa cggattttca ggatcgttcc cgacactaag ctgtaatctg attcgatttg
    12961 tctccaaaag ttcatattct aactcttgct gtagatcagg cgtctctgct tcttcgtttg
    13021 gataatacga aatctcaact tttagtgtac ccgcttcttt tcctggaact acccgatcat
    13081 tttctatgtc aggttcttct cgataactaa tttctaaaat aaagtaacct ggaagttttc
    13141 tatagctgtc aggtgacttc acttccttga tccgatagac cccttccaac aagtctgaag
    13201 cattgaatag ggaagaatcg tttggatctg gtgccaagcc tccctcgatg atttcatact
    13261 ctcctgcact attttgtttc tcaattgaaa attctgcgcc ttcaagacgt cggctattca
    13321 tactgtcaat tttcaaaagt tccaaatcga ttggcgctag ttcatcttct aaggttactt
    13381 gtagtacacc atcgatgatt tcatagcctt ccggcaaggg agcagtttcg tcttcgatga
    13441 ccgtatcttc aatgataaac ttaccttctt cattcagtgc aaactggaac acttgatcag
    13501 atagccgata cccatttggt gcgacagatt ccgagacctc atattcacca atagacagat
    13561 gcagaggttg gtcgtaattt aaatcgacat acttttctac tcctacggca cgagaataga
    13621 agtatccgat cccaggcaaa cgtttcgatt cttctggttc taacggatta ccagtgtatg
    13681 ctcgatacag cgggaaggaa gaatccccca ctttcctgat atcgaaacct gcaccatcta
    13741 atgtatggcc ttcatggtct aattttctaa ctcggatctc ccgataatca aacgtattgt
    13801 aagcagtgaa tgctacccat aaacgatcct cttcatctgt tgtatacgtg agatattgcc
    13861 aattttcttc acctgtttgc ttgagttgga tagtccctat agcattatcg taaagtcgca
    13921 agatccatgt gtcattttca tcaggttctt tgaaaccttc tgggttctgg ctctcaatga
    13981 ttttgtattc tgcatagttg gtagtttctt cactatcctt ataaacaaat gaatctggct
    14041 ctccgattgg atgaataact ttctcaccat ccacgattac atatttcccg tcttcatcta
    14101 ctgaatagaa cgtgagtctc cctttctctt cctctacttc gttcacacca gaaagccctt
    14161 tcgctactgg atatccgatt tccagtccgt taccatctgt ttcataaagt tcaaactctg
    14221 cgccattgat tggcatatta ctgccttcaa ttagtttatt gatattcatg tggattggct
    14281 tgaattcatt tcgcatttcc agtttgacat acaactcatc atactctgtg ccttctatgt
    14341 taggctcttg tacaacagaa agcttgtgat attctccttc ataataggaa ttcggatctt
    14401 taccttccgg tccgccgact ctcgaaccag atgtatattc atctacaagc tcgtagtgac
    14461 ctaacatctc atcttcgttg gctgcatatt ttttgatttg gaattgatac gtctcatata
    14521 ctaactgata acgtggtggt ccagcgatct ctctgatcca aaatatgtcg ctgactgctc
    14581 cagtttcatc tcctaaaatc ggcatcttcg tttcaaagat cgctattccg tccgtggtag
    14641 ttttacttgt ttcgattggt tcggcttccc cactctctgg attttggtca tataatgcaa
    14701 attcgactcc agccaacggc tcgccatttt gacccttctt gctgacttgg aaattacggc
    14761 ctttatgttc gtaacgaatg gaagaagatg ggaaatacat ccgtccagta gtgatatcat
    14821 tctcataatc ctccaggttc tgaatatagg ccatattgtt cactgtgtaa aaatttccgt
    14881 cttgagctcc cgcatcaagt tttacttggt acttgatcct gaccatttcc ccttcaccta
    14941 aagaaatcgg agaaaattta taagcttggt tttcccgtcc gacatacgtt gtcggagtcg
    15001 ccactacttc tggtggggct tgtccatgac gttcttcatc ccatatttct ggctcttggt
    15061 acttcatgcc gttttctcct ggatctagat ggtaaaactg aatatctgcc gatctgccag
    15121 ctccaaatgg tgagacatta aaaccagttt catcataaag tgtggcttgc tggatcgttc
    15181 tcttgaaggt agaagagata tcctccaagg ctgtaacaat gtcattcttg ttgttcgcat
    15241 tgtaatatcg cggtgttacg ccatcttctt ctaaatcagc aatatttttc aatacatttc
    15301 gtccaatagc atctagtcgt tgtctcccgc taacgtttcc atcgattcct aaaccaatgg
    15361 agaagatact tggcgggttc tcttgatact tgtttttcag ccaatgatga taagcaactg
    15421 tgttgatcgc agatgacttt tcttgcgaag taggactgcc attcgtattg ttattagaag
    15481 aaccatcacc aacccagtga gtcgctttat tatctccttc ttttacctct ccataacgat
    15541 agtgtacatt tacttcatct tctggacggt taaagtcaga tgcataggta actggatgag
    15601 taaaccctgc attagaagta ttcccagaaa actcctcaaa atttctgaac aaaacgtcat
    15661 tatcttcagc aatcttatct tccatcacag tccagttgct ccaagctcca ttagtgctcg
    15721 tacgataact tgactgaatc ggtgcataac tgaaggtcgg tgtcccatct cccacaacga
    15781 ttaggatttt ttctggattt ctattttctc cgccattgtc tttatacaat gtttcatagc
    15841 catttttaag ccctagtgta agcggagttc ctccgattgg tgtacgattt aatgctgtat
    15901 tctcacttcc cgtcaaacga ttgatatcat ttgtcaatgg aaagatgtcc gaactgattt
    15961 gctcatggtt attgatagct gtactattat ggtaaaaatt caccataccg attcgaatat
    16021 ccagactcgg gtcagagagc agatctttac tcatttcgtt tactgcctca atcaacgcag
    16081 catttttagt ttgactattg gtcccagcgg ttaattcact catactcgct gacttatcta
    16141 gaacaaaaac gatatcgatt ggactgattt cctttttctc gcccccgatg acatctagat
    16201 agacattgta aagggttgtt gcatcttcag gatgtagatc agatgctgtg ctgcttggca
    16261 gaacaggttc tacccatttt ttgatgtacg ccccgttata atccaagtaa ctgttttcaa
    16321 aattccgttc atttccccaa agctgaacgg tttgagctgg aacgtttcta tcttcttcgc
    16381 ctgcagttga tccattatta gtagcatagt cgtaatttcg ccagttcgct cccccgtttg
    16441 tataatcact atcatcgatc aagttctctc cgtcatatgg cgagcctttg tctccttgat
    16501 agcgttcatt gctttgtgtc caatttccta acacttttgt atagtcattc gttgtaaatt
    16561 tcgggtagcg atgctgttcg ttttcaactt ctccgctagg atagatatat tcataggggt
    16621 cttgcgtagc tgttggccca agacgctcca aaataccgtt gaataactgg cgttgaatgc
    16681 gctggagtgc ttgaccgctg ggtacgattg gttcgtccag cttttccatc tcattttctt
    16741 cgctacgttg aatttcagcg gaatcttctg tatcagtttc attttcttgt aattcttggt
    16801 tatctattct ttcatcaacc accacttgaa gaggtagttc cccttcgttc acatcgggga
    16861 tcttttctgt ttcaaaaatc aatgtgccgg tttctgcttc ttttgaataa acttttccga
    16921 cataccattc ttgaccatcg atcgtctcga cttttaggtc ttctccatct tcatcatagg
    16981 aggatagacc tgttccacga acgtttttta cggtaccaat cccggcggct gccgcatcga
    17041 ttgctaaacg tattctacct tcattggcac tttccttttt ttcaaaagcc agatgccatt
    17101 caagggtttc ttctttttca atatacttcc cttcaatttt tgcatagtcc tcatctaaga
    17161 atacctcatc ctcctttgtt tctgttgact cagcagctac atccagggtc gtcatatgat
    17221 agactgactg gaaatatggt ccaaatagca aactgacagt tactaactgc aaaagcttca
    17281 attttaaccc tttcacatcc ttatactcct ttcaaaaata tttgtcttct ttcacaacaa
    17341 tcaacaaaac aatgcccatc taattataat ttaatttaat tttaatttta aatcaaatga
    17401 tatccttttt agttaaaaaa caccaattat acaacattta ttattaaaat taaattataa
    17461 aattttataa cgttctcatt taacaaaact taagttatta agacaaatga ataaacaact
    17521 acgctttcct cgaaacaaaa aaataaagca caaatgcgca tttacgcact catgctttat
    17581 tcgtacaatt aattcagata acggcagcgc ataggatgcc aataacactt cctaaaatag
    17641 ctcctcctaa aatgtcagta ggataatgta aaagtaaaaa aatcctcgaa taagcgatca
    17701 aacaagcaaa taaaaaggct aatattcctg caactggact taattcgaac aacgcagcta
    17761 ctgataaaaa ggctagcgct gtatgtcctg aaggaaaaga agaagtcttc ggtttacttg
    17821 tgtaaaggac aagccgacga tttttttcat aaggtctttt ccgtcttgtc gtttttttga
    17881 taaaaagcaa accaacaaaa aaatagatca aactgccgat aagaatggct agaccatatt
    17941 ctctagtatc aggtatcaag actaaaacag cagcaaggag taaccaaata aaaccaccat
    18001 tagagatatt actgataaat ttcaaaaaat tgatttttcg actatccgtt ttccgttgct
    18061 tggcgaactt gttgataata ctatcatcga ttttagcaat caaacgcaac atgttattcc
    18121 tcctttttaa gaaccttttc ttgcgtacga tggataaaaa gcaggacttg aaaataaaac
    18181 aagaaactga aacctaataa agcaaaatag attggtcctg caaaccagaa tgcgacaaga
    18241 aggatcaacg tgcctgccaa atagaaaagc gcgcttttga aatcattaaa aaacaagaaa
    18301 aatgcagtaa tccaagaagc ttctttttgt tccagttgaa gcaagatttt gtaactaaag
    18361 agaagcgcgc ttaatagaag caaaaacaac gtagtataca tcaatgtata gccaattccc
    18421 ccaccccaat agcggaaaat ctgataatcg atcaatagaa gaagaaaata aacacttgct
    18481 aaactatctt gctttccata aagagagaag ttctcacgaa actttttcca gaatagtgaa
    18541 ccgagctttt tctcttcatt ttttttgtaa tgataaatca tatacagaac tgttcgactt
    18601 gcagttccta atgtgaaaac aggcaagacg cacaggaacc atagtagctg taaatggata
    18661 agacgtggaa tccattcaat gatttctaag aaataatcat tggctttttt accaatcgat
    18721 cgattcatca tccttttgaa cctcctgtca aatccatccc ttcaatgaaa taacgttgtg
    18781 caaaaaagaa cagcaaaatc gtcgggatca agacaagtgt tgctcctgcc atcagataat
    18841 tccattgtgt cgttgcttgg ttctgaaaaa cttgcaagcc aatctgtaac gtgtattttt
    18901 cttgatcttg gatatataac agaggaccta agaaatcatt ccaagctgcg ttgaaggaat
    18961 tgatcgcaat ggtaatcaag gcaggtttcg ttaatggaag catcaaatga ctccaaatat
    19021 acaaatgatt cgctccgtcg atttcagcag cttcgatcaa ttcattattg atcgataagt
    19081 aaaattgacg catcagaaat atattgaacg cattgccgaa gaacgatgga acgatcaggg
    19141 gcaaataagt acctacccag ccgatcttgg agaataatac atattgaggg atcatcgtta
    19201 caaaccccgg gatcatcatc gtagaaagaa ccaaagcaaa catcagcttt ttcccaggaa
    19261 aatcaagctt cgcaaatcca taagcaataa aagaatttga aaggacatta ccgatcacta
    19321 ctagcacagt gatgaataat gtatttcttg catagcgcca aaaaggaaag gcggcaatcg
    19381 tatccagata gttttcccat acgatttttt ctgggaaaaa ggtcggagga taacgaacga
    19441 tttcttccat tggtttcaat gaagtaaaca ccatccaaag caatggaatg atcaaaatga
    19501 ttcctattac tgtcatgagg acataactta gtattttttt tgtttgccga ttcacactgg
    19561 tctttttgaa ataatccatc atttgccgtc acctccagaa taatagaccc acttaggtgc
    19621 aagcttcaaa ttgacaaacg tcagaaccaa cacgatcaga aacaggatca ttgacatagc
    19681 catcgcataa cccatatcaa aagctacgaa cgccttgttc cacatatgca gattataaaa
    19741 aagcaaagaa tttgcaggtc cgcctgttcc atttttcgtc atgacataag cttcttgaaa
    19801 aatttggaaa gaaccgatcg tagaggtgat cacatcgtaa aaaataatag gagtgattaa
    19861 tggcaaagta atacttttga actttcccca aacgcctgct ccatcgattt ctgctgattc
    19921 atataaagat ctaggaacat tttgtaatgt agctaagtaa agtagcattg ctcctccaga
    19981 actccatatt ttcatcaaaa tcaacgcagg tttcgtccat tgtggatcaa acagccaagc
    20041 tggtccaggg atatggaacc agcccagaaa tgtgttgatc aatcctgcag atggagaaag
    20101 cagctgcatc cataagatgt agactgctac tcctgataag actgccggta aatagaaagc
    20161 tgtacggaat acactgattc ctttgatttt ttgattgagt actgtggcta aaaacacact
    20221 gaaaatcgtc gtcagcggta cattgaagat gacgaaataa acggtgttgt ataacgattt
    20281 ccaaaataaa tcatcgtgaa cgaacattct tttgaaattt gctagaccaa tgaaattcat
    20341 cttggatgtc aaattataat cggtgaaact accaataact gaaaaaagca taggccctag
    20401 cataaatacc aagaacccga taataaaagg cgagataaat aaataggccc atcttgcttc
    20461 ttttttcttt cggatagata aacgcttcaa attttcttgc ctcctctgta cctagacagt
    20521 caaaaatgga ttattgattt tgcttgacta agttttctac tgctttttgt gcatctgcca
    20581 atccttcttt tggtgatttt gttcctaaaa gaatctggtc aatctgttca ttcactaatg
    20641 tcgtgtaatc cggagcatct aaaggaactg gtgtgatcac cgtattttta aagttttcgt
    20701 ccgccatttg atagatcatt tttcctttat ccgttaggtt gtcattattc actaaattct
    20761 cattggctgt cttgctcgcc atgatatcaa aacttttttc gccgaatttt tcttgtactt
    20821 ctggagaaga aagatatttg atgaattcat aagaggcctc aggatctttt gctccgtatg
    20881 gtacttccaa gacgaatccg ccaccccacg accaatggcc tgaaccttct tctttttctg
    20941 ggagctgcat cacgccaaaa tcaaaatcat ccggtgcatt ttccatcaaa ctagagtaat
    21001 aattgatatt ttgggcacgc atggctacta atccggaaat aaatggatca gatacaccag
    21061 acccaaattc ggcttctaat ttattgatcg tatttttccc ataataatct tgccagtctt
    21121 ggatccactc caaggcttcc actttatttt tagtatcaat cttgactttt tcatctttgt
    21181 caaaccaact agtcccgtta tctgcgttta gcgcccatac atcagcccct aaattccata
    21241 atggataaaa accgatgcgt gtaaattctt ttccttcttt tttatctagt ttacgtgctg
    21301 tttcttctaa ttcatcccaa gttgtcggta gatcttgttc actgattcca gcttctttaa
    21361 ataacttttt attataaaaa atgacttgtg tatatgtatt gaatgggatc gcataaggat
    21421 caccttcata ttctactgta tcccaaagct gcggataaaa atccttagaa gtgtcttttt
    21481 ctacatattt ggagagatta gttgcttgct gtgcttctgc tctttgggca acggaattta
    21541 tgtcctgtac aataacatcg ggtgcatttc cagctgttat tgcagataaa gatttagtcc
    21601 agatgtcacc ccatggctga tacttataat ttacatggat ctcgttttgt gattgattga
    21661 aatcctcaat gatttcttcg atagcttctt ttcgtgcacc ggatccccaa aaggaccaga
    21721 aatcaatatt ttttcttcca tcacttgttt tttcagaaga atttgctgca cttgaactgg
    21781 catcgcttgc attacttgat ccgcatcctc ctaataaaaa caatgctgaa cttgccagca
    21841 accctgtaaa caataatttt ttcatatttt acctccaaat tttaatgtaa ccgatgtcgg
    21901 ttttaatata acgatatcat tttgttctga aaataacaat ttataaggta tgattctaaa
    21961 aaaattatta atgataccaa tactttatat aattcgcaag cgttttcttt gaatttttta
    22021 atctttcgta gcagaatgag aatgtattaa aaatagatta ggaggaattt tgatgtttag
    22081 acaccagaaa gaattgcagt ttgaagcaaa accagatcgt ccgaatccat taatcgcgaa
    22141 atatctgcag gagctgatcg gcggccaata tggcgagatg agtgtagcta tgcagtacct
    22201 gttccaaggc tggagctgcc gaggagaaga aaaatataaa gatatgctga tggacattgc
    22261 aacagaagaa atcggccacg tggaaatgtt ggcgacaatg gttgctcgtt tattagaggg
    22321 tgcgcctgtt gaagatcaag aagcagcgat gcaaaaagat ccaacaatcg gtgccattat
    22381 ctctggaatg aatccacagc acgcgatcgt agctggctta ggacctcgtc ctgctgacag
    22441 cgtgggcaat ccatggagcg gtgcctatat cattgcgagt ggcaacttgt tagcagactt
    22501 tagagctaat ttaaatgccg aatcacaagg aagacttcaa gtagcacgta tttacgaaat
    22561 gatcgatgat cgtggcgtaa aagatatgct gtctgttctc cttgcaagag atagctatca
    22621 tcaaaatcta tgggaatgtg cagtgaaaga attagaagaa aaagaaggtt caatcttagt
    22681 cccaagtact ttcccgcgtg acaaagaacg aaaagacatt gcttatgact tctataactt
    22741 ctcagaggga gaccaaagta gtgaaggttc ttgggctagt ggcaaagcac tggatggcga
    22801 aggcgaatat caatggttga aagaacctaa gatcgaaggc gctgcaccaa aattagaacc
    22861 agtaacacca aaaatgtatg ggacacctcc attcaagaaa taaatatcta cccgtagaaa
    22921 cacccgtttt ttgttccaca cgctttgtga gcaaaactga ctaaagtcat taatcataac
    22981 cacccttcaa aagggtggtt ttctctagag ctataagctc aaatcaccgg ccagcgccta
    23041 aagacgctgg ctttcatatt ttatgttcaa gccatattgc ctttgccagt tttgcccacc
    23101 tcttaaaagg tttatgtact actccttagc ccttaaaagg atcgtcttac tctcgtacac
    23161 tcagcttatc taacgcacta tcatgttttt cttgttcttg aatgtacttt ttgatggtcg
    23221 cttcattttg tcctactttt gtcacatagt atccttccac ccaaaagtgt cgatacccaa
    23281 atttatattt tagatttgca caatctcttg atgatctcct gaatattggc tcgatattga
    23341 ttataaatta tttttcttct atactttggt gtgaatacaa tgtggtattt acacatctac
    23401 tttgtacggg ctaaactatt tccctttatt gctccacccg catagcaagt ggttttgtat
    23461 ttcaatcgct cggcgcgctc aattaggtct aaagacaata aatctaaaaa cccgctgctt
    23521 cactgacaga aacaacgggt tttcagaaaa aaatgaaatg aaaaagatct cttacggcaa
    23581 tctattttat ttttttgatt ttttataaat ggaaaatgct tcaagaagtc caagaaataa
    23641 agccgtataa aaaccagaac ctatgaaatc tttatttatt agatattcaa cagaaatccc
    23701 gataacacta gccaacaata tatttatcag aagtgtaaaa taaggatgct ttttatagaa
    23761 tagaactagt gttgttttca tagactctcc tttcttcata tttccaatta atcacaaatt
    23821 cacggcatta ctagaaaaat taaaggggcg cagaagtgga aaaatactct tcgactttcc
    23881 ctacaaacca ggccatcttg tgtttaataa ctataccaaa tctaaggtac ctctaaatct
    23941 aaattccatt tgtggcggtg acctccaatt tttaactctt ctatctccca tccagtatac
    24001 ataaccttga tacttcgacc acttatatat cgaacacaca aatacctatc cattgcagga
    24061 gtactaccta acttagttgc tacttgcatc taaataacag ctgaaaagat aggacttact
    24121 cttagtaaag tatctgaact attaattttt aaactatcat ttttatcctc aactacatgc
    24181 acatctgttg caacttcact ttctgtctca tccagataaa tgattaagga atcgggaatt
    24241 gcaatatctt cagctgacgc tggaatagtt gagctaaatt agtcatcata tctttattat
    24301 aaaataacca acagcaaaaa atacaatgaa aaacaaactt ggaactaatg tatcattaat
    24361 aaatgttgaa aaattcagca actcattact gtatgatttt agcagcccaa ccaacatata
    24421 ccctataaaa gctaataaga tggttaggaa tcctttagaa tttttactca ttcatagaca
    24481 cttccttaaa taacttttat tatagaaggt attgtaaata ataatattga caaaaagtga
    24541 ctcaaaataa aaaaaacctt acattatact ggaatgaact gtattgatag tgtacctaaa
    24601 agctctactg acataagatc gtgtaatcgg taaagtaatt ttattaatta tacgggatgt
    24661 aatcaattag agaataaact aatcataaaa ggtactttta cattcataat tgtcgctttg
    24721 agattctact ttggttcact atctgaccca attgcacatt ttcgatcatt acaagtattg
    24781 gtgtcgttag aacaactaaa taaaaactag cggtagattt ttcagaatct atgctaattt
    24841 ctgtatggat gttccagtga tggcaaattt ctcttttcat tgtttttctt gtcgcatgat
    24901 atgccttttt gtgttattca gttaaactat agaaattctt tcctagacag atcttgatcc
    24961 caaattttac gtttttgagc atcgatatag tttaaaatga cattactcat atctaaagag
    25021 tttagatttt ttaggactaa aactgtcagt tgttcttttt cttctttctg ttaaagtatt
    25081 gtcgcgactt ttcgtatacg ttaagatatg tcgtgaaaaa acaagttttg tgacacaaaa
    25141 aaacgaatga cttgaggaaa tcacctcaat cattcgttta aaatgctata tattctttag
    25201 tttaagatac ggaaattaat gccatcgacc acactagtag aatgaacgac atttacttgg
    25261 ccttttttta atttcgttat gtttagcata taaaaatatt atagagttta cttacaatgc
    25321 ttaaaacata tactgtataa tctgatcaat tttagccatg tcttgcaatg gtaagttttc
    25381 tatttttttg agttttcgtt ccttaaaatc aagagatttt aattgtgtaa taattacttg
    25441 tccttttata tctagctctt caggtaatga gtaacgtgtc ggtaaattct ttatagtgga
    25501 agtaattgga catatgaccg aaaacattgt tttacggtta aattcataac gagagacgac
    25561 tagtcctggt cgtctttttt gaatttcttt tcctacggag ggatcaaaat caatccatac
    25621 aatatcacct tttttaggaa tataaattct ttcttctgac actataaaat ttctctccct
    25681 tcaggagtta aatcttccca ttcgtctttc tcataatact cggcctcttc cccaccacta
    25741 aatggatctt caatttttgg aactaacgta attgttccat catcagaata cacaacgata
    25801 tattcttggt tttctgaagg tttctttccg ttatcagaag gtaaagttac cactacagaa
    25861 cttccttgta atctagattt tgtaacaagc attatcatca ctcccttaat aaaagtatac
    25921 tatataacac taagtaacgc aaaattcccc tttgtaaact atctagaaaa ttgttttgtt
    25981 taaagtatat cttattggat ataaatagaa ataaattgta gccagttcat tttttcttta
    26041 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
    26101 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
    26161 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
    26221 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
    26281 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
    26341 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
    26401 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
    26461 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
    26521 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
    26581 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
    26641 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
    26701 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
    26761 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
    26821 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
    26881 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
    26941 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat aaaagccact
    27001 cattgggaaa acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac
    27061 tgtctctgca cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt
    27121 catgatttcc ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga
    27181 cggaaaaact tccccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc
    27241 tcgataataa ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct
    27301 aatccctccc aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac
    27361 gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg
    27421 tattctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct
    27481 ttttgaaatt gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac
    27541 ggtgttcaaa agcttgcatc tgcggtaata agcccaacca aacgaaaaat atgagcagct
    27601 atttttattt ggctgttctt attacttgat gcagggcagc tttttcacaa cctctccact
    27661 aaacggtgtt caatcagcta accttcggta ttagctcaaa aaacggcaaa atatgaggaa
    27721 ctattttgcc gtttgtttct ctaatactca ggtcaaagcg ctgatttcac aacctctaat
    27781 acacggtgtt ccagaagtag cttcttcgaa aaataagccg aaatttcaca aaaatttgag
    27841 aagcaatttt cgtaaattcc ttcttatttc ttgaagctga ccacttctgt cacaacctct
    27901 aattattaaa gaaccttttg cgaagttaac aatctttctt tgatcaattg aaaattctgc
    27961 ctaagtagtg atagatcctg tgtgactttc aacaataaga tacttccctg gatttcagca
    28021 atcaacgctt tggcttgacg gtctgcttct tcatcggaca gtttttcttc agaaagtgcc
    28081 aggatatttt gtttcgtata cttgacccat tgattgacta attctagtag caacagacgg
    28141 tattcttcat ccactgtact caactcaata atcaaattac ctacaggaca gccatgataa
    28201 gtgatctgtg tctcatgatc agcgaaaatc cagtccaaca tttcttctaa tgcttcttct
    28261 ggctgttttc cagcatttaa gatgtcttcg aacaattctg tctgccatac gtccatatgc
    28321 gcatggatca ccgctagaca aacttctttt tttgaagaaa agtaataata aaattgacct
    28381 tttcctgtat cagaagctgc tagaatatct cggattgacg tttcatgata gcctttttta
    28441 taaataagat ctttggtcgc ttccaagatc ttttcctttg ttttcaatga tattctcctt
    28501 tttcatttct catagtccta tattatacag taaattcatc attttttctt cttaatcgtt
    28561 taaaaaagac tgtgccaaat tatctgacac agccctcact ataaaactgt tctcatatag
    28621 ctagatgttc attgattcaa gaactgcgag gacaaaagca attcctatct tatgatagtt
    28681 gctctaatac ttcctctgtt gtcatcactt gatctgcaat aaagccaaag ttcgtcaacg
    28741 ctgcatccat tgcttcttgg ccaggagcac caattgcatc tgtcgctacc acaacattga
    28801 atccactctc tatcaattca cgcatatggg aatcgctaca caagtttccg ttcattccac
    28861 caagaattac tgtgtcgatt ccattttttc tcaattggag cgccaaatca ttgctttctg
    28921 gtccaaagat tttatgagga gagctgatga tcgtattttc atccaaataa ggtttcaatt
    28981 cttcgataaa atcggctcct gaaccttctt ccactgattc atattgactt tttctagcaa
    29041 acatctgctg ctcaagcatc atatgttctc ctgcaccgcc aaattgccat ttatggtcat
    29101 gtgggtaaaa ataatgagga gaaataaaga gtttgtatcc tttttctttt gcttgtttta
    29161 ccatttcaat catattttcg atcgtattgt atttttctaa gatatctttt gtcaaaccaa
    29221 agccaccgcc tgctggtttc aagaactcga cttgcgggtc agtgagaact actgctgtat
    29281 ttttattatt gaacatggtg gattctccat tcgttattta ttttaataca tcatttagag
    29341 cttcactcat tgtcggatga gaatagattt gatctctcaa taacgtataa ggaagttttc
    29401 cattcatagc tagcgaaatc aagttgatga cttcatgaga atcttccgca tagatacttg
    29461 cccctagaat caagtcagtt tcagggtcga ctaaaacttt gaatacacct ttaggatctt
    29521 ctaatacttg tgctttaggg acaccgcttg tcatatactt gaataatttg taattgacgc
    29581 cttgtgcttt ggcttgtttt tctgttaaac ctacattgga taaagtcgga gtgataaata
    29641 cactgtaagg cacagctttc cggttattcg ttgttcgttt attctcgccc ttcaactgat
    29701 ccataatgat tcgataatca tctagagaga tataagtgaa ctgcaatccg cctttgacgt
    29761 ctccaatcgc ccaaatgttg tctgcatctg tacgaaggta ttcatccaca gcgattgctc
    29821 cgcgatctgt tagtttaaca tctgtatttt ctaatccaag tgatgctgta cttggttttc
    29881 ttccagttgc agccaaaact tttgacgctt ggattgttac ttcttggcca tcttctgtaa
    29941 aggtgacggc agctgttgat tcttggtctg ctacttgatc tacagataca cctagatgga
    30001 attcgattcc cgcatttgtc atgtcttcta aaatcatttg cgcgatatct tcatcttcac
    30061 gtggaagaaa ttcagaatgg gcatcaagaa cgacaacttt agatccgtat tcattgaaca
    30121 ttgatgcaaa ttccaagcca atatatccag ccccgatgat cactaatttt tccggtaatt
    30181 gttcctgatc catcgcttgc gtagaatcaa tcaagtagcg acttgtattc aatcctttga
    30241 ttggtaaaat gattggttca gaaccagtat tgatgaaaat ccgttcgcct tcaacaatca
    30301 ctttttcacc attattcgtc acttcgattt gatggttaga aagaaaacgt gctgtaccgt
    30361 ccatcactgt cccagttgcc tcatcgctga tcatatgata atttttggcg cgaagcatgc
    30421 ccgtcaattt ttctttccga gcaacagcct ctgtaaagtc gattcctctt tctccattga
    30481 aaatcaatga tttagaagga atacagccga tgttgataca tgttccgcca tacatacgtg
    30541 ttgacttttc aacgactaaa accgattcac cttttgaagc taacgccttc gctaaagtct
    30601 taccgccctt tccaaaaccg atcacaatat tttgatattt ttccatcatt tttccctcgc
    30661 ttctttttta cctaccgatt ggtacaaaaa tatgataacc gataattgtc tattctccaa
    30721 atcatctgct ttatttgtca aaaaaaacca gacaaaaagc ggtcctttca cgtttttagt
    30781 ctggttcgat ttcctttatt cttctaaaca atcgttactt ctacatcgat atttcctcta
    30841 gttgctttgg agtaagggca gaattcatgc gcatcttctg caattttttg ggcttcttct
    30901 tcgctgatgc catcgatatg cacttctaat actacaccga tctgatagcc gccatcatct
    30961 aaattataaa gagagacacg tgcttttact gtggactctg ctgtcattcc tgcttctttg
    31021 atcatatgtt gtaccgcccc attgaaacaa gaagcataac ctgctgcaaa taattgttct
    31081 ggatttgttc cttctgcatg gattcctgga ggagtgattt tcatatgcat cgagccattt
    31141 ggtgcctcga cttctccatt gcgcccgcca tggttgatca ttgctgtttc gtatgctttt
    31201 ttcattgtac tgctcctttc gattgaccat ttgctacttg gctataatta tagtgtatga
    31261 caagtaaggc gctttctaca aaaattctgc ttattcgaat accagaatgc cgtcttctac
    31321 tatttttcca aaaaacaaaa aaatactact cgtgaacaac agcgtcatat ttgttccaaa
    31381 ataaatagca atcttttcta cataatcttt tatatgattg attttaagat acatatatac
    31441 cagaaagttg accccataaa ccaatactgt taaaaaaata accatattcg ttaccatttt
    31501 ttcctccttt tgctggtctc cctaccagca ctcgatttaa ccataccact ttttttgccg
    31561 tccaagcaac tagcctttca aaaagactgg tttattctgc tgttaatgat tcaagatcag
    31621 aaaaatgtcc gaataatttt gctaatttta ctaacgccaa cttgttcttt ccgaattcat
    31681 cattttctat ctgtaatact agtaacggct catgtaagct taaacggatc aaaaaccagc
    31741 cgtttccgta tggctcttct agatcatatc gaataccttc ttcattttcc gggtcgaagt
    31801 tcatcccttt ttcttcttcc aacagtcgct tcatttcttc taatatcttg tttccttgtt
    31861 cgcgtggttc atcgctgaac aattttaatc ggatttcttg tacttctacg ggctgtttca
    31921 actctttgat ttgttcatct aatgtgatac cttttctttt taattctggc aaaagcatca
    31981 ggatctttgc gatgacatat gcaccatcat ccagaaaata gttttccttg aatgcagcgt
    32041 gtccgcttgt ttcgatggct agctgtgtat ctttcccaag acggttaagt tctaatgctt
    32101 tattgattac atttcgatag ccggaaatat aacgaacttg gatgcctcct ttttcttcaa
    32161 tgaatctttg tagatgagaa gaagtagggg aattcgtcac gattcttgtt cctggatgct
    32221 cttttaatac gattgtactt aatactgcga tcaaattatt tcgattgatc actgttccag
    32281 aagcactgac aactgcagaa cgatccacat ctgtatcgaa aatgatccca agatctgctt
    32341 catttgctaa aacagcctgc ttgatacttt ccatcgcttc tttattatca ggatttggga
    32401 tatgattagg aaatcggcca tctggttcca aaaattggga acctgttgaa tccgcaccta
    32461 agctattcaa tactttttca gcaaagaatc caccagcacc atttccggca tcaacaatga
    32521 ttttccaacc atttagtggt ttgctttcta gtgttccaat ccccttttta atttttgtga
    32581 ctaaatcttg cgcataaggt gtcaaaaggt ctgctttttc tacgcttcca gtttgttcgc
    32641 tttttatttt ttgctgctgt gataggataa aagcaatatc ttctttttct gctcctcctt
    32701 ctgaggtaaa gaattttatt ccattataat aatagggaag atggctagca gtcagcataa
    32761 ccgcagcatc gcacgagaac tgttcaaatt gagtggacat aaacatagca ggcgtagtag
    32821 ctaattcgaa atcgatgaca tgtatccctt gttctttcag actttggatc aacgcctctt
    32881 tcagtgctgg tccgcttatc ctactgtcat gaccaatccc aatcgtccat tttttatttg
    32941 gatcggttga tttcttcttt ttctttatcc agtttgctag tccccagccg atctttttta
    33001 ccatgtctgt cgtcaagttt acttcatatc cttcaactgg taatgcgatt cctcgaatat
    33061 ccgatccatt ttgcagcgct ttcatatctt tcatataaaa gtcctcctca tttattccct
    33121 cattttttcc agcttttctg caaatcgctg tgcttcttct tctgagcggc aaataataat
    33181 gaatgtatct acaccggcca tcgttccgac aacttcaggg taagccactt catctagcca
    33241 attcgttacc acatcggcac catttcgttc agtcaatacg ataaccatga actgaacaga
    33301 ctggatacgc aaaacctcgc gtctaattgc tgtacgcaaa cgttcttcat ttaaagaaac
    33361 agatggctgg ttaaataatg tatagcgtac tattttgttt tcattttgac tttttactaa
    33421 cttcatttct cgaatgtctc gagaaatcgt tgcttgagtc acatcggcgc ctgcttcatg
    33481 cagccgcgca agcagctctt cctgtgtccc aataggttgt tctgtaatga tctggcggat
    33541 cagtgcttgt cgctgtttct ttttcatatt agacacctct caatgttgat tatagcaaaa
    33601 gataagtaat atttcttcat attttggtat tttttacttt tattttttgt tttcattcag
    33661 gttgctaacc tttttctatt ttaacacgca aaatcgtctt cattttttca ggtgctgatt
    33721 ttcttggatc gctgatgtaa atctctttgt gttctttaga agctctttca tagccttctt
    33781 ttgcgatgaa tgcttctaat ttctcaaacg ttgcaggttc ttcggcatag ggcccgacgt
    33841 gtaagatttg ggcacaaaga ccttcttcca tctttttgaa tactacttgt tctttcaagt
    33901 cttccggaag tttgctagaa cgctgaatgg cttcttgtgc tatcgcttct gtaacaaagt
    33961 caggctgtcg gatcatcaat tgataagaaa aatgttcttt tttcatcact ggttcatgca
    34021 aatacttttc ttgcagtccc cattgtcctt ccagtggata gacagtatac atctgataat
    34081 caggaatcag ccaatttttt ttaggcgaca tgcgtattgc atagctgact gcatatagac
    34141 aggctactct tcgtttaaag tcttcctgat tcgggtcgcc gatccctctg atgctaaaga
    34201 atggtttttt aggaacctct agtatttgcg ggcttttttt catactatag ctcttgtctt
    34261 tacgccattc aattttttcc atttttcttc ctccgcgaaa ttccatttag ttatttatta
    34321 tcatacctct ttttgagtga cttggctaat cgtgttgaca ctatttttca gtttgtgcta
    34381 gaataagtga agtaaataaa tcgtacagat aacacgtttc ggcgtgtcga gagagaggcg
    34441 gtttctattt ttataataga aatcactctc tttttttacg aaatttttta gaggtgatgt
    34501 aaatgacaaa acaaaaacag ctgcttttag tacctgcagt aattgcaaca gggatgatgt
    34561 cttttgcggg cgtattgatc gagacggcca tgaatgtaac tttcccaact cttatcagac
    34621 agtttgattt aacgactgca caagtccaat gggtaacaac gatttatctt ttgatgattt
    34681 cgatcattgt tccgctgtct acttatttga atcgcaattt tagtctcaaa aaactctttt
    34741 tagcttcaaa tctgcttttc ttggcaggta taatgatcga tttcttctct cctaccttca
    34801 tggtcctatt gtttggtcga ttgctccaag gtgcagcgac aggtatcgct cttccgttga
    34861 tgttccatat tatcttgaca tttgcccccc tcaatcgacg aggcgcgatg atgggtgtcg
    34921 gtactttgac aacggccatt gctccagcaa ttggccccac ttatggaggc attatgactt
    34981 cacattttac atggaatcat atcttcctgt ttcttgtgcc cgttttgatt ctttctttga
    35041 ttatcggctg gtttgctatt cccgagatgg cagtacaaaa aagtggttat ttggattgga
    35101 taagcgtttt aggaatttgt cttttgttta gtggaagtct aacatttttg agtacgttgg
    35161 gaacagtttc cagctggatt gctttaatca tcggcggtat tggctggttc cttttttact
    35221 atcgaagtaa aaaagtggag catccactga tccgactgtc tattctttca aataaaacat
    35281 tccgcttgtt tttgattggc ttcttagttt atcagttttt attacttggt gtatcttttg
    35341 tcttgccgaa tttccttcag atcgttcaag gaagtagtcc ttttgtcgct ggattagcga
    35401 tgcttcccgg cgcagcggtt ggtgctttgc tttctccttt ttccgggaaa atgctggatc
    35461 aatatggtcc gaagaagcca atttttgctg ggctgattct ttctttgatt ggttggctag
    35521 cattattcct actcatagga cacgcttcag tgggtgtatt gattgcttgt catgtgtttt
    35581 atatgatcgg tatcggatta tcttacagca atatgatgac caccggaatg aatgcactga
    35641 atgaagaact acaaggagac gggaacgcaa tgttcaatac cttgcagcag ttttctggcg
    35701 ctgtggctac ttcattagta gcggtcatta tcaattacgt ccagcatcat acttcccata
    35761 actatgaagt gtctactaca ttaggatcaa aagcagcatt aggcgtcttg ttgctgttgc
    35821 ttctcgtaag ctttgctcga ttcgcctatt atctgttctt tgctaaaaaa gcgtagattc
    35881 catatacggc aggtgttgca tctgccgttt ttttgtgtga aaatggttgt ttgtaaaagg
    35941 aggctttttt atggaaaaaa ttgccattat cggtggcggg atcattggta tgaccctagc
    36001 cagttactta gatacatcaa aattcaatgt tgctttattt gatgaaggga caggacaggc
    36061 aactaaagct agcgctggga ttatctcccc ttggttgtcg aaacgcagaa acaaacaatg
    36121 gtatcgtctg gctaaagacg gcgctgcttt tttccctaaa cttataaaag atttccaact
    36181 tgatcaaacg atttacgcac aatcagggac acttttgctt cgacctgaaa aagatttagc
    36241 tgaattggct gttcttgccg agaaacgaaa agaagaagcg ccagaaattg gaaacatcga
    36301 atggctttca cctgaacaga catctcaagc acttccgtta ttgaaaccag cagtttcact
    36361 gcgtatcagt ggtggtggtc gtctggatgg aaaagcatat ttagaacatc ttggtcagcg
    36421 tttagcacaa aaacaaatgg agcagatcca ggcaaaagtc actttgcata agaagagcga
    36481 ttcttggctc atcaagggac cgtttgaaga atacacagct gatcgtgtaa ttttgacacc
    36541 tggcccggca cttaagcctt tattacaaga attaggctat gctgcagata tacgtccgca
    36601 aaaagggcag ctcttagcat ttcagacatc ttataagatg agtcaagact ggcctgttgc
    36661 tatgcttgac ggagaagctg atttcattcc tttcatggat ggaaagatcc ttttaggtgc
    36721 tacccatgaa aacgatcaag gctgggattt ggctcctact agcgaagctt ataagggatt
    36781 gactaagagt acagaacagt tcttgactga tccagatcag ataaaaaact ggccatacga
    36841 gttccgtgta ggtacccgtg cttatacgtc agatttttcc ccattttttg gcgtactgcc
    36901 agatgatcca agcttactgg ctgctagcgg tctaggctct tctggcttga ccacaggtcc
    36961 ttatatcggc tatctattag ccaacttctt aaatactggt cattggtcta cggaagatta
    37021 tcaaaaggac atctccacct atattacgaa aaatccatca tagaaaaaca gctttttcaa
    37081 gtaaggatcc cgagcgatat tgcgcatgga ttacttcttg aaaaagctgt tattttagtt
    37141 caatttctga atcaaattct ccaaactttc ttttgcttca tctagcaaga gatcttttcc
    37201 tatctgagct tcaatgaatc gtaaacgaat atcaagtcgt tgtttcacca gatttttata
    37261 ggacgtatcc tcaaaagaaa caggaaaatc tggtataggt aaatagtgga tgtcttcaaa
    37321 tggaccaaac gacttgaatt ctgccgtttg ttgcacgatt ctttcaacga ttgacaatgt
    37381 cgtttcaggt ttgatttttt tatcatcgaa atatccagta ttcaggacat agcaggctgt
    37441 ttgctgtttc aaaaacaact ctcgaaaagc ttgatagtct tccctaagag gatagacacg
    37501 gaaaggatta gcaaatggtt caatgactag ctcatctagc tgtctttttg tagtgatatt
    37561 ttccgcgtct gatcgttttg ttgctaatgt tgcaccaaaa gacgctgcta aaactgggtc
    37621 attgatcttg ataattggcg gaagcgaatc atctttcatg atccaaaaaa cagcatcgat
    37681 cttttcgcta agatgatcga ctcggtcggg tgtcgcataa cgtgatttga ctgtccgccc
    37741 atttccgttt cgtatatctc ctgttactag gactttttct cctttacttg tacatgtcac
    37801 gcccacattc tgacaagtaa ggaaagaaga aatggccgga tcatcagacg gatagtcttg
    37861 tgttttatca aagtaagaag gttccaaggc agtagcaaaa ccattttttc gagaaataac
    37921 gaaagcatcg tcatgcaaga tcgaaacaga gtgaccttcc gtatgttttg ctaaggtaat
    37981 cgttgatttt ccagaaccgg acaacccaaa tacagccatc gtataggtct ggtcattact
    38041 agcatattgt ttcatcccac cgtggcaggc aataaagcca ttgcgatttc ccaatgccca
    38101 agcaagcgtc aaagtcgctt tttttaattc tccaaagtaa cgtaatccta gcacagccgc
    38161 tgtattatgg agagcatcaa acaaaatgag tccatttgga aatgtttcat gtgtccattc
    38221 gggatcacaa aagatataaa gatctccctc atcaaaggaa acagactttt gataagtctc
    38281 taagatcttc gaattcgctg gctgaaaatt cagcaaataa gaatacaaat tgttctcaaa
    38341 tccttctgga atcagcaaat gactttgcac agaaaattct tcagataggc caacgattac
    38401 ttttgtatgg taaaactggc gcttgcgact ttgatacaca gcttctctta aaattttctc
    38461 gtatttgctt tgttccttgt ctaagtcctc aaagatccgc cgagcacttg ctgttcttcc
    38521 cacaatgctt ccatcattga atactagtgt cctaacatca gctggtaaat ctagttcttc
    38581 agcatgtgag attgttaaat ctgtgatgat cgttcctgga ctcccttttg ctagttgata
    38641 agctgtcttc ctgtcaaaaa cttcatgaac attattaccg taaaatgtcg tttctacaat
    38701 cgatcgaata ctggataaca gtggatttcc acttgtgatt tcgtttaatt cgagactctc
    38761 ttttgtactc atggctcctc ctcattccct aaacaaaaaa acgttttctt tattatacat
    38821 tattcgagtt tttatttgta ctgctgcgct gtgggaagca aacgagttat ttttcacaca
    38881 aaaaaaccga gaacttgaat aaacaagttt ctcggtttca tcattttcct tttatcggct
    38941 tagtaccagc cgtttgctaa ccagaatgtt ttagcagcat cccatgaacc ataacgagat
    39001 gttacatatt gttctgctac acgttcttga ttggcagctg aatagtcgcc gtttaagtat
    39061 gaagcatcta attgataacg gccgatgtaa cgtccgttag tagctgtgta tgaaccgcct
    39121 gattcttttt gtgcgatcca ttcttttgca gagcttgaag ttggcgctgt tgcagcagct
    39181 ggggcagcag gagctacttc aactgtttca acaggtgcaa cgtatgtatg ttcttgcgtt
    39241 gctgtttcag ttgtagatgc tgtattttct gttgttttgt tttctgttgt tgctgaagaa
    39301 tcatttgaag ttgggattgt taattgttca ccaacataaa tgcggttgat gtttgcgatc
    39361 gaattttttt cagcaattgc atcgatcatt gagttgtctc ctgcaaattt ttgtgagatc
    39421 ttagataatg aatcacctga ttttactgta taaacttcat cagcgtgagc atttgtagtt
    39481 cctacaaaca ttgcagtagc accagcagca aaagttgtac ctaaaacaat tttcttaaat
    39541 gaattcatat taaactcctt tttttctccg ttttttattc tttcgacgct attaacttta
    39601 tcatttagaa atatttcata ggtaaaagaa acatgacatt taatgacaaa ataacgaatt
    39661 atttaatatt tgtaacattt aaacaaatat ttttttgata caaaaccata aaagccttgt
    39721 tgtataagca aagtaatcgt ttttctatgt ttttttattt tt
//
LOCUS       DAMYXM010000019        39234 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.019, whole genome shotgun sequence.
ACCESSION   DAMYXM010000019 DAMYXM010000000
VERSION     DAMYXM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.019"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(318..2699)
                     /locus_tag="PNS21_001160"
     CDS             complement(318..2699)
                     /locus_tag="PNS21_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDI5158879.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2689..3336)
                     /gene="recU"
                     /locus_tag="PNS21_001161"
     CDS             complement(2689..3336)
                     /gene="recU"
                     /locus_tag="PNS21_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDI5158880.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3400..3939
                     /locus_tag="PNS21_001162"
     CDS             3400..3939
                     /locus_tag="PNS21_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDI5158881.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4029..4448
                     /gene="gpsB"
                     /locus_tag="PNS21_001163"
     CDS             4029..4448
                     /gene="gpsB"
                     /locus_tag="PNS21_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDI5158882.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4470..4837
                     /gene="rnpB"
                     /locus_tag="PNS21_001164"
     ncRNA           4470..4837
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PNS21_001164"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5046..6212
                     /locus_tag="PNS21_001165"
     CDS             5046..6212
                     /locus_tag="PNS21_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDI5158883.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6258..7754
                     /locus_tag="PNS21_001166"
     CDS             6258..7754
                     /locus_tag="PNS21_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HDI5158884.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7835..8518
                     /locus_tag="PNS21_001167"
     CDS             7835..8518
                     /locus_tag="PNS21_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDI5158885.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9163..10353
                     /locus_tag="PNS21_001168"
     CDS             9163..10353
                     /locus_tag="PNS21_001168"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDI5158886.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10455..11408
                     /locus_tag="PNS21_001169"
     CDS             10455..11408
                     /locus_tag="PNS21_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDI5158887.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11477..12826
                     /locus_tag="PNS21_001170"
     CDS             11477..12826
                     /locus_tag="PNS21_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI5158888.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13009..13332)
                     /locus_tag="PNS21_001171"
     CDS             complement(13009..13332)
                     /locus_tag="PNS21_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158889.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13461..14624
                     /locus_tag="PNS21_001172"
     CDS             13461..14624
                     /locus_tag="PNS21_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDI5158890.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14694..16043
                     /locus_tag="PNS21_001173"
     CDS             14694..16043
                     /locus_tag="PNS21_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDI5158891.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16088..16519)
                     /locus_tag="PNS21_001174"
     CDS             complement(16088..16519)
                     /locus_tag="PNS21_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5158892.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16907..19549
                     /gene="alaS"
                     /locus_tag="PNS21_001175"
     CDS             16907..19549
                     /gene="alaS"
                     /locus_tag="PNS21_001175"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDI5158893.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19749..20345
                     /locus_tag="PNS21_001176"
     CDS             19749..20345
                     /locus_tag="PNS21_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDI5158894.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20467..21615
                     /locus_tag="PNS21_001177"
     CDS             20467..21615
                     /locus_tag="PNS21_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDI5158895.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21653..22066)
                     /locus_tag="PNS21_001178"
     CDS             complement(21653..22066)
                     /locus_tag="PNS21_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDI5158896.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22145..22576
                     /locus_tag="PNS21_001179"
     CDS             22145..22576
                     /locus_tag="PNS21_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HDI5158897.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22675..22908)
                     /locus_tag="PNS21_001180"
     CDS             complement(22675..22908)
                     /locus_tag="PNS21_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HDI5158898.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23091..24761
                     /locus_tag="PNS21_001181"
     CDS             23091..24761
                     /locus_tag="PNS21_001181"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDI5158899.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24908..25294
                     /locus_tag="PNS21_001182"
     CDS             24908..25294
                     /locus_tag="PNS21_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158900.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25281..25979
                     /locus_tag="PNS21_001183"
     CDS             25281..25979
                     /locus_tag="PNS21_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDI5158901.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25984..26448
                     /locus_tag="PNS21_001184"
     CDS             25984..26448
                     /locus_tag="PNS21_001184"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDI5158902.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26445..27356
                     /locus_tag="PNS21_001185"
     CDS             26445..27356
                     /locus_tag="PNS21_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDI5158903.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27591..28130
                     /gene="pyrR"
                     /locus_tag="PNS21_001186"
     CDS             27591..28130
                     /gene="pyrR"
                     /locus_tag="PNS21_001186"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDI5158904.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28144..29427
                     /locus_tag="PNS21_001187"
     CDS             28144..29427
                     /locus_tag="PNS21_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDI5158905.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29440..30366
                     /locus_tag="PNS21_001188"
     CDS             29440..30366
                     /locus_tag="PNS21_001188"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDI5158906.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30385..31665
                     /locus_tag="PNS21_001189"
     CDS             30385..31665
                     /locus_tag="PNS21_001189"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDI5158907.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31670..32746
                     /gene="carA"
                     /locus_tag="PNS21_001190"
     CDS             31670..32746
                     /gene="carA"
                     /locus_tag="PNS21_001190"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDI5158908.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32751..35936
                     /gene="carB"
                     /locus_tag="PNS21_001191"
     CDS             32751..35936
                     /gene="carB"
                     /locus_tag="PNS21_001191"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDI5158909.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35990..36766
                     /locus_tag="PNS21_001192"
     CDS             35990..36766
                     /locus_tag="PNS21_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDI5158910.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36763..37695
                     /locus_tag="PNS21_001193"
     CDS             36763..37695
                     /locus_tag="PNS21_001193"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDI5158911.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37692..38408
                     /gene="pyrF"
                     /locus_tag="PNS21_001194"
     CDS             37692..38408
                     /gene="pyrF"
                     /locus_tag="PNS21_001194"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDI5158912.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38415..39044
                     /locus_tag="PNS21_001195"
     CDS             38415..39044
                     /locus_tag="PNS21_001195"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDI5158913.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            39032..>39234
                     /locus_tag="PNS21_001196"
     CDS             39032..>39234
                     /locus_tag="PNS21_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158914.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRI"
BASE COUNT        12645 a         6926 c         8539 g        11124 t
ORIGIN      
        1 caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa tgggattatt
       61 aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta taaacaagta
      121 aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt taagctaaat
      181 gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat acagcttggt
      241 acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc acgcttgtag
      301 acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg
      361 tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat
      421 gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc
      481 tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta
      541 gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag
      601 ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct
      661 ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt
      721 gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac
      781 gattctgaag tgacaggtgt cattttttta ttgtaacctg tccacacgga aatcgaataa
      841 tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt
      901 gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg
      961 atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata
     1021 taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg
     1081 tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa
     1141 gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct
     1201 tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt
     1261 tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca
     1321 ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag
     1381 tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca
     1441 gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa
     1501 ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg
     1561 tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca
     1621 acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt
     1681 ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc
     1741 ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta
     1801 gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct
     1861 tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct
     1921 cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc
     1981 atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac
     2041 atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag
     2101 taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct
     2161 tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc
     2221 aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca
     2281 gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc
     2341 gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt
     2401 tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta
     2461 gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct
     2521 acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat
     2581 aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca
     2641 ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt
     2701 cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg
     2761 aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg
     2821 tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt
     2881 caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg
     2941 ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga
     3001 tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt
     3061 atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt
     3121 aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc
     3181 gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt
     3241 gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt
     3301 ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca
     3361 ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc
     3421 tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc
     3481 caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa
     3541 tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa
     3601 ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt
     3661 aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat
     3721 gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt
     3781 tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa
     3841 acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg
     3901 ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat
     3961 caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag
     4021 gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa
     4081 caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag
     4141 attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg
     4201 caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag
     4261 aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg
     4321 aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac
     4381 ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc
     4441 aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca
     4501 ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa
     4561 accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat
     4621 gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga
     4681 acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa
     4741 gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct
     4801 ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag
     4861 gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa
     4921 tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat
     4981 ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat
     5041 tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag
     5101 cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg
     5161 cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag
     5221 atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa
     5281 acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg
     5341 gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag
     5401 caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa
     5461 ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg
     5521 atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat
     5581 tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat
     5641 tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata
     5701 atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag
     5761 aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag
     5821 atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag
     5881 aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg
     5941 ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag
     6001 aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga
     6061 gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca
     6121 aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc
     6181 gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat
     6241 gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc
     6301 ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag
     6361 ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa
     6421 tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg
     6481 aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac
     6541 cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa
     6601 aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat
     6661 taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc
     6721 tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta
     6781 aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag
     6841 actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg
     6901 aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga
     6961 ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa
     7021 tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg
     7081 ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat
     7141 cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt
     7201 tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt
     7261 cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc
     7321 tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg
     7381 ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa
     7441 atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc
     7501 cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa
     7561 tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag
     7621 agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac
     7681 atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat
     7741 atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt
     7801 tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga
     7861 ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag
     7921 agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac
     7981 gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga
     8041 ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc
     8101 gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga
     8161 actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat
     8221 cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga
     8281 actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa
     8341 tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga
     8401 agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg
     8461 gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg
     8521 gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac
     8581 ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga
     8641 acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg
     8701 gcaggagaac accgtttatt cggaagaata ggactgtgac aagacttttg tcacagtcct
     8761 tttttcgaat tcaacaagta aaacttttaa gaaatattca gagagtaaaa ttaaaaattg
     8821 ctgataaacg aagatggaaa ttttatgtat ttttattggt ctatgcaata aataaattaa
     8881 atattcagaa tacaattgat tttaacgaaa gggcttctat caatggttgt gttgtattta
     8941 tctgcccagg tagagtaaaa tagatcatta tctaaaaggt aattgttcat actatataag
     9001 tcatttgaaa gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt ctcataatca
     9061 gtgtaagcgt tatcaaaaat atagcatact tgctttgtag atgaatgaca cgatatatgt
     9121 ttggtatgtt ctgcaagaca gatgaatatg gaggtggaga caatgtcttc caaagaacta
     9181 acaatgtatt tagagaaaga attggatcaa ctgaaagaaa aggggttata caatacgatc
     9241 gacatactag aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa gatgatcaat
     9301 ctagcatcca acaattatct tggatttgcc aatcgcgaag aattgaaaaa agcatgtatt
     9361 gaagcaaccg aaacttatgg tgtaggagcg ggagcagtcc gaacgatcaa tggttcgctg
     9421 aaaatccatc agcaattaga agaaaaaatc gcagaattca aagggacaga agcagcaatc
     9481 gcctttcaat ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat gacaaaagaa
     9541 gatgctatct tatcggatga attaaaccat gcatctatta ttgacggttg ccgcttatcc
     9601 ggagcaaaaa tcatccgaat taaacatcaa gatatgaaag accttgagaa aaaagcaaag
     9661 gaagcgatag aaagtaagaa atataaaaaa atcatgtata tcacagatgg tgttttctcc
     9721 atggatggag acattgcacg attgccggaa attattccga ttgttgaaaa atatggactg
     9781 atcacctacg tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc aggaaccgtc
     9841 aagcattttg gattaagcga caaaatcgat atgcaaatgg gcacgttatc gaaagctata
     9901 ggtgttgttg ggggttatgt agctggatcg aaaacactaa tcgactggtt gaaagctaga
     9961 agtcggccat tcctattttc tacttcgcta actccaggtg cagctgcagc tgcattggct
    10021 tcgattactt tgatgcaaga acatccggaa cttgtcgaaa aagtttggga aaatgctaat
    10081 tattttaaag aagaattgaa aaaagtaggc tataacatcg gtatgtctga aacacctatt
    10141 actccagtca ttttgggaga cgaaaaagtg actcagactt tctctaagaa gctgatcgaa
    10201 catgggattt atgctaaacc aatcgtttat ccaactgtgc ctcttgggac aggcaggatc
    10261 agaaatatgc cgactgcgga acatacgagg gaaatgcttg atgaagcagt agcagtttat
    10321 caaaaaatag gaaaagaaat ggaaattatt taactaaatt tagttttttc gtctatttaa
    10381 aattagagaa catgttcacg catatattgt taatcatctg aagatagtag taaaaaagat
    10441 cagggaggat catcatgaaa aaagtattgg tgacaggatg tttaggacaa attggttcag
    10501 agttgatcac acggctacgt caagaactag gcagtgaaaa tgtcattgcg acagatatac
    10561 gcatgcctga agtcattgag aaaaacgata tctttgagat cctagatgta atggattacg
    10621 aaaaaatgcg cagcatagca gaacaatatc aagtggacac attgattcat ttggctgctc
    10681 ttttgtcagc tgttgcagaa gcaaaaccac aattagcctg gaatttgaac gtgactgggc
    10741 tggtcaatgc attggaagtc gctagagaat tccagctgaa attttttacg ccaagttcaa
    10801 tcggcgcgtt tggtccaaat actccaaaag atcatacccc acaagatact cttcagcgtc
    10861 ctacaaccat gtatggggta acgaaggtag ctggagaact actatgtgat tattattatg
    10921 tgaaatatgg cgtagatact cgtggtgtcc gtttccctgg attgatttct tatgcaacct
    10981 tgccaggagg cggtacgaca gactacgcag tcgatattta ttattcagca atcaaagaag
    11041 gaaagtacac tagtttcatt gatcaaggta catcaatgga tatgatgtat atgccagatg
    11101 cgatcgatgc cattataaaa ctcatgaatg cagatcctga tcgattggtt catcgaaatg
    11161 ccttcaatat atcggcgatg tcttttgacc cggaagagat caaagcatct attcaaaaaa
    11221 tcataccaga ttttgaaatg gattatgatg tggatcctgt acgtcagtca atagcagaat
    11281 cttggccaaa ttcactagat gtttcttgtg cgcaagagga atggggcttt tcgccgaaat
    11341 acgacttaga tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa ttggtagaga
    11401 cggtataaaa caagaaccac atccaaaaag ttttttatac ttttgggatt ggctatggta
    11461 tgaaagggga caagggatgg aagaatatca agccacgcca gtaaaagaag taaaaataga
    11521 aaatgaatta aaacgtacga tgggattttt tactgcttta tcgactgtta tgggcacagt
    11581 gatcggtgca ggggtatttt ttaaagccgc aagcgtagca gaagttacag gatccgccag
    11641 tttgcatatg ttttcttggt ttctcggtgg tatgatttct gtctgtgcag gactaacagg
    11701 agctgaactg gcagctgcta ttccagaaac aggagggatg attaaatata tcgaacggat
    11761 ttacggaaat acagcagctt ttttattggg ctgggcgcaa gtcgtgattt attttccagc
    11821 aaatgtagca gccttgtcga tcattttcgg tacacagttc gtcaatctat ttggtctttc
    11881 ccagtcaatg attgttcccg tagctgttac agctgctgta tcgatcctgt tgatcaattt
    11941 tttaggttct aaagctggag gggcttttca gtcgattacg cttgtctgca agcttattcc
    12001 tttgtttgtg atcgtcattt tcggtttgtt ccgacaagaa ggagtagact tccaactgtt
    12061 tccgatccaa gcaggagaaa atctttcatt tttctcagcc ttgggagcgg gcttgctagc
    12121 aacgatgttc gcatacgacg gttggataca cgtaggaaat atttcgggag aattgaaaaa
    12181 gccggcgaaa gatttgccaa aagcaatttc tttaggaatc atcggaatca tgatcgttta
    12241 tttattagta aatgcggtgt tcttaagaac cgcatctatc gacggagtcg caggaaacag
    12301 caatgcggca agtgacgttg caaaaatgat ttttggcggc ttcggtggaa gactggtcac
    12361 agttggtatc ttgatttctg tttatggaac catcaatggc tataccctta ctggaatgcg
    12421 tctcccttat gttatggcaa aagaaaacaa cctgcctttt agcaagctct ttgctaaact
    12481 tcatgataaa actaaagttc ctgtagccgc aggtatctta gagctagtca tcgctattgg
    12541 catgatgatg atcggtggtt ttgatacact aacggatatg ctgatctttg tcatttggat
    12601 tttttatacg atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac ctgatttgtt
    12661 tagaccatac aaagttccaa tgtatccgtt tattccacta gtagcaatca ttggagggac
    12721 ttttatcgta tcaagcacgc ttatcactca gaccttttta gcttcgatgg ggatagtatt
    12781 gacacttgct ggtatcccga tttatctcta tttaaaacgt agatagctaa aacattcgaa
    12841 ttttattaga actgcacttc tctgccaaac aaaatatttt atttaaatga ggagtacgtg
    12901 gaagaaatgt caaaagtact cgttaatttt gatcacgtaa atgtcgtttt gtctacgatg
    12961 aaatagcaaa gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt actttgattt
    13021 gcgtaactga actattcggc gtacgccgcg ataaatattt aataaaacca agcaaccaga
    13081 aatgaaaatg ggaagccaaa tcagatagac agggtagcca agcactttag acaaagcatg
    13141 tggagtcatt ttatccattt caagaagtgc ttgctgccaa gtctgaagat tgaagactag
    13201 catgaaaccc gaaataatca ggagaataaa tcctaaataa tgaattgtag ttctttttct
    13261 tacataagcg atcgtagcat aaaggaacag taaaaaaata ggtacgccaa aaacaaagta
    13321 catattcagc atctcgacac cccctttgtg tttatcttac tttactttga aagaaatgag
    13381 aaataatagc ctcgttactt gcttgaaaca gcaaaggact gctaagataa gagagataca
    13441 aagaatcaag gaggagtcat atggcaagtt atatcgaacc gattcgtttt gcgctgattg
    13501 tttttccatt tttagcctta gcgatctcat ctgttttttt tatttatgaa tatcgaaagt
    13561 atggaacatt tctagtaaca agggcagtta ttttgtattc tttcgttttt tatttattgt
    13621 gtgcgtattt tttggtgatc ttaccacttc cggcaaagga gacggttgcc caaatgacag
    13681 ggccaaagat ggagctgcat ttatttgctt catggcaaca tttcagagac aacacggtat
    13741 tggtcttgtc tgaacctagt acctacctgc cggcaatgaa gcagagtgta tttttggaac
    13801 cagcttttaa tgttttatta ctcataccat ttggcattta tttaagatat tatttccggc
    13861 tttcttttat gaaaacgatg attgctagtt tctgcctttc tctatttttt gaattgacgc
    13921 agctaagtgg actttatttt atctacccaa gaccataccg cttatttgat gtgaatgatt
    13981 tagtgacaaa tacattgggt ggaatcattg gttttgtcgt tacgccgtta tttacattta
    14041 tgcttcctac aagaagacga atggatgaac tttcttatga aaaaggaaag actgtctcat
    14101 tgactcgacg cttggtcgct tatttgatcg actggacatt tttatctctt attaatttgg
    14161 tagcggcgat tttctttaaa attgcgttcg attcaggggc agttacttct catatctggt
    14221 ggcttttatt agaagtaggg gtatacttta tgattgtacc gtttataacc aatggatgga
    14281 cacttggcaa aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa cgaatttctt
    14341 taaaagcatt gatgatccgc tacgggtatc tgtactttct tttttatggt ttaagctggt
    14401 tggggcagtt gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg cagatgagtg
    14461 cagtattcat ttctctcggt gtcttgttac tacaaggtat ttttatttta aatatcttgc
    14521 tatcattttt gagaaaaaaa cggattctgt tttatgaaaa agctagtcac acatatactg
    14581 ttagcacgat tggccaaaca aaggatgatg attctcgaaa ttagctgagc aaaaacgctt
    14641 tgtctgtgtt ataatgccaa agtcgtgata atttaaagag aagaggtgca cgaatgaata
    14701 attttgatat attccgcttc aaaccgttta tcaatgaagc gttaaccgca aagggcttca
    14761 aacagcccac agaggtccag gaacgtttga ttcctttaat tgcaaaagga aaaaatatca
    14821 ttgggcaatc tcaaactggt acaggaaaaa cacatacgtt tttgttgcct ttgtttgaaa
    14881 aaatcaatgc agaaaaagag gaagtacaag ttgttattac agctccaagt agggaactag
    14941 ctgcacagat ctatgaagca gctacacaaa tcgctaaatt ttctacaccg gagatccgtg
    15001 tctcgaattt tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg catcaccaac
    15061 agccacaggt tgtagtaggc acgcctggta gaatattaga catgatgaat gaacaagcgc
    15121 tgaagattca caccgcattt gcatttgttg tcgatgaagc ggatatgaca ttggatatgg
    15181 gttttttaaa tgaagtagac caaattgcaa gtcgattacc agaaaagctg cagtttttag
    15241 ttttttctgc cactattcca gaaaaacttc gtccattctt acgaaaatac atggaaaatc
    15301 caatcattga agaaatcaaa ccaaaagcag ttatttctga agacattgaa aattggctga
    15361 tttcaacaaa aggtaaagat gagaatcaaa tgatttatca actgctgaca attggtcatc
    15421 cttatttagc aatcgtattt gccaatacaa aacagcatgt ggatgaaatt gccgattact
    15481 tgaaaaacca aggcttgaaa gtagcaaaga ttcatggaga catcacccca agagagagaa
    15541 aacgagtgat gcgacaagta cagaatctgg agtatcaata tgttgtagcg actgatttag
    15601 ctgctagagg gatcgatatc gaaggtgtct ctcatgttat taatgcagaa attccagaag
    15661 atctagactt ctttatccat cgtgtcggac gtactggacg taatggctta aaaggaacgg
    15721 ccattacgtt atattcgcca aatgatgagc aagccattga tgcgatagaa aaattgggcg
    15781 tctcatttga accaaaagaa atcaaaaacg gggaaatcgt caaaacatac gatcgtaata
    15841 gacgtgccaa acgagaaaaa tcaaaagatg tattagatcc cacgctgatt ggtcttgtga
    15901 agaagaaaaa gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg gcaattgcag
    15961 aaaaaaataa aaaagaacgc aaaatcgaac gcagacagca aagccgtact gctcgtaaaa
    16021 ataagaaaaa ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt gtcacaatct
    16081 cttgttttta agctagactt tttttcattc cgatacaaag tacttcatct tcaataaaag
    16141 gttctgtgca agtatgatac ccttgttttt cataaaaatc tacagccgtc atttcagcta
    16201 ctacataact agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct tcaagcaact
    16261 gaccgcctaa cccttttcct cggtattctt ttgctacgca aaatcgttcc ggcttgaagc
    16321 aagtaggtgt tttcttttca taacgcaaag tagctactgg tcttttttga tcaagaaata
    16381 gataataggt ttgttgcggg atatcttttc cgtcgaattc tagttctgca ggaatccctt
    16441 gttctaatac aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc cattcatccg
    16501 ctccaactat ttttttcatc atattcctcc ttgtcatgaa tagcagagat gtattgattc
    16561 ctttattctt atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga aaataaaaat
    16621 tgagatattg acagtgatca atagaatgcg tatactaatt aagtacgaaa aggatatgat
    16681 ttgtttttta agactcacag aaaattcttt cttggctgaa aaaagaatag tcactaatca
    16741 aattgctccc tttttctatt ttggtagaaa taccgacgca gctcagcgtt atcgagttta
    16801 agaggtaatg attacacatc attgcaatca aggtggtacc gcgagttttc gtccttggct
    16861 gtgatggtgt ttttttgtat ttaatttata gaaagcaggt aatgacatga aacaattaag
    16921 tagtgcacaa gttcgacaaa tgtttcttga tttcttccaa tctaaaggac atacgattga
    16981 acctagtgcg tctttgatcc ccgtgaacga tccaacctta ttgtggatca actctggtgt
    17041 tgcaacattg aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa gaatcacaaa
    17101 tgcccaaaaa tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag cacggcacca
    17161 tacgatgttt gagatgctgg gtaatttttc gattggtgac tatttcaaaa atgaagccat
    17221 ccactgggca tgggaatttt tgactgatcc aaaatggatg gcattcgatc cagaaaaact
    17281 ttatgtaaca gtttatccaa aagatacaga agcaaaacgc atctggcatg aagaagttgg
    17341 cctgccacta gatcacatta ttgatgtaga agacaacttc tgggatatcg gtgcaggccc
    17401 atgtggtccg gatacggaaa ttttctatga ccgtggagaa gcttataacg atttagcaga
    17461 agacgatcct gaaaactatc caggaggaga aaatgaacgt tatttagaaa tctggaactt
    17521 agtattttca gaattcaacc ataagcctga taacacattt gaaccacttc ctcataaaaa
    17581 cattgatacg gggatgggat tggaacgcat ggtctcaatc gttcaagatg cgtcaacgaa
    17641 ctttgagaca gacttgttta tgcctattat ccacgccgta gaaaagctaa gcaaacaagt
    17701 aaaatatggt gaaaacccag taacagatat ttcttacaaa gtaatcgcag atcatattcg
    17761 cgccctttca tttgctattg gcgacggtgc attgccatcg aatgaaggga gagggtatgt
    17821 cttacgccgt ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta ttgaacaagc
    17881 attcttatat cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt atcctgaagt
    17941 acttcaacaa aaagaattta ttgaaaaagt tatccgtaca gaagaagaac gtttcaatga
    18001 aacaatcaat gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa aaaacgaaaa
    18061 cgggacgact ctagcaggaa aagacatctt caaactatac gacacatatg gcttcccggt
    18121 agaattaacg gaagaggtgg cagaagatga aggactgaaa gttgatcatg ctggttttga
    18181 aaaagaaatg gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg aaaaatcaat
    18241 gggggtacaa tctgctttat tgacagatat caaagtagaa agtaaattca ttggatacac
    18301 agaacaagaa tcagatagtc aattattggt catcgttaaa ggtgagtctc ttttgaatga
    18361 agtcgcagaa ggtgaagctc aaatgatctt tgatcaaaca cctttctatg cagaaatggg
    18421 tgggcaagta gcggatcaag gatggatcat gaatgaagta ggcgaaatcg tagcagttgt
    18481 tacagatgtt caaaaagcgc ctaatggtca atttatgcac accgtagaag taaaagcacc
    18541 aatgagtgaa ggacaaacct atcatctatt gatcgatcaa tcgatgcgca atcggatcat
    18601 caaaaaccat acagcaaccc atctactgca caaggcattg aaagaagtac ttggcgagca
    18661 tgcaaatcaa gctggttctt tagtcgctcc tggacattta cgattcgact ttacccactt
    18721 tggtcaaatc acttcagatg aattagctga aatggaaaga atcgtcaacg aaaaaatctg
    18781 ggaagctatc ccagttgaaa caattgaaac agatatcgat acagccaaaa acatgggtgc
    18841 aatggctttg tttggtgaaa aatacggaca tgacgttcga gtagtaaata ttggcggctg
    18901 gtcaatcgaa ctgtgcggag gcacacatgt agcaaataca gaaaacatcg gaatcttcaa
    18961 aatcgtttct gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg taacaagtaa
    19021 agaagcgtac gaactacttg aagaagaaga aaaacaacta aaacaagtag caacaattgt
    19081 taaatcacct caattgaaag aagttgtttc aaaagcggaa caattgcaac agcaagtaag
    19141 agagctacaa aaggaaaatg aacaattagc tagcaagtta gcgaatcaac aagctggtga
    19201 tgtattcaaa gatccagaaa cgatcaatgg aaaaacaatc attgcagcac aagtaaaagt
    19261 gaaagatatg aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa atatttcaga
    19321 tattcttgtt ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg caatgacgaa
    19381 agaatcaaat gaagctggac taaaagctgg cgacttgatt aaagcaatcg caccgaaagt
    19441 aggcggtggc ggtggtggac gtccagacat ggcacaagct ggcgggaaaa atcctgctgg
    19501 tattccagat gcacttaaag ctgcgaaaga atgggttgcg caacaatagg gcgtttacgt
    19561 ttgacagaaa aacaaaagtt caaacattga acgaaacatg attatggggg cgaagacaaa
    19621 caagtcttgc cctcatactt ttttcaattg gatggcgcat ctaatcaaat ggtcggattc
    19681 ggatggtctt tagttgacga aagtgacaga aatttttacg attagtataa ataaaaaggg
    19741 gtgaaaaaat gaatagtaga aataagacct ttcgactagt cttacgtgcg atcttgttag
    19801 caatcatcat cgtacaagcg atggttcctt ggctggggtt tattccatta ggattcatta
    19861 gtttaacaat tatccatatc acagtaatta ttgctgcagt agtattaggc ccaaaagatg
    19921 gcatggtgat tggcttgttt tggggaatcg ctacaattgt tcgtgcctat gccatgccaa
    19981 caacgccatt tgatacatta gtgttcacga atccgattat ctcggtagtt cccagagtac
    20041 ttgttggctt agtagcaggc ttggttttcc actggattta tcaaaaaaat aaatcaataa
    20101 cgatcagctc agcctttgca ggggtactgg gttcattggt caatacagtc ttagtgttag
    20161 gattcatggg attattttat acaggtgcaa cggcagatgc ctatggtgta gactctgctc
    20221 ttttatttaa aaccttagca ggggttgcag caatcaatgg gataccagaa gcaataggtg
    20281 cgggaatcat aacaccatta ctagcaaaag cgttatttgc agcaacaccg ttgaaaccag
    20341 aataatgacc atcttttaat agtaatagga cgcacgatag atagaaaaag gaagagagga
    20401 gaggaaaaca atcgtcctct ccatcttttt acctgagcaa caagcaaact agaaggggta
    20461 aaaaacttgg ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca gcggacaaaa
    20521 aaaggagtac tagcgggtat cttaggattg gttacgaatc tgatcttgtt tgtctctaag
    20581 ctaatgatcg ggcttttttc aggaagtgtc tccattatgg ctgatgcagt gaacagtcta
    20641 tcagatactg cttcatccat attgactttg atcggattta aaattgctgc aaagccagca
    20701 gatcaggaac atccttatgg acacgaacgc ttcgaatata tcagtgggtt atttgtttcg
    20761 atcatcatta cttatgtagg atttcaattt ttagagtctt ctgctgaaaa aatcatccat
    20821 ccagaaaatg tctctttaac acccatcgtc ttttttgtcc taatcatttc catctttttg
    20881 aaatttgcac aaggcaaaat gtatcaggtt attgcgcaat ccattgattc tgaaacattg
    20941 agagctacag caacagatag ttataatgat gtctttacta cactggctgt attgctttca
    21001 gcagcaatcg aatgggtaac tggatggcgt attgatggat atataggttt tgctttagca
    21061 ttatatatcc tttatagtgg attgacgatg cttcgaaatt ttgtatacga actattagga
    21121 agtcgcccaa cgccagcaga gattgaagaa atggaagagc atttaaatgc ttgcgatgat
    21181 atacttggct atcatgattt attggtgcat aactatggac ccaataaacg ctttgcatca
    21241 gtccacatcg aagtagatga atcaatgaat ttgaatgtgg ctcataagat tattgatctg
    21301 atagaaaaag actttaaaaa gtctttggat gtggaacttg tctgtcactt ggatccaatg
    21361 cctgttgata acgaaaaata ctataatatc ttgcggcagt taaagcaaat tcttcttgaa
    21421 acaggtacag ggcttaatat tcatgatttt agagtaattc atcaaggaaa aacgctgcaa
    21481 tttgatgtag tggtccctga gaaattccag tttgttgata cagaactaga aagaatctta
    21541 cgtagtcaaa tagaagaaaa aatcggagat taccaattgg aaattacgtt tgatcacaat
    21601 tatcttttat attaaatgag aaactataaa aagactcctt aaagttttta atttaaagag
    21661 tctttttttc ttttaattgt ttatggagag cctgacgtgc aagattatct gctcctttat
    21721 ttttgttttc gggcaaccat tgcaggatca ataaagaaaa ttcggataga agttcttgga
    21781 ttctttgaag gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg accgtagaaa
    21841 ctagtgtttt gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg tttttcttta
    21901 gaaattctaa agtttttaaa aagacttcga actctgcttg atggtttgtc cctatagaaa
    21961 gcgggataga tagctgttct tgtttacctt gatagacaat caagattcca cctccactag
    22021 gaccagggtt gccttttgta gaggcatcaa tgtatacttt taacataaaa agagattctc
    22081 ctttttgttc aaactaatgt tatggtaaga ttatatcatt actgaatcgg tgagaggtta
    22141 gagaatgaaa aaaaatattc gatggcagcc tgaactggca catacgatca tctattggtc
    22201 attgaccttg atcattctgt tttttagtct gattctttct ttagaaaaca caggtcctta
    22261 ttggaaaagc aattttgttt taggtatttt tcttgttttt ctatttctag gtttgcggcg
    22321 ttcttttatt ttaaaatcaa actctgtgat tatacgttat gctgcttttt ggaaagaccg
    22381 agaaatatct gtcggacaga ttcgatcttt gacgccaact aatactgggg tgcaaatgat
    22441 gttgcaagat gagaaagccc catctgtttt catgatgcga aaaaaagatc aagtagtcct
    22501 ttttgatcat tttgaaaaaa acggacccaa atcagaatca attccttatc cttatacgaa
    22561 aaaaagaaaa agctaatttt aaaaatcaga acctaaaaaa acgaattttt acaggaaaag
    22621 aaaagagtga gacgtacggt tcatcccgtt tagcgtctca ctctatttgt ttatctattc
    22681 atgcatacta gtatcggtag tctgaacgtt gcctttcttg acatgggcag cttgtgttcc
    22741 tcgattccct tcgatgatct caaattcgac cacttgattt tcttccaaag ttttgaatcc
    22801 ctcttcttca atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat aactgataaa
    22861 gccgtatcct tttttgttgt caaaccattt tacaattcct gtggtcaaag cactcacgac
    22921 ctttctaaag tctgatcata gccttcatta taaagagtat ttataagtac gtcaaataaa
    22981 ttaaaaacct gtaattttat gttttttttc tgaataaaaa aaggtataat tggcatatct
    23041 taacaaagaa gatattttgt tgaataaagt tcgtgaaaag gggtaatcaa atgaagaatg
    23101 atctgcaaat ttcacaagaa acagagctag aaccgattgt tgaagtggct agtaaattag
    23161 atcttaagga agacgatttg gaactgtatg ggaaatataa ggcgaaaatc aatttttctg
    23221 gaatcaatcg atcaaaggat aacgctgagg ggcatttgat cttagttact tccatcaatc
    23281 caacaccagc tggcgaagga aagtcaacga tcactgtagg gttaggagac gcgctcaata
    23341 aaatcaataa aaaatctgtt attgcattgc gagaaccctc acttggtcct gtgatgggaa
    23401 tcaaaggtgg tgctgcaggc ggaggatatg cacaggtatt gcctatggaa gatatcaact
    23461 tgcattttac tggtgacatg catgctatca caacagctaa taatgcatta tctgctcttt
    23521 tagataatca tattcatcaa ggaaatgaat tgaacatcga tgcccgccga gtgatttgga
    23581 aacgggtagt cgatctcaat gatcgtgaac tgcgtaaagt aattgttggc ttgggtgggc
    23641 caatccaagg tgttccaaga gaagatgggt ttgatatcac agttgccagc gaaatcatgg
    23701 ctatcttgtg tcttgcggca gaccttgaag atttaaaggc tcgtcttgca aggatcgttg
    23761 tcggctatac atttgaccgt cagcctgtga ctgctggaga cttgaaagca gaaggcgctc
    23821 tggcattact gctgaaagat gcaatcaagc cgaatcttgt acaaacaatt tacggaacac
    23881 cagcttttgt ccacggggga ccgtttgcca atatcgctca tggctgcaat agtattttag
    23941 ctaccaagac agcattgaga ttagcagatt acgttgtcac agaggcagga tttggtgcag
    24001 atcttggcgg agagaaattt ttagatatca aagtacctaa cttgaaaaaa gcgccggatg
    24061 ctgtggtgat tgtcgccact gtccgtgcat tgaaaatgca tggcggaatg aaaaaagatg
    24121 aacttaaaaa tgagaatctt gatgctttga aagtaggttt tgcaaattta aagcgccata
    24181 tacgtaatat ggaacaatat cagctacctg tgatcgtcgc aatcaatgaa ttcgtaacag
    24241 atacagacaa tgaactcaca ctgttggagc acttgtgtga aaatcagggg attcttgcca
    24301 agagagcgag tgtatgggca gatggagcag aaggcggcgt tgatttagca aaagctgtag
    24361 tacgattgat tgatagaaaa gaagctaatt acaagccatt ataccgccta gaagaaacga
    24421 tacaagaaaa gacagaaaca atcgtcaaga aaatttacgg aggaaatggt gttgttttca
    24481 gcaaaaaagc gcaaaaacaa atagaagagt ttacgaagaa tggatgggac aaactgccga
    24541 tttgtatggc aaaaacacag tattcatttt ctgatgatcc ttcactgcta ggtgcaccgg
    24601 aagatttcac catcacgatc cgcgaaatca tcccaaaatt aggggcagga tttttagtag
    24661 ccttgactgg agatgttatg acgatgcctg gtttgcctaa aaaacctgct gcattgaata
    24721 tggacgtgac aaatgacgga gaagtgttgg gattgtttta agcttgatta taatctacca
    24781 tcgaaggagc tgtattttga ctacagcttc tttttttaga cgatagggaa ttaattttgt
    24841 ttacactagg atcattcttt ttctttattg tattttgagc tatactaaag cataatagga
    24901 gggtatgatg aaaacgacat ttataagaaa aaccggtttt tacggtatgg gtagctcgat
    24961 tcagctaaga atcaacaatg aaaaacgcag tgttctcagt catgaacaaa agataacgct
    25021 tgatctggaa gtgcccttta cgatgcaggt caccttttat tggctaaaaa gccccgttta
    25081 tacagtgaaa gaagcaaagc ctgcttatgt gatcacaatg aattacttga ttcttcaaat
    25141 ctatccgttt ttatttctag caactgggtt gggggccgtt ttcctacaaa atttattttt
    25201 cagttttttt gcagttattg tgttgattgc gttctttttc tatataaaaa accgggcata
    25261 tttgatcaag gaggaaaatt atggcaaact ctgatctttt tttaatcagt ttcaatcgaa
    25321 tcgaaaaatg gatgagagac gagatgggaa atgcacgaaa tatgggattt accgaacttg
    25381 tcagacgcct agctcaaaga aaacaattaa tgatcagaaa atacgaagat gatcttttac
    25441 agcttgctca gttaaggaat gcgatcgtcc atgatcggat cgcagtcgat tttgtcattg
    25501 cagagccaaa tgattggaca gtcaagcgaa tccagctgat cgaacaagaa ctgattcagc
    25561 ctgaaaccgt gctgcctcga tttgcaaaac gtgtaacagg ttttgaacaa gatcttccgt
    25621 tacttgaact gttaaagata gtagcagaaa aaaggtattc acaatttccg ttatacaata
    25681 aaggaaaatt tgtagcattg attactttac gtaatatcgg gttttggcta gcaaaagaaa
    25741 gccaaaaagg tccggttgat ttaacaaata aacgagcgtt ggatttgatc atccaaaacg
    25801 gaaagtacac aaattatcat tttgttccag ctaaaacaca tatttacgag gttgaagcga
    25861 tgtttcgtga gcaagggact ttggaagccg tacttatcac taaagatggg aatcccgatg
    25921 gtaatctgct cgggatcgta cgacctcgtg atatttataa acaaatagaa aaggattgat
    25981 tttttgttaa ttatttattg gatattcagc gctttgctag taggagtgga tcagttggtc
    26041 aaatggtgga tcactgataa tctagcgctt ggagagacga aaaatcttat accaggtatt
    26101 ttgtcactga atcatattcg aaacactgga gccgcatgga gtatgctaga agggaagatg
    26161 tggttcttta ctatagtgac actcattgca gtcgttgtga ttctcacatt gatgattaaa
    26221 aatcgtgaaa aaggtaatcg ctggttcatg attggattga gtttgattct agctggtgcg
    26281 attggcaatt ttatcgaccg tattcgtctt ggctatgtag tagatatgtt ccagacagat
    26341 tttatgagct ttccgatttt taacgtagca gatgtgacac ttgtatgtgg agtgatttgt
    26401 atgttaattt atattatttt agatgaaaag gatcaaagaa aaaaatgaca gaacttaaaa
    26461 cgacgattca ccatgaatct ggtaggatcg ataaagtatt gactaattta ttcaccgatt
    26521 acagtcgttc gcaaattcag ctgtggctga aaaatggagc ggtttctgtc aatggacagc
    26581 cagtcaaagc aaattacaaa gtaaaacaag gagacgagat tactgtcttg atccccgaac
    26641 cagaaacatt agatgtggtc ccagaaaata tcccgttgac gatcgtttac gaagatgaag
    26701 atgtagcggt gataaacaaa cctcaaggaa tggtcgttca tccttcagca ggtcatacgc
    26761 atggcacaat ggtcaatgcg ctgatgtacc atatcaagga tctttctacg atcaacggcg
    26821 ttattcgtcc aggtattgtt caccgaatcg acaaagatac ttccggactg ctaatggttg
    26881 ccaaaaatga ccaggcacac gaatcattag ctcagcaatt aaaagataag acctctttgc
    26941 gcaaatatgt tgctttagtc catggagaga ttcctcacga aaaaggaacc atcaatgcgc
    27001 caatcggccg ttcgaaggaa aaccggaaaa tgcaggcgat aagagaagat gggaagtcgg
    27061 ctgtcacaca ttttactgta ttggaacgtt tcgaaggatt tactctggtt gaattaaaac
    27121 tagaaaccgg aagaacacac cagatccgtg tgcatatgaa gtatatcggt tatccgttag
    27181 ctggagatcc agtatacgga ccgaagaaaa ctttgaaagg caatggtcag tttctccatg
    27241 cgcaaatgtt aggttttacg cacccgacta caaacgaaaa gatgatcttt gaagcacctc
    27301 ttccagaagt ttttgaaaaa actttagaaa aattaagaaa aaacattgca ttttaattta
    27361 gataacggta tagtaattaa caacgaaagc ataaaactta atagaattcc tttaagttta
    27421 gtcccgtgag gctaagaagg gtgggagcaa ggatacgtat ggtgcgccat aagtagattg
    27481 ctcattgaca tagtatagct atcagtgtat cagtatacgc tcagtctaaa cattcctcct
    27541 gtcctcttag ggcaggagtt tttttatgca aaaaagtagg gggaatgaaa atgcaagcaa
    27601 aagaagtagt agatcaggta accatgaagc gagcattgac tcgaattact tatgagatta
    27661 tcgagagaaa ccatagtatc caagatatcg tattggtagg tatcaaaaca cgaggaatct
    27721 acattgcatc aagaattgcc gaacgcctga agcagctaga agatatcgat attcctgtag
    27781 gagaactgga tatcacttta taccgagatg acaaaaaaga aaatccagaa gaaccggaac
    27841 ttcattcttc tgacatccca gtttccttag aaggaaaaga agttattttg attgatgatg
    27901 ttttgtatac aggacgaacg attcgagcag caatggacgc agtaatggat tttggcagac
    27961 caagaaaaat ttcattagca gtcctggtcg atcgtgggca tcgagaattg ccaatccgtg
    28021 cagattatgt tgggaagaac attccaacat ctcgggcgga agagatttta gtagagatgc
    28081 aagagcttga tggacaagat cgcatcatga ttttgaaaga ggaagattag aggagagaga
    28141 aacatgtcgg agaaaaaagg atttcataat gatgacgtgg tgttagatat ccacgatcgc
    28201 ccaaccccag gacattgggt aggattaagt ttacaacact tgttcacgat gttcggagca
    28261 accgtgttag tccccatctt agtaggtatc gatccaggta ttgcgctagt cagttcagga
    28321 ctaggtacat tggtgtactt atttactact aaaggaaaga tacctgcgta cttaggaagc
    28381 agcttcgctt tcattgcagc gatgcagctt ttgatgaaaa cagatggtta tccagcgatt
    28441 gcacaaggag cagtcacaac tggattagtc tatttgattg ttgcgctgat catcaaaaag
    28501 atcggttcag catggctgga taaaatcttg cctccaatcg tcgtcggtcc agtcatcatg
    28561 gttatcgggt tgggacttgc ggcaaatgca gcaacaaatg ctatgtataa tcagggcacg
    28621 tatgatttca agtatattgc tgtagcgctg atcacattag gattaacaat tttcttcaac
    28681 atgtggctaa gaggttttct cggattgatt ccgattttac taggtattat tagcggatac
    28741 atcgtagcct tgctgtttgg aatcgtggat actaagccaa tcgtagacgc tgcctggttt
    28801 gctgttccta actttgagat tccctttgta caatatgaac caaagctata tcttggagca
    28861 atcacaacaa tggcacccat cgcttttgtg acgatgacag agcatatcgg ccatcttatg
    28921 gtattgaata aactaactaa acgtaatttc tttgaagaac cgggtcttca taagacgttg
    28981 acaggtgatg gattagcaca aatcgtggca gcctttgtcg gaggtcctcc tgtaacaagt
    29041 tacggtgaaa acattggtgt tctggctatc acacgtgtcc atagcgtgtt tgtgatcggt
    29101 ggagctgcag tatttgcagt cattttagga ttcgtaggaa aattaagcgc tttgatttta
    29161 agtatccctg gaccagtcat ctcaggaatc agttttatct tatttggagt aattgccgca
    29221 agtggaatga agattttaat cgagaacaaa atcgattttg ataaaaagaa aaatctgttg
    29281 attgcttcag ttatcttagt agtaggaatt ggcggactcg tctttgaagt aggcacattt
    29341 acattgtcgg caatggcatt agcaactgta cttggcatca ttttaaatct tatcttacca
    29401 gaaacttcac gaagcgaaga aaactaagga ggaaacatta tgatcatcaa atcagaacgt
    29461 atcagcttaa agcatttatt gaccgttgag gcacttactg atcaagaagt tatgggcttg
    29521 atccgcagag ggcaggaatt caagcaaggt gctaattgga ctccgcaaaa gcgccagtac
    29581 tttgctacaa acctcttttt cgaaaatagt actcgcacac ataaaagctt cgatgtagcg
    29641 gagaaaaaac tagggctaga agtgattgaa tttgaagcaa gcacaagctc tgtccaaaaa
    29701 ggggaaacac tatacgatac agtactgacg atgtcagcat taggcgtaga cgtagcggtt
    29761 atccgtcatg gtgatgagaa ctattatgac gaactgatac aaagtaaaac aatccagtgt
    29821 tcgattatca atggaggaga cggaagcgga cagcatccaa cccaatgctt gcttgatttg
    29881 atgaccattt atgaagaatt tggtcatttt gaaggtctga aagttgccat cgtaggtgat
    29941 atcactcact ctcgcgtagc taaatccaat atgcagatgt taaaacgtct aggcgcacaa
    30001 gtattcttct caggtcctag agaatggtac gacgatgaat tcgaagtgta cggacattat
    30061 atgccattgg atgaactact agatcaagtt gatgtcatga tgatgcttcg ggtgcaacac
    30121 gaacgccatg atggaaaaga aagtttctca aaagaaggct accatcagga acatgggtta
    30181 acagttgagc gtgcgaagaa gttgcaaaaa catgcaatca ttatgcaccc agcaccagtt
    30241 aaccgtgatg tagaattagc tgattcatta gtggaaggat tacagtcgcg gatcgttcag
    30301 caaatgagca atggagtatt cgtcagaatg gcaatcttag aagcagtgct aaatggaaaa
    30361 gcgtaattat aggaggatga aaaaatgaaa acactcatta aaaacggtca gatcaataca
    30421 cgaaaaaata tgacgacacc agcagaaatt tggatagaag atgggcgaat caaagcaatc
    30481 ggtacaggat tctcagaagc cgaatttgat gaagtgtttg atgcaaaggg acaattgatc
    30541 acacctggtt tagtggacgt ccatgttcat ttgagagagc caggatttac atacaaggag
    30601 acgatcgaag ctgggactcg ctcagctgct cgtggcggtt tcactactgt atgcgcgatg
    30661 ccgaatttaa atcctgtacc tgatacggca gaaaagctga gacaagtata tgacatcatt
    30721 agaaaagatg cagttgtgaa ggtattgcag tatgcgccaa tcacagaaaa tctgcgaagt
    30781 gagaaattag ttgatcaaga agcactgatt gaagaaggtg cttttgcttt cacaaatgac
    30841 ggtgtaggtg tccaaacagc tgggacgatg tatctggcta tgaaagaagc tgcaaaaaac
    30901 aataaagccc tagtagccca tacagaagat gaatctttat tatttggcgg cgttatgcac
    30961 gctggcaaga aggcagaaga acttggtctt cccgggatct taagcgtaac cgagtcttct
    31021 caaattgcta gagatctgtt gctagctgaa gcaactggtg ttcattacca tgtttgccat
    31081 gtttctacta aagaaagtgt ccgtgtaatc agagacgcaa aaaaagcagg aatccacgta
    31141 acagcagaag tttcacccca tcatcttatt ttgatcgacg aagatattcc cgaagatttc
    31201 ggcttttgga aaatgaaccc gccgctaaga ggacgggagg acagagaagc tttgatcgaa
    31261 ggcttgcttg acggtacgat cgattgcatc gctacagacc atgcccccca tggattagaa
    31321 gaaaaaagcc aaagtttcat gaaatcacca tttggtatcg taggaagcga aacagcgttt
    31381 caactgatct atactcattt tgtagaaact ggtcgattca cgttggaaca agtgatcaat
    31441 tggttggcag ttaaaccggc tgagattttt ggtttgaatg caggaacgct aacggttggt
    31501 gcccccgctg atgtagcagt ttttgatatt acacaaactt gcacgatcga taaagaagac
    31561 tttttatcaa aaggggagaa tacacctttt atcggctgga aagtaaaagg cgaaacacaa
    31621 atgacttttg tcaacggaaa acttgtgtgg cagaagggag aataaaagta tggaacgttt
    31681 gcttatatta gaagatggaa cagttttcga aggaaaggct tttggtgcag atgctagtgt
    31741 cttcggagaa atcgttttta caacaagtat gactggttat caagaaacta tcactgatca
    31801 aagttttaac ggtcaaatca ttacatttac ctatcctatg gtgggtaatt atggggtaaa
    31861 tagagatgat tatgaatcga tttctccaac atgcaaagga gtagtagtaa aagaacatgc
    31921 acgtgtagct tcaaactggc gaaaccaaat gacgctagat tcatttttga aacaaaaagg
    31981 aattcctgga atatcaggta tcgatacgcg cgcccttact cgcaaaatcc gtgaagtcgg
    32041 aacgatgaaa gctagtatag ttgatgctgg agattccttc gaacatgcat ttgatcagct
    32101 gaaagcaacc gttctagcaa ccaatcaagt gcagcaagtc tcaactaaca aaccttatcc
    32161 aagtccagga acaggtcgga aagtcattgt cattgacttc ggcttgaaac atagtatttt
    32221 acgagaattg agtaaaagaa actgtaacct cacagtactt ccctataaca cagatgccga
    32281 gacgatttta gcgctatcac cagacggagt catgctgacg aatggacctg gtgatccaaa
    32341 agatgtgcca gaagcaatcg aaatgatcaa aaaaatccaa ggaaaagtac cgatttttgg
    32401 tatttgtctc ggtcaccagt tatttgcttt agccaacggt gcagatacct ataaaatgaa
    32461 attcggacat cgaggattaa atcacccggt acgagaagta gcgaccggaa gaattgattt
    32521 tacttctcaa aatcatggct atgcagtcga tgaagccacg attgatccag aaaaactcat
    32581 cgtaactcat gtagaagtaa atgacggaac agtagaagga gtcaaacaca gagattatcc
    32641 cgctttcagt gtgcagtttc atccagatgc tgcccctggt ccgcatgatg cactacattt
    32701 atttgatgaa tttatggaaa tgatggacgc aggaaaggag cagtaaacag atgccgaaac
    32761 gtacagatat caagaaaatt atggtgattg gatctggtcc gatcatcatt ggtcaagccg
    32821 cagaatttga ctacgcagga acacaagcct gccttgcctt aaaagaagaa ggatatgaag
    32881 tggttttagt aaattctaat cctgcgacga tcatgacgga taaagaagtg gcagatcaag
    32941 tatatatcga accgatcaca cttgaatttg tatcaaggat tcttcgaaaa gagcgtccag
    33001 atgctctcct tccaacttta ggcggacaga caggcctcaa tatggcaatg gagctggcag
    33061 catcaggtat tttagaagaa ttgaacatag aacttctagg aacaaagctt tctgcgatcg
    33121 atcaagcaga agatcgtgat ctattcaaac agttgatgaa agaattgaat cagccaatcc
    33181 ccgagtctga aatcgtcaat acagtagaag aagctgtaga ttttgcaaat aaaatcggct
    33241 atcctgtcat cgttcgtcca gcctttactt taggcggaac tggtggaggt atgtgtgaaa
    33301 atgaagaaga attacgtatc gtatcagaaa atggattaag tctttccccc gctacccaat
    33361 gtttgattga acgaagtatt gcaggtttta aagaaattga atatgaagtg atgagagatt
    33421 cggctgataa tgcaatcgtt gtctgcaata tggaaaattt tgacccggtg ggtatccata
    33481 caggtgattc tatcgtattt gcccctagtc aaacattgtc tgaccatgaa tatcaaatgc
    33541 ttcgtgatgc ttctctttcg attattcgag cacttaagat cgagggaggc tgtaatgtcc
    33601 aactcgctct tgatccacat agttttaatt attatgtgat tgaagtcaat ccgcgtgttt
    33661 ctcgttcatc tgcgttagca agtaaggcga ctggctatcc gatcgctaag ctagctgcta
    33721 aaatagcgct tggattaaca ttagacgaaa tgaaaaatcc tgttacagga acgacttttg
    33781 cagaatttga acctgcatta gattatgttg tagctaaaat tcctcgctgg ccgtttgata
    33841 agtttgaaaa cggagaacgg cttttaggca cgcagatgaa agcaactggt gaagtgatgg
    33901 ctatcggtcg gaatatcgaa gagtctttat tgaaagctgt gcgttcttta gaaatcggta
    33961 cacaccatat cgaacttcct gagttggcag acatcacgga cgatgaccta atccaaaaag
    34021 tggttcgagc acaagatgat cgcctgtttt atgtagctga agctattaga agagggtaca
    34081 ccatagaaga attagcggat ttgacaaaaa tcgatttatt ctttttagat aaactgttgc
    34141 atatcgtaga aatcgaacag cagttgaaac agcagccgtt ggatgacaag atattgaaaa
    34201 cagcgaaaca aaacggtttt acagacaaaa aagtggcgga gctgtgggaa ataaacgaag
    34261 aagccgttcg ccagatgaga aaagaaaatg atttgattcc tgtatataag atggttgaca
    34321 cttgtgcagc ggaattcgaa tcaaaaacac cttattttta tagttcctat gaatatgaaa
    34381 atgaaagttc acgatcagaa aagccctcaa ttctagtact gggatctggt cctatccgta
    34441 tcggacaagg ggttgaattt gactatgcga ctgtgcattc tgtcaaagca atccagcagg
    34501 caggatacga agcaatcatc atgaacagca atcctgaaac agtttcgact gacttttcta
    34561 tttcggataa attatatttt gagccattga ccttcgaaga tgtgatgaat gtgatcgagc
    34621 tagaacagcc gataggagta atcgttcaat ttggtggtca gacagcaatc aacttagccg
    34681 aacctttgac tgctgctggt gtaaaaatct tagggacaac gatcgaagac ttggatcgtg
    34741 cggaaaatcg tgatttattc gagcaagcgc tgaaagagtt ggatattcct caacctttag
    34801 gggatactgc tacaagtaaa gaagaagccg tggttatcgc ttcgagaatt ggttatccag
    34861 ttcttgttcg tccaagctat gtattaggcg gacgtgcgat ggaaatcgtt gaaaaccagc
    34921 gagatttaga agattacatg gaacatgctg tgaaagcatc tccagaacat ccagtactag
    34981 tggaccgtta cttgcttggt ggtgaatgcg aagtggatgc gatttgtgac ggtgaaacag
    35041 tactcatccc agggatcatg gagcatattg agcgtgctgg tgtccattca ggggattcaa
    35101 tggctgtgta tccacctcaa acattatctg aatcgatcaa gaaaacgatc gaagattaca
    35161 cgatccgctt agcattaggt ttgaattgtg tcgggatgat gaatatccag tttgtcatcc
    35221 atgaaaatga ggtttatgtg atcgaagtca atcctcgagc aagtcggact gttccattct
    35281 taagtaaggt gacaggaatt ccaatggcac aaattgcgac gaaagctatt ttgggagaga
    35341 aactaaagac acttggttat gaaaacggac tttatccaga aacaaaagaa gttcatgtta
    35401 aagcacctgt cttttctttt acgaaattgc agaaagttga tacttactta gggcctgaaa
    35461 tgaaatcgac tggagaagta atgggatctg acagacaatt agacaaagct ttgtacaagg
    35521 cattcgaagc ttctggatta cacttgcctg aatatggagc cgtattattt acgattgcgg
    35581 acgaaacaaa agaagaagca ctagaattag ctaaacgttt tacagaaatt ggctatagtc
    35641 tgataggaac gaaacataca gctgcttatt tcgaaaaaaa tggtctggtc gttgaaacag
    35701 tagcgaaaat ttctgaggaa gcagcagaaa aaaatgtagt cgacttaata agagatggta
    35761 aaacacaagt ggttgtcaat acgattgata aagatcgggg aaatgcttct aaagacggtt
    35821 tcatcatccg aagagaagca gttgaacatg gtattccatt gtttacttct ttagacacag
    35881 cagatgcgat cgttcgagtg atggaatccc gtgctttttc aactaaccct atttaacggc
    35941 gagcacatgt taagacaaaa agacgaaaaa ccgttgcagg aggggacaga tgaaacaaga
    36001 aatgatggaa atcgttcagc aaaaacagct ggcaccaaag atttttgaaa tgacattgaa
    36061 aggtgatcta gtaaaagaaa tgcagcaacc aggacagttc ttgcatattc tcgtgccgcg
    36121 tgcagacttg ctattacgcc gaccgattag cttgaatcat atcgaccacg aaacaaatac
    36181 ttgccgtatc atttatcggg tggaaggtga ggggactaga gtcttctcta ctctttctgc
    36241 cggagaccat ttagatgtca tgggtccttt aggcaatggt tttgatttgt ccaatcttca
    36301 tacaggagac gaagtattta taatcggtgg aggaatcggt gtccctcctc tatatgagtt
    36361 atcaaaacag ttagtcgaaa aaggaattaa gccaacgcat tttttaggtt ttgccacaca
    36421 tgaagtcgtt tattatgaaa aagaatttaa tcgtctagga gaaacaagaa tcgctactga
    36481 cgatggaaca tacggtatgc acggaaatgt cggtaattta ttgttaggaa cgaagacgga
    36541 acctacagct gtttttgcat gtggttcaaa cgggttattg aagacagtgg aacagctgtt
    36601 ttcttcgcat caaaatgtcc agttatcatt agaatcccgc atggcatgtg ggataggggc
    36661 ttgctatgct tgcgtttgtc atacgccgga agatgaaaca ggaacaaaaa gtgtgaaagt
    36721 ttgtgacgaa ggaccgattt ttaaaatagg agaggtaatt atatgaatga gaatccttta
    36781 gcggtcaaac ttcctggcct agaattaaaa aatcctatca tgccggcaag cggctgtttc
    36841 ggttttggca aagaatacgg aaaatactat gatttgaatc agcttggatc gatcatggtc
    36901 aaggcaacga ctccaaatgc ccgctttggc aatcccacgc caagagtggc ggaaacacca
    36961 tctggcatgt tgaatgcaat tggtttgcaa aatcctggtc tagacgttgt tatgcataaa
    37021 cttcttccag aattagagga gtacaatgag ttacccatca tcgcaaatgt tgctggtgct
    37081 tgtgaagaag actatgtaga agtttgtagc aaaattggcg aagcaccaaa tgtgaaagca
    37141 atcgagctga acatttcttg cccaaatgtc aaacatggcg gaattgcttt tggaacagat
    37201 tctgatgttg cttttcaatt aacacaagca gtaaagaaag tttcttctgt tcctgtctat
    37261 gtcaaattgt ccccaaatgt aacagacatc gttccaatcg ctcaggcaat cgaagctgga
    37321 ggggcagatg ggtttacgat gatcaatact cttcttggta tgcggattga ccttaagaca
    37381 agaaaaccga ttttagccaa tcaaacaggt ggactctccg gaccagcaat caagcctgtc
    37441 gctattcgtt tgattcacca agtagcaagt atatccagcc ttccaatcat tgggatgggc
    37501 ggcgtgcaga cagtggatga tgttcttgag atgtttatgg ctggagcaag tgctgtagct
    37561 atagggaccg cgaatttcac cgatccgtat atctgtccta aactaattaa agagctccct
    37621 gtgcgaatga gcgaactagg catcgaatcg ctggaaagat taatcaaaga agtgagagag
    37681 gaacgtgaac gatgaatcaa ctagaccaac gaccaattat tgcattagat ttttcaacta
    37741 ggcaagaggt agaagacttt cttcgatttt tcccgaaaga agaaaaattg tttgtgaaaa
    37801 tcggaatgga attgttctac caagaaggac ctgaaatcgt acgttatcta aaagaggctg
    37861 gtcataatgt atttttagat ctgaaacttc acgatattcc gaatacggtc gaaaaagcaa
    37921 tgcggggatt ggcaaagctt ggtgttgatc tcacctgtgt tcatgccgct ggcggtattc
    37981 gtatgatgga ggcggcaatg cgaggattag aagaggggac tccagaaggt ggaaaacgtc
    38041 cgctacttct agctatcact cagcttactt ctacaagtga agaagaaatg catgccgatc
    38101 aactaatcga agtttcttta gaaaaaagtg tgatccatta tgctagctgt gccaaaaaag
    38161 caggattaga tggtgtggtt tcttctgctt gggaagtaga agcgatcaaa gaaatggcag
    38221 gagacgaatt tgtttgctta actccgggta taagaccaga aggaacagtc gcaggagatc
    38281 agacaagagt agtcacacct tctcaggcaa aaaagatcgg ctcaactttt atcgttatag
    38341 gtcgaccaat cactcagtca actaatcctt ataaagcata tcaaacgatt caaacagaat
    38401 ggagctaacc taaaatgaat gttgaacaaa gtatcgcaaa agacttatta gaaattgaag
    38461 cagtattttt aaatccaagt aatcctttta catgggcaag tggaatcaaa agcccaattt
    38521 actgtgataa ccgaatcacg atgagctatc caaaagtccg aaaagagatc gcaaaaggat
    38581 tagcaagcaa aatcaaagaa gcttaccctg aagtacaagt gattgctgga actgcaacag
    38641 caggaatccc tcatgccgca tgggtagcgg aaatactgga tttgccaatg gtctatattc
    38701 gaagtaaggc gaaagatcat gggaaaggta accaaatcga aggacgtata tttgaaggtc
    38761 agaagatggt tgtgatcgag gatttgattt ctacaggcgg aagtgtcttg gaagcagctg
    38821 aagcagcaaa aagagaaggt gcggatatcc ttggtgttgc ggctatcttt acgtatgagt
    38881 tgccaaaagg aaaagcaaat ttcgaaaaag ccgaaatccc tcttatgacg ttgaccaatt
    38941 attctgtatt gattgaagca gctttagaag atcgttatat cgatgagcaa gaattaactc
    39001 tgctaaaaga atggaaaaaa gatcctgaaa attggcagat ttaagcgcgc ttgatgaaga
    39061 tagagaaatc cgatttgtgt ctccagaatc atcaattgga atagcaaaaa tccaaaaact
    39121 gattgcgaga agcatctttt catcaggtat gaaaagaaac ttctatcgtg atcagttttt
    39181 gattttttta aaattcattt ttctaagact ggttagcaga aagaagagaa taat
//
LOCUS       DAMYXM010000020        37221 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.021, whole genome shotgun sequence.
ACCESSION   DAMYXM010000020 DAMYXM010000000
VERSION     DAMYXM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.021"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            149..1114
                     /locus_tag="PNS21_001197"
     CDS             149..1114
                     /locus_tag="PNS21_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDI5158915.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(1171..1401)
                     /locus_tag="PNS21_001198"
     CDS             complement(1171..1401)
                     /locus_tag="PNS21_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HDI5158916.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1487..2374)
                     /locus_tag="PNS21_001199"
     CDS             complement(1487..2374)
                     /locus_tag="PNS21_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDI5158917.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTNTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(2463..3686)
                     /locus_tag="PNS21_001200"
     CDS             complement(2463..3686)
                     /locus_tag="PNS21_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDI5158918.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(3703..4749)
                     /locus_tag="PNS21_001201"
     CDS             complement(3703..4749)
                     /locus_tag="PNS21_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDI5158919.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(4913..6640)
                     /gene="ptsP"
                     /locus_tag="PNS21_001202"
     CDS             complement(4913..6640)
                     /gene="ptsP"
                     /locus_tag="PNS21_001202"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDI5158920.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(6640..6906)
                     /locus_tag="PNS21_001203"
     CDS             complement(6640..6906)
                     /locus_tag="PNS21_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDI5158921.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            7385..9619
                     /locus_tag="PNS21_001204"
     CDS             7385..9619
                     /locus_tag="PNS21_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI5158922.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            9767..10087
                     /locus_tag="PNS21_001205"
     CDS             9767..10087
                     /locus_tag="PNS21_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDI5158923.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(10292..10515)
                     /locus_tag="PNS21_001206"
                     /pseudo
     CDS             complement(10292..10515)
                     /locus_tag="PNS21_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(10713..12293)
                     /locus_tag="PNS21_001207"
     CDS             complement(10713..12293)
                     /locus_tag="PNS21_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDI5158924.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(12694..14070)
                     /locus_tag="PNS21_001208"
     CDS             complement(12694..14070)
                     /locus_tag="PNS21_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDI5158925.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(14198..15316)
                     /locus_tag="PNS21_001209"
     CDS             complement(14198..15316)
                     /locus_tag="PNS21_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDI5158926.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            15458..15892
                     /locus_tag="PNS21_001210"
     CDS             15458..15892
                     /locus_tag="PNS21_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5158927.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(15970..16503)
                     /locus_tag="PNS21_001211"
     CDS             complement(15970..16503)
                     /locus_tag="PNS21_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDI5158928.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(16597..17775)
                     /gene="mutY"
                     /locus_tag="PNS21_001212"
     CDS             complement(16597..17775)
                     /gene="mutY"
                     /locus_tag="PNS21_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDI5158929.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(17768..18565)
                     /gene="recX"
                     /locus_tag="PNS21_001213"
     CDS             complement(17768..18565)
                     /gene="recX"
                     /locus_tag="PNS21_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDI5158930.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            18672..20054
                     /gene="rlmD"
                     /locus_tag="PNS21_001214"
     CDS             18672..20054
                     /gene="rlmD"
                     /locus_tag="PNS21_001214"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDI5158931.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(20228..20806)
                     /locus_tag="PNS21_001215"
     CDS             complement(20228..20806)
                     /locus_tag="PNS21_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HDI5158932.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            21128..21598
                     /locus_tag="PNS21_001216"
     CDS             21128..21598
                     /locus_tag="PNS21_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDI5158933.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(21706..22932)
                     /locus_tag="PNS21_001217"
     CDS             complement(21706..22932)
                     /locus_tag="PNS21_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDI5158934.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(23686..23886)
                     /locus_tag="PNS21_001218"
     CDS             complement(23686..23886)
                     /locus_tag="PNS21_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDI5158935.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(24020..24628)
                     /locus_tag="PNS21_001219"
     CDS             complement(24020..24628)
                     /locus_tag="PNS21_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDI5158936.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(24713..25183)
                     /locus_tag="PNS21_001220"
     CDS             complement(24713..25183)
                     /locus_tag="PNS21_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDI5158937.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(25231..26145)
                     /locus_tag="PNS21_001221"
     CDS             complement(25231..26145)
                     /locus_tag="PNS21_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDI5158938.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            26291..27094
                     /locus_tag="PNS21_001222"
     CDS             26291..27094
                     /locus_tag="PNS21_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HDI5158939.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            27272..28219
                     /locus_tag="PNS21_001223"
     CDS             27272..28219
                     /locus_tag="PNS21_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDI5158940.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(28287..28580)
                     /locus_tag="PNS21_001224"
     CDS             complement(28287..28580)
                     /locus_tag="PNS21_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HDI5158941.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(28608..29273)
                     /locus_tag="PNS21_001225"
     CDS             complement(28608..29273)
                     /locus_tag="PNS21_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDI5158942.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(29413..30045)
                     /locus_tag="PNS21_001226"
     CDS             complement(29413..30045)
                     /locus_tag="PNS21_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI5158943.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(30052..31119)
                     /locus_tag="PNS21_001227"
     CDS             complement(30052..31119)
                     /locus_tag="PNS21_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDI5158944.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(31116..31847)
                     /locus_tag="PNS21_001228"
     CDS             complement(31116..31847)
                     /locus_tag="PNS21_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDI5158945.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(32016..32495)
                     /gene="greA"
                     /locus_tag="PNS21_001229"
     CDS             complement(32016..32495)
                     /gene="greA"
                     /locus_tag="PNS21_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDI5158946.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(32631..33806)
                     /gene="mltG"
                     /locus_tag="PNS21_001230"
     CDS             complement(32631..33806)
                     /gene="mltG"
                     /locus_tag="PNS21_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDI5158947.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            34010..35179
                     /locus_tag="PNS21_001231"
     CDS             34010..35179
                     /locus_tag="PNS21_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDI5158948.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(35446..37080)
                     /locus_tag="PNS21_001232"
     CDS             complement(35446..37080)
                     /locus_tag="PNS21_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158949.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPN
                     KDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTI
                     RYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT        11372 a         7377 c         6085 g        12387 t
ORIGIN      
        1 taatttcttt tattagaaaa atcatctatc tcacatattt tgattatcaa agtattttct
       61 gtgaacagaa ctatagaatt ccctaaaact tgtccattat ttcaaaattt gttatagtct
      121 ttttattcaa agcgtaaaag gagatcctat gttaaaagaa aaaatgaaaa ctctccccat
      181 gctgccgggt gtctatctta tgaaagaccg agatggtaag gtcatctatg ttggtaaagc
      241 aaaacggctc aaaaatagag taagcagcta ttttcatcag aataaacagc attcaaaaaa
      301 agtattacgt atgattcatc atataacaga ttttgatttc gtcgtagtag acacggaatt
      361 agatgcttta ttgctcgagt gtcaattgat ccaacattac cgtccttttt ataaccgaca
      421 aatgaattat ttcagtaatt ataattatgt acatattacc aataagggat ttgtattaac
      481 agatactcct acggctcgaa catacgggcc ttttcgtctc tataaaaaaa tgccgtctat
      541 tttgcgtata atggaagaaa cttatcaaat gccttggttg tctgaaatta gtttgttagc
      601 tttgcgagtc cagcttcctg acctacagga aatgactttt gaacagaaaa aaaaggaact
      661 ccaagggctg tttcaaggcc gaaataaaaa gctactgact tacttgaaaa aaagacaaca
      721 acatttcatc tatcagttga atttcgaaaa agccggtatg cttcaaaaag atatcgaatt
      781 agttacttat tttattagac ggatccagga acaaaagcag tttttaagaa caccatcgct
      841 caccttcagc atgccattag cagcagatga gagtcagaaa aagcactacc tcgtttgtta
      901 tggtcaactt gcagaaacaa tgattacctc tggtgacgtt tctcctgact tctattatga
      961 aaagaaagaa gctcatttat ctttgaaacg tcaattgtca aaagaagaaa tcgatcctgt
     1021 acaaatcttg atcagttacc gcaaaaaatt agaaaaagaa cagtttgaaa ttgaactgtt
     1081 aaacaaaaaa gaagctgaaa agcaactaaa ttagttgctt ctcggcttct tttaggcatc
     1141 ttcaacttca aatgtcaatt atcctaaaat ttattggatt aagtcataga tttccatcgc
     1201 tacgatatcg atattatcaa attcgtattg ctgatgagta tctgtttcag ttaattcaaa
     1261 tgtatcggtc gctttatcat aagttactgt acacttctct acgccttctt tttcaaaacg
     1321 gcgaacttgc agttcattat ctgctgactc gaccatagcc tccagtcgtt ttatgattgc
     1381 gactaattgt gaatgtgtca tcggaggtcc tccttttctt atttctgtta tattgtatca
     1441 aaagcatagc ctacttgcat tgattttctc taaaaaaact cgttttttat gatttttttt
     1501 ctggtatttt tccttcaggc caccaaagtt tgatcaaagc ttgggttccg tcattctcaa
     1561 aacctttatc tgccaactct tcatatagtt tcaatgcttg ggtagttgct ggcaatacaa
     1621 gatccatttt ttctgcttct tctaatgcaa tctttaaatc tttgataaaa tgtttgacaa
     1681 aaaaaccagg tgtgtaatct tcttttaaaa tacgaggaga ataattgctt aaagaccagt
     1741 tagctgcact tccacctgaa agcgtctcaa tcactttctt taaatcaagt ccgttttttt
     1801 gtgcatagac aagcatttcc gtcattcctg tcatcgtacc tgcaatcatg atctgattag
     1861 ccatttttgt atgctgacct ttcccagcag aaccatgcaa tgtaaatgtc gtaccaaatt
     1921 ctttgaataa aggcagtact tgatcataaa cggcttcttc tccaccgacc atgatcgtca
     1981 gtgtaccatt ctttgctcct aagtcaccac cggataccgg tgcatctaac gcatctgcgc
     2041 ctttttcttt tgctgttttt gcaatttttt ctgccaggct tggtgtattt gtagtcaaat
     2101 caactaaaat atgtcctgag atatctgcag aaaaaatccc cgactgtcca tagtagattt
     2161 cttctacatc tttggggtaa cctaccattg taaagagaat attactttgt tctgctaccg
     2221 cttttggtgt atctgcccag atagctcctt ctgttactaa aggatcagct tttgcttttg
     2281 tccgattata gacagctact tcgtgtcctg cttctagtag atggcgtgcg acagcacttc
     2341 ccatgacgcc tgttccgata aaaccaagtt tcattttttc gacctcctaa aaatcaaaaa
     2401 tccttgcttc tatgctagta tacgctagaa acaaggattt tttcataaaa aatgtttgaa
     2461 ttttatcttt taaatgaatt taattttatt ttgattcgtt caatcgagtc ttcttcttct
     2521 tttcttcgca tctcttcttc atgataaaca accatgtctt gataaaattc gatcatagct
     2581 tcgccgaaat gtgtagatga aatatcataa agtttagctt ctaaaacttt tggatcttta
     2641 ggaacttcac tggaaatata atcaataagt gctggtgcaa agtcttcgtc ggtttcaaaa
     2701 gttttcccta atgaaggatg atcgaacaat cggttcaaat aagcatttcc ttccacgaca
     2761 caaggtgttc cggctgccat cgcttctgta taagtaagcc cttgtgtttc tgaagtagaa
     2821 gcactgacaa aataatctgc cgctttataa taaagtgcta catcattgtt gtccacttct
     2881 cctgtgaact gcacaaattc ttctaactgc agttctgaga tcaattcctc taactcttct
     2941 acataaggtc cttttcctac gataactaat ctggcgttag gataagcgga gatgacctga
     3001 ggaaacccat taatcaaggc ttggatattt ttctcataag aaatcctgct taaagaaaga
     3061 atcatcaaat ggttttcctg aagacctagt gactccctta atttgctgat atcctgctga
     3121 gtgatatcag aacgtttgaa tttttcaatg tcgataccag taggaatcac gcgaagcggt
     3181 gctattacac catagctgcg taatgtatca ataacacgtt cacttggaca aactacacct
     3241 gtcgtatgat ttgcaaataa tctagacaag tattttacat gggattgcct gatcactttt
     3301 cctttaccga tataatgaag gtagtcttca tacatcgtat ggtaagtatg gatcagaggt
     3361 attttcaact tcttagctac catcttccca agtatccctg ctccgaattc tgtatgggta
     3421 tgaataagat ctagttctag ttctttggca accaaataag cataccacat gcctctaaca
     3481 acaactcttc gatccttgaa agaaataaat ggtacacttg gcattcgaat cacatctttt
     3541 tccaacttat ccgcattagg atcggtcgtt gtaaaaatat agacttcatg cccatgtctt
     3601 tctaattctt gttttaatgt tttgatcgat gtagcaactc cgctgacttg cggaaagtag
     3661 gtatcggtaa aaaagccgat tttcatgttt ttcgcctcca aactagtcct tgacctgatt
     3721 tacttttttt aatttgctag agactttttc attcagtaca ctttcgtata catttttaag
     3781 ttcttcaccg attttattga tacttcgctc ttttgctgtt tgataaccgg cttgtgtcgt
     3841 actcggtagt tcttgattgg taatctgttg gatcagtcgg ataaattctt cgtttgtctt
     3901 ccccatatag cagttcttgc tatcttctaa ccagccatta tacactggaa tatctcgaac
     3961 aagtacgttt tgtttgcttg ctaaagcttc taaaacaacg atcccttctg tctcttcata
     4021 agaagggaaa aagaatagat ctgcacctga atacgcacct tctatgatct ctccacgaat
     4081 gtaaccagga aacaggacat tttcaggatg atcttctttt acaattttcc ggatgttccg
     4141 aggaatcgag tacatgggaa catgaccaaa ccaaataaat gtgtattcgg gcatcttctt
     4201 agcaatttct acgaaatcaa tgattccttt acgttcgaaa aacaaaccga cacaaatgat
     4261 tactttttga tccggagaca gtttgaaata ttctctgaac ttttgttctt tcattggatc
     4321 agggtgatat ttctctaagt cgatcccatt ggaaatagct tgaatcggca cacggatacc
     4381 atagctttcc aacaactgtt ttgaataagg tgttggcgtg atcagatagt ccgctttttg
     4441 gtacaatcct accagatatt tttttacgat aggagaaagc tggttcgatc cgataaagga
     4501 atttcggaaa tcttcctctg tagaatgcgc atgatagaca actttttttc cttctcttct
     4561 ggctttattg accatattat gactattgat tccgtatgta ttgatgtgca ggatgtcata
     4621 atcttctttt tcatctagtg tataagagat accgacttcc gtcagagcac gtttttgatg
     4681 atcaagcgca cgcccgattc cagattttgc aagcatcttt tcactttcaa aatataataa
     4741 taccttcacc ttacgtcccc cttcacttat atagtataac atagcctaca aactgtcact
     4801 caaacttaag ccctattttt gtctattctt actgacaaaa acgttttctt aaaaaagaac
     4861 aaaaaaactg gcgtagctct tttacaagcc acaccagctt ttgatacgtt gtttatttta
     4921 catattcatg aacaagttca acgacttctt ccatcatatc gcattctttc aatgcacgat
     4981 cagcaagttc agccattttc gctgtgtcca agcgtttcat taaactacgc gttttaagaa
     5041 tagatgtcgc actcattgag aactcatcta gacccatacc tactagcaat ggaacagctg
     5101 tttgatctcc agccatttct ccacacatac ctgcccattt tccttcagcg tgtgctgcat
     5161 caattacatt tttgatcaaa cgtaaaattg atgggttata tggttggtac aagtaagaaa
     5221 cgcgttcgtt catacggtct gctgccattg tgtattgaat caaatcgttc gtacctacag
     5281 agaagaaatc tacttctttt gcaaacttgt ctgctaagac tgctgctgct ggaatctcga
     5341 tcatgatgcc gacttggatc gtatcagata cttccttgcc ttcacttatc aatttttgtt
     5401 tttcttcttc aaagatcgct tttgctgcgc ggaattcttt gagtgtagct accataggga
     5461 acatgatacg taaattacca tgaacagatg cacgaagcaa tgcacgcatt tgtgtacgga
     5521 acatttcgtc gccttgttca gataaactga tacgcaatgc acgatatcct aagaatggat
     5581 tcatttcatg aggtaattgc agatatggta gttctttatc tccaccgatg tccattgtac
     5641 gaacaactac aggttttcct tccatacctt ctaatacagc tttgtaagct tcatattggt
     5701 catcttctgt tgggaagtct ggagaatcca tatacaggaa ttcagtacgg tacagaccaa
     5761 ctgcttctcc accattatta tggacaccaa ctagatcttt tggtgttccg atgtttgcag
     5821 ctaattcaaa atgcttgccg tcagctgtga cagtttctgc attttttaat ttttcccatt
     5881 ctgctttttg tgctgcgtaa tctgcacctg ctttttcaaa ttcagctttt tgttcatctg
     5941 taggatcgat gattacatcg ccttcgatcc cgttgacagc caaaatatcg ccttctttga
     6001 ctttagcagt aatttctttt gtcccaacga ttgctggaat ttctaaagaa cgtgccatga
     6061 tagcagaatg tgaagtacgt ccaccaatgt ctgttacaaa cgcttttaca aaattgcgat
     6121 ctaattgtgc tgtatcactt ggagtcaagt catgtgcaac aacgaccact tcttcgttga
     6181 tcattgatgg atttggtaaa gtaacgccta ataagtgcgc taaaatacgt ttagcaacgt
     6241 cacgaatatc tgcagcacgc tcttgcatat aagcattatc ttccattgct tcgaacatac
     6301 cgatatacat atccgttact tcttttagcg ctgattcagc atttacgctg ttgtctttaa
     6361 tattttgctt gatttgacct accatttctg gatctgaaag aaccatcaaa tgggcatcaa
     6421 atacttgcgc ttctgcttca cctaagcttt gcgctgcttt ttcacgaatt tgctgcaatt
     6481 cttcagtaga tttagctaat gcagcatcca aacgagcttc ttcagccgca gtatcttcta
     6541 ctgttgcctt actgaatgtt aaatccggtt gaactagcag gtaagctttt gcaacggcta
     6601 caccgtcact agcggcaatc ccttttagca tttcaaccat tattcagata agccttcttt
     6661 cttcattgtt tcaacgattg ctgccattgc atcagcttcg tcagcacctt ctgcagtaat
     6721 agtaacatca gaaccttgac ctacacctaa agacataacg cccatgattg atttcaagtt
     6781 tactgattta cctttatact ctaagtttac atcagagtta aatttgcttg ctgtttgtac
     6841 taatagtgta gctggacgag cgtggatccc agtttctgct actacgtgaa attctttctt
     6901 ttccatatta atcggtctcc tttaagtgag tgttgttttt atgtgagggt tttctttttt
     6961 agtaattcag agaatcctaa caaatgccac cctttcatct gtaaagatta acattttttt
     7021 tcgttagata caacagtttt cattcaattt cagataaaag tattcaaatt attccggatt
     7081 ctttttcatc cttttcttca cattttataa caaacgtgtt tatttccttt ttcttgtcgc
     7141 tttctcttct cctgacgttt atcattcttt cctttgaccc tctttgtttt catatgaaac
     7201 gaattacttg cttccctttt tatttttagt ataataagag tcaaaggtca ggaatggtca
     7261 atagagattt caccttttct acctcataaa tctcttaaaa aactaggggt tattctttta
     7321 cctcatgaaa aacgagatgt aaaatatgga tgtacccatt tttgtgaaag gacgtgaatg
     7381 attcatgctt tgtcaaaact gcggcaagaa tgaggcaacg attcacttgt atgccactgt
     7441 caatggtcag cggacacaac tcgattactg tcagagctgt tatcaaaaaa tcaaaaatca
     7501 acaaaatgga ggtctgacta atatggccca aaatgatcca tttggatttg gcagtttgga
     7561 cgatcttttc cgttccatgt ctcgccaaat gcaacagcaa ggaaattatg agcaaacacc
     7621 accaacacaa tttggcggaa acaacaactt caatggtggt cagccacctc aaggcaatac
     7681 agaaggttta ttgggagaat acggaatcaa tatcacagaa caagcacgac aaggtgatat
     7741 cgatcctgtg atcggacgcg atgacgaaat caaacgagtc attgaaattt taaatcgccg
     7801 aaccaagaac aatcctgtct tgattggaga accaggtgtt ggtaaaacag cagttgttga
     7861 aggtttagca caaaaaatcg tagatggtga tgtaccgcaa aaattaatgg acaaagaagt
     7921 cattcgttta gatgtcgtat ctttagtcca aggtactggt atacgaggac aatttgaaga
     7981 acgtatgcaa aaattgattg aagagatccg tcaagcagaa aatgtgatcc tattcataga
     8041 tgaagttcac gaaatcgtcg gtgctggttc tgctggtgat ggaaatatgg atgctggtaa
     8101 tattttaaaa ccagctttag ctcgaggaga attgcaaatg gttggtgcta ctaccttaaa
     8161 tgagtaccgc atcatcgaaa aagacgctgc tttagaacgt cgtatgcaac ctgtacgggt
     8221 agatgaacca actgtcgaag aaacgatcag tattttgaaa ggtttgcaaa aacgttatga
     8281 agactatcat catgtaaaat atactgatga ggccatcgaa gcagccgcaa ctttatccaa
     8341 tcgttacgtc caagatcgct tcttgccgga taaagccatc gatttgctag atgaaacagg
     8401 ttctaagaaa aacttgacga tccaaatcgt tgatcctaaa acaatcgaga aaaaactgca
     8461 agaagcagaa gaacaaaaag ttttagcttc tcgagaagaa gatttcgaaa aagcagctta
     8521 ttatcgcgat cagatcaata aacttcaaaa aatgaaagaa cgtcagctca ctgaagagga
     8581 aacacctgtg attaccgaaa aagatatgga aaaaatcgtt gaacaacgga caggtattcc
     8641 agtgggtgaa ttaaaagaaa aagaacagac acaactgaaa aacttagcag atgacttgaa
     8701 agctcatgtc atcggacaag ataacgctgt agatcgagta gcgaaagcga ttcgccgaaa
     8761 ccgtgttgga ttaaacaaac agaatcgtcc aatcggctcc ttcttatttg ttggaccaac
     8821 tggtgtaggt aaaacagagc ttgcgaaaca acttgcctac gaactattcg gttcacaaga
     8881 ttcaatgatt cgtttcgata tgtctgaata tatggaaaaa catagtgtct ctaaattgat
     8941 tggttctcct ccaggttatg tcggatacga agaagctggt caattaacag aaaaagtacg
     9001 gagaaatcct tacagtcttg tcttgttaga tgaagtagaa aaagctcatc cagatgtttt
     9061 gcatatgttc ttgcaaatct tagatgatgg tcgattgaca gatgcacaag gaagaacggt
     9121 cagctttaaa gatacgatca tcattatgac aagtaatgcc ggaactggta aagtagaagc
     9181 aaacgttggt tttggtgcgg cacgcgaagg tgttactcgt tctgtattga accaattaaa
     9241 caactacttt acaccagaat ttctgaaccg ttttgacggg atcatcgaat tcagtgcatt
     9301 atcaaaagaa aacttgatga caatcgtcac actaatgcta gacgatgtca accaaatgct
     9361 ggcagcacag caacttcaca tcgaagttcc tacgaatgtc aaagaaaaat tagttgactt
     9421 aggctatgat ccatctatgg gtgctcgtcc tcttcgacgc acgatccaag aacaaatcga
     9481 agatggtatc gctgaatttt atcttgatca cccttctatc catgagttga aagcaaaact
     9541 ggataaagat ggaaaaatca tcgttacttc taaaccagaa cgtttggcta aagaatcagc
     9601 cgaagaaacg gctgaataaa tccagtttta tagcgtgaga cggtatttct ttgagaaatg
     9661 ccgtctcatt cttttttcag aaaagtcaaa atactgttca ttttataaaa aagaaattat
     9721 aataagagaa acaagcaatt aaaaatacat aaaagaggtg agcgccatga aattggtaaa
     9781 tgtaacaaac agccattccc gtcttgtatt aaaccagctt gaaaacactg atgcccatct
     9841 agtcaaggtc tacacagccg gaaataccac tattgtctac actgaagccc ctgaacataa
     9901 tgaaattctg ctgatcaatg acaaacgtaa aatccagccg aaagaaatcg aagatgctaa
     9961 aaattatttc ttagataaaa tcaaagacgc cgtttaccac gaggatgaaa tcaaagtaat
    10021 cgaattggca ggccttgttg aaatctcgat tccaaaacat gaattaggcg ctacattagc
    10081 cgtgtaaaca aattttgaac ataataatta aagagcgtgg gacaaaagta aagaacactt
    10141 ttgtctcacg ctctaaacac gtataaacgg cgaaagcaga agcaactcct tcggaaataa
    10201 gctgaaattc acgaaaattg cttttcaaat ttttgtgaat tccagcttat ttcttggagt
    10261 taaacgcttc tgtctcagcc tctttttctt tctagctttt tgatttttct cttttctttt
    10321 gtacttttct cttttcattt tcagttctac gcgtgtcttt atttttaact ttttcctctt
    10381 tatggatgat tgatcgtact ttttgtgcaa tttgctcgtt tttgtcccag tcttttacat
    10441 ctaataatcg acataatcgt aacatctctt ctttttctgt ttttttcttt cacgtctttg
    10501 ccattctgtt tccatgtgtc acactctcct ttctttaatc atatgtacgc aaaataattt
    10561 ttatattttt ttgattagac atttctatga ttttaatata gtaaaaagag ccgtcacgga
    10621 aaatccgtca cggctctaaa caaaatatga gaggctgttt ttgtttggct attcttatta
    10681 cttgatgcag ggcagcttgt gtcaagactt ctttacatca aactttttaa ttttacattg
    10741 ggatatttat ccgcaaacca gcgttctgca aattggttct cgaataagaa caacggttgg
    10801 tcgaaacggt cacgagccaa gatattgcga cttgaactca tcttctcatc caaatcttct
    10861 ggatcgatcc agcgagcgat tttgtgtccc attggagaca tgattacttc ggcgttatac
    10921 tcatttaaca tacgatattg gaatacttcg aattgtagct gaccaacagc tccaatgata
    10981 tattcttccg ttagatatgt tttataaagc tgaatcgctc cttcttgaac taattgatag
    11041 attcctttgt ggaaggattt ttgtttcatg acattttttg cagtcacttt cataaacagc
    11101 tctggagtaa atgaagggag ttcttcataa gcaactttta gttttccttc gaacaatgta
    11161 tccccaatct gataattgcc tgtatcatat acaccgatga tatcgcctgc tactgctttt
    11221 tcgacgtttt ctctcgaatc tgccataaat tgtgtgacat tgcttaattt cagtttttta
    11281 tttgtacgtt ccaaccagac atccattcct cgttcgaatg ttccagaaca gattcggaca
    11341 aaagcgattc ggtcacgatg ggctggattc atatttgctt ggatcttgaa tacgaaacct
    11401 gaaaattctt cctcataagg acttaccatc tgatcatctt gtgttttgtg tccataagga
    11461 gctggtgcca attcaacgaa agtctctaaa aaagtctgta caccgaagtt agtcaatgca
    11521 gaaccaaaaa atactggtgt ctgatcgcca cgagcaattt tttcttgatt aaatgcatct
    11581 ccagcctctt tgattagttc tacttcttct aagacttgtt catataagga attttcagtt
    11641 agaggatgat tttcgggaag cagtccatct tcatccaatt ctactaatcg ttcatcctga
    11701 taattttccg gacgataaaa ctctacacga ttatggtaga tatcataaag accttctagc
    11761 cctttaccca taccgattgg ccaattcatc ggataagatt cgatatctaa tagctcttct
    11821 aattcttcta acaattccaa tggttcacga ccatcacgat ctagtttgtt gataaaagta
    11881 aatatcggga tgccacgttt ttttacaact tggaataatt tctttgtctg tgcttcgatc
    11941 ccttttgcac tatcgattac catgaccgca ctatccacgg ccatcaaagt acgataagta
    12001 tcttcagaaa aatcttcatg tcctggtgta tccaagatat ttactcgttt cccttgataa
    12061 tcaaactgca taacagaact agtaacagaa atcccacgtt gtttctcgat ttccatccag
    12121 tctgatttgg caaaattacc tgtttttttg ccttttacgg tcccggcttg acggatagcc
    12181 ccaccaaaca atagtaattg ttctgtaatc gttgttttcc cagcatccgg atgggaaatg
    12241 atcgcaaaag ttctgcgatt gtccacttgt tgctttaatt ttggattatt cataacctaa
    12301 ctccatttct tccattcaat tatttatatt ttctctaggc acagattgtc cgatctctta
    12361 aagaaagaca atttcatata gggctgtgac aaaagccgtg tagcagtctc tttttccgag
    12421 taaacggcgt tctagagaca gcctatcagc aataagccca cccaagccaa aaatacgaaa
    12481 agctgttttt gcttggctgc tcttattact taatgcagga tagctttttc acaacctttg
    12541 tcaactacta caactgaatc agtatagcat ggattcagcg aatgaaaaag ccaccaaacc
    12601 ataagagtca aatatttttt tgtcagctga ctaaaagaaa agacgtgttg cttgtttatt
    12661 caagcaacac gtctttcata accaagtttc caattatctt cttggctcgt catcttcgaa
    12721 ttcttttctg aagtatcttc gttcttcttc aggttccgct tctgtttctt ccggatcatg
    12781 aaaaatgaca cggattttaa ggattcgaga accttccatt tcttcagaaa ccagcgtaat
    12841 attatcgaca tcaaacgata acttttcgcc ttcatccgga atcatgccta atgctgtaat
    12901 caaataacca gccatcgtat ctacatcact catatgcaga tcactgtcaa aagcttcatt
    12961 gaactcatca atcagcatac gtccttggat gatatattcg tgttcatcga ttttttcata
    13021 aagattctca acttcatctg attcatcatc gatttctccg acgatttctt ctaaaaggtc
    13081 ttctagtgtt gctaacccaa cgacgccgcc atattcatct aataaaatag ccatctgatt
    13141 ctgcgtcttt ttcatttcat ataacagatc atcaataaag accgtttcag gaacgaataa
    13201 aggctcctgc atgattttct tgatatctaa attatcaaag ccgaatttat gggcagcttt
    13261 tagtaaattc tttgtatgaa ggattccgac tactttatct ttgtcttcgt tataaacagg
    13321 gattcttgaa tagttttcgg ataaaacttc attcacattt tcttccacag tgtcgttgat
    13381 atcaatcata aatgcatccg ttcgtgggac catgacttct cgggcaactt tcgtatcaag
    13441 agaaaaaacc ccttggagca tttctaattc ttcattattc aatacgcctt cgttttcaag
    13501 catataacgc atttcatctc tggtcatttt tgaatcttca tcatcaaaag tcatcggcgt
    13561 gatacgcgcc aataaacttg tggaagcaga aagcagccag acgaagggct tagctactac
    13621 accaatcaag cgtatcagcc ctgaagtgaa ctttgcgact tcttctgatt tatttaaagc
    13681 aatacgttta gggtacaatt ctccaaaaac aatcgataca taggtcaaaa tagccagcac
    13741 aatgatattt gcaatatttt ttgccgctgt gccgccacct aataccggag ctaaccgttc
    13801 cgataaagta ttagctagtg aagcaccaga caaaatgttg accaaagtga ttcctacttg
    13861 gattgtcgat aggaaattat ttggattctg caatattttt aaaagctttt gagatttcac
    13921 atctccttca tcagcttttt gctcgattcg attcttattg atcgaaacaa cagcaatttc
    13981 ggaagctgct aaaaaagcat tgatcaatgt caaaacgatc aataataaaa tctgcgctaa
    14041 cagcgactga ctctcagggt cagcattcat aatcgcattc tctccttata aaattaattt
    14101 ttagtttacg atagaaaaaa acgtggcact atagccacgt taaaatggta tcacaaagca
    14161 attttctttg caataaaaac aagtgtacta aaactaccta cgaggtcgta gcaattttct
    14221 tcttgttttc attttgcttg tcttctttga cgattttgat tatataggaa atgatcgtct
    14281 tgcagattgc gtaaaaagga acacctaaga agataccaag caatccggca atatttccag
    14341 ctactagaag aacgataatg atcgttaatg gatggatttt cagtgatttc ccaattacat
    14401 taggataaat gatattccca tccaactgtt ggacgattag cacaaccaca caacaaagta
    14461 atgcttttac cggatcattg aagactgtta ctagaaatgc aggtgctagt cctagataag
    14521 gtcctaaata tggaattagg tttgtcaatc ccgcaatgac cccaaataag aaagcataat
    14581 ccacaccaat taccatatat cctaaaaatg tgaaggtacc aacgaataaa cattcgattg
    14641 cttgtccgct aatgtaatta gccaatgttt tattcagttg ccccaataac tccacaattt
    14701 ggtttcttct tttttctggg aaaaactgtt ggatgtttgg cactagtttt tctccgtctt
    14761 tcaacatata gaacaagatg aatggtgctg taatgattac tacggttgcc gaagcgatcg
    14821 ttccaacgat cgaacctaaa ctattggata ctccactaag gaattgctga atgattgtcc
    14881 cgtaagaaat gtctaattgc tcaaaatatt tattgatatc tacttccttg aacaaaggca
    14941 attcggctac ctcttttaac cagctttcta cctgtttgat gaaatcaggc atattagatg
    15001 ccaatgagga aatttgctgt actagacttg ggatgacact aagtaaaatc caaataattg
    15061 ctgcaactaa aaggagcagc acaattgcga cagcccatat tcttttgatt tttgctttca
    15121 tcaacagctt gacaatagga ttcaataaat agtaaagaaa gcctgcaatc agtacaggtg
    15181 caaataacgt ggaaaaaaat gtcccgatcg gttcgaaaat aaagtttatt tctgatgaaa
    15241 tcatgatcaa tgcagctatg atcaataatt caagcgacca aaacattagt ttagactgtc
    15301 taagtttttc aaacaataga tttcctcctt tctttcttac tagtatatat tatattcgtt
    15361 aaaaattctt ttattctttt tttatttttg aaaacttgcc atacttttta tttgttttat
    15421 tttccagtat actatacgaa aggaaggtgg ctagaccatg aaagttgtac aaacaaaaga
    15481 tacaatgagc aatatttact tagatgctgt acggatcagg catcaagttt ttgtagtcga
    15541 acagggtgtg cctttgtctc gagaaataga caaagatgaa gcccattgta tccacttcgt
    15601 cctgtactca gataaaaaag aaccgcaagg aactgtacgt cttttgcctt tagaaaacgg
    15661 aaaaatgaaa ttacagcgga tggcgattct ttctgaatac agacaccaag ggttaggaaa
    15721 aatattgatt gaagaagcag aaaactttgc taagaatcaa ggctataata cgattttatt
    15781 aggggcacag tccacagctg aaacttttta cgaaaaacta ggatatacag cttacggcga
    15841 tccttttgaa gatgctggca tgcctcatat ttatatgaaa aagactttat aaaaatttat
    15901 ttcgaaaaaa gcaaaaaaca gcaagctttt gagaaaaatc tctcactggc ttactgttct
    15961 tttttgtgtt tacttacgcg acaattgctt gtaacggtta taccaaatat caatatagcc
    16021 ttccgagaac gggccatctc cattattgat ccagtcaacc agaatcttca cattttcttt
    16081 taggatgaag tcaatatcgt tcgggtaatt catcattttg ctgtgcatct catattcatc
    16141 gacatccaat agtcgtttct cgccgtcagg aaataccttg atatccaagt catagtcgat
    16201 atatttcaag gcctcatcgt ccagcaaaaa aggtgaagct agattacaat aataggaaac
    16261 ccccttttct ctgatcattg ctattacatt gaaccagtat tttttatgaa aataaacaat
    16321 cgctggttcg cgcgttaccc atcggcgtcc atcagattca gtgactaatg tgtgatcatt
    16381 cacgccgatc atagagtgtt cgcttgtttt taataccatg gtatctcgcc acgttcgatg
    16441 caatttgccg tcgtgcttgt aactttggat cgtcacaaac tctccttctt ttggaactcc
    16501 catagcaacc cttccttaag cagcattttg tgctttgtgt ttgggtgttc attacaccga
    16561 catcataatt attatatcaa taaaaatgag ctttgcctag aaaggattcc tattgtttcg
    16621 atttttctct aactgatcta ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa
    16681 tgcatcaggc ttcaaccact tcgaagtttt gttctcggta aattcttgtt ctgctccctc
    16741 ggttgcccgt ccgtaaaaaa ggagaacatg ccatttcaga tgactaaaaa tatgagtaac
    16801 ttctcctaaa tgtcgtgtct gccaaacaaa gagttcctgc ttttcaaatg ggagattttg
    16861 ctcttcttct gctactaggt catcaaacag atcgatttct ggctttgttt cccattcttt
    16921 tttcaatcgc tcatactcct cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc
    16981 cagcaatttt tggctatctc tttcttcaaa ataatacgct ccagaatgat tttgaagagc
    17041 tgcactgata taataaacgt cttttggctt tgcttttttt gtttttacag gaaaactcgt
    17101 ttggattcct cgtttgttag ctaaacaaaa ggcttggatc ggacaagtct cacactttgg
    17161 agaagttggt gtacagatag ctgaacctag atccatcatt gcttggttga attcacctgg
    17221 atgcttttca tcaatgattt tccgcatcgc ttcatcaaag attttcctgc tagaagcttt
    17281 tgcgatgtct gcttcaatgc aaaacaatct gctgactact cgcatcacgt tgccgtcaac
    17341 tgcaggttct ggaagtccaa atgcgatact tgcaatcgcg cctgtcgtat atggtcctat
    17401 tcccttcaat gagctaattt cttcaggcgt ttgcggcatt tccccatcaa attctgacat
    17461 gatctgttta gcagcagctt ggatgttccg ggctcttgag taataaccaa ggccctccca
    17521 tgcttttaaa agcttttctt ctggagcatt tgctagctct tcaattgtag gaaaccattc
    17581 catgaaacga taaaaataat cgatcactgt gtctactctg gtttgctgaa gcatgatctc
    17641 agagatccag atacgataag gatcacgatt atagcgccaa ggcaggtttc ttttttcttg
    17701 ttcataccat tgaataaact gatcttgaaa ttcttttgtt tctttatctg tccatttttc
    17761 ccattcttta ttcactttcc tcttcctttt ccgtaatgaa tcgctggatc atatccaaat
    17821 caaatccttt ttgatataag ctttgcttta cttttagatt tcttttttta ggttcaaacc
    17881 ttaaatttct tcgccatagt ttatctcctt gtttgactaa ataatcatat tctatttctt
    17941 gatctgcttc ttgcggaaga tcagctatcg cttgttggat cacttcttga gaaaagcctt
    18001 tcgtcatcaa tgtttgttgg atttttctca ataattcttt ttggcttttt ccgtgatttt
    18061 ttccaaacgc tttttcggct gtctgatgtg cattttgaac ttggtcttca aatgtatatt
    18121 ggctgagcgc ttgttcaatg atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta
    18181 atttctttgg tcctttgtca cttaagcgca tattcgtgcg cacataactt tccccataga
    18241 ccaaatcatc gacaaggtcc aattctttta agcgggctac gattttattt cgatcttctt
    18301 gaaggatttc cttttctttt aagtatgttc ggatttcttt ttccgatcgc aactgataac
    18361 ttaagtaatt cattgctagg tgaaaaccga aatcagctcc gctactcttc ttgatctctt
    18421 ggatagtttc ttcgtctaat tcccgtcctt ttaataatcg atagcgtact aatacatctt
    18481 cagatacttg taaagcttct ccttcagata aatctacgcg atagaaaggg cctttgcctt
    18541 tactgattct cgtaacggtt aacataaaca tcctcctcga acatatattc tctatcatac
    18601 cacagaaaat ccccgtaatc aggtattata tgttagaata aaccacagaa aaaagaaaaa
    18661 gggagatact tatggcagaa acaattgtca aaactggaca aacggttcct ttgaaaatca
    18721 agcgtttagg cataaatggt gaaggaatcg gttatttcaa acggttgatc atatttgttc
    18781 catatgcact accaaaagaa gaagtactag tgaaaatcac gaaagcaact cctcgctatg
    18841 cagaaggaga attaataaaa gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc
    18901 ctgtttacta tgaatgcggt ggctgtcaat tacaacactt agcttatcaa gctcaattgg
    18961 attttaaaaa ggatctgctt ttgcaagcat tggaaaaatt caaaccagca ggctttcgat
    19021 cttatcaact tttgccgacg atcgggatgg atgagccttg gcattataga aacaaagcac
    19081 aatttcagtt acgaaaaaat aagcagactc aaaaaattga agctggttta tacgaagcaa
    19141 actctcatca gttagtccct ataacagact gcttggttca agaacctcga acacaaaaag
    19201 taatgaatac tgttgttcaa ctattgaata aatttgatgc acccatctat gatgaacgga
    19261 caaacagtgg aatcttccgc acaatcatgg ttagaatcgg tgttaaaact ggggagttac
    19321 aagttgtttt tattactcgc agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga
    19381 tcaatgaacg actgcccgaa gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt
    19441 ctctcgtgat gggggatgaa acgatccact tatggggaaa agaagccatt gaagaacaaa
    19501 tcaatgaagt gactttcgat ctttctcctc gtgccttctt tcaactcaat cctcagcaga
    19561 ccgaattgct ttatggcgaa ggaatcaaag cattagatgt ccagcctaat gaaacgatcg
    19621 tggatgctta ttgcggagtt gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag
    19681 tacggggaat ggataccgtt cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat
    19741 taggatatca caacactcga tatgaagtcg ggacagcaga gcaagttctg cctaaatggt
    19801 tgaatgaagg ttttcagcct gatggtatca tcgtagatcc accacgtacg ggtttggata
    19861 acgcattgat ccaatcatta ttgaaatacc ctccaaaaaa acttgtttat atctcctgta
    19921 atgtttctac acttgccaga gatctggtta atttaagtaa aaagttcaag attgaatatt
    19981 tacagtccgt cgacatgttt cctcagactg cacgttgtga agttgtcgtg aagatgacga
    20041 gactcgctga ctaaatgaaa gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa
    20101 cagccaaaca aaaatagctc ttcatgtttt atgaatactg tacttgtgac cgaagagtta
    20161 acaggaggac actatttatt taaaaaaagg gttgtgacat agtttttgtc acagcccttt
    20221 ttgattatta ttggatattc acgtctatca cttgataaaa tgcatttgtc gtatcgctga
    20281 tattccatac tgctaaaatc acattatatc cttttcgatc ctgcggaagt gtgatcgttt
    20341 ggttgactgt ttttggtgga acagttgctt tgtcgtctat ttttgtgatc aattcaaaat
    20401 cactaaattt caaaggttga ttaggattcc aagttggttt ggtaatgtag taatcccaag
    20461 tacttgtccg atgttgtgct gttaaattcc aagtaatatt taatgttcct ggattaacca
    20521 tgtttttatg ccatctgtta gatgtctgtt catctaaagg ctcaaagccg ctgactcctg
    20581 cactagcaat ttttccatca atgaatgtat ttttggtagc ttcgatactt tgcggttccc
    20641 attgtgctct tccgacattt tgatttaagt ttcctgctgc tgatgttcct aggtatgcac
    20701 ggcttcctgg agaagtaata taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc
    20761 ctaaactaac taacccaata aacaataacg tactaatctt tttcatgtag aaacctcctt
    20821 catttgataa ccacattgta aacgctttat tgtattaaca caaacattat gcttttataa
    20881 ttttatgttg aagtaaaaaa ggtgaaaaaa gtaagatgca gattataaca gaagtagacg
    20941 agaaaagtac aaatagaaaa catgagaatc aaatcatatg atctttttca aaaacatcat
    21001 tcatataaac aaatgttcaa ctttttaata acaacaatta tcaatttttt tcttacttca
    21061 caaactaaat acaggtcttg ctgtgagcct tcatggtaaa ctaatattat agaaaggagt
    21121 tagtaaaatg aagatgataa aagatattcg tttttgggtt tgtgttatag gaattgtcat
    21181 attaggcttt ttatcagggt tgcttagcgg aaatcccgga aaatattact attctttgca
    21241 acttccacca tttgcaccac ctagctggat atttgggccg atgtggacct tactgtatat
    21301 attgatgggt atttctcttt atttgttact taatcacaac aacaaaaaac agcgaaacaa
    21361 tcttgtcggt ctttttgtga ttcagttcat ttttaatttt atatggtctg ctctattttt
    21421 taatttaaga aatattttca ttgcagcaat cgatattaca cttttagtta ttttcttgtc
    21481 agtgcttatg tatcagttgt ggctgcatca tcgcctgaca atgtggctca tgattcctta
    21541 ttatttatgg gtactattcg caactctatt gaattattca atttattttt taaactaagc
    21601 gctaaaaaga gacgtcctgt aatttaacac atagaaaaag tgccacctaa gttagatttt
    21661 ttgtctaatt taggtggcac tctttattaa ccaaactatt tttttctagt acccacttgt
    21721 tgttccatac tcattattct ggtcataacc ataaccattt tgttcctgtc cagcgtaagg
    21781 atcataactt tgagtatctt gacttggtgt atagtactga cttgtatctt ctgtttgctg
    21841 ataataggta ttcggatctt ggtactgatc atatgtgttt tctgaatagg aagaataatc
    21901 ggtgtaccct tcactattcg atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc
    21961 aaagaacatc attgaagcat tctcattttt caaattaggt aatgtatcgc ttgtttttat
    22021 atttaattgt ttcttcagtt cattttggat cgaaagaaga tcattttttc ctaaaatttg
    22081 atagtagata tcattgatca tttgtccttc gccttgcaac tggtcttgtt tgattgtttc
    22141 aaacgccggg gtgtagttcg tcgctacatc aagcatgtca tcccaatcta agtccgtcgt
    22201 cacattgttc tcaactgctt ttaaaatttt tctataattg cttacgccat ctaaacttaa
    22261 aactttattg acgattttcg tcactacttc ccgctgacga cgttgtcgac cgatatcgcc
    22321 ttctggatct tcgtgtcgca ttcgagaata ggctaaacct ttttctccat tcaatgtttg
    22381 ttcacctact ggtacatgga caccatccaa agtaaagtca attttattat tcactttgat
    22441 gccaccgacc gcattgatca aatcttccat gccatccaaa ttgatcgtta cataatgatc
    22501 aataggaata tcgagcagtt gctcaattga atccatggcc atttcgacac caccaaatgc
    22561 gtaggcatga tttaatttat ctaccgttcc ataacctacg atattcgtat aaatatctcg
    22621 gtccaaactg atgatcgttg attttttttg tttcggatta actgttacta ccatcatcgt
    22681 atcagaacgt ccttgttccg ttcttcctaa tccaccggta tctaaaccta atagcaaaac
    22741 cgaaaaaggt tctttgtcat caatgcttac tctctcagaa cgtttggagg atattcgatt
    22801 agagttttta gaaattttgt ttactgtttg gctcgcttct cccataaatc tgattccgta
    22861 tgctgataat ccggcaatca gcaaaacgaa tacccctaac aaggttaata gaactttttg
    22921 ccatcgcttc ataatacacc tctgtttcac attttctaaa atattgtctc atataactgt
    22981 tcaatcagca aattcacttt ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg
    23041 attattattt tttaaaattt ctcaaaattc ttaactatca taccataccg tgaaaaagat
    23101 cgaaagaccg aatacacgca gttcgcaatc gttttatctt tttttatgta ttttttccag
    23161 aaaaagcgaa caacaaggta ttttccaaat aatcgtgcag taaaatgatc gcacatacct
    23221 ttcccatctt tttaacagtc tttgcctacc gttcagatta tcattcgatt tcacgacaat
    23281 ttcttcctat gactggtcct tttgttaaac ccatcttcta ttcgttttat ttccagctca
    23341 ctgcatggct cttaaaatga ctcgaactag tgattgcatt accatcatct tttaatggat
    23401 agatatgctc ctcttcaaag taaaacgatt ttcttttaaa gtaatagaat tgacatggta
    23461 atgagtatgt atactagtgg tttcacaaat ggtcttttat tcaacatcca aaaactgaaa
    23521 tttctttaaa atccattact aacgaagaaa atgttccttg cttgataatt agttaggaca
    23581 tgtcaagtga acaaatgcat ttacttagcc aaaaaatcta aaaacttcat aaatagacaa
    23641 aaagacacga ctctctaaga agtcgtgtct ttattcaaca aattattata atttagtcac
    23701 ttcagcagct tgaggtccgc gtgcaccttc aacaatcgtg aattctactt cttgaccttc
    23761 ttctaggctc ttgaagccgt ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc
    23821 gccgtctact gtaataaacc caaatccttt ttcattgttg aaccatttaa ccgttccgtg
    23881 ttccataaat gtatatcctc caaaaaagtt cttgcaagta accttgcaag aacattatac
    23941 ccaatttaga aaacgctagc aagctttcgc tcttttattt tccaaattat ttttaagact
    24001 gatatgacgg aggaaatggt tattttgctg cttcgaaacg tttgtttact tcatcccaat
    24061 taataacatt ccagaatgct gcaatataat ctggtcggac atttttatat ttcaaataat
    24121 aagcatgttc ccaaacgtcc aaacctagaa ttggtgtctt tccttccatc aatggagaat
    24181 cttgatttgc agtagaggta atagctaatt ttccattttc cattacaagc caagcccatc
    24241 cagaaccaaa tcgtccagcg gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac
    24301 caaaagcttc attaatcgct tcttttattt ctcctgtagg ttcgccacca gcatttggtg
    24361 ccatgatttc ccagaaaaat gaatggttag catgtccgcc accattgtta cgtacagctg
    24421 tcttgatatc tgttggaata gcgtccatat cagataataa ttcttctatt gttttttcgc
    24481 ctaattctgg gtatttctca attgctgaat ttaaattcgt cacataagta ttgtgatgct
    24541 tatcatgatg cagatgcatc gtttcttcgt caatataagg ttccaatgcg tcatatgcat
    24601 aaggtagatc tggtaatgtg taagtcataa aaaaattcct cctcaatgtt ttcttaataa
    24661 tgtctacatt acaaagagta acaaagagaa ggaaattctt caattgttat gcctagtcgt
    24721 ctttttcact ttgacctact tcttcttcaa aaacctcagt ttctttaaaa ataaacaact
    24781 tgctgaaaat atagttcgca aggataacaa taatttgagt gatgattttc gccacatact
    24841 cattgctgtt cataacattg atcaacaata ccattgcccc catgtccaat ccgtaagaaa
    24901 ggatacggta gaagaagaac ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt
    24961 ttgaatggaa tacccaaagt ttgttcgtta caaaagcaaa caatacagaa caaaaccacg
    25021 aaatagaatt ggataaggcc atacttaaat caaaatattg gcgtgcgaga gcaaaactaa
    25081 caaaatttac aatcgtagcc aaaccaccaa aaaagagata gactagtatc tcccaatatc
    25141 cttttgcttc taaatatcct tttaactgtc gatactgctt catgagccaa cctaccttat
    25201 taaaaattct tatttatcat actctatatt tcaataactt tcatctttat ttaaaaaaat
    25261 taattttttt tgatcctttg cctttgtctc tgcttggatc gtcacatgtg taataccata
    25321 ttttaatttt aataaagaaa ttatacgagg atatatcttt tcaatctcgc ttagtagctg
    25381 atcttcaaaa tcaatatgtc cgtcgaaaat aattatcttt tcgctatact gccaaatatg
    25441 gacatgatga acatccttta ccccatcgat tgcttcaatt tcttgttcca ttttttcata
    25501 gtcgatagca ggtgcacttt gtatcaaaat agcggcagct tttttgatca ccattatcgc
    25561 ttctatcaat atatatatcg aaatgatcag tgtaataact ggatcgatcc agtagatatc
    25621 gaaaaaacgg atcaaaattg ctccgataat cacacttacc gaagacagtg tatcacttaa
    25681 caaatgcaaa taactagatt ttatattcaa actttcatct gcttcttgtc gtaataataa
    25741 tactgagaac aaattcgcga gcaatccaat aactgctact gttagcatca aatgactatt
    25801 tatcttttct ggtgaagtga accgcttgaa tgcttcaacg gttaaaaaca aggataatat
    25861 gatcaatatg aatgagttga tgaaagcact caatatttct aaacgctggt agccatacgt
    25921 acgtttttta tttgcttctt ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc
    25981 caatgtatca ctaaggttat ggaaagcatc tgaaacaaga gacaaactcc ctgacataat
    26041 ccctccaata aattctgata aagtgatcac cgcattcaaa accactactc caaatagttt
    26101 ttttgatgat ctctgtggct gtgtaggcac tattccttcc tccattttat ttatattgga
    26161 tagtctttag acaagaataa catttttttg atatttcacc aatcattcag ctgtttcctt
    26221 aaagcacttg tatttcactt gatacttcgc tagaattgca agtgatgaca ctactagatg
    26281 aggagtactt atgacaacaa aacaattaat tatcagttcc tttatacgat tcaaagacct
    26341 aaaggaggcg agaaatgtcc cattttggaa agccattgtc tatcttttag ttttgagtat
    26401 aattatggct ttgccaatct cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga
    26461 tgggcaaaag attgctactc gcattcctga ttttacaatc caaaacgggc agcttcaaac
    26521 cgaagaaaaa gaaggattca tttaccaaac aaactccatt atcttcacat ttgatccaga
    26581 gggaaaacga acagaacagg acatctctac tgatttgatg gggaattttg ttagtgtcgg
    26641 gatgttaaaa aacaaactta tcatagctct tcctaataca ggcacgactt ctgctttgtt
    26701 gaacaataac caattagaac ttccctacac caatgaatct ttaaaaaact taacaggtaa
    26761 gcagctccgt tcttttttaa gtgaagcatc tataccactt tggataaaag tcctgacatt
    26821 tatattttct atttatccta gttttctgaa tctgctgatc actttgctat ttaccaacat
    26881 tgctgctttt ctctatgcac gctttcgact aacaaaagct acttttcttg attgtttaaa
    26941 aacattgatc tattcaattt cattgcctac tataattgca acaatcttga tggtctttct
    27001 tccttctttt gacactagcg ccttcattgc ttttgcagga atctttattt ttgctcaagc
    27061 tgtcaaagga tgggcaaaaa tatcgatcaa ttaagaaata aagacaactc gttatttcta
    27121 cttcaataga cccttttaga ggttgtgaca tttttttgtc acagcccctt ttttatttga
    27181 aaaatatttc tttctcaaaa aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac
    27241 acttagagta aagaaaggcg ggaaattaac tatgtcagaa agaatcattt atgcagatcg
    27301 tccatttcgt gatgaattta tcgaagaaat ccatactata gcgcctggtt atatctttaa
    27361 aacagaatta caaccagagg atctacaaaa tgtagagatc agtttgggct ggaactcaga
    27421 ctaccaagaa aaactattag cctcttctac cttgaaatgg gtccaatcta tctcagctgg
    27481 tgttgatcag ctaccccttg agactttttc aaagaaacat attttacttt caaatggtag
    27541 tggcatccac agtgaatcga ttacggagca tgtcttagga atcatccttg ggtattctcg
    27601 tggtcttttt caagcacaaa aggcacagag tgacatgaaa tggttaggtt catcggtcca
    27661 ttaccaatct atccaaggga aaaaaatact aatcattgga actggccata ttggaaaaat
    27721 gattgcaaaa aaatgcaaag cattagacat gaactgctat ggaatcaata catccggtca
    27781 tccagcagaa ggaatggaaa agactttttc tttaaatcaa ctaaaggaag ttcttcctga
    27841 agtagatttc gtagtcaata tcttacctct aacggaggaa acaactgggc tctttgatca
    27901 gcatatgttt gaacactttg atccaaaatc tgtattcatc aatgttggcc gtggtgcttc
    27961 tgttaagaca caagatctgg tgcaggcttt aaataaccag caactttcct ttgcagcatt
    28021 agacgttttt gaggaagagc ctcttccaca agatcatcct ctttggaaaa tggacaatgt
    28081 actgattact tcccacattg caggtttgac acctgatttt caaaagaaat taatggctat
    28141 ttttttaacc aatctgaaaa gctactttgc cacccatgag cttcaaacca atcaagtaaa
    28201 attgtctgca ggatattaat agctgaaaac aatattttca aaagaaaaaa gagaaggtga
    28261 caaaaattgt gtcaccctct ctttttttat tgttttttaa agtgataggt tggctcctcc
    28321 aggttgggat cataatcaac aagtaaatca tatcctttaa agaaccattc atctgcttcc
    28381 tcgataaaaa aaagtgtccc atccatttcc actttcttta caggatgttc tggctgttca
    28441 actgacattc caatcgaaaa accatcatga acctctgttt tgccgtagac ttttccaaaa
    28501 aaacgtattc cgtaagtttc tggcagatca atttctgagt caaaccactt ttgtgctttt
    28561 ggcgttaccg ttaatttcat cccaacaacc tactttctct tttctttcta ttgattcatc
    28621 ttatcgtcta gaatatccac atcgcctgtc tctccaataa ccagtagatt atcattttca
    28681 cgaacatatt catctgctgc aggagaagcg ataaccgttt gatctactct tcgtatggcg
    28741 actactgtta agtcaaaacg ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat
    28801 ttaggattgg agactcggat ttctgctaaa gaatatttat ctgacaactc taggtaatct
    28861 aaaatattct ttgacactaa attatgcgca atccttattc ccatgtctcg ctcgggatgg
    28921 acgacgcgat cagctccgat cttttcaagt actctggcat gatactcatt ttgggctttt
    28981 gctaaaatat ttggtacgcc catttccttt accatcagag taactaagat actggcttga
    29041 atatcttctc caatagcgac aattacatga tcgaaatttc ttatgcctaa tgatcgaagt
    29101 gtcatttcat cctgtgcatt tgccacaaca gcatgagtgg ctatattcat atattcattg
    29161 acccgatctt cattactatc aatagccagt acttcttgac cagcttcgat cagtgttcgg
    29221 cagatacttc cgccaaatcg tccaagacct acaattgcaa aattttgttt caccgttttc
    29281 ctcctttagt atcttctttt ctcagtataa caaagccttt acaaaaacaa aagcaagcat
    29341 cattattgta aaaaagccaa gaaaaataat agttattttc tcagcttttg attatcctct
    29401 ttatcatcgt ttttatttca ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg
    29461 gtcttccact tctagcttag ccaatatatt ggaaacatgt gtttttactg ttttcagtgt
    29521 aataaacaat tcgtccgcta tttcctgatt actttttcct tcagaaatca gcattaaaat
    29581 ttcattttcc cgattcgtta gttcttcatg taatacaggc tcatttcgcc gactcatttt
    29641 ttccatcatt tttgttgtca cttctggttc taatactcgc tcgccacgtt gcgttgccct
    29701 tatagcatca gcaatttcat gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc
    29761 ttcaatggca ggataaactt tttcgtcatc aataaaactg gttacgataa tgattttagc
    29821 ttctggccaa tcttttaaga ttgcttttgt tgattcgata ccatccattt cttccatgac
    29881 taagtccatt aaaatgacgt ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc
    29941 attttcagct tctccaatta cttcaatgtc ttcttgaata gataaataag aagagacgcc
    30001 taagcgcacc atttcatggt catctactaa taaaactttt atcatcctta tctatgcctc
    30061 cttcatcaaa gggaccttga tttctacact tgtaccttga cctttaaaac taatgatttt
    30121 tactgttcca ccaataccag ctactcgttc tttgatattg ttcaatccat aacttcccgt
    30181 tttcgtttca ttcatattaa accctgttcc gtcgtcaata atacgtaaca aaagattatt
    30241 gtctattttg tgcaaatata cctctaattc attggcttta gcatgtctta atgtatttga
    30301 taacaattct tgaacgattc ggaacaagtg atcctcaatg gaactagata gttttacatc
    30361 ttctacatcc catttcagcg aaatctggat tttattttgc aattccttca ataactgctc
    30421 gataccttgc tttaaacttt tctcttctaa attgactgga cgtagatgaa gaagcagcgc
    30481 acgcatctca gactgggaag cgttgatgat ctctgctacc attttcaact gcttgcgaaa
    30541 catttctggt gtctcgcttt tttctgcttg ctccgtcaat gccgacatca tcatcattgc
    30601 tgcaaacaat tgttgcgaaa ctgaatcatg cagctcacgg gctaatcgat gtctttctaa
    30661 ttctaaaatt tcttctttgg tttgtccatc tacatattgg ggacggctcg ttacaatttg
    30721 taattcacta gatacttcaa tcattttttc ttttatctta gtgatttcct tatcaatatc
    30781 tttgatgtat aaatcgtccg atgcacttgg gatcggaaga agcaacaatt tggattcata
    30841 attgccagaa gaaagtgcac gcagcttttc ttcgattttc ccgtattttg tcttttgaat
    30901 aagtgataat aatagaaaga caattaatcc tacacctaag gaaatggcta acaaatggaa
    30961 gataagcgga acatataata aacgtgcacg cagtaattct ccccaccaat gactttgatt
    31021 gctggcataa aaataagtga aaagtgaaaa tagaataata aggaaagccg caatccccga
    31081 ataaacagct agcatcgctc tggatatttt tcccatcata ttcgtatcac ctcgacatct
    31141 ccaatcaagg tattagtaat aatcttcaat ctacgagggt tttcatcata atcactgctg
    31201 tagattttga tttgttcatt tttcaaagaa aattgctctt cttcaaacaa gacatttcct
    31261 actaatgtcg catgctctaa tcggatggct acgcccaaag gtactaagat ccgtgttctg
    31321 cctatccctt ttcttacaat cacaatatta tcatcttttg gcaaaagcgt attgcccaaa
    31381 tctatgatag tatcaccaga aatgacggcg atgttgatat cgtcccattc atagacgtcg
    31441 tttccaatac gttggttacc gaataattgc tgtcttttcc gctcattatt atgtgttttt
    31501 aattgctctg tttggatcat catgatctgt ttctttcgcc aaaaagcatt tttggttaaa
    31561 tctatccctg aaatctctac acctttcaga ccaataaata aaacggcaaa tacagccatc
    31621 atccataaag caggactgtt gactaagcta aaaaaaatga ccaaacttcc taatatcaat
    31681 tgaaaatttt gaaatttagt acgaggatgt ttgcgcatag ctagatatat attgaaaata
    31741 ccgaatatca ctaaaagcaa aagtccagta ttgtttacga tttgccaaac agcaaataac
    31801 agcagtagtg cttcaactac cacaaaaaaa cgccaagaac tattcatcgc tctttctccc
    31861 tcctgacaca ttctcatttc atcataccta tttttttaac tgttggctat aggactttag
    31921 atgtaagatc gtttgttgaa atctaaaata cacaaagaga aaaagatcta gagtgtatca
    31981 tcagtaagat gaactctcta gatctttcag tcagactacg cttgttctac tttgacgatt
    32041 ttaactaaca tatcgccacc tggagtagca atagttactt catcattttt acgtttgcca
    32101 atcaatgctt gcgcaatagg agaatcattg gaaattttgc cagaaaacgg atctgcttca
    32161 gcgcttccga caatcgtgta ctcttcttct tctccatctg gtagttcgat gaatgtaact
    32221 gttttaccaa ttgatacctc atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa
    32281 cgaatcatat tttcaattgt tgtgattctt ccttcaacaa atgcttgttc atctttcgct
    32341 gattcatatt cagagttttc ggaaagatct ccgaaaccgc gggcaatttt gattcgttca
    32401 atgatttctc cgcgtttgac tgtttttagt tcttctaatt ctttttctaa tttttcttta
    32461 ccttctagag tcattggata aactttctct accatgtgta acgtctcctt ttttcgataa
    32521 aataccgcaa aaggtaatct tttgaggaat atagtccaaa aaagattacc acgcttttct
    32581 tcaaatgtaa atgtttttct aaaaatgatc aatgtctaga gcctgttttt ctaagaatta
    32641 tttacatatt tttccactaa ttccatatgt tcttcataag ttttagcgaa ataaacattt
    32701 ccagtgctcg tatctgctac gaaataataa tagtcatttt ctgctggttc taatactgcc
    32761 ttgattgcct cttcgcttgg attgtcaaaa ggtccaggtc catagccggt attggtatac
    32821 aaattatatg gtgaatctac agctgtatct tcatacgtca ctaattcttt gtgttcacct
    32881 aatgcgtata aaacagagat atctgactga agtggcatat ttgctttgat tcggttaaag
    32941 aagacttgtg caatgttttt acgatcattt tcttttacgc cttctttttc aactagagaa
    33001 gctagagtta aaacttcttg tacagtcaaa tttttctgct tgatagctga ataatagttt
    33061 tgcatgaccg tattcgtctt gttcaccatt tcaatcacta gatccttaag cgaagtactc
    33121 gtgtaataat catatgtggc tgggaataag taaccttcca aacggtattt tgtgttctca
    33181 gctttagatg cactttccaa cagttctggg aatttttggt gcaactcgtt aaagaaagtc
    33241 tcatcattca ctaaatctaa aaattctttc ttgtctttac cagttacctt cgctacccgt
    33301 tcagcaatct gatcgatatc gtacccttct ggaatggcaa ttttcccatc tgcaactttt
    33361 gttggctcag aagtaccgcc ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta
    33421 ggcgctaact gataatagcc agcttgaaag ccagttaaat ttttaaattt tgtataataa
    33481 ttgaaaacaa tcccgctttt aataatattg tccttctcca aaatctcacc aatctgcttg
    33541 ttagaagagc cactcgggat ctcgacttgg actaattggt catctgattt atctaaaggc
    33601 ttcaacccgc tatctacata acgatacacg gtaaatccaa gaaatccgcc aataatcaat
    33661 aatgtaagag caatcactaa aatgatcttc ctcacaatct tatcttcttt tttacgcaga
    33721 tttctccgct tgtcgattat atctgctgta gctgccttac tggcttcttt tttatcttta
    33781 cttgggtgtt gtttgtttcc tgacaaaaca actcctcctt actatcaagt tcgtaacttt
    33841 attcattata catgatattt gaattggcgt gaatgatttt atttaaatat tctatcaaaa
    33901 atgtaagtag taaatccatt actataagat taaaataaat gttatcaaga tacaagctat
    33961 tttagcttta ttctttctgt aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg
    34021 aaaacatgct aaaaaaagaa aatattcatt tttaatgatc gttgtcgtgt tgtttagcct
    34081 tagtgcgttc tctacaggaa ctattcttgg atggcaattt tacaataatg aacactctca
    34141 gatagttgcc catgctcaat cagctcaaaa aaagttgata ctgactacca atgaacaaca
    34201 gctcgttgaa aaaatcgatc aactattaag aaaaaatgat tatattggct caatttacgt
    34261 aaagaaaaat aatcgcatca tcatggaaaa aggctatggc tatgcgaata aactcacgca
    34321 acaaccaaat gatccttctc tttattatca gattggttcg gttcaaaaag cattgactac
    34381 tcttttgatt ttaaaacaag tcgagcgtgg gcacctttct ttagatacta agctggctca
    34441 attctatcca caaattgctg gcagccagca aattactatc cgtgacttgc tgtatatgcg
    34501 ctcaggcttg caccgaactg cttctccaac cataccgatg actgatgaag aagtcgttca
    34561 atttgcgatt cagcacttaa aatttacaga ttatcagacc tctcattatg attcattgaa
    34621 ctttaccata ttgactggaa ttttgatcaa attaacaaat caaccgtatg aaaaattatt
    34681 gaccgatgat atcatcgaac ctttaggatt acagcataca aaattttatg accaagtcaa
    34741 aaattcatcc aatcatgcac tatcctatca aatgtctaaa gaggatgatt actttaaacc
    34801 actgacagaa tctgaaacag agatccataa cgaattaggg actggtaata tcagtatgtc
    34861 cgtctatgat ttaaatacgc tgtttacaca agttttatct ggaaaactag tttccaaaga
    34921 cttactcttc tcattatggc gtgaaaatgc gaatggacat ccatatagtg gtggggtata
    34981 tagcggagat aattatatcc tgtcgcaagg aaatatcgac tcttttgata catctgctat
    35041 atttagcaaa gattcacagg atgctatcat catggaaagc aatataaaag ctgacaaaac
    35101 tatcaaactg cctactgctg atctaagaaa ccaaatctat gacctgatgg aaggaactac
    35161 taagttggca aatagctgat atgatgaaaa gatagcaaat actacttctt tgaatagcat
    35221 ttgcttaaaa aaagtactcg actaaacgta ggaaagagtg tgggacaaat ctaaaaatca
    35281 atttttgcta cagactttaa ttccaaataa acagaacaga aagaggtgtt cttctttctg
    35341 ttctgtccaa attattctaa gatttctctt taccaacatt atttgacaaa gggcctatta
    35401 aatctaaaat agtatcttta gattgctaaa atatgtgcta gcaaatcatt catggcttgt
    35461 tcttattttt aattttatgc aataaaaaag aagaacaatt agcgtgatta tagaaataaa
    35521 taatgagatt ttagaaaaaa atgtgtatga atacctgatt gttactttat ttattccact
    35581 ttcacttttt actgtaacaa aaggacctcc ttgttttagt actgtagcat tttttgaatt
    35641 aacagtaacc gttaatcctt tataccggaa tacaggtaaa actattgttt tgccttttga
    35701 agatttaaac gtaaatgtaa tttcatcttt atttggagaa aagctactat aaataacttg
    35761 accatcttct gtaacttgat ggtttttcaa aagcaattga tcttttttat agcctatagg
    35821 tacataatca aaattctcaa attcttcaag tttattttcc atttgttcat tagaataagt
    35881 tccttgaaaa ttcatttctt tcaccatgtt aactactgat gaataattta aaattgaaat
    35941 agctaatatt aataatagta taaaatttct tctccttgta taagaaaatt tttccaactg
    36001 tggagaaata aatagagata ctgaaaaaga tatgaacaat gttgaaaata tcataaaacg
    36061 ccaaggagct tgaataatat ttaaaggagt attttgtaag gcaaaccatg gaaatatttt
    36121 tgtacaaagt agaatagata ttatacccat tatataagtg attttagata agattcctaa
    36181 ttttttaaag ttaaatgtta gtataattaa acaaataagc aaagttaagc caataccaaa
    36241 tgatagtaat tctgaatcta tacttccaat caaaaactct ttacccagat aaattgctct
    36301 tttttgtaat atatccacat aaggtctatt cagatcttga tacaatgtct gctcaatcat
    36361 aggacctata aacgctagtg aaagaagtaa agtaactata gcagctttta ttaatttaat
    36421 aattaagaag atttctattt tctttttccg ataaaattga ataattaaag caattataat
    36481 cattaccata gtcatagcta cagaaagaac atgagtgtaa attaataaag tcatgctaat
    36541 cgccaacata acccaagatt ctttttttga gtcgtaaagc tttataaacc cgagtaaaat
    36601 aagaggaagt attgacattg cgagtaattc ccccattgcc cctcgaagaa atatttctac
    36661 actcctaaaa gaagatgttg tatatataat agcgaaaaag gagctagtaa catgtttttt
    36721 ggtaatttca tacattacat aatgacatat aaatagagta atgagcgtaa caatataaag
    36781 aaaaaggtta tagcctattg ctaaattagt aaacttgatc aataaaaata aaggatatag
    36841 agttaaccat gggtaaaaat tatttacttg catgccatat gatctaaacg tttcaaaatt
    36901 aataggggag ctaaaaatag attctaaact taacgcacga ttaatgtgaa atgccgtatc
    36961 ataaaagcca aatatttcca tgtttttaaa tgctggaaat aaatataata aactaacaaa
    37021 tgcataaact ataaatattt ttacagaaaa aatttttttc aaattcatat accacctcat
    37081 gtttttttat attacatata gtatcattta tttttttaaa acactagatt agtcactata
    37141 taaaatgtga ttaattttat tattaataaa atatttttta tattacaaaa gtagtgtttc
    37201 aaagttagta gagaaaatgt a
//
LOCUS       DAMYXM010000021        36552 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.022, whole genome shotgun sequence.
ACCESSION   DAMYXM010000021 DAMYXM010000000
VERSION     DAMYXM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.022"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            25..696
                     /locus_tag="PNS21_001233"
     CDS             25..696
                     /locus_tag="PNS21_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI5158950.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="PNS21_001234"
     CDS             755..1195
                     /locus_tag="PNS21_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158951.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="PNS21_001235"
     CDS             1207..1875
                     /locus_tag="PNS21_001235"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDI5158952.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="PNS21_001236"
     CDS             1939..2562
                     /locus_tag="PNS21_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDI5158953.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="PNS21_001237"
     CDS             2618..3004
                     /locus_tag="PNS21_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HDI5158954.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="PNS21_001238"
     CDS             3217..4608
                     /locus_tag="PNS21_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDI5158955.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5219..5587
                     /locus_tag="PNS21_001239"
     CDS             5219..5587
                     /locus_tag="PNS21_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDI5158956.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5767..6708
                     /locus_tag="PNS21_001240"
     CDS             5767..6708
                     /locus_tag="PNS21_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDI5158957.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6777..7508
                     /locus_tag="PNS21_001241"
     CDS             6777..7508
                     /locus_tag="PNS21_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDI5158958.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7652..9832
                     /locus_tag="PNS21_001242"
     CDS             7652..9832
                     /locus_tag="PNS21_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDI5158959.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9852..10334
                     /locus_tag="PNS21_001243"
     CDS             9852..10334
                     /locus_tag="PNS21_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDI5158960.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10350..11111
                     /locus_tag="PNS21_001244"
     CDS             10350..11111
                     /locus_tag="PNS21_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDI5158961.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11242..12576
                     /locus_tag="PNS21_001245"
     CDS             11242..12576
                     /locus_tag="PNS21_001245"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDI5158962.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12883..13368
                     /locus_tag="PNS21_001246"
     CDS             12883..13368
                     /locus_tag="PNS21_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDI5158963.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13361..14044
                     /locus_tag="PNS21_001247"
     CDS             13361..14044
                     /locus_tag="PNS21_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDI5158964.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14170..16815
                     /gene="polA"
                     /locus_tag="PNS21_001248"
     CDS             14170..16815
                     /gene="polA"
                     /locus_tag="PNS21_001248"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDI5158965.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16860..17696
                     /gene="mutM"
                     /locus_tag="PNS21_001249"
     CDS             16860..17696
                     /gene="mutM"
                     /locus_tag="PNS21_001249"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HDI5158966.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17660..18289
                     /locus_tag="PNS21_001250"
     CDS             17660..18289
                     /locus_tag="PNS21_001250"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDI5158967.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            18341..18673
                     /locus_tag="PNS21_001251"
     CDS             18341..18673
                     /locus_tag="PNS21_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDI5158968.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18985..19476
                     /gene="nrdR"
                     /locus_tag="PNS21_001252"
     CDS             18985..19476
                     /gene="nrdR"
                     /locus_tag="PNS21_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDI5158969.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19499..20872
                     /locus_tag="PNS21_001253"
     CDS             19499..20872
                     /locus_tag="PNS21_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDI5158970.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20872..21798
                     /gene="dnaI"
                     /locus_tag="PNS21_001254"
     CDS             20872..21798
                     /gene="dnaI"
                     /locus_tag="PNS21_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDI5158971.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21927..22367
                     /locus_tag="PNS21_001255"
     CDS             21927..22367
                     /locus_tag="PNS21_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDI5158972.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22511..23212
                     /locus_tag="PNS21_001256"
     CDS             22511..23212
                     /locus_tag="PNS21_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDI5158973.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23209..24330
                     /locus_tag="PNS21_001257"
     CDS             23209..24330
                     /locus_tag="PNS21_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDI5158974.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24345..25577
                     /gene="pepT"
                     /locus_tag="PNS21_001258"
     CDS             24345..25577
                     /gene="pepT"
                     /locus_tag="PNS21_001258"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDI5158975.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25709..26617
                     /locus_tag="PNS21_001259"
     CDS             25709..26617
                     /locus_tag="PNS21_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDI5158976.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26652..26924
                     /locus_tag="PNS21_001260"
     CDS             26652..26924
                     /locus_tag="PNS21_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDI5158977.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26935..27981
                     /locus_tag="PNS21_001261"
     CDS             26935..27981
                     /locus_tag="PNS21_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDI5158978.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            28232..30841
                     /gene="clpB"
                     /locus_tag="PNS21_001262"
     CDS             28232..30841
                     /gene="clpB"
                     /locus_tag="PNS21_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDI5158979.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30992..31678)
                     /locus_tag="PNS21_001263"
     CDS             complement(30992..31678)
                     /locus_tag="PNS21_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5158980.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31656..32150)
                     /locus_tag="PNS21_001264"
     CDS             complement(31656..32150)
                     /locus_tag="PNS21_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDI5158981.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(32170..33390)
                     /locus_tag="PNS21_001265"
     CDS             complement(32170..33390)
                     /locus_tag="PNS21_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5158982.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33537..35372
                     /gene="lepA"
                     /locus_tag="PNS21_001266"
     CDS             33537..35372
                     /gene="lepA"
                     /locus_tag="PNS21_001266"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDI5158983.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(35466..>36552)
                     /locus_tag="PNS21_001267"
     CDS             complement(35466..>36552)
                     /locus_tag="PNS21_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDI5158984.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        12166 a         6464 c         7816 g        10106 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacaga tcgtttagga ggaaacagat
     5221 gtttacgaat gaaataaaaa tcatgcttta tgtggacgat gtagaaaaaa atgcaacgtt
     5281 ttggagagca gttggctttg cggaaaaaga tcgtcaagaa atggatggga cgcttataat
     5341 tgaagtagga gttgaggaca gtcaaacctc atttctgtta tatgatcgag catttgttga
     5401 gtcccattca cctggcacag ctactagtgc cccatctttg atgttttaca gtaaagatat
     5461 ctttggacta tataaaaaaa tgcagacgca atcagtcaca ttaggtgata tggtacagct
     5521 tggagaagaa tatgtcttta actttgctga tcccgatggc aactactttg cggttacagg
     5581 taaataatag aaataaagac tttgtttttc tgtgcgaaac aggaaagcga agtcttttta
     5641 attcaaataa taatattatg taaacaaaaa actttccatg cgtaattata cacaagcaaa
     5701 taaatcgtca aatgcgaacg ccttctttat actgtttagg aacgaaaaga aggaaggaag
     5761 aaatacatgg attatcaagt actgctttat tacaattata caaaaatcga agaaccagag
     5821 gaatttgcca aaaatcattt agccttttgc aaatctttgg atcttaaagg tcgtattcta
     5881 gtagctgaag aaggaataaa tggtactgtg tctggaaccg ttgaaaacac gactgcctat
     5941 atgaaagcaa tgaaagctga tcctcgattt gctgatacga tgttcaagat cgatccaagc
     6001 gaaggacatg cctttaagaa gatgtttgta agataccgtc cagagcttgt aagtttaaag
     6061 ctagaagagg acattgatcc aaaccaagtg acaggtgcct atctttcacc gcaagaattt
     6121 aaagaagcta ttttagatga acagacagtg gtgattgatg cacggaatga ttatgaatat
     6181 gacttgggac attttcgagg ggctgtacgc ccagatatac gcagttttcg agagcttcca
     6241 gactggatcc gcgagcacaa agaagaattt atggaaaaac gggtagtgac ctactgtact
     6301 ggtggcattc gttgcgagaa gttttctggt tggcttgtcc gtgaaggatt caaagatgtc
     6361 ggacagctgc atggcggtat tgctacttat ggtaaggatc ctgaagtcca aggagaatta
     6421 tgggacggca agatgtatgt gtttgatgaa cggatcgctg tcgatatcaa ccaagtagat
     6481 aaacaaatcg tcggtcgcga ctggtttgac ggaacaccat gtgaacgata tatcaattgt
     6541 agcaatcctg agtgcaatcg tcagattctt gcttccattg aaaatgaaga aaaacattta
     6601 ggagcatgta gcatagagtg tgcgaaacat ccgcgcaatc gctatgttca agaccatgga
     6661 atctctccag aagaagcaga actgcggttt acagctttaa ttaaatagaa aacaatagtt
     6721 ttgattgtcg accatttcca atatgcgcta tactagcgtt gaggatggtg atccacatga
     6781 aatcattgat ttatgctatt ggggatgtcc atggcaaaca tgagcttttt gaaaaactta
     6841 ttcactatta tgatccaaag atccatcagc ttgttttatt aggggatctc aatgatcgag
     6901 gtcctcgtac aaaagaatgt tttttattag gaaagcaatt agtcgaagaa acaggagcca
     6961 tttacctccg gggaaaccat gaagaatatt ttttgcaatt tctgcaatca ccggaggact
     7021 ggtatgcgcc ttatatgaga aatggtggaa aagaaacgtt tgaaagcctg attcacccag
     7081 gggcatcgga ggaatactcc ccaactgaaa tctcgatgat gatccaatcg agatattcag
     7141 aactgattaa atttttagtc cagcgcccct tgtattatga atgggaaaat tatctttttg
     7201 tccatgccgg tgtggatttg acaaagaaag actggcgtaa gacaagtcta agagacttta
     7261 tttggataag ggaaccattt catgaaggga aaaacaatac aggtaagaca attgtatttg
     7321 gacatacgat tacaccaatg ctccatggag atatgcagac aacagacctc tggattcagg
     7381 atcataaaat cggaatggac ggtggggctg tattcggagg gtcattgcat ggggttgttt
     7441 ttgacaagga aggtattctt caagatattg aatatcagaa caacgaagga ccttggcatg
     7501 gaatctgact aatggaaaga atgaaggagc tgtgactttg ttgcggctct ttttattttt
     7561 atagagaata ttgtttcttt cagtagttta acccaggata aaaagtatga tagaataaag
     7621 aagcaatttt tagcagaaaa gaggcatttt aatgacggaa aatttgaacc agacgatcat
     7681 tcagcttgta caaaaagaac tttcagacta tcgtcctaag cagttgacta ctgtacttaa
     7741 tttgttaaat gaaggaaaca ctgttccttt tatcgcgcgt taccgaaaag aaatgactgg
     7801 aagtcttgat gaagtccaga ttcgtgaaat cgaagagcgg tatgcctatc ttgaaaactt
     7861 agaaaaacgt aaaactgaag tgattcggct aatcgatgaa caagggaaac tgactcccga
     7921 gttagaagca gaaatcaccc aagccgtgaa aatgcaacaa gtggaagatt tgtatcgtcc
     7981 atacaaacaa aaacgacgga caaaagcaac gattgctaaa gaaaaaggac ttgaaccact
     8041 cgcgaagtgg ctgatgcagc ttacagatgg agaggtgcaa tctgaagcag aaaaatatat
     8101 tgacaaagaa aaagaagtct cttctgccga agaagcgttg cacggtgctc atgagattat
     8161 tgctgaacaa gtcagcgata atgcaaaatt tcggacttgg attcggtctt atacgtataa
     8221 caaaggtatg tatgtcagca atgtcaagga tgagcaagca gatgaaaaag gcgtatatga
     8281 aatgtattat gactttgcag aacccgtcca taaaatggtt tctcatcgaa tattagctac
     8341 taatcgtgga gaaaaagaag atattctgaa agtattcttt caagtagatg aagcagcaat
     8401 tttagcttac ttggatcgcc aaatagtcaa aaatcctgcc tctccttctt cttcattcgt
     8461 cagagaagcc tatcaggata gttataaacg ctttattcag ccagcaatcg agcgagaact
     8521 gcgcaatgaa ttgactgaaa aggcagatga gcaagcaatc gctattttcg gcgaaaacct
     8581 ccgtaatctt ttgttgcaac cgccactaaa aggtaaagtt gtcctgggat ttgatccggc
     8641 ttatcgtact ggatgtaaac tagcagtcgt agatgcgaca gggaaagttt tagcaatcga
     8701 agttatttat cctcataaac ctgcagcgca agcaaaaaga gaagctgctg gaccagcttt
     8761 tatccaatta attaataaat atcaagtaga tatggtagct atcggaaatg gtacagccag
     8821 tcgggaatct gaactttttg tggctgagca gttgaaatcc gccgaccata aaacctatta
     8881 cgctatcgtc aacgaagcag gcgcttctgt ttactccgct agcgaaattg cacgaaaaga
     8941 atttccgcat ctacaagttg aagaaagaag tgctgtttct attgcccgtc gcctgcaaga
     9001 tcctttagct gaattagtca aaatcgatcc taaagctgtc ggagtaggcc agtaccaaca
     9061 tgacgtttct caaaaacgtt tggctgaaca attggatttt gttgtggaaa cagcggttaa
     9121 ccaagtaggt gtggatgtga atacagcaag cccgcagctc ttgcagcata tatcaggatt
     9181 gaataaaaca actgcccaaa acattgtcat ttatcgtgaa gaaaacggag aattcaccgc
     9241 acgaactcaa ttgaaaaaag tgccacgctt aggaccaaaa gcatatgaac aagcaatcgg
     9301 attcttgcga gtccctggtg ggaaaaacat tttggacaac acaggtatcc atcccgaaag
     9361 ttattctatt gcaaaagaaa tattaacttc agtccaactt tcagaaaaag agcttggtac
     9421 agaagaagca gtagaaaaat tgacacgatt atccgtagag aaactggctg aaagcctatc
     9481 agtcggagaa gaaacgttag ccgatatttt agctgggctg acacaacctg gacgagatat
     9541 gcgtgatgag atgccagcgc ccttattacg tacagatgta ttgagtatgg aagacttgaa
     9601 accagggatg gaattgactg gaacggtccg taatgtcatt gacttcggcg catttgttga
     9661 tatcggtgtg aagcaagacg gacttgtcca tatatcgaaa ctcagcaaaa aattcgtcaa
     9721 gcatcctaca gatgtcgtgt cagtaggaga tatcgtaaca gtttggatag aacaagtcga
     9781 tacgaaaaaa ggtagaatca gtttgacgat gctttcccct tatgaagagt aacaatgagg
     9841 agtaacaaaa attgacacaa gaagaactgc agcagttagt agaagagata tcattaaaaa
     9901 gcttccagct tcctttcaag caccaagcaa tgttcaatcc acgcttacgt acaactggcg
     9961 ggcgctatca tttaaacgat catcatttag attttaatgc gaagctattt gaagagtcag
    10021 acgaagagac gattgcagga atcatcaaac atgaactttg ccattatcat cttcatctag
    10081 ctggaagagg gtatcagcat aaagatatgg atttcaagta tcttttgaaa aaaacaggag
    10141 ggttgcgcta tgcaccagcc ttgacgaaga aacagataaa gatggagtat tatcgatgcc
    10201 aagattgttc tgccacgatc tgccgcaaga gaagaatcga tgtacgcaaa tatcgttgtg
    10261 gaagatgtca tggagaaatc aaatggattg agacaaaaag agaagacttt aatgaagcac
    10321 aaaaaatcag atgaaaggat agaaggcctt tggaaaaaag tgagcagagg aatctaaaag
    10381 actggaatca gctattaaat tttttagccg cacctgaaga gggaaatttt gaggaactga
    10441 cggcacctac acttatttta gctggaaact gtcttcctat cttagctgat aaagcagcac
    10501 aaatgtattt aaatggccaa gtagatcaat tgtttttggt cggaggggtg gggcatgcga
    10561 ctagaatcct ttatgagaat tttgaaaagc aaggatttca ttttgaagat ggcatgagtg
    10621 aaagtgaaat ctgtcgtcag tatctaaaag aagtctatga tttgcccgat aaggcttttc
    10681 tgatagaatc gaagtctaca aattctggag agaacgccac tttttcactg gagattcttc
    10741 attccttagg agctgtacca gaaaaaatct tattgatgaa tgatccgaca ctgcaacgca
    10801 ggactcgagc aacttttgag aaagtatggc aaaatgaaca aactattttc gcgaatgctg
    10861 tgccatttgt gccggaaatc cttcactttt ctgaagagat catctttaca gcaaaagaac
    10921 tgaatcatca atggcctaag gaatatttcc atgcgctagt attaggagaa atggaacgtc
    10981 tccatgacga tgagaacggc tatggtccga aaggaaaaga ttttattcct cacattgata
    11041 tatcagagga agtttggtct tcttacacca gaattaaaga gtcaataaaa acggattttt
    11101 ctcgtacttg accattttag caagcgaatc ctcctttcca aataaatttg gttgaaattt
    11161 tcaatcgttt ataagataat gaggagcgtg ttgaatgtaa tagctaaaag aatggacgct
    11221 agataagaga agaggattca aatggaaaat caaaataaat tgtatcattt tgttggtatc
    11281 aaaggttcag gaatgagctc cttggctctt gttttacatg aacaaggtct aaatgtccaa
    11341 ggatcagaca ttgaaaaata tttctttaca caaagagatt tagaaaaagc aaatatcacg
    11401 attctgccat ttaacgcaga taatgtaaaa ccagggatga cgatcattgc aggaaatgca
    11461 tttcctgatt cacatgaaga aatccagcga gcaaaagaat taggtttgga agtgattcgc
    11521 tatcacgact ttattggtca ttttatccaa aattatacga gtatcgctgt aacaggctct
    11581 cacggaaaaa caagtacgac tggactgctc tctcatgtat tgagtggtgt ccgtccaaca
    11641 agttatctaa ttggagatgg aacaggacat ggtgatccgc aagcggaatt cttttcattt
    11701 gaggcctgtg aatatcgccg tcatttcctt gcctattcac cagactatgc aatcatgacc
    11761 aatatcgatt ttgatcatcc ggattattat acaagtatcg acgatgtatt tacagctttc
    11821 caaacaatgg ctggacaagt gaaaaaagct atttttgcgt atggtgacga tgcttattta
    11881 cgcaaattaa aagcaaatgt gccgatttat tattatggtg tcacagaaaa cgatgatatc
    11941 caagcacgaa acatcgaacg cacaacttct ggttctgctt ttgatgtcta tcatggggat
    12001 gaatttgttg ggcacttcac tgtaccagca ttcggtaaac acaatatctt aaatgcttta
    12061 ggtgttattg cagtagctta tttcgaaaaa ctggatctga aagaagttgc agaagaaatg
    12121 ctgactttcc caggagtaaa acgtcgattc agtgaaaaga tcgtagcaga catgaccgta
    12181 gtcgatgatt atgcgcatca ccctgcagag atcaaagcaa cgattgatgg cgcacgccaa
    12241 aaatatccag ataaagaaat aattgcagtt ttccaacccc atacgtttac gcgtacgatt
    12301 gcattgatgg atgaatttgc agaagcactt gatttagctg ataaagttta cttatgcgat
    12361 atttttggtt cggctcggga agaacaagga aacgttaaga ttgaagacct tggagcgaaa
    12421 atcaaaaaag gtggagaagt catcaaagaa aataatgtat ctccattgtt agattatcat
    12481 gatgcagtag taatcttcat gggtgctgga gatgttcaaa aatttgaaca agcgtatgaa
    12541 aaacttttaa gcagcacgac gaaaaatgta ctgtaataca attactgcag tgtccttata
    12601 aaaatagaaa gagggggagt ggcaaaaatc ttgccaagcc cccttttttg caacttatga
    12661 taaacggtgt tcaaaagctt gcatctgcgg taataagccc aatcaaatca aaaacatgag
    12721 aagctgtttt ttcttggctg ttcttattat ttgatgcagg acagcttttt cacgacctct
    12781 gtaaactata ggaatctgtt aaatcaaggg agcagaactt gccttttacg ctacttttgg
    12841 tacaataatg tatgattaaa aataaactgg agggtttcta tcttgaacat aggtaagccg
    12901 agaaaactag gaaaaacaat aaatgaaatc gaggaaggcg actcgttatc cttaacagaa
    12961 tctattgaag ataaccagct tttgctgtat ttgggattga cgaatgatgc aaatcctttg
    13021 tatatccaac atgactttgc acaaaaaaca gaatatcaac agccaatcgt tccatcagtg
    13081 atgctgatgg ggatcatcac aagtgcaatt tcaaagcatc ttcctggacc aggatcacat
    13141 gttgtcaatt tttctgtcaa ttttattgaa ccggttttcc attatgaaac actgactttt
    13201 cagtttgagg tcataaaagt agataagatg aaggatgtgg tgacgatttc tgtagaagcg
    13261 gagaatacga acgaagatcg tgtgttggat gctgtcgtta tggtccaacc gccacaagtt
    13321 cctgaattgg aaatagcaat cgaaagtgaa ggtgaattga atgaataatc caagtacatc
    13381 aatgtcaggt atcaatcgtt tttatgcaaa agtttatgga tttttaggta tgggacttgc
    13441 ccttagtgca gtcacttctt acttggctct ttgggtattt cctttgcaat tagcaacttt
    13501 tatcaataat tttccactag gctttgtggg tctgtggatc gttgaactga ttttagtaat
    13561 ggttctaggc gtaaaagcac aaaagaatcc gagcttagca atcggtggat ttattgccta
    13621 ctctcttttg aatggcataa cattagcggt tactcttgca tactacgata ttggaacagt
    13681 aacgcaagct ttcttgacag ctactttgat gttcatgggg atgtctgttg tcggagtaac
    13741 aacaaaaaga gatcttagcg gggttggaag agcaggacag gcagctttaa tcggagtgat
    13801 cattgcacta gtcttgaaca tcttcatttt gagaagtagc gtagttgagc tgttcatctc
    13861 cctactgtta gtcgtcatct ttgcagggat tacagcttat gataaccaaa gcatccgtaa
    13921 tatctatgtc cagacaaacg gaacagcaca tagtggaatt gctgtattca tggcattgaa
    13981 gctgtatttg gactttatca acttgttctt ggcgtttctg agaatctttg gacggaacaa
    14041 ctaaaaaaga aaaaaattca tcagttgggg ctataccgaa ttatttggta tggtcccgct
    14101 ttttcttttc gcattccgca ggattctttg ttagaatgaa taagaacttt acagtaagga
    14161 gttaagaaga tgacaaaaaa taaattatta ctcgttgatg ggaacagtgt ggcttttcgc
    14221 gcattctttg cgcttcacaa ttctcttgaa cgtttcaaaa atagaaacgg tcttcataca
    14281 aatgcaatct atgcattcaa tacgatgttt gaaaatgtta tgcaaaaaga gcaaccgact
    14341 catgttctgg ttgcgttcga tgcaggaaaa acgacttttc ggacagaaat gtatgcggag
    14401 tacaaaggtg gaagatccaa aacacctggc gaattcaaag aacaaatgcc atatatacgt
    14461 gatttgttga caggactagg ggtacagtat tacgaattgc ccaattatga agcagatgat
    14521 attatcggta ctttggcaga aaaagtagac aaagatcaat ttgatgtcgt cgtgctttca
    14581 ggtgatcggg atttgactca attagctagt aaagaagtaa aagtggatat cactgtaaaa
    14641 ggtgtcagcg atatcgaaag ctatacacca gaacatgtag cggaaaaata tgatgggttg
    14701 acgccaaaac agattattga catgaaggga ctagccggag atgcctctga caatatcccc
    14761 ggtgtgacta aaatcggtga aaaaacagct atcaagctat tgaaacaata tggctcagtc
    14821 gaaggaattt atgaacatat cgatgaaatg aagcaaagca aaatgaaaga aaatctgata
    14881 aatgacaaag agcaggcttt cttatcgaaa aaattggcga ccatcaatac ttcagcgcct
    14941 gtggatgtct ctatcgactc attgaagtat gaagggaaaa atctcgataa actcgttccg
    15001 ttttataaag aaatggattt caatcagttc ctttctaaat tgaatatcgt agaagaagaa
    15061 gtaaaaatgg atgatatttt gtttgaagtc gttcatgaat tcaaagagga aatgtttact
    15121 acggatatgg cgttgtacgt ggaaatgatg ggcgacaatt atcatacaga ggaaatcgtc
    15181 ggagtagcgt gggggacaga aaagaaaatt tatgtcacga acgatctatc tgtattcgaa
    15241 aaccgagcgt tccattcttg gatcactgat ccgactcggc tgaaaaaagt atatgatgcg
    15301 aaacgaactt atgttgcttt aaaccgctat actggaaaaa cgaaaggaat cgattttgat
    15361 gtcttgctag gtgcatacct tctcgatacg aatgaaaaaa gcacagatat cgaaggaata
    15421 gctgctcatt acggatacaa cgatatccaa tcagatgaag ctgtatacgg caaaggggcg
    15481 aaaaaaggtt taccagaaga agaggaactc ttttttgctc atttagcgcg aaaagtagct
    15541 gcaatcaatg cgttgacacc aaaattgagc caagaactgg cagaaaaaaa ccaagaagat
    15601 cttttccgga aaatggaact gccattgtct atcattttgg cagagatgga aataagcggg
    15661 atcaaagtag atgcgactcg cttacaagaa atgaagggcg agttctctgc tcgcttaaga
    15721 gagatcgaac aaaaaatcta cgaagaagcc ggcgaagaat tcaatttgaa ttcaccaaag
    15781 caattaggag tgatcttatt tgaaaaaatg ggattacccg ttatcaaaaa aacaaaaact
    15841 ggatattcca cagccgtaga tgtactggaa cagcttcgtg aacaagcacc aatcgtagag
    15901 gatatcctca cctatcgtca aattgcaaaa attcaatcta cctatgtcga gggactgctg
    15961 aaagtgatcc aatcagatgg aaaagtgcat acccgttatg tccagacatt gacccaaaca
    16021 ggacgattaa gctctgttga tcctaatttg cagaacatcc cgattcgatt agatgaaggg
    16081 agaaagatac gtcaggcatt tgttcctaga gaaaaagact ggctgattta ttcttctgac
    16141 tattcacaaa tcgagttacg cgtacttgcg catatctcaa atgatgaaca tttgaaagct
    16201 gccttcctag aaggtcaaga tatccatgcc agcacagcga tgcgtgtatt tggaatcgaa
    16261 aaggcagagg atgtcacacc aaatatgcgt aggcaggcaa aagcagtgaa ttttggtatc
    16321 gtgtatggca tctctgatta cggactttca caaaatctag gaatcagccg aaaagcagca
    16381 cagcaataca tcgatacgta ctttgaaaaa tacccaggtg tcaaagaata tatggaaaga
    16441 atcgtacgcg aagcaaaaga ccaaggctac gtcgaaacgc tgtatcaccg ccgtcgatat
    16501 ctgccagaca tcaattcaag aaattacaat atccgttctt ttgcggaacg aacagctatc
    16561 aatacaccaa tccaaggaag tgctgctgat attttgaaaa tcgcgatgat cgaattggat
    16621 aaacgattaa aagaaacagg tcttcaagct acgatgctgt tacaagttca tgatgaactt
    16681 gtcttcgagg taccggaaaa agaacttgaa tcattagata aattggtgaa ggaagttatg
    16741 gaacaagctg tttctcttca tgtaccactg atcactgaca gcagttgggg aaaaacgtgg
    16801 tacgaagcaa aataaacatt gtttaagaca gagcaatata atgaaggtga aaagaaaaaa
    16861 tgccagaatt accagaagta gaaaccgttc gtaaaggatt ggaacggtta gtagtaggaa
    16921 aaacgattca aaaagttcag gtcttgtggc ccaagatcat cgagcaacca gagacaccca
    16981 tctttgaggc ttctcttgtt ggcgaaacca tccaatccat tggacgtaga gggaagtttt
    17041 taatttttca tctcgatcat tgtgaactga tctcacactt gcgaatggaa ggaaaatatc
    17101 aatttacgaa agaaaataca ccaatcgata aacatacgca tgtacttttc ttttttgagg
    17161 atggaagcca acttcgctac aacgatgtac gaaaatttgg tcgtatgaca atcgtcgaaa
    17221 aaggagcaag tgcaacttac cgaggaatca tgaaattggg acctgagcct ttgccagata
    17281 gcttcttatt agcagatttc gcaaatggac tgaaaaagtc ccacaaagca atcaagccat
    17341 tgctgctaga ccaacgtcta gtgactggtc taggtaatat ttatgtagat gaagcattat
    17401 gggaagcgaa gatccatcca gaacaaccgg cgaacacact gcgccccaaa gaggtagaac
    17461 aattacgtct atcgatcatt gatgttttgg atcgtgcaat agaagccgga ggtaccacga
    17521 ttcgcagtta tttgaatgca ttaggagaat caggcggatt tcaagtagca ctgcatgtct
    17581 atcagcaaac tggcaaacca tgtatacgtt gtggaacgcc gatcgtgaaa acaaaagtgg
    17641 cacaaagggg gacgcactat tgtccgaatt gtcaaaggct aaaaatcgca aagtaggatt
    17701 tgttttaggt ttgacgggtg gaatagctac cggtaaaagt acggcagcca aagtctttca
    17761 gtctcatgga tttcctttga tcgatggcga tgtgattgcc agagaaactg ttgagcctgg
    17821 aacgcttgct ctgaggaaaa ttgtatcagt gtttggccaa gaggtattgc agcctgatgg
    17881 gcaactagat cgagggaaac ttgggatgat tgtttttcca tcaaaggaaa aacggcaaaa
    17941 acttgatcag ctattagatc cgtttatccg aaaagcgata aaagatcaaa tcgcctctct
    18001 aagttccaat catcctttag taattgtaga tatcccattg ctctacgaag gtcattatga
    18061 ccactatatg gatgcagtag ctgtcgttta tactacgcca gaaactcagc ttcagcgttt
    18121 gatgaaacgt aatcaactga cgctagaaca agcgcagcag agaattagta gtcaacttcc
    18181 aatcgaagaa aaaaagcagc gtgcggatat tttgttcgac aacaatggaa caaaagaaaa
    18241 acttgtggga caaatcgaaa attggctttt tgctaatcgc tttctttgag tatctgtgtc
    18301 aattttgcta tcctgagtat atagaaagtg agggatagcg atgggatcat taaaaaatca
    18361 aaaggataaa gtcgtaggta acgcaaaaga aaaaatcggc gagatgctca ataaagaaaa
    18421 tctaatcgat gaaggtcgta cgcaatcaga aacagcagaa gaaaaagaaa agcagtacca
    18481 agcaagaatc gatgaaatag aagaccagca ggcagaagaa gaagcgaagc ggcaagattt
    18541 gactcaagaa cgaaaaaatc cgacacctga tcgtagtaaa acaccgctcc cagaaagtga
    18601 ccacattccc gatgagcaaa aaatcgagga acagcgaatg aaacactata caggaatcga
    18661 agagaagtta taaaatagta aaaaatcgag atgtgacttg aagcagatga gaaggtgttc
    18721 atctgcttca agttttttgt gctagatgac taaatcagct tttgtgtaag ttatttctca
    18781 gtaatcaagg tgatcagtat tggaaaattg aaatttagta aggacagggg acacaattac
    18841 gcatgaaagt agaaaaaatt gttcttatat ccgtgttttt tgggtaggac agtcgcgttt
    18901 gtgatttgtt tcgtgttata atacaactag ttgtaaagaa agacacatcc tttatataaa
    18961 atgcgatgca agaggtgaga caaaatgaga tgtcccagat gtcaacataa taattcacga
    19021 gtaatcgaca gccgtcaagc agatgatgga cgtgcgatcc gtcgccgtcg cgaatgtgag
    19081 aattgttcgt tccgattcac tacatttgaa cgaattgaag ctgctccgtt attagtaatc
    19141 aagaaaaacg gggaacgcga agagttcaat cgcgataaaa ttttaagagg actcatccgt
    19201 tcagcagaaa aaagacctgt agctatggaa caaatggaac aaatcgtcaa ccatgtcgaa
    19261 aaccgcgtac gcagtctcgg agaaaacgaa gtatcgacga atctaattgg tgaatatgtg
    19321 atggaagatt tagttgattt agacgaaatc gcttatattc gttttgcaag tgtttatcgc
    19381 cagttcaaag atatgtctgt tttcttaaaa gagttgcaag atatcgtgga taaagcaaaa
    19441 gaaaataata aagagaataa taaagaaaac aattaagaaa caaaaaggag gcatttcctt
    19501 ggaaaatgct tggaaagagc ttcaaccaaa aaatatttac caaggaagaa aagcggctcc
    19561 gctgagagaa gacagcaaga acgccttgct gtatctttat cagccactaa tcggcggaga
    19621 agccttgagt atctatttca ccctgttatc agaaatctct caagaatctg gcaaaggtcc
    19681 tgaagggatg catgcagatc tgcttagtag tctaggctgc ggtatcccgc aattttatga
    19741 tgcaagaaaa aagctagaag gaatcggctt actggatgtt tattttaaag aagatagtga
    19801 tctgggacct cgttttattt atgagctgtt agaaccactc attcctaaag catttttcaa
    19861 agactctgtt ctggcttttc ttcttctgga aaaagttggt gaacgacgat tccagaagct
    19921 tacccaattc tttgaaccta aaaggcatac agcagaggga tatcaaaaaa tcacaaaaaa
    19981 atttttagat gtttatcgct tcaatgaagc agtgtacgct gctaatcaag aaaacatcga
    20041 atcgctgaat aaatcttttg aagaatctga atcagtagca ttacttgaag cagattcgac
    20101 attcgattgg cagtttttga atgattggct ggcaaaaaaa catattgcaa gtcttgatga
    20161 tccaatcatc aaacaagtcg acatgtatca tcagctttac ggtttcgatg aattgacact
    20221 tggcgaactg atcagtcaat cttatgactt tagtgaacaa aaagtatctg aaaaagaact
    20281 gcaaaaagcc attttggcaa tgcagagacg tccggtgaag caaacaaaag aaacagttgc
    20341 ggtttctgaa aacagtaatg aaccctcgtc aaacgaacat ttgacaaacg cagcgcttca
    20401 gctgataaaa gaagcacaga gcattccacc gatgaaatat ctggaagcaa tcaagtcaga
    20461 aaaaggcgga tatgtttcta aacaagaaaa ctggttactg caagatcttg tttctcaatc
    20521 aggtctttcc agtagcgtga tcaatgtttt gctgaattat gtactagtga tcaagaataa
    20581 tgctagctta aatgccagtt atgtaaatac agtggcaaat gaatgggcac aaaagaaaat
    20641 cgccacagca gaagaagcga tccagcacat tcggcaaata agtaatcaag caaaacaatc
    20701 aaaacaaaag aagcaaacaa attatcaaac tggcaagcga aatgttcgac gggaaaagct
    20761 tcctgattgg gtcaaccagc caaaagatga aaaacagatc agcagcgaga aaaaagcaga
    20821 gattgaccgc cgttttaaag aatacctggc aaaaaaggag ggtgatatct agtggaagac
    20881 gtaggcaaag aattgaagag gatcatcagc aaacgcaatt atcaagaaaa gtatgctgaa
    20941 atgatcgctg aagtcttaaa tgatgcggat gttcaagctt ttctaagcgc acaccaagaa
    21001 gaattgacac aagcggatat tgaaaaaagt tacgctaaat tgtatgaatt cgtgcaagaa
    21061 aagcgtaaat atcaaaaaaa cgatccaacg atgatcgctc ccggctatga gccgaagctg
    21121 acgttgaatt ttcattatgt ggatgtcact tatgtaccaa cggaagcatt gttagcacgg
    21181 caaagacaag aagaaatcag aagccgagta aaagcaatgg atatgccaaa agatattcaa
    21241 tcggcttctt ttgaaaactt tgaacgtaca gatggacgag gatatgcgag cttagaagcc
    21301 ctagattttg tagagaaata tactgctgat ccaaaagggt atcataaagg actgtatcta
    21361 gtaggaagtt ttggtattgg aaaaacatat ttattaggag caacagcgag agaattagcg
    21421 attgctggat tcaccacgac gctagtccat tttccgacat ttgccgttga gatgaaacag
    21481 gcaatcggaa aagatcaagt ggcagaaaag ttagatgcag tgaaacgttc acctattttg
    21541 atgatcgatg acattggtgc agatgcgatg agtagttgga tacgagacga agtattcggt
    21601 gttatccttc aatatcggat gcaggaacag ttaccaacct tcttctcatc gaattttaca
    21661 atggatgaat tggaacagca tttgtccgtc actcaacgtg gagatgaaga gccgctaaaa
    21721 gcgaaacgga taatggaacg catacgttat ttgacaaaag aaatcgaaat gactggtcgc
    21781 aaccgacgca atggttgaac agttgacaag tccaactgac atttgaccta gtagtaaaag
    21841 caggtcagcg aatcattata tggacagaaa tgaccggaga caatcaccgc aacaagtaat
    21901 gcagaaactt aaacggagga ttttttatga gggctatttt ttctagtata tctgtagttg
    21961 gtctattttt tcttttagca ggctgcggta gcgcagatag caacacaggt tctgttgatt
    22021 cgattgatgc gggtcaaaca gtaaaaacaa gcagtacgaa aacggagaga caaagcatat
    22081 ttgtcggaac gattgaaagt atcactcaag tagaagcaga agaagaaacg cttcagttga
    22141 aactcgtcga tgtcatggcc aaagaagatc cagcgaatat gggaacgtca ttcagcaatg
    22201 acggagtgat cttaaatgct actgctgaaa aaataagcgt ggacataaat gaattgaaaa
    22261 tcggtgacaa agtccaattt acattagttg agatgccgat catgacgatg tctatcccac
    22321 cgcaagttcc tggagattcg attattgatg tttcattgaa caaataacca ataacatttt
    22381 ttcaaaaata agaaaatgtt gttttataga acgaaaaggt atatttttta aggagatatg
    22441 ggaatttttt tattcctagt ctccttattt tatttggaaa atgctataat tatctagaag
    22501 gagtgaagct atgaaccatt tagatttatc tcagcgctta gccgcagtgg gtgactttgt
    22561 ccctgaatcc gctcgtttag cagacattgg atcagatcac gcttacttac ccgttgcatt
    22621 gatattaaga ggaaaaatcg aatatgcagt tgctggtgaa gtcgttcaag gtccttatga
    22681 atcagcacgt aagcaagtca aaaaagatgg attagaagac cgtatcaccg tacgccttgc
    22741 aaatggtcta gatgctatcc agccacaaga tggtattaca gctgtcacga ttgcgggtat
    22801 gggaggttca ctgatccgag atatcctaga agctggcaag ttgaatcaac gcttaagcgg
    22861 acaagaacgt ttgatccttc agccaaacat cggagagcgt acattacgat cttggctgca
    22921 agaaaatcag tataaaatcc aatcagaaaa aatacttgaa gaaaataaaa aaatatatga
    22981 aatcattgta gccgaaaaag aacaaaaacc agttacttac aatgaaatgg agctgatgtt
    23041 cgggccattt ctgcttgaag agaaaagtga gatcttcaaa tcaaaatgga caagagagct
    23101 tcaacaaaga aaagctatcc gtgaacagct caaacaagca gctaatcaag aggcacgtca
    23161 aaaacaaatc gagcaggaaa tccaaatgat tgaggaggtt ttaaagccat gacaatcagc
    23221 ggacgcactt tcatccaaaa gtttgaaagc tattgtcctc agtggctagc tgaagaagga
    23281 gacccagtcg gactgcatat cggaacattg gataagccaa tccaacgagt aatgatgaca
    23341 ttagacgttc gtccagaagt ggtcgaagaa gcaatcaaga aaaaaatcga tttgctgatt
    23401 gctaaacatc ctccaatctt tcgtcctgta aaacggctgg ttactgatca gccacaagaa
    23461 aaaatgtatg cagatttgtt gaaacatgat attgccgtct atgcagcaca cacgaacatg
    23521 gatattattt gggatggact gaatgactgg ttttgtgaac ttctcggtat tgaagtggag
    23581 agttatttag taaagactca tgagattcat tataaaaaac tggccgttta tgtacctgtg
    23641 gatcatgcac agaaaatgcg tgaagtatta gctgcagctg gtgctggtac acaaggtgat
    23701 tataccggta caagttttac ctcgattgga cacggtcgat ttactccgga agctggtgca
    23761 caaccagcga ttgggaaagt agggaagaca gaacaagttc aagaggcaaa agttgaggtt
    23821 attttgccgg aaactatcga aaaacaagtg attcaagcca tgcggtcagc tcatccttat
    23881 gaggaacctg cttatgatct tttcgctata gatgaaccag tcgaaatgtt tgggttagga
    23941 cgagtaggtg aattgccgca agaaatttct atagaggcat ttgtagaaca agtaaaagaa
    24001 gcgttccaac tagatggact gagaatagtc cagccaaaaa atgcaaaatc ctctgtgaag
    24061 cgcattgcga tttgcggtgg aagcggtgaa aaattttatc cgcaagcaat cgcccaaaga
    24121 gcagatgtct atatcacggg agatatctat tatcatacgg ctcatgatat gcaaagcgct
    24181 gggttgattg caattgatcc cggtcattat attgaatcgt tatgcaagca acgatttatc
    24241 gagaaatttg aatcttggaa acaagaagaa aattgggata ttaacttttt cgtttctgaa
    24301 acaaatacaa acccatttca atttaattaa aaggagagaa tactatgtac gaaaatcttt
    24361 tgccacgttt tttacgatat gtcaaaacag aaacacgctc cgatgcaaca agcaaaacga
    24421 ctccatcgac tcagacacaa gttgcttttg cgcaagaatt gaaaaaagaa ttagaagagt
    24481 taggcttagc tgatgttttc tataatgaag ataacggatt cgttatggga acattaccaa
    24541 gcaatgttga tcatgaagtg cgttccattg gatttatttc ccacatggat acagcagatt
    24601 ttaatgcagt aggagtcaat ccacaaatca ttgaaaacta tgatggcgag tctacgatcg
    24661 tactagatgc ggaagaacgc tatacattga atacaaaaga ttttccaaat ttgaaaaatt
    24721 atgaaggcca cacattgatc acaacagatg gcagtacatt actgggagca gatgacaaat
    24781 cagggattgc ggaaatcatg actgcaatgg agatcttgat caataatcct tctatccctc
    24841 acggtaccat caaagtggct tttggtcctg atgaagaaat cggtgtcgga gcggataaat
    24901 tcgatgttga gcaattcgat gtagattttg cttatacagt cgatggtggt cctttaggtg
    24961 agttgcagta tgaaacgttt agtgctgctc aagcaaatat tacgatccaa gggaaaaatg
    25021 tacatccagg gacggcaaaa gatacaatga tcaatgctct acagttagca atcgattttc
    25081 ataatcagct tccagccgat gaagttccag aaaaaacgga aggatacgaa ggtttcttcc
    25141 atttgatggc attggatggg aatcctgaag aagcttctat gagttatatc attcgcgatc
    25201 atgatcgtga aaaattcgaa gcacgcaaag cacaaatcac acatatccaa gaagagttga
    25261 atcagcgatt tgatcaagaa cgaatcaaag tagatatgta tgatcaatat tacaacatga
    25321 aagaaatcat tgaaaaagat atgagcatcg tggaattagc gaaacaagca atgatcgagc
    25381 ttggtatctc tccagtgatc gaaccagttc gtggaggaac agatggttca aaaatctctt
    25441 acatgggtat tccgacgcca aatctatttg ccggaggaga aaatatgcac ggacgattcg
    25501 aatttgtttc tttacaagta atggaaaaag caacagacgt gattgtaaaa attgcagaat
    25561 tgaacacaaa gagttaaaat cgaaaaagaa cagcttatga caaggcgttc gtcccaatga
    25621 tggaatgtca tctgtctaag gctaaagggc aggacaatgg aattggttgt cccgccctct
    25681 tttttgaaga gtaaatagga ggtaaagaat ggaaaagctg ctagtttttt ataacaaaag
    25741 ttcagggaaa gatgagggtg agaaattagc acgctggttt caagattatg taaaagaaaa
    25801 gcagccagaa ttatatgtag aactagctga aactggccca tcgattgatg atgcggaatt
    25861 agtcaagaaa gcagaagcca tagaagctga tactttagta attataggtg gagacggcac
    25921 gattcatcat atcgtccaag cgttccaaaa aacattatct caataccatg tcggattact
    25981 tccaggtgga actgtcaaca accttgcacg tgttcttgag attcccctca aaaaagaaga
    26041 agctgctgac gtgatcctgg aaggcaatgt acggaaaata gactatggtc aagtcaatga
    26101 tgaagtcatt atctctacac tgacaatcgg aatcttagca gatacagcag tttggatcag
    26161 tcaagaagaa aaacagcgtt acggctcgtg gatctttatc aagaagtttt ttaagttatt
    26221 gctgaagaag aaaaaatatc atttggattt agaaacagac gaggagcatt ggaaaggaaa
    26281 atcccaactg ttgacagtca cgatgtctaa ctccgtgggc ggttttacaa attttgacga
    26341 ctcagcgacc ctagatgatg gaaaatttca tgtaacgatc gtaccttcct tagatatttt
    26401 ccgatttatt ttttatttgc ctagaatcat gagaggcaaa ttctatacga tccccggcat
    26461 tacttatttc acagccaaac gcatgaaaat ccaagcgcag gaaaaatctg tgggcactcg
    26521 aacggatgga gacacgacag atgatcttcc agttgaatta tctgttgtat caaaaggatt
    26581 aacagtttat tcaccaaaga aagaagaaaa agcttagaaa tagtagttaa cagaaagttg
    26641 gaagaagagt aatggatgta ttgttacaca atcctttgtg gttttacctt ttcattgtga
    26701 atttttactt attttgtctg atggggtatg ataaacatca ggctagaact agcggctggc
    26761 gtgttcctga gaataatttg atatttgtca gcctgttagg cggtggtttg ggaggattga
    26821 tctctcgaaa agttttccat cataaaactc ggaaaaagaa gtttactata ggattcgctg
    26881 caggtgtact tgttgatttg attctcattt attttttcca ttaaggagca ggagatgtcg
    26941 tctaaaggaa cgaaaactaa aatcattctg aatctgcttt tgctgattgt gatttttgcc
    27001 attatttatt atttaatcaa tcaatcattt gcagatatct ttgccgaatt gctgtctacc
    27061 agtgttgttg ttttggcagc ggctgttata ttagggacag tttaccaaat cgctgaaggg
    27121 cgctcaatca aagaaattgc tgaacctttc gcgccgaatt ttacaacgaa agatgggttc
    27181 ttgacctcta gctatattgc gttttaccga atcgtttcgt ttggtacagg aacgctgatc
    27241 tcggagattt atttttataa caaaaaagga atgaagctat cccagggagc aggggtgaca
    27301 gcactccata tgattatgta caaactggca gtgatcactt atgcattagc tggcttggtc
    27361 atccaattct ctctttttta ttctaatggt ccatcaatga tctggtttat catcgctggt
    27421 atccttttga cgatcgtgat tgttgccttt ttattggcga tgtccgtcag cttgaatgtt
    27481 caaatatttt ttgtcagctt atcgaataag ttatttaaaa gtgagcgttt gcggaaatgg
    27541 gtggatacat gcaatcagca gatctactct ttaagggaaa cggtccaaac cattatccaa
    27601 gacaggactg cgctattgcg gatttatcta tggaatatgt taaaacttgt attttggtat
    27661 gtacttcctt atttgattct gattgaaaac catcctaaaa tagattttct actgaccatt
    27721 tcgtttatca gctttgcagt agtgctttca ggggttattc ctacgcctgc tggtattggt
    27781 tcctttgaat ttgtctattt gttattattc agaccagttg ttggaacagt tgacgcagtt
    27841 tcttcgcttt tactgtatcg tttcagttcc tttatcttgc catttctcta tggatttgtg
    27901 tatgtgattg ctgaacggcg aagagtgatc aaagaagaaa taatagaagt aaaaaaagag
    27961 aataaagaaa atcaagggta ggtcttttat aagatctacc ctttttatca aatcaaagaa
    28021 aaacgttatg gataagataa ttttacgttt gtttggctct ttttttatat acatacaatc
    28081 aattttgttt ttttaagatt tatataagtc gagaaaaaaa gtcaaaaaac tatgtgtata
    28141 ttttgaccaa aattgaccaa agcgatataa tagtttttgt aaagatcaaa gaaataagtg
    28201 atcttacctt taattaggag ggtatacctg tatgaatatt gagaaaatga ctacgacgct
    28261 tcaagaagcg attgctgaag ctcaacagat tgcggtcact cgaaaacatc aagatatcga
    28321 cattgcccat gtatggaaaa tctttttaca atctaatcat tttggtcgaa atttctatac
    28381 ggatgccgga ttggatgtcg aatcttttga acatgaaatt gatcgtctac tggatgagta
    28441 tccagttgtt tctggtggaa acgtccaata tggacaaaac ctcagccaga atttctttcg
    28501 attattgaat gaagcagacc aaatacgtga aagcttcggc gacgagtttc tttcaacgga
    28561 agtcgttatt ttagcgttga tgaaattaaa aaattatccg ttaactgttt atttgaataa
    28621 aaacggatta tctgaaaaag aattgcgtaa aaacatcgaa gagatgagag gaggagaaag
    28681 agtgacctca caaaaccaag aagaacaata caaagcatta gaaaaatacg gcgttgattt
    28741 agtacagcaa gtcaaaagcg gaaaaatgga tccaatcatc ggtcgtgatg aagaaatccg
    28801 tgatgtcatc cgtattcttt cacgaaaaac aaaaaataat cctgttctga taggagaacc
    28861 cggtgtaggg aaaacggcaa tcgttgaagg gcttgctcaa cgtatcgtac gcaaagatgt
    28921 gcctgaaaat ttaaaagata aaacgatttt ttcattagat atgggtgcat tgatcgctgg
    28981 agcaaaattc cgtggtgaat ttgaagaaag actaaaagca gtcttgaaag aagtcaaaaa
    29041 aagtgatgga cgtatcctct tgttcatcga tgaaatccac aatatcgtag gggcaggaaa
    29101 aacagaagga agtatggatg ccggaaatct gttgaaacca atgcttgctc gcggtgaatt
    29161 acatttgatc ggtgccacaa ctttagatga gtaccgtcaa tatatggaaa aagacaaagc
    29221 gttggaacgt cgctttcaaa aggtattagt caaagaacca acagtggaag atacgatcag
    29281 tattctgcgt ggattaaaag aacgttttga gatccaccat ggtgttaaca tccatgacaa
    29341 cgcattagta gcagcagcta ctttatctga ccgttatatt actgatcggt tcttaccgga
    29401 caaagcaatc gatttgatcg atgaagccag tgcaacgatt cgggtagaaa tgaactctat
    29461 gccaacggaa ttagatcaag ttactagacg tttgatgcag ttggaaatcg aagaagctgc
    29521 actgaaaaaa gaatcagatg atgcaagtaa aaaacgcttg aaaaatttgc aagaagaatt
    29581 agctgaactt cgtgaagaag caaatgcgat gaagatgcag tgggaaacag aaaaagaaga
    29641 agtcaattct gtttcagcaa aacgtgctga aatcgataaa gcaaaacacg aattagaaga
    29701 tgcagaaaat aactatgatt tagaacgcgc tgctgtattg cgtcatggta cgatacctca
    29761 attagaaaaa gaattgaagg aattagaagc taaagcaaaa gacagcgaga ttaagatggt
    29821 tcaagagtcc gttaccgaaa atgagattgc tcaagtcgtt ggcagattaa cgggtatccc
    29881 cgtaacgaaa cttgtagaag gtgaacgaga aaaactaatt aaattgaatg aaacacttca
    29941 taaacgagtg attggtcaag atgaagcagt tgatgcggtc agtgacgcgg taattcgttc
    30001 acgggctgga ttgcaagacc caaatcgtcc tctaggttct ttccttttcc taggaccaac
    30061 tggtgtcgga aaaaccgaat tagcgaaagc gttagctgag aacttgtttg actcagaaga
    30121 tcatatggta cggatcgata tgagtgaata tatggaaaaa catgctgtgt ctcgtctagt
    30181 cggagctcct ccaggctatg taggttacga agaaggtggt caattaacgg aagctgtcag
    30241 acgaaatcct tatacaatcg tcttgttgga tgaaatcgaa aaagcgcatc ccgatgtctt
    30301 caatatcttg ttacaagtcc tagatgacgg acgcttgact gattcaaaag ggcgagtagt
    30361 agatttcaaa aatacggttc tgatcatgac cagcaatatc ggttcacagt tactgctgga
    30421 aggcgtgact gctgatggaa cgattccaga agcagtggct gaacaagtca acacgctatt
    30481 acgtggaaac ttcaaaccag aattcttgaa ccgaatcgac gacacgattt tatttacacc
    30541 attgagccta gacaacgtga aaggaatcgt tgacaaaatg gtggcgcaat tggcacaacg
    30601 tctggaacat caagaaatcc tgctgacaat cagtgatgaa gccaaaacgt ggattgctga
    30661 aaatgcttat gaaccagcat atggtgcaag accattgaaa cgatttatca ctaaggaagt
    30721 agaaacacca ctagctaaag aaatcgttgc aggacatgtt atgcctaaat caaaagtaac
    30781 gattacttta cttgatggac aacttcattt caaaacagaa gagctagaag aaatcgtcta
    30841 atcttccatt gagactataa aagaaaaaag gggtctgaca caactagtgt cagacctcct
    30901 tttttcaaat aaacagtgtt ccagaagtag tttcttcgaa aaataagccg aaatttcttg
    30961 aagctgcccg cttctgtcac aacctctttt attaggtatt tttcactttt tctaatttat
    31021 aactttcaaa gtaacgctca tcttttttac tttgagacca tgcaaacaat cccattaagg
    31081 cagcacctat cgtatttgtc agcaagtcgc ccatcgtatc catcaatgct gctcgtccga
    31141 caaacagtgt gccatcagaa gcagcaaaac gttgaagatt catgcctaag aactggtcgc
    31201 ataagaattc ccagaactcc caaaacacgc cgcaaagtcc agcaaacgca aagccgaata
    31261 agaggaacaa ccaaggactg gtttttgata tctcagcatc tttcatcaag taaccaatca
    31321 atccatagcc aagagctgtc aggaccattg gactcaccgc atgtaagatt ttgtcccaaa
    31381 aagagatgat actgatcaaa tgcatccctg tgcctaaaaa gacagagatg aacaagaaaa
    31441 accagtaaaa caaaacgatt gctgctggta ttttaagttt gaaaactttc aaaatgactt
    31501 gtggcatgaa aatcaatgca atccctaata aagattccac gataaatgtg attccttgag
    31561 aagtcgaata tttttgtgtg ataaattcat aaaaattata gcagacaacg aatacaccaa
    31621 aagcaagtaa agctatttgc acctttttga tttctttaat cactgaaagt ccctccatct
    31681 aaaaatcttt ttacttctga aagcaactca atttctgaag aaaccacttt tccgttcaat
    31741 ttgatcaaac ctattgtata taagttcagg tagtgaaatt gattttcagc tgtttcttgt
    31801 agtgcagcga tttttttcgg gtgggttccg ccttgctgtc gagtatcagt atataagccg
    31861 atcactggga tgcctttcgc ataagccaca ccgatttccg atgctacccc ggcatcgatc
    31921 gtcagcccat ctagtaaagc aatcattaaa tcactagcca acactttttc tgtgtcagca
    31981 agagcaatca ttcgactgtc tgcataagca gttttgtcat tgattgcttc attttcttga
    32041 ggaagataga tcgaaagctc agaagagagc tctctgattt ctttaactaa ctgttggtta
    32101 tataacaaat cacttttagc aaacattggt gcggcaaaat aaattttcat ctaaacatca
    32161 tcctttttat caaaaataat cgctaaagta agcttccatg tttggaatga tacgctctag
    32221 tgacttcagt gtttcttcat ctgtttctaa ccgttcgatt cttgccaaat aagaagcacg
    32281 gcgataattc atcatcaagc aagtcaatgt ttggatatcc agtcgaacgg attttcctat
    32341 aggttcattt aagacacgga cttgtccctg ttcatcccat gttaaagcga agacaccatt
    32401 attccaagga gcgacaggat cttcaatgat gaaatgaaaa gcatcagctg ttccaacaaa
    32461 cggaaaccgt tgaaggaatt cttttacatc aacgatccga gccataaaat atggttcgat
    32521 ctgctctttg atctgactat cttcaatcaa aaatgctaaa ggctcatttt tataaatatc
    32581 tccatgtacc cagtagatca tggaaaaatg tgcggaaata aagttccata agccatttcg
    32641 agcttcttga tcaagataga acatttcttt tacgtgaaat atttcttcag ctacccagta
    32701 gaacagaaca cctcttggtt catggttgct atcgtagtag acagcggctg tgcgctcatc
    32761 ttcattttcg aaacgccaat actcttccca atgaaaatca ctgcggaata aagcaccatg
    32821 gttttgttgg gcaaatttgt cataaacgga aaatacgtct ggatgatcaa ctggtaatcg
    32881 ctcaaccata ccacttactg ggatttgttt aggtagctgt gtatctctga ttttaaagga
    32941 aagtttatca gacatgattt cccatccttt acgccgatag taggggatac tgtaaggata
    33001 caaataagag atccattgct tattcttgcg catattatcc aatgcaacga taattagatc
    33061 ttgcatcaat ccatggttag catattctgg ataagttcca actccagtca caccacccat
    33121 tttataacgt gcaccgtgaa tattgacttc acaaggatag atagcgattt gagagatcag
    33181 tttattttcg tggaaccatc cgaacacttt cgatacttcc aaaatcggtt ttttggaacg
    33241 gataaaagct cgtttattct caaacccgct ttcctcgatg tctgcttcag tcacttggaa
    33301 cacataggaa agcaattcat tgaattgatc gatatgttct tgagtgaccg gttttaattc
    33361 aagattttct ctaaaatctt tttgatccat tttttcatct ccatttatta gcttgataac
    33421 agtatagcaa aattctacaa aaaaaacttc tatagctgac taattaaaaa ctctttaaat
    33481 tactgctttt cgttggtata atggaaagtg ctgattatat gaaaagagga gacgctatgg
    33541 atataaagaa aatgaaagaa cgccaagagc agattcgtaa tttttcgatc atcgctcata
    33601 tcgaccatgg gaaatctaca ttggccgatc gtattttaga aatgacgcac actgtgacgt
    33661 ctcgcgaaat gcaagatcag ctgttagact cgatggattt ggaacgtgaa cgtggaatca
    33721 cgattaaatt aaatgctgta gaactcaatt acacagcgaa aaatggagaa acatatatct
    33781 ttcacttgat tgacacccca gggcacgtcg acttcaccta tgaagtctct cgaagccttg
    33841 ctgcctgtga gggtgccgtt ttagtggtcg atgcagcaca aggaatcgaa gcacaaacat
    33901 tggccaatgt ttatttagcg ttagacaatg atttggagat cctgccagtc atcaataaaa
    33961 tcgacctacc cgcagcagat cccgaacgtg tccgtcaaga aatcgaagac gtaatcggaa
    34021 tcgatgctag tgaagctgta ttagccagtg ccaaatcagg gattgggatt gaagatattc
    34081 tcgagcagat cgtggaatat gttcctgcac caagtggaga cttagatgca ccgctaaaag
    34141 ctttgatctt tgactctatt tatgatagtt accgcggtgt tgttctaaat gtccggatca
    34201 cggacggtat ggtcaaaccc ggagacaaaa tcaaactgat gagtaatggc aaaacatttg
    34261 atgttacaga ggtcggggtc ttctcgccaa aagcagttgc tagagacttt ttgatggtcg
    34321 gtgatgtcgg ttatatcaca gcaagtatca aaactgtcca agatacacga gtaggggata
    34381 cagtaacgtt agcagacaat cctgccgctg aagctttacc aggttaccga aaaatgaatc
    34441 cgatggttta ttgtggtctt tatccaattg atacttctcg ttacaacgat ttgcgggaag
    34501 ctttagaaaa actgcaattg aatgatgctg ctttacaatt cgaaccagaa acctctcaag
    34561 cattaggttt tgggttccgc tgtggattcc tcggacttct tcatatggat gtcgtccaag
    34621 aacgcttgga acgggaattc aatctagaat tgatcacgac tgcaccatcc gttatttacc
    34681 atgttaataa aacggatggt acgactgcta cagtagataa cccggcagat tttcctgaac
    34741 ctgtaacgat tcaggatgta gaagaaccat tcgtcaaagc acagatcatg gtcccaaatg
    34801 attttgtcgg tgcagtaatg gaactttctc aacgaaaacg gggagaattc attacgatgg
    34861 attatttaga tgattaccga gtaaatgtcg tctataatat tccgctttct gaaattgtct
    34921 ttgacttttt cgacaaattg aaatcaagca cgaaaggcta tgcctcattg gattatgaaa
    34981 tgtctggtta tcaaaaaagc aagttagtga agatggacat cttgctgaat ggagaaaaag
    35041 tcgatgcatt aagctttatc gttcaccgag actttgccta tgaacgtgga aaagcaatcg
    35101 ttgaaaaact gaaaaaacta atcccacgcc aacaatttga ggtgccaatc caagcagcta
    35161 ttggacaaaa aatcgttgcc cgttcagaca tcaaagcctt gcgtaaaaac gtcttggcta
    35221 aatgttatgg tggagatgtt tctcgtaaac gtaaactctt agagaaacaa aaagaaggga
    35281 agaagcggat gaaacaaatc ggctccgtcg aagttccgca agaagccttc atggccgttc
    35341 tgaaaatgga cgaggacgaa ccgaagaaat agcaagggtt tgtttgaaaa cagtgtaaaa
    35401 aataaaccat gtttacatgg atttgccacc ggtttgccac cggtggtttt tttcggtggc
    35461 aaaaatcaag aattctttct aagattttcc atgatatcga ccgtttcatt tctcatctta
    35521 tctgttacat gcgagtaagt atccatcgta attgaaattc tgctgtggcc caatcgttca
    35581 gagatttctt tcatttttgc gccattttcg agaagaagtg tagcatgagt atgtctgaga
    35641 gaatggaagt taaaagagag ggagagtgca ttcgatatcc tcctagtatt ccatttcact
    35701 acacttggcg taactagctc accgtcttcc ttcgtacata ctgcatttga atcaatgtag
    35761 agctttccat acttcattcg attttctaat tgttgtttct tatgtttttt cagaattgct
    35821 agcaaggttt gtccaataaa aatcgttcga ttggaactgc ttgtctttgg cgtaccatat
    35881 acccatgcgc cgtcattctt taccatttgt ttctctacag taattgttcc atttgaaaaa
    35941 tcgacatcat cccacgtcag accacaaact tcgccaacgc gcattcccgt ataaaatcca
    36001 atattcaaag gaatatagaa aggatgtcct tcaggagtaa tttctagcat atgatcaaag
    36061 tcctcaagag aaatgatttt tagatctttt ttagtcgttg gtcgtgcttc atattttggt
    36121 atttttacat acagcatagg attttgcttg attaatcccc aaggataaac cgccatattc
    36181 agcgcattct taaggacaga gtgagtaata gtcattgttt tcttcgagta acccttttta
    36241 aattcagcat tgatgaaatt ttgtaaaaga gcaggggaga gatccgtaag ttttttcttt
    36301 cctaaataac cgtttatatg atttttgatg gtaaatcggt agttttcata ggtattgtat
    36361 tttagattta gtttaacgta ttcctccatc caaaaatcaa ggtattgttt tactcgagta
    36421 tccgtaccta aaaagtattg tcctgtttcg tcaatatctg ataaaacttt tcgtaaagca
    36481 gcttcggcct ctggtcgggt gtctccgcca actttctcca ctttttttct tgagccatca
    36541 tcattgatat ct
//
LOCUS       DAMYXM010000022        34998 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.023, whole genome shotgun sequence.
ACCESSION   DAMYXM010000022 DAMYXM010000000
VERSION     DAMYXM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.023"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            101..1246
                     /locus_tag="PNS21_001268"
     CDS             101..1246
                     /locus_tag="PNS21_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDI5158985.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1383..1727
                     /locus_tag="PNS21_001269"
     CDS             1383..1727
                     /locus_tag="PNS21_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HDI5158986.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            1998..3284
                     /gene="efmA"
                     /locus_tag="PNS21_001270"
     CDS             1998..3284
                     /gene="efmA"
                     /locus_tag="PNS21_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HDI5158987.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3422..4021)
                     /locus_tag="PNS21_001271"
     CDS             complement(3422..4021)
                     /locus_tag="PNS21_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HDI5158988.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4425..5546
                     /gene="mnmA"
                     /locus_tag="PNS21_001272"
     CDS             4425..5546
                     /gene="mnmA"
                     /locus_tag="PNS21_001272"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDI5158989.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5792..7213
                     /gene="arcD"
                     /locus_tag="PNS21_001273"
     CDS             5792..7213
                     /gene="arcD"
                     /locus_tag="PNS21_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDI5158990.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7227..8345
                     /locus_tag="PNS21_001274"
     CDS             7227..8345
                     /locus_tag="PNS21_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HDI5158991.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8405..9697)
                     /locus_tag="PNS21_001275"
     CDS             complement(8405..9697)
                     /locus_tag="PNS21_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDI5158992.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9829..10407
                     /locus_tag="PNS21_001276"
     CDS             9829..10407
                     /locus_tag="PNS21_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI5158993.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10536..10913
                     /locus_tag="PNS21_001277"
     CDS             10536..10913
                     /locus_tag="PNS21_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDI5158994.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10846..12918
                     /locus_tag="PNS21_001278"
     CDS             10846..12918
                     /locus_tag="PNS21_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDI5158995.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(13129..14076)
                     /locus_tag="PNS21_001279"
     CDS             complement(13129..14076)
                     /locus_tag="PNS21_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDI5158996.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            14322..14786
                     /locus_tag="PNS21_001280"
     CDS             14322..14786
                     /locus_tag="PNS21_001280"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDI5158997.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(14848..15891)
                     /locus_tag="PNS21_001281"
     CDS             complement(14848..15891)
                     /locus_tag="PNS21_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDI5158998.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(16268..17233)
                     /locus_tag="PNS21_001282"
     CDS             complement(16268..17233)
                     /locus_tag="PNS21_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDI5158999.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            17467..18312
                     /locus_tag="PNS21_001283"
     CDS             17467..18312
                     /locus_tag="PNS21_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI5159000.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            18503..19690
                     /locus_tag="PNS21_001284"
     CDS             18503..19690
                     /locus_tag="PNS21_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDI5159001.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            19696..20052
                     /locus_tag="PNS21_001285"
     CDS             19696..20052
                     /locus_tag="PNS21_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDI5159002.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            20054..20392
                     /locus_tag="PNS21_001286"
     CDS             20054..20392
                     /locus_tag="PNS21_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDI5159003.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    20468..20724
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            20787..21821
                     /locus_tag="PNS21_001287"
     CDS             20787..21821
                     /locus_tag="PNS21_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI5159004.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            21814..22500
                     /locus_tag="PNS21_001288"
     CDS             21814..22500
                     /locus_tag="PNS21_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5159005.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            22524..23372
                     /locus_tag="PNS21_001289"
     CDS             22524..23372
                     /locus_tag="PNS21_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDI5159006.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            23569..24342
                     /gene="sufC"
                     /locus_tag="PNS21_001290"
     CDS             23569..24342
                     /gene="sufC"
                     /locus_tag="PNS21_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDI5159007.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            24355..25641
                     /gene="sufD"
                     /locus_tag="PNS21_001291"
     CDS             24355..25641
                     /gene="sufD"
                     /locus_tag="PNS21_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDI5159008.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            25638..26873
                     /locus_tag="PNS21_001292"
     CDS             25638..26873
                     /locus_tag="PNS21_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDI5159009.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            26860..27330
                     /locus_tag="PNS21_001293"
     CDS             26860..27330
                     /locus_tag="PNS21_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDI5159010.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            27335..28726
                     /gene="sufB"
                     /locus_tag="PNS21_001294"
     CDS             27335..28726
                     /gene="sufB"
                     /locus_tag="PNS21_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDI5159011.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(28821..29963)
                     /locus_tag="PNS21_001295"
     CDS             complement(28821..29963)
                     /locus_tag="PNS21_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI5159012.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(30002..30736)
                     /locus_tag="PNS21_001296"
     CDS             complement(30002..30736)
                     /locus_tag="PNS21_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI5159013.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            30969..31244
                     /locus_tag="PNS21_001297"
     CDS             30969..31244
                     /locus_tag="PNS21_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5159014.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            31342..33339
                     /locus_tag="PNS21_001298"
     CDS             31342..33339
                     /locus_tag="PNS21_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159015.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(33415..33750)
                     /locus_tag="PNS21_001299"
     CDS             complement(33415..33750)
                     /locus_tag="PNS21_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI5159016.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            33877..34092
                     /locus_tag="PNS21_001300"
     CDS             33877..34092
                     /locus_tag="PNS21_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI5159017.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            34129..34491
                     /locus_tag="PNS21_001301"
     CDS             34129..34491
                     /locus_tag="PNS21_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDI5159018.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            34537..34863
                     /locus_tag="PNS21_001302"
     CDS             34537..34863
                     /locus_tag="PNS21_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159019.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT        11530 a         6203 c         7329 g         9936 t
ORIGIN      
        1 tttttctatg cccatgaaac gtcctctaga caaaaatgag ctcttctttg gtttgttcgc
       61 tattttatgc tagaatgtgc ctagcaataa tttggaaggg atgaatgaag tgaaaactat
      121 ttatctggat catgcggcaa ctacccccat gcatccacaa gtaattgaag cgatgacggc
      181 aatcatgcaa gacacgtttg gaaatccttc aagcatacat ggatttggtc gccacgcgca
      241 tgaaaaattg gaaacagcaa gacaaatcat tgcagacagt ctgcaagcaa aacctcacga
      301 gatcatcttt aacagtgggg gaacagaagg ggataataca gctatcttag aaacagcgtt
      361 ctcgcgtcaa aaagaaggca gacatctaat cactacagct atcgaacatc ctgctgttct
      421 taataccatg aaatatttag agacaaaggg gtttgacgtt acatatctgc ctgtagatga
      481 aaaagggaat ctgtcgatcg aagaggtcaa aaaagcgtta cgagaagata cgatcttggt
      541 ctctgtgatg tacgggaaca atgaaatcgg aaatttgatg ccaatcaaag aaatcggtga
      601 acttttagcg gatcatccag cttatttcca tacagatgcg gtacaagcgt acggaaacca
      661 aacgatccat ccagaagaat taggaatcga tttattaagt atctctgctc ataaaatcaa
      721 tgggccaaaa ggcgtcggtt tcttatataa acgagacgga gtaaatatcc ctgcattgct
      781 tttaggcggg gagcaggaag aaaagcggcg tgcaggtaca gaaaatttag cagggatctg
      841 gggtatggca aaagcagttg aactgctgtc acctgaagaa cgtgaagcac gtaacaacaa
      901 atataaagga ttccagcaat tgattcttga tacattagaa gcaaacgaaa tcccttatca
      961 agtaaatggt gatttaacga ataaactccc tcatgtgttg aatcttcgct taattggcga
     1021 agcaaatgat ttgctactga tgaagcttga tcttaatggg gtcgctattt ctactggttc
     1081 agcttgtaca gcaggaaata tcgagccttc acacgtatta gaagcaatgt atggagaaga
     1141 tacaccgata ctaaaagaat ccatccgtat cagctttggt ttgggaaata ccgaagacga
     1201 catccagtat ttccttgatc aattagttcg tgctgtcaaa aaataactag tgaagtcatt
     1261 cttatatgaa cagatgtctg agagaacgcc ttcatttttt agggaactta actgaaaaac
     1321 ctagtaaaaa tcaggataaa cttttataat aaagagaaac taattttatc gaggtgaaaa
     1381 tcatggcatt tgaaacaaca gcaacggtac tgggcgcacc aattagctat cgtttgcatc
     1441 ctgatgcaaa aagatataca ttgcgcgata atggatttac agaaacaaac ggaggcaatt
     1501 atcaattgat ccgaccatta gatgccacgc cgcaaagcaa agaaggattc aaattaaaaa
     1561 tcactgtatc aaaagatatt caaacattga aaatgtcgat cactacagca aatggattga
     1621 aagcagtcaa tattttcaaa gatccaaaac agaaaatgag ccaagataaa ttttattttc
     1681 tgatggatgg gatgattagc cgtgggttat tcgaaaaagt cgaatgatct gtggatataa
     1741 aaagacaact agttttgaaa cggcctattg ttagatgact aactttgtaa ggacgacgca
     1801 aattagttgt cttttttatt tttaaattta acacatgttg ttatttttaa tattttgctg
     1861 atttattgtt tctaagtaaa gaaatttaaa ttgaaacgat tgttatttag ggaaaaaggt
     1921 attttttgaa aaaagaatcc cgtataatga ttgttgtgca aacgctatac aaaatgaaaa
     1981 agaaaagagg gatgtttatg gaaaatgaac agtcggtcgt tttaacgaat tggaagagaa
     2041 attatttgtt ctttttatcg ggacagtttt tgtcaggaat cactagtatg gtcgtccaat
     2101 atgcgatcat ctggtatttg acaagagaaa ccggttcggc gacaattttg agttttgcta
     2161 ctttactagg aatgattccc atggttttac taagcccatt tgtaggaccg ctagtggatc
     2221 gctgggataa aaaagctctt ttgattgtca cggatattat tgtagcgatt ttcgcgctta
     2281 ttttagcagt agtcggaacg atttcagaat ccttcccgat ttggctagtt tttgtgtctt
     2341 tgtttatgcg ttcggtcgca caaacattcc aaatgccaac gatccagtcg attatgccga
     2401 caatcgttcc atcttctcac ataacaagga cgaatggaca gttaggaatg gtccaatcag
     2461 cgaatttcat cattgcgcca gctctaggtg cggctttgtt ttcagtcgtt cctgtcaatt
     2521 accttatttt attagatgta ttgggggcag tttttggtgt cggcttattg atttttgtga
     2581 agatcccaaa agtttctcct gaaatattgg aagttccgct tactattttt aaagatgcaa
     2641 aatttgggct gcagcaattg atggataata aagggttatg gtacataacg atcaatggtg
     2701 catttgtgat gctgttgttc atgccagcaa tcagtctgta tccattgatg acgctagatt
     2761 actttggtgg ttccgttgga caggcgggag cagtggaagt agtctacgca gtcggaatgc
     2821 tcttaggcgg tgcactgatc agttttatag gaacatggaa agaccgaatg aaacctatta
     2881 ttatagccta catcattatg ggcctgacga tcggtgcaag cggattggtt ccaaacgata
     2941 gtcaagggtt cttgtacttt cttatcttga atgcaggagc aggttgtgca acgccatatt
     3001 tcaatacact gctgatggca atgatccagc agagttatga atcgaatgtt ttaggtcgtg
     3061 tcttagggaa cttcaactct ttgatgaacc tggcaggtcc aattggatta ttatttgcag
     3121 gaccattagc tgatcgttta ggggtagaaa aaatgttctt gttttccgga atcgggattt
     3181 tattatgcgg aatcgtttta tttttaacgt cagctgcccg aaattatgat aaagaattac
     3241 agaaaaaact ggtaaaagaa caccatgagc aaaaagacga atagtatgga atggagttgt
     3301 gacagaagtc ttgttacaac tccatttctg atcctaatgt tcctgtggca ataagcctac
     3361 tcaaatgaaa aatatgagta attatcttta tttgtttaat acttgatata gggcagtttt
     3421 ttcacgacct atcgtacttc ctctttgctt catagatttc tttgatttgt tgctcagata
     3481 acggagtgcc aaatggtagt ttttccttta agagttctct ttccggtgcg tctacgccta
     3541 cattgacatc gttctcgata ggtacgctgt aatgatggat atgtccatga agattgcgga
     3601 tagtgtcgtt aggcccgagc aacatcggat aatgagtcaa gtaatattgt tgatggtcga
     3661 atttaacgat ggcacctaca tcataaaaat aaaatttggg acttccgtca gtaagcctag
     3721 gatcattttt ttctaaataa cggaaaaaag cgcgactgtc atggttccct ttgatcaagt
     3781 ggattgttcc ttgcagttga gtgaccagtt ccaaaatttc tgcatttgct ttttcatatt
     3841 gcggatgcat agcaaggtcg cctagatgat aaacatggtc ggtttctttt attactgcat
     3901 tccagctagt gatcaactgt tggttcattt catccacatt tgcaaagagc cgcggagcaa
     3961 agtcgttttt tcctaataag cgttcatgga aaaagtgcat atcactgata aagtaattca
     4021 tgtatctttt gcctcccttt tttcttagag taaacaaaat cacttatctg agcaactcat
     4081 aacatctaaa aagaaaaggt tgtaaatgag acgtgcagct ttaagcgata agaagcaatt
     4141 ttgaaaaata gtttttcaca atttataaaa ttattcctta ttgccgaaag gctggccctt
     4201 tttatgagta tgccaatttt ttatatcgaa tgctcaaaag atgagtgatt ttttggttga
     4261 ctatcatgtt tgtgtactga ataggtccag tagaacagta tttttacgac ttgataatta
     4321 agggatgaaa ttttcagaaa tatgacgtat aatgtaaaat actgaaaagt tgcgaccttc
     4381 aactcgtaga tgtttcagaa tctaattgaa atgatggtga tatcatgaca gacaacagca
     4441 aaacacgcgt tgtcgtaggg atgagcggcg gtgtcgactc atctgtaaca gcacttttgt
     4501 taaaggaaca aggatacgac gtgatcggaa tattcatgaa aaattgggat gacacggacg
     4561 aaaacggtgt atgcacggcc acggaagatt ataaggatgt agcgaaagta gctgctcaaa
     4621 tcgggattcc ttattactct gtgaattttg aaaaagaata ttgggatcgt gtatttgaat
     4681 atttcctagc cgaataccgc gcaggacgta cacctaaccc agacgtgatg tgcaacaaag
     4741 aaatcaaatt caaagcattc ttggattatg caatggatct tggcgccgat tatgtagcta
     4801 ctggacatta tgcgcaagtc actcgcgatg aaaacggcgt ggtccacatg cttcgtggag
     4861 tagacaataa taaagatcaa acttacttcc ttagccaatt atcacaagaa cagctttcaa
     4921 aaacgatgtt ccctctaggc ggcatggaaa aatcagaagt acgggcaatt gctgaacgcg
     4981 caggccttgc gactgctaag aaaaaagatt caacgggaat ctgctttatt ggtgaaaaga
     5041 acttcaaaca atttttgagc aactatctgc cagcaaaaaa aggaaatatg gtcactcttg
     5101 atggtgaagt caaaggacaa catgctggct tgatgtatta tacgattggt caacgtcaag
     5161 gactagggat cggtggcgga ggagattcgc aagaaccctg gtttgttgta ggaaaagatc
     5221 tagcgacaaa cacattatat gtggggcaag ggttccatca tcctgcattg tatgccacaa
     5281 gcttggatgc aagtgaaatc cactttacga cgaatgaacc aatgccaaaa gaattcaagt
     5341 gtacagcgaa attccgttat cgtcagcaag acgttcctgt gacagttcgc ttattggatg
     5401 ataatcgtgc agaagtcgta ttcgacgaac ctgtacgtgc aatcactcct ggacaagcag
     5461 ttgtatttta tgatggaatg gaatgtctgg gtggtggttt gatcgaccac gcatatcaag
     5521 aaacaaaagt tttacaatac gtttaagcga tattaaatga atcaaaacag ctatggctaa
     5581 caaaagaaac accttttctt ttggatagca tagctgtttt tttgcaataa aattaatttt
     5641 tattcatttt atacttgaaa tacttgtgta aaaagttcat aatatccaat aatatgaata
     5701 aagttgcttt ttgttaagca agagcagttg caaaatagaa gggaatcttt tgcaagtgat
     5761 ttgttcaatt ttataagtga gggtgatgga aatggaaaag aaatcgacgg gtattagcaa
     5821 atggggactg gttgctctgg ttgtcagttc gtcaatcgga agcggtgtgt ttgggatcac
     5881 aagtgatctg gctagttctg cagctccagg gccagcgatc ctttcatgga taatcgttgg
     5941 gattggtatt ttagctttgg tattatcgct gaatcattta ggagaaaagc gtccagatct
     6001 agatggtggt attttcggtt acgcggaagc ttcatttggt aaattaggtg gcttcatcag
     6061 tggttggggt tactggttgt ctgcgtggct gggaaatgtt gcttttgcaa cgatgctgat
     6121 gagcgccatg ggagaatttt tcccgatatt caaaggtgga caaaatgttc cttctatttt
     6181 atttgcaagt atttttattt ggggtctgac tttattagta aacaatggag ttgaaggagc
     6241 gagcgtcatc aatacaatcg tgacgatctg taaattagtg ccattattcc tatttttaat
     6301 atttgtattt atcgcattta aagtgaatat attcactgct gatttttggg gtaacgtatc
     6361 tgataatcta gtgaatggaa cagggcagca aggaacgatc tggttgcaga ttaaaggttg
     6421 tttgatggtt atgatgtggg tctttgtagg gatcgaaggc gcaagtgttt tagccaaccg
     6481 cgcgaagaaa cgttcagaag cccagcaagc atcgatcatt ggtctgcttt gtttattgtt
     6541 tatctatatc cttgcttcga tcttgccata cggcgtctta tctcaagaag agttagcaac
     6601 tatttcacaa cctgctatgg caaatatttt aaaaggaatc gtgggaaatt ggggagcagc
     6661 gctgatcaat atcggcttga ttatttcgat cattggttct tggttgtctt ggacgatgct
     6721 cccagcagaa acaacgatgt tgatggctcg agacggtctg ttgccgaaag gttggggaaa
     6781 aacgaataaa aaaaatgccc caacttattc gttagttgtt actgcaatct tgactaatct
     6841 attcttattg actttcttag ttactgatta tgcctatcaa tttgcctatt ctttatgtac
     6901 agcggcaatt ttgatttgtt atctacttgt cgggatctat caagtccagt tttctttaaa
     6961 agaaggaatc aaaaaacaag tagtgatcgg tgggattgct gtagtttttg aattgatggg
     7021 catcatttta gcaggatttt cttatgtcct tttatgtagt attgcctatc ttcctggttt
     7081 ttgcttctac tggatcgctt gtcgtgaaac caaacatcaa attacgggaa aagaaaaagt
     7141 aatgattggg ctgatttgtg tggccgcact gctttcaatc gggttgcttg ctgtaggctg
     7201 gatcaagata taagggggaa agaaacatga aaatcgcaaa tttcggtgta gaagaatggc
     7261 taaataaatg ggaaaagaaa gcggtatatg atattgcaca aagctcgatc gaagcattga
     7321 cattagacga actcgttggc ttagatggga ctagcgtaag taaatttttc gaaaaacgca
     7381 aaaacgaacc attggactat ggctggatcg aaggatcaga tgttttcaaa caagaagtcg
     7441 cttctcttta tcaaaccgtc gatccggaaa acatcttaca aacgaatgga gcgactggtg
     7501 ctaatctttt agctctatac gcgttagtgg aagcaggaga ccatgttgtt tcgatgcttc
     7561 ctacttatca gcaattatat gacatcccca aatctttagg tgccgctgtt gactttgttc
     7621 atttgaagga agaagaagat tggcagtttg atttagaaca gctagaagcc ttagtcaaac
     7681 ctgaaacaaa aatcatctgt ttgaatagtg caaacaatcc tacaggcaca ttgctagatc
     7741 ggaaaacttt agaaaaaatc gcagaaattg ctcgtacagt agatgcatat gtattgatag
     7801 atgaagtata tgcgccttta acggataaag gtgaattctt gtcgatcgta gatgtctatg
     7861 aaaaaggtat agcaaccaat tccctatcca aaacctactc cattccaggt attcgtattg
     7921 gctggacagc tactggacct gagttggcag aagtttttcg aaagtatcga gactacacga
     7981 tgatttgcgg cggagtcctg tcagatgatc ttgctgtaca tgcattgaag aataaagaaa
     8041 aaatattggc gcgaaacaaa aagatcatta cagagaacct tgtgatttta aaacaatggg
     8101 tagccaatga accgaaagtt gaacttgtcg ctcccaatta cgtctctact tctttcatta
     8161 aattagccat cgaagaagat gaccagacat tttgtatcaa tttattagaa gaaacaggag
     8221 tactattagt accaggaagt gcctttgatt taccgaagca tgcccgacta ggttactgtt
     8281 gtaaaaaaga aaccttggaa aagggattat tcctcttatc tgagttcttg aaaaaacagg
     8341 cctaaacaaa aacactgcaa ggacagtctg tgacaactgt tcaagcagtg tttttcattt
     8401 acgcttattg tctatgagaa ggagagtgcc ctgaagctat ggagactcct gaagcttttt
     8461 cttctgcagt ttcttgctga tggatctctt gtgtattgtg tcgtacaagc aagaagttga
     8521 ccaaaacaag aagagaagtc gaaagaaaga cgccacgata accaaatgca ctggaaacac
     8581 tggagccgat cataggccct actacgttac cagttgcttg gaaagattgg ttataactga
     8641 agatccgtcc agcagcttga tgaggcgaat attttgtgat caatgcttgg acagcaggga
     8701 gcagacaagc atcagagata ccaatcaaaa aacgtaaggc agctaattgc caaacattcg
     8761 tgacgaatgc cattgggata taaaccagaa tcgcaaaacc caaaccaatc gcaagtattc
     8821 tttcactccc aatcttgtca cctaagcgac caaatcttgg agcggctatc aaagtggcaa
     8881 tcccaggtat cgaagcaatg attccgctga tcaacgtaac attcccatgt ccatgcagca
     8941 attggcgaat gtacagactg atgattggac taatggaatt gttggatgct tggatgatca
     9001 tcgttgtgac gaacataccg atgacgacgt gtggatattt caagtctttg aaaatctgtt
     9061 tggcagaaac catgttttct tttttcactg ggacaaattt ttcatggacg aaaaacagac
     9121 tcaacaagaa gacgaaaaat aagatgatcc ctgtaatgaa aaaggtaggg cgatagccaa
     9181 acgctgaagc agagatcccg ccaagaagcg gtccaagtaa tgttcctgta acagaaccag
     9241 ttgctaaggt tccaagaact tgaccacttt tttcttttgg cgtcccagtt gcgacaaggg
     9301 cagtggcatt actgatataa ccagaaaaaa tcccttgaag cagacgcaat gcgactaatt
     9361 gatacacact agtcacgcaa cccatcaaag aaatgacgat tgccattcct aatgaagcac
     9421 gcaacagcat caattttctt cctttttggt cggctaattt tccccaccaa ggagagataa
     9481 gcgtagtcac caagaaagta gatgaaaaag tcaatccgct ccaaaagttc aattgtgacg
     9541 tactataatt acctaacgta tctatgtaaa gtgacataaa aggcatgact aaactaaatc
     9601 cgataccagc cataaatgtt ccaaaccata agacaaacaa gttttgttcc caagtttctc
     9661 ttttcctaaa taatttttcc tttattcttg cagccaatta gactccttct ttctgaaaat
     9721 tttcatctac agctctttat tataaactac aatatgtagt ttatcaatga aagtctcttt
     9781 tttctctttc tttttcgtgg taaactgaaa gagtgaagga tttattttat gagaggaaac
     9841 atggaaatga aaaaaagaaa tctgtctcaa gagaaaatca tcgactgctt tcgagaacta
     9901 gcagaagaga tggatgttca gcaaataacc tttcagcatt tagccaaacg cttggatatc
     9961 aaggctccct cgctgtataa ccatttcaaa aatattcgtg atgtcaaaac agcgctaaca
    10021 gcgaaacttc tgcaggaact taatgaccag ctgcgacggg ctcttgtggg aaaaagtggt
    10081 caggaagcac tgcagatcta tgcagaaact tatcgatcct ttgctttttc aaatcaagca
    10141 gtgtatgagt tactgatcag tgtaccgcat accaatgaag ctgttttatt agatggaatc
    10201 catgaaacga atcagatcat tttgcagata tttgaggctt ttcaattaac gaaagaagaa
    10261 aaagttcatc gaagcaggga acttcgtagt atgatccacg gttatctttc cttacgtttt
    10321 ttaggatact ttacaaaaga accaacaatc gatccagaag acagctattt ttggatgatc
    10381 aataacttta tcgctacttt gccttaaaca atcagattca tttgtaacaa ttttaagatg
    10441 ttcattaaat gaccctttta taagagaaaa ttcttgcaac tttcttgaaa agtgactaag
    10501 atgagtctag aaaagctgaa agaaggagca cgagtatgta tcaagtaatt accatgttcg
    10561 gggacaatga gccttggtgg ttttttgaag actgggaaga agatattgaa gaagaagaaa
    10621 cattcgagtc tttcgaagaa gcacgacaag cgtacgaaaa acaatggaac gaaatccatc
    10681 aaaattatga atatatcaat gcaaaaagta actttctcag cgccttttgg aatgacggag
    10741 atgagcgctg gtgtgaagag tgtgacgatg atttacagca atacaaggga cttgcgttgt
    10801 tgaaaaatca tcagcccatc acagtagaaa gcagaaagga attttatgaa acaactaatt
    10861 ctagcggaaa aaccaagcgt tgcgaaagac ctaagcaagg tgcttggtgc taatcaaaag
    10921 cacaaaaatt attatgaagg acccaaggtg atcgttactt gggcccttgg tcatttatta
    10981 ggattgaaaa tgccggaaga tctgaataag gaatggcaga gctggcaaat ggacacgttg
    11041 ccaatgatcc ctaaaaaact aggaatcaaa ccacttccta aaacaggaca tcaattaaaa
    11101 gcaatcaagc agctggctca gcgaaaagat gtctcagaag cagtcattgc gacagatgcc
    11161 ggaagagaag gggaacttgt tgctcgctgg atcttagagt atgtgcattt caataaacca
    11221 gtgaagcggt tatggatctc ttctcaaaca gataaagcaa tcaaaactgg attcaagcaa
    11281 ctaagacctg cgaaagaata tgatgcattg tatgaatctg ctcttgcccg ggcaaaagcg
    11341 gactggctgg taggattgaa tgtgaccaga gccttaactg tgaagtacgc ggataattta
    11401 tcagcaggcc gtgtgcagac accgacacta gcattagtga gagaccaaga aaagaaaatc
    11461 gagcagttcc gtccacaaac gtatttcacg atcacgttgc aagctggaaa agaacaagcc
    11521 aaaatgcttc aaaaaaatca atttgcttta aaaacacaag aagaagcaca aaaacttgtt
    11581 caattgatca gtacaagcaa aggaactgtc acagaagtcc aagaaaaatt aaaaacggaa
    11641 tctgcgccat tgccttatga tttaacagag atccaaagag aagccaatca acgctatggc
    11701 tattctgcaa agaaaacatt aagtcttgta caaagtttgt atgagaccca taagatcgtt
    11761 acttatccgc gtacagacag caaatatctg acaaatgaca tgaaaagtac gatgaaagaa
    11821 cgattgcaag cagtcagcga ctttgctcct gaggtcaagc agtatttgaa aaacggagcc
    11881 atcgtccaac agacaaaagt attccaagac agtaaagtga ccgatcacca tgcacttttg
    11941 ccaactgaaa acagagcacg ttatgaaaaa ttaagtaatg aagagcgaaa aatctatcaa
    12001 atgattgtct ctcgtttttt aggactgttt gctcaacccc ataaagtcag ccaaacaaaa
    12061 gttacggttg aatttggtaa agaacgattc atttttcggc aaaatcgtgt cgtccaagca
    12121 ggatggaaag gcgaaactga aactgagacg gaaacagtca aatgggaaaa aggaatgaga
    12181 atcaatcccg atttcgccat caaaaaagaa ctatctgcac cgcctaaacc attgtcagaa
    12241 gcaagcttgc ttggtcaaat ggaaaaatac agtctgggaa ccccagctac gcgtgcagag
    12301 attatcgaga aactaatcaa atctgaactg atggaacgta cccctcaagg attgcaagta
    12361 tctcctaaag gtaagcaatt attagtattg gtaaatccct ctttagtaac accagatttg
    12421 acagaaagct gggaaaaaga ccttgaagct attgctgcag gaaagaaaca agcaggaacc
    12481 ttcttgaatg gaatcgaaaa agaaacaaaa cgcttggtga atgagatcaa atcaagcaaa
    12541 caagaatatc aagatttctc gattacccaa aagaaatgcc cagaatgtgg ggccaatctg
    12601 cgcgagaaaa acacacgtga cggcaaaatc tatgtctgca ccaattcaga ttgcaactac
    12661 cgcagaagaa aagatccaaa agtctctaat catcgatgcc cgcaatgtca ccggaaaatg
    12721 gaaatcatcg aaggtaaaaa cggtgcttat ttccgttgta aatacgatgg aacgacagaa
    12781 aaattgcttg ataaaaaaga gcagaagaag aaaatgacca aacatgaaga acgacgcttg
    12841 ctgaaaaaat atgcacaagc agaagaacca gaagaaagcc cattagcagc agcactaaaa
    12901 gcagcaatga agaagtaata agatatgaaa gaggttgtga tagaagtggt cagcttcaag
    12961 aaataagaag gaatttacga aaattgcttc tcaaattttt gtgaaatttc ggcttatttc
    13021 cgaagaagct acttctggaa caccatttat tcggaaaaaa ggactgtgac aagacttttg
    13081 tcacagtcct ttttcattta aaaaaatgtt tacttcattt gacgtatatt attggtgagg
    13141 cttcaattca tttggcttca ctttttctgt ttctcttatg atataaacag gacgctgttt
    13201 cgtttctaag aagattttcc caatatattt cccgatgatt ccaagacata acaattggat
    13261 tccaccaatg aacaggaaga tcgagaccat ggaaggccat cctgatgtag gatcaccaaa
    13321 caacaatgcc cgcaaaataa tgaacaaaat tgcaatacca gaaccgacac aagacaaagc
    13381 gccgataaat gaagcaatcg ttaacggtgc atccgagaag ttcacgatac catcaatcga
    13441 gtagttgaat aatttccaga aggaccaaga tgtttcgcca gccacacgtt cgcggttttc
    13501 ataagaaata tattctgttt taaatccgac ccagctaaat aatccttttg aaaatcggtt
    13561 gtattctgtt aattctagga cggcatccac catttgacga gtcatcaagc ggaaatcacg
    13621 agcgccatcg accatttctg tatcgctgat tttattgatc aactggtaga attttttcgc
    13681 aaagaaactg cgtatcgcag gctcgccttc acgagtcgaa cgccgggtgc cgacacaatc
    13741 gatatcagaa gtagtgatcc ggttgatcat ctcaggaagc agttcgggcg gatcttgcag
    13801 atcaacatcc atcacggtca ctaaatctcc tgtagaagct tgcaatccag cgtacagagc
    13861 cgcttctttc ccaaaatttc tggaaaatga gacaaagcgg acataaggat aagctgaggc
    13921 tagcttcttg attgctgcta acgtaccgtc ttttgatccg tcgtcaataa acaaatattc
    13981 gaactcatgg gcacctttga atttttcaac ctcatcaaaa aaaagaggaa cagttttttc
    14041 ttcattaaaa catgggacaa caattgatac tttcatcgtt tctatccttt caatcgtatg
    14101 tttatatatg gtttacattc aaaatttaat aaaccttaaa taacaccttt aaaatttgat
    14161 tttacgtaaa agaacaggta ttctttttgt aaactaacgt atttagttta ccatgtttcc
    14221 tcggtttatc aaacttttaa gaacaatttt tattagttgg caagtagttt tggatagaga
    14281 aaaaggagga agttccagtc tattgactag gagcaaacaa aatgaaaata cacacaccta
    14341 tcggcttttt atggttagat gcagatgaat ccggattgac tgctgtatct tttcagccaa
    14401 tttcagaaag cgaatcagcg aatcagcgaa ttttaacgcg cacacaagaa cagtttgaag
    14461 aatattttgc gggaaaaaga aaagaatttg atgttccttt atctataaag agaggtacgc
    14521 cttttcagca aaatgtctgg aaagctttgt cgaccattcc ttatggagaa atccgaacgt
    14581 ataaagaagt agccgttgct gttgatagtc cgaaagctgt gcgagcgatt gggcaggcaa
    14641 atcgaaccaa tccattgcca atcgttattc cttgtcatcg agtgatcggg caaaatggaa
    14701 aactgacagg atatatggga aacgcaatag atggggttga aatcaaacgc caattgttgt
    14761 tgttagaagg atatctgcaa aattaaaaac agttgtgatt cggacattgt tccatgcgag
    14821 aatcacaact gttttttcat gttcattcta aactttgatc agccaaataa atagtaaagc
    14881 catcactgtt tggagaagcc cgacagctaa tccataagat aagaagcgtg taccttcttt
    14941 catgaatttt gcaaagtcta aacgcagacc aattgccgct aaagcagtga tctcaaacca
    15001 gctgctgata aaatgtgccg attggtcaat aactacaggt aaagaaaaca agctgttgac
    15061 caaacaaaag atcacaaacc ctaatacata ccaaggaagc ggtaattttc gttttttctg
    15121 cgtatcagtt ggcgtttcaa acagagctgg ttgtttcttg tgcatgaatt tttcaaacag
    15181 atagacaaca acaacgagca tcataatacg tagaatctta aatagcatgg cgaattcgac
    15241 aacagaattg tcaagtaagc ttgccccagc gactacttga ccaacagatt ggagtgtacc
    15301 acccaaaagt gcactttttt caatgagatt tccaccaaac aactgtaatc caagaaaagg
    15361 gagagtcaac atcatcacag tacccagtag attgactagt gtgatgatct gtcctttttc
    15421 ttcctctttc gcatggatgg aaggggcgat tgaagcaata gcagaagaac cacaaacagc
    15481 gtttcctcca gccatcatca aagaagaagt atcgctgaat tgcagttttt taccaatcaa
    15541 gtaagcaccg atgatagtga ctgacatttg cagtaacaca aaaacaattc cttttacacc
    15601 aatttgagca atcgtttgag ctgtgacagt tgcacctagt aaaaaaacag agaattctaa
    15661 tagtttgctt tcagcaaatt ttgtgccgat tgctagaaca ggctgtttaa aaaaagtatt
    15721 tcctaataaa atacccaata aaatagcaat agtcgctcca cctaactgtg gcagataaat
    15781 agcaagaagc ctgctgaagg cagcgatggc aaaagaaagc agtaaaccgg gtaagatttc
    15841 gcggaccgct gaatccttta cagattttat tggatgtgac aatgatttca tttgatcggt
    15901 tcctttcgtc aataaatact aaaagcatac tcgaacatag tagcactaaa ccagtatttt
    15961 gacaaaagaa caagaggtcg tgacaaaagt cttgtacgac ctcttgtttc gaataaacgg
    16021 tgttccagaa gtagcttcta cggaaataag ccgaaatttc acaaaaattt gagaagcaat
    16081 tttcgtaaat tccttcttat ttcttgaagc tgaccacttc ttttacgacc tcttggtaaa
    16141 cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc agtacaaaaa catgaacagc
    16201 tgtttttgtt tggccgttct tattacttga tgcagaacag ctttttcaca acctattgaa
    16261 tcgattatta agcgattttt ttagaaacaa attttccatg tttgctatgt tctttaatta
    16321 agaaacgtac gtaatgagca agaacgtaga agacaactac acttgcaaac agacattccg
    16381 caacttttaa gaaatccaat acgctactat agatacctaa actgttgaag taatgaacag
    16441 tagtaaatat agctgttgca gagataacaa gcgggaatgt aaaggctgca tagcttggat
    16501 aaaaacgaag cttgaacaaa cgtggaagca tagacagcac aacgaaatac aatacttgag
    16561 ataaagcgaa caatgctacg acaagataaa aattaggttc agcaaagttt ttaagataac
    16621 ctgttaggca aagagaacca ggtgctgcaa cgatcgtaat cagcggacgt gtcggatgag
    16681 ccatatttct tacgaaaaag acacgtttga tgatgatcgg caataagaac gcatagcaga
    16741 tcaatgaaat ccaaaagaaa atttttccaa taagtgggaa gaagttcccg gcagttacag
    16801 tgatgacccc aaatccaacg taaatgataa accagcttgg ataaattgct tcaattgaaa
    16861 gatcagcttt gataacatgg tgataattga aataaaagac aaggatcact tggaagataa
    16921 cagcgcacag ccaaatgaat ttgacaattg gtgcaatact ttcatatgaa acaaaataag
    16981 tacaaataac catgagtgac atcgtaaaag ttggtgaaac ggaggcgatg attggatcat
    17041 gcaacgtttg tttcgtgtgt tcgaataaga taaacaattt aacaaaaatg aatatcataa
    17101 gaatcataga gataccgcca gttagattgc ctaaagcaac atggtgatag tctttaaaca
    17161 gatttccaag ggaagctaac ccaaggatca acccgcagat cgggatcgga ataattttca
    17221 aaaagttctt catatataat attctccttt ttatttaata gattttagta ccaagatgaa
    17281 cgaatacatt gtaaaatgaa taggaatgta tgattacgag caagcagaca gctgattgtc
    17341 ttcatctaag ttactagttg gatcagcaac tgttttgttg tacatttcta ttatagatgg
    17401 ttttttgtat atttaaatca aatagttttt atagtaatta tcataaaaag ttatggaagt
    17461 ggtgccatgt ttaagttatt aacgactttt cgagcagtat atgaaacaag aaatttttcc
    17521 agagcagcag aagtactgtt tttatctcaa ccagcagtct cgaatcaaat caaacagttg
    17581 gagcgggaat tgaatatcga tctttttcaa cgtaacggaa gaaaagagat gcttccaact
    17641 aagcaagccg acttattata tgaacggaca ctggttttgt tagaagaatg ggaagaaact
    17701 cagcaaaaat tgctcaatga acaaaatgaa gaagaagtat gtcggatcgt tgcttcccac
    17761 acctttgctg tctatctttt gcctcagttg atgaaacatc tgatcaaaag gtttcctaaa
    17821 gtgcattttg agatcgaaat cacgaattct catgaatcat tagaacaagt agcgaaacat
    17881 gaatccgatt ttggttttat cgaaaagcca ttagagactt ccggcgtaca aagatactct
    17941 ttgatggaag accagctagt catcgctggg gacccaaaaa gcaaattgtg gcttgtaaga
    18001 gaagcaaact ctggtgtttt tcattacacg cagcgttatt ttgaagagat gaatatccaa
    18061 gataagaaga tgatcgtgaa aagtaacgat gtaattgttg cgttgctgaa ggaaggaatc
    18121 ggtcgctcga ttttgtctaa gcgctctgtt ccagaaaatg tgccaatgac tccgttgaat
    18181 ttccatcgat atttttattt catccaacga accaatctgg tttctgatca gctgctagag
    18241 attgccgaag agatccttcg atacaatgaa agcacagaaa cttctcacag cgaggagaat
    18301 ctcagctggt agataattgc atgtatcaaa aaaagttcta tgatacacta aagataagca
    18361 gtaggctttt gtactgcatt tgctcaagca actgcacaaa cggaaagtta atttttccca
    18421 aatgtctatg gaaagacttt ccattatcta actggaccgc tataataatc agcagtatgg
    18481 tttttaagaa aagggatgaa acatggaaaa aaaattaaaa gcttcgaacg ataatcgtat
    18541 tgattatggc gtgattttac cagtctttct gttatgtttg attggtctgc tctcattata
    18601 tgtagcactt agccatgatc cgaacaatcc agcagtcttg aaaggcgtag ggatgcaagt
    18661 tgtctggtat ttagttggag cggcagcaat cgttgtcatc atgcatttga attccaaatg
    18721 gatctggaaa ctgactcctt acctttatgg tttggggtta gtggtaatgg gactcttgct
    18781 caaattctat gatcagaatt tagctgcttc aacagggtct aaaaactggt ttcgattcgg
    18841 tgcgtttact ttccagccag cagagttgat gaagattgcg tacatcttga tgatggcact
    18901 gatcgtaact aaacataata cgcaagtaaa agaacggacg atgaaatcgg acttttggct
    18961 gattgggaag ctcttgatcg tgacaatacc ggttttagca ttgatcatgg cccaagatga
    19021 ctttggaacg atgcttgtct ttcttgcgat ctttggcggg attttcctga tgtctggaat
    19081 ctcttggcgc atcatcgcac ctgtagtaat cttggcagta gtcgttggag caggaacgat
    19141 cttcttggtt acgactgaag gcggaagaga cttgctttat aaagttggat tcaaatctta
    19201 tcagtttgca cgtattgatt catggctgga ccctttccat gatacgtcag gaatgagtta
    19261 tcagccggcc caaggacttt tggcaattgg taccggtggc ttgtttggta aaggattcaa
    19321 cgtaagcaat atctatgtgc ctgttcgtga gtccgatatg atttttaccg ttatcggtga
    19381 aaacttcggc tttatcggtg gtgcattcgt cattttcttg tattttattt taatctatcg
    19441 tatgattcgg gtgtgtttcg atacgaataa tgaattttac gcctatatcg caagcggttt
    19501 gatcatgatg ctgctgttcc atgtctttga gaatattgga gcaaatatcg gattacttcc
    19561 tttaacaggg atcccattgc ctttcattag tcagggagga tcctcgattt tagggaacat
    19621 gatcggtatc gggttgatat tatcgatgag ataccaaaat gaagcacctg caagatcttc
    19681 aaggaggtaa gaaaattgta caccttttac tggtacccga aatgttcaac atgcaaaaaa
    19741 gccaaagcgt ggttagacca gcatggcgtt agttatcaaa ccgttgatat gatcaaagaa
    19801 gtaccgaagg cctcaacgat tgagacttgg ctagaaacga gtcaagtacc aatccgtcgc
    19861 ctgttcaaca caagcgggat gaaatatcgg gaacttggac tgaaagatca agtaggcgag
    19921 tttacggctt caaaagcgag ccaagtctta tcgacagatg gtatgctgat caagcgacca
    19981 ctgatcttac gagaggacca gcttgttgca attggattca atgaaaaaat ttatgaagga
    20041 gaactgatat aaaatggcag gcaagcattt gaagaaaaag gacaaccttt ggatcttatt
    20101 taatggaaaa gaatactgtg ttggtttgac aaatgaagcg caagaagaac ttggagacat
    20161 cacgtttgcc aatctgccaa aagcaggaca atcgctaaaa aaagatgaac ctctgatcga
    20221 agttgaagcg gaaaaagcgg tcaatgaatt tgccagcccg ctaacgggta cagtttcttc
    20281 tgttaatgat aaaatcggtg aagatatcaa tatcttgaat gattcagatg aaatgaatgc
    20341 gtggatctta agtttcaaag atgttgatcc aaaagaattt gacgaattat aaaatcagaa
    20401 aatcacttga aaaaagttga cactgattct tccagttgct ataatcataa gcaatcctat
    20461 atgaaacgag aaagctttga aaagaagagt acatgtcacg gacgtttgaa gagagccctg
    20521 tttgctgaaa atgggtaacg aaaatgagat ggaagatggt cttagagcag agtaagcgag
    20581 cgtaagcaaa cttactacgg gagtgcccgt tacagcgcga cagtatacgg aaagaagcta
    20641 agtccaagta ctggaagagg ctgttattgc gaagtaacgg taaatcaagg tggtaacacg
    20701 gaagttcaag ctttcgtcct tttgcttaag gcaagagggc gaaggctttt ttgtttgaaa
    20761 aatcataaga gaaagatgtg aatggcatgt cattaatcca actaacgaat gtcaaaaaga
    20821 cattcaatgg gaaaaacgga aacatccaag ccgtcaatga cgtttcacta aacgttgaaa
    20881 aaggggatat ttatggaatc gtcggctatt ctggcgcagg aaaaagtaca ttagtccgtc
    20941 tcctgaatgg acttgagctt cccacgagcg gcgaagtgat cgtaaacgat caagatatta
    21001 caaaattaaa aaacaaagaa ttacgtacat tccgaaagaa aatcggtatg atcttccagc
    21061 atttcaacct gttatggtca agaactgtac ttgaaaatat ccagctgccg ctggaattag
    21121 ctggcgtacc aaagagcaaa cgtaaagaac gggcagaaga actgttgcgt ttagtcgggt
    21181 tagaaggacg cggacaagca tatccttcac aactttccgg cggacagaaa caacgcgtcg
    21241 gtatcgcacg agcattggca aatgatcctg agatcctgct ttgtgatgag gctactagcg
    21301 cattggaccc gcaaacaaca gaagaagtat tagacttatt gttagcgatc aataagaaat
    21361 tgaatttgac catcgtcttg atcacacatg aaatgaatgt gatccgcaaa atctgcaaca
    21421 aagtcgctgt gatggagttg gggaaagtcg tagaagaagg agacgtattg actgtcttca
    21481 gacaaccgaa aaaagaagta accaaacgct ttgtacaaca agacatcgaa ccagaagctg
    21541 attcgacaga actattagct gaactcatca aagaaaatcc aagcggccgt ttggcaactt
    21601 tgacctttag cgaaagaaat gccaacgagc cagtcatttc ccaagcgatt cgacgctata
    21661 acatcgatgt caacgttgtg tatggaaaga tcaaacaagt gaaagaaggt tcctttggtt
    21721 cattgactgt cttgatgact ggtgaaacca aagaattaga tcaagcagaa gtgttcatca
    21781 aagaacaagg agtaggaatt gaggtgatcc atcgtggata aaagcttttc agaaacatat
    21841 ctagatttta aacaaatcaa cccggaaacg atgaatcagg ctatccttga tacactatat
    21901 atgacattaa tctcaatggt cttagtgacg atcatcggat tagttttggg attgatcctt
    21961 tactctattg gacgcaaaaa caaaccgtat gctcgaatca tttatggaat cgtatcgatc
    22021 atcagtaata ttttccggtc aacgccattt atgatcctga tggttttgat cattccattt
    22081 actaaagcaa tcgtaggcac tatgctaggt gcgcaagcag ccattccagc tcttgtttta
    22141 tccgcagcac cattttatgc ccgtctagta gagattgctt ttcgtgaagt cagccctggt
    22201 gtactggaag cagcagatgc aatgggagca agttactggg aaatcatctg gaaggtcttg
    22261 atacctgaaa gtattcccgc attgatttct ggattaacag ttacaacgat ctcgatgatc
    22321 ggatttactg cgatggccgg cgcgatcggc gcaggtggac ttggaggtct tgcttggcag
    22381 gaaggctacc agcgtggtaa tctaacagtg acattcgttg cgactttaat tattttacta
    22441 atcgtattta tcgttcaagg aatcggtgat ttcttaacta agaaaacaga caaaagataa
    22501 taggatatag ggggatgtag gatatgaaaa agaaaatttt tggatttgca gcagcgcttt
    22561 tgctgactgt aggattagca gcatgcggta acaacagcga cagcaaagat tcagcaaata
    22621 aagcagacga cacgacattg aaagtcggcg cttcaccgac accgcacgca gagattttgg
    22681 aacatgtgaa accattattg aaagatgaag gaatcgatct tgaaatcgtc aaattcgatg
    22741 actatgtatt accgaaccaa tctttggaag aaggcgacat tgatgccaat tacttccagc
    22801 atattcctta tttgaacaaa gaaatcaaag aaaaaggata tgactttgtc aatgccggtg
    22861 ctgtccacat cgaaccaatg ggcttgtact ctaaaaaagt caaagatgtt tccgaattaa
    22921 aagatggtgc aacaatcatt acttcaaatt ctgaatcaga ctggggacgc atcatcacaa
    22981 ttctacaaga tgctggctta gtcaaagtca aagatggagt agatctggaa acagcaacat
    23041 tcgatgatat tgaagaaaac cctaagaact tgaagttcaa ccatacgatc gacccatcac
    23101 ttttagcttc tacgtaccaa aatgatgaag gtgacctagt tgcaatcaac gcaaacttcg
    23161 cttacggcgc agggttgaat ccattagaag atgccgtatt gcttgaaaag gacaactctc
    23221 cttacgtgaa tattattgcg actcgtaaag gtgatgaaga cagtgacaaa atcaaaaaac
    23281 tgattgaagt gcttcaccgc aaagaagtcc aagaatggat cacagacaaa tggggcggct
    23341 ctgttaaacc agtaaaggct gatgcaaaat aaggtatgga acaaaaaaga tgagaaataa
    23401 gtctgtaatg gcttatttct catctttttt atacctattc taatttagaa atcaaaattg
    23461 tttaataata aataaaagat atttgatttt agtaaggaag gtcattgcgc ttcattctca
    23521 attagtctaa aatgagtgag aacgaaagag aatttggggg tataatgcat gtctgtctta
    23581 gaaataaaaa atctacatgt gtcgatcgag gaaaaagaaa tccttaaagg cgtaaaccta
    23641 acaatgaaaa ccggagaaat ccatgccatc atgggaccta acggcacagg aaaatctacc
    23701 ttatctgccg ctatcatggg caatccaaac tatgaagtaa cagaaggcga aattttgttt
    23761 gacgggaaaa atgttttaga cttggaagta gatgaacgtg cgcgacttgg cttattttta
    23821 gctatgcaat atccaagtga gataccaggt atcacgaatg cggaattcat gcgtgcagcg
    23881 atcaatgcga aacgtgatga aaacgacaaa atttctgtta tggcattttt gaaaaagtta
    23941 gatgaaaaaa tggctttact aaatatgcca gaagaaatgg ctgaacgtta tctgaacgaa
    24001 ggtttttctg gcggagaaaa gaaacggaat gagatcctgc aattgttgat gttagaacct
    24061 acatttgcaa ttttagacga aatcgattca ggtctggata tcgatgcttt gaaagtcgtt
    24121 gctaaaggag taaatgaaat gcgcggagac aatttcggtg cattgatcat cacccactat
    24181 caacgattat tgaactatat cacacctgat gtcgttcaca tcatgatgga aggccgagta
    24241 gtcatgacag ggaatgctga cctagctaaa cgtctggaag cagaaggcta cgctggtatc
    24301 agtaaagaat taggaatcga ctataaagaa gaagaagcgt aagggggagc aagaatgaaa
    24361 gaaaaaaact ggctcgatta tcttgacgcg gtaaatgatt tttctttaag taaaggcgaa
    24421 cctgattgga tgagaacctt ccgtcaagac gcactagcga aagcagatga actgccattg
    24481 ccgcatatcg atcgggtgaa attccatcgc tggtcgttat ttgatgtgaa agaaacacaa
    24541 accatcagtg aaacaggcac gattccggca tttgatgcaa tgaaagacaa tcctgtgctt
    24601 gtccaacaag gttcatggac gatttttgaa caactgcctg tggaattagc agaaaaagga
    24661 gtcattttta ccgacttgtt cactgcaatg atcgaatatc cagaactagt ccaagaatat
    24721 tatatgaaaa aagcagtaaa catgaacgaa gaccaattga ctgcactcca tgtagctttt
    24781 atgaacagcg gtattttcct gtatgtgcct aaaaatgtcg tgatcgatga accactcgag
    24841 tccttattca ttcaagatgg cgcatccgat gagcacttct tcaagcatgt gttgatcgtg
    24901 gcagatgaac atagtgaatt ttcttatctg gaacgtttcc agacaaccaa agaacaagta
    24961 gcaaaatcat caggtaatat catcgtggaa gtcatcgcta aagctggttc aaaaatcaaa
    25021 tattcagccg tagatcaatt aggagaaaat attacttctt atatgaatcg ccgtgggcac
    25081 attttacgag atgcttctgt cgactgggca attggtgtca tgaatgacgg tcatgtgatc
    25141 gctgactttg actcagattt agcaggagaa ggtgcacacg cagaagtcaa aatcgtggca
    25201 atcagctcgg gcagacaaat ccaaggaatc gatacacgag taaccaataa agcccctcat
    25261 acgatcgggc atattttgca acacggagtg atccgagaaa aaggaacatt gacctttaat
    25321 ggtatcggcc acatcttgaa aggggcaaaa ggtgcagatg cccaacaaga aagtcgtgtc
    25381 ttgatgcttt ctgataaagc acgtggtgat gcgaatccta ttcttttgat cgatgaaaac
    25441 gaagtaactg ctggacatgc tgccagtgta ggacgagttg atccagaaga aatgtactat
    25501 ctgatgagta gaggattgca taaagaagaa gcagagcgct tagtgattcg aggattcctc
    25561 ggttctgtct taacagcaat ccctgtagaa caagttcgga aagaattagt cgaagtaatc
    25621 gaagggaagt tgaatggatg atcgatgcta aaaagctacg caatgatttt cctatcttat
    25681 ttcaagacgt caatgatgaa cctttagttt atctggataa tgcagcgacg acccaaaagc
    25741 caaaacaagt actccaggca ttagcgtact attacgaaca cgataacgcc aatgttcatc
    25801 gcggtgtcca tacgttagca gaacgcgcga cccatgatta tgaacaagca agagaaaaag
    25861 tacgggcgtt catccatgca aaagaaacag cagaagtgct gtttactcgt ggtacgacga
    25921 caagcttgaa ttgggtagcc agaaccttcg gcgaccgcta tatcaaagca ggagacgaga
    25981 tagtgctctc ttacatggaa catcattcta atgttatccc gtggcagcag ttggcaaaac
    26041 gaacaggtgc tgtcttgaaa tatatcgaac taacagaaga aggcttcctg gatatggagg
    26101 atgcagcgaa aaagattacg gacaaaacca aaatcgtctc cattgctcat gtatccaatg
    26161 tcttaggtgt cttcaatcct gttcaagaat tagctgaact tgtccatgaa aacggcgggg
    26221 tcatcgtagt agatggtgct caatctgccc cgcacatggc cattgatgtc caagcactag
    26281 actgtgattt ctttgctttc agcggacaca aaatgtgcgg accaacaggt attggtgttt
    26341 tatatggtaa acgaaaatgg ttagaagaaa tggaacctgt tgaatttggt ggcgaaatga
    26401 tcgattttgt cgaattatac gacagtacat ggaaagaact accttggaaa ttcgaagcag
    26461 ggacaccaaa tatcgccggc gctatcgcat taggccatgc aatcgattat ttgcaaaaac
    26521 tcggtatgga caatatccat cgatatgaag aagaactagc cgcttatgtg ttgccaaaat
    26581 tacaagcaat tgatggattg actgtttatg gtccacaaga cccgaatttt cggacaggag
    26641 tcgttgcatt caatttggat gggttacatc cccatgatgt cgcgacagct ttagacatgg
    26701 aaggggtagc cgtcagagca ggtcatcatt gtgcgcaacc gctattgaag taccttgaag
    26761 tatcagcgac tgctcgcgcg agcttttatt tctacaacac aacgcaagac gccgaccgtt
    26821 tagttgaagc aatcatggca acaaaggagt ttttccaaca tgggactatc taaattagac
    26881 aatttatacc gtcaagtgat cttggatcat tcttcccatc cgcatcatcg tggaagcttg
    26941 actgactcga caagcaagat cgaattgaac aaccccacat gtggagatgt gatcgaattg
    27001 caagtcgatg taaaagacgg aatcatcaaa aatatcgcct ttgacggcag tggctgctcc
    27061 atcagtacgg caagtgcctc gatgatgaca gacgccgtca tcggaaaatc aatagaagaa
    27121 gcagaacatt taactgtaga ctttctacag cttgtccaag gaaaagaagt caaagatccc
    27181 gaccaattag gcgatgctgc tatgcttggt ggtgtagcta aatttcccgc tcggatcaaa
    27241 tgtgcgacac ttgcctggaa agcactagga aaagcaatgg aaaacgatgg taccgcagct
    27301 ttccatcatt accacaagga agaggagtga gaacatgggc gtaccagaat tagaagaata
    27361 taaattcggg tttcacgatg atgtcgaacc ggttttcagt acaggagaag gcttgacaga
    27421 agaagtgatc cgtgagatgt caagaatcaa aggcgaacca gaatggatgc ttgaattccg
    27481 tctgaaatct ctagaaacat ttaacaaaat gccaatgcaa acatggggcc ccgacctttc
    27541 agacatcgat ttcgatgcca tcaaatatta ccaaaaacca agtgacaaac ccgctcgtga
    27601 ctgggaagac gtaccagaaa aaatcaaaga tacatttgaa aagattggta taccagaagc
    27661 tgaacgagct tatctagccg gtgcttccgc acaatatgaa tcagaagtcg tgtaccataa
    27721 tatgaaagat gaattcgaaa agttaggaat cgtctttact gacactgact ctgctttgaa
    27781 agaataccct gaattattca aagagtactt ttcaaaactt gtaccgccaa ccgacaacaa
    27841 attagcagcg ctgaattcag ccgtatggtc aggtgggacc tttatttacg tgccaaaagg
    27901 cgtcaaagtc gatgttccat tacaaacgta cttccggatc aatgcggaaa acacggggca
    27961 attcgaacgt acgctgatca ttgttgacga aggagcaagc atccactatg tcgaaggttg
    28021 tacggcacct acttattcaa gtaacagcct gcatgccgcc atcgtagaaa tctttacaag
    28081 aaaagatgcg tattgccgtt acacaacgat ccaaaactgg tcagacaatg tgtacaactt
    28141 agtaaccaaa cgagcaaaag catacgaagg cgcaaccgtt gaatggatcg acggaaacct
    28201 aggtgcaaaa acaacgatga aatacccaag tgtttactta gatggaaaag gtgcacgagg
    28261 caccatgctc tccatcgcct ttgcaggagc aaaccaaatc caagatacgg gtgcaaaaat
    28321 gatccacaat gcaccaaata catctagctc tatcgtctct aaatcaattt caaaagacgg
    28381 cggagaagta aactatcgag gacaagtaac ctttgccaaa aacagtgcgg gttcgatctc
    28441 tcatatcgaa tgtgacacga tcatcatgga cgaatggtcg aaatccgata cgatcccatt
    28501 caatgagatc cacaacagcc aagtctcttt agaacacgaa gccaaagtct caaaaatctc
    28561 agaagaacaa ctctactatc tgatgagccg aggcctgacc gaatctgaag caactgaaat
    28621 gatcgtcatg ggctttgttg aaccattcac caaagaactt ccaatggaat atgcagtcga
    28681 actcaatcga ttgatcagtt atgagatgga aggcagtgtt gggtaaacct gttataggag
    28741 ctagaaccct tgatatataa gggttctagc ttttttgttt tgcctgtata ctagaaatga
    28801 ccagattttg accagctctt ttactgatga gccatcatat cagcaaagtc ttgacctgtt
    28861 ttcttaggtg cattatttgt aatatgtgca tagatgtcta atgtcacttt actattttca
    28921 tgccctaatc gttgttgaac taccttgaca gattctcctg cttctaataa taaactagca
    28981 tgtgtatgtc taaacccatg aggtgtaata cgaggcaagt tatatttttt caaaatatat
    29041 cttaaccaat cattaacagt gtttggaatg tatagagtgt ttttaactgt tgtaaaaaca
    29101 tgttgctctt tagagctagt attgtatcca agttttaaat actcttcttt ttgctgtaat
    29161 ttccagttgt tgaggataga aagagtctca gtatctaaag agataactcg tctagaagct
    29221 cttgttttcg gagattgaat tataacttta ccaaattcat ctacagcaag agttttattg
    29281 acatgtagtt ctttattaaa cgtgtctatg tctttccact gtaaagcgag tacttcactc
    29341 tttctcatac ctgtgtatgc caataatctg aagaaggcgt attgtttcat gttaccaaaa
    29401 tctttaaaag catccagtaa attattaagc tcctcttttg agtaaaattt acgatttttc
    29461 tcatactctt tttttcgtgg aagaagtgtt ttttccatag gattattgtc aatatattgc
    29521 attgcaaccc cgtagcgtaa aatttgtgca gtctgttttc tcatataatc gtaactttca
    29581 tatttactgt gccattcatc taccactttt tgacaatatg ggacagagat attcgatagt
    29641 ttaagcttac caaatgctgg aagtatttga ttatttgcgt atcttactga gattgcgatt
    29701 gttgaagctt taacactggt acgctgatga gttatccatt cattatacag gtcttcaaat
    29761 gtcatatcag gtgctttctt tttaacaccg tataatattt gatgtctgta ctcgtcataa
    29821 tctttttgag cttcttgttt gctactaaaa cctcttctcg tagtatttat tttttcacca
    29881 gttcgtggat cggtggcaat gtgtccttta tacatccacg ccacttgacc gtttttcttt
    29941 ttgtactttg atatttttgc cattaaatca tctcctctta gtttacaagg agtacttacc
    30001 actagatggt atcacctccc ttcatttttt tcttagtgta ttttttccca attgatgaaa
    30061 tcgtttgttc tagcttgttt atattatcag taatttctct cacagcttct tcgggataag
    30121 acatgtcttt caattgaata ggtagccatt catctaccat tctttctagt agttcttcta
    30181 tactgctagt gaggaaaaga atgttattgt caaattcttc tacaatagca ttgatgaata
    30241 attcttctat ttttaagata ttgaaaagac taattttttc atttttaact ttattacaag
    30301 tcttgttagc aataaattct tgcatctctg gttctaattt ggaatatcta tccagccatg
    30361 tatctaaatt agagtgtact ggtgggaact gttgacgaag atgctggaac aggtttttat
    30421 ctggaaatgt tataccatct agtacaatgc tatctctata aagaataatt cccaaaatat
    30481 attcttctac attgccacaa actaactcct ctatactcat atttcccaaa tcagcaattc
    30541 gggataatcg ctctggtatt ggtaaatagg ttcccttctc ccattcgtag atagtttttt
    30601 tgtcaattaa ttgaccgttg ttaatcataa gaccaaatgc tggttgtgtt aattttttac
    30661 tctttcttat agcttttatt tttgttccga gtatcttttt tcgttctaaa taatttattg
    30721 gtggacgttt tatcatattg accacacctc ctaaaattag tatacctcaa aaaaaggggt
    30781 gttgtaaata aattttacaa agctgttgac accccttttt tatcgaggta ttatgtgtat
    30841 gtcgatgaac tggtaatcat tttttgtttg attaatcggt taataacaaa atacttttat
    30901 tttgagcaaa aaatacgaag ataattctta gaaaatttgt tcaagcaatc acgaaaggag
    30961 ggggaaatat gtctctacaa atcattgata gcattgtttc tatacaagtt gaacaacaag
    31021 tgaaagagat aattacagtg ctaaagccta tgctagaaga cattatttct ggagtcactc
    31081 tagagtatat ggactatcag caggcaagtg aatatctagg tgtatcagta ggaacgatta
    31141 gaaaatatgt ttctcaatac gggttacctg ttataaaaat tgatacggtc attagattta
    31201 aaaaatctga tattgatagt ttcatggaac ggtataaagg ttgagtaaac aaataagact
    31261 aaaacatata gtaataaaga gtttgttttt attatctgta tgttttagtc tttaacagaa
    31321 ttttataagg aggaaacaca aatggaaaca tcaatgatta atattccaat ttatgtagat
    31381 caagaaaaat tattagagaa gccagttaca aaaaaagaaa aaactgatgt aagagatagg
    31441 tgtttagata actatagaga agttacaaga gatgagtttg tagagatact aaattcatca
    31501 caatcattta ttccaagcaa gacaaagaat aaaggcaatg acaaggagag ttttgtggaa
    31561 accagagtaa ttattctgga tgtcgataac actgttaaag atgagaaggg gaaggtagta
    31621 gatttgagta aagatgatag tagatactta agcatagaaa aggttttatc tatcaattta
    31681 gttcataatt ctgcgtttgc gatacaaaaa agtattcggt actctgaaaa tctagaaaaa
    31741 tacaagattg tctttctatt aaaagaagca attactgact atggtaaaat ggttgctgtt
    31801 tacgaatact tgaaaaagaa catccccggg tgtgatgaca atgtaaatgc ttcgaataga
    31861 atgttttttg gaggatataa gtcagatgca gtgataataa ttaatgaaga caacatgtta
    31921 gatattactg agcttccgat tgattttacc atgacatgtt ctaaaaattg tcaaagtgat
    31981 tcagggagag agtatgaact agtcaatgaa accgattttg ttaagttgat aaaaaatgac
    32041 gacaaagaag aaatgaaaca gtggtttaaa gactgcctgt ttgattcctc cgacatgtct
    32101 tttaatgaga tttatgaaag attgttaacc attgatatga ataaattact taagtcaagt
    32161 aaaaacttaa gatgtctgtt tcacaacgat cataatccct ctgctagtat ttttaccagt
    32221 aaaactgggc attccatttt ttattgtcat tccagtaatt gtgatatcag tgaaaatttt
    32281 attggagttg tgatgctaat tttaaagaaa gacagtcggg tagaaacatt taattggcta
    32341 ttagagaacc tagacttgta tcctacacat tttcgaaaat tgaaagagga atcaaaagta
    32401 ctttttgaca ctcttgaatc aatgaaagac aaacttttca ggacaataag gaattatcta
    32461 gaggaaatgc gacgtgtcta tgatgtcttg ctttctgatg ttaatttttt tgatgatagt
    32521 aaggaaagtg tgacttgtat tttgtcgggg gaacaattag ctaaaaaaat gagttcttac
    32581 tatggtaaaa cttatgacat agataaatgt aataagttac tatcacttat gacttttata
    32641 ggattgataa aaaaattaga tgatgaagat gttcctttga agatgctcga atacctaaat
    32701 aggttaaagg aagagaaagc gttaggaaac tttaatcata agagaagcaa tgtctatcga
    32761 ttagacgttt ttaatgcaac ttctgtgatt caaaaattga atgaagatat ccttccactt
    32821 ctggaaaaaa ttcattttac atatcaatat ttttcttatg aatgggttaa gatatgtttt
    32881 aacgaaaaag aggctaaaag agtatttcct caaaattccc gatcagaact cagcaaggaa
    32941 aaacaattga ttttagatga agcacatgaa gttttacagg gcttatcgtg gcaagggaca
    33001 tatctaatga cagagaaaga acttattaac actgtttgtg aacgcctacg taatgttggg
    33061 gataacagcg tgaaaaatgc tttaataaaa aacagaggct actttgttaa acaaggattc
    33121 actcttgaac gagcttctaa ggatattaag aaactataca atgtgaaagc aagtagtagt
    33181 tttttaatat atttagcgaa aggggcaggg atagatgaaa caataaaaga attgatatta
    33241 tcaggagctg ggatagttcc tctgtatcaa tttacaagtg taacaagaga tgatggacgg
    33301 agagtaagga agagaaaaga cccaattgta ttaaactaga caggttatcc atagctctta
    33361 atcttatttc atctcaatac acccaccgtt aaagttcggt gggttattta tttttcaatc
    33421 tttcaatagc tgattgcgtt cattttcttg cctttttaat agcttcatat agtcaattgt
    33481 tcgaactgca tcttcagtgc taagagaacg atagagagcc aaaaactctt cctcataatc
    33541 tacgacaaca ttgtctagaa aatggcttac aggggttcct agggctctag aaagagctgt
    33601 taagctatcg aggttaggct ctgttacaga attttcccat cttgctactg cctgttgtgt
    33661 agagaagatt ttttcagcta attctttttg tgtccacccg agagcttttc gcttttctct
    33721 aatgaccttt gcatattgtt tctgtttcat taatacaccc ccatctcagt atagtacaac
    33781 aagttgttgt atttgtaatg gtatttgaag ttttttatat tttatgagtt gaaatacatc
    33841 atattgttgt atataataaa acaggagggg agaatcatga aaaaagtaag taatctcagc
    33901 cgatacagaa aagaaaaagg actttctcaa acggagttag caaaaaaaat gaatgttaca
    33961 cagcaatgtg taagttcgtg gcaaacaggg agaacgattc ctaaacccta tcaaatgaag
    34021 atgctttctg agatattaag tgtaccaatg aatgagctgt tttctgatgt atttaataaa
    34081 gtaaacagtt aactaataag caattgaatg attttaggag gagaagacat gtattgtaga
    34141 aaatgtggca aagaaataac agatgaaagt aagtattgcc agttttgtgg agcactacaa
    34201 gaaacagatg agacaaccgg aacagacaca gtaaagaaaa gtactgagca gacaattaat
    34261 gaaatgaagg aacaaatcgt acatggacaa gagacaggga agctgtattt gattattggt
    34321 tggatttcta tggtagtatc gttagcattt attcctgttg tatttggtgc tgttgctgtt
    34381 attatgggct acttatatcg agaaaaagat gagaaaatgg gtacgatttt gatgatagca
    34441 ggggtagctg gagcaatctt tggtgtactt ttagggatgt caatgtactg ataagaggga
    34501 taagaacaaa ttattttgga gataggagat aaaaagttga ataaagtaaa tatgaacaag
    34561 aagtttgtca ttataggtat tgttgtcttg gctattatag gtattatttt ttcaaaaatg
    34621 acaggtacat ctggattaga gaagtcatca aaaccattgg taacacagat tttacaagaa
    34681 cagtacaact taaatcgatc atgtgatgat gtgacgatta ctcatgaaaa tggagataac
    34741 acataccggg ctaaagctat attagacaat ggaagtgcta tcaacataaa tattgagtac
    34801 tatccaaaga aagaccgtat atatgtggag ataccatatg ctgaggtgtt aatgttaaat
    34861 tgatttaaat gtattatgca taccgtccaa acaggaactc ccaaaggtgt aaattcacta
    34921 gggagttttt atagtttcct ataaattaaa tactaataca ggtgacaaaa aaatattaca
    34981 atgattgtcg ggaatttt
//
LOCUS       DAMYXM010000023        33818 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.024, whole genome shotgun sequence.
ACCESSION   DAMYXM010000023 DAMYXM010000000
VERSION     DAMYXM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.024"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            299..1960
                     /locus_tag="PNS21_001303"
     CDS             299..1960
                     /locus_tag="PNS21_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI5159020.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="PNS21_001304"
     CDS             complement(2030..2377)
                     /locus_tag="PNS21_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDI5159021.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="PNS21_001305"
     CDS             2461..3402
                     /locus_tag="PNS21_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5159022.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="PNS21_001306"
     CDS             3403..4446
                     /locus_tag="PNS21_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5159023.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="PNS21_001307"
     CDS             4452..5522
                     /locus_tag="PNS21_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5159024.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="PNS21_001308"
     CDS             5512..6465
                     /locus_tag="PNS21_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI5159025.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="PNS21_001309"
     CDS             6477..7487
                     /locus_tag="PNS21_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI5159026.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="PNS21_001310"
     CDS             complement(7772..8593)
                     /locus_tag="PNS21_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDI5159027.1"
                     /translation="MNRFASKEEEKLYYEHEASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="PNS21_001311"
     CDS             complement(8690..8968)
                     /locus_tag="PNS21_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159028.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="PNS21_001312"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="PNS21_001312"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDI5159029.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="PNS21_001313"
     CDS             complement(11724..12311)
                     /locus_tag="PNS21_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159030.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="PNS21_001314"
     CDS             12486..13808
                     /locus_tag="PNS21_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI5159031.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="PNS21_001315"
     CDS             13963..14739
                     /locus_tag="PNS21_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDI5159032.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="PNS21_001316"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="PNS21_001316"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDI5159033.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="PNS21_001317"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="PNS21_001317"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDI5159034.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="PNS21_001318"
     CDS             16629..17258
                     /locus_tag="PNS21_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159035.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="PNS21_001319"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="PNS21_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDI5159036.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="PNS21_001320"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="PNS21_001320"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDI5159037.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19445..19981
                     /locus_tag="PNS21_001321"
     CDS             19445..19981
                     /locus_tag="PNS21_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDI5159038.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="PNS21_001322"
     CDS             complement(20021..21784)
                     /locus_tag="PNS21_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5159039.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="PNS21_001323"
     CDS             complement(21787..23502)
                     /locus_tag="PNS21_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5159040.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24281..24505)
                     /locus_tag="PNS21_001324"
     CDS             complement(24281..24505)
                     /locus_tag="PNS21_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159041.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24696..27473
                     /locus_tag="PNS21_001325"
     CDS             24696..27473
                     /locus_tag="PNS21_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDI5159042.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            27555..27974
                     /locus_tag="PNS21_001326"
     CDS             27555..27974
                     /locus_tag="PNS21_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDI5159043.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27986..28456
                     /locus_tag="PNS21_001327"
     CDS             27986..28456
                     /locus_tag="PNS21_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDI5159044.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28473..29252
                     /locus_tag="PNS21_001328"
     CDS             28473..29252
                     /locus_tag="PNS21_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDI5159045.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29239..30060
                     /locus_tag="PNS21_001329"
     CDS             29239..30060
                     /locus_tag="PNS21_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDI5159046.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30077..31126
                     /locus_tag="PNS21_001330"
     CDS             30077..31126
                     /locus_tag="PNS21_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDI5159047.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31139..32143
                     /locus_tag="PNS21_001331"
     CDS             31139..32143
                     /locus_tag="PNS21_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDI5159048.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32776..33795
                     /locus_tag="PNS21_001332"
     CDS             32776..33795
                     /locus_tag="PNS21_001332"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDI5159049.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        11041 a         5992 c         6909 g         9876 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga ggttttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gcttcatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
    23701 gaacgtctat tttacttaat ttttctataa atgaattctg cataaaaaat atatcaagta
    23761 aatgtttata gatgaaactc tatttacttc ttttttaatt tgttcttgca atcttttttg
    23821 ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta attgattttc aaagaaggag
    23881 tatgaaaatg agaaaaatat ttattcgttt attgtgggta ctatcttaga ggaataaata
    23941 atagtggaga aaactatggt ttggcgcaga ttctatcagg tttgatggaa cgaagacaac
    24001 ctcccgacat caagtcaaaa gctacttgag attttgatct gaaaataaaa aaagaaaaca
    24061 aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca atgggaaaaa actaagagtg
    24121 gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct ctttactctc tgaaagcatc
    24181 ataactcgtt tttcctcatt atgccaataa attgttgtta taccacatat aaggatttgt
    24241 aatagctaaa aacagacagg aaagttgaaa tctgtgaatc ttattttgta tggaaggatt
    24301 tatagaacgc aaaaatcaag ataaagatga ataaaaatcc tgtttcggct atatattgcg
    24361 tatctacgcc aataaaacga gtttgttctg tcatagccat ataaagcaaa ggcaaaaata
    24421 caactacagc gagataagat ccgatgattt ctcgaaaagt tttccaggaa gtatgtttcc
    24481 tgtttttata tttatccgat ttcattgaat agtcatagcc tccttattct ttcatatgtt
    24541 gaaattccag tataaaaact ggaaggaact gtacgttttt tagaataaaa attataaggg
    24601 ataagtaggc tgctgtctat aataagatat atttttcaat tctttattaa agattttatt
    24661 gacaaaatga atccgttttc tatgatggag gaggggtgaa taagttgttg aagaaggaaa
    24721 ttttatgtta cttagagaac cagacagcct ttttcgattt agaacatatg aacgaggtct
    24781 ttacagcaca aaggttagct gatcattttg gggtaaaaag aaacacagtc agtcattatt
    24841 tgaaccagtt aaatgatgag caactactag tcaaaatcaa ttctcgacca gtgatttatt
    24901 tccacaaaga agcttttgaa aaacaatttt ttaagttacg cacgaatttt tatttttcta
    24961 tcgatgatct caaagaggaa cagccttttt tcgcgcaacc aaaagactta ttctctttta
    25021 tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca aatcaaaaca gctctttact
    25081 atccagatgg cggattacca cttttgatta caggcgaaag tggcacaggt aaaagttatt
    25141 tagttaattt ggtctatcag tattgtctgc ttcatgatct gctagaagat tctgcaccat
    25201 tcatcacttt taattgtgca caatatgcag ataatcctga acttttgact agcaatttgt
    25261 ttggacatgt taagggagcg tacactggtg cagaagaaaa taaaaaaggg gcttttgaag
    25321 cagcagatgg tgggatcttg tttttagacg aggtacatcg cttaacacca gaaggtcagg
    25381 aaaaactgtt cacctatctt gatcaaggtg tgatttatcg tatgggagaa cctaatttgt
    25441 cacgccggat caaaacacgt ctcttttttg ctacgactga agagattact agtcatttct
    25501 taacaacttt cattcgaaga atacctatcc gaatcgaact acctgcactt aatcagcgta
    25561 gtcgtaatga acggctagaa ctgatttact ctttttttct cgaagaacaa agaaaaatgg
    25621 ggagcccttt gtcagtcagt ggaccagctt tgtcactttt aacaagtcag aaattatccg
    25681 gaaacatagg agagctgaaa aatatcgtca aagtttcagc agcaaaagca tatgctgaac
    25741 agcgtgaaca gcctgaaatc catgtcacga tccatcattt gcaaaaagaa ctacttgcgc
    25801 agcctattac taaaaataca gcacagcaag gaatctatat tacgcaagac cagaccttag
    25861 agcaattaat ggagaagcaa cagcccgaac agcagcgaat cgttaaaagt tttgagcaaa
    25921 tcctaattga tttcaagaaa aataactgtc tgctatattc ttgcgaagga aaattaaagc
    25981 aagaagtgat tcaattattt gattttttgc tgtttgagac cagtagacaa gaaaaacacg
    26041 agctgctggt ttattggaca caatctatcc gagagacctt tcgacaaatg gaaactgcct
    26101 atcaaatcaa attcgatgga aacagtgtct atgccttgag ttactatctc tatcagcgaa
    26161 gcactatcaa atggttaccg gaagaacatg atattatcca actaatcaaa gaattagaac
    26221 aacaagtttc tcagtcttat ccatctagtt atcactatgt ccaacgaatt ctggaattaa
    26281 gtaagatcaa attagatatt gaagacacaa gtatggatcg gattttactg acgatctatc
    26341 taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa agcgatgatt gctgctcacg
    26401 gttatgcaac tgctagcagt atggcaaatg tagtaaatcg tttattacaa gaggatctat
    26461 ttgaatcatt tgatatgcca ctagatgtca caccacagca gattgcttca gaaatccttg
    26521 attattgcga aaacagcgat gtttcaaatg gattgatcat attagtagac atgggatctc
    26581 tgaaagaaat ccatcagttt tttaaaaaac agttatccgt accgcttttg attttgaata
    26641 atgtgacgac acctttagca atcacagtag gggaatgcct gcaaaaaaat gtatcattag
    26701 aagagatagc agaagagact gttcgacaga tccagccaga atggcgactg ctctatccag
    26761 aggaaaacaa acccaaagca ctcattacta cttgcttcac tggaatcggt acggcggtcc
    26821 acttaagtga actgctagaa aaaagcttac ctacggcttg tcagttgaaa atcatcccat
    26881 atgaatatca gcaattaaaa gataagaaaa acagtgaccc attattttcc atatacgaag
    26941 tcgtaggaat gattggcacc acagatcctg atatcaccaa tatcgactat ctttcattag
    27001 aagacctcat ctcgggggaa aagatgtctg tactagctga atggttggac actactatga
    27061 atgcttcaga gaaggaaata ttcaatcagc gcatcattcg taatttttca ttggaaaaag
    27121 tactggattc cgtgactatt ctagatacag agaaggtgat gggagaaatc gatcatttta
    27181 tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa tcccaaaaaa ttagcgttgt
    27241 atgtccatgt gagctgtctc attgaacgat tgatccgaca aatgccaatc gaaacctatc
    27301 aaggattaga gaaatggaag cagtgtcaaa aagaagggtt atctgctatt aaatctgcgt
    27361 ttagtgtcat tgaagagcat tatagtgtca tgatccccaa ttctgagatt gcctatatct
    27421 atgatatttt gagtgaagat acagagtttc tctcgataga agaagaattt tgaacataag
    27481 caagttgttg ccactaattt tggcatgcaa cttgcttttt ttagtatgtg taagtgaaaa
    27541 gaggagatga aaaagtggat agagaaatta tcttagcttc ccatggaaaa tttgcttcag
    27601 ggatattaga ttcattagag ctgatctatg ggaaaaatca ttctattact gtattcgatt
    27661 gctatactga tgagaatttt gatttagcgg aaacagtaag gcagcttttt atgaggtaca
    27721 aagataaaga aatcattgtt ctgacggatc tttttggcgg aagtgtcaac aacgaattct
    27781 tacaatatat caatcgggat catgtctatc tcgttgccgg attgaatttg ccactattga
    27841 ttgagctagt atctcggttg gacgctgaag tagagacaac tgttttgatc cagcaggtgc
    27901 taactgattc aaaaaaaatg atccagtttt gtaacgagcg gctcaatcag aaaatagaag
    27961 aagacgaatt ttaggaggaa gaaaaatgat tgtattgaca agagtcgacc atcggttatt
    28021 acacggacaa gtagcttttt catggacaca aacaataggc gcagactgta ttttgattgc
    28081 taatgatgat gtacctacga atgagatacg aaagacaacg atcaaattag ccaagccaca
    28141 aggagtcaaa ctagtgatca aatcaattga tgactcgatt gccgcgttga aaagcggggt
    28201 gacagataaa tataagttat tcatcgtagt ggaatcgatc gaggatgcat ataagctggc
    28261 agaagcgtat ccagaaatca aaaccatcaa cttaggaggg atcaaggcaa aacctggtac
    28321 tcgcagcatc ggaaaagcgg taaatatttt accagaagag gaggaacgct tgaaacgatt
    28381 aattgacaaa caaatagaag tagaaatccg tcaagtacca gcagataaaa aagtggatgt
    28441 aagagaagtt ttgtaagaaa ggaagaacct caatgattgt acaagcagtc ttattaggaa
    28501 ttgttgcatt tgtcgcacaa tcagaatatg cactagggac atcgttgctt tctcgtccga
    28561 ttgtgacggg gctgtttaca ggaatcgtgt taggtgatgt aaaaactggg atcattatgg
    28621 gcgcaacttt agagttagca tttatcggat cgttctcagt aggtgcttcg attccacctg
    28681 atgttgtgac cggtgggatt ttagggacgg cttttgctat tacagctgga gccggtacag
    28741 aaaccgcgct tttactaggt ttgccgattg ctacgttgac attgatattg aaaaatattt
    28801 acttagggct tttgattcca attatgaacc ataaagcaga caattatgca gaagaaggaa
    28861 attataaagg agtcgagcat atgcacttgc ttgccggtat cggtctttcc ttgatgcttg
    28921 gtgttgtggt aatggtttct tatatggtgg gaagcaatac gatcggaaat ctattaaata
    28981 tgattcctga cttcgttcaa aaggggttgt cagtagctac agggttgatt ccagctcttg
    29041 gttttgcgat gcttgctcgt ttgctgatca acaaacaagt tgcaccatat tttttcttag
    29101 gttttgctat agcttcttac ttagagattc ctgtgaccgg agtagctatt tttggtgcga
    29161 ttttagcagt tgtggtagtc aatattatga atgtccgtca actaccactt caaacaactg
    29221 caggggaggt agaagaagat gaagaattct gagatcaaaa acagcaaaat aacgaaaaaa
    29281 gagctaaatc acgtattttg gcgttctttt caaatggagt tttcttggaa ttatgaacgt
    29341 cagatgaata tggcttatgt ttatgcaatg attccgattt tgaaaaagtt atatcagtcg
    29401 aaagaagaaa tggcagcagc tttgaaacgg catctagagt tttttaacac gaccccacat
    29461 atcgttacac tgattttagg aatcacggct gcaatggagg aagaaaataa ggatgatcct
    29521 gaatttgatg tgactgcgat cgatagtatc aaaacatctt tgatgggacc tcttgcaggg
    29581 ttaggagatt ctttcttttg gggaactttg cgattgattg ccacaggtgt gggtacctct
    29641 ttagccatgc aaggaaatat tttaggccct atcttgttca ttttgatttt caatattccg
    29701 cacattctgt ttcgctatct agcaacaggt tggggatatc gcttaggaac gggttttttg
    29761 aaaaagatcc aggcaaacgg tatgatggaa agtttgacat taggcgcgtc tattattgga
    29821 ctgatggtcg ttggagcaat gactgccacg atgatcacca tcaatattcc tttaaaaatc
    29881 ggttcaggag aaaatgctgt gactgttcaa agtatttttg atgatatcgt tccaaatatc
    29941 ttatgtcttg gagcatttgg catagtgttt tatctattga agaaagaagt gaaaccatta
    30001 acgatcttga tcggactagc tttctttgga atttttggtt cgtggatagg gattttttaa
    30061 aaaaggagca gagaaaatgt ttacgatgca agattatatc tatgaagaaa aggaagtact
    30121 aagtacgatc ttgaaaaaga atgatttttc gacaagagaa catatgaaaa aaacagtgaa
    30181 tttactgatc ttagcaacgg gttcatctta taacgcttgt ttagcagcga agccagcatt
    30241 ggaatcttat ggtgatctta ctgtcgatat tcaagaacct ttttattttt tcaattatgg
    30301 aaaactttct ccttcaatcg atactgtgat tgctgtgtca caaagcggga aaagtgcttc
    30361 tactgttgaa gcagtaaaaa tgatacaaaa acaagggtta cctgttgttg ctatcacaaa
    30421 tgacgtgcaa agtccacttg ctttagaagc tgcacaaatt attgatttag gtgccggggt
    30481 agaatcagta ggcttcgtta caaaaggcta ttctgcgact gttttacagt tactactctt
    30541 agggatagga attggaataa gtaaaaataa aatcagcaaa aaaatagaac aagactacat
    30601 gcaacagttg cgcaaaataa tcaaccattt accagcgatt attcaaaaaa cggaagaatt
    30661 ttttgatgga tatcaaagtt tatttcgttt agcacagcgt ttcgtcacca ttggatatgg
    30721 tccaaactgg ggaacagcta aagaagcaga gacgaaattg actgaaacaa tccgcgttcc
    30781 gtcacaagga tttgagttag aagcttatat gcacggccca tacctggagg cagatgcttc
    30841 gcatttgcta ttctttattg aaggggaaag tgtaaataag gaacgttcgc aaaagttgca
    30901 aagatatatg agtcggtacg ttggagagac tctcaccatt accacaaaaa aagcaagaaa
    30961 cgagaaaaca cttggattag caatagaatg tgatgaatat ctgtctgtat tagcgcttgt
    31021 cgttccgttt caactattcg cttataaaac cgcagtagct aaaggtattg acttgaacaa
    31081 aaagattttt gaagactttg atacagtctt gaaaagtaaa ttataagagg tggaaatcat
    31141 gctgaaattc aatgaagaag aacaaatcaa agcaataaaa ggcgcactag ctttacgtcc
    31201 gcaagtagaa gaaatcatcg ataaacttta tgcggaaaaa tttgacgctg tctattatct
    31261 aggaatcggt ggaacgtatg cttcctcgat gcaagctgtc acttatatga atgggaaaag
    31321 caacttgcct gtctttgtac agcatgctgc agaatattac actactggaa acaaaagatt
    31381 aacaaaagat tctatcgtta ttttgtcctc tgttaccggt acaactcaag aagtagtgaa
    31441 agcagtagaa caaatcaaga aagtaggagc gacactgatt ggatttatcg ataaagcgaa
    31501 cagtaagttg tctcagctat gcgattttgt ggtcacttat ccagctcctg gaacagaaca
    31561 aatcaaattt tttatggcag cagatcgatt gatgtatttg aatggagaat tcgaagctta
    31621 ttcagaatac tatgaacagc tcgaacgtta tttaccaaca gggttagtgg aagcagagaa
    31681 aaaagcagat gcatttggct tatcatttgc agaaaaacat cgacgtgata gtatgcatta
    31741 ttttattgga gcagggaatc aatggggagc tgtttattca tatgccatgt gttactggga
    31801 agaacaaagc tggcttccat cgaaatctat ccatgctgcc gagtttcttc acgggacgtt
    31861 agagatcgta gaagaaacaa ccccagtcac attattttta ggcgaagatg aacagagagt
    31921 gttatctgaa cgtgtggcta aacttttgcc aaaaatttgc agcaattata cattgataga
    31981 cacaaaagac tatccagtag aaggtatcag tgaaaaatat cgcggacgtg ttttgtcttt
    32041 cttattgatg catgtagtga cacaacgcat cgatgctcat gtggagcagt tgaactgcca
    32101 tccattagag atacgccgct actatcgtca gtttgattat taggatagac tggataaaaa
    32161 gaatataaaa aataaagaga gcagcgataa ctgctccctt tattttttta aaatattggc
    32221 atcaattacg ttataaaaca cattttttgc attatctaca gttcaactta taatctgttt
    32281 aatgggacat gtgggtgcca accatttttt gttatgcaat agtactattt agttgttgca
    32341 tggatccgta gtaaaagtcc aagtacaacg agttgcgcca gtttacatag cgttttgtat
    32401 ccagaatgtt gactaaaaca aaagttccta tgtcaaccgt caacagagac aatctgtccg
    32461 ttgcaggacc tgacagattt agaaatgttc aatctgtctc ttatgtattt taaatcaata
    32521 ctcactagga gaagtttcct aacctcctaa tagtaatgct ggtagtaaca ttgtcaaaac
    32581 tgcttcctct taggaacaag caaagcataa cgagtgtaaa aataaaatgt agactattgt
    32641 aacatatcat tgattatttc aagtaaaaac gataaaagaa gtaaacaaac acttaaaaac
    32701 aatggaaatt agtcccaact ttaagaaatg tatactataa tggaaattat caattattta
    32761 tttggaggaa tatccatgaa acaaaaaatc gcagttcttg gtcctggatc ttggggcact
    32821 gctttagcac aaacattggc agaaaacggg catgatgtcc gtatttgggg aaatgtacct
    32881 gaacagattg atgaaatcaa taagtatcat acaaatcagc attttttacc tgatttgacg
    32941 atcccagaat cgattatcgg atataaagaa ttggcagaag cagtggataa cgcagatgct
    33001 attttatttg ttgttccaac aaaagcgatt cgttcagtcg cacaggaact tattggtaaa
    33061 atgaatacta agccaatcat tatacatgca agtaaaggac tagaacaaga tacgcataaa
    33121 cgaatctcag aagttttggc tgaagaaatt ccagaagaaa aaagacaggc aattgttgta
    33181 ttatctggtc ctagccatgc tgaggaagtg gcagtacatg atatcacaac aattacagct
    33241 gcaagtattg atcaaaaagc agcagcctat gtacaaaaac tgttcatgaa tgattatttc
    33301 aggatttata caaataatga tgtgattggc gtagaaactg gtgcagcact gaaaaatatc
    33361 attgcaattg gtgccggcgc tatccatgga ttaggattcg gagatgatgc aaaagcagca
    33421 atcatgactc gaggattggc agaaatcagc cgtcttggtg tcgcgatggg tgccaatcct
    33481 ctgacattta tcggtttgag tggtgtgggg gacttgatcg ttacttgtac aagtgttcat
    33541 tctcgcaatt ggcgtgccgg taacttgtta ggacagggac ataagctgga agaagttcta
    33601 gaaaacatgg gaatggtcgt tgagggtgtg gcaacgacaa aagcagcagt agagctagct
    33661 caacagctag gtgttgagat gccaattact caaacaatct ataatgtttt gtataatgga
    33721 gaagatatca aaaaagcagc aaaagaaatc atgttacgtg acggaaaaat ggagaatgaa
    33781 ttttctcttc gttaaataga tgatcaatag ttaatcgt
//
LOCUS       DAMYXM010000024        33412 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.025, whole genome shotgun sequence.
ACCESSION   DAMYXM010000024 DAMYXM010000000
VERSION     DAMYXM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.025"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            292..1431
                     /locus_tag="PNS21_001333"
     CDS             292..1431
                     /locus_tag="PNS21_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDI5159050.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="PNS21_001334"
     CDS             complement(1498..2073)
                     /locus_tag="PNS21_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDI5159051.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="PNS21_001335"
     CDS             complement(2078..3052)
                     /locus_tag="PNS21_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDI5159052.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="PNS21_001336"
     CDS             3335..4303
                     /locus_tag="PNS21_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI5159053.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="PNS21_001337"
     CDS             4416..4694
                     /locus_tag="PNS21_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI5159054.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="PNS21_001338"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="PNS21_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDI5159055.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5151..5906
                     /locus_tag="PNS21_001339"
     CDS             5151..5906
                     /locus_tag="PNS21_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI5159056.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="PNS21_001340"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="PNS21_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDI5159057.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="PNS21_001341"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="PNS21_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDI5159058.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="PNS21_001342"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="PNS21_001342"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDI5159059.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="PNS21_001343"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="PNS21_001343"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDI5159060.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="PNS21_001344"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="PNS21_001344"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDI5159061.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="PNS21_001345"
     CDS             10643..11185
                     /locus_tag="PNS21_001345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDI5159062.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="PNS21_001346"
     CDS             11208..12764
                     /locus_tag="PNS21_001346"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDI5159063.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="PNS21_001347"
     CDS             12778..13680
                     /locus_tag="PNS21_001347"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDI5159064.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="PNS21_001348"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="PNS21_001348"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDI5159065.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="PNS21_001349"
     CDS             15123..15545
                     /locus_tag="PNS21_001349"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDI5159066.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="PNS21_001350"
     CDS             16014..16244
                     /locus_tag="PNS21_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDI5159067.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="PNS21_001351"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="PNS21_001351"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDI5159068.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="PNS21_001352"
     CDS             17655..17831
                     /locus_tag="PNS21_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDI5159069.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="PNS21_001353"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="PNS21_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDI5159070.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18326..18793
                     /locus_tag="PNS21_001354"
     CDS             18326..18793
                     /locus_tag="PNS21_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5159071.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            18878..19762
                     /locus_tag="PNS21_001355"
     CDS             18878..19762
                     /locus_tag="PNS21_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDI5159072.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19876..20112
                     /locus_tag="PNS21_001356"
     CDS             19876..20112
                     /locus_tag="PNS21_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HDI5159073.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20200..20280
                     /locus_tag="PNS21_001357"
     tRNA            20200..20280
                     /locus_tag="PNS21_001357"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20234..20236,aa:Tyr,seq:gta)
     gene            20287..20358
                     /locus_tag="PNS21_001358"
     tRNA            20287..20358
                     /locus_tag="PNS21_001358"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20319..20321,aa:Gln,seq:ttg)
     gene            20483..20653
                     /locus_tag="PNS21_001359"
     CDS             20483..20653
                     /locus_tag="PNS21_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159074.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            20873..21580
                     /locus_tag="PNS21_001360"
     CDS             20873..21580
                     /locus_tag="PNS21_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI5159075.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(21639..22273)
                     /locus_tag="PNS21_001361"
                     /pseudo
     CDS             complement(21639..22273)
                     /locus_tag="PNS21_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
     gene            22400..22687
                     /locus_tag="PNS21_001362"
     CDS             22400..22687
                     /locus_tag="PNS21_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159076.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            22795..23115
                     /locus_tag="PNS21_001363"
     CDS             22795..23115
                     /locus_tag="PNS21_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDI5159077.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            23196..24509
                     /locus_tag="PNS21_001364"
     CDS             23196..24509
                     /locus_tag="PNS21_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDI5159078.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKVCQEIKEMNEEGKKML"
     gene            24506..25225
                     /locus_tag="PNS21_001365"
     CDS             24506..25225
                     /locus_tag="PNS21_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDI5159079.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYRWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            25353..25904
                     /gene="raiA"
                     /locus_tag="PNS21_001366"
     CDS             25353..25904
                     /gene="raiA"
                     /locus_tag="PNS21_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDI5159080.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            26126..28660
                     /gene="secA"
                     /locus_tag="PNS21_001367"
     CDS             26126..28660
                     /gene="secA"
                     /locus_tag="PNS21_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDI5159081.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            28786..29887
                     /gene="prfB"
                     /locus_tag="PNS21_001368"
     CDS             join(28786..28857,28859..29887)
                     /gene="prfB"
                     /locus_tag="PNS21_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDI5159082.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            29992..30678
                     /gene="ftsE"
                     /locus_tag="PNS21_001369"
     CDS             29992..30678
                     /gene="ftsE"
                     /locus_tag="PNS21_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDI5159083.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            30662..31555
                     /locus_tag="PNS21_001370"
     CDS             30662..31555
                     /locus_tag="PNS21_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5159084.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            31707..33155
                     /gene="cls"
                     /locus_tag="PNS21_001371"
     CDS             31707..33155
                     /gene="cls"
                     /locus_tag="PNS21_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDI5159085.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT        11107 a         5822 c         7246 g         9237 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaatgg ctcatacgga atggcgcaaa
    11401 acttagaatc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggaaagatca tggaagttcc agttggagag gccttgatcg
    11521 gacgggtagt taatccgcta ggtcagccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagca atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcgccgttgc tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga gttcatgtac aacggtaaac atgtcttgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca gcaaaattaa
    12121 gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa atcttcttag
    12241 aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta tctgtatctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgcctag
    12361 acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac ttggatgcag
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaattgc
    12481 atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca catggattct
    12541 tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaagta
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg ttaagaaaac tggttatatc gtcatcactg cagatggcgg tttagtcggt
    13081 ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg ttccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg ctcgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaaaaaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaattg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtagg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataaccgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tggtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgtcgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttag
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg tgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt aacaactccg
    18301 caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag atttaccatg
    18361 gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg tttccagagg
    18421 gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct atgtggcaag
    18481 ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag gaaaggggat
    18541 tggcggaaaa ttacttgaga cagcttttca gaagctttca cagctagctt caaaccaaac
    18601 tcggatcatt atacgtactg gcactactag tagcgctgcg ttacacctct atcaaaaaat
    18661 gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattact ctgagcccat
    18721 ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact tacctaaaaa
    18781 tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa atagatacat
    18841 tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag tttttgattt
    18901 tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag gtaagggaca
    18961 atttgcaacg cctgaatctt gggaagaaat gaaaacgcaa ttatttcaag tttataaaaa
    19021 aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag tagtgaataa
    19081 tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat ttaatatttt
    19141 ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc
    19201 cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg cttttgttgt
    19261 ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaataaata aaggggcaca
    19321 tctttacggt ggagaatttg ggttgatgtt cttagatggc gataagacat tcagtaaact
    19381 tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc
    19441 ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag caaaagaaga
    19501 agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat tttcgtttga
    19561 tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca ttgatgagat
    19621 caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg aaccgcagat
    19681 tttattatgt gagtacagaa acgatgctaa tcttgtaggt gcagcagcaa actatctcgc
    19741 tgtacgtcaa ggagagaatt aagagcttta gagaaggtgc agcttgcata aaagaagaga
    19801 atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt atgataaaat
    19861 atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa
    19921 gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat agggaaaaaa
    19981 atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt tggtgaagaa
    20041 gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg acactggaat
    20101 ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa
    20161 ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc gaagtggcta
    20221 aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca ctctcctcca
    20281 tttcattggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
    20341 cgaatccagc taccccagta taccacaatc gtaagctctc gttagttcga gagctctttt
    20401 ttttgctttg ctaccttgtt ccttgaagat tctgctgaat taagatagag ttaagttaac
    20461 tacaagaatt ggagggatcc acatgaaatg gaaagaaaaa gtagacgaag cagctgaaaa
    20521 gttatatgat ttggttaaat cagaaaaata caatattgaa gtgaatattc cgaaaaaagg
    20581 cggaaaagcg gtccaggtaa aatcaaaacg cccaacgaat catacaaaaa aatggatggc
    20641 taaaaaccgc taataataac aaaagatcca taagtcggca ttttcatcga ttttagggtc
    20701 ttttgttatt ttctaggttg aacatatcta aaaaattgtt tctgacaata ggagtaggga
    20761 gaacgatgtg tgtctaaatc ctctgttgat ggatagaaac ctttatcttt ttcagttgtg
    20821 aatcaggtat ataccagtta taatagaaga aacgaaagga cgggtgtacg ttatgtcgaa
    20881 tcaattacct gtatatatac agattcatga ccaattaaaa aatgaaatcg aacaaggtat
    20941 atggaaagtc ggagaccgtc ttccttcaga aagagaatta gcagtgaaat tcaatgtcag
    21001 ccgtatgacg ttgagacaag caattcaaaa cttggcagat gaaggaatct tagaaagaaa
    21061 gatcggttcg ggtacgtatg ttgcgcgaga aaaagtacaa gaaaaaatgt ctggtgcaac
    21121 aagttttaca gaaattatgg aatcacaaaa tcgagtaccg tctagtcgga caatctctta
    21181 tttcttgact tcgccaagct caagtgaaat ggaaaaacta aacttgagta aagatgacac
    21241 gatcttgcga atggaacgta ttcgtttcgc agatgatgtc cctatttgtt ttgaagttgc
    21301 cagtgttcca caaaagctga tcgatggcta cagcaaagca gagattactg aatcgttgta
    21361 tcgcacgcta gaagaaaaag gcgggaaaaa gatcggtcca gcgaatcaaa cgattactgc
    21421 tgtgttggct tctgagaaaa ttgctgacta tttagaggta aaacgaggag atgcagttct
    21481 tcgtttgcgc cagatttctt attttgaaga cggtacgccg tttgagtatg tgcgtacgca
    21541 atacgtagga aatcgttttg agttttattt ggaaaaataa aaaacaggtg atccctgatt
    21601 ccttattaga aataaaagag aatcagaatc acctgttttt atatatgatc agttatcagt
    21661 tgctcgtgat aagaaacaga accctcttca aattggactt gtccgttcag cagctcaatg
    21721 atggattctt taaagtggtc agcacttttt tcatctacag cacagatgac gctaacttga
    21781 tcagtatatt ggatttcttt tataaaaaaa tgttggagtt caagataatt ctgtaatttt
    21841 cctagttgcg gatacgtgag atctcctgtt gcaaggtacc ttctacgatt ccaatgtcag
    21901 acaatgcttg agagaccgca tgagtgtatg ctcgtattaa tccgcctgtg ccgagtttcg
    21961 ttcctccaaa atatcgagtg actactgcag ctacattgat cagttgattt tttttgagga
    22021 cttccaacat cggtacgccc gccgttccac ttggttctcc atcatcgctg cttcgctgga
    22081 tttcattttt ttctccaatg atgaaggcgc tgcagttatg gttcgctttc caatgttctt
    22141 tttttatttc ttgaatgaaa ctttttgctt ggtcctctga ttctattcgt ctaagagaac
    22201 agataaagcg tgattttttg atctcaatct cactttgtcc attttctttt attgtacgat
    22261 agcgctcgat catcttgtca cctctttttt tcattttctt tattcagtat aaataaagtt
    22321 ctaggttgtt tcaacactgt aacggttgcg caattgctgt agtatgcgat aataaagaca
    22381 aaggcaggtg gatctgggga tgcaggaacg aatgaaaaag tacgatgcaa ttacgcatta
    22441 tttgaagaac aatggcggct ctcaagtaac gttgaccttt acccaatttg acgaactgct
    22501 gtttcccagt aacgggcttc ccaaaacagc aagggaaagt acagattggt gggcaaatga
    22561 ttataaacac cctgaaaaag gtgcctatgg ctggataaat gcaggctatg aagtagttgt
    22621 catcaatttg gacaaagaat atgtcgtgtt caataaatta gtgaaatcta gttggctatt
    22681 tgattaaata aaccgcgttt agaaaacgtt gacaaatttc tttcttttta tataatagag
    22741 taacagacgc gaagaaaacg tttgcatagt ttgttttaaa aaggagagaa aagtatggct
    22801 aaaaaaacaa ttatgcttgt atgttcggct ggtatgagca caagtttact agtaacaaaa
    22861 atgcaaaaag ctgctgaagc aaaaggaatc gactcggata ttttcgctgt gtccgcttct
    22921 gacgcagata acaatcttgc aaataaagac gtggatgtgt tgttattagg cccacaagtt
    22981 cgctttatga aagctgattt tgagaaacgt ctggaaccaa aaggcattcc tttagatgtc
    23041 atcaatatgg ctgattatgg tatgatgaat ggcgaaaaag tgttagatca agcaatcacg
    23101 ctcataggtg aataaataga acagtataac tatcgaacaa gcgatccaaa aaatagatcg
    23161 cttgtttttt cgtatatttt tttagggcga agaatatgaa tcatttttgg ggtagaaggc
    23221 ttctgacgag agatattgaa gcaatagact gtgaggaggt aaatttacca aattatgaga
    23281 agatcgcagc agtggaacgt ttgggcaact ggattctttg tcatcgctgt ggaatgagga
    23341 cgtccgtcat tgatggacag cttgcagatt acggttattt ttgtacacat tgtctatatt
    23401 tagggcgatg cgacactcaa caagatttat atttgtttga tcagcctgaa gcaaaacctc
    23461 gagaagtcgt ctttttttgg acaggacagc ttactgaaaa acaaacggaa atctcaaaga
    23521 gaatactttg ccattcagaa aaaagacatc atcttatttg ggctgtaaca ggtgcaggga
    23581 aaacagagat gctttatcct attttagtgg aaactttaaa agctggagga agagtagcga
    23641 tttgtacacc gcgagtcgat gtttgtaatg aattgttttt acgctatcgt tatgtttttc
    23701 cagaagaaaa aatcacgttg ctccatggga atacagcaac ggcttatacc tttacttctt
    23761 ttgttatctg tacgattcat cagctatacc gtttttatcg gtcctttgat ttaatagtga
    23821 tcgatgagat cgatgcgttt ccttataacg gggatgcagg attagctcat gctgtgcaaa
    23881 cagcgattaa accagacgga cgattcatct acttaagtgc cacacctgat aaaaagttac
    23941 taaaggaaat caagcagacg tttgacatcc acaaacttgc tttacgcttt catcggcgtt
    24001 tgcttcctga acccgtcttg gttttcatga ataattggcg acaaaaatgc aagaacaaga
    24061 agaagataaa atctttggtg cggatcattc atcaattaat taaagaaaac catatccttt
    24121 tattttgtcc aagtatcacg atgatgcatc gtttgaaagc cacacttcaa gaggctcttc
    24181 cgagatatga gatcacagat gtttcttcaa gagacaatca gcgggcggaa aaagtccaga
    24241 aaatgcgaga agcaaaatat gacatactgt taaccacgat gatccttgaa cgtggagtca
    24301 catttgaaag aatatccgtt atcgttttag gagcggatca tccggttttt actaaatctt
    24361 ctttggtaca aatcgcagga agagccgatc gtaaagggcc ttatacgaac agtcaagtat
    24421 actttttcta tgaagaaaaa actgcagcta ttcgaaaagt ttgtcaggaa atcaaagaaa
    24481 tgaatgaaga agggaagaag atgctgtgag gtgcagttgc tgcagtaaag atatcttcca
    24541 aaatttaaca ttcagcgaga ttattggtgt ttcgaaatac agattacctc agacttgttc
    24601 agtttgtcgc gcacaatttc atttattgga aacaagaggc tgttctggct gtcaaaaacc
    24661 aatccatttc aatctagaga gtacgaaaaa gaaagtaaag tctattcccg tttactgcag
    24721 tgactgtcaa gaatggaaga agaaatatcc gacctatgat tttcagcacc gtgccttttt
    24781 tgtgtacgat gaaaatatga aggcctggct caatcaatac aagtttatgg gagatatccg
    24841 actagcaggc acctttgcat atagatggac gcagataaaa aaagaatacc cgacatacat
    24901 ctattgtcca atacctttat ctgaaaaaag gcgaacagaa agaggattca atcaagtaag
    24961 cgagatgttg aaagcagcaa gaattccttt tcagatgcta ctcaaaagag agaagcatct
    25021 gctggctcaa gcaaaaaaca cgcgaaagga aagattggag atgcctcaac cttttgaact
    25081 tgcagtcacc ttttctaaga tacaaaataa aaaaatattg attattgatg atgtctatac
    25141 aacaggacag actcttttcc acgcagcaga ttgtttgcag gtcgcacagc cgaaaatcat
    25201 ccgaaccatt tctttggcga gatgaattag atgtggaaat ttgatagcga tttaatttgc
    25261 aaataaccta gagttcgata taatagaatt aagaagaggg aaagagtggt ccttctcctt
    25321 cttgattgtg gtagacgaaa ggggtaattt ttatgtttag atataatgta cgcggcgaaa
    25381 atatcgaagt aactgaagcg attcgcgact acgttgagaa aaaagtaggc aaactagaac
    25441 gctatttcag tgacgcaccc gatgcaacgg cgcatgtgaa cttaaaagtt tatacagaaa
    25501 aaacagcaaa agtggaagta acgatcccat taccttatct tgtattacgt gccgaagaaa
    25561 cttctccaga tttatacgca agtatcgatt tggttgttga taaattggaa cgccaaatcc
    25621 gcaaattcaa aacaaaaatt aatcgtaaat cccgtgaaac gggcatgaat actgcagatg
    25681 ctgctgcttt attcaatgaa gaggacgaaa acgaaaatga ttctgaatta gatatcgtac
    25741 gtacgaaacg cctttcattg aaaccaatgg atagtgaaga agcagtctta caaatgaaca
    25801 tgctgggaca taacttcttt atttttgaag atgcagaaac aaatggtaca agtatcgttt
    25861 accgccgtaa agatggaaaa tacggactga ttgaaacaga ctgatcagcc ttacttaaat
    25921 aacaacgaaa acttgcagca gctactgaca gctgccgcaa gtttttttga gtttcctcct
    25981 cttttagatg aaatatgaat aaattatgaa gttcttactt taatacgctt aagatatttc
    26041 aattatctat aactaatgat aaaatgaaaa gtaaagtcta catagtctgg ataatacaca
    26101 gaaggaaaag gagattacac aacgcatggc caatttgatt cgttcactta tcgaaaacga
    26161 taagaaagaa ctaaaacgtt tagatagcat tgcaaaaaaa gttgaagcat acgctgacca
    26221 aatggcagca ttaactgatg aacaattaca ggcaaaaact gctgaattca agggcagata
    26281 ccaaaaagga gaaacattag atcaattact tccggaagct tttgcagtcg tccgagaagc
    26341 agcaaaacgt gtattaggtt tatatccata ccacgtacaa ttgatgggtg gaatcgtgct
    26401 tcacgatgga aatatccctg aaatgcgtac tggtgaaggt aaaaccttga ccgcaacgat
    26461 gccagtatat ctaaatgctc tgactggcga aggggtacac gtagttaccg tcaatgaata
    26521 tttagcaacc cgtgactcga cggaaatggg cgaactttat aatttcttag gattaacagt
    26581 ggggttgaat atcaactcaa aatcttcgga tgaaaaacgc gaggcttata attgtgatat
    26641 cacttatagt acgaataatg aattaggatt tgactacttg agagacaata tggtcgttta
    26701 ccgccatcaa atggtacaac gtccgctgaa ttatgccatt gtcgatgaag ttgactcaat
    26761 cttgattgat gaagcgagaa cgccgctgat catttcaggg caagcagaaa aatccacagc
    26821 tttatataca agagcagaca acttcgtgaa acgtttgaaa gaagaagaag attataaaat
    26881 cgatgtacaa tcaaaaacaa tcagtttaac cgaagcaggg atcgaaaaag cagaagaaac
    26941 attcggtttg gataacttat acgacattga aaatactgca ttgacccatc acttggatca
    27001 agcattgaga gccaactata tcatgctttt agatatcgat tatgttgtcc aagacggaaa
    27061 agtactgatt gttgaccagt tcactggtcg gatcatggat ggacgccgtt attcagatgg
    27121 acttcaccaa gcaatcgaag ctaaagaagg cgtagagatc gaagacgaaa cgaaaaccat
    27181 ggcgacgatc actttccaga actatttccg tatgtacaag aaacttgcgg gaatgactgg
    27241 tacagctaaa acagaagaag aagaattcag agaaatttat aacatccaag tataccaaat
    27301 tccgacaaat cgtccggtta tccgtgatga tcgtccagat ttgctttacc caacattaca
    27361 aagcaagttc cacgctgtcg tgcaagatat caaagatcgc taccgcaaag gtcaacctgt
    27421 gttagtcgga actgttgcgg ttgaaacatc tgagttatta tcagatatgc tgaatcaaga
    27481 acgtattcct catgaagtgc tgaatgcgaa aaatcacttt aaagaagcag agatcatcat
    27541 gaatgctggt caaaaaggtg ctgtgacgat tgctaccaac atggctggtc ggggaacaga
    27601 tatcaagtta ggacttgggg taagagaact tggtggtcta gcagttatcg ggacagaacg
    27661 acatgaatct cgtcgtatcg ataaccagct tcgaggacgt gcaggacgtc aaggagaccc
    27721 tggtatgtca cagttctact tgtcattgga agatgatttg atgaaacgat tcggttcaga
    27781 acgaatcaaa gctttcttag atcggatgaa gatcggtgag gaagatgctg ttatccaaag
    27841 caagatgctg acgaaacaag tagaatctgc acaaaaacgt gtagaaggaa ataactatga
    27901 cacacgtaaa aacgtcttac aatacgatga tgtgatgcgt gaacaacgtg aagtaatcta
    27961 taaacagcga caagaagtaa tcatggaaga aaaaagctta tccaagccac taatgaacat
    28021 ggtcaaacgg acaatcagtc gtgtagtaga tgcgcataca caattagaga aagaaaattg
    28081 gaatttggaa agtatcatcg attttgccgg cagtactctt gtccatgaag atagcattac
    28141 tcttgcagat atcgaaggaa aaacagctca ggagatcaaa gagtatctaa acgaacgagc
    28201 acaagaagta tttaatacca aagcggctca actaaatggt ccagagcaac tgttggaatt
    28261 tgaaaaagtc gtgatcttgc gtgttgtgga ctctaaatgg acagaccata ttgatgcaat
    28321 ggatcaatta cgtcagtcaa tcggcttacg tgcttatgga cagaacaatc cattagtcga
    28381 atatcaaaca gaaggctata agatgtttga agatatggta ggcgccatcg agtatgaagt
    28441 cactcgaatc tttatgaaag ctgagatccg tcaaaacgta cagcgtgaac aggtttctca
    28501 aggaacagct tctcactcag aagacggaga tgccaaagaa aatagtaata ctagcgcgaa
    28561 aaaacagcca atccatgtga ataaggtcgg aagaaatgat ccttgtcctt gtggaagcgg
    28621 gaaaaaatat aagaactgtc atggaaaaaa tgcaaactaa tttctgacag caggattggt
    28681 gagacaaatt ctttttttaa gaattcgtct caccttcttt ctgttatttg ctgattctgt
    28741 tagactagat ataatgaaaa aagtatagtg aaagaaggaa aaaacatgga aatatccgaa
    28801 attcgaaatc tcctgacaga aatgcaggaa aaggtcacaa gtttcaggag gtctctttga
    28861 cttagatcag ctggaagaag acatcgcgga agcagaagca aaaatggctg aacctggttt
    28921 ttgggatgac agcgaagccg cacaaaaagt gatcagcgaa aataacgcta ataaagaaac
    28981 ctatgaccaa ttccattctc ttgccgaaga actagaagaa atcgaattga tggtggagat
    29041 gctgcaagaa gaacctgatg cagcaatgca agaagaagca gaaacacgta tcaatgcatt
    29101 agatgaaaaa atgaagacgt atgaattatc tatgctgtta gacggacctt atgatcgaaa
    29161 caatgcgatc gtcgaattgc atccaggggc tggcggaacg gagtcccaag actggggcag
    29221 catgctttta cgtatgtata cacgttgggc agagcagcat ggctatcaag tagaaacact
    29281 agattatcaa gcaggtgatg aagcagggat caagagtgtg accctgctga ttaaaggaca
    29341 taacgctttt ggttatctaa aatcagaaaa aggtgttcat cgccttgtac ggatttcgcc
    29401 ttttgactca gcgaaacgac gtcatacttc tttttgttcg gtagatgtga tgccagagct
    29461 tgatgaaacg atcgatatag aaatcaatcc agatgatttg aaaatcgata cgtaccgagc
    29521 aagtggtgca ggtggtcagc acatcaataa aacagaatct gctgtacgga tcactcactt
    29581 gcctacagga acagtagtag ctagtcaggc acaacgctca caattgaaaa accgtgagca
    29641 agcgatgggg atgttaaaag ctaagcttta tcaattagaa gtggaaaaga aagaacaaga
    29701 agcagctgca cttcgaggag aacaaaaaga aatcggctgg ggttcacaga tccgttctta
    29761 tgttttccat ccctattcta tggtaaaaga ccatcggaca aacgtagaga caggaaatgt
    29821 ccaagcagtt atggacggtg accttgattt atttattgac ggctatttaa aaatgcatct
    29881 acaataagaa cccattgtta aattgaaata aaattgtaag attttgcaag catgttgaaa
    29941 cattgccatg ctataatagc taaagactga aaaagatatg gagagaacgc catgattgaa
    30001 atgaaggatg taatgaagaa gtactcaaat ggtacgaccg ctatccgcaa tctctcgatt
    30061 gtcatcgaac aaggagagtt tgtctatgtg gttggtccgt ctggagccgg taaatctaca
    30121 ttcatcaaat tgatgtatcg agaagaaaaa gcttcaaaag gaactttgaa cgtcgcaggt
    30181 catgatctaa tagcaatcaa aaaccgcgaa gttccttatc tacgcagaga aatcggcgtg
    30241 gtgttccaag actataaatt attaccaaga aaaacagtat atgaaaatgt tgcgtacgct
    30301 atgcaagtta tcggcagaaa acctcgagat atcaaaaaac gagtaatgga agtgctggac
    30361 cttgttggtt tgaaacacaa agttcgagtc tttcctagcg aattatccgg tggggaacag
    30421 caacgtgtct ctattgctcg ggcaatcgtg aatacgccta aagtacttat cgcggatgaa
    30481 ccgacaggaa atctggatcc agaaaactca tgggaaatca tgaaattatt agatcggatc
    30541 aatgcccaag gaactactgt cgtgatggct acacataaca gtacgatcgt aaatacgatt
    30601 cggcaccgag taattgctat cgaaaacggc cgcattattc gagatcaagc ggaaggggaa
    30661 tatggatacg atgattagaa cctttttccg ccatttatta gaaagtatca agagtttgaa
    30721 acgtaacggc tggatgacag ttgcttcagt cagtgcggta acgatcacgt tgactttagt
    30781 cggtgtattc atggcagtta tcatgaatgc aactaaatta gctcaggata tagaaggaaa
    30841 cgttgatgta tctgtattcg tagatatcgg tacaaaagac gaagagatga aaaagctaga
    30901 aaaagaattg aatgacttag atcatgttaa aaaagttgaa ttttcaagca aagaacagga
    30961 attgaaaaaa atccaaaacg aaatgggtga ttcctggaaa ttgtttgaag gtgacaataa
    31021 cccgttatat gatgtgtatg tagtaagtgc tactgatcct tcttatacaa aacaagtagc
    31081 agaagaagca gcagatttga agaatgtttc tcgttctgat tacggtggag cttcctcaga
    31141 tcggattttc caaatcgctg gtgcagttcg gacatgggga ttagttgcag cggcattgct
    31201 gctgttcgta gcgatgtttc tcatttcgaa cacgattcgg atcacgattt tgtctagaca
    31261 gcgtgaaatt caaatcatgc gtttagtcgg tgcaaaaaat ggatatattc gctggccgtt
    31321 tttccttgaa ggtggctgga tcggtctgat cggtgcgatt cttccgattt tgatcatcac
    31381 atttggttat gcatggtttt tccaattgat caatccatca ttattacgtt cccattattc
    31441 attgattcat ccacaaaata tcgtttggaa aatcaatctt ttaatggtcg gcattggtgt
    31501 gatcattggt tcacttggct cgatcatttc tatgagacgt ttcttgaaat tctaatacca
    31561 caatataaat aaaatgagaa agttggacag tttcgttcca gctttctttt tttattggct
    31621 aggagaatag aaggaagcgg cttttttccc tttactttaa aaaacaactt gggtattctt
    31681 aagttggtag aagaagaggt gaagccgtga aaattatcgt tgacaatttt tttacgatcc
    31741 ttttgattat gaatattttg ctttccttta tcatcgtttt tcgagaaaga aaggaaactg
    31801 ctcaaacatg ggcatggctg ctcgttttga tgttcattcc agttgtagga tttattttat
    31861 atattttttt aggaagaggg atttccaaag agaaaatctt tgatttgaag atccaaggga
    31921 aaatcggtaa gaacctggaa attgaagaag acaaacaggc attgatgcga gggctttatc
    31981 ctcatccgcc tactggacaa gtagatgtga aacaattgat ctatatgctc acggtatttg
    32041 aaagtacgct ctatacgact aaaaatgaaa tcactttatt tacagatggt cgtgaaaaat
    32101 tcgatgcgct gattgaagat atcaagcagg cagaagacca tatccacttc cagtattata
    32161 tttatcgcag tgatgcatta ggtgaagaag ttcgagatgc attgacagag gcggcaagac
    32221 gaggggtaaa agtcagagtc cttcttgatg catggggttc cacacaagtt tcttccagct
    32281 tttttcagaa tttgaaaaaa gcaggcggag aagtcgcatt tttctttcct ctatttgttc
    32341 cgtatattaa tcctcggatc aattatcgca atcatcgtaa aatcgtagtt attgatggga
    32401 aaatcggata tacaggtggg ttcaatgttg gaaatgaata tctaggattg gtaaagaaat
    32461 ttggttattg gcgagacaat catttgcgta tttacggaga agcggtgtat accttgcaaa
    32521 accgtttttt gatggactgg aattctcagc atacaaaaga agtaggctat tctcccaaat
    32581 tttttccgtc cattcacact actggtgata tagctgttca aatcgtgaca agcggaccag
    32641 ataccgaaca tgaacaaatc aaaatgactt atttgaaaat gatttcgtta gccaaacggg
    32701 agatattgat ccaaacgcct tattacatac cggatggatc gattcatgaa gcactgaagt
    32761 tggctcttct ttcaggcgta aaagttcata tccaaatacc taacaaacca gatcatcttc
    32821 tcgtttactg ggcaacgtat tctttcgctg cagagctgat cgagtatgga gcaaggatcg
    32881 aaacatatga gaatggattt attcatgcaa agacgatgat tatcgatggt ggcattgttt
    32941 cagttggctc agcaaatatc gatgttcgtt catttcgctt ggactttgag gtcaatacac
    33001 tggtttatga tgaacgaatg gcttcacgtg taagaaaagc tttttttgaa gactcgaaaa
    33061 tttctacgca tctcacaaaa gaaatgtatg aaaaccgagg aataatcatc aagatgaaag
    33121 aaggattagc aagattgatt tcgccgctcc tttaaaacat aaaaaggaca tgagaaccat
    33181 caacagatgg agatctcgtg tccttttcta acggggccat ttctgagagt acgtcagaaa
    33241 tggagaaagc gtctcgcatg agcgaaatta actttggggt atctcttgtt ttttggggaa
    33301 agcaagaaat aagctaatat atgctattaa aagagacaca tttcaaagcc agtatagcat
    33361 agaaactttt aatgagatag ttttttttac aattatgaga atttattttg tt
//
LOCUS       DAMYXM010000025        29268 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.026, whole genome shotgun sequence.
ACCESSION   DAMYXM010000025 DAMYXM010000000
VERSION     DAMYXM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.026"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(140..1189)
                     /locus_tag="PNS21_001372"
     CDS             complement(140..1189)
                     /locus_tag="PNS21_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5159086.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFTRGAS"
     gene            complement(1271..1960)
                     /locus_tag="PNS21_001373"
     CDS             complement(1271..1960)
                     /locus_tag="PNS21_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDI5159087.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(2066..2434)
                     /gene="rplL"
                     /locus_tag="PNS21_001374"
     CDS             complement(2066..2434)
                     /gene="rplL"
                     /locus_tag="PNS21_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDI5159088.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2487..2990)
                     /locus_tag="PNS21_001375"
     CDS             complement(2487..2990)
                     /locus_tag="PNS21_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDI5159089.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3194..3321)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3400..4089)
                     /gene="rplA"
                     /locus_tag="PNS21_001376"
     CDS             complement(3400..4089)
                     /gene="rplA"
                     /locus_tag="PNS21_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDI5159090.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4193..4615)
                     /gene="rplK"
                     /locus_tag="PNS21_001377"
     CDS             complement(4193..4615)
                     /gene="rplK"
                     /locus_tag="PNS21_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDI5159091.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4958..5626
                     /gene="sdaAB"
                     /locus_tag="PNS21_001378"
     CDS             4958..5626
                     /gene="sdaAB"
                     /locus_tag="PNS21_001378"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDI5159092.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5639..6511
                     /gene="sdaAA"
                     /locus_tag="PNS21_001379"
     CDS             5639..6511
                     /gene="sdaAA"
                     /locus_tag="PNS21_001379"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDI5159093.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6602..7621)
                     /locus_tag="PNS21_001380"
     CDS             complement(6602..7621)
                     /locus_tag="PNS21_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDI5159094.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7794..8699)
                     /locus_tag="PNS21_001381"
     CDS             complement(7794..8699)
                     /locus_tag="PNS21_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDI5159095.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8758..9303)
                     /gene="nusG"
                     /locus_tag="PNS21_001382"
     CDS             complement(8758..9303)
                     /gene="nusG"
                     /locus_tag="PNS21_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDI5159096.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9404..9574)
                     /gene="secE"
                     /locus_tag="PNS21_001383"
     CDS             complement(9404..9574)
                     /gene="secE"
                     /locus_tag="PNS21_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDI5159097.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9598..9750)
                     /gene="rpmG"
                     /locus_tag="PNS21_001384"
     CDS             complement(9598..9750)
                     /gene="rpmG"
                     /locus_tag="PNS21_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDI5159098.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9912..10556
                     /locus_tag="PNS21_001385"
     CDS             9912..10556
                     /locus_tag="PNS21_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDI5159099.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10612..11115)
                     /locus_tag="PNS21_001386"
     CDS             complement(10612..11115)
                     /locus_tag="PNS21_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDI5159100.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(11128..11174)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11712..12677
                     /locus_tag="PNS21_001387"
     CDS             11712..12677
                     /locus_tag="PNS21_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDI5159101.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12776..13948)
                     /locus_tag="PNS21_001388"
     CDS             complement(12776..13948)
                     /locus_tag="PNS21_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5159102.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13935..15080)
                     /locus_tag="PNS21_001389"
     CDS             complement(13935..15080)
                     /locus_tag="PNS21_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5159103.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(15094..16026)
                     /locus_tag="PNS21_001390"
     CDS             complement(15094..16026)
                     /locus_tag="PNS21_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5159104.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            16143..17222
                     /locus_tag="PNS21_001391"
     CDS             16143..17222
                     /locus_tag="PNS21_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDI5159105.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17219..17848
                     /locus_tag="PNS21_001392"
     CDS             17219..17848
                     /locus_tag="PNS21_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI5159106.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17958..18185)
                     /locus_tag="PNS21_001393"
     CDS             complement(17958..18185)
                     /locus_tag="PNS21_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDI5159107.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18288..20876)
                     /locus_tag="PNS21_001394"
     CDS             complement(18288..20876)
                     /locus_tag="PNS21_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDI5159108.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20896..21555)
                     /locus_tag="PNS21_001395"
     CDS             complement(20896..21555)
                     /locus_tag="PNS21_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDI5159109.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21604..22107)
                     /gene="trmL"
                     /locus_tag="PNS21_001396"
     CDS             complement(21604..22107)
                     /gene="trmL"
                     /locus_tag="PNS21_001396"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDI5159110.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22328..23194)
                     /locus_tag="PNS21_001397"
     CDS             complement(22328..23194)
                     /locus_tag="PNS21_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDI5159111.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23303..23944
                     /locus_tag="PNS21_001398"
     CDS             23303..23944
                     /locus_tag="PNS21_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDI5159112.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(24006..25367)
                     /gene="mgtE"
                     /locus_tag="PNS21_001399"
     CDS             complement(24006..25367)
                     /gene="mgtE"
                     /locus_tag="PNS21_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDI5159113.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25385..26284)
                     /locus_tag="PNS21_001400"
     CDS             complement(25385..26284)
                     /locus_tag="PNS21_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDI5159114.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26286..27083)
                     /locus_tag="PNS21_001401"
     CDS             complement(26286..27083)
                     /locus_tag="PNS21_001401"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDI5159115.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(27061..27765)
                     /locus_tag="PNS21_001402"
     CDS             complement(27061..27765)
                     /locus_tag="PNS21_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDI5159116.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27896..28471
                     /locus_tag="PNS21_001403"
     CDS             27896..28471
                     /locus_tag="PNS21_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDI5159117.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28589..29266
                     /locus_tag="PNS21_001404"
     CDS             28589..29266
                     /locus_tag="PNS21_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDI5159118.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8612 a         6192 c         5259 g         9205 t
ORIGIN      
        1 ttataaaagt atctgtataa tgttcagtat attttgatga acgcatatgt agtcctcatt
       61 tcttgggagt tttgagacta ctatatgcgt ttttttgttg atttaccaac atgtttagac
      121 attttcctct aggaaaaaat taactagcac cacgggtaaa tacagtatta tttcggttgc
      181 ttgctgcttc acctagccct tgtactcgtg tttgattggc gacttgacca aattgattcc
      241 cgacgtctaa taaaataata gctaaaagca aaatggccaa atggcttccg ccaaccaata
      301 aagcaataaa gcttattgtt tgcataacaa gtcccatata cacgattttt ctttcagaat
      361 aagaatctgc cagtcgtccc acgataggtg cagcaactgc tccagacaaa ccaaaaatcg
      421 ctaatatccc tgcttcgaat gttccccaat tgtaggcggg actgctgata taaaaaatca
      481 acgtagacca aaagatcgaa aatgttccaa acatgaaaaa tccactgact gccgcttctc
      541 ttaatagacg ttgagaagca actaaatgcg gcaaactttt aagtgaatca aggtatgaaa
      601 cagctgtact actttgctgg acagaacgcg gcattttcca gtaaagcaga agtgtaagaa
      661 ttccgactgc acataaagca aataaataga tcatccgcca agaaaaaaga ctggcaacaa
      721 taccagatac cgtccgagaa agcaaaatcc ccacgagtaa accgctaagc aatgtaccca
      781 tgaccttccc tcttgctttt ggtcccgcta gtaccgcacc gtaaggaatg atgatttgcg
      841 gcacgatcga aagtaatcct attaaaaaag aagcgcatgc aaataacgta aacgaaggcg
      901 caaaaaaagc ggccaacaga gagacggcag ataacgctac cattcgaatg atcaattttg
      961 gacgatccac cacgtcccct aaaggaacta aaaacagtaa ccctaacgca tagcctaatt
     1021 gggtcaacat cgtcaatatc ccgactgcac ttgtgctaac agataaacta gctgctactt
     1081 ttgttccaat cggctggatg taatacatat tggcaacaac aacgccacaa gtgattgata
     1141 atagtaaaat aagcgaagtt gttaaaggtg ttttttcatt actcttcatt gatttccccc
     1201 atttcctatc ttagtttaca acttatggaa aagattggaa atagaaaaat acaactattc
     1261 gccacactta ttatttgtgc ttttgttttc cttgtttttt cttgcgatct ttataaatga
     1321 agacaactaa ccctactagc acagtaacac ttgcaaccaa cgtgactgca gttttgttat
     1381 atccgtgaaa cagagcttct ccaccacacg catacaacac agacgaaggg atgactccaa
     1441 tcacgatcga caatatgatt tgctgtagag gcaatttcat cgcatctgca gcaaaattga
     1501 ccatagaaga tggaataacc ggtatcatat agcctaacat tacacctatt tttggatgtt
     1561 tcatttgtcg gattgcttgc acccaatgat tcgtttcggt tttcttatct aaaaatttta
     1621 agcgatgcat aaggaaaatt gcagtgatgt taccaagagc attacccaaa atgttgatga
     1681 tcgcacctgt cagtggtcca aagctcaaac cagcaatgac tccaactact gaagtaggaa
     1741 tacctggaac agcacacatc accgctgtca atacgatcaa gagtatcgat gcaaaagtcc
     1801 catgcgaacg aatatgtgcg agtagctctg ccttactcgc tctcggattt aaaaataatt
     1861 ggatgtctgt ctgatattct aaatacaacc gatagcccac taccagtatc agaatgattc
     1921 ccaacgagat caacatcaat cggattattt tctttttcat atttcctcct gaaaaaaatc
     1981 tatattcatt aaataatagt ttaacataaa aacaaaaaag caccgaaatc caacatagga
     2041 tctcagtgct tttaaatagt aaaaattatt taacagttac agatgcgcca acttcttcaa
     2101 gtttagcttt caattcttca gcttcttctt tagatacgcc ttctttaact ggtgcaggag
     2161 cgccatcaac tacagcttta gcttctttca agcctaaacc agttgcttca cgaactgctt
     2221 tgataacttt aactttttgg tctccagctg cagttaattc tacagtgaat tctgtttgtt
     2281 cttcagcagc agcagcgcca ccagcagcag ctacagctac aggagcagca gcagatacgc
     2341 caaattcttc ttcaatagct ttaactaggt cgttaagttc taggatagtt gcttctttta
     2401 actcagcaac gatgttttca atgttcaatg ccattataat ttcctccgtt taattgttct
     2461 tttttattta atgctttatg cggcaattaa gctgcatctc catcttcttt gtctgccact
     2521 gctttgacag cgtaagccac gttgcggact ggcgcttgta gtacagatag caacatagat
     2581 agaagtcctt cgcgatttgg caattttgcc aatgctgtga tttcttccaa tgaagagact
     2641 ttaccttcga tgataccgcc tttgatttct aatgcttttg cttctttagc aaattcgtcc
     2701 atgattttcg ctggtgcgac tacatcttcg ttactgaatg caacagctgt aggtccagtg
     2761 aatacttcgt ccatgccttc aaggccagct tttttagctg cacgtgaaag aatagaattt
     2821 ttgataactt tcatttctac gttagcatca cgaagttgtt tacgtaaacg tgttacttca
     2881 tcaactgtta atccgcggta gtcaacgact actactgatg cagctgctgt aaatttttcg
     2941 cttacttcat caacgattgc tgcttttttt gcgataattg cttcactcat ttttgagttt
     3001 cacctcctga attttgatgg aagagttttt tgcactgaaa aggcaagtca cttttatagt
     3061 tactttgctc aaccagtatt attcatcacc tattcacttc gttcatagtg attctactcg
     3121 ctttgctcga aaggaaactc ctttcgcttt gctcaaccag tattgttcat catctcttca
     3181 cttcgttcat agtgattcac aataaaaaac tctatgccac cgcagacata gagggactat
     3241 tgaatatatt caataaacgt cctcggtagg aaattaaggc ttgatgccac ctactgtctt
     3301 cggtacaagt taattattaa cctcaactag cttagcatgc taaactagtt gagtcaagaa
     3361 ttatttttca gttggatcaa tcaacattct aatatttaat tagaaagaag cttggtcaac
     3421 atggatacca ggtccaaatg ttgttgtgat cgttacattt ttcatgtatt gacctttagc
     3481 tgctgatggt ttagctttta gcaatacatc atggattgct gcaaagtttt caactaattt
     3541 ttcatcgtca aatgaaactt ttccgattgg tacatggata tttccagctt tgtccacacg
     3601 gtaagttact ttaccagctt tcacttcgtt tactgctttt gttacgtcca ttgtaactgt
     3661 accagtttta gggtttggca ttaagccttt tggtcctaat actcgtccta gacgacctac
     3721 agtagccatc atatcaggtg ttgcaacaac tacatcaaag tcaaaccatc cgttttggat
     3781 tttttgaacc atgtcgtcgt cacctacgaa atctgcacca gctgcttcag cttcttttgc
     3841 tttgtctcct ttagcaaaaa ctaaaacagt ttgtgtttta ccagttccgt ttggtaatac
     3901 aacagcacca cggatttgtt ggtctgcttt tttagggtct acgttcaaac ggtatgcaac
     3961 ttcaactgtt gcatcaaatt ttgcaatgtt tgtttctttt gctaaagcta ctgcttcggc
     4021 aacaggataa actttacttg aatcaacttt ttttaatgca tcttgcattt ttttgctctt
     4081 tttagccatt ttggttcctc cttgattgtg gttataacgg ttgtacctcc cacgtgttct
     4141 atatctttca atatagagcc aactgagaag ctgctgtctt agggtgtata gattattcta
     4201 cagtgatccc catgcttcgt gcagttcctt caaccatgcg cattgctgct tcaacgttag
     4261 ctgcgtttag gtcttccatt tttaattcag cgatttctct tacttgatcg ctagaaactt
     4321 tcgcaacttt atttttgttt ggttcgcctg aacctttttc gattttagct gcttttttca
     4381 atagaacagc agctggcggt gtttttgtaa cgaatgtgaa tgaacggtct tcgtatacag
     4441 aaataacaac cggaatgatc aaacctgctt gatcagctgt acgagcgttg aattcttttg
     4501 tgaatcccat gatattgata cccgcttgac ctagtgcagg acctactggt ggagctggag
     4561 ttgctttacc tgcaggaatt tgcaatttaa cgattttttc tactttcttt gccacgagac
     4621 atacctcctt aagtccgtga tgtggttaat tggagatgca tatttctcct cccacgtttt
     4681 atctgatctg aaaaaatcca gacaaataat gtgcaaaaca atgcacactg gtacagtata
     4741 gcaaatttat ttgttttttc aagatttttt tctgctattt ttacattttg cttgaataaa
     4801 cgctaaattt attctaaaaa aacaacaatt tttcgttctc atttcttctt ttatgttaca
     4861 cttgcgctag catggttcaa cagatgaaag caaacaataa cacggttttt tagacccttg
     4921 tacaaagatt cctacataac aaaatgaggt ggagaaaatg aaagatttaa ggtatagaag
     4981 tgtctttgat attattggac ctgtgatgat tggtccaagt agctctcaca ctgccggagc
     5041 tgctagaatc gggaaaatca tacgtagtat atttggcgaa cagcctgatt ctgtagatat
     5101 ttatctatat gaatcatttg caaaaaccta tcgggggcac ggaacagata ttgcattagt
     5161 tggcggcctg ttagggatgg aacccgatga tgaacgttta gctgattctt tgaaattggc
     5221 atatgaagcc ggtatggaag tcttgtttgt tcctaagagt gaaaaagccg aacatccaaa
     5281 ctctgtaaag atgcttctaa aaaaaggaac acgaaagctt tcagtcactg gcatttcgat
     5341 cggcggtggc aatatccaga tttctgaatt gaatggattc aaaatttctt taagcatggg
     5401 tactcctacc ttgatcgttg tccaccaaga tgttccaggt atgattgcta aagtgactag
     5461 tatactttct gagaccaata cgaatatcgg cactatgaca gtgacacggg aatcaaaagg
     5521 tgaaaatgcc attatgataa ttgaagtaga tgatcctcaa gtagaagaaa cggtaaaaag
     5581 actcaaacag ctgccaaata tcgacagcgt aaattatttc gattaaggag ctaaaaaaat
     5641 gtttttatcg attgaagaat tagtagatca agcaaaagct tatccaagtg tagcagaatt
     5701 gatgattgca gttgaaatgg aaatgagtgg ccgttcccgt agtcacatca tcgagatcat
     5761 ggaaaaaaac ctctcagtaa tggaacaatc aatcaacgag ggaaaagaag gtgttacctc
     5821 cgtcacagga attaccggtg gtgatgcaaa aaaattagat gattatttaa atgaggcaga
     5881 ctttttaagt ggagaaacaa tcttaaaagc tgtacgcaat gctgtagctg ttaatgaagt
     5941 caatgcaaaa atggggttga tttgtgctac accgactgcc ggaagcgcgg gtgttgttcc
     6001 tggtgtgctg atggctgtcc gagatcggct tgatttgaca catgaacagc aattggaatt
     6061 cttatttacc gctggcgctt tcgggttagt catcgcaaac aacgcttcta tcagtggtgc
     6121 agaaggggga tgtcaagcag aagttggttc tgcaagtgca atggcagcag cagctttggt
     6181 ttgtgcaaaa aatgggactc ctcatcaagc agcacaagca gtagccatta cattgaaaaa
     6241 tatgatggga ctgatttgcg atcccgttgc aggcttagtt gaagtcccgt gtgtcaaacg
     6301 taatgcttta ggctcttctc aagcttttat ctctgctgat atggcattag cgggtatcga
     6361 aagtgtcatt ccgccggatg aagtcgttgc tgcaatgtac caagtcggac gtcaaatgcc
     6421 gtcgattttc aaagaaacag cagaaggtgg cttagctgtt acgccaactg ctaaacgact
     6481 tgcggaagag attttaaata agagggcgtg ataaagctcc ttaatttcga gtattaagag
     6541 attagttatc ttcataacaa aaaacgaaga gaggactaat tgcccttctc ttcgtttttt
     6601 attaatccat caatttcgta ttttttactt catcgatcgt ttttgttcct gcaagttgca
     6661 tcgtgatcga caattctttg ttcaggtgtt caaaaacaga tgttactcct tctgccccac
     6721 ctagattcaa cccataaatc actggtcggc caatcgcaac taagtcagca ccacttgcca
     6781 gagctttgaa gacatgctct cctcgacgga caccagagtc gaaaatcact ggtacacgtt
     6841 tgttgactac ttcggcaatt tttggcaata cttcaaagga cgcaggacct ccatctaatt
     6901 gacgtccacc atgattggat acccagatac catctgcccc tgcagagata gcgattactg
     6961 catcttctgg cgattgtatt ccttttacga taactggaag atgggtgatt tctttgattg
     7021 ttttgatgtc atctggtgtc aagccttgtt ttgcagctgc ataaatttct gcgattcctt
     7081 ttccttcgcc attaccactt gcactttgtt cgctatacgc agctaagtta ggcattggta
     7141 atggaaattg gaattgattg atgacgtctt cttcacggta accaccgagc gttgaatctg
     7201 ccgttaaaat gatagcttta gcccctgctt ctactgcttt gttcaaaatg aactcattga
     7261 atctgtcgtc tttactcata tataattgga aaaactgcgg tgcgcctggc gctgcatcgg
     7321 ctgcgtcttt gattgtcgta tttgcataag tgctgatgga aaagattgat ccagcagcag
     7381 ctactccttt agctgtatca gcttcccctt tttcatgtgc caatccttgg gccgctgatg
     7441 gcgcttggat aatcggtgta tctaattcaa ttccgaaaac agatgtatgc aaatcggctg
     7501 agtcgattcc gcgtaatatc cgtggcatga ttttctttgt attaaaagaa gtcgtatttt
     7561 ctttcatcgt ccactcatct tcagaaccac cgcgaatata gccaaaagct cctgcttcca
     7621 tgcgctcttt tacacgacct tctagttagg caatgtttac aatatcgatt ggatgttctt
     7681 cattacttgt atgataagtc atagtagatc cttctttcat cttcttgatt acatataaat
     7741 catactaaac ttacaaaaag taagcaaaca tttactctca gatactttta tcgctattct
     7801 ttccaaataa atcgacttac aagctgatct acttcttggt tttcatgaag catactatga
     7861 gaggcatttt cacctgtcac tactttttcc tctatttcat ttccgcgttg cttcaacaac
     7921 gcatagaccg ctaatgcact gtttaacggt actgttccat cactcaagtc tgtttcatct
     7981 agttgaccag caatcaagaa aaatcgagtg gttataggaa tattaccgat catttgctgg
     8041 taatcttgat agcggttaga aacgacagca ggcccctttg ctaactcatc tgtcagagac
     8101 tgcgcactat catcgacaaa atcattaaaa ggcgcgccaa ttgctgttaa tgtctgaact
     8161 tgtggcaatg acgtatcctg tccatacgtt cctaaatagc gtagtgcact aactcctccc
     8221 atggaatgcc ccactatgtt gactttatct atgccgtagt tttctttcag atacaaaaga
     8281 caagttttga tccattctgc ttgattccac tcgttatttt tattatcttc aaacaatact
     8341 tggaccatcg gattgtctgc ttttccagac aaatctcctg ttgcttgtat ttgtccatct
     8401 gttcctactg ttaaaaccaa ttcttttact gcttgctgat ttttttccaa acgccgcaac
     8461 atccctttaa aggaattttc tgtcccgctg tagccgtgga aaaaaagtgt tggaatagtg
     8521 gttttttcta tcactttact ggattctgcc gttttttctt tatcagaaac ttcagtagta
     8581 tttatcttcg tcgtctggca accacttaac gcaaacaaac tgataaggac taaagctgct
     8641 gcagttgcac gtttgcataa atttttgatt ggttctgtca tacacctctt cctttccata
     8701 tagaaaaaag aaagacttga aaggccaatc atatgatcga catttcaagt ctctttatta
     8761 atcgatttta tctacttgat cgaaatcaag ttctgtactg gtttcacgtc caaacatatc
     8821 gatgttgact ttcaattttt ctttttctcc gtcaacttct gttacttgtc cttctaatcc
     8881 agcaaatgcg ccttcgatga ttttcacgac ttcgccaact tctacttcta gatcattttg
     8941 tcgcaggctc ataccgatag aacgcaagat atgattgact tcttctggaa gtaatggtgc
     9001 tggtttgctt ccggctccgt gtgaaccaac gaatcctgtc acaccaggtg tgttgcggac
     9061 aacataccat gaagcgtctg tcatgatcat ttcaacaagt acgtatcctg ggaatgtttt
     9121 atgaacgatt tctttttctt tcccattttt gacttcagtt tctttttctt caggaacaac
     9181 gacacggaaa ataaagtcgg ccattttcat actttgcgca cgagattcga tatttgcttt
     9241 tactttgttt tcatatcctg aatatgtatg caagacatac cagttttttt caaaagattc
     9301 cattgaaggc tcctcatttc attgtaaaat aaaaaaacct cagtacaccc gaagtttttc
     9361 tttcgcttca ttatagcact aaaagtacgt tgtggctagc gagttatttt aaaatccagc
     9421 caaataagct ttggatggct gtatccatta cgtaaaacat caatccgaaa ataatagaag
     9481 tttcgatgac tactaacgta tctttacgga gttgttttct gcttggccaa gttacttgtt
     9541 tgatttcatc ttttacgctt tttagaaatt tcatgagaca acctcccttg ctactgttta
     9601 tttcgtttct ttatgtaatg tatattgatt acaatattta caaaacttat tgatttccaa
     9661 acgttcgccg ctttttcctt cactgactga ttttgtgtag ttgcgagacc cacaaacgga
     9721 acaagcgagt gctgcttttt tctttgccat ttacttctgc ccccttgctg agaatcagac
     9781 ttatacttca taacagtatc acgtaacttt ttacttgtca atttgtgaaa taaactatct
     9841 attgttttgg ataaaattat gatatgatta atttgaaatt aagctaaaat gagacaattg
     9901 gaggtatagc gatgctatta aaaacacttg tatataagaa aaaagatctg acaaccgtta
     9961 cagagactag tacattagaa gaagcattaa aaattttaga aacttccgga taccgttgtg
    10021 tgccgatctt agattcttca ggtaatattt tccgtggaaa catttacaaa atgcatatct
    10081 atcgtcataa agcaaatggc ggtgatatga cgcttcctgt cactcatctt ttgaaaaacg
    10141 ctacaaagtt catttacctg gatacttctt tcttcaaagt attctttacg atcaaagagc
    10201 ttccttatat tgctgtcctt gacagtgaaa atcattttta tggtattttg acacatagta
    10261 cgttgcttaa tatgctctct caatcttgga gcgtagaaca aggaagctat gtactgacaa
    10321 ttgcttccac aggaaagcaa ggagatttgg ctgctattgc aaaaatcatt tcaaaacaca
    10381 gcagcattgc cagctgtatt actcttgata tcgaaaaaga cgaatacatt cgacgtacat
    10441 tgatcacgct accggctaac actgaagcga aagtatgtga aacgatcgtc aaccacttag
    10501 agaaaaagaa ttataaagtg gtagaaattg aaaatttgca aaaccaagaa gcataatcaa
    10561 aaagcgaaaa aagaaaacag ctaatctgct ttcttttttc gctttttata attagacacg
    10621 cttttcaaaa tcaagcgcgc gatctaaata atacatcaat ggtgctgaaa cagccatgac
    10681 gataaactca cttaatgcaa gcgtgccata ggttgtccaa aatgcttggc cacctgttgg
    10741 aactaacata taagcaatca gtcccatgct gatggtaaag aagatcgtgt tcaatactaa
    10801 acgcatcttt acatttttca ctttgttttg caataaagct gtcaggccta aagaaagcaa
    10861 tgtttggccg ccaccaaaaa ccacatccat gataccaaaa ccaaagaagg cattatagac
    10921 gatgaccccg cctaaaactc cccaaagcaa tttacggtta aagacaacta aatgattcaa
    10981 actttctgaa atacgaaact ggatcggccc tgaggataca ggcgcaacta aaatagttaa
    11041 cgctagataa agagccatga tgatgccatt tacaacgatt acggataacc gttgtctgct
    11101 tttcgttttt tgcattttct ctcctctttt ctcctagttt ttttaacgcg ggatggttga
    11161 tgaaccgcgg ttatgtgaaa caaacaataa ttataacaga cttttcattt atttcaatcc
    11221 gtcttatctg cttttaaaaa agcaaaagct taaacaatgt aaacgtttgc taaaaatcgt
    11281 gggatatggt ataaagtaag tgtaaggggt cataagaatt cccagtaaag cagacgaagt
    11341 aggatctgca agaacagaat tgttgtgaaa cttaaataaa taaaaaagga tgtgggaatt
    11401 tgagcaacct cttactagag aacggtctag caagcagctg aagatctaag tggacatgcc
    11461 gaaccttgga acttctcctt acgtaagtaa tggatcatgc gcttgctagg cttttttgtg
    11521 tttttcggtc ttcggacctt tttcattttc ctagacttct gctataataa ccaagaatta
    11581 attttgtaac agattttttt gaaaaaacaa gtttctttca ttcaaaaaat agaaaaagct
    11641 tgctaattat tggttaacca gcaaacagga gtattaaaat atcttgtttt attgaatagc
    11701 aggaggaaat catgaaattc cattatcata tattgatcaa tccatcggca ggcagcggaa
    11761 atggctataa agtagcagaa agaattttac cggtattaaa aaataagcat attgactata
    11821 cacttcatta tagtgaatac aaaggacacg atgctgagat tgctgaaact ttggcaaaag
    11881 aaacattgat tccttggata gaagaagaac agaaaacaat cgatactttc ccccttttga
    11941 tcatcgtagg aggtgacggc accttacatc aggtacttga tactttctat cagatggaag
    12001 tagaattccc agttgcttat attccagcag gttctggaaa tgattttgct cgaggggcag
    12061 gccttccaaa aaatcccaaa aaaggacttc agctgatttt agcagctcaa tcaccagaaa
    12121 aagtccacat aatggcttat gaagaaaaaa taagtgaaaa aaaaggaata gctgttaata
    12181 atttcggtat aggattagat gcagctatcg tccatgcgac aaatcattct tccacaaaaa
    12241 agcgattgaa caaatacaac ttaggatcat tttcgtattt attctctatt ctccgtgctt
    12301 tattcactca aaaaggattt ccaattctgg tagatgcagg aggaaaacaa ttaagttttt
    12361 caaacgcatt tttatgtaca gcaacgaagc atcctttttt tggcggagga atcgcgattg
    12421 ttccaactgc tgatgcgtct aaaccagtca ttgattttgt cgtggtcgaa cgaatcaatt
    12481 tctttaaaat cttgtggctc attcttttac ttatacagaa aaaacaattg aaatcgaaat
    12541 atttccatca ctacacaaca aacagattac gtatcgtttc aacaacaccg caacatggtc
    12601 aagaagatgg agaagaaatg gaaaaacgac cttttgatat cacaataacc acaactaaac
    12661 agttgttttg gtgttaataa aagaggtttt gtcagaagct acatctggaa caccgtttat
    12721 tcggaaaaaa ggactgtgac acgacttttg tcacagtcct ttttttacta tgcagttaat
    12781 tctttgccca attgggttgc catcgatagt ttctttcttt acccaaaata aattggatca
    12841 ttgaaaacac cgcagcaaaa gcaactaaga cacaaccatt gaaccaaaaa gattctgcaa
    12901 atgtttgatt gtaactgagt gtttcactga tcaagttatt attttgaaca aaccaatatg
    12961 ttggtgtgaa tgccgctatt ttgctaacca tttctggcaa gtattctgct ggtacaaaca
    13021 ccccaccgat aaagcaactg cctgtgataa aaatatcttt gatcccaccg ctggtttctg
    13081 agttttttac caatgaagtg atacaagccg aaaagctaat gatcggcaat aaaaacaaga
    13141 aagtatttaa aatatgatac cataccgtta tatcccacga ggtgtggatc actaacagaa
    13201 ccattagaag taagaagcca ctgaacaaca gcagcgagta actaatcaaa ctaaaggtta
    13261 ctttgcgatt caagtttcgt ttggtaatgg gcgcactctg gtttcgttta ctgatctctt
    13321 tgcgattaaa cgctagattg atgacgctga aaccgctaaa aatggataga aacaacccat
    13381 aagacaagaa attaaaggtt gtgcccgtca acagcttttt catccgctga ctatatcccg
    13441 cacttagctc aacttctcca gtaaccgaga gtgccatttg tgtctgttcc atagctgcat
    13501 cgattgtttt atcttcttct tgaaacagaa tcagtgtttg caaaaaggtg ttcacttgct
    13561 gtgtaacaac tgttttagaa aaggcatctg ggcgactctg tatcttgatc gtcgccatct
    13621 ttcctacttc gacttgttcg gtaaagtctg caggcagatg taagataatg tttacacgat
    13681 taaaataaag agcatcatcg atttcttttt gtgaggttcc atccaatgta acgactgttt
    13741 ccttgtcatt caaatattta cgaaggccat cgactacagg cgactcttct tcactaatca
    13801 aggcgatttt cgcatcttgc agagaggtat tttcaacatc aatattttgc gcaaaaaaga
    13861 atgtggcaac aacagtgata attacaccta tcagtaaatt cgatttattt gctttaagca
    13921 atgtccaaat cgttttataa agcgtcataa cgcacccctc tttccatcca agcatttcct
    13981 aacgcaaaaa ggaaaataaa tccgatcaac catagaagat tggtataaaa actagccaaa
    14041 tcagtgtaga aataaagttt atacaaactt tcgctgatca agttgacgag atttaattga
    14101 ccaacaatcg gaatatgaag atcaatccaa tacttcactt gttccgaccc catcatacca
    14161 gcaaaaaagc tcatcgcaat cgtcactgaa atcaagatgc tatccttttg caccagattc
    14221 atttttggta tcaaattacc aaccaatgta cctaacaaaa tcgcacacaa cgaccccaga
    14281 gcggtaacaa tcatcaacca ttgccaacgt tcaccaaagt ctacttgata gataaaacga
    14341 aagacagcta acataatata aatctctgtc aaaacaatcg taaagcttgc tgccaaatta
    14401 gcgattgaaa ctaacgattg ctttctagga atcaaggaca agcgaatacc atttgacgat
    14461 tgatttgctt gctgatcctg tacattgcgg accccccaca tcgtcccgta gagaatcgcc
    14521 attccaatca gagtgaagaa ataaaaactt ttgaaactca tattacgacc tgcatgaact
    14581 tcttgaaaag atacttcttg ttcaaaaaca gtcatgatgg gcgcgagatc aacgttctcc
    14641 acttctgcca ctttactcga aattatacca atacgctgta aaaattgatc catgacgttt
    14701 ttcatcaacg tctgactcat tcctgcttga cccacatgga gcgaaacaga tgaaccatca
    14761 atgagataaa aaccagccaa atcatctgca tttagctttt cattcgcttc tacttccgat
    14821 agttcctgta cttcaaataa tggtttatca ttgacttcga cttgttctaa gactgtggta
    14881 aaattttccg aaagcgctgt ttgatccgct ttgattacaa ttccagtatt aatcgtttca
    14941 agattgccac cttgatcaat ctcgccaaag gccgccataa acatcaatcc taaaagaatt
    15001 ggaaaggcca atgtccaaaa caacatggat ttatttcgca gcagtatttt tgtgcgatat
    15061 ttataaaaat gccaaaacat tatttcccct cctctagtct ctcaaatcct tgccagtaat
    15121 ctctaaaaag acatcattca aggacgcttc tttggttgct atttttccgt aactgatctt
    15181 ttcttcttgt aagattgtaa gaatcggcaa caatactgta tttagttcag tagttgttac
    15241 ttggagctga ccttttttat aagcgattcc atcagaagct attgttcgct gaatcgctga
    15301 caacacattt tctgggagta atgggatgtc gatcgtcaaa cgatccgccg tccgtaccat
    15361 atcttttaga gattctttgg ttcctttagc aatcacctgt ccttgatcca tgatcacgat
    15421 ctggtcgcaa agaatttcta cttcttccat gtaatgtgtt gtatagacaa tggttgctcc
    15481 ttgtcgattc agctccttga tactttctaa aattttattc cgactttggg ggtctacagc
    15541 tactgtcggc tcatctaaaa aaattaaccg cggcttgtgg gcaatcccac atgcgatgtt
    15601 caaccgacgt ttcaagccac cactaagttc tcctggacga aacgaaacat atttttctaa
    15661 tcccactaat tgaatcacct cttcaatcaa ggctttcctt ttctcttttt cggaaacata
    15721 taaaccgcaa aaataatcga tattttcttg cacacttagt tcgtcaaaga ccgcaacgtc
    15781 ttgaggaacg accccaatca ttgccttgac agcataattt tcaggagtca ttgttgtccc
    15841 gaacaattta atggttcctt tgtcataatt taacaaagaa agcatacagt tgatggcagt
    15901 cgatttccca ctgccgtttg gacctaacag acctaagact tctccttctt gtacttgcag
    15961 atcaaaatga ttcaatgcta caaaatctcc atagcgtttg actaatttat caatttcgat
    16021 aatcatcatg aaaaactcct tccaaatttc ttgttgatct gactataata aagtgcaaga
    16081 aaagaaacta gtgacgattg gctaaaccac acatgacatt tgtcacaatg aaggagtctg
    16141 ctatgcataa atctcggcta ctcaacgaaa ctagctttct actggttatc tcattgcttt
    16201 tgctggtaag tcaaggattc gttgctatgc aagttatcta tttattgatc gccgttttat
    16261 tcactggtgt cagttattta ctgcctacaa agccaaattg gattttccaa gttattctat
    16321 ttcttttatg tgccttttta cctgatcttg tcctgtttct gccagcgacg atgcgagtga
    16381 tcgcccctga agaaagccac cttgattact tgtttttgtt aggttttttg ttgatttcga
    16441 cggttggtaa tcatcccccc atcaaggtcg cctttgtcgt aattttgctg tttttttcct
    16501 tttacttacg aaaaaaagat caacgaacga ccgatcttga gaaaaagtac attgtctcca
    16561 aagatactca ttgggaaaaa caacaagagt tatcggaaca aaataaagca ttggccctgt
    16621 cacaagaaac acaattagcc ttgcaaattg tggaagaacg gaaccgaatt gcccgagaca
    16681 tccatgacaa tgtcggtcat ctgctgtcaa gtgcgatttt acaaattggt gcgcttgaaa
    16741 tgatcaatca agaaccaaaa ctaaaagaac cattagaaaa tttaagtgcc accgttcaca
    16801 ctgggatgga tcggattcgt caaagtgtcc atgacctcca tgagacatcg atctcttttc
    16861 aacactcctt agagtttctg atcgctgatt ttccttcttc tgttacgatt gaaggaaact
    16921 tgtttgaagg attgaatgaa acgcagcaaa actgcttctt aatggtcatc aaagaagcct
    16981 taaccaacag tatcaaacat agtcatgcca aaaccgttgt tttacatttt cgaacgttgc
    17041 cggcctttta tcgcttgcaa atcacgaatg atggcaccag cccacaaaaa aattctgctc
    17101 ccggtattgg tttgaaaacg atgcgtcaac gtgttcaaga acaaaaggga caagtccata
    17161 ttttccaaga aaaccagcaa tttcagttaa cgatcatttt accaagggag ggacaccaat
    17221 gataacagta gtcattattg atgatgatcc atttgtgacg aaatcgttac aaaccatttt
    17281 agaaagcacg aaagagatcc gtgtgttagg cattggtcat tgtgccaagg atgcgctagt
    17341 cttatacgaa acccatcgtc ccgatgtttt gctgacggac atccgcatgc ctaaacaaac
    17401 gggcatcgat gctgccaaag aaattttggc taaatttccc aaagccatcc ttttgctttt
    17461 gacgacattc aaagatgaag aatacatccg cgaagcattt tccatcggtg tcaaagggta
    17521 tttaatcaaa cagaacttgc aagcgattat tccttctgtc aaatccgctt ataatggaca
    17581 ggccgtattt ggtaatgaaa tcatcgagac tttcactcaa atgatgaaga ataaaccgac
    17641 gattgatagt catgcttatg cttcttttag tgaacgtgag cttgcaatca tcaaagaagt
    17701 agctgccggg aaaaacaata aagaaattgc agatgccttg tacttgagtg acggcactgt
    17761 tcgcaattac atcagccaat tactagaaaa gctagattta cgggatcgca cgcaattggc
    17821 tatctatttt tatcagcatt tgcaataagt cttatctaac acaaaaaaac ctgaatgatg
    17881 ctccttcgca tcattcaggt ttttttcaca tcttctattc aattgtcccg caaaccattt
    17941 cctcacgatt gaaagaatca aggattcgca gggctgccag gattcgttgg attgttcggc
    18001 gtgttaggcg gaaaaatagg cgccttgtct aacgtttttt ctagttttga ttcttcacga
    18061 tcgtctcgtt ctacatcagg actttcattc aatttgaatt ctttttccat ctcatctaac
    18121 tttttaaagt cattgccaga ttcttttgct aatttcgcta atttatcttc ggtttcttta
    18181 gccataaagt aacgcccccc ttttgatttc agcttagcat atcacactga aaaaacaaaa
    18241 gaaaacgcta accaacgatc atttttagct atttctcatc tttaaaatca agcatttttt
    18301 gccggtataa aatcgtaggg agaactgttt tccataccaa tcatcggatc tactaactgg
    18361 ttatcgatca cttctgctgt aagaacgaaa tctttagaaa cagcaggttc tacctcttct
    18421 ttcttttctt ctccaacaaa tgaagaaaac agatctggtt ccactgtttc cattacttgg
    18481 tttttcgttt cattcgtccc actgtcatct tctacaggta gcttttcttt tacaacccgt
    18541 ggctcttttg tatagttccc atcttcaaat ggatcatttc cgaccaaaga atcttcataa
    18601 gctgctagtt tggtccggag agtgatattc attttatcat tatccatgat ccttgatttc
    18661 aacgtagtca tccggcttgc tgattcattc gctacacaag tgacataggc ttgctcttcc
    18721 cctaacaaaa tcaacagttc cttactgcga gtcactgccg tgtagagcaa attacgctga
    18781 agcatccgct gatattgatg gaccatgggc aaaataacca tcttgaattc actgccttgt
    18841 gatttatgga ttgagcaaca ataagaaaga gtgattttat tccattcgtt tcttttataa
    18901 gtgacctcat ttgcatcaaa ctggataact aattcatcca ctttatcttc agaatctttt
    18961 gccaagatga tacccgtgat ttctcccata tctccattga atacattcaa ttcgggagta
    19021 ttgactaatt ggaggacttt gtctcctatc cgataaacgg tgtcgttcca atgtacttcc
    19081 ttttttccag gtttccctgg attgaatatc tcctgcatca ttttatttaa cgcatcgatc
    19141 cctgcaggtc cccgatacat tggtgctaat acttggatat cttgtgcacg gaatccctta
    19201 tttttcgctt tttccacaat tttagagatc aagggttcaa tttgataagc atcacttcgg
    19261 aaataggaac gatctttttg attctttgtg aaatcttttg gcagacgtcc ttctttgatc
    19321 tcatgggcaa gaggaataat actagaacca tcaccttgcc gataaatctg attgagttcc
    19381 attttgggaa tttgttcgat ttccaaaaga tcatggagta cttgaccagg accgacagag
    19441 ggaagctgat ctttgtctcc tactaagata acttgcatat tcgtcggaat cgctttgaat
    19501 aatgtgttag ccagccatgt atcgaccata gacatctcat caacaatcaa aagtccgcct
    19561 tctaattctt tcgtactgac tgaaggtgtt ttttcccgtc cattcagccc cagaagacga
    19621 tggatcgtac tggcaggcag acctgtcgtt tcgttcattc gtttagctgc tcggccagtt
    19681 ggtgctgcaa gtagaatagg aaaaatttct tgggtatatt ttgaaggatc caattccagt
    19741 ccgttcaact ctgcaaaaag attgacgatt ccgttgatca cagtagtttt ccccgtacct
    19801 ggtcctcctg ttaaaataaa tagaggggaa cggattgctt cagcgatagc ttgttcttgc
    19861 gaaggtccat actgtatgcc gaatattttt tcgatacgcc gaatattctt ctgcacttct
    19921 ttttcaggat aatggatttc ttttttacga ctgagaaggc gctggatcga attaccgatc
    19981 ccccattcag aaaagtacaa actgttttcg taaagattag tctcttcttg ttgtatcttt
    20041 ccttcttcaa caagttggat aattacagta gcaacctgat caggctggat ttcaattggg
    20101 cgacttgttt ctaaaatacg gatcgtttgc tgaagcaatg tctccgccgg tacgtatgta
    20161 tctcctgttt gaactgcatg ctgaaaaatc tgatgcaaga tagctgctcg aattcttctc
    20221 ggtgaatctg ctgcaatacc gatttgttcg gctagattat ctgcacgttt gaacccgatt
    20281 ccttctatgt cttcgactag ctggtacgga ttttcttcga tgatatccaa agactcgttc
    20341 ttataggcct ggtagatact gaaagctaac tgactaccga acccgtaacg attcagtccc
    20401 atgatcactt gatccatccc gtggtttagc cgaatcgtct cagcaagcat ttctcttttt
    20461 ggcttcgtca aaccggagat ttgttttaat accgaaggat cagcaatgat tttttcgata
    20521 gcatcttctc ccagcaattc tacaattttt tctgctgttt tcttcccgat tcctgggaat
    20581 ttttcgctgg aaagatacgc tacgatccca tttgcagttg ttggcttttc ttgctcatat
    20641 ctttcgagtt taagctgcct tccgtattta ggatgatcca ctaattctcc ataaaaacga
    20701 taagtctcat tttcttgaat atcgccgaaa cttccggtga ctacaatctc tttttcacgg
    20761 taatcagctg tggtttcact gatgtgtacc aaaagcactt tgtaaaaatt actaggattt
    20821 tgaaaaaaaa tcgcggcaac tgtaccaacg aaataaggca tttcttctgg ctgcatcgga
    20881 agccgcctcc ttcttctata gtaaaggttc tggtgctgtt tcagttccta aaaaatctgt
    20941 ttggttccat actttcagtt catatggcaa taatctgcaa ggctcttcag tctctaaaat
    21001 cgtcaaacta ctattgaaca atccacccat ttcacgcaac tcacttagct cttttcctgt
    21061 catccattga atggctgcag ttaaagaagc tccatgtcct acgaataaaa gtgggccatc
    21121 tttatgtcgg gcaaccgcat ctgctacaac agtttcgata cgtgtgattg ccgattcaat
    21181 cgtttctccg tgaaaaggag aaggatcata cagatctaaa tgattacgca aattcggcag
    21241 attatccgga tatttctggt acatttcatc gatcgactga ccttctaaag tacccagccc
    21301 tagttctctt agcttatctg tataaaaaat ctctaccgga cgagcaagct ctttgttgat
    21361 accttctgct gtatcacgtg ctcgctttgc tgtactggaa taaattgctt taaaaggaac
    21421 atattttacc tttttcccaa atgctttgat ttcttcgtaa ctttgtggca aaagcggaga
    21481 atcgccattg ctcccttgaa aacgtctttc ctgattccat tgtgtcttcc catgtcgggt
    21541 aaaatatagt tccatacgca gcaaactcct gtctttcttt tattttactc tataataaac
    21601 tgtctaatcc agcttgtcat tttcatagtg atgcgtcaat tctaaattag ggaatccttg
    21661 ctggcgaagt gcttcataca ctatcattgc agctgtattc gataaattca atgagcggac
    21721 atgctcatca ttcataggga tgcgtaaaca cttttcttcg ttttcacgca taaatgtttc
    21781 tggcaaaccg gttgtttctt ttccaaataa aaagaagtgc tcttctccat cagcaaaatt
    21841 ttcgtcacta tatactttat gggcaaattt tgagatcaaa tgtagtttac gattacctaa
    21901 aaatgttaaa aaagcttcta aattactgtg gtaagtaata tttacatcat tccaataatc
    21961 caaccctgcc cgttttagat gtttgtcatc agttgaaaaa cctagtggct cgattaaatg
    22021 aagcggagta ttcgtggctg cacacgtacg ggcaatattt ccagtgtttg cagggattaa
    22081 tggttcaaat aaaacgatat gattcatcag gatcattcct ctcatattta tgttcgtttc
    22141 tattttctga caatttgatt tttgcataaa aaaaaacgta tcaactcggt gtcagtcact
    22201 gcgagtcgtt acgttagtga agctcttctc actaactttt atcagaacag gaacctgata
    22261 aaaaccaatg aaaaaacaac ttgttgctaa tataacaccc tcaacttttt tttgcaacct
    22321 ctactcatta aaaactgcta gaaaaacgtt ttctcttagt tccttttaat aattctatgt
    22381 caattgtaat cttcttcaaa ggattcttat ataagtctgc tttgattcgc tcttctacct
    22441 gccattctaa aggtgacatg gccaaaagat ctttttgatt ttcctctggt atctcaaatt
    22501 cataggttac acgctgacgt tcagttaatt tcattgcttc agcaaacttg gctactacac
    22561 gttcattgga ataactctgt ttttcaggtt gattcggata aaaagcctga cgtagttcct
    22621 ttaaataatt ttcttgtggc accaccttca acacttctcc atcttctgag agtacacgtt
    22681 ggaattcttg gtaatgagaa ggcgaaaaga tatttaaaac cgcgttgaat gtttgatcag
    22741 caaacggcaa attggtcaaa tcagctacac accaaaatgc ttcggcagat tgttctgtag
    22801 ctgcgtagac tccttctttt gaaatatcaa atccaacagc attttgaatc tttttcaatt
    22861 ttgaaagttc attcaagaag ctaccttctc cgcagccaac atctaatagc gagacttctt
    22921 caggtaaatg ctgcgcaata gtctcaataa cgggctgata catgccagat tggatcattc
    22981 tacgccttgg aacgaacatt ccttgatcgt attctgtttt aaccgcatgg gataaaaaat
    23041 aaagtgttcc ttttttggaa aggtcaaaac ggtgattatt tttgcatatc aagccattat
    23101 cagacttttt tacttcttct tggcaaaccg gacagcggaa cagctgatga ttttctgcca
    23161 aaaactggcg tcctcggtca atttttttta acatagttcc ccctcatttc tgttttatct
    23221 taccgttaga tgctcacttt ggcaactgct aaaaatatga tacaatgcct agaagaaagt
    23281 gaaagataaa ggagaagatt agatgatcaa agaattttgc gctgaaaact acacatctat
    23341 tcctctagca attgctagag gagccgatcg gatcgaattg tgtgacaact tagcggtcgg
    23401 tggcacaacc ccgagtactg gtgtgatcga agaaacgtta agttatgctg gggaaaaaga
    23461 tatccccgta atgacgatta ttcgtcctag agggggcgac ttcgtctata acgatatcga
    23521 attgaaaatc atgcatactg atttgattga agcaaagaaa ttagggacgg atggcgtagt
    23581 atttggttgt cttactcctt caggctggct agacgaagag gccttagaac tattgatcga
    23641 aacagcagaa gggttgcaga tcaccttcca tatggctttt gacagtattc ctgaagatcg
    23701 gcaattcgaa gcaatcgatt ggctggtaga acatggtgtt gaccgtatct tgactcatgg
    23761 cggtcctgct gacaaaacta ttgtagaaaa tttcccacgg cttaaagaat tgattgccta
    23821 tacagatggc cgcttaatca ttttgcctgg tggtggtgtc actgcagaga atgcagcaca
    23881 tgtagctaaa gaattgaacg taagcgaagt acacggaacc aaaatcgtgg atcttcagtt
    23941 ataatcttat aaacaaaaaa acatttgcaa caggcataat gcttattgca aatgtttttg
    24001 tttttttaca taaaatagcc cataaataaa gaagcaatat tgaaataaat cagtacactc
    24061 gtcaaatcgc tcaaagtcgt gataaaggga ccgcttgcta ctgctggatc aaatcctaga
    24121 cgatccatta gcatcgggat taaactacct gcaagattcg ccactgtgat tgcaaatagc
    24181 atagccatcc ctatgacaaa ccccaagata aaattatgtt tccaaaaacc tacgattaga
    24241 aaaatcgtga tccccgtaac agctcctgtg acaacgccgg tcaagacttc agccaaaaca
    24301 agtcgaacaa atccatgatc tttctcatca gaaatcgcaa gtcgacgaac agccacagct
    24361 agcgactggg ttccagcatt accggcagtt cctgtaatca gcgaaatgaa gactgctaaa
    24421 atactcgctt cacttaccaa gtcttcatag tgactgatta aagaagcggt cgacatacct
    24481 aaaaacaaca gagtgatcag ccaaggcaaa cgtcgagacg ctgcctttac tggattttca
    24541 ttaatttctt ccacattgac cccggcaagt cctgaataat cgctagcagc ttcatcatca
    24601 ataacgtcga tgatatcatc gactgtgacg attcctaaca aatgatcatc ataatcggta
    24661 acaggaagag ctaagaagtc ataatctcta aatgtttgtg ccacatcttc ttggtcgtcg
    24721 cctacatgaa ctgaaagcac tcgttcgctc atcagatcag aaatcattgc gtcgtcatca
    24781 ttcacgatta ggtcgcgcag agaaataacc cctactagct gaccttcttg attaacgaca
    24841 tagatgtaat agattgtttc agctacatct gcttcgttct tcagcacgta catcgcagag
    24901 cgaacagttt gattggctac gatcgaaaca aattcagttg tcatgatcgc gccagccgtt
    24961 tcatcctcgt aatggagaag ttccttgatt tcactggcat cgtctgccga catcaaactt
    25021 aaatatttag caatttgctt tttatccagc gtattaagca agtcaacagc attatctgta
    25081 tacatctcag aaagcatatc agctgcatag ctaggacgca tttcatctag gtattctttc
    25141 atatgttcgt tgtcttcttc aatcacatcg aacatatccg ctaattcttt aggcgacaga
    25201 taggaataga tcagctgcct gttctcttct gtaagagatt ggtagaattg cccctgctca
    25261 tagatatgca tctctaaaaa actgtttctg aatgcctgaa tattattttc ccttaaatct
    25321 tgagtcaact gggcaaatct ttcctctaat tcttgatttt catccaaacc aatctcacct
    25381 cgccttattc ttcaaaccgt ctcacaactg cctccatgtc tgcggctaaa ggctgcttga
    25441 actccagcaa ctcttctgta aatggatggt aaaaacgcag atcataacaa tgaagggctt
    25501 gtctttttat tcccagatcc attcgcccgc catacatatc atcaccgagc agggcacagc
    25561 caattgcgga aaaatgaact cttatttgat gtgtccttcc tgtatgcagc tggatatcaa
    25621 ctaacgcgat ttctgttagt cgcttctcta tccagtactc agtctgtgcc gaacgtcctt
    25681 catcagagac cattcgcttc aatagcgaag aataatctcg ggcgatcggc aaatcgatat
    25741 gtccatgtgc cgataaatct gaaagccttc cactgactaa ggcctgatat tttttcacaa
    25801 acttcttttc acgaagttgc atatctaatt ttgcgtgcgc aaatccatgc tttgcaaaaa
    25861 gcatcaagcc agatgtatcc cgatctagtc gcgtcaccac atggattact tggttttcgt
    25921 aaccttgttt tttataataa gcctttacac gattagccat tgtcccattt ggatggtatt
    25981 gggcaggaat agacgaaacg cctgctggtt tatttactac cagaacgtgt tcatcttcat
    26041 acacgatatc gatcggtgtt tcatccaaaa gtacggtttc atgttctcct tcagcaggaa
    26101 tcacaatggt taccttatca tccttagcta gagaaaacaa gacattttct acttgatcat
    26161 taactttgat ttgaccgcct tgaaatttta ttttcgctaa cagacccttt gagatccctt
    26221 tttcttttaa aaaatatttt acttgctgag aatgatcttt ctcataatcc catgtaaact
    26281 gcatatcaat cctcactaat aaaggcatct ttcacacgat gccaaaaatg catatgtcga
    26341 taagaagcaa aatggatccg ttcttctgct attcgataat aaattgagcg gatatttgct
    26401 tttgcgacat tcaattgatc aaccgttaca agataatctt ctgtattttc caattttatt
    26461 tctagccatt catcatgggc aatcacgatc ggagaaccca aagtacgaaa cactcgatta
    26521 ttcagtgaag caatctctgc caactgaaag gcattgatac taggatggat cacagcaccg
    26581 ccgacacttt tattatatgc tgtcgaacca gtaggcgtag aaatcgtcaa tccatctcct
    26641 ctaaaacttt caaacagctc gtttttgatg tatacatcgg ctaccatcgt acgattggcc
    26701 cgtttaatcg ttgattcatt cagtgctaaa aaatgctggt ccggtgtttc atccaaataa
    26761 ctgatccgaa catccagcaa gggataacta acactttgtt cccggttcgt tcgtaagctg
    26821 tctactagtt cttccagctc gtaatcccgc caatccgtgt aaaaccctaa atgacctgta
    26881 tgcacgccca agaaacgcac atcattcaga caatgactgt agtgatggaa cgccgacaat
    26941 aatgtgccgt ctccgccgac tgagatgacc aattctggct catgatcatc tcgttgaatc
    27001 cctgcttgtt ctaaaagtgc gttcaaacgt tctgtcactt ctatagtctt tgcttcttta
    27061 ttatgaacga tcgctacttt catcccctgc ctcctctctt tcgctttctt tcttcaaaag
    27121 cgttcgatag tagacatcat ctgattttcc tcttccatgg gagaataacc gctgtgcttc
    27181 ttggatttct tcacggatac tggacatctc ttcatccaac tgataggcag cttctgccgc
    27241 acgctgaagt cgttcactca tttcttctgg aaaaacgcct tgatatttat aattcagcga
    27301 atgctcaatc gttgcccaga aattcatcgc aagagtccga atctggattt ccgccaaaat
    27361 tttcttctca ccgctgatca gctgtacagg atactcgatc accagatgat aagaacggta
    27421 gccacttgct tttttatttg tgatgtaatc tcgttcttgg agaactttca tgtcttttct
    27481 tttacggatg atctcaacaa cttgatggat atcttctaca aactgacaca tgatccttaa
    27541 cccggcaata tcagacattt cttcttctaa acgtcccatt ggaatatgcc gtaattgtga
    27601 ttttgttaca atactatcga ctggcttgac tctcccagtg acaaattcaa tcgggacatg
    27661 tcgattttgt tcacggaact gtttgcggat cccccgcagt ttcactttca attctgatac
    27721 agtttgttca tatggtgcta aaaatgcttc ccaatctcgt tccatagctg atacctctac
    27781 ttcattaaca ttcattgttt attctaccat atatgcaaag ttttttcgta ctcttgattt
    27841 atgcattgac caaaaaaaga aaattaggca aaatagacaa aaaggagtcg atcaaatgac
    27901 acaaatgctt gaaatcgaat ataaatctat gctgacaaag caagagtacg agatattcat
    27961 cgctcactat caattaacag ataaagattt ccgtgtacag actaatattt atttcgatac
    28021 cgttgatgaa cagctgaaga aacaaaattg tggcctacgg atccgtttcg ttggtcatca
    28081 agctgaatat acattaaaaa cacctgctga aaaagggcgt ctcgaaacaa cggatacctt
    28141 tataacagaa gaagcagaag catttatctt cgaaaaacgt ctgcctcgtt caggcgcagt
    28201 ttttcaaaaa ctttctgatt tgaatatcga tcctgcttca ttaatccaaa ccggaaagct
    28261 tacaacaaaa cgagctgaat tccctattga agaaggactt ttagctattg atgaaagctg
    28321 gggagacaat ctacacgatt ttgaactgga actagaggta ggggatgcat ctgtgggaaa
    28381 aattgctttt ataaattttt taaaacgttt ttctttacct tatcgtcctg ctaagaacaa
    28441 gatccaacgt atgcttgaag ctaaaaatta gaaacctttc attttcacaa ggcacttgag
    28501 ttcatttttt tacttttttc tgttaagtta taggatgtga aagaatctag tcactagttg
    28561 tatagaatct attttggggg agattcatat gatcgaaatc tatttatttg ttaatccttt
    28621 aggggaatac tgtttcgaaa tggaacaaca gttattacaa tttatcgaag aagagtacgg
    28681 gacaccatca aaagaaaaga tgcaatttcg ctttcttcct ttggtcaatt tgcagacaat
    28741 tggggatgtc atgcaacaaa aaggaatttc tcagaacaat cttgaggaac gtaatcactt
    28801 attctcaatg acttattctg cagcattgga ctgcaaagcg gcgcagtttc aaggaaagaa
    28861 aaaaggccgc cgttttttga tcaaattgca agaagcagtc ggctgtaatg acataccata
    28921 ttctcaagaa cttgctgaat ccatatttga agaaatcggc ggcgatttga atatgtttaa
    28981 agaagatcgt cagtctgact tcgttaaaga agtattcttt tctgatcaaa aaattgctcg
    29041 ggaaatgggc atcacaaaac acccttctgc agtcgtttac aattatgcct gcgaccgtga
    29101 ttttggtgtt ttagtagaag taaaaggtac aagtagcctt tggagaatca cagagctgtg
    29161 taaaacaaca gataactcat atcaagtttt taatggtgaa catttacata caaatgattc
    29221 tcagttccat tcaagaacta gccatttaca cttgctttct aattaaaa
//
LOCUS       DAMYXM010000026        27549 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.027, whole genome shotgun sequence.
ACCESSION   DAMYXM010000026 DAMYXM010000000
VERSION     DAMYXM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.027"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            246..1724
                     /locus_tag="PNS21_001405"
     CDS             246..1724
                     /locus_tag="PNS21_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5159119.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            2001..4061
                     /locus_tag="PNS21_001406"
     CDS             2001..4061
                     /locus_tag="PNS21_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HDI5159120.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4144..4713
                     /locus_tag="PNS21_001407"
     CDS             4144..4713
                     /locus_tag="PNS21_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5159121.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4888..5694)
                     /locus_tag="PNS21_001408"
     CDS             complement(4888..5694)
                     /locus_tag="PNS21_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159122.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5832..7346
                     /locus_tag="PNS21_001409"
     CDS             5832..7346
                     /locus_tag="PNS21_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HDI5159123.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(7749..8732)
                     /locus_tag="PNS21_001410"
     CDS             complement(7749..8732)
                     /locus_tag="PNS21_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HDI5159124.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(8737..9384)
                     /locus_tag="PNS21_001411"
     CDS             complement(8737..9384)
                     /locus_tag="PNS21_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI5159125.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(9469..10101)
                     /locus_tag="PNS21_001412"
     CDS             complement(9469..10101)
                     /locus_tag="PNS21_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDI5159126.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(10117..10668)
                     /gene="coaC"
                     /locus_tag="PNS21_001413"
     CDS             complement(10117..10668)
                     /gene="coaC"
                     /locus_tag="PNS21_001413"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HDI5159127.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(10665..11402)
                     /locus_tag="PNS21_001414"
     CDS             complement(10665..11402)
                     /locus_tag="PNS21_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HDI5159128.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(11399..12388)
                     /locus_tag="PNS21_001415"
     CDS             complement(11399..12388)
                     /locus_tag="PNS21_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDI5159129.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            12646..13188
                     /locus_tag="PNS21_001416"
     CDS             12646..13188
                     /locus_tag="PNS21_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI5159130.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            13205..14290
                     /locus_tag="PNS21_001417"
     CDS             13205..14290
                     /locus_tag="PNS21_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5159131.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            14302..15108
                     /locus_tag="PNS21_001418"
     CDS             14302..15108
                     /locus_tag="PNS21_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5159132.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            15109..15936
                     /locus_tag="PNS21_001419"
     CDS             15109..15936
                     /locus_tag="PNS21_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5159133.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            15933..17006
                     /locus_tag="PNS21_001420"
     CDS             15933..17006
                     /locus_tag="PNS21_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI5159134.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            17434..20130
                     /locus_tag="PNS21_001421"
     CDS             17434..20130
                     /locus_tag="PNS21_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDI5159135.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            20207..20695
                     /locus_tag="PNS21_001422"
     CDS             20207..20695
                     /locus_tag="PNS21_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI5159136.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            20707..21201
                     /locus_tag="PNS21_001423"
     CDS             20707..21201
                     /locus_tag="PNS21_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDI5159137.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            21222..22031
                     /locus_tag="PNS21_001424"
     CDS             21222..22031
                     /locus_tag="PNS21_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDI5159138.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            22028..22849
                     /locus_tag="PNS21_001425"
     CDS             22028..22849
                     /locus_tag="PNS21_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDI5159139.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            22900..24999
                     /locus_tag="PNS21_001426"
     CDS             22900..24999
                     /locus_tag="PNS21_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HDI5159140.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            24996..26114
                     /locus_tag="PNS21_001427"
     CDS             24996..26114
                     /locus_tag="PNS21_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDI5159141.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            26153..27031
                     /locus_tag="PNS21_001428"
     CDS             26153..27031
                     /locus_tag="PNS21_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDI5159142.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            27222..27374
                     /locus_tag="PNS21_001429"
     CDS             27222..27374
                     /locus_tag="PNS21_001429"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDI5159143.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         8812 a         4722 c         5745 g         8270 t
ORIGIN      
        1 cttttttgtg gttgtttacg caaatggcgc aaatcttgta cattttaaca caataaaaca
       61 aaaaatatag aatatttcct cataaatttt tccatttttt gcaatattct caaaaaaaag
      121 ataaaaaatg agaataactg atgaaaaaac aagcataatc catcataaaa tcagtatagg
      181 tgaaaagatg aactttcatc tgcatatcaa gtaattggta tgaggcattt tttgaggtgt
      241 aggatatgaa gttggattac cttatagaag aggaagatag agaaaaactg ctgctagcta
      301 gatatttaga atttgagaaa aagaattatg tagtgctgcc taagttatat gaaacattag
      361 gtttgtctaa atttaaaaca agaaattata tcatagaatt aaataaagaa tttactgaat
      421 tcaaaacaaa gccagagatc caaatttcag aagaagcaga gatccatgtc gaacgtctga
      481 atttgacgga tatcaaaaat ttaagaagaa tctactttca acgatcaaaa attgctcgac
      541 tgttaacgga gatcgtggat tcaaaagtaa cgatcgaaaa atttgcagag aatcattttt
      601 taagcatatc aagagcctat gtaaaaagaa gagaattagt ctcttttctt gaacagcatc
      661 aaataaagtt aaagaaaaac atattagttg gggaagaaat aaacatacgg aacgtattat
      721 ttggggtata tttcggggtc tttaatggtt tttctatgcc atttagagaa agcgtcatga
      781 atgaagcaaa gagtatcgtg caatattttg aatatctgtt caatttaaaa ctgtctgcaa
      841 caaaacgaat caaattcaat ttgctaacca ctattagttt gttgagggcc tatgggaatc
      901 aaccaatcgg aaaacgtttc ttttcagatg aatactttaa gagctcagaa ggaatgaaga
      961 tcatcaagga tttatcacat atttcttttc ttcatatccc taaagagcag atcattgatg
     1021 agatttctta tatcatgttg tttttgatca cggaagaaga atttgaatta gatggcgcat
     1081 ttggacttgc ggaagctcat tttcatgata tccacatgat tagtcaaaaa atgtcagtgg
     1141 aattgattga tcaattacca ttttcgattg aaatctctga agaagaaaga ctatatatac
     1201 gaaaagaact tcgtatgggt atgatgcatg tcaatcgaaa aaattggata ttcgactttg
     1261 agatgactag ctttacgtct aaaaagcagc tacagttttt cttcgagacg tatccaatat
     1321 tcagtacagc aatccaaaag gtcatacaag agaataaacg ctatttcttg ggaaaagatg
     1381 aacgtttcat gattcgctta ttttatgatt atctcttttt attggtcggt atttgtccgg
     1441 tatcatattt tgaagcaccg attcatgtct gtattgattt ttcgcaagga tctgcttata
     1501 ccgattacat tatttctcaa gtgaaaggat ttaaaaatta caatattgtg attgagagtc
     1561 gaatcacttc tcgtacgaat atttatttat ctgattgttt acttgaaaaa tttaataaag
     1621 aacagataat ttggaaaaat ccaccgactc cagatgactg ggaatatttt ggaaatacca
     1681 tcatcagaga aaaaaataga catttaactg aaaaaatcag ctagtattta tggatggcag
     1741 ctttgtctca catatttatt agctgattta tgtttttttc aaagaaaagg taactagata
     1801 taactagata taactagata tattacttaa caaaattaaa aagtacataa gttattattc
     1861 cgattttctt gaaacaataa aaaatcaaaa aaaatactct cctaatagtc catcttcttt
     1921 tgattttatt tatataatga aatcatctta tgaaaattac tacttcatgt ttagacaaga
     1981 gtagggagga ctaagaataa atgtgttcaa aaagacgttc aaaacaacta aataaacacg
     2041 cttgtcgttt agataaggaa cggtcgtgtt ctgaaaaaca ctacgttaag tttatgatgc
     2101 gcacgatgct gatcagttta tctgtaggtg tctatatgca tgtacccgtt caggcaacga
     2161 cacaaaccat caaggtcgta cgtacctctg tttctgagac cactgattcc cctatcacta
     2221 ttactcatac tttcactgat gcaaatggaa aacagctact tcctgcattg acttcctata
     2281 ataaagtcag ttatcctcca acgataaacc actactggtt aattcctaac aaaacattat
     2341 ggacatcgga aaccggaagc agtgtgccgc tttctatctt ttccgatatg actgatccta
     2401 caaaaggtga tcagttaaaa cagatcactg atattttgaa tcatgctttg acagatataa
     2461 aggacggtgg aagcgcacaa gtttcttatg tatatgaaaa agattattca cagtttgaaa
     2521 gaaagaaaat cgatgttctg gcagggacaa aagtttcgat atctgatctg gtagaaaggc
     2581 tgaagaacgc agatggaaca caaggagata tgtccaaagt aatttcaaac aatgggaata
     2641 tcgtggatac gactaataca ggaacatcta ctatttcctt gtcctatttt gatccaattg
     2701 ctttaaaaac aatgactgca tctgctgtga tccatgttcg tgctgatcaa acagctatca
     2761 aaggaaaaga tcttcactta agaacaggag agagttacgc ccctaaagat cttgttgaat
     2821 atgtaacaaa aagcgatgga acttcaggaa ataaagaaga aatcaaaatg aacgggacag
     2881 tagatactac aactccggga gaatatcccg ttgtattaac ttatatagac tcgaaaacat
     2941 caaatgtggc agaaacaaca gctgtagttt atgtagaagc atctgctgct tcatcgactg
     3001 tcatcactca ttttatagat agtcaaacaa ataaagaact tcaagcagaa acacatatga
     3061 gtagtgtaaa tcgtattcct gatttgcgcc tacctgctga attttctgat cgagtgccaa
     3121 atatagattt atcgacttat gaacaaaatg ggaaagtcac ttctatttct gattggatgc
     3181 aagcaaacgg attagacaga aaaagtcact ggtcggagat ccttgaagta cttcactcag
     3241 aactgatgaa taataggaag caatcaggag gggaaattac ctttacttat gtttatatac
     3301 cagacaatcg ttcattagaa ggtcatgatc ttattgttca tccaaacgaa aaaataaaca
     3361 aagaagagtt gatccaatca gcaacaaatt ccttaggtaa acccgtaccg ttaaaggatg
     3421 tatcggttac aatcaatgaa gtaccattgg aggatggtta tttgaccaaa caaacaggtg
     3481 attttacgat tatgtatagt tatcatgacc cgtattcact ccaagaactc catgctcagt
     3541 cactattaca tgttttacca ttaaacaaac agatagatca aaaagaacaa aaaagacaag
     3601 aagaaagctt gtcagaaaaa aaacaacaaa tggctcatgc agtttctcca acagggaaag
     3661 taaaacaaac aagaagccta gagttagcaa gaatatctag aaaaatctca gaatctaata
     3721 aaacgataag gcaagaagct catttggccc cacaagtttc tggattggaa aatgaaattt
     3781 tagagcctaa aataaaacaa caatcagaaa aagaatttgt tgctaaacag tcggaagatc
     3841 agaaggaatc agatgaccag aaggaaacga agaaaaaacg cttgtcaact attgattcca
     3901 ccgatgagca tctgcagccg caagtaccac ttcctagtcc gggaacaccc gcacctggtg
     3961 gaggtattcc ttcttctgct ttaggagatt tcatgcgagg tatggcagga acatgggtat
     4021 acgctaatcg aaatgaattt gacgcagatg actggttctg atttatatct ctatctgtta
     4081 tagtcaaata agtagctgag tcatatgtta tttggcataa tggagacgag gagggataga
     4141 aaaatgttag ataaaacgtt accttacgca gaaatctgga tgacaagacc acttcaacaa
     4201 aatgttccaa tcacgccttt agcggaaggt tttgaatttc gattttatca aaaagaagat
     4261 gaatctgctt gggcggagat tgagacttcc gttggggaat ttcaaacgac agatgaagca
     4321 cttgagtatt ttcaacaaaa ctttgctcct tatcctgaag aacttgaaag aaggatgttt
     4381 tttgtagaga caaaaaaagg agaaaaagta gcaacctgta ccgcttggcg aaaaaaatca
     4441 agagagcaag tgtatccggt ttttcattgg ttagcagtga aaccagatta ccaaggaaaa
     4501 gggctcgcaa aagcattgac tgccagagtc ttgcaagaat ttcctaccct ccatacggac
     4561 ggaccaatct atcttcacac acagacatgg agccatcagg cgattgctct ttataaaaaa
     4621 atgggtttta cttttattgc tgaaaatcta gacggcacac ctaatccaga ttatgaaaaa
     4681 gttatgcagg ttttaagtaa attagataaa taacgaaatg gtgaaataag tcctcagtat
     4741 tgggataaaa aacgaaatag tcattcctca tatcttactt ttttgagtta atactatcgg
     4801 ataactaatg gcatgccata cattcaaaaa agaaggggca gtgacaaaag tattgtcact
     4861 gccccttctc ttgtttttta gcatgtttta tcgtttgatt tttttaggaa taaaattctt
     4921 gatgaacgta aatttttggt aatcgtttcg actatagagg taaccaaata cactgaaaac
     4981 aactgctcca ataaaattaa caaataaatc cttcatagta tccatgattc ccacgtctaa
     5041 gtaaccattt tggatgattg ttttatctgt tttaccatct gcattttgac tttcaatgat
     5101 tgtccgagtg atatctggaa catgatagac agtgtttccg tctttactta actccacact
     5161 attgatttgc tggatgatcg tatctttttg catatcgaat tgcagtaatt tatctgctga
     5221 aaattccaca aactcccaca taacaccaac tgtcatcgaa aaacagaagg aaacaactgc
     5281 tagtaataaa ggagtcaagt tcatggtttt tgtattttta ttgagcaaat aaacaaggga
     5341 aaagccgaca cccccagcta aaaatccgct gaatgcatgt agaaaagtat cccacattga
     5401 aaaatagcca taaaaatcac ttaactctcc taaaactgtt gaactaaaaa taaaagataa
     5461 caaaatgatt tctagcatat ttggaatctg tatctttaat atcttttcca gaaaataagg
     5521 gatttcaaat aaaattaatg tcagtaataa aagcattgta tttccccagt gttggttcca
     5581 gacctgatcc actagcataa taacgacgat gatacgtaat aaaatataga ttccgtttgt
     5641 gattctacct gcagtttttg tactgttcgt aaaaaaggtt ttcaaatgat tcataaaata
     5701 actccttcat gtatatcctt aattatacta agaagcgtgt gtttaccaaa gctatatgac
     5761 ttaaagaaaa acagagttga tattgtgaaa aaaatcactt aatatagtat aaggagaaaa
     5821 gggggataca aatgaaaaaa ttagttgctg gtttattaac agccgttgca cttcttgcta
     5881 tgagcgcatg cggcgcagat cagaaaaata cagcgcagac aagttctagt acgaaggaat
     5941 ctgccgaagt atcatcaggc gcttctgaac aagtatatac agatccaaaa gaaatgaaag
     6001 aatcttatga tgtcgtgatt attggagctg gtggtgcagg gatggcagca gctatcgaag
     6061 caaaagatgc tgggttgaat ccagtaattt tggaaaagat gcctgttgcc ggaggaaaca
     6121 catctaaatc atcaagcggt atgaatgctt cagaaacaaa atttcaaaaa gaagaaggaa
     6181 tcaaagactc gaatgatgcc ttctatgaag aaacgctaaa aggtggtgga ggaacgaatg
     6241 ataaagaaat gcttcgatac tttgtcgatc actctgctga tgcggtcgat tggctagatc
     6301 aaaacggtat caaactggac aacttaacga tcactggagg gatgagtgaa aagaggacac
     6361 atcgtccgtc tgatggctcg gctattggtg gatacttagt ggaggggtta ttgaataacg
     6421 tccatgagcg ggaaatccct atttttgtca acgcagatgt gacagacatt aagaaaaatg
     6481 gggaaacggt caatgaagtg actgtacatg ttcaaggaga agaaccaaaa gaggtcaccg
     6541 gaaaagcaat cgtagttacc acaggcggtt ttggtgccag caaagaattt attgaaaaat
     6601 atcgtcccga tttagctgat tatattacga caaatcaaga aggatcaact ggagatggta
     6661 tcaccatgat tgaaaaatta ggtggacaaa ccgttgatat ggataaaata caaattcatc
     6721 caactgtgca gcaggatgag ggggtcttga ttggtgaggt tgttcgcgga gaaggagcaa
     6781 tcttggtcga tcaagagggg aaaagatttg tcaatgaaat ggatacaagg gataaagtat
     6841 ctgcagcaat tacagcattg cctgaaaaat cagcttattt gatttttgac caaggcgtgc
     6901 gtgatcgtgc cacagctatt gaattttatg accaaaaagg atatgtcaca actggaaaga
     6961 caatggaaga attagcagaa aaaatcgatt taccaaaaga aacactagca aaaacagttg
     7021 atggatggaa tacggatgtt gcggagaaaa aagatgctca attcaaccgt gccacagcca
     7081 tggaacatga cttatcgtct ccgaattatt atgcgatcaa aatcgcacca ggaatccatt
     7141 acacgatggg cggtgtgaaa atcaatacga acactgaagt attagataaa gaaaaccagc
     7201 caattagggg gttatatgct gctggtgaag tcacaggtgg cttacatgga aacaaccgga
     7261 tcggtggaaa ttcagttgcg gagatcatta tttttgggcg acaagcgggc atacaagcag
     7321 ctcgatttgc ttcggaaaat gaatgaaata gaacgtaaat aaaaagaggt tgtgaaatca
     7381 gcgttttgag ctaataccga gggtcagctg attgaacact gtttagtgaa gaggttgtga
     7441 aaaagctgcc ctgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
     7501 aacttggttg gacttattac cgcagatgca agcttttgaa caccgtttat tcggaaaaag
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aagcagaaga aattccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttgatca
     7741 gcatgatttt attcagttat cttcaaaatg atttttccga tgtttttgtt tgcttccata
     7801 tattcatgcg cttgtttgac tgcgctgaat tggaatgtac ggtcaatgat tggccgaatc
     7861 ttgccatttt ctagaaaagg cccagctttt tgcataaaag tttgtgcgag ctctgcttta
     7921 tactgatcac ttctaggtgt caataatgta ccaagcagtt ggacacgttt cccaatcaat
     7981 acaccaaggt ctatctcagg cacgatggtc ccacctagca taccaatcaa gacccaacgt
     8041 ccatcaacgg ctatagaagc taaattcttt tcccagtaag aagcgccgat gaaatccaaa
     8101 ataacatcga ctccccgacc ttcagtgatt tttcgtacct cttcttgaaa gtcttgtttt
     8161 ttatagttga tcaattcatc tgcaccaagt tctttacaaa aggcgagttt ttcttctgat
     8221 cctgcagtaa cgatgatttt cgcgtgagaa agctgtctag ctaattggat cgctgctgtt
     8281 cccacaccac ttgctcctgc atggatcaag acagtttcgc cttcttgtaa ttttccaagc
     8341 cagtaaagtg tttgataagc agtcaaaaaa acttctgaga ttccagcagc agaaacatag
     8401 tctagcgtat cagggagaag gattgcacga tcggcaggca tcacagcata ttcggcataa
     8461 ccacctagat tgactaatcc gtatactttt gtcccaggag taaaagtgga gatatcactt
     8521 ttattctcaa ctacctctcc agctacttcg actcccaaaa tagggtaggg aggtttcaaa
     8581 gagactgatt ctcttttcat aatatctgtt cgattgacag ctgctgtatg gactttgatc
     8641 agtaattcgc cttgttttgg tttaggaaca ggcacttcaa tttgttctaa tacatctaca
     8701 gaaccaggat gcttgattga aatggcacgc atggatctat tcctcctttg cccattcttg
     8761 gaatgctgta ttcaactctt tgctaccttc agaaagtact agagtcgtac gagaaatatc
     8821 cgtttcttcc actagagcgg caagtacagc agcaactgta gagcgagtga tcgtttttga
     8881 tgccttctca ttaggcttag ttaattgaat ggaagtaact tcttcatcgt tagtcaatgt
     8941 gactggttga agaatgacaa aatctaagtt agttcgtttc agccattcat ctgcataatg
     9001 tttagtaata taataatctg tcatggactc aggccatttt gctggttcat ctgcataaac
     9061 agcactgacc atcagatagc gagagacatg cgcattttca gctgcgatca ctgttttgat
     9121 tgcgccatcc aagtctactt gcagaagatt ctttccttga gagccggcag caaaaatcaa
     9181 tacatcgatg cctttgaatg cttcggtcat tttttccacg gaccaagtta aatcaaaaga
     9241 gacatacgaa acattctctt tttttactaa tgtttgggaa gcaacatctc tcacccctgc
     9301 aaaaatttca tgggaacttg gagctaaatc ttttatcagg tgtcggccaa tttgtccgtt
     9361 tgcacctaca acaaaaattt tcatttggat catcctttcg acgtcaaatc tttccattca
     9421 atagtaacac caaaagagaa aaaaatcaga gatcggctac gatgactatc agttagtaag
     9481 agaagggtgc ttgaagtatc ttaacaagac attgcatact gctgcagtcg caagacctgc
     9541 gacgattcct tcaggtatac cacttgtaaa aataaccgct agaatagctg aataaacagc
     9601 atttaaattt gttccgatga ctttggcata atcttgctgg aataaaaagt agatcatatt
     9661 catcactaaa atcgtattgg tcaatgaacc ggaaagtcca gctaagaata aagaaacgga
     9721 atctgctttt tgtttcatta gtttgcgcag acctttataa acgaaataag gaaccacccc
     9781 aatcaagatg cgaggaataa acgcgatcag caaggctttc cagcttccat gttctgttcc
     9841 gatgactggg ataaacggag aaaagacaaa agacaaaggt gtttggataa ctgtgctacg
     9901 gatcatgctg atcagtccaa atgtaccgcc aagaaaagca cctagtcttg gtcctaaaac
     9961 gatcgaagca atgattacag gaatatgcat ggttgttgca ttgattggac cgatagggat
    10021 aaaacctaaa ggcgtaacag ctaataaaat caaaatagct aaaaacatcg cagttaatgt
    10081 aaagtttttt gtatttttca taagtactcc ttttttctaa gcatttcttt cattaagagt
    10141 cagcatgacc tgttcgatga tcgtctgaat atcagctaag gcaccttttc caaaatctcc
    10201 acaagctaaa agagattctc ttggttcaat ttcctgatag ccgacagatc tcaatgtttc
    10261 cagattacgc tgagtgatcg ggttttgata catattcgtg ttcatggcag gggcaatcaa
    10321 ttttggtgtt cccactggaa gcgccattgc gactgttgaa agaagatcgt ctgcaatgcc
    10381 gtttgctagc ttgccgataa tgtttgctgt agcaggcgcg atgagaaaca agtcagcttg
    10441 ttttgctagt tcgatatgat tgattacctg cggatttgat tcttgcatga cgtctgtatg
    10501 gactgcgcgc ttcgacagag attgcaaggt taatggcgtg ataaaatgtg tgctgctttt
    10561 agacataatc acatctacct gggcacctaa tttgacgaat tgacttgtaa gatcagcgct
    10621 tttgtaagca gaaatacttc cagtaacgcc tagtaagatc tttttcattt tgattcctcc
    10681 agtctaattt tctcagtaat caatgcagca atctcagatt tactttgagc ttcttccact
    10741 gttccatctt tagataagag atagccgtgg tgtgtctcgc catggatttc cgtcaaatca
    10801 tttgcgaaaa taaaatcagt tttgttattt atcagactat tttttgcgac tgccagaaga
    10861 gattcctttg gtacatctac taatagtttg aaaccaatga tttttgcttt tggctgccat
    10921 tcacgtagat aagaaatgat ttttggtgtt ttcttcaaaa cgagcacaag atgctcggtg
    10981 ttagaagaga tctttgaaac agtggtatct gtctgttcat tttccgcaaa ccaattgtcc
    11041 agttcttctt gcgttgaaga agtgggtagg ttttttgcca gctgttcttc tgaaaaacta
    11101 aaagcaggag taaaatcact gactgccata ctgtggataa tcgcatcgta gtgttctttt
    11161 ttcgttagtt cttctaattg taaaaataag tccagtgcag attcgatcgt ataaatcgtt
    11221 atattggagt gtcttttcgg tttcagtgct tgtttcgtag tcacgtaatc gattgtggta
    11281 tccggattgg cagctaaata atcagctaat gcttgcccta atcgtcctgt cgaatgattt
    11341 gtaatagcac gtacttgatc gattttttca gtcgttcctc cagctgtgat tagaatcttc
    11401 atttatggtc acctacattt actagtgttc ttccttggtg tgttccagca agtagtttag
    11461 atgctgtttc aggcaattga tccagagtaa tctcttggac gaccagttca tctgcaatct
    11521 gcaaatccgc catctgttgc cataaagaaa gacgttgatc tattggtaca ttgacagaat
    11581 caattccaat caactggatg tttcgcagaa taaaaggcaa gacagtcgcg tttagtttga
    11641 ttcccccagc attgccacat aaagctactg caccgttata agagatcaat ggaagaagac
    11701 tgcttagctg ctcgccacct actgtgtcga tgacatagtc gatttgctgt tttcctaaag
    11761 cttttgttgt ctctggcaga aattcatcag gtcgtacgac ttgcaaagca cctagacttt
    11821 ttagccatgt gacagactct tcttttctgg aaaaggcaat gattgactgg aatcctagtt
    11881 tatgtaacag ggctatcgct gtactgccta caccgccaga agcaccagta acgacaactt
    11941 ttttatcgga ggtcatacct tgacggatta aagcgttcac acacaacata gcagtaaacc
    12001 ctgctgtacc aaggatcatc gcttgacgca gcgtcatatt ttttggtaga ggaactaacc
    12061 agtctcctgg tactttttga tattggctat aaccgcctgg atgggtgacg ccaagcccaa
    12121 aaccagtagc aagtacaaga tcttcttttg aaaaacgtgg attgttactt tggacgacag
    12181 ttccggataa atcaatccct ggagtcatcg gatatgttcg tatcacctga ccagactcgc
    12241 tcattgccaa tgcatcttta taattgacat cagaataggc aatttttacc aagacttcat
    12301 tttcagccaa ctcagtaaat ggcagatcac tgattgctgt atggaaaaca ggttccttta
    12361 ttacctgaaa agatcgaaaa gcgttcattc gtgagacact ccttttaaaa ttttctttta
    12421 tcataccata aagcctactc ttttattctt gttcttccgt aaatttagac aaaaattagg
    12481 tgaaaaataa agaatcaaac aactagaatt tattccaaaa gataaagaaa tattcagcaa
    12541 aaaaaatttt acaaagggtt gcctttttca ataaatttcg tatactaata cgagtggtag
    12601 taaacaggaa gtttactatt agaaaacttc gattcggagg aaattatgga aattggtgag
    12661 aagttaagaa atttgcgcat ccaaaagaat ttgacacaag aagaactagg cgaacgcaca
    12721 gatttgtcaa aagggtatat ttcccagttg gaacgagatt taagttcacc atcaatggaa
    12781 acattctttt ctattcttga agtcttaggt gtgactccag aagaattttt tcatcaagaa
    12841 gcagcgaatc tgcaagtggt ttattcaaaa gaagatcata cggtttattt cgatgaagaa
    12901 aacgggtatg aattggaatg gctcgtggct gattcgaatg aaaaagaaat ggagcctgtc
    12961 ttgctgactt ttgaaggtgc aggagaatat aagacgtttg aaccttcatt atctgaaacg
    13021 tttatttttg tattagaagg ggaattagag ttaagcttag gagagacggt ctatgtggca
    13081 aaagcaggac agtcgatcta ttatcaagcg acaaaacaac atcaattaag gaatcatctc
    13141 aaaaaacgaa caaaagtatt gattgttgca acgcaatcat atttgtaata tagggggtaa
    13201 aaacgtgggg aaaccaatca tttcatttga tcaagtagtg aaacgctatg atgatgaaac
    13261 agtattaaaa aaagtaagtt ttgagatcga acaaggaaaa ttttatacac tgcttggtcc
    13321 ttccggctgt ggtaaaacaa cgatcctacg tattatagct ggattcaccg aagctacaga
    13381 aggggacatc tattttgaag ggaaacggtt gaatgatttg ccagctaata agcgacaagt
    13441 aaatacagtc ttccaagatt atgcactttt tcctcatatg aatgtttttg ataacgttgc
    13501 ttttggattg aagatcaaga aaatgtccaa atcagatatc gaaaagaagg taaaagatgc
    13561 tttgcgtatg gtccagcttc ctggatacga aaaacgggag attagtgaaa tgtctggtgg
    13621 tcaaagacag cgtgtagcga ttgcacgagc aattgtaaat gaaccgaaag tattgctatt
    13681 agatgaaccg ttatcggcgc ttgatttgaa actacgtaca gatatgcagt atgaactgcg
    13741 ggatctgcaa caacgattag gaatcacgtt tatttttgtt actcatgacc aagaagaagc
    13801 gttggcaatg agtgatgaga tttttgtcat gaataaaggg aaaatcgtcc aaagtggtac
    13861 acccgtagat atttatgatg agccaatcaa tcatttcgtt gctgattttg ttggggagag
    13921 caatatcgtg gacggtgtaa tgatcgaaga taatctagtt gagtttgttg gtaagcaatt
    13981 tgaatgtgtc gatggcggga tgcgtctaaa tgaaccagtt gaagttgttt tacgcccaga
    14041 ggatttgacg atcacgacac cagataaagg aaaactagtc gtaacggtcg atactcagct
    14101 gtttcgtggt gtccattatg aaatcatctg ttatgatgaa cagcaaaatg aatggatggt
    14161 ccattcaacg aaaaaagcta aagaaggaag caaagtagga ttggcctttg agccagaaga
    14221 tatccatgtc atgcgtttca acgagtctga agaagaattc gatgcacgtc ttgacagcta
    14281 cgaagaatag gggggacaag aatgaaaaca atgcgcagga tctattccgt cccttatatt
    14341 ttgtggctcg gtttattcgt gatcgcgcct gtattaatga tcatttatca atcttttttt
    14401 gatatgaatg ggcaattcac gctgaataat tatctatctt attttacgtc ggggacatcg
    14461 ctttctatga cgatcaattc tgtatggtat gcatttttga tcacgttttt cacgctgatc
    14521 atcagttacc cgactgctta ttttttaaca aaactgaagc acaagcaatt atggctgatg
    14581 ctgattattt tgcctacatg ggtgaatctg ctgcttaaag cttacgcatt tattgggatt
    14641 ttcagtgtgc acggcagcgt gaatcatttc tttgatttta tcgggatcgg gccgaaacag
    14701 atattattca cagacttcag ttttctgttc gtggcaacgt atatcgagat tccatttatg
    14761 attatgccaa ttttcaatgc attggaagag atgaatcctt ctttgatcag tgctagtaga
    14821 gatttaggcg cgaatagctt tgagaccttc cgacgagtga tttttccgtt atcattaaat
    14881 ggtgtgaaaa gtggtgtcca agcagtattt attccttctc tttctttatt catgctgact
    14941 cgtctgattg gcggaaatcg ggtgatcact ttaggaacgg ctatcgaaca gcatttcttg
    15001 gttacacaaa actgggggat gggttctaca atcggtgtga ttttaatcat tgctatgttc
    15061 atcgtgatgc tcctgacagg cgagaagaaa aaaggaggtc gctcataaat gaaaaaattt
    15121 aaatggtcgt atctttattt agggatcgtc tttttattct tatatgcacc gatcttctat
    15181 ctgatttttt attccttcaa tgaaggagga acgatgaaca gctttacagg atttacttgg
    15241 gaaaattatg aagctgtttt tgaagataca cgtttgatca ttatcgtatt gaatacgttc
    15301 atgctggctt tcttatctgc attgatcgcc acgatcatcg ggacatttgg ggctatggga
    15361 atctattaca cgaaaaaacg acgtgcacgg acagcgttgt tgagcatgaa taacatctta
    15421 ttagtctctc ctgatgttat tatcggtgct agtttcttga ttttctttac actactagga
    15481 ttcggattag gttttcagag tgtattgctt tcccatattg ctttcagcat tccaatcgtc
    15541 gtcttgatgg tcttgccaaa attgcaggaa atgaacgatt caatggtcga tgcggctaga
    15601 gaccttggtg ctaataattt ccaagtagtc aaaaatatca ttttgcctta tctttctcca
    15661 ggaatcatag ctggatattt tatggcattt acctattctc ttgatgattt tgcggttaca
    15721 tttttcgtca caggaaatgg ctttacgacg ctctcagtag agatctattc tcgtgcaaga
    15781 caaggaatca gtttagaaat caatgcgcta agtgcgttag ttttcttgtt ctccatgata
    15841 ttggtcattg gatactactt tattagtaaa gaaaaccgtc caaaacggct gaagaaaaac
    15901 agaagagtac gcgagggggt ggcacggctg cgatgaaaaa attacaatct ctgtttttag
    15961 gtattctcat cattattgcg gtgctgctgc ttggtgttcg tcaacttgaa aaaacaagtg
    16021 gcatgagtgg agcaaaggta cttaccatct ataactgggg agattatatc gatcctagtt
    16081 tgatcacgaa atttgaaaaa gaatatggat acaaagtgaa ttatgaaaca tttgattcta
    16141 atgaagcgat gttcacaaaa atccagcaag gtgggacaaa ctacgacatc gctataccaa
    16201 gtgaatatat gatccaaaaa atgatcaaag aaaaactagt acttccatta gatcattcga
    16261 agatcaaagg attaaataat attgatccac gatttttaga cttggatttt gatccagata
    16321 atcagtattc gattccatat ttttggggaa ctttagggat cgtatataac gataaattct
    16381 ttgatgcaaa tcaaattaag cattgggatg atttgtggaa gccggaatta aaagacagtt
    16441 tgatgctgat tgacggtgca cgagaagtta tggggctttc tttgaatagt ttaggctatt
    16501 ctttgaatag taagaatatg gcacagctga cagaagcagc aaataaattg aatcatttaa
    16561 caccaaatgt caaagcaatc gtggcagatg agatcaaaat gtatatgatc aatgaagaag
    16621 cctccgtggc agtgacattt tctggcgaag ctgccgatat gatgtgggaa aatgaacatt
    16681 tacattatgt gatcccacca gaaggatcca atctttggtt tgataatatc gttatgccaa
    16741 aaaccgtgaa aaataaagaa ggtgcctatg atttcatcaa ctttatgcta gaaccggaaa
    16801 atgcggcaca aaatgcagag tatattggtt attctacacc aaatgaagaa gcaaaagcgt
    16861 tattgccaaa agacatttcg tcggacgaac aattctatcc atctgatgac acgatctccc
    16921 atcttgaagt ctacaaagat ctaggatcga aatacctagg catctataat gacctattct
    16981 tagaattcaa gatgtatagg agataatggc aaaactcagt aagaatgggg acttatcaac
    17041 gttctaaaga gctaaaaatc gtcctttttc tatctgagtt ttccaatctt ctaccaaaag
    17101 cggatgaaac gccgcttttt ttgtgttatt ttattttttc taccacgttt ttctaccaga
    17161 gaaaagtgga caaaaccatt gtaaaatagg gcccgagacg ggctagtaaa tctaagattg
    17221 aaaaagttac agaaggataa agtaattgga aactatgcaa atatacttaa cccttctgta
    17281 atctattaat aaaactaata tatgaatcca gttcaatatt tgtagctatg aaaaaacata
    17341 aaaatcatat tagataaaaa ctaaattggt agataaatta ttttgtcaga tatctgaggc
    17401 aaatattcga ctatttgtgt ttcaaagtaa aaattggaat acaaatatag aatagacata
    17461 ttgcagccga tagtttatta tgcttttaga cacattaaac tggaggaaaa aatgaaaaag
    17521 agcgctaaag atcgagtttt tgatgagctg atacgactaa cagggaatat gtcttctgtc
    17581 actgccgaga aaatttcgaa gaatctaggt atctctcgtc agaatgccag ccattatttg
    17641 acgagattag tggaagataa aaaggtcgag aaattaccgg gaaagcctgt tttgtggaag
    17701 cctttagatg aatatgcggt tgtagataat acggagcaaa taaacgaagc ctttcattct
    17761 gtagttggtc atgacggaag tctgagagag gtcattcaaa aatgtatagc cgctgtcaaa
    17821 tatccgccta acggactcag tgtattaata aatggagcca cgggtgttgg aaaaagtttt
    17881 ttagccacaa aaatttttga atacgctatc catgagcaaa ttattgaaaa agatgcacca
    17941 tttgctatct taaactgtgc cgattatgcg gacaatcccg aactgctttc ggcaacactg
    18001 tttggataca agaagggggc ctttacgggg gctgaaaagg ataccgatgg tttattggca
    18061 acggccaaca acggctatct ttttctcgat gaggtccatc gcctatcgaa agaaaatcaa
    18121 gaaaagctct ttctatttat tgatactgga aactatcgtc cagtcggtga aaatgtaaat
    18181 tggcattctg ctaaggttcg ttttattttc gccacgacgg aaaaaggtga aaattattta
    18241 ttagacacct ttgatcggcg tattcaaatt agtgttatgt tgccaacttt tgaagatcgc
    18301 cccattcgag aacgattaga gctgattcaa ttattttttc aaaatgaagc ggatgtttta
    18361 caaaaagata ttattgtttc aagagaagcc ttgtctttaa tgttatctca tccctttagt
    18421 gggaatattg gtaagctgaa gaatatcatt aaaatcagtt gtgcggatgt gtatagtcga
    18481 acacaagaag aaccattaac gattgggaaa aatgagatct tgattcagtt aaatatgctg
    18541 gaatccgaag tagaaagctt gccgattgct agtttattga taaagaaaaa tcaaaagata
    18601 aaaacggatc gaatcaaacc gtttgattat gattcgattt tggaaaaaat tactcagctg
    18661 ataaatgaag gtaatttcaa tgaaaatttt tcttcgataa agaatgaagt acagcatttg
    18721 gtgcatcaca ttgataagga ttctttttta tcaatgagta gaaaattgat gattcagatg
    18781 tttagaaagg tctgggtatc cgttttagga aaaacgtacg gattgacgac gagtggacct
    18841 gttgcagaaa ttattagcag atcctatgtc atttccccaa gctataagac agacttaacc
    18901 gggataaatc gtacattagc tgcaaatcta cctcggacga cttacttatg taatcagttc
    18961 ttggagcatt taccaccgct ctcagaaaag gacgagaact ttctaagact gcttattgtc
    19021 gtgactttat ctgattatgt agatgaatcc atcgagctga agggcctgtt gcttgctcac
    19081 ggagatagta cggctagcag cattcaagca gtagtgaatc aattatgcgg caattatgtt
    19141 tttgaagcgt tggatatgcc agtagagagt aatgtgtcag agattgtcga atcgacaagg
    19201 aatttcgtga aacggcaagt gaatacagat agtctagtgc tgatcgtaga tatgggctct
    19261 ttaaatcaaa tttacacaca gatcaagaac gatttaaaag gggagttact tcttctgaat
    19321 aatttgacga cttccattgc acttgatatt ggcttgaaga tgacgatgaa tgcgccattt
    19381 aaacagattg cggaggatgc agcagacaag tattcaatca atgttcaata ctttgaaggc
    19441 ttttctcaaa gtagcaacat tatcatttcc tgtatgtcgg gtttggggat ttcggacaaa
    19501 ttgaaagatg tttttcgtca tcatttatcg gatgaatcca tcgaagtttt cacgaaggac
    19561 tacagagaac tacgtgaatt aatcgaccaa aatgatgaaa cttattttga caagacaaaa
    19621 ttggtcatca cgacttcgga tcttccacgt tccttttcaa ttcctaatat taatgtttat
    19681 gacatcttag acggtgatgg agcagccaat ttggagcata ttttgagtcc tgaaatgaag
    19741 ccggcagaat ttaaccgctt gattcaggag ctagtaaaat tcttttcgtt ggaaggaatt
    19801 gcggaccgtt tgaactttct gaacccaacg atcgtgatga gtgaagtgga aacagtcatt
    19861 tctaaatatg aaaattacta tcatttcact ttaaatggcc gtgtaaaact aaatctctat
    19921 atgcatacgg cattgatgat ggaacgttta ttcttggctc ggacagaaaa atctgaagaa
    19981 ccgaattatg atttgaaacc agatgaagat gaattttatt atgtttctag aagtattttt
    20041 caatcaatgg agatgaaata taattttaaa gtcaatgctt atgagctttc gctgctctat
    20101 gaattattag gaccgtatat tcaaaaataa agtgtctaaa aaagtgtcta atctgttttt
    20161 tttaggttag acactttttt taatgtgaat taacgtgatt aaaccaatgt ttttaaaagt
    20221 tggcacagga attgcttata taaaagtgaa cagaaagaaa ggaattaacc atatgaagaa
    20281 aattctaata gcgactcacg gtcacttagc ttcaggcttt cttagcagta ttcaattact
    20341 gacggggaag gttcaagaga ttacggtgat taatgcttat gtggatgagt gtgattttga
    20401 acaggagttg aatgtatttg taaaccaggt gaacgagcag gatcagattt ttgtttttac
    20461 cgatttattt ggcggaagtg tcaatcagaa gattaccaaa acctttttgg agaaagagat
    20521 tgctgtaact attttgtctg gctttaatct gccaattctg ctcgaaatag tattggcaac
    20581 caaggaatta acaaaggagg atattcagga actggtaaat aaatgccaac aggaattaaa
    20641 gatgaccatt cttgatgagg tacatgtagt aaatgaagag gaagattttt tttaggagga
    20701 agaaaaatga ttaaagcagt gcgaattgat gaacgattaa ttcatggaca agtagcgatg
    20761 acttggacaa agagtctaaa tttaaccggg ttagtagtag caagcgatga ggcggctacc
    20821 aacgatattc aaaagatgac attgaagatg gctgcaccat cgaatgtcaa agtcattatt
    20881 aaaacagttg atgacgcgat tcatttgtta aaagatccgc gggcgaagga catgcgcttg
    20941 atggttttag tccctaatgt caaagatgcg gtaaaagttg cgaaagcttt cccaacggaa
    21001 atcgaattaa tgaacgtggg gaatgctggg aaaatgacac cgccatcgga agataagaaa
    21061 accttatcaa aggaagtgat gttaacgacg tcagaaattc aagacttaaa agaattagtt
    21121 gaactttatc cagataccgt ttttcagcct acaccggcga tggaaaaacg ttcagctatc
    21181 tctattttga gtgattttta gaaaaaggaa ggagaaaaat tatgtatgat gcatttgtag
    21241 tcgctttggc cgtttttatt ggagttgctg gacatgaaat gttcgggatg tccatgctga
    21301 gtcgtccgat tgttgttgct ccgttagcag gagcgttgat gggggatttg cagacggggt
    21361 tgattgtcgg ggcgtcacta gaagccatct ttatgggtgt cgtcaatatc ggaatctcta
    21421 gtacggcgga gccttctttg gctgctgggc tggctacagc gttcgcaatc aaaatgggag
    21481 gaaacgtgga tgcgattatt cccattgtct ttccactagc agtattagga ttacagttaa
    21541 tgaatatgat tttttctttt gtatgtggac cgatggctgc taaatttttc aaatttgcaa
    21601 aatctggtga tcaaaaagga atcatcaatc tgcacttctt tatgtggttt gtccactatg
    21661 cgctttatgc tcttattcca ttctttgccg ttttatttgg agcagatgtt gtcaaaagcg
    21721 tcttggataa tattccagag gtcatcatga acggattaac agtcgctgga aatctgctgc
    21781 cggcagttgg gatggcgatg ctgttacgga tgctttggag taaaaatatt gctgtgtggt
    21841 ttttctttgg ggccgttgtt atggcgtatc tcgagttacc attgattgca attgccgcag
    21901 ttggaacgat tattgcaatt gttatagccc aaagagatct acaactaaaa aatatatctg
    21961 ttcatgcagt tcaaagcgat gctgctgtaa atgcaacgct tgaccaagaa gaggaggatt
    22021 tctttcaatg aaattagctg atggaatttc taaagatgag aaaaaattgg ttcggaagat
    22081 gttttggcgc tcgatgacta tgtatgcttc ggtgaatccg gtgacgatgg gcggcggtgg
    22141 tttcgcctat tctatgatgc ccttcattaa tcatttctac aaaagtgaag aagatcgagc
    22201 tgcggcgctg gaacgtcata cacagtattt ttcgacgacc attccttgcg cgtcctttgt
    22261 catggggatt gcggcatcga tggagaagga aaattctgaa aaagctggtt ttgacgtgga
    22321 aagtatcaac gcgatcaaaa cgagtttgat gggaccattg gctggaattg gcgattcatt
    22381 gttctgggga gtttggcgtg tcgttaccgc gggactagcg atcaacttag ccaatgccgg
    22441 aaatattttg gcaccaatta tctttttagt tatgttcaat atacctaatt acttgatgcg
    22501 atactatggc ggcgttttag gctattcatt aggctctaaa tatattgaaa agctgtatgc
    22561 gaacgggtta attgaaattc tgacaaaagc tgctagtatc ttgggtctga tcatggtagg
    22621 tgcaatgacg atcagtatgg tcaaattcaa taccgttttg agcttttcaa tgaagggcga
    22681 agaactaatg aatgtccaag aaatcctcga tagtattttt aaaggaattg taccgttatt
    22741 ggtaacattg agctgtttca aattactaga taaaaaagtc ggcgtcatca aaattatttt
    22801 tggaatggtc gtcttgggaa ttttattgtc attacttgga atcgcatagt aggaagctgc
    22861 agctactagg ctgcagtttt tttagcaagg agggaaaaga tgactattaa tgaagtaatt
    22921 tattcaccca aacacccatt ttttaatgct gagttaaacg acggaaatgt gactacttca
    22981 aacgaataca gctactatcg gaatattcct gatgtaaagg gattgacctt cgatgtaagt
    23041 aatcaaaaga cattagcaaa ccttgatgtt tgcgggacga ttcaaacgtt gaccttttat
    23101 cgagaaaatt tactaactga ggaaaaacca ggtgtttggg tcaataaaca gctctctcaa
    23161 accaattcgt tatccttaaa ggttgaagta aacgggattg tctaccattt gtcagaatcg
    23221 gatcatcgta ttgaagtcga tgtgattcaa gactgtttgc ctagatatat tcatcattat
    23281 tcgacgttta aggtcattgt agtatccttt gctcccatac tggctaataa gcgtttaagt
    23341 atgcttgttc aacaggttta cattgtgaat gaaacacacg aaagccttca agtttcagtg
    23401 aaagatgtgc cttcgtatca agagaagtat tcggatcagc aaaatgtgtt gatttctcaa
    23461 aaaggaaata aggacacgat cgcacaacag gaagtggcaa gcttcgccat tgcttttgtg
    23521 gacccaaatg cttttgaaga agtaatgact tttcaggaat ctgatatcga aaaatggctg
    23581 aaggaaacat tgaactattt tgctcagcta tttgggcagc tgtcaatgcc aaacgataga
    23641 gttgtccatc tctatcgtcg tgcactttat caaagctttt ctagtttcgg tatgaatcga
    23701 cagggacagg tcgtcggcag caattggggc agctatcctg ctactaatcg aatttggaat
    23761 aaggatatgt attacgcatc attgccattc accatgtttg aaccggagtt atgtcaaaaa
    23821 acggttctat ggtttgatca atatggcgtt aagtttcctg gttcaaaatt tacaggaggt
    23881 atcaatcatt ctctgagtaa ttcgttatcc agtgcgttac tggctagtct ttattttgaa
    23941 catactgggg atacaaaatt ctttgagcgg tgtccgcagg ttctactgaa tgcttcaaga
    24001 attattgagg acatattagc tcaacgtcgg tcaggggaac caatgctttt tccatctgtt
    24061 tggttatcag acgcctttgc acttggaaag tatcataccg gcagcaatct ttgtctttgg
    24121 aaggcctgta ttggacttgc agaaattttt gaagccctag aacaactgag tcttagcaag
    24181 cgatacaaaa acattgcctg taaactaaag gaatcaattt gtaaacaaat gactatcgat
    24241 ggtccttttg gtgagcaatt tttggaggga atcggtgatg aggagaaaac aacctattca
    24301 gtgaagcact atcaaaaacc gattcttgag caagggttaa tctttttaag tgatgtgatt
    24361 gtggatggaa aaattaattt attgatgcat gatggagaag aatcagacac tacgttgatg
    24421 cctttttatc aatttttaga taataaagat catttatatc agaacaccat ggccttttct
    24481 gctagttctt tcaatccaac ctatagcgag gaaatcaaag gcatcacttg gggattagaa
    24541 tccggcgcca cttttcctgg ctttatcact gttttaatga gcgagctgca caaccctcaa
    24601 gctttcgtaa cccgattaga agaactaatt tgtttggcag atttagacgg ttcgtggtgg
    24661 tggtggccgt atcgcttaga ggctcaacaa ggagaagtcg tgcgagactt tggctgcggc
    24721 aaatgcggct gggcatcggg tctttttgtc agcctaatga tcagtcaata tttcggattg
    24781 aaattcaaaa aaggagtatt gcaaattgat cctgttgata atttaacgtt ttcttggaag
    24841 aatttgaagt tcggtcaagc cttcatttcg attgaatgta cgcaatccga gttgagtatt
    24901 tcaaatttgt cagaaaagcc attgaaaata agtgatacga aaaaaattct tcacgtagaa
    24961 aagggaaaaa cgattcacat tcaaaggaga aaaagatgag tatactaaaa aatattaccc
    25021 aatttaaaaa aatccagagt cgagcaatca ctcccgaaaa tccaacaggc gagaaaggta
    25081 aagctgcgat gttgccaagc gagctaggac caagccgcaa aggtcgtggc gcaattccgc
    25141 tgacttctgg tgaagaaact gtgattgcgg atattactgg aacgggaata attcgtcata
    25201 tgtggatgac gattcgtgat cgaacagaat ttggcagttt tgttttacgt gatttaatct
    25261 taagaattta ttgggatggg tcggatagcc ctgctgttga aagtccttta ggggatttct
    25321 tttgtaatgg gtttggagag cgttgcgagg tgaactcgtt gccgatagtg gttaatccta
    25381 cagggggctt taattcctat ttcgaaatgc ctttccaaac atccgctaaa attacgatcg
    25441 agaatttaca tccgaaggat atgtcctcat ttttttatac gataaattac gctttggtgg
    25501 acgaattgga agaaaatagc ctatattttc acgcaaattg gcatcgggaa cgcattacca
    25561 aattacagga ggattataag ttaattgatc gattaaaggg tcaaggctat tacgtgggta
    25621 cttaccttgc gcttacctgc ctagaacgtt attggtgggg cgaaggcgag tttaaattct
    25681 acattgatgg ggatagtgag tatccgaccg taacctcaac aggttcagaa gattattttg
    25741 gcggggcgtg ggcctttcat gagtacgatg agttcggacg tccacatgcg aaaaactatt
    25801 caacagcctt tttagggtat ccttaccaat caaatagaga tgccacaaga gatcgttttg
    25861 aaacgggaaa attgaatgcc gttcatgcct ttggtgacga cgcaatgcca cgacatggtc
    25921 tttatcgttg gcatcttcaa gaccccatct gttttaccga agatttatcg gtcactttac
    25981 aacaaatcgg caacgatgat gttcgcttat ttgagcggca agacgatgtt gctagtgtcg
    26041 cttactggta ttcaacagaa gcttcaggaa atactaaacg tcatttcact gatcgagaac
    26101 gcttgccgcg ttaatgaagt gtttttcaca tattcgaagg gaggaaccta tgatgatata
    26161 tggggcaatt gaagctggag ggactaaatt tgtctgtgca gtcggtactg aggaattaac
    26221 gattttggaa agagtcagtt ttcccacaac aactccagtt gaaacaatga agcaggtcat
    26281 tgattttttt aaacctttta aggaacaatt ggcaggcatc ggggtcggct gctttggtcc
    26341 aattgatatt caatgcaaat caacgagttt cggtcatatc acatcaacac caaaacttgc
    26401 ttggcaaaat ttcaattttg taggaacgct aaagcaatat ttcaatattc cgattgaatg
    26461 gacaacagat gtcaatgcgg cttgctatgg tgaatatgtt gctggttctg gcaaagggtt
    26521 agcaggtgtt gcatacttca ctgtgggaac aggtatcgga ggtggtgcct tgttcaatgg
    26581 gactttcgtg gaagggttca gtcatccgga aatggggcat atgttgatca agcgacatcc
    26641 aaaggacaat ttttcaggaa actgtccctt ccaccaagat tgtttagagg ggatggcagc
    26701 aggaccagcg attgaaaaac ggttgggtgt aaaaggccag aatttattgg ctgacgattc
    26761 gttttggcag attgaagcct tttaccttgc ccaatgtgca tacaatacaa cactgatgtt
    26821 ttcgccagat agaattattt ttggcggtgg tgtaatgaaa caagaacaca tgaagaaaaa
    26881 agttcaggat aaatttgttg agcttataaa tggatatgtt gaaataccac caattgatag
    26941 ctacattatt acgcccgaat taggtgataa tgccggaata atcggcggat tagcattggc
    27001 cagaaaagcg gtaagaaata agcaaccata attaatgaat gtttcattga tactcatgtc
    27061 aaaagtattt aaccgagtaa tcaaatgatc aaatgaagtt ggataaagcg aaaatgaatt
    27121 caaataaaaa ggctctaagt caaatcgtgt tgttttcatt aaatcactga aaggaaatgc
    27181 ttctgcgttt ccttttttct ttttgttgtg cgctgtatac cttgaggata ttcactggtg
    27241 gcgtacactt aaatagtaat atattgtgtt actttatgtc tgtggtaata gttatagttg
    27301 cttaccaatg tatgttcatt tttcgaaatg ttgacttcat aatccttggc ttaatgtttg
    27361 aatttttttg gtgatagatt ttttcagccg tataaaataa atcgtaattc tattatagag
    27421 agttgtgatt ataaaaagat agcaagaaaa aaaaagaaag tttctataca ttttaaatat
    27481 tttattccta tttctattgc taaaaaagta gaaattctat atttaccttt aactctcgct
    27541 tctcttgca
//
LOCUS       DAMYXM010000027        27387 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.028, whole genome shotgun sequence.
ACCESSION   DAMYXM010000027 DAMYXM010000000
VERSION     DAMYXM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.028"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(292..492)
                     /locus_tag="PNS21_001430"
     CDS             complement(292..492)
                     /locus_tag="PNS21_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI5159144.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(797..2029)
                     /locus_tag="PNS21_001431"
     CDS             complement(797..2029)
                     /locus_tag="PNS21_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDI5159145.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2283..2852
                     /locus_tag="PNS21_001432"
     CDS             2283..2852
                     /locus_tag="PNS21_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI5159146.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLC
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3030..3470)
                     /locus_tag="PNS21_001433"
     CDS             complement(3030..3470)
                     /locus_tag="PNS21_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDI5159147.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3628..4392
                     /gene="map"
                     /locus_tag="PNS21_001434"
     CDS             3628..4392
                     /gene="map"
                     /locus_tag="PNS21_001434"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDI5159148.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4424..5347
                     /locus_tag="PNS21_001435"
     CDS             4424..5347
                     /locus_tag="PNS21_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDI5159149.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5423..6562
                     /locus_tag="PNS21_001436"
     CDS             5423..6562
                     /locus_tag="PNS21_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDI5159150.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6555..7355
                     /locus_tag="PNS21_001437"
     CDS             6555..7355
                     /locus_tag="PNS21_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDI5159151.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7355..8182
                     /locus_tag="PNS21_001438"
     CDS             7355..8182
                     /locus_tag="PNS21_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDI5159152.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8160..8894
                     /locus_tag="PNS21_001439"
     CDS             8160..8894
                     /locus_tag="PNS21_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDI5159153.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8994..9860
                     /gene="rfbA"
                     /locus_tag="PNS21_001440"
     CDS             8994..9860
                     /gene="rfbA"
                     /locus_tag="PNS21_001440"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDI5159154.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9874..10446
                     /gene="rfbC"
                     /locus_tag="PNS21_001441"
     CDS             9874..10446
                     /gene="rfbC"
                     /locus_tag="PNS21_001441"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDI5159155.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10468..11496
                     /gene="rfbB"
                     /locus_tag="PNS21_001442"
     CDS             10468..11496
                     /gene="rfbB"
                     /locus_tag="PNS21_001442"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDI5159156.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11594..12445
                     /gene="rfbD"
                     /locus_tag="PNS21_001443"
     CDS             11594..12445
                     /gene="rfbD"
                     /locus_tag="PNS21_001443"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDI5159157.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12480..14513
                     /locus_tag="PNS21_001444"
     CDS             12480..14513
                     /locus_tag="PNS21_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159158.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14557..15837
                     /locus_tag="PNS21_001445"
     CDS             14557..15837
                     /locus_tag="PNS21_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HDI5159159.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16047..16853
                     /locus_tag="PNS21_001446"
     CDS             16047..16853
                     /locus_tag="PNS21_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5159160.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16865..18085
                     /locus_tag="PNS21_001447"
     CDS             16865..18085
                     /locus_tag="PNS21_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5159161.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18075..19661
                     /locus_tag="PNS21_001448"
     CDS             18075..19661
                     /locus_tag="PNS21_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDI5159162.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19700..21838
                     /locus_tag="PNS21_001449"
     CDS             19700..21838
                     /locus_tag="PNS21_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDI5159163.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAESTHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(22206..23189)
                     /locus_tag="PNS21_001450"
     CDS             complement(22206..23189)
                     /locus_tag="PNS21_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDI5159164.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            23567..24958
                     /locus_tag="PNS21_001451"
     CDS             23567..24958
                     /locus_tag="PNS21_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDI5159165.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24974..26014
                     /locus_tag="PNS21_001452"
     CDS             24974..26014
                     /locus_tag="PNS21_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDI5159166.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            26033..27118
                     /locus_tag="PNS21_001453"
     CDS             26033..27118
                     /locus_tag="PNS21_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDI5159167.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            27151..>27387
                     /locus_tag="PNS21_001454"
     CDS             27151..>27387
                     /locus_tag="PNS21_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDI5159168.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVK"
BASE COUNT         8794 a         4559 c         5542 g         8492 t
ORIGIN      
        1 ggtcaaattt aaaccataat tgtgtttttc cttccatcag cataaaagtt cacctcaaaa
       61 agagtatacg aacgaatgtt ctttttgtga aggcggaatt taatatactg aataatagaa
      121 aacaagtact tgtgccaaat tatgtgccaa aaaaattcga attcaacaaa atgtaaacaa
      181 atagaaaacc ttctatatta agatttcctg caataacaaa cacaacagaa gacgtgtaat
      241 agttagtcag gaacgtacaa ataacccctg tatcctttgc ggtacagggg gttattttgt
      301 ataagtagac attgtagttt ctaaactgct atgtcctaaa cgtttcgata cgctaagtat
      361 atttactcct tgataaagta aaatagatgc atgcgtatgt cttagtccat caataaacag
      421 tcgaagagaa aatagggaaa aattgattac taaagaaaaa cttactaggt cgacggtgta
      481 gttgattttc atacttcaat agattcgaat taaacgattt cgattggctt ataccagtcc
      541 tatccaacga aagatttatc gttcttaact ctagcggtat aacttgaagc tttacgtaaa
      601 gccaatggga atgaactgga gatgactttt tattattttt agtaatagct gatgggttaa
      661 tacttacaac tttccttttc aaagtaccat ataaaaaaac ggtggggttg tttagcgaaa
      721 taaaggatct aagccaatag cttagatcct ttattctatg ctttatatta ttttttgcat
      781 gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa aatcggtact gtagagccat
      841 cttcacggat tccgtcgata tccatcttgt cagatcctac catgaaatct acgtgggttt
      901 gacttctatt caatccagct tctgctaatt cttcatcgga catttctgta ccgcctttca
      961 cactgaaggc ataagcagaa cctagtgcta agtgattcga tgcgttttca tcaaataatg
     1021 tattgaaaaa gatgatgcca gattgagaaa taggtgaagg gtcaggtaca agtgcgactt
     1081 ctcctaaacg acgcgcacct tcatctgtat caagcagttt tgctaagaca tcttctcctt
     1141 gttcggcaga aaaatcaaca acttttccat ctttaaaagt aaatttcata cccgagatga
     1201 tggtgccagc ataactcagt ggctttgtac ttgaaatgta accatctact cgatgactgt
     1261 caggtgctgt gaacacttct tccgtcggca tatttgccat gaatttttct ccacgagcat
     1321 tatagcttcc agcgccttcc caaagatggt ttttaggcaa tccgataatg atatctgtac
     1381 cgggagcagt atagtgaagg gcagaaaatt gttctcgatt caattcttct gctttttttg
     1441 ctaatttttc atcatgcttc ttccatgcta agacagggtc ttcttcatag acacgagtcg
     1501 ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc ttctggcagt tctgggaaga
     1561 cttttgctgc ccattgttta ccagccgcag ccacgacagt ccaactgact ttatttgctt
     1621 gagttgcctt gcgtaaattc attagtgctt tgccattcgc tgattgataa gacgctactc
     1681 gatggctatc tacaccagca aatgcatcgg gatcggcaga aacaacgctg atcctgcttg
     1741 ctcctttttc aagccattca tccgcttgat cgattttaga ttgtggtaca ttctcaatac
     1801 gatccgttgc agcatgtaaa agaaattctc tttggatctg gtcgtcagtc cattgaacga
     1861 taacctcagc ggctcctagt ttataagctt cttgtgtgat caatcgagca aggggtgcct
     1921 gttccacgct gatctgtaaa acaactgtgt gtcctttttc tgtggctacg ccagtttctg
     1981 caattaagcg tgcatatttt tttagaagtt cattaaaatc agataacata ataaaaattc
     2041 ctcctcacca ttttttctgg taactattag catgatacca tttaattatg attcaaacaa
     2101 tgaggcttta aataaaaaaa ggaggttatt tttttaaggt taagtttcat gatatatgtt
     2161 actacttgct atttcagctt gtgaagaaaa tcaaaaacta attttgcata tcaaaaaaac
     2221 atgttacaat gaaattaatc aatggtggaa gcgttataaa aaaattaagg agattaaaaa
     2281 atatggcaag aaaaaagacg attactagag aacagatttt ggcagctgct tatgaagtag
     2341 ctgccaccga tggatttaca cgatttactg cgagaaacat cgcgaacaaa atgaattgtt
     2401 ccacgcaacc gatttatctt gagtttaaaa atatggatga cttgagacaa gctctttttg
     2461 agcagatcta tgatcattta caatatgaag tgttcactgt tgagcataca gggaatacga
     2521 ttatcgatct tgcttttaat tatatccatt ttgcaagaag agagaagaag ttgtatcgtt
     2581 cactgtgctt agaagaatat ggtggaggaa aagagatgca gaatttctct tatacttact
     2641 ttgtgaatac agtgaaacgc gatccagctt atgtgaatct ttcagaagag cagatcgaat
     2701 ctttgtataa tgggacatgg atcatagcta ctgggatcgc agctttgatg tcttcaagca
     2761 tcatccatcc aactgatgaa cagattgaaa agatgatcca agacagtata gatgctatct
     2821 tgaaattcaa agaaccaatc gaaatcgatt gaatatactc aaaagttcca caaatcagta
     2881 ggtaacaaga gatgtgtcaa aataaggaaa agttcgtttt tcttacagcg tatgcatttt
     2941 taactcccga agaactcttc catgaatgca atgaataaaa gagccataca gattgaataa
     3001 atctggatgg ctcttttatt gcaagtatat taaatttttg cgactagttc tttagcgaaa
     3061 gcttctaaat tttcgatatc ttcttcttca gcattcagat ccactttaac attttcggct
     3121 ccttttgatg cgcctgtttt tgcaaaagca gcatcgaaat catccaccga tttgcagaat
     3181 tcatcgtaaa aagtatcacc agatccgcag acaccgtaaa tttttcccaa tagatccaat
     3241 tcttgcaagt cttcataaaa gtctacgatc tcatcaggca gatcgccatc atcgtatgta
     3301 taagtagcta ctacgcaaat atctgcctcc tcgaattcat ctgcatctac ttgggtacat
     3361 tcgttgattt cgacttcgat atccagattt tcaaatgctt ctgcaacaat atcggcgatt
     3421 tcttcagtgt ttcctgtcat gctagcatat acgatttttg ctaaagccat gatgtgcctc
     3481 ctcgtttata attatttaac aattatgaat tataccaaaa catcattgaa aagaaaataa
     3541 aacctaaaga cacctgggga atgtcatttg gctaaatgta tgccgctatg ttaaaatgag
     3601 agggatttgt aaaaaggaga cgaaaatatg attactttaa aatcagcacg tgaaattgaa
     3661 atgatggcag aatctggagc tttattagca gatgtacata aaaatctgcg tgattttata
     3721 aaaccaggaa tcacaagttg ggatattgag gtatttgtca gaaactatat cgagagtcat
     3781 ggcggaattg ctgcgcaaat tggctttgaa gggtatgaat atgcgacttg tatcagtata
     3841 aatgatgaaa tttgtcatgg attccctcgt aaaaagcctt tgaaaaacgg tgatttggta
     3901 aaagtcgata tgtgtatcga tttgaaaggc ggcatttctg attcttgttg ggcctatgta
     3961 gttggagaat ctacacctga gatcgaccga ttgatggaag taacgaagaa agctttgtac
     4021 atcggtattg agcaagcaca agtagggaat cggattggtg acatcggtca tgctatccaa
     4081 acttatgtag aaggcgaaaa tctagcgatc gttcgtgatt ttattggtca tggcgttggt
     4141 cctacgattc atgaagaacc agcagttcct cattacggag aagctggaaa agggttgcgg
     4201 ttgaaagaag gcatggttat cacgatcgaa cctatggtta ataccggaac ttggaaaatg
     4261 aaaatggatc ctaacggctg gactgcgtat acaagagacg gcggactttc ttgccaatat
     4321 gaacatacat tggctattac taaagatggt ccaagaattt tgacatcaca aggagaagaa
     4381 ggcacttatt gatctatcca gtcaataggt acgggagagg tacatgaaag tttggaacaa
     4441 aataaaacaa aatgaaaatc tgatgcgctt tattgagacg actcaaaagc gcatcactga
     4501 ttcagaaatg agtacgacct ctgtcgtcgt tgcttattat ttactcttgt ctttatttcc
     4561 attgattatt gcttttggta atattttacc gtttctccac atcgatcaag agactgtact
     4621 gacctattta cgagaagtga ttcctgagac cgtttaccag tttatcggtc ctgcaatcaa
     4681 agaccttttg acacaaagtt caggaggctt gttatccgtt tctgcattag cggctttatg
     4741 gtctgcaagt caaagtatca atgctttgca aattgcgatg aataaagcgt atggcgtaga
     4801 aaatagaaaa aattttttga tcgtccgttt cttctcacta attgtcattg tcttgttcat
     4861 gattgctatt agtggggtaa ctatcgttct tgggttggga cagctgattt tggatgctat
     4921 tcagccaatc gtacaaatac ccgtgaactt tatcgatcag ttccaaactt tgaagtggcc
     4981 aatcacattg gtagctttat ttgtcatcat gttcttgatt tatttaattg tccctaatgc
     5041 tcagttgaag ttgaagacga tcattccggg cacgatcttc gctactgtcg gctggatgct
     5101 gttatcacaa gtgtttagta tctatgctag gtattttgca acgaggatca gcggttatca
     5161 gatcatagga agttttattg tactcatgct ttggcttaat tttgctgcta cgatcatcat
     5221 tttgggcgga atcatcaatg cagttgttca agaatacgtg acgggtcatc aaatcaaaaa
     5281 acgtcgagat ccaagccgaa aatggttcaa taaattgaaa actaaatttt ctcataaaaa
     5341 aaagtagaaa caagagttct tattgtaaat ttatatcagt aatttataat aattatgact
     5401 cttaattaaa gtaggtgccc atatgcttgt ttcttttagt atggttataa gattttttct
     5461 gacaatttta ttatcactga tattaacacc tgtattcaaa atcatttcag tacaaacagg
     5521 tatggtcgac aaaccaaatg aaagaagaat aaataaagtt ccaatgcctt cagcgggcgg
     5581 actgcctatc tttatttcat ttgttatatc gactttattc ttattccgta aaattatccc
     5641 tcaatcgtat attgtaccga tcttgattgc ttctttagta atcatagtca caggagtgct
     5701 cgatgataaa tatgagctga cacctaaaca aaaaagtata gggatcttga ttgcttctct
     5761 tattatttat tttgtagcag atatacgtat tgattccttc acgcttcctt ttattggata
     5821 tatccaacta ggctggctta gttttcccgt aacgattttt tggatcttcg gtatcacgaa
     5881 tgcggtcaat ctgattgacg gtttggatgg gctggcggca gggatttcat tgatcggttt
     5941 aattacgata ggaatcattg gatatttttt cctccatgct tctacagtgt atattccagt
     6001 tgttattttc tgtcttgtag caagtattat cggttttttt ccttacaatt tttatccggc
     6061 aaagatttat ttaggcgata caggagctct attcctaggg tttatgatgg ccgtcctttc
     6121 actacaaggg ctgaagaacg tgacttttgt ttcatcgatt tctttgctga tcgtcatggg
     6181 agtgccggta acagacacgt tttttgctat cattaggaga aaagcaaacc gtgtttcttt
     6241 ttctacagca gataaaaaac atcttcatca ccgactaatc agtttaggct ttactcataa
     6301 aggtgctgtc ctgacgattt atgcgatggc tttgatgttt tctttcacag ctatggtcat
     6361 gaattatact ggaagaatcg gaacgatcat attgattatt gccatgttgt ttgctgcaat
     6421 tttattattt gaattgattg gtctgatcaa tgagaaacat cagtttattt taaaaacatt
     6481 gcgattttta gggaataaag agtaccgtac gcaagtaaaa cagaaatatt tgaagaaatg
     6541 gaaccgtcat atagatgaat aacaaattaa accagccgat ccttattagt attgtgacgg
     6601 caaacagtga taaaattttt cggacactag ataattttat ttcaactgta aaagatgaaa
     6661 cagcagtaaa aatccgaatt tttgacaaca attcacttcc cgagtacaaa aaaagactaa
     6721 cagagtatag gaaatatccc tacatcgata tctattttca tgatgaaaat tccggatttg
     6781 gttatggaca taatcacaat ctgatagcta gcgattctcg ctatggcatt atttgtaacc
     6841 cagatatttt agtggatgaa gagacagtta cagcattggt cttgttattg aaagaacatc
     6901 cggaatgtgc gatgttagca cctaagattc ttaatgaaga tggaacaact cagcacctca
     6961 tccgtgagca gctggctgtt tttgattacg ttttgcgctt tgtgcctttc aaatgggtca
     7021 aaaaaaagtt tgacaaacga ctggctgtgt ttgaatgccg aggattgcct actgatgcta
     7081 atagttatgt acggatgatc tcaggtagtt ttatggtagc tgatattgat aaatttaaag
     7141 agataaaggg gtttgatgag cgtttcttca tgtactttga agacaacgac ttgtgtttga
     7201 gttttgaaaa agcaggttac aagttattgt acacaccctt ttatgcagtt atccattttt
     7261 acgggaaggg tgcgcatcga aatgcaaaac tgtttcgcgt gtttttgact tctatgatga
     7321 aatttttcaa taagtggggg tggcgttttt tctgatgaaa ccagaaatat tagtagtcgt
     7381 tgtgctttat cagcaactat tcagtcaatc tccagcttat ggaccattaa gcaaagcact
     7441 tagcgaaaag caaattcagc ttattattta cgataatagt cctcaaaggc agcaaaatga
     7501 gctgtttgaa aaagaaaata tcgtttatca tcatgatccg caaaatcctg gtttagcggt
     7561 ggcttataat tttgcgttgt ctcaagcaga tgaaggaaca cgttgtctcg tcactctgga
     7621 tcaggatacg aggctagtcg acgattattt tgaactattg cgtaaagtaa cctttacaga
     7681 tgaatgtgtt gctgcagttc cgatgatttt ttctgggagc cgacaagttt ctcccctata
     7741 ttcagatcgt tatatcaatc gtacagctca agcagtagaa gttgaaggcc caacagctga
     7801 acgaatcatg gcaatcaatt caggtgttgc ctggtctgtc aaatttctaa aaaagattgg
     7861 cgggtttaat cctgcttttt ctttagattt tttggatcat tggctgtttt ggaaagtgga
     7921 tcagttggaa aaaactgtgg agatccttcc tgtacgaatg gaacatgatc tttcggtttt
     7981 agactatgaa aaagtttcaa gtaatcgata ccgttctatt ttacaagcag aaaatcgatt
     8041 ttatcatgaa tatgatcaaa ggcatttatc cagtcaccga cgacaattat tgttaagaac
     8101 agctaaacag ttcttgaccg tcaaaaaccg gcatatctgg cggatgactt tgcgctcata
     8161 tgttaagaat tggaaggtgt gaattcgtgc gttcggtttg tatagctacc tttaatggag
     8221 gcagatattt aagagagcag ttagatagta ttttatctca gctttcagca gaagatgaag
     8281 tagtcatttc tgatgatggg tcagtagatg atacatggga tatattaact gaatacgcag
     8341 aaaaagataa gcgaatccag cttttttcgg ggcctcgtca aggattgatc gctaattttg
     8401 gttatgcgat ccagcagacg aaaggagatc taatcttttt ggcagaccaa gatgatgttt
     8461 ggttggagac aaaagtgaaa aagatcactg actattttga gcagaatccg gatcagctag
     8521 tagtagtttc tgatttaatc attgtggaca gcgagctgca agagatccat gattcgtatt
     8581 tttcgtttcg aaattccaaa gaaggatggc ttaataatgt gataagaagt cactatattg
     8641 gtgctggtat ggctttccgg tcttctttaa aacccgatat tttgccgatc ccgtcagaag
     8701 tgccgatgca tgatatgtgg attggtttgc tgggaggaaa gaagactggg ttcctaagag
     8761 aaccgttaac tctgtaccga agacatgatt taaacgcgag tgagattgaa acacattcta
     8821 aattacggca aaaaatttct tggcgtgttc atctgattac ggcgttgatc aaacgaaaat
     8881 ggtttaaacg ttgataaaag atgtttaaat ccttaatatt tccatgacaa atataacaat
     8941 tgatgagata ataacaaagt gaaatttgaa atcaaaaagg agagcagata tccatgaagg
     9001 gaatcattct tgctgggggc agcggaacac gtctgtaccc tttaacaaag gcaacatcga
     9061 aacaattgat gccgatttat gacaaaccaa tgatttatta tccaatgtct actctgatgt
     9121 tggcgggaat caaagaaatt ctgattattt caacgccaca agatacacca cgtttcaagg
     9181 aattatttgg aaacggaaat gatttaggac tgcagatcga atatgcagta caagaaagtc
     9241 cagatggatt ggcacaagcg tttatcattg gagaagaatt tattggagat gatagtgtct
     9301 gcttggtatt aggggacaat atttactatg gtggcggtct gtctaaaatg ttgcaacgag
     9361 cagcttcaaa agatacaggc gcgacagtat ttggctatca tgtcaatgat ccagaacgct
     9421 ttggtgtagt agaatttgat gagaatatgc aagctttgtc gattgaagaa aagcctgctc
     9481 agccaaaatc aaattacgct gtaactggcc tttattttta tgacaatgag gtaatttcta
     9541 tcgctaaagg tatcaaacct tcagaacgtg gagaactaga gattacggat gtcaataaag
     9601 catatttgga taaaggaaaa ctatccgtag aacttatggg acgcggattt gcttggcttg
     9661 atacaggaac acatgaatca ttgcttgaag catctacctt tatcgaaaca attgaaaaac
     9721 gtcagaactt aaaagtggct tgtttggaag aaattgctta ccgtatgggc tacatcgata
     9781 aggaacaatt gctggccctt gcacagccat tgaaaaaaaa tcaatacggt caatatttat
     9841 tgaatttggc cgctgaataa ggagtcctta cagatgaaag taacagatac aaaattaaca
     9901 gatgtcaaaa tcgtagaaat ggacgtcttt ggcgatcatc gcggtttctt cacagaaagt
     9961 tattcaaaag caaaatttgc agaacatgga ttgaaatatg actttgttca agacaaccat
    10021 tcactatcag ctgaagctgg tgtccttcgg gggcttcatt tccaaaaagg agaagctgca
    10081 cagacaaaat tgattcgggt agtcactgga gcagttttag atgtagtagt ggatatccgc
    10141 aaaggtagcc cgacttatgg tcaatgggac ggatttatct tatctgaaca taatcatcga
    10201 cagttgcttg tacctaaagg atatgcccat ggattcgtta ctttgacacc aaatgtgaac
    10261 ttcctttata agtgtgataa ttactataat gcagaagcag atgggggcat cgcatttgat
    10321 gatccagatt tagcgattga ttggccaatt gatccatcaa gagcgattat gtcggaaaaa
    10381 gatcaccgtc atccgacatt aaaagaattt gaagcagaaa atccatttgt ttacggagaa
    10441 atctaagaat taaggagaaa ttttcttatg aaaaacatca ttgttaccgg aggtgccgga
    10501 ttcatcggct ctaatttcgt tcattacgtg gtaaacaatc atccagaagt gcatgtcact
    10561 gttttggata aactgactta tgcaggaaac aaagagaact tggccggact tcctagcgat
    10621 cgcgtagaat tggtcgtagg agatgttgca gatgcagaat tagtggaccg tctagtaaaa
    10681 gaagcggacg cagttgttca ttacgcggca gaatcccata acgataattc attaaaagat
    10741 cctttcccat ttgtacaaac gaatatcatt ggtacgtata cacttttaga agcttgccgc
    10801 aagtatgatg tacgctatca tcacgtatct actgacgagg tttacggcga tcttccatta
    10861 cgtgaagacc tgccaggaca tggggaaggc gaaggagaaa aattcactgc ggaaacacca
    10921 tataatcctt caagtccata ttcttctaca aaagctggtt cagatctgtt agtaaaagca
    10981 tgggtgcgtt cttttggctt acgtgcaacc atttcaaact gttcaaacaa ctatggtcct
    11041 tatcagcata ttgaaaaatt tattcctcgc caagtaacga gtatcttaag cggtattcgt
    11101 ccaaaattat atggcgaagg aaagaatgtt cgtgactgga tccatacgaa tgatcattct
    11161 tcagcagtgt ggctgatttt gacgaaaggc agaattggcg aaacctatct gatcggtgca
    11221 gacggtgaag aagataataa gaccgtgatg gaattgatct tagaaatgat gggccaacca
    11281 aaagacgcat acgaccatgt aaacgaccgc gcagggcacg atttgcgtta tgcaatcgat
    11341 tcgacaaaat tgcgagaaga gctaggttgg cagcctgagt ttacgaactt ccgtgatggg
    11401 ttggctgaca cgatcaaatg gtacgaagaa catgaagact ggtggaaaaa agaaaaagag
    11461 gccgttgaag cagcatatgc caaaaatgga caataataca gaggaaattt cactgtatta
    11521 atggaaaaac acatgtgagg caggcgctcg cctgtctctt ttttaaaata aaaatcaagg
    11581 agcggataac catatgtttt tattaactgg aggaaatgga caattaggta cagaattgcg
    11641 ccatttatta gatgaaaaaa atctgccata tgtttcaaca gatgcaaaag acttggatat
    11701 cacagatgct gagaaaacaa tggcttatat tacagaatta aaacccgatg ttattttcca
    11761 ttgcgcagct tacacagcag tagataaagc agaagaagaa gcaaaagaat tagatgagaa
    11821 aatcaatgtc gatggcacaa gaaatgtagc acttgcagca aaagctgtga atgcacaatt
    11881 cgtctatatc agtacagact atgttttcga tggaaaaaac aaaaatgtag aatacgaagt
    11941 agacgaccaa acgaatccac taaatgaata tggtcgtacg aaattattag gcgaaaaagc
    12001 agtagaagag attttagaag actattatat catacgaact tcttgggttt tcgggatcta
    12061 tggacacaat ttcgtcttca cgatgcaacg cctagcggaa acccgcgaca aactgactgt
    12121 agtaaatgac cagtttgggc gaccaacttg gacacgtact ttggcagaat ttatggtata
    12181 tgtcatcgaa gaaaaagcac catttggtat ttatcatttg tcgaacgaaa atagctgttc
    12241 ttggtatgag ttcgcaaaag aaatattgaa agatacagat gtagaagttg cacctgttac
    12301 gtcagaagaa tttccgcaaa aagcaacacg tccgcagtat tcagtcatga gcctgaaaaa
    12361 gacagaagcg ttaggttttg tcataccaac ttggcaagaa gcacttgcgc aaatgctgga
    12421 agctgccaaa aaaatgaata agtaatttcc tttttatcaa atagaatttg gagcatgata
    12481 tggaaaaaca aacaagaagt gcacgacgaa aagaaaagcc aaaagcgaat caggcagatt
    12541 cgaatttggt gcttgacctg tttctaaaac tccgctatct tattggcgtt ttagtcatcg
    12601 tggtcatcgt tacttttaat ttaaacggaa gttcacttgg tgtttgggat aaatatgtgt
    12661 cgcaaagaga cgatgggaaa aaaactgatg tgatttttgg acagaatcgg gaagtgcggt
    12721 cagacgaatg gctggtgcag acaccttttt acctctcaca agctgaagag gacttttcgt
    12781 tggtgaacga ggattattca ttaaacggac aaaacatgat cattgcgtat aattctcctg
    12841 taaaggatat tacggttatc ggaaaaccat tcaactgggg attctttttc ctcggaagag
    12901 atcgaggact ttccttttat tgggcgatga aattagtcgt catggtcttg cttggattcg
    12961 aagtactgat gatattaacg aagaggaaaa aagctctatc gctaatcggt gcatttgcgc
    13021 tgacattttc tccagcagtc cagtggtggt tcatgcaaca tgtgggcgat ttgattttct
    13081 ttactttggg actgatgatt gctttttatc attatttcta caatcatgat aaaaaatggc
    13141 tacgaacgtt gatgatgctt ttggtggtta ttttcggtat tgggtttatt ttagtgattt
    13201 acccagccca tcaagtgatg ctggcgtact tgctgatttt ttattttgtt ggcctgctta
    13261 tttactatag caagaaagtt tcatgggatc tgttggatgt agtgttgatt ggaggggcag
    13321 ttttatttat tggcggagtc atggttcatt tctggctaac gtcaaaagac gcattgttag
    13381 cttctttgaa taccttatat ccaggaaaac gggtaagtac tggaggaaag tggacgattg
    13441 gcgaattttt ttctttcctt acaaattgga aaattccttt tgaagatatc aactattcga
    13501 ataactctga agtggccctg ttctatcact tttttccaac tgtattccta gcaagtccat
    13561 ttgtgttatt tggcaaaaaa gatagcgaac aaaagttact gggacgtatt ttgatgattt
    13621 tttgtctgtt ttctatcttt tggattaccg ttgggttgcc aaaatggcta gcagaaatca
    13681 ctctgctgtc ttatgttcct ccattaagag ccatccttac ttttagtttt gcggcttgtt
    13741 tattatcgat ctggtttatc gcttatattt ggagagaaga tgttattcct atttggtata
    13801 aattcatttt gttggcagta aatgcatggt cttatttcta tgcgattacg cattcgaaaa
    13861 tggataatta tttttcggat tttgaaacca tcgcagttgc tgtgttggga acagtgattt
    13921 tagcttttgc attatttggc tggagacgct tgttttacct cgcttttgtc gccttagtta
    13981 tcgtatctgg ctttttcgtc aatccagtcg tagaaggtac aggagcgatt tttgaaaaaa
    14041 cactggcaaa agaaatccaa aaaatcgatc aacaagatcc tggtaaagtt tggttgtctg
    14101 aagatgaatt atataacttc accccaacat tgggagtgaa atccttcaat acagtacgct
    14161 tttatccaga tatagctgca tggaagaaaa tcgatccgaa agaagaatac gagaagtatt
    14221 ataatcggta tgctcataca agagctttta tcgctaagga agatacaaag ttcattttag
    14281 atcgtccaga tatgttttcg gtcacgttga attttgaaga cgcagaaaaa ttagggatcg
    14341 actatgtgat cagtaaacga ccattaactg attacaacca attatatgaa gcgcagttca
    14401 cgcagctata tggaccggat aaagacggtt atatgatttt taaagtagaa tatccggatt
    14461 atccagatgt accacaaaca caaactcagg aaaatcagct agtcgtacag taataggcta
    14521 gctgagaata atcctgaaat attagtagaa aggagagtga agataattca agcaatcgta
    14581 caaaaatttg tcaacaaaat gagtgtcggt ttcttgcttt tgttgctgac gctagtcttt
    14641 tttgtttgga cagcaggggt aggttttcca gcaactttgt ttatatataa caatagttca
    14701 ttacttatcg cttttagtgt ggctattttg ctcattttaa atgtttataa agcaaaaggc
    14761 gtcgattttc tttatctagg tgttgcgctt gcccttttca ttttcttttc ttactttcgg
    14821 gaattaagaa atgagtcttt atttggagac ttactgtttc ctttgattat tttattttgt
    14881 ttagtaatta aatggatcga gtggacaaaa tgggatcgcg gtatttatct gattgtcttt
    14941 acggtcttcc tcggaatcac gttatatcgt gtatttacag agatccaagt accagaagga
    15001 caaagcatct gggctccagg caattcgctt tctgatattt ggatcaatac gaatacaatc
    15061 ggaagttctt tgatgactct tgctttattg atcagtggat ttgccagttc ttttgaacga
    15121 tggtatgttc gaatcatcgg tgggatagca gtcgttgcag cctttttagg tatttgggtc
    15181 tgccaatcta gaggtgcatt agtcgcgttg attgtttttg cgatattgga tcttttacct
    15241 aaatcgttga tgcgtgtcat tcgtgcgcca tttattgcct acacggttac gattctttta
    15301 gctttgccga tttcttatct ggcggctgtc tctgaaaaag tcaatttgtt taccgatcga
    15361 gaagatattt ggcataaatt ctatcagacg ctaggagaaa aatctgaaca agttttacta
    15421 ggaatgaaaa catttatctt ccagcgagga aaccaatttt taggaaacca caatagttat
    15481 aattctattt tgaatatcta tggattgata ggatttggga ttgctgcttt gctgcttatc
    15541 ctttttattg gtcgcttaac gttgaaagca gatttatcca acgggcagat gacttttatc
    15601 tgggcctttt ttgcggtgat gatgcagtca tttatggagg atacattgac ttcataccca
    15661 tgggttccga ttatttatat cctgttggct atggccgttc atcgatatga tgaaccagaa
    15721 gttgcggagg aattctatgt tatggaagag gcatatgacg atatgactta tgatgataca
    15781 tcttatgagg aacaaccaga tagcgctcat tcttccagga tccaacgcta tcattgatag
    15841 gcacaaaata cgagagaggc tgtgacaacc gtcgcagcct tttttggttt attgcaattg
    15901 tctagcatct tctgtaaaat agaaacgtta gagaaaatct tgtatcatag aaaattaaga
    15961 aagaaataag aacgcagctc ggtaccaaaa atatggtaaa atggcacttg actgtaattc
    16021 actgaaaatc gcttggagga acacaaatgt ttaaagattt gtatttattt atgaaggaca
    16081 tttatcagaa cagaaaatta ttgctacagt tttcgatcaa tgacttcaaa tcaagatatg
    16141 caggatcgtt tttaggaatc ttatgggcgt ttatcaatcc gctatttact gtattgattt
    16201 attggctggt atttggtgtt ggactgaaag cacaaatgat cgatgcaaaa tacccattta
    16261 tcgtatattt agttaccggg atgattccct ggttcttctt ctcagatgca tttgcatcaa
    16321 ctacggttgt ttttcgggaa tatacttatt tagtaaagaa agtcgtattc aatattcgta
    16381 tcttgcccac gacgaaaatc ctatccaatc tgtacaccca tttattcttc attctgattg
    16441 gcatagctgt cgcgcttata aatggtatat atccttcagc aatgtcttta cagctgattt
    16501 attatttatt ttgcatgtgt gctttcttga caggattgac ttggctgacg gcatcgatcc
    16561 aaccattttt acctgatatc atgcaattaa ttacgatcgt gctgcagttg atcatgtgga
    16621 cattaccgat attatggagc cctaatcagt ttgattcagg tcttgtaagc atattgaaat
    16681 taaatccgct gtactatgtt gttcaaggtt atcgtgagtc atttttaagt gaagcttggt
    16741 tctgggagca tggaacatat actttgtatt tctgggtttt cacgttagta atgttcgtaa
    16801 tcggctctgc agtgttccgt agattgaaac cacatttttc agatgtgctg taaggagaat
    16861 agctatgaca gaatatgcaa ttagactaaa agatatcaca aaatcttata atatgtacgc
    16921 aaaaccttcg gatcgatttc gtgaagccct caatcctttt aagaaatcct atcatgatgt
    16981 attttatgcg ttgaaaaacg tgaatgtaga aataaaaaaa ggtgagatga tcggtttcgt
    17041 gggagaaaac ggatcgggaa aatctacaat gttgaaaatc attacgggag tactgacacc
    17101 aacatccggc acgatggaaa tcgaaggaaa aatctcagct ttgctagaac taggatcagg
    17161 attcaatcct gagtattctg gctatgaaaa tatctattta aatggaatgg ttctaggatt
    17221 ttcaagagaa gaagtcgatg aaatggtcga tgatatcatt gaatttgctg acatcggaga
    17281 ccatatttat caacctgtaa aaacttattc aagcgggatg ttcgttcgtc tagcttttgc
    17341 tgtagcaatc aatgtgaatc ccgatatttt gattgtggat gaagcattgg cagtaggcga
    17401 tcttgaattt caactaaaat gtatggaaaa atttacagaa atcaagaact cgggaaagac
    17461 gattttattc gtttctcatg atatcagttc gattcgtcgt ttttgtgatc gttcctattg
    17521 gttgaaaaac ggagaagtcg tcgaatatgg cgatacgatg gaagtaacga cgaattatga
    17581 aaacttttta aagaaaaaat cagttaaaac agtggatcgg aataaaaacc aagtagaacg
    17641 gttcgccgct ccagatatcg tcgacgtgac gaaagcggaa ttgttggata aagacaaaaa
    17701 tccggtggat atgctggaac aaggagaaaa actatacgtt aaagtaactt atgaagtaaa
    17761 agatgataca atcaaaaatc cggttttagg aattgcgcta cgaacagtcg acaattttta
    17821 tgtatgcgga cttaatactc tactagatgg tatcaagatt ccttggaaaa aaggagagaa
    17881 tatcttttat ctggagtatg aaaagttagg tttgttagct ggtgaatatt attttgacgt
    17941 tgcggtcttt gaagaaaatg caacggttcc tttggtatat aagacaaagt acatgaactt
    18001 gtttgtcagc ggttcttata tcggagaagg catcgtggtc ttagaccata aatgggaaga
    18061 agggactcat agcaatgaaa tatgattttg aaatggacct agatgaacaa agcagtgtgg
    18121 gaaaaattgc cgcacagatc aaaccaggaa gtaaagtttt agagtttggt cccggaaacg
    18181 gccgattaac gaaacattta atcggagcaa aacaatgtga agtgagcatt gttgagttag
    18241 ataaggaatt gttcgatttt gtcagcgaat ttgctcaaga tggattttat ggtgatatcg
    18301 aaagttttga gtgggcaaac tattatgctg gacagacatt tgattacgtc ttgtttgcgg
    18361 atgttttaga acatttggta gatcctggaa aaacattgaa gaaagtcaga gaatttttga
    18421 atgaagaagg agagatcctg attacattcc ctaacttggc gcataattct gtcatgatcg
    18481 atttgttcaa taaccaattg ccgtgggctt cttatggtct cctagatgaa acccataatt
    18541 ctttttatac tcatgatggc ttccaaaaag tttttgaaaa agcgggatta ttcattaata
    18601 ttgaagatta tttgtactta gctgtaggtg atacagaatt aaaatcaact tatgaagaat
    18661 tgccggaagc tgtacgctat gatttcaaaa tgcgaccatt tggtgaagtc taccaatact
    18721 ttttctcatt gatgaaacat ccagtagcac aaagttccat tgcagagcca caaaattcta
    18781 attatgtaaa agtgttagaa gtaacccagc aaacgaaaca agatgaaaca atccaacaaa
    18841 taccattcaa taatttcaca ggggaaaatg agacactaag tttccccgta tcagaaacaa
    18901 cagaaagaat ggtgtttcgc ttcgcgcaac aacctagctt tatcgaattc agcgcagaag
    18961 ctgccggaga aaaactaaca tttatcgatt cgaatgctgt agtcaaaaca gtaaatgatt
    19021 gttatttgtt tgatgggaaa gaattaccag agttcgttct aacggatatt tctgggaaag
    19081 aagtgactat ccattgtcac tatcgcttta tcggggaatt gacaccaaca atgaaagaat
    19141 tgttggaaac gattcgtccc atggctgaag tcacaaagca gctttctgag aaaaatgacg
    19201 aattagaaag agaaaatcat catcttcttg aggaaaatac aagactagac catacgttaa
    19261 aaacaaccac aaatcgctat tgcacattgt tggaaagtga tgaatggaca ataaaacata
    19321 gactaggacg acgtaagaaa gaaacttcga aaaaaatcca agaaaaagaa ctttctatat
    19381 gtattgacga gaaaatctgg gatgcagaaa caaaaatatt aaaaatcatc ggatggggaa
    19441 tcgctaattc ggatcgccag cccttgtcct ataagttatc agctgaccaa tcgccatttt
    19501 tcgaagcaat tcctgtttca cgagaagaag tcaatcaagc agaacagctc gcaaaaggaa
    19561 cagaagcggg atttgagtta cgaatccttt gcgagcaaga acgttcattc ttagtggaag
    19621 cagtagctca aaatggccaa tcatggatca ttgaaatgta aataatgatt aacttataaa
    19681 gataaggagt aaacgaatcg tgcaaaaaaa agaaattgcc gtatttatcg atcaaatcac
    19741 tcgtgaacgg gccactggcg atttgcttat tgtgggctgg gcaattgatg aagtgaccaa
    19801 agaaatccct acgataaagg tagaaaaaga aaatgtgatt gctgaatcaa cacatgtcgt
    19861 aagattggat atcaatcatt tatataatct tgatgttaag acccaaagcg gtttcaaggt
    19921 acgtttatca gggaaaatga gaggaaaagc catcttagac ttccaaacag cgagacatca
    19981 aaatggcata gctgtgaaat taaacggtaa atacccttat gatgacggca ttgaatcttc
    20041 atgggaaaga aaaaaaagat tactgaaaaa agggataaat tatgcaagga ctcatggcgt
    20101 aaaaaaagcg atccgtcggg tcaaattgga attgaagcca ggatcgatcg actatgcaga
    20161 gtggatcagt cgtcatgaga aaccagattt gaaagagcaa aggaaagaag cacaaggatt
    20221 tgcgtaccgt ccattgattt ccatacttat gcctgtctat aacgttgaaa tcaaatggct
    20281 cgaaaaatgc attgattcgg tactggatca aacgtatgat cattgggaac tatgtatcag
    20341 tgatgatgct tctactgatc cagctatccg aaagtgtctg gaatcctatc aagcaaaaga
    20401 tgatagaatc aaagtcgttt tccgaaaaga aaatggacac atcagtctgg caactaattc
    20461 tgcattggaa atagcagaag gagaatttat agcactctta gataatgatg atgaattacc
    20521 gccatttgca ttatatgaag tggcaaaagt gctaaacgta catccagaac tggatgtgat
    20581 ctacagtgac gaagataaaa tcgatgcaga tggcaatcgc tttgatccgc atttcaaagc
    20641 agactggtcg cctgatacat tgatgggaaa taattatatc tctcacttag gcgtatatcg
    20701 aacaagcatt gttaaagaac ttggcggttt ccgtaaaggc tatgaaggtt ctcaagatta
    20761 tgatttggtc cttcgtgtga cagaacaaat ccctgcagat catatttacc acattgaccg
    20821 agtcttgtat cattggcgga cgatcccagg atcaactgca agtaatggcg aagccaaaag
    20881 ttatatctat gattccggag tgaaagcatt gacagatgcc ctgtctagaa gaaacatcaa
    20941 aggaagcgta cgtcctggca gaatttctgg attttatgag attgcttatg acgtcttgca
    21001 agaggatttg gtcagtgtga tcattccaac gaagaatggc tatgaagatc taaaaacttg
    21061 tgtggattcg atcatcgaaa aaacgtctta ttcaaattat gaaatcatta ttgcggataa
    21121 cggaagtaca gatccaaaaa tgcaagaatt atttgcagaa tacaagcatc agctgaaaga
    21181 ccgctttatc gtagaattga ttgacattcc attcaattat tctcgaatta acaatttagc
    21241 agcagaaaaa gcaaacggca aatacttttt attcttgaat aacgatacag aagtgattga
    21301 gccagattgg atgacggtga tggtttctta tgcacagttt gatcgaatcg gctgtgtagg
    21361 agcaaaactg ttttatccag atgatacaac gcagcatgca ggagtattat taggaatcgg
    21421 tggtgttgca gggcatgcat tgaataacta tgatcgaaca cattgtggtt atttcggccg
    21481 attggtcatt gacgtcaatt acttagcagt cactgcagct tgtatgatgg tcaaggcagc
    21541 tgacttcaat gctgttaacg ggtttgatga aacattagaa gtagcattca acgatgttga
    21601 cttgtgcttg aaagtttatg aactgggtcg ttacaatgtc tatgcacatc aagcagaatt
    21661 gtaccatttc gaatctaaat caagaggtta tgaagatact ccagaaaaac aaagacgttt
    21721 tgctggtgaa atcaaaaaaa tgcaagataa atggccagca tatattgcgc atgatccatt
    21781 ttacaatgat aatttaacaa aagacggaat cggcgacttt tcgttgagac cagattaaac
    21841 tataataaaa agctgttatg ttgtgattcg ctcgcacata acggcttttt atagtttttt
    21901 gtcaaaaaaa ggcagaaggt gccgactcaa aaaaataagt cgaggttggg acagaagtgt
    21961 ttaactccga gaaataagaa gaaattcacg aaaattgctt ttcaaatttt tgtgaatttc
    22021 ggcttattta agaagaagtt gcttctgctc ccaccgttta ttcgttttta aagaatgaga
    22081 caaaagtgtt tcttactttt gtcccacgct cttattcatg ctcagagcgc caatagatac
    22141 aaaatcatag ccaaagtttc acgaacccta gctgtcaaag aatcgatatt ttattttaat
    22201 ataggctatt ttaaaataaa tggtttaagg tattcctcta caaaagccac tctatcaatt
    22261 atcatgggtt taaaataaga agtgatggag ataaccagat tgttcttagc gtcaatataa
    22321 agaacatttc cactattacc aattgctgaa tatatttcgt tctcagaatc taatacccac
    22381 cataaatagc catatctcat attttgtaag tcttctaaag cactggttct aggtttgaac
    22441 atctcgttta tccacatggg tggtacgatt tgttttcctt tatacatccc tttatttagg
    22501 cataatacac caatttttgc cagatcactt gccgataaac acaaaccata tcctgccgtc
    22561 ccgatattta gtggatcaca aaaccagaca ttggttttag gagacttatc cattgtaaac
    22621 tgcttatgtt cttcagctgt ttcagcataa taattgacat ggtttggtat ccctatagga
    22681 tcaaatagat acttattggc aaaatcaaca gttgttagct tacttgtctg atacaacaag
    22741 ccagttaata tgtgtaaaca gacagtttga tagttgaatt cgccggttat accttttctt
    22801 cctcccaata aatctagtga tgtttttgtc caatcttcac ttgaacagac ttttgtccat
    22861 ggatcgcctt tacatttgta gggtgctttc attgttagca agtgcttgat tgtaatagcg
    22921 taaatagttt tctccccgcg tttcacttta tactctggaa aataatctaa tactttatca
    22981 tcgatacttt tgattaatcc ttgctctaaa gcaatgccaa tcagcaaaga tacaatactt
    23041 ttcgttgctg acatgatatg acaagtatcg tcttttttat aaccattcca ttcatctgta
    23101 taaacttcta ccccatcctt atatgcgaca atttgacata tatttggttg tcgaaaacca
    23161 ataaaatcat gcaactcttg tttattcata agatattacc tcactttctt ctataagcat
    23221 aatgtcatta aaaatcgatt tcaatttttt cgattcttca cgtaatacag gtattcttat
    23281 ggactagaca gcgctttggg aagatggatt ctctgaaaaa tataaaggtt cactttatga
    23341 gtggataaaa aaagcagcaa atacttcgac ttctttaatt taccaacatt agtgaaaatc
    23401 gattcatctg gagatttgta atttagagtc cttgtcttga ttagaggtgt ataaagcttt
    23461 ttattagaca tttttaaatt ccattacaat ttgcttataa actaggtttt taaaggtgac
    23521 tatggtaaga taatagagat attattttgt gattaggagt gtatatatga ataaaaatgg
    23581 agaatggagt gctgcacgac ggatattcat tatattaatg gatgttattg tatacaatgc
    23641 agcaatatat agtagtttct tactgaagtt tcgtggagag atcccattac gaaatttaga
    23701 aactttccaa cactcagcaa ttattatttc agtgattttt attgcgttga acattttgtt
    23761 gggtgcatat gttttttata atcgaatgat cagtgacatc gtgtttgtca cagtcatcgg
    23821 tcaggtattg atgacactag ggatcatggt agtgactttt gcgggccgct ggtttgcttt
    23881 tccacgtctt gttatcttac tttcatttgt tctcggtacg cttttcttaa gtatctatcg
    23941 tattctcgta tataaacttt atttacgcgt aagtcgagat aaaaaggtcg tcattattgg
    24001 gttagaaaag gatgtcgcac cagcaatcca aaattttggt tcaaagaaaa acaacaaaca
    24061 taaagtcgaa gcagtcattc ttgataacta ttatgcaaac atcaaaaata tgattaaaga
    24121 catcgatatt gtctatcttg cttctcatat cgaagaaact gaaaaaatca aaatctatca
    24181 attagttact aaaaatgaaa aaaaattgtt tttgaataca acatttgaaa acttgacatt
    24241 gatcaatcca aatattatga atttcgaaga tgaaagtatt attgaagcat ctggatttag
    24301 aatccaacct gaatatgaac tattcaaacg tttgtttgat atcatcgttt cacttgtttt
    24361 attagttatt gcttcaccat ttatgttatt cacagcaatt ttagtcaaag tgacatcacc
    24421 gggaccaatc atctataaac aagtgcggat cacaaaaaat caaaaggaat tttcaatcta
    24481 taaattccgc tcgatgaccg ccacagccga agcaaaatct ggaccagttt tagctaagag
    24541 caatgatgca cgagtaacgc ctgtaggtaa atttattcga gctgtccgct ttgatgaatt
    24601 accacaaatc ttcaatgtgc tgaaaggcga tatgtcgatc gtaggaccac gtcctgaacg
    24661 tcctttcttc gtcgatcagt tcaatgcaga aaacccttat tactacttac ggcataatgt
    24721 tcgtgctggt atcactgggt atgcacaagt ctatggaaaa tatgtctctg attacaacag
    24781 taaattacgt tttgatttac tttatatcaa aaaatattca ttgatgcttg atttaaagat
    24841 cttgctgcaa accatcaaga tcctatttga taaaatgtcc tctcaaggac ttgaagaaga
    24901 cgaaaatgtg atctcattgg cagaaattgc ttttccgaat gaaaaaatct ttcgctagtt
    24961 gggaggcgta aaaatgaaca atccgaaatt cagcataatc gtaccagtat acaatgcaga
    25021 agcaactatt ggtcgaacga ttgatattct aaaagaattg gattatgaag agtacgaagt
    25081 cgttttagta aatgatggat caacagatca aacacaggaa atcattcaag aactgattga
    25141 aaatgatgaa cgttttgaat tgattactac gaccaatcaa gggcccggat tagcaagaaa
    25201 cgaaggaata agacagtcaa aaggagaata tctcttattc ttcgatgcag atgacacgcc
    25261 agtaaaaacg atcttgtcag attatgatca tttgctagaa aagagtccaa aagcggatct
    25321 tgttgttagt tcgtttgttt ttcgtacaat ggacgaggga agaatcgttt ccgaaaaaga
    25381 aaacttagtt acagaatgtc actatctaac tcacgaagag tttataaaag acatgtatca
    25441 gttaatgaat gaacagttga tgtatgttgt atggaacaaa tgctatcgta gcgatattct
    25501 gaaaaataac caaattctat ttaaaactta taatagttgt gaggatcgaa tatttaactt
    25561 acattattat gaatactgtc atcaagtgat catgaaccca aaagtagaat atatttatga
    25621 atttgaaggt gggaagggga tcactaatca ataccgacct aacaagttcg aaacatttaa
    25681 agaattctat tcattagcaa atactgtaac agacgatatt aataaagacg gtatggcggc
    25741 gcttctttta aaagggacaa cttcagttat tttttctatt tatagtacct ctgcaagaac
    25801 ttcaaaagag aagaaagacg aagcaaagca gatattgagt gatccagtga tccgagaagc
    25861 caagcaaatt gctaagacgg attcttcagc aaaaaaaata acaaagttgc tttacaatat
    25921 gccagctcca ctatttttat ctgctgtaaa agttggaagc ttcgttgaag tgaaaatgcc
    25981 agggttaatg gctgctttga agcgagtata ttagttaatg gaggaaaata taatgaaaag
    26041 aattttgcat tttcagggac gaatgggttt aggtggagca gagtctttca tgatgaattt
    26101 atatcgtaaa attgatcgta caaactatca atttgatttt ttgatttatg aagattatgc
    26161 agatgttcaa gactatcatt cagagattga aagattagga ggccgtattt ttgttgtacc
    26221 taatcctaag aaaaatattt ttaaatattt aatagaagtg aataagttac taaaaaaaga
    26281 atctttttct atagttcaca atcaggttta ttttggcggg ggaattaatt tatggttagc
    26341 caaaaagaat ggaatcagac agagaattgc tcatagccat gcaactgaag atggaaaatc
    26401 tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc aaattgttgt tacaaaatgc
    26461 aacagactac cttgccgtta gtcaagaagc tggtgaaagt ctgtttcaaa accatccctt
    26521 tgagatagtt cataatggta ttgatttaga gctttatagt aaaaattcag aagctaaggt
    26581 taataagaga aaagaattgg atatctcaat gagtactttt gttgtgggaa acattggacg
    26641 attagagaaa caaaaaaatc aatcttattt gttagagatt tttttagaaa tactaaaaat
    26701 gaattctgat tcttacttga ttttagtagg tgaagggtca cttagaaata aactgaaagc
    26761 caaagcagta gaattggata tagagaaaag agtaaaattt ttaggagaaa gaaacgatat
    26821 tcccgagtta ttatctgtga tggatgtttt tgtaatgcct tctctttatg aaggtctgcc
    26881 aatggttgga ttagaagcac aagcagcaaa attaaaatta gtcttagcag atactatttc
    26941 caaagataca aagttaacaa ataatgtgga ttttgtatca ttagatgaaa caccacaaga
    27001 atgggcaaaa aaagtaataa aagagccgta tgctaataaa ataacacaag aactaaagag
    27061 ttatgatgtt tcgtacacta ctgaacaaat ggagagtata tatagtagag atcattaaaa
    27121 atgttcaaga tattttagga gtgattattt ttgaaaatat gttatttgat attggcatac
    27181 aatgatattg gcaacttaaa gagattggtc aatcgattga acgaaaatgc agtttttatt
    27241 attcatatag ataaaaaaac tgatatagaa ccatttattg aagcatttaa atcatatgat
    27301 aatgttgaat ttttatgtaa taaaaaccgt gttaaaattt attggggtgg tttttccatt
    27361 gtacaagcag aaatcaattt agtaaag
//
LOCUS       DAMYXM010000028        26976 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.029, whole genome shotgun sequence.
ACCESSION   DAMYXM010000028 DAMYXM010000000
VERSION     DAMYXM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.029"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            19..876
                     /locus_tag="PNS21_001455"
     CDS             19..876
                     /locus_tag="PNS21_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDI5159169.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            991..1911
                     /gene="pstC"
                     /locus_tag="PNS21_001456"
     CDS             991..1911
                     /gene="pstC"
                     /locus_tag="PNS21_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDI5159170.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1911..2795
                     /gene="pstA"
                     /locus_tag="PNS21_001457"
     CDS             1911..2795
                     /gene="pstA"
                     /locus_tag="PNS21_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDI5159171.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2810..3616
                     /gene="pstB"
                     /locus_tag="PNS21_001458"
     CDS             2810..3616
                     /gene="pstB"
                     /locus_tag="PNS21_001458"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDI5159172.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3632..4390
                     /locus_tag="PNS21_001459"
     CDS             3632..4390
                     /locus_tag="PNS21_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDI5159173.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4403..5080
                     /gene="phoU"
                     /locus_tag="PNS21_001460"
     CDS             4403..5080
                     /gene="phoU"
                     /locus_tag="PNS21_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDI5159174.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5331..6349
                     /locus_tag="PNS21_001461"
                     /pseudo
     CDS             5331..6349
                     /locus_tag="PNS21_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723340.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
     gene            6346..7113
                     /locus_tag="PNS21_001462"
     CDS             6346..7113
                     /locus_tag="PNS21_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDI5159175.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7213..8781
                     /gene="liaX"
                     /locus_tag="PNS21_001463"
     CDS             7213..8781
                     /gene="liaX"
                     /locus_tag="PNS21_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HDI5159176.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9078..9398
                     /locus_tag="PNS21_001464"
     CDS             9078..9398
                     /locus_tag="PNS21_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HDI5159177.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9398..9748
                     /locus_tag="PNS21_001465"
     CDS             9398..9748
                     /locus_tag="PNS21_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDI5159178.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9915..10853
                     /locus_tag="PNS21_001466"
     CDS             9915..10853
                     /locus_tag="PNS21_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDI5159179.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10868..11698
                     /locus_tag="PNS21_001467"
     CDS             10868..11698
                     /locus_tag="PNS21_001467"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDI5159180.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11742..12767
                     /locus_tag="PNS21_001468"
     CDS             11742..12767
                     /locus_tag="PNS21_001468"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDI5159181.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12782..13720
                     /gene="galU"
                     /locus_tag="PNS21_001469"
     CDS             12782..13720
                     /gene="galU"
                     /locus_tag="PNS21_001469"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDI5159182.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13845..14285
                     /locus_tag="PNS21_001470"
     CDS             13845..14285
                     /locus_tag="PNS21_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDI5159183.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKATAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14288..14890
                     /locus_tag="PNS21_001471"
     CDS             14288..14890
                     /locus_tag="PNS21_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDI5159184.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14949..16055)
                     /locus_tag="PNS21_001472"
     CDS             complement(14949..16055)
                     /locus_tag="PNS21_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDI5159185.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16265..17266
                     /gene="ccpA"
                     /locus_tag="PNS21_001473"
     CDS             16265..17266
                     /gene="ccpA"
                     /locus_tag="PNS21_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDI5159186.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17603..19948)
                     /locus_tag="PNS21_001474"
     CDS             complement(17603..19948)
                     /locus_tag="PNS21_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDI5159187.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSISDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLSKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20227..21129
                     /locus_tag="PNS21_001475"
     CDS             20227..21129
                     /locus_tag="PNS21_001475"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDI5159188.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTAAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            21342..23117
                     /locus_tag="PNS21_001476"
     CDS             21342..23117
                     /locus_tag="PNS21_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDI5159189.1"
                     /translation="MLNTLKPLINQLTKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHFSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(23183..24769)
                     /locus_tag="PNS21_001477"
     CDS             complement(23183..24769)
                     /locus_tag="PNS21_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDI5159190.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            25020..25244
                     /locus_tag="PNS21_001478"
     CDS             25020..25244
                     /locus_tag="PNS21_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDI5159191.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            25386..>26976
                     /locus_tag="PNS21_001479"
     CDS             25386..>26976
                     /locus_tag="PNS21_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI5159192.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIAMIPLPLLAITSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYLKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVKHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLMLDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAH"
BASE COUNT         8623 a         4810 c         5655 g         7888 t
ORIGIN      
        1 gattaaagga gatttctaat gaaaaaactt aaattattct taccaataat aggattagcc
       61 attcttttat caggctgttc aaagtggatt gatcgaggag aatccattac ggctgttggc
      121 tcttctgctt tacagccttt agtagaaaca gttgctgaaa cttaccagac agaaaatcct
      181 ggaaaattca tcaatgtaca agggggcgga agcggaactg gtttgtctca agtacagtca
      241 ggagcagtcg acatcggaaa ctctgatttg tttgcggaag aaaaatcggg gatcgatgcc
      301 tcagcattag tcgatcatcg agtagcagtt gttgggatca ctcctattgt gaacaaggat
      361 attggcgtaa aagacatcag tatggaaaac ctgaaaaaac tatttacagg cgagatcaaa
      421 aactggagtg aagtcggtgg aaaagatcag ccagtagtta tattgaaccg cgcttcagga
      481 agcggaaccc gtagcacgtt tgaaaaatgg gtattggacg gagaaacagc tgttcaggca
      541 caagaacaag attcaagcgg aatggtgcgc cagatcgttg ccgacacacc cggtgcaatc
      601 agctatgttg ctttttctta tgtttccgat gatgtagata cgttgaatgt taacggtgta
      661 gaaccgacag atgaaaatgt gacaacgaac aaatggacga tttgggcata tgagcatatg
      721 tacacaagag atcatccaaa aaaactgaca aaagaatttt tagactatat cttatctgat
      781 gaagtacaag ataacatcgt tggagaacta ggttatatcc ctgtttctca tatgaaagtc
      841 gaacgtgatt ggcaaggaaa tatcgttaaa gagtaatctt tacacaaaat ttacaaactt
      901 agctaaactt ttacatgtta ttaaaaccaa attgatgttc aatttgtaca ctgagtgagt
      961 atgttaaacc tgattaaggg ggagtcatct gtggaagatg tgaaaaaagt attattaaca
     1021 aaatcaaaac gttcaaaaat ggaacagcgt ggaaaattca tcagtttttt atgtatcgcg
     1081 ctgatcgtgt tggttgtatt atctattttt tattttgtag ctagtaaagg attggccacc
     1141 ttttttactg ataagatcaa tgtatttgat tttctgtttg gtacagattg gaatccaagc
     1201 caaatcggag caaatgggaa accaatggta ggggcattgc ctatgatcac aggttcgttt
     1261 attgtgacct ttttatctgc tatcgttgcc acgccatttg ccattggtgc agcagtattc
     1321 atgacggaga tctctcctaa aaacggaacg aagatcttac agccggtcat cgagctgctt
     1381 gtaggtattc cttctgtcgt ttatggtttt atcggtttat cagttatcgt gccttttgtc
     1441 agaagtattt ttggcgggac agggtttggt atcttagctg gtacatttgt gttgttcgtt
     1501 atgatcttgc cgactgtcac aacgatgact gttgatgcat taaaggcagt gccacggcac
     1561 tatcgggaag cttcattagc gcttggtgcc acaagatggc aaacaatcta caaagtcgtg
     1621 ctacgagcag ccgtgcccgg gattttaaca gcagtcgttt ttggtatggc tcgtgcgttt
     1681 ggtgaagcct tagctatcca aatggtcatc ggtaatgcag cattgatgcc aacaggactt
     1741 actactcctg cttctacatt gacgtccatc ttaacaatgg gaatcggaaa tacaatcatg
     1801 ggaacagttg aaaataatgt gttatggtca ttagcgctga tcttgctatt gatgtcatta
     1861 ttctttaata tcgtgatccg tatgattggg aagaaaggag agctgaaata atgaacgcaa
     1921 aacgctcaga taaaattgct acaggtgttt tatatatagt ttcggggatc atcgtattga
     1981 ttttagcagc tttactactt tatatcttag ttcggggaat accgcatatt tcatggtcct
     2041 tcttgaccga accttccaaa gcctaccaag aaggcggtgg aatcgggatc cagctattta
     2101 actcgattta tttgcttttg atcacgatga tcatcagttt tcctatttct ttaggtgcgg
     2161 gtatctatct ttcagaatat gctggaaaaa actggctaac agatgtgatt cgcacgtcga
     2221 ttgaaatctt gagctcgttg ccttctgtcg ttgtcggttt attcggtttc ttggttttcg
     2281 ttattcaatt ccagtatggt ttctcgatta tttctggtgc attggctttg actgtattca
     2341 acttgcctct tttgactaga acagttgagg agtctttaca agcggtccat tacacacaac
     2401 gagaagcagg attggcctta ggtttgtcac gctgggaaac agtgataaaa gtggtggttc
     2461 cagaagcgac accagggatt ttgactggtg tgatcctaag ttctggaaga atttttggtg
     2521 aagcagctgc attgatctat acggctggac aaagtgcacc tgcgttagat tttacgaact
     2581 ggaatccatt aagtgtttca agcccgatca gcattttccg tcaagctgaa acattagctg
     2641 tccatatttg gaagatcaat acagaaggca cgatgcctga tggtgtttct gtttcggctg
     2701 gcgcttctgc cgtgttgatc attgctgttt tactgttcaa cttcggcgct cgtgcgattg
     2761 gcaaacgatt ataccgaaaa atgacttcag cctaaggaga agaagcaaaa tgaaaaaata
     2821 taactggaat gaaacaaatg tcatcaccat gccaaaagag aaagaagtag cactatatac
     2881 ggatgatcta catgtctggt atggcgacaa tgaagcaatc aaaggagtgg atctggaatt
     2941 tgaaaaaaat aagatcactg cactgattgg tccttccggc tgcggaaaat ctacgtatct
     3001 tcgttcgttg aatcgaatga atgatgggat cgctaatacg aaaatcactg ggaaaatcat
     3061 gtatcaaggc gtagatatta atgcgcctca agtggatgtt tatgagatgc gcaaacggat
     3121 tggcatggtt tttcaacgtc ctaatccgtt cagcaagtcg atttatgaca atattacatt
     3181 tgctttgaaa cagcacggcg aaaaaaacaa aaagaaatta gatgaaatcg ttgaaacaag
     3241 cttgaaacaa gctgccttgt gggaccaagt aaaagacagc ttggacaaaa gtgccttggc
     3301 tttatctggc gggcaacagc aacgtttatg tattgctcgt gcgatcgcga tgaaacctga
     3361 tatcttgctg ctagacgagc cggcaagtgc tttggaccct atttcgacag gaacggttga
     3421 agaaacactt gtaaacttga aagaagacta tacgatcatc atcgtgacgc ataacatgca
     3481 acaagcagct cgtatcagcg actatactgc ctttttctat ctaggaaaag taatggaata
     3541 tgacaaaact aacaagatat ttaccagacc aaaaattcag gcaaccgaag attatgtgtc
     3601 cggtcatttt ggttaagacg gaggcctcaa catgacaaaa gaaattattt catctaaaga
     3661 tcttcatctg tattatggaa agaaagaagc actaaaaggg atcaatctaa gtttcaatca
     3721 aggggaaatc actgcgatga tcggtccttc tggttgcggg aaatccactt atctgcgttc
     3781 tttgaaccgg atgaatgacc tgattccgaa tgtaacgatc accggaagtg ttttatataa
     3841 agacaaagac atctatggtc caaaaacaga tgttgttgaa ctaagaaaag aaatcggcat
     3901 ggtattccag cagccaaacc catttccttt ttctatctat gaaaatgtca tctatggatt
     3961 gaaattaaaa ggggaaaaag ataaaaaagt ccttgatcaa gtagttgaag aaagcctgaa
     4021 ggcagcgtct gtttgggatg atgtgaaaga caaattgcat aaaagtgctt tatctttatc
     4081 cggtgggcag cagcagcgtg tatgtatcgc acgtgttttg gcagttaatc cggaaatcat
     4141 cttattggat gaaccaacaa gtgcgcttga tcctgtttct actgggaaaa tcgaaagtat
     4201 gctgttagaa ttaagagagc ggtatacgat gatcatcgtg acccataata tgtctcaagc
     4261 ttcacgtatt tcagataaga cggcattctt tttagatggt cacttgatcg aatacaacga
     4321 tacgaagaag atattcatga atccagaaaa acaagagaca gaagattata tttcaggtcg
     4381 atttggataa ggaggaaacg gaatgctacg tacacaattt gaagaagaat tgctgaatct
     4441 ccataatcag ttctacgaaa tggggatgat ggtgagcagc gccgtgcaca agtcagttcg
     4501 tgcatacatc aaacacgata aaatcttagc ccaagaagtt atcgaaaacg atataaatat
     4561 caataacatg gaaacacgct tggagaagaa aagttttgaa atgatcgcat tgcaacagcc
     4621 agttacaact gatttgcgta tgatcattac ggtcatgaaa gctagctcgg atttagaacg
     4681 tatggccgat catgcggtgt ctgttgctaa atccacgatt cgcgtaaaag ggcagacgag
     4741 aataccggaa atcgaaaaag agatctccga tatgtccgat tatgtgaaaa aaatggtgga
     4801 caacgtccta gtagcttatg tcaaaacaga cgaaaaagac gcacgtacga tcgccaatat
     4861 ggatcaaaga gtcaatgact atttcaatcg aatctataat gcaacaatca aaaatatgca
     4921 ggaaaatcct gagacagtca tcagtggaac agactacttg aatgttgcca gttatctaga
     4981 acggatcgga gactatgtga caaacatctg tgaatggatc gtctacttgg ctacagggaa
     5041 aatcactgag ttaaatacga accacaatga gattttctaa actgaagctg tgattatgcc
     5101 gtttgtttag gaaaagaggt tgtgaaatca gcggttgtga aaaagctgtt ctgcatcaag
     5161 taataagaac agtcaaacaa aaaatagctc ctcatatttt tcgtttggct ggacttatta
     5221 ccgcagatgc aagcttttga acaccgttta ttcgggaaag ggtttgtgac aaaacatgtc
     5281 acaaaccctt ttcatcattt catagaaagg ataaagaatg caaaaaaaaa attagcatca
     5341 tcactatatt ttttatttgt gtgcttactt catgggttat tcagcatcaa gctgctttat
     5401 atgaagtacc tgtcatcgag atcgaaaaag tacgaataaa agaggatcat caattgatta
     5461 caggaagact gatgaacacg gatcaaaaag gagaatcgat ttatttatcg gttccttatc
     5521 aaaaaaatca aatagaaaca gcaaatataa aagcgagaca aatgtatcta gtccaaatgc
     5581 atggagatga atggcagcta gtagcaaaaa aaaatgatgg ctggcttttc ttcttcacaa
     5641 gtatcttcat tggcgccatg ttgcttattg gcggaaaaca gggtctattt gctttaggaa
     5701 gtctcatcat caatatcgtt ttactttact tttgttgctc atctttacta aaacagaagg
     5761 gatttctcta ttaaatatat ctatcctgtt cgtcgttata gggatgatta ttagcatatt
     5821 ggctttaaat ggatggaata gaagcagtat aatcaaaatt tcagctgcag ttacgagtgt
     5881 ttttttagca ttttttattt gtcttttgac aatgaatcat tggaaagacc aaggactgcg
     5941 ctatgaggaa ctgaattatc tgacacgacc gtatcgttct gtttttcttt ctagcgtact
     6001 gattggaaca gctggaggaa cactcgatac cgttatcaca gtcattgcga cattagaaga
     6061 attgactaag cgacaaccag aaataacagc gaaacagtta tggctttcag gaaggcaagt
     6121 gggtcgagat gtgattggct cgatgaccaa tatccttttg ttcgtctata tcagtgggtc
     6181 agttccatcg cttcttttat atttaggaaa tcaatggtcg ttcaaagaaa cgatagaaca
     6241 gcatttatca ttggaatttt tacgcgtcat tgctggcagc ttaggaatag tattatcgat
     6301 ccctatctcg gtcctctttt ttctgactgt aaggaggctg aaacgatgaa tgtacttatt
     6361 atacttgttg ggattttagc aatcagcgtg ctacttattg gcggtacgca aggtatgtat
     6421 attcttttag gcttattcat caatttaggg atctttttcc tgttgttatt cggttatcat
     6481 cagaagtggc cgatactggc attgtcggtc ataggatttt tattgattgc tgtggttatc
     6541 ttatttttta tcaacggtta taatctaaaa atgcgtgcag cttttgtgag tatactgatc
     6601 tttttatttt gtttcctact attgcttcct atcactgatt ttttagcgat ccaaggtttt
     6661 acatcggtag aactagaaga attatctggc ttagataaga ctttagcgat tgattttaga
     6721 ttacttacga gatcgttgct tctgattagt cttagtggtg ctgttttaga tgctagtgta
     6781 gcaatttcta gtgggacatt cgaagtatat caggcaaatc cgcaattatc tttttaccaa
     6841 ttgcgccatt ctagtttcgc tattgctaaa aaaatattgg cgtcaacagt aatgacactt
     6901 ctttttgcat tcatgggaag ttctttagcg ttgatcctct ggtttattga tttagacatt
     6961 ccttttcagc aagtgatcaa tgaaaaatca tttgttctag aatatacgat ggctattctt
     7021 accacattgt ctgcactact tgttcttcct ttaacctgcg tgactacagc atatctcttt
     7081 acaaaaaaga aagaacaact caagatggag taaaagttac aacttaagtc ctatgacatt
     7141 tttactgaat cagtgcataa tatggatgta aggaaaagct tgcaaacaat tttgcttaag
     7201 gaggcaattt ccatgaaaga aagagaacgt attttagatt tagtgaaaaa aggaatcctt
     7261 tcgactgaag aaggcttaga tttattagaa agcatggcaa ctgaaaaaga cgaaaagcaa
     7321 ataaaaaaag aagcagacaa ggtaactgct agtcataaag aaaaagatca ggcaagtcaa
     7381 ttgatcgata aacttgaaaa cggcgaagaa gaaatttctg agccagttga tccaaaggaa
     7441 agagaaagac aagatcaaga aaacttagaa aagatcttag atgaactagc aactgaagcg
     7501 aataaaactt cggcgcgtct agatgaagta aatgctcaat tcgctgacaa taaggcagca
     7561 cgcaatgaga aacaggaagc attgatgcag ctgaatacaa aagaagaatt aggcgagttg
     7621 acagaagaag aattagcaca gcgtcagacg ttagaagcag aaatcaaaga attagaagct
     7681 agtggcgata cactgttaga agaacaattg aaattagaag cagaattaaa agatatccgt
     7741 aagaatcaat ggtctgagaa aaaagaaacg tttactgata agtttgaatt gccagatgat
     7801 tggaaagatc aagcaacaga tacactgaat caagtaggcg aaaaaatgtc agaagctgga
     7861 tcacagttag gcaaattctt gaagaaaaca ttccaaactg tttcagaaac agtgaatgac
     7921 aacatggaat ggaaagatgt cagcttacgt gtccctggga ttgcgacaac gaaatttgag
     7981 catgaatttt actatgaagc tcctgcagca agtatccttg atatcaaggc agcaaacgga
     8041 aacgtgacgt taaaaacgtg ggacagcaac gatgtgaaag ttgaagctaa gatcaaactt
     8101 tacggaaaaa tgggtgctga accatttgaa gcattctctg aacggagcca gattgaggtt
     8161 aatgaagatc atatctcctt ccaaattcca aataaacgtg tgcgtgcaga ccttgttttc
     8221 tacttaccta aacgagtata cgaccacgca gctattaaac ttctaaatgg caacatcatg
     8281 attgaaacat tggaagctaa agatatttat acaaaatcta cgaatggtaa catcatcgtg
     8341 gaccaattaa ctgcaacgat gctggaagta gaaggtgtaa acggcaatat cgatattcga
     8401 aacgggaaca tcttagattc tattatcgaa acagtaaatg gtacagtgac atttggtgcg
     8461 acagcagaaa acctttctgt gtctttggta aatggtgatg tacgtcttac gattaaagaa
     8521 gataatttga aaaaagtaga agcaagttca gtgaacggaa atgtcaaagt cgctttacca
     8581 gatggcatcg gtttggaagg acatgctaaa acaagtcttg gaagtatcaa cagccgtctg
     8641 tccaattacg aagtagttcg tgagaaaaaa gaacggacaa accaaatgtt gcaattccgt
     8701 cgtgtttcag atggtgaaat cacgcaagtg caactttcaa cgacaacagg aagtatctat
     8761 ttaaaagata cagataaata aagaggttgt gaaagagatg ttcagccttg agcaataaga
     8821 agcgattttg taaaatagct tcccctattt tacaaaattg tgccttattg ccgataggct
     8881 atctcttgaa caccgtttac caagagatta tgaacctgcc aattatcgcc gagtcacaag
     8941 gaataatttc agaaaaagct cgtcacgttt tatgaatatg gtaatttgtg accgagaatt
     9001 ttgcagaaag acaccgatta agaagaaggt tgtcacaacg attttcttca tttaaaatag
     9061 tagaacagag gtgagacatg atgaaaaaaa agttgacaaa atccaataaa aatgttgttt
     9121 tgacagggac tttagcagga ataggagaat acatcgggat tgatccgact gttatccgtg
     9181 tgctttatgt attcctgagc ttagttgctt ttggaagtcc aatcatttta tacattttat
     9241 tagctttgat tatcccatct gcgcgttctg cacgaacaaa ctatggacat aacaatgcat
     9301 attatgaaaa aaacaattat cgtgaacaaa ctcgtgaaca aacaaaaaaa cgcaaagaag
     9361 cggaaaaaat cgatgatgac gaatggagtg acttttaatg tcgtactttc aacgtatttt
     9421 agttaatacg ttaacattca tatcattggc tgttcttttt cctgcgatgg tccatgtgag
     9481 cagtatttgg acggccgttc ttgccagctt cgtattgtct gtgttgaata tgtttgtcaa
     9541 accgattttg acgatactgt ccctgccctt tacattagtc acattgggat tgtttacgtt
     9601 tgttatcaat gcagcgattc ttaaaatgac ctcctttttt gtgggagcag aacattttag
     9661 tttttcttca ttcggtgcag cgttgttagt agctatcttg atgtcgcttg tcaatgtaat
     9721 tgtcagcgaa cataacagta gaaaataaaa gaaagctgtg ccgaactatc ggcgcagttt
     9781 ttttatttag aatcaggaat gcgttttcta ttacattggt aattaaataa aaacgttttt
     9841 tttaattggt agtacttttg gctagacaat gataaaatga tagagaacac tctaatagaa
     9901 atgaggtagc atgaatgtca gaagttgtaa aagttagcca attagtgaaa aaattatctt
     9961 tggaaatcgt gacaggcgac gagaaaagct tagaccgtgt catcgtaact ggggacatct
    10021 cacgcccagg actagagttg actggttatt ttaattatta ttcccataat cgtttacagc
    10081 tatttggcag taaagaaatc agttttgcgg aacggatgat cccagaagag cgtttgatca
    10141 tcatgagaag attatgtagt gaagatatgc ccgcatttat tatttcaaga ggattaccgg
    10201 ctccagaaga aatgatccag gcagcaaatg aaaatgggat agctgtactg cgttctccca
    10261 tctcaacttc tcgtttatta ggagaattat ccagttattt agatggtcgc cttgcccctc
    10321 gaacaagtgt ccacggtgtt ttagtggatg tttacggatt aggtgtactg attcaaggtg
    10381 acagtgggat aggaaaaagt gaaacagcgt tggagttgat caaacgcggt catcgattga
    10441 tcgcagacga tcgagtagat gtttatcagc aagatgaatt aacggtgatc ggagaaccgc
    10501 ctaaaatcct ggaacatcta attgaaatcc gaggaatcgg gatcattgat gtaatgaatc
    10561 tttttggtgc gagtgcggta cgtggctcta tgcaagtcca gttggctgtt tatctggaag
    10621 catgggcaaa agataagaaa tacgaccgtc tgggctctga agacacgacc gttgaaatcg
    10681 cggaagtggg cattccacaa ataaaaatcc ctgtcaaaac aggacgaaac gttgcaatca
    10741 tcattgaagt agccgcgatg aatttccgtg cacgtacaat gggctttgac gcaacgaaaa
    10801 catttgaaga aagattaagt cgcttgattg aagaaaactc aggcaacgac tagcaaggag
    10861 tgacaatatg ttagcacaaa tcaatcgagt tgcttttgag cttgtaggga ttcctatcta
    10921 ctggtatgca ttgatcatcg tatcaggtat cattatcgcc atgtggctta gcagcagaga
    10981 ggctgttcgg gtaggattaa aagcagatga tgtgacagat tttatgttgt ggggattgcc
    11041 tatctccatt attggcgctc gcttatatta tgttttattt gatcttcccc agtatatagc
    11101 tgatccaatc caaatattca atatccgttc aggaggctta gctatttatg gcggactgat
    11161 tgcaggcgcg atcacgctgt atttttttac taaatatcat tttatttctt tgtggacttt
    11221 tttagatatc gctgcaccaa gtgtattatt agcacaggca atcggtcgtt ggggaaattt
    11281 catgaaccac gaagcatatg gaccagacac gacgcgaagc ttcttagaaa gtctgcattt
    11341 accgcaattc atcattgata atatgtatat cgaagggatc tatcgtcaac caacatttct
    11401 ttatgaatca ctttggagtt tgattggatt tattttgctt ttgtttttac gaaagaaacc
    11461 acaccttttg aaaaaaggcg aagtcctttt atgttatcta atgtggtata gtttcgggcg
    11521 tttctttatt gaaggattac gtacagacag tctttacttt ttaggtgtga tccgtatttc
    11581 tcaattatta tctgctgtgt tatttgtcgg tacattgatt ctatttattt ggcgcagaaa
    11641 aaaaggaaat ttaccggatt atgaccgctc attaggaaaa aatcaacaaa ttatctgatt
    11701 tttagagcgt taaaaatgaa aaaaatttca ggagtggtaa aatgaattat agagtagcgg
    11761 ttttaggacc aggctcttgg ggtactgctt tggcacaagt cctcgccgaa aacggacatg
    11821 acgttcggat ttggggacat cgtgcttcac aagtagaaga aatcaacacc caacacacga
    11881 atcaacgtta tttaccagat cattatctac ccgattccat ccaagcattc accaatatgg
    11941 aagatgcagt aaaagacgca gatgcggtgc tattcgtcat tccaacgaaa gcaatacgtt
    12001 ctgttgcaaa agaactagtg cctaaattga atacaaagcc agtgatcatc catgcaagta
    12061 aaggattaga acagggaaca cataaaagga tctctgacat cttgttagag gaaatcccag
    12121 cagaaaaaag aaaagctgtt gttgttcttt ctggaccaag ccatgcagaa gaagtagcga
    12181 aacatgacat cactacgatc acagctgcaa gtacagatgc aaaagcagca aaatatgtac
    12241 aagagctgtt catgaataat tattttcgga tctataccaa tcctgatgtg atcggtgtag
    12301 agatgggggc agctttgaaa aatattattg ctattggtgc tggcgctatt cacggcttag
    12361 ggtttggcga taatgcaaag gcagcaatca tgacgcgcgg attagctgag atcagccgtt
    12421 taggtgtagc aatgggtgcc aacccgctga cctttatcgg attaagcgga gtaggcgatc
    12481 tagtcgttac ttgcacaagt gttcattcta ggaactggcg cgcaggcaac ttgttaggac
    12541 aagggcagcc cctagaagag gtgctagcca atatgggaat ggtcgttgaa ggtgtgaata
    12601 ctacccaagc agcgatggaa ctagcccaaa taatggaaat cgaaatgccc atcactcaaa
    12661 caatctatga agtgctgtat gaacaaaaag aaatcaaaac agccgcaaaa gaaatcatgt
    12721 tacgtgatgg gaaaaaagaa aatgaattct cattaacaat tttttgaagg agcgaatgta
    12781 tatgaaagtc aaaaaagcag ttattccagc agcaggatta ggaacaagat ttttaccagc
    12841 aacaaaagct atggcaaagg aaatgttgcc aattgttgat aaaccaacaa tccagttcat
    12901 cgttgaagaa gcgttgaaat caggaatcga agacatcttg atcgttacag gaaaagctaa
    12961 acgaccaatc gaagatcact ttgattcaaa tattgaatta gaaacaaacc taagtgaaaa
    13021 aggaaagacc gaattattga aattagtaga agaaacgaca gatgtcaact tgcatttcat
    13081 ccgtcaatct catccaaagg gtttaggaca tgccgttctt caagcaagag cctttattgg
    13141 aaatgaaccg tttgtcgtta tgctaggcga tgatatcatg gaagatgaag taccattgac
    13201 acagcaatta atcgatgatt acgaacgtac tcatgcttct acgatcgccg ttatgcaagt
    13261 tcctcatgat gaaacttcaa aatacggaat tatcaatcca ggagaagtat tggaagatgg
    13321 gttatacaat gtcaaaaact ttgttgaaaa accagatcca agcaaagcac caagtgattt
    13381 agctattatt ggacgttact tattgactcc agaaattttt gatgtattgt ccgagcaaca
    13441 accaggagcc ggaaatgaaa tccaactaac tgacgcaatc gatacattga ataaaacaca
    13501 acgcgtattt gcccgtaaat tcacaggtaa acgttacgat gtcggtgaca aatttggttt
    13561 tatgaaaaca agtattgaat atggtctaac acatccagaa gtaggtgcac ctttacgtga
    13621 gtatatcctg tctttaggta aaacattaga agcagaagga agcaagatcg atcataatcc
    13681 aaaaggaata acagaaatca aagaagaaaa gaaaaaataa cccttttttc taacgagctt
    13741 cagctgctaa ttgaataaaa agtagttgaa gctcgttttt atttgtttaa aaaacacgaa
    13801 aatgctatcc ttatagaaaa gagagacaag aagggagttt gatcatgtca ggaggagaaa
    13861 ttgcaggatt gattgcagca atcgcctttg cgatacttgt cgtttttatc gttcgtgtgt
    13921 tgttgcaagt ttctaaaact ttagaaaaag tagatcagac tgtagcagaa gcaaatacga
    13981 cgatcgaaat cgtcacaaaa gacgtagatc ttttgtctag acaagtagaa ggcttgctgg
    14041 tcaaaagtaa tgaactgctc ttggatatca atggaaaagt cgcaacgatc gatccattgt
    14101 tcacagcagt tgctgattta agtgaaagtg tatcagaact taatcattct agtaaaaaca
    14161 tcgcgacgaa agtcggcgga atcggtaaag cgacagctaa agcgacggtt gccggaaaag
    14221 tcggtgggac agcgatgaaa ttttttaaaa acaaaaaaca tactgaaacg acaggaggaa
    14281 aataaaaatg gctaaaaaag gtggattttt attaggtgca gtagtcggag gaacagccgc
    14341 agcaatcgcc gcgttattgt tagcaccaaa atctggtaaa gaacttcggg atgaattagc
    14401 tgcccaagcg gatgatttga tggatatggc ttcagattac acagatatgg cgaaagaaaa
    14461 atcgactgag ttaacaggta ttgcaaaaga aaaagcgcaa gatctttctc aacaagcagg
    14521 tgatctagca ggtagtgtca aacaaaaagt aagtgaatca gcagatgaag caggaagtga
    14581 atctgacgat atcttgttaa atcttaaaga acaatcagct gacttatcta atcgtttcaa
    14641 aaaaacggca gaagatatga aaaaacctgc agatgaatta ggaagtattg ctaaagatac
    14701 ttcagaagat atcatgattg atgtaaaaga cgctgctggt aaagcaggag atactgtcaa
    14761 tgaaggaatg tcagaagcaa aaccagttgc agatgacaca aaagaaatga tgaaaatggc
    14821 aaaagaagat gcgaaagaag ctaaaaaaga aataaaagat acaatgacag attcatctac
    14881 aaatcactaa aatcacaaat ttatagaata aaaaggtggg agcggactag ctctcacctt
    14941 ttttactctt atttttctaa aacaagtaat tcttttggtg tagtcgtgaa tggcacacag
    15001 ccatcgcttg tcacatatac acagtcttcg atacgtaccc ctactttttc aggaatatag
    15061 attcctggtt caagagagaa gcacatccct tcttcgatta ctaagtcatt tccttctaca
    15121 agggaaggga attcgtggac ggttgttccg atcccgtgac ctaaacggtg attgaaatat
    15181 tcgccgtagc catagctgtt gatcacgttt cgtgcgattt gatcaagttc acctgctgtc
    15241 acacctggac ggacggcttc ttgtgcggca agctgtgctt caagagtgat gttgtatatt
    15301 ttttcttgga agtcggttgg tttttgataa gcaattgtac gagtagcatc actgcaatag
    15361 ccattccaaa caacgccaag atcgaataac acaagttcat tcggtgaaat cttcgtgttt
    15421 cctggtgtac catgagggct tgcagcattt gctcctgcaa ggactaatgt gtcgaaagac
    15481 atttgtgaaa caccttgttt tttcaattgg tattcgattt ctgcaatgat cgcttgttca
    15541 gtcacgcctt ctttcactgc tttaaagcca atctcaaatg caacatcggc ccatttacca
    15601 gcttcgatca gtttgtcttt ttcctctaga gttttgatca gttgtagacg ttggatgatg
    15661 gaagttagat cagtgtaaga atccgttccc gtcaagatat cagagatagc atcgaagcgc
    15721 tcgacagtca aggcgttctt ttcaatccct acattgccag gctgaccata gcgagcacga
    15781 agttcttttc cgatgatctc ccaagggttt tcgctgtcta aatagccacg gacatcatac
    15841 gcccaactac tgttttgagc atcttcaact tccaaagcag gagtgaaaag gaaaggatct
    15901 tgttcaagag ggataaaaag agctagtaca cgctcatgag gatcactttc gtatccggaa
    15961 aagtaagcga tgtggccagg gtcgctgata taagccaatt cgacttggtg ttgtgccatc
    16021 cattgacgta gttcattgat ttttacgtta ttcatttggc aacctacttt ctaaattaat
    16081 tttgtattca ataattgtac cactgattta ttaaaagacc aacgttctaa aaactgaaaa
    16141 aagtaaaaat tttcatgaaa acagagataa aacggttgca attcttgaga ttatttgata
    16201 ttctatttta gaaatgtaag acgtattttc agaaaacata taaaatatga ataggagaac
    16261 acaaatggaa aagcaaacaa tcactatcta tgatgtagcg agagaggcaa atgtttcgat
    16321 ggctactgtt tcacgtgtcg taaatggcaa tccaaacgtc aaaccagcaa caagaaaaaa
    16381 agtcttggaa gttatcgatc gcttggatta tcgtccaaac gcagttgcgc gtggcttggc
    16441 aagtaagaaa acaacaacag ttggtgtaat catccctgat gtcagcaata tgttctttgc
    16501 ttctttagca cgtggtatcg atgatgtggc aacaatgtac aaatacaata ttatcttagc
    16561 aaattcagat ggcaacgacc aaaaagaagt caatgtattg aataacttac tggctaagca
    16621 agtggacggc gtgatcttca tgggtcacca tatcacagac gaaatccgtg gagaattttc
    16681 acgttcaaaa actcctgttg tattagctgg ctctattgat ccagatgaac aagtaggtag
    16741 cgtaaacatt gattatgtat ccgcaacaaa agatgcagta aacaaattgg caaaaagcgg
    16801 caataaaaaa atcgcttttg tcagtggtgc gttgatcgat cccatcaatg gacaaaatcg
    16861 tctgaaaggc tataaagaag cattaagcga aaataaccta ccatatagcg aaggattgat
    16921 ctttgaagca caatacaact tcaaagatgg tctatctcta gtcgatcgga tccataatag
    16981 cggtgcaaca gctgtctatg tttcagatga tgaattagcc atcggtattt tggatggttt
    17041 attagaccgt ggcgtgaaag taccggaaga attcgaaatc atcacaagta acaattcact
    17101 actgacagaa gtagctcgtc ctcgtttaac aagtatcaca caaccattat acgatttagg
    17161 ggcggtatct atgcgcctat tgacgaaaat gatgaataaa gaagaagtcg aagaaaaaac
    17221 gattatcttg ccttatggaa tcgaagaaaa aggatctaca aaataataaa aggacaacca
    17281 ttcagaggtt gtgtgcctgc cgtttaactc tgcgttacaa ggagcaattt caaaaaacag
    17341 catctcatgt ttgatgaata ttgtgactcg tgattcggag ttggcaggag aacaccgttt
    17401 atttgggaaa aggggttatg acaaaatttt gttataatcc tttttttgtg tcttaaaaaa
    17461 gagagaaagt ggttgaaatt atatttatcg tatataaaac aatccgtgta tagatgctgt
    17521 attttgttca ccatcatcaa aaaaataagg gaatgtgaca atactattgt cacattccct
    17581 tattactgtg tattctatta aatcaatcat ctttctcatc tgattctttg gttgcttgat
    17641 tcgctcttgc ataagctgaa gcggtgttcc agtaatctgt atagttttca tcggtaccgc
    17701 caacgccaaa cttaaaggcg cttttcttcg gtccattttt cgcccatagc gattcaactg
    17761 tttttcctgg tgtttggatg gattttttat tgacagtaac ttttccaggt gtttgaccag
    17821 taaatccaga tacttttttc ttcatcacat cagatgataa ttcgaatttc tcatcagtgc
    17881 caaaaatgtc aggttttgct tggtaagctt gattgatcag attggcaaga taattagccg
    17941 aacgtatcct tgctttgctg tccattccag tattgtcatc gtgccctgcc cagctactga
    18001 tggtaataga cggagtagag acaattagcc atgaatctcg gtattcattg gtagaaccag
    18061 tttttccgac ccaatctgct tttcctagat ttccgtttaa gcttgagatg gcatctttga
    18121 atggtgtagt gatttttgca ttgatcacac tgcgcatcat gtcattcatg atcgatgccg
    18181 ttgcttccga atagatccgg acagagttgc tttcgtgttt ataaattaca tttcctgcgt
    18241 tatctgtgat ggaatcgatg atatagcctt cttcatagac gccgccgttt gctaaagcct
    18301 gaaatccgtt ggtctgttga agcgtcgtga catctactgt acctaaagga gcagattcaa
    18361 cgccccaatt gtcatttgcc gggtagttca tctttgacaa gtagtgctcg taagcatatt
    18421 gtttcgatcc accggaattc aacacatctt gatataagtg ataagccggg atattgttgg
    18481 accattctag tgactcacgg acggtttgga aggtgttcga tccttcgtta gtagcattga
    18541 cgattttctc gccagcattt tctccttctt gccaagttgt tgcataatct gatacacggg
    18601 attctgatcc gataagcccc atgtcgattg caggtccgta aacaagaact ggtttgattg
    18661 atgagccagc ttgacgttct gtatcgaagg catggttatt ttgattctga ctgtaattcc
    18721 gtccgccaac gaatccgtag atgcggccag ttctgttatc catcaataca tttccggttt
    18781 ctacttgact gccagcacca tcatctaaca tgtatccata attttcaacg ccattttgca
    18841 atgcagcata gacatcttta tcgattgtcg aatggattgt atatccttta ttttggatcg
    18901 tttgctgtgc cttttgataa taagcgtcac tgatcgatga atcagacata tcagcgttgt
    18961 ctttttcagc caattgttgt gcaatgattc ttgttgcttc attcatcaca gtataataca
    19021 agaattctct gtcagtctgt tctgcgattt gttgtggtag aaagtctttt gtaaggtcat
    19081 aggcttttgc ttcttcgtat tgttctttcg taatttggcc ttcacgatac atactgaata
    19141 aaacaaaatc ttttctggca aggccggcag aaagatcttc ttttagcgca cctgtgttcg
    19201 tgtatggact atacgtgatc gggctttggg gtaatccagc gatataagca gcttgcggca
    19261 gagtaacatc ttttgcatct acaccaaaga ttcctcttgc agcttcctgg actccagcaa
    19321 tgttttgtcc cttattatta cgaccaaatg gtgagacatt caaatacgta gcaatgatct
    19381 catcttttga aaagtatttt tctacttgtg cagaaagaat gatctcgttt gctttacgtt
    19441 tgaatgttgt ttcatctgta aggatctgct gtttgactaa ttgctgtgtc aaagttgatc
    19501 caccagaaga gccaatacca gtagcttcag atactaatgc tcgtaaaaca gctttaggaa
    19561 cgtaaccttg gtgtttatcg aagtattcat cctctgtgct gataattgca gttttcaata
    19621 aaggtgaaat tttatcagat gaaatagtcg tgcgcatcag atctgtctgg atttttgata
    19681 tctctgtatt atctgcataa acaatctttg acgtttctgt aatgtttcct agttctgtct
    19741 gcagtgcttt tttagtcggc aattgtgtgt cttcgacgag ataggcaaaa tagccagcgc
    19801 ctattccaat acctagagct gcgaacatca aaagaagaac agtaataaaa acagtcaatg
    19861 attgaaaaac tctaaaaata atcttaggaa caaaccattt ttcttttttt cttctattct
    19921 gtttttttcg agtatttttg cgtggcaaaa gggtcacctc aactaatggt tttatttcgc
    19981 taattatagc aaataataga acaaaaaaac agacaaggct gtttttggca ggaatttctt
    20041 ttttaaagga gttaaaacgt gagaaaatga tcttttttat aaaagagttc ttaagctttt
    20101 ctttagagaa aatttcagtt actatggaga acagggcaaa gaaaaatcaa tcattttctt
    20161 tgtatatcat cttaccaata tataggaatt gttagtaaca attgcctttc aaggaggaat
    20221 ccatccatgt tattaggctc acacgtcagc atgagcggaa agaaaatgct gttaggagca
    20281 gcagaagaag cagccagtta tggatcttcg acttttatga tctatacagg cgcccctcaa
    20341 aacactagaa gaaaagccat cgaagagatg aatattgatg ccggaaaagc gtacatggca
    20401 gaaaacggtc taacaaatat cgtcgttcat gccccttata tcattaattt ggggaatacg
    20461 atcaaaccag aaatgtttcc ttttgccgtc caatttctga gagaagagat tgttcgtgcg
    20521 gaagctttag gcgctactca aattacactt cacccaggtg ctcatgtagg ggcaggaaca
    20581 gcagccggaa ttgctagaat tattgaaggc ttgaatgaag ttttgacaaa agaacagacg
    20641 gcacaaatcg ctttggagac gatggcaggt aaaggtacgg aaatcggtcg aacatttgag
    20701 gaattagcgc aaatcatcga tggagtaaaa ttgaatgaaa aattatccgt tacttttgac
    20761 acctgtcata cgaatgatgc cggttataat attaaagaag acttttcagg tgtaatggaa
    20821 gagtttgata agatcatcgg gttagatcgg cttaaagtac tccatatcaa tgattctaaa
    20881 aatccgcaag gttcgcataa agaccggcac gccaatattg gttttggaac aatcggattc
    20941 gacacgttga ataagatcgt ccatgatcca atgtttgaca atatttcgaa aatcttggaa
    21001 actccctatg tcggcaaaga taaaaagaaa gcttacgcac catatggaaa agaaatcgcc
    21061 atgttcaaag cgcaaacatt cgatccagaa gtgtttcctg agttattagc agaagaagtg
    21121 atactctaag attttctatt tgacagcagc aaaaaaggac caggcacatt gccttgtcct
    21181 tttttgttta agaaacaaag gagtactttt tgcagtcttt ttcatcatgc atcttctccc
    21241 ccctcttttt aaatttagac aaacagggta tactggtaga aaagtaaaaa aacgaaaagt
    21301 ttcacaagca agtggcaaaa cgtgattgga aggacgaaaa gatgctaaac actttaaaac
    21361 ccttgatcaa ccaactgact aagaaacata catactttat tcttggcata gccctattat
    21421 ttacaattat tcgtttgtat ttatatagta aagcaatcta tggaacagac atgtcggcaa
    21481 cctatgatga tcagttgctg attcgctatg ctcaaaatat tttggacggt aaatggctag
    21541 gggagtatac gactacaaca ctttccaaag gaatctccta cagcttattt ttagtacttg
    21601 cacataaact gcatttttct tatgcagttt tattcggcat gttcagtatt tttgccagta
    21661 tattggttgc actcgcgttt aaaccgatcg tgaagaacaa gtatcttctt ttaagtatct
    21721 atttttattt tttgttttct ccgattagtt ttacacatga atattccact cgaacgtatc
    21781 gtaatgccct cgtgattccg gctgttgaga tcattatcgc ttgtttatta gctatttatt
    21841 accgcaaata cggttctagc agaaaattgc tgccatggtt tttagggctt tctgtcgctt
    21901 ttcctttttt ttggttcatt cgcgaagatt ctatctggct tcttcctttt actgtagtag
    21961 ctttgggtat cagtttcgtc cagatcttgc tagggcaaga cttttctttc aaagaagtaa
    22021 agaagttaaa aaaacgactc tttcttctct cgaaacggga atggctgaaa ctttcattgt
    22081 ttttacttcc tatagttggc ttttccatcg tctatcagtg gatcgaatat aaaaatgaga
    22141 caacatacgg tattgcaacc atcaatgacc gttcgagtgg agaatttgga cgtttgatga
    22201 aacaactgat ccgaatagat gatgaaacga acttgaataa agaaaacagt gagatatggg
    22261 tctcacatgc tgcactaaat aaagcattag ccgcatctcc tacattccat actgtttcta
    22321 cccgtattga cgaattatat acgactcacg cgtggactca aggtggaaaa gtaaaagaat
    22381 tacccggaga tatcatcttt tgggcattgc gagatgtagt aagcgaatct ggttattacc
    22441 aaaacaatgc aaaagaaacc aatgattttt ggaaaaaagt caaccaagaa ttagaagaag
    22501 cctatcacaa gggaaccatc aaaaagaaaa aagagttcta tctgatggga tcaggagatg
    22561 gaaagacgat gagtgatctg ccaatcgtat tcgatttcct tcaatcagga tacgcttatg
    22621 catggcgata taaagatttc catcaaggag gaaattatag tttcggaacg caagaacaag
    22681 tcaaacaggc tcaagaatta ttaaaccttc cgctaatgga gaattggatc gatagtaatg
    22741 aaccgagaac acctaacttc caattaacaa aatcagcaaa agtcgcgaat gtcatcataa
    22801 aaatttacca agttctggct attcccgtgc tgattgcttc aatagcaggg atattactta
    22861 tgatactagg aaccgtattt gcaaaagctg atcgtgccta ttatagttcc tctttgattg
    22921 tggtcaccgg attaatactg acacaattgg tttttctttt cggtgtctca tggttttgtt
    22981 cttatgcgcc agaaaaaaag gactattttt taagtgtcta cactggtgca ggcgtgccat
    23041 taacgcaatt catcgtagta acaagcatgc tagttttcag taaattaaat ctgaaaaaag
    23101 ctttatcttc taaataaaaa gacccttggt agctagattt ttaacctaac tatcaagggt
    23161 ctttgtgtgg aaaaatcagt ttttattcga tttcttctac cgtttcctgc aaaagctgtt
    23221 catatttttt gtttccttta tataaatcac caatcgtcca tgccaacagg agaaggacaa
    23281 gaacaatgat tacataagcg atgtgatcag ccaatgccag ttgagcggaa gtggcttgct
    23341 cgccgaagaa actttcgatc tgtcctggta atcctcgtaa gttcaagtaa gttagactaa
    23401 tcacagagaa ccagccaaga agttttacga tgaatgaatt tttaaatcgg ctacccatct
    23461 ccgcttggct gttggtcatc atcagtaaag gaatcatcga aaatggcaac gcaaaagcca
    23521 agaacacctg ggattcattc attaagttat tcaaagcggt atgttcttcg atcgttcctt
    23581 ttgtacttgt aaacaaaaca caaatcaata caggaatcac agaaagcaat cgtgtgacca
    23641 gtcttcttag ccaaatcggg atacgcatat ggatgaatcc ttccatgatg acttgaccgg
    23701 tcaacgtacc agtaatagta gagttctggc cagaagcaag aagtgctaca gcaaataaag
    23761 tggacaagat accagatttt gctacagcaa tcaaaatacc attgctcata gctgatggat
    23821 cagataaagc ttcatacaga ccaaaaaagg aaggatcttg gacagaaccg gttttgaaga
    23881 cagcgacacc catgatcaat agcagtgcat tgactagaaa agccattgac aactggatat
    23941 tagaatccca agtagaaaaa cgtacagctt gtgctacatc ttcttcgttt tggtgatcga
    24001 tcttccgagt ttgtgaaact gctgaatgaa gatataagtt atgcggcatg actgttgcac
    24061 caataatccc taaagctcca gttagcggag tttcgccacc aatgacaggt gagtctgaga
    24121 atgtatggct gttagggata aagccagcaa ataaactgtg ccaatctggg ttagacaagc
    24181 caacttgata gacaaaaacg aataaaatga cgaaaattaa gcagacaacc agtgcttcga
    24241 tcttacgaaa cccgactttt gtcagtagta agagaacgaa aacatctaat acagtaataa
    24301 agacggcaat caccaatgga atatcaaaaa gtaagtagag ggcgattgct gcaccaatta
    24361 cttctgcaat atctgttgcc ataatggcta attcagtcaa gatccataaa atgacaccta
    24421 atgtcttact tgtgcgtgct cggattgcct gtgccaaatc catttggctc acgattccta
    24481 attttgctgc catatactgt aaaagcatag caataagact agacatcaaa ataactgaca
    24541 tcaataaata ttggaagttt tgtcctccgg tgatcgaagt agaccagtta cccggatcca
    24601 tatacccaac tgctactaac gcaccaggtc cagagtaagc aagaagtgtt ttaaaaaaac
    24661 ttttcccctt tggtacatca actgttccat ttacttcagc taaagataaa ccatttgtat
    24721 gttcaataag tcgatgctta tgaaattgtt tattggattg attattcaag aaaaaaccac
    24781 ctttcctatt tatctacgat tacattctac acctcaaaaa gaaaaaatta aagaaaaaga
    24841 ttcggtatgc ctaatttatt ttcgtggcgt gtcttgctag atgacgaaag aaattttgtt
    24901 ctcctctaaa aaaaccgcta ttttttgcat gaaactaaag aatacgcctt tcttttttca
    24961 ataaacttta gtaaaataag tcattgagag caattcggaa atagaaagga gagatttata
    25021 tgttatcaac aggtattgtc gtacttattg ctattattgc tttgctaatc ggcgcagtag
    25081 gtggcttttt cctagcgcgt aaatatatga aagactattt agaaaaaaat cctcccgtta
    25141 atgaggacat gttgcgatca atgatgatgt caatggggca aaaaccatct gagaagaaaa
    25201 ttcgccagat gatgcagcaa atgaagaacc aagggaaaaa ataggttctg acccaacgac
    25261 tttttacggg ctgatttttt cagcccgttt tttatttcgt aatttgttag actattcaga
    25321 ttattcagca aaaaagacaa gcacagacaa tcctaggctc ttgataaaga caaaggagga
    25381 gtaaaatgtc gatttttaga aaattaggct ggttttttcg tcaggaaaag aaacattatt
    25441 taattggagt tttctcgcta ttcgcagtgg ccttggtaca gttagttcct ccaaaagtga
    25501 ttgggatcat cattgatgag attgccgata aaaatttatc aataaagatc attttcgggt
    25561 ggatcttcct attgattatc gctgcttttt tacaatatct ttttcgttat atctggcgga
    25621 cgaatatttg gggaagtgca gctcggttgg agaaagaact tcggcagcag ttattcaaac
    25681 attttacaga aatggacaat ctcttttacc aaaaatatcg gactggggat ttgatggctc
    25741 atgcaacgaa tgatttgaat gcgatccaga atgtagcagg agctggtatc ttgacctttg
    25801 cagattcagt gattacgggc ggaacaacga tcattgcgat gattttgttt attgattggc
    25861 gcttaacact gattgcgatg attcctctgc ctttgctggc catcacctca cgagtgctgg
    25921 gatcgaaact ccatgatgct tttcgtgatt cgcaagaagc tttttctagt atcaatgata
    25981 agacacaaga aagtatcact gggatcaaag tcattaaaac atttggacaa gaaaaagaag
    26041 atctagcgga ttttgaagaa aagattgaag atgcaattat taaaaacaaa cgagtcaatt
    26101 ttttagactc cttatttgat ccattcatta ccttgattat cggtctgtct tatgttgcaa
    26161 caattatttt aggtggaaag tatgtcttgg aaggcacgat ctcgatcggg cagatggtct
    26221 catttattag ttatatcggt atgcttgttt ggccgatgtt tgcgattggt cgattgttca
    26281 acgtattgga aagaggaaat gcttcttatg atcgcgtcaa tgaacttttg catgaaaaaa
    26341 cgcatatcgt ggaacaactg ggtgcagtgc agaccccggc aaaaggcgag ctgaatgtag
    26401 ctatttcctc ttttaattat ctgaaagaag aatcaccagc attagaacaa atcaagtttg
    26461 caatccaaga aggagaaaca ttaggtattg ttgggaaaac tggtgcggga aaaacgacga
    26521 tcatcaaatt actgatgcgt gaatacgatg attatcaagg gaaaatccaa tttggcggac
    26581 gttcgatcaa agaatattca ttgaatgctt tattaggatc aatcggctat gtgcctcagg
    26641 atcacttctt gttttcgatg accattcgag ataatatccg tttttctaat ccaaaacttt
    26701 cagaagaaga agtaaagcat gcggcttctt tagcatttat caacaatgaa atcaaggaat
    26761 taccaaaagg ttatgatacc ttagttggag aacgtggagt ctctttatct ggcggacaga
    26821 aacaaaggat ttcgattgct cgagcattga tcaaaaatcc tgaattatta atgttagatg
    26881 atgcactttc tgcagtagat gccaaaacag aagaagctat cttgcataat ttaaaagaaa
    26941 taagaaaaga gaaaacaact atcattgcag ctcacc
//
LOCUS       DAMYXM010000029        26918 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.030, whole genome shotgun sequence.
ACCESSION   DAMYXM010000029 DAMYXM010000000
VERSION     DAMYXM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.030"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            85..1089
                     /locus_tag="PNS21_001480"
     CDS             85..1089
                     /locus_tag="PNS21_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5159193.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1090..2034
                     /locus_tag="PNS21_001481"
     CDS             1090..2034
                     /locus_tag="PNS21_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5159194.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2034..2996
                     /locus_tag="PNS21_001482"
     CDS             2034..2996
                     /locus_tag="PNS21_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5159195.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3023..3937
                     /locus_tag="PNS21_001483"
     CDS             3023..3937
                     /locus_tag="PNS21_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5159196.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3963..5744
                     /locus_tag="PNS21_001484"
     CDS             3963..5744
                     /locus_tag="PNS21_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI5159197.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6092..6778
                     /locus_tag="PNS21_001485"
     CDS             6092..6778
                     /locus_tag="PNS21_001485"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDI5159198.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6798..10379
                     /gene="smc"
                     /locus_tag="PNS21_001486"
     CDS             6798..10379
                     /gene="smc"
                     /locus_tag="PNS21_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDI5159199.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10388..11197
                     /locus_tag="PNS21_001487"
     CDS             10388..11197
                     /locus_tag="PNS21_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDI5159200.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11208..12206
                     /gene="ftsY"
                     /locus_tag="PNS21_001488"
     CDS             11208..12206
                     /gene="ftsY"
                     /locus_tag="PNS21_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDI5159201.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12393..14660
                     /gene="gshAB"
                     /locus_tag="PNS21_001489"
     CDS             12393..14660
                     /gene="gshAB"
                     /locus_tag="PNS21_001489"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDI5159202.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKDR
                     RLTMNVLKLLYPEVF"
     gene            complement(14749..15201)
                     /locus_tag="PNS21_001490"
     CDS             complement(14749..15201)
                     /locus_tag="PNS21_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HDI5159203.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15351..16301
                     /locus_tag="PNS21_001491"
     CDS             15351..16301
                     /locus_tag="PNS21_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDI5159204.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16294..17253
                     /locus_tag="PNS21_001492"
     CDS             16294..17253
                     /locus_tag="PNS21_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDI5159205.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17250..18005
                     /locus_tag="PNS21_001493"
     CDS             17250..18005
                     /locus_tag="PNS21_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI5159206.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18028..18981
                     /locus_tag="PNS21_001494"
     CDS             18028..18981
                     /locus_tag="PNS21_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI5159207.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19625..19921)
                     /locus_tag="PNS21_001495"
     CDS             complement(19625..19921)
                     /locus_tag="PNS21_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HDI5159208.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20276..20632
                     /locus_tag="PNS21_001496"
     CDS             20276..20632
                     /locus_tag="PNS21_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDI5159209.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20642..21541
                     /locus_tag="PNS21_001497"
     CDS             20642..21541
                     /locus_tag="PNS21_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDI5159210.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            22058..22789
                     /locus_tag="PNS21_001498"
     CDS             22058..22789
                     /locus_tag="PNS21_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDI5159211.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            22934..24961
                     /locus_tag="PNS21_001499"
     CDS             22934..24961
                     /locus_tag="PNS21_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDI5159212.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            25069..26166
                     /locus_tag="PNS21_001500"
     CDS             25069..26166
                     /locus_tag="PNS21_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDI5159213.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            26473..>26918
                     /locus_tag="PNS21_001501"
     CDS             26473..>26918
                     /locus_tag="PNS21_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5159214.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYVSVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT         9022 a         4661 c         5685 g         7550 t
ORIGIN      
        1 ctttgttata aaacgattta ttctgtgtaa tgtccttttt tattaagaaa aacttccatt
       61 taggaagcag agaggggtat tcgattgtcc gataaccaat tattagaagt tcagaaccta
      121 catacaggat tccgactcaa agatgactat tacgatgcag tagatgacgt ttcatttact
      181 ttagacaaaa acgagatttt agcaatcgtt ggggaatctg ggtgcgggaa aagtaccttg
      241 gctactacga ttatgggatt acttgatcct aataacacaa aaattacagg tgagatttta
      301 tataacgatc tgaatttaat cgacctgaac gaaacgctct ataataaaat ccgcggaaat
      361 gatatcggaa tgattttcca agatccgttg tctgcattaa atcctctcat gagaatcgaa
      421 gaccagatca aagaaggact ttcctatcac acaaagatga atgcagaaca gcgtcaagct
      481 cgtgcactag aattgctaga acaagtaggg attcctaatc ctgcgagagt tggacgccaa
      541 tatccccatg aactatcagg cggtatgaga caacgtgtca tcatcgcaat cgcaatcgcc
      601 tgcaaacctc caatcgtcat tgcagatgaa ccgacgacag ccctagatgt aaccatccaa
      661 gcacagattc ttgatttact gaaagacctt caggaagaaa caaaaacagg gatcatcttg
      721 atcactcatg acttaggagt cgtagcagaa atggctgatc gagtggcagt gatgtatggc
      781 ggacagttcg ttgaagtagc aggtgtaaaa gaactgtttg atcatcctaa gcatccttat
      841 acacgctctt tactgaactc tataccacaa gaaggaaatc atgatgcaga actccatgtg
      901 atcgaaggtg tcgttccttc tctgaaaaac atgcctcgta aaggttgtcg gtttgcacca
      961 cgtattccat ggataccagc aagcgatcat gaagaagagc ctatccttca tgaagtcgca
     1021 ccgaaccact tggtacgttg ctcctgctat aagcatttcc actttagaga cgaaaaagga
     1081 gaggtgtaga tgacagaatt aatcacaatc aaagacctga aagttcatta ccctattcgc
     1141 agtggattct tcaatcgagt aacggatcat gtatatgctg tagatggtgt ggattttatt
     1201 atcgaaaaag gaaagacata tggtttagtg ggagagtctg gctctggaaa atctactacg
     1261 ggaaaagccg ttgtgggatt agaaaaggta acagccggac aaatcatgta tgaaggaaaa
     1321 gatgtaacga agcgaagcaa ccgcaaaaaa atgggttata acaaggatgt gcagatgatc
     1381 ttccaagatt caatgtccag tttgaatcct aaaaagcgag tgttggatat cattgctgaa
     1441 cccatccgaa attttgaacg gttaagcgat caagaagaaa agaaaaaggt aaaaggactg
     1501 ctagatatcg ttgggatgcc ggaagatgcg ctatataaat atcctcatga attttcaggc
     1561 ggacaacgtc aacgtctagg agtagcccgt gctgttgcta ccaatccaaa actgatcgta
     1621 gccgatgaac ctgtatctgc tctggatcta tctgttcaag cgcaagtatt gaatttcatg
     1681 aaacgaatcc agcaagaata cggcctcagc tatttattca tttcacatga tttaggtgta
     1741 gtaaaacaca tgtgcgacaa tatcgcaatt atgtacaaag gtcgatttgt agaaatcggt
     1801 acaagagaag acatttataa tgacccgcga catatctata cgaaacgtct gctttcagct
     1861 attccgcaaa tcgatgtaga aaatagggaa agccacaaag aaaatcgacg aagagtagaa
     1921 caagagtata tccaaaacca aaaagaatac tatgatgcga ctggtcgagt ctacgatttg
     1981 cgtacgatca caactacaca caaagtggcg ttgaaagatg gaggtgcaag ctaatgtgga
     2041 aaacgattct gcgacgtgtt ttattgatga tcccgcaagt tattatttta agtgttctga
     2101 tcttcttatt agcccaagcg atgccggggg atccatttac cggtttgatc aatccaaatc
     2161 aggatccggc agtgatcgaa cagatgcgac aagcagctgg cttgaatgac ccatggtatg
     2221 tccagtactt ccgttggatc ggaaatgctt tgcaaggcga ttttggtcaa agtttcttat
     2281 acaaacgctc tgttgctgac ttgatcggcg aacggatcat caacacttta tatctatcga
     2341 tcttgacagt tgtccttact tatttgattg ctgtgcctct ggggatgctt gctggccggt
     2401 atcaaaattc aatcttggat aagattgtcg taatctacaa cttctttagt tttgctgttc
     2461 ctttatttat cttcggcttg atcatgctat ttgtttttgg ttatcgttta ggctggttcc
     2521 caacaagtgg atcacaaaca ctaggtttgc aaggtggatt tatcactcaa tggatagacc
     2581 gtttgaaata tattctgtta ccatcaatta cccaagcatt tttggcgact gccgtcacaa
     2641 tccaatatct gcgaagtgaa gtaatcgatt cgaaatcgct cgattttgtc agaacagccc
     2701 gttccaaagg ggtaccgacg aataaagtat tcagtcgaca tattttccga aatgcagcgt
     2761 tgccgatggc ttctcaaatg gggtatgaaa tcacttcgtt gattgctggt tctgtagtta
     2821 tcgaaaagat ttttggttat ccgggagtcg gaaaactatt tattgattcc atcggccaac
     2881 gggactatac cgtgatcaca tccttagtat tgattttagg aatcgcgacg ctagtgggga
     2941 cattactttc agatatcatc atgagtatcg ttgacccgcg tattcggatt caataaaacc
     3001 aatcagaaag gagaatggat cgatgagtga aaaaaacaat acagcagttc aagaaagtat
     3061 tcctcctatg gggatacgaa tgattgctcg agaatttaaa aaagataaaa ttgctatttt
     3121 ttcattaggt ttgctagtgg ttttattatt agtgatcttt atcggtgcga tgctgaccga
     3181 tcaagacaaa gtgatgtatg ttagtatctt cgacaaatac gctgcaccag gaacagtttc
     3241 gacacaagga caaaagttca ttctgggagc agatgaaggc ggacgtgatg tattaggaca
     3301 attgatcata ggtgctagaa actctgttac gatcggattt gcgatcacga tccttacttc
     3361 gatcatcggg gttggattag ggatcatctc tggctactac ggagggatca ttgacaacat
     3421 cttgatgcgg atcgttgatt ttatcatgat tctaccgatc atgctgatca ttatcgtctt
     3481 cgtatcgatt atttccagat acaatgtctg gtcctttata ttcatcatga gtgcatttta
     3541 ttgggtagct aaagcccgat tattccgaag caagacatta tctgaagcaa gaagagatta
     3601 tgtcagtgcc tcaaaaacaa tgggaaccaa tgattttaaa atcatgttcc gcgaaatcat
     3661 gccgaatcta agttcattaa tcattactaa tttgacgatg aactttgcgg caaacatcgg
     3721 tatcgagaca actttgactt tcttaggttt cggcttgccg gcaaatgtac ctagtttagg
     3781 tacattgatc ggttatgcaa gtaacggaga agtcttagtg aatcggcaat ggatctggtt
     3841 acccgcatca atccttattt tagttttgat gttgagtatc aattatgttg gacaagcatt
     3901 caagcgttct gcagatgcgc gccaacggtt aggctagtat agaagcgaag ggggaaatag
     3961 gaatgaagaa aacgattttt ggatttgcag cgctgatgtc tgctgtcgtt cttgcagcat
     4021 gcgggggcgg aggtacagat acctctggaa acaactctgg aggcaacggc aataacaaag
     4081 aggaattaac ttttccaatt gcaacaaaaa ataatgatga tgcaatcgaa gacggcgaat
     4141 tagaagctgc ggtcgcaacg gacagtcagt ttaaaggatt gttccagtgg gagttttacc
     4201 aagattcata tgatgcagct tttatggctc cttctcatga accgctattt gcaaacgatg
     4261 acgatttcag aatcacagac gacggagctg caacgcttga tctagatgaa gatacgaaaa
     4321 aagcgacaat caaagtaaaa gacaacgtca aatggtcaga tggtgaagat gtaacggcgg
     4381 acgatgtgat tttcccttac gaaatcattg ggaataaaga ttacactggt atccgttatg
     4441 acgacacttt ccgtaacatc gtaggtatgg aagaatatca tgatggaagt gccgacacaa
     4501 tctccggaat caaaaaaata gacgacaaaa cagttgaaat cgaatataaa gaagtaaacc
     4561 caagcatgct tcaatcgggt ggtggtatct ggtcatatgc agcaccaaaa catacgttag
     4621 aaggtgtagc aatcaaagat atggaatcaa gcgatcaagt acgtaaaaat ccagtgactt
     4681 ttggccctta ttacatgagt aatatcgttg caggagaatc agtagaatac ttgccaaacg
     4741 agcactactg gaatggtacg cctaaattga aaaagatcac gatgaaatct gtaccgatgg
     4801 caagtgcaac tgaagcattg aactctaaat tatatgatat gattttccaa atgccaacag
     4861 atacttacga tacctataaa gaaattgctg gctatacaaa cttaggccgt gaacaaactt
     4921 cttatactta tctaggattc aagttaggta aatggaatgc agatacaaag agtgtggaat
     4981 acaatccaga ttcaaaaatg gctgataaat cattacgtca agcaatgggc tatgcattag
     5041 ataataatgc agtaggtgaa cgtttctatg caggtttgag aagcaatgcg acttcattga
     5101 ttcctccagt attccagtca taccatgatg atgagatgaa aggattcaca caagatctag
     5161 acaaagcgaa aaaactatta gatgatgctg gctacaaaga tgtggatgac gatggtttaa
     5221 gagaagacaa agatggaaat aaactaacaa tcaactttgc ttcgatgtcc ggtggggaaa
     5281 cagcgcaacc attggcagat tactatatcc aacaatggaa agaaattggt ttggatgtcc
     5341 aatacacaac aggtcgtctg atcgaattca attctttcta tgataaatta gaaaacgatg
     5401 atcctgaaat tgatgtttac caagctgctt ggaatacagg aactgatcca aatccaagtg
     5461 gactatgggg accaaatgct gcgttcaact acactcgtta tgaatcagaa gaaaatacaa
     5521 aattgatcaa cgatatcagt tcaagcaaag catttgatga agagtaccaa aaagaagcat
     5581 atcaaaaatg gcaacaatat gcatttgaag aagcatttgc gattccaacg ttgttccgta
     5641 atgaagtatt gccagtcaac gaccgtgtca aagactggga ttggtcttat gatgtaccaa
     5701 acccatgggc aaatgtttct gttacagcag attcacgttc ataatcacta aaaatgaccg
     5761 aaaaagggcg atgtcctttt tcggtttttt gatatttttc tgtttatttc tctaatattc
     5821 aggtcaaggc gctgatttca tgacttctcc aataaacggt gttcaaagtc cagtttctcg
     5881 gtattaagtc aaatttatgc aaaaatatgg aaagccattt tcacaaaatt ttttcttcat
     5941 actcgaaact aaacgaattt ttcacaactt ctcgtcaatc actaggatta ttaaagcttt
     6001 acttaccttt tgaccgaacc atttcaaatt tgacaaaaaa ccagtataat aaggaagtat
     6061 cgaaaaaata tccataaaag gaatgtggaa aatggacaac cagctgacaa aagaactaaa
     6121 agaaaactat aacatcgttt ttcacgatct aaatttgctt gaacaagctt ttacccattc
     6181 atcctatgtg aatgagcatc gcaatctgca attatcggat aatgagcgac ttgaattttt
     6241 aggagatgcc gttttagaac tgatggtttc tcagtattta ttccgtttat atcctcattt
     6301 gccagaagga aaattgacaa aaacacgagc tgcgatcgtt cgcgaagaca gcttgtcgaa
     6361 atttgccaaa gaatgtcact ttgatcaata tgttttgttg ggcaagggag aagagaattc
     6421 aggaggacgt acgcgaccag cattgctttg cgacctattc gaagcttttt taggcgcgtt
     6481 atatcttgac cagggattcg acacgaccca tgcattcatc gaaaaagtga tttttcctaa
     6541 agtcaaagca ggtgcttttt cacatgagat ggatcataaa acaaaactac aagaagtctt
     6601 gcaaaaatca ggtgatgtat caatcgaata ccgattgatc aatgaagaag gtccagctca
     6661 tgaacgagta ttttggatcg aagtttatgt agatgatcaa ttgattggca ctggacaagg
     6721 gaaatcgaaa aaattagcag aacaagctgc ggcggaaaat gcattggcag cactgtaaaa
     6781 gtaagtgagg gaaagctttg tatttgaaac gaattgaaat agccggtttt aaatcatttg
     6841 cagacaagac agtcattgat tttgaaaata gcgtcacagc agtggttgga ccaaatggca
     6901 gcggaaaaag caatattaca gaggctatcc gctgggtatt aggagaacaa tctgctaaaa
     6961 gtctgcgagg cggaaaaatg ccggatatca tctttgccgg ttccgatacc cgtaagcagc
     7021 tgaatattgc agaagtcaca gtgattttag acaatagtga tcattatctg ccattggagt
     7081 acaatgagat cagtgtaacc cgccgttatc gacggactgg ggaaagcgag tttttcatca
     7141 ataaacaaag ctgcagatta aaagatatcc aagaattgtt cttagattct ggattaggaa
     7201 aagaatcgtt ctcaatcatt tctcaaggaa aagtagaagc gattttcagt agtaaaccgg
     7261 aagaccgtcg tgggatcttt gaagaagcag ctggtgtctt gaaatacaag cagcggaaaa
     7321 agaaagcaga acagaagctt tttgaaacag aagacaacct cagccgcgtc caagacatca
     7381 tccacgaatt ggaagaacag ttaactcctt tggccgccca gagtgaggca gcaaaggagt
     7441 ttttacggct aaaagaaaca ctgactcaaa ccgatgtctc tttgatggtt gctgaaatca
     7501 agacagcaaa aaaagattgg gacaacaaac aagcacagct tgcgaaattc aatctagaat
     7561 taggcaaact atctgagtcg atccaagaac aagaatccat tttggcgaag cagcgtaaag
     7621 aaaatgcgca agccgatcgt ctgatcgaaa aaaatcagca agtattatta gatctttcgg
     7681 aaaaactgaa gcagacagaa ggacaaaaag atgttttgca ggagcggaca aagcatacac
     7741 aaaaaagctc gcaagaatat caaacttcat tagcagaagc acaaaaaaag gttaaacatt
     7801 ttgaaaagct tcaagaaagc ttgatgaaag cagcagcaga aaaagaaaca gagatccaaa
     7861 aagctgaagc aaatctcata aaaacacagc aagaattaga aaaataccaa aaatcaacga
     7921 aagaattatt ggcagaatta agagatcaat atgttgattt gatgcaagaa caagcagctg
     7981 taggaaacga actgaagtat ctagaacgtc aatatataca agaaacagca aaaagcaagc
     8041 agacgttagc caagcaatcg gaagtagaag catcagtaga ccgattgatc ttacaaaaag
     8101 aagaattgac acaaaagcag gcgcagctaa aatcatcttt aactgaaaca aaagagaagt
     8161 tagaaatgat ccaacaaaac gggaaaaagt tccaagaagc attagcaaaa gaacagccta
     8221 aaatgtatca attaatgaat caagtccagc agctacgtgc tcgtaagaaa agccttcaag
     8281 aaatccaaga aaactatttt ggtttttacc aaggggttcg attggtcttg cagcataaac
     8341 agcagctttc gggtattgtc ggagcagtgg ctgaactgat cgatgttcct gcagatttca
     8401 cgttagctat cgaagcagct ttaggtgggg cagcccagca tgtcatcgtt gaaaatgaaa
     8461 aagatgctcg gcaagccatc acgtatttga aacagcaaag aggaggaaga gcaacattcc
     8521 ttccactgac gacaatcaaa ccaagacaat taccggctca tatattgaca caagcagcag
     8581 ctgtagaagg gtttataggg attgccagtg aacaagtcag ttatcccgat caaatccaaa
     8641 ctgtggttca aaacctgcta ggaacgatcc ttttagccaa agacttaact agtgccaatg
     8701 caatcgcgca aacgatccgc tatcaatatc gagtggtttc cttagaaggt gatgtgatga
     8761 atgccggagg atccatgaca ggtggagcga ataaacgagg aaaccaaggc agtttgtttg
     8821 tacaaaatca agaattgaaa cagttgacta gtgaatttga agaagcagat aaacaactcc
     8881 aagcacaaga aaaaaaagtt caggagctgc aacaagaaac agctcgttta gccgaagaac
     8941 aagaagtttt gcgtacacga ggcgaacaat tacgttttga agaacaagaa gcgacaaatc
     9001 agctacaaaa catcataaat gaacttgaac gttttgaaaa agaaaagcaa atctcaactt
     9061 ttgagacacg tgaactgcag cagtttatcg aagattacga aaagcaaacg aacgaattga
     9121 aagacaaaca aacagatctt gaatcgcaac gacaaaaaat cgacgaagaa atcaaatcgt
     9181 taagtcaaga aagcgaccag atggaagcca gaagagcaca agtacaaagt caaaaagcac
     9241 aagaacaagc agatcttgct gtcttgaaag agcagtttaa tcatctgcag atccagctcc
     9301 gcggtgcccg agtccaaaaa gcagaggcaa ccgaacgtca agaagcgata gaaaagcagt
     9361 tggcgacatt gacagcagac ttttctgatc atgaagtcac agaagaaagt ttggaaaaac
     9421 aaattaacga attatctgct cagcgagaaa cattaaaagc cgagttggca aaagccaaag
     9481 agcagcgaga tagaaggcaa aaggagatcg ataagcttga agcagtcttg gcagagcgta
     9541 atcgtgagca aaaagcacga ctgtcggaac aatccaaatt agaagtccaa aaagaccgag
     9601 ctgaaatgat tttagataat catttgacct atctgcagtc agaatatcag ctgacatttg
     9661 agaaagcttc ccaagactat caagaaacaa ccgatatcga agacagtaga acaaaggttt
     9721 catccttaaa agaacagatt gaaaaactgg gaccagtaaa cttaaattca atcgaacaat
     9781 acgaacaagt cagtgaaaga catacattct tagcaacaca gcgagatgat ctgttagcag
     9841 cgaaaaacca attgtttgaa acgatggatg agatggatga tgaagtgcgg acaagattca
     9901 aagaagtatt tgaagctatc cgtcaagaat tcaaaattgt ctttccaaat atgtttggcg
     9961 gcggtaaagc tgaattagta ctgacggaac caactgattt gctgaaaaca ggtattgaga
    10021 ttgaagtaca acctcctggc aaaaaactgc aaagtctaag tttgctttct ggaggagaac
    10081 gagcacttac agccatcgcg ttgctctttt ccattatccg tgtctgtcct gttccgtttt
    10141 gtatcttaga tgaggtagaa gcagcgttag atgaagccaa cgtgaaacgt tttggtcgtt
    10201 acttgagcga tttccaagat gacacacaat ttatcgtagt cacacaccgc aaaggaacga
    10261 tggtcgcagc agatgtactg tacggagtga cgatgcagga atccggtgta tcaaaaatcg
    10321 tttctgtacg gatggaagat atcaatgaag aggcgaaaat caacgaaacg aaagtgtagg
    10381 aacgaaaatg atcaaattag tagcaatcga tttggacggt acattattaa atgaacaaaa
    10441 gctgatttca gatgagaata aacaagcatt agcacaagca aaacagcaag gggtcaagat
    10501 cgttctatgt acaggacgcc ccttagctgc gatggcgcat tatctgcaag aattaggact
    10561 ggtagatgaa ggtgactaca gcatcacttt caacggcggt ttggtacaaa aaaatgatac
    10621 tggtgaaatc atggaaaaga aagtgatgga agtatcagat atccaacgct tatacaaact
    10681 ggctcagcaa ttggatctgc cactagatgt tttgtctgac aatgtggttt tacagctgcc
    10741 tagtgcccca aggaagcaat ccttgtacaa tgtgttgaat aagctgctgc aattccaacc
    10801 agcggatata agcgatatta cagatgaatt tgtgcttaat aaggcagtaa tcgcttatga
    10861 ccaagaagaa ttagatccta aaatcaaaga aataccagca gaattccatg aaatgtatga
    10921 gatcatcaaa acaagaagta tgcttttgga atttatgcca aaaggtgtta caaaagctta
    10981 tggtatttct ttattggcaa aagatcttgg tttgaaacaa gaagaaatca tggcgatcgg
    11041 tgatgaggaa aatgatttac cgatgatcca atatgctgga ctaggcgtag cgatggagaa
    11101 tgctgtgcct ttcgtcaaag aagcagcaga tttcgtgaca gccagcaatg aagaaaatgg
    11161 cgtagccaaa gtgatccaaa aatttgtatt agagtagggg gagtcaaatg ggattttttg
    11221 ataaaatcaa gcgtgctttt actggcgaag agaaaacaga agaagttgaa gtacaggaga
    11281 agtacgacaa gggtttagaa aagacgcgta aaacatttgg tgaacggatg aatgagttgt
    11341 ttgccaattt tcgaacagtc gatgaagact tttttgaaga attagaagaa acacttatcg
    11401 gtgcagatgt aggttttgaa acagcgatac gaatcaccga agcattacgt caagaagtga
    11461 aactcagaaa tgcgaaaaaa tcggcagaag tccaaaatgc gatcatcgag aaacttgtgg
    11521 atctctatga agcggaagga atcaatgaag taaatgaact aaatatccaa gaagacggac
    11581 taacagttat tttgtttgtc ggtgttaatg gtgtagggaa aacaacaagt attggtaaac
    11641 ttgcccatca gttcaaaaac gaaggtaaaa aagtattgat ggcagcagca gataccttcc
    11701 gcgctggagc aatcgaccag ttagttgtct ggggcgaacg cgctggtgtt gaagttgttc
    11761 gtggcaatgc aggaggcgat ccagcagctg tcgtattcga tgctttggaa agagcaaaga
    11821 cagagcaggc agatgtttta ctagttgata ctgcaggccg actgcaaaat aaagtgaact
    11881 tgatgaacga attagaaaaa ataaaacgag taatccagcg ggaattacca gaagcacccc
    11941 aagaaacact tcttgtatta gatgcaacca ctggtcaaaa cgcgatggtt caagcaaaac
    12001 aattcaaaga aacaacagat gtgaccggtc ttgttttgac aaaattagat ggtacagcaa
    12061 agggcggtat cgtacttgct atccgtaatg aattgcattt accagtgaaa ttggtcggtc
    12121 ttggtgaagg aatcgatgac ttggagccgt ttgatccaaa tgattttgtc gttggattgt
    12181 tcaaaggatt actaaaagaa gaataaccca tgtctaaaaa gcaggagctg tgacaaaagt
    12241 tgcagctcct tgtttgaatg attgttttta acatagctgt gtttttgatg ccttcatttt
    12301 tatacttatt tgtaagcggt tctcaaacaa tttcgttctt tttcttagac tttttgatta
    12361 taattagatt catatgaatt ggaggtcact taatgatgaa ttttaagcaa ttattattgc
    12421 atgtcaatgc gcgccctttt atcgaccaag ctcgatttgg tatagaacgg gaaggacagc
    12481 gggttgatct tgcaggaaat ctagcaaaaa ccgatcatcc agcaatcttt ggcgatcgat
    12541 cctatcaccc ctatatccaa acagatttta gcgaaacaca aacagagatg atcacccctg
    12601 ttaccgattc tattcccgaa ttatttcagt atctcgctgc tgtttatgat gtgactgctc
    12661 gttctatacc gaaagaagag atgatctggc cattaagtat gccacctgcc ttaccggaaa
    12721 aagacgaaga gatcattatt gcaaaattaa aaaatttcga agatgtcttg tatcgacgtt
    12781 atttagcaaa agaatacggg aagcgaaaac agatggtaag cggtattcat ttcaattttg
    12841 aatttggcga tgagttgcta agaacattgt tcagccatca ggaagaattt caagattttt
    12901 ccgaattcaa aacagaactt tatttgaaaa cagcgagaaa ctttatgcgc tatcgctgga
    12961 tgatcactta tttattcggt gcttcaccaa tgagtgagaa gaactacttt ttagatgaat
    13021 cacatccgca agaacctgtt cgcagtattc gcaacagtgc gctgggctat acgaatcatc
    13081 caaatgtgaa agtttcctat gcttctatga aacagtattt agcagatatc gagcgaatga
    13141 tcgaagaagg gaaactttcg gaggagaaag aattttatac accgcttcgt ttccgaggcg
    13201 gaaagaaagt cgcagattta gcaacaacag gtgttcgtta tattgaattg cggaatatcg
    13261 atttgaatcc ttatgcaaga ttgggtatca atccagagca agttcggttc ttacaattat
    13321 tcctgatgta catgttatgg acagaagaaa aagaagactg tgaccaatgg gtagcagaag
    13381 gtacgactcg aaataacaaa gtagcactag aacaaccatc agatcaaaca gaatttcatc
    13441 aagaaggtag agaaattctt gaaggaatga agcaaatgct ggttgaattg gattggttgg
    13501 attctcttta tttagtagag gaagcattga ctcaaatgga tcatcctgaa caaacgttag
    13561 cagcgaaact ctatcaagaa gcacagctat cgagtcagca agaagttgct gtggcattgg
    13621 gacatcaata ttataaagaa agccatgaac gtccctatca gttggccggt tttcgcgaaa
    13681 tggaactttc tactcagata tttatgttcg atgccatcca aaaaggtgtc caagtcaaag
    13741 tattagatga gtcggatcaa tttttgcgtc tgcaattcca agaccatgta gaatatgtga
    13801 aaaatgcaaa catgacaagt aaggacagct atatcgtccc acttatcatg gaaaacaaaa
    13861 cagtaacgaa aaaagtctta aaagaagctg ggttccgggt accaggaggc gcagaatttt
    13921 catccatgga agaagcggta aaagcttatc cgagatttgc cgagcaggca tttgtgatca
    13981 aacctaaatc aaccaattac gggttaggta tcacgatttt caaggaagga gctagtctgg
    14041 aagattatca agcaggttta gccattgctt ttcgtgaaga cagttcggtt ttagtagaag
    14101 aatttatgcc gggaacggaa tatcgtttct ttgtgatcga cggagaagtc caagccatca
    14161 tgttgcgagt ccctgctaat gttattggtg acagtatccg aacagtgaaa gaacttgttg
    14221 aagaaaaaaa tagtgaccct ttgcgtggaa cgaatcatcg tgcaccttta gaattgatcc
    14281 aattaggtga attagaacaa ttgatgttga aagaacaagg gttgacgatc gaatcagttc
    14341 cccaagccaa tcaaattgtt tatctaaggg agaactcaaa tatcagtaca ggtggtgatt
    14401 cgatcgatat gactgatgaa ttttctgaag cttacaaaaa aatcgccgtt tctgcggtag
    14461 aagcactagg agctaagatt agtggtatcg atttgattat accagataaa gaaatcgatc
    14521 ctacaactga taaaaaagca tatgggatca tcgaagcaaa tttcaatcca gccatgcata
    14581 tgcatgttta tccttttgca ggaaaagaca gaagattgac gatgaatgta ttgaaattgt
    14641 tatatcctga agtattttaa aagaaaaagc ttttgacaga ttgtcctatt tgatgtcaat
    14701 ctgatcaaaa gctttttttg catgtaaaaa tttatgtgtt ttttcgtttt aaaataaacg
    14761 atcccagaag ctttttttct tttttggttt ttctttttca gctggcgaag agttttcttc
    14821 tgggtatttt ttttcgatat caatcacagg ttcatttact gcttcttttg ctgttaagag
    14881 caatccgaat tcctcaggat ttgcagtgac gtaatcgttg actacagtga aacggctatt
    14941 gtttttcgtt gctagctgga tatattggtt ttggacagaa aggggtagag ccccattcaa
    15001 gagtatggaa gcatctggat gttttttgag ttcttttaaa aagttttctt tgttttttgg
    15061 ttggatcatt tcagcaacag tcatactaag atagcagcgt tccctgaatg tccctaaata
    15121 tttatgctgt tcttcagggt tcactaaagg tgtaccataa agtcccttat ccaaatggct
    15181 ttttacatca tcttgtgcca taaaaaatac cctccttctt ctctttgtct ttcttacgat
    15241 tatagcatat tcccaataat acctaaaaca tatgaaaaag cggttgcttt tgttcaacga
    15301 ttcacttaaa ataagaatga aaatcattct caattagaaa ggtggattgg atgcgctacg
    15361 gacgactagt agcattatta gttattttaa gtagtatttc attgttcatc ggagtatcga
    15421 atgtacaatt gtcagaactg ctccagttag atgggcaaag cttacaaatc ttgctagtga
    15481 ctcgtttgcc acgaacgatt tgtttgatcc ttgtgggagc gaccagtagt atttgtgggt
    15541 tgatcatgca acatttgacc caaaataaat ttgtttcacc aacgactgcg ggcacgatgg
    15601 atagtgcgcg tttaggtatt ttagtggcta tgattttcct acctggtgca tccttgattg
    15661 tccgatcctt gacagcattt tgctttgctt ttgcaggaac attgcttttt ttatccctta
    15721 cgcgattgtt cctccagaaa aatcaattga tgcttccgtt gattggagtc atgttaggga
    15781 atatcattgg ttcagttgct actttttttg cttatcaatt ccagttggta caaaatatga
    15841 cttcatggct tcaagggaat tttgcgactg tgatacgagg aaattatgag ttattgtatg
    15901 ctgttgttcc attgttgctc ttggtttggc tatttgctta ccgatttacg atcattggtc
    15961 tgggcgaatc gtttgcagta aatctgggaa tagattacca gaagatgcag tttttaggca
    16021 ttggcttagt atcattggct agtgcagtca tgttgatcat ggtaggaagc attcctttta
    16081 tcggagtgat cattccgaat cttgtctcta gaatatatgg agatcaagtg cacaaaacaa
    16141 ttggcgtaac tgctttagca ggtagtttgt tcttagtatt ttgcgatctt gtcgctcgcg
    16201 tgttgatttt tccttatgaa ataccagtca gtgtgatcgt tggaatcatc ggtggggcta
    16261 tcttccttta tcttctgata aggggacgac gatatgctta aaaaaaagcc atctattttt
    16321 attggtatgc ttgttcttgt tttatttgtt acaggaatgt acttgacgat ccatacatat
    16381 ggaaactggc attttgcatg tgcattgagg agtaagaaaa tttttgcatt tatccttgtc
    16441 tcgctagctg tcagcagtgc cacaatcagc tttcagacaa ttacgcataa ccagtttttg
    16501 actccttcag tattgggact tgagtcgtta tatgtccttg tccaaacggt ccttttcttc
    16561 tttatcggag gagtgacgat gcttcagcag acaggtacag tttttttctt attgaacgtt
    16621 gggctgatgg tcgggctgag tctattgtta tttttctttc ttttgaaaaa aatgagagcg
    16681 aatctttttt tattactgat gtcagggatg attttaggta cgctttttgg tagtgtcagt
    16741 acctttttac aagtcatcat ggatccgaat gaatatgatt tgcttcaagg cagattattt
    16801 gcaagctttg gcaatatcca gagtcagtat cttttgcctg cttctttgat cattatcggc
    16861 tgctggtacc tgttatttcg atatcgacat gtgctagacg ttttccatct cggaaatgaa
    16921 caagcagtaa acctcgggat agacacagat cgttttgaat ttttgatttt atgttgtgtc
    16981 agtcttttga cagggacatc aacggctctt gtgggtccag ttactttttt agggtttatt
    17041 gtcgcaaata tcagttatcg tgtcttttct acttatcgtc atggtcagct atttatcgga
    17101 agctttctgg tagctatgct gtttctgaca ggaggacagc tcgcagtaga gcagattttc
    17161 ggctggaata caacagtgag tgtcgtcgtt gaatttgtcg gaggaattta ttttgtcaca
    17221 aaattgattc gcgaaaggaa gagtatccta tgattgattt acaaaaggtc aataaactgt
    17281 accatggaaa atcagtggtt aaagatgtac agttaacgat tcctgaaaag aagttgactg
    17341 cttttatcgg tccaaatggt gctggaaagt caacggtgtt gtcgatgatc agtcgtttga
    17401 taccaaaaga tacaggtgaa atctatttag accataacga agtaaaggct tggaaatcca
    17461 atgaattagc caaaaaatta tcgatcctac gacaatcgaa tgaaataaat gtccaaatca
    17521 ccgtgaaaga attagtagaa tttggacgat ttccttatac gaaaggaaaa ttgacgacaa
    17581 aagacgagga aatcgttcag gaatcactgg aacaccttgg gttagaagaa atagcggatc
    17641 aatcgattga cactctttca ggcggtcaac tccaacgtgc gtatattgca atggtgctag
    17701 cgcaagacac ggattatatt ttactggatg aacctttgaa caatctagat atgaattttg
    17761 cggttcaaat catgcagatt ttaaaaaatt tagtacaaga aatcgggaaa acaattgtta
    17821 ttgttcttca tgatatcaac tttgctgcaa gttatgcaga cgaaattgtt gcaatgaaag
    17881 atggacagtt atttacgcaa ggaacgaccc aagaaatgat aaaaaaagat gtattggatg
    17941 aattatacga aatggacata cggatttgtg agattgaagg ccgcagattc tgtctttatt
    18001 tctaaaacta acattaggag agaagagatg aaaaaattag tagtaggtat cttgattcta
    18061 gcatcatttg gtttagcagc atgtggaaat agcaacaaca ccagtcaggc agacacaaaa
    18121 aaaagcagca cacaaactga aactacattg acgataacag atagtaatgg agaccaaatc
    18181 gaggtgccaa ataatccaga aaaagtcgtc gtattcgaca atggttcttt agatacgatg
    18241 gatgctcttg gtgttggaga taaggtagtt ggagcagcta caagcagcct tcctgagtat
    18301 ctttcatcct ataaaaaagt agaatcagca ggtggaataa aagaaccaga cttagaaaaa
    18361 attaatcagc ttcagccaga tctaatcatc atttctggcc gacaaagaga ttttcaaaaa
    18421 gatctaagtc aaattgctcc gaccattttc ctttctttag atgcgaaaaa tccatgggaa
    18481 tctttccagc aaaatgtcac tgctttaggg gagatctttg gtaagcaaga agaagctaaa
    18541 actcaattag aggaattatc tagtgcaatc gatcagacga aaaagaaagc agaagctact
    18601 gacaaaaaag cactagtcac acttgtgaat gaagggcaat tatcagccta cggatctggt
    18661 tctagattcg gtttcatcca tgatttattt ggctttgaac aagcagatga tcaaatcgaa
    18721 gcatccactc acggtcaaag tgtttcttat gaatatgtgc tggaaaaaaa tccggatatc
    18781 ttatttgtag tagacagaac aaaagctatt ggtggagacg acagcaaaga tgatatttct
    18841 gccaatgaac tggtagcaca aacgaatgct ggtaaaaatc agcagatcat ctcccttgaa
    18901 ccagatgtct ggtatttaag cgggggcggt ttagaatcaa tgaaactgat gatcgaagat
    18961 gtgaatcaag catttaaata gcataaataa cagaggatta aactctcata ctatgtaaca
    19021 ataatgtttc ttacattctt aaatagcaag agcctatcct caaagaagtt atcaaaaacc
    19081 atccgtaaaa tcgtattgat gattttacgg atggttttta tttatttgag aaatcttttt
    19141 tcaacgtgaa ttcattatct acatcatacg ggttgattct agaatgggga gtggcaaaaa
    19201 atagtgtgat ccattttttc agcatcaaga tcgcctcctt tgatttgaat caaaggtaca
    19261 aagcgaaact tagataaaac ttaaatttag atatttttat tttgtaccga tctcttcttt
    19321 aaacgatgtt ccatctgctg cttctcggca ataagtccac tagactagaa aatatgagaa
    19381 gctattttct gtccagcgtt cttattactc aaaacaaatc gcagttgtca caatctcttc
    19441 taaacgatgt tccatctgct gcttctcggc aataagccca ctagactaga aaatatgaga
    19501 agctattttc tgtccagcgt tcttattact caaagcaaat cgcagttgtc acaatctctt
    19561 ctaaacaatg ttcaatcagc tgaccttcgg tattagctca aagcactgat ttcacaacct
    19621 ctatttaggc atgtgtctca gcaagcggtt gatagttgat gatctgcatt tttcgataaa
    19681 aatcggcggg aagcttactc caccgtttta gtaattgata gaaataactc tcttctgctt
    19741 tgattttata ctcgcttaat atcaatccaa aactattcgt tttttttgaa gctaattttt
    19801 tataaatatc caatgctttg gttttatctt cattgacaaa gagtgttttt acttcaatat
    19861 aagaaccctc gatattttgt gtttcttcga taaaatagat gttttttata tattccttca
    19921 tagtgattcc tccaaagaag tcttttattt gataagttta ctctaccgaa gagttgttaa
    19981 taatccgtga acaaaaagta attctttttc caatagttgt taaaggtttt ttatttttta
    20041 tagtattttt tgttatttaa gaaaggaata aggttaaaca aataacaggt tgctttttgg
    20101 gtctttttcc gttcaagaaa ctgaacgaag aaaagggaaa gttgtctctt ttgtgataag
    20161 agaaatcata ataaacgaat gacttacttt ccaataaatt gagaacagtc agaaccagac
    20221 aaattagttg catctgatga ataaagtgat tattctatga catgaggtga tacagatgaa
    20281 attagaagtg acaaaagaag cacaagaagt attgaagaat aaattagaag cagacgatca
    20341 acttttatta gatatcgaaa acggtgacgg tccatttgca gaaagccaag tcagttgcca
    20401 attggatacg gcatttcgat tgatcattgt caaaaaagat acacaaacag atctaagtct
    20461 ttattctgta gtggctgata caccagttgg accaatcaaa atcaaagaca gtgcaatact
    20521 ttacatggat gatccatcca cacttgcttt agaaccaact tataatagtt tacaattaaa
    20581 aggaccaagc ggcttgagaa aaggcaactt gcaagttgtt cgaaaagact agagggataa
    20641 aatgggaaaa ttggcgatca tcagcgacct acatgtggac atcaataaat tcggcgaatc
    20701 agagctgatg cagctttggc aagttttgca aaagcacaag atcactcggc ttcatctagc
    20761 cggagatacg gctaataaag tagaccgttg cctagctgtt gtcgaatttt tcgtcaataa
    20821 aggattatct acgacatttc atttcggcaa tcacgagttg gcagatgtgc ccaatgaaca
    20881 gatgatggac cattttccag acccacattt tttaaatgaa cggtatattg ctttaaatga
    20941 gcagaccgtt cttttaggga tgaatggctg gtacgactat cagttttccg aattgaaaga
    21001 ttacgaggca atcgtacgaa tgaagcgttt gtattggtat gatcggttca tccagcgtgg
    21061 gaaaacagat ctcgaggtca atgcagaaat gcttgcagct gcaaaaaatt tgctagacca
    21121 gctggaagca aaaaaacttg aggtgatttt atccactcat ttcgttccta aacgggcatt
    21181 tatcatgtat caatctgctc cttatgagcg ttggaacaaa ttaaatgcat ttttaggatc
    21241 agaatcattc ggagaattac ttgaccgtta ttcaaatatc aaacaagtag tttttggcca
    21301 tacgcatcgt cgctttgaag ataaatccat cggtcacacg atttacagct gcagaccttt
    21361 tggttattat tatgaatggc agctgacccg agattttgtc ttgaaagaac agttgctaga
    21421 cagctatgat cctatgaaat tacgtgtcct cttgcgtgcc catcacccag cttttcaggc
    21481 ctatcaaaca caacatttgc aagaggaatt tgaacaagcg atgacaatcg ttccgtattg
    21541 agacagcatt agtaatgtgt aaaagaaaat accttgtaaa acataaggat aagtaagagc
    21601 agtagatcca tttatcgtgg atatgctgct ttttttgcgg aaaataatca gcgaatgttt
    21661 atttgatcgg atgatttgta actcgcagag tactagatgc aagcgtgtat tctttttttg
    21721 aacgataatc acaaaaaaaa atataatata cattttttat ttaattataa ttaatattga
    21781 ttgaagtcta agcagtcaag tcacattttg atagatagga gggggactta cgaattattt
    21841 taaaggctat gacagctgta tttctagatt gtgtttgcat caagttgcaa taaaaactag
    21901 aaatcaaatc gggctgataa tcatttataa ctaatcgata cataggacca aatataggtc
    21961 tatgttcttt tatttcataa gtatcgtaaa acagaatttt gctatggatt aatccaatcg
    22021 actgatatgt ttagtaaata aagaaagagg aatgtcaatg atgaaaaaag aaatcgtatt
    22081 aacagcagca atgatgttat tcagcatggt agcaagtacg acttttgttt ctgcaactga
    22141 agtataccca aaggaatata acacagaagg aacgattaca tttgaagcag gagatgaggg
    22201 tgtaactcct cctgttgatc cagagaatcc tgatcccaat aaacctgtcg atccatcaga
    22261 tcctccaagc cctggaacag gaggggcatt atcgattgat tatggatcaa aattcaaatt
    22321 tgggactcaa aaaatttcta ctgcggataa aacgtattat gcagctgctg atgtgatgaa
    22381 cgatgggtct agaaaaccga catatgtcca agtgacagat aggagaagta ccttatctgg
    22441 gtggaaatta agtgtctctc aaccagaaca gtttaaaact gccagtgggg atgaactagt
    22501 tggagcacag ctgaaattta cgaaagggca ggctgtttct ttggttgatc caacatatac
    22561 gccgcaaacg gtgaattctg aattaacgtt gacaccggga ggaaacaata cgttagcaat
    22621 taatgctaaa tcaggaacag gtgtggggac ttgggtctat cgatttggtg caaatgaaaa
    22681 tgaaaatcaa gatgcggtac agctttcggt acccggtaaa tctgtcaagt tagcgcaaca
    22741 atattccact aaattagttt ggacattaga agatacacct aacaactagt ttactgaaaa
    22801 gaactgttaa aaataaatac gtagtcatat tgtcttgcga ggtaatcgag caaaaattgt
    22861 caaaggctag gctaaatttg tttggatacg aatagttcat ctaggaattt aacgatcagg
    22921 aggaaaagat atattgaaaa agaggaggat cgtgctcatt ttaagcagtc tcttagtgac
    22981 taatatcttt ccgatcatag ggtactcttt ggaattgaat cagttggagc atggaaatag
    23041 aagagacgaa gaaagcaaac tcacttcgac attcgacagt ttaatagaat ccaaccagac
    23101 acaagaaagc agtgaggatt taagtatttc gagtagtgga gagaccactg acaacgtaga
    23161 agctgagggt tcggacaaac aaggactaga agatgcacca atttctgcgg ttgagctgcc
    23221 tgacgatccc aatattattt ctatcgataa aatttttcaa acacctatcg gtactagcac
    23281 gtccatattg gaaaatggga agttactgca gttgaaccca gcagaaaggt ctcaaagagg
    23341 agctatttgg tcaaataaga agattagttt actctcggat tttacattta aatcttattt
    23401 atacttagga aatcaacgag atagagcggg agatggcatg acctttacat tgactggcga
    23461 tcctagaatg gctagtaatc ccagtcaagt tattggatcg ccaggtatgg gaattggctc
    23521 atattctact cgttctggcc aaccttacat tgcgaatgcg ctctcgattg agtttgatac
    23581 gtataaaaat agtggttctt ctgatcgaat ggatcgagag ataactgctg ataatagagg
    23641 gcatggacat gtcgcttttg tcacgcctaa accaaataat aataattatt ctggagagca
    23701 tactggcgtg acggtagctc ctacatttct ttctaatggc acatggcgct tgttaataat
    23761 taaatgggac gcggagcaaa gaagtcttac ttatgatttg gaaggtgtcg gtcgctcttc
    23821 tcggattata cctgatctca ttaggcagtt tggttctaca gaagtaaatt gggggtttac
    23881 ttcttcaaca ggtacttttt ttcaagaaaa tgctgtcgct gtcacgcgac ttccatcgaa
    23941 tgtcacatca acagctgatg taaaagtcaa cgatagtgat tatggggcca tcgctgaagc
    24001 tggaggaaac gataagataa ctatcagaaa tacattgaag gtcgaggggg attttcttga
    24061 atataagaaa atgcagacta gtatcgacct cccacaagaa ttaactattc ccgagcatat
    24121 cttgatcaat ggaaatcaaa ttgatgccaa agatatcttg ataaccggaa atcgagtcac
    24181 gctaaatctg acagattatt ttattgctgg agatacgtta ataatcgaat tggagacaac
    24241 tttgcaggaa gtaattcctg aaaaagtact cactatgcat tttgaatacc ttgaaaacgg
    24301 gaatttgatt gagcaatcga atgaaataaa cctaaagatc gcaaagctga aggagataga
    24361 tatccatgtt tattataaag ataaagacag tcagattgat attgctgaaa tgaaaacaat
    24421 cactgggaaa ataggagaga agtataaaga aactcctatt gatattgaag ggtatgtttt
    24481 tgactcggat tcagcgaata cagaaggcat cttctccgaa aatacagagg atatctattt
    24541 ttattacaga aaaggcaaac tttatctctc aactgctcct caatatgtca attttggaaa
    24601 acataaaata tccagtagac cattaagtga attcggacag ttttcaggtg ggttgaaaat
    24661 tatagatgaa cgggcttttg gaagttggaa attacaattg aaacaagaga aagttcttac
    24721 aaatggagat tttgagcttc cagaagctct ttcctttgtt acctctgaaa atacaacggt
    24781 tatagggaca tccgctgtta cgattttcga aagtgatcaa aagggtgaaa gcgatttgtc
    24841 ctctctttta gattctacag ctcatcgtgg aatcagaata aatattcctg tagaaaacca
    24901 aagaatcgga acctttgagg ggaaactatc ttggatactt gcagatgtcc caggaaatta
    24961 acaggaagag aagaatataa caaaattatt gagtttcaag aagggcacag gtaccatagg
    25021 ctcttgtgtc ccacagagac aagattttgt tatagtcttc tcttacatat gattaaagga
    25081 gcgattttct tgagaccaaa acaacaatta tttataacac tcctagttat tttaggggca
    25141 ctttttaaca atatatcagt gtcagctagt gcatttaatt tttcagtgac gccgataact
    25201 tcagagaatc aaatcgacaa gagaaaaact tacttcgatc ttcaacttga tccggatcag
    25261 gaagtagaag taaaagcgga attgagaaat gatacagaaa aagaagtgaa aatcgatatt
    25321 tctgtgaata gcgcaaccac caattcaaat gtgatggttg aatacggaaa aaacgagatt
    25381 gaaaaagaca tgagcctgat ctttgatctg gtagattatg tgagctatcc acagatagtg
    25441 acgctaaaac ctaaaagtgt ccaaaccgtt gcttttcatg tcaggatgcc gaatgagcga
    25501 tttgatggtg tattggctgg tgggatcaca ttccaagaaa tccagacgga aaaggaccaa
    25561 acagaaacta aggatcaaag cttatcgatt gagaatgaat acacctatat tattgctttg
    25621 ttgatgcaac agacactgaa cgaagttgct ccgaacctta ctcttcatga agttaaacca
    25681 gatcaaatca atgcaagaaa tgtcatctta gcaaacgttc aaaatgatca aaaaacctat
    25741 atcaatcaag tagttattga aacaaaaatt acaaaaaaag gacattctga agttttatat
    25801 caagaggaaa aagaaggatt gcagattgcg ccgaatacaa atttttcttt tccaactgct
    25861 ttaaatggtc aaccgctaac cccaggtgaa tatcacctta caatgactgt tttaggaaat
    25921 gaaaatgaaa atggaaaatt ttcgagaaaa aaaggaaata ctacgatcaa ctataccaat
    25981 caatgggtat ttgaaaaaga ttttacaata gacggaaaag tagccaagga attgaacacg
    26041 acggatgtta cgattaaaaa agacaataat tggatttata tcttagtagc ttcacttttc
    26101 ttattgttta ttttattatg catacttttg tttgttttga gaaaaaaaga agagaaagaa
    26161 aagtaaggtt aagataacaa atagtaaatt tttattattg taggatggtt gggctctttt
    26221 tataacaata taaaatatac cattttatta ataaaaatgc atactagtaa atgatataat
    26281 tctaataaca ggtttgatcg cgagaaaggc caaataaaac gctctccaaa atgttaaaaa
    26341 aaagacagga cgatgtatat tatacaaaca agaatcgctg acaacagtga ctcggatcca
    26401 ggaaatcagt tttatcccca gctagttggt gaatgcatgg aaagtaatat acgtagaaag
    26461 agctgatgac taatgaatta tgagtttttt ttaagcaaag aagatcacag aagttattca
    26521 ttacttaaat atttagaagc aagctcaaat ttatcagagt caatatcgaa tgttcaagaa
    26581 gagttgtcgc tatcgacatt tctcttgaaa aaaacgatcg ataagctgaa acatgattta
    26641 caaaagttcg atctagagaa aaatttgcga gtaaccgtca gtgaattgga tatatgtctg
    26701 gaaattaatg gaaaatgttc tagcaaagct ctgttatctc gttatataac ggattcttta
    26761 tctatgaaaa tggtattggc tttctttcag gaaaaatatg tctcagtaga aaattttgct
    26821 aaacagcatc acgtgagtta ttctgtggct tataaagtat tacaaagact aaaaagaaat
    26881 ctaaaaaaat atcaaatttt ttttgaaaag agaaagtt
//
LOCUS       DAMYXM010000030        26574 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.031, whole genome shotgun sequence.
ACCESSION   DAMYXM010000030 DAMYXM010000000
VERSION     DAMYXM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.031"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(324..893)
                     /locus_tag="PNS21_001502"
     CDS             complement(324..893)
                     /locus_tag="PNS21_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDI5159215.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="PNS21_001503"
     CDS             complement(967..2256)
                     /locus_tag="PNS21_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159216.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2610..3458
                     /locus_tag="PNS21_001504"
     CDS             2610..3458
                     /locus_tag="PNS21_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDI5159217.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3472..4545
                     /locus_tag="PNS21_001505"
     CDS             3472..4545
                     /locus_tag="PNS21_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HDI5159218.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="PNS21_001506"
     CDS             4535..6706
                     /locus_tag="PNS21_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDI5159219.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8974)
                     /gene="copB"
                     /locus_tag="PNS21_001507"
     CDS             complement(6818..8974)
                     /gene="copB"
                     /locus_tag="PNS21_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HDI5159220.1"
                     /translation="MHMGHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHMGHNHG
                     DIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGMEFPFQ
                     VTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYIYSVYS
                     FIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPESVKRL
                     TKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGIKKQAN
                     DKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAKWLFYV
                     ALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAAKNGLL
                     LKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGALEANAN
                     HPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLGLNVEQ
                     ALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVMLTGDNK
                     NAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLARASIGM
                     AIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILAIPLAA
                     GILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9117..11303)
                     /locus_tag="PNS21_001508"
     CDS             complement(9117..11303)
                     /locus_tag="PNS21_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDI5159221.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11303..11512)
                     /gene="copZ"
                     /locus_tag="PNS21_001509"
     CDS             complement(11303..11512)
                     /gene="copZ"
                     /locus_tag="PNS21_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDI5159222.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11525..11965)
                     /locus_tag="PNS21_001510"
     CDS             complement(11525..11965)
                     /locus_tag="PNS21_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HDI5159223.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12039..12512)
                     /locus_tag="PNS21_001511"
     CDS             complement(12039..12512)
                     /locus_tag="PNS21_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HDI5159224.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            complement(12822..14288)
                     /locus_tag="PNS21_001512"
     CDS             complement(12822..14288)
                     /locus_tag="PNS21_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI5159225.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(14599..16683)
                     /gene="cadA"
                     /locus_tag="PNS21_001513"
     CDS             complement(14599..16683)
                     /gene="cadA"
                     /locus_tag="PNS21_001513"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDI5159226.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            complement(17207..17566)
                     /locus_tag="PNS21_001514"
     CDS             complement(17207..17566)
                     /locus_tag="PNS21_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDI5159227.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(17596..17937)
                     /locus_tag="PNS21_001515"
     CDS             complement(17596..17937)
                     /locus_tag="PNS21_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159228.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(17934..18608)
                     /locus_tag="PNS21_001516"
     CDS             complement(17934..18608)
                     /locus_tag="PNS21_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDI5159229.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            complement(19404..20702)
                     /gene="asnS"
                     /locus_tag="PNS21_001517"
     CDS             complement(19404..20702)
                     /gene="asnS"
                     /locus_tag="PNS21_001517"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDI5159230.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(20742..21932)
                     /locus_tag="PNS21_001518"
     CDS             complement(20742..21932)
                     /locus_tag="PNS21_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDI5159231.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(21953..22480)
                     /locus_tag="PNS21_001519"
     CDS             complement(21953..22480)
                     /locus_tag="PNS21_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HDI5159232.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(22527..25316)
                     /locus_tag="PNS21_001520"
     CDS             complement(22527..25316)
                     /locus_tag="PNS21_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDI5159233.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(25463..25663)
                     /locus_tag="PNS21_001521"
     CDS             complement(25463..25663)
                     /locus_tag="PNS21_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDI5159234.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(26115..26435)
                     /locus_tag="PNS21_001522"
     CDS             complement(26115..26435)
                     /locus_tag="PNS21_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159235.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
BASE COUNT         7472 a         5626 c         4665 g         8811 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgtcccatgt gcatggaatg gtccatctgg ctatggtcat
     9001 gtttcatctc catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt
     9061 tatgttcatg ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac
     9121 tttttcatgt gccgatttaa gctcagggag ttcaacaata cactgacgga actaaacgcc
     9181 atcgctcctc ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc
     9241 gtattataga caaaagccca gaaaagattt tgtttgattt ttctcaatgt caccctagac
     9301 aactcgattg tttgtgcaat ggatgccaaa ctgctactca tcagcgtcac atcggctgtt
     9361 tccatcgcga tatccgttcc acttcccatt gccataccaa catcagctaa tgctaaagcc
     9421 ggtgcatcat tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt
     9481 tctacgtagg ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata
     9541 ccaacttgtt taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca
     9601 tctaatcctt tattctggag ctgcttgatt gcttgtttgg cttctagttt gatttgatca
     9661 gccacggcaa tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc
     9721 tgctgttcta attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt
     9781 aatcgtttac gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca
     9841 cctgggtgag ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat
     9901 tccacgattg ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat
     9961 aggacttctt tagaaccgac tagatctgta actttgggct ttccttctgt aatcgtccca
    10021 gttttatcca aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta
    10081 attaaaattc catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct
    10141 aaacctaatg cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt
    10201 tgccaatcac cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg
    10261 atcggaacaa aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct
    10321 tgagcatctt ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgtg
    10381 acttccatgt gtagcagacc attcgtattg attgtcccgc caaaaagcgt gtcatccgga
    10441 ttcttttcta caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct
    10501 gaaataattc gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca
    10561 acgactactt cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt
    10621 tttgtttgaa gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt
    10681 tccaaatatt tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga
    10741 tcttgggggt gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt
    10801 ccaattgcta ccaatacatc catatttggt gctttcgact tgattgcatg gtacgcacct
    10861 ttataaaaac gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt
    10921 ggaaaatgaa aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac
    10981 aacatcaacg gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa
    11041 tacgcttgct tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca
    11101 tagccgatgt tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca
    11161 cttgcttttt cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc
    11221 tctttttcga tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc
    11281 attttggttt ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg
    11341 cttgacaaat cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt
    11401 ttttcaaatt tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc
    11461 ttgctgcaca atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt
    11521 cctcctattg atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat
    11581 ctactggttg cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag
    11641 tggcttcttc tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa
    11701 aaagattttc cgtcgcactg cgaactgttt ccatctccga gacagctgga tgataaatga
    11761 atttttttcc ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag
    11821 ttttgatcgt tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg
    11881 ctgtcgtctg tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactaatct
    11941 ttactggcgt tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt
    12001 aacttatgaa tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt
    12061 ttcaatttag ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt
    12121 cttgcattag ctacttggat cagctgctgt atttttggtt cattgtttcc ttcaaaatgc
    12181 cagataaaag ataaaaattc taagtatttc ctgtttagtt tctccgaaaa tgattcaggc
    12241 atatctggcc gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag
    12301 atggcgagat aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc
    12361 tctaacgtag aagtataatt tccttcaacg atccatgacg gattagatac cataaacgat
    12421 tgctgttctt gcaaaaacca cttttttgcc tgaatgcgga aagccggtga tttctttgat
    12481 cttccttgcg agggacgatt ttcccgaccc actttgtcca atcacgatga tcttcattct
    12541 tctccctcac ttctctttgg aatttggttc gatcatacca cttataacgc cgcaaaaaaa
    12601 caagggactg tgacaaaagt cgtgtcaaag tcccttgttt cgaataaatg gtgttcaatc
    12661 agctgacctt cagcattagc tcaaaaaacg gcaaaatatg aggagctatt tttgtttggc
    12721 tgttcttatt acttgatgca ggacagcttt ttcacaacct ctgattcacg gtgttccaga
    12781 agtagcttct gtcacaacct cttgtaacaa gtatttctat tttaaggctg aaccactgtt
    12841 tctggttcag taatttcttt gtttttgtct cgacgagcaa ttgctggcaa gatcatacct
    12901 aataagatca agacaattgg tgtgatgata ttcgtgaata attggaacca ccatgcttta
    12961 ggatcagcag tataatctac ttttggcacc attcctagga tacatgcaaa cgcagtgaag
    13021 gcaaagcacc aagcaccaaa gataaaaccg atttttggat ttttaacaaa tttatattca
    13081 gaattgaatt tcttataagc tttattcaac atcatatatg ccaagaacac ccataggtaa
    13141 cgcattggca tgacaactga gttcaagttc gtcaaccatt ttactaattc attcatatct
    13201 ttgataccaa agatcggcaa caaaataatg attcctacta gtatacctgt cagaagataa
    13261 ccattcacca atgtaccttt acgattacgt ttacgtagcc agtttggtac aaattctgga
    13321 tccgcatctg ctaacaagat ttttagtggt gcatcaattg aaaaggctaa tgcggagatt
    13381 tgtccaatca tgtttgtcaa tgcgtaaatg atcatcaatg agttaccgat accatagtaa
    13441 ttacctaaaa tttgaaaagc actgtatgcc ccatttgcca tcaagtcttt agggatatta
    13501 tcgctagcaa acagcatacc cattgcgact gagcctaata cagcacaaat ccctaccatt
    13561 gctgctaaga agatcatccc tttaggaaat tcctttgcag gatttcttgt agagttgaca
    13621 taaggggata ttttttccgc ccctccaact gcaaatacta gcatcgaaac ggttgtgaaa
    13681 taactgaaat caaatgttgg aatatatgtt ttcacttgat tcatatgtgg tgtggcaaat
    13741 tccatcgttg gattgatgaa cggtgccgtt acagctaaaa gaataaataa gatcgacatg
    13801 acgaacatcg ctgttccggc cagtccgcca atcactttca aagtagaaag tccttttgtt
    13861 gataaatata aaaaggcaag aaatataata agtgaaataa ctgctaccat ttgtactgac
    13921 atcgtattta cgatatttcc atttccttgg actgcccaac caccagcaat cagaattgct
    13981 tgaggctttt gtgccaaata aggaatatgg acgacccagt aagtccaagc ggcatagtat
    14041 gccagtcgtt tattagatgt cgtattttcg acccatgaac tgacgcctcc tttactatcc
    14101 ttgaaggctg atcctaattg tccaacgatc aatgcgtatg gaataaaata aatggccaaa
    14161 atcaatagcc aagaggtgac aactgatact ccttgctgag catagttatt aactacattc
    14221 cccatgcccc aaaccatgtt aaatgcaatt aacgcaacag taaaccagcg taattgtttg
    14281 ttgttcattc gttctctcct ttatgataaa aatttgttat ccagatgtaa tcatattcaa
    14341 tttttaacaa acttacaaga cagtgttatt ttatgtgtgt attcttttat ttaatacaca
    14401 tcgttttttc atattttttc catcaaaaaa ataagttatt ttcttttatt tcattgacct
    14461 tattgaattt caagctattt tgaattttct gcaaaattaa aaacattagt tattttataa
    14521 tcctatctat tttctttaca tttttcatta agaaaaggat ataaaaaaaa gaggacgttc
    14581 attcgtcctc ttcattgatc aacgtttatt ttttacatgc aagcttcgca tggcattcaa
    14641 aacagctaat actgtcaccc caacatctgc aaatactgca tctcccattg atgcaaaacc
    14701 aaaagcacct aaggaaagta ccaaaatttt tatcccaata gcaaagacaa tgttctgttt
    14761 gacgattttc aatgttttct ttgctaaatg gattgcttct gcaatcttcg atggttgatc
    14821 attcatgatg actacatctg ctgcttcaat tgcagcatcg cttcccaatc cacccattgc
    14881 gattcctaca tctgctcttg ctagcaccgg tgcatcgttg attccatctc cgacaaatgc
    14941 ggtcttttta ttgttacttt gtaagatttc ctctaaccgc tgcactttat cttctggaag
    15001 caattcgcta tatatcttgt ccacgcctac ttgcttcccg atatgatcag caattttttt
    15061 cgagtcgccc gtcaacatga ctgtgttttt cacaccagcc tgttttaatt gaaccaatgc
    15121 gtctacggca tcttctttca aggtatctgc aataacaaga taaccactgt aactttgatc
    15181 gatcgctaga taaagcaatg taccgatttc ttgacttgaa ggaaaagaga tttcaaatct
    15241 ttccatcaat ttgtgattac ctactaatac ttgtttgcca tcaacttcag ctaagatccc
    15301 ttcacctgca aattcctgga tattagctgt tggtaaaagc ggacctgcgt agccttctaa
    15361 aacagattga gcaatcggat gtgtagaaaa ctgttctgca cttgcaacat attgcataaa
    15421 tttctttgga tcaattcctg ttggatcgat cgtctggatc gaaaagtcac ctttcgttaa
    15481 tgtgccagtt ttatcgaata ccactgtctc agtttgtgca agcaactcca agtaatttga
    15541 accttttact aatacaccga ttttacttgc tcctccgata ccgccaaaga aactaagtgg
    15601 cacggaaatc actaatgcac atggacatga gatcactaaa aatgtcaatg ctcgatagat
    15661 ccattcagaa aacgccgctc tagaaacaac aagtggaggt aaaacagcta ataaaacggc
    15721 caatcctaca acgactggtg tgtaatagcg cgcgaattta gtaatgaaat tttcagctgg
    15781 tgctttttta ctgctggcat tttctactaa ttctaataat ttgctgatag tagaattttc
    15841 ataagatttt tctacttgaa ctaaaaatgg tttattttta ttgataaatc cgcttaatac
    15901 tgtttcacca acatatacag atttagggac tgattctcct gtaagtgctg acgtatcgac
    15961 taatgattct ccttcgatga tcgttccatc taacggtaca cgttcaccag gcttcacaag
    16021 tacaaactga ccgatcagca cgtcttcagg gttcacggtc atcaatcctt ccgccgtctg
    16081 cacgtgagct gtttctggac ggatcgccat caattcgcga atcgattttc ttgaatgact
    16141 gactgccaat ccttggaaat actcgccgat ttgataaaat agcatcactg ctacggcttc
    16201 tggatattca ccgatcaaca tagcgccaat cgtagcaaca gacataagaa aattctcatc
    16261 aaagattttt cctttgacga tattcatcac tgccttcttc actacatcat atcctatcag
    16321 cagataaatc aataaatacc ctatcaagcg aattggttca ttaaaatcaa ataatagcaa
    16381 taaagcgata cctgcaaggg aaaataaaat ccggaacagc gtatttctct ttttttcatt
    16441 tccatgttca gatacttttt ccttatctat ttctgttact ttgacatctg gttctaattc
    16501 cttcactact ttttttgttt cttgttcaat cgtagccaaa tgatcttcct ctgcgtcaat
    16561 cgttagtttt ccagaagtga agtttaccgt tgcttccttg actccattga ttttctgaac
    16621 tcctttttcg attttcattg cacagttcgc acaatctaaa ccttctaatc tgtaactttt
    16681 cattttaatg acctcctcgc ctcaacatat gaatatctct tcataactaa ttataaatga
    16741 atggttattc atatgtcaag gtatccgaaa gaaaaaatga aaaacatctg catcggaaaa
    16801 tttcttccat acagatgttt tggcttattc tttctttttc tcttcgatca ttgccactgc
    16861 tcttatccaa cccgcacttg ccttcttgat cttgacagga tagcaactaa gtttgaagcc
    16921 tgttgctggc aatttgtcta aattcattaa tttttcgata ggacaatatc ttttttcaat
    16981 ccctgcaaaa tgtccttccc agatatacga agctccacta cgctttcaaa aatatagaaa
    17041 gagttcttta atgaaaatta tttcaacttt ccataaacag taaccccata taaataaaag
    17101 acgtaaaagt cacatagaag aacttataca ggaaaacgca tagagtcagg tgaaatacat
    17161 gtatcccacc tggctctatg ctttatctaa cctttattat tctcttttat acaatttctg
    17221 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
    17281 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
    17341 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
    17401 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
    17461 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
    17521 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
    17581 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
    17641 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
    17701 tgtgtgttgg attagttttc ctgatggtcg ctgatccaat cgttcttctg actgttgcac
    17761 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
    17821 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
    17881 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccttattt ccatcatttt
    17941 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcgac aattgcatct
    18001 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
    18061 gtatatttcg aaaaaccata agcaatccct aaagcattga atataccatc taaaggaacc
    18121 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
    18181 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
    18241 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
    18301 tgagcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
    18361 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
    18421 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaattccatc
    18481 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
    18541 gttgcttctg ctcggtatct tcctgcgtct gtaatgctat caccgataaa taatatacgt
    18601 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
    18661 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
    18721 tccttcaaac aattgaatga acgctcatca agtgtctagt tttttatgct atttttttct
    18781 taacgtgccg gattcccctt gattccttcg ttgtttggtc cagagagatc gatcacaccc
    18841 aagcttccgt caatcagacc actgatgacg ccatcgaaac atatatttat aggctcatct
    18901 aagacttcta gtaaatgtcc ctttactaat atctcttcta gattgcttgg gatagattgg
    18961 tcataacttt atctggtccg atgatttcaa gggtgaattg aacttacata tggattattg
    19021 catgctgaaa atacggacct actccatctt cgaatgttac tattagtttg ttttcaggtg
    19081 ctagatgctg ctttatttca acagcagaag catcatcaaa tcagatttcc atttgaatta
    19141 ccttctttgt aaatgtttga ttgattgcga tttcgttata gaaccttgtg aatcaaatag
    19201 caatttcttt tgctttcttc tcttgtctgc tggaaacttc cattatcccg tatttaaaaa
    19261 aaaagaactg cgagagttcg cagttctttt ttttaaataa acgttgctct ccggccacaa
    19321 gtcacaatag tcatcaaaca tgaaaaactg ttttttgaaa ttactccttg tgactcggag
    19381 ctgaacggca ggcgcacaac ctcttatgga taaattcggt tcaacaaacg tgggaatgga
    19441 atagcttcac gtacatggtc gataccagca atccaagtga ctgttcgttc aagacctaga
    19501 ccaaaaccag agtgcggaac agaaccgtat ttacgtagat caagatacca tgaatattct
    19561 ttttcatcca aaccgtgatt acggatttgt tctagcaaga aatcatagtc cgttgcccgt
    19621 tcgcttccgc caataatttc accgtaacct tctggtgcga tcatatctgc acaaattacc
    19681 acgtcttctc ttgttggatg tggtttcatg tagaatggct tgatggcttt tggatagttt
    19741 aggataaaga caggctgttc gaatgaattc gcaatgaacg tttcatgagg tgaaccaaag
    19801 tcttcgcccc attcgatgtc atcaaatcca tttttcttca gtaattcgat tgcatcatcg
    19861 taagtaatac gagggaatgg caattttgta taagttttta agacttcttt gtctcgtcct
    19921 agtacatcta atgcgtattc gcaatgatct aaaacatttt gtactaagaa tgctacatat
    19981 tgttcctgta cttctaaact ttcttcttga tgagtgaaag ccatttctgg ttcgatcatc
    20041 cagaattcga tcaaatgacg tcgtgttttt gatttttcag cacggaaagt cggaccgaat
    20101 gaaaagactt ttccgaaagc catagcagca gcttccatgt ataattggcc actttgagat
    20161 aaataagctt tttgatcaaa gtaatttgtt tcgaatagat ccgtagtgcc ttctggtgca
    20221 gaaccagtca agatcggcgg atcaattttg acaaaatgat gttgattaaa gaattcatac
    20281 gttgcacgaa tgatttcatt tctgatctgc ataatcgcat gttgtcttga agaacgcagc
    20341 cataaatgac gatgatccat caagaagtct gttccgtgtt cttttggtgt gatcggataa
    20401 tcgtggctct cgcttaccac ttccacattt gtaattccga tttcataacc aaattttgaa
    20461 cgactgtctt cacggatttc tccagttact aacacggatg tttcttggtg tagattttta
    20521 gctgtttgga aaacttcttc cgacacttcg ctttttacaa ctacaccttg gaaaaaggct
    20581 gttccgtctc ttaattgtaa aaatgtgatt tttccgcttg atcgcttatt tgcgacccaa
    20641 gcgccgattg ttactgtttc tcccacatgg tttttcgaat cgatgatttg aatttgttcc
    20701 acgtatgact ctccttttat attccttgaa tttcttcagg attaattttg acttttactc
    20761 tcaataaatt gcttgattct gcgtaccgct tcttctaacg tagcgcgatc agtagcataa
    20821 ctcatgcgga cattttctgg tgccccaaag ccttcgccag tcacaagcgc tacccctgtt
    20881 tcttctaaaa gatcttctac ccattgtgtg acattttcat atttacacat gcgtagcgtt
    20941 tcttttacat taggaaaaag atagaaagct ccttgcggct tttcaagaga aacacctggc
    21001 agttctgcca caagcggata taacgtattc aggcgttctt caaaggcgtt ccgcatttct
    21061 tctactgtat cttgctcacc tttcaatgct tcaacagctg catattgact gactgctgtc
    21121 ggattactgg ttgactggga agcaacagca atcattccat tgatgatctc agaattcccc
    21181 accgcataac caatgcgcca gcctgtcatc gcatatgttt tagacacgcc attgatgatg
    21241 atggtctgat ttttgattgc ttctgaaatc gttgcaattg gagtaaactc attgccatta
    21301 taaactaaac gaccataaat atcatcagca actatcaaaa tatcattttt tactgcccat
    21361 tcaccgattg cctgaagttc attcttgcta taaatcattc cagtcggatt cgatggagaa
    21421 ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt ctagctgttc aacagtaact
    21481 ttccagctat tggattcttc ccctttcaca aagacaggaa gtccttctgc cagttttact
    21541 tgctcaccgt aactgaccca gtaaggcaca ggaatgatga cttcatcttg cgggtcaagg
    21601 atcgtttgga aaagggtata caaagcaaat ttcgctccgt ctgttacgac tgtttgtgaa
    21661 ggttcataga caagtccata gtttttcttc agataatcaa cgattgcctg tcgaagctct
    21721 gggattcctg ctgttggtgt ataatagcta gctttcccat tgcggattgc ttcgattgct
    21781 gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc ccacagtcaa actcagcaca
    21841 tctctgcctt ttgcttttaa tgccttggcc tttgccgcag ctgccaaagt gacagacggt
    21901 tctaatcgtt ttgttctttt tgataactcc atcccattca ttcctttacc ttttaaattc
    21961 ccttgattgt cttgacttca tctccagatt caaagccaat caggtaataa ttcagtttcc
    22021 catttgtttc ttttcctact acttcccaca caggctgatc atcatacatc cctaaagcag
    22081 ctttttcgat ttggatttca ggatgggccg cagcaattgt ctgttttgct tcgttttcag
    22141 tcagtccctt tgtttgatcc aatactttta ctttttcacc agatttagga atgataacgg
    22201 cgatttcttt tcctttatca tttgtacctg tcagtgaaaa gtatgtcttc tttcgagtaa
    22261 accaataaaa atgatcggct tcctcaagat ttgcatattt ctttgcgatt tctactgctt
    22321 ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta aaaaatcgag ctgatcacga
    22381 tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg attaatcgtt ttttcctttg
    22441 tctgtttttt cttcaagggt cgtcgctcct ttatcttcat tccttttcat tatagcagat
    22501 tttaattatg aaaaaagaca gctcctctaa cttccttctt catttttctt taagaattca
    22561 tggatgtcct gatagatttc ctccatagct agctctttga tcggtacttt tttcggtaat
    22621 gcatggatga tccgtgcacc atattttgca gtgactagtc ttcgatccaa aataagcaat
    22681 acgccacgat ctgtctccgt ccttatcaag cgacctaatg cttggcgtaa cttcaaagct
    22741 gctttaggta cggattcctg gtaaaaagga ttgatcccct ctgattctaa ataagcgttc
    22801 ctagctttga ccatcgggcg aagcggatta tcgaagggta aacgtgtgac tacaacgatc
    22861 tgcaaagcat ccccaggcaa atctacacct tcccaaaagc tgtcagcgcc aaacaatacg
    22921 cttttttctg acaaaatgaa gcgtttcaaa agcttctccc ggcttccgcc aaacccttga
    22981 gctagtattt ctcgtccttt ttctagcata gacagatgca ttttctgata gaccttctgc
    23041 aaaacatcat gagaagtgaa taacacaagt attggccgat cttcttgttc gatcagacct
    23101 gtaagtgctg tctctaaata atcgatatat gcttgattgc tgacttgatg gacggcaggt
    23161 gcctcttttg gcacatagat ccttgcctgt ttctcataat cataaggaga ggcaatcact
    23221 ttaagaggtg ctgcttcaat gccaagccgc ttagagagat agtgtcgatt agggccgatc
    23281 ttcagcgttc ctccaatata aagaatacga tcatatcgag gataccaaac agtcgacggc
    23341 aacatagccg cttgaaaatc atggagatga aaaatcatct gctgcgctgt gttcccagga
    23401 acgaaccaat ggatcaaatg atctcgccag tcttccagcc attgatccat cagcaagtac
    23461 tgcttgtcta attgatcaaa taagctatat aaacttgcta aatcgatccg catctggttt
    23521 gtccagcttt cttggataat ctcaaaagtc agacgcattt gtgactgtag ctgctggatc
    23581 tcctgataaa aaagctgcag gtgatcaatg gctgtcccag attccagtgg cagctgatcc
    23641 aacatctctt ttgtcatcat gacttcttct ggatactgtc cgttaggttg cttgccgctc
    23701 actaaactca ttaatccttc tgccatacaa atctgtgctt cgcatagcgc agaaagttct
    23761 tctcgataaa tcgataacca gcgtaaggct tcatgttctt tcggtaaata gctttgtatt
    23821 cggtcaaaca gctgatttgg ttcttctaat tgctggattt gtttatggaa atagttaata
    23881 cttaatcgtt tgtcatttac tttttcagcg atttccggca aatgatgtac ttcatcgatc
    23941 aacaaaaatg gactagctgg aagcaatggt tcttttcgct gtgtttcttg tattaaaaac
    24001 gcatgattga caatcaaaaa attactttgc ctgattttct tttgcaagaa acgaacaaag
    24061 tcttgttcgt aaaatgactg atttcttgct aaaaatccaa tacctctgtg cgcgatatct
    24121 gcaaagaatg aatggttcaa atttgttaaa tgtaattcat ccaaatcacc ggtcttcgtt
    24181 tgcgtcagcc aaactaagac cgccatttga tacaaagcat attgtttttg ttctggcggt
    24241 tccacaagtg tgcctttaaa acgctgtaaa tcaatatagt ggcgatagct tttgataatt
    24301 acaggttgta tgggctgatc tagcaattga ttcagcaatg gaatgtcttt ttctatgatc
    24361 tgctgctgca aaactagcga aacggtactg atgacagcgg gtttttcagg tgtagccaaa
    24421 taactcaatg gcagcagata accgattgtt ttacccattc ctgtagctgc ttcgatcatc
    24481 aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg cattcatcag tctgttctgt
    24541 tcttttcgat acatcagtgt tttaccaaac attttttctt ttgcttgttt ttttcttgga
    24601 tatgtccgct cgccataatg gacggatgtg aatagttcca cttctttttt ctgcaaagct
    24661 agcccatcga cgatttctaa tgagggatca agtggctctg gtttttcttt catttcttca
    24721 atcacatgat aaatgaaacg actcgtatcc atacctgtgt gtctacttaa tcgcgcaatc
    24781 tgctctaacg tgatgattgg caattttctc atccgttctt cgatcagcaa aagaagttga
    24841 cccgtcactt cggcatcact atctgcctga tgaggattct catgaataaa tccaaggctt
    24901 tctgacaaat cagctaagcg aaagcttggt tccgtcggca agaaaatttg tgcaagttct
    24961 actgtatcga tgcctggtat cttcaaacta ggaagtccgc atcgcatgag ttcatggttc
    25021 aaaaaattat aatcaaaata aatattatgc gcgacaaata tggtgtctgc caaaagattg
    25081 taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt ggacgcgttg attcgtgatc
    25141 cctgttaaat gctggatttg ttttgaaatc ttccgccctg gatgaatatc gatcgaaaaa
    25201 cgtgaagtga tccgacctga ttcaatcatg acgcagccaa attggatgat ccgatcttct
    25261 tttggattcg tcccagtcgt ttcgatatca acgactgcat aagttcttgg tttcaaaata
    25321 tcttcctctt ctcctgcctc taatgttctt ttgaaattat actacaaagt gcagaaaaat
    25381 actctcatcg ctttttaaag aaaatagaca aaaacgccca gacacgcaaa tgtctgggcg
    25441 tttatttttt agtataggtt aattaagctt tagtaacgtt tgttgcttga ggtccgcgtt
    25501 ggccttcttc aacatcgaaa gttactgctt ggccttcttc taaagttttg aatccgtcgc
    25561 cttggatagc tgagaaatga acgaatacat cgtttccgtt ttctgcagtg atgaatccaa
    25621 atcctttttc tgagttaaac cattttactg tacctgtttc cataataaaa atcctccttg
    25681 tgtatcaaca catattttgc aattacttga atggtgtata tctggaagat gtttatttga
    25741 aacaagctac cacagattac cgaacaaaaa ctactcctat agtatatcat accgtttaca
    25801 aaaaagctag agtttttcga ctttttttat taaaaaaagt tttctcagcg attacataaa
    25861 gtgaaaaacc gaactttttt taaaataaac gacatattgt tcagttggtg tttttttgct
    25921 ctgatgttct tctttaaact aaaaaaatta ttctttctga ctacttatcg gttctgtttc
    25981 aacggtcgag gctggggaag attctttagt aggtctgatg ttctcatggt atttctgata
    26041 gcctatgaca gttaccagaa gaacaaggca aaccaatacg atcaacgaga ttagtttttc
    26101 ttttttgctc atatctatac ctcataaata acaggatgat gtccatcata tttctgagta
    26161 tgagtaaaag caaaagctgc agctgtagaa tgtaagaaat gccagcaact ttcataatca
    26221 caggtcggac agtaccaaaa gtaaccaaat cttggctgca aagaccgttc ttctgtttca
    26281 ggaatcgcag aatcaagatc tgttgtctgt acataaacat ttttgtacag acgtgtgcct
    26341 gtaataggag accgctgtgt ttcttcagca aaaactggct gacttgttaa acttagggat
    26401 agaaacagca aacaccataa cagtcttttt ttcataaaga cctccttatc gataagtttt
    26461 tcccaattaa aatcatatct caagaacgcc cgttattcac attttgttca taaatgatgt
    26521 tttttgtatc ataatcgtca aaaagaggtc gtgaccacgt cacaacctct tttt
//
LOCUS       DAMYXM010000031        26440 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.032, whole genome shotgun sequence.
ACCESSION   DAMYXM010000031 DAMYXM010000000
VERSION     DAMYXM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.032"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(298..720)
                     /locus_tag="PNS21_001523"
     CDS             complement(298..720)
                     /locus_tag="PNS21_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDI5159236.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            complement(710..2884)
                     /locus_tag="PNS21_001524"
     CDS             complement(710..2884)
                     /locus_tag="PNS21_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HDI5159237.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2900..5242)
                     /locus_tag="PNS21_001525"
     CDS             complement(2900..5242)
                     /locus_tag="PNS21_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159238.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(5264..7807)
                     /locus_tag="PNS21_001526"
     CDS             complement(5264..7807)
                     /locus_tag="PNS21_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDI5159239.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7817..8215)
                     /locus_tag="PNS21_001527"
     CDS             complement(7817..8215)
                     /locus_tag="PNS21_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDI5159240.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(8296..8580)
                     /locus_tag="PNS21_001528"
     CDS             complement(8296..8580)
                     /locus_tag="PNS21_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159241.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8604..9602)
                     /locus_tag="PNS21_001529"
     CDS             complement(8604..9602)
                     /locus_tag="PNS21_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDI5159242.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
     gene            complement(9595..9942)
                     /locus_tag="PNS21_001530"
     CDS             complement(9595..9942)
                     /locus_tag="PNS21_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159243.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFLEGASVVVHEVVIVDKI
                     PKLLLERYGISHE"
     gene            complement(9958..10608)
                     /locus_tag="PNS21_001531"
     CDS             complement(9958..10608)
                     /locus_tag="PNS21_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159244.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(10619..11791)
                     /locus_tag="PNS21_001532"
     CDS             complement(10619..11791)
                     /locus_tag="PNS21_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDI5159245.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(11810..12682)
                     /locus_tag="PNS21_001533"
     CDS             complement(11810..12682)
                     /locus_tag="PNS21_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDI5159246.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(12704..13624)
                     /locus_tag="PNS21_001534"
     CDS             complement(12704..13624)
                     /locus_tag="PNS21_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159247.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(13624..13893)
                     /locus_tag="PNS21_001535"
     CDS             complement(13624..13893)
                     /locus_tag="PNS21_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159248.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(13890..14270)
                     /locus_tag="PNS21_001536"
     CDS             complement(13890..14270)
                     /locus_tag="PNS21_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDI5159249.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(14737..16200)
                     /locus_tag="PNS21_001537"
     CDS             complement(14737..16200)
                     /locus_tag="PNS21_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDI5159250.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(16264..19281)
                     /locus_tag="PNS21_001538"
     CDS             complement(16264..19281)
                     /locus_tag="PNS21_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDI5159251.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(19432..20271)
                     /locus_tag="PNS21_001539"
     CDS             complement(19432..20271)
                     /locus_tag="PNS21_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDI5159252.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            20416..20661
                     /locus_tag="PNS21_001540"
     CDS             20416..20661
                     /locus_tag="PNS21_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159253.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            20724..21146
                     /locus_tag="PNS21_001541"
     CDS             20724..21146
                     /locus_tag="PNS21_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159254.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(21240..21557)
                     /locus_tag="PNS21_001542"
     CDS             complement(21240..21557)
                     /locus_tag="PNS21_001542"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159255.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(21584..21790)
                     /locus_tag="PNS21_001543"
     CDS             complement(21584..21790)
                     /locus_tag="PNS21_001543"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159256.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(21771..21953)
                     /locus_tag="PNS21_001544"
     CDS             complement(21771..21953)
                     /locus_tag="PNS21_001544"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159257.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(21967..22317)
                     /locus_tag="PNS21_001545"
     CDS             complement(21967..22317)
                     /locus_tag="PNS21_001545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159258.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(22343..22645)
                     /locus_tag="PNS21_001546"
     CDS             complement(22343..22645)
                     /locus_tag="PNS21_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159259.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(22648..22881)
                     /locus_tag="PNS21_001547"
     CDS             complement(22648..22881)
                     /locus_tag="PNS21_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159260.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(23867..24189)
                     /locus_tag="PNS21_001548"
                     /pseudo
     CDS             complement(23867..24189)
                     /locus_tag="PNS21_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24312..25427)
                     /locus_tag="PNS21_001549"
     CDS             complement(24312..25427)
                     /locus_tag="PNS21_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDI5159261.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(25433..25831)
                     /locus_tag="PNS21_001550"
     CDS             complement(25433..25831)
                     /locus_tag="PNS21_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDI5159262.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         7249 a         5535 c         4112 g         9544 t
ORIGIN      
        1 ataaccttta aataatctct aaataaccct caaataatcc ttactaatac tgaaacgtaa
       61 aaaaaataaa cggatttcaa acaaaatacg tttatttttt ctattgattt tagctcaaaa
      121 tcgattcaat tcgagtgaaa tttgcttcaa ttttcaattg gtttttgttc aaattttgaa
      181 acatttttgc tcaaaaaatg agtgaatttg tttcaaaaga aaaacaaata cgatcgaaaa
      241 aaatttgaac tcataacaat tttttgtttt tttcaacgta ttctctttca attttcacta
      301 aaaattagga agaaattgat tcaatgtctt ttcaaaaaag aatgtttttc tctcaatttt
      361 tttgacgttt ctatcatgaa tcacgagaaa acttgggaca cttttaacat ggtaacgttg
      421 taaaaaagtt tgaagttctt cttgtcttgg ttcttttctt gtttcaattc gatatacatt
      481 cctagagaat tttttgtcgt atctttccaa gaaagattca acttcttggc attttttaca
      541 atcatttctt gctagataca caaaaaatga ttgattcgtt ttttgaagtt ctagtacttc
      601 ttctaaatta ctattgatta gttttccatt tgattcttcg ttagttttta tcagtttaaa
      661 acaaccaaat aagaggaaac taacaataag tatcctaaca ataactttct tatttcgcat
      721 attgtttctc caaatttggt ttccgaatat agacgactcg cggtggttcc tggacgtctt
      781 cagctgttcg atacaccaca aattcgcctg ttctcaatgc tcgaatgcga ttcggattga
      841 atttaaagcg gtctacgtct cgtttcgtgc ctctttctcc tgtccaatca acctttccca
      901 tatcagaata acctgcttct tgtgtcgtca tttccgttaa ttcaatgtct tcttctgtcc
      961 ccattaaatc tgcccaataa tcaatttctg tcgggtcatt tgtcttccca ataaagaacg
     1021 tattcacatt cccaatcaac tgttttcgaa ggacaggatc aattctatca atgtccgcaa
     1081 gcgtttgagg ggcatacgcc gcatgcaccc cagcgcctcg tcccttattg actaagtcta
     1141 taaggttatc atttagataa gaagctggtt cgtcaaatat tactaggact ttcgagcgat
     1201 tggctgtttc tcctgcttgt ttatacactt ctgacgcaaa gaaattaata tctcccacaa
     1261 gcatttgagc caatgtccga ataaactcac tataaatcaa tccgtttaac gagatataaa
     1321 caatctttcc ttctttaaca acttcttgaa tatctaattc tctttttcct ccagaagtat
     1381 caaagagttc accaagctca ctatccaata acatattgat attggtctgt aaattctcag
     1441 aagtcgcata caaataaaac gggttcttct tatgttcgta acgaacaaac aaccgtttaa
     1501 acaacgtttg ctcgatttct gtcatgagag aaatgttttc ttttaataat agataatacg
     1561 tttctaagtc aacggtttta cgatcatgtt caacgattcg ctcttcactt aactgttcca
     1621 catcggcaaa atcacgttct gttttgtcca tcgcttcatt ctttttcgtt tttttcttcg
     1681 tggttttctt cttttctacg accacttttt ctttaaataa atctggattt ttcaactgaa
     1741 tacgatccgc aaacaaatgt aagacgtttc gtggcaataa ataatgttgt aaccaaacta
     1801 gatttctttc aattttatgg ctcttttgga actcgtctaa aagctgaatc gttacctgta
     1861 ataacgcttt ggctgctgtc gaataataca cgctttctgt ttctgctaga ttggttaatt
     1921 tatctcgaat ggcaatatca ttgccatttt ttacaggatt ataacgcaaa tcgccaccgt
     1981 ccacaaagac ttcaatcggg cgattgtatg cctctgccac ttcttttaac gcttgtaaag
     2041 tctctcctgc tcctttccca tcgactaaga taacggggat acgaaactta cacgcatgtt
     2101 ctgctagtaa ttggatcacc gtcgtttttc ctgaacctgt agtcgctgga ataatcacat
     2161 gttggttcaa ttcactaaag ttcatcacat aagggagccc atactcatct gttcctaata
     2221 gaatcacttt ggctaatgaa gccagttgtt tattccgcaa agcaacgtct tttatgacaa
     2281 ttgctacctg gtacgcttgc tgatactctg ctgtatattt ttgggactcc tgaaatttat
     2341 tcttccgacg ttccttgtat tctttactat ttagcaaagc tgtttgccgt tctttctttg
     2401 aaacaacttt tcttttttca ataaaagacg catacgcaat caaacaactt cctgcacctg
     2461 tcccacctaa aaggagggca aaaatcgttt gtccattaaa ctgcaacgca tgagaaaaca
     2521 agtttaatgg aaataataag taacacgaca aataccctct aggaagaaca aaaataaaac
     2581 aaaatagatt taatagcgta aaacctaacg caaaaataaa aaaaagagag cttcttcgtt
     2641 ttaagaagct ctcatactca aagtcataaa tatgtgcacg tgtctctttt ttatctatca
     2701 taaagacaat cgctaaagga atactgccta caataaaaaa tggatataga caaacaccta
     2761 ataaagtgac gataaaataa agttgttgaa accgactttt tggcgtttct ttttcttgtt
     2821 ttcttttgcg atcgacagaa aacatttgct caaaatgtgt cggtcgtttt ggtgcattca
     2881 tcatactatc gatttcctcc tatcgttttc gttgtttcat ttctcttcga taaatcggtt
     2941 gtttttctcg ttttcgtttc atttttgctt gagttgctcg aaactcactc cgtgtcttcg
     3001 gtgcttgttt tggtgatttt gtttgtttca tggttgagta tgctttagat acggttgaag
     3061 gcgctttggt ttgtgctgtt actcgattga ctgctgacat atgttcccga accccctctg
     3121 gacgtcgaac agatttcggt gtggtagaac ctgttgctcg tggtacattc ccttttctta
     3181 gtggtttttg taccgcatgt ttttgtcctg caactgtccg ggttttcatc gaacttgttt
     3241 ttgttacggg agtaagtgcc tccttccgac gacgacttgt ttgtccctga cgacgttttc
     3301 gatcgttcaa ttctgttcta ggcgtatgct tctggccata agacttgtgt ttttgctttg
     3361 gattcgctaa tgacaccacc attgcagaac cctgattgcg atacggacta cgagacgcac
     3421 cttttctact tctttctctt agactaggat tgccttgcaa ttctcgttgt aataaatcgg
     3481 ctgtttttct tgcctgtttc tccattgctg ttcgttgcat acgttcttga ctttgctctt
     3541 ctgcttgttt cgcttgatca tgcaagtctc ttgctttttg tgccatacgt tccatacgat
     3601 ttggtgcttt atgacgaggt tgttgtgcgg attcacgccg aaaagcactt cgtgtggctt
     3661 gtttcgccgt ttgtggcata cgaacgtctg agccaccccc acgttttcgt tgacgttctt
     3721 gtctcttccg tgcgttttct ttttcttttt gtttctgtgc ttttctcgct tctttgcgtt
     3781 ctttcaaccg ttgtttggct tcccttctca attttgcttc tgccccaaat ggatttcccg
     3841 caaaagctaa cgctcctcga acacccattc ttccagtaaa tagactgcct aaaaagcctc
     3901 ggaacaaata aattcccaac ggcgcaaaca aataaacaat gatcaagaga attttttccc
     3961 atggatcgtc ttgtgtacta cttaattgga acccgtaact aagaaaagaa gaaaaaagga
     4021 tacatgcaaa acctgtcatt ccttttaaaa acgtcaaaat accaaaaact ttggcatagt
     4081 taccaaacac attcattgtg acaagaaaca atcctaaaaa aagtaagaac ggcaacatcg
     4141 gtactaacaa tagttgcata gttccaataa tcaatcgaaa catacttaag acaaaataaa
     4201 caacggtttg aatcagattc acgacaaaat aaaacaaggc aaatgctgta tttttccaac
     4261 tattgtaata ttgtatgttc ttgtttttca gtgtatctgc ttcatactct gtgactttgt
     4321 catgaatttt ctcccctatt tttgtaccta aatcattgtc caataaaaga ttgattcgat
     4381 catattcttt tccagcatat ttgactttct tctcacgaat tgtgtctaca ttggttgtgc
     4441 cataatttaa caacagatat ggttcataaa cattactata aaagacccga gccgccatca
     4501 atttgcctgc cgtttccact tttttatccg ctgttacctc ttctgtttct tttgtttcat
     4561 aatttgtttc ttcttcaggt gttaacactg gattgacctt cacgaacgcc gtttccactg
     4621 tagaatctaa agaaaaaaga acatcaaaaa gattcttatt tgtcccagaa tcttttaata
     4681 cgcttaaccc agtaaaaata aggatcgtca taagaaagac acggagtgct tgtttcattc
     4741 gagcctctcc agcatatttg attgccatca taattgccga aaaactgatt ccaatagttc
     4801 ctgcaatact taacatattg tccgcaattt tttgtgttaa gttttgaatg ggttctcgaa
     4861 tggcggaaat aatatctaat tcaaagagaa attcaaccat tgaagcattg aattctccta
     4921 acatttttac gccactccaa acaaaatttt tcatcactcc tgcaacattg tacgatagct
     4981 tttttttaag gttcattgac tcatagtcat tggtcatcaa ttcaaacgaa ttctttttgt
     5041 aatttttata caattgatcc acatcagtcg ttacttcttt tacttctact ttatcgttgg
     5101 cattctctcc ttcagaacga tcggtttcag tgacacccgc accatcattc atttgatcgg
     5161 cagaaacagt agccccttgt agacagaaaa aaagaccccc taataaaagg agcgtccacc
     5221 atttcagaga tttttttcgc aaaaaaacca cctctttttt gttttatcgt tgtttttcat
     5281 tttctaacat tgcttgaatt ttttcatctt gattttcagt agaagaaaca gccaataacc
     5341 aactatcaaa caacacatca atggcaatcg gttgatttct tcccaaatga tcctggaata
     5401 aacaggttcc tgtttgtaat tgttccatca tttctaaatt ggctgaattc acttccatat
     5461 caaagaagga caatagtttc tctcgtgctt ctttttgttt cggacgaaac gcaaatttgt
     5521 aagaaattaa ctctttttta tcttcttgat cgtaatcttt aaacgcttgc acaacaaggt
     5581 aaatatccgt attattcgca cgtccttttc gcaagctatc atcaatcaaa tagcttcctt
     5641 gtgcggtatc ttcataccct tttgcctcgt cataaataat cgcagcgtct tcttctttgt
     5701 ttgtagaaaa gatatacgta aacttcataa tgacttccat aattgccatt cctgcaagtt
     5761 gttcgctatt taatcgaccc gaatcaatct ctttttgtgt cggaatgcgt attccttgtg
     5821 tccctaacac gttgatttgt gaatcaaaac gtatcggcgt actgtgctca ttcccaatga
     5881 gaatctttcc taaaccaacg ctatgcccta aaatcaattt agctagttct ttgtcacttt
     5941 ttcgaatttc ttcgataact tgacttaacg aatgcttacc tggcatggtg taaactacat
     6001 cgatggcgtc ttggatcacc gttttcttgt ggctcgctgt ttctcgattg gtatctactt
     6061 ctcctaacat tcctaacaca tttttagcgg tttgaattgc ctcttctttt ggtaaaaaca
     6121 atagaggatc aagcatccct cgacagcttt catctgaaga taacgaaata aaattaattc
     6181 gttcatacat agccacaaac tctggtatgt gtccgaattt ttcaagtgcc tttaaaaaga
     6241 acttttgggt ttcatttttc ggatcaatat aaagaatttt ttgacctaaa aacatcagcc
     6301 ataaaaaaat atattttaca aggaccgatt ttcctttacc tggtggacca ataatcaacg
     6361 tatttccatt ggtatgttcc gcgccttcaa tacttttctt tgttaaattg ggataatacc
     6421 atactagttt actagatagc gctaacgctt ctttcgcaga tttaaacttt tttgcaataa
     6481 caatccgtcc taaaggaaat cctttcttat ttcctacttc tttctttaag tccaagccat
     6541 aatctgccac aaaacctgtc gtagcgacct gctcgtaact acgaaaacgt tgcgtacttc
     6601 caattaagca ttgattaaac aacgttagct gatccgctac cgatcgataa aatttgaaat
     6661 ctgtagactc aatcgcaaat tcaatatctt tgactcgttc ttccaattct tcttttgaag
     6721 aagcagatac aaccagcgtg acggtagaat aacacaatcg tttttctttg cgcttcagct
     6781 tatcttttac atccattaac cgttctgtac caaaatttaa tacatcgtct gaatctaaaa
     6841 tgccgtcaac ggtctcatat tctttttctt gttcataaat ccgtccagcc attttattaa
     6901 tttttcgttc atcagatttt gaatgctcaa atcgaagtcg aatatgcgta tcaattggaa
     6961 aagataagct atcctgaaga ttttgaataa agctactccc atacatggct aaaggagcgt
     7021 ccgtttgact taaaaacgtc gaataatgcg tttgattcaa ctgttcaatc gcaatgtatc
     7081 ctgcttcatt cgaaatgacg ccctcgcaaa tttccattgg cgtaagctgt ctttcaggca
     7141 atttttcttc tgatcgatgg aaaaaataat aataaatttg tccaatctct tgggcagata
     7201 agcgctctat cggcttataa ttggacaatt ccgtatagag ctccttttct aatgtttcat
     7261 acgttggtaa cacactactt ataggaactc gttgtcccgt aatacgatag ataaactttt
     7321 taaaaacatg cttaagcaat tgaaaatatt cgatcggatc agacactttc atttctgtag
     7381 tgaaacgcac aaacagatac gttcgatact cgtttaattg cacttcgtct tttaaaatct
     7441 ttcctgcctg ttcaaagtac actttcccaa gatctgcgca ttctcccttt accaaatctc
     7501 gaatggtttc ttcaatgtgc gtatctaggt taaattgacg tggaatattg ataaaatgat
     7561 agtcatattc atcatgtcca agtatgcctt cgccatcttg aagatagttc ttgaaaaaat
     7621 caatgtcatt taaaggaaat acttgggaag aaagttgata gcctacccac acatcatcat
     7681 ttttggtcaa aacaaaattt cggataatgg cgtctatcgg aaattctaat cgaggaagct
     7741 tcgttttttt ctttttgaac tgccaaatcc cgataataaa gacgaccaaa agaaacaaaa
     7801 acgccattta ctcgactcac tccttccatt tgtgtaggga atacgtcact ttttcatgga
     7861 aaaacggcac atacatttca tgttcaaagc gttgtttccg tttctgataa taggacgttc
     7921 gcccaattaa atactcgaaa aagtgtttat tgtcccattt cattgaaaac aatgtccaaa
     7981 cgagaacacc aatcccaaaa atcataatca accaggactt tcgaaacagt gtctgcaaga
     8041 aactaatttg attcacatac gcaatcaaaa ataggatcaa aagaatgaag agaaataccc
     8101 cacctatgac taattgttcc atgcgaatgc cattagaaat ccagggaatt ttttgtcctt
     8161 taatcccgta aattttgtga gggtgcttaa actcctttgt atagttgtat tccatgttca
     8221 ctctacctcc ttttacaaaa aaagaagcga ccagaagttc taatcttctg atcgcttttg
     8281 cagtttgtct ttttcttatg ataattgtat ccagggcaca atcatttgcg ttacccccac
     8341 gttctcttca ctatccttaa taaagataat gttctttagc cattcaccaa tatcaacggc
     8401 attcgataac aataagacga gcattatcaa aataaagacg ccaataatgg caaacgctgc
     8461 ccgcgcaaag ccttgggtcg cataagcacc aaccattaga acaatcgcaa cgattgccaa
     8521 tagccaaatg acttgtccag atacatcatc aaataacgat ttaagatcaa aaagtttcat
     8581 ttccattctc ctctaagttc attttattca tttaacgtag taacaaaata actatcgcca
     8641 cttgttttta cttccattga aaagtttgat tggaactcgt ttcctttctc aaaatagaaa
     8701 cgatacttcc cttgcaaaaa gtagtttcct tctttatttt ttactgcatg atccagttgt
     8761 acattgatca attcgccgcc ttctaaccct ttttcgttag aaatcaatgc caatttttca
     8821 tcatttttga catataactc aaaaaaccgt ttcacaaatg cttccagctt gatttcttcg
     8881 ctttcagtga ccgcttgccc agaagaataa aatcgatttt cctgatagtt cacagaattg
     8941 gtattcttac tctcagaaag attcaaaaat tgaacgccat caagtacttt taaaacattg
     9001 ttttgataac tgactggaaa agaaagttga ctgcttttcc aatgttctcc ttctgtaaaa
     9061 gtaacctcat aaaataggcg atacattgct tgtgtcaaat cgatttcttc tagtttaata
     9121 aagcgaacat ctttcgctac tcgattggta gccgtcgtta caaagcctag atcagacgct
     9181 tccaatcctt gtgctaaata atgattgacg gaagcctccc attcgtttcg tttttctggc
     9241 gaataagtaa ataacctttc aataaaatgt tgtccctcgt acttcaattg ttccgtagaa
     9301 gacaaggttt ggttgacttc agttaacaat gtttctggat tgacttgttt tttcactgct
     9361 aaacgcgtta atgtatccac tcgtccaaag tttaggagga gcacgacacc cattaacaga
     9421 aacaaacaaa aaaaagcgcc tgaaaataca ctcatggcac ttttttgcga aaattggatc
     9481 attcgatctg acggttctgt tttctttttt tggaacaaaa aacgtttctt ttgtttcttt
     9541 tccttctgtt gtttcggtgg cttagtttgg aacgcttgtg gattaaattt tgcattactc
     9601 atgagaaatg ccataccgtt ctaacaataa tttcggtatt ttgtccacaa tcactacttc
     9661 gtggacaacc acacttgccc cctccaaaaa ggcaagtgct tctttgtagg cattttgact
     9721 gtattttttt gataaagcat gacaaatttc ttgataaaat agttgactgc ctaccgaata
     9781 ccgattattg tgtatccgcc acaaaacgaa cacactgatt tgaatcgacg ggcgtaaaaa
     9841 agaaaaattc tttccataag aaaaagaacg tccttcttgg gtttcttttg cttgttctgt
     9901 tggtacttct tgtacctctc ttattgattc ttgtctctcc ataaccttct ccttttctta
     9961 ttcttctaaa agctgttgat taaagctact tcccaaattt tgcataaacg tgattttctt
    10021 tgacgctgga tcaaaggtta cgtcaaaaat attcatggca ttattccagg aactatcacc
    10081 aattttattg cgatacgaca ccacaacgag cccttggata gtcgcttttt gtgtgctttt
    10141 taaataaaca gaacatttct ctaattcaga agacaccccg tttgaatcac cataggaaac
    10201 gccttctttg aaatattgct tcatcagttt gtcagaaaga tatggtgcac aattgttttt
    10261 tcgttcttga aaagaatcag gctcataatt aaacagaacc tcgaacgctt ctgtcactgt
    10321 tttttgaaag gtttgaatgt ttcctttttc tgttgaatca ttgtttttca attcttcatt
    10381 tgcttttttt aacgtcgtga cctcgctttc tacttgttga agttgttctt tgagtgacgt
    10441 tacaaggtct tcctgtcctt tcttctcttc ttggaacttg tttatctcca ccgtcagttt
    10501 ctcttccttt gctgttagct gtagcttttc atgaataaga aaaaatgtac tgcctacacc
    10561 aaataagaac aatccgccta cacaaacata aataaatcgt ttcttcattt gacacctctt
    10621 atttctttat cggtcgagca aaacaaatat caccaccatt aagcaaatac ccaaaggata
    10681 aattgacttg cccttcacct acaccgttag tattcattcc cccttgttcg attactttcg
    10741 tagaaagtcc atgccaatct tccgctaaaa cagctgtgtg tccatcattt cccacaccag
    10801 aacctaagtt cacaatgata atatcgccag cttttgcctc gctctcaggt atttcttgta
    10861 agtattgctt acttcctctt gcgtcgctcg tcattgagcc ggtaaaccac ccaacgttcg
    10921 caggcgtttt ataccctgct ttcgttaaaa caagccacac aaagctcgaa caatcggcat
    10981 agccattttt atctggattt tcgacactac caaattgtgc ccgttgcggt tgtgaataat
    11041 gaaaataacc aatcagactt tttgctacgt ttaatacatt actatcggaa gaactgcttg
    11101 ttcctgttgt ttctagacaa taatcggtca agtctttact tcctgcgttt gccccatttt
    11161 ctaatgtaat tgaagcgacg ggatcactgt cattctttcc tagttttgcg taccattgat
    11221 tcgcaaaagt aatacgattc cccatcattg gtatccctgc acgctcaaat ttcacttgcc
    11281 actgttctgt tgcttcctct acgctttttg cttgtaatgc aacttgtgaa gctttttctg
    11341 ttgtgtttag ttcatgaatc aaaaactcaa cttgtacacc taaatctgac cactctttgt
    11401 ttcgctcttt ggcaaaattt ttcaaagcag tcgttcgccc tgccgtccat tgtgcaatcc
    11461 catgcccaga taatggatcg tttggtcgtt caatcgctgt gggactcata ctcgactctt
    11521 gttgaaaatt gcctaacata cccgctagac cctctaatgt cgtatttgga atatgttttt
    11581 gtacttcctt tgcaatttga agcccgtttg tctccatgcc ttggttcgtt aatgagatag
    11641 tacttgcgac aatttcggtt ggactacagt cgatttcttc ctcttcagaa ccactactaa
    11701 tcaaaagtat cataaacaga aaaccaaaca tacatagtaa tagtgtgggt agacaaccga
    11761 acccacacca taaaagaagt tttttcttca tgtttgatcc ttcattcgct tagttcattt
    11821 tttgtgttgc ttttcttttg cgccaaaaag caaaaccgct tcctgaagcc aataatccac
    11881 caagtgctgt cgcaacgaga gacacttctt cgccagtctt aggcaatact ttcatttttc
    11941 catcagaccc tttcaccgca atatttccgc ttggaagttg ttcgaccttg tctgaaatgg
    12001 gcgtcaagcc atttcctgat tctttataag cgacaccatc aacgacggag acaatttttt
    12061 ctcctgaagc tgtttcaatt ggtgtacttg gtgttggaat atgggttaat tcagacgcat
    12121 tattggtttc aatttgaaca ttttgtccac ctgtttgttg gctgccaata cttccatctg
    12181 gttttactac gacgtcctgt actggaggcg tagtgttcgt ccccccatta ttggtaggtg
    12241 gtgtcgttgg aggagtcgta tttccttcat tgcctcctgg attcgacggc ttttctggtg
    12301 tctctgtatt accaccattt cctgggtttt cctcaacggg tggaacttca ggtgttcctt
    12361 cccctccgcc agtatctgga ttttctggta cttcaggcgt ttctgtgcct ccacctgtat
    12421 ctggattttc ttccgttggt ggaatctctg gggtttcagg agtttctggt atttcgggaa
    12481 tttctggggt ttcttctcct ccatctgttg gtggtaaaac aggttcttca ggaacttcag
    12541 gtgtttcttc cgttggtgga ataatttctg tcggtggtac acttggatca cctggtgacg
    12601 tagtatctgc atacgcactg gttcctacca tacctagcaa tacagttgca ctaaataaac
    12661 ttcctaaaat catttttttc atgtgttttt cctcttctct tatttattga tttatttcaa
    12721 aatcatctag ttcgggaagc gtggaaacta gataattttt ttcctgttct tccaattgaa
    12781 aagacacttt ttttgtttct tgtgtctttt tctcggtttc caatgtaatg acatacgcaa
    12841 tttgccaatg cttgccttct ttcttcattt cccaggataa aatacttttg actttttcct
    12901 tttgaggact gatttgtttc gcaacttttt ccgttagaaa cgattgaagc tttttcctat
    12961 accgttcttc tgccatttca cccgaataat aattcgtaat aaaataacga ctaaatgtat
    13021 caatttgtgg ttgttttgtc gcaagttctt ccgcaaatgc aatgcgttct tgtaactggt
    13081 gctccttttt cgttagttgt tcaatctctc tttgcgttga aagttgcata ataaaaagac
    13141 cacaagatac aacgacgata aatccaaaca aaagccattg aaaagacatt tttttcttct
    13201 gaagagagcc gttgctttgt gtaaacgctg tttctacaga aagtggtgtt ttcttttcct
    13261 ttttgatttt tgttggtcga cgttcgttct tttggtcttt ctttacttga taaaacttcg
    13321 gtaattcgga atttaaggtt tgaataaagg tttgtgcttc ttcacttaat tgcccatttt
    13381 ctgttaaaga ttgttcaatc aatgaaatca aatgaaactc ttctactgga aaatcatacg
    13441 agccttcaaa tactggaatt tcatggtttt ctgtgtagat ttccaaggta caccagccaa
    13501 tttccgctcg tgccaatagc tcttcatttt ctcttttcaa ttgaataaat gccttatcta
    13561 atgcaatttt tttatctgtt agtaaagaca tgttatcaac ttctgtaaat cgaatcatca
    13621 tcattagtca aacacttctt ccaccacttc taccgcatac accacttgaa tagggttatt
    13681 taaagaaaat ttcacccgat attcttgatt gttcaaactg acgatatagc tatcctcatc
    13741 ttcagcataa taggtatatt cgttgccttt gacttgaaaa tatttttgga tcgcttgtct
    13801 ttgttgccat tcgccaatct ttggaaaata ggcgaaatga tagccaaata aaacgcctat
    13861 ccctaataaa acaattcctg ttactataat catctggttt cctcctgtac ctgtttatct
    13921 tctattgact cttttgtcgg taagcaataa agaaccagta acacaagaga gccaatcgtt
    13981 gggacaaaat agaaaaaaat aaatgcccaa tgaaaacctg cgtctcgtaa acgtcgaacg
    14041 ctgagagaaa gagacgggac aagaactacg aaacaataca caatcaatac gcctatcaaa
    14101 caaccaatta ttgtttcaga agcattcaac gctcctgcta gtccaatcgc taagcctagg
    14161 ccaaacccaa tgagggcatt cattaacgca acgagccaat actcttttct tgtggctttc
    14221 cctttgtaat taaagctatt ctgccaaaat tttttataat ttgtgatcat atctttgtcc
    14281 tttctaatgt ttatttttga ttctagtttc ttctgtttta ctaacaatga aacttgccat
    14341 aaaaaaggtg gccaatacac caccagctag aaatccactt acaaataaca aaattttttc
    14401 ctcctttcta aaaaatttcg ccataaaaag gcaaagtatg aacacgataa ataccatgta
    14461 catactttgc ctttttatgc aatatatgtt aactctccaa gcaattactg atttttaaat
    14521 tctttataaa taaaataaaa atcgtttgag taaagaaata agtcaatgaa gacccaaacg
    14581 ataaaaaata ttcctaatac tatatttaat aaactacgtt ctagatagat acgagatttc
    14641 aaattaaaaa acgaaaatga gagatacaaa tatatcaaat atttaaaaag taaccacatt
    14701 ttctatacca ctccttatca gaaacagcct tattctttag aataagactg tttctgggtt
    14761 gattaatttg tcttttgtta aactaccatc taaggcaagt tctaaatgta aatgtggtcc
    14821 tgtactattt cctgttgaac ccacataacc aataatttgt ccttgttcaa ctgtttgtcc
    14881 tacgttcacg caatattcct gttggtgcgc ataaagagcc acacttccgt cttcatgctc
    14941 aattgtgaca taatttcctg acaaagtatg atacgaagct tctatgaccg ttcctgcctt
    15001 gctcgcatag attggttctc cttgattggc tgccatgtct aatcctctat gaaattcttc
    15061 tcctcgccaa ccgaaggaac tagaaattga ataatttttt agaggtacca tatagccttc
    15121 tttgcttagt tgtggaccag ctacttcttt atcatatttg attaagtcat aggtttcgat
    15181 taacccattt aattttttat cgtagctagt gtctgttgcg tatcttcctg ttaaaaattt
    15241 agtcgcttcc tgataggttt ttgccttact tttccaaaca ccatcataaa aagaggtatt
    15301 tccaactaag ccttctttta acagtttggc atagtctttt aagctttctt catagctctc
    15361 atattgccga aaagttgctt gggtcgtata aagtgtccca ttgcctaaat cttcttgtgt
    15421 cgcaaaagag acgccctttc cgttatgtgt gcctttgatt ccaaatagat tgtagtttgg
    15481 tgcctgagcc aattgacttt gtccgcttgc ggattctaaa atggcttgcg cgatcataac
    15541 agaagcgtat aaatcatttt cttgtccaat tttacgagca gattcaccga ttttacggat
    15601 aaacgtttct acagacgcgt ctttttcaaa atgaatagtc ccttcgttat cttgtgtgct
    15661 ggaagggact aattcttgta tcggtgcgct tggtgcttgg tggtagtttt ttgtctcttc
    15721 tgaaccgatt gattgtgcct gcggtacgtc attcataggt acagtcacac tctgttcagg
    15781 tgccttttgt gggcgttccg tttttgttgg cggttccgct tctgtttcgg tacttggttt
    15841 ttgaggagtc tgtgagcttt catcagaagg ttgctcattt ggttcttctg acggttcctc
    15901 ctcaggttgt tgattgtctt ctggtttgtc tggttgattc ggtgtttctg gttgttcttc
    15961 agaacctgtt ggtggagcgt tcatttctgg attatctggt gtatctattg tatcttcagt
    16021 cggtgtttct ggttgatttt cttctccagt ggaatttgtt gtcgtttctt cagtgatcgt
    16081 tgcagattgc gaatttgtca gttgattgtt gccttgttca ttagccaaaa ccgctggcgt
    16141 atacccaaat acttgaccta ctaataacaa agcagataaa catttactcg tttttttcac
    16201 aagattgcct ccattcttca atcaatcatc tagagacagc tgattgctgt ctctagtcat
    16261 actttaattg ctttgtttca ttttacgacg aaccagaaca gcccccacac caacaaaaag
    16321 aaacagtccc aatgcaatta acacatcgtt cactgtttct cctgtttttg gtaagctttg
    16381 ttttggtgtg ctcgtcggcg tttgtttgac aggggttgtc ggcgtttctt ttttaatgcc
    16441 ttttgctgtc aatgtttgtt tttcattctt caaatcaaag tgacgtgctt cttcctcttt
    16501 atggtcttta tccttataga caatatgagt tgcgaccaat ttctccccgt cttttaacgt
    16561 gcctgctttg tagtcaaagg atactttaaa ctcttcagac gctttagaaa ctttacgtgc
    16621 agtttctacc gtaccaacga ctgtatcttt tccattttta tcaatcttat ggaattgagt
    16681 cacccagtat ttggtttccc ctaccgtaga ttctggaaat tcttgcgata catgatcgat
    16741 caactcttgg tctttgtctg gatcaaatgt ttggtcgcca ttcactgtaa taaagcgtgt
    16801 ttcgattttt ggttctttcg cagtcactgt ttgatcttta ttggttaaat caaaatgacg
    16861 agcgaattct tcttcctctt ctttgtcgtt atagacaatg tgcgttgcga caatcgtgtc
    16921 accgtctgaa accatgtctg ctttgtagtt gaacgtcaca tcaaatgttt ctttttcatc
    16981 ggtcactttt cgtgtttctt ttaccgtatc taatacagtg acttttccat cggcagtaat
    17041 tttatggaat tgcgtcaccc aatatttctc tgtcccaacc gcagatttag ggaatacttg
    17101 ggacacttta tccactaatt cttggtcttt tttcgggttg aaatcttttc cacctgtcgt
    17161 tgtcacaaac aaagtagaaa tttctggttg gtcttcttct ggaaccacga tttcttcgtt
    17221 tttcacagta attggttgta cttctccgtc tttgaccgtt actaaaatcg tttctgtttg
    17281 tcctgacgct tgcccataat gctcagggaa cacaatttcc gtaaatttat acgtaccgtc
    17341 tggtaatttt agtttttctg gttcttcttt tgtttcatac acaaaggaca catttttatt
    17401 atgtgcagtc ataccttcaa tcaggaattt cgctcctggt aattgtagat gattgattga
    17461 atcgacttta ttgaagtcta cttcacccgt ttttaacaag ttgtcaatca catctaattc
    17521 aaacgtaagc acaggcgtcg tgttatcgcc ataggccaca gagaacgtgg tttgttcttc
    17581 agaaggttga cggtagttgt ctggcacatt tttttcaatc aattggtatt tgttttgatc
    17641 gccattgtag aattttttgt ttgccttcca gtgtccgtct ttgtcagttt ttactgaatc
    17701 caagacttct ccttttttca caaggactgt ttctttatct ggcgcagtaa tatctgtttt
    17761 ggcgataata tcaaattcga tattttctgc aggaacggta tcataaacag gtttgccttg
    17821 attccaggat tcttccgcat tatagacttc tttgactttc tttccttctg gaatgaactg
    17881 ttgtaagtca ttttttgcag ttaacgtcac ttctttgttt ggttcaatcg taatcgtatg
    17941 agtttgtttg tctagaatat acccagctgg tgctttgact tctttgacat ccaatttatt
    18001 ttgaacatat tttccgtctt tttcatagaa tgcagggatt tcttttaagg cgatttcccc
    18061 tttggcatcc gttgtatatt gttttgtttc tcccgtatca tgacgtttga tttcatacgt
    18121 cacatttgga acttttttcc ctgtgacagc gtctagtttg gttagtttga agtcgccatt
    18181 ttttagaact ttaatagtta gtccagctct attaggatct ggcacataaa gctttcctag
    18241 attttgagca acaggatcta cataggcaaa acttgaacct acccctctag aatttggatc
    18301 cagtgaagaa acagaaattc gtccgtcatt tgagttttga tttgctttta taaacaaact
    18361 atttcctgat tttcttactt gaacgttagc tgtgttttca acaaccgacc catcccattg
    18421 agaaaacaca ccttttgtat ccttaacttc tacttcttcc cctactttaa ccgtcactgt
    18481 ttggctatca aaagaaggat cttcaagtaa attgtttatt tttctatcta cagtctgttt
    18541 gaaagcttca taattagatt gtccaatagt tccataatta acaaatgaac cacctaaaac
    18601 ttcccaaata tatgtttgtg ctaatacata gttatccatt gaaccactag aatagtagcc
    18661 aaaataacca attaaagaaa tcttttttct aatagcactt gtcgtaatta aattttcgag
    18721 tggtgtcaca tcatattgaa tacccgcact cgtgattgcg ccttcttgaa tgcaaaaaac
    18781 atcttctccg tcaatttgcc agcgaaccat gtaatcatga gtatacattg ttccttcagg
    18841 agtaattcga gtcatattca catctttacg accattttca gtcaatgtta gattggtagc
    18901 acgtgtctgt gcattctttt ccaagattgc ttgccattct tcagattctt gcgccaatcg
    18961 ttcttgttca gaaaaagaag tcgctttttg aatatcttca atggttataa agtatttctc
    19021 ataaccttca gggacctctt gagtcattac attgggcgga gaaacatctt ctgtcgtgtc
    19081 ctgcgtagtt gtctcttctg ttgtttcact tgtggtttca gtactgatcg cttcttcact
    19141 actttttgat tcttcagtct tcattacttc ttcagtagaa tttgattcat ttatcgtttc
    19201 tgcaaatgcc gttacagatc ctaacatggg attgagaaat gtaacgaaaa ttgccaacca
    19261 agttgccatt cttttaaaca tatttttttc gttcctttca ttttattttt cagtcttcta
    19321 aagtaatttc ttatggactt cgacaccgaa acatttttat cctcctcaaa acaaaaaaag
    19381 actagaactt ataattctag tcttcatcaa ccacttactt ttcttttatt tttagtagaa
    19441 atctacagtg tgtttttgtg acccgtcact gtaaataata acatccacca taaaaccttt
    19501 gttgtaccac tgcgaatttg gatcgctaac ctctgcatta gcccaatcat aggcttcatc
    19561 tacagtgtta aatacctttc cactattacc aatctctcca actacaattg ttggctcttc
    19621 aggtgtcgtt gtgccaccat ttccgccttg atcgcctcca ccaggattgc ttggttcttc
    19681 aggcgttgtt gttcctccac ctgtgcttgg gttagaagga gttgtttctc ctccagtatt
    19741 tccaccatta cctggttgtg taggtgtttg agtagtgcca ccattgtttt ctggtgtact
    19801 tggcgttgtt gtaccaccag ttgttcctgt ttgatctcca gctggtgttg ttggtgtcgt
    19861 tgtatttggt gtcgtttgtg tatttgaagt cgttccttgt cctactggtt gattcggatc
    19921 aactttgtct ttctcttgaa tcactgtttg agccacagtt tcacctgcgc tgttttgtac
    19981 acttgctaca ttgccattaa atactaattt atttcctgca tagattaaat ccacatttgt
    20041 aatcttattc atgtccgcta atttttggac agtaatattt gttgcttgac tgattgcgct
    20101 tagggtatct ccccagacaa ttgtgtattc attcccttta atttctgctt tgatttgttc
    20161 cgccgaacga ggtgtccaaa ctttgttttc tgctgcttca gcattcgttc ccacagcaag
    20221 actaactcct ccgactacga ttcctagtgc caatactttc aacattttca ttgtttttcg
    20281 cctccgattt gaataaattt atttacaaaa acatagtacc aaacttatgt ctatcagtcg
    20341 atatattttt tgtagatttt tttatttttt gatatactaa ctttggtact aaaataacta
    20401 aaaggaatgt cacacatgca acttaataat ttaaatgaaa tacatgattg ggctcaaaat
    20461 aatttactta atcgtaaaca agctgccaaa atcactggac aaaactatga tgctttttct
    20521 agctctttac gtagaaataa tatcaaacca tttctagaaa taggcgacga acctggtcct
    20581 tccatcattc gcttatatct aagagaagat attgaaaaat acgcaaaaca gctcgaagaa
    20641 aaaagacaga aacgtcaatg aggcgttttc tgtcttggat ttacaagtaa aacaataata
    20701 aacactttta caacggaggt tctatgaata agaaatacat tgtttcagtt ggttcaatcg
    20761 tactgattgt actcgctatt attgcttttt ctgtatccaa caaaacagaa acacagccac
    20821 aaaatacggt tcaatcgact actacaagtc aatctagcga tcattccgtc tatccttcac
    20881 taaatagttt aaaagaaacg catggacaag atttaattga tcttggttct tctcaaatta
    20941 ctactgtctt atttgaaaag aaaaatattg cgtttattac tcctaaagaa ggaactgacc
    21001 ttgaattttc tatcaaagta acaaatacca aagaaaattt attctccatt cctgacattc
    21061 aatatacccc taacaagtta aagattgggg atacgtttgg tttggctaaa gtaaatgaac
    21121 aatactttgc ttactcactg aaataagttt ctaatagcaa gaggaaaaac cctcttgtta
    21181 tttttatcac ataatcattt tataaaaacg cttcgaaaat atcaacattt ttgaagaaac
    21241 tatttttcta atttctcaat ttcttccaag ggattttcgc taatgaccca tacaggcaca
    21301 gattgctctc gaggctcccc ttcttctaac caccaacctg cataatgaac ttcttcctcc
    21361 tcagcattac catgatacca accaacaaag gattcatcta caacgaatga aatttcttct
    21421 tctggatcaa ataattcatt tttccatgct tcaattgctt gttctttggt gtacttttgt
    21481 ttgttaaata caccacaaat ttcgtcatct acataaaaaa tttcaaaatc aaaattactc
    21541 gttcctttta ctggcatctt aacttttcct cctttgactc acttcaatag atataccact
    21601 catgttcatt ccattgttta ctagttaatt ggagttttat cttagggctt tgtgcactct
    21661 ttccaaaaac ttgagcgtcg tttttttctt ctaaccaaaa aaggtaattt cttacattcg
    21721 gtactaaatg tttttcctgc atttcttcat gaaaaacata taattctgct tcatttttca
    21781 accaattcat tattcattcc ctcattcact tcttcatacc gccattcaga tagtgagatt
    21841 tctttttgtt tctccacttg tcctataaaa atcgctgtat tcaacaagct gatcacactt
    21901 aataataaag caatgaaact aatggttaca cttactcttt cacattttgt cattttgttt
    21961 ccttctttac tcgtttgtta tttcttgtaa taggtctaat aactccttca ccgaaatttg
    22021 caaatggttc gcaaaaattt caataaaagc actcgcacac attaccagac cttcaactga
    22081 tatatcactg cgacaataaa attctggaga aacactgatt tctgattctt tcttacaatt
    22141 gaacgaaaaa actatttttg aatcaaattc cggtaattct tccacaacta tgttttggga
    22201 ttctaccaag aaagggagtg cctgttctaa tgcttcttct ggtgtttgag cagatagaat
    22261 aattgcttct tgttctttta tttcattgtt ctcaaatact ttataacttt ttttcattgt
    22321 tagttcctct ttcattcctc tctcaattag ctaagccgtt actggtacgc cattctctaa
    22381 atttagtatc gctaatcctt aatgctcgtc gaatatcaat cattcgataa cctattgcac
    22441 gaagttcttt atatctttta gcagttagcg tctctaacgt catttcaggc aatgggttcg
    22501 aatcaaaaat agaattcgaa ccgattattt gggccaattg ttgtacttgt aacagctctt
    22561 ctgaacttaa ttcagtgatt gctcgattgc ctatcattgc atacattttc tgtcgaatat
    22621 acttcttttc ttgtttagac aacatttcta tccactccta ttttcattat catttgttac
    22681 ctccacttca gtaaatccac aaataaatga attcatattc caagtaccat tcccttcaac
    22741 tacaactttt ctcctgtcct ctttcggata ttcaagaatc agaccgttta ccaatatgtt
    22801 tttcactact actaatttgt ttgtataccg tggaattgct gttccgatat acttcacttt
    22861 atttcctacg ctaattgtca tttcctcact cctattcttt tttatccaaa actttttcct
    22921 gtcctattcc tgccaacgaa tatacttcac tgcatgctta aacaaaacaa aatgttgccc
    22981 tgttttattt ccgtctttta cttgttccac atacagttca tatttggtca caccttttaa
    23041 aacgccagat actttttttc atccattgtc acaattagtt ttttcatcaa tcgattgata
    23101 ctgcactgct catttttctt gttcatatag tattcctttc tttttcaaaa ctagcctctt
    23161 ttgtttacta gttttcatca atcatcaacg atttaacaga ttttttcttt cgcagggttt
    23221 ctagcgtcag caaccccaaa ctctaaaaaa gtgcctttgc tcccttgaaa aacgagcgga
    23281 cgattgtact cgtcatcata tgtttttcat actacctgat ccacgcaaat aacctttttt
    23341 tgttctgtgc cacacccatc catacgtatc gcttgagatt caccatactt ttttaggtgg
    23401 tttatccttc ctaagtccct gttagacacg ctgattaact tagaaagtcc tgtttgtatc
    23461 caattttcaa agatctccca aaagaaataa gtaggtttac gtgtatcgct tctgttcaaa
    23521 gtacctgttt gtacttgagt atttctactc ccctcgttca agggattcct tccaccagct
    23581 agcttttcct ttcatcatca aatgttcctg aaaagcattt acgtttcgtg tccgtgtgct
    23641 tacttattct tttttcgata gcgattatcc gctcgctact cgctatttta tactttgttt
    23701 gttagctcaa ataaaaacta acaaacaaaa aaggctagtt tctttccgtt ttattttttt
    23761 aactgtcgat aaatagttgt tagtttaatt ccaagttcta aaaccaatac gcccaacaaa
    23821 accacaggaa aaacaattgg ggcaaacaag taaaatcgga tagttttcat gtcttccctc
    23881 tttctttttg gtgtcgctgt ggtcgttctt gccgttcaat acttcgatac attttttatt
    23941 cattatttgc tcgttttgtt ttcctctgga ctgcttttct tgctttttga atactttata
    24001 cgcttttcgt gcctgaatta ataggtcttg tcggcgtttc attcgtgaga aaacaatatt
    24061 tgaaaccatc aaatcctggt tcgaacaacc acgattcgca ataattttcc catcaaaaaa
    24121 aatagttttc acttgagaaa aaaacagttg gaaatcttgt tttgttgcta atttttttgt
    24181 caaaatcact gtgcttttcc ctgttgaatc tacaatgatt cttcgaacaa tccgattcga
    24241 taacttccaa cgttgccgat taaacaatgt tacttcacaa caaatatgac gtaagtgatt
    24301 aggggtcatt ttcatgtgtc caattcctcc aaccaattat gcatcgaaat tttgggcaat
    24361 tgtgacagcc cataaatctg cgtttccagg tcttcataac tacaagaacg cttggcttga
    24421 agtaatgaat attctgcttg atctgatagt tttgttgcca ttggatctag attcaattct
    24481 cttacacaac attcccaaaa attcaatagc gctctaaaat aaaatccttt tcgattctga
    24541 attgcttttc cttcatgacg agcaattttt tcattttgaa tacaccgttg atacgttttg
    24601 cttaaatctt ctgcaaatgt ttcccctaaa attcgatact cgcttccatc agataaacca
    24661 tgtgtttgat tatactttcg ttgccgcttt tctataattc gtttactgtc gtatatcatg
    24721 tttagctgct ctttcgcttc acgccattca gctgaactgc gaacaatcca tgtcaaaaca
    24781 tctttcggaa caaacgtact ttcgctgtaa tgagcaataa actctttttg ccaatcatca
    24841 gccatttcaa caggactagt cgtatcgatt tttgtatcta gattttctgt atctagattg
    24901 ttagaagaat tattaaataa ttgtgcagaa ttttcacatg gatttttttc gtcaaaatca
    24961 tcgaaaatcg ctaaattatc aacagattcc tcctcttctt ttccttgtgc agaatctaca
    25021 ctggttgagt caacaggacc taatgaatac acatcatttt ctgtcgtatc taattctcct
    25081 agatacaacc gagctttgtt tctttttcct gtttgttgat caaatcccat atcctctatt
    25141 tctagtagct catattcttt caattctttt ttgattgtat taacagaacg tctgcaacaa
    25201 ttcaatgcat tggctaattc ttgttccgta aattcaaaat aaacaaaacc ttgactatct
    25261 acttgatttc gttgaatggc taaatctaag cgtgaccgaa aaaccatata cgcgattttt
    25321 gctttatcag acaatgagcg atatttttca cttgtaataa atacctttgg aaattgatag
    25381 tataattcat tgtatttacg agttgcggta aatctttttg ttgccatcgt tattacacct
    25441 catcttgtcc atatattaag aaagaatttt ggtgttggta gacacgaaca tcttccattg
    25501 tagcaatcaa ttcttttaga taaattcgac cgaattcaat tgttagtaag tcgtaatctt
    25561 ctaattcatt tttgatttct tcaatttctt cttcagacac attgagtaaa atggctaatt
    25621 cttgattggt ataagaaata tagacatgtt tcttttcatc aatccgtttt tcaattaagg
    25681 attcttctaa gtactcattc agtaagctat aggcgtattt tgcttgtgca gtcatcgcat
    25741 aatattttga cgattttata aagaatagtt ttggcatatg aaaagttgct tgtgtcagca
    25801 catctgttgc gcgaaatttg ttttttacca tattttttcc tcctagaaaa acacgcaaaa
    25861 aaagctctgt ataaatcgaa ttacacagag tttcttcttg ttgatattta atttctaaaa
    25921 tacgtttcgg gtaaaaacta aacacttact aatatttaga ttgtagaatc ttcgccttca
    25981 tttattcatt ttaaagagaa aaagatcaag tgaaatgtgc ccaaaagttt gattctatta
    26041 atttttaaac accattcata atatatttcc tataatgtaa aaagcgtcga tacttattaa
    26101 agcatcgacg ttatttttat agtaaattta tcaaaatttt tgccaacatt ttttccgatt
    26161 tttttcccta tttctatctt tccttatctt tcttagttaa tattatttga tgttaatata
    26221 tcactactaa tattaaaaaa cattgattta tcataggata aagagtgata gtttaatact
    26281 taaagagaca tattaatttt atttaaaaat tgacattaat gactcttaat cagcgggtcg
    26341 cgggttcgag cccctcacgg cccattgggt gccaaaccca cggaacaggt aaatgttggt
    26401 gcgaaagcat ttcgaaaaca tttacgctgt tcttttttta
//
LOCUS       DAMYXM010000032        25523 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.033, whole genome shotgun sequence.
ACCESSION   DAMYXM010000032 DAMYXM010000000
VERSION     DAMYXM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.033"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            132..3659
                     /locus_tag="PNS21_001551"
     CDS             132..3659
                     /locus_tag="PNS21_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDI5159263.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3656..7378
                     /gene="addA"
                     /locus_tag="PNS21_001552"
     CDS             3656..7378
                     /gene="addA"
                     /locus_tag="PNS21_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDI5159264.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7447..7788
                     /locus_tag="PNS21_001553"
     CDS             7447..7788
                     /locus_tag="PNS21_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI5159265.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8097..9140
                     /gene="pheS"
                     /locus_tag="PNS21_001554"
     CDS             8097..9140
                     /gene="pheS"
                     /locus_tag="PNS21_001554"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDI5159266.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9145..11565
                     /locus_tag="PNS21_001555"
     CDS             9145..11565
                     /locus_tag="PNS21_001555"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDI5159267.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="PNS21_001556"
     CDS             complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="PNS21_001556"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDI5159268.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12999..13736
                     /locus_tag="PNS21_001557"
     CDS             12999..13736
                     /locus_tag="PNS21_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDI5159269.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13750..14565
                     /locus_tag="PNS21_001558"
     CDS             13750..14565
                     /locus_tag="PNS21_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDI5159270.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14578..15225
                     /locus_tag="PNS21_001559"
     CDS             14578..15225
                     /locus_tag="PNS21_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDI5159271.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15241..15897
                     /locus_tag="PNS21_001560"
     CDS             15241..15897
                     /locus_tag="PNS21_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDI5159272.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16059..16880
                     /gene="racE"
                     /locus_tag="PNS21_001561"
     CDS             16059..16880
                     /gene="racE"
                     /locus_tag="PNS21_001561"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDI5159273.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16883..18238
                     /gene="rph"
                     /locus_tag="PNS21_001562"
     CDS             16883..18238
                     /gene="rph"
                     /locus_tag="PNS21_001562"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDI5159274.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18239..18757
                     /locus_tag="PNS21_001563"
     CDS             18239..18757
                     /locus_tag="PNS21_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDI5159275.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18864..19364
                     /locus_tag="PNS21_001564"
     CDS             18864..19364
                     /locus_tag="PNS21_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDI5159276.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19644..20564)
                     /locus_tag="PNS21_001565"
     CDS             complement(19644..20564)
                     /locus_tag="PNS21_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDI5159277.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20729..21742
                     /locus_tag="PNS21_001566"
     CDS             20729..21742
                     /locus_tag="PNS21_001566"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDI5159278.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21852..22607)
                     /gene="fabI"
                     /locus_tag="PNS21_001567"
     CDS             complement(21852..22607)
                     /gene="fabI"
                     /locus_tag="PNS21_001567"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDI5159279.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22827..23267
                     /gene="fabZ"
                     /locus_tag="PNS21_001568"
     CDS             22827..23267
                     /gene="fabZ"
                     /locus_tag="PNS21_001568"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDI5159280.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23382..24143)
                     /locus_tag="PNS21_001569"
     CDS             complement(23382..24143)
                     /locus_tag="PNS21_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDI5159281.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24332..25426
                     /locus_tag="PNS21_001570"
     CDS             24332..25426
                     /locus_tag="PNS21_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDI5159282.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8455 a         4426 c         5387 g         7255 t
ORIGIN      
        1 ttttgcgtat acttaaaaaa gtagaaatat cagaacctta aaaggatata atttgaagaa
       61 tatagaacaa atgttcagta ttttttgata taataaagga gcaccgtatc atgtgttgaa
      121 ggaggaaaag catgtcttta caatttattc ttggaaacgg gaaaaaagat caccgtcaag
      181 tcttgatcga cgaagcatcc gcttggctcg accaggatgc aagtaaccaa gtatttttcc
      241 ttgttcccaa ctacagcaaa ttcgaacagg aacaagaaat tttagcggag atgcgaagaa
      301 gaagcggaaa aaaaactttc agcacaacaa acgttcaagt atttagtttt tatcgtctgg
      361 cttggtattt gctgcaacag acaactcttc ttacaggaaa tgaattatca gaatcaggat
      421 ctgcaatgat cttgcgacag attttagaaa aaaatgagga agaattaaca atctttcgcg
      481 gagaaatcaa taaaagaggg tttattcgtc aattacaaga actctacagt gaattgcgta
      541 caggaaatat tcaaccagaa gacctgatgc ttttatttgg tgattcacct aatgcagaag
      601 accagcaact taaaatgaaa gacttgaagc tgattttttc agcttatgac agcgaattga
      661 ctcaacgtgc actaacaaat gaagatgcac tatctatact agcaaattat atgcagacac
      721 aagatttcaa caacgtcaaa tttattgtca gtggtttttc tcgaatcaat ggtcaagagt
      781 atcaattact gcaggcaatg atggaaaagg ggcaattggt tgtcgattta ttgctggatc
      841 gtccttatac agcggatctt ccagaagtac ttacgctctt tcatgagact ggaaagcttt
      901 atttccgtct ttttcaaagt gcaagagaac ggaaagtccc tatattagtg gaccgatttg
      961 caccagagca gactcgtccg acagagcttc aactgctgca aacaatgtgg gaagagacat
     1021 ctagccaaaa gaaagcggca gtacacaaaa tagggggaca agagcatctc catttgtggg
     1081 aagctgaaaa tccaactgaa gaagtccgac aattagctgt tgaaatcaga cgactagtca
     1141 gtgaagaaaa ctaccgttat cgtgatatcc aagtgctgac aaaaaatatg tctttatatg
     1201 gaaatatcct tcggccgatc tttcaagaaa tggcaattcc tttttatatc gatgaagaac
     1261 aaatgatgga aaatcatccg ctgatcgaat ggatcaacag tctgttcgct cttgaccgct
     1321 attcttatcg gctaagtgat attatgcgtt ttttcaaaac tgaattattt ctgtcgacgg
     1381 aagcagaaac cgaacttgat gaatggaaac aagagcgaga tgaattcagg aaaaaactgg
     1441 acatcactga aaatgtagca cttgcttaca attatcaagg aacttattgg actcgtgaaa
     1501 aagactggca gtttgtggcg tatgattttg aagcagaaca gattgaagac gtacagtatt
     1561 tggaagaaca gtcaaatacg attagacgat tatttcagaa agctgtacca gtatttttga
     1621 aaaaaataaa gaaaagcaaa acaggacaag aggccgcaaa tgttttttat caatttttga
     1681 tccaaagtgg cgtacagcaa caattattgt tttggcgtaa tcaggaagta gacagaggaa
     1741 acttagaagc ggcaagaaac catgagcaga catggggagc gctgattgat ctgttggatg
     1801 aatatgtagt aatttatggt gaatcggctt ttgactggtc actgttccaa gaaatcatta
     1861 caagtggtct agaaaatcta agttatggta aaatccctac cgcaatcgac caagtgcgaa
     1921 tcaatcgttt ggaattagtt cgaccaaatc aagctgccgt cacttttgca ttagggttga
     1981 atgatcaagt gtttccagac cgaaaagaga caaaaggatt gttgtccagt gaagaacgag
     2041 aagtattaaa tcaggccttg ccagatggac agtttttatt cgacccatca aaggaaagta
     2101 tctcttttga gcctttccaa gcctatcttg tttttctttc cggaacagat cgattatatc
     2161 ttagttatgc acaaagctat gacacagatg gttcgctgaa gatgtctcct tatttgcgta
     2221 gaatcacaga atatttatct gttcccgtcg agagaaaaga gcatttgacg atcgaaagcg
     2281 accctaattg ttatgtaggg acttacagaa gcggaatcaa tacagtcaat aggatttatc
     2341 gattagcttt agatgaaaaa aaacctgtac caaagcactg gcaaaagctc aaagaattag
     2401 tactgaattc gtcatggcga aattttgctt tacagatttt tgaaagtcag gaacatttaa
     2461 atgttcctgt ttcgatgcca gaaaaaatgg cggaagagct ttatggaaaa gagatttata
     2521 cttcaatttc acgtatggag aatttttata attgtgaata caaatacttc atgcaatttg
     2581 gtttgaaatt gaaagaacga actgtttatg gtctaactcc agcagcaact ggtgattttt
     2641 atcatgaatc attggatcgt ttcttcaagc tactgttttc aaaccaatta tccttagtga
     2701 atatgtccga tgcgcagcgt agagacttta ctgaaaaagt gctgcaggaa gtattcggag
     2761 aacttcggtt tgagatattg gatagttctg caagaatgaa ctatatccgc taccagttag
     2821 gacagacgat tcaaaaagtg acatgggctt tgcaaaaaca gagcaaaaat acaggcatgc
     2881 atcctcttca gacagagatt ctatttggtc agattgctgg agcaaaaggt atcacaggtc
     2941 tggaattacc gttgagtaat ggtggaaaac tccatgttcg agggaaaatc gatcgaatcg
     3001 atgtagcttc tgaacaagaa gatacttggc taagcgtcgt tgattacaaa tctagcggac
     3061 gttcattcga tgtcacagaa gcttattatg gcatggctat gcaattattg acgtatctgg
     3121 acgtagcatt gatggatgcg gtacgtttga ctggaaaggc ggctgttaaa ccagcaggat
     3181 catattattt acatgttcat aatcctgtat tgacggagac agatgatgta gagaagaata
     3241 cattgaaaaa atttagttat gatggcgttt ttgtcgatga tcccgctctt ttagaagtac
     3301 tagatcattc actggaagca aaagaacaat cactgatcta tccagtgaaa aaaaatgcga
     3361 aagatgaata ccaaaaaggt agtttcagta aagacaaatt tttcacggaa gaagaactgc
     3421 aactattcat ggcgcataat cgggaaaata tgcggttagc tggagatgcg attacttccg
     3481 gagaaatcaa actaaatcca gcttataaag ataaacagcg gattgcctgc gaattttgtc
     3541 cattcagaag catctgtacg tttgatgtga tgctgaaaga aaataattac caccgtctgg
     3601 agaaaataga taaggaagaa gcactgcgta gaatattgaa aaggagtgaa gaagagtgaa
     3661 tcaaattcct ttaaaaccgc aaaatgaacg attcacagat gatcagtggc aagcgatttt
     3721 tgatcaagga gataatctat tagtctctgc gtctgccgga tcaggtaaaa ctacagtgct
     3781 tgttcgaaga gtgattgaaa aactaaaaat gggttttgat atcgatgaat tattgattgt
     3841 gacatttact gaagctgccg caagagaaat gaaagaaaga attcaagagg cattacaaga
     3901 atccgtcaat agcgaaagtg accctgtacg tagacagcat tttactaagc agttagtttt
     3961 attgcccaca gcgaatatct caaccttaca tgctttttgc ctgacagtta ttcgacgtta
     4021 ttattactta attgatattg atccagtctt tcgcatgtta acagatgaaa cagaaacgat
     4081 cttgatgaag gaggatgtgt gggacgagct gagagaagca ctttatgcag aaaatgatga
     4141 acgatttttc caattaacaa tgaatttttc taatgatcgt tcagatgacg gcttgacgaa
     4201 tctggtgttt tctctttatg agtttgcccg tgccaaccct gacccccaaa aatggctaga
     4261 acagttaagc gataattatc gtttgcctga ggggctagcg aaatctcgat tatatcaaaa
     4321 acaaatccgt cctttagttt tggcagatat ataccaatgt gtacagttat atgaacagat
     4381 gactcagtta gcacaaggtg aaggattaga aaaaatgcat gagcaagtag ctggagaaca
     4441 acagcagatc aagaatatct atgaagcgtt ctcgcaagat cggttagaag aagcttatgc
     4501 tggtttggaa caactaactt tttcaacttt taagagcagt cggaaagcag aattgaaaga
     4561 aatttccaat gaagtcaagg gcatgaggga taaagcaaaa aaactgattc agcagataag
     4621 taaaagttat tttcctgttt ccccaagcca aatggaagaa ttaacggaca aagcactacc
     4681 gttagtcgaa gaaatgacaa aagtcacgca aagcttcatg gacggtttca gtatgcgtaa
     4741 acgggaaaaa ggtgtgctgg atttcaatga tctggaacat ttagcgttgc agattctcac
     4801 agaaaaaacg aaagatgtat ggctgccaag tgaagcttca aaacattatc gaaaaaaatt
     4861 caaagaagta atggtagatg aatatcaaga cgtgaaccaa ttacaagaag ccattttata
     4921 ctggctaaga gaacccgatg atacaaaagg aaatatgttc atggttgggg atgtcaaaca
     4981 atccatctat tctttccgtt tagcagatcc tagtttattt atcgggaaat acgagaattt
     5041 ctcaaagaaa gaaggcggac gtcgaatcgt tttggctgag aacttccgct ctagaaaaga
     5101 agtcctttct tttacgaatc ttatttttga acaactaatg gatccagcag tgggtcaaat
     5161 caattatgac gaagcagcca aactgatcca aggattttca gactttccag agaatgaaca
     5221 gtttgagccg gaaatcatga tctatgaaaa agaacaggaa gaatcagaga ttgagattcc
     5281 gacggatgat atactggaag acaaaacaga aggcgaactt tttatgacag gactaaaaat
     5341 tcgtcaatta attgattcct cctttatgat ttatgataaa aaatcaaaaa aaagtcgtcc
     5401 gatagagtac aaagacattg ttctcttgac acctacaaag aagaacaact taacgatttt
     5461 agagattttc aaaacattgg atattccatt agagatgaat gatgctcaaa actattttca
     5521 agcgacagaa attcgcacga tgatctcgct gctacaatta atcgataatc cctatcaaga
     5581 tattccgctt gcggctgtat tgcgctcacc gatcgtgggt ctgattgaac cagaattagc
     5641 cagcattcga ttagctaacc gtgcacatac ttactatgat gctgtgcttg cttatcaagc
     5701 atcaaacgaa gatgaattag cagctaagct ggaacatttt ggcaagcaac tagagcattg
     5761 gcgagaactt gctcgccgtt cttcgatcac tgacttgtta tgggatatct attatgagac
     5821 gggatatcta gagtatgtgg tcggtctgcc agcaggggca cagagacaag ccaatcttta
     5881 tgcattggtt gatcgtgcca aagcttacga gcaaagttct ttcagaggat tataccagtt
     5941 cgtgcgtttc attgaaaaaa tgcaagaaaa agataaagac ttagcagaac cagtcatttc
     6001 aatagaagat aatgctgttc gcgttatgac gatccatgca agtaaaggct tggaattccc
     6061 agttgtattt ttgttagata tgacaaagga gttcaactta caagacttgc gcaatcggta
     6121 tgcttttgaa gaaaaacttg gagcaggaat tcgctatatg gatcctgaaa cacgagtgtt
     6181 atatgatacg ttaccgtttc aagccatcaa attggcaaag caaaacaaat tgttgtcgga
     6241 agaaatgcgt aaattgtatg taggattgac tcgggcagaa caaaagcttt tcattgtcgg
     6301 ttcctataaa aataaagagc aaatgatcca aacgtggtct gaagcagctg atcatgagga
     6361 gttagttttt gatcctgcgc tacgtttgaa gggcagaagc agcttgatga attggattgg
     6421 ttacggattg atccgtcatc ctgaaatgca gaaatactta gaggaagaaa tttcgacttc
     6481 tcttcttcaa cattcaaatg cacagttttc tatttcatgg atgaatcagc aatcgatcat
     6541 tgaacaaaga cagttgcttg ctgaaaaaga actggttaac ttagatcagc agatgaaaga
     6601 agatgaaact ttattagcag attcgttaca aaaacgtctg gcatatgaat atccttatca
     6661 agcttcttca caaaccacaa gttatcaatc tgtatctgaa atcaaacgat tattcgaaga
     6721 tccagatgat acacaagaat cacgtttgac tctggaaagc agtcagaaca aagcagcatc
     6781 ccgtcaattt cgttatacgc aagaacaact agcagaacca aaatttttgc aaaaagatcg
     6841 gcaagtgtca gcagctgctg ttggaactgc tacgcatgct ttgttacaat tactgccatt
     6901 agagatgcca actacagaga gtatccatca aaaattacag gaattggtaa ggaaacggtt
     6961 agtagacgaa aaagtcgcga aaaaagtaga tgtttcttct attatatggt tctttcaaac
     7021 agaacttggt caacagctga ttgccaacaa agaaaacgta aaacgagaac aacctttttc
     7081 catgctgctt cctgctgacg aagtatttca agactatcca aatcaagaag atgaactatt
     7141 aatccacgga atcgtcgatg gatacctaga agaaaaagat catctcaata tctatgattt
     7201 taaaacagat tttattttac atccggatga tccagcagaa atagatgcaa tcgtccaaaa
     7261 atatcaagga cagctaagac tttaccaaca agcaatgtca gaagctttga ataagccagt
     7321 agaaaatgtt ttcctaattt tgcttcgagt gaaacaaatt ataaacatta acaaataatt
     7381 ataattgagc aaacttgact tatgttttgt aaggattatg ctatactctg ggaagaggtg
     7441 agtatgatgg agctaacaca gacagaattc gttttatgcc ctaaatttga aaaaacattc
     7501 tctattttag gaaaaaaatg gaacggattg attatcgatg tactactcga aaacggtccg
     7561 caacgctttg ttgagcttgc ggcaaaaatc ccgatggtca gtgatcgagt attagtcgaa
     7621 cgcttgaagg aattagaaaa agaaggaata gtcactcgaa actttgcctg tagcgaaaag
     7681 agccgttcag aatatctctt gactgaaaaa ggagcagact tgcaaaaagc gatgcgtcaa
     7741 atacaagttt ggagcgaaaa atggatcaca gaggaagaat gcggttgatt ttgtaaaaaa
     7801 aatcttgtaa actatagtcc atacgaataa gtaaagaacg atgacgaaaa agagtagttt
     7861 ggctaaacca gttcagggag atctgtcgca gactgaaagc agatcctgtg tttgaaaaat
     7921 gaagttcact ttccgagttc gtctgataaa atcagaccgg caattgtcgt taacgaagta
     7981 agagtgcggt gtaagtcatt tatccgcaaa ttaggatggt aacgcgaaga cctcgttcct
     8041 taacaggaat gaggtctttt tgttttgtat ttggaattta aaggaggaac aagcacatgt
     8101 ctctacaagc acaattagag agcttgaaag aagcaacgct cgtcaaaatc acagcggcac
     8161 aagatttaaa tgaattaaat caaatacgtg ttgaaacgtt aggaaaaaaa ggaccgatca
     8221 cagaagtatt acgtgggatg aaagatctaa gtccagaaga gcgaccaaaa gtcggaagtt
     8281 tcgcaaatga gatccgagat cttttgacag aagagatcga gcgaaaaaaa caagtattag
     8341 aagaagaagc gttaaatgca gctttggcaa aagaaacaat agacgtgaca ctacctggta
     8401 gaaaagtaaa acatggtaca cgtcatatcg tgacacaaat catggaagaa attgaagata
     8461 tttttgtggg tatgggctat cagatcgtag aaggatatga agtagaatct gatcattaca
     8521 atttcgaacg gatgaatctg ccaaaagatc atccagcaag ggacatgcaa gatactttct
     8581 atatttcaga cgagatattg attcggacac atacttcacc agtccaagca cggacaatgg
     8641 aaaaacatga tttctctaag ggtgctctac gaatgatctc gccagggaaa gtcttccgca
     8701 gagatacaga tgatgcgacc cacagtcatc agttccatca aattgaaggg ctagttgttg
     8761 ataaaaacat cacaatgggc gaccttaaag gaacattaga agtcgtaatg aaaaaaatgt
     8821 ttggggaaga tcgtgaaatt cgtttgcgtc caagttattt cccatttacg gaaccatcag
     8881 tagaagtaga tgtcagctgt ttcaaatgtg gtggtgccgg ttgtaacgta tgtaaataca
     8941 ctggatggat cgaaatttta ggagctggca tggtacaccc gaatgtgttg aagatgtcag
     9001 gaatcgatcc agaagaatat tcaggctttg ctttcggcct tggaccagac cgcgtagcta
     9061 tgttgcggta tggagtgaat gatatccgta atttctatca aaatgactta cgtttcttaa
     9121 gtcagttcaa gggggagtaa gaaaatgtta gtttcatata aatggctcaa tcgttatgta
     9181 gatttatcga atgtaacacc aaaagaatta gcggataaga tgtctgtgac aggaatcgaa
     9241 gtagagggag taacggttcc agaagaaggc ttgaaaaaaa tcgttgtcgg tgaagtcaag
     9301 gaatgcgtac cacatccaga ttctgatcac ctttctattt gtcaagtcga tattggcgaa
     9361 gacgaactgt ctcaaatcgt ttgtggagcg cctaatgtga aggttggaat caaagtgatt
     9421 gttgcacttc ctaactcacg tatcgctggt aatgtaaaaa tcaaaaaagg aaaaatgcgt
     9481 ggccaagtat caaatggcat gatttgctca ttacaagaaa ttggctattc agatagtgtt
     9541 ataccgaaag aatacgctga aggtatctat tatatgccac aagaagctgt aaatggcgat
     9601 ccagtttttc cttatttgga tatggatgat tcgattattg aattatctat cacaccgaat
     9661 cgtgcggatg cattgtcaat gcgtggcgta gcttatgaag ttggggcaat ttatcgtcag
     9721 actcctacat tcgatgacga aaagctggta gaatcctcac gtcctgctag cgataaaatt
     9781 caagtggaag ttgaagataa agaagcagtt cctgcttatc aaatcagaat cattgaagga
     9841 gtaagaatcc agccaagtcc tcaatggctg caaaatcttt tgatgaacga aggcattcgt
     9901 ccaatcaaca atgtagtaga tgtgacgaac tatattttgc ttttattcgg acaaccacta
     9961 catgcatttg actacgacaa attgaaatca aaagagatct ttgtccgccg tgcaaaagaa
    10021 aatgaaacat tagtaacact agatggagaa tctcgggaat tgacaactga caatctcgtc
    10081 attactaacg gcaaagaacc agttgcttta gctggtgtca tgggagggtt agactcagag
    10141 atcacgaatg aaacaacaac agttgcacta gaatctgctt tatttgatcc aatttcgatt
    10201 cgtcgtacag caaaacaatt taatttgcgt agtgaatcct ctgcacgttt tgaaaaagga
    10261 atcaaccatg cgactgttgg agaagccagt gaggttgctg cagcaatgat cgtttctctt
    10321 gcgggtgggg aagttctaca aggcgcggtc aaaggctcag agatcaaagc agaagacgta
    10381 tcagtgaaga tttcacttga acgcatcaat caatatttag gaacatctct tgtcgttaaa
    10441 gaagtaaatg agatttttga agcacttgga tttagctatg aagaaaagga gtatgtttat
    10501 tccgtagtag taccaccaag acgctgggat atccaaatcg aagcagatat tatcgaagaa
    10561 gttgctcgta tttatggata cgaccagttg ccatctacac ttccaagtgg agaaacggtt
    10621 gctggaagct tgtcaaaaga acaagaaact actcgtaaaa ttcgtacaat ctttgaagga
    10681 agcggattga gcgaagcaat cagttatgca ttgacaacag aagaaaaatc aagacagttt
    10741 gttataaatg aaagtaatat tacacgtttg gactggccaa tgagcgaaga acgttctgta
    10801 ttacgaatga atttgatcag cggactatta gatgatatca gttataacac tgcccgaaaa
    10861 aatacagaga ttgctttata tgaaattggt cgggtgttct tccaagaaaa tgatccacgt
    10921 acacatctgc cgcaagaagt aaaacatgca gcaatcgcta tttctggtat ttgggaagaa
    10981 aaagattggc agacgaaagc tcaaaatgta gactttttca ccatcaaggg tcttttagaa
    11041 aacttatttg aacaactagg catcactgaa gatgtccatt atcaaacagt gacagaaatg
    11101 aaggaaatgc atcctggaag aactgctgcg atttaccttg gcgaaaaatt tattggtttt
    11161 gtcggtcaag tgcatccaac gattacaaaa agttatgata ttccagaaac atatgttgct
    11221 gagtttgatt tatctgctat agtggaagct gctcaaaaag ggatcgtttt cgaagcagtg
    11281 acaaaattcc cagcagtcag ccgggacatc gctttgcttg taaaagaaac tgtgacaaat
    11341 caggaattaa cagaagttat ccgttctgct gccggaagat ttttaacaga tatccaatta
    11401 tttgatgtct atcaaggaga aaacattgaa aaaggacata aatccatggc gtatagcctg
    11461 acattcgtca acccagaagc aacattgaca gatgaagaaa tcaataaagc aatggaaaaa
    11521 gtcgaaaaag cgttggtaga aaacctagag gcaagtattc gctaagaagg aaaatatagt
    11581 aagtaacgat caagtagctt ttgaagacca tacatttgaa aaaaagatgt gacacaactt
    11641 cgtcacatct ctttttttga ttttaaaaaa ggaatagcac agagacgaga taaaaaaaca
    11701 ggcctttaca gcctgctttt ttagagtaaa tgaattaatg gaagtaattg attcccattg
    11761 ctgattttac ttcagaaagt gtttgtgcag ctactttagc agcttcttca ctacctttac
    11821 gtaagatttc cataacagct gaaggatctt tttccaattc ttctcggcgc gcacggatcg
    11881 gtgcaaattc agcttccaat acatcaatga ggtatcgttt gatctttaca tcaccaagtc
    11941 cgccgcggcg gtaatgagct ttcatttctt caattgcttc tttatctgtt ccgaatacat
    12001 ccaagtaggt gaagaccata ttcccttcta cttgtccagg atcttgtaca tggatgtggt
    12061 ttggatcagt atacatactc atgacttttt tctgtaagac atcggcagga tcggagatat
    12121 aaataccgtt tcctaacgat ttactcattt tgccatttcc atcgattcca ggtaatctac
    12181 ccattccttt tggtgggaat acgccttttg gttccaccaa gacttcacca tatgtatgat
    12241 tgaagctttg tacgatttcc tgtgtctgct ctagcattgg tttttgatcc tcaccaacag
    12301 gtacaagatt cgctttgaat gcagtgatat ctgctgcttg agaaacagga tagataaaga
    12361 atcctgtagg cacgctttca ccgaattttt tttgttcgat ttctgttttg acagtcggat
    12421 tgcgccgaac gcgtcctacg ctgactaagt tcaaataata catggttaat tcagctaatt
    12481 ccggaatttg tgattggata aataaagtag attttgcagg atctaatccc acggctaaat
    12541 agtctaaagc aacttgcaag acatttgatg aaaccttctc aggattttta gcattatctg
    12601 tcagtgcttg catgtcagca atcatgacaa acagttggtt gtcttcatct gcctgcatag
    12661 ccactcgggt ttttagagaa ccgacgtaat gccctaaatg cagcttccca gttggtcgat
    12721 ctcccgttaa aataatattt ttcaaaaaag acacctcttt acgatttgta agtctgagca
    12781 cttaatatct taccgttttt cctgaaaaga aacaacagtt cctaaggaaa aaacaaggaa
    12841 agaggataaa ataactaata gattagaaat ggaagaaata tttatgggag aataaacgtt
    12901 ctaagaaact ttacaaatta ctaaactatc agatataatg tattttatta tacatcttta
    12961 aaagaaatct tactaaataa taatgggagt gtaattctat ggcaatgatt aaatttgatc
    13021 acgtggataa atattatggc aagttccacg cattaaaaaa tatcaatctc gaattcgaga
    13081 aaggagaagt tgtcgttgtc attggtcctt ctggttcagg aaagagcacg atgcttcgct
    13141 gtatcaatgg attagagacg atctcttcag gtaagctgct gattaatgat actgacttgc
    13201 atgataaaaa gacaaaactg acagaagtcc gaaaaaacat cggcatggtt ttccaacatt
    13261 tcaatttgta cccaaataaa actgttctgg aaaacatcaa attagcacca atcaaagtgt
    13321 tgaagcaaga ccaggcaact gcagtcaaaa acgcggagaa atttttgaaa actgtcaata
    13381 tgttagataa gaaagattct tatccttcca tgttatccgg gggacaacag caacgggtgg
    13441 caatcgccag aggtttagcg atgaatcctg agatgctgct gtttgatgaa cctacttctg
    13501 ctttggatcc ggaaatgatt ggtgatgtat tagacgttat gaaaaaattg gctcatgacg
    13561 gtatgtcaat gattgtcgtg acacatgaaa tgggctttgc caaagaagtg gcggatcgtg
    13621 ttatcttcat ggctgatgga gaagtattgg aagacagcag aaatgtgaga gactttttcg
    13681 aaaatccaaa tgaagtccga gcacagcaat ttatcagtaa agttatcaat cattgatagg
    13741 aggaaaaaga tgcgcaggtt aaaaaaaata cgcctgattt tccttttagg cattgctttt
    13801 cttgcactag ccggatgtaa aggaaatggg ttggcaaatg aagatatttt agagcgaagc
    13861 aaatctacca atgaaatcat ctggggtgtc aagtatgata ctcgattatt tggaatgatg
    13921 gatattgaga gcagaactgt acaaggattt gatgtagata ttgcaaaagc aatcactaaa
    13981 aagatccttg gtgacaatgg aaaaacagaa tttgttgaag tcacttctaa aactcggatt
    14041 ccattattga aaaacggaaa tatcgatgca attattgcaa cgatgacgat cacagatgaa
    14101 cgaaaaaaac aggttgactt ttcagatgtc tatttcgatg ctggccaagc tttgttggta
    14161 aagaaaggaa gccaaatcaa aagtgtagat gatttgaatg cttctacaac ggttctagca
    14221 gtcaaaggtt ctacatcggc tgcgaatatc cgacagcatg caccagatgc aaaaatttta
    14281 gaattagaaa actatgcaga agcatttact gctttacaat ctggtcaggg agacgcgatg
    14341 actacggata atgcgatttt gttagggatt gcagatgaaa atccagagta tgagttagtt
    14401 ggtggaacgt ttactaatga gccttacggg attgcgatca acaaagggca agaaaacttc
    14461 ttgaaagctg ttaaccaagc gctagaggaa atgcatgcag atggcacata cgataaaatc
    14521 tatcaaaaat ggtttccaaa tgaaactgaa ggaaaagtag agtaaggggg agaggcaatg
    14581 agcgaattga tacaaactta tggaccaatc tttgtcgacg ggttcaagat cacattgttt
    14641 tcgagtgtct tagcacttgt gtttagtttg attatcggaa cgttaatggc aatcttccaa
    14701 ttatcaaaga ataaagtagt gagcgcctta gcaaaggcat atgtagaatt ttttagaaac
    14761 attccattat taatcatcgt gatgtttttt tatgttgtcg taccgattta ttggtttaat
    14821 ctagatggat tccaagcagg tacgattgga ttaacgatct atacatctgc ttttattgct
    14881 gaaacagtac gttcagggat tcaaaccgta ccaaaaggtc aaacagaagc aggactatct
    14941 tctggtttta cctattcaga aatcatgcgt tatattgttt taccgcaagc attcaaaatc
    15001 gttatcccgc cattaggcaa ccaatttatc aacctcgtaa aaaactcatc tattttagca
    15061 atcgtagctg gtttagattt gatgtatcaa ggagacttga tcgctagtga aacattcaat
    15121 accttcgata cgtatatcat cgttgggtta ttttacttag ttatcacgct accgttatct
    15181 tacttaatgg tttatcttga gaaaaaatgg gcggttcgtt catagaagga ggggaaatac
    15241 atggatttta gcggcgcttt ttcatggatg aatatccgtt ttttattgga aggattaaag
    15301 gttactgttg aagtttcatt gttttcgatc atttttagtt tcttgatcgg tggtctgaca
    15361 ggtgtgattc gttttgcgaa tatccctttc ttttccaaaa tccttggtgt agtgattgat
    15421 attatacgga atcttccatt attattgatc attttcttca cttactttgc cttacctcaa
    15481 atcggcattc agatgaacat cttctggtcc gctgtcgcag cattaacgat ttttgagtca
    15541 gctatgttgt cggaaatttt ccgagctgga ctcaatgctg ttccaaaagg acagatggaa
    15601 gcaggattat caacgggact gacttatgta gaatcgatgc gaacgatcgt gttaccgcaa
    15661 gcatttaaat caatgatccc ggcaattgtg agccaattga tttcactgat caaagatact
    15721 tcattagcag taatcatttc tttacctgaa ctttctcatc aggcacgtat tatttatgga
    15781 cagaacacca attatgtgct tccgatgttc atcatcatga cacttatgta cttcgtggta
    15841 tgttacgcgc tttctctgct ttccggttac ttggaaaaga gacggtacag ttattaatag
    15901 atgattcttt tagcggttgt ggcaaactaa agtcacaacc gcttttcctc tttccattcg
    15961 aaaaaaagag atttcgattg ttttaagcaa gtaagagcac ttgaaaattc acgctaaaaa
    16021 gtacacaata gggaggatag aatttattga aaaaaaggat gatacgattg acagataatc
    16081 gccctatcgg atttattgat tcaggtgtcg gcggcttgac tgtagtaaaa gaagccctga
    16141 aacaattacc gaatgaaaat attttatttg taggagacac agcacgctgc ccatatggcc
    16201 ctagacccgc ggaacaggta atccagtata cttgggaaat gacggattat ctggtggagc
    16261 aaggaatcaa gatgctggtg atcgcctgca ataccgcaac tgcggtggct ttagaagaaa
    16321 tcaaagctgc tctttctatt ccagtcatcg gtgtgatcct tcccggtact agagcggcag
    16381 taaaaaaaac acaaaataaa caagttggca ttatcggtac gattggtacg gtaaaaagtc
    16441 aagcttatga aaaagcactg aaagagaaag taccagaatt gactgtgaca agtcttgctt
    16501 gtcccaaatt tgtttcagtt gtcgaaagta atgaatacca ttcatcggtg gcgaaaaaaa
    16561 ttgtggcaga aacattagct cctttaacca ctaaaaaaat cgatacattg attttgggat
    16621 gcacccatta tccattatta cgccccatca ttcaaaatgt aatgggagaa aatgttcaac
    16681 tgatcgattc tggagcagaa acagtaggtg aagtatctat gctgttagat tatttcaatt
    16741 tgagcaattc accgcaaaat ggtcggacat tatgccagtt ttatacaact ggctctgcca
    16801 aacttttcga ggaaatagct gaagactggc ttggaatcgg acacttaaat gtagaacata
    16861 tcgaattggg aggaaaataa ggatgcgtca tgacgggaga aaagcccaag agcttcgcaa
    16921 actggagctt caaacaaatg ttttcaaata tccagaagga tcggtagtga tccgttttgg
    16981 tgatacaaca gttatttgtt ctgcaacaat agaaaattct gttccccctt ttttgagaga
    17041 aacaggaaca ggatgggtga cagcagaata tagtatgctg cctcgagcaa cgagtacgag
    17101 aaatcgtaga gaaagcagca aagggaaact ttctggtaga acaatggaaa tccagcgtct
    17161 tattggtcgt tccttgcgtg cagtagtaga cttggaaaaa ttaggagaac gtagtatcat
    17221 cgtagactgc gatgtgatcc aagcggatgg cggtactaga acagcaagta tcactggggc
    17281 atttgtggca ttgaaattag ccattgaaaa acttttaaga gaaaaagaat tgtcagaaga
    17341 tccaataaaa gaacatcttg ctgcagttag cgtaggaatc ttaccagatg ggacttgcgt
    17401 cacagatttg gattaccaag aagattccgc agcacttgta gacatgaatc ttgtaatgac
    17461 agaatctgga aaattcgtgg agattcaagg aactggagaa gaagcaacgt ttgatggtga
    17521 acagttgaat gaaatgctct tttttggaaa aaacgcgatt gaagatttga tcaaagagca
    17581 aaaacatgct ttgctaacag agtttgctca aaatgatgag cggatagaag aaacaaaaac
    17641 aatcatcatt gcgacaagaa atccaggaaa agcagaagag tttcgaaata tgttcaaaga
    17701 agcgggatat catgtaaaaa cattacttga ctatcctgaa ttgccagatg tggaagaaac
    17761 tggcagcaca tttgaagaaa atgcccgatt aaaagcagaa acgattgcac aattgcttga
    17821 tcagccagtc ttagcagatg attcaggttt gaaagtggat gctttaggcg gtatgccagg
    17881 aatctattct gcacgttttg caggggaaca aaaaagtgat gcggggaata acgccaaatt
    17941 attatatgaa ttgacagatg tgccggatga aaaaagaaca gcacaattcc attgtacgtt
    18001 agtattcgcg gcacccaaaa aagacagcct tgtagtagaa gcggaatggc ccggtcgagt
    18061 agcccgtata ccatctggcg aaaatggctt tgggtatgat cctttattta tcccagaagg
    18121 taagaaacaa acagcagcag aattatcgag cgaagaaaaa aacaagatca gtcatcgggc
    18181 tcaagcaatg aaaaaattga gtgccgagtg gaaacaatgg ctggaaggag agagatagat
    18241 gcggtattta gtagtaagtg ataatcatgg agacagagat atcctcgttg acttagtaga
    18301 tcgttatcgt tctgaagtgg atatcttttt ccattgtggt gattcggaat tagaagcttc
    18361 tgacccatta tgggatacat tccaagtggt tcaaggtaat tgtgattatg gaccaggatt
    18421 cgaacagaaa aaagtgatcc aaactggtca ggatacagta ttcatgactc acggtcattt
    18481 atcgaatgtc cgatttggtt tgacacagtt agcaatcgaa gctcgagctg caactgccga
    18541 tatggtgttt tttggtcata cccatcaaat aggctgtgag atggatcaga aaatcctgtt
    18601 tttgaatcct ggtagcattt ctcagcctag aggcccgatc caaatcccat catatgctct
    18661 tatcgaaagt ttagatgacc aaatttctgt tcaatactat aaccgtgccc atcaggcagt
    18721 tgaagagttg gccttttctt tttcgagaga gcgctgaata aaaaaactct aatttttaat
    18781 aaaaataagc tttattttat gaattttgca tccggtttcg gtaaaatagt agtgttgaat
    18841 gaaatagaaa tggaggccat cagatgattg gacctatagt aagagaaatg ctgctggaaa
    18901 atcaggaaac gtttttgatt cctgcagaaa atgtggcaaa tgttatgtat catcatccat
    18961 tgtcccatgg tttacttgtg ttatcgaagg tcggttattc caaaattcct gtattaggaa
    19021 aagatgatca ctttgtagga ctagtaagtt tatcagatgt ggtcaataaa atgattgact
    19081 tgcaaacaat cagcatggac ccgttagaag gactaacggt tgctgatgtg acagaaacag
    19141 atgttccgac aattgatgaa aattgggaac tagaagaagt actccatttg ttagtcgatt
    19201 cgcctttttt accggttgtc acagaagatc ggattttcaa aggaatcatt acaagaaaag
    19261 agctgcttaa agccgtaaac ttcatggtgc atgaattaga acgtcgtaat atattgtctc
    19321 cgaaaatcga agaattaaaa gaacaaatcg atatcgtgag ctaaagaacg agaaactaaa
    19381 agataatcaa aaaataagta gaaaaaacag cgtaccagtt ttgactttat cttgaaaaag
    19441 aggctgggac aaaagcgttt aactccaaga aataagaagg aattcacgaa aattgctttt
    19501 caaatttttg tgaatttcag cttatttccg aaggagttgc ttctgcttcc gccgtttata
    19561 cgtgtttaga gcgtgggaca aaagtgttct ttacttttgt cccacgctct ttttagtgag
    19621 attcgttgca tcgtttgtcc aaattatttt ccgacagcaa ttggactgtt cggaatcgtg
    19681 aaaccagaag ctgtcaattc tttgacatag gagctcaaga actcttcttt taaggcatat
    19741 tgttttccgt tcaatgcgta gcagaccgta cgaatcgcaa aattgctgtt tccaagatcc
    19801 actaatccaa agatagaagg ttcagtttgg agctcttctt ggtatttttc agctaatgta
    19861 cgatttactc gatcaatgat ctcatagatt ttgtcatatc cttcttcagg aacaatccga
    19921 atatctaaaa gtacttgcat atttgcacga gagagattgc tgatcgtggt gatgtttcga
    19981 ttcgggatga aatggacagt accatccgtt gctttcagct gaagggtacg gataccaaca
    20041 gatacgacgg ttccttcaat cgttaagttt gctagcttga tgtagtcccc aacatccatc
    20101 tgttgttcca tgataataaa aaatccggta ataacatcac tcatgaaccc ttgggcacca
    20161 agaccgattg ccacacctgc aattccggca ccagccaaaa gtgatccgat cggcacaccg
    20221 atagcagata aaattgcata gataaaaaag aaaccaaggg tataatgaaa aatagtattt
    20281 aaaagactat gtaaagtctt taagcggctc tcgtttagaa tggctttttt agaatactga
    20341 ttaaaagact tatcaatcag atattttccc acacggtgaa gaatccaaaa aaggacgatc
    20401 aaaaaaatca gatataaact tttttggata aaggcagcca aaatggcgtc ccaatcaatc
    20461 ccgtcccaaa accgctgaaa cgcatttact ttgctgacag cttgatctgt caatgtctcg
    20521 ccagaagagt ctgtcgtacc ggcagttgct aataatgaaa acataaaatc ctcttttcta
    20581 aaatttgatt ctttctgtat tgtaccaaga agttttcaaa aaaggaaata gcaaaaataa
    20641 ctataatgaa ggaaaaaagc tgaaaataaa atgacaaata atatggataa tgataaaatg
    20701 tggagaaaat aacgaaggtg gtaagtatat gcaaatcgat tggaacaatc taggtttttc
    20761 ttatatcaaa actccttggc gttttattgc caaatggaaa gacggacaat gggaagaagg
    20821 agaattgaca gaagataatt acctgacgat tcatgaaggc tctccagcgc ttcattatgg
    20881 tcagcaatgt tttgaaggtc taaaagctta tcgtcgaaaa gacggcaagg tcaatctctt
    20941 tcgacctgaa caaaatagtc gacggatgaa tcaaagtgct agacgattgc tgatgccaat
    21001 ggtccctgaa aagatgttcg ttgatgctgt caagcaagtg gtaaaagcaa atgaagattt
    21061 tgtgccgccc tatggaacag gcgctacatt atacttgcgc cctttactga ttggggtcgg
    21121 tgaaaacatt ggtgtccatc cagctccaga atatttattt gtcgtcttct gtacgccagt
    21181 aggagcgtat ttcaaaggtg gactaaaacc aacgaatttt attgtatctg attacgatcg
    21241 tgctgctcct aaggggactg gtgcagcaaa agtcggcgga aattacgcag cgagtttact
    21301 tccaggaaca gaagcaaaag aactggatta ctcagattgt gtttatcttg atccagccac
    21361 acatacgaaa atcgaagaag taggtgcagc aaatttcttt ggaattacaa aagacggaaa
    21421 attcatcact ccgcaatcgc cctctatctt accaagtatc acgaaatatt ccttgttgca
    21481 gttagctgaa gaacgactag gactgcaagc aattgaagga gatattttca ttgatgatct
    21541 agatcaattt gcagaagctg gtgcatgtgg tacagcggct gttatttctc caatcggtgg
    21601 gatttatcac aataacaatc ttcatgtatt ttatagtgaa acggaagtag gaccagtaac
    21661 aaaacggttg tatgatgaat tgacaggtat tcaatttgga gacatagaag caccagaagg
    21721 ctggattgaa gtcgtggaat aatttcaaag taatgagttg aaaaaaacgt aaaaagaaaa
    21781 aatcaggaag agactttaaa aagttttttc ctgattttta tatgttttac tttaaagaat
    21841 gcaacaaaag tttatgaaag atggacacct ttgtcaacgt agataatatc accgatgatt
    21901 ccgctggaaa gtgggctgat taagaaagca caagcattac cgacttcttc gatcgtcaca
    21961 ccttgctgat caggcgtacg agattcagat aactggatca gtttttgata gtctttaaca
    22021 cctgtgacag ctaatgtttt gattgcccca gctgagattc cattgacccg aattccttta
    22081 ggcgagaact cagctgccaa ataacggata gcagcttcaa gagaagcttt cgcaataccc
    22141 atcatgttat aattcgggat tgcgcgttct gaacctagat aagtcatgct aacgatcccg
    22201 ctgtttggtg caagaaacgg ttgtgcgtat tttgatacag caattaaaga gtagctgctg
    22261 atatcttgag ctaattgata gccgtctctg gaaatatcag acacatttcc ggacaattct
    22321 tctttgttgg cgtaagcaac tgcatggaca aggccgtgga tctccccagc atattcaccg
    22381 atggcattca ttgctttttc gatgctttcg tcgctagaaa catcacattc tacagtgaat
    22441 gcttcttcgc cgaccaattt gactaatgat tttttcattc gttcattttg gtaagtatag
    22501 ataatttctg ccccttgttt caacattgct tcagcacaac cccaagcaat gctttttttg
    22561 ttagcgacgc ccataatgac gatttttttt ccttctagaa atgacataat gactctcctt
    22621 tgtttctgat aaaaatttga tagtcgtaaa aaaatagaga ataatagcta aaataacttc
    22681 ttgatttctt aattattagt caaaatgttt tgattgtcaa actatttttt cttaaattaa
    22741 aaaaagattg ctaaatattt aggaatatga tactttgata gtcaaagtaa tgaaaatggc
    22801 aattgcctga aagaaggagt ttaattatgt cttacgtatt atctgctact gaagtaatgg
    22861 aattgattcc taatcgttat cctatttgtt atattgatta tgtagatact ttagaaccag
    22921 gaaaaaagat cgttgcgaca aaaaatgtaa caatcaatga agattttttc caaggtcact
    22981 ttcctgacaa tccagtgatg ccgggtgcgt tgattctaga aacactggca caagcaggtt
    23041 cgatacttat cctaaaatca gaagagtttc taggaaaaag agcttatatc ggtgggatca
    23101 ataaggccaa attccgtcaa aaagttgttc caggagatgt gctgaaatgt cattttgaaa
    23161 tcaccaaaat gaagggatca gtcggtacag cgttatctgc agcatatgta gatgataaaa
    23221 aagtctgtga gtgcgagttc acgttcatcg tcaaccaaaa tgcttgacta taaaacctga
    23281 tatagcaaaa caaaaggaag cttacttgct ttttatgtaa aaagaagacg cctgaatcct
    23341 agcatacgtt gtcatctatg caggattcag acgttatttt tttatttttg ttttgcttct
    23401 tgcagtatat tgattttatc gatttttact tcttcttttg gtttgtctga actatctgtt
    23461 tcgatatttg caatagcatc cacgacatcc attccttcaa tcacttggcc gaagacagta
    23521 taatggccat ctagtgaagg ataaccgccg tttttataag cgtcgatgat tgcttgagga
    23581 tagtcgtctt ttaaaagacc atcatgcata tcatctgtat tttgaacgat aaagaactgg
    23641 ctgccgttac tgttaggatc ttgtgagcga gccatagata acgcacctct tatattataa
    23701 aggtggttgt tgatctctgt ttcaaatcct tctccccaaa tgctttcccc tccagtgcca
    23761 tcgcctttag gatcgccacc ttggatcatg aaatctttga tgacgcgatg gaaagtcagt
    23821 ccgttgtagt aaccgtcttt tgcatgtgtc ataaagtttt caaccgcttt tggtgcgtgt
    23881 tcgggaaata atttgatttt gattgtgcct ttattcgtca ccatttcgac cagatcttca
    23941 ttttcggcga catctttcgt caattgtggt aattccaatg cgttcaaatc gacgctactg
    24001 gcagtcgtgc tttccgcagt agatgtactt tttgaacttt ctgtagacga actagaagaa
    24061 gtcgtgttgt ttgcagaaca accagcagca aacagcaatg ctgctgctaa actaatactt
    24121 aaaataactt tatttttctt cataactgtt cctttccaat ttactatttt gttatcttta
    24181 atgataacaa aatagaggga aaatacctac ttaaattatt ggaatttatc atttatttta
    24241 aaagaaaaag aaaaggtgta gaccatttac tttgtgtttg ctataatgta agtggataca
    24301 aaaacgaaaa gatgcaggag gaaatggtaa tatgggaaaa ttaggaatct cgatttatcc
    24361 agaacggtca acatttgaaa aggacaaagc gtacttggat ttagctcata agtatgggta
    24421 taaacgggtt tttaccagtt tgctgcagat caatgatgat aaagaaaaag ttctttcaga
    24481 atttaaagaa gtagttgatt acgcgaatag cctaggcatg gaagtaatgg ttgatatcaa
    24541 tccagcgtta tttgaacagt tggagatttc ttacgatgac ttatcttttt tccataagat
    24601 gggtgcctac ggggtccgct tagatatcgg attcacagga gcagaagaag caaaaatgac
    24661 gcgcaatcct tttggtatca aaatcgagat caatatgagt tcgggcacga attacgttga
    24721 caacatcatg agttattcgc caaacacgga taatttatta ggttctcata atttttatcc
    24781 tcatcgatac tctggtttag gatacgaaca ctttgttttt tgttcagaaa aattcagaaa
    24841 atataatttg aatacgatgg cattcgtcaa ttcgcaatct gctgagtttg gcccttggcc
    24901 tacgcaagat ggcttgtgca cgctggaaga ccaccgagat cttgaaattg cgacacaagt
    24961 gaagcattta atccttacag gattgattga tgatatttct attggaaatg cgtacgcaag
    25021 tgaagaagaa ttgaaagaaa tggcagaagc atttaacgcg gattatccaa cattgaaagt
    25081 agatacggaa gaaggtatta cagaaaacga acgaatctgc ttgtttgata accttcatag
    25141 ttatcgaggg gatcgctcag aatatatcct tcgttcaacg atgactcgtg tatactacaa
    25201 agataaagat ttcccgcctc ataatacgcg agatatgcat catggggatg tactgattga
    25261 caatgaaggg tacggtcaat acaaaggaga aacacaaatc gccttgaagg atatgaaaaa
    25321 cgacggacgt gtcaatgttg taggaagaat ctccgatgac gaattattcc tccttgactt
    25381 tttgaaacca tggtcaagct ttaaattaat tgaaaataaa aaatagataa ttagttattg
    25441 cagaaataat ccgctagaaa tagccagtta tttctgcttt tttattttgt ttttttatag
    25501 gttatgattt gtcaaattaa gct
//
LOCUS       DAMYXM010000033        24574 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.034, whole genome shotgun sequence.
ACCESSION   DAMYXM010000033 DAMYXM010000000
VERSION     DAMYXM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.034"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(153..551)
                     /locus_tag="PNS21_001571"
     CDS             complement(153..551)
                     /locus_tag="PNS21_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDI5159283.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="PNS21_001572"
     CDS             complement(548..1321)
                     /locus_tag="PNS21_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDI5159284.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="PNS21_001573"
     CDS             complement(1318..2001)
                     /locus_tag="PNS21_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDI5159285.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="PNS21_001574"
     CDS             complement(2168..3055)
                     /locus_tag="PNS21_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDI5159286.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="PNS21_001575"
     CDS             complement(3052..3921)
                     /locus_tag="PNS21_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDI5159287.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5344)
                     /locus_tag="PNS21_001576"
     CDS             complement(4058..5344)
                     /locus_tag="PNS21_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI5159288.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5617..6195
                     /locus_tag="PNS21_001577"
     CDS             5617..6195
                     /locus_tag="PNS21_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDI5159289.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6195..7124
                     /locus_tag="PNS21_001578"
     CDS             6195..7124
                     /locus_tag="PNS21_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI5159290.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7121..8857
                     /locus_tag="PNS21_001579"
     CDS             7121..8857
                     /locus_tag="PNS21_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDI5159291.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="PNS21_001580"
     CDS             complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="PNS21_001580"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDI5159292.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9753..10262)
                     /locus_tag="PNS21_001581"
     CDS             complement(9753..10262)
                     /locus_tag="PNS21_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDI5159293.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="PNS21_001582"
     CDS             complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="PNS21_001582"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDI5159294.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11563..12654)
                     /locus_tag="PNS21_001583"
     CDS             complement(11563..12654)
                     /locus_tag="PNS21_001583"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDI5159295.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="PNS21_001584"
     CDS             complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="PNS21_001584"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDI5159296.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="PNS21_001585"
     CDS             complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="PNS21_001585"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDI5159297.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="PNS21_001586"
     CDS             complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="PNS21_001586"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDI5159298.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16617..17591)
                     /locus_tag="PNS21_001587"
     CDS             complement(16617..17591)
                     /locus_tag="PNS21_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HDI5159299.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17572..19455)
                     /locus_tag="PNS21_001588"
     CDS             complement(17572..19455)
                     /locus_tag="PNS21_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HDI5159300.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19473..21698)
                     /locus_tag="PNS21_001589"
     CDS             complement(19473..21698)
                     /locus_tag="PNS21_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDI5159301.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21813..22847
                     /locus_tag="PNS21_001590"
     CDS             21813..22847
                     /locus_tag="PNS21_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI5159302.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22914..23927)
                     /locus_tag="PNS21_001591"
     CDS             complement(22914..23927)
                     /locus_tag="PNS21_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI5159303.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24005..24193)
                     /locus_tag="PNS21_001592"
     CDS             complement(24005..24193)
                     /locus_tag="PNS21_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDI5159304.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7061 a         5342 c         4397 g         7774 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atacaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5341 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5401 caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat
     5461 aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga
     5521 aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt
     5581 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
     5641 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
     5701 tttcaattaa tagggacttt ttccagtccg ctgaaagcgc ttgatttttc tttggtacat
     5761 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
     5821 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
     5881 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
     5941 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
     6001 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
     6061 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
     6121 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
     6181 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
     6241 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     6301 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     6361 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     6421 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     6481 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     6541 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     6601 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     6661 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     6721 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     6781 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     6841 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     6901 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     6961 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     7021 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     7081 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     7141 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     7201 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     7261 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     7321 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     7381 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     7441 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     7501 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     7561 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     7621 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     7681 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     7741 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     7801 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     7861 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     7921 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     7981 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     8041 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     8101 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     8161 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     8221 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     8281 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     8341 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     8401 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     8461 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     8521 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     8581 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     8641 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     8701 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     8761 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     8821 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     8881 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
     8941 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
     9001 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
     9061 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
     9121 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
     9181 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
     9241 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
     9301 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
     9361 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
     9421 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgc gaaaggattt ttgtaatctt
     9481 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
     9541 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
     9601 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
     9661 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
     9721 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
     9781 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
     9841 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
     9901 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
     9961 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
    10021 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
    10081 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
    10141 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
    10201 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
    10261 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
    10321 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
    10381 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
    10441 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
    10501 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
    10561 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
    10621 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
    10681 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
    10741 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
    10801 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
    10861 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
    10921 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
    10981 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
    11041 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
    11101 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
    11161 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
    11221 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
    11281 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
    11341 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
    11401 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
    11461 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
    11521 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
    11581 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
    11641 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
    11701 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
    11761 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
    11821 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
    11881 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
    11941 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
    12001 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
    12061 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
    12121 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
    12181 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
    12241 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
    12301 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
    12361 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
    12421 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
    12481 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
    12541 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
    12601 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
    12661 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
    12721 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
    12781 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
    12841 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
    12901 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
    12961 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
    13021 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
    13081 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
    13141 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
    13201 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
    13261 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
    13321 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
    13381 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
    13441 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
    13501 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
    13561 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
    13621 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
    13681 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
    13741 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
    13801 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
    13861 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
    13921 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
    13981 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
    14041 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
    14101 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
    14161 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
    14221 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
    14281 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
    14341 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
    14401 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
    14461 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
    14521 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
    14581 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
    14641 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
    14701 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
    14761 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
    14821 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
    14881 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
    14941 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
    15001 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
    15061 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
    15121 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
    15181 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
    15241 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
    15301 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
    15361 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
    15421 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
    15481 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
    15541 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    15601 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    15661 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    15721 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    15781 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    15841 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    15901 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    15961 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    16021 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    16081 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    16141 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    16201 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    16261 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
    16321 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg
    16381 taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt tatgaaactt
    16441 tttttaattt tctgatagtt aactttgttt ttcagaaaaa taacaattgg ttttattgaa
    16501 gaaaatagtc atgaacaaaa gtatctgaga acttttttac tctcttgttc atgacatata
    16561 gaataaagca gctttgtctt gtaaactccg tttttcaaat aataatctaa agattgctac
    16621 tgaataataa agccgatttc atggtcctct tcgctgctga ttcggtaagg tgactcaaca
    16681 tccgctcccc agctattgat acccccgact cctcttactt ttgcaaaaat cgtaagaacc
    16741 gttcgtcttt ctggcggtag ttcttctata tgtgttgcct gttccagttc ttctgctttg
    16801 aatggtaaag cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct
    16861 tcttgatctt tatccgtatt atccaacgtt tgatttcttg ttagttctac ccatttcgtt
    16921 tccatatgca tgccgcattc ttggggaaca aggtatgggg aaacggggag tcctgccact
    16981 ttataaatac cttcgacacc accagccatc cgatccggat atgtttcccc tgataggcct
    17041 tcatactcaa aaaattttgc tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat
    17101 tccggtaatc ctttttgtcc ataataatgc atattgacaa gcattttacc ctctccagcg
    17161 atttggtagg aaattgtcac ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg
    17221 atttcaaccg tttcagcgta ttcattactt gaatattgat tatttactgg agcaatcggc
    17281 aaatcgatct ttttattgtc caccagtact tctatttctt tgacttttgg atacaatccg
    17341 gctcctaacc acataccgga tttaaatcca aatccgctac ctctgtcatt atcggtcgtt
    17401 gctcgccaaa aaactggaag tgtttcccgg tatagccatt cttttccgtt tttattgagc
    17461 gattccagtc cgcctcttgt ataagaaaaa atataatgcg tattccccga tttgacacct
    17521 aatgtacagt cgccgaagac gattgcaact ttttccgtat ccatatgact tttatttact
    17581 gtatttttca taataatatc ttacctcctg catggcgggt ttttcttgtc catctgcaaa
    17641 aacgatccca ttccctgaga attcatagtc acttggttta tcgtcaaagt caccgccata
    17701 gcgcatcact tctctgcctg tgattggatc agtcacttgg atcgcctgat ctttaaaatc
    17761 ccatatatag cctccttggt acatttcata acgatcaagc aacgtcatgt aagaatccat
    17821 tccacctaac gaattcccca tgtcatgcat atattcacat aagatgaacg gtttttccgc
    17881 tttattttca aggtagtcaa tgatctcttc tggtggcgca tacatgcagc tttttacatc
    17941 tgaaatcgaa tcttcgaatg ctggatttgg aaaattccct tcataatgga caagtcgatc
    18001 ggggtctact tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg cataagattc
    18061 atttccaaga gaccaaaaaa gaatacttgg atggtttttt aatgtttcga aattcgtttt
    18121 tgctcgatca agaacagccg cctgccactg aggtactgca cctggtacgt tgtaagaagg
    18181 ctcaaatgcg cccatttttt gccatgagcc atgggattcg aggttcgttt ccgccatcac
    18241 atggattcct gcttggtcgc ataaataata ccaagtgatc tggttggggt aatgagatgt
    18301 ccgtacgccg ttgatctttg cattttttat gatagccaaa tctttttcca tatcttctat
    18361 agtgatggcg cgtccttttt tcggatgcca ctcatgtcga tttaccccgt tgataatcat
    18421 ccgttttcca ttgaagtaga tgattttatc tttgatttcg attttacgaa aaccgaagga
    18481 atagtcaata tactccgtta ctcttttttc ttcgtctatc gtctccaatt caagcttata
    18541 taaattgggc tgaaaatgcc cccacagtgc cacattttca aatccatcga tgcgaatcgt
    18601 catttgctct tttaacggat attgctgttg atagatttcc ttcccatcag gatctagtat
    18661 cgctacattc agctgactgt ttttggctac ttgctcaaaa gacaaatcta cttcaagttt
    18721 gccattttga catttgtcat ctaagcgagg tttgatccaa agatcttcta aatggttttg
    18781 tcctcgagca actaagaaga catctctgaa gatcccaaaa aatcggaaaa agtcctgatc
    18841 ttccaagaat gcagctgtac ttcttttgta aacctccaca gctagaatat tttcttcttc
    18901 caaataaggt gttaaatcaa attcagacgg agtgaacgaa tcttcggcat acccgataaa
    18961 atgaccattc aaccaaataa acattgcttg ttctacacca gcaaaatgaa tcgagatttg
    19021 ttttcccttt agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc
    19081 tttagcagct gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga aagggggacg
    19141 gcgaaataca tgtccctccc acggatacat cgtattgatg taatggatct tgtcataatt
    19201 tgctaattca atatgactag gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt
    19261 tttataaaat gtttttggtc gtaccgaagg atttggcgaa taacaaaaac gccatgatcc
    19321 atttaaagac tgtttaaggg aggatgtggt ttctacttct tcccttgttg cataacaaac
    19381 atgatcgcta tgtgcatttg attggttcac tctaaaaact tgtggatcat ctaaccattt
    19441 tatatttgct ttcatacttt tttccttctc tctcataact ttttgaaatg aagcagttct
    19501 gtttggaaat caccttgaaa atcggggaag taaagtccaa ctgtcatcaa ttcggccccc
    19561 gaaaatatcc gcttcgaggt ctgttcctga taaagtgcct catcatctaa ccccatcaga
    19621 tatacttcat ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa cacaatagct
    19681 tcatcttgtt taggagacac aaacatccat gcggcaatat tttcttcaaa aggactcgcc
    19741 aaacgataat aatggccata ctggatcacc ggacgaatcg tttgatagga tataatttgg
    19801 tcttttactt gctttttttc ttcaggagac agctttgtca aatccagttc ataaccaaaa
    19861 acagcgctca tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga
    19921 tttggtacag ctgatacatg agctgtgatg gacgaaatcg gataagcaag gcttgttcca
    19981 tattggattt tcatacgctc cactgcatcc gtgttatcac ttgtccatga ctgcggcata
    20041 taataaataa acccggcatc aaaccgtcca cctccaccag aacagccttc ccataagatt
    20101 tccggataat cagttgtcaa atgttcaagt agttcataaa gcccgagcat ataacgatgc
    20161 gctacttctc cttgtctctg tgggtcaagg gtaatcgaat agacatctga aagcgaacgg
    20221 ttcatatccc atttgatgta gtcaagagga atcgtatcta aaatctttct catttgtttc
    20281 tcaatcgtct gccgaacatc taaccttgta aaatccagta catgttgtga tctggaagca
    20341 gaaggcattc tacctggctc ctgcataaga aaatcaggat gtgttcgata cagttcagaa
    20401 tcaaccgaga tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt gctatgagca
    20461 taatctgcta tttcacgtaa tccatttgtc aacttgcctt catactcgaa ccagtctcct
    20521 aatgaagaat tgtccgcatc tctttttcca aaccagccat catctaatac gaacatttcg
    20581 atgcctagct cctttgcttc atcaatgatc gccttgattt tttcactatt gaagtcaaag
    20641 taagtggctt cccagttatt gaccagaatc ggccgttcag caaattgatg acgacctctt
    20701 gcgattcttt cacgtagcaa ctcatggtgg atctgactca ttttgttcaa tccctgactt
    20761 gagtaagaaa ggatcatttc aggtgtttga aaagactggc ctgcattcaa ttcccacgaa
    20821 aaacgatggc tgttgatccc accgacgact ctcaattgat tgatctgatc tttttcaagt
    20881 tcgaaagaat ggttgccgga ataaacaaat tgaagtccga tcgcttctcc agtattttct
    20941 gtcgtatgat gatgaactaa agcaataaag ttattcatat ggtgactgct cgttcctcga
    21001 cggctttcaa atacttttgt tccagataaa acagattgcc gtgaaatctg tctttcattg
    21061 acatgtgctc ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc aaagtctagc
    21121 tggaaactag ccgctttttc tagtttgatt gtttctccgg tttcatttct tatctttaca
    21181 gatcgtgtga tcactgctct atcagtaaat atggtataga acaaatcgaa ataaatcgct
    21241 gtttctctat ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc catcacataa
    21301 gtttgaggca agccttcgat ctctggcttc ccatctgtaa tataatggct gtcatatctc
    21361 agatccagta agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc
    21421 atgctgccat gagaagagta ttcttgcggt agtgtatctt tcgacagaga ccggtcagga
    21481 tttaagggaa catttcctga aaatccacga tctcttcggg gataattttt attgtctttg
    21541 tactgcttga ctggtttgcc aaaataaata tgcgccaata cattgctttc ttctatcttt
    21601 atcagataag aaagaaatgt atttcgtaaa tggaagactc ggctcttttc atcaaataag
    21661 atgaaatttt tatctgtttt tctatgcgta atatccatca tttcactccc aacaaaacgg
    21721 aaaccgttta ctttttttta taattcctgt atactgttta ctaaacaatt ttgtcaataa
    21781 aaatttttta tttttttgag gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa
    21841 aggcaaatgt ctctcctgct acagtttccc gtgtgttgaa taacgatcag acgatccatg
    21901 tggctcagac tactcggcaa cggatagtag aggcagctgc tgcgttggat tatgacatca
    21961 gtactcgtaa atatatcaaa aagagaatgc cttcgattgg tgtgatctct acgatcggca
    22021 aagcgagcga agaacaggat atctattaca aacatttgcg agtagggatg gaagaagaag
    22081 ctcgacgtct gcatttaggg atgaatcgaa tctataatct atctgataat cctaaacaat
    22141 gggaagatct agatcagtta ggtgcggtga tcgttgttgg gactgttacg aaagaatcga
    22201 ttaccgcctt gttaaaacaa aacatgcaaa tagtcgtcgt ggataaccct gacatccagc
    22261 aagaggtcga tatggtgtat gcagatcttg aacggatgac taaaaccgta ctggaattgt
    22321 tcttagaaaa tggtcataaa agaatagcct atatcggggg atataaagtc gatatggatg
    22381 aattcggtgg gaagcagatt tctataaatg aaaagaggat gcgtgcctat cagcatttta
    22441 tgatcgatca tcacttagaa aaatatatgt cttatcatct aggtggctgg acagaaaaac
    22501 aaggtgaggt acttgcgact aatctattag aaaatgaatc ccctcttcct actgccattc
    22561 ttgtcgggag cgatccaatg gcaattggtg tctatcgggc attgaaacaa aaaaatatcc
    22621 aaattggaga agagattgtt gtagcaagct ttgataatat cgagacaatc gcaaacctct
    22681 cccctggctt gaccagtgtt caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa
    22741 tggcattgga gcgaattgac ggtatacggg acgaaccaat cattttgact tatcctgcac
    22801 gattgattgt tagggaaagt tttatcccaa gaacaaaaag gtattgaaag agaggttgtg
    22861 aaaagccgct tcgtttcttc atactcaaaa cgaaacggct ttttcacaac ctcttactta
    22921 tacttattca caaaatccaa tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa
    22981 tcaaattggt attcatgggt aatctctttt tttgtatctg tataaaacaa ggattgcacg
    23041 ggtaccttta attctgtcaa ccgattttct aatgcttgac cttgctcctg gaatgagtat
    23101 gcatttccgt cagttatata cgttggcgga aattccttag aaacatggtc agccacacta
    23161 gcttctttca attgctcact cgttttccaa tcttttgttc caatcaacga ccacgcaacg
    23221 gttttgacga aaaacttcat aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa
    23281 tcaacaggtc cgcaataaga aatggttcct ttcagactat cttttggaag tagctgtttg
    23341 attttcattt gtttagcata cgcatcattc gtttgtatag ctgcatattg caatgcgatt
    23401 tgtgaacctg cactatctcc accaaaaaat aattgttcca tgtctagcaa tttgtcgtct
    23461 ttttcttcag ctaataaatc tttgatcagt tcattcactt gaagaacttg attagggtat
    23521 tctgaatcag gcgctaattc ataattcatc gcaaccactg cgatgttggc gtctgaaact
    23581 aattttgtcg caaattcttt gacacccgat ttatctccgc cgacgaatcc tccaccatgt
    23641 acccaaaata gaacaggaac aggccccttg ctgttttttg gatagtagat gtcataagta
    23701 ttctctttat acttggactc atacgtcaaa tctgtttttt tcgccacgtt tttttctgct
    23761 tgatcgaacg tttttttatc agtgatttga acagtatttg aaaacatatg gttgatcaag
    23821 aatgctccag gtctggggga aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga
    23881 atgattccga agaaccatcc gataaattta agaattttct ttttcataag gctccctcag
    23941 tagttagtat taagcattct gattatacca tagtgttatt tcctatcaag ctctcctaga
    24001 tgatttaatg attttcccca aacttcttct gcaatgcttt tgacaaaaga aattttttcc
    24061 attgttcttc aaacaaaaca ttttcttctt ctgttgaagc aaatccacat tgaggactta
    24121 agcacaaacg atccaaaggc agatatcgtg tcgcttcgta gatccggtca atcacttgct
    24181 ggtgctcttc catttcgcca tttttcgtta cagtcaaaat ctaagacaac taatttatct
    24241 tcagagactt tctctaaaag aacaaaactc ccagcacgtt cactatcgta ttctaaataa
    24301 taggcacgga cattttcttg agtaaaataa tgggtctgct acgtcgtcat cccccctgat
    24361 gccgccaaaa tcgaataata atttccgcgt caaacatgta tatttagtgt tgaatcattt
    24421 gagaatatga aaataaaaat ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa
    24481 atctgagaca gaatgacgtt cattctgacc cagatttgat tttgcacaac tcaacagtcc
    24541 ttattggcaa atcgccaaaa taatgtagac taac
//
LOCUS       DAMYXM010000034        24379 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.035, whole genome shotgun sequence.
ACCESSION   DAMYXM010000034 DAMYXM010000000
VERSION     DAMYXM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.035"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(36..884)
                     /locus_tag="PNS21_001593"
     CDS             complement(36..884)
                     /locus_tag="PNS21_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDI5159305.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSLKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1108..3048)
                     /gene="thrS"
                     /locus_tag="PNS21_001594"
     CDS             complement(1108..3048)
                     /gene="thrS"
                     /locus_tag="PNS21_001594"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDI5159306.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3385..3564)
                     /locus_tag="PNS21_001595"
     CDS             complement(3385..3564)
                     /locus_tag="PNS21_001595"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDI5159307.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3611..5434)
                     /gene="cadA"
                     /locus_tag="PNS21_001596"
     CDS             complement(3611..5434)
                     /gene="cadA"
                     /locus_tag="PNS21_001596"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDI5159308.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5712..7991
                     /locus_tag="PNS21_001597"
     CDS             5712..7991
                     /locus_tag="PNS21_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI5159309.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8084..8731)
                     /locus_tag="PNS21_001598"
     CDS             complement(8084..8731)
                     /locus_tag="PNS21_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159310.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8910..9629)
                     /locus_tag="PNS21_001599"
     CDS             complement(8910..9629)
                     /locus_tag="PNS21_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI5159311.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9760..10911)
                     /locus_tag="PNS21_001600"
     CDS             complement(9760..10911)
                     /locus_tag="PNS21_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDI5159312.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11128..11868)
                     /locus_tag="PNS21_001601"
     CDS             complement(11128..11868)
                     /locus_tag="PNS21_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI5159313.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11872..12195)
                     /gene="rsfS"
                     /locus_tag="PNS21_001602"
     CDS             complement(11872..12195)
                     /gene="rsfS"
                     /locus_tag="PNS21_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDI5159314.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12252..12845)
                     /gene="yqeK"
                     /locus_tag="PNS21_001603"
     CDS             complement(12252..12845)
                     /gene="yqeK"
                     /locus_tag="PNS21_001603"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDI5159315.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12829..13479)
                     /locus_tag="PNS21_001604"
     CDS             complement(12829..13479)
                     /locus_tag="PNS21_001604"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDI5159316.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13489..13800)
                     /gene="yhbY"
                     /locus_tag="PNS21_001605"
     CDS             complement(13489..13800)
                     /gene="yhbY"
                     /locus_tag="PNS21_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDI5159317.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13816..14925)
                     /gene="yqeH"
                     /locus_tag="PNS21_001606"
     CDS             complement(13816..14925)
                     /gene="yqeH"
                     /locus_tag="PNS21_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDI5159318.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14922..15449)
                     /locus_tag="PNS21_001607"
     CDS             complement(14922..15449)
                     /locus_tag="PNS21_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDI5159319.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15629..16414)
                     /locus_tag="PNS21_001608"
     CDS             complement(15629..16414)
                     /locus_tag="PNS21_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDI5159320.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16407..17276)
                     /locus_tag="PNS21_001609"
     CDS             complement(16407..17276)
                     /locus_tag="PNS21_001609"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDI5159321.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17277..18656)
                     /locus_tag="PNS21_001610"
     CDS             complement(17277..18656)
                     /locus_tag="PNS21_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDI5159322.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18664..19083)
                     /gene="fabZ"
                     /locus_tag="PNS21_001611"
     CDS             complement(18664..19083)
                     /gene="fabZ"
                     /locus_tag="PNS21_001611"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDI5159323.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19108..19584)
                     /gene="accB"
                     /locus_tag="PNS21_001612"
     CDS             complement(19108..19584)
                     /gene="accB"
                     /locus_tag="PNS21_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDI5159324.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19587..20822)
                     /gene="fabF"
                     /locus_tag="PNS21_001613"
     CDS             complement(19587..20822)
                     /gene="fabF"
                     /locus_tag="PNS21_001613"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDI5159325.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20841..21578)
                     /gene="fabG"
                     /locus_tag="PNS21_001614"
     CDS             complement(20841..21578)
                     /gene="fabG"
                     /locus_tag="PNS21_001614"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDI5159326.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21590..22507)
                     /gene="fabD"
                     /locus_tag="PNS21_001615"
     CDS             complement(21590..22507)
                     /gene="fabD"
                     /locus_tag="PNS21_001615"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDI5159327.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22614..22838)
                     /locus_tag="PNS21_001616"
     CDS             complement(22614..22838)
                     /locus_tag="PNS21_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDI5159328.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22891..23853)
                     /locus_tag="PNS21_001617"
     CDS             complement(22891..23853)
                     /locus_tag="PNS21_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDI5159329.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23863..24303)
                     /locus_tag="PNS21_001618"
     CDS             complement(23863..24303)
                     /locus_tag="PNS21_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDI5159330.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6671 a         5429 c         4247 g         8032 t
ORIGIN      
        1 attctaaaaa acttatataa aaaatagtaa ggtttttatt tcgaatccat aaaatattct
       61 tggattgctt ctcgtgtttt tttcactttt tcgctaagct tttcgttttt agccatatag
      121 aaataaaaaa tgatatcgat cgtagcaaat tgggccaaaa gtgaattagt tgccgcactt
      181 cgattcgtga tttcttttgg tctagatgtt tgtacgagta catcggctat cttgctaagc
      241 gggttatttc caaattgcgt caacgcaatg atttcgatat tcatgctttt cgccagctcg
      301 gctaatgcga ctacatctgc acttctacct gagtttgaaa caagccaaag cactttcttt
      361 ttctcattgc tgacaagttg cgggagcaag acgtggatat ctttttcaaa aatgattggc
      421 ttaccgacac gagaccattt ttgcagaata tcttctacta ctaaagaaga ggcgccaacc
      481 ccatataaaa aaacagtctc tgccgattcc aaacactcta cgactttttg gatcgtttct
      541 tcttctagaa tatctactgt ttcttttatc gctacttgtg cattgttacc aagtttgttt
      601 ttgagagagt aaaaggactc atttgcttct atatcactaa aaccttcatc gattccagtc
      661 tgtagttctg ttgaaatcgt gattttgaag tccgtcagac tttgaaaacc gatttttttg
      721 gaaaaacgta caactgtcgg cgcactcaac ccggaagctt ctgctaattc attagctgtc
      781 attgtaggga ccttctcaat attaccgata atgtagttgg ccaacttttt ttctgctgaa
      841 gaataatctt ccatcatact ttcaatacga ctaattacgc tcataagttc ctccaccctt
      901 ttaatgatag tctatgtaag tataccatat cagaccctta ttcacggtcg tatactggat
      961 aaaaaacaaa aaaacaatcc gcccaacaga atatcttatc ccatcaatcc cacagattct
     1021 attgagcatc tgtgtctttg aggaatacct gttatctatt gttcggattg ctttctatct
     1081 actacttttt tacaggaaat ttacctatta gttttctctg ctgtaatttt tgacttctgc
     1141 ttccatagca tcaataaaga tattcaaatc ttctacagct gtttctttac ttccataacg
     1201 acgtacgttc actgttgcat cttccaattc cttgtctcca acaactaatt ggtaagggat
     1261 cttttgtgtt tgagaggcac gtatcttata gcccattttt tcattacgat catcaacttc
     1321 tacacgcaac cccttcattt gcaaacgttc tttgatttca taagcataat cgccatgtgc
     1381 atcaacagaa acagggatga ttgttgcttg tataggtgcc aaccatgttg gaaaagcgcc
     1441 tttgtatact tctgttaaat aagcgacaaa acgttccatt gtagaaacaa ttccgcggtg
     1501 gataactact ggacgatgcg tattttcccc gtcttctcca acatatgtca aatcaaagcg
     1561 ctctggcaat aagaagtcta attgtatagt tgataatgtt tcttctgtac caagagctgt
     1621 tttgacttga acatctagtt ttggtccgta aaaggctgcc tcgccttctg cttcaaagta
     1681 atctagttct aattcatcca tagcggcttt cagcattgct tgggcttttt cccacattgc
     1741 gtcatcatca aaatatttat ctgtattgtt tggatcgcga tagctcaagc ggaaacgata
     1801 atctgtaata ttgaagtcag catatactgc caccatcaat tcaagcgtac gtttaaattc
     1861 atctttgatt tgatcaggac gaacaaatgt atgaccatca ttcaacgtca tttctcgtac
     1921 acgttgtaaa cctgacaaag cacctgattt ttcatagcga tgcatcattc ctaattcagc
     1981 gatacgaatt ggtaattcac gataagaatg gatcgtgttt ttgtagacca tcatgtgatg
     2041 agggcagttc attgggcgaa gaacaagcat ttcgccatct cccatatcca ttggcgggaa
     2101 catgtcttct tgataatgat cccagtgtcc agatgttttg tacaactcaa catctcccat
     2161 tattggtgta tatacatgtt ggtaacctag actaacttct ttgtctacga tataacgttc
     2221 gatcgtgcgt cggattgttg ccccttttgg aagccagaat ggcaaaccag aacctacttc
     2281 tggggaaacc atgaacaagt caagttcttt tcctagttta cgatggtctc tttcttttgc
     2341 ttcttcacgg agacggatat attctttcaa agctttcttg tcaaaaaagg ctgtcccgta
     2401 tacacgttgc atcatttgat tgtttgagtt tccacgccaa taagcacctg ccactgataa
     2461 taatttaaat acttgtatac ggccagtgga tggaacatgt ggtccacgac acaaatcgat
     2521 gaattcgcct tgttgataag cagtaatcac ttcttcttct ggcaattcac taatcagttc
     2581 tactttgtac ggatcacttg caaaaatttc taatgcttcc tgttttgaaa gaacacggcg
     2641 ttcgatcggc agattttctt tgacgatttt catcatttct gcttcgattg ccggcaaatc
     2701 ttctgcagtt acttggtttt gtccattatc tgtatcgtag taaaaccctg aatcgatagc
     2761 tggtccaacg ccaaagtgga tatttggata gaggcgacgc atggcttggg ccatcaaatg
     2821 agctgctgaa tgacggacta agccaagtgc atcttcatgg tctggtgtga tgatctcaat
     2881 acttgcatct tcctcgattg gacgagtcaa atcaacgagt tttccgttca ctttaccagc
     2941 taaagctttt tttgctaagc tttttgaaat gctttcagca attgccaatg ttgttgttcc
     3001 agactcaaat tctttgactg caccatctgg aaaagtaatt tttatcataa tagtttcctc
     3061 cttgaaatgt tttgattgat cagatcatca agctgaaaaa caaaaaaatc ctagcatctc
     3121 ttgaaaagag atactaggac gaaaagttcg tggttccacc taatgttcaa acgcaaatgc
     3181 gttcctcaag cgttgtaacg gtccgaccgc ccttgcttca acaagggttt cgaaagtggt
     3241 catcctttgg ttttctcctg gaaaagtttc agcctagctt ttcctctctt atcgaagact
     3301 ctcaaattca gttggctttc tcatcaatca attatgtgtt tatttaacca ctaataaata
     3361 gaaaaatcaa gtgggaaact aaacttattt tttccattcc ccatttacac cgtatgttcc
     3421 ttttttcatc tcattgatga tgacatggat gtgttctttt ggtgcctgcg tgtttttatg
     3481 aacagcttcc gtgatatctt tcatcatatt ttctaattgt tccggactgc gaccttctac
     3541 taattcgaca tgtacgaatg gcataatgct ctctcctctt tctgatcgtt cttttttcaa
     3601 ctcgattcta tcatactatg tttgtcttgg gtactgaatc aggaaaaatg gctggatctt
     3661 tttttgctct caaggcactc aaaatagaga ccgtatccac gacttcttgc aaaacagctc
     3721 cgaataatgc ggggattagt cccgtacttg caattaacat caaaaaaata caaataaaga
     3781 tgccaatcag tacagactgt ttcgctattc tcatcgtatc tcttgaaatc actactgctt
     3841 ttgctacacg caagagatca tctttcaaga tgaccgcatc tgcactttca ctagcagctg
     3901 tagaaccatg agcccccatt gcaataccga tatctgcagc agcaagtgct ggtgcgtcat
     3961 tcacgccgtc tcctaccatc gcaacaggtc tttgatcttt agggatactt tctaaaatgg
     4021 tcagtttatc ttgaggcaag ctacgggcat gtacagaatc taattggact ttttctgcaa
     4081 tcgtttgagc aatggcagat ccatccccgg tgatcatcat caaatttttc acatcaagtt
     4141 ttcttagctc cttgattgtc tttgctgctt ctgggcgaat ctgatccgaa aataggatcg
     4201 ttcctgcaaa agatcgatca attgttacat agattgctgt attggacgtt tctatttctt
     4261 cagctgaaac aaaagaggca cgtccaatct ttacttcatg attattgact actgcttgga
     4321 ttccttgacc ggtcacttct ttcagttgac taacaggata cagttggctg tttttcacgt
     4381 tgttcactaa cgaacgagct aagatatggc tagattcctg ttctgcacta gctgcataca
     4441 gaagtagctc ttcttttttt attccattga ctggctgaat cgcctctact tccaatatcc
     4501 ctttggtaag cgttcctgtt ttatcaaaag caatcgtttt tgtacgtgcg agcttttcaa
     4561 tcgttgttcc agtttttaca acgatcccgt ttttacttga acggctcatt cctgcaacta
     4621 aagcaatggg cgcagctaga atcagtggac aaggtgatgc cactaccagt acctcagcaa
     4681 atcgtaaagg gtcttttgag aaccaccaag aaataccacc gatcaaataa gcaatcaacg
     4741 tgaaagggac tgcataacga tcagctaaac ggacaaatcg ggcaggcttt tcttttgatt
     4801 cttcgactag tttaataatc atttgatact ggctgtctgc ggcacgtttt tcagcgatca
     4861 tttggatcga tccgtctcca tttaaagacc ctgacataat ctgatctcca ggtttcttgg
     4921 tttccgggcg ggactcacct gtcaaagaag attcatcgac cgaagattct cctttggtca
     4981 ctcgaccatc tgcgggtaca agttcgccag gcttgactag taactggtca tcaatattta
     5041 tttcatcgat tactatatcc tgcaattttc cttctttgaa tcgatgggca attcttgggg
     5101 tgttatctaa caaagactgc agttctttac ccgctcgttt attcgcgtaa tcctctagac
     5161 tatctccgcc cgtaagcatg atcaatatca tcaaactagc ccagtattct ttgacggcta
     5221 atgttgcaag gattgcggta atcgctaata catctacgcc atatcttcct gaacgtaacg
     5281 tcttgatcat ttccaaaaac atcaagaagg cgagaatccc tccagtaaat gcgacaatcg
     5341 caaatgcaag ggccggctgg tgaaaaacaa attcacttaa taaagctatc ccaccaataa
     5401 gcaaagtaag taaaaatttt ttgaaatgtg tcattgtttt ttctcctctt ttatgtgact
     5461 tagagaggtt agtaataaat ttccagctgc ctctttcatc ttaagcatag tccttgacta
     5521 gcacttttta aaacaatagg ccttaattga aaacaactat cactatcagc gtttccaaaa
     5581 ttcatcagtt ctacaaacaa aaatattcag caaggattac tttttcgatt catcagttaa
     5641 aaagtacctt ttttcgaaaa actatgctat acttttaatt cgttatttac ggcggaagga
     5701 gcaaacatgc gatgaatgag aaacaactag aacaaacgca cgtggatgaa acgatcgaac
     5761 taatccagat cgaacaaaat atgctggata caaaacaaca agaattgact caggaaatga
     5821 atcattcgac aaaagacaca gctaatcaca agattcgagc tggttccaat gaagcttttt
     5881 atgaatctgc tgttgaatat cgccaacatg agcaggaatt gcttttgaaa taccatactc
     5941 ttgaagcaca gcaaaaacgc ctgcaaacgt tgtctgtcat gaaagatagt ccttattttg
     6001 cccgtatcga ctttaaagaa gaccgtgaaa cagaaacatt atatctaggt atcgcttctt
     6061 taagagatct ggcagaagaa acaatcgtta ttgactggcg cgcaccaatt gccaatcttt
     6121 attatgaagg tgagattggt cccgcatttt atgaaactga aacagataga atagatgtcg
     6181 atctgcagtt gaaacgacaa tttaaaatcc aagaaggacg tattttatct atggtagata
     6241 cgtcggaagt aatcaatgat gactttttgc tagagatatt ggatgaatca tcaagcaacc
     6301 atatgaaaaa catcgtctct acgatacaaa aagcacaaaa tgcaatcatt cgggacacaa
     6361 caagtaaagt aatgctgatt gaaggaatcg ccggaagtgg aaagacatcc gctttgcttc
     6421 aacgtattgc ttttctgttg tatcataatc gcaagtggct cgaggctgaa aacgttcttc
     6481 tattttcacc caaccatttg ttttctgatt acatctcgac tgtattgcct tcattaggtg
     6541 aaagcggcgt gcctacacag acattcaaaa attatttggt gcagctgctg ccagagtttt
     6601 ctttactaga tgaacagcaa caagaaagtg gttttttgct aggggaaaaa gatccgatac
     6661 aagtgatgaa aagcggattg accttagtcg atcagattga tcgctatatc caaggcatca
     6721 caaactacgg tcccttgttc cgtgatatga aaataaatgg ccgtacgatc ctatcaaagg
     6781 aatcgattcg ccaatggtat aaagaaacaa atgaacaatt acctttacat catagacttt
     6841 ctctattaca aacgaagctc ctgaaaaaat taggcggtct gcaaaaagac gaaacacgtc
     6901 agcaatgggt caaagatctg gcggaagaac agctccaaga actatacgca acagatccaa
     6961 atcttgaata tacagaacaa aaggagcgtt cattacgtaa aaaactggcc aaccaaatcg
     7021 tacgtaaaag attccgtcga atccatcgag ggatcatgaa ttatcaattt gtcaatataa
     7081 cgaaacaata tctccacttt ctacaaactg tgccactttc tcttctaaca cgacataaaa
     7141 tcagcgagac caggtggcag gaacatttga caactgttcg tacacacttg cgtcaaagag
     7201 aattgcgtca agaggatgct gtactattct ttttactgat gcgccgaaca tatccagtga
     7261 cggttacaca caaagctcgc tatatcttta ttgatgaaat gcaggatttc gcaccatcac
     7321 aagtggcatt gctgcaagcc ttgttcccaa aagcaaattt gactttctgt ggagatctaa
     7381 atcaaaatgt ctttggaaat gaaacgatcg ttggatcatt ggaagacctt ttcgtggacc
     7441 gtgaagtcac tcgtttccaa ttaacgacaa gctatcgctc cacaaaagaa atcactgatt
     7501 ttgctaatca ttttattgtg aaagaacatc aagtagaaac aacagcaaga aaaggagacc
     7561 ttcctttgct catccaagga acaacagatg atatcaaatt gaattggtta gcagatacca
     7621 ttcgaacgac tcagggaaaa gcccggtatt ggcgtacagc aatcatagct aaaacacaag
     7681 aagaatgcga agctctttat gctcaactgc cagacgagtt gaaacaacaa gtccagctga
     7741 ttttaaatga gagtgacttt atgaagcgtt ccattatttt acttccagcc tttttagcaa
     7801 aaggtctgga gtttgaccga gttttcttgt gggatgtaga tgcagaacat ttttcttctc
     7861 agcaagacaa attgatcctt tacaccatgt gtacacgtgc gatgcatgaa ttgacccttt
     7921 ttacctccaa taatgaaagt cctttgttaa acaatatgga tatttcatct tatgagaaaa
     7981 aagtactgta agtaaaaaga caactgctat cattaatgtt agcagttgtc tttttattta
     8041 caataataat tgcagaaaaa gtaattcaat catttaaggt catttattct atatataaat
     8101 ccaaatctgt ttcaggatat tttaatttga caagcgaaat aatcattttc agttcttcca
     8161 tgttttctgg atattctggg tcaaatacct tgctatgacg gccaatcccg ccattgtcga
     8221 tcaatcggta aattcgattc cattcaatga attggtcaat tgattggaaa ttgctgattg
     8281 gatagccgcc acaatccaaa atctctgata cacgaaatgc taggtatgca ctatcgatca
     8341 ctttattttt tttcgtgtct atcttctcat ttctttcgat aagaattttt tcaggacgat
     8401 gtttcaagct ccactctaat agtagaaaga aagcctccat ccgatactct tcttcaaata
     8461 accgaaaggc tgaatcagaa cgacgaaaaa gcagcggaat cagataattt tgtctataaa
     8521 tcagcattgg tgcgcggtcc accatacact ctaaataacg gttcagatac cctgtaccga
     8581 catagtttcc taatcgatag ccttcaacgc cgaatcttgc gtatttttgc gctaattttg
     8641 aacaatcagt tacgttcaca gctaactctt ctactttgcg gtaaccgctt aagatacgtt
     8701 caaaaggttc taaagtgatt tttgcttcca tattcttccc cgtccatctt ccacatactt
     8761 catatcattt cacatgatac ttatgctctc atcataccac aggttgaatt agaaaagaaa
     8821 ataaatcaca caaaaaagca agacaatctg ttagtgagat tgtcttgctt ttcgaaaaag
     8881 actgaatact tttcgttaaa actggttgct tagtcttcta atgccacatt atggaacaca
     8941 tgctgtacat cttctagatc ttccagtgca tcaatcattt tttcaaatgt ttcttgatct
     9001 tctggagata gtgttacctc gttttgaggg atcatttgga tctcagcgat tgaaaaatca
     9061 gtgattcctt tttcttttaa tgcttcttgt atactatgga aatccgctac ttcaccgtaa
     9121 acgatgattt gtccttcttc ttccattaca tcgcgaacat cgatgtcttt ttccattagg
     9181 tattcaagga tctcgtcagc gtcttctcct gcaaaagcaa agattgctgt attgtcaaac
     9241 atgtaagaaa ccgctccgga aacacccatg ttcccgccat ttttcccaaa tgctgcccgt
     9301 acgtctgctg ctgttcgatt cacattgttc gtcaatgtat caacaatcac cattgagccg
     9361 cttggtccaa atccttcata gcgcaattca gagtatgttt catctcctgc accttttgct
     9421 ttttcgattg ctcggtcaat aatatgtttt ggtacattat aagttttagc gcgatcaatg
     9481 acgaaacgta gtttttggtt tgcatgcgga tcaggatcac ctgattttgc tgccgcgtag
     9541 atttcaatcc caaattttgc ataaatccgg ctattattag catctttagc agttttcttt
     9601 gctacgatat ttgcccattt acgtcccata ttttcacttc gctttctgat ttgatagttt
     9661 cataacgcaa aaacgtcctg ttccattata cttgtgaatc acgattctgt gaagcttttt
     9721 ttctttcttc cttcattctt ctcattttta gtcaattatc tatactcgga ttgggatttg
     9781 cccgaaattc tgttccggaa tattgcctcc caattgatag atttgatcac ttctaactaa
     9841 cagttccgct tcttgtgcct ctttttttcc gattcgggaa taaatagaaa tctctttttt
     9901 ttcttttaag aatcttcttc cagcatcggt aaatccaagt agtcgaagat aatttttatt
     9961 ttgttggaca ggaatctctt gctttttgat attcagcaag acataacaag ataatcgttg
    10021 gatccgtgtc catgtatatc tttttgtttt tacctttgac acaaaatcct caaaactatc
    10081 agcagttgca gctgctgttt tcatgcgcga ttctattcct tcggtcatct gatagatatc
    10141 tgtcagttcc agcaaagtag aagataacaa ccgataacgc aaatacggcc aataagattc
    10201 ccaagtctgc agcgaacaag ttgaaagatc ttcttctgtc tgaggcggca caagtccagc
    10261 tattcttttt ccttcttcta acgcatggcg aatggctgtt gcactggcta tcctctgatc
    10321 tgtgatgatt ttttcatgat agcctgcact tttacgtcgg atcggatgta attccatagg
    10381 gtggacatac ttggcatttt cctttgcata acttaatccc aagatatgat ttggcgaaga
    10441 aaaatcaagc gtaatatcgg gatacagact gcgaaaaacc tcagtcattt tttgcggata
    10501 actatacgtt tgatcagaca gttcttgaaa tgcctcttct atcctatctt gattctcttg
    10561 gacaaagcgt ccgaaactgc tgtaatcaaa cgaagtctct tcttctgttc caaaacacaa
    10621 actgtcacag tccagtgttt gcaagatttt tactgcacct ttagcaaaat agtcagctga
    10681 ctgaaccgcc cattcgacgg gaagctccac tactaaatcc acaccatttt gtaaagcttc
    10741 attcgcgcga tgccatttat cgatgatagc cggttcgcct ctttgcaaaa aattcccgct
    10801 cattacagca atgacgacct ctgctcctgt tatttttcgt gccatttcca catgatagcg
    10861 atgtccattg tgaaaaggat tgtactctac gataatcccg cagcttttca tgcgcatcct
    10921 ccttcatagt tatcgataaa aaagaaatag agacaagtaa aaaaaggcaa aaatcggaaa
    10981 aaatacccga actatataaa ctattctcgc aaacacaata cttctgcgac gaaaagctgc
    11041 actgcaagag caatgcctat atcgttcgga tatataattt atgttgaaga tttattcttt
    11101 tcttgttaac tcgagcaggc aaaaccgcta gtctttgtat gctacaaaga accagcgctt
    11161 tgatttctct gatgctgctt cgtctgtaaa atcagaaaat gcttcgactt tcgaaaagcc
    11221 gctgttttcc agcattctta aataactttc caatggatag gtacgctctt gatgtagttc
    11281 atcttcacga ataaatcgac cagacccttc ttgttcttca acgaaaaagg taagaaaatg
    11341 ttcgatgctg tgtttctcgc ttcctggata actatcccac aaaaaagcaa aattatcccc
    11401 ttgataatga tagctatatt ctggaaaaac ctcatctact tgataggtgg agtgtacgtc
    11461 aaataaaaat cggccatttt cttctaacgc ttgatagact tcatcaaaca cttgctgtac
    11521 ctcttgtcta tttttcatat agcacagcga atcagaaaaa caggtaattg cctgatattg
    11581 tccgatttct gataaatcga gcatattgcc ttccacaaat tggatcgatg cgtcttcttc
    11641 atatgctcgc tggctggcaa tcatcaacat ctcttctgaa aggtctaacc ctgtcacttt
    11701 atatccatct tttgcaaagg caaccgctaa tgctccagta ccgcatgcca gttccaaaat
    11761 attttttgtg tcttcttgta aatgacgttt cgaaaaagct aaccattgct gataaagtgt
    11821 ttcgtccatc acttcatcgt aaatgaaagc aaatgtttca taagccatat gttaatccaa
    11881 ccattcacta agatcgacta gcggagcatc tgaccatagt ttttccagat tatagaaatt
    11941 tcgttctggc gcatggaaaa catgtacgat cacatctcca agatcgatca atacccattt
    12001 tccgccttct tttccttcaa tacgttttac ttcatatttg ttttcttctt ctttatcaat
    12061 gatttcttct gtaattgcgt tgatttgacg ttcgctattt gctgaacaga tcatgaaata
    12121 atctgccaac aaggaaacat ttcgaacgtc taaagcgata atgtcttctg cccgttttga
    12181 atctgctgct tcaatcgcaa tttttaattg ttctttacta tctatgatga tttcctccta
    12241 atatgagcag cttatttttt tgctacccag taattatatg tttcgattgt ttttggataa
    12301 atctgttgtt cttgcgcaag caaatgggcc aatgtatgtt ttgtttcaaa tgctacagct
    12361 tcgtctagat cagcccatgc aatggctcgt gcatcttgta ccccaggaaa atctcgacct
    12421 ggttcaatat agtctgctac ataaatgatt ttgtcaagga gactcatctt agcagcccca
    12481 gtcgtatgca cccggattgc atgaagaatc tctgcatcgg tgattcctaa ttcccgctct
    12541 acaaacgaag cgccaacaag accatgccag attgcattat tataattcaa taactccgga
    12601 tcaaaaccgt ctcttacgat taccattttg aactcctcat ctggtcgttc ttttgcataa
    12661 tcatgtgtca atgcagcaat actagctttt tcaggagaag caccgtattt tttggcaaga
    12721 gccacggctg tttcttctac tccaagcaca tgcttgaagc gttgttcgct cattctcatc
    12781 tgtatttttt gcatcaggtt ttctctagaa taagcagtat attcattact atagtccata
    12841 ttcatacaac ccttcatttt gaatataatc aattacttta tctggaacaa ggtagcggat
    12901 agaacagcct tctttgatct tttgacgtat tttcgtggaa ctgatgtcga tctcaggtac
    12961 atctacccaa attacaggat atggagtatc cgttgtgtat cctggtcgac gtatacccac
    13021 aaagttgacc atagaagtca gctcatcgat tttataccat tttggcaaat attctaccat
    13081 atccccaccg ataataaagt aataatctgt atccggattg ttttgcgtca attccttcat
    13141 agtatcatag gtgtaacttt tcccaccacg agctaattca atcgtttcaa tctgcaaaaa
    13201 aggattgtct tccaccgcta attccaacat gttgagtcga tgttttgcat caatcgtctg
    13261 tttctcatca acatgaggtg gctgataaga aggcatcaaa aatacgcggt caagacctaa
    13321 attccggcca gcttgttcag ccatcactaa atgtgcaagg tgaacgggat tgaatgttcc
    13381 gcccaagatc cctacttgct ttttcttctc taagaattga ggcatttctt caggaaataa
    13441 ttgtgaacga acaaatgtct tagcttgcgt attcatcatg gtcactcctt atattgcttt
    13501 tacttctttt gatagccttt gatacttttc ttttgtggaa ggtttgtata atacaataac
    13561 acgtccaatg atttggacga tttcgcaatc tatttctttt tctaatactt gagctgcttc
    13621 ttctgcaatc tcatctgtat tttgaagtaa cgatactttg atcagttctc ttttttctaa
    13681 agcttcttca atttgaacga tcattgctga attgatccca ccttttccga tttgaaaaat
    13741 cggctgaaga tgatgcgctt tgctacgtaa aaaacgcttt tgttttcctc ttaaactcat
    13801 aattttcctt cttccttaaa tcaatgcttt gcgtttcaat acatctacac cctttggcgc
    13861 ccagcccgca attactactg gcgctgttac agtgatccag cctagaccgg caaaaacgat
    13921 gtctgttttt tctttcacag aaaactcaaa acgaacaagc tccggaaatg cctcgacttc
    13981 atcaggacga ggaggctgta ataatccacc tacatgtttt tgataaaatt cgtctgcttt
    14041 ttccagtttc gttcgatgaa tatttacatc atttgacaca taagcaataa atgaagcacg
    14101 attgccgctg ataaaatcaa atcttgcaag tccgcccaag aaaagtgttt gcccttcatt
    14161 cagctgataa actttaggtt tgatttcttt taacggtgca atgatccgca aatctttttt
    14221 acctaagtag tgcgccattt gatggcgatg gataattccc ggtgtatcaa tcaaaaaatg
    14281 cccgtcatct aaaggaatct cgattttatc caaggtcgtt cctggaaact gcgaagttgt
    14341 gattacatct tgtactcccg ctgtattttt gatgatttga ttgataagcg tcgattttcc
    14401 gacatttgtt actccgacga tatagacatc ccgtccatcg cggtattctt cgattttttc
    14461 taatagttcc gtcatctcat gagatttctt agcactagtt aataatacat ctactggacg
    14521 aattcctgct tcgtaagcac gttccttcat ccattgtgtt aatttagttt ttttcaatga
    14581 tttaggtaat atatccactt tgttcccaac taaaagtact ggattgtcac cgacaaaacg
    14641 gtgtagtcct gggattagtg aaccattgaa gtcaaaaata tccaccacat tgacgatcaa
    14701 tgcatctttt tgccctaatt cattcagtaa gcgcaaaaag tcgtcatccg tcaatgagac
    14761 atcttgggtc tcattataat ggcgtaatct gaaacaacgc tgacaataga cttccccgga
    14821 agctaaacct ttttccaaag ccgtttgcgg cgtgtatcct aattcaccgg gtttgtttgt
    14881 ttgtataacg gcaccacagc cgatacattg taattcttct gtcattcgat tccacctcgc
    14941 cagcccatat cagggtgttt tgctaataga taggccataa ttcttttttc ccaaaaacga
    15001 ttaaattgtg tcttccaact atctgttgcg ataatgggtt ttacaaggat gctacgtatc
    15061 cctgcacgat tggctccacg tatatcagtc ataatctgat cgcctaccat tactatctca
    15121 tcatctgcta aatccaattg tttctttgct atgttgatcc ctcttgctaa aggtttcatt
    15181 gctcgtgcaa catacaaaag atcaaatttt tcaatcgctc gcgcgacacg ttttgaatta
    15241 ttgtttgaaa caacaacaac agggattcct gcatttttca tttctaaaat ccaagtaagc
    15301 aactcttctg ttccatctgg attattccat gcaatcaacg tattgtctaa atcggtcaat
    15361 actgctttga tgcctagatt ctttaattgg gcaggtgtaa tctgatagat ggcttctacc
    15421 atccatgttg gtttgtaatt tgaaaacatg ctgtctcctc atctgcaaaa aatgaaggag
    15481 cacataaagt actcctcaac cccgtattat actgcatatc agcaaaaaaa actacctcta
    15541 tactcgttcg tctttcagaa aaagcatttc tttacaaaat gaccaaaaat aaagattgat
    15601 cacagaaaaa tttagaaaca aaaaacaact aatattggcg gaacctcttg tatcgttttt
    15661 ccagtaattt atgctgatct aatcgaacaa gttccgtcaa tctttcaatc aacgcttttt
    15721 tcataatgtg tgcgacttta tatcgtggca actgctcgcc gttgaacgtt tcaggaataa
    15781 ctcgatcaac gattcctaac tcgcgtagtt ctggcgcagt aattttcatc aactcagctg
    15841 cttctgcggc gcgactaccg tctttccaca aaatcgacgc aaatccttct ggagaaagca
    15901 cagcataaat cgtttcttcc agcatccaga cttcatccgc taaacctaga gcaagggcac
    15961 cgccacttcc accttcacca atgatgatgg cgatgatcgg tacctttaaa tcagacattt
    16021 cgagcagatt tctggcaata gcctctcctt cgccgcgctc ttctgctcct acaccgcaat
    16081 atgcacctgc tgtattgatc aaggtaatca ccggtctatt gaacttttcc gcttgtttca
    16141 tcagtcgtaa agcttttcga tagccttcag gatggggaga accaaaattt ctgcggatat
    16201 tttcctgcag atcccgtcct ttttgaatac cgatcactgt taccggtttg ccatctaatc
    16261 gagcgattcc accaacaatc gcttcgtcat ctgcaaataa acgatcacca tggcattcct
    16321 cgaaagaatc gaagatctct tcaaaaaaat ccaagcttgt ccatcgttct ttagctcttg
    16381 ctaactgaac gacatcatgt gctgtcttat ccacgatcta tccatccttt ctgtgtatgc
    16441 aattcaagaa gcgtatggat tttctgtttc aattccatgc ggggaacaat ctgatcgaca
    16501 aaaccatgag ataataagaa ttcagccttt tggaaatctt ctggaagttc ctgcttgatc
    16561 gtctgttcga tgacgcgtct tccagcaaaa ccgatcaagc tttggggttc tgctagaata
    16621 atatcgcctt ccattgcaaa actggcagtt actcctccgg tcgtcgggtc cgttaaaaca
    16681 gttagataaa aaagaccttc attgctatgc cgtttgacag cagcagagat ttttgccatc
    16741 tgcattagag aaaagattcc ttcttgcatc cgtgcgccac ctgaagctgt gaacaagacg
    16801 actggaagac tttcctttgt tgctcgttcg aataatctgg tgatcttttc tccgacaatc
    16861 gttcccatac ttcccatgat aaaattagga tccatcactc caataatgaa gggaatcccc
    16921 tgaatggttg ctttaccagt gaggactgct tcatgaagtc ctgttttatc ttgcattttt
    16981 ttaatttttt ccgtatattc gggaaatgct agcggatctt tggttacaag atccgtatcc
    17041 cattcttcaa aacttttttc atcaataatc aaagctagac gctgccatgc accgattcga
    17101 aaagcgtatc cgcaatgagg gcatactttt tcttcaccga tgtctttcgt ataaaggata
    17161 tgtttgcaat ttggacactt tgcccacatg ttatcaggaa cagatggcga gttagcaggt
    17221 ttgctctctc tattaggatt gatccgaata tatttctttt tcttaaacag agccatctat
    17281 gcgcctccta tctccgtttc tggtgtccaa ttcggtaaaa attcttcttg taaaaatgct
    17341 gtgctgtaat cacctgctat gactgcagga tgactaatca aatccatctg aaattctgcg
    17401 ttcgtgatga ttccttctgt gataaattca cttaaggctc gctgcatctt cattaatgct
    17461 tcaaaacgcg tatttccatg gacgattatt tttgcaatca ttgaatcata atatggcggg
    17521 atcgtatatc ccgggtaaac tgcactgtct actcgcagtc ccatcccacc tgctggaagc
    17581 agtaagtttt ggatcttccc tggagaagga gcaaaatgga aagcaggatt ttctgcatta
    17641 atccggcatt cgattgcgtg tccttggaat actacttctt cttgacacac attcaaaggc
    17701 tcaccggctg ctatctccac ctgttttttt actaaatcga ttcctgtgac catttctgtt
    17761 actggatgtt ccacttggat tcgagtattc atttccatga aataaaaatc gccttcttca
    17821 tccatcagaa actcgatcgt tccggcattt tcataattga ctgctttagc tgctcgaaca
    17881 gcagcgtctc ctagtgcttc tctttttgtt tgtgagatca ctacagaagg agattcttcc
    17941 agtacttttt gattgttgcg ttgtaaagaa caatctcgct cgcctaaatg aatgacatga
    18001 ccaaatcgat cacctaagat ctgaacttct atatggcgag caggataaat gattttttcc
    18061 aaatacatat catcatttcc aaatgccgct gctgcttctt gctgagctga agaaaaatgc
    18121 tgtgggagct cttcttttga ttgaactttc cgaatgcctt tcccgccacc acctgcagct
    18181 gctttcagca tcactggata accaattctg tctgctattt ccaatgcttc ttctactgta
    18241 gataatacac cagtacttcc tggaatgact gggacatttg cttcttgcat caattgacgc
    18301 gcattgatct tatttcccat tgcatcgatc gttgcacttt taggaccaat aaatgtgata
    18361 ttgcactctt cacacatgga ggcaaatcgg ctattttcag ataaaaaacc aaatcctgga
    18421 tgaattgctt cggcttttgt cacgattgca gcgctcagta cttcctgaac attcagatat
    18481 gaatcaacgg cttttgccgg gccaatgcag attgcttcat ctgctaactg tgtatgaagt
    18541 gcttcggcat ctgcttctga atatatcgct acggtttgga tccctagttc gcggcatgca
    18601 cgaatgatac gtacagcgat ttcaccgcgg ttagctatca atactttaga aaacatattt
    18661 cacctatcca atcataaagg ttaattccgc ttcggctact tttttaccgt ctactttagc
    18721 gattcctttg cctattccgg ctgaagcttt cactttcaat atctcaactt caaggtataa
    18781 ggtatctccc ggtgtaactt tcttacggaa ttttgctttg tccaatccgc cgaaataagc
    18841 cgtctttccc ttgaattgtt caagagaaag taatgcaact gctcctgctt gagccattgc
    18901 ttcaacaatc aatacccctg gcataacagg ttcttctgga aaatgtcctt gaaaaaaggg
    18961 ctcattgatc gttacatttt tcttagcaac gatccgttct ccttcaacca tttcttccac
    19021 tcgatcaatc aaaagcattg gataacgatg tggaatgatt tcttttattt cttgtatgtt
    19081 cattgttttc cctctttcct gctttgctta tttttgctga atcctaaaga gtggctgtcc
    19141 atattcgaca actgactcgt tttctacaag gatttctgtg atcactccgt cggcctcact
    19201 agttatctca ttcatcagtt tcatcgcttc aataatgcag ataacttctc ctttggcaac
    19261 ttgatcacct acttttttga aagctggttt gtcgggcgct ggttgcagat aaacgattcc
    19321 cacgattggc gaaacaacct cgattccatc aaatactgcc gattcttttt ctttcattat
    19381 ttctggagca gttgttgctt gttcaggtat gacattgact ggctccgaaa ctggcaacgt
    19441 ttcgtttgtt tgaaccatat tcatgcctcg acttgtttta tttttattca tatacaattc
    19501 aaattgacct tcacgcaaat cgaattccgt tagcgaggat tgatcaaatt gactgactaa
    19561 ttctctgatt tcattgatat ccaaaattat tcctcccagc gtttcatgca cagaaccgca
    19621 ttatgtccgc caaaccctaa tgaattactc attgcataag taaaacttgc aggtttgctt
    19681 tcgtttttga caactgtcaa gtcaatctca ggatcttgtt tttctacatt gatcgtaggt
    19741 gggataaatt gatgttctaa tgccagcaaa gttgccaagg cttcgatccc gccggctgct
    19801 cccaataaat gtccagtcat cgatttcgtg ctgctgacgt aagtatctct gaaattttca
    19861 cctaatgcga tttggattgc ttttgcttct gcctggtcat tggctggtgt acttgttccg
    19921 tgagcattga tatatccgac ttgttccggc tgtacaccag cttcttccat cgcaagcttc
    19981 attgctttag atgcaccaga accatctgga gtaggagctg tcatatggta agcgtcacag
    20041 tttgcaccgt atccaacgat ttctccatag attttggcac cgcgagcttg tgcatgttct
    20101 aaagattcaa gaactagaac gcctgctcct tcacccatca caaaaccatt ccgattttcg
    20161 tcaaacggaa tcgaaccacg attaggatca gttgattctg agagtgctgt taaagctgca
    20221 aatccagcaa tccccatctc acaaatcgtt gcttctgttc ctccagcaag aatgacatca
    20281 gaatagccat gtttgatgtt acggaatgct tccccaattg aattcgttcc gctagcacaa
    20341 gctgttacga tagctgtaca aatacctttt gcacctacac gcaaagcaat atttccagct
    20401 gccatgttgg caatcgccat tggtacgaac attggagaaa ctcgttttgc acctttttca
    20461 tgtagacgaa taacttggtt ttcgattgtt ggaagaccac cgatcccaga tccgatgatg
    20521 acgccaaacc ggtcttgatt cattttttct ttatctaaac cgctcatctc cattgcttct
    20581 aaagcagcgt agattccata gatcgaaaac atatccattc gtttgctatc ttttttaata
    20641 aaatatttat cgtaagggaa atctttgact tcacccgcta aggtaatccc tgtttctgaa
    20701 gcatcaaatt tagtgatcgg tgcgatgcca ttcgtcccct cttttaagct attcaaaaag
    20761 ctgtctgcgt catttccaat aggtgaagtg actccatagc cagtaattac cactcgattc
    20821 atttgtttgc tctccttttc ttatccgtgc atgaccaatc cgccatcgac attgatgact
    20881 tgtccggtga tatatggatt ttgtgctaag aaaactgctg tttgtgcaat atcttctact
    20941 tgtccgaaac gctgcatcgg aatttgcttt tccgcttgtt ccttgacttt gtctgctaaa
    21001 acttccgtca tatcagttgt gatgaatcct ggcgcaatgg cattacatgt aatgcctcta
    21061 gttgcagctt ctcgtgccac agattttgtt aatcctacta cgccagcttt actagctgca
    21121 taatttgctt gaccaatatt tccaatcaac ccagatacac tagagagatt aatgatggcc
    21181 ccttctcgct gcttcatcat ttttttcaat acgtgctgcg tcatattgaa ggttccaatc
    21241 aaattgatat ccagacattt tttgaaatct tctgcatcca ttcgcatcac aagtttgtca
    21301 tttgtgattc cggcattgtt tactaacaca tggatcgatc caagcttttc ttctgcttct
    21361 ttgatcattt gtcccgcttt ctcataatca gagatgtcac cggaaactcc tacgcatttt
    21421 acaccgaatg cttcgatttc ttctatcttt tcttttggaa tctctccgcg accattcaaa
    21481 atgatatttg ctccagcttt tgcaaaagct aaggccatcg ctttgccgat tcctcttgta
    21541 ctacctgtga taaatacatt ttttcctttt acatccataa cgttcctcct tatagtattg
    21601 ctattgtttc atcaaatgtc tgcttattct caacacgcag aactggtatt gttttatcga
    21661 tttttttcat aaatccgctt aatacttttc cggggccgac ttcgatgact tgttcgatgc
    21721 cgattgtttt taacttatgg atactctcat agaaacgaac aggttgcatg acttgctgtt
    21781 caagtagtcc cgcgattgtt tctttcttca ttatttccgt tgttgtgttg cttatgatag
    21841 gaaaagaagg ttcagagaat tgaatctgac ttaggtcctt cgccagtttt tttgctgccg
    21901 gttctaaaat agctgtatga aaaggcccgc tgacatttag agggatcatt cttttgaacc
    21961 ctttttcttt taatagtaaa acagcttcgt ctactgcttt ttcttcgcca ccaatcacga
    22021 tctgctgagg tgtattataa tttgcaggag atacgatgcc gtacttgcta gcttcatgac
    22081 agctttcctc gatcgtttca atcggcgcat tcatcactgc aaccattttc ccgcttccag
    22141 ctggggcagc ttctgtcata taagcgcctc tttttgctac taacgcaact gcttcagtga
    22201 aggatagcgc attactagct actaatgccg aatattctcc taaactcagt cctaaagctg
    22261 catctgggat gattccatgc tccttcaata agcgataata cgcgatgctg actgttagaa
    22321 tagctggctg tgtatattgc gtctgatcca aacgttcatt ttctgtaaag caaagttcag
    22381 ccatatcgta gccgagaatc tcacttgctt catcaaatgt ctgctttaca acggcttctt
    22441 catataaatc ttttcccata ccttgatatt gtgcaccttg gccactaaat aagaatgctg
    22501 ttttcatgtt tttctccaat atatccagaa tatatgtttg attcttccta ttcaaaaagg
    22561 ccgagacatt cctatttcag gaaatgtccc agcccccaac ctaattttaa tgattatgca
    22621 gtttgttttt ctacgtaagt cactaaatct tgaaccgttt tgattccgtc ttctgtttcg
    22681 attttcacgt caaattcatc ttcaatttca ttgatgattt ggaataaatc caagctgtca
    22741 gcttctaagt cttcttggat gtcagtagtc aatttgattt cttcagtttc ttttcctaat
    22801 tcttctgcga tgatttcttg aattttttcg aataccataa taatatattc ctccgatatt
    22861 gtttgctttt tatttttata atttaacagt ctacaactcc atgagaatac ttccccatgt
    22921 taaacctccg ccatagcctg taaatattac tttctgctgt tttcctaagg tgatctttcc
    22981 actagaaact gcttcatcca ataagatagg gatgcttgca gcagatgtat tcccatattt
    23041 atccatattg gtcaggaact tttcttgcgg catctttact tttcgggcaa ttttctcaat
    23101 aattctgagg tttgcctgat gcaaaagcag ataatcaact ggtttgtctt tcatgacttc
    23161 ttggatattg tttgcaacgt ctcgtacggc aaaatcaaaa atatctctgc cagtcatttg
    23221 caaataatac gaaccttctg aatcttctga atagaacgga tttttatttt gctggtagcc
    23281 agcggtcaat gagctacttc tggtaccgtc agctgctaat ttttctttca gcaatttttt
    23341 ctgtggattc gcttctaaga gaatcccacc tgctccatcg ccaaacaaca cagcagtcgt
    23401 acgatcagac caatcgatta gcttagatag tgtttcacca ccaatcacca gtcccttagt
    23461 tgagccgcag cggatcattt tttcagccat gcttaacgca taaacaaatc ctgagcacgc
    23521 agcactgata tcaaaagcga tagcatgagt tgctcccaat ttcccttgta cttgtgctgc
    23581 tacagatggt gtcgtgtaat cgggcgacat tgttgctact aaaataaaat ccaaatcttt
    23641 agggtttaga tttttctgtt ttatcaattt ttccgccact aaaatgcata aatcagaagt
    23701 ctcctgattt gttacacatc ttcgttcacg aatacccgtc cttgacgaaa tccattcatc
    23761 agaggtatcc atcatcttgg aaagatcatt attagtaata atcttttcag gaaggacgct
    23821 ggctgttgca gtaatcattc caaattgttc cattaaatat cctcacttat agtcttgcaa
    23881 gaagtcgtgc aagtttttca gtgcatccag caaggcatgc tgttcctccg tagccattcc
    23941 gtctaagatg ttcttgacca tttcacgatg aaaatgctga tggacccgaa atagtaactt
    24001 tccttttttc gttagtccca actttacaac gcggcggtca tcttcactgc ggatacgttc
    24061 gacatagcct ttttttacta acttgttaat agcagtggtc aaggtcccaa cagtaattga
    24121 tagttctttg gctacttctg aagttgtttt tttcttgtac atcccgattg cctcgatcgt
    24181 gtgcatctct gtgattgaca gatctttgaa ttgagatttt ttcagctcag actcttcgat
    24241 tgtcaaaata tcattgaaga cactaacaag ataatcattt actgttttta aatctggttc
    24301 catgttccac cgtccactaa ttagtttgat tatcaaatct tttgatgttc aaactatatc
    24361 ttaacttttt tcaaaatgc
//
LOCUS       DAMYXM010000035        23883 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.036, whole genome shotgun sequence.
ACCESSION   DAMYXM010000035 DAMYXM010000000
VERSION     DAMYXM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.036"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(53..787)
                     /locus_tag="PNS21_001619"
     CDS             complement(53..787)
                     /locus_tag="PNS21_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDI5159331.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="PNS21_001620"
     CDS             1114..1524
                     /locus_tag="PNS21_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDI5159332.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="PNS21_001621"
     CDS             1517..2218
                     /locus_tag="PNS21_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDI5159333.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="PNS21_001622"
     CDS             2218..3804
                     /locus_tag="PNS21_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5159334.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="PNS21_001623"
     CDS             3823..4044
                     /locus_tag="PNS21_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDI5159335.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="PNS21_001624"
     CDS             4041..4802
                     /locus_tag="PNS21_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HDI5159336.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="PNS21_001625"
     CDS             5182..5691
                     /locus_tag="PNS21_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDI5159337.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="PNS21_001626"
     CDS             complement(5766..6305)
                     /locus_tag="PNS21_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDI5159338.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="PNS21_001627"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="PNS21_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDI5159339.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="PNS21_001628"
     CDS             7318..8169
                     /locus_tag="PNS21_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDI5159340.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="PNS21_001629"
     CDS             8197..8832
                     /locus_tag="PNS21_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDI5159341.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="PNS21_001630"
     CDS             8852..9625
                     /locus_tag="PNS21_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDI5159342.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="PNS21_001631"
     CDS             10033..10830
                     /locus_tag="PNS21_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDI5159343.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="PNS21_001632"
     CDS             10808..11221
                     /locus_tag="PNS21_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDI5159344.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="PNS21_001633"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="PNS21_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDI5159345.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="PNS21_001634"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="PNS21_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDI5159346.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /gene="maf"
                     /locus_tag="PNS21_001635"
     CDS             15998..16558
                     /gene="maf"
                     /locus_tag="PNS21_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDI5159347.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="PNS21_001636"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="PNS21_001636"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDI5159348.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="PNS21_001637"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="PNS21_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDI5159349.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="PNS21_001638"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="PNS21_001638"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDI5159350.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="PNS21_001639"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="PNS21_001639"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDI5159351.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="PNS21_001640"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="PNS21_001640"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDI5159352.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="PNS21_001641"
     CDS             21905..22483
                     /locus_tag="PNS21_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI5159353.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="PNS21_001642"
     CDS             22535..23017
                     /locus_tag="PNS21_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDI5159354.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="PNS21_001643"
     CDS             23134..23508
                     /locus_tag="PNS21_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159355.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7808 a         4289 c         5125 g         6661 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttttt gagagtataa aatattttgt gtaaatgaaa aaatccatac aaaaaaggaa
    23881 gtc
//
LOCUS       DAMYXM010000036        23699 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.037, whole genome shotgun sequence.
ACCESSION   DAMYXM010000036 DAMYXM010000000
VERSION     DAMYXM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.037"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="PNS21_001644"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="PNS21_001644"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDI5159356.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="PNS21_001645"
     CDS             complement(1173..1868)
                     /locus_tag="PNS21_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDI5159357.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="PNS21_001646"
     CDS             complement(1881..2597)
                     /locus_tag="PNS21_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDI5159358.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3535
                     /locus_tag="PNS21_001647"
     CDS             2927..3535
                     /locus_tag="PNS21_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDI5159359.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3860..4183
                     /locus_tag="PNS21_001648"
     CDS             3860..4183
                     /locus_tag="PNS21_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDI5159360.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4365..5735
                     /locus_tag="PNS21_001649"
     CDS             4365..5735
                     /locus_tag="PNS21_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDI5159361.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5865..7166)
                     /locus_tag="PNS21_001650"
     CDS             complement(5865..7166)
                     /locus_tag="PNS21_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDI5159362.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7520..9478)
                     /locus_tag="PNS21_001651"
     CDS             complement(7520..9478)
                     /locus_tag="PNS21_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HDI5159363.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9655..9921)
                     /locus_tag="PNS21_001652"
     CDS             complement(9655..9921)
                     /locus_tag="PNS21_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDI5159364.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10431..12557)
                     /gene="pnp"
                     /locus_tag="PNS21_001653"
     CDS             complement(10431..12557)
                     /gene="pnp"
                     /locus_tag="PNS21_001653"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDI5159365.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12745..13014)
                     /gene="rpsO"
                     /locus_tag="PNS21_001654"
     CDS             complement(12745..13014)
                     /gene="rpsO"
                     /locus_tag="PNS21_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDI5159366.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13265..13828
                     /locus_tag="PNS21_001655"
     CDS             13265..13828
                     /locus_tag="PNS21_001655"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDI5159367.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14081..14602)
                     /locus_tag="PNS21_001656"
     CDS             complement(14081..14602)
                     /locus_tag="PNS21_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDI5159368.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14605..15447)
                     /locus_tag="PNS21_001657"
     CDS             complement(14605..15447)
                     /locus_tag="PNS21_001657"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDI5159369.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15440..16165)
                     /locus_tag="PNS21_001658"
     CDS             complement(15440..16165)
                     /locus_tag="PNS21_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDI5159370.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16341..16637
                     /locus_tag="PNS21_001659"
     CDS             16341..16637
                     /locus_tag="PNS21_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159371.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17205..18797
                     /locus_tag="PNS21_001660"
     CDS             17205..18797
                     /locus_tag="PNS21_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDI5159372.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18810..19550
                     /locus_tag="PNS21_001661"
     CDS             18810..19550
                     /locus_tag="PNS21_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDI5159373.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19718..21067
                     /locus_tag="PNS21_001662"
     CDS             19718..21067
                     /locus_tag="PNS21_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI5159374.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21195..21785)
                     /locus_tag="PNS21_001663"
     CDS             complement(21195..21785)
                     /locus_tag="PNS21_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDI5159375.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21932..23284
                     /gene="brnQ"
                     /locus_tag="PNS21_001664"
     CDS             21932..23284
                     /gene="brnQ"
                     /locus_tag="PNS21_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDI5159376.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6794 a         4772 c         4511 g         7622 t
ORIGIN      
        1 agggggtgcg acaaaagagc ttcttcaaaa aaataagtcg aaatctcaca caaattcgag
       61 aaataatttt cgtaagtttc ttcttattta ttgcatctga ccacttctgt tacaacctct
      121 cgttaaacgg tgttcaaaag cttgcatcta cgataataag cccaacccaa cgaaaaacgt
      181 gaggagctgt tttatttgga tgctcttatc gcttgatgca aagacagctt ttttacaacc
      241 tctacttcac ggtgctcaat cagctgacct tcggtattag ctcaaaaaac ggtaaaatat
      301 aaacagctat tttccgtttc ttctttttaa tacttaggtc aagatgctgt tttcacaacc
      361 tcttgttata aataaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcaaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 agaaagcaca ggtgaaggtg gcgaaccaga agtgatcggt tcaacagaag aataatactg
     3541 aaacaatcag gtggtgttag aagcggcggg tcagcagctt ttaacacgcc ttttttattt
     3601 ctaatcgttt attttcatat ggggcttcca cttttgtaaa tggctttaca aacgaaaaat
     3661 cctaatttac caagcttttc ctcgcatact tttatcgtac ctttataaaa atttatactt
     3721 tgaaaaaaag tattcaatta ttgttgcatt tttcctgatt ctttattaga atgaaccctc
     3781 gttgaagtaa gtatttctag tttatcctag gattaattat aaaaataaaa cattcgttga
     3841 ttcgaaggag gttaaacaga tgtttgaaag ttttgatagc agccgcaacc gttacgcttc
     3901 agttggtgtc gtatcaagtt tgccagatga attgattgac agtatatggt ttattattga
     3961 cctagatctt aagggagtca ttccattaga aaatatatta acctttgatt tgatcgaaaa
     4021 tcgtggaaaa gtgacgatgc atttttctca agcaggaagc aatgtggaaa tggatattga
     4081 tttgccgttt gcctattcga accaatttcc tgctcaagta tacgcctatg atgacggtac
     4141 gagagaaacg atccttttac caagtgagat tagacaatat taaggtgata gataaacgtg
     4201 gatgtccctt attttacaga ccaggccaag ttgttgattc tttgactcaa ttcttggctt
     4261 ctttttatgg agtttcataa attatcattg atttctaatt ggtattctca ggataaacgc
     4321 tatatactac taactaaatt ttaaagatat aaggagttta aaagatgcgc gatttaacaa
     4381 aaggaagccc cgctaaactg attttgatgt tcactgttcc tttactgatc ggaaatgttt
     4441 ttcagcagtt ctacaatatg atcgatatga tcatcgtggg tcagacgcta ggtaaaaatg
     4501 cgctggcagc ggttggtgca actggaagcc tgacattttt gatcattggc tttgcccaag
     4561 gtttaacagc tggtttagcc attattaccg cacaacgtta cggtgcaaaa gattatcggg
     4621 gtctgaagaa aagttttgct gctagtgttg tcattagtct tgtcgtcact gtgattttaa
     4681 ctgtcctaag tctcctgttt attcgtccga tgcttcaatt gatgcaaact cctcctgaaa
     4741 tacttgatca agcacaaaca tttatttcaa ttattttatt agggatcttt gcatcagtca
     4801 gtttcaattt gctttctaat gtcattcgcg cacttggtga cagccgcaca ccactattct
     4861 ttctgatcat tgcggtcatc atcaacgtcg tacttgattt gatctttatt atctattttg
     4921 gcatgggtgt ggaaggtgca gctattgcta cagtaatcgc acaagtttct tccagcgtac
     4981 tgtgtctagt gtatatcaag aaaaaaatgc ctttgttaca gttacgcaaa aaagatttca
     5041 aatttgaaaa agaagagttt gctgtacacc taaatgctgc tttgccaatg gcgtttcaat
     5101 cttcgatcat cgctgttggt gctatcgtgt tacaagcagc actaaatagt ctagggacag
     5161 atgtcgtagc tgctcaagcc gctgctggtc gtatcgacca atttgcaaat cagccgatga
     5221 tgtcatttgg tattgcaatg gcaactttca ctgcacaaaa ctatggtgcg aaagaatatg
     5281 gccgaatctt gcaaggggta cgtcaaactt tactgatgag tgtcggattt agtctcttag
     5341 caggtgctat cgtcattttc ttcggtcatt tctttgttca attgtttgtt agcccatcag
     5401 aaacacgtgt atttgaatta gcacaaacct acttcaatat caatgggtct ctttactgga
     5461 tcttagcagt cctgttcatt ttacgctaca cacttcaagg gcttggacaa agcaagatcc
     5521 ctacgatcgc cggtatgatg gaattgctta tgcgttcttt tgcagctatc gttcttacag
     5581 gaatgtgggg atacccagga gcagcagcag cttctccttt agcttggatc ggttctgttg
     5641 ccgtgttgct ttactcttat atgcgttcga tgaaacaatt gaaacacatg ggtgaagagg
     5701 aacatttcac attagaacat gcagaagcaa gatagccttg attaaaagaa atcgttgcgg
     5761 ctaaaaatga aaacttttat tgaaaatatc ttcttcgttt accgaaaaca ggttttttag
     5821 ataacaaaaa ggctgtgaca aaaaatgtca cagccttttt tcttttattc tgtttttctt
     5881 tccgtggact ctactttgaa ataatcgata tgtgcatgct ggtattctaa taccgtgaaa
     5941 cggaatgatg caatctcaat gattgttcct gcttttattt ctttatatcg gctagaagca
     6001 aagcccgcaa gtgtagtaaa attcgtttct tcaaactctg gaatctctac atgaaaataa
     6061 cgctggaaat catctaatgt tagtttgccg gagatgatat aagagccatc tgactgttcc
     6121 gctatcaatg cttcgtccaa atctgtctca tctcggattt ccccgacgat ttcttctaaa
     6181 atatcttcaa tcgtcactaa gccgcttgtc cctccgtatt catcttttaa gatagcaata
     6241 tgcttaccct tttttcgcat gatcgtcaat aattttttta tagggatcgt ttcagttgtt
     6301 acgatcggct cttcaatcaa ctgttctact gtcagctggt catctttata agattgcttg
     6361 atcaaatctg gcaaggtgac ataacctaaa acattgtctt ttgattctaa gataaccgga
     6421 tagcgagtat gtccttgttc aatcgaagct tttatcgctt ctttgacggt ttctgaagct
     6481 tcgaatactt gcatggacgt acggtctact tgtatatctt ttgccaaggt atcatcaaat
     6541 tcaaacacgt tttccaaata ataatattct tctttggtca actctccttt ttcataagaa
     6601 ttcaatgcga ctaatgtcag ctcttcttga gacaatgtct cttctccttc agatacgaga
     6661 cgcacaccta atagcttccc tagccctgca gctgaatgat tcaataccca aataaaagga
     6721 aacattactt tataaaaata atgaagtggt ttaactacga acaagacgac cttctccgta
     6781 ttgacgatac tatagctctt aggaagcagt tcgccaataa ccacatcgat atacgtaact
     6841 aaaataaatc ccagcgccac agagattgta ctgctaatag ccgaactaaa aggtagttta
     6901 ccgatcactg gttctagtaa agccgcgaca gatccttcac cgacccaccc gatgatcaaa
     6961 ccggttagcg taattcctaa ttgactagct gataaatagt tatctaaatg gtgtgtaaca
     7021 tgaagagcta gcttggcgtt ctttacattt tccttttcta gttgttccaa tcttgatcgt
     7081 ctgattttca ctaaagcaaa ttcacttgct acaaaaaatg aagtaattgc aatcatcaaa
     7141 aataaaataa tgaaattgat aaccatattc tcccgccttt ctctttttct attgtaccct
     7201 ttttctacaa aaagattgaa aataatgcgt attcatattt aggaataaaa aaggagatgc
     7261 ggcatttttc agccacatct ccttatcact tcaaaatcat tgcgttactt gttgctaaac
     7321 agctttttca tgacctctga taaaaacggt attcaaaagc ttgcatctac ggcattaagc
     7381 ccaaacagat gaaaaacatg agaagctgtt tttatctgct cgttcttact gcttgttgca
     7441 gtacagcttt ttcatgacct ctgataaacg gtgttcaagt agctgacatt cgacattagt
     7501 tcaggtcaaa gcgctacttt cacaaccgct ctttcaacca tgttttccga gtatttccaa
     7561 tgataagcgg aacagtttca tgttttactt cgctgtactc tagcccaaac catgtttctg
     7621 gcgaggtagc gatttttttg attgccagtt ttgcctgcat gatttgacgt tgccattttc
     7681 ctagagttgt cacattggaa actacttcat caatcaggac aaattggaaa tcgccaactt
     7741 ctcgccctgg tgtcagagag tatctttgtg gctgttttgg caaacggcct tgtttcatca
     7801 aatcatggat gacttggcga acatatacgt tgatttcctg ttggaccctg aaacctaaga
     7861 acagattcac ttggacaata aaatcagtgt ccatcatatc tacttgatat tctttcgtat
     7921 atggctcatc cgtcacttcc acatgaacaa accaatagac tcgtgctctt ttaggccgtt
     7981 tatctaaaat cgaatagatg aactgtcggc caactttatc ttcagccata tcaggaacca
     8041 agaagaccac atttgtctga tagattggta aagaagtatc atcacgtaat tctctaagct
     8101 gcgggatata atctctcaaa gacacatcct cggttgacga ttcgcgaata atgttccctt
     8161 gttcccaaat aaacatcacg actaagatga tcaaaccaat cagaacggct acaaaaccgc
     8221 ctcggaagaa tttgacaaca cttgagataa agaaaacgac ttctatcgca ccgaagaaaa
     8281 aggtaactaa atatgcaagc caacgagaat gcccattttg gataagataa aagtaaagca
     8341 gtgctgttgt catcaacatc gtcacagtga tcgatagacc gtaagcagct tccatatgat
     8401 gagacgttcg gaatgtgaca accactagag aacaggcgat ccataaaatc agattgactg
     8461 ctgggatata catttgcccg atactgttcc caggatacat gatctttaac cgaggcagca
     8521 gtttcaactt gattgcttct gaaactagcg taaacgaacc tgtaatcaat gcttgcgaag
     8581 caatgactgc agcgactgtt gcaaaagcaa cgccgatcac gatccaactt tgaggcatca
     8641 tttggaagaa tggattcagg ttcttgattt gctgtgcttc agggttttga taaacattca
     8701 acaaccacgc agcctgccca aaataattga taaccaaaca aattttgacg taaggccagc
     8761 tcagacgaat atttcgtttg ccgacatgtc caagatcgga ataaagcgcc tctgcacctg
     8821 tcgtagccag gaaaatattt ccaagaatga acaagcctaa tttgttatct gcactaaaaa
     8881 gcagctggat agcataataa ggattcagtg cacggataac tgtccagtct tgactgaagt
     8941 tcattaatcc cattacgcct aaaaaagtaa accaagcgaa catgattggt ccgaatgctt
     9001 ttccgacaat atctgtacca aagcgttgga ccatgaataa aacaaaaata ataactaaag
     9061 tgataatgac gatcacgttc tggttattac cgaaccggtc aaaaaactgc ggaattcctc
     9121 gcaagccttc aatcgcagtc gttaccgtca cagcaggggt caatacccca tctgctagta
     9181 aggcagctcc tccgatcatt gcaggaatga tcaaatattt cccacctttt cggacaagtg
     9241 tgtagagtga gaaaattcca ccttcaccat ggttatctgc attcaatgca atcacgacat
     9301 atttgattgt tgtcagtaga gtcaatgtcc aaaaaatcaa tgagactgat ccgataacaa
     9361 aatcaggatt gattcctgac aatccgccat tatcttcaac aatcgatttc ataacgtaaa
     9421 gcggacttgt cccgatgtct ccgtagacga ctcccatcgc tacaagcagg ccagccattg
     9481 atatcttttt ggtattgtgc gttgttgcca cgtttttccc tcatttcttg ttatatggat
     9541 atcctttagg taagcttgaa tgatagatga tattcttatc catgacaccc gctttcctaa
     9601 attgcttcta attatccaag taacacactg aagtgtcaac ttatttgaat aaaattattc
     9661 tttctttgga cgttctctga tacgtcttgc cacaaatgaa taaagagcaa ggcaaaaaat
     9721 tgcacccaat aatgctggta tcaaataaaa accgccgagt tccggtccca gtggtcctag
     9781 taaaaattcg ccgagccaac tacctaaaaa accgataatg atatttccag atacgcctcc
     9841 cgggacttcc tttcctaaaa ttgcgccagc tatcaaacct aatataccgc cgataaataa
     9901 cgaccataaa aaattcagca tctcctttcc ctcctttatc catatttctc acttttattt
     9961 tacgctttat tcagaaggac tccaaaaata tgcgccaaga aactccagaa aaaaagaagt
    10021 tgtaacaaaa gtgttcagct tatttccgta gaagctgctt ctggaacacc gtgaatcaga
    10081 ggttgtgaaa tcagcggttt gacctgagta ttagagaaac aaacggaaaa atagttcctc
    10141 atattttgcc gttttttgag ctaatatcga gggacagctg attgaacatc gtttattgga
    10201 gaggttgtaa aaaagctgtt ctgcatcaag taataagaac agccaaataa aaacagctcc
    10261 taatgttttt aacttggctg gacttattac cgcagatgca agcttttgaa caccgtttat
    10321 tcgaaaaaaa aggactgtga cacggctttt gtcacagtcc ccttttttcg aataattatt
    10381 gtccccctgt caccttcacg atcacaagta cattattcct atgaattaaa ttatttttct
    10441 tcatttttcg ttgaaccatc ttcgttcaac attgcttttc ttgacagatt tacgcgtcct
    10501 tgtttatcga tttcagttac tttcacaagg atctcatcac ctagtttaac aacatcttcg
    10561 actttattga cacgatcgtt tgctaattgg gaaatatgga caagtccatc ttttcctttg
    10621 atcaagttga cgaatgcacc gaatttttcg atacggacaa ctttccctag ataaacttca
    10681 ccaactttga cttctttggt caaatcttcg atgatcttga tagctttttt gatcatttct
    10741 gcatcagctg aagcaatact tacttttcca tcttgatcga tatcgatttt cacaccagtt
    10801 tcatcgatga ttccgttgat cgtatcgccg cctttaccga taacatcttt gattttagca
    10861 ggttcgatct ggatcatttc gatttttggt gcatattggc ttaattcttc acgtggagca
    10921 gcaatcgttg aagtcaattc ttctaagatt tccatacgtg cttgttttgc ttgagttaat
    10981 gcttcagtta ggatttgttc tgtgattcct tgaatcttga tatccatctg taaagcagtg
    11041 ataccatctt ttgtaccggc aactttaaag tccatatcgc caagatgatc ttccagccct
    11101 tggatatcag ttaggatcgt atagttttca ccatcagaaa caagtcccat cgcaataccg
    11161 gcgactggcg ctttgattgg tacacctgca tccatcaatg ctaatgtgcc agcacagata
    11221 cttgcttggg aagaagaacc atttgattcc aatacttctg ctactaaacg gatcgtataa
    11281 ggaaattctt cttctgaagg gattacttgt gctaacgcac gttcacctaa tgcgccatgt
    11341 ccgatttccc gacgccctgg agagcctgca cgtcctgtag aaccaacaga aaattgaggg
    11401 aaattgtagt ggtggatgaa tcgtttgctg acttccaccc ctaaaccatc aatgatttgg
    11461 tgctctccta gaggtgcgag tgtacaagct gataaagctt gtgtttgtcc tcgtgtgaac
    11521 aaacctgatc catgtacacg tggcaataag ctgacctctg atgacaatgg acggatttca
    11581 tccaatttac gcccgtcagg acggatctta tcgatggtga tcaattcacg tacgacatct
    11641 ttttcaagat cttctgtgat ttgtttaact tctttcaaca agtgatcttc atcttcatga
    11701 cctgcaaatt tctcagcata agcttcgcgg atatcgatct tcacctgctc gatattatct
    11761 tcgcgagcaa gtttttcttc agtcattact gctgctttca ttgtagcgta agaagcatca
    11821 aagatttctt ttttcaatac ttcatcaact tgcagtaaag tcacttccat tttttctttc
    11881 cctactgctt gaacgatttc ttcttggaaa gcaactaact ctttgatagc gtcaaaacca
    11941 aacagcaatg caccaagcat gtcttcttct gaaacttctt tcgcgccgct ttcaaccata
    12001 ttgatggctt gtttcgttcc ggcaacagtc aattcgatat ctgtttgttc tgcttgttct
    12061 actgttggat tcaagacgta ctcgccattt acacgaccaa cttctacgcc tgcgattggg
    12121 ccgtcaaatg ggatatctga gattgatagt gctaaagaag aacctagcat tgctgccata
    12181 gctggcgagc agtctgtttc tacactcatc acgatgttcg ttacttggac ttcattacgg
    12241 aagccttctg caaacatcgg gcgaattgga cggtcgatca aacgggcagt caatgttgct
    12301 tccgtacttg gacggccttc tcgtttgatg aaacctccag ggattttccc tgctgcatac
    12361 attttttctt cataattgat cgttaatggg aaaaagtctg tatcttttgc ttctttagag
    12421 gccacagctg cacttaagac tactgtatct ccataacgga ctaaaacggc tccgtttgct
    12481 tgttttgcta gctgaccgac ttcgatttgt aagggacggc cgccccatgt cgttttgaat
    12541 acttgttttt ctgacataac ttctcctttt ccttctcgtg aaagcaagac gctactaaac
    12601 aaatgaaaac tgccgaaagc cgtcttttga gcagctttca tttggttagt aggagtccat
    12661 ctagcttgat cacggaattt tacttaattg atatacgagt aaaaaaagtg agattcatgt
    12721 gaatcccact ctttttgact aaaattaacg acgcaagcct aaacgtttga tcaattcgcg
    12781 gtaacgttga acgtcagttt tacgtaagta agctaacaag ttacggcggt gaccaacttt
    12841 tttcattagg ccgcggtatg aatgatggtc ttttttgtgc acgcgtgcat gttcattcaa
    12901 gtggttgatt tcttcagtta atacagcgat ttgtacttct ggagaaccag tatccccttc
    12961 atggcgagcg tattcgttga tgatttcatt cttgcgttct tttgaaattg ccatttcttt
    13021 cacctctttc gattattccc ctgcgactga gtaaaacgtt ggtgattcgc ttaaccaagt
    13081 ggcaggtcat gtgcctagag cacacagcta tactttacct gaaaccgggt tgaaaatcaa
    13141 gtagttaatc attaagaaaa ggtagttttc aaacaatctt actactactt ttccattttt
    13201 gtggtaaact ataatgcgaa aatagacttg aaacacagtc ttttaagaaa ggggtacata
    13261 ttccatgatt acaatggatg atatcattcg tgaaggaaat ccgaccttac gcgaagttgc
    13321 aaaagaagtt tctcttccat taagcgaaga agatatctct ttaggaaaag agatgcttga
    13381 gttcttaaaa aacagccaag atccaatcaa agctgaagaa ctacacttac gaggcggtgt
    13441 tggcttagct gctcctcaat tagatatttc taaaagaatc attgctgttc acgtaccaag
    13501 tcctgaccca gaggcagatg gaccttctct aagtactgtt atgtataatc ctaaaatttt
    13561 gagccattct gttcaagatg cttgtttagg ggaaggtgaa ggctgtttat ctgttgatcg
    13621 agaagtacca ggatatgttg ttcgccatgc caaaatcact gtgtcttatt atgatatgaa
    13681 tggtgaaaaa cacaagattc gtttgaagaa ttatgaatcg atcgtcgttc aacatgaaat
    13741 cgaccatatc aacggtgtga tgttctatga ccacatcaac gatcaaaatc catttgcact
    13801 aaaagaaggc gtattggtga tcgagtagag aattggcagg ttccttcgat actatgctac
    13861 aagaaagctt tataaaaaag gggagtgctc aaaagctgca cttccctttt ttatgtttct
    13921 ccactaatcg aaatttagat agacatctag aaatcgcaat tccaaaaaat tctgtgtatt
    13981 ggatacgcat cttttcttat tttttcttat ttcttccata tgggtgttta tctcagagat
    14041 gatagaatag tttctaggat aaaaagtagc agctacgatg ttatccttcg tagaggattc
    14101 ctgcttcagc tagtttttgt ttgatgtttt tcagcatatg cttttctggg tagcggatcg
    14161 tatgcagatg gatgccgcct gttaattcgg ataacaaagt agcattactt ttttgataac
    14221 gtctcataaa ggaatcgata tcttgtggcg ttttgatgtg caaagttcct gctaactcac
    14281 cgtaaagggg atgttccaca attacatcaa tgatttcacc accattttca acgattagtc
    14341 tcagttcttc ttctgtttga tcttttccat gctggacagc gattttactg atgtgcccag
    14401 tattggtttc ttctgtttcg aggatataac ctcttgctgt tgccacgata cttttacctg
    14461 cagctcgtag taaagcaata tcgcctacaa tgatctgacg gctgactcca aaagttttcg
    14521 caaacttgct agcactcact ggtttttctg tatttgccaa ggtttgcatg atttgttttc
    14581 gccgtgtctc tccttcgatc acacttattc ctccttcatt ttgttttggt attcgcgcga
    14641 gtgtttcatc aaatcaacat acgtgtctgt tattgtttcc ttatatccct tatttttcag
    14701 cagactaaca actttatcca atacttgctg ctctcttttt gcatcataaa ctgcttggtt
    14761 ttcttctttt ttcacttgtc ctattttagt taccgtatcc attctttttt ctaataaagc
    14821 aaccaactct cgatcgatca tatcgatctt atgacgtaat gtttcaagag actcttctgt
    14881 tagttcttcc tctactgtct tgatgatacg agcaggattt cctgcaatca ctacattgtc
    14941 gggaaaagat ttggtcacaa ccgaacctgc gccgactact acgttgtccc ctagagtgac
    15001 acccggcgtg acgataactc ctccgcctaa ccaaacatta ttaccgattt taatgggttt
    15061 tgcgtactcc agaccgctgt tacgtttcac tggatgtagt ggatgcgtcg ccgtgtaaag
    15121 ctggacattc ggagcgaaca tgcagttatc accgatttca atcgtgctga catctaaaaa
    15181 tgtacaattg aaatttgcat aaaaattctc cccaacaaat atattatatc cgtaatcaaa
    15241 attgataact ggctccatat agattttttt ccctgtacgg ccaaaagtct ctttcaacaa
    15301 ttgggatcgc agttccgctg attctgcttg gttgatgatt tggctttgac ttcttgcaaa
    15361 catacggtcg gcaaccagct caggatcatt ggcaaaataa agctcgcctg caatcatttt
    15421 ttctttttcg gttttgaatt cagtcataat ctgctcctaa ttcttgtatt tttttgaaaa
    15481 caaccgctcc gtcttcttga ataaacgtat aaggctccgc taaataaaca ggcgtttgat
    15541 tgattactaa gattttggct gacggctggc gaaactgtac cagatcacaa aacggatgaa
    15601 cttgaaaact cgttccaaca atgacgatca gctctgcttg gctgacggca taagccgcct
    15661 gctccatgac tccatcttga aacccttcac catataacac gatgtctggg cgtatttgtc
    15721 ctccgcatgc ttgatgtttg tcgctgtgaa gatattcttt ccagtctacc gtttgttgac
    15781 attttcgaca atgacattga tacagactcc catgaaaatt gacgagttgc gggcttttag
    15841 cctttgcgtg caaaccgtcg atgttttggg aaacaaccca aacatttttt tctttcgcta
    15901 gatgtgctat ttccaaatga atgatattgg gttttgcttc aggatgataa atctttttga
    15961 caaattgata gaatttatcc ggttcattca ccattgcttg atgactcagc aagtattccg
    16021 gctgctccat cccgtggtag attcctgaca aagatcgata gtctggcact ccagaaggtg
    16081 tggatacccc tgcaccggtc aaaaatgtaa tttgctgata agctaatatc tcttttgctg
    16141 cttcgtctac tgttaattgt ttcatgtttt gtcacctcac tttccattat agcccatact
    16201 cgaacaaatt tattgttgaa aagtcagaaa aatgggaata aaaaagtgtt atgggcattt
    16261 gagcaattta tcacaatgtt cactgacaaa agaatgaaaa cgtgttacaa tactgctgta
    16321 taaaagtgag gtgaatcgat atgttaccat tctttattta ttggggcgtt attctggctt
    16381 gtatcgcctg gttagcgtta agcgtttatt tctctgtttt ttatcttgta cgcaaagaaa
    16441 atggaaatct ttgggctttt gctttattca acgtgattgc cgcaattgtt ttagccatta
    16501 cattagccgt atatcgaaca tggggctggg gaatcaccca atactcaagt ttgatttatc
    16561 taatattagc catttatggc gtcgttgttc ttttgcaagc gatcctagga cgtgagccaa
    16621 agaaagcggc agcttaaatg atcgagatgg aactggacac aggaaatttt ctgttctagt
    16681 tcttttttta ttgtattaca tacatttttg tgtataatta tactatcgtt tgcaatggat
    16741 agatgattca cattttgatt gttttttcgg taaaagtaaa gatttctata tttatcagta
    16801 ttttttcaaa aaccagcagt tatagattga caaagcctct cgagtatcgt aaactttttt
    16861 tgtataaata actgaatacg agatgcctca atcaatgagg tagaggtcgc gagaatcatt
    16921 aaccctgtgg agtgaagcaa acatggagaa gcaggaatag ggaggatcgc cgaaatgtgc
    16981 acttttttgc ttggagtgta tgttgggacg caagttaaga gcttgcggac tgtcttagca
    17041 gtgatgctaa gttgcgctat gttttgtgag aggtttatca ccttatggaa ttttagcggc
    17101 ctttttatat ctataaatcg gtcgcttttt attttttcag ataaagcttt accgcatttc
    17161 atctagaccg ctcgtgtatc atataatcag aaagggagaa aaaaatgaaa aaaaatatgg
    17221 tttcagttgc cgtactattt gcttttgtct tctcactatt cagtgtggga tctctggcaa
    17281 atgcagatga aaaaacaccg agcgatgagt ttcgcgtagg tatggaggct gggtatgcac
    17341 ccttcaactg gtctcaacaa acagatgaga ataatgcctt gccaattcaa ggccaaaata
    17401 gttacgccgg cggttatgat gttcaaatcg ctaaaaaagt agcagatggt ttagggaaaa
    17461 aattagtcat cgtccaaacg aaatgggatg gtctggctcc tgcccttcaa tcaggcaaga
    17521 tcgacgcgat catcgccgga atgagcccta ctgctgaacg acgaaaagaa attgatttta
    17581 ccgatcctta ttacgaatca caattagtag tagtagttca aaaagatagc aaatatgcaa
    17641 aagcaaaaag tttagcagat ctttctggag caaagatcac tgctcagtta aatacattcc
    17701 actatactgt gatcgatcag ataccagatg tgcagaaaca acaagcaatg gataatttct
    17761 cagcaatgag aacagcttta gcttctggaa tgatcgatgg ttatgtcagc gaacgtccgg
    17821 aaggtgtaac ggcaactagt gtgaacaaag acttggaaat gcttgaattc agcggtgcag
    17881 atggcttcca gacaaacccg gaagatgttc agatttctgt tggtatgcgt aaaggcgacc
    17941 cggatatcca aaaagtaaac cagattcttt ctggcatctc taacgatgaa cgaaccaaaa
    18001 ttatggacca agcagtcaaa gatcagcctg ctgcaactga tagtgaagaa aaacctggca
    18061 tcatttctga tttcactacg atttggaaac aatacggcaa tatgttctta cgaggtgctg
    18121 gacttacctt gttcatcgca ttgatcggga cagttgtcgg tactacactt ggattactga
    18181 ttggtgtctt ccgttcggta cctgaatcag gaaatgcact gactcgcttc ttccaaaaaa
    18241 tcggaaatgc acttctttct atctatgtcg aagtattccg cggaacaccg atgatggtac
    18301 aagcaatggt catcttctac ggtctggcac tcgcttttgg catttcattg aaccgaacga
    18361 ttgccgcttt gtttattgtc tctctcaata ctggagctta catgacagag atcgttcgtg
    18421 gggggatttt tgcagtagat gaagggcagt tcgaagctgc gcaagccatt ggtatgaccc
    18481 atggccaaac gatgagaaaa gttgttattc cacaagtttt acggaatatc ttgccagcaa
    18541 caggaaatga gtttgttatc aatatcaaag atacagcagt tttaagtgta atcggcgtag
    18601 ctgatttgtt cttccaagga aactctgctt ccggtgcgaa tttccaattc ttccaaacat
    18661 ttacgatcgt cgggatcatc tacttagtca tgacctttac gatcaccaga atcctgcggg
    18721 cggtagaaaa gaaaatcgat ggtccttcag cttataccaa gcttgaagaa atcgacaatt
    18781 caaatttgca ggaataagga ggaagaatca tgaatgaaaa aattattgaa atcaaacatc
    18841 taagaaaaag cttcggtgaa aatgaagtat tgaaagatat cagcgtaaca gtcaataaag
    18901 gtgaagttgt gacgatcatc ggttcttccg gttctggtaa atctactttc ttgcgctgta
    18961 tcaatttact ggaaaaacca acaggcggcg agatcattta taatggagaa aatgtattag
    19021 ctcctaaata taatttacct aaatatcgaa cgaatctagg tatggttttc caatccttca
    19081 acctgttcaa caatatgaat gtcttggaaa actgtatgtc tggtcaaaca actgtcctga
    19141 aacgagataa agaaactgcc aaaaaagtag caatcgaaaa tctggaaaaa gttggtatgg
    19201 atcgttatat cgaagccaaa ccagcacaat tatctggtgg acagaaacag cgtgtcgcaa
    19261 ttgctcgggc attatcaatg aatccagatg tattgctatt cgacgaacct acttcggcgt
    19321 tagaccctga aatggttggc gaagtgctta atacgatcaa agacttagca catactggct
    19381 tgacaatgct tatcgtgacg catgagatgg aatttgcaaa agatgtatct gaccgtgtca
    19441 tttttatgga taaaggtgtg attgcagaag aaggtacacc agaagatatc tttgtccatc
    19501 cgaaagaaga acgtacaaaa gaattccttt cacgtatttt aaatgcttag gatcaatagc
    19561 tgattgggac aaagagctgc ggtgtttacc gcagttcttt tttatgaatt actcataaaa
    19621 ggtaaatttc tgaaaatttt ggaatcttag ttgtgttttt cagaaaactc tcttaatatg
    19681 gaagtaatta aactttttta gttaggatga tttaaaaatg aatgaagagc aagaactgca
    19741 gcgtggactg aaaaatagac atgtccaact gatttctatt ggcggagcga ttggaacggg
    19801 gttgttttta ggttccggaa agtctatcca gctagctggc ccttcgatat tgctcgccta
    19861 tcttattact ggatgcatct gttttttcat tatgcgtgcg ttaggcgaac ttctgttatc
    19921 aaacaccaat aaccattctt ttctggattt tgttgcagaa tatcttggca aaaaagcggc
    19981 tttcatcact ggctggacgt attggttctg ctgggtatcg attgcaatgg ccgatttaac
    20041 agccatcgga atgtatgtac gctactgggc gcctggtgtt cctcagtggc ttcctgaact
    20101 gattgcatta ggtcttcttc tttgcttgaa tatggtggct gtctcattgt ttggtgagtt
    20161 agagttttgg tttgcattga tcaaagttgt agcgatcatt gcctttatcc tcgtaggagc
    20221 ttatatgatc ctcacccact atacgacaga tattggcaca gccagtatca cgaatctatg
    20281 gtctcatggc ggattctttc caactggtgc aaaaggattt atcttagctt tccaaatggt
    20341 aacctttgct tttgctggga tagaattagt tggattagtc gctggagaaa cagaaaatcc
    20401 tgaaaaagtg ttgccggaag ctatcaataa tattcctatc aggatcatct tattttactt
    20461 aggatctctt tttgtcatta tggcaatcta tccatggaac agcttgaatc cagataacag
    20521 tccatttgta gaagtatttt cggaaattgg gatcacgatt gctgcttcct tgatcaattt
    20581 agtcgtatta agtgcagcag cttctgcctg caacagtgca atatatagta caggacgtat
    20641 gcttcgttct ttagcgcaag aaggcagtgc ccctaaaaaa ttcaaaaagt taacaacaca
    20701 tcatgtgcca gggaatgctt tgacattttc tactatcgtc attttcattt ctgttatctt
    20761 gaattacgtg atgcctagtg aagtattcac actagtaagc agtattgcca ctacttgttt
    20821 cttgtttatc tggagtatgc ttgtctacac gcacatgaaa tatcgtaaaa gtatcctcgg
    20881 caaaaaagct cattcattta aaatgccgtt gtacccattt agtaattatc tggtatttct
    20941 ttacatggcc tttgtgtgtg tcgtcttgtt cctaggaaaa gatacgagaa tcgctttatt
    21001 actaacacct gtgtggttct tgcttctatt actaatctat catatgaaat atgaacgtaa
    21061 gaagtaagaa agtagttgtg acagaagtgg tcagcttcga gaaataagaa ggaatttacg
    21121 aaaattgctt cttaaatttt tgtgaaattt cggcttattt cttggagata aaagcttctg
    21181 tcccagcctc ttttctattt ccccggaaac aaccgctgat aataggcatc tgctttttta
    21241 taggaaaaaa tccaaatacc agatacaaaa aaacgtccga ataacccgat caacggtaca
    21301 aaaaagacac cggcaatcaa gcataagtta atcatcatgg agataagact tcgacggtcc
    21361 tttgacaaaa ctctcacgac atgggaatct ggtttatttg ctttcatgat ttgcctcatc
    21421 aagataccaa aagaaaaact ccacatcgtg tatactaaga cataagccat ctcaggccaa
    21481 aaagcttcag ggttacgccc gacccaacta gtaacagaag gacataaagt cagccaaaag
    21541 agcaaaaaga tattcgacca gaccactcgg taattaattc gttctgccat ctgaaatata
    21601 cgatgatggt tcacccagat caccgcaaca aatataaagc tgatggtata agctaataat
    21661 gtatttcgga tgacccataa agaatgaaag ctagatgtat tagggatctt gatctctaat
    21721 acaagaactc tcaaaacaat ggctaccact ccatcagtaa atgcttctaa tctcgacttt
    21781 gtcacgatga ttcctcctca gatgattcag cgcttttatt ttatcactta cgttattaaa
    21841 taaccatcgt tttatatagg gaattttctg aaaattgttg catatttttc gaaattttgt
    21901 tacttttaaa aaaaccgtaa aggagttatg tatgacaaaa aaattatcat ttaaagacta
    21961 tctttttata ggctccatgc ttttcggtct attttttggt gctggaaatc ttatctttcc
    22021 cgttcatttg ggtcaagaag caggcgctgc taccttttgg gcgaatctag gtttcttggt
    22081 taccggtatc ggtcttccct tcttaggggt gattgccatt ggtgtatcga aaagctcagg
    22141 tgtatatgaa ttagcaaaac ggataaatca aacatatgcg atggttttta ccatcttgct
    22201 ttacttagtt atcggaccgt tttttgcttt acctcgcctt gcaaccacat ctttcgagat
    22261 tggtttagca ccatttatcg atcctagcag acagacgcta tatctagcaa ttttcagtat
    22321 tgtatttttt gtgctggctt ggtggttttc aagaaaaccg acaaagatat tagattatgt
    22381 tggaaagttc ctgaatcctg catttttggt tttattaggt gctttgcttc tccttgcttt
    22441 tttgaatcca ctaggttctg taaatcaagc gcctattcag ccaaattatc aagagcatgc
    22501 ttttttcaca gggtttactc aaggatataa cacattagat gcgcttgctg ctttagcatt
    22561 tgggatcatc attgtcacaa caatccaaaa catgggtgta acaaaacctg ctgaaatcgc
    22621 taaagacacg atcaaatctg gtgcgatcag cattatattg atgggaatca tttatacttt
    22681 gctcgcttat ttaggtgcta tgagcttagg tagctttgct ctaagtgaaa atggcgggat
    22741 cacgctggct cagattgctg atcattattt aggtacttat ggcagtatct tattagcatt
    22801 tatcgttata ttagcttgct tgaagaccgg tattggtttg attacagctt ttgcagaaac
    22861 attcacagat ttgtttccgc aaagaaatta cgcattcttt gttgcacttg ctagtatcct
    22921 tccttgttta gcggcaaacg taggcttgac gaatatcatt caattttcgt tgcctgtttt
    22981 gatgttcatc tatccattag caatgacact gattttacta gtgttagttg gtcctttgtt
    23041 taagcagcgt acggctgttt atcgaatgac tacttatttt acattgattg cttcgatttt
    23101 tgatgggttg aacgcttgcc cagaaactat caaacaaaca cctattgttc aaaatctttt
    23161 atatgttgcg gaaagctact tgccgttttt caaattgggg atggggtgga tcgtaccagc
    23221 agttatcggt tttgttatcg gactgatttg gagtttcgct aagaaagaag aagtagcaga
    23281 ttgataaaag aggttgtgaa agagacgttc agccttgagc aataaaaagt aattttgaaa
    23341 aatggcttct cacatttttg cataaattta acttaatacc gagaaactga cttttgaaca
    23401 ccgtttattg agaggtcgtg aaaaagccgc ttagtttcaa gtattaagaa atattttgtg
    23461 aaaatggctt ctcacatttt tgcataaatt tgacttaata ccgagaaact ggcttttgaa
    23521 caccgtttat tgagcgtcta taaactgacc tgagtattag agaaacaaaa cggcaaagta
    23581 gctccatata ctttgccgtt ttgttttgaa ctaataccga agagaagctg atcaaacaac
    23641 atttctttta taatatagat tttagacata tatatgatat aaaaatagtt ttcgcaaaa
//
LOCUS       DAMYXM010000037        23167 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.038, whole genome shotgun sequence.
ACCESSION   DAMYXM010000037 DAMYXM010000000
VERSION     DAMYXM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.038"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..832)
                     /locus_tag="PNS21_001665"
     CDS             complement(<1..832)
                     /locus_tag="PNS21_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDI5159377.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     V"
     gene            1098..1442
                     /locus_tag="PNS21_001666"
     CDS             1098..1442
                     /locus_tag="PNS21_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDI5159378.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1443..1862
                     /locus_tag="PNS21_001667"
     CDS             1443..1862
                     /locus_tag="PNS21_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDI5159379.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1954..2304)
                     /locus_tag="PNS21_001668"
     CDS             complement(1954..2304)
                     /locus_tag="PNS21_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159380.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2270..3601)
                     /locus_tag="PNS21_001669"
     CDS             complement(2270..3601)
                     /locus_tag="PNS21_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HDI5159381.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3857..4423
                     /locus_tag="PNS21_001670"
     CDS             3857..4423
                     /locus_tag="PNS21_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI5159382.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            4568..4655
                     /locus_tag="PNS21_001671"
     tRNA            4568..4655
                     /locus_tag="PNS21_001671"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4600..4602,aa:Ser,seq:cga)
     gene            4989..5963
                     /gene="bsh"
                     /locus_tag="PNS21_001672"
     CDS             4989..5963
                     /gene="bsh"
                     /locus_tag="PNS21_001672"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDI5159383.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            6288..8183
                     /gene="asnB"
                     /locus_tag="PNS21_001673"
     CDS             6288..8183
                     /gene="asnB"
                     /locus_tag="PNS21_001673"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDI5159384.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(8378..8824)
                     /locus_tag="PNS21_001674"
     CDS             complement(8378..8824)
                     /locus_tag="PNS21_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDI5159385.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            9052..9201
                     /gene="rpmG"
                     /locus_tag="PNS21_001675"
     CDS             9052..9201
                     /gene="rpmG"
                     /locus_tag="PNS21_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDI5159386.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            9289..9807
                     /locus_tag="PNS21_001676"
     CDS             9289..9807
                     /locus_tag="PNS21_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDI5159387.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(9884..10840)
                     /locus_tag="PNS21_001677"
     CDS             complement(9884..10840)
                     /locus_tag="PNS21_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI5159388.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVSSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            11006..11812
                     /locus_tag="PNS21_001678"
     CDS             11006..11812
                     /locus_tag="PNS21_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5159389.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            11809..12798
                     /locus_tag="PNS21_001679"
     CDS             11809..12798
                     /locus_tag="PNS21_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDI5159390.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            12795..13799
                     /locus_tag="PNS21_001680"
     CDS             12795..13799
                     /locus_tag="PNS21_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDI5159391.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13931..14473
                     /locus_tag="PNS21_001681"
     CDS             13931..14473
                     /locus_tag="PNS21_001681"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDI5159392.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            14493..15191
                     /locus_tag="PNS21_001682"
     CDS             14493..15191
                     /locus_tag="PNS21_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDI5159393.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            15212..15424
                     /locus_tag="PNS21_001683"
     CDS             15212..15424
                     /locus_tag="PNS21_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDI5159394.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            15424..16386
                     /locus_tag="PNS21_001684"
     CDS             15424..16386
                     /locus_tag="PNS21_001684"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDI5159395.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            16404..16799
                     /locus_tag="PNS21_001685"
     CDS             16404..16799
                     /locus_tag="PNS21_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDI5159396.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16962..17327
                     /locus_tag="PNS21_001686"
     CDS             16962..17327
                     /locus_tag="PNS21_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HDI5159397.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            17324..19135
                     /locus_tag="PNS21_001687"
     CDS             17324..19135
                     /locus_tag="PNS21_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDI5159398.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(19197..19364)
                     /locus_tag="PNS21_001688"
     CDS             complement(19197..19364)
                     /locus_tag="PNS21_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HDI5159399.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            19612..20601
                     /gene="galE"
                     /locus_tag="PNS21_001689"
     CDS             19612..20601
                     /gene="galE"
                     /locus_tag="PNS21_001689"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDI5159400.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            20613..22097
                     /gene="galT"
                     /locus_tag="PNS21_001690"
     CDS             20613..22097
                     /gene="galT"
                     /locus_tag="PNS21_001690"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HDI5159401.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            22121..23101
                     /locus_tag="PNS21_001691"
     CDS             22121..23101
                     /locus_tag="PNS21_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI5159402.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         7479 a         3978 c         4792 g         6918 t
ORIGIN      
        1 ctactgaatt gaaatccata gatttcaata aaccatctct acgtaacaat ccgttagaat
       61 tctcgtttag gcctctttga gacggcgttc ctggatcagc gaaataaatg gcaatatcat
      121 tgacattact gatctgtttc caatttgaaa attcctttcc acaatcaaaa gtgatggatt
      181 tgaataggtt tttcggtaca gattcaaacc attgatttaa ttttttttca atatcaatcg
      241 cttgtctacc acacggtttc aatgtgatga taacttttga taatcgttca actaaggtaa
      301 ttacagcact tttatgtttc agacctacga tagtgtcacc ttcaaggtga ccaaactcat
      361 ttgagaattg gctataatcc ttttcacgtt catgaataga gcggcggaaa gtttgttttc
      421 cacgtctttc ttgatgtcca ttcggtttac gcttgccttt catcggtaag tcagaagaat
      481 caaatagccc ctttttgaac atacgataaa tggtacgagc agaacaacta ataggaaacg
      541 ctgcacgacc aacaatcaca tcgggtgtcc atccttgaac aacctttctt tgaatatatt
      601 ctgattgttc ctcgggtaaa acaagcggac gtctaccaca gtttgatttg tttttcttat
      661 actgttgata atattctaag gctgattttc cttgcttgaa gaacagatat actttatgga
      721 tcgtttgtct tgaacgattc aagcaatggg cagttttagc aacagattga ttcattttga
      781 aataagactc tattattaca agttcatccg ttgtaagatg ggtataggtc atttgtactc
      841 actccttata attctttggt cggaactatt ttgagtgtag cacaaatgac ttttttagtt
      901 gtctagctta attttacaat cggcgaataa aaaaagcgtt agcaactatg cttaaaaagc
      961 atatcgactg aataaactaa gcattggaca ggctgaaaga ctacatttaa aatgaaatgc
     1021 gaagaaacaa tagaaaataa agttgcctta gaaccatagt tgaatcacct aacagtcgtg
     1081 aagggagaat aagagaaatg gcagaaaaac aaaccgcagg acgagatcaa ttaggagatt
     1141 ttgcaccaaa atttgcagaa ttgaatgatg atgttttatt tggacaagta tggtccagag
     1201 aagaattatc tgctcatcag cgtagtatca tcaccatatc tgcattaatt tcaggaggaa
     1261 attttgaaca attgccagct catctgaaga ttggcaagca aaatggcatt actagtgaag
     1321 aaatagcaga aatcattacc catttagctt tttatgtagg atggccaaaa gcttggtcag
     1381 cttttagttt agcaaaagaa atctatttag aagaaaatag cgaagataag aaagaggcgt
     1441 aaatgacgac aaaacacgaa gaaatacaca atggcgtgat ttttccagca ggtgaaaaaa
     1501 acgaagctta cgctcaagta tttaccgggc aaagctatct gaatatgtta gtgaatgatc
     1561 ccgaagtttc tgttggtgta ggtaatgtaa cttttgaacc aggttgtcgc aatcactggc
     1621 atgtccataa agacggctat caaatcttac ttgtgaccgg aggagaaggc tggtatcagg
     1681 aagaaggaaa gccagcaaga cgattaatgg ctggagatgt cgtcgtaacg cacaaaggga
     1741 ttaaacattg gcatggtgcg acaaaagaca gttggtttga acatttggcg atcactgctg
     1801 gtaattcaga gtttttagaa cctgtaacag atgaagtgta tgaaggattg gaaaatcaat
     1861 aatgtcaagc ttgaacagct cctgaaagta gattttaatc gaactttcag gagctgcttt
     1921 tttgattcag tcttttataa atagaaaata gggttaataa taggaatgag aagcacgtcg
     1981 aaagatctta atattgataa tcggctgggc atacatgatt tccacgtcat caatcaaact
     2041 acctaacaga tgataatctt cccgatcatg tcggggatta cctaatagtt cttgataata
     2101 gatttccata ctagggtccc gaggattgtt cagcaaatca gcaagtacat ctaaatcttc
     2161 tggctgagtt tctgccgtac cctgtatctc tatgaaaagc ccttcctcag caaattctac
     2221 tccaatacgt atttcagtta tttcatggga atataaataa gtaagaagct catcgatgat
     2281 tctttttgat aattcttttt ccaagtgtct ttttttcctt tctgacagcg gtaaagttat
     2341 ctagtatcgg tacaaggata cctgcaacga aaccagttga aaaaccactg ttatatagat
     2401 ttagtcctcc gtgcaaagtg ctgacgttat ggacaagagc aatatgtagt acaccagcta
     2461 ttacaccgta aaaactgccg taaaaaccgc ttatgggcgc gagacctgtc ccaaacacag
     2521 ctgcaacaag catgccagta ctggtgatct catgaaaagt tccaaacaaa gaagcaataa
     2581 atattccgac tagtatcgga aaactgtttt ttaaatgaca accaaaagca ctgaaaccca
     2641 ccgcagataa aatagcaccg atgacaggtc catttaactg accgccaacc agcaaaacat
     2701 aagaagacag catcagacca actaagccta tgttgatcaa agtagcacca ataccagaat
     2761 tagcaatgaa atcagttgtc aattttccag atgaatcgaa aatttctcga atccccaaaa
     2821 gccgaaagga attataatag aaaccaattc cgactgtcag cgaaaaaaag ccaaaaataa
     2881 tccaaatgag aggaaagtga tattcattga aaaccaatgt attttcgacg attgaatagc
     2941 tgaataacct caaaacagca gtaaaagtca tcgcaatcaa cccagaggtg aatccaatat
     3001 tataaagtgt gaatccacga tggaaaacta aagtttgagc agctaagggc ggaagaagta
     3061 gaccaattgc tatgccagct aaattcccca ataaaatacc tatcagtaaa ggaaaacgca
     3121 taccaaaagt gatataacta acgaccggtg ataatgcgct accaaacagt ccgatcatga
     3181 tgtactgaga aaaaggttta ttgacaaata cagcgtatag atacacacct aaaatgattg
     3241 agatagaatt ataaaggttt ttgccaaaaa aagagaaacc ggtaactgtt agaatcgaag
     3301 caaataaggg accgtttaaa atcaccttgt gccgataagc taaaagcaca gaaagtaagg
     3361 tcaaaatccc tacatttatg aatgcgcttc cgaaaccgcc taaagcaaaa taatctgtca
     3421 aaagattgct tggcgaggta agaatagtta gcaatccact ccaattatca gtaggagtat
     3481 tagaaagcaa gccaataaaa agaaaaaaaa cacctgtaaa accaagataa aaatactttc
     3541 ttttctgtga aacgattcgt ttttgaggat gaatataaat aacgtcagtt tcgttttcca
     3601 ttacacacct tctttttcat tttctttaac tataacttga aatgaaagat ttggacaact
     3661 atcatttagg tcaaaactaa aaatgtaagg gaacccattg aatttaacca aattttttta
     3721 attttgcaaa aaaaagttaa agaagactga aaaaaaattt aaaatataac gttttgagta
     3781 aaatgtttgc aaaaaatgct aaaaataggg tacaataaaa caacatatac tgcagtaaag
     3841 aagtggggga gttttaatga gaagaaaagt ctataccaag gatcaaattc taaaagctgc
     3901 ttatgatgtt gttgcaaagg aagggtttaa aggatttact gcgagaaata ttgctaaaaa
     3961 gatggggatt tcgacacagc cgatttatct ggaattcaaa aacatggaag atttaaaaaa
     4021 tacgttgctg gattctatct ttgaacaact aaaagaagat gttttcccta ttgaacgtac
     4081 tggtgataaa atcattgatc tcggtttgaa ctatatctac tttgcaaaaa agcatcatag
     4141 tctttacatg gcattgtaca ttgatgaaca tggggacgga aagaaaatgc atgatttttc
     4201 atacaattat ttccgtgaaa tagttaaaga agatgaaaga tacaatcaat tatcggatga
     4261 atatgtcgat gctctgcata ctggtacatg ggtagcagtg actggcttag cgtcattgat
     4321 gtcatcaaac atcatgcatc caacagaaga acaaatcatt gcactgatca aacggacaat
     4381 tgactcgatt ttagcattag ataatcctga aataatcaga tagtgttcta tcagtatgaa
     4441 tttgatcggt aagtccgttg gctgcaaact gtcaatggac ttttttgcta aaaaaagcga
     4501 tttctgttat ttatctatta gaaataggct tgacatttcg tgaaaaaagt ttaaaaatag
     4561 tacttacgga aggttggcag agtggtaatg caccggactc gaaatccggc gaaccggcta
     4621 ataccggcgc gcaggttcaa atcctgtacc ttccttatat caacgattag aggttgtgaa
     4681 gaagctgtcc tgcatcaagt aataagaacg gccaaacaaa aatagctttt cctatttttt
     4741 cgtttgattg ggcttattac cgcagatgca agcttttgaa caccgtttgt tcggaataag
     4801 gggctgtgac aagacttttg tcacagcccc ttattgttgt acttttaacc tacaatattt
     4861 tttgattccc cctgagtact attttttata aatgctagta aatcaatctt ctttggcctt
     4921 tttttgataa aaaaaatact agatgtaaga taaaagtgta aaaacgattt gttttaggac
     4981 gggaaacgat gtgtaccgca atcacttatg caacgaaaga ccattatttt ggaaggaatt
     5041 ttgattatga gatgtcttat aatgaagtgg tcactattac tccaagaaat tatcgtttcg
     5101 attttagaaa ggtaaaaaat ttagataaac attatgcaat gataggaata gcagctgggg
     5161 tatccaacta tccactctat tatgaggcta caaatgaaaa agggttgagt atggcaggac
     5221 tgaattttcc tggcaatgct gattataaag aattacaaga aggaaaagat aacgttgcac
     5281 catttgagtt tattccatgg atactagggc aatgctcaac catagacgaa gctaaagaat
     5341 tattggctac tatcaatcta gtcaatatcg attttagtga aaaactaccc ttgtcacctt
     5401 tacactggtt attagctgat aaagaaaaat cgatagtaat tgaaagtatg aaagatggac
     5461 ttcatttata tgataatcct gtcggtgtgt tgacaaataa tccgccattt gattatcaat
     5521 tatttaatct gaacaattat cgctcgctat caaatggaac accagaaaat catttttcaa
     5581 accagatcag cttgaatgtt tatagtcgtg ggatgggagg attgggctta cctggagatc
     5641 tctcttctgt ttctcgattt gtaaaagcca catttactaa aatgaatgct gcatcaggtg
     5701 attcagaatc tgaaagtatc agtcaatttt ttcatatact aggctcagtc gagcaacaaa
     5761 aaggagtatg cgatactgga gaaagaaaat acgaatatac gatttattct tcttgctgta
     5821 acgttgataa agggatttat tattatcgta cgtatgaaga cagtcaaatt actgctgtaa
     5881 atatgaataa agaagacttg gatcgtcatg aattggtcag ttatccagtc ataaaagagc
     5941 aacagataaa ctatgtcaat taattaatga atagatccgt ttggtatcat attttctgat
     6001 acagtcataa ttagacacga tctttaggtc gtgtcttttt ttgctggttt ttttggaaat
     6061 agcatccact aatatttttt taacacgaag agaataaggt agcctcaaaa aagaaaacat
     6121 ggtaaattag atattgggac tttattttct gtaaaaacag aaactttcat cttttgtttt
     6181 cacgtgacga aattgtaaga gagaaaatat tcgtccgcta tttattctat gataagatgc
     6241 atatgaatga aataaacagt aattttatat taggatgtga aagttaaatg tgcggtattg
     6301 taggttttgt taacgacaag gacaacaaaa aaacaattat taatacaatg atggatcgaa
     6361 tcgttcaccg tggacctaat agttcaggag aatatatcga taaacatgtt gctttaggat
     6421 tcagaagatt aagtattatt gacttagagg gcggtactca gcctatttac aatgaagatc
     6481 ggacaaagat cattattttt aatggcgaaa tctataatta ccagcctttg agagaagaat
     6541 tgattgcagc tggccacgtt tttcaaacac atgcagatac agaagtcctt ttgcatggtt
     6601 acgaagaatg gggaacagaa ttattgcaaa agatccgcgg aatgtttgct tttgctattt
     6661 gggataatga aaagaatgag ttattcggtg caagagacca ctttgggatc aaaccatatt
     6721 attatgcaga aatgaacgga acatttatgt ttggttcaga aatcaaaagt tttctgcctc
     6781 atcctgattt taacaaagaa ttgaacaaag aagcgttgaa accttatatg actttccaat
     6841 attctccatt aaacggcgaa acattcttca aagatgtcta tcgtttgcct gaaggccatt
     6901 attatacgta taaagatgga aaaatagaca ttcagcaata ttgggatgca gattttgaaa
     6961 caaaagaaac gcattcccga caagaatgga tcgaaaaaat cgacgaaacc gttcaagctt
     7021 ctattgaagc acatacggtc agtgatgtag aagttggttc tttcttatcc agcggggttg
     7081 attccagtta tgtaacctct gtattgaaac cagatcattc tttctcaatc gggtttgatg
     7141 ataaaaccta taatgaagcg attgaagcca gaaaactaac cgaactattg gacttagata
     7201 atacagcagc agtgatcgac ggagacatgt cgttcaaagc attccctttg atccagtatc
     7261 acttagatga acctgattca aatccttctt gcgtaccttt gtacttttta gcaaatcttg
     7321 cttcacaaag tgtgcgggta gtccaatctg gagaaggggc ggatgaactg tttgcgggat
     7381 accaaacgta tggattccat acgaattcaa aattcatccg tgtgattgct caaggattga
     7441 aaaaattacc aaaaggaaca cgctataatt taggacgtaa aatcggaaaa atgaaaaatt
     7501 tccatggacg gatccattta tatgaatcac ttgctcctgc aaaagaatat tttatcggtc
     7561 atgctcgagt gttcgaagaa tcagaagcat cagaagtgtt aacaccaaaa tatcaaacag
     7621 caccatctgt ggatgaaatc atgactgttc attacgagaa aacagaagga atcaaagatg
     7681 aagtcaataa gatgcaatat gtcgatcttc atcaatggat gccaaaagat atcttgttaa
     7741 aggcagataa actttctatg gcaagttctt tggaagtccg agtaccttta ttggatatcg
     7801 aagtcatgaa attggctcaa caaataccaa gtaagttttt attgaatcaa aacaacacaa
     7861 aagatatttt cagacaagct gcgaacaaac atcttccgga agaatggtcg aatcgtgtga
     7921 aattaggatt ccctgtgcca atcaaagctt ggttgaaaga agaacacggc tacgaacaag
     7981 tcaaagcatt attcgaagca gattttgcaa aagaattttt tgatcaagaa aaaatcatga
     8041 agttgctaga tgaccatcac gaaggacgta aagaagagca aagaaaaatc tggacaatct
     8101 tcagtttctt gacatggtat aaagtctact ttgtagatga aacgattccg caagcagaag
     8161 caatcgacta tgtgactgtt tgatgaaaat caaatatcaa taaaaaagag gttttctcct
     8221 accgtttaac actgagtgat aagaaacaat ttcaaaaaac agctcttccc gttttataga
     8281 tattatgact tatggttcgg agttggcaga ggaacaccat tgtttcgaac ataagggggt
     8341 tgtgacacga ttttcgtcac aaccccttta catatgttca ataatcgatt tctttttctt
     8401 ttgtcccatc ttttttataa atgaacaaag ttgattcttt gttttgtgct acttgctttc
     8461 ctttttcgat tgcttctttt tttgtatcaa aatggttgct tgcctttttt gcccctttag
     8521 atttcacgat ccattgttct tcctcatatt ctacgatcac atctgaatca agcagttttc
     8581 cagcacgagg attcgtatcg tgttcatccg tcttgcttgg atttttttct ttttcgaaag
     8641 ttcgtttttc tgattcacta gcatcttcat accatttttt tgcttgatca atggcaattg
     8701 ggatcgcacg gttatcaggg tacccttcat ctaataatgc atttgcgatg tcgattgctt
     8761 tttttcttgt caatttatct aagtttttca ttgatgcagg aaagtccttc atgttccatg
     8821 gcatattagt atcccccttt attaaaaaac ataatctata taaacaagtg taagggtatt
     8881 tatacaaaaa gaaaaagatt ttgtttgaga agtaaaaccg ttgtatttat cgtcaagtag
     8941 ttttacttaa caaaatcgtg aaaaacacta gcaatctaga aaaaatcatg gtaagataat
     9001 caagtctgag agtcatctta gaggattctt gtaaaaatgg gagggaaaaa catgcgcgta
     9061 aacatcactt tagagtgtac ttcttgtaaa gaacgtaact atctaacaag caaaaacaaa
     9121 cgtaacaatc ctgatcgttt ggaaaaacaa aaatattgcc cacgcgaacg caaagttact
     9181 ttgcaccgtg aaacaaaata atgtttttaa agttctcaat tccttgctgg tcaaagaatt
     9241 gagacttttt ttatctcaaa acatctaaaa tcataaggag cggataatat gtttatttcc
     9301 atgtgggcac aagataaaaa tggtctgatc ggcaaaaatg gtttgttacc atggcgattg
     9361 ccaaatgata tgcgtttctt tcgcgaacac acaatggatc gaattttagt gatgggaaga
     9421 aagacctatg aaggaatggg ggatctttca cttccttatc gccatatcat cgtgttgaca
     9481 acgcaaccaa attttcgcac gaaagaaaat gcggaagtaa tgcattcgat cgatgaactt
     9541 ttgtcgtata gtcaaacgat ctcagaagat atctatgttt cgggtggtag tcggattttt
     9601 caagaacttc ttccttacac tggtcggatc tggcgaacat tgatcgatag cacgtttgaa
     9661 ggcgatacgt atattgggaa tattgatttt tcagacttcg cccttataga ggagtatgag
     9721 gggataacgg atcaagaaaa tctatatgcc catcgttttc aaaaatggga aagagtaaaa
     9781 aatagggttg cttcaaaaga agaataaaat ctatgcgtct agtttgttaa taagaaaaaa
     9841 agcatctcac gactaatcgt gaagatgctt tttttagtta aaatcaattt agtttttctt
     9901 tgatatcatt gaccatttcc tgactggcaa taggtccgtt atatagccaa ctagattcag
     9961 gtccgaattg cagtacatga ttgtctgtca ctgcttttag attcttccag cttggttcat
    10021 tgaacatggc agcatctttg tcactgctca caaggatgat gtagtcagca tctaattcag
    10081 ctagcttttc agaggaaaca gcagaccagt cagaagtggc ttttttggag acctcttcca
    10141 ctagatcagg aacaccaaat tccagatcgt cgtatacgat tcttccactt gaccgatttt
    10201 tgctgaccat aaaggctgag ttgttggtca cccacaacac tgcagctgtc ttgttctcga
    10261 ttttatctgc tagttcttct cgagttttct tgatggattt ttgataatcg gaaatgattt
    10321 tttctgcctc tttttctttt gccaaagctt tgccgacatc ttttagttgt tcttcccatg
    10381 tgacatctgt tccgtttttt acaacataag ttggcgcgat tttgctatat tgttcgtatt
    10441 tcccgccttc gacagtagcg gaagaagaaa tcaacagcaa atcaggctca aagcttaaca
    10501 ctttctcata aggaaggtca taagaaatcg ttgggacatc ctttaaatca tcttgcagat
    10561 aatcttgaat ggaaccactg ccaacagtcc actgggcgat aggtttttcc cctagtgcaa
    10621 tcagatagtc ttctaagtaa ctgccgatga tccgtttggg cgcgtttgga atctctactt
    10681 tatgtccaag tgtatctgtg actgtatgag tactattgga tgaagtcgtc gaattggcag
    10741 ttgtagctgt ttctttatct gaagaggggg agcaagcgct cagtactcca gccagcagca
    10801 atatgctaga taatgtgaat acttttgttt ttaatttcat ttctttcctc ctaacataat
    10861 tgagaatctt tctcattggt tattattcct gatatttcga acgctgtcaa ccgcttcatt
    10921 tggctataga tatgaaagta gcgcttgacg aaatgttgaa acagcgtcat aattgagaat
    10981 gattttcagc gaaaggatgg gtgaaatgac gagattaaga gcagctgaca ttgcagccgg
    11041 ctacgaaaac aaaaaaatca ttgaaaacat gtctgttacg attcctgaag ggaaaatcac
    11101 tagtttgatt gggccaaatg gaagtggaaa atctacttta ttgaaaagtt ttacccgtat
    11161 actacctgtg aaatcaggaa agatcattgt caataactct gatatctcta catacagttc
    11221 aaaagaactt gcaaaaaaaa ttgcattatt agcacaaact agtgaacacc ctacaggtat
    11281 gacagtagaa gaagtagtat cttacggacg atacccttat caaaaactgt tcagtggcat
    11341 ggacacgcaa gatcatgaag caattgagtg ggcgttggat gctacaggtc tagctggatt
    11401 tcgtatgcgg gatatcgcaa cgctgtctgg tggacaaacc cagcgtgtct ggatcgctat
    11461 ggcgcttgct caggaaacag atatcttgat cttggatgaa ccgacaacat ttttagatcc
    11521 ggcacaccaa ttagagatcc tacagttatt gactgaaatc aatcaaatga aacaaacaac
    11581 gatcttgatg tcgattcatg acattaatca tgcttcacgt ttttcagatt atttattagc
    11641 aatgaaaaac ggtaagttga tcacagaagg tacacctgaa gaagtgatca caaaagagtg
    11701 tatgaaaaaa atatttgaga ttgatgcaac gattgtcacg cttccaagaa caaataaacc
    11761 agtttttcta acctatgatt tgttgccaaa agagcaggag gatcaattat gaggactatg
    11821 aagaaaaata gactcctgct tttggtctgt tttgttcttt tgattatttt ttttgttgct
    11881 ctttcaaatg gcgcagcgac tgtgggaaaa gcagattttt ggcaggcgtt gtttgatttt
    11941 gatagatcaa aacaaagtca ccaaatcata cgaaatattc gtttgcctcg tgtggcggga
    12001 gcatttcttg tagggagttg ttttgcattg agcggtgcat tgatgcaagg agtgacaaga
    12061 aatcctttag cagattcagg cttactagga atcaacgcgg gtgcttcgtt ggcaatggcc
    12121 cttagttttg ctttttggcc taaagcttct gatttttctg tgtttctaat gtcattggca
    12181 ggttcttttg ctgtaacttt gctcgtattt ggttttttgc agttttcgaa aatgggcatg
    12241 aatcctttac agctgatttt agctggcgta gctattagtt ctttttttac tgctatcagc
    12301 caagcactta cccaattctt taatctgcag caggatcttg ctttttggtt tgtaggcgga
    12361 gctgccaata tcacatggaa acaattagca gtattgtttc ctttatatat acttgctgtt
    12421 ttggtttctt tttcattagg aaaagcaata tctttgattc atttgagtga aacacatgca
    12481 ttagctcttg gaagtcatcc taaacgaacg cggttgattg ttttctgtct agttgcagtg
    12541 ttagcagcca ttgccgtttc gttagtcgga cctgtttcat ttattgggct gttggttccg
    12601 ccagttgttc gcggaatggt tggaacggac tatcgttatg ttctacctgt ctctttttta
    12661 gttggcggtg gattggttat gttggcggat tttacagcga ggatgatcaa tccccctttt
    12721 gaaacacctt ttggattagt cattgcattg attggcgttc cgtttttact gatacaagta
    12781 aggagggaac aacggtgaaa aaaatatatt ggacgttcta tttcttgctt tttgtacttg
    12841 tcatcctgtc tataggtagt ttaatgattg gtacgccttt tatttacctg gatcagcttt
    12901 ttgccacgtt catagggaaa gaatcatctt ctttggcgct gattgttaga gaatttcgag
    12961 ttcctcgatt actgatcagt ttattagcag gagcttgtct tggcgtttct ggttatatct
    13021 tgcaaggagt aacaagaaat gaattggcag attctagtat attgggcatt aatgccggag
    13081 caggattttt agtgatgctt tatttaggtt tcttttcgca ctattcgctg ccgtttctat
    13141 tgctagttgt agcctttcta ggtggtgttt tagctgcttg ctgtgtctat cttgcggcct
    13201 atgatcgaaa tggctttttg ggtatgaatc gaatcttatt gtccggtatt gccgttaatg
    13261 ccggcctttc tgcattaaca ttgctatgca cgatccaatt atctaaggaa aattatggat
    13321 ttgtgaatgc atggttagcg ggatcaatct ggggtgcgag ctggccttat gtttgggcat
    13381 tgcttccatg ggcggttatc ttaattccgc taggaggact ttatgcacag aggattgtat
    13441 tgctatcatt tggtcaagaa cgcgcgcaag gattaggcct taacgtggct aaaagtcaaa
    13501 aaatcctttt gttgttagct gttgctttgg caagcggtag tgtagcggtg acaggtagtt
    13561 tatcttttgt aggactacta gctcctcatg ctgcgaagct agtcgtacgt aaagaaaata
    13621 actggacctt tgttctgagc ggtttgttcg gttccatttt tctgcttagt gcagatatgg
    13681 ttgctagagt gattctacta agtggggaag tacctacagg aattttgatt gccctttttg
    13741 gtgcgccata ttttctgtat gcacttttta tcagacaaaa acacgcgctg aacagttgaa
    13801 attcattaaa aaaggaaggc caatcatttc taggtcttct ttttattcta tttatgcatt
    13861 tatttgaaag ttcgattgct atttttcttt ttttcctata ccattttatg agagaaggaa
    13921 gggtgagttt ttggaccaat tgaaagtaag attacgccgg aaaggaatcg aaaagctgaa
    13981 aaatctggcc aaaagtggca agaaaaagga aaaagaacag cgaatctata aacagctatt
    14041 cgccagcgag tactggaagt cagcaaaagt gatagggacg actttatcga atgaatttga
    14101 actggataca cagccggtta tccaacgagc agaaaaagaa caaaagaaaa tagttgtacc
    14161 taaaactttg ccgaaacgtc agatggcgtt ttatgaaatg gatgaacagt ccgttcttgt
    14221 acgttcgagt tttggtgttc ttgagccaca atcagagaag ctctatttgc cggagatgat
    14281 cgatctcctc attgtaccag gtattatcta caatagagcc ggttatcgaa tcggttttgg
    14341 cggtggttat tacgaccggt atttaaataa ttttcataaa aaaacatgta gccttgtttt
    14401 tcgtgaacaa ttgaatgaac aatggcaacc ggagagcttt gatcagaaga tccaacaatt
    14461 gtttattgac taaaatgaaa tgggaggttt tcatgaatta tcaacagcaa ataaaattgc
    14521 gtgcatggtt aagacgaccg ttatggacct atgctttttt agggatacag acagcggtct
    14581 tcctcatgat ggaattattt ccgcgacttg agatccctta ttatgccgga atgtatggtc
    14641 cttatcttgt tcacttcaat gaatggtggc gtttagtgac accaatcttc atccattttg
    14701 gactgatgca tttcgtaatg aactcactga tactgtattt tatggggcag caaatcgagg
    14761 caatctatgg ccattggcgt tttttcttga tttatatgtt cagcgggata atgggaaata
    14821 cagcaagctt tgcttttaac gaagcgaatg ttctgtctgg tggtgcaagt acttcgatat
    14881 tcggtttgtt cggtgcgttg tttatcttag ggtttcattt taaacataac ccagcgatcc
    14941 agcagctagt cagacatttt cttttattta ttgtgttgac atttgttttt ggtcttctgg
    15001 atacctctgt agatgtttgg ggacatgtcg gtgggctaat tggcggatta gtgttaggga
    15061 atgttttggg tctaccgaaa caacagacaa gctattcgat acaccaaagg attctttcta
    15121 cactagtttt tgtatttctt tttgtaatat gtattttact gggtttaaaa aaatatggtt
    15181 tacttgtata atatgaagga agtgcttaaa gatggagagc ttatatgatg tacagcagct
    15241 gttcaaaaaa tttggcatct ttatctatgt gggtgcacgc atctatgaca ttgaattaat
    15301 gactatcgaa ttgcgtaatc tgtatgatag ccgtttgatt gatcatgata catacatgac
    15361 agcgtggcgc atattaaaaa gagagcaccg aattgaagaa agccgaaaga aaggaaattt
    15421 gtaatggaca agaagattat tgggattgat ttaggtggaa caacagcaaa atttgcaatt
    15481 ttaacaccag aaggagagat tcagcaaaaa tggagcatcg ataccaatat tttggatgat
    15541 ggtaagcata tcgttcctga aatcattgaa tcaatcaatc atcgtttgaa tttatataat
    15601 atgaaagccg aagactttat cggaatcggc atgggtaccc caggaagcgt ggacagcgaa
    15661 gctggaacgg ttattggtgc gtataactta aactggacag aagttcaatt tgtgaaaaaa
    15721 ctgatcgagg caggaacagg aatcaaattc gctatcgata atgacgcgaa tgttgctgct
    15781 ttaggtgaac gatggaaagg tgctggagaa aacgatccag acgttgtatt cgttactcta
    15841 ggaacaggtg ttggcggagg tatcgttgct ggcggaaatt tgattcacgg cgtgagcgga
    15901 gctggaggag aaatcggaca tatcactgtg gatcctgaag ggttcgaatg tacttgcggt
    15961 aaacgcggat gtttagaaac tgtatctagc gctacaggtg tggtacgcct aggtcgtttc
    16021 ttggcagaag aatacgcagg ggattcaaaa ctgaaagcaa tgctagacaa cggagaagaa
    16081 gtaacaagta aagatatctt tgaaatggct caagaagacg atccgtttgc attgatggtt
    16141 gttgatcgcg tatgcttcta cttaggttta gcttgcggaa acttggggaa tacattaaat
    16201 ccgtcaagta tcgtgttagg tggaggagta tcggcagcag gagaattttt acgtagccgt
    16261 gtagagaaat attttaaaga atttactttc ccgcaagtaa gagaatcaac aaaaatcaaa
    16321 ttagcagagc ttggtaatga agctggtgtc attggtgctg cctcattagc cttgaaattt
    16381 gcttaaaaaa acggaggcat tacatggttt tatgggtaat taatatcatt ttgctattga
    16441 ttgtgcttgc aatcatcctt tgggaagtct atctacgagt aatggccaaa cgttcagcaa
    16501 cgactttgac agaagaagaa tttcgagaag gaatgagaaa agcacaagta atcgatgttc
    16561 gcgaaaaaga tgtgtttgat gctgggcata ttttaggtgc aagaaacatc ccttacactg
    16621 ttttgaaaga ttcattagga tctattcgaa aagaccagcc tgtctatatt tacgatcaaa
    16681 agaaaagttt gagcatccgt acagcaaata aattacgtaa agcaggttat caagagattt
    16741 atttacttaa gggtggatat gacggatggt ctggaaaaat caaaagtaaa aaagtataaa
    16801 acagatgatt gtatgacagc tttaaaaacc gaatcaatca ttagattttg accaagatca
    16861 catgaacggc gcaatggata gttgtgccgt tcattttttt tattttgtgc tattatctac
    16921 atatataagc taagcgaaaa ggtagaaaga aggaaacaac tatgatcaaa ttaaaacgtg
    16981 tttaccttga tgctgaaaaa gaggatggct atagaatact cgtggataga ttctggccac
    17041 gaggaatttc aaaagaaaaa gcgcagttag atctgtggtt gaaagaaatt gcacctagta
    17101 atgaagcacg caaaactttt gaccatgtac cagaacgatt tgcttcattt gcaaagaaat
    17161 accagcaaga attgattttc gaagaagaaa agaagcaagc tatttttaaa ctcagtcaac
    17221 ttatgaaaga acatccagtc attacttttg tttatggtgc aaaaaatgaa catcagaacc
    17281 atgctgtttt attgaaaaaa tttatcgaaa tggaggcatg caaatgaaaa tagggatcat
    17341 tggtgccgga cctagaggat taagtgtagc agaaaggatt ctccataata atcaggacag
    17401 acattctctt gaactttttc ttttcgatcc caatggtcct ggaggtcgag tttggcggtt
    17461 gaatcagcca ccggagttat taatgaattc tgtttctcaa caagtgacgc tttttacaga
    17521 tgaaacattg tccagtggag gattggtaac acagggaccc aacttgtttc aatggagtaa
    17581 aggacctgct acagattata tcgaacagac ggtaaccaaa aataagcaac taattataga
    17641 agaaatcaat cgtctggaaa aagatcagca atctactcga tgtttatacg gactctatca
    17701 acgctggttt ttctcaaagt tgcaagaaga atttccagct tctattcagt ttgtttattc
    17761 tttagtaaaa aaagtaagca aaagaaaaga tggttatctt ttacagacag atcagcaaag
    17821 ctattctatc gatcagcttg ttttagcaag cggtcactgg gaaaatgatg agacagaaga
    17881 agaaagagtg tgggtagact acgcaaagaa aaatgtttta ctgtatcagc ttccttctaa
    17941 tcctgcagat gtagatcttt cgaagatacc gcctaaagaa aatgtctttt taagaggtct
    18001 aggcttaagt ttttttgatt atgtcggatt gtttacagta tcaagaggag ggcgcttcga
    18061 gaacgaaaag ggaaaactgg tctatcatcc atcaggcaag gaaccgattg tatacagtgg
    18121 atcaagaagg gggctccctt attaccctcg aggaagaaat caaaagcaag gcggagcaat
    18181 ggcatggcca cggttactaa caaaagaaaa cctggagcaa tggcatcagt cgggattttt
    18241 gactggcgaa acattttttg actatctgaa aaaagacgtt gagttgtttt attacaagaa
    18301 aatcatcgaa gaatatcact tgccgattcc aaagaaaaat tttgagcatg attttctctc
    18361 cctctcttca gaggagtgtc tcggaaagca tccagcacta atcccttata gatgggattg
    18421 gtcgtattta gaaactcctc ttgcctatca gaatgagccc ttttcagaag ctagccgaat
    18481 atttattgaa gggcagataa aagaagcgga aaaaggcaat tgcacaggag catttgcttc
    18541 aacatttgat gcattaaaag actggcgtga tccaatccgg caggctattg aatggcacgt
    18601 tttttctgct aaggaataca aagaaatact atggggatgg tttactcatc taaatagctt
    18661 tttaacgatc ggtccgccaa tcattcggac gaaagaatta gcagcactga tagatgcggg
    18721 tatttttcat ttgatcgaac cgccatttga tattcagcaa aagcacaacc gctatgttat
    18781 ttctctacaa gataaagaaa tcagcagtcg ctatttgatt gaagctcgat tgccacaaac
    18841 aagcttttct cgttcaagta atcctgtcat aaaaaatttg aaggaaaaca aacttgttcg
    18901 ccctttttca ttttcagata atacaggaag ttatcattca gaagctatag atgttgagcg
    18961 aggtacgaat cgcgtgattg atgcttcagg ggaaatccag tctgactttt attgctatgg
    19021 aatacctgtt gaaggtgtgg attggctaac tgcagcagta gctagacctt atacagatcc
    19081 atggaatctg agacaggcag ataaaatagc acaacttata ttgactgaca aataaaaata
    19141 cgcatgatga cttatctgtt ttttagaaag tcttcatgcg tatttttaaa taattattaa
    19201 cgagaaacgc gtttacgcat tgctttttta cggttttctt cgatcattgc ttctttttcg
    19261 tcaattggat cgatcactgt ttttttcaca gaagcaacca aaccagcaac ggcagcggct
    19321 gttgccagac ttcctactaa tattccagat acaaattttt tcattgaatc cacctcattt
    19381 attatttgtt atatctatta tggaacaaaa gagaaaaaaa ggctagtttt acgtttgttt
    19441 gtaaaccttt gcaaaaaatc cttgaaaatg tttactaaat gttttattat aaatttacca
    19501 tatgaattta cgaaatttta ctttgtttgt tttcaccata aaagtaaact tcgtttttta
    19561 aattatatag aatcaacaaa cacaatttta ctaatcaaag gagtgttttt tatgacaatt
    19621 ttagtactag gcggagccgg ttatatcggt tcgcacgctg tagaccagct agttcaaaag
    19681 gggtatcaag tagctgtggt agataatttg ctcacaggtc acaaacaagc agtacatcca
    19741 gatgctcatt tttatgaagg agacatccgg gataaagaat ttctacgttc cgtatttgaa
    19801 aaggaaccga ttgaaggagt aatccatttt gctgcaagct ctttagtagg agaatctgtc
    19861 gaaaaaccat tgatgtattt caacaacaac gtatacggta tgcaaatctt acttgaagtc
    19921 atgcacgagt tcaacgtgaa taaaatcgta ttctcttcta ctgcagcaac ttatggagag
    19981 ccgaaagagt ctccaatcac agaagataca ccagctaatc ctaaaaatcc gtatggtgaa
    20041 agtaaattga tgatggaaaa aatgatgaag tggtgtgacc aagcatatgg catgcgctat
    20101 gtcgctttgc gctacttcaa tgtagccgga gcaaaagcag atgcatctat cggagaagat
    20161 catacaccag aaacgcatct tgtcccaatc attttacaag tagctcttgg tcaaagaaaa
    20221 gccttggctg tttatggaga cgattatgat acaccagatg gaacatgtat cagagattat
    20281 gttcaagtag aagatttgat tgctgcacat attttagctt tggaatactt gaaagaagga
    20341 aatgaaagca acttcttcaa tttaggcagc agcaaaggat actcagtcaa ggaaatgcta
    20401 gaagcagcta gagaagtgac aggaaaagaa attccagcag aaatagcacc aagaagagct
    20461 ggagatccta gccgattagt cgcttcaagt gaaaaagcga gagaggttct gggttggaaa
    20521 cctgaataca cagatatcaa agcaattatc aaaacagcat gggattggca tgtcagccat
    20581 ccaaatggtt atgaagaata ggggaatgag atatgtcaat cagtcaaacg atttgtgatt
    20641 ttgccacact tgcgatcgaa gctggcggat ggatggaaat ggaccgctta tatttacaga
    20701 atcgtctttt agggatggta ggagaagatg ctctagaaag cattgaagta agaccagtta
    20761 aggaagaatc tgtagactta ttggatcagc ttgtcgctaa ggcaagagaa aatggtgttc
    20821 tttcagaatt gccagcagaa caagagattt ttgaagcgca attaatggat tttctcacac
    20881 cgccaccttc agtcgtcaat gcattctttg cacagcacta ttcgaaaagc ccagaggaag
    20941 caactgagta tttctttaat ttaagtaaga aaaatgacta catcaaaaca agagccatcg
    21001 caaaaaacat caaatttccg gcagagtcgg agtatggtga actggaaatc acaatcaatt
    21061 tatctaagcc agaaaaagat ccaaaacaaa tcgcagcaga acgcgaggcg gtaaaagtag
    21121 actatcctac ctgcatgctt tgcatggaaa acgaaggata tagaggtcgc ttgaactatc
    21181 cagcccgcac caatcaccgg attattcgaa tgaatctaga cggtgaaagc tggggattcc
    21241 aatattctcc ttatgcttat tacaatgaac attcgatcat tttatcagaa caacaccgcc
    21301 cgatgaaaat cacaaaggaa acatttgcta gactgcttcg gattactgaa gtcttgcctc
    21361 attactttgt tggttcgaat gctgacttgc cgattgttgg aggatcgatt ctttctcatg
    21421 atcattatca ggcaggacgt catgaatttc ctatggcaaa agcaccgata gaaaaactta
    21481 tcaaactagc agattatccg caagtcatcg caggagtcgt gaaatggccg atgtctgtga
    21541 tccgtttgca atcaacagag aaaaaattgt tgattgaagc agctaactca gtattggaaa
    21601 agtggaaagc atattcggat gaaacagtat ctatcagagc tttttcagca gatggtacac
    21661 cacaccatac gattacgcca attgcacgaa gaagggacaa ttactttgag ttggaccttg
    21721 tccttcgaga caacaatgtt tcagaagaac atcctgatgg gatttttcat ccacataaag
    21781 atgtccaaca tatcaaaaag gagaacattg ggttgatcga agtaatgggg cttgctgttt
    21841 taccacctcg tttgaaacca gaactagtag aagtagagca tttcttatta ggagaagaaa
    21901 accagatggc tgaatatcat cgtccgtggg cagaacaatt aaaaaaagaa catcctgatt
    21961 tgacgaaaga tacggtcacc gatgtcgtac aaaaatctgt aggtcaggtt tttgcacgtg
    22021 tactggaaga cgctggagta ttcaagcgag atgaaaaagg acaggcagcg tttgaacgat
    22081 ttactgcaat tttataaacg taaaaaagga ggagagacaa atggcaacaa ttaaagacat
    22141 tgcgcagttg gcaggagttt ctcctgctac tgtttcaaga gttctcaact atgatcctga
    22201 tctttccgtt ggcttagcca caaagcaaaa aatttttgaa gcagcagaac gcttaaatta
    22261 tacgaaacat cgtaaaaatg cagcaaatac taagcaaacg cttcgcctga tccagtggta
    22321 cgacgatacg gaagagcttg aagatcttta ttatttatct attcgtctag gaatcgaaaa
    22381 aaaggcagaa gaagcaaacg ttactttatt gaaagaaacg tgggattcca ttagtgatct
    22441 tccgacagat ggaacgatcg cactaggaaa attcgacgaa gctcagttat tagaaatgaa
    22501 aggatcccaa aatgccttgc ttttggtaga ttctgacggc acttctcaag gaatcgactc
    22561 tctagttgtc gactttaagt caagtgttca aaaagtcatt gaacattttg tctcccataa
    22621 agcgcaacat atcgctatgc ttgctggaac agaatatacg aagaaaaacc atcagcggct
    22681 aaaagatccc agaattatgg catttcaaca agttttggca gaaaaaggct acgacgaagg
    22741 tccaattatc gaagcagatt tttctgtcga gtcaggatat caagcggtga agcagtattt
    22801 aaaaacgaat ccgcatgttc ctgatgcgct ttttgcagct aatgatgcat tagcgattgg
    22861 cgcactgaaa gcgattcaag aaaaaggttt agtggtacca gaagatattt ctgtgatggg
    22921 tttcaatgat gtcagtgtag cgaaatatgt aacgccctct ctttctactg taaaagttta
    22981 taccgaatgg atgggagagc tagcagtgga gacgattctt caattaagta attccaaagc
    23041 accggtttca agaaagatta ttgtggaaac agagttgatt attcgagaat caacgaaata
    23101 aaagatggaa aagctgtggg gaaatgaagt gataccgcag cttttttgtg tttcaggaag
    23161 gggcaaa
//
LOCUS       DAMYXM010000038        23055 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.039, whole genome shotgun sequence.
ACCESSION   DAMYXM010000038 DAMYXM010000000
VERSION     DAMYXM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.039"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            82..2523
                     /locus_tag="PNS21_001692"
     CDS             82..2523
                     /locus_tag="PNS21_001692"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDI5159403.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2853..3557)
                     /locus_tag="PNS21_001693"
     CDS             complement(2853..3557)
                     /locus_tag="PNS21_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159404.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYSLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3744..3989)
                     /locus_tag="PNS21_001694"
     CDS             complement(3744..3989)
                     /locus_tag="PNS21_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159405.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4006..4857)
                     /locus_tag="PNS21_001695"
     CDS             complement(4006..4857)
                     /locus_tag="PNS21_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDI5159406.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5023..6027
                     /locus_tag="PNS21_001696"
     CDS             5023..6027
                     /locus_tag="PNS21_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDI5159407.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6268..6624)
                     /locus_tag="PNS21_001697"
     CDS             complement(6268..6624)
                     /locus_tag="PNS21_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDI5159408.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            6992..8179
                     /gene="tuf"
                     /locus_tag="PNS21_001698"
     CDS             6992..8179
                     /gene="tuf"
                     /locus_tag="PNS21_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDI5159409.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8367..10958)
                     /gene="mprF"
                     /locus_tag="PNS21_001699"
     CDS             complement(8367..10958)
                     /gene="mprF"
                     /locus_tag="PNS21_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDI5159410.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(10984..11745)
                     /locus_tag="PNS21_001700"
     CDS             complement(10984..11745)
                     /locus_tag="PNS21_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI5159411.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12023..12544
                     /locus_tag="PNS21_001701"
     CDS             12023..12544
                     /locus_tag="PNS21_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HDI5159412.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12603..13463
                     /locus_tag="PNS21_001702"
     CDS             12603..13463
                     /locus_tag="PNS21_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDI5159413.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13648..15963
                     /locus_tag="PNS21_001703"
     CDS             13648..15963
                     /locus_tag="PNS21_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDI5159414.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16145..17986
                     /locus_tag="PNS21_001704"
     CDS             16145..17986
                     /locus_tag="PNS21_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDI5159415.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18010..19119
                     /gene="rpoD"
                     /locus_tag="PNS21_001705"
     CDS             18010..19119
                     /gene="rpoD"
                     /locus_tag="PNS21_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDI5159416.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            19339..21438
                     /locus_tag="PNS21_001706"
     CDS             19339..21438
                     /locus_tag="PNS21_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDI5159417.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            21634..22497
                     /locus_tag="PNS21_001707"
     CDS             21634..22497
                     /locus_tag="PNS21_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI5159418.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            22602..>23055
                     /locus_tag="PNS21_001708"
     CDS             22602..>23055
                     /locus_tag="PNS21_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDI5159419.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         7762 a         4037 c         4807 g         6449 t
ORIGIN      
        1 aatcttagaa aaaaataact ggaacaagag ctatttgttt ttacagcaaa gaatggtata
       61 tttttgtagg aggtggctta cttgaaagaa gtcgttatga tagatgctgc aagaacgccg
      121 ataggaaagt atcgcggaag tttaagtcca tttactgcag ttgaattagg cacacttgtg
      181 actaaaggat tattagataa aacaaaactg aaaaaagata aaatcgatca agtaatattt
      241 ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca gacagattgc tttgaacagc
      301 ggtctccctg tagatgtccc agcaatgacg atcaatgaag tttgcggatc tggtatgaaa
      361 gcagtgatct tagcacgcca gctgatccaa ttaggtgaag cagaacttgt gatagccggc
      421 ggaacagaga gtatgtctca agcacctatg ctgaaacagt atcagtcaga aacaaatgaa
      481 tatggtgaac caatttccag tatggtcaac gacggattga ctgacgcatt ttcaaatgca
      541 catatgggat taaccgcaga gaatgttgca acacaatttt ctgtgagcag agaagagcag
      601 gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg ctgtcgaagc cggtgtattt
      661 tctgaggaga tcatcccagt caagatttct gatgaagacg tgttatctga ggatgaagca
      721 gttcgtggaa atagtacatt ggaaaaactg ggcacgttac gtacagtatt ctcagaagaa
      781 ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg gtgcctctgt ggtgatcctt
      841 gcatccaaag aatacgcaga aaataataat ctgccttatt tagcaaccat caaagaagta
      901 gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc caatcaaagc cattcaaaaa
      961 ttgacagata gatcaggaat gaatttatca acaatcgatc tttttgagat caatgaagca
     1021 tttgcagctt cttctatcgt agttagccaa gaactgcagt tagatgaaga aaaggtcaat
     1081 atatatggag gagcaatcgc attaggccat ccaattggtg caagcggtgc acgtatcttg
     1141 actacattag cttacggatt gcttcgtgaa cagaagagat acggtattgc ttcattatgt
     1201 attggtggag gtttaggctt ggcagtcttg ttagaagcga atatggagca aacacataag
     1261 gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg aacggcgcag tcaattaata
     1321 gaaaaaaatg tattgacaca agaaactgcg ttgatttttc aggaacaaac actttctgaa
     1381 gagctctccg atcacatgat cgaaaaccaa gtgagtgaag tggaaatccc aatggggatc
     1441 gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc cgatggcaac agaggaacca
     1501 tctgtcattg cagctgcaag taatggtgca aaaatctgtg gaaatatctg cgcagaaaca
     1561 cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa aatcggagta tcaagcagtg
     1621 atcaatgcag ttaatcatcg aaaagaagag ctgattcttt gtgcaaatga aagttatcct
     1681 tcaatcgtga aacgcggggg cggtgttcaa gatatctcta ctcgggaatt catggggagt
     1741 ttccatgcat acttatctat tgatttttta gtggatgtaa aagatgcaat gggcgctaac
     1801 atgatcaact cgattcttga gagtgtggca aacaagttac gggagtggtt ccctgaagaa
     1861 gagattctat tcagtatttt aagtaatttt gctacagaat cattggcttc tgcctgttgt
     1921 gaaatcccat tcgaacgctt agggagaaac aaagaaatcg gtgaacaaat tgccaaaaaa
     1981 atccaacagg caggggaata tgcgaagctt gacccttacc gtgcagcaac gcacaacaaa
     2041 gggatcatga atggtataga agctgtagtg gcagctacag gaaatgatac acgtgcggtt
     2101 agcgcttcga tccacgccta cgcggcaaga aatggattat accaaggatt aacagactgg
     2161 cagatcaaag gggataaact agttggaaaa ctaacggtgc ctttggctgt tgccacagta
     2221 ggcggagctt ccaatatcct gccaaaagca aaagcatctt tagctatgtt agatattgat
     2281 tcagccaagg aactagcaca agtaattgct gcagtagggt tagctcaaaa cctggctgct
     2341 ttgcgtgcat tagttacaga aggaatccaa aaaggccaca tgggacttca agctcgttca
     2401 ttagcgatct caattggggc tattggtgag gaaatcgagc aagttgcgaa aaaattacgt
     2461 gaagcagaaa agatgaacca gcagacagct atacaaatat tggaaaaaat ccgggaaaaa
     2521 taaaaaagca atatctttag aagaataaag caagatttac agttatttta tttactgcag
     2581 atcttgcttt tttctctatc agaaaatatg tttattatta gaatttttta tagttaaggt
     2641 taggcatctt aagaaatcag gcaactccaa gatatcttca ggtagaagag aagtcgccta
     2701 acattgtagg atcataggtg atttgttgga caggtcaagc tgatatttga gcctgatgtt
     2761 agaaggaatt tctttttact tgctgtagaa attattatat gactaaaaag gctgggattt
     2821 taaatcccag ccttttatga caaatagaat ttttatcgat cagtaatcgc tgtcgctgca
     2881 aattgttcga tcaaaggaat caaagtcaat ttgactttgt atttccgttc gagtgctttt
     2941 tcgataacga actcattttc agtagtcata gaaatagttg ctaatttacg aggttcatca
     3001 gcattttttt cattaataac tacttcatgt tcattttgtt ctgtgtaaaa tttaggatac
     3061 tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg cattcgtatc agttgagcgt
     3121 gtattttcag ttgtgtccgc ttttggtttt tccgtttttt cttctggcac actgactgtt
     3181 tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt catcgttttt caatcgttga
     3241 tcattccagt aagacacgat ttcaataaga agattgtcac ggaatcgttc gaaaatcccg
     3301 ttttctcctg aacgaattct ctttaataca gtattgaagt tgtcatcgat tggcacgttg
     3361 atccgtgtat cttcatttgt tttcccagct aatccgatcg ttaagatttg acggtttttg
     3421 ggatcaagca tgacttgtaa cggcaaacta taacgtcgca agctctcttc ttctagagaa
     3481 cggaataatt gtcctcttac accatccaat gaaggacgga attgatttaa taatttttgt
     3541 ttctgttttt tcttcatatg ttctctcctt cgctcatacc ctttcgatta taagaaaaaa
     3601 acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta tacgcaaatt acaacagaaa
     3661 aatttgcgta ttttcccgtt ttttgaaaga aaaataaaaa atggaacgga ttatgtatat
     3721 atacataatc cgttccattc tttctatttt ttcggtatca aggcaaaaac aggtagttca
     3781 ggtgaataaa tgacttcatg ttcgtgccag ggtttgacca ttagcgaatg atcgaaccgt
     3841 tttttcagtt cttcgatgat ttgatcatgt gtccagtttc ttgcaggaaa atgttggatt
     3901 ttctctgcat cgatcaaaaa gacaaaatcg gaatgaaaag tctgggtaac ttcttccgag
     3961 agagaattca agcgttcgat tggatccata aaatcacctt tcgacttaaa attgggtagt
     4021 atctgggtct tttgctttgc cgactacacc atctaactga tcgatttgtt tcatttcttc
     4081 ttctgttaat tcaaaatcaa ataattcagt attttctttg attctgcttg gtgtcactga
     4141 cttaggaaga ggaaggaatt cgtgttgcag cgaccaacgc aaagcaactt gtgcaatcgt
     4201 cttgtcatgt gcttcagcga tttgtttcat ttcaggcaca tcgaagattt taccagtgcc
     4261 taatggacta tatgcttcga ggatcatatc atgttttttt gcatactcaa ctacagttgt
     4321 ctgtaattcg cctggtgcaa ggaaaacttg attgaccatc ggcataattt ttgctgtttt
     4381 tgctagtgta tctagatggt ggggtaagaa attgctcaca ccgattgctt tgattttacc
     4441 tgcttcatac agttcttcaa atgctcgcca tgtatctgca tttgcttgtt cccaattatc
     4501 acgaaaagca acagggtttg gccagtggat caaaaacaga tctaaataat ctgtttggag
     4561 cttcttcaat gattcttcaa aagaagacat cacaagttca tagctgtggt ttgcattcca
     4621 taattttgtt gtcaagaata tctcttctct aggaattcca ctttccttaa tcgcttggcc
     4681 gacgctttct tcattcttgt atccttgggc agtatcgata tggcgataac ctgcagccag
     4741 cgcttcttta acagaggaaa cagcgacatc cccatcaggt gtttgccatg tgccgaatcc
     4801 tactaccggg atttcgtaac catttgatag acgataagtg cttgttaatg aattcatact
     4861 ttcagctcct tttttattcg ttcaactata agtgtagcgc atttttcgaa aaaaagaatc
     4921 cattttgttt ctactcttga aatttttgtt taaaagaaaa tgattttgat tctggtattt
     4981 ttgatttttt ttccgtaaac taagacagaa gggaatgaga aaatggatat ctatttaaaa
     5041 aaagcaattc tgcatattat tgacagagaa actggttctc cagtcttttc acaaaaagag
     5101 ctggatctga caaaagaata tattcgtgat tttcttcaaa agaagatcca aaaaatctct
     5161 tctgctcaaa caaaaactgg acaactagca gaagaaagca tgatcgttga atcacttaaa
     5221 atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc
     5281 taccaagaaa gtgaagaagc acctagtgca gatgtatttg ttgtcttgta tgaattagat
     5341 acagtgatgt acttatcttt attgaaagtc aattatcgtg aggcttacac tcactttgtt
     5401 gaagcagacg aagcagggat cgacaaccgc ttgattttga atcgagcaat tctaggggcg
     5461 aaaacacaaa aagcagatga agcattcgct gtgaatttga ctgattggac ctacgaatta
     5521 attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt atttttctgg aagagtgatc
     5581 gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag tgatccaaaa agtagccaag
     5641 catcttggaa aaaaatttga gacagaagaa tttgatatca tggctgattt gaaagaagct
     5701 gtctatgata cgatagaaga aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc
     5761 ttcaaggaaa acatcacggc aaaaatggct tttaaagaag aagtacaaga gcaaggattt
     5821 gtcccagaag cacctcctgt aaaagaagta caagaaattt ctgagaaaaa atttggaaaa
     5881 caaaaattca agctatcaaa cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac
     5941 ccagatttga ttgaatttgt gaataatccg gatggtacga tatccgtgat gatcaaaaat
     6001 gtggatgaag tattgaatcg tttataaaaa tgactagaat attaggtgtt caagtaccag
     6061 tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa agacgaatat aacaaaaaaa
     6121 gagcgtgaga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg
     6181 gaagcagaag caactccttc ttatttcttg gagttaaacg cttctgtccc agcctctttt
     6241 tatctaataa aattttaaga ccatttacta attatcgatt ttttctgccg catttttagg
     6301 cacttcagaa gcctttattt cttcccagct taatacacct ttacgaggat tgactttgag
     6361 tttaagatac gcattcatct ttaatggatg ttctctttct tcgcgaagcg tcacctgttt
     6421 tttttcacca tctttattat aagccgcttg cttgtaggta taagtcgtga atttctctcc
     6481 gttatctgct tgggtagctg acatttcgcc attcgtcgtg atttttgtat aataagcttc
     6541 gccgccgtaa aaataatgat aagctgcaaa acttccgccg agaaatatgc ctaaaacgac
     6601 aagtacgcca attaattttt tcatgtactt cacctctttc cttattatta acgaaataga
     6661 agatttcgtc tattgaaagt tgttggcaac agctgaattt taaacttttc ctcattttgt
     6721 tcttattgct tcaaaagaaa gttaaggact atcttgaagt ttttctcaaa aaacacattt
     6781 ttttatttat aaaaggtact tttttcgcgt ataagacaga cgagtgctct tagttgactt
     6841 tgaccaatat tgacgtataa taagcttagt tcgaatctta caaaaagtaa gtcggacgta
     6901 ttctttttga tttagtcaaa aaaagactat aataaaaagg tgagagctga aatttttcag
     6961 tgatctatat tatttaggag gtttgtaaat tatggcaaaa gaacattatg acagaagtaa
     7021 accacacgtt aacattggta cgattggaca cgttgaccat gggaaaacta ctttaactgc
     7081 tgcgatcact acagtattag gcaaaaaagg cctagcaaat cctcaagact atgctagtat
     7141 tgatgcagct ccagaagaac gtgaacgtgg gattacgatc aataccgcac acgtagaata
     7201 tgaaacagaa aaacgccact acgcgcatat tgatgcacca ggacacgcgg actatgtgaa
     7261 aaacatgatt acaggggcag cacaaatgga tggtgcaatc ttagttgttt ctgcaactga
     7321 cggtccgatg cctcaaacac gtgaacacat tttattgtca cgccaagttg gtgtaaaata
     7381 cctgattgtt ttcttgaaca aagttgattt agtcgatgat gaagaattga tcgatttggt
     7441 agaaatggaa gttcgcgagt tattgagcga atatggtttc ccaggcgatg acactcctgt
     7501 gatcaaaggt tccgcattaa aagcattgca aggcgatcca gatgctgaag ctgctattat
     7561 ggaattgatg gatacagtag atgaatatat cccaacacca gaacgtgata cagataaacc
     7621 attactattg ccagtggaag acgtcttctc aattacaggt cgaggaactg ttgcctcagg
     7681 tcgtattgat cgtggtgctg ttcgtgtcgg tgatgaggta gagatcgtag ggatcaaacc
     7741 tgaaacacaa aaagcagttg taacaggtgt agaaatgttc cgtaaaacgt tagattacgg
     7801 ggaagctggg gataacgtag gcgtgttgtt acgggggatc caacgtgacg atatcgaacg
     7861 tggacaagta cttgctaaac caggttccat tactccacat acaaaattca aagcagaagt
     7921 gtacgtgttg acaaaagaag aaggtggacg tcatactcca ttcttcaaca actatcgtcc
     7981 acagttctac ttccgcacaa ctgatgttac aggaacaatc acattgccag aagatacaga
     8041 aatggtcatg cctggtgaca acgtgacgat tgatgttgac ttgatccatc caatcgctgt
     8101 tgaaaacgga acgacattct caattcgtga aggcggacgg acagtaggtt caggtatcgt
     8161 tacagagatc gaagcataag aaacgcgact cgaatatttt ggtaatgaaa aatgtttaaa
     8221 ataaaagagc ggtccaaaca ggatttctgt ttggaccgct ctttgttctt caattgtata
     8281 agaagagatt gtagaagcat gaactttcgt tttacttcag tctcttttat tcatcatgag
     8341 agtgttcatc tatcggtatg cagtttttaa tactttcttc gtatccattt tttcaaacca
     8401 cttattctac cttgtgtctg ctcaactggc gcattatcaa taatcagcaa agcaatcata
     8461 acatatgcaa tccaactatc tctagaatat agagtataac gtgagaccca gtttgtagcg
     8521 tacttgcttt tatattctct taatccatgg aaagaataaa aatgagaacc aaactcgtaa
     8581 accaagtatg caatcctttc ctgtaaaaaa ctctttctgg aagttccgac atttgaaaga
     8641 ggggccatcc ctaaattgaa ccattggata tgttcttctt tcatataggc aaacaaatgc
     8701 aagaacaaga aatccatact gccagaaggt gcttttttag aatcatatcg catcagatca
     8761 atcgttccta ctttattgtt cgtgtaagta ggcatgatcg ttgcaaaagc gacaatttct
     8821 tcttgggtat ttttgacaac tgcaataggc gctctttgta gataatcttc agagaaaaag
     8881 cctaaagaaa accctttttc ctttcgtgaa cccaaccaat tatcagagat atttttgaaa
     8941 gtactcattt gctcagtaga aaatggtggt tgtaagacat caaatgtaaa tccttctcgt
     9001 tcgatccgat tcaagacagc acgagtgccc ttcatttttt tccctgaagt tgtaaaacta
     9061 ttcaggtcta cataagcttc ttcccccatc ttaatgaaat cgtagccgaa ttcatgcaaa
     9121 atcataacaa tctcttcgct tgtttcgtag aaaactggta aatagcatag tcgatctgtt
     9181 tcttcgataa aagcttcgat tgcttctgga aaatcttctt tcttaccaga aggatcaccc
     9241 attacaatac atttattatt gaagcaagcg aattggagaa gaacagttgg ttcaccgtct
     9301 ttttcatagg caaacatccg cttatctttt aagaagatca attgactatc ggagtttcct
     9361 ccataagttg taaggatctt cagggccttt tcttcattaa aggcttcgcc aatctgtttc
     9421 ttttctcctt gaaggaaatg aacaaataga acgatcatga aactaacagc gatgatggcc
     9481 aacaatcccg aaaaccaaat cttttcagaa ggaaataaga aaaaggaaat aaaatggtga
     9541 cgccgatgag ggaaatccgg cagattgtat accccaataa cgatgtaaag caaggacagt
     9601 gcaccaataa tgattccatc gatcgtacgc cactcccatg aatagatcaa ttgttcccga
     9661 aataattcgt ttttagaagc aagaatgatc agaagcagaa tagacaagaa aaccaccggt
     9721 gtgaaactaa aatcacttaa taaaacataa agtaaagcta gaacaatcaa gaagattgta
     9781 ggcaaatatg ctcgtttcac acgtgctgcg atccctctgc ccataatgat taaaagaaat
     9841 cccaatagta tactaggaaa ttggataata aaatgaaatt tcaatgggtt caaactatga
     9901 agccagcgaa actctgtaaa agcttgcggg atagtggcag aaagtactag catgattcca
     9961 gaaaagtata ataagacaac cacgatttta tgcgcaattt ctgttgctaa ttgtttaggg
    10021 ataccagaat aacgctgatc aaaagtggaa ccgatatttt tgaagaaaaa gacgattcca
    10081 atcaaaaacg gaatgatgta gtagaacagg cgatagagca agatccacac aacgactatt
    10141 tctcttggca caccaatagc ggacaacccg ataatcatca ttacatcgaa actcccaatt
    10201 tctcctggaa tcatagacac gattcctatc actgaagcag caatgaataa tggaattgtt
    10261 tgccacagat caatccggat ttccattaaa taaccaacag aaaggaaact aaacaggaca
    10321 ccggtccatt ccaacaatga aactagcaat agctgtcctt gagttttaag tgaggtacca
    10381 ccaaggaatc ctttcttttt gaaagtagta aataaaaaga cgacaggaaa atatagaccg
    10441 ccgccgatca accatatcca atattgcttc aaaaaaggag caactggcgt gaagactaca
    10501 agtaaaaaag aaagcagact gtaaatagaa agtccagcca tcaaaaacaa gaaaactttc
    10561 gataatgctt ggatcacgtt tctgctctct ttcttttgtc catatagttc agagcgaagc
    10621 cccacactaa caaacccgcc gaaaccggca atattgttga tcgtattgat cagccagctt
    10681 gtctcaaaca ggtaacgagg attctgcttt tgttcaagca tccgattcaa aataatatca
    10741 taaccaacca taggtaacac agagataaga ccaatcaata gcattagtcc gcttttccat
    10801 acaggtatag tggcaaacgt ttctcctaat tgctggatag atagagtttt accgatggac
    10861 atcaattcac cgacaataat gacaatgaca gaaatcaaaa agattgtttt gaacagtcct
    10921 aggtggtttt tcagccattg tatggtattt tttaacaaat aaatcacctt ctattcctaa
    10981 tagttatgtt tcagaaagaa attgaagaac ttctcgattg aagcgttcag gatcttttct
    11041 agctaattca tgtccttgtt ctttcacaag cacaaaggaa gcttttggta tagttttagc
    11101 tatatacaaa gaatgtttta gcttgattac atcttttttt cccacgatga tcaaagtggg
    11161 ggagttgatt ttttttaaat cattttctgt aagcccgatg tcatgcagca aaagtttgat
    11221 aactaaaaga tttttgcgaa gacttgggcg aaataaagag agtaaccata cccatgcata
    11281 atgaatatta ctgatgactc tggcagaaaa acgaacgcct ttaacaagcg tgtttccaga
    11341 atttaaaatc aatcgatgaa cttttttagg gaagctgctg gcaaatacca gtgctagatt
    11401 tgctccatca ctaaatccaa gaaaatctgc ctgatcgatt ttttccagca acatgatcgt
    11461 attcagatcc tcggccatta actgaaaatt caacatagat gcttcgtttg tcgaacgacc
    11521 atgtcctcgg ctatctacta gatacacggt atagtagcgt tcgagtacag ggacctgttc
    11581 tgaaaaaaag cggccgctgc cgtcattgcc atgcagtaaa aagagaggga agccttgacc
    11641 gctcttttca tagtaaattt cgctcccatc tggcattttt gcatatttct tttctttttt
    11701 cacataatca cctactgtgt aaagtataac gattatatca gtcattgggc aattgttttt
    11761 actgaaaaac aacgatcatt ttcgtaagat gtcaaaaaat acaggaaaat tcattttttc
    11821 ttaaggcgta cacagaaatc aaggaagaaa tagccaagaa gataaagaaa tagtaagata
    11881 aaaaaagatg tcaaaataac taggtgatga aatgaagcga aaacgattat ctattatccg
    11941 tattcttttt tacatgatca ttctgacggt tattggtgcg gtgttgtttt tatgaggaag
    12001 aacttatcgt tcggtaaaac gagtggaaag ctatcaaata gaaataacaa cagctgcaaa
    12061 aaattatcat atggaaaact acgagaattt gatcaaggcg ataatattga ccgaatcaaa
    12121 aggaaaaggg acagatctca tgcagagctc ggaaagtctg catggagaag ctgagatcat
    12181 tgataatcca gctgacagta ttgatcaagg tgtcaaatat ttggcggaaa tgatagtaga
    12241 agcaaagaaa caaggatgcg atttatggac agctgtccaa gcatataatt ttgggaaaga
    12301 ttatattgcc tatgtaaaaa atcatggcgg agaaaacaca gtagaattag cagaagcgta
    12361 ttctaaagat gtgctgagcc cacttttagg aaatgaggaa gggaaacaat accgatattg
    12421 gcggatccag tccttgcttt acaatggcgg ctatctctat cataatggag gtaacatgtt
    12481 ttatcctgat attgtcaaaa tgaatgaaaa aataatagag acctatgaaa aagtctttaa
    12541 gtaaaatagc aggaaatgtt caatcatgaa taaaattaaa tgaaagaagg agtgcgcaaa
    12601 tagtggaaaa aaggcaaaaa atggaaaatc aagaagtggg gtacacaacc aaagactgga
    12661 tgttacggtt tgtgaaggga atgtttatcg gttcaggatt tatcttgcca ggagtcagcg
    12721 gtggtgcatt agcagctgtc tttggcatgt atgagcggct gatttctttt ttagcccacc
    12781 ttacaaaaaa tttcaaagaa aatgttttgt tttttctgcc tgttggtcta ggcggagtga
    12841 caggcatctt tctactttct tttttagtca gttttttatt aggtgcgtat gaaacaacga
    12901 tattatggtt ttttgtcgga tgtatcgctg gaactgttcc tgcgttatgg aaagaagcag
    12961 ggaaaaaagg aagagatact tctgatgtga tcatcatggt tgttactttt gttctggctt
    13021 atttgttttt attatacggc gctcaattat ttgacgggca agtcgatcag aattttggaa
    13081 catggctgat tgccggcgga ttgattggtt tgggtatgat cgtcccagga ttgagccctt
    13141 ctaatttttt ggtgtatatg ggattgtata aagcgatggc tgacggattt aaagaagtac
    13201 gacttgaagt gattgtgccg atcggaattg gtgggattct ttgtgttctt ggattgtcaa
    13261 aaatcatgga ttacattttc tcaaaggcat atagcaagct attccatttc attttaggtg
    13321 tagtttttgc ttcaacgatt atgatcattc ctaccgatta cgcgggattg ggagttgctg
    13381 gggtactagc ttgtcctgtt ctttttatag ccggtacagc actaggttgg tggatgagcc
    13441 gtttggagga acaatataag taaagataga ttgataataa aaacagcaca cgggtgattc
    13501 aaacaaaaag gttggcatct tttttgtaaa atcattcgtg tgtttttcta taaattttat
    13561 aaaaacaagt tgtttttaat cttggaacac gctagactta aacacgtgat tatttttgaa
    13621 agttggcgga aaatggagaa aaagaaaatg aatggactta cacagcaaga ggtagttcag
    13681 aaaatggctt ctggccagca aaatgactac caggaagatg catcaaaaag tacaaaacaa
    13741 atattcagcg ataatatatt gacgttattc aattttctga actttggtat tgggatttgt
    13801 ctgttatttg taggtgcgta ttccaacatg gcgtatttag caattattgt cgtcaatatc
    13861 gttattggaa tttatcaaga aatacatgca agaaatcttg tacggaaatt atcgattgtt
    13921 tcccaatcag aagtactagt gatccgggac ggtaaagaac aatcgatttc tactaaagag
    13981 ctggtattag gagatttggt aaaaatcgga gcaggagaac agataccagc cgatttacag
    14041 gttatatctg gacgggcaga agcaaatgaa gctcttttga caggcgaatc tgatttgatt
    14101 gtcaaagaaa ctggagctga attgctttct ggaagctttt tgacaagcgg ttctgtcatg
    14161 gctgaagtta tccgagtagg tgcagataat tatgctgtcc gcttgactag cgaggcaaag
    14221 actcacaaag ctattcgttc tgaacttgta gattcgattc gcaaagtatc caaattcact
    14281 agtttcgtga ttattccttt ggggatcata ctatttttgg aagcactgtt catccgcgat
    14341 gcttctatca aaatttctgt tattgcatct gcagcagctt tgttaggcat gcttccaaag
    14401 ggtttggtat tgctaataag tattgcatta acgacagggg taaccaaact ggcaaaaaaa
    14461 cgaatccttg ttcaagacat gtattcaatt gaaacgctgg cccatgtcga tacattatgc
    14521 ttggataaaa cagggaccat tactgaaggg aaaatgagta tccagcgggt agaagcacta
    14581 catccgctgt acgagaaagc catcccagaa attatgggga cgtatctggc agaaagcagt
    14641 gataacaaca tcactatgcg ggctatgaga gattattttg agctttctaa tcggtatgat
    14701 gtgacaaaca aagtcgcatt ttcttctgat cgcaaatggg gcgcgatgga atttccagaa
    14761 ctgggagtcg tgtacgtagg agcgcctgaa cgtttggtgg aagaagatcg gcttccggac
    14821 agtctaaaaa cagcccaagc gaatggctat cgcgtactta tgttggggat cgctccaaat
    14881 cagcaattga acgaagaaaa accagcattt gttgagccgg ttgctctgtt tgaaatcgat
    14941 gatccaatcc gaaaaaacgc tcaagaaaca ctagcttatc ttcatgagga aggtgtggat
    15001 ctgaaagtga tttctggaga caatcctttt actgtctcca atatcgctag acgtgcagga
    15061 ttaccgaatt atgatgcgta tgtcgatctt tctcaaatta ctgaagaaat ggaagtgcga
    15121 gaagctgctc atcgctatac ggtttttgga cgagtgagtc cgcagcaaaa gaaattgctg
    15181 gtaaatgaat tgaaagaaag cggcagaacg gttgcaatga caggcgacgg tgtgaatgat
    15241 gttttggcgt taagagaagc agattgcagc attgctatgg cagaaggtga tggagcaaca
    15301 cgtcagattg cgaatctggt attgttggac tctgatttta ctacattgcc agaagtcctg
    15361 tttgaaggac gccgagtggt caacaatgtg acgaaagtct caggcatctt ttttatcaaa
    15421 acgatttatt catttatttt atcgattata tgtgccgtta cagcaattgg atttccgttt
    15481 atccctatcc aaatcacgct gattgattta gcgattgaag ggtatccttc cttcttttta
    15541 tcttttgaac aagataaaag aaaaatcact taccgttatt tgcctacagc acttattaat
    15601 gctttaccaa atgccttgct tgtagtagtg aatatcattg cggtgtatct acttggaaaa
    15661 acattcgcat ggggaaatgc tgaaactact actttgatgt attacttgct gattgggatc
    15721 agttgcatgg cagttgtaaa agcttgttat cccttcaacc cattgcgcat cttcctagca
    15781 gtaacgacga tcgtagggat atatgtagct gcaatgctgt tccatggaat tttggaagta
    15841 aacttagggt tgggagagag cgtaggatac ttctttgtat tcatgctcgt aaatattgtt
    15901 cttcgagtat tgtactggaa ggctgatatt caaaatcagt tactgaaaag agtagcaaat
    15961 tgatgcttaa tgagatagaa atgtgttata cttgaaaaag caatgaaata tgatacaatc
    16021 aaacatgtat aattgtgtcc tttaaaatcg cactgatttc agttagtgcg attttttacc
    16081 ggttattctc ctgaaatatc ttctgttaaa cccaaaagat atcaatagag cagggggtga
    16141 aaaaatggct caacggattc ctcaagaggt tatcgaaaca gtacgcagtc aaacgaatat
    16201 cgttgaggtg attggacaat atgtccagtt gaaaaagtct ggtaaaaatt acttggggct
    16261 gtgtcctttt catgaagaac gtacaccgtc tttttctgtt gcagaagaca agcagatttt
    16321 tcattgcttt ggttgtggta aaggcggaaa cgtgtttact tttcttcaag aactagaagg
    16381 tctaagtttt ccagaagcag tggtaaaagt ggcagacttc gaacagatac cgttagaaga
    16441 tcaatggcgt cagcgtacta tagctgtcca aaatcctgaa tctgctacgg ggaaactgat
    16501 tgctgctcat gaaaaagcag cggaaatcta tcatcacatg ctattgcata caaaagctgg
    16561 acagccagct ttagaatatt tgcaaagtcg tgggttgtct ttagaattaa ttgaagaatt
    16621 caacataggt tttgcaccta atgagcgttc ttttcttcag caagtattca agaatgaatc
    16681 aatttcaaat gaattgttag aacgctctgg attgtttgtc acgcatgatg atggccgtat
    16741 gtctgatcgt ttttatcaaa gagtgatgtt tccacttcgt aattctcaag gccagacgat
    16801 cggtttttca ggacgctggc ttgcacctga aaaagggcag gaaaaagatc agcccaaata
    16861 cttgaatagt ccagaaacag aactgttcaa taaaagacaa gttttattta atcttgataa
    16921 agcaagaagc gatatccgga aaaacggaga aattttgttg ttcgaagggt ttatggatgt
    16981 gattgctgca tggcaggctg gtatcaaaac tggattagct tctatgggga caagtctaac
    17041 agctcaacag atccaacaaa tggaaaagct gacgaaagaa ttggtctttt gctatgacgg
    17101 agacaacgca ggttttgaag caaccaatcg agggattgaa ttgcttcggc aaaacagtcg
    17161 attagcactt tctgtcgtag tcgttccaga aaagcttgat cctgatgaat atctccgtaa
    17221 gtatggagaa gcatctttcc aagaattagt ggctcatggg cgcgaaacag tctttacttt
    17281 taaatcacgt tatttaaaac aagggaaaaa tttagataat gaaaaagaca agatcgaata
    17341 cctcgaagaa ttactagttg aacttagccg agttgtttca ccaatcgaac aagatatgta
    17401 tctatctcaa ctgtcaacag agtttcagat cggcagagag acgatacaaa aacaactgcg
    17461 cgatcttcga aggacaaatc agcaaaatcg tcaagaacct caggaaagtc aacacactca
    17521 ggttacgaga caagaaactg cacggaaaaa gagacctttg acgcaagtag aaaaggcaga
    17581 gcagattcta ttatatcgtg catttaaaga agtttctgtc cgtaatattt taaaagaacg
    17641 agaatttcag tttattcatg atgcatatgc agaagtttat tttttattcg atgcatatgt
    17701 ctcacaacat tatgaattca accttgcaga gtttttagac tttttgcaag atgaaaattt
    17761 aaggcggttg gtggtggaaa tcgaatattt accggtggca gaagaaagtt cgccacgaga
    17821 aatccaagac ttgctagatg tgatcagcaa atctggctta gctgatgaaa taacattaaa
    17881 aaaacaaatg cagcaagaag ccagacgaac tggaaacaaa cagcttgagc ttgaattgac
    17941 gattgaaatc atcaatttaa cgaaacagtt aaaacaagcc aaatagctac tcaagggggg
    18001 ccttctttta tggcaaatgg aacagataaa aaaacgtatg aagcagcagt agcagctttc
    18061 atcaaagaaa acaaacccaa acgacaagtt gtatatgatg atttatccaa tcagcttgct
    18121 acaccattca ctttgaatgc agatgaaatg gacaaattga tccaaaagat tgaagacgca
    18181 ggtatcagtg tggtggatga aaatggtgat ccatcaattc acagcttgaa gagtgctgag
    18241 aaaaaagctg aacaagcaaa aacagaagat ctttctgcgc caaccggtgt aaaaatcaat
    18301 gacccggttc gtatgtatct gaaagaaatt ggccgtgtct cccttttaac tgcggaagaa
    18361 gaagtagcgt tagcattgaa aatcgaagaa ggcgaccaag aagcgaaaca gcgtctggca
    18421 gaagcaaacc ttcgtttagt agtaagtatc gctaaacgtt atgtaggacg aggcatgcag
    18481 ttccttgact tgatccaaga aggaaacatg ggactgatga aggcagttga aaaatttgac
    18541 taccgcaaag gatttaagtt ttcaacctat gctacatggt ggattcgtca agccatcact
    18601 cgagcaatcg ctgaccaagc acgtacgatc cgtattccag ttcatatggt tgaaacaatt
    18661 aataaattga tacgtatcca acgtcaattg ctgcaagacc ttggaagaga acctacgcca
    18721 gaagaaatcg gtgcggaaat ggatcttcca acagaaaaag tccgagaaat cttgaaaatc
    18781 gcgcaagaac ctgtttccct tgaaacgcca atcggtgaag aagatgactc tcacttaggt
    18841 gatttcatag aagaccagga agcaacaagt ccagcagaac acgcagctta tgagttacta
    18901 aaagaacaat tggaagatgt actagataca ttgactgatc gggaagagaa tgtccttcgc
    18961 ttacgttttg gtttagatga tggccggacg agaacattgg aagaagtagg gaaagtattt
    19021 ggtgttactc gggaacgtat tcgtcagatc gaggctaaag cattaagaaa attgcgccat
    19081 ccttctcgtt caaaacaatt aaaagatttc ttagaataag cgtggactat tgaccagaag
    19141 agaaatcagt aaaactgttt ggtttatttt aaaagaaaaa aagcttgaga ctacgcagtc
    19201 tcaagctttt ttttctatag gaaggaaatt cattcattat ttttaagtaa atcctaaaaa
    19261 taagctattg gcgtgacgta tagaatatga tagattaaat ggtagcgtaa acctagtaaa
    19321 ggagccgatc gctaagtgat gaaaaaatgt ccaaattgcg gatcgaaaaa aatggagcag
    19381 aatactatct gtccagactg cggtcatcaa ataactgcag aagatcagca aataaaagaa
    19441 tcaattgaag aaacaaatac agatgcagct atgcaggata aatcaactga agatgcagtc
    19501 tttcctgata cagaattaaa cgatccaatc gaatggtcgg aattgaaaga tttacctcta
    19561 gaatctgtaa tggaattatt cgaaaaagca gaaccagaag acgaagaacc acctgctcac
    19621 gatcaagtaa ctaatataga gaaaaatgaa cagaagaaag caactgttca tgagacttcg
    19681 gaagaatcag gaaagaatca agaagaaacg cttgaatcaa aacaaaaaag aagagtagct
    19741 gaactgaaag aaatcgtcga taatgaggaa gaaaattcga tactatccgc ttatatcaaa
    19801 gcacatcgtg aagatacaaa agaagagcac gcgaaagagc tgctcaaaat gatcagtgaa
    19861 aaaattgcac aggaaaacaa agtagagcca gaaaaggaac aagaaactga aaaagactct
    19921 gatagagaaa acgccacagg taaagaaaca gtaaaaataa aagacgtaaa aacggatatc
    19981 acagaaaaaa ctgaagtatt tactgaaaaa caattagaaa aggaaaaaac gaataaactg
    20041 ccggaagcag agaaaaaaga aatactcgaa aacacagaca aaaagtcaac agagcccgat
    20101 gaaataaata gtgaatccga tgctgaaaat caatctgtaa gcaaagaggg tactgatctt
    20161 gaagcaaaaa tagaaaaaga agagaaccag cctaagctgt ttgaagcaga agcgaaatca
    20221 ataacaatcg tgccagatac tgaaaaaaca catcaagaat caaacttaga aaaaacagat
    20281 ccagataaag cgcaaaagaa aaaatcaaaa aaagtacctt atctcgtttt agcagcagta
    20341 cttctactcg gtggaggaag ctgggcttat tatgaccatc atcaaaaagt agaagccgaa
    20401 gtagcagcag aagcaaaaag acagcaacaa atggaagaac ttcatgagaa acttgctgcc
    20461 ttttatacag atgatcacca tcaatttatt cgtgcagata tgattcatca agatttatca
    20521 aaactaaaaa atgatttatc cagtcttaag aatgaaaaag gatattcaga gttagaaaca
    20581 acttaccaag atatccaatc taagatccga gatatccaaa aagtgaatga gtggttcata
    20641 gcacctgcca tcgtcgatga tcatctagca gatgacatca agttgaaagc agataaagag
    20701 atccaagcaa tcgaaacgga taacacagat tttggaaaat tgattcaaca agcacaaaca
    20761 gaagctaaaa atcaatatgc tgaattgcaa acagctaaag aaaaaacagc tatagtcttt
    20821 aaagacggaa aagtagtgga ttcagcaaca cgcgaccaat acaagacggc aaaagaagca
    20881 gtagaaaaag tgaaaaatac agatctatct gctcagttga atgaacaatt gaaacaagta
    20941 gataagatgc tcactgataa agagaaaaaa gaagaagagg aaaagaaaaa agcagaagaa
    21001 gcgaaaaaag cagctgaaga acaagcaaaa caagccgctc aagcagcagc tgcagctcaa
    21061 gccgctcaac aagctgcggt cgcacaagca gcacagcaag aagcggcatc ctcgacccct
    21121 tcctcttcaa actcagcgaa tcagccaatc aaatctgcca gagcaagtga tattgccgat
    21181 gcatctaatc cagcatggac gtgggcacca ggtgtccaaa gcaaagtgat tgctacatgt
    21241 atccaacgtg gatatatcgt tgaaggaggt tacagattag aaaaagccag aatcgaaaat
    21301 ggggaaggct attataatct atatgccact tcaacaaaat ctgcattgat gaaaggcatt
    21361 ggagaaagtg ctttgccatt ttacatcgta accatcaact gtaaaactgg atggttcgga
    21421 ggaaatggga gcaattaatt ttaggcaaat gaaagaggct gtgagcctgc cgtttaacac
    21481 cgtttattcg aaaaagggga gtatgacaca actgttgtca tactcctttt ttcacctttc
    21541 caaaccaagg ctcagctatt caagtagata acattatgtt atgatagcaa agaaatgtat
    21601 taagaaaacc tttcactata gagggagaaa aaaatgaacg atttattagc aactatattt
    21661 acaggtatta ttattgatga aaatgaaaaa aactactttg tccaaaaaaa cggacaaacc
    21721 tttggcttaa gcaaatcaga aggtgaacac aaaatcggtg aagcagttga aggattcggt
    21781 taccaaaatc aaaaacatga aaatcggatc actacacaaa ttcctaaaag ccgtatcggt
    21841 cattacgctt ttggaatcgt tactgctaca cgaaaagatt taggtgcgtt tgtcgatatc
    21901 ggtcttccag ataaagactt tgtcgtatct cttgatgaat tgccgacaat gacagagcta
    21961 tggcctaaaa aaggggatcg cttgatgatt tcccttagag ttgatgcaaa agaccgtatt
    22021 tggggcagtt tagcagatga aaaaatattc aaatctttaa gcaaatatgg aacagaagaa
    22081 atgaaaaaca aagatgtgat aggaacagca taccgcttga aattaacagg tacgtacgtt
    22141 ttaacggaag atttctatct tgggtttatc catccttctg aacgatataa tgaacctcgt
    22201 ctaggcgagc agataaaagg tcgagtgatt ggtgtccgac cagacggtgt actgaatctt
    22261 tcattgaagc caagagcata tgaagctata tcagatgatg cacaaatgat tttaaccatt
    22321 ttagaacgtt ctgcagatca gcaaatcaac tttacagata aatcggatcc agaagaaatc
    22381 atgaaagctt ttggtatcag caaaggagca tttaagcgcg cattaggtaa tcttttgaaa
    22441 caaggattga ttacacaaga aaacggtgtg acaaaactag tcaaaaaatc taattgagaa
    22501 tcccttgcat gtttttctta actagcttat aataatttta attaacatag tgttatatct
    22561 ttttttattt gtaattatta taaattgttg gggttgagta aatggtcatg aatgctattt
    22621 cggcgatgaa aaaaacaaaa cagcagctac atgaagcggg gtttaagctg acaccgcaaa
    22681 gagaagctac tctgcttgtg cttttggaaa atgagaaaga gcatttatct gcagaagaaa
    22741 tcttcttttt agtgaaaaaa aagagtccgg aaattggact tgctactgtt tatcgaaccc
    22801 ttgaaatctt gacagaattg aaaattgtcg ataaagtaag ttttaatgat ggattagccc
    22861 gttatgattt gagaaaagaa ggcgccaaac atttccatca tcatttacta tgcttgaact
    22921 gcggcacgat cgaggaagta gaagaggatc ttttacttga agtggagcaa gtagtagaag
    22981 aacgttatca ttttttagta aaagatcatc ggctaacgtt ccatggaatc tgtcaggaat
    23041 gctatgaaaa agaaa
//
LOCUS       DAMYXM010000039        22991 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.040, whole genome shotgun sequence.
ACCESSION   DAMYXM010000039 DAMYXM010000000
VERSION     DAMYXM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.040"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(109..564)
                     /locus_tag="PNS21_001709"
     CDS             complement(109..564)
                     /locus_tag="PNS21_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159420.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="PNS21_001710"
     CDS             complement(718..1926)
                     /locus_tag="PNS21_001710"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDI5159421.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="PNS21_001711"
     CDS             complement(2038..2370)
                     /locus_tag="PNS21_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDI5159422.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="PNS21_001712"
     CDS             2518..3393
                     /locus_tag="PNS21_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDI5159423.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="PNS21_001713"
     CDS             complement(3475..4056)
                     /locus_tag="PNS21_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDI5159424.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="PNS21_001714"
     CDS             complement(4062..5321)
                     /locus_tag="PNS21_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDI5159425.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="PNS21_001715"
     CDS             complement(5323..6369)
                     /locus_tag="PNS21_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159426.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="PNS21_001716"
     CDS             complement(6604..6879)
                     /locus_tag="PNS21_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDI5159427.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /gene="der"
                     /locus_tag="PNS21_001717"
     CDS             complement(7122..8432)
                     /gene="der"
                     /locus_tag="PNS21_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDI5159428.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="PNS21_001718"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="PNS21_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDI5159429.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /locus_tag="PNS21_001719"
     CDS             complement(9978..10658)
                     /locus_tag="PNS21_001719"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDI5159430.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="PNS21_001720"
     CDS             complement(10707..11342)
                     /locus_tag="PNS21_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDI5159431.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="PNS21_001721"
     CDS             complement(11408..12811)
                     /locus_tag="PNS21_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDI5159432.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="PNS21_001722"
     CDS             complement(12798..13829)
                     /locus_tag="PNS21_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5159433.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="PNS21_001723"
     CDS             13860..14081
                     /locus_tag="PNS21_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDI5159434.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="PNS21_001724"
     CDS             complement(14246..14983)
                     /locus_tag="PNS21_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HDI5159435.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="PNS21_001725"
     CDS             complement(15136..16476)
                     /locus_tag="PNS21_001725"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDI5159436.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="PNS21_001726"
     CDS             complement(16713..18236)
                     /locus_tag="PNS21_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDI5159437.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18614..19225)
                     /locus_tag="PNS21_001727"
     CDS             complement(18614..19225)
                     /locus_tag="PNS21_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDI5159438.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19348..19473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19555..20271)
                     /locus_tag="PNS21_001728"
     CDS             complement(19555..20271)
                     /locus_tag="PNS21_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDI5159439.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="PNS21_001729"
     CDS             complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="PNS21_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDI5159440.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20830..21627)
                     /locus_tag="PNS21_001730"
     CDS             complement(20830..21627)
                     /locus_tag="PNS21_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDI5159441.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21597..21968)
                     /locus_tag="PNS21_001731"
     CDS             complement(21597..21968)
                     /locus_tag="PNS21_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI5159442.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="PNS21_001732"
     CDS             complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="PNS21_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDI5159443.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6446 a         4913 c         3899 g         7733 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccg ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    18301 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    18361 tttccctatt ccctcctctt ttttcagttt tttaaagctt tcttcttttt cattgtagcg
    18421 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    18481 caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa
    18541 cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg
    18601 atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac
    18661 ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt
    18721 tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca
    18781 tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg
    18841 cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc
    18901 ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg
    18961 ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca
    19021 gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca
    19081 tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga
    19141 tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca
    19201 ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa
    19261 ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag
    19321 ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt
    19381 atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg
    19441 gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca
    19501 ttatacatat ctattcaaaa aacgcaacat ctaacaatta taaaaagtaa gtgttcattt
    19561 tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg
    19621 taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc
    19681 aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac
    19741 gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt
    19801 tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc
    19861 ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc
    19921 aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac
    19981 aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc
    20041 atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc
    20101 ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa
    20161 ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt
    20221 tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat
    20281 tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt
    20341 tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag
    20401 tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca
    20461 atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc
    20521 tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg
    20581 gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct
    20641 tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag
    20701 ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga
    20761 tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg
    20821 gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat
    20881 tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa
    20941 agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt
    21001 acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt
    21061 tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat
    21121 tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc
    21181 agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc
    21241 tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt
    21301 gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtgc cgaattcttg
    21361 gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc
    21421 tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt
    21481 gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag
    21541 cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac
    21601 tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca
    21661 cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc
    21721 tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc
    21781 cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt
    21841 tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc
    21901 agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt
    21961 gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc
    22021 gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct
    22081 aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta
    22141 tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc
    22201 agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc
    22261 atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat
    22321 tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt
    22381 accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc
    22441 atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc
    22501 gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc
    22561 tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc
    22621 cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct
    22681 tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc
    22741 catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag
    22801 ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg
    22861 atttgctctt tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt
    22921 ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact
    22981 ggtttctttt t
//
LOCUS       DAMYXM010000040        22861 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.041, whole genome shotgun sequence.
ACCESSION   DAMYXM010000040 DAMYXM010000000
VERSION     DAMYXM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.041"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..870
                     /locus_tag="PNS21_001733"
     CDS             <1..870
                     /locus_tag="PNS21_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-rhamnosidase"
                     /protein_id="HDI5159444.1"
                     /translation="YGRAKDWIFIDWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAE
                     TYGENENYHTHLYQEKYQLLKQNLFDYFWDEELGAFIDCYESGRRNVTRHANIFAVLF
                     DFANKDQQQSILKNVLLNDTITQITTPYFKFYEQDALCKLGQTNIVYQTILDYWGGML
                     SHDAVTFWEEYDPTQTGNEKYSMYGDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGY
                     EEFMIQPNLDSFETLNCTLPIKDGKISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAK
                     QPLVLTSYSKINA"
     gene            complement(953..2326)
                     /locus_tag="PNS21_001734"
     CDS             complement(953..2326)
                     /locus_tag="PNS21_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDI5159445.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(2352..2636)
                     /locus_tag="PNS21_001735"
     CDS             complement(2352..2636)
                     /locus_tag="PNS21_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDI5159446.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(2638..4653)
                     /locus_tag="PNS21_001736"
     CDS             complement(2638..4653)
                     /locus_tag="PNS21_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDI5159447.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(4832..5137)
                     /locus_tag="PNS21_001737"
     CDS             complement(4832..5137)
                     /locus_tag="PNS21_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HDI5159448.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(5226..6257)
                     /locus_tag="PNS21_001738"
     CDS             complement(5226..6257)
                     /locus_tag="PNS21_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDI5159449.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            6464..7339
                     /locus_tag="PNS21_001739"
     CDS             6464..7339
                     /locus_tag="PNS21_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDI5159450.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(7491..8333)
                     /locus_tag="PNS21_001740"
     CDS             complement(7491..8333)
                     /locus_tag="PNS21_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDI5159451.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(8425..10302)
                     /locus_tag="PNS21_001741"
     CDS             complement(8425..10302)
                     /locus_tag="PNS21_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDI5159452.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVCNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(10299..11720)
                     /locus_tag="PNS21_001742"
     CDS             complement(10299..11720)
                     /locus_tag="PNS21_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDI5159453.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(11723..15112)
                     /locus_tag="PNS21_001743"
     CDS             complement(11723..15112)
                     /locus_tag="PNS21_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDI5159454.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            15369..16760
                     /locus_tag="PNS21_001744"
     CDS             15369..16760
                     /locus_tag="PNS21_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5159455.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(16833..17480)
                     /locus_tag="PNS21_001745"
     CDS             complement(16833..17480)
                     /locus_tag="PNS21_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDI5159456.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(17488..18174)
                     /locus_tag="PNS21_001746"
     CDS             complement(17488..18174)
                     /locus_tag="PNS21_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5159457.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(18180..19253)
                     /locus_tag="PNS21_001747"
     CDS             complement(18180..19253)
                     /locus_tag="PNS21_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5159458.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            19475..20149
                     /locus_tag="PNS21_001748"
     CDS             19475..20149
                     /locus_tag="PNS21_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI5159459.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            20154..21494
                     /locus_tag="PNS21_001749"
     CDS             20154..21494
                     /locus_tag="PNS21_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDI5159460.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(21643..22629)
                     /locus_tag="PNS21_001750"
     CDS             complement(21643..22629)
                     /locus_tag="PNS21_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HDI5159461.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         6644 a         4329 c         4158 g         7730 t
ORIGIN      
        1 tatgggagag caaaagattg gatatttatt gattggtctg aaatggataa agaaggaacg
       61 cttgctgcag aacaagtctt attactgaaa gcctatcatt ccatgatcac ttgcggagat
      121 atcttagctg aaacatatgg agagaacgaa aattatcata ctcatctcta tcaagaaaaa
      181 tatcaactat tgaaacaaaa cctatttgac tacttttggg atgaagaact tggcgctttt
      241 atcgattgct atgaatctgg gagaagaaat gtgacgcgtc atgcgaatat tttcgctgtt
      301 ttattcgatt ttgctaacaa agaccaacag caatcgatct taaagaatgt cttattgaat
      361 gacactatca cacagattac tactccatat ttcaaattct atgaacaaga tgctttatgc
      421 aaactcggac agacgaatat cgtctaccaa acgattcttg attattgggg agggatgtta
      481 tctcatgatg cggtaacttt ctgggaagaa tacgacccta ctcaaacagg aaatgaaaag
      541 tacagtatgt atggagatcc ttatggaaaa agcttatgcc atgcatgggg agcaagccca
      601 atctatcttt taggacgcta ttttatcggt ttgtatccaa caaagccagg ttatgaagaa
      661 tttatgatac aacctaatct agattcattt gaaacactga attgtacgct acctatcaaa
      721 gatggaaaga tttcgcttga aaaaacaaaa gataagatcg tgattacttc tgatagaaca
      781 ggcggaacag tgaaaatgaa tggtaaaagt tatcctcttc tagcaaaaca gccacttgtc
      841 ttaacaagct attcaaaaat aaatgcataa aacaaaaaac tgcttgcgac atatcagaag
      901 actttaaatc ttcttctgat attgtccaag caatttttat tttgaagttt gcttaattct
      961 gttctttaga aacaatgttt gttctttttc cggcaaaaag aaagctagat aatacgacaa
     1021 gtaaaaagaa agcgatgatt ccaactgtca aggctatttg accgtattct cctagtttcc
     1081 caagtacaag tcctggtact agataatcag tatcggcaaa tgtagaagaa gcgattttaa
     1141 gatctcctaa tactggaagg ataaataatg gcagccaact aatcaataag ctattgaaag
     1201 cagaacctaa taccgcccct cttctaccgc cagtcgagtt gccgaagaca ccagaagcag
     1261 ctccgcagaa gaaatgtcct actaccccag gaatgataac tgtagtttgc aagcctatca
     1321 tagcaaacat ggatagtgtt cctacaaaga agctaacaaa aaagccaatt aatacagcat
     1381 taggtgcata tggaaaaacg ataggaacat ctaatgctgg tttagaatcg ggtacgagtt
     1441 ttttagcaat cccttggaat gctggaacga tttctgccaa caccattcga accccttgaa
     1501 ggacgataac aaacccagct gtgaatgttc cagcttggat cagtgcaaag attacagcgt
     1561 tgtctccatt gcttagttct ctttcaacaa atccaggacc agctaacaaa gcaagtgcga
     1621 catatacgat cgacatcaag agcatgatcg atacagtaga atctcgcaaa aagctgaaac
     1681 ttttaggaat ctttatgtcc tctgtagaac ggtctttgtt tccaaaaaac tttccgatcc
     1741 atccactaga agcataaccg atatttccgg tatgccctaa cgcaacttga ttgtttccag
     1801 tgatgttctt cataaacggt tgacaaagag caggcgtaat agttaatagt gttccttcga
     1861 agaccccgcc taataagatc atccacaaat ttgcagtaaa ccccgcgctt actaaaatga
     1921 ctgctgttag acaagaaaca taaagcattg cttgacctgt aagaaaaatg aatttatatc
     1981 ttgtgaatct agcaagtaaa atattcacga tcatccctac tagcatgatc aaagcagtag
     2041 gtgtaccgta ttgttgcaaa gcaatcgcca ctactgcttc attgctagga actactcctt
     2101 gagtatgcag agctgtttgg aacatcgaag caaaaggggt tagtgaatta ctgattaaac
     2161 cagcaccgcc gatcagtact aggaatccga caaatgtttt gatagttcct tggatcactt
     2221 ctgaaaattt cttcttctga agaacgagac caagcagtgc aatcaaagaa acgaggatgg
     2281 agggctgact gacgattgac acaaacaaat tcaacaattt atccattgat agttcctccc
     2341 ttgaatcatt tttattcttt tgacaagatc ttcgttactt tttcttttac ttcttcgctg
     2401 tcaatgatcg attgcaaagg tacgagcttt tcatcaggta tagaagttag cgtatctttc
     2461 aaagaatgct caatgaagaa ataatcagcc atatcgctag atacacttcc aacatccgta
     2521 tgacttactt caaactcgtc cacatctgct ccaagatctg caagtgcttg tttgatgttc
     2581 atctctacca taaaacttgt tcctaatcca ctttgacata ctgcaaaaat tttcatatca
     2641 ttcgtctcct tttcttatga tcgctaatat ttcttctttt gttgatgcgt gtaataaatc
     2701 ttgaagattt tgtttattag aaagtagctt tgttaaattt gccaaagccc gaagatgtgc
     2761 ctcattatcg atagcagcta aacagatgaa caaactaatg ggatgggctg gattatcaag
     2821 cagatccaca ggttgttgaa gtttcagcaa agacatccct acttcgttta cgccatcttc
     2881 tggacgagca tgaggtaatg cgatgtgatc gccaatatgg ataaacgcac catactgctc
     2941 cacgcgctcg atcatagctt tgatataact ttcttctatt ttattttctg ataatagcgg
     3001 ctgtgcggca aatgtgattg cttcttgcca gttttttggt tgatccgata attggatcat
     3061 ttcttctgtt aataattcag atagcatagg acgttgatcc tccttctttt ctaaaagttt
     3121 gttcatttta cgatttaata cttgatagag cttttcttcg tctacgcctt cttttaattc
     3181 gatataaggc agtagcgccg acaacaattc ttgggcagaa ggcattgaaa atcctggtat
     3241 cagccaatct ctttgtactt gattgatcag ctgatttttt tccaagtcag tcatgattgg
     3301 atgcagaaca tagagagact ttttcttatc agtaagtgtg agaggtacag tagaaaaaat
     3361 aatatcgtaa gaagtttcgt caacctcgtg taagcggtca gctgaattag tctctgtaaa
     3421 aaagatcatc gggaataatt gctgaagttc actttttaag atcaatgaag agctaatgcc
     3481 acttggacag acgatcacgg ctcttatttt tctatttctt tcttctgcgt ccgcttttct
     3541 gatctctccg ccaaatagta tagtgaaaaa accaatctct tcttcaggga tgctcttccc
     3601 tattcgtctt tctaacggaa gtaatgcttt tcttgtcatt tcgtagatgg aagcatattg
     3661 atgcttaatt tgatcgatca tcacattagg taaataaaaa gaataggaaa tacgaaaata
     3721 ggcagggacc aagtgataaa agacattcat caataattca cgaaacgaag caaattctac
     3781 ggccatcagg aattccatgc gatggatgac ttctgaagaa cattccagca aaaaatcaag
     3841 tgcaggctcc tggacatctc cttcaacgat cgatgctaag caaagagaga aaaatataat
     3901 ctccgaattt tgatgttcgg aatagccaag gccagtcaat acttgtcgac taatgtcagt
     3961 gactgcaaga gaagccaaca gttcagcatc ttttaccagt tccatggatg tttttccaat
     4021 tcgaatggtt gtcaaactca aataataagc taattcatcc attcgatttg gtgcgaatcg
     4081 aagatctgat agtgaaatcg ttttatctag caaatctcta actttggcgt aataatcaat
     4141 ggaaagggtt gcggttaaat acgtgagccc aaatcttcca gcaagtgact gcatcaaacg
     4201 agcagtgcga ttttttgctt ctcttcttaa tattgcttct gaccctagca gcctaaaacc
     4261 agttttccta gtatacgaca gctgacaatc ttccacttct aattcatttc ttatcttttt
     4321 gatatctgac aggactgtcc ctttagaaac ctgaagaaaa tcttgaaaat gaaaaacaga
     4381 aacagacgtt gcagtcgaat aaaccatcaa ataaagaaga tctcttcgct cttgttcggt
     4441 atacacagct tcaaatcgag taatatccaa aaatttttct gcccagtcga ttgctcggaa
     4501 tttctttata cggatacggg tatcatccac atattcggca aaaggagtta attgtttgat
     4561 cgcctcgctt gtatttactt cgatgatctc tgctgctttt tctagactga tttctcttgg
     4621 tgttttcagg agataactaa ttttgtagtt cacagcgctg ttcacctcct acttatattc
     4681 tagtatggta gcgcttttaa atcgaggggt gtttggtaca acttacagtt ggattttttt
     4741 tacgactgta ccgaacgatt tctatcgaat ggatacagaa aaaaagcagg tgaccaaatg
     4801 tctcctgctt ttatgaattg atttcgtccg atcaatgatg atcatgttta ttttttgagg
     4861 tcagttcttc aaatttttct ttgacatctt caaccgcttt ttcgatatct ttttcaaagt
     4921 gattgggctc attaggcttt gtttcctctt ctacagaatt tgaaccagat aaagctgttt
     4981 ctttattttc tgtttcacca gagtttgtgc cagctaatga ttcttctttg tcttctgttt
     5041 caccagaatt agttcctgct aaagccactt cttcttctgt tttaggggcc attgaacctc
     5101 ttaatcgttt gtgtttttca ccgtcataat ttgtcatgtt ggttcctcct agtagttcta
     5161 tactttttca tcgtacaata agacagttta tgcgtcaaaa aacaaacccg attcttacaa
     5221 atatgtcata tttttccttc tgattcttga atgcttttga ttccttcttc caaaattttc
     5281 atttgtaaga ttgtttcttc atctttgatg actttttctg cgccattaac taatgtctcg
     5341 taaatccctt cgtagacacg gctgtaatca ccaacctcgg aaattacttt ttcttcatga
     5401 taactccctt gttcatctat ataggtcaat acaccataat gttcgggcaa atcgatacca
     5461 aagtcttcat gatcaggcat gtaaaacatt ttcagatgtt cttcttgtcg atcttttgtt
     5521 tgtttcacga acatcccttt ttttccgtaa acgacgaaac ttggacgttc tttgatgcgg
     5581 aaataactgg attttatcga aattttcata tttccataga aaaaatccag atcgaagtaa
     5641 tcattcattc gtcctttccc taaaagttga cgtacatcat agctgatatc atctggttcg
     5701 ccgaaataag aaagaacttg atctactgta tgacaagcat gtccatataa atagctgttc
     5761 gcagtagaaa attcatgcgt attttctggt acttccggtc tgaaataatc ataatgcatt
     5821 tctacttcta aaatatcccc taattttccg ctttcgatta ctttttggac tgtcaagaag
     5881 tcagaatcga accgtctgtt ttggtaacat tggataaata gacctttttc ttttgctaat
     5941 gcaaataact cttctgcttc ctttgacgtt tctgcaaaag gtttctctac tagtacattc
     6001 ttaccatgtt cgagtgccat ttttccatat tcataatgaa attgactagg tgtagtgatg
     6061 acaactaatt ggatctcagg atcatcccaa agatcagaca gctgatcagt atattcgatg
     6121 tcttcgaatg ttttccacac tggttcagat ggggaaaaaa tttttttgat attgattttg
     6181 ttcgtaagtt ttcttgaaaa aggcaaatga tagcgatttg tactctttcc attgccaaga
     6241 taagcgattg taagcatgag tggtgcctcc ttttattgat gttttcagta tatcctagaa
     6301 aagcgcagaa agtatttata atagctattc tggtaaaaaa gttcagaaaa acacttgtta
     6361 tcattcggtt tggtgaaatt aatgttcaaa gtaaggataa aaatcaaaaa gatagtagcg
     6421 gaaccaagtg tttatcagta agatagaatt tggaggtcag atcatgctta ttgaagaaaa
     6481 actaacaaaa caagagttgt ttaccacgac agagaaacgg attgtcgatt acatccgtag
     6541 gaatatcgaa gctgcagtct acatgacgat tgaagaatta gcaaaagcta cgtatacttc
     6601 ccattcagct atcattcgtt tatgcaaaaa aatgggattc agcggattta aagaattccg
     6661 tttcgagctg gggcgggaag ttcatcaatt gattgctcag ttcaatcaaa cgattgatcc
     6721 taattttcct ttttcaagtg aagatagcac tcagcaaatt gcaaagaaaa tggcggattt
     6781 atcgatccag tcaataaaaa aagcacaatt gcagattgaa catgaagatt tagatcaaat
     6841 cggtaaagta ttgaacaagg cacagcgaat ctttcttttt gcaaaaggtg attctcaaat
     6901 cacggcaagg aaattccaga ataaaatggt caagttgaat aagtttttga tcatggctga
     6961 agaatacagt gattcttctt ggaatgcagc caatttgact agcgaggatt gcgccatttt
     7021 tattagctac agcggtcgca tccatcatta tgaacgaata atgacctatt tgaagcatgt
     7081 agaggcccct accttgctga ttaccggaaa tcagcattca gaaatggcca aacaggctga
     7141 tatgtgtctt gttatatcgc aggaggaata tgattttgct aaagtagcga ctttctcctc
     7201 tcaaattgcc ttcgattacg tattaaatac gttattctca gtgatatatt ctcaaaattt
     7261 tgaagagaat atccgcaact tgaaaaacaa acaagaattg atgcaaaagg gccttcttaa
     7321 agaatatcgg aaaacttaag aaaagatcct catttagtaa taagaaagat agtgtttgat
     7381 aggctctctt tgatatcatc ataaaaacag gctgaagttt tctttttcaa acctcagcct
     7441 gtttgtttac ggaacgattc gaatagatat attatagaaa tgaggaatag ctaacttctg
     7501 tttcttattt tgaacgattg acgaaaaaga tatataagaa ggaggaaaat cagtatacat
     7561 gcagctaaca atccatacag gaaaaatcgt ttgttctcgg atactttctg tacttctttt
     7621 tgaacttctt ttgcatgata aggaacccgg tgccctctga caagtaagcg atgactgttg
     7681 accatataag gtgtacaagt taggagtgtc acgaaatcct gccctttttc gattctcaga
     7741 tcttttgtat cagtcggttc taccgtcttg atctgatcga cttgataagc gagtgtccgt
     7801 ccattgattt cgatatagaa agaatcacct aaagtcagtt tcggtaagtc tgtgaacaac
     7861 tttgcttgag gcagtcctcg atggctggaa ataacggcat gtgtattttc tccccccgta
     7921 ggataagacg ttccttccaa taatgaggca cctttttcta gcagcacagt attcgtttca
     7981 tcaaaaattg gcaaatggac attgatctca gggatcgtca aaataccgat cgtatgtttc
     8041 tcaaaatagg ttctatcctt tttcgcaggt tcttttttct tagtaaaggg atctgcacct
     8101 ggatttccac cttcttttgc taattgctgg tttttctttg tcatgttttc ttggatttta
     8161 gccatgactt cttcattttc tttgaccgct tgttgctgat aatgagagat gatctgctga
     8221 tccagataat tattcagcgc gtcgctaaca aatggataag ctagtatccc tattcctgag
     8281 agaagcaaga aaagcatcag cagatctaga agtttccttt tctttggttt catttgtgtt
     8341 ctccttgtga tgactgtttg ataataaaag aaaaaggagc atttgcgtaa tccgctgctc
     8401 ctttttccaa ttcttgcaaa aagactaaat ctgactgtgc ttgcgtctag caaagtacag
     8461 tccagcaatc agtagaagga ctactcctgc accgatatac acatagattc ccttaccgcc
     8521 tgttgaagga agtgtacctt tgtgtttatt tggtattttt tcaggagaaa tcaactgtcc
     8581 tgctgttaca taagattgtt catcgatagt aaatgctaca cggtttgtta atggaacata
     8641 tttttctggc gctttcgttt cttccagata gtacgtgcca tattttagac ctgtcacatc
     8701 gattaaaccg ttacttgtgg ttgtaaaaac cgttgctgat tctttcgccg atacccagct
     8761 gacggctttt gtggatgggt cgatcgataa atatttcgct gtgtcactat cttgatcacg
     8821 aacgacgaat tctgctccag caagtgtttg gtctgatgta acgtcaccat ctactttaac
     8881 aaatcgtttg cccccagtaa cgacatcgac tgacggtggt gtttgatcat ttgtatgccc
     8941 gttatcgaca tttgcttggt tagaaaatcc tttggttgga tctgcttttt cgttcaaatg
     9001 catatagtaa acgaatttca atgtaccgcc aggagttaat gaaggaatat aattcggatc
     9061 aactgaaacc gtgaatccgt ctgtttgctc agtgacagaa taatttactg ggtcgatttc
     9121 tttatttcca tcatataagg cataagcata cgttcctgaa gaatcattat caaatgttaa
     9181 agcagcgtca tgagtatcga taagtttgaa tgttgtgtac ttgttttgcg ttccttcttt
     9241 atcagcgata cctaatggga tattgacaga aatctcatat tgaatttttt caccgattgc
     9301 gacatctttt ccattcaatt gaggtgttgt tttatcaact ttagacgtat catttttgat
     9361 ggtcttttct actggtgttt ggctatttgc caggatctca aaaggcgtga ttgtttgctt
     9421 ttcaatcatt tccgcattat caggagcttt tacttcttct aaattatatt ttccaacttc
     9481 taaatgatta acgatcaact ggcctttttc aatagatgcc tcggcaaagt cattgttgcc
     9541 gatgtcataa gaatgaccag taatgaaatg tttggctttg gtttgatcag ttgtccaagt
     9601 gtacaatcca tctgtgacac tttggatgta ttttttgacg cttggtgttc cttcttcttt
     9661 agaaataata aattctgctc catttagtgc ttcgttttcg gcagtaccga tttttgtaac
     9721 tttcaacgtt ccatcattac agactgtatt tttagggtag agatggatag tatctagttc
     9781 ttctgtcccg tatttataag agccatctgc ttgtttgatc atctcatata cagggaaagc
     9841 taaaaccatg tttgcggcag ctgacactcc gtcttttggt tcttctttga tcgtgtagac
     9901 tgcatctttc ccattttgtt ttttaggtaa agataaagtg acatttccat cagcatctgt
     9961 cgttcctgaa gcaactggtg taccaggagt caaagactgg actgcttgtt ttgctgcatc
    10021 cacggacgct cctttatctc gttgcgcata aaattcttga gtgacgttat aaactgaaaa
    10081 tgaaatatcg gctaatcctt ggtattgatc gaattcgctc atttctttcc cgctgttttg
    10141 gattaaagga tcgggtaaat cagtcatttt ctttttatga aggatgactt gagctgctgt
    10201 ctcctctgca aacacttttt ttgcgccgaa gctgtttgtg agtaatggta aaataaggaa
    10261 aaggactcct aacataacgt ttattttttt atgatttttc attttttcac tcctgttctt
    10321 tttttgataa acaaccagat tgcaaagctt gcgaatacca taccaacaac gcctaaaagt
    10381 gtatttttcg tttcgtttgt ttttggaaga aatggacggc tattttcttt tttattgact
    10441 actttcaata tactgtccgc ctctttatcc tcagaaagaa aagttgaaat ggtaaacgga
    10501 atttctgttt cacttaaaat ataaccagaa ggtgcttcga tctcttttaa tcgataactt
    10561 cctgttttca acccggaaat tgaaaaagcg ccatttttat cggaaatcag gtgaagcgca
    10621 tctgattcgt tctttgtcca cttgtatccg ttggctgttt gctggaggta atttcctgct
    10681 ttgtttttaa taaggaatac tgcgccagct aatcgtttat ctggtttatt tgtctcgatt
    10741 ttgacaaatt gcttactccc tgtatataca gagactttct ttgtactgac accaaaacca
    10801 aaatccagtg ttccgttgtt gttgatttcc ttgttcgcct gtgctgtact gcttagctgc
    10861 atctgataag aaatggtcag tttttttcca gcatattttt ccaacgaagg gatcgaaaat
    10921 aaaacatcaa atccgtgagg tttcttgtgt atgacaaagc cttctgtcag cttttctcca
    10981 ttcgatgaaa tcgttaaact attaggtaaa aacgttaatg cttcatccgc actatctgac
    11041 aattcgaact tttgatagtc aaggatattt acaggaattg tcgtatgcaa ggaataagtg
    11101 attttttcac caatcgtaaa atcatttcta ggctcttcta ataccttctc aaaaggtgga
    11161 tcagggtagt cgttttcttc atttttagga tacaaatgga tagttgaaag tttttgattg
    11221 ttttgtccat gtacaggaag aacaactacc atattctctg ccttttcttt gacgacttct
    11281 ggtactttgg attcaatgaa aagatacgct ttatctcttt tttcttgatc tttagcggcc
    11341 agagaaaaag aagcgatacc atcttcgttg ttaagagttg tagttgttgc ttctgcggta
    11401 aacataccgg aagacgcacc gttttttgcg atctctgctt gtgcttcttc taccgttttg
    11461 cccttttccc gtagatggta aaaagaatct gtgacatcat aaacttggaa tgtgacacca
    11521 tttaatcctc gatacgtttg taataaagct ttttcttgtc cgtcatttgg atgatttttc
    11581 ggcagttgcc cgtttgggaa aagcaatttg tgtaaagtaa tctgaaccat ttctgtttct
    11641 gtttctgttg ctacctgagt aaaagctggt ttaaatagta acaagaagag acacatacta
    11701 agccaaccaa gttttttcat atttagctgc cccctctttc ttttttcaaa aaaagatacc
    11761 aaatagaaaa cgcacaacca atcatcccta ctagatagat tcctaaacgg ccaattcctc
    11821 ccgtttcagg taatggtact tttgcctgat tcgtgatgtc taaagaaatc tggttgtttt
    11881 tggctcctgg tgacagaaca gattcatgat cttgtccatc cacttgaatt gacccatctt
    11941 cgtgtataac tatagtaact ggctctttta gaccttggta tccttctggt gtaaaagttt
    12001 cggtcagcgt ataagttcca ggttttaaat tttcgaatag aaaggtatct gtttcttgtc
    12061 catcttttgg cgattctaag tccatttctg gtccctgcag tctgaatttt gctcctttta
    12121 atgtctgtcc gttgtcttct tttttgtgta ccgttagatc aaacgctttt agtcgatttt
    12181 gatgagtgag cgtccataca ttatttgtca tctcggtatt ttttcccgtg taaatgattt
    12241 ttccatattt atcgatttgg aattcataat ttcccggcga agtatcatat ccggcaggac
    12301 cagctgtttc aacgactcga tatttaccag gtgtgagtga atcaaaactg gcaagacctt
    12361 tttcattcgt tgtttggctg tcaattggct gataagtacc atttgcttct tttttctgta
    12421 gggaaaatgc tgctccttta agatttatct ctttgtccgt cccttgcatc gcatacttac
    12481 gtactgctac aggaacttca acaaaaggat tttcaatagt caactggatc gtatcatcgg
    12541 aagtagtgac tgtttttcca tcaatggtta ccgtaccatc agaagcgatc ttgatttccc
    12601 aagtagtctt tttgcttaat ccatgccctt ctggagcttt tgtttctgtc agcgtatagg
    12661 tcatttcttt ttgcaatttg acattttctg gaagagaata ggttccgtcg ccatgatctg
    12721 ttagtaaagt atcaatagaa tctcctgtta atttgaaaac agcgccaata agatcctttt
    12781 cacctgtaga ggaatttttc gttattttca agtttaacgg tgtgaattgt ttagtatttt
    12841 tccatgtcat tgcatctatt tgttgagaat cgtatcctgg tacaggtaat tctttgattg
    12901 tttggtaact gaatgcttga ccttgattat tgtattgagg tagtgataat atctcttgat
    12961 aactttctcc gctatttgca gataatttgt caatgtctgc acgttcccac gtatttgttg
    13021 tatctttagc tggtttaatg attcgaatat atccgttttt ccaagctgca gcatttgtcg
    13081 tatgttcccg ttgaatctca aaagtaactt gatctggacg atcagctaga ttgttgtcaa
    13141 attcttccca taactttttg atgtgaagac tgactccggg agctttagca gatggtatac
    13201 cgaattcagc taattcattg gtatcaatgt ttggctggaa agttgtcctg ccgttcattt
    13261 gataccagaa atttggatgg aagtcctcat tttctgtttg gattctcact tggtaatgga
    13321 tttggatttc ttggtctttt cctaaataga tctgattgct cttgatggta tttccttcta
    13381 tggaaataga tggagtgacc gttgtaggac ttgtccccac actcttgact gaaagtgtac
    13441 caggctgata aatgaatggt tctgcgattg gatcatttat ttgtccattg cttacagttg
    13501 ctgagatctg tacagctttt ttggctaggt attcagagat atctgttgca tgatcagctg
    13561 attcatagta aagatcgcct ttttcatctg atgaaaccat ttgtcgcata cgagactcta
    13621 cttctgcttt tgagagacca gctgccggat cgctttgaag ttggatgcca agaccatgta
    13681 tttcgattcc tcgttctttg agtgccatcg cttctccgat ggttgcgata aacgtactgt
    13741 cgattcttct ggatagattg ttttggtcag gtgcatcata gattcttgag attagagaag
    13801 tatttcccgg ccgatcttgc gtattagaaa actgagttcc gtaataattg ctgcttgatt
    13861 gtgcgtgtac ccgctgtact ttataggaaa atgttggtac accatccgtc agcaaaacga
    13921 tcactttttt atgtccattt ggaacggata gcatgcttcc tgcatctctt agtgcttttt
    13981 gtgtaaaagt accaccattt gtccgtgaag gtgtaatgga ttttacttga tttttcactg
    14041 aatcaaatga acccatctgt actgcaccgt tactgtagct atatccttcg cttgagtagc
    14101 cgacatatcc catattgatt ttgtctgtga taccgctatc tgctaaagta tcgacaaaac
    14161 gatcgacacc aatctttact tcaccgatcc gattattgtc gttcatactt cctgaccagt
    14221 ctacgaccaa tacgagatca agaggcgtga tatctttttg tacatttcca cgagcattca
    14281 aataaacatc aaacaatccg ggtgtacttg tttcctttgc atactttcgt atcgcatagt
    14341 ctgcttgatt accggttcct ccatattcga tatacgaatg ggtccgatca ttaggatctc
    14401 cattccaaga atttatacca tcccattgat ttgttccgtt tttattgcct tgatgatcaa
    14461 gaacatttgt atttcctgta ggttgccaac tggctttagg ataagtgccc tgatcatctg
    14521 ttgtatattc tgtaggaatc gtttgattag atggcacact cacagctggc aaggataatg
    14581 taggcaatgt aaatgggctg acagaatttt cgttttcttt tgatgattct tcttctattt
    14641 cgtctggctt tggtactgac acatctgtat ctttcttatt ttctgtctca tcaataatgt
    14701 catattcttt attatcgacg atcagcttgt ttgcctggct gtggtaaagt gctggatcga
    14761 ttggaaaact cagatcgata gtttgtttag tgtttgatga gatttccaac gtaattttcc
    14821 cgctatttac ctgataagca tagccatctc cttcttttct ttccacatca cttaagtaat
    14881 tttcgactag accaatttct ttttctatct tttcagcact ggtgttattc gcatttagct
    14941 tgatgttcag tgcttttttt ccagactgat ccgaaatgac ttcagctgaa attgtttcta
    15001 aaactgttgg atcatctgta gtgtctgcta ttgcagataa tggcacaaag tttgataaac
    15061 tcaatattat cagcatgaaa ataactttca gtttcttccc tgtggtcgtc atttttcccc
    15121 tcctttttct ttttttctgt tttgacgtga atagtgaaaa ttattaaaat aaacaggcgt
    15181 tacataattt tatttatttt aatatgataa ttattatacg aagggcgcta aagggaatca
    15241 caccaatttt cataccaatt ggtttcattt atgaagttga ttccttcgat taatgagcgt
    15301 ttcattgaaa gaaaagggtt gagggagaaa aacatatgat caggttgttg gaatgcaact
    15361 atagaaagat ggaaaaaatc attcgaattc tgtccatttc tgacaagtgg atgactaaaa
    15421 aagaacttgc caaacgaatt ggtagttcag aatctacatt tgtaaggtat atcgaagaga
    15481 tcaaaatccg ctggggaacg gatgtaacga ttcaatcttc acaaaaattg gggtatcgtc
    15541 ttgaacattt gaatgtacca gcttatttaa atgttctgat ggaaatgtcg caaagttcta
    15601 caaacagtcg tttattaaat gagattattg tcaatccggg taaaacgatt gagtattatt
    15661 gtgaaaaaat ttccatcagc cggtcttctt ttacacgtaa gttgaaacaa tgcaatcaaa
    15721 ttttagaaag ctatttatta aaagtgatcg ttgaccaagg ttttcagtta actagcctta
    15781 aaagtgagct ccagttgcgt atctttgtta cctttttctt tttaagttac tacggaagac
    15841 gcacgttgcc atttccaatc gaaaaaaaga agatcaaaga aattatggtc aagaataatt
    15901 gtcagatgaa tttgtttcag gaagaaagcc catatgagca atctttcttt attatgtatt
    15961 ttgttgtgca tctcatgaga gaatcccaag gaactccatt tgaatggaaa gctggagact
    16021 cttatgtaca gttgataaat atcgataaaa aagacttctt aatcttgaaa aatctcttag
    16081 gaaaattgaa gtacagcagc tatataaaag tagtgaacta ttttgtcaat acgatttttt
    16141 cagaattttc ggagaaagaa aaaaatgaaa tcagtgagca gatacgaaaa aatatgtcct
    16201 actgttcgtt ttcatccgct gattgcaaag aaaaaaacga agaatatgtt gttcaattat
    16261 taacgcaaat ttattttttc tcccgtattt tcccatacga tacgactcat ttgacgatcc
    16321 gtgcaactta ctttgcacag cagatgcaac accgccagat acgtctatat gctggatttc
    16381 gtgcagaact tcaattttat tccactctac tgaatcagaa tctgatttat tatttatcta
    16441 gctatatctt ttggatgctt atcggtaatc ctgaaattca ccattatact tctgataaaa
    16501 aaatactgat tatcagcgac ttaagtcttc gacatagtca atatattgaa gaatatatca
    16561 gtgatattct tgctgtgcat aaaattcact ctgagacaac tgctattact gaagatcagc
    16621 tgtcgaaata caatcttctc gagtatgatc tgatcgttac gaatcaaccc attttgaatg
    16681 cccatgttcc tcatatattg attgatgata gtgtgtcatt cgcaaatgaa gaagaactca
    16741 tccgcttatt cgagttatga ataatcaaaa aacagctatg tataaaccac cctgataaaa
    16801 atttcaggac aaatacacat agctgtttct ttttattgta ttccttcagc aatccaacga
    16861 gatttatagc gaaagtcttt taacaactct tcttttgtcc aattatttgc aatgagatcc
    16921 gtcaaagtgt tcattacgag ggtcatggaa aagtatagga aactaactgc ttcttccgac
    16981 gattgaatgg agaaatgatt gattcttaat aattccagcc atttttcgtt cattggagaa
    17041 tcagccgata gttctctacg tttggttgtc tgctgatcat ttcgctgggt taacaagtat
    17101 aacctttgcc aatagatact ttcgtgatct aagtcgcagc accactctaa atatttttcg
    17161 atccccaaaa agaaatgttg tttgttttcg ctaatgaatt ttaagatatc ttgatggact
    17221 atgtgagaac tgcttttgat cgtatacaaa taagcatctt ccaaatcttg aaaatattta
    17281 taaaaagcac cgcgcgacat ccccatcttg tcaacgattt ctgagacttt gacttggctg
    17341 atgtgctgtg agcaaaatat ctccagtaat aggtcatcga ttctctgttt ctttgcgttc
    17401 gataaattga aaaaagtttc ttttggcatc cacatctctc ctttatctct gattatacgt
    17461 ggatagccct tcattgtcat gaaggattca ttccttcttt ttctcttctt ctgtcaagta
    17521 cccatcttcc attcgataaa cgcgatcact ccattgaatc atacggacat catgagtaac
    17581 cataatagtc gctttttgtt tctcatgagc ttctttaact agtaatttga cgacttcata
    17641 tgcatgatct gtatccaaac tagctgttgg ttcgtctgct aagatcaagc ttggctcatt
    17701 atataaagcg cgagcaattg ccactctctg tctttctcca ccagaaaggt ctttaggata
    17761 actattttta agtgactgga tatctaaaga ttgcagcaag gaatcgattt ttgttgaatc
    17821 ttcttttttt gaagtaactt ggtcaactag ttcaaattgt tcctttaccg ttaaaaaagg
    17881 aatcaaatta gagctttgga gaataaatcc tatttcttta aagcgtaaat ctgctcgttt
    17941 tttttctgga agctcagtta gtgagtgacc gttgatcact atttctccgg aagtcggtgt
    18001 ttgcaatcct cctgaaatgg taaggaaagt acttttacct gaaccagaag gaccaatgat
    18061 gctgacgaat tctccttcct ttacataaaa atcaattgct ttcaaagctg agacctttgt
    18121 atgaccagaa ccaaatgttt tggtaacttt tttcatcgtt aatatttttt tcatttttat
    18181 taacctccta ttgctgtaat tgggtctact ttggttatcg tacgtatcga aaacaatccg
    18241 cctagcattg cgacaatgat caaaatccca ctatatagca accattgtga ccaaaaaaca
    18301 gcaaaaggca tcgcgcttgg taaaatcaga ctagtcaaat aagcaaacaa aagagcaagc
    18361 agaacgccta ccaccccaac aataaaggac tgtgctacta aagatttagc gatgaagaaa
    18421 tttttgatcc cttgtgcttt cattactcca aaaatagcag tcttctgaag tgtcatcacg
    18481 tacatgaaaa taccgaccac ggcagcaacc actaagaaca aaaagtatat catcgcattt
    18541 aaggtcatat tttgggcaga atagccagga atactttcaa tgaatgtccc aatagttaat
    18601 aactgtagac ccttcgatgc tttttctttc gaaattttag ctgaatcctt tattacgatc
    18661 ccattgatcg gctgttcttc ttcagaagca aacggctgtt gtccatactt caaagcagtc
    18721 cacgtatcta gggacgcata gatgacggga gaaactgtgt aataagtttc aggaaagata
    18781 ccgacaatcg tcaaaggatc gtcgtaactg ccaattttga tcgtatctcc aattttatag
    18841 cctgaatcag ccagattttg agagatgatg atttcattct ttgttttaaa ttcatgccct
    18901 ttcgtgagtt ttggtaataa aaatgcttct ttcgttgtgc cgaaaactgt aacattttct
    18961 ttgttttttc ctttgattgc tccgctgtac aatccgattg gtgctttctc tcctccttca
    19021 atgtatttca gatctcctct tgttaattgt gaagctgcaa atacttgatt tgcatcctcc
    19081 gacaaaacaa tctcttgtgc gtcccaatca tcgattgctt ttttaaactc ttctgaaagt
    19141 ccattcgcta gtcctgacaa catgaatacg acatacgcaa tcaagaacat aataccgacg
    19201 atcaagccat atcgtaattt agaatagcga atttctttga tagctagaaa catttgtttt
    19261 tcctccctat gatagattga tagtgacaaa atgtcacttg ttgaatagtg taactctaaa
    19321 gtgacaattt gtcacttgtg ttgttttttt cttatcaata aattgtctaa gacatctttc
    19381 gataaaacct taacatgagt cataaaaaat ttatttaagc cttatttatg ctttccatga
    19441 taaactaaag tgaccaaatc aatgaggtgg gaaaatgatt aaactattag tagtagaaga
    19501 cgataaagta ttatccaata atataaaaga aatactcgct gcaatcggcg agataacaca
    19561 agtttatgat ggtagtgaag gactgttcga gttggaaagt ggagtttatg atcttgcaat
    19621 cttggatttg atgatgccgg aaatgaatgg atatgaagtg ttagcaaaaa tacggaaaga
    19681 aaaaatacag actccagtat tgattttgac tgcaaaagat agtttggaag ataagattga
    19741 aggatttgaa cgaggtgcag atgattatct aacaaaacct ttctatcgag aagaactgat
    19801 catgcgcgtg aaagcattat tgaaacgctc gctaggtctc tttgatgaaa atgttctgaa
    19861 gtataaggat atcagttgtg atttaaaaag caatgaagtc ttgtataaag aacaagtttt
    19921 gcccatacaa ggaaaagagt ttgaattgct cgtttatttt ttacaaaata agggaatcat
    19981 tttgacaaaa gagcagatat ttgaccggat ctggggattt gattctgata cgacgattac
    20041 agttattgaa gtttatatga gcaacttacg aaaacatctg cgaagtactg gattggataa
    20101 agaaatcaaa accttacgta atgtcggtta tatactgcaa agggtgtgat gcaatgaatg
    20161 gagatattac acgaaagcaa cgtagtcgct tttttgcgac aaatgtagca gcatttgctt
    20221 ttatcttttt tcttttggga ttcattactt tacagatatt aaaccaatct gcatacagag
    20281 aaacagatct gtcattagag aaaataaatt ttgattctcg aacagtacaa ttagagatac
    20341 aaagatatca gcaaggcgat ccctttttaa acccgactca aacggaacaa aacagaacaa
    20401 acatggaaca gccattaatg gaaccatcaa atgaccgttt taacactcaa gtgatcttgt
    20461 ggtctaaaaa tggtgagata ttgaataaag cagctattgg tgggcgtttc aaccaaatca
    20521 aagaattaaa aatggatacg aaaaatctga ataccatcca aaatattgaa ttaaatgaag
    20581 acagtggctc atctgccctc tctttccgtt cgattacgaa aaaagcggag acgaatcaag
    20641 atggcgttgc ctacatccaa attttagaaa atacaaatca aatcaagaat tcattagcga
    20701 cttttcgcac gattatgatt ttgtgtatga ttgttttttg gattctctct attggtatca
    20761 gttattactt gtctagtcta agcatgcgcc ctattattct ttcttggaga agacaaaaag
    20821 aatttgttga gaatgcttct catgaattgc gcacaccgtt aacgattatt caaaatagtt
    20881 tggaacatct ttttaccaag cctaatcata ctattattga agaatccgaa agcatcgctc
    20941 aagcattgtc agagacgagg cgcctcactg gattgacttc ggatttgctg acaatcgctc
    21001 ggaatgattc tgatcagcag ctgctttcaa agcaaatgat caatacacaa gaatatatcg
    21061 aacaacttgt taaaccattt caagagatgg ccatcataga tggaaaagag tttattctcc
    21121 ataacgatgc aacattagaa gtaccaattg atgaaaaaaa aatccatcaa gttttggtta
    21181 ttttgctgga taatgcatta aaatatacga atccagaaga taagatcatt ttggattcat
    21241 ctgttaacca taaagaatgg ctgattcacg taaagaatac tggcccttct atcagtgaag
    21301 aagatcaagc aaggattttt gatcgatttt atcgggaaga tcaatctcgg gcgaaagaga
    21361 ctggcggata cgggcttggc ttggcaattg caaaacaggt aatcgaacag catcatggac
    21421 agatttctgt atccaattat cttccacaag gagtcgattt taaagtccaa ttgccgttga
    21481 agcctaaaaa ataatgctta tccctaaaaa agggctgtga caaaaatctt gtcacagccc
    21541 ttttttgttg cgaataaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt
    21601 ctttaagctg accacttctg tcacaacctc tttagcttac gcttacgatt ttttattgcg
    21661 gtcttcaatg tgtacatcac cgcttgtgtc aatatccaat tcttctcggc taacatcttc
    21721 tgtcactttt tcaacatctt catgttcttc tttatgaatc ccaacttctt cacgaataac
    21781 tgtatgtttg cttacatcta cttgctcttc tttgattggg atagtaatcg tgtcatcttc
    21841 caatttttcg tctgttccct gtgaagattg atctgttact ggtttgcgtt cgatgacgat
    21901 ttcttcacgt ttgaccggta cttctaccgt ttcagtatcg ttgaccacat gtttgtggat
    21961 gtcgacttct ccagttgtca catcatgtgt atttacatct aatttttctt cttttagttg
    22021 aattgtatca tcatctagat ttggtttgcg atcgatgtct tcagtcttgc ttgtacccat
    22081 tgtatcagtc tcgccaggta cgtccatcgg atcagttcct ggtgtcatat tcaatggatc
    22141 cgttgtattt tgaaagcctg tcgtgccacc tacgtccatt ggatctgttc cagtgccagt
    22201 ttctaatcca gtccccattg tgcttgctgg gctaacagta tcttgtgtat ccattgtatc
    22261 ttctgttgtt tctggctgac gatagccttt tactgtgatg acaaattttc catcagaaat
    22321 atctcctcta tattgatata aaggatcatt ttctggtgta gccgtctcag aatcataggt
    22381 atcaaaagaa aaagtatctt ttattttttc ccataaagaa cggtcttctt caccatccat
    22441 ccgattttca tctgtttcaa catctatacc agacaatccg tcaaatccta gtctagatgc
    22501 tgcttcatca gtagtataaa taacgatatc atctcgttca tacccttcgt tacgcaaccg
    22561 ttcaattact gataatgctt cttccctcgt actatagctt ccgatgaccg ttaaattttt
    22621 ctcttccatg taaattcctc ctagcaaata gatttatgat tggtatattg ttacttataa
    22681 atttattcta taggagacga ttgttttcta caaatattta gattggaatt tgattaaaac
    22741 aatttaattg aaatttgaaa acatttatgt taatgaaagt catattctaa taaaatgtca
    22801 tttgtttcta tatgaatttt tatgaaaaat ttcatataat ttccactcgt tgtgatggtt
    22861 t
//
LOCUS       DAMYXM010000041        22304 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.042, whole genome shotgun sequence.
ACCESSION   DAMYXM010000041 DAMYXM010000000
VERSION     DAMYXM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.042"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            2..400
                     /locus_tag="PNS21_001751"
     CDS             2..400
                     /locus_tag="PNS21_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDI5159462.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="PNS21_001752"
     CDS             384..1079
                     /locus_tag="PNS21_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDI5159463.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1673..2560)
                     /locus_tag="PNS21_001753"
     CDS             complement(1673..2560)
                     /locus_tag="PNS21_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDI5159464.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2745..3584
                     /locus_tag="PNS21_001754"
     CDS             2745..3584
                     /locus_tag="PNS21_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDI5159465.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3780..4754
                     /gene="sgrA"
                     /locus_tag="PNS21_001755"
     CDS             3780..4754
                     /gene="sgrA"
                     /locus_tag="PNS21_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HDI5159466.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5052..5408
                     /locus_tag="PNS21_001756"
     CDS             5052..5408
                     /locus_tag="PNS21_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159467.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5411..6568
                     /locus_tag="PNS21_001757"
     CDS             5411..6568
                     /locus_tag="PNS21_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5159468.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6594..7034)
                     /locus_tag="PNS21_001758"
     CDS             complement(6594..7034)
                     /locus_tag="PNS21_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDI5159469.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7194..7700
                     /locus_tag="PNS21_001759"
     CDS             7194..7700
                     /locus_tag="PNS21_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDI5159470.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7868..9780
                     /locus_tag="PNS21_001760"
                     /pseudo
     CDS             7868..9780
                     /locus_tag="PNS21_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
     gene            9785..10786
                     /locus_tag="PNS21_001761"
     CDS             9785..10786
                     /locus_tag="PNS21_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI5159471.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10988..11194)
                     /locus_tag="PNS21_001762"
     CDS             complement(10988..11194)
                     /locus_tag="PNS21_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HDI5159472.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11840..12688)
                     /locus_tag="PNS21_001763"
     CDS             complement(11840..12688)
                     /locus_tag="PNS21_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDI5159473.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12687..13754
                     /gene="gnd"
                     /locus_tag="PNS21_001764"
     CDS             12687..13754
                     /gene="gnd"
                     /locus_tag="PNS21_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HDI5159474.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            13756..14757
                     /locus_tag="PNS21_001765"
     CDS             13756..14757
                     /locus_tag="PNS21_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDI5159475.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14754..15038
                     /locus_tag="PNS21_001766"
     CDS             14754..15038
                     /locus_tag="PNS21_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDI5159476.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15071..15868
                     /locus_tag="PNS21_001767"
     CDS             15071..15868
                     /locus_tag="PNS21_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDI5159477.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15882..16715
                     /locus_tag="PNS21_001768"
     CDS             15882..16715
                     /locus_tag="PNS21_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDI5159478.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16733..17590
                     /locus_tag="PNS21_001769"
     CDS             16733..17590
                     /locus_tag="PNS21_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDI5159479.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17612..18121
                     /locus_tag="PNS21_001770"
     CDS             17612..18121
                     /locus_tag="PNS21_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDI5159480.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18158..18559
                     /locus_tag="PNS21_001771"
     CDS             18158..18559
                     /locus_tag="PNS21_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159481.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18671..19024
                     /locus_tag="PNS21_001772"
     CDS             18671..19024
                     /locus_tag="PNS21_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159482.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19058..19501
                     /locus_tag="PNS21_001773"
     CDS             19058..19501
                     /locus_tag="PNS21_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDI5159483.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19518..20603
                     /gene="uxuA"
                     /locus_tag="PNS21_001774"
     CDS             19518..20603
                     /gene="uxuA"
                     /locus_tag="PNS21_001774"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDI5159484.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20596..21309
                     /locus_tag="PNS21_001775"
     CDS             20596..21309
                     /locus_tag="PNS21_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDI5159485.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(21516..21665)
                     /locus_tag="PNS21_001776"
     CDS             complement(21516..21665)
                     /locus_tag="PNS21_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159486.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            22061..22261
                     /locus_tag="PNS21_001777"
     CDS             22061..22261
                     /locus_tag="PNS21_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDI5159487.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7679 a         3429 c         4622 g         6574 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattacta actattactt atttctcatt
     1201 taaacgatgt attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt
     1261 gaataaattt cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct
     1321 agtataacag aataagaaga acaagtccag aagggatatt tttagtcttt tttaaaataa
     1381 cttttcagtc gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa
     1441 atagggaagc atgaaagaat ctacttaatg gcgcattgat tcatgtgata gaaataaaaa
     1501 acttttttaa attatatcct catgattgta acaaatccag tgatttgttt tggtacaaat
     1561 aaaaaaaggc cccgatgaag ttgtggcaaa agtggtttgc ctcaaaaata agaaggaatt
     1621 tccaaaaatc acttctcaaa ttttgccaca tccccaattg ggtcaaattt gtttatttga
     1681 atatattttt tacataagga tctttaatag ttacattgtc ttttttcatc actttacgga
     1741 ttgttttatc catgaattcc gtatcaagag tttttgcttc ggtagcaatt ttttctagct
     1801 cagatttgta tttgtcttta ttagaacctt tatcctgttt ttttaccatt tttactagat
     1861 aatagctttg ctgataagtc gttggatctg ttacaggaat gatatcagaa acttccccgt
     1921 tttttagttt aaatgctgct ttttttactt cagtaggaat ctctggattg gttgaatcaa
     1981 aattcatttt tccatcagtt tcctttaatg ttttattttt tccataagct tgaacaagct
     2041 tggagaaatt ttctccttca tttgcttctt tttttgcttt atctgcttct tctttagtcg
     2101 aaaatagaat gatctgtgta ctaacttctg gatagaattc tttccaagca gtgtctagat
     2161 ctttatctgt taattttaca ttggctttca aaccgtattg gaaagccagc atctgtttaa
     2221 agagtttctt atatgtctct tctgtttggc caactgcttt taattgttcc ttgtagtttg
     2281 gaccgagttt tttgatttgt tcattatatt cttttgttac ttcctttgaa gaaatctctt
     2341 ttccgtatac atcatcaaat gctttgaaga caatcatttt ttttaatagt gtttgattat
     2401 ttgatttaac ttttgggtta tcataaaaat cctgaactgt aagtgttcca cctttaaacg
     2461 ttgcaatcgt ttcattggac ttgctacaac ctgagaatag taaaattgca catgctgaaa
     2521 ggcacataat tagtagttgt tttttcaaaa aatttcccat cctttcctta ttaaaaacag
     2581 tctaacagaa gaattaggga gacagtcttt cattttcgta aggttgctgt agatattctt
     2641 agttcgtaaa aatctattct ctctatgatg gagaacattt acatagtttt cacgagacaa
     2701 gaaagaaaag ggttgttaaa cttaatataa aggagggatg aaagatgatc aggattattc
     2761 ggttgattgg atctgtttta atagtcttag ttggttttgt tttaggaatt cttgtcaata
     2821 atgcacttgc ggatatgttt attggaaata gtgaatggat tggagcaatt gccggtacgg
     2881 tcggacattt agctgttttt ttactagctt tatttcttgc tagtaaggtc gaaggaaaaa
     2941 gaatagaaga ttatggtgtt tctgttcgag gaaaagactg ggggtattta gcgggaggtc
     3001 tcgttgttgg aattggagtg tttctgctta ttactagtcc attatatttg acgggggcgt
     3061 atcggttaga ttcaggaaac gcaaacattg tgccgctgat aacgagtttt attttattta
     3121 ttgcagtggg ggcatcagaa gagcttttat tccgtggctt ttttcagcat cagatgctta
     3181 gatttggtcc gttaatagca atgattggat ctgctgcatt atttgctctt ttgcacggat
     3241 tgaatcctaa catgacattt ctggcagttt tcaatatctt tttagcaggt tgctttttca
     3301 gcgcacttat ctacagtaca ggaagtttat ttacggctat tggtgctcat attacttgga
     3361 attggacaca aggagctatt ttgggtatac cagtcagcgg aacgcaagaa caaggttttt
     3421 tctccactct gatccaatcg caaaatacaa taattactgg gggaaacttt ggggctgaag
     3481 catctatatc aactacatta ttcttgttag tcttgattgt tggtttgtta ctctatgcat
     3541 ataaaacaaa gcggatgaag aaatacactg ctgtttatca ataaaagatg aagaaagcaa
     3601 ggctgatatc ggtcttgctt ttttaagtct gtcattgatt aaacgaaaaa gtaaacaaat
     3661 tcaagcgaat acacgacatc ttttgttaga ttttcacaac tgactgtcgt gttctcatca
     3721 ttttcatttt atataattcc tttatcaaat agaaggtaat tatatggaga gtgatagaaa
     3781 tgaagaaaac agcgaccgta ttatttgtta ttcttgggct gagtataatt gcagcaatga
     3841 ttggaatcag tacttctgca aatgaacggg caaatgagaa tctttcattt actgtgaaaa
     3901 ctgatcggat tgtttatgac atgacacaac aagtcattac tattccagtc aaaccaaaca
     3961 aaagcgtgaa tgctagtgat gtacatgctg ttctaacata tggttgggat ggtaatggaa
     4021 gttcagaaaa agttattggg gaagtttatt taaaagacgt acaatggacc gctggtattg
     4081 agtatacaat aatgatttct gcagaactat cgattgatga gatcaagagt aaagataaag
     4141 ttgatttgat tgttttttat gatggacaga tgacgatcac ggagaatttg aaaccaagca
     4201 gttggacagt tgttggaccg ccaagcagta cggatagcac aagtagtgaa agcagtacag
     4261 aaaacacgag cggtgaaagc agtacagaga gtacgagcag tgaaagtagc acagaaaaca
     4321 cgagcagcga aagcagtacg gagagtacga gtagtgaaag tagcacagga agcacgagca
     4381 gcgaaagcag tacggagagt acgagtagtg aaagtagcac aggaagcatg agtagtgaga
     4441 gtagcacagg aagcacgagc agcgaaagca gcacaggaaa agtaaataat gaacttgatt
     4501 tggtacctat cataccaact aaggaacaaa agagaaatgc agggagcaac ctaaaaagtg
     4561 tgctaaaaca gcctgatttt gtttctcgtg gaattgatca tcaagaaaaa gcgaaagaaa
     4621 cagataatag aaaaagcgat ttaccagaaa ctggtagtag aaccttgaac aagctaatta
     4681 catggatggg tgtgttactc attttgattg taggagcaag ctattttcgt tctctcttcc
     4741 atcgagtgaa ataaagaata aaagcaacag atctaaaatt gaataaaacg atagattttt
     4801 gagtgaatat ttcattcgag agtgctgtat tgcctggcga agttgtagct ttgccagatc
     4861 tatttttgtt tactaacata tttgagaaac aggaaataac aggaacacaa atggaaaata
     4921 agagcaagaa taattgacaa tgcacactgc gcatggtagt atctctgaaa aatcagtaac
     4981 gaatctattt gtacgttatt catgtagctg tcggaataag tctaaataat agataaaaag
     5041 gagtatgtac tttgtttgaa gaatttattg atatcaatga aaggcaagta tatcaatttt
     5101 tgaactattg ttatgaaaga gacgagaaat tatatgtggt gaaggatatc gctttagatc
     5161 taaactacac cctggctaag atgaattcgg tgattcaaca agcagaatcg ttttgcgaaa
     5221 ggtatccaga atataaatta tcttttcttt cagaaaacaa gatgataaaa gtcgagtttt
     5281 cttctcagtt tttactgagt aaagtttatt ctatactttt agagggaacg attggttata
     5341 ttttactgga ttcactttac aaaggtactt atcaatcctt agaaaatctt agtcaaaaaa
     5401 ttatataagt ttgcgtacga ttcaaagaaa attaagagaa cttaatacta ttttgaaaaa
     5461 ctatcattta tcttgcagct tgaaaaaagg taatccgata gaaggagaag aataccgtat
     5521 ccgatacttt tttcacttga tgtattggca gtcattcgac gaaaatcatt ctgatatatt
     5581 atcaagtata aaaaataaac gtcaactatt agaaaaattt gcgaaacatg cgccatatac
     5641 gcgtagtata gatagaaaga aatggatcag cctactaaga atttcgttgc aacgaatgaa
     5701 acaaggccac tcgattacgt atgtcccaga agagatgaac aaatttgtgc atccattgat
     5761 aacaaaagaa gagtttacgc attacatact agagcctttt tttgaagcaa acttctggtc
     5821 gatgaatcac atcactccaa cagagcaagc ttacctttat tttatgtttg gtgtaatgaa
     5881 cacctatttg gaagaagatt tgttggatac tccagataaa gtacaagaaa gttggggctg
     5941 gattgaatcg tctattgtag aaaattttga aaaaaacttc aagatttgtt ttacaacttc
     6001 agaaaaaaat tatctatatg tcaatttggc aatgatccat ttgtattcaa gggtttttgg
     6061 gacaaaaaag aaggtggatg cttttggaaa atcagcagaa gacagagatt ttcagcatat
     6121 atttccagtg atgtatccaa tcatgcaaca gttttttagt ggggtagctc gcagaattcc
     6181 agagcttaat acttattatg aaaagaacag acggttgatt tttcaatact gtatgttagt
     6241 cagggatatt tttataaaac acgaacaacc tgtcatattc tatattcaat caaagtatgg
     6301 taaaacacag gaattatggg caaagaaaag gatattatct atgactgaac gcccaataaa
     6361 atatagtgca tccgctgatc agttacctga tattgtcatt agcgattatc ctatcgacaa
     6421 aaaggcatat gatcaaacga atacacagat attttactgg aatggacaac ctacaaaaaa
     6481 tgattgggcg cgtctgaaag ctgaaatcat aaaaatcagg aatgagaaaa atagatccat
     6541 attggaaaaa ttggatttcg ttaattaaga aaagcagatt gtttatgcca aaattaagac
     6601 attgcaaaaa gattattttc tttaaaatcg acaagatgac cagaaagcaa gagttcgttg
     6661 agctcttgct ttcttttagt tttagaaagg aacatcctga ttttatcttc gctaaatcca
     6721 gtcaaaagtt ttgcagattg gtcgtagata atcggttttt tcaataaaga aggggatgta
     6781 cgaatctcaa tatatagttc actcaaactg agttcatcta ttctgtgaac aagattttta
     6841 taactaatag aatgagtaga gagcatttcg gtcacgccgt tgtttgtcaa ttttagtaag
     6901 tggataaatt catttttggt tagtggttga cgaattaagt tctgttcgat aaacggtata
     6961 tcatggacga taagccaacg ttttgcgcgt cttgaagacg agcagccaga acctgtatac
     7021 aatttaatca tcatacattc ctccaatagc taagtattta tgaaataata acatgaagaa
     7081 atagtcgaaa agtgccattt agaggtgtaa aataagaaaa tgttgtcacg tataatggaa
     7141 ctagctagat aagaaatact attaaatcaa gaaaaaagaa agaaggcaca agtatgtcaa
     7201 acacagccga cgcaatagta gtgatcgatt tacaaaaagg ggtatgtttt gattcttcgc
     7261 cgatacacga attagaaaca gtgacagcat tagtgaataa aagaatcaaa acatacgaaa
     7321 cgattgggaa accagttatt ttcgttcaac acgaagatgc tcaattagtg ccacaaacaa
     7381 aaccatggga gctattgtcg gaattatcta ttcctgagca cagttatttt gtgagaaaaa
     7441 cccatgcgaa ttcattttat aagactaatt tgagtgtttt attaactgaa ttggactgtc
     7501 attcgatcga attatgtggg gcacaagcag agtattgtat agatacgact gtgaagtttg
     7561 ctcatggatt aggataccag cttatgatgt gtagaggagc aacgacaact tatgataatc
     7621 cttttatgaa agcaaaggaa acaatcgctt tttacgagaa aatttgggat aaacgatttc
     7681 tgacattttt ggagcaataa gttgtttctt cgtttaaaaa aagcagaatg gatactggtt
     7741 tatccattct gctttattac gtaaaatgag aaaataatcc tgttctattt ttatgggata
     7801 aaccatttat tttttgtata aaaaatgttt gcgtttacat ataattctcc tagaaacata
     7861 aaaaggagtg aatagcttga gaacaaaaca agtattaaag ggagcgttgg tcatcttatt
     7921 tgctgtaagt acaagcgtcc cgcaatggac agtatatgga gagagcattt cttccgaatc
     7981 ggaatctcgg gaggtagaaa ccacagaaac tactgatgct ggaaatgaaa cgaaatcgag
     8041 tacttcacaa acagaaacaa atactgaaac aacggttgag acatctgtta ctagcacaga
     8101 tcaagaaacg accgctacca cagataacca acaaacagaa gaagcaacag ctacaaaaat
     8161 gtcaaacgac gaatcctctt ctgctaaagt aagtgacaaa gagaatggga cagaaaatga
     8221 tgatatcggt aaaggtgaaa atttcaatgg taaagaatat ttgaattact tcggtaaaca
     8281 gatgacagaa atacttaatg gaccaacaga agacatcaag tcaaaattaa tagagttatt
     8341 agtcactgag cttgctgtta atgattatgg tgtagataaa gagattatca aacatctgat
     8401 tgctacttat ctattgtacg atatcgcttt tgcaaaaaac agtgtgttga tgtcgatgct
     8461 tggaggtaca gatgatggga caacgcctac agaaaacaca ggcttctatc caaatacaaa
     8521 acaacttcat caaactgtac taaacaaaca agatggcaaa atgatggatg tatatgcata
     8581 ttatgtcgat caaggttcag ataaaacagt catgatccat ggcggatttc gagggaattg
     8641 gaataacggg atcgttacag aagaatacaa tgatttttat aaggccgggt ataatttatt
     8701 atttgttgat tccagagcga ctggtaatag cggaggcgat tatgtgacct atggacaata
     8761 tgagtcagat gacgtccttt actggataaa tcaagaagtt cgagagcggc catctcaaaa
     8821 gattttactt tatggtggtt cgatgggagc agctacgatg atgtctgtat tagcgaaaga
     8881 tattcctgtc aatgtcaaag gaattattga aaattgcgga tttgcatcaa ttgatgaaca
     8941 actacgtttt acgtacagcc agacagtagc ccccgcacta ccggatgcca taaaaaatca
     9001 gttagacatc attggggatc aagagcatga agatttattt atgggccttt tgaaacaata
     9061 ctattttgat caggagatgc atttggatac aaaagctgct ctacctacga ttggaatgtc
     9121 agattcattg cctaaattga ttatccatgg aacagcagat gatgtagtac ctgtgtctaa
     9181 cgcgcaaaaa ttatatgagt tatcaggagg gtataaagat cttttattag tagaaggagc
     9241 agggcatgga aaggcacagg aagttgacca tgctgcctac acgaaacatg taacggactt
     9301 tttgaaagta gtgttccatg atcagataaa cgtaaagtat gtggatgaaa acaataaaag
     9361 tctactcaaa gaccaggacg aaatccagct ttacggagct tatggagaaa attatatgac
     9421 agagcaaaag acattcgaag gatacgaatt agcaaatgtt gaaggaccga ctgaagggat
     9481 ttttaatgag acgataccaa ctattatttt ctattacaaa aaaatcccag tagctccacc
     9541 aaaaaagcaa gatccgacag agaatgcaga caataaggga aagaatgaac ctcgagagga
     9601 tcaatcaagt gatgatacgc aaccgattca gatgacagtg aaagtacaaa aaaagacaga
     9661 tgcaaaaaaa gaatgttgcc gaaaactgga gaaaagaaac attcgcttct catttattca
     9721 ggtggatttt tatctgttag cacgattatc acatggattt ggcgaagaaa gagagggtaa
     9781 atgaatgaaa tggttcaagc gaatagtgat tgcagtacca gttatcatcg tagttatttt
     9841 agtcactgta tttttgatta atcaaatcac accaaagccg attagcttgc ttgttagaaa
     9901 acaatttgac acaagtgaaa aagaacaagt atatgcccga ccagatgatt tccagcaaaa
     9961 aatcgaacaa atcgatataa aaaaagaaat atcctatcct tcaaagtaca aaaataataa
    10021 tatggatatc tacacaccga aagtggaaaa gaaaaaatta ccgattcttt tctggatgca
    10081 tggaggcgca tatgtaggag gggataaaaa tgattgtcgt gattatttgg aatatctatg
    10141 ttcagacacg cagcaaatca tcgtaaatat agattatgaa cgatcaccgg aagcaaaaca
    10201 tcctacacca gttatccaat tgaacgaagc cattcaagca gctaaaaaag agataaaaga
    10261 tcaagcagat tggtcgaata tttcgattgg tggaaattcg gcaggtgcac aaattagtgg
    10321 agaatatttg ttagcacttc aaaatgaaga aattaaaaaa gctgatcatc ttgatccgac
    10381 tttggagaac aagcagatta cgaagtttat ttctttgtct ggtctgttgg atccttctgc
    10441 acttactcaa gtaagtgata aaatctctag ctttctttat gaaaaatgtg gatgggctta
    10501 ctttgataat aaggactttg aacaatcaga gaaggtcaaa agtttagcct tggtcagaca
    10561 tgcggatcgt tggacgcaaa acacattctt aacggatgga aatacaaata cgtttacgaa
    10621 acaaatggaa gaagtaactt ctgtatttga gaaagcgggg gtgaaagtca caaaagtaga
    10681 ttatgcggag aaaaatgctg ttttgaacca tgaatatcag tttgatttca gcaataaaca
    10741 agcgcaagaa acatatcaaa agttagtaat gtttttcaag gagtaaagtc ttatgatgca
    10801 aaagaggttg taatctgtca cttagctcca agtcacaagg agcaatttca ataaacaagc
    10861 ttttcatggt ttatgaatat tgtaacttgt gactgaggag ttagagatgg caaggggcac
    10921 cttttattcg aaatttggga atatcacacg attattgtca cgatctcttt tacgtaaatt
    10981 ttataagtca gatttgttgt tttgatcgtt taaaaaaagc aacaaatccg aagcctgcgg
    11041 aggcaagaag ggacaaaaag ccgagtggta agcaaaagaa tgctggttcc actaatattc
    11101 cgttgttatt gatataaggc tgggaacaaa gatgaatagc aaataaaatc aatgcactag
    11161 cgaaaaaaag caaagaaata tggatagctt tcataagcag cacctccttt tttataattg
    11221 tatgataaat agattaagaa tctataaatg tgacaagttt gttaggatgt gaataaaatg
    11281 atcatctagc gaacttgaaa caaagatggc taatgataag caattaatga ttcagcaatc
    11341 agataggtac aaagtttacc atcatacgtc tagaccaata atagaaaata tgggaatata
    11401 atagaaaaga tttaggatac ccccaaaaga atttccccaa aaattcgatg tatcctaaac
    11461 ttttttattt tacataacaa tcatatcaac tgtgaaataa aattacaaat gataacctaa
    11521 aatgaggcca aaagccagtg ttatcaagga aatacttaat tcaccaaaat tttttttaat
    11581 tttttagaaa ataagaaaca aaaataataa aaaaaagaaa agataaattt tttactactg
    11641 tatactaact ttgttatcgc atctaacaag taactcgaat aataccaata caaccttctc
    11701 caaagatgcg atgacatacg ttctaaccca ccgactattg atgttctgta ccacccttgc
    11761 agaaccaaaa aagccactac caaatatttg gtagtggctt tttttgtttt atggattttt
    11821 cgctttttct gttatggtgc tattctttga tttcagaaat agtatgtcga actttagcga
    11881 tagaacgacg tgattctttt tcgttgtaaa acatcaaaat cacatataga gagtccaaaa
    11941 tgcttagttg agaaatacgg gaagctaaag cttcagatcg gaattctgtt tcttcagaaa
    12001 tagaaataaa aacgacatcg ccaagtttag caagggaaga attagcttgg ctagtaataa
    12061 cgattgtttt tgctccagtt tttcttacgg tttcagcaat atggattgtt tcttttgatt
    12121 gccctgtgtg agaaatcaaa acagcacagt ctttttccgt caataaagaa gcttccatca
    12181 attgaatatg gtaatccgtg ctgtgagaag ttgcgatagg ggaacgcaaa aatttatgat
    12241 aagcgtcagt tgctacaatc tctgaaccgc caacaccgaa aaaatagaca cgtttagatt
    12301 cattgataat agtagccgct ttttttagat ccttttgttg taacaacttt tttgtgtcat
    12361 ttaaagttga aatactagac tcgaacacct tttgcgccat agtgagctca gtatctgttt
    12421 tctgaatatt ttcgtggata gaaatagcag atagatcatt ttcttggatc aacatggcca
    12481 ttttaaaatc tttaaatcca ttataaccaa gtttttttgt aaattgaaaa aaagtagagt
    12541 cagcaatgcc cagttcactt gctaaattgc taataggact atgacttact ttgcttggat
    12601 tttctaaaat ataatcggcg atagcctgtt cttttgggct gaagtcgttg tacattgttt
    12661 tgatttgtag tcttatggag acgctcatga gaattcctca ttttcataat aagattatca
    12721 ttttttccgt tctcattaat gttaaagata caaggagagt aggaaaaaat caaacaaaaa
    12781 atatttttgt aaaatctatt tacaaaaaaa tttttttgta taatgagggt ataagaaatg
    12841 gaggcgctat catggaaatt ggtgtaattg gtttgggaaa aatgggattg aatttagctt
    12901 taaacttaaa agagcaccat cacaaagtac aaggactcga catttcaagt acagctgtta
    12961 gtcaggcaag agaaaatgac attcctgctt atcaagagat ctctgaattt ctagctactt
    13021 ttgagcagaa aaaaatcatc tggttaatgc ttcctgctgg agagataacc gaaaacatgt
    13081 taagaaaact ttttccaaaa ctcggaccag gagacattat tattgaaggc ggaaactctt
    13141 attataaaga ttcaatccgt cgagcagaag agtttcagaa aaatgaaatc ggttacttgg
    13201 attgtgggac ttctggagga atcgaaggag cacgacatca agcctgctta atgattggag
    13261 gagataaaga aacttttcta tccgtggaac aattattcaa agatgtagca attgaaagag
    13321 gatatctgta tgctggtgca tccggaagcg gacacttctt gaaaatgatc cataatggaa
    13381 tcgaatatgg aatgatgcaa gcaatcggtg aaggattcca acttgtacaa aaaagtgagt
    13441 atgcttttga cttggaacaa gtcgcacgtg tttggaatca tggttcggtg attcgcggct
    13501 ggctgatgga aatcatggaa gatcagttaa acgaacatcc caatttaagt aattatcggg
    13561 gaatcgtttc tgcatcaggt gaagcaaaat ggacggttga aacagcgcta gaaatggctg
    13621 ttccagttcc agtaattgca actagtttgt tcatgaggaa tctttcacag gaagaagata
    13681 gtttttcagc aaaagtagtt tcagcactaa gaaatgggtt tggcggacat gaaatagttg
    13741 aaaagggtga gtaaaatgcc agattatcat atagcaatcg ttaattccag cagtttcgga
    13801 agagtctttc cacaccactt ggaaagatta aagtcagttg gagacgttca tttctttact
    13861 gttgatcaag agattccagg aaaagaacta gcggagttac ttaaaggatt caatatcatt
    13921 attgcaagtg ttacgccatt ttttcgacga gatttttttg aaaacaaaga tgaactgcta
    13981 ttgatttccc gacatggtat tggttacaac aatattgatt taaaagcagc aaaagaacac
    14041 cagacaatcg tatctatcat acctgcttta gttgaacgaa atgctgtagc agaaaataat
    14101 ataactaatt tgttgtccat gcttcgacgc acgacacaag cacaagcaag agtcaaggac
    14161 gatcgatggg aagatcgggc ggagttcgtc ggtaggacgc tgtttaacaa aacagttggt
    14221 gtgattggtg caggaaatac cggaagcgga gtaatcgaaa ttttgcgtaa cggttttcgc
    14281 tgtgatgttc tagcttatga tccttacaaa tcagatttat acatcgaaag ctttggtgca
    14341 aaaaaagtag aacttcaaga attactggaa caatcagata tcatttgttt atgtgcgaat
    14401 ttgacagaag agaactatca catgatttct gataaagaag tcgatcagat gaaagaaaat
    14461 gtttatctat ctaatagtgc aagaggcgct ctagttgatg aagaagcgat gattaatggt
    14521 ctgaaaagcg gaaagatcgc aggatatgcc acagatgttt tagaagaaga accgggacgt
    14581 caagatcatc cgtatttagc gtttgaacaa gtagtgatta cgccacatat atcggcctac
    14641 acaagggaat gtttagaagc aatgggtgaa aaatgtgtca gagatgtcga acagatcagt
    14701 cagcaccttc ttcctgaacg ttctgtccag acaaagagca actatataaa aggatgatga
    14761 agaaaatggt gaacggttta aaagtgaaaa caggccctca attttattta tatgaagaag
    14821 gtggtatttc aaaagtttct gatctcttga aatcatacgg cgcgaaaaga gtactggtca
    14881 cccacggaac ggtatcatgg gaaaaggcgc tacctaaatt agtatttttg aatgacgaaa
    14941 cgattcaatt tttttatcat cgttattctg gagaatgtag ttatgctgaa gccagacgaa
    15001 tagctacaat catcaaaaaa atgaaatcga tttcgtagtt ggcgttggcg gaggaaaact
    15061 gaccgatcta gtgggttata gtgccaatct tgcaagaatc gatttcggag tcatccctac
    15121 tttagctagt aattgtgctc catggacacc tcttgctgtc atgtataagg agaatggaca
    15181 agcagaaggg aaaacagaac acttttacag acaagctgct ttttttataa cagatcccca
    15241 attagtgatt gattcccctg ttccatattt cattgccggg ttagcagata cactagcaaa
    15301 atggtatgaa tctgagctca tcttagaaca agattttttg caagatgaat catttttgaa
    15361 gctagcacaa gatactgcga agatctgcaa agaagagatt ttgaatcatt ctgctaaagc
    15421 aatccaagat atggcacaaa gaaaactgac atcggagttc aaacagcttt cagagattat
    15481 ctttgctgtt gctggtttag ttggaggatt aggagataaa tacgcaagaa atgcggcagc
    15541 acatgctatg catgacgcta tcagtaaata tattccggaa agccatcgtt ttctacatgg
    15601 agaaaaagta gcgtatggga ttttctatca actggcacta gaaggtcgct gggaaaaaat
    15661 agacgagtta tatcctttgt atcgggaatt agaactgccg gtttctctag aacaaatggg
    15721 attatttccg aaagataaac agataataga taaaatggct gaatttattg attcgaaaga
    15781 gaaagtgcat ttgattccta tagaggtaac acagaaatca ctgatacagt caatgatgga
    15841 actggaaggt tatatcagaa agatctgaag gaggaggaca catggaaaca ttaagtgtag
    15901 tacaaagttt gttgattgct ttatgggtag ccgcggttat gtcccgctgg ttaggtggag
    15961 gagccacgtt gaccttacgt ttttctcctt tgatgactgg attagtcgtc gggttgatca
    16021 tgggggaagt tgcacaagcg atgattgtta cagccgcttt gcaaatgatt tatatggggg
    16081 ttttttctcc aggaggatca atgccagctg aaccatctat tgcagcagct atcgcagtac
    16141 cagtcgcatt attaggggat ctgtcgccag aagcagcgat tgccgttgcg gtgccagtcg
    16201 gattgctcgg aagttacttg tatcagtttc gttttttcat caatactttt ttaggtaaat
    16261 atacagacaa agctgtagaa gatttgaata caaaagctat ggcccgatca attattcttt
    16321 atccaacggt tgcctcattt ttattattcg ttccgcttgt tttcattgct ttatatttag
    16381 gggcgccatt gatcgctgat gtaatcaaag ctttagaggg aacagtcgtt atccacattc
    16441 ttgaagtagt aggtggaggg ttagcggcaa tcggtatcgc gacaactgta tacgttatcg
    16501 gtcgaaaaga ttatctggtc tttttcttcc ttgcttattt tatgagtgtt gttttgaaat
    16561 ctttagatat tacgatggtt acttatgcca tttttgggat tattatcgct ttgatctttg
    16621 tccagtcgca aaaagggaaa caagtacagc ctgccacaag caataacaat tcagcatctt
    16681 ttgatgacga agatgactat gatgacggat tttaatttag gaggaatggg aaatggctga
    16741 aataatggat aatcaaacag gtgctcctga agaaatcacg aaaaaagatg taacaaaagc
    16801 ctatctgaga tggcattttg caaacgaaat tcctcactct tttgaacgtt acttggcgcc
    16861 atcactacta tacggaatga tgccaatctt gaaaaaactt tataaagatg aagactcttt
    16921 aaaagcagca tataaacggc agttactgtt cttcaacact caattaagct ggggtggcgg
    16981 tgtgattact ggcctaatgt cttctatgga acagcaaaga gcagaagaag agtatgaaga
    17041 aaaagagatc ttgatgcagg atgacttgat gtataacacg aaagcgggat tgatgggggc
    17101 actagcagga atcggagatg caatcgattc aggaaccgtc caatatattt ttatcgcaat
    17161 cgcggtacct tgggcacaac agggaagtcc tttaggcgca ctttttcctt ttatctgctt
    17221 tgggttgtat caggttttac taggggtatt tttcgcaaga caagctttca ctatgggccg
    17281 taatgctaca ggcctaatgc aaaacactgg tgttcagaca gcgattgaaa tgctatcagt
    17341 tttagggttg tttatgatgg gggtgcttgc gggaaactat gtaaaagtag aatcttcatt
    17401 gaaatttgcg atatccggac gtgagtttgt tatccaggaa attcttgatc aaatcgtgcc
    17461 agggatatta cctttagctg tcgtaatggg tgtgtacttg ttctatacga aaaaaggatt
    17521 gaaagtcaca caagcattat tatggctgac tggaatatta attgtgctag caggaatcgg
    17581 tattttataa aaagaaggga acgtgaataa gatgggtgta gtcaatttag cgcgtgtgga
    17641 tgagcgtttg atccatgggc aagtaatggt aacgttatct caaaaaagtg gagtgaattc
    17701 catttttgtt gtagacgaag ttgtagcaaa agataaattt atgagagatc tttacaaaaa
    17761 tgcaggaagc cgtacaggac aaaaaacaat tgtcattaca ccagagaaag ctaaattcta
    17821 ttgggatgaa tatcaattta aagaatacaa ttgtattttg atcgcaaaaa cagtccaaac
    17881 gatttacgac ttagtaaaac acggaatccc catggaagaa ttgaatattg gagggattgc
    17941 tcaaaaaaat cctgatacag atcttttagt gacgaaatct gtttatttaa ataaatcaga
    18001 tgctgaaaaa ttgaaagaaa tgaatgaaca atatggcgta aagaatattt attttcaagc
    18061 cactccatct gcacctaaaa caggattaaa agatgtattg aacaagtttg atttgtcata
    18121 aacaaatgct tagaaaaaag ataaagaggg cgaaaaaatg aagaagaacg agctgtttcg
    18181 tgattgggaa tttcgttatc gctatattta ccgtaagcgc cgtacaaaaa agagtaaaca
    18241 gcgttttctt tcagcattag tatcagatat ttacagtatg agaacagatg tgacagtcat
    18301 tgcttacgat acgctagcct accgatcaaa aaatatttat gttggagata tcgaaaaagc
    18361 agagaaagtt atctgtactt attacgatac acccgttcat gcattaggaa gttatttcat
    18421 gtttgattgg aaagaccaaa ggaaaaaaac aatctactct atattattgt ccttcatttt
    18481 gttgttcagt ttaggttggt ggggaatgat gatatataac aaaaatcctc atcacgtatt
    18541 cgatctgcta tccgtataaa caggtatcac tgtactagca tttgggagct actttttctt
    18601 attgggaaaa gcagctcgcg gatggcctag cagacaaact tttattcgaa atacttcatc
    18661 catattaacg atgctagaaa tgatccgtac gattgatgat ccgagtgtag cgtatgcgtt
    18721 tgttgatgaa ggctgttatg gagaaaaggg cttagacagc gtgagatcgg gtatgaaaaa
    18781 agaggctatt cttttttatc tagactctgt tggggcagat acaccgctac agttttcggg
    18841 aaattatttt tcaaataaag aacagtggct gaaacaagtt gataagttga aagaaaaaaa
    18901 cgtcaattat atttttagtg caagaaaaaa gcaagcgcag ttcttctatt tgaccaaaac
    18961 agatttacga gggaaaacat ttaattggca aaatgccaat cagattattg ctctttttcg
    19021 ataagtaaaa agtgcaaaac aaggagtgag aagactaatg ttggaaatca tgatcgttac
    19081 acatggtaag ttgagcgaag gactgaaaga tgcggcggaa gtaattgtcg ggacatcgag
    19141 ccatattgaa acaatgtgct tagagtctgg ggaggacatc caaacattcg gagagaaggt
    19201 caaagaaaaa ttaactatga tcaacacagc agaacccatg ctagtatttg ttgatctaat
    19261 tagcgcaagt ccgtacaatc aagcgctctt ggcagtcaat gaactgactg aagaaaagaa
    19321 agcagccact tatgtcatag ggggcgtgaa tctgccaatg ttgttagaag caatcaatca
    19381 tcagatgctt cacacaccga tagaagaagc ggtggaacaa attgtcaaac aaggaaaaga
    19441 taatttaact gtttggcatg cttcagcgga agaaaaaaaa ttagatgagg acgattttta
    19501 gaaggaaggc tgaaaagatg aagtggggat ttagatggta tggtgaaaaa ggggattcga
    19561 tcccattaaa atatgttcgc caaataccgg gaataaatgg gataattgga actttgctga
    19621 ctaaaatgcc tggagatgtt tgggagattt cagaaattga ggagttgaaa gcatctgttg
    19681 agagccaagg actggaactg cttggaattg aaagtgtagc gatccacgat gcaatcaagg
    19741 cagggacaga tgaacgagat tactatattg ataactatat acgaacaatc aaaaacttgt
    19801 ctcgatgtgg ggttcgattg atttgttata gcttcaaacc aatttttgga tgggcgaaaa
    19861 ctgatttgtt taatgaaaac aacgatggaa gctattctct tttatacgat cagaaaatcg
    19921 tcgatcagat ggagccagga gaaatgtatc aattgataca cagccagtca aagggattca
    19981 aattgccggg ttgggaagaa gaacggttga aaaaattcgc tcagctggaa aaactgtatg
    20041 aaggaatgac cgaagaagaa ttatttgaaa atctgactta ttttttagaa aaagtgattc
    20101 cagtgtgcga aaaagcagat gtaaaaatgg ctatccatcc agatgatccg ccatgggaac
    20161 tttttggttt tcctagagtg acgaaaaatc ttggcgactt gaagaaaatt ctaaatgcag
    20221 tagattctcc atataatgga ctaacgctgt gcacaggatc tttaggggca aatccggaaa
    20281 acgatttagt tgaaatcatc catgaggtag gttcgcgcat ccattttgtc cacttcagaa
    20341 acgtggagtt tttaggagaa agaaagttca aagaaacggc acatccctct acagaaggct
    20401 cccttgatat gtatcatatt atgaaagcgt tggtgcaaga agggttcgac ggtcctatac
    20461 gccctgatca tggtagaacc gtgtggggtg aagtggctat gcctggatat ggcctgtatg
    20521 atcgtgcaat gggactcact tacatgcaag gtctatatga ggcgataagt aaggaaaaac
    20581 cactgggaga gcattatgaa taaaatcaag ctcttcatct ttgatatgga tggcctttta
    20641 ttagaaactg gacgtttagc ttatcgtgct tatgtaaaat cagcacaaaa gtatgattat
    20701 gaaatgcgta aggaagtcta ttatttacta actggccaaa cagaaatggc gataagagaa
    20761 caaatgggca ttttgtacgg agaagatgtt ccatatatca aatggcgaga agcaatcaat
    20821 caatataaag aaaaaatcgt gaaagaagat aagagagtat atacgaaaaa aggagcagaa
    20881 gaaattttat cttttgcaaa agaaagagga atccatacaa tcgtggcttc gtctaataca
    20941 cgggtaaaag tagaaatgta tttaaaaatg gaaaatctat atgccttgtt tgatcaaatc
    21001 atttcagggg atgatgtaaa aaaaggaaag ccggaaccag agatcttttt gaaagcttgt
    21061 tcaaaaatga atattcctcc aagtgaagca cttgtatttg aagactcgat tgcagggatc
    21121 gaagcagcaa ggcgagcggg cattttgtct tttctcatca aagatcattt gatagggttg
    21181 cccgatcata atgggaagca taagttgaaa gtagatgtaa gtttatatga atccaataaa
    21241 gcagactatg aattttatga cctgcatcaa gcaaaacgat ttttagaaag taaagggttg
    21301 tatctctaat aaagaggact attttctagt cctctttatt tattgtcctg cacgactgag
    21361 ggaacagatg aaaaagaagt aattttttat tttgaagtat ctaccttatt tgtagtgaac
    21421 aatttagaac aaacacaaaa cgcatctact gttatctacc agtagatgcg tttaaagaga
    21481 agaacgagaa tcgtccatat gagggtttcc actccttatg cagatttttt taattcttgc
    21541 actgcttgtt cgatcgtaac acctgtcgct acatgattgg tattttcttt gtcaaaaacg
    21601 acaaatagat tcaaatctgt atccaaggtt actcgatatt gtatatcttt gttatagaat
    21661 gtcatagcca ttctcctttc tttaaatact tgctgaaagg caacgaacgc gttgataacg
    21721 aaattccatt tataagtata ccaccaattg aaaggttttc atagttaaga aattttaaag
    21781 tcaaaagttg cagataaaag caaataaaca agaaaacaaa aagtgaatca atcgcggtta
    21841 ttcaactttt tcttcgattc gaagacaata gaagacaatg aattttatat ttcattgcat
    21901 tatccttgtg ttattagaaa aagcatgata tattaaatga gtaatctatt ttgaaacgta
    21961 atttgagcga tatatctaca tagataaaac tccttttgcc tatcaaatat ttattgcaac
    22021 gaaaaaacgt acattgatac gtatcaggag gaatttattt atgaataacg gtacagtaaa
    22081 atggttcaac gcagacaaag gttttggctt tatcactggt gaagatggaa atgatgtatt
    22141 tgcacatttt tcagctatcc aaggtgacgg cttcaaaact ttagaagaag gccaagcagt
    22201 gtcttacgat gttgaagatg gtcaacgtgg ccctcaagca acaaacattg ttaaagctta
    22261 ataaaaattg acttttaacc gtcttttcga aagaaaagac ggtt
//
LOCUS       DAMYXM010000042        22283 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.043, whole genome shotgun sequence.
ACCESSION   DAMYXM010000042 DAMYXM010000000
VERSION     DAMYXM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.043"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            24..266
                     /gene="yaaA"
                     /locus_tag="PNS21_001778"
     CDS             24..266
                     /gene="yaaA"
                     /locus_tag="PNS21_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDI5159488.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            253..1377
                     /gene="recF"
                     /locus_tag="PNS21_001779"
     CDS             253..1377
                     /gene="recF"
                     /locus_tag="PNS21_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDI5159489.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1374..3320
                     /gene="gyrB"
                     /locus_tag="PNS21_001780"
     CDS             1374..3320
                     /gene="gyrB"
                     /locus_tag="PNS21_001780"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDI5159490.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3340..5811
                     /gene="gyrA"
                     /locus_tag="PNS21_001781"
     CDS             3340..5811
                     /gene="gyrA"
                     /locus_tag="PNS21_001781"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDI5159491.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6047..6346
                     /locus_tag="PNS21_001782"
     CDS             6047..6346
                     /locus_tag="PNS21_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDI5159492.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6396..6908
                     /gene="ssb"
                     /locus_tag="PNS21_001783"
     CDS             6396..6908
                     /gene="ssb"
                     /locus_tag="PNS21_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI5159493.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6934..7170
                     /locus_tag="PNS21_001784"
     CDS             6934..7170
                     /locus_tag="PNS21_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDI5159494.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7313..9286
                     /locus_tag="PNS21_001785"
     CDS             7313..9286
                     /locus_tag="PNS21_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDI5159495.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9292..9744
                     /gene="rplI"
                     /locus_tag="PNS21_001786"
     CDS             9292..9744
                     /gene="rplI"
                     /locus_tag="PNS21_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDI5159496.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10018..11385
                     /gene="dnaB"
                     /locus_tag="PNS21_001787"
     CDS             10018..11385
                     /gene="dnaB"
                     /locus_tag="PNS21_001787"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDI5159497.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12165..13457
                     /locus_tag="PNS21_001788"
     CDS             12165..13457
                     /locus_tag="PNS21_001788"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDI5159498.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            14244..15107
                     /locus_tag="PNS21_001789"
     CDS             14244..15107
                     /locus_tag="PNS21_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDI5159499.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(15236..16858)
                     /locus_tag="PNS21_001790"
     CDS             complement(15236..16858)
                     /locus_tag="PNS21_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI5159500.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            17107..17628
                     /locus_tag="PNS21_001791"
     CDS             17107..17628
                     /locus_tag="PNS21_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDI5159501.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(17814..19544)
                     /gene="spxB"
                     /locus_tag="PNS21_001792"
     CDS             complement(17814..19544)
                     /gene="spxB"
                     /locus_tag="PNS21_001792"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDI5159502.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            19709..20527
                     /locus_tag="PNS21_001793"
     CDS             19709..20527
                     /locus_tag="PNS21_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDI5159503.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(20630..20827)
                     /locus_tag="PNS21_001794"
     CDS             complement(20630..20827)
                     /locus_tag="PNS21_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159504.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            20996..21574
                     /locus_tag="PNS21_001795"
     CDS             20996..21574
                     /locus_tag="PNS21_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDI5159505.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            21669..21806
                     /locus_tag="PNS21_001796"
     CDS             21669..21806
                     /locus_tag="PNS21_001796"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159506.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         7159 a         4022 c         4699 g         6403 t
ORIGIN      
        1 ataatgaaaa tgagggatgg gcattgaaac aacaagtcat tctaaatacg gaattcatga
       61 cgttgggcca aatgttgaaa gaagtcacag ttatcggtag tggcggacaa gcaaaatggt
      121 atttggcaga aaacactgtg ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac
      181 tttatcccgg catgatggtt gaagttccag aagtaggaac tttttttatg gtaaaaaaag
      241 gaaacgacga taatgaggct gaataagctg tatttaaaaa attatcgaaa ttacgaagag
      301 ctgaacatcg aattttccaa gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa
      361 acgaatcttt tggaaagtat ctacgtatta gctatgacgc gaagtcatcg gacaagtagt
      421 gagcaggaat tgattggctg gactgacgat caggcgatga ttcaaggaga gatcacaaaa
      481 ggttcatcca ctcttccatt agaaatctta ttatctaaaa aaggccgaaa aaccaagatc
      541 aaccatatcg aacagaaaaa gttaagtact tacgtgggac aaatgaatgt cattttattt
      601 gcaccagaag atctttcttt agtaaaaggc agcccgcaga tcaggcggaa gtttttagat
      661 atggaattag gccagatcaa tccaatctat ctttatgatc ttgtccaata ccaagcaatt
      721 ttgaaacaaa gaaaccagta cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt
      781 tatttggata ttttgtccga acaactagca gcgtttggca gcaaagtatt gaaagcaaga
      841 gcacagttcg tcaagcgatt ggagttttgg gctaattctt tgcatcagca aatcacccat
      901 caaaaagagc agctggaaat cgagtatctc accgcagtcg attctttaga aacacatacg
      961 caagagcaaa tccaagaaca atttctcgca cttttgaacc aaaataaaaa gaaagacctt
     1021 tttcggggaa caacgacagt tggtcctcat cgtgacgatc tttccttttt tattaatcag
     1081 aaaaatgtgc agacttatgg atcgcaagga cagcaaagaa caactgcact aagtgtcaaa
     1141 ttagcagaaa tcgatttgat taaagaagag acaggggaat atccaatcct tttgttagat
     1201 gatgtgatga gcgagctgga tgataatcgt caactccatc tgctagaaac aatcgaagga
     1261 aaagttcaaa cgttcttaac aacaacgaca cttgaccatg taaaaaataa aatgactgtt
     1321 gaacctgaga ttttttatgt tcaacaaggg aaactaaaag gagaagaaac tgaatgacag
     1381 aagaaagaag tttagtagaa cgcgctaaag agtatgatgc cagtcagatt caagtacttg
     1441 aaggacttga agccgttcgt aaacgacctg gcatgtatat cggttcgact agttctgagg
     1501 gtctgcatca ccttgtttgg gaaattgtcg ataattcgat cgatgaagta ttagcaggtt
     1561 ttgcaacgaa aatccatgtg attattgaaa aagacaacag tatcaccgtt attgacgacg
     1621 gtcgggggat acctgtagat atccaggcaa aaactggacg tcctgcagtt gaaacggtct
     1681 ttactgtttt acatgctgga ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac
     1741 ttcacggagt aggatcttct gttgtaaacg cgctttctac acagcttgat gtcaaagtat
     1801 acaaagaagg aaatgtgtac tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa
     1861 agattatcga acaaacggat cgccatggaa caacggttca ctttacgcca gaccctgaaa
     1921 tttttacaga gacaactgag tttgattttt caaaattggc tacccggatc cgtgaacttg
     1981 ctttcttgaa ccgcgggatg cgtatatcga ttgaagacaa acgagaagaa gaaccagtca
     2041 tcaatgaata ccattatgac ggcgggatca aaagttatgt cgaatacttg aatgcaaaca
     2101 aaaccgtgat ttttccagaa cctgtatatt tggaaggtga acagcaagat atcgcagttg
     2161 aagtctctat gcaatataca gacggctacc attccaatat catgagtttt gccaacaata
     2221 tccatacgta tgaaggtgga acacatgaat caggattcaa gactgcttta actcgtgtga
     2281 taaatgatta tgcccgaaaa caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg
     2341 aagacgtgcg agaaggattg acagctgtta tttctatcaa gcatcctgac cctcaattcg
     2401 aaggacaaac aaaaacaaaa ttgggcaatt ccgaagtacg gacagtcact gatcggttat
     2461 ttagcgaaca tttcatgaaa ttcttgctgg aaaacccaag tgtgggccgg caaattgttg
     2521 aaaaaggatt actagcttcc agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga
     2581 gacgtaaagg tgctttagag attagtaatc tgccaggaaa attagcggac tgctctagca
     2641 acgatcctga aaaatgcgaa ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta
     2701 agcaaggtcg taatcgagag tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg
     2761 ttgaaaaagc aagcatggac aagatcttgg caaatgaaga gatccggtct ttatttactg
     2821 ctatgggtac agggtttgga gaagattttg atgtatcgaa agctcgctac cacaaattag
     2881 ttatcatgac tgatgccgat gtcgacggcg cgcatatccg tacattgctg ctgactcttt
     2941 tttatcgtta catgcgtcct attgttgaag ctggatacgt ctatattgct cagccacctt
     3001 tatacggggt aaaacaaggg aagaatatta cgtatgtaca accaggaaaa aatgcagaag
     3061 aagagttgaa acaagtcgtt gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt
     3121 ataaaggact tggagaaatg gatgatcatc aactatggga aacaactatg gacccaagca
     3181 atcggatgat ggcccgcgtc agtgtggacg atgctattgc agcagatcag atatttgaaa
     3241 tgttgatggg ggatcgagta gaaccccgtc gtgcattcat cgaggaaaat gcacactatg
     3301 tgaagaactt agatatttaa tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa
     3361 aacatccatg atgtcaatct aaccagcgaa atgaaagact ccttcatcga ttatgcgatg
     3421 agtgttattg tcgcacgtgc acttcctgat gtcagagatg gtctgaaacc cgttcatcgc
     3481 cgtattttat acggcatgaa cgaattaggt gtaacaccgg ataaagccca taaaaaatct
     3541 gccagaatcg tcggagatgt catgggtaaa taccatcctc atggagacat tgcgatctat
     3601 gagtcaatgg tacggatggc acagccattc agctaccgtt atatgctagt ggatggtcat
     3661 ggaaactttg gttctgttga cggtgacggt gccgccgcta tgcgttatac cgaagcacgg
     3721 atgagcaaaa tcgctacaga aatgctgcgt gatatcaata agaacactgt tgatttccaa
     3781 agcaactatg atgatacaga aaaagaacca gttgtactac ctgctcgctt cccgaatctt
     3841 ttagtgaacg gaacaacggg gatcgccgta ggtatggcca cgaatatccc acctcataac
     3901 ttgtctgagg tagtagcggc tatcgatttg ctgatggata atcctgatgt gacgaccaat
     3961 gaattgatgg aagtattacc tggaccagat tttccaacag gtggtttggt tatggggaaa
     4021 tcaggaatcc gaagagcgta tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt
     4081 gagattactg agatgccaaa tggtaaagaa cggatcatcg tgacagaatt gccttatatg
     4141 gtcaataaag caaaattgat cgaacggatt tctgaactgc acagagacaa gcgtatcgaa
     4201 gggatcacag atttgcggga cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc
     4261 cgtcgtgacg ccagcgcatc cgttatcttg aataacttat ataaattgac atccttgcaa
     4321 aattcatttg gattcaatat gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg
     4381 aaacaaatcc ttgaaaacta tatcgaacat caacgcgaag tcatcacacg acggacggtc
     4441 tttgaaaaag aaaaagcaga agcaagagca cacatcttag aaggtttgcg tattgctttg
     4501 gatcatatcg atgaaatcat tgcgattatc cgcgggtcta aatcagatga cgaggcaaaa
     4561 gcaacaatga tcgaacggtt cgatttatcc gatcgccagt ctcaagctat tttagatatg
     4621 cgtttacgcc gattgaccgg tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc
     4681 ttaaaactga ttgaagattt aactgacatt ctggcaagac cagaacgtgt tacagaaatc
     4741 atcaagaatg aattagcaga cttgaatcaa cgatttggtg ataaacgacg cacagaatta
     4801 ctagtcggtg aggtcttaag tctggaagat gaagatttaa tcgaagaaga agaaatcgtt
     4861 attacattga caaacaatgg ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa
     4921 cgaaggggtg gacgtggtgt ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat
     4981 ctggtttctt gttctaccca tgatacattg ttattcttta cgaataacgg aaaagtgtat
     5041 cgggcaaaag gatacgaaat tccagaatac gggcgtacag ccaaaggaat cccaatcatc
     5101 aatttactag gtatcgattc gagtgaaagc atccaggcaa tcattgcggt gacgggagaa
     5161 gcagaagaag gccactatct cttctttacg acgcgtcaag gtacagtaaa acgtacttct
     5221 gtcactgctt ttgctaacat ccgaagtaat ggtctgatcg caattggttt gaaagaagac
     5281 gatgagctcg tcaatgtgct actgacagat ggtcaatcta atatcattat tggaacacat
     5341 aacggctact ctgtcacttt caaagaagaa gcagtccgcg atatgggacg tacagcttcc
     5401 ggggtcagag ggatacgcct gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag
     5461 gaaaatcaag aagtattagt gatcacagaa aaaggttacg gaaaacggac aaaagtatcg
     5521 gaatatccag tgaaaggccg cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa
     5581 aatggtccac tagctggatt gaccactgtt tctggagatg aagacatcat gctgattaca
     5641 gataaaggtg tcattatccg tttcaacgta tcgactgttt ctcaaactgg acgttcaaca
     5701 ttaggcgtac gtttgatgaa gatggaagct gatacgaaag ttgtcacgat ggcagcagtc
     5761 gaaccagaag ctgcagaaca aacagttgaa gaacaaggat cagaagaata accatcaaga
     5821 ctaagtgtgt aagctagcaa gaataaccga ttaaaaaatc tggttattcc tgctggcttt
     5881 tttgattagt tgaatttctc ttaaaatatt cgaaactgtt cattctgtat tgcttttgac
     5941 caaaatagag tgtataatta gagcttgtga gtagtctaat taattagata actctccttg
     6001 ctcttaggaa cctaagagcc gaaagtccac agggaggtga atatcaatgg aaaacacgaa
     6061 atatgaaatc atgtacatta ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat
     6121 cgaacgtttc gatgcaatct tgaaagataa cggagcagaa gttatcgaat caaaagattg
     6181 ggaaaaacgc cgtttcgctt atgaaatgaa cggttaccgt gaaggtatct atcacatcgt
     6241 taaagtatct tctccatcat cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat
     6301 caacgacgat atcattcgtc acatgattgt taaagaagaa gcttaattgt tgtttcatgt
     6361 gaaacaatag aaaacgatga gaggagatga ctattttgat taataatgtt gtattagttg
     6421 gacgtctgac gaaagatcca gatttgcgtt acacagcaag tggtacggca gtcgcaacat
     6481 ttactttggc tgtgaatcgt aattttacaa accaaagcgg caatcgcgag gctgatttta
     6541 tcaactgtgt gatttggcgc aaatcagcag aaactttagc aaactatgct cgtaaaggaa
     6601 cacttttagg tgttactgga cgtatccaaa ctcgttctta tgataatcag caaggacaac
     6661 gtgtatacgt gacagaagta gtagctgaca acttccaatt gctggaaagt cgttctgcat
     6721 ctgaaaatcg ccaacaaagc ggcggattcc aaagttctgg tcaatcagct ggcggatttg
     6781 gcggaaacaa taactctaat caaacatctc aatcatccaa cggtatgcct gatttcgacc
     6841 gtgatacttc tgacccattc ggctcaagtt caacgatcga catttcagac gatgatttac
     6901 cattctaaac gaatttgata ggagggaaat agaatggcac aacaaagaag aggcggacgt
     6961 aaacgtcgta aagtagacta cattgctgct aaccatattg aatatatcga ttataaagat
     7021 atcgaattac taaaaagatt tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact
     7081 ggtacaggcg ctaaaaacca acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg
     7141 ggcttgctac ctttcgttag cgaagactaa taaatcctag gaattgaatg actgatcctt
     7201 ttgatcagct attcaattcc tttttttctg taaaaataac ctctattcgt ttagtcgctc
     7261 gaatgacgaa agttatggta aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa
     7321 taaaaacaat catttatccc cgaaacctct cttccttatc ctgtttatat taatcgaagt
     7381 attggtagtc ttaattttac catttcgttt tgttagcgct tcggtcattc tattgatgaa
     7441 tttatggggt gttcgctatt ggttgaaaat gaaacgttgg cttattttaa ataaacgtga
     7501 aaaaatccag caggcttcga ctgtagctga aaccaatcta gcttatgtct cagaagtcat
     7561 gcctgtagga gtgattgctt atcagccatc aagccaaaaa atcgaatggc tcaatccatt
     7621 agcattgaaa atcaaagatc aaagtggatt aacagaggaa gaattgctag atactttttt
     7681 tactgcaaaa gaaaaaaaga aaaaccaagt ctatctagca gataaaacat ttcagtttga
     7741 tatggataca gtaaagggtg ttgtttattt taatgatatt acccaaaacc agttgttaaa
     7801 actccatcag acgaacagtc agccggtgat tggtatttta tctattgata actatgcaga
     7861 tgccattgat aaattagatg acaaagagat ttcttatttg aactctttcg tcactacact
     7921 agtttctgac tggatgaatg aataccatgt ctttttcaaa agaatcaacg cagaacggtt
     7981 tttcttcgta gcacagatga tcgatctgaa aaagatgatg gataaaaaat ttgatttgct
     8041 ggatcgcttt cgagcagaag ctgaaaaaca gagctcgcct atcaccctta gtatggggat
     8101 tgcttatggc tcaagaaccc tcgatgaaat cggtggagaa gctcagaata atttagatgt
     8161 tgctttagtc cgtggaggcg accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc
     8221 tgtttattat ggggggaaat ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc
     8281 gatgagtacc gctttgcgtc ggatttttga tgaaacaggc gatgtgttta tcatggggca
     8341 caagtttccg gatatggatg ctttaggctc tgcttttggt gtcagttgtc tggcacgctt
     8401 caaccagaaa aaagcttaca tcgttatcaa tgagaacgag atcattccag atgtagaacg
     8461 gtgtcttgaa gaaatccata agcatcctga attagaaatg caactaatct ctccagaacg
     8521 tgccatagag ctgaaaaaag aagaggacct cctcgtgatg gtggattacc ataaaccttc
     8581 tctatcgatc tctcagggcc tatatgagca atttgataag attgtgatca ttgatcacca
     8641 tcgtcgtggg gaagagtttc caagtaaacc tctgctgact tatattgaat cttcagcttc
     8701 ttcagcttca gagctagttt ctgagctgat ccaataccaa agcagcaaaa caaatcgctt
     8761 agatcgtttt gaggccacat tattgcttgc agggatggtt gtagatacaa atagtttcaa
     8821 tgtccgtaca actgcacgaa cttttgacgt tgccagctat ttaaggtctt gtggagcaga
     8881 cgcatcctta gtacaatatt tattgagctc ggatttagcg acatacttgg agatcagcaa
     8941 tttgattgct ggaagcgact atgtgacttc tgatattgtt gtggcggcag ggacagacga
     9001 aaaagagtat aatagtgtta tagcagcaaa aacagcagat acattgcttt cgatgatcaa
     9061 tatcaatgca gctttcgtca ttacaaagcg gacagacggc ttgattggga tcagtgccag
     9121 aagtaccggc acgatcaacg tccaaatcat catggaatcc ttaggtggcg gaggacactt
     9181 cacaaacgca gctactcaac tggaaaatac gacagttgag gaaacaaaac agaagctatt
     9241 tgctgcaatc aaagaaaata tgaatcaaat atatgataag gagtaatcca aatgaaagta
     9301 atttttttac aagatgtaaa aggaaaaggt aaaaaagggg acatcaaaga agttccagca
     9361 ggttatgcac aaaattatct aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt
     9421 atcagtcagt tagcaggaca aaagaaagca caagaaaaac acgaagctga aatcttggaa
     9481 gaagctaaaa agttaaaaga attcttggaa aaagaagata ctgtagtcga attgaaggcg
     9541 aaagcagggg aagacagccg tttgtttggt tcgatccctt caaaacaaat tgctgaaggg
     9601 ttaaacaaac aatacaaaat caaattagat aaacgaaaaa tcgaattaga aaatccgatc
     9661 cgttcactag gctacacaaa cgtacctgtt aagcttcatc atgaggtgac ggctacgatc
     9721 aaagttcatg tcgtacaaga ataacaaatc ttgatagcga tgttcagctg gtaagtataa
     9781 ccagaaggaa aaaagacaaa ccgaaattta tctgagaaaa cagctgcagc aatgaaagca
     9841 aaagctgcaa atcgatttca gattgatttc ggtttctttt ttgggaacag caaaatagca
     9901 taaagaaaac gagtataata aaagagaact atttttttat tacactttcg gtacgaatga
     9961 tgacttttct ctcattttct tgtaaaatga agcaattgaa gacaaggagc gatacgtatg
    10021 aatgaagtgt ggcaggatcg aatcccaccg caaaatattg aagctgaaca agcggtttta
    10081 ggctctgttt ttcttgacgc agaaacgatc attgatgcca tggagtacat agagccaaaa
    10141 gacttttatc gtcgtggcca tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt
    10201 aatgaagcga ttgacgtggt tactgtcaaa gaccggctgg aacaagccaa tttgttagaa
    10261 gatgccggtg gtttaagtta tctctcagac ttggcattag cagttccgac agcagcaaat
    10321 atcgtctact atgcgaaaat cgttgaagaa aaatcattgc tgcgtacatt gatccagaca
    10381 gcaacaggaa tcgttacgaa aggattcgag caaggagaag aagttcaaac aattttagat
    10441 gatgccgaac gcagtatctt ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct
    10501 attgctgaag tactgaactc cacgattgaa aatatcgatc gactagctca aaacaatgaa
    10561 gaaatcacag gtctaccaac tggctatcaa ggtctggata aaatgacagc aggtttccag
    10621 cccgaagaac taatcattct tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg
    10681 aatattgctc aaaatgtagg aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa
    10741 atgggggcag aatctcttgt aaatcggatg ctttgtgcag aaggttcgat tgaggccagc
    10801 catttacgta caggtcagct ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg
    10861 agtctttctc gagcaaatat ttttatcgat gatacaccag ggatcaaaat ctctgaaatc
    10921 cgtgcgaaat gtcggaaact agcgcaagaa aaaggcaatc taggcttgat attgatcgat
    10981 tatttgcagt tgattgaagg taacggtaaa gagaaccgtc aacaagaagt atcagatatc
    11041 tctcgccaac tgaaaaaact ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag
    11101 ttatctcgtg gagtagaaca gcgacaagac aaacgaccag tgctaagtga tattcgtgaa
    11161 tccggatcaa ttgaacaaga tgcggacatc gtagccttct tgtatcgaga tgattattat
    11221 gaaagagaag gcggagatga cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa
    11281 gtcatcatcg agaagaaccg aagcggtgca cgaggaacag ttgaactact gtttatcaaa
    11341 gaatacaata agttttcgtc gctttcgcca agagaagaat tttaaagaag aggttgtgag
    11401 ccttgagcaa tcccgagagc tctcgtaaga agatgcaagg agtaaccgca ggaacgacta
    11461 gagtacttaa tttaaacaga tctccaaagg gattgtgaca aaagtcttgt cacagtccct
    11521 ttttccgaat aaacggtgtt caatcagctg acccttggta ttagctcagc aaacgtcaaa
    11581 atatgaggaa ctattttgac gtttgtttct ctaatactca ggtcaaaacg ctgatcttac
    11641 gacctctttt ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatca
    11701 tcaaacatga gaagcagttt tttgaaattg ctccttgtga ctcagagtta aacggcaggc
    11761 tcacaacctc tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca
    11821 aacgaaaaat atgagaagct atttttgttt ggctgttctt attacttgat gcaggacagc
    11881 tttttcacaa cctctgattc acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa
    11941 atttcacaaa aatttgagaa gcaattttcg tacattcctt cttatttctt gaagctgacc
    12001 acttctgtta caacctcttt tttaataacc attctaaaaa gataaagttc gttttttaag
    12061 cgtttttata aaataaaaat aaaaatcatt cgattttatt tgaaatatgt atctattatt
    12121 gatacaatgg ttcaggatta aataatatag aacgaggtgt tcacatgtca tctgtagtag
    12181 tagtaggtac gcaatggggc gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa
    12241 atgctgaagt gattgcacgg taccaaggtg gcgacaatgc agggcacaca attaagttta
    12301 acggtgtgac ttataaactg catttgattc cttctgggat tttttataaa gacaagatca
    12361 gtgtgatcgg aaacggcgtt gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt
    12421 tacatgaaaa aggtgtagaa acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct
    12481 tgccttatca tatcaaattg gaccaattac aagaagatgc taagggcgaa aataagattg
    12541 gtacaacgat taaaggaatc ggacctgcct atatggataa agcagctcgt gtaggtatcc
    12601 gtattgcaga cttattagat aaagaaattt ttgaagaacg tcttcgtttg aatctagatg
    12661 acaaaaaccg tcaatttgta aaaatgtttg attcagaacc acttgatttt gacgatattt
    12721 ttgaagaata ttatgaatac ggacaacaaa tcaaacaata cgtcacagat acttccgtta
    12781 tcttgaatga tgctttggat gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa
    12841 tgctggatat cgaccaggga acttatcctt ttgttacttc ttctaaccca gtagccggtg
    12901 gggtaacaat cggaagcggt gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca
    12961 aagcctatac gtcacgtgtc ggtgatggac catttccaac tgaattattt gatgaaacag
    13021 gagaacaaat ccgcgaagta ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg
    13081 taggctggtt tgattctgta gttatgcgcc attctaaacg agtttcaggt attaccaacc
    13141 tatctttaaa ctcgatcgat gtattaagtg gattggaaac agtaaaaatt tgtacggctt
    13201 atgaattaga tggcgaacta atctaccact atccagcaag cttgaaagag ttgaaccgct
    13261 gcaaaccaat ctatgaagaa ttgccaggct ggtcagaaga cattacagga tgcaaaacac
    13321 ttgcagaact gccagaaaac gcacgtaact atgtacgtcg tatctcagag cttgttggtg
    13381 tacgtatctc aacattttct gtcggacctg atagaacaca aacaaacatc ttagaaagcg
    13441 tctgggcgca aatctaagga taaaaacgac gactgaatca ggacgttctt accagagcga
    13501 gtcgaagtgt ttatctatac gataataata acttgaagag gtagtgaaag agacgtttag
    13561 ccttattgcc gataggctgt ctcttgaaca ctgtttattc agaataaggg actgtgacaa
    13621 aaaccgtgtc acagtccctt tttccgaaca aacggtgttc aaaagcttgc atctacggca
    13681 ctaagcccaa tcagatgaaa aatatggagt gctattttta tctgattgtt cttactgctt
    13741 gatgcagagc agctttttca caacctctcc actaaacggt gttctcctgc caactcctcg
    13801 gtcacaagtc acaatattca acaaacatga gaagctgttt tttgaaattg ttccttgtga
    13861 ctcggagcta aacggcaggc tcacaacctc ttggtaaacg gtgttcaatc agctgaccct
    13921 cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttttccgttt ttttctctaa
    13981 tactcaggtc aaaacgctga tttcacaacc tctgattcac ggtgttccag aagtagcttc
    14041 tacggaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa attccttctt
    14101 atttcttgaa gctgaccact tctgtcacaa cctcttattt tgattagaac ttgcgaaaat
    14161 aaaccggtta aagtgtgtct agcgcaagag atttgctata atagacgata agggtaaaat
    14221 gtaacgagaa ggggttgaaa aatgtgtatc aaattgtaac agattcttgt tgtgatctgc
    14281 cataccatgt attagcaaaa gaaaaagttg attttttatc aatgaatctc caattgaatg
    14341 atcaagagtt agtagatgac ctcggaaaga cattcaatta tgaccgctac atgaatcagc
    14401 tgaaaaatgg tgcgatgcct acgacatcac aaatcaatgt tggccgatat ttagaatttt
    14461 tccgttctta tgcggaaaaa cagattcctg ttttatatgt ggctttttcc agtggattaa
    14521 gcggctctta cagtagtgcg cttcaagcgg ttgaattgct gaaggaagaa tatgaagacc
    14581 ctgagatcta tgtttttgat acaaaagctg caagtattgg ggaagggctt ttagtattgg
    14641 aagctgtaag attgaaaaaa gaaggtaaaa gcatcgaaga aaccctagag tggctggaaa
    14701 gtaattattt gaaagtccat tcatgggtaa cagtagacga tctgaaatat ctagaacgag
    14761 gcggtaggat ttccaaagca agtgctactc ttggcagtct attgagtgta aagcctatta
    14821 ttacggtgga taaagaagga aggctgacga atgttgacaa agttcgagga agaaacaagt
    14881 cgatccagac aatcgtcaat gagacggtaa aaacaatcgt tgcaccactt gatcaaatcg
    14941 tttatgtggc ttacgctggg gatctagaga gcgcggagaa ggcaaaacgg cttttggaag
    15001 agcagataaa gatgaaagat gtgaagcttt atcccttagg accaacgatc gccagtcaca
    15061 caggctatgg ctgtatcgcg attttttcga tgggaatcag tcgataaaag cagtaactca
    15121 ttctgtaaaa aagcccagaa attgattgta acaaaaacag cgactcttaa aagatagatg
    15181 agcatcctat ctcttagagg tcgctgtttt tttatttttt aagctaaata ggccattatg
    15241 cccataactc gttcaattgt ttttgaatat ctgcattttc caaaaattca tcatatgtcg
    15301 tatcagctcg atcgatcaca cctttatccg aaacggcaat gatgcggttt gccaatgttt
    15361 ggataaattg atggtcatgt gacgcaaata agattgagcc agtaaatgcc atcaagccgt
    15421 cattcaatgc tgtgattgat tctaagtcta aatggttcgt tggatcatct aatacaagta
    15481 cgttagcttt cgaaagcatc atttttgaaa gcatgcaacg gactttctct ccaccagata
    15541 aaacattcac tggtttcaat acttcttcac cagaaaacaa cattcttcct aagaagcttc
    15601 ttaagaaggt gttatcatct tcttctttac ttgcatattg acgcaaccag tctaaaatcg
    15661 ttagatcagt atcgaattct ttcgttgtat cctttggtaa ataagaatta cttgttgtca
    15721 caccccaacg tactgttcca gtatctggcg taatttcacc catgataact ttgaatagcg
    15781 ttgttgttgt tatatcattt tctgcgacga aagcgacctt gtcttctttg ctcaaattaa
    15841 aagatatatc atctaaaatt ttctttccat caatcgttac acttacattt tctacctgca
    15901 ataagtcgtt tccaatctca cgttcaggtt tgaatccaac gaatggataa cgtctagaag
    15961 aaggttggat atcgtctaaa gtaatttttt caagcatttt tttacgtgaa gttgcttgtt
    16021 ttgattttga tgcattcgca ctaaagcggg caataaattc ttggagttct ttgatttgtt
    16081 cttctttttt ggcatttgcg tttgcttgaa gtttagccgc tagttgacta gattccaacc
    16141 aaaaatcata atttcctaca taaagtttga ttttaccaaa atctagatcc gccatatgtg
    16201 tacatacttt gttcaaaaag tgacggtcat gggatacaac gatcacagta ttttcaaaat
    16261 tgatcaaaaa ttcttccagc caattgatcg attggatatc cagaccgttt gttggttcgt
    16321 ctagcaaaag aacatcaggt ttaccaaata gtgtttgtgc taataacact tttacctttt
    16381 gtccaccagt taattcactc atttgctgat ggtgcaattc ttcagggatg ttcagacctt
    16441 gcagtaaaac agctgcttct ggttcagctt cccatccatt aagctcagca aattctcctt
    16501 ctaattcagc tgctcgaata ccatcttcgt cagtgaaatc ctctttcatg taaatcgcat
    16561 ctttttcttt cagcacttca tacagacgct tgtgtcccat gatgactgtt tcaagtacag
    16621 tatagtcttc atagtcgtaa tggttctgtt tcaatacggc catccgttca tctggtccca
    16681 tagtaacgga acctgtggaa ggctgcagat cccctgataa cactttaagg aacgttgatt
    16741 tacctgcacc atttgcacca atcaagccat agcagtttcc gggggtaaat ttaatattca
    16801 catcatcaaa tagtttgcgg tctgaaaagt gcaaactcac atcatttaca gtaatcaatt
    16861 tttttcctca cttttatcat tattagtttt gacaagtctt atagaattat ataggggaaa
    16921 ggtaaaagat tcaaggcaat cgaacattct ttgtgtttca cgtgaaacag aatgaaaaaa
    16981 ataaaaataa aagcggagcg aaaatagcta ctataggatt tattagtttg caacagcata
    17041 atttttgacg ttatttataa gagggagtac tataaaactg taaaagaaat gaaagaagga
    17101 ttgaaaatgg atgaaaaaag aaccatcgac tggggctcat tgattttggg aattttattt
    17161 gtacttgtct cattgttatc atttcaagac cctgtcggaa atttagttgc aattgtagta
    17221 gtctttgcga tttttgcctt tattaaaggg atatttgaac tgtttgtacg gaacagatta
    17281 aaagagctaa caggatataa aggaaagatg cccttaataa tcgggatcat cgatattcta
    17341 gttggcgtct ttttcttatt caatattggt gcaggagtcg cagcattgcc ttttgttttt
    17401 gcagtatggt ttattgctga ttcagtttta gcgttattta ctgctgactt ggcaagagga
    17461 gtaagtgaag gatattattg gtttacgatc atcgtcaata ttttaggaat tttattagga
    17521 attttcttat tgtttaatcc aatttcttct gcgttgacat taagcttttt agttggattc
    17581 tattttatgt tatttggtat cacacatatc gtctatgcat ttagataagt aaaatcaaac
    17641 aaataaaaaa gccggcttcc gattacagtt ttactgttgt aattggaagc cggctttttt
    17701 atcatggaca aatatgcacg aggtgatttt taggtgtcga gacaaattta tgcaaaaatg
    17761 tgagaagcta tatacacaaa ttttatctta atactcgaag catagcgcag ttgtcacaac
    17821 ctcttttcat ataattcatt caatactggc aaatcatgga cttcatattt ttctttgaat
    17881 gcttggattt cttcttcaga atattttctt ggatctaaat gcagatcttc tactggcaat
    17941 gggcgcttgt tattgatctt cacatcgatt actacaggac gatctgattt tgcagctgca
    18001 tcaaaggcaa atcgaagttg cgagtattct gtgactgtaa agctatttgc cccgagcgct
    18061 tctccaactt taccgaaatc tgcatcttct aaaaatacac caaatttctt ttgttccgta
    18121 tcttcttgtt ctgcttcgat gaaaccaaaa gaatcattcg aaaagacaac attaataatt
    18181 ggaagctgat atttgacttg tgtaatgata tcttgcatga ccatagcaaa cccgccatca
    18241 ccacttaagg taaatacttg tttttctggg tagcttagtt gtgcggcaat tcctcctggc
    18301 acaccattac ccatagttgc gaaccatcca gatgtcgtat gtttttgttt accgttcata
    18361 ttcaataaac gaatagaatg gatagtcgtg ttaccgacat ctgtgacgaa aatcgcatcg
    18421 tcttcagcaa tacgattgat ttctttatag acagcctctg gacgaatcgg ttcttcctct
    18481 cgatcttcaa aagatttcaa ccaatttacc catttttctt tattttcttg atttgctagg
    18541 taccagctat cagcagggcg agcatcccct aactcgacaa gtctttgcaa agctgtgttt
    18601 gcatcaccga ggatcgacaa agatgtttca tggcgtcgtc caaattttga cgcatcgata
    18661 tcgatttgga taaattcagc agttggatta aaaaatgcac gtccaaatgg gaagtcgcta
    18721 ccgacaaata agatcagatc ggcttctgcc aatgcttcat ttccgggttt agtagcgaca
    18781 cgtccagcaa aaccaagaaa gttttcataa tcatcaggaa taattccttt tgctagaacg
    18841 gaagcgacaa caggaactga aaaatgttct gagaattctt tgatttgcgg aaatccgttt
    18901 cgcgttcctt gaccaacata aagaactggt ttttttgctt tttcaagata aggcaaagca
    18961 gctagcaaat ctttttcttc tggtaatatc acacttgttt tgtgagtgtg agcggtggag
    19021 aaaggttgtt cgtcaatttc ttcaaaacca agatcaacag ggatagtcac gatagccacg
    19081 cctttatgct cataggcggc tttaatggct tcatctacta catgcggaag actttgtggt
    19141 gtcatgactg tacgattgta tacgctaaca tctgcgaaaa taggattttc atttagttcc
    19201 tggaaagagt tatagttcat ggatgtagaa gcaacttgtc ctaagaacgc tactagaggc
    19261 acatggtcca tctgtgcatc atacaaacca ttgatcaaat gtgtcgcacc aggtcctgca
    19321 gagccgaaga cagcgccgac tttccccgtt aattttgcat ctgcagcggc tgcgagtgcg
    19381 ccgacttctt catgacgaac ttgaatgtag ttgatttcgg ttttttcttg atatagcgca
    19441 tccataattg aattgaaaga tcctccggga atgccgtaaa tatggtcgat accccagctt
    19501 tcaagaactt ttaccattgc gattcctgca ttgatttttg acatggtgtt tcactccttt
    19561 agcaatgatg atacataaat tttttgtatc tagagtatag aactttttat cgaaaatccc
    19621 aactaattcg tcttttcaca aaaatgtagt catgtttttg ggaatatgct agagtagtac
    19681 atagatagat tcaagagagg ggaatggcat ggttgaatgg ctattgagac ttgaaccttg
    19741 gcagcaagca cttgctggga cagcttttac ttattttatg acagctcttg gtgccgggtt
    19801 ggtttttttc tttaaagaaa tcaaaaaaga agtgctaaac ttgatgttag gatttgcttc
    19861 tggtgtcatg attgctgcaa gtttttggtc tttgttagat ccggctatta caaaagcaga
    19921 agaaaatgga gatattgctt ggctagttgt aagtattggg tttggtttag gcggtgtgtt
    19981 tttatatatg gcagataaaa cactgcccca tatgcatttt ggaccacaac atgaagcaga
    20041 aggattgcct acccatttaa aacggacgat cttgcttgtt ttttctatta cattgcataa
    20101 cattcctgaa ggtttagccg tgggggtagc attcggtgca gcagcaactg cggataatcc
    20161 gacagccgct atattagccg ctgtttcggt ggcgctaggt attggtatcc aaaacttccc
    20221 agaaggagca gctgtatcaa tcccgttgcg acaagaaggt ctgagtcgta aaaaggcctt
    20281 tgtttacgga caagcttctg gaatcgttga accaattgct ggagtgattg gagcgctact
    20341 agtaacgaaa gtggagctgc tgcttcctta tgcacttgct tttgccgcag gagcaatgat
    20401 ttatgtcgtg gtggaagaat tgatccctga agcacagcag acattgtcca gcaaacgtca
    20461 ttttgcagtt tttggcgtca tgagtggatt tatcattatg atgattttgg atgtggcatt
    20521 aggataaata aaaaaaatat atatgatata tcaaataggg ccgcgaactt atatcgcgac
    20581 cctatttttt tagtaagtta tatcataatg aagtgaatgg tatcatacat taataatagt
    20641 tttttctatg tgtatttcct ttgatccagc gaacaagcca gatgataagt gcgattggga
    20701 ttagaacttt gaccgcaaac cataaaagcc cgcttaggac actgacaaaa agactgatga
    20761 cgaagctagc tgcaattagc actaaaagaa ccatgaagat atttaagaac atattttttt
    20821 gacgcataca aaaccctcct cttgttcatt ctttactata gaaaaagtat aacagggatt
    20881 gtttaaaaac acctccaact gaagactgat ttttacttca tctacttaaa tgatcaaaca
    20941 attgcttttt ctctggttta tcatctaaaa tagagcggaa aaggaggaga atatcatgcc
    21001 taaaaatcgt cttgaagcat ttacagatgc tgtaatcgcg attatcatga ctattttagt
    21061 tttagaactt catgaaccta acggagacac gtgggaggcg ctcgctgcat taggttaccg
    21121 attttttatc tatattgtca gttttatcat tttagcgatt tattggaaca atcatcatca
    21181 tttatttcaa gttgtacata agataaatgg acgggtctta tgggcgaaca gtttctttgt
    21241 ttttgcgctt tcgctttttc cgtttgcaac ttcatggatc agtgagtaca tcaatagcct
    21301 ggctccagaa atgacttatg gttttgttac cttgaccgct aatattgcgt attatatctt
    21361 ggcaaaagaa cttgtcaaag caaataaagg ccacgagaaa gcattgaagg cgatgttgaa
    21421 taattacaag aaatcgtatc tatcgattgg tttgaatgtg ttaggaattg tcttagggta
    21481 tttagtagcg ccagtagccg tgctgatcgc aaacatcgtg attctagctg cttggatcat
    21541 cccaagtcgt accatcgaat cacaatatcg gtaaaatgat ccgtgtttaa aatttaagag
    21601 aaaaatatag aaaaaataaa taaacgatca aatatccgag tgatactagt attcaaagag
    21661 ggatgcagat ggatcaaaaa agaattcatg taggactgat tactagtttt gtcacgatgc
    21721 ttatttattt ggatcaaggt catttgactt ggacagtagt tggtgtcgta ggatttttga
    21781 cattattcac agcaataaaa aaataaacat ctttatgtaa tctgatgttc tatctattta
    21841 agaaaagcat gtttacagca gtatgattct gttgtaaacg tgtttttatt tgtggaaaat
    21901 ttaattttta gaaaagtgta tcgaaaaata gatagttagt ttttccacag tggataactt
    21961 cgtaagagat aaaaataaaa aattagtcaa agaagggcgt atagatttct tttgatagga
    22021 gatatacaac agaaatgaat aaatattaac gtgttttcaa acagtataaa acggtgtggt
    22081 ttttggaata aaaaaataac atatctcatt ttacactttt gttattttat acttaaaaaa
    22141 taacgacaca gaaaataccg tgtctcagat ttctctacta cttctaaaaa ctactgtttt
    22201 tttcctttga aatgccttca aaaagtagga tcaggcagaa tatactggca gaatcaagga
    22261 gaagggagcc taaataagga gaa
//
LOCUS       DAMYXM010000043        22133 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.044, whole genome shotgun sequence.
ACCESSION   DAMYXM010000043 DAMYXM010000000
VERSION     DAMYXM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.044"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            165..1592
                     /locus_tag="PNS21_001797"
     CDS             165..1592
                     /locus_tag="PNS21_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5159507.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1684..2049)
                     /locus_tag="PNS21_001798"
     CDS             complement(1684..2049)
                     /locus_tag="PNS21_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDI5159508.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(2046..2411)
                     /locus_tag="PNS21_001799"
     CDS             complement(2046..2411)
                     /locus_tag="PNS21_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDI5159509.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2603..2851)
                     /locus_tag="PNS21_001800"
     CDS             complement(2603..2851)
                     /locus_tag="PNS21_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159510.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2850..2978
                     /locus_tag="PNS21_001801"
     CDS             2850..2978
                     /locus_tag="PNS21_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HDI5159511.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3035..4729
                     /locus_tag="PNS21_001802"
     CDS             3035..4729
                     /locus_tag="PNS21_001802"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDI5159512.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4733..4852
                     /locus_tag="PNS21_001803"
                     /pseudo
     CDS             4733..4852
                     /locus_tag="PNS21_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4829..5002
                     /locus_tag="PNS21_001804"
     CDS             4829..5002
                     /locus_tag="PNS21_001804"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159513.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5380..6513
                     /locus_tag="PNS21_001805"
     CDS             5380..6513
                     /locus_tag="PNS21_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDI5159514.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6544..6864
                     /locus_tag="PNS21_001806"
     CDS             6544..6864
                     /locus_tag="PNS21_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HDI5159515.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6893..7357)
                     /locus_tag="PNS21_001807"
     CDS             complement(6893..7357)
                     /locus_tag="PNS21_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5159516.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7489..8742)
                     /locus_tag="PNS21_001808"
     CDS             complement(7489..8742)
                     /locus_tag="PNS21_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDI5159517.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8771..9088)
                     /locus_tag="PNS21_001809"
     CDS             complement(8771..9088)
                     /locus_tag="PNS21_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDI5159518.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9114..9434)
                     /locus_tag="PNS21_001810"
     CDS             complement(9114..9434)
                     /locus_tag="PNS21_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDI5159519.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9898..11313)
                     /locus_tag="PNS21_001811"
     CDS             complement(9898..11313)
                     /locus_tag="PNS21_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDI5159520.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11330..12646)
                     /locus_tag="PNS21_001812"
     CDS             complement(11330..12646)
                     /locus_tag="PNS21_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDI5159521.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12796..13494)
                     /locus_tag="PNS21_001813"
     CDS             complement(12796..13494)
                     /locus_tag="PNS21_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDI5159522.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13783..14739)
                     /locus_tag="PNS21_001814"
     CDS             complement(13783..14739)
                     /locus_tag="PNS21_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDI5159523.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14852..15685)
                     /gene="msrA"
                     /locus_tag="PNS21_001815"
     CDS             complement(14852..15685)
                     /gene="msrA"
                     /locus_tag="PNS21_001815"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDI5159524.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15860..17128)
                     /locus_tag="PNS21_001816"
                     /pseudo
     CDS             complement(15860..17128)
                     /locus_tag="PNS21_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            17366..17602
                     /locus_tag="PNS21_001817"
     CDS             17366..17602
                     /locus_tag="PNS21_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159525.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            17609..19561
                     /locus_tag="PNS21_001818"
     CDS             17609..19561
                     /locus_tag="PNS21_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDI5159526.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(19633..20130)
                     /gene="dfrG"
                     /locus_tag="PNS21_001819"
     CDS             complement(19633..20130)
                     /gene="dfrG"
                     /locus_tag="PNS21_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDI5159527.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(20766..22100)
                     /locus_tag="PNS21_001820"
     CDS             complement(20766..22100)
                     /locus_tag="PNS21_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159528.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT         7125 a         4249 c         3710 g         7049 t
ORIGIN      
        1 tttatttttt aatagaatta tctgttttcc aaattttata acataaaaaa ccttttttaa
       61 cggcatttat atgctttttc agaaattaac tgatgatttc aaaaagaaaa catgtcataa
      121 tatttcccat attgtgcaaa aaaatacaag gcggaggctc tactatgatt gaagaatata
      181 ttgagaaaaa tattttaaga cagctgtttt tatgtgggca attctatgta aataaagaag
      241 tcaatctaga aaagctctcc aatctcctgc atgtttgcaa aaccacttta ctcaatgata
      301 tcaataacat aaaaaaagaa tttgaagaac agatcgctta tacacatcgg gaaaaggacc
      361 gttacacttt atatttttca gaacatatac ctcgatgcaa gatcatgcag caattgtctc
      421 aaaattcgtt gtttttaaaa acttgtctgc tttatttgga agaagatgaa cctgattatc
      481 ttcaattaac tgaatgtgaa ttcatctctg tcagcaaagc ttattcatta aaaaaacaag
      541 tattagctta ctttaatgac tgcgggatcg aaatcgaccg ctactcccct cgattcactg
      601 aaatggaaag acgtctgctg cttcttaatg tgtcgtatcg tttaggcggt tttaattctt
      661 gggagctacc tgaatcgttt tttgaacgtg ccgatcgttt cattgaatcc gtaacggaaa
      721 atagtggcag attttatgat aaagaaaata aagaaatctt atcgattggt tttgctatca
      781 gtttcctacg ccagcaagtg tgtgctgtga caatagattc aaaatttatc gaagaaatca
      841 aaaaacgtcc tatttacaat tatgtcgaat cagcatggga aaacactgat ttccaaacct
      901 attataaaaa agaagaattc gcctttattt tgacactatt caatctttgt aattatggtt
      961 tccattctta tcagttgatt gcggaagact ttcaacagct acaccaagtg tttattgaca
     1021 atactcccga aataaaagaa ctagttgcta cctttgaatc tcacttcaat caagaattat
     1081 tcggaaacca accgttcgaa cgtgcgctga ttcatctgat gcgttcggct tgggataact
     1141 atcaattatt catgccagaa aaattttatc ttttaaatga ggaacagaca aatttatata
     1201 aagaagtcca gacaattttt tccagctggt ccagccagtt accttatgat ctacggttga
     1261 atcctaattg catgcgggct tttgtgatcg aattgagcgg aattctccgt ctgacaaaag
     1321 aacatctgac tatctatatc gtcacaaatt cggatgttca ttatctgatt tatcgggaag
     1381 ccttggaagc tgtaactact ttcgattttc aagttgcacc aacaatttat tcaagtatat
     1441 ctgatataaa gaagtatgcg cagcagtcct caaaccgggt attatgtgaa cgtacattat
     1501 atacaccaga tgcggtccaa tatgaaaata ttattcctat ttcgatcaat acgatcgatc
     1561 gagctatcat ttcagctgtg caaaacaaat aaggggctgt gacaaaaatc atgtcacagc
     1621 ctcttatttg ttgaagatgc ccatttctgt aacaatctct tgatacacgt caggctcaca
     1681 acctcactta ttttggacat ctaggggtcc gatctcttta tttaaaaaat aagaaatgat
     1741 ggttcggata actaccaacg ccgctagttg gaaaatatcc tgcaaggtag gtttgataat
     1801 cgattcgatg atcccagctg aaacaagaat ttccaaactt agaagtatat agcctcccaa
     1861 catcttttta atagcattgt tctcgcttac tcggtcgctt tgcacttttg tatggacaaa
     1921 taaatatttc aaaaaatcct tgattgatag acaaacaccc caaaccaata aacagatagc
     1981 aaagatatca agtcccagta caagtttttc gaaatatgga agaagaaact gcatcaactc
     2041 tgtcgtcatc ctacctctcc tcttctttgt ttttctttgt cttctatttc ctgattcaag
     2101 aaataagaaa tgactgttcg aatgataata ataagtgcaa gcttcaaaat atctgtccaa
     2161 gtcggcttga taactgactc gatgatatca gcaacgacta gtacttccaa gcttaacaaa
     2221 atataacttg ctagcagttt tcttgtatct gcattacgag tattacgttc tgcatagttt
     2281 ttcttgacat gagaccaatc aaaaagtttg aatagaacaa gaacgatccc gacaattaag
     2341 ataccgatag aacaaatatt taaaatagca atgatccatt ctagaaattc aattaaattt
     2401 gagcgaaaca tatacgctac ctactttctt ttaatttgtt cctattcttt tattgtaaaa
     2461 gaaagttacc ttgttcgtct atttaaaagt tgttctaatg ataacggttc gcttaaattt
     2521 ttctttttca ctctcccatt tttctccctc catccataaa tcgtggaagt atccaaaaca
     2581 acgtagcaag ttaaaataag gcttagtccg tcatcagctt ttcaaagaag cactgtctcg
     2641 gtgctttttt tgtacccaaa tttctaaaaa tagaaccata cacctcctca aatcttttct
     2701 tcttacctac cctattttta tttacaggaa aatcagacac ttcttctttt ttgtcaaaat
     2761 ttcctttttc ttctcgtcct atcatttggc attctaataa aaacgaacta tccttggata
     2821 tagaaagaga aaacaaggag gcgtttttca tgagtaaaaa attaatcaaa gtcggcatcg
     2881 gtttaggttt gcttgccctt ggcgctgcat atctaggaaa aaagacagga ctattcgaag
     2941 atgacagtca tttatatgat gaattcgaat caatctagca tcctatttag ttgaatcact
     3001 acggacatac ttggaagaca ttggaggaat cacaatgaga aaaagtaaag caataatgat
     3061 tggtgccggt attgccaata tggcagcggc tgtttatttg attcaagaag gcaaatggaa
     3121 aggggatcag atcacctttt attcattgga tgatcacggt tcaaatgatg gtgcaccgac
     3181 cgacactgta gtggatgaat attggaataa aaaccatcca ttagaaaacc aaaaaggcta
     3241 tattgctcga ggcggtcgca tgcttaatta ccgtacctat gtagatttga tggatctgtt
     3301 aagtcgaata ccttctgtta ctgagccagg gatgactgct gaagaagata cgagagattt
     3361 tgatgcaaaa caccgaacct ttgacaaagc acgtttgatg gaaggtggca aaggaatcat
     3421 tgatggcgga aaactgggat tcaataataa agaccgtatt cttctgacta aactgattgc
     3481 tatgcctgat tcagaagaag aaaaactaga caatatcact atcgaagaat atttcaaaga
     3541 tgatccacac ttttttgaaa caaacttctg gtttatgtgg gaaacaacat ttgctttcag
     3601 aacacgtagt tctgctcaag aattgcgtcg ctatatgcat caaatgattt atgaattcac
     3661 tcaaatcgaa catctagtgg gcgtcaatcg aacacgttac aatcaatacg aaagtattat
     3721 gcttcctttg atcgagtact tgaaagaaca aggatgcaaa atcattttga accgccgtgt
     3781 cactgactgg gaattcaaag aaacaccgat gcaagatgaa atcactgtga ctggtttgaa
     3841 aattaccaat acactgactc aagaagaaga atacgttgtg gtcgatgaag atacagcagt
     3901 tattttcact aatgggtcca ttacagattc agctacttta ggagattacg aaacaccagc
     3961 tgtagaaaat atggattatg gtgcagcttc tagtctctgg aaaaaagcca gtgaacgttt
     4021 ctacaacttg ggaaatcctg acaaattctt tgctgatcga gatgcaagcg aatgggtcag
     4081 ctttaccctg acgacgaaaa accatttgtt gttgaatgaa atcacccgta tcacgactca
     4141 agaaccagga aatgccttga attcatttat ttcaaccaca ccgatcacgc cgttaggcca
     4201 aaaagatgtc aatatgtcta ttgtcgtgca tcatcaaccg cattttacgt ctcagaaacc
     4261 aaatgaaaca gttatttggg gttatttcct ttatccaaga cgacgtggcg aatttgtgga
     4321 taaacagtat attaaaatga atggaaaaga aatgctagaa gaactgattg gacaactttc
     4381 aaaagtagat cctggaccag tgaatattcg tgaaaaagaa acagaaattt tcgacagcgt
     4441 aataaataat atccctgtct atatgcctta tgcttccgcg ttgttcaata atcgtgccaa
     4501 aagcgatcga ccaaaagtca ttcctaaaca ttcgaccaat ctggccttta caggagaatt
     4561 tgtcgagcag ccttatcaga tgatctttac tgaacaaagt gctgtacgtt ctggagaaat
     4621 cgctgctttc cattttgcgg gtattcctat gtcccgtctt gtcaaaacac cgcgttacga
     4681 caaagatatt cctactctag ctcgtgccgc aaaaaaaatg tttgaataat caagaggttg
     4741 tgaaaaagct gccctgcatc aagtaataag aacaaccagg ctaaaacagc ttcttatgtt
     4801 tttgatttgg ttggacttat taccgcagat gcaagctttt gaacaccgtt taccaagagg
     4861 ttatgaaaaa gctgccctgc atcaagtaat aagagcagcc aagctaaaac agcttcttat
     4921 gtttttgact tggttagact tattaccgca gatgcaagct tttgaacacc gtttaccaag
     4981 aggttatgaa aaaggcgttt agtttcgagt atgaagaaaa attttgtgaa aatggttcct
     5041 cacgtttttg cataaatttg acttaatacc gagaaactga tcccttctgt cataacctct
     5101 ttttatttat gtctgtttct ttttgaaaac aactattttt tcaacgttta ctctcttgcc
     5161 aatccaacat catctaacgg atacaactat ttcaatgatt accattacac taaaaaatcg
     5221 gaaacacacc tctccgaaca caaatacata agactataga atgtttcatt taagaaaaaa
     5281 agcgtttcat tatcaaaaag gtaagatctc ctttaaaatg aaattatctg tagtactaat
     5341 aatgcagaca attttatgaa tctgaaagga gcatctccaa tgaaagcagt cacttggcaa
     5401 ggaaaacaga aaatggaaat cagaaacgta gatgatccaa aaatattaga accaacagat
     5461 gcaatcatcc gtatcacagc aacagccatt tgcggctccg atctgcatct ttaccatcat
     5521 ggtgaagcag ttttggaaaa agggtatgtt gtaggacacg aaccaatggg aatcgttgaa
     5581 gaagtgggat ctcaagttca aacattgaaa aaagggcaac gtgtggttat cccttttaat
     5641 attagttgtg gtcattgtca tttctgcctg aatcacatgg aaagccaatg tgatgaatca
     5701 aatccagatc agttatatgg cgggcttttc ggatttggca agttgaacgg taatcattgg
     5761 ggtggtcaag cggaatatct aagagttcct tatgcagact ctacttcatt tgttgtacca
     5821 gataatgatc ttcctgatga aaaagtttta tttctatcag atattttgcc cacagcttat
     5881 tggagcgttg aaaacgcagg agtaaaaaaa ggcgatacag ttgtgattct aggttctggt
     5941 ccagtcggtc tattcgctca aaaattcgct gttatggctg gagcagagcg cgtaatcgca
     6001 gtagatccag tacagcaccg tctagataaa gcggctaatt acaatggggt tgaaacttat
     6061 ttcttagaag acacggcaca agctggaaca gaattgtatg aactggctaa aggtggcgca
     6121 gatgtcatta tcgattgtgt aggaatggac ggattagagc ctgtcaaaga aaaagcgaaa
     6181 aatttagtaa gcttacaaag cggcacaatc tctccattac agatggcatc gcaagctgtc
     6241 aaaaaatttg gaacagtcca agtaactggt gtttatatga ctccagcttc ttcttacccg
     6301 ctgcaagaat tttttatgcg ggatatcgct gtcaagcatg gacaggcacc aattatccat
     6361 ctaatgccaa aaatttacgc aatgattgca gaaggactat tcgatcctac acaaatcatt
     6421 actcattcgc tgccactatc agaagcagca caagcatatg agatctttga caaaaaagaa
     6481 gataaaaata taaaagttgt tttgaaacct tgaattcagt aaaaaataca ggtgattttc
     6541 tttatgccca aaatcaaacg aaatacccat gagatcgaaa taggaaaaga agaagatatc
     6601 gaaatacgag gacgtcgctt ccgcttctat ttccaaaatc gttacacatt gatctcactg
     6661 gctgtcgatt tattaaccgg gattttctat atttttggaa gccttgcttc cttaactaaa
     6721 atccccgatc gatatggcac ctatttttat ttggctggtg ctattttctt gacgattcgt
     6781 cctattctca gaatcgtaag gaacattttt atttacgacg aaaaaaaagc aatgaagaga
     6841 acaagcaaaa aaaaagaggc atgagaattt ttcaaatctc atgcctcttt tttcacccta
     6901 tcgtatcact ttgatacaga cgagcggtcg tcccactttc ttccttgacc attgtcaaaa
     6961 atgtccactc gtatcgttca taaaacgtgg tatgatctgt aattaagtaa agtttttcgt
     7021 acccaagttg atgcatttct tttctgattt ccgctaatat ccgcttggct atcccctgct
     7081 tacgcttttc cggttcaaca aacagggcac aaagatttgg tgtcagatca gggcggttat
     7141 ggaagtcgtt ttcaatgacg cccgctcctc cgacgatggt ttctttttca tctaagacca
     7201 cataccattg aggaatactt tccttattag cgattccagt acgaatactt tcctcataca
     7261 caacagcagg aactccccat tttgatgaaa accaattcgc gctttgtttt actaattctg
     7321 ggtgttctct taatttgatc cagttatatg taaccaatgt ttaaacctcc gactaaaata
     7381 aaacgtttac aaaaattata caagtcttta tgaaaattag caatgacaac aatagtgatg
     7441 taaaaaaaaa aacggaaaac aatcacttgt tctccgtttt ttgattaatc aagcatctaa
     7501 agcttctttt cttctttctt ggatcgtagc taccttgacg aacggtagat agatcaaaat
     7561 agaaagaacc aaattgacag cggctagtac accacctgca atactttttg ttgccaatac
     7621 accgccaata attggtggag ttacccatgg aggcataacg gtagctgctg gcactaaacc
     7681 tgtagaagtc gcaaagtaag caacactaac taagaccatt ggtgtcaaaa taaatggaat
     7741 gaacatgatc gggttcaaca cgataggtaa cccaaacatc gttggttcat taatattgaa
     7801 gacacctggc gcaatcgaca agtttgttac aaccatatat ggtttgtttt tacgtcctac
     7861 taaataaata gctaacagta aacctaaagt tgcacctgtt cctcctaagt tgacaaagct
     7921 gtcaaagaaa ggtttgttca cgataaacgg tgcgctatgt ccagctgcga ttgcattaac
     7981 gtttgcttca atggctggcg cattgattgt ttgcatcaaa ggatcgacca tattcgctcc
     8041 gtgtaaacca aagaaccata agaatggtgt gataaatgca agcagtaagg cagatggata
     8101 agagtttgca agtcccataa atggttcttg gaccactgta tagaaagaac ctacgatatc
     8161 atcaacaccg aatgcagcaa aaattgcagc aactaaagca aacgcagaga tagtgatcat
     8221 tgctggtagt aatgaagcaa atgcttttgc aactgccggt ggcacaccat caggcatttt
     8281 gatgatcaat ttttcatttc cgcttaaacg tacaaagatt tctgttgaaa taatcccaac
     8341 aataatagca ataaacaaac cgtttgagct catacctaat gctccgccta aagcgaagaa
     8401 agagcctaaa gaaatcacac ctgcggaaac accgtctttt cggtagccgt tagctaaatg
     8461 ataagcaagc aagaaagcaa tcaagacaga aagaatggca aatgtaccgt tccaagcagc
     8521 agccccgaat cctttccatg attcaccacc aaaaattgag ttcatcaatt cttggaaccc
     8581 gggaatcggt aagttattga tcattactgc taatgctcct aagatcatta acggcatact
     8641 caccataaat gagtcccgaa tcgcaactaa atgccgttgt gcaccaattt ttgatgctac
     8701 aggtataaaa tgtttctcca taaatgcaac aaatttatcc ataaatcttc cctcttttct
     8761 ttactgtaga ttacgcgttc aatcgacgat acagatcaat gatctctttt gctaaatctg
     8821 taaaagctat agatgtcatc agatgatctt gactgtggac cgtcaaaagc gtgacttcaa
     8881 cgtgatttcc ttgtgcttct tgtgtcagca tctctgtttg agagtgatgt gcttggacta
     8941 aagattcctc tgcatctttg attttttcat ctgctaaagt gaaatcacct tttttcgcag
     9001 cagcaatcgc ttccattgca tcgctttttg catttccgcc aaacatgatc agacccatga
     9061 tggcttctag atttttttga tcttccattt attgtatcct ccgataattt ttattatttg
     9121 tttattagtt tttctgcttg gtcgagtact ttttcaccat tcatcatgcc atagtcagcc
     9181 atattgatta catctaaagg aatgccttta ggtgctagct tttcagcaaa ttgattttgc
     9241 ataaagcgta cttgcggtcc taaaagtaaa acgtctacat ttttagattc tagttgatta
     9301 tccgcatctg aagcagatac agcaaagatg tctgcttcca ttccttttgc ttctgctgcc
     9361 ttttgcattt ttgtgactag gagacttgta ctcattccag ctgaacatac aagcatgatt
     9421 gttttctttt ccattttaaa cacgttcctt tctatactta cacttgtata taagcaagta
     9481 gcgtgccaac tttaaaagcg ctttaaaaag ttgatatcat cacatttaag gtctatacca
     9541 agttgcatac acactaaaaa agaaagcgca cactaaacac acaaattaat ttttattacc
     9601 ttttttcttt taacgtgcac aaaaaaagtt gtgacacaaa acttgtcaca acttttttca
     9661 aataaacggt attcaaaagc ttgcatctgc ggtaataagt ccaaccaagt taaaaacata
     9721 agatgttgtt tttactttgc tattcttatt acttgatgca ggacagcttt ttcacaacct
     9781 ctcaataaac ggtgttcaat cagctgaccc tgggtattag ctcaaaacgc tggattcaca
     9841 acctctttta tttcaaataa ccagtaaata caatgaacat ctacaaaaaa cttctattta
     9901 tttatagatt tcgtcaggca aatccaattc tctcttatca atcaaagtct gataccacga
     9961 agcagatttt ttcaatgttc tgcttcgttc atcgttcaat tcgattcgaa ctaatccata
    10021 tcgattctta aatgcattca ttggtgacac acagtcagtg aatgcccata acatatagcc
    10081 ttcgcaattt gcgccttctt ctgttgcttt gatcaagtaa tgcaaatgct gtccgataaa
    10141 ttcgatacgg taatcatctt gcacgattcc tttctcatcc atatactgtt cttcatattc
    10201 tcttcccata ccgttttctg ttatcagcca agggatatta tgataatttt cttttagata
    10261 catggctata tcatacatga ttttcggata gatttcccat cccctggaat tattcatttt
    10321 cttcccaggt aaatcaaaga cttcataata tcgttcagga tggaaaggtg tctcttcatt
    10381 ccattgataa cgaggggcgc gaacgcgttt aggataatat tgattgattc caaggaaatc
    10441 tactgtatga tgctgaatcg tttctaaatc ctctgttgca ggatcgaaat aaatcgattg
    10501 tttttgacac aactccagga actcttctgt gtagtatcct tttaccatcg gatcaaaaaa
    10561 tactcgatta taaaacaaat catacatata agcagctttt tggtcttctg gagaattgga
    10621 tcgagcatag accatttcag gatttaaaat acagccaatc cttccaccat aatttccgct
    10681 gcggtaaatc tctactgctt tagccgttgc taaaacttta tgataattcc acatcatcca
    10741 cttttttgtg ttttgttcat gcggccagcg aacagcatcc aaatagcaac gcgtttgggg
    10801 aacgattggt tcgttgaatg taaaccagtt ttttacccta tcaccgtaac gatcaaaggc
    10861 aatttgagca tacttagcat aaaaatcaac tacttttctt gaagaccagc catcatattt
    10921 ttccaaaaga aaagctggga cttcataatg ttccaaacaa atcatcggtt ctacgtctgc
    10981 ttcgatcaaa gagtcgatca tttcacttat atgctgagca tattcttcat ctactataca
    11041 gttctcataa tcagtaaaaa aacgagccca attgatagaa gtccgataat gtgtcagatg
    11101 gatggatttc atcagatcca catcctgctg gtattgttcc ataaaattag ttgctacggc
    11161 tgggccatac ccattatgcc aaacaaaatg ttcattttta taccagctat ctataaacga
    11221 atcctgtcct tctttttttc ctttccaacc ttccgtttgc caagcagaag atgcagcacc
    11281 aagaataaaa ttttttggaa tagtaatttt catgatttcc tcctgttttt tagatcccat
    11341 cattttccac acgatttgct acttttacaa atggcagata gatcaaaata gaaacaataa
    11401 tacaaacaag ttgcgtaata actgcaccga tatttcctgc tgttgccaga taagcattga
    11461 cgatcggcgg cattggccaa gggaccatta caactgcttt cgccgcaaat cctaaagcgg
    11521 ttgcaaaata accaatcgtt ccagtgataa gaggcgttaa gataaacgga atagctaaaa
    11581 ttggattcag catgatcggc ataccaaaaa taactggttc attgatgttg aagatcccag
    11641 gagcaaggga taacttggtg atttgtttca tatcttcccg ttttccaact aacaatactg
    11701 caatcagtaa accgatcgtg actccagacc cgcccatact catatacata tcccaaaatg
    11761 gcatagtaat aatgttcggt acttcttttc cagcttcaaa agcttccgta tttacagcaa
    11821 ttgccgctaa aagcaacggt tcccgtatcg gcttcaccat ttgatttcca tggataccaa
    11881 tcacccaaaa gacttgcgca ataaacatca atagcaaaat acccggtaac ccttgtacga
    11941 caccttctaa tgggcgttgt acgacattgt aaatgatatc ataaaggtac attcctgtaa
    12001 gttctttgat gataaatccc gcagccgcga tacccgatac agtcagaatc gtcgggaata
    12061 aagcagaaaa actagcagaa acattcgttg gcacggtatc aggcattctc agctgtagtt
    12121 tcttctgttt cctcaaccag caataaagtt caatagaaag gatggagacc aacattccca
    12181 aaaataaccc tttcgtatct gtatattgtt tagccaatac gttttctact ataacagtct
    12241 gattttcatt cactaattca taaacagttg gattaacaga taaataagac atcactgcga
    12301 tgataccagg gaatacacca tccactttat tcagcttggc taattcgatc cctatcagaa
    12361 aaacagcata gatggtcaag aaacttagtg tgacatagct gatggcagat gcgataggct
    12421 ttaattcttc taaaaaacgc aagaaactta tctgtgctaa gccattttcc gagtcaaaga
    12481 ccattgctga aaacaatgtg ccaaaagcac ccgtaataat taccggaatc aatgcagcaa
    12541 atgcgttttt tatcaccatg atatacctta aattattgat tctcgttgcc acgttcccta
    12601 aaacattggc taatttatcc ataaaactca ttccagatcc cctcattcct gcttcttctc
    12661 cttgacgtca tctctttttg tctattagtc agcgcttaca tttcgatgaa cagaaactat
    12721 ttactcctct tttttaccaa atataaaaca aaaattcatc tgatgtattt atcataaaat
    12781 acatcagatg aactgtcaat tagtttcttt ttctcctatt attttctgta attctctccg
    12841 atttattgcc atatggacag tcattgcatc aaacgcagca agtgcattcc gcctttcgat
    12901 ggcctgaacg atctctgtat gaatatcgat cgtctcgatt ttcagatgct ggatatcata
    12961 atttttatta aacaaagcaa ttgattcatt aatgattggg ataatatgca gcaaggctac
    13021 gtttccgcta gcttctgcaa tcatcgtatg aaaatcgatg tctaattgaa catgttcttg
    13081 tgtgccttct cgaaaagaag tttcaatctt ttgcgttaat tttttcattt ggacgatttg
    13141 attcttcgtt gcatattccg ctgccaaacc tgccatctta ggctccaata aatatctcag
    13201 ttcaaataag tctcgtatca tccgatcctg atcttcaatc aaagaaaaac cgagtgggtc
    13261 attggcaatc cctcgcttat cactgacaaa tgtccccgct ccttgccgaa cttcaaggat
    13321 attcctggaa gctagagccc gcacagcttc tcgcaccgtg cttcgcccca cctctaaata
    13381 atcggacaat tcatattcat tcggcagttt atctcctact tgcaactgca tttcctttaa
    13441 atatctaagg atcttgtcag tagttacttc tactagggtt ttattttctt ccaaaccaca
    13501 tacctccttt tcatctgatg tattttttcg taattattat aacacgtttg aatcatctga
    13561 tgattttttg tatacaaaat tgattaatcc gttcatagac atacaaacga agaagatatt
    13621 taccataaaa aagactagga taaaattcac tcctagcctt cgatcatttt tattttgaac
    13681 gacaaaaaat atgatgttca ataacagacg atcttcattc gattcttgat gtcatctttt
    13741 gtttttttta atatatggat aaaaagtttg attacatcgt ttctattcta gaatttggtc
    13801 gattctcaat tcttcttctg gtgagaaatc taaattatcc atcattcgaa cactttcttg
    13861 taactgactt aaccgacttg ctccgaccag tacacttgta acagattttt gtcgcaaatt
    13921 ccatgcaagg gccatttgcg ctaatgattg tcctcgtgat acggcgattg tttgcagtgc
    13981 tttgattttt tccagagttg atccaacttg ttcttctgaa aggaatggta tttccttacg
    14041 atgtgctcta gaatcttcag gaataccatg tagataacga tttgtcagca acccttgagc
    14101 aagcggactg aacgtgattg cccctagccc ttcttcttct aatactggag atagatcgtc
    14161 ttctagtagt gcacgagaaa acatattgta gcgcatctga tgaataatga acggtgcttc
    14221 ctttcttttt aaaatttcag tcattttttt tgtatcttct ccattatagt tggatatacc
    14281 aatatataat gcttttcctt gtcttacaag ttgcatcaaa gcttctgctg tttcttccaa
    14341 aggtgtatca gggtctgggc gatgatgata gaagatgtca acataatcta gccccattct
    14401 ttggagactt tggtcacaac ttgaaatgat ggatttacga gagccccatt ccccatatgg
    14461 cccttcccac atatgataac ctgctttgct agaaataatc agttcatcac ggtaggcttg
    14521 aaaatcttct ctaaaaatac gcccaaagtt tttctctgca gctccagcag ggcctccata
    14581 attattcgca aggtcgaaat gggtaattcc catgtcgaat gcgcctctta aaatctctct
    14641 ttgattatgg ataggatcat aatcaccaaa attttgccat aatccgagag ataaaatcgg
    14701 taatttcaaa cctgaatgac cggatttacg atattccatt ttttcataac gtgcactatc
    14761 tgcaatatac aatttttctc ctcctcatca aatcttttta aaacgctttc ttttttctat
    14821 ttttagttta ctacatttta aagaaaagga gttaatcaaa atgatttttt cgtatatttt
    14881 tttgccattt gttttttact cgttgataga tcagaaattg attacgtgct tgctggattt
    14941 ttttataacg tttcggttgt tttttgtaaa attgctgatg atagttttct gctggccaaa
    15001 acgtaccggc tggttcgatt tttgtgacga ttggttggtg gaaagttcct gaactgtcta
    15061 gtttctgttt tgattgttcc gctaattctt tttgtcgttc gacttgataa aaaatcactg
    15121 gacgatattg cttccctcta tcttgaaatt gtccaaatgt atctgtggga tcagtaattt
    15181 gccaatagat tgtcagcaac tcgctataac ttatttctct tgtgtcataa atgatctcca
    15241 cagcttccac atgacctgtg taaccaccac ttacttgatc ataatttggt ttttcaacat
    15301 gtccgcccgt gtatcctgac aaaacagata ggatcccttt tttcgattca aacggctcga
    15361 ccatacacca gaaacatccc cctgcaaata ctgcacgttc ttgatagtca gcatcttgca
    15421 tggcatgctt ggaaaagtca tatttttgct ccttatcaaa tccttccgta atcaataaat
    15481 aaaagtccat tacatcaggc gtcagattca ttcggatagc taatggacgt agtgttgctt
    15541 ctagttttga taactgttct ttaacggata tattttctct tatgtcattc tttgtctgaa
    15601 tcaatacttt tcgttcccaa tctcgtgtat tcggattcaa aatcagatta taaagtgttt
    15661 gaatgaatat ttgttcctca tccatagatt ctcctccttt ccgaagaaga caagctagtg
    15721 actttagctg taatttatca taaagtgagg aaataagcga ttacttcatc cccctacaga
    15781 tttttcgtat aaaaaagaga gaatccttat cgacagattc tcctttttaa tcaatttatt
    15841 ttataaatga aaagttaatt taccttttat ggaaaactat tttaaaaaat aaacataaat
    15901 atgcagttaa tgtaacaaga taagaaataa tagtcgttac tgtataactt gtgcttactt
    15961 taatatcagc aactcctttg actccgctta tttgtaccaa tccattagca ttcacctgct
    16021 cctttacagg ttgatgattt atttctacat tcgttcctaa ataagcatat acaggaagtg
    16081 tgattacttg gctcttctca ttagttactc tatatatgat agaactacca gttgttttat
    16141 aaggaaggtt tacccattga ctgccaatct ttgcttcgtg ttttattata tgctccttat
    16201 tctccaatga agcgatagga gaataatcat ataaaaaata atccattcct ttagtggtat
    16261 acgcctctac tgtttctttg tttacaacaa tatgtcccgg ctgagaaatt attttttgat
    16321 tgaaattttt tttactagaa acagttaaag tgattaataa caaaacagtc ataaataaat
    16381 aaaatagttc tcttgtctta ttgtatcttc tatttttgaa aaatatcatc gttaatatca
    16441 gagaagagaa tattatttgc aagcttaaga tccgccaagg aaattgtaac aatttggctg
    16501 gcgtattttg cagaagatac catggcacca aactagttgt agctagccaa gtcaaacacg
    16561 taccataata aatctttttt tctatgtttg ttaatttttt aaataaaatc aggggaatac
    16621 ataaagataa cagaccaact agtcccattg agtaagtagt taagtcgttg ttcatagctt
    16681 cagtcagact atcgaacaca ttagatgcat gagcatataa atttgtcacg ctaggtttgt
    16741 tgattttttg gtagagcatt tgttcaaaca tcggatacca aaaagcactc gtcagaacca
    16801 atgtcattcc tgccgcacta aataattttt taaatatagc aataaaatct gcattcttga
    16861 aacctttaga aaccaaacga ataaaaataa aaatgataat taatgctgta ctcattaatg
    16921 ttgatagtac atggctgtac actagcaaac tcatactcaa tgctagcttt acagacggat
    16981 acttttcttc ttttccagag atgatttgat aagcataaaa taatacaggg ataacaagtg
    17041 ttattgcgat agcttcggca attgcggaac gatagtagat gtctactgtc cgataagcag
    17101 agaaaatata gagacaacta aataaaaata agtagttcag ttttggagta caaaaagtga
    17161 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
    17221 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
    17281 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
    17341 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
    17401 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
    17461 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
    17521 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
    17581 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
    17641 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
    17701 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
    17761 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
    17821 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
    17881 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
    17941 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
    18001 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
    18061 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
    18121 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
    18181 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
    18241 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
    18301 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
    18361 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
    18421 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
    18481 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
    18541 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
    18601 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
    18661 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
    18721 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
    18781 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
    18841 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
    18901 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
    18961 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
    19021 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
    19081 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
    19141 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
    19201 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
    19261 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
    19321 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
    19381 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
    19441 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
    19501 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
    19561 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
    19621 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
    19681 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
    19741 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
    19801 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
    19861 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
    19921 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
    19981 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
    20041 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
    20101 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
    20161 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
    20221 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
    20281 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
    20341 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
    20401 cttaaaataa aaatgccgct tttttttgtt ttaaaaaccg ctcgccataa tgataagcta
    20461 cgagtgttgt acacaagttc aataaccaaa cgtaaataat atagcttaca atcaaatttt
    20521 ttgtcatcca atataacatt acagctggaa aaaaagtcag aaagggataa aaataattta
    20581 caccatatcc agtataattg aatgtctcat aattaatcgg actggtaaaa atagtcgata
    20641 accccttgat tcgagataag tgaaaagaaa gatctccagt ggtataaata ctctgtgtat
    20701 ttttgaaaaa gatgaaagaa tatatactac taactataat taatccactc aatattacta
    20761 cttttttatt tattttcatt cttcttgttt atctcctttt tagtcacata tcgagaatct
    20821 ttcaacttat taggtgcaaa tcgtttaaaa cttgaataca ccactaaaca ataagtcaga
    20881 atactgatcc ataaagccat gagagcagtt ggagtatatt ttgttgtaat ttctatctta
    20941 ttttctccag catttacatc cacattcact aagccatgtt ttttataaga gtcatttacg
    21001 actttaccgt ttacttttac ctgtgtacct aaataagcat atactggtac tgtcactttt
    21061 tgctttttgt tgcttgccac tgtgtaagta atgttattat ctgcagcttt atacttccct
    21121 ttttcccacg attctcctac ttttacttca tgtttttcta agtgaggcct ttcttttatc
    21181 gcttctgttg gggcgtaatc gtataatcct cccatatctg atgttgtata acgtgggagt
    21241 gtttcttcat tgataacaat atgaccgtgt tttgattgga taacttttgc gtaattttct
    21301 tttgctgcta tagacgtgac gagtagaaac accacagtag cgccaatgat taaccatgaa
    21361 tattttttat tcatagtctt tttcataaaa acaagcgtta tgatcattga agaaaaaaag
    21421 atttggatac ctaagatacg ccaaggaaac tggagtaagc ttatcggtgt tttttgaaac
    21481 aaaaaccatg gaaataaatt agttacgcat acccatgtag aaactacgcc aacatagatg
    21541 attttttctt ttttctccat ttctttcagt atgaataaag gaaaaatcaa cgcaaccaat
    21601 cctaataacc ccatagtgta tgttgtaata tcgttattta acgcttgaat gatactatct
    21661 gaaatagaaa gtgcttctag ctgcaaatct gttttgaagg gactgtgtat ttcttgataa
    21721 ttcatctgct gcaacatagg ataccaaaaa tagctagtta atgcacaagt caaaaaaaca
    21781 gcctttacta aattcacgac cacaaatttt atatcgtttt ttatttgctt gcttaaaaac
    21841 aaacgaacta taatcataca agcaatcaat gcactacaca atagtgtagt taagacatga
    21901 gtatacacaa gtagacttat actgacagat aaagcgatga cttttttttc tctattgaat
    21961 atcaatccat aagcataata taaaacagct gggataatcg taagagcgat tgcttctgca
    22021 attgcggatc gataatagat atcaactgtt cgataagttg caaaagtata taaacaacta
    22081 aataaaaaag cagcatctat ctttttaaaa aacttttctc cataataaaa act
//
LOCUS       DAMYXM010000044        22055 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.045, whole genome shotgun sequence.
ACCESSION   DAMYXM010000044 DAMYXM010000000
VERSION     DAMYXM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.045"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            628..1161
                     /locus_tag="PNS21_001821"
     CDS             628..1161
                     /locus_tag="PNS21_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HDI5159529.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1651..2175)
                     /locus_tag="PNS21_001822"
     CDS             complement(1651..2175)
                     /locus_tag="PNS21_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5159530.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(2168..2641)
                     /gene="tsaE"
                     /locus_tag="PNS21_001823"
     CDS             complement(2168..2641)
                     /gene="tsaE"
                     /locus_tag="PNS21_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDI5159531.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(2764..3744)
                     /gene="pta"
                     /locus_tag="PNS21_001824"
     CDS             complement(2764..3744)
                     /gene="pta"
                     /locus_tag="PNS21_001824"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDI5159532.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(3897..4577)
                     /locus_tag="PNS21_001825"
     CDS             complement(3897..4577)
                     /locus_tag="PNS21_001825"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDI5159533.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4703..5572
                     /locus_tag="PNS21_001826"
     CDS             4703..5572
                     /locus_tag="PNS21_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDI5159534.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            5598..6113
                     /locus_tag="PNS21_001827"
     CDS             5598..6113
                     /locus_tag="PNS21_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159535.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(6160..6753)
                     /locus_tag="PNS21_001828"
     CDS             complement(6160..6753)
                     /locus_tag="PNS21_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5159536.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(6778..6957)
                     /locus_tag="PNS21_001829"
     CDS             complement(6778..6957)
                     /locus_tag="PNS21_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159537.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(7034..7543)
                     /locus_tag="PNS21_001830"
     CDS             complement(7034..7543)
                     /locus_tag="PNS21_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159538.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(7639..8019)
                     /locus_tag="PNS21_001831"
     CDS             complement(7639..8019)
                     /locus_tag="PNS21_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI5159539.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            8349..9098
                     /locus_tag="PNS21_001832"
     CDS             8349..9098
                     /locus_tag="PNS21_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HDI5159540.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(9282..10559)
                     /locus_tag="PNS21_001833"
     CDS             complement(9282..10559)
                     /locus_tag="PNS21_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159541.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10638..11282)
                     /gene="pcp"
                     /locus_tag="PNS21_001834"
     CDS             complement(10638..11282)
                     /gene="pcp"
                     /locus_tag="PNS21_001834"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDI5159542.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11481..12425)
                     /locus_tag="PNS21_001835"
     CDS             complement(11481..12425)
                     /locus_tag="PNS21_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDI5159543.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12427..13113)
                     /locus_tag="PNS21_001836"
     CDS             complement(12427..13113)
                     /locus_tag="PNS21_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDI5159544.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            13587..13907
                     /locus_tag="PNS21_001837"
     CDS             13587..13907
                     /locus_tag="PNS21_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDI5159545.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            14061..14966
                     /locus_tag="PNS21_001838"
     CDS             14061..14966
                     /locus_tag="PNS21_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI5159546.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            14963..15736
                     /locus_tag="PNS21_001839"
     CDS             14963..15736
                     /locus_tag="PNS21_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDI5159547.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15917..17341)
                     /locus_tag="PNS21_001840"
     CDS             complement(15917..17341)
                     /locus_tag="PNS21_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI5159548.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            17552..17998
                     /gene="nrdI"
                     /locus_tag="PNS21_001841"
     CDS             17552..17998
                     /gene="nrdI"
                     /locus_tag="PNS21_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDI5159549.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(18063..20045)
                     /locus_tag="PNS21_001842"
     CDS             complement(18063..20045)
                     /locus_tag="PNS21_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDI5159550.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(20059..20955)
                     /gene="murQ"
                     /locus_tag="PNS21_001843"
     CDS             complement(20059..20955)
                     /gene="murQ"
                     /locus_tag="PNS21_001843"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDI5159551.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(20958..22019)
                     /locus_tag="PNS21_001844"
     CDS             complement(20958..22019)
                     /locus_tag="PNS21_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDI5159552.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
BASE COUNT         6419 a         4629 c         3926 g         7081 t
ORIGIN      
        1 gtttattaag aggttgtgaa aaagctgcac tgcatcaagt aataagaaca gccaaacaaa
       61 aatagttcct catatttttc gtttggttgg gcttattacc gcagatgcaa gcttttgaac
      121 accgtttatt aagaggttgt gaaaaagctg cactgcatca agtaataaga acagccaaac
      181 aaaaatagtt cctcatattt ttcgtttggt tggacttatt accgcagatg caagcttttg
      241 aacaccgttt attgagaagt tgtgaaatca gcgtcctgac ctgagtatta gagaaacaaa
      301 tggcaaaata gttcctcata ttttgctatt ttttgagcta ataccgaggg tcagctgatt
      361 gaacaccgtt tagtggagaa gttgtgaaaa agtcgcctac tttcgagtcg taagaaattt
      421 ttttgaaaat ggttcctcac aactttgcat aaatttgact taataccgag aagtcatgat
      481 ccccatctaa ctccaaatca caaggaatga gttcaaaaac tagcttttct caacaaatat
      541 tacataaaaa attctatgtt ttttgttaaa tttatttgct tttttcaaaa gtatgttaca
      601 gtaatttccc aaggaggttt tacagaaatg aattttgttg ccatggattt tgaaactgcc
      661 aatcatcagc cttatagtgc ttgttctctt gcgctagtca tggtaaaaaa tagccagatc
      721 gttgatgaat tctatacatt gatacagcct gagactcctt ttttctggcg aaatgtccaa
      781 attcacggaa tccatcaaga agacgtacgc aatgcgccga aattcccaga agtctggcaa
      841 actatccaac catattttca aaaaaatcgt ttagtcgttg cacataatgc tgcttttgat
      901 acaaaggtct tagcgggatg cttagattat tatcatcttg aacagcctaa ttatctttct
      961 ttatgtacag tgaaaagtag ccgtcgcctg tttcctgaat ttccaaatca tcgattgaac
     1021 actgtgtgcg aaaatttgaa catccaatta gatcatcacc atgatgcatt ggaagatagt
     1081 cgtgcatgtg cggaaatcct gctttatcaa gaaaaaaaat tcgggacaga tccattgaaa
     1141 aaacttgtga ctataaagta aaagaatact tatgaaacac acaaagaggt cgtgagcctg
     1201 ccgtttcact ccgagtcaca aggagcaatt tcaaaaaata gcttctcatg tttattaaat
     1261 attgtgactg gtgaccgagg agctggcagg agaacaccgt ttattggaga ggttgtgaaa
     1321 aagctgttct gcatcaagta ataagaacag ccaaacaaaa atagcttctc atatttttca
     1381 tttggttgga cttattaccg cagatgcaag cttttggaca ccgtttacca agaggttgtg
     1441 aaatcagcgt tttgacctga gtattagaga aacaaacggc aaaatagttc ctcatatttt
     1501 gccgtttttt gagctaatac cgaaggtcag ctgattgaac accgtttatt caaaaaaaat
     1561 ggactgcgac acgacttttg tcacagtccc ttcgtgtgtt tttctgcctt ctcaaaagga
     1621 tgacagtttc tcactctttt cctatttctt ctattcaatc aaataactca ttttatatac
     1681 atctaaaaat tcacctagat ctgttcttgc ccctctaggc atgaccgctt cgatttggaa
     1741 gcccattttt cggtaaaggt gtacggctct ttcattacga acttgaacgt caagctccaa
     1801 tcgaaaaagt acacccattt ccttcgccca actgatgatt tcttctagca taagagtgcc
     1861 tagtcccatt ccccaatatt cctgcagaat actgataccg acttctccca cgtgagacaa
     1921 acgaaattga ctgtctgcct tgacagatgc tgttccaata atcgtccctt catagattgc
     1981 aagcagcaat aaattattat tgctttcgta tatataatcc aactcttctt ctaaggtttc
     2041 tggtggtaac aggagttctg cttcatccaa aactaaaaat tctgtctcct tattaacttg
     2101 tcccatcatc aaaagaatgt cttttgcatc tcctggcact gcttcccgca agatgatccc
     2161 aacttcttca ctcatgattt tcagcccatt tttctagaat tcgctgtttg aaggtttcac
     2221 tttgctcccc gtatgagatt agtttcacat accgcttttc taaatctgta tcactttttt
     2281 ctaaaatcag ttccaaataa tcctccggca gtgcttcttc taatagattt ccccattcaa
     2341 caacggaaag cccatcacct tcaaaatatt catctaaccc taaatctgct ccgctagctg
     2401 ctacccgata aatatccata tgatacaacg gaatccgacc ttgactatat tctctaataa
     2461 tcgtgtatgt cggacttttg atcatttgcg ttatccctaa tccttgggca attcctttcg
     2521 tcaacgttgt tttacccgca cctagatcac cagtcaatat cagattgtca cctggcattg
     2581 cggaatgacc aattgcctct gcgaattgtt ctgtcattgt taatcctgat agttcgatca
     2641 tttttcatct tctcccgctc ttctttcttt ttgccagtat atcgcgtaat gaagtttttt
     2701 taaagaaaaa atgaactagt caagaacagc agctgctgtt tttggctagt tcgcgtgtat
     2761 ttgttaatca atcaatgttt gtgctgctgt gataatcgat aatttatata catcttcttc
     2821 atttgctcca cgagacaaat cagaaaccgg tttgttcaag ccttgtagaa ttggtccgat
     2881 tgcttcaaag ttaccgaaac gctgagcaat tttatagccg atatttcctg actgcaattc
     2941 tggaaataca aagactgttg cttgaccagc tacttctgaa tctggcgctt tcagttgtgc
     3001 aactgaagga acataagaag catcaaattg caattcgccg tcgatcatgt attgtggcgc
     3061 tagtttttta gcgattttag ttgcttcaac tactttgtct acttctggcg atttcgctga
     3121 gccttttgtc gagaagctca tcattgctac ttttggttcg atatcgaaca attctgctgt
     3181 ttttgcagaa tctacggcga tttcagccaa ttcttgcgca gttggattca cgttgatcgc
     3241 acaatccgaa aagacatatt tttcttgatc tcttccacgt accatgatca tcgctccgct
     3301 tgtccgactg atgccaggct ttgttttgat gatctgaagt gctgggcgaa cagtgtcgcc
     3361 agtcgaatgg atcgctccag agaccattcc gtctgcaatc cccatatacg taagcatcgt
     3421 accaaagtaa ttcacatctt ttaaaatctt ttctgcttgt tctttggatg cctttccatt
     3481 tctacgttca acaaaagtat ccaccatttc atcccactta tcgtaattat cagggctgat
     3541 gatcgtaaag ctagatgttt tgattcctct ggcatgcgct gcatttctta tttcttcttc
     3601 gttccctact aaaatgggtt ccacaagttc ttctgctttc aaccgggcag ctgcgcctaa
     3661 aatacgagca tcagtagctt ccgggaaaac gattttgatc ccacggcgaa caattttaaa
     3721 tcttaaacta tcaaacaatt ccacaagatg acctccagac gtttttatat gtattatcat
     3781 acactattcc agaaaaaaag aacagtttat tttatctgta tcagtcaaaa atgctggaaa
     3841 aattaagttt ttcacataca aagcgaactg cactataaca ggaaagcgct gttttattac
     3901 actgtttcag gcagttgcca atcaatcggt tcttctccta acgcaaggag cgcatcattc
     3961 gttttcgaga agggtttgga accaaaaaag ccacgacttg cagataacgg actgggatgt
     4021 ggagatgtta tgatgatatg gcgactctta tcgatcattt tgattttttc ttgtgccggt
     4081 tttccccaca agataaacac gattggtttt tcccgttcat ttaatttttc aatgatcgtg
     4141 tctgttaatc tttcccaacc tttcccgcga tgagaatacg cttgcccttc ccgaacggta
     4201 agaacggtgt tgagtaaaag gactccctgt ttagcccatg acactagatt tccatgttga
     4261 acaggtgcga ttccaagatc atcataaagc tctttataga tatttcttaa cgatggcggg
     4321 atctttacgc caggttgcac agaaaaagat aagccatgag cttgattttc tccatgataa
     4381 ggatcttgtc ctaaaatcac cactttaact tcttcataag gagtcaattc caatgcttca
     4441 tagatatgat acatatctgg atggatcttt tgggacgcat attctttttt taaaaattct
     4501 cttagatgca gataataatc ttttgaaaat tctgaggaca aaacttcttg ccaactgtta
     4561 tgaatgattt ctttcaaaaa ctccacctcc taataaaaag aataactcac attttcttca
     4621 atcacaaggg acaatcttca ccctcagcac ttgattaata tggtaaacta ggaacaagaa
     4681 gcagaaagga acgataatca ttatgatcaa attaattgca tcagatatgg acggcacatt
     4741 attagatgcc catatgagtg tttctcctga aaatgccgag gcgatacgtt ttgcaaatga
     4801 tgccggtgtt gagttcatgg tcgctactgg tcgaaactat caagaagctc gcgcagcttt
     4861 agatgaggta ggaatcgatt gtgcgatgat cactttgaac ggggcacagg tatttgataa
     4921 agaaggaaat tcattgttta ccgtgtcgat tccaaataat caagcaatct ctgttttaga
     4981 tattctagat gctaacggta tttactatga agtagcaacc aacgatggtc tttattctga
     5041 aaatcaagcg aaacgaattg aaagttttgc ttccatggtg gctacccatt tacctcacct
     5101 tacgtataaa atggcgattg ccatggcctc agctaagctg gagttattgc acatcacata
     5161 cgtggacagt attcgtgcta ttttggaaga aaagaatctg gaagttttaa aaattatttg
     5221 ttttcataca gaagggccag ctgtattagg tccagttgga aagcagattt ctcttttagg
     5281 cgatctagcc gttacttcct ctggtcaaaa caatatcgaa gtgaaccata aaaacgctca
     5341 aaaaggaatt gctgttgccc atgtcgctca cgagcgtggg atttctttag aagaagtcat
     5401 gacgattggc gataacttca atgatgtcag catgctgcaa gtagcaggag tcagtttcgc
     5461 aatgggaaat gcagaaatcg aagtaaaaga ttatgccaaa tatacaacgg acacaaacct
     5521 tgaatctggc gtaggaaaag ccatattgcg cgcgattgca gaagacttgt agttttatct
     5581 agaaaagagg gatatagatg tcagaatttt tcattcagga aaagcagctt ggacgtgcaa
     5641 ctcggacaat tgtcaaaaac gaaaaaggac attccctgtt tttgatggtt ggtcgctggg
     5701 gaacaaaggg cgatgcattg tctctttatg ccatgaatgg cgatctcgtt gctcacatca
     5761 agcagataag cctgacattc ggtacgcgtt ttgagctgta taaagagttc gaaaaggtgg
     5821 gaaccatgca gaaaatattc aattggcccg gtgactttta ttatattcgt agacttcact
     5881 ggacagtcca aggtgatatc tataatcatc aatatcaaat ccatcatttc aatcagcaaa
     5941 tcatgaaaat ggataaagca acactttttt caggagatta ctatgtattg gatgtctcaa
     6001 atgatgaaga tgctccagtt tgtatttgta ttgctgcggt tttagattac tggctctacc
     6061 gtaaagacaa aagtcgcgat aagcctgttt cttaccgact taacttaagt taaaaagatg
     6121 tacctgaaat gcggaaatac gcatttcagg tacatctttt tagatattct tttgcatatg
     6181 atcataaaga tgaccactga tcatccgctg accgacaact ttgaagtcat ggcgttcgta
     6241 taatttttta gcctttggat ttttttcatc cacacttaga ccgatgacgg agcgattagc
     6301 tttcttcgcc aatttaggta atgcttctaa taatcgcgaa ccgatgcctt gtcctctaaa
     6361 atcttctgat acactgatag agtccaaata ccattcgttg ggaaacgttt ctttatcaat
     6421 gaacaaacgt gtagacgcat ctagctgata tttctctaag ataggtacca gtggctgatc
     6481 gatgattgat tcttcttcat ctgtataacc gtaagcaact cctgcaactt taccgtctat
     6541 ttcttctacg atacctcgtg aataactgta acgataattt ggatcagtga ttgcttcttc
     6601 taaaatctct aaagtctttt gttcccctgt ctgcttcaaa aatggcagtt ccatatcttt
     6661 tagaatcact aagatcaatg gggcgatttc tggagcatct tcttttctcg caaaacgaat
     6721 catagtttcc tctccttttc tccattctaa catgcagaaa aagaaatcgc aaaatcgtta
     6781 ttttaaaaaa tcttcaatct tttcagtaat gaaataacca tgagtaacga ttcttgccgc
     6841 ttcagttcgg caataagggc agacgactgg catttcttta tcattatgtt cgactacatt
     6901 attgacttct tcgtatgcaa atttttttcc gcaattaggg catttcattt tttccatgtt
     6961 atctcaactt cctttcatct ttactctctt agcttactct tatattgtaa aaaagagaag
     7021 caatatcctt cttctatgca gatatccata cctgacggtc ctgttctcca tctattaatc
     7081 ggactttata ataaggacgt tcatctttga ctagaagtgt ccactctttc acttttgctt
     7141 ttttcccgta gtagctgact gcttccttca tcgctgcttg cggttgtttg aactcttccg
     7201 ttttttctat atcaaaaaga tgttctgcgg ccttttccgt gtcatttcgt acggttgttt
     7261 tcccagtgga tggattgact gctatgactt gtccttcaca aaaaaactgg tattcatatg
     7321 ttgcattcgt aggattctcc aaatcttttt caactgcaaa atgaatagca gatatgttat
     7381 gttcttttgt ttcgtttttt acgattgctg ctgctttttt caatgagatc cgatattcct
     7441 ggtcactaat atcatccatg tgaaaagcgg cattgagcag tcccgtcata ccggtaatcc
     7501 ctattacgat gagggcagca acaccaaaca tcattttttt cattcttcat tctcctgttt
     7561 ctttttttta aagaatatca agaaatgaac gaatgaaaaa tcgaatgaaa cgaaaaaaaa
     7621 gtgacaattt tgttatcttt attcgtgcag ttctaatggc aatccgtctg gatcaaagaa
     7681 aaacgtcatt ttttttcctg taaaggtatc ttctctaatc ggttctgatt cgatccctaa
     7741 ggactgcaat tctgcaattg tttcttcaat atcgtctact ttgaaagcaa gatgacgcaa
     7801 gccacaagct tctggatagt ttggtcgttg tggcgcatta gcaattccaa aaatctccaa
     7861 ttcagcactt cctagtttca gatctaattt atagtcatct cgttctttgc ggtaattttc
     7921 tctgattact tcaaatccta atttattaac ataaaaatca cgtgatttct gataatctga
     7981 aacaatgatg gctatatgat ggatcgttgt aaggttcata tgagcactcc tattcttcgt
     8041 ttaagctcgg atctatatta atctccagtt ccattgtaaa tgtgtcagat tgaaaaaaat
     8101 agctgttttt cctttttata agaaaaaagg caaaaaaaac agaaagtagg tcctccaaga
     8161 cactcttcct gttcccaata taataaatga aagtaagaaa ttatattttc aagaaaacaa
     8221 acagaaaatt atcataacac aaattattta taattgaaag actagttttt tttcaagaaa
     8281 agcatacaat agattgtgtt agtaaataca gaacacacta catttagtgt cttaaggagt
     8341 cctcatacat gaagtcatat tttcagtttt tagctcatca gctaaaaggc tggccgcaac
     8401 aaaattatta tcttttcttt ttcagtcttg gctgccaggt catgactctt gtcaatagtc
     8461 ccatcacttg gctgtccgtt attacattta tcggtaccac gcttggtgta ttgtgtattt
     8521 tatccatcaa tgcggcaaaa tcagtcaacg gtattttagg tattttgtct gcgatttgtt
     8581 ttattatcgt tgggttttca gcaaagaact atttaagtat cggtgaacag attgcttata
     8641 tgatcacatt ggatatcccc gtattgttaa gtaaagaatg gaatcataat atggcttcaa
     8701 aaatccgaaa attcactgga aaaagctggt ttatcgcaat catcagtact ttccttgtct
     8761 atcttgtttc aggattcctg attggtcgtt atacagatga tcctcggccg tgggtagatg
     8821 ccattgcttt ttctattagc ttgactgctg gtatcatcag tttcttgcgt tacaacaacc
     8881 aatattactg gtggttagct tcagggcttg ctcaaatggt cctttggtat atttcattta
     8941 cgcatggatc tgcaagtttg gcgatgttcg tgaacagctc tgtttacttg ctgaatgatg
     9001 tccttgcatt taccgtttca ccttggtata accaaaaaga acgtgaacgt atgcaaaaaa
     9061 tggaagaaga atactacaac gcacaacaac acgcataaaa aaactgagcg tgggacaaaa
     9121 gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cggcggaatc agaagcaact
     9181 ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct
     9241 tatttcttgg agttaaacgc ttctgtccca gcctctattt tttatagtaa ttccttcaga
     9301 tactttcttg cctcatcatt gaaacgcagt gattcttcta acttctgata atattccaac
     9361 gcttgtttct gagcttcagt aaaacgataa tgttttcctt tccatttgtc gattgtctct
     9421 tgcacatttc cagattggat atctttcact actagttgcc atttttcttt tgagactgta
     9481 cggtctttct tatcagcaga ttgtatagct tctcgttctt tacgcaatac ttttaaaaaa
     9541 ccatttctga ctatccgagg aatgctgtcg aagaaccctt ctgtaaaacc atagcgacaa
     9601 tattgcttcc aaataaatag atagattttt ggttggatga atcgttggtt tttcgcaaaa
     9661 tattccatct gagcagtgac ttctggtgtt cgttgacgtt ttgtctttct caacacggaa
     9721 ataaatgttt caaaataagc attaggatgt actggcactt cttcatatac gatggattgc
     9781 caatctttac gtaaaaatgc gcgatagttg atcaagaatt cttctacatc ctttactaat
     9841 tcttctgcaa agtcgtcaaa tagttctttt tttatctgtc gcttttttga ctgcccattg
     9901 atgtgttcac ttattttctt gatttcattt tccaatgctt gataatccgc tatgtaaatc
     9961 ggcatttcta cttggcgaaa ttctgcgagc cgctgagcta attttatatc cggtaaatat
    10021 ggctgtttca tccgacgatt ttggtacaag acaaaacagt atttttgcca tagtccttca
    10081 ggaaaatcga tattcttctg cttaagccat aataattcgt ctaaggccag atcaactttt
    10141 gtcattccct gatggatcga agctgccact gtcggagaga catgcaggtg atcggcaaag
    10201 tggttgatgc ctagttttag gatctttccg gtttttggcg atttaacgat gtattgcact
    10261 tttaattctc taccacactc acaatacaat ttttgctggc ttcgttttgt tcgaaaaaac
    10321 ggattgatct tgaagtcaga aaacacccaa tctgttgcag ctaaataatg ttcttccata
    10381 aaacgagagc tgattagata tcggcggtgc ttatcgacta gttcccgctg ttttttcgtt
    10441 aattttgcat agatctccca acgttgactg cttgtgtatt gtttagaaat tttgatggga
    10501 tagcttttgc tgatttcttt tggaaaccat tcttcaaaga cggattgcca ttcttccacg
    10561 gctgtttctt tcctttctaa ttacaaaaag ctggctacta acataacgtt agcaaccagc
    10621 cagtgtttct tgctaaatta gtgagttgtt ccggcatttc cggaaatatc ttctttattt
    10681 tctactgctg cttcaactgc ataagtcaaa gaagaagcaa tgtcttctaa agacatagaa
    10741 ggcgtaccca ctggacgatc gatcacttga tcgggtgaga atggcacgtg gatgaaccca
    10801 cctcgaattg ccgggaattg tgtttcgatc aaatgaagca aacgatacat gatatcattg
    10861 cagacaaatg ttcctgctgt ataagagatg tacgctggta gaccatgatc ttgtacattt
    10921 tttgtcattg ctttgattgg caatgaggta aagtaagcgg ctggaccgtt ttcttctaaa
    10981 gccgtatcga atggctgatt tccttcatta tcagggattc ttgcttctgc tagattgatt
    11041 gcgacacgtt cgaacgagac tgctgaccgc ccacctgctt gtccgacaca gatcacgaca
    11101 tctggttggt gctgttggat ggcttctttg actacttgac tgcttttttc gaatacggtc
    11161 ggtacttcca ctttgatgat ttcagcaccg ctgatttcat ctggcatttt ttttactgct
    11221 tcataggctg gattcacttt gtccccacca aatggatcaa aacctgttac tagtactttc
    11281 atctgttgtt cctccgtccg attgatttaa catatggtta tcttttatca taccattttc
    11341 cttgaacagc gtaaataaag gaaacaatag tttaaataac ataacagatc aaacttaata
    11401 tattcttata aaaaaatagt gttccactac aatgttagaa gaggttgcga caattattct
    11461 gtcacaaccc ctctcttcta ttatttaaat agaatcatat aagcaatttg gaaaactagc
    11521 ataaataacg caataaacaa ctggtttttg atcactccgt atttgtcttt catctccagc
    11581 atcgctacag gcacgatatt gaagttagca gccatcggtg tcagcagggt cccacaaaaa
    11641 ccgcaagtca atgcaaccat tcctattaac gctggattcg ctccatgact aaatacgaat
    11701 ggtgcaccga tcccaaccgt catcacggtg atggccgcaa atgcattccc catgattacc
    11761 gtaaatatca ccatgccaag agcgtaaatg atgatgccga tgttcacatt tccttctgga
    11821 acgatcgtac ctactgcatt ggaaatcact gtcccaactc cagcgcttgt aaataccgca
    11881 cctagagaag ctaaaagcat tggcaacatg cttaacggcc ctactgtgcc cagcatatct
    11941 gctgcatcat ctaaaaatgt acttggtcga ttatctcgag aaaagaacat caagattagc
    12001 atggctgcaa aaacaccgac gcccacgccg actaaagcac ctaaattggt aaatagagca
    12061 aacaagatcg caaagacacc gatactcaaa gctggaataa aaattttcat tcctagtttg
    12121 tctgacattt tttccatata ttcttttgat ggaagccggc tttctccttt gctgactttc
    12181 ttagcaattg ctggtatggt catcgcaaag atcaagccac cgttcacgat tgccggaagc
    12241 catcttcctc ctgcaataac tacccctaag accgtccaga aaaaggctga cccataacga
    12301 tgaggattgg ttttatctgt taaatttctt actccagtgt aaatcaatac gagtcccatc
    12361 acgatataaa ggatctccag taatttatcg cctaataaaa tatcaggatt tgtaaaaaag
    12421 tccatcttat tgcccctttc ctggataata tttcttcgct aatttctttt ctttaagata
    12481 gaagtagaca cttgccacaa ctaaagccgt gatagccact ggaacttcca ctaaagcaag
    12541 ctgtcccaat gttacgtcat atcctagatc tttcagtgtt gattggacta gcaacgcccc
    12601 tgctcctccg atgaatagta cttggccaaa gaaccagcag acattttcca tagctgaagc
    12661 catacctttt agttcttctt catgcttagg atggacttct aacccttttg attcgatggt
    12721 ccccaatgcc atcggtaaaa tgattggacg aacaaatcca gcaactccac caaaactcac
    12781 gttaaaagcc gcaaagatca ttcgaaagat cccataaata tctacaatca taccagcaga
    12841 tactttcttg aatcgcttga tcaaggaagc cgctgattct ttcagaccat ttctttcaag
    12901 cgttcctgtt gccaacataa tgatgataaa aatcgccatc ccgcgattgt ttacaaaact
    12961 tgtacctaaa agttctaaca ttccttgaat gccaagaccg ccagtcaaag ctgtaacaac
    13021 taaagcaacc attacgatca atatcgagtc taatttaaat gcaaacccga gaataacaat
    13081 caagacgcct aaaaggctaa gataattttc catatatttt cctccataaa atgacaatat
    13141 ttttttaatt ataaagcaaa acacataaaa aatatagttg ttttctcacg aaatagacac
    13201 tatttttaaa tatttttcgt tattttttta tcacaaaaat tatcttactt attgtaaggt
    13261 aaagcttttt ttaatttatt gcccgataat atatagaaat aaggtctagt tactgttttt
    13321 tttaaatgat taataaataa caacttcttt ctatgaaatt gcaaagcatt catcttcttt
    13381 tccgctaacg atatatccgt cagtgcttgg tacactttac taaaaagtat gagattgccg
    13441 atatctggat aggtgtgcta tactacagaa aaagatatac aatctgccac cgggtaggat
    13501 aaagaggatg atcattccat taggtctttg aagggatgat tttcctcttt ttgtgtattt
    13561 ttttatagtt aaggaggaat gtaagtatgg catggatgga attaatcata gcaggaattt
    13621 tggaagtatt ctggtcaacg atgatgaaat ggagtgatgg tttttctaaa atcaattatt
    13681 ctttttacac agtgattggc atgattgcta gtttttactt tttgtcaaag gcaatcaaaa
    13741 gtttgccgat gagtctctct tatcctatct ggacagggat tggcgcagtt ggttcggtca
    13801 taattggtgt ggtcttgttt catgataagc tgaatctttc cacttggttc tttgtgggga
    13861 tgctactcat cagtatcatt ggcatcaaga ttactagcgg ccactagaca cttggaataa
    13921 gtactgatca gaaaaaataa gcctttttat ttaatattca atttttttat gaaaaaataa
    13981 gaaataagtc caatgaccta gcgacttctc cactcatcag gtgtatgctt gtactatcta
    14041 tagaaaagga gtgaatattt ttgaaaagtc tttcggtcca agaattatcc aagaactttg
    14101 gtcacaaaca agccctcagc gatgtcagtt ttagcttatc ccctggcaaa atcgttggat
    14161 tagtaggtcc taatggcgct ggaaaaacaa cgatcatgaa agctatttta ggactgatcc
    14221 attactcaga tggaacaatc tgtattggtg aaaatgagat ttctccctct agtcataaag
    14281 ggttggaaca agtaggagca ttgatcgaat acccgggtat ctatccattc ttaacaggct
    14341 atgatcatct gaagttattc tctgaaacca acgatgtatc tgctatcgac actatcgtga
    14401 atcagctcaa gatggaaaag tacattcaca aaaaagcgaa atcctactcg ttgggaatga
    14461 aacaaaaatt gggaattgct cttgccttac tgaaccatcc gtctatcgtt attttagatg
    14521 agccgatgaa cggtttggat ccacaagcaa caagagatgt gcgaaaaatc attaccgact
    14581 tggctactca gggcacctct ttcttgatct ccagtcactt actcagcgag cttgaaaaaa
    14641 tggtcgatga gctgatcctg atcaatcaag gaaagatcat caaacaatgt acaatggctg
    14701 aattgaacca aacagcgcaa gatttcatcg ttatctctac aacaaatgat ctagctgctc
    14761 gaacgatctt gacagaagct ggatatactt tggaagatcc tgataaaatc aagcttttaa
    14821 aagaaaacga agaattatta ggaaccgtga tccagctatt gtctgcacaa aatatccatg
    14881 tgacagacgt ccagcatctg acgaatgatt tggaatcttc tctattggaa attctggatt
    14941 cagtaaaggg ggaagaagaa aaatgacatt gcttgtaaaa caagaattat ttaaattgat
    15001 caagaaaaag tcaactgctg ttttatctgt tctattagtt gttttgctca taggaacagc
    15061 acttctagcc aaaaaataca cgacgatcat tgatccagtt gaaatgactg ctcagctttt
    15121 tagtgccaca tcttggattg tctttatcat gattgcagct gcaagcacga tcatttcgat
    15181 ggaagcacaa tatggtacat tgaaaaatct cctttatcga aagtattctc gaggagaaat
    15241 attagtaagc aaatggatca cattagttat ttattctgtt tatttatacc ttttagctat
    15301 tattgtcact gtgttgatga aactgattct tttcccttcg atttccttca cggagaaggt
    15361 ttctactggt caaacactga tccaaagctt gttcacttat actttgggta gttatatcgg
    15421 tttatggctg attttaagtt tagtgttgat gattgcttgc tttatcaata gttccggcgc
    15481 atccatctca gcaggaatcg tcttttattt tgcttcgtca atcatatctg gtatccttat
    15541 tgcactcatc caaaaatggg agtggatcaa atggaatccg atcagtatgt tgaatctgca
    15601 aaaccaaatt ggaaacgaag agatcatgaa gcaattgact catctttcta ctaatcaaat
    15661 gctattcggt aatttagctt acatcgtttt gttcttagca ttaggatatc tcgtatttaa
    15721 gcgaaaaaat gtatgatgac tcatcaaaaa agaggctgtg aaagagacgt tcagccttat
    15781 tgccgattgg ctgtctctta aacaccgttt attctgaaaa aggagctgtg acaacatttg
    15841 tgtcacagct cctttcctag tatgtttttg aataaacgac aggctcacaa cctctcttta
    15901 tacttctttt atacttctat acgtgaagca tcccagtggc aaataaaacc attgcaatca
    15961 ctccgaccat cgtcacacct ataagtaaaa tcttatctaa gtggatatgt tgtacaggct
    16021 cgttttgctc cttacgtgct ttgaggtaaa aataaatgcc tggcaagaaa agaacaagaa
    16081 caagcattac ttcattaaag gctacgacta gcatagccca tacttgaaag gcacttgcta
    16141 aaagccctac taaaaattgg acccattctt tccgctgata agaaagtttc atttggtaca
    16201 atcctaagaa aagccacgtg aagaaaatag ctgctgtacc aagtgtgtaa gcaaagttat
    16261 aagcactgtc agtgaataat aatgaaagta aaaagacatt cgtacataac gtcgtcaata
    16321 cgatagcata agttggtgcc ccatttttgt tgagttttcc ccatgcttta ggcaaaagtc
    16381 cgtcttcagc catgtttccc attgtttcaa tcggtaagat cgtccatgag agccaaacac
    16441 caactaaaga aatgatcaaa ccgccgttga tcacgactgc tccccattta ccaaccatca
    16501 tttcaaacac atagcccata gcaggctgcg ccttgatcgt ttgcagctga ttcatcgtca
    16561 ttgtcccata tggaagcatg gaaaccaaga cgtaaataat aatcaagccg aatatcccta
    16621 gaatcgttgc tttcccaaca tctgattgtt tttttgctcg tgaacctacg acagaggctc
    16681 cttcaatacc aacaaatacc caaagcatca ccatgatgca actcaatact tgactggaaa
    16741 acgggatcgg ctgtccattc gcattcaata taatgtcgct agaaaagtta tcaatgaatt
    16801 gttggatcga aaacgtgctg atagctacta caataaataa aaataatggc actaatttac
    16861 agaatgtgac gatagcattg acgaaactag cattttcgac tccccgattc actagccatg
    16921 catatcccca taaaaagact gtactcatca cgatcgatgc cagattttgc ccaccttcaa
    16981 atactgggaa gaaatatcct aatgcgctca tcaacaatgt tgcaaaagct acgttgccta
    17041 accagcatgt gagccagtaa ccccaaccgc tgataaaacc agcgaaacgg ccaaaacctt
    17101 cttctgcgta actaaagatc ccgcctttta aatctggacg ttttttattg atattattga
    17161 tcgacaagga gagcatcaac attgctgttc ctacgagtac ccaactgaaa atagctgggc
    17221 cgccagccga tccggagatg tccgtcataa taccaaatac gccacctcca atcgctgaac
    17281 caattactaa ggcagcaagt ggaattaaac caattccttt tttctgttgc tctgtatcca
    17341 tgtaagcatc ctccaaaata aaaccgcttt tatcaaaaag tataacaaac cttttttatt
    17401 tttacttaat taataaaaac tttaatagaa atataattct accggctttt ttctcattat
    17461 acaagcaaag cgcctagtcc ctttccaaat tacaccacca aaaaacttaa aaaagatgct
    17521 atactaaaag gaaagaattg gaggatgatc catgaacatt ttatatatat cgatttcagg
    17581 aaatacgcgt gcctttgcca aacatctagc agaatatgct gaaaaaatgc atgcagaaga
    17641 tcctgtcaat cctgaagtta ctttaaaaga aatccatgaa aatagtgatt ttacgaaaga
    17701 aacagaacca ttttttactt tcgttcctac ttatttagac ggcggaaatg gtttagataa
    17761 cggtgataca gaaatcttga ccgaaacgat gcgtgaatat ctggaacatg aagacaatca
    17821 taaactttgt cttggtgtag tcggtagcgg aaataaaaac ttcaataatc agtattgtct
    17881 aacagccaaa caatatgcac aaacatttgg ctttcctttc ttggcagatt atgaattacg
    17941 gggaacacca tctgatgtga cacgaattta tcagatttta gcagaaaaca ataaataata
    18001 aaaaagaaga agctgaaaaa tagatttcag tttcttcttt cgtttctatt gtttctatca
    18061 tattaagctt tgacatccat cacaggtgct tttgcttttg ctttacctgt tttttccaag
    18121 acaaagtttt ttacacgatc catattggtt accaagacga tcatggatgt ttctttgcct
    18181 gctgctttga tcgcttctaa gtcagcttct gctacaagcg tatcaggtgt cacttgctgc
    18241 ccttctgtta ctttcacatc aaatggcgta ccgcctaagt caactgtgtt gattcccata
    18301 tgaagcaaga tttctaaacc atttggcgtt gtgatcccaa tagcgtgttt cgttgggaag
    18361 acagagctga ctaccccagt aactggagaa tagatatgtc cgttttctgg ttctacaaag
    18421 tagccttcac ccatcatctt ttgtgcaaaa actggatcac ttgctttatc taaagaaaca
    18481 actgttccgt cagcaactga tgagaggata gtcacacttt cagctgaagt ttgaggtact
    18541 actttttcag gcacttcatt tactgtggct gtttcagtcg ctaattcttc tttttctaaa
    18601 cggtctttag gaataccgaa gaagaatgta gcaacgaagc caccagcata agctgccaat
    18661 aatccaagga tgtagcctaa ccatttaccg tcagagatca atgggatcaa agcggctcct
    18721 gaaggtccaa tagcgatagc cccgacattt ccgatcattc cgatcactgc cccgccgata
    18781 cctccaccga tacaagcagt aataaatggg cgtcctagag gtaatgttac tccgtaaatc
    18841 aatggttcgc cgattcctag gatcccgact ggtaaagctc ctttgatcat ttctgttaat
    18901 tctttatctt ttttgcagcg aatccataat gccaatgctg cacccacttg tcctgcacca
    18961 gccatcgcaa gaattggaag caatagcgta cgaccagttt catcaatcat ttgcacatgg
    19021 attggagtca aaatttgatg cagtccgaac ataaccatcg gcaagaataa tgtgcctaga
    19081 acgaagccgg caaatgctcc accaacgcca agtacccagt tgatcccacc gaccatgcca
    19141 tcagaaatga aacctgctaa aggcatgatg aagaaaattt caatcagacc gatggcaatc
    19201 aatgaaagag ttggtgtaac gatgatatct accgcatcag gtactacttt atgcagtttt
    19261 ttctcaacga ttgacaatag ccaaacagcg aagatgacac ctaggatccc accttgtcca
    19321 gctgcaagag cttcaccagt aaataagttt ttcagtggtg cttccggatt catccctgtc
    19381 agcatcacaa cggccccgat aactccacca agagttggtg ttgcaccaaa aacttgtgca
    19441 gcattgatac ctgtatagat aactaggtag ctaaacatcc cgtttttcaa gatgttcatc
    19501 acatcgatat attgttgcca tgttgctgca tcaagtgttc cggctgtgac taagtttgct
    19561 aaaactgcgg caatccctgc cacgatccca gtaccgacaa atgcaggaat cattggcaca
    19621 aagatattag aaatgtcttt taataccgat ttgattttag atggtttttg tttcgatttt
    19681 tgtgccgctt tcatttcttg tgcccgttcg tttacacggt cttttccaga tgctgatgaa
    19741 tttgctgtag ctgtacttac agaagaaagt ttttctccta atttcacgcc tgccaaatca
    19801 accatttgct gtgctacttt gttcactttc ccaggtccga tgatgacttg aagctgttca
    19861 tcttctacta caccaagcac tcctggaatc gcttttaatt cagagacatt tacaagatca
    19921 tcattgatga tcttcatacg cacacgagtc atgcagtgga caatgctttc tacattgccc
    19981 atgccgcctg cccctttata aatttgtttt gccagttctt ccaaagtcag ttcgtttttt
    20041 gccatggtct tttccccctc ataaggtccc ttttacaaat ccgtttgctt cagctaattt
    20101 tgcttgagct tcctctttat cactatttgt caaaatcatg acgatagcca gcttaacatt
    20161 ttgatctgct tcttcaaagt acttttcagc tgtttcgtaa tcagcttctg tcgcttgcat
    20221 gatgattcgt tttgagcgtt cgattagttt ttcatttgtt ggtttgacat cgaccatcaa
    20281 gttattgaat actttaccta taccaatcat actgatagta gaaatcatat tcaagataag
    20341 tttttgtgct gttcctgact tcaatcgagt agagcctgtc aaaaattctg gacctgcatc
    20401 gacttcgatt gggaaatccg cgtgtttact gatttcggct tgtttattgc aagaaatcgt
    20461 tcctgtgaca gcaccgatcg attttgcgta atctaatcca ccgatcacat atggtgtacg
    20521 accgctagct gcgataccaa taactatatc attttttgtc aaattcagct tgattagatc
    20581 ttcttgccct agctcataag aatcttcggc cccttcgaca gctacagtca ttgcttcttc
    20641 tccaccagca attagaccta tgaccatttc cggttctaca ccgaatgtcg gtacgcattc
    20701 agcagcatct aacacaccta gtcttccact tgttccggct cccatataaa tcagacgtcc
    20761 acctttttta aaggactcaa tcgtgttagc aatcactggt tcgatacttg tcagttcttt
    20821 ttcaaccgca gcggccactt tctgatcttc tttgttcatt agttgaacgg cttgagaaat
    20881 actcatctca tctaatccaa aagtttcttg gtttctgcgt tccgttgtta ggttctctaa
    20941 attgattttg ctcattttta tcttccttct tttcttataa attgatagtt cataccagct
    21001 ttgatttggt tgatcaatgg tagctcttct ttgataactt tagctactac attcactttt
    21061 tcatccgctg gcaaatcata tttggttaat tggatctcac ccatatagcg caaatacttt
    21121 tcgttatcta aagtaataga ccctttcata cgcgtagctg tatttcttgc tgggataggc
    21181 ggaatctctc gaaaacgtgc atctacgctt cgtatgacat ctcttgcatc gtcttgccga
    21241 ttcgtatgct tccctaatat atattcgtaa aattcttcat ctacaggttc cgtatggagc
    21301 aagactgtct gttctttttg gaaagaagca aattgctcct gaacgttttc tgacagacct
    21361 gcatctccga tgtaaacaat atccgtgtta cttgctaata gatctagagc gcctgcaaga
    21421 ggatggatcc ctcgatgttc ttccaaagtt ggcaatcctt gatatagtgg gcctctcaac
    21481 tctgtatctc ctggtacaaa accttggaca aggagacctt gaattttgag ccattgattt
    21541 tttttctgat accattcttt gtccagccct gtttcgggac gtggataata attatgccaa
    21601 gcttcaatct gtgagaaatc agcttctgct tctttcaatt catcgatatc tttaggcgtg
    21661 atcgtgctag cattcaaact gattttcaat tgatgtgacc agtcggcaat ctgctgattg
    21721 gaaatatgat aatccattct taaacctgtt acaccaattg ctttcaaagg cgttagatct
    21781 tctaatgaaa aacccgctcg ttccaatgcc tctcctgaaa tatcgaccat caattctagt
    21841 ttattctttt tcgcttcgct tcctagttcg atcaatcgct tttgatatgc cgaaacatca
    21901 tcttctggta tatgcatgga agtgaaaata ccgacaaagc cattggatgc catttggcgg
    21961 atatactgtt tcttctcttc tgacaaagaa tcgttcataa atattgaaaa gccaaacata
    22021 ttatccctct ttttctgtat ccgtcttcat ttcta
//
LOCUS       DAMYXM010000045        21820 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.046, whole genome shotgun sequence.
ACCESSION   DAMYXM010000045 DAMYXM010000000
VERSION     DAMYXM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.046"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..422)
                     /locus_tag="PNS21_001845"
     CDS             complement(<1..422)
                     /locus_tag="PNS21_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HDI5159553.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYL"
     gene            complement(509..1213)
                     /locus_tag="PNS21_001846"
     CDS             complement(509..1213)
                     /locus_tag="PNS21_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HDI5159554.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            1413..1679
                     /locus_tag="PNS21_001847"
     CDS             1413..1679
                     /locus_tag="PNS21_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI5159555.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            1692..1850
                     /locus_tag="PNS21_001848"
     CDS             1692..1850
                     /locus_tag="PNS21_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159556.1"
                     /translation="MEEKNIKVPTLEEYTKIVVETDEQNPKTIAVITANDIESCEGFR
                     VRMTPRYD"
     gene            complement(1847..2077)
                     /locus_tag="PNS21_001849"
     CDS             complement(1847..2077)
                     /locus_tag="PNS21_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDI5159557.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            2149..2304
                     /locus_tag="PNS21_001850"
     CDS             2149..2304
                     /locus_tag="PNS21_001850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159558.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            2461..2616
                     /locus_tag="PNS21_001851"
     CDS             2461..2616
                     /locus_tag="PNS21_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159559.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGEKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2613..2837)
                     /locus_tag="PNS21_001852"
     CDS             complement(2613..2837)
                     /locus_tag="PNS21_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDI5159560.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            3104..3439
                     /locus_tag="PNS21_001853"
     CDS             3104..3439
                     /locus_tag="PNS21_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159561.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3672..4613
                     /locus_tag="PNS21_001854"
     CDS             3672..4613
                     /locus_tag="PNS21_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDI5159562.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            4615..5505
                     /locus_tag="PNS21_001855"
     CDS             4615..5505
                     /locus_tag="PNS21_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDI5159563.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5548..6348
                     /locus_tag="PNS21_001856"
     CDS             5548..6348
                     /locus_tag="PNS21_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDI5159564.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6363..7214
                     /locus_tag="PNS21_001857"
     CDS             6363..7214
                     /locus_tag="PNS21_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDI5159565.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7211..7528
                     /locus_tag="PNS21_001858"
     CDS             7211..7528
                     /locus_tag="PNS21_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159566.1"
                     /translation="MNLTKQEKRICRQIMLQIIGDRDMDELSQQESRMVRKISKLIGA
                     DTMDRSKPLPKLNFESFTFEKYSHLREVGYSVNAIRKALGVGSSVWAKWRKENVNEVN
                     QND"
     gene            7521..7703
                     /locus_tag="PNS21_001859"
     CDS             7521..7703
                     /locus_tag="PNS21_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159567.1"
                     /translation="MIKLIGKETLKVYYVAETKAEVSRWLLKMFNKTRANASVSQKAS
                     VSETGMPEAMWISRRN"
     gene            7735..7824
                     /locus_tag="PNS21_001860"
                     /pseudo
     CDS             7735..7824
                     /locus_tag="PNS21_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            7899..8366
                     /locus_tag="PNS21_001861"
     CDS             7899..8366
                     /locus_tag="PNS21_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HDI5159568.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            8894..8966
                     /locus_tag="PNS21_001862"
     tRNA            8894..8966
                     /locus_tag="PNS21_001862"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8927..8929,aa:Lys,seq:ttt)
     gene            9352..9612
                     /locus_tag="PNS21_001863"
     CDS             9352..9612
                     /locus_tag="PNS21_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159569.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSVFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            9655..9828
                     /locus_tag="PNS21_001864"
     CDS             9655..9828
                     /locus_tag="PNS21_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159570.1"
                     /translation="MKQYTAKDFEEMKRLKKDYEEVDMELTVGVIQRRLRVGLETAKA
                     IYNDLNAIEEKNG"
     gene            10217..11050
                     /locus_tag="PNS21_001865"
     CDS             10217..11050
                     /locus_tag="PNS21_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HDI5159571.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFETLSDEEKDIYSSLNDNPSFVLSEEIRLLKIRQFRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKNPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11043..12458
                     /locus_tag="PNS21_001866"
     CDS             11043..12458
                     /locus_tag="PNS21_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HDI5159572.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            12470..13999
                     /locus_tag="PNS21_001867"
     CDS             12470..13999
                     /locus_tag="PNS21_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDI5159573.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            13917..14969
                     /locus_tag="PNS21_001868"
     CDS             13917..14969
                     /locus_tag="PNS21_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDI5159574.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            14971..15288
                     /locus_tag="PNS21_001869"
     CDS             14971..15288
                     /locus_tag="PNS21_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159575.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            15415..16038
                     /locus_tag="PNS21_001870"
     CDS             15415..16038
                     /locus_tag="PNS21_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HDI5159576.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16053..16943
                     /locus_tag="PNS21_001871"
     CDS             16053..16943
                     /locus_tag="PNS21_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HDI5159577.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLFGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            16966..17181
                     /locus_tag="PNS21_001872"
     CDS             16966..17181
                     /locus_tag="PNS21_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159578.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            17193..17534
                     /locus_tag="PNS21_001873"
     CDS             17193..17534
                     /locus_tag="PNS21_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159579.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            17534..17905
                     /locus_tag="PNS21_001874"
     CDS             17534..17905
                     /locus_tag="PNS21_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159580.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            17898..18293
                     /locus_tag="PNS21_001875"
     CDS             17898..18293
                     /locus_tag="PNS21_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDI5159581.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            18295..18672
                     /locus_tag="PNS21_001876"
     CDS             18295..18672
                     /locus_tag="PNS21_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159582.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            18673..19281
                     /locus_tag="PNS21_001877"
     CDS             18673..19281
                     /locus_tag="PNS21_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI5159583.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            19281..19622
                     /locus_tag="PNS21_001878"
     CDS             19281..19622
                     /locus_tag="PNS21_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159584.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            19864..>21820
                     /locus_tag="PNS21_001879"
     CDS             19864..>21820
                     /locus_tag="PNS21_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDI5159585.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKY"
BASE COUNT         7812 a         3249 c         4490 g         6269 t
ORIGIN      
        1 ttcaaataag aaggaatatt aattgcaccg tctaaaataa aatccttttc tttaatcttt
       61 aataaagaaa gtttgttttt tatttcggat gaagaagttt catctatatt cggaataatt
      121 actgatttat agatagtgac cagagatgtg tcatattttt gattaatttc atcgtctaac
      181 cgctggcttt cagcattaag aatatcaatt ttgttttcta gcgttgacaa gtttttattc
      241 aatttactta tgtttttctc tttttcagat ataaacgttt ccctagcatt taattcttga
      301 gaatattcct ttaccttact ctctaaggca gaagttttat cttttaattt tttagcttca
      361 gataatttgc tactacccat aattattaaa actattccaa ctaaaaacaa tagtactccc
      421 attcaaaacg cctcgcttct tttataaaat aattgtcaaa atcatataaa cccttaccga
      481 agttgacggc tccggcatgg gaattttttt aaaatacaac ttttccgata attctaatct
      541 gttctggcga tacaattcta tcttcgtata agtcgtttat agaacgtaaa atgattgttt
      601 gattttcgta atctttatat atctctttac atgtaactcc gtcaccacct aattccacaa
      661 tagcaatttc cccatcttct acgtccggtt gttctttaac gaatacgaat tgtccattcc
      721 tgataacagg ttccattgaa tcgcctataa taggtactgc gaagtcagca gcacttggaa
      781 ctctctcaaa ttcttcctct tcgacaaccg tatcaccgta agtaagttct gttggattgg
      841 cagccgaagc cgaattgtga acagttggga ttttttcttt tttaggaaaa tctaggacat
      901 tttcttgttt ttgttcttct aatcgattac ttgcaaattc ataggtagca actttatttt
      961 cgtcgttaag ctgattatat ataggaagga tattatatat ctgattagat atagataaat
     1021 caatattcat caaatcatct atatcaacat tgaatatctt agctatctca tttaaggttt
     1081 ttaattttgg tgtatatttt cctttttccc attcgcttat tgaggatgaa ctttttcttc
     1141 ctagtttttc ggctagttct tgttgatcaa tattatactt ttctctaaga aactttagat
     1201 tttcagcgaa catagtaaca cctccttgag ttaattgtaa caccgtttcg gaaaaaatgt
     1261 aacaggtttc ttagaaaata ttttagtgtt tttaaagaag tatttacgca aaatagaaat
     1321 cgatttcagg aaaactgaaa tatgcacttg acttcggaaa aaccgaagta tataatttag
     1381 tcagaaagtt aaagagagga ggcgaataaa atatggatac cgcgaagaaa gaaaaatata
     1441 cattgagaca attaagagct ttaaagggtt attcaaaaga agaactgtca aggaaaagca
     1501 aagtgacatc aagaacaata tttatttacg aaaacgatgt agataaaatg aggaacggaa
     1561 agtacgccac tctagaaaaa attgctaagg cattgggtgt tagagtttcc gatatttttt
     1621 tagaccctga ttcggaaaaa ccgaaatcaa taacaggtga aacaaaacaa gtcagctagg
     1681 aggtgtgaat agtggaagag aagaatataa aagtaccgac tcttgaagaa tatacaaaaa
     1741 ttgttgtcga aacagacgag cagaacccga aaacaatcgc agttattacg gcgaatgata
     1801 ttgaatcatg cgaaggtttt cgagttcgaa tgacccctag atatgattaa tgttctttat
     1861 cacgtggagg tataggatcg tttccaaaac tatcttttga ttgtatttgt ccattctttt
     1921 tttggacaat aaactctgat tgctgttttt tagaaatctt tttaccatac tcaacagcat
     1981 ctgatttatt atcaaatagt tttgtatctt cggaattacc ttcaccacgt acagcccatt
     2041 tgccatttct aggagatacc cattgatttt tacccataac aattcacctc gctttcaagt
     2101 taattctatt aagaggtgaa aagaaaacaa actgatggag gtgagattgt gagtaacaag
     2161 ccaaaaatca ctttgaatgg cacacctaat cttcaaagat tagcagatga aatggctaga
     2221 atacgttcta gacattgggg agttactgtg gtggcaacca taattgaaca aaaagaaaag
     2281 cccacacagc cggcaagcaa gtagtgggca ccatagaaca ataatattat atcacacact
     2341 cgccacttcc gaaaagagga gttgctgctc tcctttcaag tttgccaaag tccacgtttc
     2401 ttcattttgg caactcctct tcttggaggt ggtgacggcg agagacgaag gagggatgac
     2461 atgaaggaag aatcaaaaat agggattttg aatgagaaca attacacaag attaattatt
     2521 gaaacagaag aaggggaaaa agtagcagaa gttacattga ccgatgcaac tcctgctgac
     2581 ggttatcgta ttcgattaac tcctaaatat gattaacctt ttggtgggtg tggatcgttt
     2641 ccgtgactat cacggctttg aatttttcca tctctgccat gaataaatag ctcagatcct
     2701 tgatttttag aaatctctct agcgattttt actgcttctg attttgtatt agtatgtgca
     2761 gtcgctttag aatttccagc acctttgaca ttccaaccac cattggaatt aggtacaaca
     2821 tgttgatttt ttcccatagt tttcacctcc atttctatag aaatatttta tcaaatagaa
     2881 atggagaaat tcaataaacg aaaggaggga tcaaatgaca ttacaagagc atcaacactt
     2941 gatgcgaaaa ctaaatcagg aataccacga ggaaatgaaa cctagattgc taggggaaca
     3001 atggcgtgaa cgccagaaga agtggttaga aataaaaaag tgactcagcc gtccaaagca
     3061 atgagtcaca aaccaaaaat tatacctaag gagattttat cacatgaata agaaaattga
     3121 aaatcttatc gaagaattga aacgtgaatg ccaaaagcaa ggtgtttcta ttatttgtac
     3181 agcccaaaaa gaaggggaac ttaaaagtct cgtttatggt gagacaacag aaattttact
     3241 ttgtcttgct atgcaagaag aacatttaga tgaaaatctc ccattgtcag cacatattat
     3301 gcgtagaatt gctgtggatg cttacgaaca agcgaaaaat gaagaagaaa atcaaccttc
     3361 aaatcatact tttgttatta ataacaagga agatttagct gatgtgatga ctcgtattct
     3421 caagggggaa tttcaatgat gaatgaaaaa acaattgatc tactcattat tttatttttc
     3481 acgttagtga ttgtagcgaa aagttatgta atctttggct tgttattcgc aattaccatg
     3541 cttgtgcttt gcgtgttagc aagcagaaaa gaggttaaac atgaacgagt tagcaaatct
     3601 tgacaactat ttaactgatc ctgattatgc taaaccacca tatgaagcgc caattgatga
     3661 ggaggatgaa gatgagtaaa tctaccttag aaatgagcca tcaagaatgg ctcgaagacc
     3721 gcaaaagagg tattggtggc tcagatgttg caaccgtact tggattaaac aaatacaaat
     3781 ctccttatca attgtggctt gagaaaacgg gtcaaattga attgaaagat ttagaaagcg
     3841 aaccagctta ttggggtaat gttttagaag aagtggttgc taaagagttt caggaacgta
     3901 caggcaaaaa agtacgcaga agaaaccaag tatttgaaca tccattacat ccatttttaa
     3961 gagcaaatat tgatcgggaa gtagtgggag aaaatgccat tctggaatgt aaaacagcca
     4021 atcaatttct cggaaaagag tgggaaggtg aagaggtacc gcttagctat ctctgtcaag
     4081 ttcagcatta catgaacgtt ttaaacaaag actattgtta tatcgctgtt ttgatcggtg
     4141 gacaaaaatt catttggaag cggattgaac gagatcaaga gctgatcgat acaatcactg
     4201 aacaattagt agagttttgg gaaacgaacg ttcttggagg tatcgagcct gtaattgacg
     4261 gaagtggagc gactgctgac ttcttaaaag aaaaatatgc agatgtagaa gaaaatcaaa
     4321 cagctctacc atcacgtttt gatgaactta tcgagcaaaa aagagaactc aagcggacga
     4381 aaaaagaaat tgaatcatct attcgtcaag tagacaacga gatcatcagt gagctaggta
     4441 aacgtgaagc cagtatcggt atcacacaaa gaaacattat cagctggaaa cttgttcgta
     4501 caagacggat gaacacgaag aaactagcag agaaatatcc agatgtcgca aatgatgaag
     4561 agatttataa cgttactgaa tcaagaaggc taaccgaaaa ggagatcaaa taatatggca
     4621 acaaatgaat cgttaaaaaa tcaattggca gaaaagccac agaaacaagt tgcaccagga
     4681 cagttagggc ttaaagctct aatgaataca ccaacaatga gaaagaaatt tgaagaagta
     4741 cttcatgaca atgctaatgc ttttatgtcg aatgttatga ctcttgtatc taatgacagt
     4801 tatcttgcag atagtgaacc gatgtctatc atgagtggtg cgttaactgc tgcaacatta
     4861 aatcttgggc tagataagaa tttaggttat gcatatttag ttccattcaa tagtaaaaac
     4921 aagcaaacag gaaaatggga aaagaaagct caattcatgc ttggctataa aggatatatc
     4981 caattagccc aacgatcagg taaatacaaa gcattaaatg tgatcgaagt ttacgaagga
     5041 gaactaaaaa gctggaaccg actgacagaa gagtttgagt ttgatccaaa tggtagaacg
     5101 tctgatgaag tcattggata tgttggctat ttcgagttac tgaatggatt caaaaaaact
     5161 gtctattgga ccaaacaaga aatcgaagct catcggattg ctaacaataa agatcgcgat
     5221 aagacaaagt taagtggtgt gtgggcatct gattacaatg caatggcacg aaaaactgtt
     5281 ttgagaaatc ttctttctaa atggggaatc ttgtccatcg aaatgcaaga agccaccaca
     5341 tcggatgaga gagtccaaag ggttcaagaa gacggcagca ttattgctga aacagaagtt
     5401 gaagaagata ttcctgaaag aaaagaagca gaggttatat ctgaagaaaa tgaagatgta
     5461 caaactggat tatttgatgc atctaatccg ccgttaaaca aataatgagg gagctttctc
     5521 ccttgctttt ctagaggaga aatacgaatg aacacaggat atgtcaaatt acatcgaaaa
     5581 gtgatgaatt cattcgtttg gaccaatcct tatatgtata aattatggag cttgtgtttg
     5641 atgaaagcga gtcatgagaa tcgcaagata ctttttaatg gaaaagagat acaagtgaac
     5701 agcggagaat tcgtcacagg gcgcgatgct atcacatctg agatgaacga aggcgtgaaa
     5761 cgtgaacatc aagtgaacag cggttctgta tggagatggc taaaacaatt tgaaaaaaac
     5821 ggaatgttga acatcaaatc aactacgaaa tacagcgtta tatcaataaa aaactggtct
     5881 ttgtaccaaa gcagtgaaca acaaatgaac atcaaacgca caacaagtga acaacaagtg
     5941 cacacaaaca agaatgaaaa gaattataag aatgaaaaga ataataaaac atcatcgtcg
     6001 caaccacgca aaaagcgtgt ttacgacacc gactcagttt actacattct cgcggaggag
     6061 ttattcaaac agatttgtca gaatcaggaa atcaaaaagc cgaatctgca aagttgggca
     6121 gataacattc ggaaaatgat cgagatcgac aagcggaccg aaaaccaggt acggggaatg
     6181 attgaatgga gtcagcacaa tgtgttttgg gcatcgaata ttttatctgc taaaaaattg
     6241 cgagaaaaat acgacacaat ggcagcgcaa gcaaatcgtg attataaaac aaaacaaact
     6301 aaaacgcttg aatacgagaa atttagcaca gatgagttgc ctatttgaga ggaggcgtag
     6361 gcatggaaac tgttggggaa atcatggaaa agctgataca gaaagtgctt gtccaacgtg
     6421 gcgaatgtcc tgaatgcgga cagcctttgt atggatggcg tacgaagaac cctgatggct
     6481 cagaacgttg taagccaacc tgtatgcaat gtgggtataa agcattgcga gttcaggaag
     6541 acttacaaac agaacgaatt tacaacgaga gcctgaaagc aagagcaatc aattttttca
     6601 aaggtggttc tgttgtgcct aatcaagcgt tgtttgattg cacattgcag aattatcaaa
     6661 ttgtcgatca agaaacaaga caagcggttg aagtaaccaa acgctttgtt aattcagtct
     6721 tgttaggaaa tccaagtcac cttgttttaa ctggaaaaca aggaacaggt aaaagtcacc
     6781 tagcgatggc agcggcttgg gaagtcttga agcgatcaaa ctacgataaa aaaatcttat
     6841 ttatcgggtt acaggaaatg ctggatcaaa tcaaattttc gtacaacaat cctgaactca
     6901 gaaaaacgat tgagggatcg ttgattgcag atatcaaaac agcggattta gtcatcattg
     6961 atgatatcgg ttcagaacta ggaaaagatg catcagatag tcgagcgttt ggcataaaca
     7021 cgctaaattc gttcttggat gcacgacaga acctagcaac gattatcaca acgaacttgc
     7081 ttggtgaaga actgaaaaaa gcttacggta cgagaacaat atcaagaatg tttgtcaact
     7141 ctgatggatt tacgatggta ttttctcaaa cagcagacaa gcgcataaaa ccagtcaaag
     7201 gagcgatcgc atgaatttga ctaaacaaga aaaaagaatc tgtcgtcaga taatgttgca
     7261 aattataggt gatcgtgaca tggacgaatt atctcaacaa gagagtcgaa tggttcggaa
     7321 gataagtaag ctaatcggtg cagatacgat ggatcgatcc aagccattac ccaaactgaa
     7381 ttttgaatca tttacgtttg agaaatatag ccatctgaga gaggtaggct atagtgtaaa
     7441 tgctattcga aaagcacttg gggtaggaag tagtgtatgg gcaaaatggc ggaaagaaaa
     7501 tgtaaatgag gtaaatcaaa atgattaagc taataggcaa agagacgctc aaggtttact
     7561 atgtagcgga aacaaaggca gaagtcagcc gttggctatt gaaaatgttt aataagacta
     7621 gagccaatgc ttcagtaagt cagaaagcaa gtgtatcaga aacaggcatg cctgaagcaa
     7681 tgtggatttc aaggaggaac tgagatggca atgataccga agtttagagc gtgggataga
     7741 gaaaaagaca gaatgaaggc agtagctttg gttgaatttg ctgaggcgta taaatcagga
     7801 gaactcattt cacgtacaaa ataaaaaaag tcggattcct ccgactatga ataatatttc
     7861 cgacataagt attatatcat aattggggga atcagaggat ggtacttttc gatgtaaaga
     7921 aatatgagac accaagcgcg aaagatgttg atatggaacg cacaaaacat aatgtcgctg
     7981 tgtttctttc agcatatcta tcagctagat gtagagtagg gcaacctcga gaaccaaaag
     8041 tgacagcatc ttattccttg gttccacctt ctacagctga tcatatattt gaagcagaaa
     8101 gaatgctgat cgataaagaa gaagcacagg aagaatttga gtatctgcat aaattgtttg
     8161 ttcgaggcta ttcagcgata cagcatccgc ataagcctga tgtgactgaa aggcgcaaga
     8221 agatattcta tgatcgttat atcaatggtc tgcccattta tgtaactgct caaaggaata
     8281 atactagcga agaatcggtt aaagtagaat caaacagaat tatcatccaa tttgcttcat
     8341 cgttagaact ggttgctttc aagtagccag tttttacact ttttataccc ttttattacc
     8401 aatttggttt ccattttata ccttttttat accaatcact tacctattca acgttgtatt
     8461 atgatagtgt cgaaagatta ggaaacagga tcgacaaaat aaaatgtaag ggaggaaatc
     8521 tccctcatcg tttaattaag cttcgatagg cagcagcgga aatattaaga ataaggatgt
     8581 gaattttaac tccttctaaa ttgttcttat tatctatcat ccgttgctgt ctattgttat
     8641 tatgtcactg tggcggaaag ggtagacgct taaaaataag gtcaatacgt cgagggatag
     8701 ccttaacgtt ttatgatttg accatgcaag gttcgattcc ttgccagcga cttagacgaa
     8761 aggtagctta aatattaacc acatcaacat cgtcagcgag ttgataaagg taactagcag
     8821 actctaggat ttaagccagt cttaccgatt agtctgttat agtagcaagc ttgctagagg
     8881 tagctcctct ggttgacgtg cagctcagtt ggtagagcgc ctgactttta atcaggaggt
     8941 cgccggttcg aatccggtca cgttaataaa gaacctacgg aaacaatcaa tcttatcgga
     9001 tgccgattgg ttggctgaat taagcagtgt gtctgtgaat cagttaatag gcataaaata
     9061 ctagcacaga cgtgtgccac tctcaggtgt aggttaggag agaaacatta gcaaccgagg
     9121 gttggaaatg ggcgctcaaa gtacacgagc aaggcgaggt cgatagtaat cgatggaatc
     9181 ggtgtaggtt gctattacat aattgattgg gttagattga gttttgggat tcggtacaaa
     9241 tgaatcgtca aatgactcaa gcacaggatc ggaaacgtcc ctgcctgtgc attacatatt
     9301 agatcactcg ttgagtggtc tttttatttt agaaaagaga ggattttgaa aatgaatcat
     9361 gagaagttta tcgaaaaatg caaggctatt gtacgtgaaa gaattgagaa tgagattgct
     9421 gacctaagtg gagcagtacc tgagtttagt gttttcatag tctggtcatg caaaacacta
     9481 cagaatagca aagcattagt cagcgctagc ttaaaaggag caccgtattt tgaaattacg
     9541 ttgaacgggg acaaaggtga aatctatgta gatacttatc tcaaaaaatc aaatgaatgt
     9601 atcaaagtct agcaggtgct aggctttttc tttacataaa ggaggaatga tctcatgaaa
     9661 caatataccg ctaaagattt cgaggaaatg aagcgattaa agaaggacta tgaagaagtt
     9721 gatatggagc taactgttgg agtcattcaa cgaagactgc gggtcggatt agagacagca
     9781 aaggctattt acaatgatct aaatgctatt gaagagaaga atggctaatg aggaactact
     9841 ggtatatatc gctaactaat gaatatcctc gaaccattga tgattgttca gtgcgtgttg
     9901 tgcgttctgt acaaatcaaa gggaagtact ctattgtcga aatggggaga gaagcagaac
     9961 catgtgagat tgatgcgtgc aagctcgttt attgcggtca tggtttctat gatgaaccaa
    10021 acattcaaaa taatattaac aagaatttga gggattagaa tgcaagaaat ggcttaaaaa
    10081 tctccaatca ggacaaatca agttagacaa agtttccgat ttaaagatat taattgaagc
    10141 agatctaatg ttgaaagata ttgaaaatta gaaaacaaaa ctcaacctaa gaagattgcg
    10201 aggtggtgtg tattgaatgg caagacaacg tgatccaaga cgtgatgaag ccaaaagaat
    10261 ttggttagaa tccaacggag aaaagcagtt aaaggaaatt gcatctgaat taaatgtttc
    10321 agattctcag gttagaaaat ggaaatcgca agacaaatgg agcgctgaat tgaaaagtaa
    10381 cgttaccaat ggcaaaagta acgttactaa tcaaggtggc gctcctattg gtaatcaaaa
    10441 tgctaaaggt aacaaaggaa atagccgagc ttctccgcca gtgggtaata aaaacgcttt
    10501 gaaaacaggc gagtatgaaa ccatattttt tgagacacta agtgatgaag agaaggacat
    10561 ctattctagt ctgaatgata atccttcttt tgttttgtct gaagaaatac gtctacttaa
    10621 gataagacaa tttcgtatga tgaagagaat ccaacaagct gaagctggac taaatgatga
    10681 agaagtcgaa cgattgcagc agctaagaaa gattaaaaat ccgattgaaa aaaatggtaa
    10741 aaagctagaa atcaagcgtg aggttatgca agatgtgcag attagcagaa aaaaacatcg
    10801 caaaattgat gatattcttt caattgaaga ttcattgact cggattagca accagttagc
    10861 taaagccatc aagcaaatga atgaacttta tatgaatgaa tacagaactg atttaattaa
    10921 agctcagact gataagatcc aagctgagac aaatgaaatt ggcggaaata attcaggtga
    10981 agaaatagaa gaatggaaac aggcagtttt aaatgccgca aacaaacggg cggtgaaaga
    11041 aaatgaataa tgaatttatt ccttttgctg atattggttc tgccattgat tattactatg
    11101 ataaaccagt agctttttgc caagatattt tgcatttgaa tcctgatgaa tggcaagaaa
    11161 atgttttaaa tgatttagct gaattttcaa aggtttctgt tcgttctggt caaggagttg
    11221 gaaaaacagc attagaagca ggagcaatac tttggttctt aacgtgtcga ccctacgcta
    11281 aagtaatagc aacagctccg acaatgaagc aactttacga tgtactttgg gcagaggtag
    11341 ctaaatggtt aaatgatagc ttgatcaaaa acttactgaa gtggacaaag accaaaattt
    11401 atatggttgg tgattcagag cgttggtttg ctacggctag aacagcgact aaaccagaaa
    11461 atatgcaagg ttttcacgag gaccatatgt tgattgtggt agatgaagct tctggtgtgt
    11521 ctgatccaat tatggaagct attcttggta cgctatcagg ttttgataat aagctgttga
    11581 tgtgtggaaa ccccaataat attgaaggtg ttttttacga ttcccacaat tcagaccgtg
    11641 ataaatacag agttcataaa gtatcaagct atgatagtaa acgtacaaac aaagacaata
    11701 tagaaatgat tcttaaaaaa tatggaaaag aaagtgatgt tgctcgtgtc cgtatttttg
    11761 gagaatttcc caaaggtgcg ttggattcat ttatcagtct tgaaacggtt gaattggcta
    11821 cagaaaaaca aattagtgat tctttagtca ataaaacaac ggttgctcat attggtgttg
    11881 acgtagctcg atatggtgat gattctacga ttctctttcc tagaattgct accagggcat
    11941 tggagtatga gaagtattca aaacgtagca ccatggaaac aacaggatat gtcatcaaca
    12001 tggccaagaa tctaatgagt caatatccga gtattgataa agtgatgatt aaagtcgatg
    12061 acactggtgt cggaggtggt gtaaccgacc gcctagaaga acttatagaa gacaaacatt
    12121 atccttttga ggtgtttgga gtgaataacg gttcaacatc agaagacgat ttttacgata
    12181 atttaggtac tcaactatgg ggaaacatca aggaaatgtt agaagaaaat atgacagcaa
    12241 atcttaacgg agaacagcct gttattgaat tgccttctga tagttcgtta atcaaagaat
    12301 taagtactcg caaattcaaa atgacaagta gaagtcgtat acgtttagaa agtaaagatg
    12361 atatgaaaaa gcgaaatatt ggtagtcccg atattgctga cgcactggct ttagcgtttt
    12421 atgagccacc aagtcactat caatttattc aattttagga ggtgagcttt tgattagtac
    12481 agtattatct ttagagagat ataagaagct acgtgttaaa tatgcaacac aaattgaaga
    12541 tggaatgttt gatccgaatg gttttataga agatatgaaa ccattttttg ctgatcgcga
    12601 aagaaaatat ctagcttata ctagcgaaaa gaatgaaata gataatagac caaagcctaa
    12661 cactgatatt gtaaaagtta ataataaact tcatgctggt atgtattcaa ttgtcgtaga
    12721 ccaagcagtc aatcatttta ctggtatacc tatcaaatgg gattacgatg tatcggaaca
    12781 aaaaagaaca ctcattcaaa gattaaaaga taaattctta aaaaatgata tagaacttcc
    12841 gacagttcca gaagctttta acaaattaac aagcaatctt gattccatga gattcgcaat
    12901 gcttgattct gaaactgcaa cttttcaagg agcttgtgga gtagctttcc gattgttaga
    12961 gcctgtggaa gaagatgatg gttggaagtt aagagcaagt aatattgaac cttggagagc
    13021 agaaagatat ggaaatgccg gaatctatat caaagaaaag tatgactctt accagaaaaa
    13081 attttttcaa gaaatgaaag ttatgacaag aaataaaatt ctgacatatg cttgctatgg
    13141 tgatttgaat tttgttacaa gcggaacgtt caaaaaaatt gatgagaccg ataacccttt
    13201 ggggacgatt accttggcag aatttaaaaa taatacgaat cgttattgtg attttgaagt
    13261 agctgaggaa attggagatg caattgatcg tgctttgtct gatcaacaaa acgaaatcga
    13321 acaatttaag cttgcgtaca tgcttgttac tggcacaaca atgagcaaag gcactgctaa
    13381 agaaatgatg aatcagctag ggattatcaa cttaaaagac cctactgcaa aagctgaata
    13441 tgtcacaaaa aatttagcaa aagatttcaa tgaatatcat atggatctat tgaaaaaaca
    13501 gttttacacc atttgtaaag caatcgattt taacgatgag gtgtttaaat caaacagttc
    13561 tggagaagct cgcaagtggc aaatcattag cctagaagcg aaaacaaata ctaaagagca
    13621 atattttaga gaaggattga aagaatgcgc agaaacaatt gctgcttttc ttaagtttca
    13681 tgacaaagta gaaattgagc ctgaaaaaat tatttttact ttctctagat ctttgccaac
    13741 tgatttaagt tatttagctg aagctttacc taaacttgca ccatacgtat ccaaacgaac
    13801 tattcagagc caaattccat ttgtaacaga tgttgattat gaaaatgaaa tgatggaatt
    13861 ggaaagtgga agtgcttatc cagatagtga atacaatttt ggcggaggtg gcaatagtga
    13921 cgatagaaac gaagtattgg accaaacgtc gagaactgga ggatcaagca aggctcaaac
    13981 aagaaaatca gacacttaaa aaattaacta gtgtatttcc tgaagcactg aaagagatac
    14041 aagcaaaact attatcacaa gctgacttac acaatatcac ttatccagaa atgatggagt
    14101 tttatagtac aagtaatcag aaaaaatatc gtgaatatgt ggaaaaaaat tataagtcat
    14161 taaaaatgta tgatgcaaaa tacaaagagt ttatcgatga attttttcca ccatttgact
    14221 atgcaaaagt caatcgctta ttacaaatac gatcagatgt atttaaaatt cttgcagaat
    14281 atgcgatgga tgcagatgtg aatcaatatt tttctgatcg cttagaggaa attcttcaaa
    14341 gaacatattc ttctaatgct aatgtttttg ttcaactttt aaacgttgat ataccaaatt
    14401 atttaccaga aaatgaaatt aaacattatt tgaactatcc atggtcagga aagacatttt
    14461 caagaagact ttggggaaat atttcatcgc ttgaacagaa actctctaat gctattgtaa
    14521 aaagtgttgc tagtggagaa ggcgttatac acgcattaaa caccatgaga ttagactcag
    14581 aaatttgtga catgtttaag ttagaagaat caaagtataa caaagcgata gaaaatctcg
    14641 ttcgaacgga gtatgcaaaa tttgcacaag atggtattga aaaatcatat ttagaaacag
    14701 gtattgagga atacaacgta ttgactgcaa aagatgagag agtttgccgg atttgtggag
    14761 gaaaggcaag taagaatccc tataaactga aagatgctgt cataggtgaa aatcgagcac
    14821 ctttccatag tcgttgtaga tgtacggatg ttcctaattt accaaaatta ggaaaggata
    14881 ttgatgaaga atatgaccgt ttatttggcg atttattaga tgagtttgca catgattctt
    14941 ttggaattaa tttgaaacgg aggaagtaga atgaaagatt tttttgaagc agtactaaca
    15001 attaatgtaa atgctgatat tgcagaagcc tacaaaacag ctattgagtc tgagaaccat
    15061 cctaatggct tgagagacca ttggaatggc aattatgcct acgtggttat tggcgatcaa
    15121 actgttaatt atcaagataa tactccagtt gataagaata ccgttaattt aacgattcaa
    15181 ttattatctc attcattacc aaatttaaaa gaaacagttg attggtatga aaagatggga
    15241 tgtattgttg ttagaactga ctacaaagaa ggaaagtcta gtaattaggc ttttttattt
    15301 tgtccgaaat gacgttaaac tagcgcaata ctgggcttgg ttgaatggtg gggcgcaact
    15361 attaaaactc aaagcaatgc ggggcgtgaa aacgaatcgt ggggcgaaag gagaatgagc
    15421 atgaaacaca aatcattaat gccaatgaat ttgcaatact ttgccgaagg tgacgatcaa
    15481 aagttttctt ttgatgactt caaatctttt gtggaatcca atgaagaagc tcaaaaattt
    15541 attcagtcac aatctcaaag tgttgccgac aaacaattgg aagcttggaa acaaaataac
    15601 ttagataaaa ttaaacagga taccatcaag gaatatgagg aatctaagaa aaacaagtca
    15661 cctgagcaaa ttcaactgga aaaactacaa gctgaatttg aagcagaaaa agcgttgcgt
    15721 gtgacaagtg ataataaagc atttgttgca gaacaaattg ctggattaga actagatgga
    15781 gagttaaaag agtctatttc tcaatttatg ctaaataatc ttgttagttc ggatacagat
    15841 ttcactaaga atgctgttga aggttttaca agtgtcttga atgcaattaa tgagaaacat
    15901 gcagatgcat taaaagaact acaaatgaag tctgcatttg gtggaactca acaatcgaat
    15961 aaccaggttc agcagaacaa tgaaacatta acaaatccag aagaacaatt aggacaaatt
    16021 cttcaacaat ttaactagga gagtgaaaaa ttatgaaaaa aacatcttta aataatttag
    16081 agtatttgga tatttcacca gcgattaatg ctatgcaagt accaaataca ccttttttaa
    16141 gctatttatt tggtgctgga aaaacagagc cagcaaactc gacagaaatt aaatggcgtg
    16201 aatatgatat caacaacgat gattcttctg aaaaacttga gggcggagaa tatccagatg
    16261 ctgaatcagg tcgaacttgg tttaacaact atactgaaat ttttagaaaa tcaacctctg
    16321 tatctggtac attagatgct attaatgtga atggagtcgg aaatgaatta actaatcaag
    16381 tagcactacg tggtatggaa atgaaaattg atttgaaccg aaaattgatt actggtgtaa
    16441 aagctgatga aaatggttct aaaggtcgtc gaatgaatgg gattttgaac ttgatcaatt
    16501 cagcaaataa ggcagaaaca gccactgcgg gtgcagtaac aagaaaagat atcgatactt
    16561 tatttaaatt gatgtatgaa aaaggttata tgggagaaaa actatgcttg atttctccag
    16621 atatgcagga gttaatgact gatgagttag atggaaaatc aacaaaaatt gttcagttcg
    16681 gggaaagagt aacttttgga ttgcaaattg gaaatatcgt gtctaattac ggtacaggta
    16741 ttgctctact agaaccatca ttgccaaaag gaacaattgc cgcaatcgat actaattatg
    16801 tgaaactacg tccattacgt gaatggagag cagaagaact tgcaaaaaca acggattcta
    16861 gacgtatcgg tcttgtaggt gaatactctc ttgaatacaa tgcttcaaac tctggggcaa
    16921 ttttaaattt aaagtctgaa taaaagggag ttagtactcc cttttttgat aggaggaatt
    16981 acagtggtaa aaaaagatga aactaaaaaa gatgaagtcg tgaaatatag agtaggtaaa
    17041 actaaaaatt ttgttgggtt tgttcatcct aaaactcgta gatttatcac agcagattca
    17101 aataacgaat ttatcatttc tatagatgat aaagaagcaa ttgcaatttt ggaagatgca
    17161 attgatgtta atgaaattta ggaagtgatc tgatggatga atcgctaaaa acggaaatca
    17221 ttgagtctac aaaagaagat tttccagatt tgagtgaaga acgcataact aatttattag
    17281 aaataatttt gctagaaatt gaatcataca acacttgtaa aaatgatatt tcatgggaaa
    17341 agttaaaaag cgtgattagt gaagtgttgt ataaaataat aaaaaatgaa tcagaaaaaa
    17401 cagtatcttc agttagacgt ggcgatacaa cgattagtta tgcttcaaca acaaatgatg
    17461 ttagtgagtt acttctaggc tatggtgatt tgatacgaag agttattggt tgtggaggat
    17521 tggagttttt ttaatgaatg aagcagatat tttagaaact acttatgaag atagttgtat
    17581 tattgaaaga ctgacggaca ttgaagattc taatacaaat attactattc aagattacaa
    17641 aaaagtatat gataatccta tttcttgcgc tctttcacaa ggtcaaatcg atggactagc
    17701 agtcatagaa gatggagaga tggtaaatgt ttcaactgac acatataaat tatttgttca
    17761 tcctaagatt aaactaaaaa aaggagatcg aataacaata actcaaaagg ccagtggctt
    17821 aattttttct ctatttgcta ctaagccttt ttactatcct agtcattgtg aagtgaattt
    17881 gataggaagt gagaaaaatg ggtaatctca aatatgaatc aaatgcagaa aagatcattg
    17941 agaattttaa aaatatgact gtaattgctc aaaaagaagg aatatccttc gttaacgatt
    18001 caatgaataa agtcgttagt ttagctaaac cattaactcc tgtaaaatca ggtaatttaa
    18061 ggcgaggata tcgtgtagta aaagctagaa aactatcaag cggtcgaatt gttggagcag
    18121 ttataaacaa tgaacattat tttaaatatg tagaagaagg tcataggact aaaaatggtg
    18181 gctttgtcaa agggaaattc atgttaactc gtgcaacgaa tcttgcaaat atgtcttata
    18241 ttcctcgaag atttaaacaa atggcaataa aaatcgttaa gaaaggaaag taacatgaaa
    18301 gatgaaatca ttgctgcaat cagtagcaaa ttaaaagaaa tctatcctga tgggacaata
    18361 tatcttgatt cagttatgca gtcaactaaa gatttttatt tcgttttatc agtaatggaa
    18421 tctggaactg aaaatgtagg aattgatatt caaaatgttt ctttcttaat tgatattgca
    18481 ttgattgata ataaacctaa tagaaattta attaatgaat tagtctcacg ttgtgggact
    18541 ttttttaata cgattacaat cgatgagcaa atattatttc caaaagccta tttacctgat
    18601 gaagcagatg gtgttcaaca tattcgtttt acattagaat ttccacaata tattgaatgg
    18661 agtgaaagat aaatgggaga aaaaagaagt aaaaccggaa ttatttctgt cgaaaaacct
    18721 acttggtttc ctttggaaga tgagacaggg aattttccgg tatatggtac tgcgttgcca
    18781 atgggaacag cagtaagtat caaaccaaca gctaattatg aaacaacaca agattatggg
    18841 gattcagtgg ttcaagatca atttacagct tttggtggtg cagaagttga actggaagca
    18901 aatggatata ctcataaagt tttatctaca attactggag ggaaaattgt taaaggtggt
    18961 gccttgcgtt ctggagaaga tattgcgcaa gatggtgcgt ttgcatatag acgtaaaaaa
    19021 tcgaatggga aataccgtta tacggtcttt tataaaggac agtttgctct agattcagat
    19081 gaaacatcaa cgattgaagg aagtaaagta agttttactc atccaacttg gaagggttca
    19141 tttgtagatg taccaggact tggatatatg tattcagttg atgaagatga tgaaggcgtg
    19201 gataaagcaa tgatcggaaa ttggtttaca aaagtagcaa ttccaattga agaagcagaa
    19261 ctttcaggag gtacagaata atgtcaaaat atcaaacaac gattaaatta acgaaaaaag
    19321 atgaagaagg taaatatgag caagtacaat ttaaatctgc tgaattttta ccaggaacag
    19381 ttgtagaaga tgctgcagga gttatggaag aaatgcaaac agcgactgat aaacaatcag
    19441 ttaaaaaagc tttaagtcgt gcttattcat ttattgcaga tactttattt gagggacaat
    19501 ttactggtga agattattgc aaaggaattg atgctcgtga gattgcctca ttgacaggaa
    19561 aattattgaa gtctgttact gcaggttttg atgaaactta tacagagacg aaaaaaaagt
    19621 aagtgaggcg ctcaagtcac cttcatttaa gtattcgatt acttaccgag aattagatat
    19681 aaagacgcaa ttactcgaag caggttggac gttaccagaa attgaacgta ctgacttgga
    19741 tgagttaatg cgtctttttg cttttagaga tgcggtaaaa gagcatgaag atgttgagta
    19801 ctatgataat ttcactcaat tttaggaggt gatactttga ataacgatga cctaattctg
    19861 aagatgatac tagatgaatc tggttttact gccggtatga ataatgctgt caaaaagttg
    19921 aactcttttg atgaaacaat cgaaagaaaa agtagaaata gtggcagttc attaggtagt
    19981 atttggaaaa tatttgctgg aagttttcta gctagcggag taactagaat cgtaggagct
    20041 ggctttgatc taatcaaggg ttccataagt ggagcggttg atcgagtaga tacgatgaat
    20101 aacgctctac gaaatttcca aaatatggga tttagtaatt cagaaattat gaagaatatc
    20161 ggaaagaatg ggcttttatc tcaaggtatt caaggacttc ctactgcttt gaatgatgcg
    20221 ataagtcatg ttcaacttct tgcttcttct acaggtgatt taactcgttc aactcaaata
    20281 tttaaagctt taaatgatgg aattcttggt ttcggtggtt caactgatca agttaatgaa
    20341 gctgttattc aattatctca aagtttctca aacggaaaag tagacgcaca aacttggaac
    20401 tcaatgatca acgctcaact tggacctact ctttctgcta ttgctaaaaa gatgggaatt
    20461 acaatgggag atctgaaaga aggtttgtct caaggtaaga tttctgttga agaattccaa
    20521 aatcaattaa tagaaatgga taccaaaggt ggcggaggac ttaaatcatt aagtcaaatc
    20581 gctaaagatt ctactaaggg aattaaaacc tctatacaaa atgcaaaaac agctgttaca
    20641 cgtggtgttg gtgaggtaat agaaggatta aataaagcat tagtagattc agatttaggt
    20701 ggatttaagg gaattattga taaagtgggc agttcaatgg aatcgttctt aaaagtaatt
    20761 gctgcaaata ttcctacagc agtatcattt ttaagtaatt tatttaacga aattcaaaaa
    20821 tttggttctg ctttgaaatt catgatgccc tttcttgttc ctgcagctac cgcttttgga
    20881 gcgttcatgt ttcaacttaa aggtatacca gcaataataa aaagcttcaa taattttaag
    20941 aatgccataa ttggcgttgg aaattcatta aagattatgg gggcaatagc tgctgctaat
    21001 tcatttgttt taattattgg tgcagtcgtt ggtgcaatag ctgttttcgg ttactttatg
    21061 gcaaccaatg aagagtttag aaacaaggtt atatctattt ggaatgatgt gaaagattcc
    21121 gtaattggtg tattaaaaaa tataaaggac tggggaattg atacttggaa ttctgctaaa
    21181 gaaatggcat caaatgcagt tgagggtgtc aaagatgctt ggtcaggaac aaaggaatgg
    21241 ttttctaaca cctggaaagg tattaaggat ggggccacag gtttatttga taaaacagta
    21301 gagacttcaa gaaatgcagt cgatagtgta aaaaatgcat ggtctaatat gaagaaatgg
    21361 ttttctgata cttggcaaag tataaaagat tcagccagag aaaaatggaa tgaaattaaa
    21421 ggttctatta tgtcggttgc tggtccattg attacaggta taaagaatgc atttttacat
    21481 gttacttttt atctagagac cttatggaat aatttagttg agattggtaa aaacgtcttt
    21541 gagattttaa aaaatgttat acttgcgccg gttttgttta ttacctccat gatttctggt
    21601 ggatgggaag aaacaaaaaa taatatgatt gctgtttgga ataatattaa agaaagtgcc
    21661 ataaatattt gggaatctat aaagaatata tttgtaagtt attttacaaa tatttatttt
    21721 gctgcactca atatttggac agggttcaag cttacattga taaatatttg gaatgaagtg
    21781 gtaaatcaag ctaaatcgat ttggttaaac ataaagtatt
//
LOCUS       DAMYXM010000046        21291 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.047, whole genome shotgun sequence.
ACCESSION   DAMYXM010000046 DAMYXM010000000
VERSION     DAMYXM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.047"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..196)
                     /locus_tag="PNS21_001880"
     rRNA            complement(<1..196)
                     /locus_tag="PNS21_001880"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            937..1626
                     /locus_tag="PNS21_001881"
     CDS             937..1626
                     /locus_tag="PNS21_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI5159586.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1613..2821
                     /locus_tag="PNS21_001882"
     CDS             1613..2821
                     /locus_tag="PNS21_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDI5159587.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            3115..4386
                     /gene="serS"
                     /locus_tag="PNS21_001883"
     CDS             3115..4386
                     /gene="serS"
                     /locus_tag="PNS21_001883"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDI5159588.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4935..6419)
                     /gene="guaB"
                     /locus_tag="PNS21_001884"
     CDS             complement(4935..6419)
                     /gene="guaB"
                     /locus_tag="PNS21_001884"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDI5159589.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6924..7619)
                     /locus_tag="PNS21_001885"
     CDS             complement(6924..7619)
                     /locus_tag="PNS21_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HDI5159590.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7709..8809)
                     /gene="ychF"
                     /locus_tag="PNS21_001886"
     CDS             complement(7709..8809)
                     /gene="ychF"
                     /locus_tag="PNS21_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDI5159591.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8818..9009)
                     /locus_tag="PNS21_001887"
     CDS             complement(8818..9009)
                     /locus_tag="PNS21_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDI5159592.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(9030..9920)
                     /locus_tag="PNS21_001888"
     CDS             complement(9030..9920)
                     /locus_tag="PNS21_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDI5159593.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9907..10674)
                     /locus_tag="PNS21_001889"
     CDS             complement(9907..10674)
                     /locus_tag="PNS21_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDI5159594.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10686..11399)
                     /gene="rsmG"
                     /locus_tag="PNS21_001890"
     CDS             complement(10686..11399)
                     /gene="rsmG"
                     /locus_tag="PNS21_001890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDI5159595.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11631..13532)
                     /gene="mnmG"
                     /locus_tag="PNS21_001891"
     CDS             complement(11631..13532)
                     /gene="mnmG"
                     /locus_tag="PNS21_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDI5159596.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13549..14946)
                     /gene="mnmE"
                     /locus_tag="PNS21_001892"
     CDS             complement(13549..14946)
                     /gene="mnmE"
                     /locus_tag="PNS21_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDI5159597.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            15245..15628
                     /locus_tag="PNS21_001893"
     CDS             15245..15628
                     /locus_tag="PNS21_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159598.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15796..16554)
                     /locus_tag="PNS21_001894"
     CDS             complement(15796..16554)
                     /locus_tag="PNS21_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HDI5159599.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16572..17390)
                     /locus_tag="PNS21_001895"
     CDS             complement(16572..17390)
                     /locus_tag="PNS21_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDI5159600.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17387..17743)
                     /gene="rnpA"
                     /locus_tag="PNS21_001896"
     CDS             complement(17387..17743)
                     /gene="rnpA"
                     /locus_tag="PNS21_001896"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDI5159601.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17861..17995)
                     /gene="rpmH"
                     /locus_tag="PNS21_001897"
     CDS             complement(17861..17995)
                     /gene="rpmH"
                     /locus_tag="PNS21_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDI5159602.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18518..19852
                     /gene="dnaA"
                     /locus_tag="PNS21_001898"
     CDS             18518..19852
                     /gene="dnaA"
                     /locus_tag="PNS21_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDI5159603.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            20050..21180
                     /gene="dnaN"
                     /locus_tag="PNS21_001899"
     CDS             20050..21180
                     /gene="dnaN"
                     /locus_tag="PNS21_001899"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDI5159604.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         6067 a         4341 c         3909 g         6974 t
ORIGIN      
        1 gattgttata cggtattagc acctgtttcc aagtgttatc cccttctgat gggcaggtta
       61 cccacgtgtt actcacccgt tcgccactcc tctttttccg gtggagcaag ctccggtgga
      121 aaaagaagcg ttcgacttgc atgtattagg cacgccgcca gcgttcgtcc tgagccagga
      181 tcaaactctc ataaaaagtt cgaacaatct tgcgattgtt aagctcaatt tgtttgctag
      241 catattgctt gcttgttaaa aatgtttgtt gtcttgaatt gaatcaagac gccctacaca
      301 tttggtttgt cttactttgt tcagttttca aaggtctact tcatcaggag tgtttcccga
      361 ttcgtttttg gctgccgtag caacttttat atcatatcag agagacaagt aattgtcaac
      421 tcttttttaa aagttttttc gatttcgttt ttcgaaatct tccagcaact catttatcat
      481 aacttatcgt ttgttatttg tcaagaactt ttttgaagtt tttttcgata gaatcaagct
      541 tcgcaagtgg ttctttttta acgactcagt tatcttatca tgtcatttcc aacatgtcaa
      601 caacttattt tttattattt aataagttat tggtgttctt ttgaacaaat aataatatat
      661 caacatcaca tgtgaaagtc aataagaaaa tgcttattta tcaacatttc tagcttacat
      721 gatcattggt tagttctggt cctttttcgt tcgtctttat aactccgaga gacgtaaaat
      781 ccttttgttt tcagtttaat gttcatgttc ttgtcttatt cacaccaact agatgtctca
      841 ccttctaaaa ttttccttaa caaagtgctg cttttatcta tttctgcttt tttttgctag
      901 aataatagta acaatatgac tctggaggat ttatttatga aaattttgat tgctgacgat
      961 gataaagaaa tcgttgaatt attaagtatc tatgttcaca atgaggggta tgaagcagta
     1021 aaggcttatg atgggaaaga agcattatct aaaatcagaa caatccctga tattgaatta
     1081 cttattttag atatcatgat gcctgagatg gatgggatgc aagtagtgaa agagctacga
     1141 aaagaatccc aaataccaat cattatgctg acagcaaaaa ctactgatat ggataagatc
     1201 aaaggactgg tcgctggtgc agatgattat gtgaccaagc catttaatcc gttagaagta
     1261 atggctagag taaagtcttt attacggcgt acaaaaatgc agattgcccc agatcagcct
     1321 gatgtattag aagtaaatgc attaatcatc aataaagact ctcatgaagt taaaacaatc
     1381 gatgggaaag aaatccagtt aacagctttg gaattcggta tcctctactt gcttgctagt
     1441 catcctaatc gagtatttag cgcggatgaa atttttgagc gtgtttggaa acaagaaagt
     1501 attgtctctg ctaaaactgt catggttcac gttagtcatt taagagataa gattgaagaa
     1561 gcaactggtg gtgaaaaagt cattcaaacc gtttggggag tgggctataa aattgacgca
     1621 agatagtaaa cgagcaactg aaaaacgccg tcggatcaat ttgacttcaa aagaaatcag
     1681 cgaattgtta gcggaaggaa tcatcacgat tatccttttg cttctattaa atgtttctat
     1741 tctggttgtt atcagttcgg tgatcaacag ttcaccttct ttgacgaatg cgatttggga
     1801 ttcaaagaat atttttgcag aacgattgaa cactgatttg ttctggaacg gccggaattt
     1861 tattattccc tttttctttc tattagatat tggtgttttg tattggcgct tgattcgaag
     1921 atatcgccaa atgcaattgc ggcatatcat cagcgaactt cactacatcg caaacggaaa
     1981 ttatgatcat cgtatccctt ttgaactgag tggtgactta agccgagtgg taacaagtat
     2041 caatggattg gtggatagta ccgtagcagc gattgaagac gaacgtaaaa tcgaaaaatc
     2101 aaaagatgaa ttgatcacga atgtcagtca tgatatccgt accccactca cgtccatcat
     2161 tggataccta ggactgatag aagatggcca gtatcactca gaagaagatc ttttaaagta
     2221 tacgcatact gcttatatca aagcaaagca gatgaaatct ttagtggacg acttatttga
     2281 atacactaag gtacgccagc ctgctgtccc ggtgaatttt tctgcctttg acatgatcca
     2341 gttgatcgaa cagttagcag ctgattttga gttggaagct tctaaaaaaa atatccaaat
     2401 acttgttcaa tcaaaagtag actcccttat catggatggc gataccgaaa aacttgtacg
     2461 tgtcttcaat aatcttttga cgaatgcgtt gaaatacgga aaaggagcaa cgaagatcgt
     2521 aatcgaagta gaacgtatcg gttcagaagt tgttgcaaca gtcaaaaata acggagcaat
     2581 gatcccacaa caggcaatcg ataatctatt cgaccgtttt tatcgtgtag aagaatctcg
     2641 ttcacaagca actggaggaa ctggacttgg tttagcaatc gctcaaagca ttgtggcttt
     2701 gcacggcgga tacatttatg caaaatcaga taagcaatgg acttctttca tcatgcacct
     2761 tccaatcaag aaaaatgaaa aattacctat tgatacacag gaagttattg acgaaagctg
     2821 aaatattcgc tattcttatt acaattatta gaataaatga aaaacacaga acccagtaca
     2881 ctttattctt tttcagagag ctgatggcag gtgtaaatca gcaaaggata atttggaagt
     2941 tccagttcgt ttacgtgccc tttcgaaata agaaaggtcg gttcaacagc cgttatgtgt
     3001 tcaacaagtg cagctgacgc tgaacttggg tggtaccgcg aatttcagac tgattcgtcc
     3061 caagaataat gggatggatc agtctttttt tattttcaaa aaggaggaaa tcatatgtta
     3121 gatgtaaaaa tgatgcgaca aaatattgaa gaagtacaag aaaaattgat gactcggggc
     3181 gtaaaaaaag aaacattagc tgaattcatg gaactagatg aaaatcgtcg taagctacta
     3241 gtcaaaagtg aagaattaaa aaaatatcga aatgatgttt ctgctgagat cgcccaattg
     3301 aagcgtgcaa aagaagatgc ttctgctaaa atcgctgaaa tgaaagaagt cggtggcaac
     3361 atcaaagcat tggatactga aattgctgaa gtggatgaaa aattgaaaca aattgcaact
     3421 actttgccaa accttcccca tgattctgtg ccagttggaa cagatgaaga ggacaatgta
     3481 gaaatccgtc gttggagcga accacgtaca tttgcttttg aaccaaaacc acactgggaa
     3541 attgcagaaa atcttgatat tttagatttt gaacgtggcg caaaagtagc cggtagccgt
     3601 tttgtctatt acaaaggatt aggtgctcga ttagaacgtg ctgtctacaa cttcatgctt
     3661 gatcagcata tttacgaaca cggctatact gaaatgatca ctccttatat cgtcaacagt
     3721 aaagcgatgt ttggtacggg acaatttcca aaattcaaag aagatgtttt ccaattggag
     3781 aatactgatc taacattaat cccaacagct gaagtaccat taactaacta ttataatgat
     3841 gaaattttag atggtgcaga tttacctatt tatttcactg cactaagccc tgcttttcgt
     3901 tctgaagccg gaagtgctgg tcgggataca cgaggactga ttcgtcttca ccaattcaat
     3961 aaagtagaaa tggtcaaatt ttcggatgta gagcattctt acgaagaatt ggaaaaaatg
     4021 acagctgatg cagaagatat cttgaaaaaa ttgcatttgc cttatcgtgt gatggcctta
     4081 tcaactggag acatgggctt ttctgcagct aaaacttatg acttagaagt atggatacct
     4141 gcacaagata cttatcgtga aatcagttct tgttctaact gtgaagattt ccaagcacgc
     4201 cgcgcaatga tccgttatcg cgataatgat ggaaaaatcc actatactca tactctgaat
     4261 ggttcaggac ttgcagttgg ccgtacagta gctgccattt tggaaaacta ccaaaatgaa
     4321 gatggttctg tcacagttcc agaagcgctc gttccttata tggggaatct gaaagttatt
     4381 aaataaagag gctgtgaaaa agccgcttag tttcgagtat taagaaaaat tttgtgaaaa
     4441 tggctttcca catttttgca taaattggac ttaataccga gaaactggct tttgaacacc
     4501 atttatcgag aggttgtgaa atcagcgaat taacctgagt attagagaaa aaaatggaaa
     4561 aatagtccct catattttac cgtttgttga actaataccg agggtcagct gattgaacac
     4621 cgtttagtgg agaggtcgta agcctacccc ccagctccta gttacaagga caaattcaaa
     4681 aaacagctct tcatatttta tgaatgttgt acttgtgatt cggaggtggc aagggaacac
     4741 tgtttattca aaaagaggct gggacagaag cgtttaactc caagaaataa gaagaaattc
     4801 acgaaaattg cttttcaaat ttttgtgaat ttcttcttat ttccgaagga gttgcttcta
     4861 cttccgccgt ttatacgtgt ttagagcgtg agacaaaagt gttctttact tttgtcccac
     4921 gctctttaat gtttttaaga ttcaactgaa tagtttggtg cttcttttgt gatttgtaca
     4981 tcatgagggt gtgattcttt taatccactg ccgctcattt ggataaattg cgcttcatca
     5041 cgtaattgct gtagattggc tgccccaaca taacccattc ctgaacgcaa accgccgatc
     5101 atttggaaaa taatatctgc aacacttcct ttataagcaa cccgaccttc gattccttct
     5161 ggtactaatt tatttgcttc attgacacta ccttggaagt aacggtcttt tgaacctttt
     5221 tccatcgcgc ctaatgagcc catacctcgg tatgttttga atcgacgtcc ttggaagatt
     5281 tcgaattcac ctggtgattc atctgtccct gccaacatac ttcctagcat cactgcatgt
     5341 ccgccggcag ctaatgcttt gacgatgtca ccagaatatt tgatccctcc gtctgcaata
     5401 atcgctttgc catattgacg agcaactgat gctgcatcat aaatagctgt gagctgaggt
     5461 acacctacac cggctaccac acgtgtcgta caaatcgatc ctggtccgat accaactttt
     5521 acgacatcta cacctgcatc atataaagct tttgtcgctt cagccgtcgc aacgtttcct
     5581 gcaatcaatg ttgcatctgc aaaagttgaa cggatttctt gaatttttcg gatcacacct
     5641 gcactatgtc catgagctgt atcgatgatg attgcatctg cccctgcttc taataaagca
     5701 ttcgcacgct caaatgtatc gcttgttaca ccaacggctg ctgctacaag caatcgacca
     5761 tgttcatctt ttgctgcatt tggaaattcg atcactttct cgatgtcttt gatcgtgatc
     5821 aatccgctta aacgaccttc gttatctacg attggtaatt tttcgatttt atgtttttgc
     5881 aaaatctttt ctgcatcttt tagagaggta cctactgggg cagtcactaa gttgtctttt
     5941 gtcatcacat cagcaatcgg catgtgataa tctgttacaa aacgcatgtc gcggtttgta
     6001 attatcccta ccaattttct gttttcaagt gtttcaacga ttggcacacc actgatacga
     6061 tatttgctca tcaattcttc tgcgtctgct acaagatgct gtggcgttaa gaagaacgga
     6121 tcaatgatta cgccactttc agaacgtttg acttttctta cttcatccgc ttgctggctg
     6181 atcgtcatgt ttttatggat cacacctagt ccgccttgtc ttgccatagc aatggccatc
     6241 ttgctgtcgg taacagtatc catacttgca ctgatgattg gaatattcaa cttaatattt
     6301 ttagctaatt gtacgctcat atccacttcg tttggtaaca cgtgactttc tgctggtatc
     6361 aataagacat catcaaaagt aaatcctttt ttcgtgaact ttgtttccca gttggacatt
     6421 tttagtacca ctcctctaat ttagtttatc tattaaaata attgattttt tcatttaagt
     6481 caatgatcta cttggaatca agttagcttt ctcatagaat accaatataa agcgtttaca
     6541 tctgtgtgta ttaaaagaga ttgtaaaaaa gctgtcctgc atcaagtaat aagaacagcc
     6601 aatcaaaaac agcttctcat gtttttgaat tggttgggtt tattaccgca gatgcaaact
     6661 tttgaacacc gtttagtgga gaggctgtga aaaagctgct tagtttcgag tagtaagaaa
     6721 aattttgtga aaatggcttt ccacattttt gcataaattg gacttaatac cgagaaactg
     6781 gcttttgaac accgtttatc gtaaaaaggg actgtgacaa gacttttgtc acagtccctt
     6841 actattattt aactatggat aaacaaaatg aggttcttta ctacccctag atatctagca
     6901 aaatagctga tctttttgct agtttatctt cttgggacgg ataaacttcc aataatcccg
     6961 tattttttct tcaagtagta acggattgct aaagcagttg ccccgataat gataactact
     7021 actggatcta atactggatt gataactggt ggcaatagtg ttgtcgcaga aaacagtacg
     7081 acccaaagaa gtgtcgttcc acctaggata agcaatgtct tacccattcc tggtttcttg
     7141 cttcgatctg ctccgggata ttcatattga tagatgtatt tgtacatcat ataaaagacc
     7201 cagccaccga ttgcagaacc tagaacgagt gtagaaaagc cataagctgg cgttgttcca
     7261 cgtgaaaaca atccggtaat cgcaatcatg atacttaata gaccaagcaa cagcaacgta
     7321 ttatctaacc acatcaataa aggggtggat gaatttttta cttctgctgg cttattgatg
     7381 attgcttccg ttctttcaga gactgtccca aacaattgac gggctgttct tccgcctttt
     7441 tgttctgcaa ctaaagtagg cagaatgtca tgaagggctt ttactttttc ttcttcagac
     7501 aaattcgctg cttctaaaga ttttttcaaa tcaaaaatgt actgttgatt tcttttggtt
     7561 aattttgttt ctaaactatt gttttctttt acgattgctc gtaattgttc tggttccatt
     7621 atctaatagt cctccttagc cgttcctttt ctcaacggtc gtcacaaaag acattctacc
     7681 atattctaca gctagtttga tagccctttt acacattaaa gcggaataac attacgtcgc
     7741 cgtcttgtac gacataatct tttccttcta atcgaacacg tccagcttct tttgctgcat
     7801 gcatgtttcc gtaatgatcc aagtcttcaa aagagacagt ttctgcacgg ataaatccgc
     7861 gttcgaaatc tgaatggatg attcctgctg cttgtggtgc tttgattcct tttttgaatg
     7921 tccaagcacg tacttcttgt tccccagcag taaagtaagt agctaagcct aacaaatcat
     7981 aagcagcacg gatcaactga tccaaacctg attcttcgat tcctaaagct tctaaaaatt
     8041 ccgctttgtc ttcttcatct aattctgcga tttcttcttc tgcacgagca ctgaccacga
     8101 tcacttctgc attttcttct gcagcaaagt tgcgcacttc ttgtacataa tgattcgcgt
     8161 ctgcatctgc tacgtcatct tctgagacat tggctacata aagaattggt tttgtcgtca
     8221 ataaaaatag tgatttaacg attttttgtt cttcttcagt aaattcgatc gttcgggcag
     8281 attttccttc ttccagtaca ggtttgattt tatccagtac agctagttct gccactgcat
     8341 ctttatcttt ggttttagca acttttgcta cacgagtata gcgtttgttg atagattcta
     8401 agtctgctaa cactagttcc aagttgatcg tatcaatatc ttctagtgga tcaacacgtc
     8461 cttctacgtg agtaatatta tcatcatcaa aacagcgaac aacgtgacaa atagcgtcta
     8521 cttggcggat atggcttaag aattgatttc ccaaaccttc tcctttactt gcgcctttta
     8581 cgattccagc aatatctgta aattcaaaag tcgtcggaac agtttttttc ggatgtacaa
     8641 gttctgttaa gcgttgcaaa cgccagtcag gtacttctac catccctacg ttggggtcga
     8701 tcgtcgcaaa gggatagttt gctgcttctg ctcctgcttt tgtgatcgca ttaaacaaag
     8761 ttgattttcc tacgttgggc aacccaacaa ttccagcagt caatgccata aatttattca
     8821 ctctttctct gcattttttt caattacttt ttttaatttc ttttcaaaat ctcgacgggt
     8881 catcatgacg atatgtccgc aattcataca tctgattttg atatctgcgc ccattcgaat
     8941 gacttcccag cgattcgtct ggcaagcgtg aggttttttc atttcaacga tatcgtgcaa
     9001 gtcgtacata aaaatagcct cctttttgtt tattcttcat caaattgaat attcaagata
     9061 tcaagaattc gagtcaaatc tttttgagaa agatattcaa tctcgatctt acctttccca
     9121 tttttttctt ggatagctac acttgtacca aatttatcca tcaaacgatc ttcgctttct
     9181 cggatataat atggtttttc ctttgctagc cttgggattt ttttcttttc ttttccatca
     9241 ttttgattta actcagaaac aagctgttcc agttggcgga cagttaaatt ttcagttact
     9301 gcacgattgg ctaatttcaa gatcaatgtt ttatttttta aacctaacag cgtacgggct
     9361 tgtcccatag ataaacgctg atcttgtacc atttccttga caagatccgg taaagaaagc
     9421 aagcgcaaat aattggcgat ataaggtctg ctctttccta aacgactagc tacttctgct
     9481 tgtgttagtt tcaggttttt catcagcatc tcatatgctt ctgcttcttc taacggattc
     9541 agatcttctc gctgcaagtt ttccagcact gctacttgca tcatcgcttc ttcgtcaaag
     9601 tctctaacga tagcagggat cgtttctttt tcagctagtt tggacgctcg aaaacgtcgc
     9661 tcccctgcaa taatctcata tccttttact gcggaacggc gtacgatgat gggttgaaag
     9721 acacctgatt gttcaataga attagctagt tcctgcaaag atacttcttc aaatgtttta
     9781 cggggctgat acggatttgg tcgcagctca ttcaatggta gctggacaac ggtatcatct
     9841 tttacatcca ccgtctctaa gttttctaaa tcttgaaaca gtgcatcgat tcctcggcct
     9901 aatcctttac ttttcttcac gacttaccac ttcctttgcc aatgcttggt agacttcagc
     9961 tcctcgagaa cgtggatcgt agtcgatgat cggttttcca tgactaggtg cttctgataa
    10021 acgaatattt cttggaatga tcgtttcgta aactttttcg cggaagtatt tacgtacttc
    10081 ttctacgact tcatttccca gatttgttcg tgcatcatac attgtcagta agactccctc
    10141 gatctctaat tctggattaa agtgtttttg gactaaacga accgtattca gtaattggct
    10201 taatccttcc aatgcgtaat actcacactg aacaggtatc aaaatcgaat cacttgctgt
    10261 aaatgaattg attgtcagat ggccaagaga aggcgggcaa tcgatcaaaa tataatcata
    10321 ctgactgctt acttctgcta aagatccttt taatcttgat tctcttgcca tcattgacgt
    10381 caattcgatc tctgctcctg ctaattggag agtcgcaggc acaatactca aattttcgtg
    10441 ttcagtgatc aaagttgcct catcaattgg caattcattg actagaacat catagatgtc
    10501 tcttgtcaca tcaggttttc tgatcccgac accactggta gcatttcctt gtgcatccat
    10561 atccactagc aatacttttt tgccaaggct agcaagacaa gcacctagat tcaccgtcgt
    10621 cgttgttttg ccgacgccac ctttttggtt cgcaacagat attatttgtg ccatctcttc
    10681 tcctcctatt tgattggctg cttattaggc agtccgggct ttcttgggta tttctttggg
    10741 gttggtttct ttttttgaat aacgatgata tgccgttgat cagagacatt cggcaaggta
    10801 accgcctctt ctttgaggaa tttaccacct aataaagcaa tcgcttgttt tgcttctgtc
    10861 aattcctctt cactttttgc tgctttcaat gcataaaaat agccttcttg tttagccaag
    10921 ggcaaacaca attccgctaa cacattcaaa cgagcaaccg cacgagcagt gacaaggtca
    10981 aatgatgcac gaaagtgagg attttgccca aatgtttctg cccggtcatg atataaggaa
    11041 gcatcgattc ctaataatcg ggttaactca gataagaagg ttattcgttt attcagagaa
    11101 tcaacgatcg ttatctgcag ttctggaaag gcaattttta aaggtatgct aggaaaccct
    11161 gcccccgaac caacatcgca taaagataaa ggttgcgtaa agtctacttc aaatgctaat
    11221 gtaagtgaat cgtaaaaatg cttcagatat acgtcttttt catccgtaat agctgtaagg
    11281 ttcatctttt cgttccattc gactaatgtc cgataataaa tagcgaattg attcatttga
    11341 tgatcggtaa gctctatccc tttttctgcc aaggattggc gaaattcttc tggtatcata
    11401 aatattcctt tcattgtgaa acagcaattg tttcacagat tctctcccta ctttaacgca
    11461 aaatctctag acatgcaatc cttccatatg gattcttaaa aatttgaaaa gtaagaaaag
    11521 ccgatagact ttttctttct agtatgactc ataaaatata cacagaagag agaactagct
    11581 ttgagcaatt tctgcaaaaa ctagttctct cttctattta ttattacgta ttacgctggt
    11641 tcactgactt tcgcaatctt cccttgttcg atatagacca ttaggatact taggtctgca
    11701 gggtttacgc cactgattcg gctagcttgg gcaattgttt ctggttggat tttctgcaat
    11761 ttttgttttg cttctgttgc taatccgttg attgctgcgt agtcgatgtt ttctggaatc
    11821 tttttcgctt ccatgcgttt taatttttct actttttcca tggctttttt gatgtagcct
    11881 tcatatttga tttggatttc tacttgttcg atttcttttc gtgtcaattc ttcattttct
    11941 ggaataaacc gtagaagttc tgcataagta atctcaggac gtcttaaaaa gtcgcttgct
    12001 agtacaccat cttttaatgg tgccgcatta atcgttgcta aaaagtcttg gacttcttct
    12061 gttggtttga ttcgtacatt catcaaacgt ttgatttctt gttcaactgc ttcttttttc
    12121 ttcaaataat tcttgtattg atcttctttt actaagccta cacggtgacc catttctgtt
    12181 aatcgcaaat ctgcattatc atgacgtaaa agcagacggt attcagcacg tgaagtcagc
    12241 aaacgataag gttcgttcgt tccttttgtt accaaatcat cgatcatgac accgatgtaa
    12301 ccgtcacttc gtttgatcac taaaggttct tttccctgaa ctttcaaagc tgcattgata
    12361 ccggcaatca gcccttgtcc agccgcttct tcataaccgc ttgtaccatt tgtttgtcca
    12421 gctgtataaa gattttcaac gactttagtt tctaatgtcg gacggagctg gtgtggctct
    12481 actacgtcat attcgatagc atatcctgta cgcatcattt ctactttttc taatccagca
    12541 atagaatgaa gcatgttgac ttgaacatcc tctggtaaag aagtagataa accttgaaca
    12601 tagacttctt ccgtatttaa gccttctggt tccaaaaata attgatgacg tggtttgtct
    12661 gcaaaacgca cgatcttatc ttcgatggat ggacaatagc gagcgcctac cccttcaacg
    12721 atccctgtaa acattggggc acgatgtaag ttgtctcgaa taatttggtg agtcgtctca
    12781 cctgtatagg tcagccagca agattcctga tctagtttat aggcactatc tggtgtgctg
    12841 aagctaaaat gattcggctc tttgtcacca ggctgctctt ctgtaacaga atagtcgatc
    12901 gtacttgatt ttactcgagg cggtgttcct gttttgaacc gttcgatagc aaagccaagt
    12961 tcttttagat gattcgctag tccaatagaa ggttgagagt tattcggacc tgatgaatat
    13021 ttcaattcac cgataatgat ttctccgcga agtgctgttc cggctgtaat aacaactgct
    13081 ttggcccggt agctcgctcc tgtggacgtc actactccat gacaaacacc atcttcgacg
    13141 atcagttctt cgacgattcc ttgtcttagc gtcaaatttg gttctttttc gatcgtatgt
    13201 ttcatttctg ttgaataagc atgtttgtct gcttgtgctc gcaatgctcg gacagctggg
    13261 ccttttcctg tgttcaacat acgcatttgg atataggttt tatcgatatt cttacccatt
    13321 tctccgccta acgcatcgat ttctcgaacg acaacgcctt tagctggtcc gcctacagaa
    13381 ggattacatg gcatgaatgc taccatgtcc aaatttattg tcagtaataa tgtttttgat
    13441 cccatacgtg cagcagcaag tgcagcttct gaaccggcgt gtcctgcgcc aacaacgatc
    13501 acgtcatacg catctgcttc ataatgttcc aatcgtttgt tcctcctttt attttcctaa
    13561 acaaaattga ctgaataatt gagtgattag ctcatcttgt acactgtcgc cgacaacttc
    13621 tcctagatag tcccagcaac gcgtcatatc tatttgaacg agatccacag gcattcctga
    13681 atcaatacct gcaatgactt cttcaagtga gagggaagct ttttctaaca acgcgatatg
    13741 gcgggtatta gaaacataag tagcgtctct ttctcctgtt tctccgctga agaataaatc
    13801 agaaatagct gattccaaac gatctagtcc gtcgttttta gctacggaaa ttgaaaaaac
    13861 tggttcattt tcgatcaatt tcttcaattt ttcttgttcc agctgtgctg gcaaatctgt
    13921 tttatttaga agaatgatcc gtttcaatcc acttgtcgct tccaataatt gttcatcttc
    13981 tgctgtcagt ggttcgcttt ggtttagaac aagtaagatc aagtctgctt ctgctaatgc
    14041 ttttcggctt ctttcgaccc cgatccgttc caccacatct tctgtttccc gaattccagc
    14101 tgtatcgatt agcttgagcg gtaccccccg aacattgaca tactcttcga ttacatctcg
    14161 agtcgttcct gcaatatccg taacgattgc tttttcttct cttaacaaat ggtttaacaa
    14221 acttgatttg ccgacatttg gccggccgat gattgccgta cttaaccctt cccgcagaac
    14281 cttcccttgc ttggatgtag caagtagcgc ttggattctt tgttggatca tctgcgcttt
    14341 ttcaagcaat aacttcgtag tcaattcttc gacatcatca tattctggat aatcaatatt
    14401 tacttctact tgtgctaatg tctctaaaat ctcttgtctc aatgagcgga tcaaagcaga
    14461 cagattgccg tctaactgat taagagctag tcccattgct ttgtctgttt ttgcccgaat
    14521 caaatccatc acagcttctg cctgtgaaag gtcaacacga ccattcaaga atgctcgttt
    14581 tgtgaattcg cctggctcag ctaatcttgc accttctctt aacaatagct gcaaaatttg
    14641 attgactaca acgatcccac cgtgacaatt gatttctact acatcttccc gcgtaaatgt
    14701 ctttggtgct ctcatcacgg agaccatgac ttcatcgaca agctgattgc tcttgggatc
    14761 aacgatatgt ccataatgga tcgtatgact tggtacttct gataatcgtt tgtttccaga
    14821 ttgataaact ttatctgcta gttgcacagc ttgatcgcca cttaaacgaa cgatactgat
    14881 tgcaccttct ccaggtgggg tagaaattgc cgcaatcgta tcaaattcta aagtaatttc
    14941 agccattttt tcacttcctt tttcattttg cgcacaaaaa aagtgcccac tccacccttt
    15001 atgcacaata aacggtgaac ttcgcacttt gactgcttgt tcttgattaa attctggtca
    15061 tagattatca cacttgctat aaggaatcaa gtgtttaaaa caattagatg aagttgagcc
    15121 tgttctgctt ccattacctt atatttaatt gtccttggat gtctctttgc gttctttggt
    15181 ttattcgaag agaagatact gtataaacaa ggctttatct ttcttaaaaa aaacggtaca
    15241 ctaaatggta gcgctatcta aactatacac ttttgaaagg agaaaatatg tgagcccgtt
    15301 cagatctgct gcattggaag aagaaagtga ccctcggatc aacgatgtcg ttttatctac
    15361 agggaatatc aaccagaaat atatcgtccg cgatatcgtt tttgttgtgg aatgcttaga
    15421 agctgatttg tttgacccat cttttcccaa agatgacctt ttcttaacaa cgaagaggaa
    15481 attaaagacg aaggtacttg aatatggtgc gaatgcagta atcaactgtc attttgagta
    15541 tcagcaaaaa aaacgcgacg gaaagtattt tctggaactt tttgcttatg gaacagtgat
    15601 ccaatttatc caaacaacga ttggttaaat gaaacagaca aaaaaagcgg atcaaaactg
    15661 aagacagttt cgatccgctt ttttctatca aagttagtga ctacttcatc gtatactctg
    15721 ataagaatga tttctagtaa aatatacttg gtttgaaaaa ctttgtcaac gtgatttatt
    15781 caaaaagtaa tggaatcaat aatatttttt agcaggttct actactaaat aacgataagg
    15841 ttcctcacct tccgaatgtg tttttacatg ttcatctttg cttaatgcag catggatttg
    15901 tttacgttca aatgcaggca ttggttctaa gaaaacaggc tgccctgtcc gttttacttt
    15961 ttcagccgtg cgatcagcta aacgctgaat gatctcttgg cgcttttcac ggtaattgcc
    16021 tacattgact actacagaca gcttatttct tgctaaacga tggatgaaga cttgtgccag
    16081 atattgcaat gcattcagtg tttttccatg tttcccaatc agtatgcctt gcttttgtgt
    16141 ttctaaatgg aaaacaacca tattatttga ttctctttcc attttgacaa gagctggagc
    16201 atcaagctgt tttgaaattt cagtaagata aactgccaat tccgttaaag ctgcttcatc
    16261 acctagctca cttacttctt ctgttttttc ttcaagaatc gtttcgttga ctacctcgat
    16321 ctcagctgtt ggttcttctt ccaaaatttc ttctacagtc tgttctactg tttcagaaac
    16381 tttttcgctg atacttggct ggatcgaaag ccgtgcattc tttcgaccca ttcctaggaa
    16441 accttttttt ccttcttcca gtatttcgat ttcgacttgg tctttggcca aacccaaatc
    16501 agccagacct agttggacag cttcgtctat tgttgcagct tcataaatcg gcattagcat
    16561 tacctcccct attatttccg ttttttctta ggattcattg ctttcttaag agcacgttct
    16621 ctttcgcgga ttctccgtgc ttcttcttca cgctcttttc gaattttgaa agggttgttg
    16681 atcagcagcg tttgtacaac ttggaaggca ttggaaacta cccagtataa tgaaagaccg
    16741 ctggctaaat tgacacccat cactaaaatc attacaggca tcacgaaatt catgatcttc
    16801 attgatgcat tcgattcaat ttgactcatg ctagataaat acgtacttgc aaaagtaaag
    16861 attgctgcta aaatcggcaa aataaatgtt ggatccgcgt tccctaagtt caaccataag
    16921 aaatgccctt gtttcaactc tggtacacgt gaaatagatt gccataacgc catcaagatt
    16981 ggcatttgta ctaataaagg aagacaacca gcatatggat tgacgccatt ttctgcatat
    17041 aaacgctgct gctcttctct taacttactt tgtgtttccg gatctcttga actatattgt
    17101 tgctgcagtg cttttaattt cggttgtaat tcttgcgttt tacgcatgct ctttgtttgg
    17161 aaatgcatca acggcaatag gatcacgcgg atgatcagtg taaataaaat gatccctatc
    17221 cctgcattac caaatgacaa cgccttgatc gcctcagcaa agtaataaac aatataacga
    17281 tcccaaattc ctgtactctg tgcgctgact tccccagttc cgcaaccgga taagacgatg
    17341 accaaactaa cgataccggc catcaaaagt aaacgtttgt atttcttcac ttactctttt
    17401 cctctctgtc gattattttt gcaagtttta agacgtggac taaattggag cgaacctcat
    17461 cataagtcaa ccccttcaca cttggtctag caatcacaat aaaatccaac tccggatcaa
    17521 tttcttcttt tatagaataa actgctgctc ggatttttcg tttgacttca ttgcggttta
    17581 ccgcgttgcc gacctttttt cctacagata aaccaactcg aaaatgaggc tgtcccggtt
    17641 tttccaaacg gtaaacgacg aatttgcggt tagcaaaaga atctcccgta tggaacacgg
    17701 cttgaaactc tcgttctttt tttactcgat acgcttttct cattagtttt ctttcctttt
    17761 ctatctctca cttacgctca taatcatacc ataattacga ttgtcctttc atcatttccc
    17821 taaaaaagag tataaaaaaa accactgaaa ccccagtggc ctaagctgaa agaacttttc
    17881 ttcctttgcg acgacggcta gctaacacac gacgtccatt tttagtactc atacgtttac
    17941 ggaatccgtg aactttttga cgtttacgtt tatttggttg gtatgttctt ttcatatatt
    18001 ccacctccaa aatgttctta tctattttat aaacacagac atactttgat agtataacga
    18061 caaagtagtg attttgtcaa tataaaatag aagatcgatt gttaaaactt atccacaaga
    18121 ttattttttt ctatgggaat cctatgtgga aaaagttatg cacagggatt atccacagat
    18181 aataaaaggc tggttttaca cagaaaacac acgtgtaaaa aaagtaatcc acaatcaggc
    18241 gatttcctgt gtattccgtc tcctaactat tggtatatct gtatttttta tccacagacg
    18301 caagaggaaa tctattggtt tttacgagtt ttccacctgt gcattagtgg tgtggaaaac
    18361 tttttttgac aactgtgttt ttctcttttt tctttttgtc gattgtgtgg ataattgtaa
    18421 ttaaaaaatt tttttcttcg gtttctgtgg aaaactattg aaaaaaatgc taaattagta
    18481 ctagttattc actttttaat gaaggaggag gccttatatg gtatccctcg atgctttatg
    18541 gaatgaatta aaagcaacat accaaaagga tttatcgcct gctagctata atacatggat
    18601 cgaaacagcg aaccctcgaa cccttgatca aaaccagctc gttgttgagg ttcctagcaa
    18661 gattcataaa gaatattggg aaaaaaacct ggcgacaaaa atcgttgaag tcggttatat
    18721 gttatcagga aatgagatta tccctcgctt tattactggc gaagaagcag agcaagaaga
    18781 agttatagaa gaaaaaaatc caaaagttgt ggcacctagt ccgctaaaaa aagccatgct
    18841 gaaccctaaa tatacctttg atacgtttgt cattggtaaa ggaaaccaga tggctcatgc
    18901 agcagcgctc gttgtagcag aagatccagg ttctatttat aacccgctct tcttttatgg
    18961 aggcgtggga cttggtaaga ctcacttgat gcatgcaatt ggtcatcaaa tgctgcaaag
    19021 tcagcctaat gccaaagtaa aatatgtaag cagtgaaact tttgctaatg atttcatcaa
    19081 ttcgatccaa aacaaaacag cagaagagtt ccgtcaagaa tatcgaaatg ttgatttact
    19141 gctagtggat gacattcaat tttttgcaga aaaagaagct acgcaagaag agtttttcca
    19201 tactttcaat gccttgtaca atgaagggaa acaaatcgta ctaaccagtg atcggttgcc
    19261 aaacgagatt ccgaaactgc aagaacgcct tgtttctcgc tttgcatggg ggctgtctgt
    19321 tgatattaca ccgccggatc ttgaaacaag aacagcgatt ttacgtaaaa aagcggcggc
    19381 tgaacgtcta gagatcccag atgacacact aagctacatc gcaggacaaa ttgattctaa
    19441 cattcgtgaa ttagaaggtg cgcttgttcg agtgcaagct tttgctgcga tgaatagtga
    19501 agatatttcg actagtctag ctgcagacgc actgaaaacg ttaaaatcag gaaaaggaca
    19561 tcctcaattg tcaatcctgc agatccaaga agaagtggca aagtattatc acatccaatt
    19621 gaaagatctc aaaggaaaga aacgcgtcaa atctattgtt gttcctagac aaatcgctat
    19681 gtatttggca cgtgagctca cagataattc tttaccgaaa atcggagctg aattcggtgg
    19741 caaggatcat acaacggtta tccatgctca tgaaaaaatc cagcagctga tggattctag
    19801 tgtcagtatg caaaatgaag tatcagaaat caaaaacttt ttactaaatt aatttgtcga
    19861 ttctatcaac tgaaaatagc atgtggataa aagaaaaaaa cgttcaaaag ttatacacac
    19921 tttttccaca gctgtttttt cgtttctctc ttacgttttt tgagttttcc acagaatcaa
    19981 catgccctat tacttttatt attcttttta actaataata aataaacaaa cattcaaagg
    20041 agatacatca tgaaagttac tttaaaccga gctagcttta tgcaggaatt gcaaactgtt
    20101 caacgagcta tttcaagcaa aaccacgatc cctattttga caggtgtaaa aatcacactg
    20161 acacaagaag gtttgacttt gacggggagc aacgctgata tatcaattga aacttttttg
    20221 tctgttgaaa acgaaaaagc aaatatgcaa atcgaatcta ctggttccat tgttttacaa
    20281 gcacgtttct ttagcgaaat cattcggaga cttcctgaag aaacatttac tttagaagtt
    20341 ttagaaaata aacaagtagc gatcacttct ggaaaagcga attttatcgt aaatggatta
    20401 gatgcagata actatcctca tcttcctgtt gtcgaaagcc ataaccagat gaaattacct
    20461 gtacacgtat tgactaaact aatcaacgaa acagtttttg ctgtctcaca acatgagagt
    20521 cgtccaatct tgacaggtgt gcatttcatt ttatctgata attctttatt agctgtagct
    20581 actgattctc accgtctaag tcaacgcgtg attccagtag aacaagcggc tgatcatttt
    20641 gatattgtta ttcctggaaa aagtttgatc gaattatctc gctcattaac aaatgaagaa
    20701 gaaatcgtcg aaatcagcat tatggaaaac caagtgttgt tcaaaacaga aacgatgtat
    20761 ttctattccc gtttgctaga aggaaactat ccagatacca atcgtttgat tccttcaagt
    20821 tttaatacag aggttgaatt ttctgttcct agctttttag cggcgatcga acgggcttct
    20881 ttactttctc atgaaggccg taacaacatc gttcgtttat ctattcgtcc agatgcggtt
    20941 gttttatacg gaaattcgcc agaaatcggg aaagtagaag aaagcttgag ttatacagca
    21001 agcagtggcg atccattaga tatttctttc aatccggatt atatgaaagc agcgttgcgt
    21061 gcatttggag atatgagcat caaagtgaaa tttatctctg ctattcgtcc atttacattg
    21121 gaaccaacgg aagatggcgt tcaatttatt cagctgatta ctcctgtacg tacaaattaa
    21181 caatgatttt caatgttctt gaaaatttaa ttgaaaagaa atagaaaaaa taaaatttta
    21241 aacagaaggc cctaaaatct cttttaaggc tttctgttta tttttgttta a
//
LOCUS       DAMYXM010000047        19817 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.048, whole genome shotgun sequence.
ACCESSION   DAMYXM010000047 DAMYXM010000000
VERSION     DAMYXM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.048"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            257..532
                     /locus_tag="PNS21_001900"
     CDS             257..532
                     /locus_tag="PNS21_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159605.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2617
                     /locus_tag="PNS21_001901"
     CDS             545..2617
                     /locus_tag="PNS21_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDI5159606.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2782..4668
                     /locus_tag="PNS21_001902"
     CDS             2782..4668
                     /locus_tag="PNS21_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDI5159607.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4680..5627
                     /locus_tag="PNS21_001903"
     CDS             4680..5627
                     /locus_tag="PNS21_001903"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDI5159608.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5647..6174
                     /locus_tag="PNS21_001904"
     CDS             5647..6174
                     /locus_tag="PNS21_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDI5159609.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6237..6890)
                     /locus_tag="PNS21_001905"
     CDS             complement(6237..6890)
                     /locus_tag="PNS21_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDI5159610.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7022..7864
                     /locus_tag="PNS21_001906"
     CDS             7022..7864
                     /locus_tag="PNS21_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDI5159611.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8022..8918
                     /locus_tag="PNS21_001907"
     CDS             8022..8918
                     /locus_tag="PNS21_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDI5159612.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8921..9634
                     /locus_tag="PNS21_001908"
     CDS             8921..9634
                     /locus_tag="PNS21_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HDI5159613.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9654..10172
                     /gene="msrA"
                     /locus_tag="PNS21_001909"
     CDS             9654..10172
                     /gene="msrA"
                     /locus_tag="PNS21_001909"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDI5159614.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10169..10396
                     /locus_tag="PNS21_001910"
     CDS             10169..10396
                     /locus_tag="PNS21_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDI5159615.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10548..11099
                     /gene="lepB"
                     /locus_tag="PNS21_001911"
     CDS             10548..11099
                     /gene="lepB"
                     /locus_tag="PNS21_001911"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDI5159616.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11182..11682)
                     /locus_tag="PNS21_001912"
     CDS             complement(11182..11682)
                     /locus_tag="PNS21_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDI5159617.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11961..12359)
                     /locus_tag="PNS21_001913"
     CDS             complement(11961..12359)
                     /locus_tag="PNS21_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI5159618.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12619..13479
                     /gene="ylqF"
                     /locus_tag="PNS21_001914"
     CDS             12619..13479
                     /gene="ylqF"
                     /locus_tag="PNS21_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDI5159619.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13472..14239
                     /locus_tag="PNS21_001915"
     CDS             13472..14239
                     /locus_tag="PNS21_001915"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDI5159620.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14295..15152
                     /gene="dprA"
                     /locus_tag="PNS21_001916"
     CDS             14295..15152
                     /gene="dprA"
                     /locus_tag="PNS21_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDI5159621.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15274..17352
                     /gene="topA"
                     /locus_tag="PNS21_001917"
     CDS             15274..17352
                     /gene="topA"
                     /locus_tag="PNS21_001917"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDI5159622.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17321..18697
                     /gene="trmFO"
                     /locus_tag="PNS21_001918"
     CDS             17321..18697
                     /gene="trmFO"
                     /locus_tag="PNS21_001918"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HDI5159623.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18895..19800
                     /gene="xerC"
                     /locus_tag="PNS21_001919"
     CDS             18895..19800
                     /gene="xerC"
                     /locus_tag="PNS21_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDI5159624.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT         6873 a         3414 c         4121 g         5409 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa
     2641 gttttataaa aaacaaaaca tatcagaaca ggatcgaatc ggtcctgttc tttttttgag
     2701 aacacaaaaa gaacaaatac caaaaaaatt ttcctgaagt tcactatttt tatgatacaa
     2761 tatccaagat aaggagggaa catgttgaaa gaattaaaag taactgacct caaaaaaact
     2821 tatggtgaaa aagatttgtt tgaccagatt tcttttttgg tccacgaaaa agaccgtatc
     2881 gggttgattg ggacgaacgg gacgggtaaa acaagtctct taaatattat tgccggtatc
     2941 gatagcagcg acggtgatcg gcaaacagtg ttttatccaa ctgattaccg tattggttat
     3001 ctttctcaag cagctgaatt ttccgatgaa ttaactgttc tccaagcagt ctttcaagga
     3061 aacagcccct tgatccaaac tgttcgagca tatgaggaag cattgatcga actaggggaa
     3121 aacggagaag accctgatgt gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa
     3181 gaagatgctt ggactacaga taccaacgca aagattattt tgcaaaaatt aggaatcagt
     3241 gaattagata aaaaaatcag tactttatca ggtggtcaga aaaaacgagt tagtctggcg
     3301 caagtgttga ttgatgaacc cgatttacta ttactagacg aaccaacaaa ccatttagat
     3361 tatgaagcga tcgagtggtt ggaaaactat ttgaaacaat accgtggcgc actattgatg
     3421 gttacccacg atcgttattt tctagataga gtagcaaacc gaatcttcga actttcattt
     3481 ggaaaattat acgagtataa aggaaattat gaagcttacg tcttagaaaa agcagaacgt
     3541 gatcgtgtag aagtagaaca agaagaaaag cgcaagcggc tttataaaca ggaattagct
     3601 tggatgaggg ctggcgtaca ggcacgtggc accaagcagc aagcacggat caaccgtttc
     3661 gaagatctaa aggagaactt gtaccaagta aaccaagaag atgatttaga gctgaaccta
     3721 gcgacacaac gtttagggaa aaaagtactg gaaatcaaag atggcagtta ccgtataaat
     3781 gaccaaacac tgctagaaca tttagattta ttgattcaat ctcgggaaag actaggtatt
     3841 actggtaaaa atggtgcggg aaaatcgaca ttgcttaata ttctagctgg cagaattccc
     3901 ctggatagcg gtacaatgtc tatcggtgaa acggttcatc ttgcgtatta tacacaagaa
     3961 aatgaagaaa tggcaccaga taaacggatg attgcttact tacaagaagc tgctgaagaa
     4021 gcaaaaacag cagatggcag tcaaatcgga gtagcagaat tacttgaacg atttttattt
     4081 ccaagattta tgcatgggac actgataggc aaactttcag gcggagaaaa acgccggtta
     4141 tttttgttga aactgctgat tcagcaacct aatgttttat tattggatga accgaccaat
     4201 gatttggaca tagcaacatt gacgattttg gaagattatt ttagaagttt tccaggtgct
     4261 gtgatcactg tttcccatga ccgttatttc ttggacaagg tagcagataa gttattagta
     4321 ttccaaggaa acggaaaaca agaactttat tacggaaaca tgtccagtta tttactgaag
     4381 caaaaagaga cccagcagcc tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct
     4441 gctgaaaaga agaaattatc ttacatggaa caaaaagaat gggaaacgat tgaagatgag
     4501 attgcagaac ttgaagaaaa aatctcattg ctccaagaag agatgaatca tcaaggagat
     4561 aatttcactc gattacaaga attgcaaaac gacgtatctg aaacagaagc acaacttgaa
     4621 gaaaaaatgg cacggtggga atacttgagt gaatgggtag aagattagga ggaaaaagga
     4681 tggaacaagc gtatcttgac ttaggaagaa aattgttgga agaagggcat cgtaaagaag
     4741 acagaacagg cacaggcaca ttaagcttat tcggatacca aatgcggttt gatctgcaaa
     4801 aagggtttcc tttgttaaca acgaaacgag taccttttgg attgatcaaa agcgaattgc
     4861 tttggttttt aaaaggagat acgaatatcc gctatctatt acaaaataaa aaccatattt
     4921 gggatgaatg ggcatttgaa cgctacatca aaagtccaga ctataatgga ccagatatga
     4981 ctgattttgg ccatagatgt ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga
     5041 gcaagctttt ttgcgagaag atcctaaccg atgattcctt tgctgaaaca tacggcgatc
     5101 ttggacacat ctacggctat caatggcgtc actgggaaac aaaagacggt ggctttatcg
     5161 atcaaatcaa acaagtgatc gaagcaatca aaaagactcc tgattctaga agattgatcg
     5221 tttctgcatg gaatccggaa gatgttccta gcatggcttt gcctccttgt catacgatgt
     5281 ttcaatttta tgttcaagag ggacggctaa gctgccaatt gtatcaacgc agtggggatg
     5341 tgtttctagg cgttccgttc aacattgcaa gttatgcgtt gctgactcac ctaattgcac
     5401 atgaaacagg tttagcggtt ggcgaatttg ttcacacatt aggagatgct catctttata
     5461 tcaatcatat cgaacaaatg aaagaacaat tgagccgaga aatacgcgca tttccgactt
     5521 tagttttgaa tcaggaaaag cagtcagtat ttgattttga gatggaagat atccaattag
     5581 aaggatatga cccacatccg gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat
     5641 gagaaaatgt tagctgcaat ttgggcacag gatgaaaacg gattgattgg aaaagaagac
     5701 cgtcttcctt ggcgtttgcc aaatgatctg aaattcttca aacaaatgac agaagcaaat
     5761 acactagtga tgggacgaaa aacattcgaa gggatgggta gccgtccgtt gccgaatcgt
     5821 cagacgattg ttttgacgag agattcttcc tataaagcag aaggcgtgca tgtcatgcat
     5881 agtgtggaag acgtgcttgc tctggaaaaa gaaacagacg gtatcttttt catcgctgga
     5941 gggtcagctg tttatcaaga atttctgcca ttttgtacga tattataccg tacagtcatc
     6001 catcatgtat tcgatggaga tgcttatttt ccacctgttg actggtctga ttggtcactg
     6061 atcaatacta gtcaaggaga aatggatgaa aaaaatcgtt atccgcatca atttgaaacg
     6121 tatcagcgta atgagaatcc tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc
     6181 tatggataga ctagctgtta tttagagctg tcccatccat agctttattt tttttattaa
     6241 gtaaagaata aaacagagaa gtacataaaa cctgcaccta acataacaaa caaatgccaa
     6301 acaacatgca tatagcggat agaattcaag ctataaaaga atgcgcctat cgtgtaagaa
     6361 agcccgcctg ctaccatcaa aataacacca gtcattccaa gagagtgata aagaggaaca
     6421 gcagccacga cacagagcca ccccataaaa atatagatga tcgtagatac ttttgatact
     6481 ctctcccgtt tatgcaacgt caacgcttta tatataatgc caagaatcgc aaacaaccag
     6541 attaatacaa ataaagtcca acctagccat cctcggatac tcagcaaaca aaaaggagta
     6601 taacttcctg caatcaaaag aaaaatcgaa ctatgatcga atacttggaa cacctttttt
     6661 gcttttgtaa aaatcaatga atgaaacaaa gtagacgtta aaaataaaag aatcatcatc
     6721 gatccataga tagcataaga gacaatatgg agggaggaat gcaagtgggc accttttact
     6781 aaaagaacca ctagtcccgc gatactcaat ccagtcccaa ttccatgggt gactgcatta
     6841 aacacttcat ttaatatttc atacgttcga ttaaatttcg ttttttccat gaaaacggct
     6901 cctttactag gtgtaattaa tgaaaaatca ttatttgtct gttatactaa aaatagaaat
     6961 tgtcttacat gtagtttaac atgaataagg catcttgtta aagaaagaga gagagaaaat
     7021 aatgacaaat gtaaaaatcg taacagattc ttcatgtacg atggaaaaaa atgtacgcga
     7081 tgctttaggc attcgtgtca tgccgctatc catcatgatc gacggaacag tatatcccga
     7141 tgatgaccaa ctagacggcg aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc
     7201 caaaacaagc caaccaccaa ttggagaatt catctcgatg tatgatgagc taggagaaga
     7261 cggcagtgaa gtgatttcga tccatatgac aaaaggcttg agtggaacag tagaagcagc
     7321 aagacaagcc agtaatctaa caaaaacgaa agtaacggtt atcgacagtg ataccactga
     7381 ccaaggatta tcttttcagg taattcgagc agctgaatta gcaaaagaag gtaaaacagt
     7441 agagaaaatc cttgaagaag tcaaaaaagt acaagacaat acaaaattat acatcggaat
     7501 atccacacta gataacttgg taaaaggtgg aagaatcagc cgaacaactg gtttgctgtc
     7561 tagtgttttg aacatacgcg tggtgatgaa tttcgcccat tctgaattaa tccctgtaac
     7621 taaggggcga ggaaagaaaa cgttcgataa atggtttaat aatttgaaaa atgaactgaa
     7681 agaattgcca aatgtgcgac aaatcggtat ttcacatgcg gatgcaaaag aattggcgca
     7741 agaattcaaa gagggtttgc aagaattatt cccggacatg catatccctg tactgcatac
     7801 gaatccggtt atcgctacgc atactggtaa agacgcgttt gcaatcatgt attatgtaga
     7861 ttagtcgaaa agaggataag ccctcagcct gaaaggcttg cagcttattc tctttttctt
     7921 tataaaatta ggaaaataat tgttttcttt tttcataaag actacctttt tgataaaatt
     7981 tttgataaca ttagaaccaa tcgaatacaa gaggtgaaaa aatgaaatta acgaaacgac
     8041 atttgatcct cgttttgttc ccattattaa ttgcgatcat catctatgca ggtttagctg
     8101 cttctatacc gaaagctggc ccactaatga aaccagtaac tactgtgaga gaagatccaa
     8161 ataaaaaaga aaaacttcac tacatagcta tcggagattc gctgactgaa ggagtaggag
     8221 atcaaaccaa gcaaggagga ttcgttcccc ttgtcgcaaa tgatctaaaa gaccgttata
     8281 atttgaccgc ggttgaaaca gaaaactatg gtgtaaatgg tgaacgaagt gaccagatct
     8341 taaagcgagt aaaaacaaaa gaagaaattc gaaacaatat caaaacagct gattttatca
     8401 cactgactgt tggcggtaat gatttgatga aagttatcca aaataattta ttcgggttaa
     8461 cggtcaattc ctttaaaaag ccactgaaaa actatcagga aaatgtcaca gaactattgg
     8521 aagaatttcg atccctcaat aaaaccgcac ctatttacat tttaggcatc tacaatccat
     8581 tttatttgaa ctttcctgag atcactgaca tgcagacaat tgttaacaat tggaacaaag
     8641 gaacagaaac ggtgacaaaa aaaatgccga actgctactt tatcccaatc aatgatttgc
     8701 tttatcaagg attgaatcag gaagtaggtg tcacagaaga aggaacagat caaacaacta
     8761 gcacgacaga aaatctaaat ggcatcaaaa ataatgtgct ctatgaagaa gatcatttcc
     8821 atccaaataa tttaggttat caattgatgg caaatgcagt gagagatcag ctgatcgaga
     8881 cacaaaacca atggttagag aaggaggatg atcaataaac atgaaaacaa gatctgacgt
     8941 atcttcaaac aaaacaaaga aaacaaatta ctggaaattc gccttcctta tccttcttgg
     9001 ggtagtactt ggttcgaccg tatttttagg cagccgtatt tttgctaatc gggaaccaga
     9061 actcccagaa ataccagctc ttacggaaag acaaggagat cctgtcttaa cgatcaattc
     9121 aaataaagaa aaagtcaatc aaatcatcag ctttttcctt agtgagtatc aaaaagatag
     9181 tgatattgaa tataaatttt atttggaaaa tgaagcacta ctcaatggta ctttcgaagt
     9241 actaggtttt cctatcgatt tctatttata ttttgatccg tatgtgatgg aaaacggaaa
     9301 tgtccaacta aaagctagaa gtctttctat cggaacattg agtttgccaa tcaaagatgt
     9361 gatgaatatg atcaagagaa actataaatt accagattgg atcgaagtca atacagatga
     9421 tctgactatc atgcttaggt tggatcaatt ccgaatgcaa aacgggatgt atattaaagc
     9481 agataaaatc gacttagtaa atgatgacat tcggtttagt ctatatttgc caaaagatac
     9541 ctctgatgct tcaaacaact caaataccaa caaaactagc accagcagta ctgaaagcaa
     9601 gacaaaagaa aataaaaaat catcggatga gtagtattag agggggaatc ataatggaac
     9661 gagcaatatt tgcaggaggc tgcttctggt gtatgatcca gcctttcgac acgcttcctg
     9721 gtatccatac agtgatgtca ggatatactg gagggcatgt accagatccg acttatgaac
     9781 aagtaaaaag ccaaacgact ggacatacag aagcagtgga gattatctat gatccaacat
     9841 tgatttcata tgaacaatta gtagaacttt actggcagca aactgatcca actgacgctt
     9901 tcggacaatt cgaggatcgc ggtgacaatt atcgcccggt gatcttttat ttgacagaag
     9961 aacagcggat cattgctgaa aaaagtaaga aacagttagc tgaaagtgga cgtttcgaac
    10021 agccaatcgt tacaaaaatc gaaccagccc aaccatttta tctagcagaa gaagaccatc
    10081 aagagtatta taaaaagcat cctgaaaatt ttaaaagaaa ccatgcacgc cgggctgcct
    10141 ttatcgaaga aaattggagg aacgaagggt gaatcgaagc ttttttcatt atttaatgac
    10201 actgcgaggc ggtggcacac atgatgcttt atctgttttc gctacagaag ccggtaaaga
    10261 catccaattt cctaagcaca gctctagcta cgaagaaatt tctaattatc tggaactgaa
    10321 tgtcgactat ttgccgtcca tggatatctt tgacgaagca tgggaaaaat atttagaaaa
    10381 taatcatgac atataagaga tagttttgta ctttatgatc aaaaaagttt tttgtccaat
    10441 agcctaattg aaagaattat ttttatttca gctatctagt cgcatttttt tgatattttt
    10501 cttataataa agaaagaatg aaaaaaagag ggatggattg atagagattg acaaaaaaac
    10561 aacgctatat taatcgcttc tggcttgtat tcaaatactt acttgtttct gtattccttg
    10621 cgttcatgtt gcggggattt ttattcatcc cggtaccagt ggaaggcaat tcaatggaaa
    10681 acgtgttgaa gcaaggtgat atggttgtga tggaaaaatt ttcggagatc agacgatttg
    10741 atattgttgt ttttcaatta gcagatggca cgatttatat caaacgagta atcggtcttc
    10801 caggagaaaa tgtcagctac caaaatgatc aattgaagat caatgggaaa gtagttaagg
    10861 aaccttatct aactaaaaac ctgaaatcag atcatgcaaa tgcatcctat acgacggact
    10921 ttacactaca agagctaacg ggacaatcca agcttccgga agataattat tttgtacttg
    10981 gagataatcg aagagtttca aaagatagtc gttcgtttgg cacgatcaat aaaactgata
    11041 ttctaggtaa agcaagattt gtctattatc cacttgatga aataaaatgg atccagtgag
    11101 aaacaataaa gcgtgcagtc tgttgagact gcacgcttta ttgtttaatc caaaatcagt
    11161 ttactacctt agtacgtaaa atcaaacagc tcgtttaaac acttttctag cctctaaagt
    11221 tttgataaat gaataaacaa ccattatttg aatagggatt tggatcatat tcctgatgaa
    11281 tctacctaga aaaattactt tgaaatctgc accaaacaaa acagacatca gccaagaatt
    11341 gataaaaagg ctgactggga tcataatcaa taattgggca gcgatgacgg aaggcaggtt
    11401 tatcttttta tgatataaaa ataagccatt aataaatcca ccaaggaaag ccgtgataag
    11461 aaaagcagct tgaaacgttc ctgaaccaaa tatcgtgaaa tttaaagtgt tcgacaacat
    11521 ttcaagcaaa ccagcccaga tcggtccata tagataaccc ataagcgcca tacttaaaaa
    11581 tccgaatgtg atttgaaaat aagcaggacc aatcgctatc ttgtataaga tgacatcaag
    11641 ggctaataaa agagcagagg tcgacaacaa tcttgcattc atttcgggca atctaaaaac
    11701 gtcttttgca atcaaagtgt tcaaaatatt ctctcctttg tcagttaata ataaaaggat
    11761 accataaaat caccaatgtg tcatgacaca ttggtgattt acatacactg gcatctaaca
    11821 attaaaaaaa gggtgagaag actgctttta taaactgtaa aaaagagatt gtacgaataa
    11881 aaaaaataga ggcggagaat acttatcgta tcatccgcct ttgttttttt gttataaaaa
    11941 acgtcagatt agttatctgc ttacatcttc atccatacgg gttctaatgt atttccgtct
    12001 agatcttgga cttctaatga aaacatttct tcttcaggca ttcccatatc tacttcgtgg
    12061 aaagtaccac cattttcttt tgcaatttga gcaaattttt ttacttcatc cgcgttttca
    12121 acactaaacg aaatcaaagc accacttgtt tttttattat cagctatttc tttatctttt
    12181 ataaattttt tataaaaatc atgagagaga agcattatcc aaaaagtgtc atcccatacc
    12241 attgaaccag cttcttcagt agaaaattct tcattttttt taaaacccag cttttcataa
    12301 aaagcaacgg aacgattgac atcagcaaca ggaaaattaa caaaaaccat actattcatt
    12361 tgtttcgtct cctttctgtt ttgttcgtct atcatctctt atttaggaaa cgctgtcaaa
    12421 aatttagatt catatataga aaccaaaata aaaatttgca tcatcaaaaa agtgaagatg
    12481 tgatagaatg aggactggtt cactttcact taactaatag atggaatttc tttataataa
    12541 aaaagtgaat ttatattgaa aaagaaaaaa acaagtaaag ccggggaata ttccggtatg
    12601 aaaggaatgg agagaacaat gaccatccaa tggtttccgg gacatatggc aaaagcccga
    12661 cgagaagttt ctgaaaaaat gaaatatgtg gacatcgtat ttgaattggt agacgcacga
    12721 ttacctttat cttctcgtaa tccaatgcta gatcagatca ttcagcaaaa acctcgcctt
    12781 gttttattga ataaagcgga tctggcagat ccacaacaga caaagctgtg gcaagaatat
    12841 tttcgtaaac aaggccatct tgcattagct atcacggcac aagaaagtaa aggaattaaa
    12901 ggtctagtac ctgcggctaa agaagcattg aaagaaaaac gggcaagaga tgctgctcgg
    12961 gggatcaagc ctcgcgctat tcgggcaatg gtcttaggta ttccaaatgt tgggaaatct
    13021 acgttgatga atcgactagt aggcaagaaa attgcacaga caggaaataa gcctggcgta
    13081 acaaaaggtc agcagtggct tcgtttaggt aatgagttgg aattgttaga cacaccaggg
    13141 attttatggc ccaaatttga agatgaagaa atcggcaaaa aactagcgct gacaggcgcg
    13201 atcaaagacc agcttgtcca tttagatgat ttagcgattt acggattaga cttttttgcc
    13261 cgatattatt caggaagaat cagcgatcgt tatggtttat caatcgagca agaagaaaaa
    13321 ttggcaccgg aattactgat ggatattaca gcaaaacgtg ggtataaaga agactatgat
    13381 cgtggcagcg aattagtcgt gcatgatatt cgtcaaggaa aacttggacg atacacactg
    13441 gatcgttgcg aagaaatgga gcaaaaaagt gatgactgaa tccatccaaa gtatcaaaga
    13501 acgattgaaa agcatacaga gaatagatga tcctacacta gaatcattaa gaaatgatcc
    13561 acgcaaaggc gtgcagcaag cactggcttc ttttgaacga cgattgaaaa aggaagcatt
    13621 aattaaggaa aaatatgaag aaatgttttg ctttgaaaag caagggtacg cacaaggtca
    13681 ccaatggatt gccggcattg atgaagtagg aagaggacca ttagctggac ctgtcgtggc
    13741 agctgcagtt atcttgccga aaaatgagat catattaggg ctgaatgact ccaaacaatt
    13801 atcagagaaa aagcgagaac ttctttatca acaagttcaa gaaaaagccc tggcaatcgg
    13861 gatcgggatc gttgatcagc agacaatcga tgatgtgaat atttatgaag caagtaaatt
    13921 agcgatgatc caagcagtag aaaaattgtc cattgtacca agctatttgt tgatcgatgc
    13981 gatgaccttg gatttgccta ttcagcagga aaaaataatc aaaggggatg cacgatctgt
    14041 gtcgattgct gctgcaagta tcattgctaa agtatatcga gaccaattga tgaaagaata
    14101 tgacgtgctt tatcctggat atggtttctc gcataatgca ggttatggaa caaaggaaca
    14161 cttagaagga cttgaacgac aaggaattac gccaatccat cgtaaaagtt ttgcacctgt
    14221 aaaaaaatat ttacaataaa aaacagccgc ttttacgtat cttattagaa agagagacga
    14281 aaggcggttt tatgatgtat caaatagaag aaaatttatt gaaacttgct tatacaaaag
    14341 gaatcagtag tcaaggaaaa tgggcacttg ctaactggat gatgcagtat ccttataaag
    14401 aaatcggatt tgaagacatc attaagatag gtaaaataac caaacatcgg gagctgtttc
    14461 gcaaaagttg gcaggaaatt cacgctaact ggggcaaaat ccaatcgaaa cagtcgttta
    14521 tcacttgctt tgatcccaaa tatccccctc agctgcttca cttgacctat ccgccaattg
    14581 tactgtttta taatggtgat ctatctttac tttcttgtaa catgttaagc gttgttggtg
    14641 gaagacacac ttctgcttta gccaaaaaaa cagtttatta tttactaaaa ccagtgatag
    14701 aagctggata tgtgatcgtc agtggctgcg cgaaagggat agatacatac ggtcaccaag
    14761 cagcaatctc tcatggtgga cggacaatcg cagtcattgg aacagggata gagcaagtct
    14821 atcctgctga aaatcgtgac ttgcaagagg tgattgcaaa tgatcatctt cttttgagtg
    14881 aatatcctcc tcaatccggc ccgaaaaagc attgttttcc aatgagaaat cggattatcg
    14941 cagctttgtc taaaggaaca tgtgtctttg aagccaagaa aaaaagcggc tctctgataa
    15001 cagcacagca agcattggat cttggaagaa cagttttttc tgttccagga aatattttaa
    15061 cagaacattc taccggatgt catcatttga tccaagacgg tgcagtttgc acggtttcgg
    15121 gtgaagatat cttggcagaa tttaaagaat aacaccatac ttttcatttg cttttcttga
    15181 caaacgtttt ctgaatggat atgattagct acgatttgta tgaatcctct tttaatagta
    15241 ggaacacgac aatggaaagg agtccattca gtaatggcgt ataaatattt agtgattgta
    15301 gaatcacctg caaaagccaa gacaattgag aaatatcttg gacgaaacta taaagtaatg
    15361 gccagtgttg gccatataag agatttaccg aaaagtaaaa tgggtatcga ttttgaaaac
    15421 aattatgaac cccattatat ttctatacgc ggaaaaggcg atgtcatcaa aagcctgaaa
    15481 gccgcagcaa aaaaagctca aaaagtttac ttggcaagtg acccggatag agaaggagaa
    15541 gcgattgctt ggcatttagc gtacctactt gggttggatc tgaaagaaaa aaatcgggtg
    15601 gtcttcaatg aaatcacaaa agacgcagtc aaagcagctt ttaaggaacc aagaacgatc
    15661 gatgtagatt tagtagatgc acagcaagct cgtcgtacct tagacagaat cgttggttat
    15721 tcgatcagtc ctattctctg gcgtaaggtc aagaaagggt taagtgcagg acgtgtccaa
    15781 tctgtcgctt taaaaattat tattgaccgt gaaaaagaga tccgagaatt tgttccagaa
    15841 gaatattgga gcatcgacgg taattttaaa aaagctcgca agaaattcaa agcaaatttc
    15901 tggggaatcg acggtaagaa aaagaaatta ccagatgcac aaagtgtaaa agaagtcact
    15961 gctagaattg atggaacaga cttcgaagtt aaaaaagtag aaaagaaaga gcgcaaaaga
    16021 aaccctgcga atcctttcac tacaagtagc ctgcaacaag aagctgcccg taagttaaac
    16081 tttcgaacaa gaaaaaccat gatggtagca cagcaattat atgaaggaat cactttgggt
    16141 aaacaaggaa cagtcggctt gattacatac atgcgtactg attctacccg gattgccgat
    16201 acagcaaaag cagaggccgc agcatttatt gaagatacgt atggaaaaga attttctaga
    16261 catgatcacc gaaagatcaa aaatgctcaa ggcgcacaag atgcccatga agctgtgcgt
    16321 ccaacgagtg tgcttcgtac accagatgaa ctcaaacaat atttagataa agatcaattg
    16381 aaactttata cattgatctg gtcacgtttt gtggctagtc agatgacacc agctatttta
    16441 gatacaatga aagtgacatt agaacaaaat ggtgtcatct tcattgcgaa cggttcaaaa
    16501 atcaaattca aaggattcat gcaagtgtat gtggaaggta gagatgacgg caaagaagag
    16561 aaagaaaata ttcttccaga acttgaagaa ggcgatgtag tccaatctgt tgatattgaa
    16621 ccaaaacaac attttactca accaccagca cgttttagtg aggctacgtt gattcgtgca
    16681 ctagaagaaa acggggtagg gcgaccttcc acttatgcac caacacttga tacgatccaa
    16741 agacgctact atgtaaaatt gacacaaaaa agatttgagc cgactgaact aggagaaatt
    16801 gtcaattcat taatctgtga attcttccct caaatcgtcg atatccattt cacagcagaa
    16861 atggaaggag atttggataa aattgaagaa ggcacggaag cgtgggtcaa agtggttgat
    16921 cgtttctata aaccgtttga aaaagaactg acgaatgcgg aagaaaaaat cgaaaaaatt
    16981 caaatcaaag atgaaccagc aggattcgat tgtgatgtct gtggacatcc gatggtgata
    17041 aaattaggga aatatgggaa gttttatgct tgcagtaatt ttcctgattg tcggaatacg
    17101 aaaccaatcg taaaagaaat cggtgtgact tgcccagtat gtcatgaagg acaagtcatt
    17161 gaacgaaaat ctaagaaaaa cagactattc tatggttgtt cccgtttccc agaatgtgaa
    17221 ttcacttctt gggataaacc agtgggcaga aattgtccaa aatgtgacgg atatctggtc
    17281 gagaagaaag tcaaaggcgg taaacaagta gtctgtgtaa atggagatta cgaagaagac
    17341 atccaaaaat aatagaaaag aggttctttc attgaaaatg gtaaatgttg ttggagcagg
    17401 gcttgccgga agtgaagctg cttggcagat cgcacaagca ggagtccctg tccgtttata
    17461 tgaaatgcgt ccagtcaaat cgactgaggc gcatcacaca agtaattttg ctgaactagt
    17521 atgttccaat tcattaagag gaaacagttt ggctaatgca gtagggttat tgaaagaaga
    17581 aatgcgccga ttgaattcgg tagtaatcac ttcggcagat gaaacagcag tgccagctgg
    17641 tggtgcctta gctgttgacc gcgattcttt ttctgaaaca attacccgaa aagtcaaaga
    17701 gcatccatta gtaacagtga taaacgaaga aattactgaa ataccggaag gaatcaccgt
    17761 agtagcaaca ggacctttga cttctcatcc attagcagaa agcatcaaag cattcaatgg
    17821 ttcagatggt ttttatttct atgatgcagc tgcaccaatc gtagacaaag ctacgatcga
    17881 tatggataaa gtatatttga agtcccgcta tgacaaagga gaagcagctt acttgaactg
    17941 tccgatgaca gaagaggaat tcaaagcttt ccatgaggca ctaatcaatg ccgaagtggc
    18001 agaacttaaa tcttttgaga aagaaaaata ttttgaagga tgtatgccaa ttgaagtaat
    18061 ggcacagcga ggattcaaaa cgatgctttt cggacctatg aaaccagttg gattggaaga
    18121 tcctaaaact ggcaaacgtc cttatgctgt tatccagctg cgtcaagaca atgctgcagc
    18181 ttctttatac aacatcgtgg gctttcagac tcacttgaaa tggggcgagc aaaaacgtgt
    18241 tttccggatg attcctgggt tagaaaatgc tgaattcgtt cgctatggtg tgatgcaccg
    18301 aaacagcttt atgaattcgc cagaattgct gaaaccgact taccaatcaa cgaaacgcga
    18361 agatctgttc tttgcaggtc agatgactgg tgtagaaggc tatgttgaaa gtgcagcgag
    18421 cggactactt gctggaatca acgccgcacg cttagcaaaa gaaatggaac cagtcgagtt
    18481 tccacaagaa acagctattg gaagtatggc ttactacatt actcatgcgg aaggaaaaca
    18541 tttccaaccg atgaatgcca actatggtct gtttcctgaa ctgccagagc gcattcgaga
    18601 caaaaaagca agatatgaag caattgcaca acgagcttta aaagctaatg aggcaataaa
    18661 aacagaatta gaaaaaactg caatcccaaa tgagtaagga aactccatga agagggtgag
    18721 acggatagtc tcgctctttt tctggcgttt ttcttaagaa tttctcaaac aaaatgtgag
    18781 gaattgcgcc aattgtgaac aaacttagaa atattggtta aattctgaca atttatttgt
    18841 ctttttataa acgctgtgct aaattattct ctgggacaaa aggaaggtga gaaaatgaca
    18901 gaagccacgt gggaacaaaa atttttgcgt tatttgattg tcgaaagagg atattcagaa
    18961 aaaacgaaag aagcatatga agaagacatt ctccatttca aacatttttt ggaggaatct
    19021 ggagacgctg atctgctgaa ggcggaatat tttacggttc gtgtctattt gagtgcatta
    19081 gctgatcatc gtcctagcta tagcagaaat tctatcagtc gcaagatagc cagcctacgt
    19141 tcgttttatc agtatcttct caaagaagaa gtgataaaag aaaatccatt ttcttacgtt
    19201 catctcaaaa agaaaaattt gagactgcca cgtttttttt atgaaaatga aatgcaagca
    19261 ctttttgaca gcgtagcagg agatagtcct ttggatttga ggaaccgcgc tttactggaa
    19321 gtcctttacg ggtcaggcat ccgtttaagt gagtgtagca gtttgtcggt atcagacatt
    19381 gattttgatt cagaggtcat gttgatccat ggtaaaggga acaaagagag atatgctcct
    19441 ctaggttctt ttgctcaaga tgctctgcaa gaatatttca gggaaggccg ccaggtattg
    19501 atgacaaaat atcacgaaga gcatgatatc gtttttgtga atcatcatgg aaaaccaatc
    19561 acgcctactg gaattgaata tgtcttgaat caaatgatca aaaaaagcag tttggatagt
    19621 gatatccatc ctcacatgct gcgccataca tttgctactc atttattaaa taatggggca
    19681 gatttgcgga cagtccaaga attgctagga catgccaatt tatcgactac ccagatctat
    19741 gcccatgtaa caaaagaaag tttacaaaaa aactatcgtt ctttccatcc aagagcgtaa
    19801 aaataaggaa aagaaca
//
LOCUS       DAMYXM010000048        19739 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.049, whole genome shotgun sequence.
ACCESSION   DAMYXM010000048 DAMYXM010000000
VERSION     DAMYXM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.049"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            6..305
                     /locus_tag="PNS21_001920"
     CDS             6..305
                     /locus_tag="PNS21_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDI5159625.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="PNS21_001921"
     CDS             378..1739
                     /locus_tag="PNS21_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDI5159626.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="PNS21_001922"
     CDS             1761..2021
                     /locus_tag="PNS21_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159627.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="PNS21_001923"
     CDS             2035..2787
                     /locus_tag="PNS21_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDI5159628.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="PNS21_001924"
     CDS             complement(2881..3486)
                     /locus_tag="PNS21_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI5159629.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="PNS21_001925"
     CDS             complement(3539..4513)
                     /locus_tag="PNS21_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDI5159630.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="PNS21_001926"
     CDS             complement(4891..5610)
                     /locus_tag="PNS21_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI5159631.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="PNS21_001927"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="PNS21_001927"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDI5159632.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="PNS21_001928"
     CDS             7161..8558
                     /locus_tag="PNS21_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDI5159633.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="PNS21_001929"
     CDS             8639..9079
                     /locus_tag="PNS21_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDI5159634.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="PNS21_001930"
     CDS             9227..9553
                     /locus_tag="PNS21_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDI5159635.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="PNS21_001931"
     CDS             9554..10303
                     /locus_tag="PNS21_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159636.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="PNS21_001932"
     CDS             complement(10383..10838)
                     /locus_tag="PNS21_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDI5159637.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="PNS21_001933"
     CDS             complement(11265..11540)
                     /locus_tag="PNS21_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159638.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="PNS21_001934"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="PNS21_001934"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDI5159639.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="PNS21_001935"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="PNS21_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDI5159640.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="PNS21_001936"
     CDS             14770..17436
                     /locus_tag="PNS21_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDI5159641.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="PNS21_001937"
     CDS             17800..18552
                     /locus_tag="PNS21_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI5159642.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGGFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="PNS21_001938"
     CDS             complement(18574..19728)
                     /locus_tag="PNS21_001938"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDI5159643.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT         6287 a         3468 c         4052 g         5932 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcggatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctc
//
LOCUS       DAMYXM010000049        18293 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.050, whole genome shotgun sequence.
ACCESSION   DAMYXM010000049 DAMYXM010000000
VERSION     DAMYXM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.050"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            198..1679
                     /locus_tag="PNS21_001939"
     CDS             198..1679
                     /locus_tag="PNS21_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5159644.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(1782..2489)
                     /locus_tag="PNS21_001940"
     CDS             complement(1782..2489)
                     /locus_tag="PNS21_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDI5159645.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(2568..2906)
                     /locus_tag="PNS21_001941"
     CDS             complement(2568..2906)
                     /locus_tag="PNS21_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDI5159646.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(3017..3979)
                     /locus_tag="PNS21_001942"
     CDS             complement(3017..3979)
                     /locus_tag="PNS21_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDI5159647.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(3994..5325)
                     /locus_tag="PNS21_001943"
     CDS             complement(3994..5325)
                     /locus_tag="PNS21_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDI5159648.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(5333..6013)
                     /locus_tag="PNS21_001944"
     CDS             complement(5333..6013)
                     /locus_tag="PNS21_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDI5159649.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLIDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(6232..6711)
                     /locus_tag="PNS21_001945"
     CDS             complement(6232..6711)
                     /locus_tag="PNS21_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HDI5159650.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(6838..8517)
                     /locus_tag="PNS21_001946"
     CDS             complement(6838..8517)
                     /locus_tag="PNS21_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDI5159651.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            9065..10567
                     /locus_tag="PNS21_001947"
     CDS             9065..10567
                     /locus_tag="PNS21_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5159652.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            10580..11242
                     /locus_tag="PNS21_001948"
     CDS             10580..11242
                     /locus_tag="PNS21_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5159653.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(11343..11546)
                     /locus_tag="PNS21_001949"
     CDS             complement(11343..11546)
                     /locus_tag="PNS21_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDI5159654.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(11657..12841)
                     /gene="iadA"
                     /locus_tag="PNS21_001950"
     CDS             complement(11657..12841)
                     /gene="iadA"
                     /locus_tag="PNS21_001950"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDI5159655.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(12998..14737)
                     /gene="yfcC"
                     /locus_tag="PNS21_001951"
     CDS             complement(12998..14737)
                     /gene="yfcC"
                     /locus_tag="PNS21_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDI5159656.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(15510..16124)
                     /locus_tag="PNS21_001952"
     CDS             complement(15510..16124)
                     /locus_tag="PNS21_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDI5159657.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(16730..17107)
                     /locus_tag="PNS21_001953"
     CDS             complement(16730..17107)
                     /locus_tag="PNS21_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159658.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(17407..18288)
                     /gene="galU"
                     /locus_tag="PNS21_001954"
     CDS             complement(17407..18288)
                     /gene="galU"
                     /locus_tag="PNS21_001954"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDI5159659.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         5458 a         3871 c         3065 g         5899 t
ORIGIN      
        1 ttccgcctta aaaacaagaa tatcttgaca aaatacccta ggaaataaac aatagaataa
       61 acaaggtaac aaaaaaatta aataacgtta tataaaaaag aaggcgacac tctgtgagaa
      121 taacacagac tgaaatgact ttccactcac agtttttgtc taactatata caaaaacaga
      181 ataaaaagga ggtatttatg ctaattgaag caagaaaaaa agagttactt gttctacaaa
      241 tggtcattca aaaaaataat tgttctttac aagaattatc caatgactta catattccaa
      301 aacggacaat caaagaactg atccgaaaaa taaatgtatc actcaagcaa tttttattga
      361 ttgatgaatt tatttattct aatcataaag gagagatcca aaccaaagaa accgatcaaa
      421 ctaaaaagtt agctgttttt tctaagttaa aattatgcta cctaaaagaa tccaatcgtt
      481 ttaactattt gatgttacta atcaattatc cacaaactgc tgtaccaaaa aaatatttac
      541 ttgaacaact ttatatatcg ccctcctatc tcgaaaaact gacaaaacag ctcaatcatt
      601 ccttaaaaaa attccattta cggatcctat ctgtaaaaaa ttgctgtact cttgaaggaa
      661 atgagttatc tatccgtctc tatctttact tttttctttc agatacattt ggaggtaacg
      721 attggccatt ttcctcactt gaattaaatg cactaaaaat gaacagtaca gatgagcaaa
      781 tagaaaaaat ttcaaaacaa gaaaaaaata tctacttgct gttaacttta ttcatattac
      841 gaaacaaaca acaggcatat tttccaaaaa cttcttctga aatcactgaa ttgatggact
      901 tattgcagca tacacatgat tattcagaat actttacttc tttttcagtg agtcgccctt
      961 gtgaagagac agaaacagag agagcctact ttaattattt tttctattgt ttttttccaa
     1021 atcaaatctc ttttcgacaa aaacaggaga ttggtttttt attttccaca agcaaaaacc
     1081 ttatttgtcg acaagtcggt caattgatga cttacttaac tcaacagttt cctctcaacc
     1141 atttagaaaa taaaatacca atttattttt attttcttgt gttggcagtt gtttttttcg
     1201 aactgttggg tgaatccagc agtagttttt ctacactttg ctttccagaa gccacgtcca
     1261 atcattctag ttcttttata caggaaaaga aattaagaga aaaaatcgat acaatttttt
     1321 caaaaaatgg ggaaagaaac caaatcagtc aataccttag tcaattgatc caacacttac
     1381 tacaaacaga aacgaaagag cagttactca tccatttgca gataaccaaa cagtttacag
     1441 gaaattatca tcttcaaagc catttgcagc agttatttcg caaagatgct gttaaaatta
     1501 ccgcatattc agaacatgct gatttgatca ttacagatca gcatgagaaa atttcagatg
     1561 aaacggagac tttctatctg gatacaacgc acaatacagt gttatttggc aagttaataa
     1621 aagcggttca tgaactgtat atgaaaaaag gttatgagaa caaaacaaag aggatgtgac
     1681 aaaattcttg tcacatcctc ttattccaaa taaatggtat tcaatcagct aactctcggt
     1741 attagctcag atcaaagcgc tgatttcaca acttctttat tttaatctct tcggttgccg
     1801 ccgaacaata agatcaagcg caacagctgc aggaatgtcg aaagcgctgc tgcaacataa
     1861 gtcagtgctg ctgcaaataa tacgtgtctt gccatcggta cttcttcttc cgtcagtagt
     1921 ccgccttcac tcaaaatttg gattgctcgc cgagacgcgt taaattcgac tggtaatgtc
     1981 accaattgga aaagtaatgc taaagaaaaa gcgaaaatcc cgatattgat taacgtagag
     2041 ttccagctaa gcaatacacc taccataatc aaggggaacg aaatcatcga accaaagtta
     2101 gctgctggaa caagtgctgc acgaatcttc aatgggacat aacccgaagc atcttgtacc
     2161 gcatgtccgc attcatgagc tgctacacct atcgcagcaa ctgaagtaga ctgtgcagtt
     2221 gcttgagaca aactcaacat cttattacct gaattgtagt tatctgtgag atctccggca
     2281 atttgttgaa caccgacatt atttatctgt tgactttgta atatatattg tgctgcttgt
     2341 gtaccagtca cattgttttt gcttctcact tgatcgtatt ttcggaaagt actattgacg
     2401 tacgctgacg caattccgga aataactaat ccaagaatga ccaaaagaaa tgtcggatct
     2461 aaaaaaccat acattccata accatacatt ttcgtttcac tccttttcgt ttactttatt
     2521 ttatcattag tagtaggaaa agaatgtgaa aagaccttga atattcttta attctttttc
     2581 acaaattccg atttcagttt catcggtcca aatccttcta ccttgcaatc gatattatga
     2641 tcgccttcta ctaaacggat gttcttgact tttgttcctt gtttaatggc acctgacgca
     2701 ccttttactt tcaagtcttt aatcactgtc acgctatcgc cctctaacag acggtttcca
     2761 tttgcatcta ataccactaa tccgtcttct atgacagttt cttgttcttc tttgttccat
     2821 tcgttcccgc attctgggca aatcaacaag cctcgatctt catatgtgta ctctgagcca
     2881 cattctgggc aatttggtaa tgtcatcttc tttctccttt aaaatcttta gtgagcacta
     2941 ttgtaacgga aaaagaagat cttgaaaaga tacatttgtg atttttacta caaaaggcac
     3001 gttttcgaac gaagtatcat tctgtttttc tgctcttata cgattggcat atatcttgga
     3061 tctctttata gatttcctct acttctgcta aatcttttgc attcgcacca cttgctaatg
     3121 gatggcctcc tccatgatgg cgtttagcca attcattgat gatcggcccc ttagaacgta
     3181 aacgtacgcg gtaataccct gaaggctgct gaacaaaaat cccccaagcc agcacttcgt
     3241 caatcactcc aggaagtgaa acgatcgaag ctgtctctga atcatcgatt ccatatttat
     3301 caagaatttc ttgcggaagg attattcttc ctgcaccatt ttcatccacc tggatatttt
     3361 gataaagata accggaaagt ttagcgactt tcatcggcat ttgttcaatc tctctgttca
     3421 aagcacttgc atcaaatgga taatttctca atatcgaagc aatctctaat gtacgggacg
     3481 tcgtggaggg atacaaaaag cgccctgtat ctccgacgat tcctgcataa agtaaccgtg
     3541 cagccttaac gttcatcttt aaagattcag gaaacatcaa agcaaattct gcaatcatct
     3601 cactacagct acttgctttt gtatttaccc acacaagatc tccataaggg tcatcattgg
     3661 gatgatggtc gattttgatc agtttttctg ccaactgata tctttgatca ctgattctgg
     3721 gtgaattagc tgtatcagtc acgatgacta gcgctccttg gtagaagaca tcttcaactt
     3781 cttgcatttc tgctaaaaag cgaagccctt caatcgtttc acctacttga cgtatttctt
     3841 tttctgggaa actttcccgt aatagttcgg ctaatcctac ttgtgatcct aatgcatcag
     3901 gatcagggcg caaatgcctg tggataatga ttttatcgta tttttttatt gtatctacta
     3961 tttcttgctg tactgtcatt tcactcctgc tttctaagaa cgttccatta cttgacaaac
     4021 aacgattgct ttggaaacga tggtgttttc aataaacact tcgatatcca gtttagcgga
     4081 acgtctgccg atttccaata cacgtggacg aatgtctaat tcgctttcca attggatcag
     4141 ccgcaaataa tgtaggttca cttgttcaat caagatattt cgtttttggt tcatcaccat
     4201 tgttttttgt gcgacatcag aaatgatttc gctaagtacg ccaaaagaaa tggttccaac
     4261 gccattgacc atttgtggtg ctactacgaa tttgaactta gggttttcca aacgatttcc
     4321 ttcacggtca gtatcaatcg tatggatatt tccagatatc tgatccgaaa tcgtatctgc
     4381 aatctgcggt tgtcgctgga caagctgcat cgctttcatt acatcttgcc gggtaatcaa
     4441 gccttccaat gtcaagtcat cggaaacgac cggcatgact tctagcccat cccagatcat
     4501 ttgatggcta acagaggcaa tactcattgt tttctttaca ctgattggtt ctttcgtcat
     4561 cacccgatcg atgatctggt ttggcgattt ccctaaaaca tcttttgccg taacaatccc
     4621 catcaaacga tgatggcggt tgacgactgg aaaacgcgaa tgctgcgttt cttctgacaa
     4681 acgctgataa tcagaaatcg tatctgtctg ctgcaaataa tgagtctttt cagctggcat
     4741 atagatatca ctgacaagca tgatatcttt tttgatcaat tggtcgctta acgctcggtt
     4801 gatcatggtt gcgactgtaa atgtatcata agaggtgcgt aagatcggca tttcaagatc
     4861 atcagctagc tttgcaatct cagcagtcgt attgaatcct cctgtgatca atactgcggc
     4921 gccatcttcc aatgctaatt tttggacttc cgcccggttg ccgacgatca ttagtgatcc
     4981 tggcgtaata tagcgttcca tggcatgttt agtcatcgcg ccgatgacga atttattcaa
     5041 gaccttgtct aatcctgccg ccccgcctaa aacgtcccct tcaatgatcc tgacgacctc
     5101 gccaaatgtc aaacgttcaa tatgtttttt taatttgcgt tcgatacgga tggtccctac
     5161 tcgttgaatt gttgaaacta gtccgacatt ttctgcatct ttgattgcac ggtacgctgt
     5221 tccttcactg acattcaatt cttttgcgat gcttcttaca gaaattcgat tgccaacagg
     5281 taaactttca atatattcta aaatcaaatc atgttttgtc gccattttct gcctacactt
     5341 ccagcgtttc cgagacgctc attatttttc cttcactatc ttccagtaac tctacaaaag
     5401 attcgggatc ttgttcaatg acagggaaag tattgtaatg gattggaact actttcttgg
     5461 cacgaattgt ttgcgcagcc tttgctgcat cttctggccc catcgtatag ttatcgccaa
     5521 tcggcaagaa agcgacatca atatcgaatt cttctgcaag caatcctaga tcactgaaat
     5581 aagcagtatc ccctgcatga taaatcgttt ttccttctgc ttgaataata attccgctag
     5641 gctcgcccat atactgaacg ataccgtcta cttcatagct tgaactatgc tgtgcatgta
     5701 cgaattttac cacgccgaat ggaaacgcat gttttccacc gatattcata ccgaatgaat
     5761 ctactcctct gctgttcgca taatcagcaa tctcaacaat actgataatt gtggcttgat
     5821 tttcttttgc aataggaatc atatctccta tgtggtcact atgaccatga gtaatcagca
     5881 accaatctgt ctttacttct tctgcagtta agtctatcaa tggatttcct gtaataaacg
     5941 ggtcaatcat cacgcgttgg ccattttcca tttcaattgt taaacaggaa tgtccatgat
     6001 acgtaatttt catattttac acctcgcgtt ttctctgtat cttttttatt aaaaacgatt
     6061 tcatcacttg cacatcaatg aaaaaatata gctgctaaat atattttcat tggattgata
     6121 gtacagtatg tacaaagcaa ttgtaacaga taatacagga tgcaccaagt accaagcaga
     6181 tataatctca agtttgaggt ctctgctctg cttagcttct cttctcgtcc cttaactaat
     6241 taaatcagcg taaataggat ggacgatctt ttctaaatcg gctactttta tccgaaggct
     6301 tctcccgatt tcaccgcttg acacaattac ttcttctttt tcttttaaag aactgtctgc
     6361 taaaaaacaa taatcaggat gtgcgagttt tattccaata ggggtgtttg ctccgtgggg
     6421 atatcctgta tgcattaata caaaatccat tccaggaaaa ccaactttac gatctttcgt
     6481 cacttgtctc gtttttttgt aatctaagtg atcgttcaaa ggaatcaatg cgatgatcac
     6541 cccagttttg tttcctttca ctacgagagt tttacatatc aaagctggat caatccctct
     6601 tttttgtagt tcttctgtga aagattcttc taccacatga ctaaaatcaa gtggttgata
     6661 gctgatcttt tgttcttcta aataacgttc gacttttgtt tgactcatca ttgattcact
     6721 ccctttattc tatcgtttat tataaagcga gggatgctgt attcaaaaat tatttgtttt
     6781 tcgcaaaaaa agagcaagac gagcactcta tagagaggtt catcttactc tttttgttta
     6841 ttttttattt cgacgttgag tagcttccat aatgactggc aagatcactg ggcggcgttt
     6901 tgtttgttca aataagtaac ggcctaattg atctcggatg tcttgcttta atttgctcca
     6961 ctcaaaatca ttgctttcta aatgtttctc aacgattgtt tcaatcatat tgctgctttc
     7021 tttcatcaga tcacggctag cttttacgta aacgaatccc cgtgaagtaa tttgaggttt
     7081 tgaaatgatt ttcttttctc gccgactgat agtgacaacc gctacgaaaa tcccgtcttc
     7141 tgacaagatt tttcgatcac gtaaaacaat gtttccgata tcacctatgc ctaatccatc
     7201 aatcattaca ttttctgcac ttgtagttcc tgcaacactc atacgcttgt tcttatattc
     7261 cagcacgtct ccgcgtccag taatatagat atttttgtat ggcatcccta ctgcatgagc
     7321 caaatctgca tgagcagcta attcacgata ctctccttga actggcacaa aatatgttgg
     7381 tttcatcaaa ttgatcatta attgcaagtc attaggattg gcatggccag aaacgcgcat
     7441 attctcagag atcaacttca ctgttccacc agctcgataa atgatatctt ccgttttcgc
     7501 cacgatggtt tccatcgcga tgcttggcgt agtagtgata taaaccaaat ctccttcttc
     7561 aatcttgaca ttacggtgtg taccgtttgc cattttttgc agagacttga tgggttcacc
     7621 cattcggcca gtttctaaaa tgatcagttc ttctggatca tagtttttca tgtctttcaa
     7681 cgtaacaagc agatcttcac ttggtaattg aagtttttct aaacgcatcg ctgtgcgaat
     7741 aattcgttcg aaatcttgac cagtcaagac aacttttcgt tgtgcttttt ctgctgcatc
     7801 caatacttgt tgcactcgtt gcaaattact tgctacacaa gcgacgatga tacgcccttc
     7861 ccaatatttg atcgtatcaa atacttcatc tgctatttgg gcttcagaag ctacaggctg
     7921 aggattttct gcatttgccg attcacttag taaggctaag actccttctt tgccgatttc
     7981 cgccaatcgt gcataatctg tttgatacat tgggatagcc gtttggtcaa atttaaaatc
     8041 tcccgtataa acgatactac cttcttttgt tttcagattg atccccactg aatctggtat
     8101 cgtatgagtg gtacggaaaa agctcactac tgtttcacca aagtctattt ctgtatgctc
     8161 atctaccaca tggaaatcct taaatccttt ggctgcttca ttacgactga cattcaattt
     8221 tgctaactcg atcgtcaact ctgtcccgaa tactggtaca gaaatttttg acaacaagta
     8281 aggaagtgcc ccgatcgcat ctgcatgtcc atgtgttaaa aagacaccag ctacacgatc
     8341 aatgttttct tctaaatacg taaaatcagg gattacgacg tcgatcccca acaactcatt
     8401 ttcaggatat tttagcccac aatctaaaat aaaaatatcc tcgcccactt ctgcgacata
     8461 catgtttttt ccattttcac gaacgccgcc taatgggata attttaatgg tactcacaaa
     8521 cttcacctct tcttatgaca tccaagttta aagatgtcga ttttcttctt tatctaaata
     8581 tatgagccat tccaatgaag catatgcaaa atcactattt ctcaacttat ggagaatcag
     8641 caggaaaatt accagcacat ccgcgttctg acagttggat gggcatttta ttttggatat
     8701 acttcattaa caaaatggat cttttgcgaa acgctatagc cagaatactt atataagtat
     8761 tcagctgttc cccattatta tacaacaaat aaactaaatc gctagtatca gcaattgtta
     8821 gaacaactat gcacgtaaaa atccctctgt agcatctagg atttcttttg ctattcctct
     8881 ttcatttctt cttcgtcggt ttgaaacata cttttttcat tttaccacat tttttgcaat
     8941 aactatagtg actttttata tatacagaac ctcatgttga tggtgtgcaa acagcataag
     9001 atttaagcta gctttaagat aggttgtcta ttatcaaatg catctactag gagggatgca
     9061 aaatatgaat tttatgaaac gtgcatggaa aagtacaaaa gtaaaatggg gacgttcatt
     9121 gttattgttt gccgtgttca cagccattct tatttttgtg ctagctggcc tcacgattcg
     9181 aagcgctgca gaagaagcag cgaatcaagc acaaaaagaa gttggagcaa cagtgacttt
     9241 aagtgccaat cgagaagctt catttaaaaa acaggaacaa acttcttcaa gcggttctac
     9301 cactgaagaa cgtccagatc caggcagttt ttctcttaca ccagtttcct taacggacgc
     9361 ggaaaaaata gctgaattag ataatgtaaa gagttattcc tttgaaagtt ccgcctcagc
     9421 tatggcaaaa gatggaataa ctgccatcac tagttctgat tcaagtgaag acagttcaga
     9481 aactactgat gcaggcggtg aacctgctgg aggaatgggc gggaatccgc aaatgactca
     9541 agcagacttt caaatcagtg gtgtgtcagc aactgcacaa aatagtaatt tttctgatgg
     9601 aacagcaaaa atcacggatg gtgaaggaat agatgacagc gacaaaggca caaacaatgt
     9661 agtcattgat tctactctag cagaaacaaa tgacctggca gtcggagata ctttcacaat
     9721 cacgagtact gaagatgaag ataccactta tgaaatgacg atcaaaggaa tttatgaatc
     9781 cagcgaaaca agcggcagta tgggtatgaa attcaacttc atgaatccgt cgaatacact
     9841 ttacacttct tacacttttg caaatgaact aaatgggaca tctgacgata acacgataga
     9901 ttctgctatt tacactctat ctgatccaga taaaatggat gagtttctca aagaagctga
     9961 gaagctgatc gacacagata catttagctt acaatcaaat gattccatgt atcagtcaat
    10021 gttggaacct ttaaacaacg tggcaagttt ttctaaaaat gttgttttac tagtggctgt
    10081 agccggtatt atcatactta ctctgatcat tatgattacc attcgggagc gtcggcatga
    10141 gttaggcgtc ttgctatctt taggagaatc cagaagtaaa atcattctcc aattgtttac
    10201 cgaagtggct atttgtatga ttcttgcatt aggagttgct tcgcttagcg gaaacgtcgt
    10261 cgcgaatgcc gtgggacagc agctacttga ccaacagaca gaaaccacga cacaaaacca
    10321 gcaagctcct ggcggtggac aaatgccagg taacagacaa gaacaaggcg gtcaacgcga
    10381 tggtcaacaa ggaaccacga ataatccatt tgctgtttct gatcaagtca gcgaattgga
    10441 aatcaatgtc aaacctgcac aattgggact attagccgga gtagcatttg gaatcagtct
    10501 gttttctgtc tttttagctt ctatagggat attaagatta aacccaagaa aaatcctatt
    10561 aaactaaagg aggaatttga tgttacggac agaacaattg ggattttggt atcaagatcc
    10621 gcaagatgcg ctttataaag atgtagaact gaatttcgaa gctggaaaaa tgtacgctat
    10681 tttaggagca agtggttctg gaaaaacgac ttttctttcg cttatttctg gactagatgc
    10741 accaaaagaa ggaaaggtat actatcaaga tcgttcactg aacaaaatcg ggttaagaag
    10801 ttatcgtaaa aatgatgtct cgattatttt ccaagcttac aacttgcttc cttatttatc
    10861 ggctctagac aacgtcatag cagcaatgga aatcactaag accaagcaag agaattatcg
    10921 acaagttgct ttagatcgac tggctcgtgt aggaattgat gaaatattag caaaaaaaca
    10981 ggtgaccaaa ctttcgggag gtcagcagca gcgagtcgct atcgttcgtg ctatctgctg
    11041 tgaacatcaa ctaatcgtag cggatgagcc tactggaaat ctagatgaaa caacttcaca
    11101 agatatcgtc aaacttttcc aagaaatcgc ccatgaacaa aatcgctgta ttatcatcgt
    11161 gactcatgag attgaagtag caaaaagctg tgatgaagtc tttgaactaa aaaataaaaa
    11221 atttcaaaaa atcgaagttt aaaaaacagg ataatgatca aataaaaaat ccgaaccata
    11281 tctgtctcgc agatatggtt cggatttttt atttgcttga ttcaattagt aataagtggt
    11341 ttttattttt tttcgtcttt tcgagaaaac ttgtctttga cttcgtcgcc taaatttcga
    11401 gctttttctt tgatgtcagc acctagtgat tgtgctttcc cttctgtttc tttgctcttg
    11461 tcgtctgttg ctttgccgta tgcttctttt ccttcaccga ctactttatc tttcatatct
    11521 ttagaacgtc cttttaaatc tgccattgtg cttcctcctc ttagtttgtt tattgatcta
    11581 ccttcagggt aacatcaaca acagatacag acaaacagaa cgctcgtttt ctaaaaaaga
    11641 tagttagagc tgttgattaa aaactttttt cgaaagtccc ccatactttg acttcttttt
    11701 cttggaccat tatttctcct ttagcgataa ctgtatcaat atcaagcgtt tctttatcca
    11761 agatacacag atctgcatca gcaccaattt cgatttttcc ttttttatcc aatttcaata
    11821 tacgtgctgg attactggtt atagctcgta aggcgatttc caaagggata ctttcttttt
    11881 gcacagcttc tttgatccct actagcaatg ccttggcact tcctactcct aaccctagga
    11941 aatgattgtt ttcatcaaaa taaggcaagc ttccttgtcc atctgaggtc atcgtgaaat
    12001 tatctaaaga aacatcttcc tcgatcaatc gtttcagtgc tttgctgaag cgaacttcac
    12061 catctgtctt ctcccaaaaa tcaggatctt cgctagcagt gaagtcaatc gtgccaccac
    12121 gtttggcaaa tgccacgcaa gcttcaaata attctggtgt gcgatttgca tgagtaggca
    12181 aaaattgtgt gatcggaatt tctgtttctt ctacgattcg tgtcaatagc tctagctttc
    12241 tttttcctcc gcctaagtgg acattgatga tcccagcttt tccggataac atgcctccta
    12301 cacgagcatc tgctactgca cgagcgaact cttcaaaact tggctgagaa gaacggtgat
    12361 cggatactgc gatttctccg attccgataa ttttatcaat tgtcaaaaag tctttgatga
    12421 tagaatctgt tactgttttt accggcaatc ggtacgaacc ttcgtataca taagtggaga
    12481 ttccttctgc ttccagacct ttcgctttgc tgatcaacgc agtcatatct ctaccttctc
    12541 cgtctgttcc caaacaaccg acaactgtcg ttacaccagc tgtagtcaat tggctcaatg
    12601 tgacttctgg cgttcgattt tgataaccgc cttctcctcc tccacctaaa atatggaaat
    12661 gacagtcaat gaaacccggg gttagtactt ttccctcacc tgaaagttct tctacttcca
    12721 ctccttcgta gccgccttct agctgttctt ctacagccaa aatcttccca ccggcaatca
    12781 gtacatccat ttttcctaag taatctggtg caaacacttc aatttgtcga atgattttca
    12841 tacatttttc ctctttcttt aagtataact gatgatagga tcttaaaaaa taatccaaac
    12901 gataggatct ttactccttt gattttaccg taaaatgacg gttcttggag tccttatcta
    12961 ctgtttggat tactttttta tttacaggtc tccagtacta ctgcagacct attgcaatgg
    13021 caatcatgat gaaaatagtt cctaaaacaa acaggaatcc ttgcatcttg atttggaatc
    13081 ttgcccattt tgcccattcg atccgtgcaa cacctagcac accaatcaag ctggctgata
    13141 cgggtgtaaa agcgtccata aaacctgaac caagctgata agcaagaact gctacttgtc
    13201 tggatacacc tactaaatct gccagtggag ccatgattgg catagtcaaa gctgcttgtc
    13261 ctgaatttga tgtaacaacc aagttgaaca aactttggaa gatgtacatc gcccaagcac
    13321 caatgacagc tggaacaccg gtcaacgccg tcccgatccc atgcaaaatc gtattcaaag
    13381 ctgaaggaac agatgcatca gatcctccca agacaagaag aatccctttg gccataccta
    13441 ctacgattgc tgtaccagct aaatcagctg caccagattg gaaagaagaa gcgatgtcat
    13501 tgactttcat tccatctaat ttgaaaagaa cagcaatgat tccagcaact aatcccatca
    13561 caaagaattg agaagcgatt tctggaatgt aatagccttt ttgagtcacg ccccaaacga
    13621 tccagacaag gacaactaac atctctaata agatcaattt atgcccaagc atgaattttt
    13681 tattttcatc agatgtcttt ttgatatgag aacggaaata atcatctgat ttgtaagtca
    13741 acgaactttc tggtttttta cggatttttt ctgcatatat catcatataa ccagcggcta
    13801 atgcagtaac aactacccac ataatcaatc ggaatgtcgc accagacaac acggggattc
    13861 cagcaatccc ttgagccacc gctacgctaa acgggctcat ccaagaggtc gcatttccga
    13921 cttgagatgc gacataagtc acggttactg ctacgatcga gtcatatcct aacgcaatga
    13981 caaatggaac catcaccatt gagaatggaa ttacctcttc tgccatcccg aatgtagcgc
    14041 caccgaatga aaaagcaaag aataatagcg gtaaagctaa acgctccagt cccttagttt
    14101 tactgataaa cgcatagata cctgcgtcga tcgctccggt acgcataata ataccaaaag
    14161 cacctccaac aacaaggatc aatgcagcga tccctacagc agatccatac ttatctccag
    14221 atactagtcc ttcaaaaaca aaatttaaga aaccaaagcc gccaaaatca tctgttcccc
    14281 atattttggc tgttttatgc aattttttag aagtatcata gatattttcg ccgtactgat
    14341 cgtaaagcac atcatctgtc aaggagattt catctagttt ttcctgcgta agattttttt
    14401 ctccgtcagc aatgactttt tccaatcctt tttccggaac atccagtttt tcacgttctg
    14461 ctggatgatc ttgcagttct tctaattgat caaacacata cgacttatcc agctcgtaag
    14521 cataacggaa ggaatcttga cgtaaaacag tacgagtact tgtttcgcca tttgcatctt
    14581 tgtattcaat atcttgcgtg ctgaactttc ctgctggaac gacaaaagtc agtatccatg
    14641 caaagatgac tacaccaaaa ataatgacat acgtatgggg tgtcgataat ttagtgaagt
    14701 cgcgtttcgg ttttcctgaa gaatttgttt tcttcaaatt tatttcttcc tttctttttt
    14761 cagtaatttt gatgaataaa aaatggataa tcaaatgaat tatacagtaa aaatgacatt
    14821 tccacatgaa taatttatta gaaaatcact tttttctatt ttgttttttt tctcctatgt
    14881 atgattattt ttttctcttt ccatttatat taagtatttt atttctctgt ttttccttaa
    14941 aattcttttt ttatttttag attagagaaa atctcgtcct gtttttttca ttttacaatc
    15001 attgaaaaat tgaatttcat tgcaaaaaaa ataataaaca cttataaata tccattatct
    15061 atccttcaat ttttgtgtat tttatttcta ttagaaaaag gtatcctgtt ggacaatttt
    15121 gttatatcag gtaaacttca ttgcaataat gataacaaat gtactataat tactttgtaa
    15181 agagttttgg ggaagactct ttcatcaacg tgtattaaga gacaagaaag aatccatatg
    15241 gattctttct tgtctctttt tccactactt tttatttttc tgacactaca tattcttata
    15301 actcatttct cataactgtg aaacaactta taaaaataac aaactttaaa caaaacaagc
    15361 ttaaaatcaa agatgatcca tcttaaaata actaaaacaa aaaaagtagt cgttgttaat
    15421 cctcattaat aatcgttttc tttttttcaa acgctccatt tttatgtgga acattatttt
    15481 tttaaggaac tttgtcaaca tgtggataac tattttccac ttgtgtcctg gactagctgt
    15541 tttgctgtaa aatacaaaac ttgctgcagc aaaaagatcg ttgcaagaga ggctgttccg
    15601 cagatcgctg gatctagatt tggcaaagtc aagactgcat catgtaaatt ggcgtggttg
    15661 tccaaaacca aatcagcaaa ttcaaataat cgcaaaccac tgctatgttg cgatttgata
    15721 cttcgtgaca aatcaaatcc agtaacaatg attaatggat gaccattttc tttgatccac
    15781 tgagctagtt cgatgattgc cgggtttctt ccttcattcg aaagaagaag aaaaatgtct
    15841 tctgatttga cagaaagctg atcaataatt gcctgtcctg caccttctag cgtttcatag
    15901 acacctccgg ctggattttc aatcaatata cttggtacaa gatccggggt ttgatcccaa
    15961 aattcaaatg ctacaccact tagtgtgcgg ctagcatata tctgcaatct accaccttga
    16021 ttaatttgtt tcgcgatcca agtgccagct tgaagcatca gcggctcttc tatccttaat
    16081 ttctcttcca attggtgata atagtcaaaa taacataaac tcatttatct ctcccgccta
    16141 gctatacttg acatgaatag aaacagctgc ctaatctaag gaagtacata aaaaaacagt
    16201 agttagacag taacacatgt ggtctttgct gtttcaaaca caaaaaaggc attgaacgaa
    16261 tacgcccaat accttgacat agcaaataaa acaacgtgca gaccatggaa aatatctaca
    16321 taggggtaga attttcaccc tagattaggt acaataaatt aggtcgatgc actgcattga
    16381 ctatggcaaa atgatcggtc agggtgtatg tgtgtgactg gtcattccct gattcttagc
    16441 gccaactaag ggtcagggag ccttttttta ttcagctgtt tctatctgaa tttattgtaa
    16501 gtaatctctt atccaagtgc aagcaagaaa atacaaaaaa acattggtat gatttatata
    16561 tatataaata acataaaaaa aggaatggct tatcccgata caacaagcat tcctttaaaa
    16621 aagattcttt tatataagtg aaaaaagctt ttttccacca attaaataaa tctctcatct
    16681 caatagttta tccattattt tatctttttt tgcgtatacc tactaaatat taccgtcttc
    16741 ttctcgttct tctactttgt tcgagagaat ctgtactaga aaaagtagtt gtatgtctac
    16801 cttttctgcc tatccgagac tttcttttca tatatgggat ataaatgatt agatacagta
    16861 cagcaaaaaa taaagcaaat acgcaagctt cccaactacc taacgccaat tcaaccatca
    16921 aggtagcaac cccaacggat gctgcatacc agataaaaat cagaaacgtt tctttttttc
    16981 tgaccatgcg aaacatgata aatagactaa taagcaatag tggcaacaca aatatccaat
    17041 tgcttccttc catcagccac ttgagtatat caagcagcga ctgtgcaaca gcgactaatt
    17101 ttcccatagt gaacacacac ctttttttat aattaaaaaa ttcgcctttt tattctacta
    17161 tgaatcttct ggcttggctt tcatttctct tatgtaatca ttttctttta tacaacttaa
    17221 aaaaaaatta aaccattagc agaatatttt ctgaatctat tcacgaaagt ctttttctat
    17281 ctttcgtttt ccacgactca actgatctag agtcaaaaaa ctaaaacgac agttgcgcaa
    17341 atgacgcaca agctgccgct ttaataacaa atagcctttc attctcagca ggccacttat
    17401 ttccagttaa ctttctgttc ttagcttttt actcaactgg ataatatatg cacttaatga
    17461 agtcccaatt tccgaatgtt gaagtccata ttcaatattt gtcttcatat atcctaactt
    17521 atcgcctacg tcgtatcttt ttcctttaaa ctctctcgca tagaccgcct ctgtctggtt
    17581 aagagtctga agtgcatccg ttagctgtat ctcgtttcct gctcctggtt cttgagtttc
    17641 taatatctca aatattttag gagtcagtag ataacgtccg ataatggcta aattacttgg
    17701 tgcttttccc atttcaggtt tttctacaaa cccttttact ctgcaacgac ccttttcgct
    17761 atctgtttta ggatcaataa ttccgtattt agatgtttca ttttctggta cacgcatgac
    17821 ggcaacagcg gaagcttgtg tttcttcata atccatcatc aattgttgag tcaatgggat
    17881 atcatctttc atcaaatcat cccccagcat gacgacaaac ggttcatttc ctataaatgc
    17941 ttttgcttga agcacggcat cccccagtcc tcttggatga gattgacgga tgaagtgaag
    18001 attgacttga gtcgtttctt caactaaagc gagtaattct gttttttcct tttcccgcaa
    18061 attgttttcc aattcgatat ttgcatcaaa gtgatcttca atgggtcttt tttcttttcc
    18121 cgttacgatc aagatgtctt cgattcccga atccaatgct tcttccacaa taaactggat
    18181 cgttggttta tccacgattg gaagcatttc ttttgccatg gcttttgttg ccggcaaaaa
    18241 acgtgttcct agcccagcgg caggaatgac tgcttttttg accttcactt gct
//
LOCUS       DAMYXM010000050        18107 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.051, whole genome shotgun sequence.
ACCESSION   DAMYXM010000050 DAMYXM010000000
VERSION     DAMYXM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.051"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            14..1807
                     /gene="uidA"
                     /locus_tag="PNS21_001955"
     CDS             14..1807
                     /gene="uidA"
                     /locus_tag="PNS21_001955"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDI5159660.1"
                     /translation="MTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESYPISVP
                     SSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNGKLINQ
                     HKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKKENLVN
                     FDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILIAIYDE
                     EAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGIRSIQV
                     SNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTSHYPYS
                     EEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKMAIKEL
                     IERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYLKSTPD
                     ICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTEYGADT
                     VAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILRVQGNK
                     KGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            1979..2380
                     /locus_tag="PNS21_001956"
     CDS             1979..2380
                     /locus_tag="PNS21_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDI5159661.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            2374..2619
                     /locus_tag="PNS21_001957"
     CDS             2374..2619
                     /locus_tag="PNS21_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5159662.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(2943..3164)
                     /locus_tag="PNS21_001958"
     CDS             complement(2943..3164)
                     /locus_tag="PNS21_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159663.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(3326..3398)
                     /locus_tag="PNS21_001959"
     tRNA            complement(3326..3398)
                     /locus_tag="PNS21_001959"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3363..3365),aa:Lys,seq:ctt)
     gene            3474..3704
                     /locus_tag="PNS21_001960"
     CDS             3474..3704
                     /locus_tag="PNS21_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5159664.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            3748..4917
                     /locus_tag="PNS21_001961"
     CDS             3748..4917
                     /locus_tag="PNS21_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDI5159665.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            5491..6060
                     /locus_tag="PNS21_001962"
     CDS             5491..6060
                     /locus_tag="PNS21_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDI5159666.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            6047..6907
                     /locus_tag="PNS21_001963"
     CDS             6047..6907
                     /locus_tag="PNS21_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDI5159667.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            7097..7801
                     /locus_tag="PNS21_001964"
     CDS             7097..7801
                     /locus_tag="PNS21_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI5159668.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            7806..9641
                     /gene="walK"
                     /locus_tag="PNS21_001965"
     CDS             7806..9641
                     /gene="walK"
                     /locus_tag="PNS21_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDI5159669.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            9638..10954
                     /locus_tag="PNS21_001966"
     CDS             9638..10954
                     /locus_tag="PNS21_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159670.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            10955..11824
                     /locus_tag="PNS21_001967"
     CDS             10955..11824
                     /locus_tag="PNS21_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDI5159671.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            11879..12688
                     /locus_tag="PNS21_001968"
     CDS             11879..12688
                     /locus_tag="PNS21_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDI5159672.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(12791..13420)
                     /locus_tag="PNS21_001969"
     CDS             complement(12791..13420)
                     /locus_tag="PNS21_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDI5159673.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            13590..14882
                     /locus_tag="PNS21_001970"
     CDS             13590..14882
                     /locus_tag="PNS21_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDI5159674.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(14958..15620)
                     /locus_tag="PNS21_001971"
     CDS             complement(14958..15620)
                     /locus_tag="PNS21_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDI5159675.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            15845..16129
                     /gene="groES"
                     /locus_tag="PNS21_001972"
     CDS             15845..16129
                     /gene="groES"
                     /locus_tag="PNS21_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDI5159676.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            16180..17805
                     /gene="groL"
                     /locus_tag="PNS21_001973"
     CDS             16180..17805
                     /gene="groL"
                     /locus_tag="PNS21_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDI5159677.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         6259 a         2901 c         3720 g         5227 t
ORIGIN      
        1 gttatatccc attatgacag agacgaggca agtaattgat ttaagcggtc tttggagatt
       61 taagttagat acaaatgatg acttacaaga agaacttgct ttagaaccaa tggttgaaaa
      121 agagagttat ccaatttcag ttccttcttc ttataatgat ttatttgaaa cagagaacat
      181 tagaaatcat gttggatggg tgtggtatga aaaagaagtg attcttccta aaaaattatt
      241 agaagaaaga ttagttcttc gttttggatc tgcaacacat gaagcaaaag tatttttgaa
      301 tggaaaattg attaatcaac acaaaggagg gtttacgcca tttgaatcag aaattaatca
      361 gtttataaaa gatggaagta acagaattac agtggctgta aacaatgtta ttgacgatag
      421 cactttacct gtagggattt tgaaagaaac gatacaggaa gatggagaca taaaaaaaga
      481 aaatttggtt aattttgact ttttcaatta tgcaggcatt aatagacccg ttaaattgta
      541 tacaactccg aaagactata taaaggatgt cactattaat acaaaaataa ttgattcaga
      601 tgcaaatgta gaatttaata tcgatacaga atggaccgga gaaattctaa tagcaatcta
      661 tgacgaagaa gctcaactta tagcagaagc taaggggaaa gaaggagtga ttcaacttaa
      721 gaacccaaca ctttgggagc ctctaaatgc ttacttatat agtatgaaag ttagtttaat
      781 taacgaaaat aaagtgattg atacgtacac agaagaattt ggcattcgta gtattcaggt
      841 ttcgaatggg aagtttttga ttaataataa acctttttac tttaagggat ttgggaagca
      901 tgaggacacg tatattaatg gtcgtggttt gaatgaagca gtaaatatca aagattttaa
      961 tttaatgaaa tggatcggtg ctaattcatt taggacttca cattatcctt attcagaaga
     1021 aattatgcgc ttagcagatc gagaaggtat tgtagttatt gatgaagtac ctgcagtagg
     1081 attacatttg aatttcatgg ctactttatt agatgacaac gtagaaaaac atgatacttg
     1141 gaaagaaata cggacagaaa atgctcacaa aatggcgatt aaggaattga tagaacgcga
     1201 caagaaccat ccgagtgtcg taatgtggtc tattgcaaat gaaccagatt cggattctga
     1261 aggcgctaaa gaatattttg aacctttagt tgaattgact aaagaacttg accctcagaa
     1321 gagacctgta acaatcgtta cttacttaaa gtcaacacca gatatttgta aagtaggaga
     1381 tattgtcgat gttttatgtt taaatcgcta ttatggttgg tatgtttcag gtggagacct
     1441 aaaacatgca aaatatctgt tgaaaaaaga gctagatgga tggatggaaa gatgtcccga
     1501 tacgccaata atgtttacag aatatggagc agatacagta gctggacttc atgatacagt
     1561 accaacaatg tttacagaag agtatcaagt gggttattac gaagcaaatc acgaagtaat
     1621 agataaatgt gacaattttg ttggtgaaca agtgtggaat tttgcagatt ttgaaacaag
     1681 tcaagggatt ttaagagtgc aaggaaataa gaaaggaatc tttactagag atagaaagcc
     1741 taaaatgatt gctcactatc taaagaaacg atggtcatca ataccaaatt tcagttataa
     1801 aaaataattt accttttata aggaatataa aaaaggagtg gctgcttaat tgccaactcc
     1861 ttttttttat aaaaagattt tctgtttaaa gaaggatatt tttttctagt tttttcgaaa
     1921 aaaaggcgct ttctgattgt tgtattagtg gaaaggggaa aaaaggagca tatttatcat
     1981 gcaacaaaat tacaatgaga aatctataca acatcaattt gatgcctact gtaaaaaagt
     2041 attacgaaac gaaactaagt ctattcgaaa acgaaacatt aacataacag ataatgagga
     2101 accattgacg aattcaaatg aaggtaagta ctcccaaact cattttgatg aacaagatgt
     2161 ctatttctta tttggaatgg aaatattaat ttcagatcaa aagttatcgg cagccattga
     2221 tcatttatcg gaaataagaa gaaaggttat cctactttat tattttgcag gttttaatga
     2281 tactgaaatc ggtaaaatcc tcaatatgag tacaagtggt atctggtatc aacgaaagaa
     2341 ggcagttgaa caattaaaaa tggggtatga cttatggtag acaaccccaa aaaacggcag
     2401 tatccattag ttcctctatc cattatccaa tctgctctag aagaagatcc aatttcaatc
     2461 aagatgatac tcaaacatta tcatagttat atagctaagt tatgtttgac aaatggattt
     2521 aatgaggcgg ggcaatttat tacctatgta gatgagtata tgctaagaca gctagaaatt
     2581 aaattgatag aagcgatctt aaaatttaaa attaactaac taaaaagtcc cctttccact
     2641 tattctgttt aaaagaatct tctaatagtt aaaggattag tagattcttt taaacagaaa
     2701 acctattgat cattgaaatt taaatacatt ctatcagtac gttaacactt caaggagcat
     2761 attttagttg cgccatgatt tcttactgta aagaaggagc gataaacatt ctaaaataat
     2821 ttttatagtc aataaaaagc gatgatttga agttgggata atgatacttc cgtaaggctc
     2881 gccgtaaact tggagagagg tgaaattcct atgtgctttt gacaaaagca gctgattaag
     2941 aattatttcc atatgttgat ttcaacattt tttctattat ttttaagacc aaattcagtg
     3001 aggcaaatag catcatgtaa atttagtttt tctattgaac gttctccact ttttagtttt
     3061 tttatagtac tcaaatttaa tcctgtatgt ttagagatct catttgcagt atggttttgt
     3121 aaagtttctc ggattattgt tatattagca atttcattac gcataattat gttcctttct
     3181 tatcaaaaat atcaatgact acagtgggct tttagtgaaa aaaaggggta ctacagtctc
     3241 ttttgaaaac aaaaaaatac agtatatacg ttttctaaat gctaagcacc aacgtttaca
     3301 ctgtatttaa gggtttggca cccaatgggc tgtgaggggc tcgaacccgc gacccgatga
     3361 ttaagagtca tctgctctac caactgagct aacagcccaa attaatactt tgttattcta
     3421 acacctaaaa taaaaaagtg caatacttat tatcagaatt gagagtgaat tttatggatt
     3481 ttcaagatta tgaaaaaata tttgaaagtt atccatcgat attgaaaatt actgatttaa
     3541 ctaaaatgtt aaatattgga aaaaataaag cttataattt gctgaaagat agatcaatag
     3601 aaaatataaa aattggagca aggtacaaaa tacctaaaaa agctgtcatt gagtatatat
     3661 tgaaaataca agatgaaggt aaaaatcatg ctataataaa atagtcaaaa tttttgccga
     3721 tatttcagtt taagaggaga gattgaattg aaattaagag caaacataat tgaaaaaaga
     3781 ggaacattac aagtagttgt atcttataaa gactctaacg gaaaaattag acaaaagtgg
     3841 aaagatacaa aattaaaaaa gaaaggaaat aaaaaacgag cagaaaaaat tagagatgaa
     3901 tttttactag aattggaaag tcaattagct atggaatgtg aatgtgctaa tccaaattta
     3961 ttgttttatg actatttgct taattatttg gaagtatcaa aaaaacaagt agcatttaac
     4021 acatatgttg ggtataagca ttacgtccat aatcgaattt acaagttttt ttatcctaaa
     4081 aaaattaaac taaataggtt aaagccttat catttacaag agttttatca atatatgtta
     4141 aatgataact gtacagcaaa tacggttatt cattatcacg catttatacg caaagctctt
     4201 caagaggctg ttattacaga attgataagt acaaatgtag cagataaagt aagaaagcca
     4261 aaaaagaaac aatttgtttc tcaagtttat aatcaacaag agatcttgaa attacttgac
     4321 attttgtcag aagaaaaact ctatttggta gttcttttaa cagcatttta tggattgcga
     4381 agaagtgaag ttttaggatt aaaatggtct gctattgatt ttttaaacaa aaaaatcatg
     4441 ataaaccatg taatcataga aaatccagag aatatgtcac agttaataaa gaaagatcaa
     4501 acaaagaact cttccagcta tcgtacatta cctttggttg aaacgattga acgagcatta
     4561 gtaaatcaag ctaaatggca acaagataat cgtattcttt taggagatga ttatatttta
     4621 gaagataatg agtacgtatt tactatggaa gatggacggt tgatgaagcc tcaatatgta
     4681 acgcatcgtt tgagtaagtt gattaagaaa aatggattga aaaaaattcg ctttcatgac
     4741 ttgcgtcatt ctaatgcttc aataatgtta gatagtggcc agaatatgaa atccattcaa
     4801 gaatggctgg gacatgcatc gtattcgaca actgctaatt tatacacgca tttaaccgca
     4861 ggagtaaaag atcgtgcagc agaagcatta gaaaacgcat ttgggtttaa agaataaaaa
     4921 aacaaaaaac aatatctttg gggtatcgct tcttgttttt agaatttttt tgttggcaat
     4981 ttgttggcag tatgttggca atgaaaaaat tcacaaacta aaaacctatt tataaagctt
     5041 tataaaactt ttttcacaaa tagagcattt ggttaagagt cagctgctct accaactgag
     5101 ctaacggccc gtacaaaaag tacttaataa tagtagcact aagaatttta aaatgcaaga
     5161 ctacatttaa agaaaatcag aaaaatcttt acttccttat aggaaatcat tcatagatta
     5221 ttcacatttg ctcgctatta tataagcata ccaacaaaca aagttcttgc aagagaacaa
     5281 accctaacac ttttttcatt ttttactcct ttcccgccgt ctccccggcg ggtctcctgt
     5341 tcttaacgat aaaagacaag aaggcttagc cttcttgttt tttgttttca tggaatcccg
     5401 ctaatcaaat cacctagtta aagcaactag attgaatgtt tatcataagt ctgatatcat
     5461 gtttatgaaa tgaaaaaagg agcggttatg atggaaaaaa tgcgtcagca attaatggag
     5521 tgggggaagg aactagaaac attccgtctg cctcactggg aagaactccc tgacttagaa
     5581 ctttatatgg accaagtcag aacgttagtt gatcgttatc tttcacctgt gatacaagga
     5641 gaaaagcatc cattgcttac ctcttctatg gtaaacaatt acgttaaact aggtttgata
     5701 ccagcaccag ttaaaaaaag atataacaaa gaacacgtag catttttact tgcaatcact
     5761 acgttgaaac aagtattgac tattcctgaa atcaaagaag ggatattgtt ccaaggaaaa
     5821 acagtaggaa ttcgtgaagc ctataatcta ttttgcgatg aacaagaagc agctgtgtgg
     5881 atggtcagcc aattagcaca aggtaaatct catccgcaaa aattcgacca ggcaacccct
     5941 gtagaatata ttgctgtaag agcggcaact ttatcgtttg caatgaaact tcttgcggaa
     6001 aaaacgattg ttctagaaac cgaatattta aaagaggaga aaacaaatga aaaacaataa
     6061 aatcgcaatt ttagtagatt caggtacgga tgtgccagaa gaattgataa aaaaatacga
     6121 gatgtatatg atcccattga aaattattta taaagatcag gtatatacag ataaaattga
     6181 cattacctct gaagagatct atcaacgttt gccagaggaa atccctggta catctttgcc
     6241 ggatggtgag tcgatcacag aaatctttga gcaaatcaaa aaagacggtt atgaaaaggt
     6301 acttgcggta acaatttcca gtggattgag cgggacatat aatgtggttc gtttaattgc
     6361 acaacagcaa gaagaattgg aagcatatgt actagatacg aaaaatattg gtattggtgc
     6421 aggtttttct gcgatccaag cagcaaaatg gctggaagaa ggagtagaat gggatcaact
     6481 gatcagtaat ctgaatgagt tagtcgaaag aacaaaagtt ttctttaatg ttgcgacatt
     6541 ggaatatctt caaaaaggtg gacggatcgg tttagttgct tctattgtag gaaccgcttt
     6601 aaagcttaac ccaatcatct catgtaatga agaagggatt tattacacag taggaaaagc
     6661 taggggacgt aaaaaaagtt tggataaaac ggttgcttac gtcaaagaaa aaatcggtaa
     6721 tgcaaaggtc ttcaacttag ctgttgccca tggggatgcg aaagaagaag caattgaaat
     6781 ggaagcgaga cttaaaagag agttcccaca agcaaaccaa atctactttg gccagatttc
     6841 tccggcatta gtagtccaca ctggtccggg attattgggt gtcggtgtac aagttttaga
     6901 aggataaatc aaaaaaagga aacactgagg tgaaaatcag tgttttcctt ttttattgaa
     6961 gttagaacaa aaaataataa agtattttgc actttcattt taattttctc ttttgtttta
     7021 taggcaaaga tgaaacaaaa gcagaaatac ggtataatga gaaggtatga caataatata
     7081 taagaggtgg ctctttatga aaaaagtttt agttgtcgat gatgagaagc cgatttcgga
     7141 tatcgtcaaa ttcaatttag ccaaagaagg ctatgatgtt tatactgcat atgatgggga
     7201 agaagccttg gaaaaagttg ctgaggtaga acctgatttg attttacttg atttgatgct
     7261 acctaaaatg gacggcttag aagttgctcg tgaggtacgc aaaacttatg atatgccgat
     7321 cattatggta acagccaaag attctgaaat tgataaagtc ctaggcttag agttaggcgc
     7381 cgatgattac gtgaccaagc cattttccaa ccgagaatta gttgcacgtg tcaaagcaaa
     7441 tcttcgtcgc ggtgccacgg ctgcgaagga accagaagag gcagcacctg cggaattgac
     7501 gattggtgat ttgacgatcc atccagaagc ttacatggtg acgaaacgag gagaaacaat
     7561 cgagttaaca catcgtgaat tcgaactact cttttacttg gcgaagcacc ttggacaagt
     7621 gatgacaaga gaacatttat tacaaacggt ttggggttat gattattttg gagatgtacg
     7681 aacagtggat gtaactgtac gtcgtttgcg tgaaaagatc gaagataatc caagccatcc
     7741 aaattattta gtgacacgtc gtggtgtcgg ttactatttg cgtaacccag aacaggagta
     7801 attggatgaa aagaaaagtc cgcttttttc gatcagtcaa ttttaagatc gctattacct
     7861 ttattctgat tttattgatc tcgatcgaaa ttattggtgc ctactttata cgtggtttag
     7921 aacgttccac gatcaataca tttattaaag atatgaatca aacggtggaa tcattagcaa
     7981 caacaatcag cccggaattg aatcgaaaag ataatgcaga tgatgaagag gtaaatgcga
     8041 atatcaaaag atttattgaa aacagtgcga ctagcgatat catcgaaata cgggtcgttg
     8101 acgaaaaagg gatcatccga ggaacaaccg atgtcaatga acaaagcgct gtgggaaaga
     8161 aaaatgatta tgttgatatc aatgatttca cgactaaacg ttatgttgct ttagataacg
     8221 acgataaacg ggtgaatatc aatgttcaac cgatcctgtc cccgaccggc gatgccgtta
     8281 tcggggctct ttacgtcaaa agtaatatcg aacaaaaata ttcggaaatc aataacacag
     8341 cagtcatttt ctttactgcc tcactaattg cggcatttat ttcaatgatc gtatctgttt
     8401 tagttgctcg ttcgattaca cagccgatcg gtgaaatgag agaacaagcc attcgaatcg
     8461 ctagaggaga ctacagtcgg aaagtcaaag tgcatggaca agatgaactt ggacagctgg
     8521 ctgatacctt caatcagtta gctgaacgta ttgaagaagc tcaagataca atggaagctg
     8581 aacgcaatcg tttggatagc gtgctatccc atatgacaga cggcgtcatt gcgacagatc
     8641 gcaggggtaa agtcattacg atcaatgata tggctgtttc cttgctagat atcaaaaacg
     8701 aggaagcaat cggacaatcg attttgacac ttttagatat cgaagaagaa tatacgttgc
     8761 ggaaattatt agaagatcca aacgaaatgg tacttgaccg ttcaaccagt acattggaaa
     8821 gtgatcagat gatcttgcga gtagactttt ctatgatccg tcgcgaatca ggttttatca
     8881 gcggattagt agctgtcttg catgatgtga cagaacaaga aaaaacagaa cgggaacgtc
     8941 gtgaattcgt ctctaatgtt tcccatgaac ttcgtacacc gttgacaagt atgcgtagtt
     9001 atatcgaagc attgagcgaa ggtgcgtgga aagacgagga agtagcacca aattttttga
     9061 aggtaacgct agatgaaaca gaccgaatga ttcgaatgat caatgacctc ttaaatctat
     9121 ctcgcatgga tacaggtaat acccagcttc aattagaata tgtcaatttc aacgagttag
     9181 tgaattttgt tcttgaccgg tttgatatga tggtgggaaa tcaggaaaaa aattataaga
     9241 tccgtcgtga atttacgcaa agagatcttt gggtcgaagt agatacagat aaaatcatcc
     9301 aagtagtcga taatatcatg aataacgcga tcaagtattc accagatggg ggaacgatta
     9361 cttgtcgctt attagaaacg cataataacg tggttttaag tatcaccgat caaggattgg
     9421 ggattcctaa aaaagatctg aaccgagtgt tcgagcggtt ctatcgagta gataaagcac
     9481 gtgcaagagc acaaggtggg acaggacttg gattagctat ttctagagaa gtgattaagg
     9541 cgcatcgagg cgctatttgg gcagagagca aggaaggaaa agggtcgact ttttatatct
     9601 cgctgccata tgaaccttat gaggaggaat ggtgggaatg agaatctctg aaaaagtcgt
     9661 tcgtatagga ctcattttga tggttgcgtt aagcatatac ttctcttatg caatctggct
     9721 gagcccagca ggaaaaagtt caatcaacgt agatgagaca aacactcaaa tgatcgagtc
     9781 tcaaagcaat cgaaaaactt ccgaagtctt tcttccgctt catgttacat ggcaccattc
     9841 gggaatgatc caagaaacca acagtgaaaa tctcgtgtac cgattgcaat tgatcatcga
     9901 taaagcaaga tttggaaagc tttctgaagt agtttcagga gacaaatcac agtttgaaca
     9961 ggtgaaaaat ctgaactctg gttttgagtt ggcctataat gcgccttttt tgctaagaga
    10021 atacaaggaa gcatttgatt tagatttaga tgtgtcgaac ttggcaaaag aaagcgaaaa
    10081 tatgtatttt actagaatac aattcgatca aggaaataaa aaaatccgat ttttgaatta
    10141 ttcgaaccat ctcgtatatg aagcaaatat ttcgttgaat tggacagata tcaataaaga
    10201 attaacagca aaagacacaa aatggacaga aatgacaaca gaaccatcga cgatcgacac
    10261 gcaatatgct acgaaacatt cgatcaaact gaaaaagtat agttatatcc tgtcacagca
    10321 accctataca ctttttagaa atgctttttt ccagaaacca gatagcgtga aaaataatga
    10381 tgaaagcaat gaattgattt tttacggtgg gaatgaaaca atgtctatcc attcggaaac
    10441 gcaagtagtt gattttcgtg gagagctgcc agagaaaaaa gaaaatgcta gtgtattttc
    10501 tgaaagtttc ccctatatta gcaaattagg cagttcttta gggaacctca gatattttga
    10561 ccgcagtgac aatacgatcg attatcggat attcgtggaa ggcttcccgg tttttgggac
    10621 agacagcaga ggacagattg gtgtggaagt tggcggtgaa acgaatggtt ctgtgcaagt
    10681 gaatatccaa accagtttga atacgattca agtgccgata ccttcagacg aagaagtaga
    10741 acttgcaagt acgaatgaaa tcatccagca gttaaaagct aagggagcag atagcaacaa
    10801 gattggttca gtgatcgttg gctatacatg gcaaaatgtc aaagaaacga atcgtgtcgt
    10861 tgatctctta cctgaatggt atgtgaaata taaagataac tggtattcag ctaacgacct
    10921 tctggaaaaa ttacctggat tggaggcgaa ttaattggat ttcaaaaaaa tcgaatggat
    10981 atttttcgtc gcttttttag ggttgaatgt gtttttattc agcagttatc atgacgcggt
    11041 ataccaagaa aacaatgtca ttcgctctaa tcagactgaa tcaatccaac aacgtctggc
    11101 tagcgacgat atcacttaca cggggaagat ttcgtctgag aacaagcaag gctattatct
    11161 tagcgcggag cagaccaatc ttagcacttc attagcgaat tatcgcaagg ctggtaaaag
    11221 cggcttgctg gcaagtggtg tgtttttaga cggtgatgtt ttgacgaaat ctttatccag
    11281 ttcagatgct gaaaagaacg tgatcgatcc agataaaatt agtagttcct tggaagattt
    11341 tttatctaac aaagacaata ttttattcgg taatgattat gtgtacacga aaaacttttc
    11401 tcatttagaa gaagaaccac tagaaatcca agcaagccaa gagtatgaag gcatcccatt
    11461 caatgatgat acagcaaaaa tcgttctttc tttagaaaaa aacgataatg attatagcat
    11521 cacgaaatac atgcagacgc atttatctga tatcgagcag ctgcgcgaaa agacggaact
    11581 gtatacggaa gaggaagcaa tcaatacatt gtacatcaat aacaaaatac caagaggatc
    11641 aaaaatttta tggcgtcagc tagcttattc ctgtattctg aaagtccggg aaaaaaatgt
    11701 gtatgttcct gtttggcatg tagcgatcga aacaagcgat aaagtggtcc aaattgagag
    11761 tgtcaacgct ttcagcaata cgatcgtgac aaacaatact gtaccaaaag tggaagatca
    11821 ataaaaaagc gagtataatg aaaaaagttt acaaagtaag aagggaagta ggagtcaaat
    11881 ggctgaagaa aaaccagctt ttaaaatcag tatcctagca agtggaagtt cgggtaattc
    11941 actttatatc gaaagtgaaa aaaaacggct tttggtcgat gcaggactta gcggtaaaaa
    12001 aatcacttcc ttgatggctc aaattgatcg gacaccggac caattagatg gaatacttgt
    12061 gacgcatgaa catcgagatc atatacatgg cgtgggtgtc ttggctcgta agtatcattt
    12121 ggatgttttt gccaatcagc agacttggga agcgatggaa ccactgattg gaaatgtacc
    12181 agtggaacaa aaacatctat ttgagatggg gaaagtcaga acattcggcg atatcgatat
    12241 tgagagtttc ggcgtctccc atgacgcagc ggctccgcaa ttttatcgtt tccataaaga
    12301 aaatcggtca ttcgtcatgt tgacggacac cggatattgc agtgattata tgcgaggtgt
    12361 cattgaaaat gccgatggtt atttgatgga aagtaatcat gatcttgaga tgttacgcat
    12421 gggaccttat ccgtggaatt taaaacagcg tattttagga gatagaggac atctttccaa
    12481 cgaagacggt gcacttgtca tgactgatgt gatcggtaat cggaccaaac gaatctatct
    12541 tggccatctt agcaaggaaa acaatatgaa agaacttgct catttaacaa tggaaaatgt
    12601 tttgaaagaa aaagattttg gcgttggaca tgattttgaa atctatgata cagatccaga
    12661 tgaagcaact gagctatttt cgatttaaac agttcaatca ataaaaatat aaaaagcaga
    12721 agataaaatc aatcagggtt ggttttatct tctattttta tttttttagc atattaaaaa
    12781 aataaccgta ttatcgactg actacactga tgtgctgctt ttcatgtttc tcatccagta
    12841 tctcatctac taatgcaatc gcgtaatctg ccatgctgat cgtactgtcg ccagcagcgt
    12901 ttctttgtaa ataatcatca cttaacaaat aagtacctgt acgtttgcca tgtggatcaa
    12961 ataaagaagc tggactgata taagtccaat gtagattttt agattgtttc aattttaaca
    13021 atccttcgta catattgaaa gctgttggat agaagggtgc atttttaggt acttggtcaa
    13081 tcatacgagt cgtcttttct gtatctaaga ataacgaaga ggcaccccca acgacaatta
    13141 agcgtgtatc ggttccttca agcatctcaa tcaaatggga caaagaagtt tgatgcattt
    13201 cttcttttcc tttgggagcg ttgaaagcat caacgacaac atcaaaagat gcaagatcag
    13261 tagttgtcaa ctcaaataaa tccttttgta taaacggaac atctgatggc aatttttctg
    13321 gatgacgcac gattgccgtc acgtctaaat ctctggacaa tgcttcgtct aaaataaatg
    13381 aacctgcatg gccagtagct ccaatgagtg caattttcat aaaaaacctc cttttttatt
    13441 cctgtccatt gtaagcagtt gctgaatatt cgtcaaagaa tccgattttt gaaaagctca
    13501 gtgcgcattc ctttcatggt tcgtgatcat tgggtatagt taaattaaga aaactgattg
    13561 ataaaaatag aagttgaagg agataagaaa tgaaagagtg gttagatcgt gcctttttac
    13621 caatgataac atctgttgtg aatcttagac ccttaactgc tttgcgtaat ggaatgactt
    13681 acacgatccc aataattatt gttggctcgc tgtttttctt atttgctcat tttcctgtgg
    13741 attacatcgc tcagtggata gacaaaacag gattttctat gtattttaca caagtatacc
    13801 aggctacatt tgatgtgata gccttttgga tcgtttttgc cattgcatac gcttatgcaa
    13861 aagaagaaga catcgatcgc ttaccagtcg gcataacagc cttgtctgct tatctactgc
    13921 ttattcagcc actaaatact cagatagata ctgtctggac aggtgctgaa gggatactag
    13981 gagcagttac aactgggctc atcatcggat ggggctatgc acgtttagta agacttgcga
    14041 aagggtggaa gcaaagagag aagatacctg aaaatgtttg gcagtctttt acttcattga
    14101 ttccaatcat tgttatagtc acttttacgc ttatcggttc agcggttttt caacagttgt
    14161 tcagaatgac tgttgttcaa ggagtatgga catatgtcca gcttcccatt caatcgttca
    14221 tagacacgtt gtttggtgtg cttttattag gtttttttgt tccgttttta tggttatttg
    14281 gtcttcatgg atctgcgatg gtcaatgggt tggttagtcc cgtcttgcaa gcgaattcat
    14341 tagcgaatgc agaaatctta gcctcaggac aagagttgac ggtggcaaat ggcggacata
    14401 tcgttactca gcaatttctc gatcagttca tgacggttac gggtgctgga ttgacgttag
    14461 gggctgtatt ttttatgacg gtttttgcga aatcgaagaa atacagagaa ttaggaaatc
    14521 ttgctctttt accagccttt ttcaacatca atgaatcaat tattttttcc acaccgatcg
    14581 tgatgaaccc gatgatggct gttcctttta ttttcgcacc ggtcgtttca ggattgatta
    14641 cctattccgc tttatatttt ggattcgttc ctttatttac agcagtccaa gtcccttgga
    14701 caacaccgcc gatcctctcg ggtttcttag taggcggagt gcctgcagcg attttacaag
    14761 gtatcgtgtt gtcaattagt tttttcattt atttcccatt tttgaaaaaa atagattctg
    14821 caaactgcaa aaaggaaaga cagacaaaaa atgatggagc agcagaaaag atgaacgact
    14881 aaaaaacaaa aatactcctg tcgctttaga ttgggaaaat caccaatcta ggatgacagg
    14941 agtatttata gtcatgttta tactagccct ttttggacag ctaattgaac aagaacaact
    15001 aatgtgttca ttcctacgtg tgtgatcatc gatgtccaga tccgaccagt tgctttgtac
    15061 tctaggctga agaagaaacc taggaaaaag tagattagca gatgaccatc attatgagca
    15121 aacgcaaaca gcaaagagct gatcactgct ccgacccaaa aatttgaata tcgactgatg
    15181 atccccagta tactgcggcg gaatacgaac tcttccatga tgggtccagc aacagttgtc
    15241 gccaaaataa aagcgggtgc ttccatgatc atctggatga tattttgtgt attctcggaa
    15301 ggggttgctt tgccaaacaa tacagattct aacatgacag cgatgctttg cagaatcaat
    15361 gcgacaaaga ttccaaccaa tccataaata acaatctttg gagcagaagc tttttttacc
    15421 gctgattcga tcgctaaggg ttcacgttgc ttaaaataaa aaatagctag gacaacggcg
    15481 cctagtatat acatgactgc agttgctgtg atcaattcag acgtagattt tacaagtcct
    15541 atcgaagcta ggatcaaggg tgagataaaa attagaccat agcaaaaaat gctgaggaag
    15601 ctatattttt ttaaagacat gtaattcctc cttttaaggg ataacaagca tgcaaggcaa
    15661 gcttatatcg attgatttca attatatcat aatttcaata agcagctgtc agaataaaat
    15721 ttcttttctt tgatttaaaa gcttgcaaaa aagagggaga atgagtatta ttaaaaatgt
    15781 aggttagcac tcgaaggtta agagtgctaa cagattaaaa aaagagttat cggagggatt
    15841 tatcgtgtta aaaccattag gtgatcgcgt cattatcgaa gtcgcaaaag aagaagaaaa
    15901 aactgtcgga ggaatcgttt tagcttctgc tgctaaagag aaaccccaaa ctgggacagt
    15961 tgttgcagtc ggtgaaggcc gtttgttaga aaacggtgaa aaagttcctg ccgctgtaaa
    16021 agcaggcgac caagttatgt tcgaaaaata cgccggtaca gaagtaaaat acgaaggaaa
    16081 agaatatctg atcgtcgcag gaaaagatat catggcgatt gttgagtaaa tttggaagat
    16141 aaaaaaataa tcaaaaataa tcaatgaggt gaatcaatca tggcaaaaga attgaaattt
    16201 gcagaagacg cacgcgcagc tatgttacgc ggggtagata aattagcaga tacagtgaaa
    16261 gtaacattag gacctaaagg ccgaaatgtc gtactagaaa aatcttacgg ctctccattg
    16321 atcacaaatg atggtgtgac gatcgcaaaa gaaatcgaat tagaagatca ttttgaaaac
    16381 atgggtgcaa aacttgtatc agaagtagca tcaaaaacaa atgatattgc aggagacggt
    16441 acaacgacag caactgttct gacacaagca atcgttcgcg aaggtttaaa aaacgtaact
    16501 gcaggtgcta atcctctagg cattcgtcgc gggatcgaat tggcaacaaa agcagcagtc
    16561 gaagaattgc ataatatctc aactgttgtt gattcaaaag aagccattgc acaagtagca
    16621 gctgtttctt cgggttctga taaagttgga catttgattg ctgatgcaat ggaaaaagtt
    16681 ggaaacgacg gtgtcatcac aatcgaagaa tcaaaaggga tcgaaacaga actagatgtt
    16741 gtagaaggta tgcagtttga tcgtggttac ttatcacaat atatggtaac agataacgac
    16801 aaaatggaag ctgttctaga aaacccatat atcttgatca cagacaaaaa aatctctaac
    16861 atccaagata ttctgccatt gttagaacaa atcttgcaac aatcacgtcc attgttgatt
    16921 attgctgacg atgtagatgg tgaagcattg ccaactcttg tattgaacaa aatccgtgga
    16981 acattcaacg tagtagctgt aaaagctcct ggtttcggcg atcgtcgtaa agcaatgctt
    17041 gaagatatcg ctattttgac aggtggtaca gtaattactg acgatctagg tttagaattg
    17101 aaagatgtaa caatcgaaaa cttaggaaat gcttctaaag ttgttgtaga taaagacaat
    17161 acaacgatcg tagaaggttc tggagaaaaa gaagcaatcg aagctcgtgt gcaattgatc
    17221 aaaaaccaaa ttgctgaaac aacttctgat tttgaccgcg aaaaattaca agaacgctta
    17281 gctaaattag caggtggtgt agctgttgtt aaagttggtg cagctactga aacagaattg
    17341 aaagaattaa aattacgcat tgaagatgca ttgaatgcta cacgtgccgc agtagaagaa
    17401 ggaatggtct caggtggtgg tacagctcta gtgaacgtta tcagcaaagt ttctgctgta
    17461 gaagcagaag gagacgtggc aacaggtatc aagatcgttg ttcgtgcttt agaagaacca
    17521 attcgtcaaa tcgctgaaaa cgctggttat gaaggatcag tgattgttga taaattgaag
    17581 aacgtggaac taggcactgg tttcaatgct gctactggag aatgggtaaa catggttgaa
    17641 gcaggtatcg tagacccaac taaagtaaca cgttctgcct tgcaaaatgc cgcttccgtt
    17701 tctgccttgt tattaacgac agaagcagtc gttgcagata aaccagaacc agcagcgcca
    17761 gcagctcctg caatggatcc atcaatgatg ggcggtatga tgtaagaaaa agcaacttgc
    17821 ttatctacaa ctgatcatta gtgaaagaca gataaaaaga ggctggaaca gaagcgttta
    17881 actccaagaa ataagaagaa attcacggaa attgcttttc aaatttttgt gaatttcagc
    17941 ttatttccga aggagttgct tctgcttccg ctgtttatac gtgtttagag cgtgagacaa
    18001 aagtgttctt tacttttgtc ccacgctctt ttttgtgtgt aattataatg aaaatagtta
    18061 tttttaatag ttataatatt ttatctatta aaagtaaata tccattc
//
LOCUS       DAMYXM010000051        17433 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.052, whole genome shotgun sequence.
ACCESSION   DAMYXM010000051 DAMYXM010000000
VERSION     DAMYXM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.052"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(111..1079)
                     /locus_tag="PNS21_001974"
     CDS             complement(111..1079)
                     /locus_tag="PNS21_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDI5159678.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELKKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            1311..2144
                     /locus_tag="PNS21_001975"
     CDS             1311..2144
                     /locus_tag="PNS21_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDI5159679.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            2178..3017
                     /locus_tag="PNS21_001976"
     CDS             2178..3017
                     /locus_tag="PNS21_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDI5159680.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            3043..3603
                     /locus_tag="PNS21_001977"
     CDS             3043..3603
                     /locus_tag="PNS21_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDI5159681.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            3802..5523
                     /locus_tag="PNS21_001978"
     CDS             3802..5523
                     /locus_tag="PNS21_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDI5159682.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            5688..6830
                     /locus_tag="PNS21_001979"
     CDS             5688..6830
                     /locus_tag="PNS21_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDI5159683.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            6846..8057
                     /gene="thiI"
                     /locus_tag="PNS21_001980"
     CDS             6846..8057
                     /gene="thiI"
                     /locus_tag="PNS21_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDI5159684.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            8630..9277
                     /locus_tag="PNS21_001981"
     CDS             8630..9277
                     /locus_tag="PNS21_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDI5159685.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    9406..9622
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9670..12315
                     /locus_tag="PNS21_001982"
     CDS             9670..12315
                     /locus_tag="PNS21_001982"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDI5159686.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            12625..13938
                     /locus_tag="PNS21_001983"
     CDS             12625..13938
                     /locus_tag="PNS21_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDI5159687.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            13928..14581
                     /locus_tag="PNS21_001984"
     CDS             13928..14581
                     /locus_tag="PNS21_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDI5159688.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            14636..15307
                     /gene="radC"
                     /locus_tag="PNS21_001985"
     CDS             14636..15307
                     /gene="radC"
                     /locus_tag="PNS21_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDI5159689.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(15202..16554)
                     /locus_tag="PNS21_001986"
     CDS             complement(15202..16554)
                     /locus_tag="PNS21_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI5159690.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
     gene            complement(16620..>17433)
                     /locus_tag="PNS21_001987"
     CDS             complement(16620..>17433)
                     /locus_tag="PNS21_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HDI5159691.1"
                     /translation="ITGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLE
                     LKLQELELNYSYIVKVNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEI
                     TKLMAYMQKASADVEKELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNI
                     GSFGEDVYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRK
                     NEVNKVNQRKEFFKVSLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
BASE COUNT         5745 a         2986 c         3497 g         5205 t
ORIGIN      
        1 aaatttacga aaattgtttc tcaaattttt gtaaattttc gacttagttt tcgaagaagc
       61 tatttttgtc acagcctctc tttttaagtt tagctattag ataatgatgt ttagttttgg
      121 ataaacgaaa tcaattcttc acccgacaaa ccatagttta cagcaatcaa tagttttaaa
      181 cgtgcttttt gactattaat actgttacag aaaataatcc ccattttttt tagttctttt
      241 cccccgcctt cataatcata aacaggctct gcgattccat tgaaacaacg agaaacaagt
      301 actacaggga ttttttggac aagaagcttt tccaaagcag ctaaggcttg tggagggaga
      361 ttccccgcac ctaaagcttc gattaccaaa ccgtctactt tcgtatgggc gatggcttcc
      421 aataagtccc cttgcatccc agcatatgct ttaacgatcg ggatagtccc atctacagcg
      481 gaaatgtcca gtcgctttgt ttctaaaagt tcctgcaaga ataaaattcg attcttagtt
      541 actaagccga tgggacccaa tgtaggtgtt cggaaagtag caacatttgt ggtatgggtc
      601 tttgtcacgt agcgtgcaga atggatctca tcgttcatga ccaccaaaac ccccttatct
      661 agcgcttctt cacaacttgc gacacgaatc gcactttcaa aattataaag gccgtcactt
      721 ccaagttcat tacttgaacg cattgcccca gtcaaaacaa ttggtaattt gttgccaatc
      781 gttgtatcaa gaaaaaaagc agtttcttct aatgtatctg taccgtgtgt gatcaccact
      841 ccggatatac ctgatgcata agcggacaag atacgttctt ttaataccaa catgtgagaa
      901 ggcatgatgt gtggtgaggg aagattgaag atatcttcaa ctaataattc tacatgcttg
      961 gggattgtga tttctgcatc taataatggg tttgccacat caggactaac accgcccgtg
     1021 agcatgtttt ctttcatggc gatcgttccg ccggtgtgta gtacaagtat ttttttcatt
     1081 ttacagactt ccttacgaat aattttgcat agccgttaaa tcttattgta tactatataa
     1141 aagagttttg ctatcagtga agaataacga atagcctaac ttatcataaa aaagctgacc
     1201 ttattaaaat ggaaaagact caaccttgac ctcctaatac aatggagtat ctggttgagt
     1261 ttcttttaca agaaagcagc caaggaaatc aatcacgaag gtggccggaa atgatcagaa
     1321 tcggattaac atcgtttaaa gaacatgaca aattaactgg taaaaagaat aacactcttt
     1381 atgaatatgc agggtttcta cccttagtgg aattagatac tgcctattat ggaattcccc
     1441 gaaagtcctc agttgagaat tggataagtg aagtacctga atcatttcgc tttgtagtca
     1501 aagtctatca aggaatcagc ggtcaaggag actggaagcc ttattatcag accgaagaag
     1561 agatgattgc gcatttttta gatgttatgt cgccaatggt agatgctgga aagatgtttt
     1621 gctttttagt ccaattcccc tcatcattca aatgtacgaa agaaaatgtc cagtacttgc
     1681 gcaaacttag aaagtggttt gagggttatc ctgtttctat agaattgcgg gattattctt
     1741 ggtacagcga tgaatttctt gaacgtacaa gaaactttat gtctagagcc gctttttctt
     1801 tagccatcat cgatgagcca cagttgctga atacaacgat tccttttgat ccatttgtca
     1861 ccaatccgaa ctttgctctt tttcgattcc atggtaggaa tgcgcaagga tggcaagcaa
     1921 atgataaaga gtggcgtaaa aaaagaacgt tatatcgtta ttcagaaaaa gaactgcaag
     1981 aactagcaca tgaagtcaaa gtagttgatt ctaagacaaa agaggtaggt gtgattttta
     2041 ataataattc tggtggtgat gcggctgaaa atgcattagc actgcaaaaa atattaaata
     2101 tcaagccaga tggactcaat ccaacccaaa tggatctgtt ttaagggctt attttattca
     2161 agaaaggggc gaagtctatg atcaaagcta ttttttttga tattgacgga acattggtca
     2221 ccaatcgttc aaaagcgttg gaatcaacaa aacaagcaat tgaatatgct cggaaaaacg
     2281 gcattttatg cggtgtagca acggggcgca gcccagtaaa gatcaaagaa atcattgatg
     2341 aattggaatt agatatgtat gtcgtgtata atggtcaact ggtttttact gcagatcgca
     2401 cgattatcga ccaccctttt gaacaaaaag tattggaaca tattgttgaa tttgcagatg
     2461 agaaccatcg acaaatcgtt tttggtgcga gaaatcggtt agatggatca acgacgatgc
     2521 tattaggaca atcgattttt atcaagcgac tagtcagctt tttaccgcgt aaattcccgg
     2581 tacgtctgat gaaaaaaatc ctccaggttt ttagccctca ccgacaaaag gatcgttatg
     2641 aaacgttaga gatattaaaa gaaccaattt atcaatgtat attgctcagt ccggagtcag
     2701 aacagaaaaa gttagaacat cgctttcccg agtgtacgtt tcagcgttca aatacgtata
     2761 ctgtagatat catcccaaaa ggtggttcaa aattactagg catccaagct ttcgcaaatg
     2821 ctgtcgaaat tgaaatggaa gaaattatgg cattcggcga tcattacaat gatatcgaga
     2881 tgctgaaagg tgtaggaatt ggagtggcca tggggaatgc tcagatcgaa gtaaaacatg
     2941 ctgcagactt tgtgactcaa tcaaatgaag aagatgggat atacgaagga ctgaaacact
     3001 tcggtctgat ttattagtca atgaaagaag gaaaaaatca gtatggaagc agagacacct
     3061 tttcaaaaag tagaaacatt ccacaaaact tttgatccaa gaagaccaga gatacccacg
     3121 gcttttacag aaaatgaagc tcatacacgt gctgcgttca aaatagaaga acttgttgaa
     3181 tttgttgcag ctacttcacc taataaagaa caactgcatg aagctgttat gctgctgcac
     3241 gaagcattag ataaagcaga agaaaaaata cttggtaaaa cacagtgggg aagtgatccg
     3301 ctggttgaac aagtagatgc actgacagat atcctttact ttacttatgg ttctttttca
     3361 ttactaggaa tagtgcctga tcgaatattc gaaatcgtcc atcaggcaaa tatggggaaa
     3421 ttatttccag atggaaaacc tcattatgac cctgtaacaa ataaagtgtt gaaacctgaa
     3481 aactggcaga tggaatatgc tccagaacct aaaatcgcgg cagaaatcca acgtcagatt
     3541 caagaagcga ttgctaagaa gaaatccgct gatataggca tgtgcacaaa tgaaaaagag
     3601 tgatcagagt ttcactattt ttgttatctt tatacgatgg aaaaacaaaa gattttcgtt
     3661 taagttagtg aatgcatgaa aatagtatgc tataatctag aatagatgtc ttttgcacga
     3721 gagacgtatt atttggattt atgccaagca gaacgccatc tgttgcataa aagatgcata
     3781 ctgaaaacgg aggaaccata aatgggaaat aatgttatca tcggaatcgt cattgcaatc
     3841 attatagtcg ccattgtcgt ctttttggtt agttacttga tgaagaaaaa aaaccaagat
     3901 cgattggatg ccttagaaaa acgcaaggaa gaattatttg acttgccagt gattgaagaa
     3961 gtcgatgatg ttaaaaaaat gcatctggtt gggcaaagcc aaaatacctt tcgtgaatgg
     4021 agtcaaaaat ggaatgacat atctacttcc tcgttcgctg aactagaaag tcaaattttc
     4081 gaagtggaaa acctcaatga aagttttcgg ttcttaaaag gaaaaaaagc agttgttgaa
     4141 gcagaagcta caatggacca aatggagaaa caagtgacgg aaatccgtga aggtctaaag
     4201 gaattgcggg aaacggaaga acgcaactca ctagctgttc agcaggcact tgacgtctat
     4261 gaagaactga aagaaaaagt cagagaagac gagcatgaat acggaccagc tctttttgaa
     4321 ttgcaaaaac agatcaaagt gatcgaaaaa gaatttacgc agtttgtgac tttaaacact
     4381 tctggagacc ctgttgaagc ccgcgaggtt ttggataaag cagaaaaaca tacgtatgaa
     4441 gtggaagctc tgatgaaaaa aattccaccg ctgtatgagg agctccatac cactttccct
     4501 gaacaacttg aagaaatcag tgatacttac gagaaattga tggaagaaca atatgtattc
     4561 ccagaagaag atcttgctga agatatcgca aaagtaagtc gaagaattga aaattcatta
     4621 gccaatcttg aaaaaacaga agttgaaacg gtcgaatttg aaaaccgtga aacagctgat
     4681 ttgattgatt cgttatatga tatcttagaa cgggaaatgg aagctcagag atatgtaaaa
     4741 actaaccaat caacgattgc agagtatatc aaacatacaa caaagaataa ccgtcaatta
     4801 ttgattgagc tggatcacac atctcaaagc tatacattga accacaatga aattggccgt
     4861 gtacgaggtt tccaaaccga agtggaagaa atggaacgtc aaaatgaaca gatgattcca
     4921 cagatccgtc aacacgaaat tccttattca gaagtgcgca cgttctataa aacagtcttc
     4981 aaagtcttag aagatatcga aaagcaacaa gtagagatcg acgactcatt acatgaattg
     5041 agaaaaggcg aaaaagaagc gcaagaaaag atcgatacat tcgaattcaa attgcgcagt
     5101 ttgaaacgtt ttgtagaaaa acaacgtttg cctggcttgc cgaatgatta cctagaattc
     5161 ttttttgttg ctactgaccg tattgaagaa ctaagtgttg tcttgaataa aattcgagta
     5221 aacatggaag aggtcaatcg tttagttgct ctatgtgaag aagatctaga attattagat
     5281 aagaagacac atgatttagt agatgcagca gcattaacgg agcaaatgct ccaatatgca
     5341 aatcgttatc gtcatacaca cgaagaagtg agagaagcaa ttgataaaag ctactattta
     5401 ttcaataaag aatatcacta tcaagaagca ttagacgaaa tcggtacagc attagagcga
     5461 gtagaacctg gtgctttcaa acgcatcgaa gatttttatt ttaatcatcc agacttagta
     5521 taaagcaaaa aagttagtaa accagcaaga gcaagacgga ttattgtctt gttcttgtct
     5581 ttttttgtat atttgaaaat tgaaacaagt gaaaatttca cctgctcttg aaagcacctt
     5641 ggacaaccgt tataatagaa aagaatttca aaaaagaggg gtttcttatg atatattttg
     5701 ataacagtgc aacaacccca atctttccgc aaagcttaga tgcatatgta aaaaccagtc
     5761 agcggattat tgggaatcca tcaagcttgc atgatttagg caatcaagct aaccggttgt
     5821 tgcagcaagc aagaaagcag atcgctgatt tgatccatgt agaaccagaa gaaatctatt
     5881 ttacttcagg cggaacagaa ggagataact gggtattaaa aggaacgatg atcgaaaaaa
     5941 gagaatatgg gaatcatatg atcatttcag gagtcgaaca tccggccgtc agcgagacag
     6001 cagaacaatt aaaagaatta ggaatcgaag taagtatcgc cccagtggat aaacgcggat
     6061 ttgtacgtgt cgacgaattg aaagaattaa taagaaaaga gacagtatta gtctctgtta
     6121 tggctgtgaa taatgaagta ggatcgattc agcccatcca ggaaatcagt gatcttttgg
     6181 atgattttcc taaaattcat ttccatgtag atgctgttca ggcgattgga aaagtaccat
     6241 tctcgcaatg gttgacagaa agagttgatt ttgcaacatt ttcagctcat aaattccatg
     6301 gcccacgggg aactggtttc atttattgga aaaagggtcg tcgtttagca ccattactga
     6361 caggtggagg gcaagaaaga aatcagcgaa gtgggacaga aaatgttcca ggtattgtgg
     6421 ctatggttaa agctttacgt ttatcattag agataaagga acaaaaacct gagcagacac
     6481 aacgcttaaa agtagctcta atcaatgcgt tgaagacgta tgagaaagtg acgatttttt
     6541 cagaaggaac tgactatgca ccgcatattt tatgttttgc tctaaaagga attcgcggcg
     6601 aagttctcgt ccatgcctta gaagaaaaac aaatatttgt ttctactact agtgcctgct
     6661 cctcacgtaa acatatggca agcagtacat tgcacgcaat gcatgtacca aatgatctgg
     6721 ctacatctgc tgttcgaatc agcttagatg aaaacaattc gcttgcagaa gtcgagcaat
     6781 ttatgattat tttcaagcag ctttataaga aattttctgc aatcaattaa aagaggagtg
     6841 ttcttgtgaa atacagtgaa attatggtcc gttatgggga actttcgaca aaaggaaaga
     6901 atcgaaaaag ctttatcatg caactagcac aaaatgtcag acaagctttg aaagactttc
     6961 cagaaatcaa aatccatgct gatcgggacc ggatgcatct tttattgaat ggcgcagacg
     7021 ggcagctagt tattccaaaa ctagcaaaaa tatttgggat ccaaaatttc tctccaagta
     7081 ttcgtgtaga aaaagatgtc gaagttttga aacaagctgt tcaagacatc atgaaagaaa
     7141 tttatactgg gaaagaaaca tttaaaatta tcgctaaaag aagcgatcac caatttgaat
     7201 tgacaagtaa tgaattgaat caaacattag gtaatgctgt ttttgatatt ttcccgcata
     7261 tccaagttca aatgcgtcaa ccagatattc cattacgtgt agagattcgg cgtgatggcg
     7321 cttatctatc gtatgaaacg atcaagggag ctggaggatt gcccgtagga acaagcggaa
     7381 aaggaatgct tatgctttct ggggggattg attctcctgt agcagggtac tttgccatga
     7441 agcggggagt ggaaattgaa gctgttcatt ttgctagtcc gccgtataca agtgaacaag
     7501 cactgcaaaa agcaaaagat ctaacagcca aactagcacc ttatgtggga acgatccatt
     7561 ttatcgaagt gccatttacc gagattcaag aagaaatcaa aaagtcagtg ccacaaggat
     7621 attggatgac aattactcgt cgaatgatgt tgcgtttgac cgatgaaatt cgccgtattc
     7681 gtcatggttt agtcattttg aatggtgaat cattaggtca agtggcttca caaactttgc
     7741 aaagtatggt ggctatcaat gaagtaacga atacgccaat cattcggcct gtagcgacaa
     7801 tggataaact tgaaatcatt gaagtagcag aacagatcga tacatttaat ctttctatcc
     7861 agccttttga agattgctgt acgatttttg ctcctccaca gccaaaaaca cgccctcaac
     7921 tggaaaaggt ccatcaatat gaagagcgtt tagcaataga tgaaatgatc caacgagcac
     7981 tagctggctt gaagatggaa gagatacaag cagaatcgtc taatgaaaaa actgaagaat
     8041 tttcagaatt gctataagag gttatgaaaa tagtgttttg accagagtat tagagaaaca
     8101 aaacgaaaaa atagctccat gtattttacc gtttttgaac taatgtcgaa gattagctga
     8161 ttgaatacca tttatcaaga agttatggaa aagccggttc ttttttacta actgaaaaat
     8221 aaaattttat ttagagatca agctacagtt gataaatgat ataaaaaaga gaatttccca
     8281 tgaaatagcg aggttgtgac agaagtgggc agcttcaaga aataagaagg aatttacgaa
     8341 aattgctttt caaatttttg tgaaatttcg gcttattttt cgaagaagtt acttctggaa
     8401 caccgtgtat cagaggttgc gaaaaagctg ccctgcatca agtaataaga acagccaaac
     8461 aaaaatagtt cctcatattt tacgtttggt tgagcttatt accgcagatg caagcttttg
     8521 aacaccgttt tatttgtctc acaccctttt ccttcctact tgtaaccttg ctcccgtgtt
     8581 agaaacatgt tacactaaat atgtgaaatt attaacaagg agcgtcgtta tgcgagaaaa
     8641 aacagataca ctgccaaaag caactgcgaa aagattacct ttatatttac gctacttgaa
     8701 gatgctggat gactcaggta taagtagaat caaatcaaat gagttcagcg aaatcaccca
     8761 aattccttct gcaacgatcc gcagagactt ctcgcaatta ggcgagctag gcagaagcgg
     8821 atatggctat gatgtccctt ttcttatcga tgtattcaat aatatcctta atactaaaga
     8881 agaaaaacga atcgcattag taggatatgg aaatcttggc aaagcattga aacacaacaa
     8941 ttttagaaga aatgaaaatc taaatatcgt gtgcgtcttt gataatgatc cagcactgat
     9001 caatcgagta attgatggag aaatgatcta tccgattgat cgtttcgcgg agatagccaa
     9061 agcaaaaaat gtgacagtag caatctctac ggtaccaagt aaatattcac aatctgctat
     9121 tgatgaaatt gtcaaaggaa atgtaaccgc tatacttaat tttgcgcctg acagagtaac
     9181 tgtcccagct tatgtcaatg ttcaatacat tgatttgaca accgagttgc agacgcttat
     9241 ttactttgat gagaattact cagaagtttt ttcttaggaa aatttgagta ggcttattga
     9301 tcttgcgaga actcgtcgta attctttcaa aaatttttta gtactaaatg aagtggtttt
     9361 ttcacgacca cttgttgact aaaaaaaaaa accattgtag aatgaagcat aacgctgaac
     9421 aagaggagta actggcacat tcattcttag agagagaact atctgctgaa aggttctcaa
     9481 tgacaaccag tgaaggtagc ttgggagttt ctgtcttgaa tggagtagag atagacgagt
     9541 cgtgatcgtt aactcacttg agaggcaata atgattgatt attgcaattt aggtggtacc
     9601 gcgtaaatca tacgtcctag aatacgaaag tattttagga cgttttttag tttaaaggag
     9661 gaaaacagca tgacggaaga aaataaccta tcaacaaaat ataatccgca agaagttgaa
     9721 gctggacgtt atcagcattg gttagaacaa gatttgttta aaccgagcgg agataaaaaa
     9781 gcaaaacctt attcaatcgt aatcccgccc ccaaatgtta ctggaaaact tcatttaggg
     9841 catgcttggg atacgacctt gcaagatatg ctgattcgtc aaaagagaat gcaaggatat
     9901 gatactttat ggctgcctgg tatggaccat gcaggaatcg cgacacaagc aaaagtagaa
     9961 gcgaagctta gagagcaagg gatttctcgt tatgatctag gtcgtgaaaa gttcatcgat
    10021 caagtgtggg aatggaaaga agagtatgcg agccacatcc gcgaacagtg ggctaaaatg
    10081 ggcttatctt tagattacag tagggaacgt tttacattag atgatggatt atctgaagca
    10141 gtacgtaaag tttttgttac cttatacgaa aaaggtttga tttatcgtgg tgaatatatc
    10201 atcaactggg atcctcaagc acgaacagct ctatctgata tcgaagttat tcataaagat
    10261 atcgaaggcg ccttttatca tatgagctat gaactagcag atggatcagg cgtagtagaa
    10321 attgcgacaa cccgtccaga aacgatgttg ggagatacgg cgattgctgt tcatccagaa
    10381 gacgagcgtt atcaagcgct catcggcaaa aaagtgattc tacctttagt caataaacaa
    10441 atcccaatca ttgcagatga atatgtagat atggaattcg ggacaggtgt agtaaaaatc
    10501 actccagcac atgatccaaa cgactttgag gtaggtaatc gtcatgatct tccacgcgta
    10561 aatgttatgg atgatgatgg tacgatgaat gaactagcag gaaaatatgc tggaatggaa
    10621 cgttttgctg ctcgtaaagc aatcgtgcaa gatctgaaag aaatcggtcg attgattaaa
    10681 atcgatacga tgatccacag tgtgggtcat tccgagcgga caggtgtcgt tgttgaacct
    10741 cggctgtcta ctcaatggtt tgtaaaaatg gcgccgttag cgaaaaaagc cattgataac
    10801 caagaaacag atcaagcagt ggaattcttc cctccaagat tcaatcaaac gtttttacgt
    10861 tggatggaaa atgtccatga ttgggtaatc tcacgacaat tatggtgggg acatcaaatt
    10921 cctgcatggt atcacaaaga aacaggtgaa atgtatgtag ggatggaagc accagcggat
    10981 agtgaaaact gggtacaaga tcctgatgtt ttagacactt ggtttagttc agctttgtgg
    11041 cctttctcaa caatgggctg gccagatacg gaaagcgaag attataagcg atacttccct
    11101 actagcactt tagtaaccgg ttatgacatc attttcttct gggtaagcag aatgattttc
    11161 caaagcttgg aatttactgg cgaacgtcca tttgagaatg tcttgattca tggattgatt
    11221 cgtgacgaac aagggcggaa gatgagtaaa tcattaggta atggaatcga tccgatggaa
    11281 gtaatcgaaa agtatggagc agatgctctc cgctggttct tatcgaatgg ttctgcacca
    11341 ggacaagatg tccgcttcag ctatgaaaaa atggatgctt cttggaactt tatcaacaaa
    11401 atctggaatg cttcacgttt cgttatcatg aatatcgaag gaatgaccgt agaagagatc
    11461 gatttatctg gagaaaaatc agtggctgac cggtggatcc ttactcgttt gaatgaaacg
    11521 atcgaacgag taactgagct atttgatcgt tttgaatttg gtgaagcagg tcgacaactg
    11581 tataacttta tctgggatga tttttgtgac tggtacatcg aaatgagtaa agaagtactt
    11641 tatggagatg atgctgtttc aaaacaaacc acccgtagca tcttagtcca tacacttgat
    11701 cagatcctac gtttattgca tccaatcatg ccattcgtta cagaagaaat atgggaacat
    11761 attcctcatc agggaaattc tctagtagtg gcagaatatc ctgttgtacg tccagagttt
    11821 gatgatgaaa cagcttctaa agggatggaa gtattgaaag aattgattcg agccgttcga
    11881 aatattcgtt cagaagtgaa tacaccattg tctaaaccaa ttacgttgct aattaaaaca
    11941 aacgatgcta aaatcgatca atttttagtt gaaaatacaa gctatatcga acgcttctgt
    12001 aatcccgaag agttgacgat atcaagtgag atcactgcgc cagatctagc gatgtcagct
    12061 gttctgacag gtgcagaaat cttcttgccg ttagcaggat tgatcaatat tgaagaagaa
    12121 atcaaacgat tagaaaaaga actggaaaag tggacaagcg aagtaaaacg tgtccaagga
    12181 aaactttcta atgaacgttt tgtttctaac gcgccggaag aagtagtaga ggcagagcgt
    12241 gcaaaagaaa aagattattt agagaagcaa acggctgtca aagaacgaat cgaaagtttg
    12301 cgtactattc aataagaata gaaaaagaga gtgtgaaatc agctagctat tactaaaagc
    12361 tagctgattg aacatcgtta caggaaagag tttgagcctc actcttagct tcgattcata
    12421 aggagatgtg acataaattt tgtcgcatct cttttttagt ctactcttat tatgtaggag
    12481 aaggagggag gtggatcatg tatcaagagt caataaagta gttgataaaa tcgttcctct
    12541 ctctgtttct tttctctttg tcatactttt atttttctta tgtgttctat ttgttataat
    12601 aaaagagaaa tgggggaatt gttgatgacg attgaagaag caatagaatg gatccatagt
    12661 cgattaccat ttggttcacg tcctggcctt gaccgagtcg aagctttatt agaagtggta
    12721 gggcatccag agagaaaagt aaaaacgatc catatcgctg gaacaaacgg aaaaggttct
    12781 actgtagctt atttgagaag cctattagaa gaggctgggc tgacagtagg tacatttact
    12841 tcaccttata tcgaaacgtt caatgaacgg atcgcaatca atggcaaaag cattccagac
    12901 aatgatttga tccagcttgt aaaaaaatat cagccactga tcaaacaact tgatcaattt
    12961 gaagcagtag ctggtattac tgaattcgag acattaacag caatggcatt tgattatttt
    13021 ttgatgaaga aagtggacgt agcgattatc gaagttggct taggcggctt attggattct
    13081 accaatgtag ctgtaccaat gctgacggga ataacaacga ttggacttga tcataccgaa
    13141 atacttggag atacaattga agaaatcgct gcacaaaaag ctggaatcat caaacaaggc
    13201 gttccagtag taacaggaaa tattgcccca gaagctttta ctgtcatcca acaaacagct
    13261 aaagcaaaaa atgcaccgct ttatgtatac ggggaaaatt ataaaataaa ctatctgcat
    13321 cctgatgaga tgtggggaga agtatttgaa ttttttaatg aaataggaaa gatccctcaa
    13381 ttaaccactc cgcttttagg gcaacatcaa gctgaaaatg caggagtagc catcgaattg
    13441 tattttgttt actgtcaatt ggaaaagctg cctttccgag aaaaagatgt tttgaaggga
    13501 ttgaagaaat ctcgatggcc aggaagaatg gagcgtttga gtaacgagcc attagttgtt
    13561 ttagatggtg cccataatac gcatgcaatg aaccggcttg ttgagaattt gaaaaaagaa
    13621 ttcaaaggat atcaaatcaa cattttgttt tccgccttac gaactaaaaa tgttagagaa
    13681 atgctagaac gattgcagca agtaccaaat gtccatattt atttgacgac ctttgaacat
    13741 ccaaaagcca tcgaattaag tgaagtatca gatttagcaa gtgagagagt atcaatcgtt
    13801 tcattgtggc aattcggatt agcagatata ttggaaaaga tgacaaatga agacgtatta
    13861 gttgtgaccg gttcacttta tttcatttca gaagttcgcc atttattaat acaattagga
    13921 ggagatcatg aagtataatg cagtgatttt tgacatggat ggtttgctgt tcgacacaga
    13981 gttagtgtat tacgaagcca cacaagtagt agcagacaag atgggttttc cttatgataa
    14041 agacctgtat ttaaaattct taggtgtttc agatgaagaa gtatgggcga attatcatat
    14101 gattttctcg gaatttggca aaacaagtgt acaaagattc atagatgatt cataccagga
    14161 aacgcttgaa cgtttttcgc aaggagcggt acaaatgaag cccggtgtgt tggaactcct
    14221 ctctttttta gaagaacatc agattcccaa agttgtcgct tccagtaacc aacgagcagt
    14281 cattgaactt ttattgaaga agaacaatct gtttgaacga tttgagacga ttgtttctgc
    14341 agaaaatgtt caacgagcaa aaccagaccc agaaattttc ttgttagcta atcagaagct
    14401 aggtacagcg aagaaagaaa cattgatttt agaagattca caaaatggta ttttagcagc
    14461 ttctgcggct gagatcccgg tagtcatggt accagatctg cttccaccat cccaagaatt
    14521 gatcaaaaaa acagctgggg ttgtttcttc tttacatgaa atccctgcat tgttaaaata
    14581 aaaatccatc tcttttcgta tcttatttta gataagatag aaaggagatg tttttttggg
    14641 aaaacgattg atcaaagaag tacctactag ctctttacca agggaacgta tggaaattta
    14701 tggcgcggaa gctttatcgg atcaggagct tttggcaatc ttactgcgta ccggtcaaca
    14761 cccctatagt gtgatgtcga ttgctggaaa tctgttaaaa acgtttggag gactggcctc
    14821 tttacgacag gctactttgc atgagttaga agagattcga gggataggac gcgtgaaagc
    14881 aatcgaaatc aaagccttga tcgaattagg tcgacgaatt cagacggatc atgaaagagc
    14941 ggctccaccg gttcgtacca gctacgaatt ggcccagcaa ttgattctgg aaatgaaaga
    15001 tcacaaacaa gaacatttgg tttgtattta tttggacacg aagaatcaag tgatcttaaa
    15061 aaaaacagtg ttcatcggtt cgctgaatca gtcagtcgct catcctagag aaattttcca
    15121 ttatgctgta cgttattgtg cagcaaggat cgttttagcg cataatcatc cttccggaat
    15181 atataagtta gtacacataa attatataag gaaatttact gttatattgc catctggaaa
    15241 tactattatg ttatcgataa ttgaacgcca taatgcattt ttttcttctt ccgaagattc
    15301 tttataaatt tcatcgaatt cttttgatag aataaattcg acatttttta tatctatttt
    15361 agctggctct acttcaattt catttaattc tttcattaaa gcttctcgat cttttttgta
    15421 ttcctctaag tctataactt cattcaagta ggctttcttc aatctatcta atttattctc
    15481 aatatttttc ttactatttt tctttttaat aatctttggt tgtttttttg aatagtttat
    15541 ttgtatttgt tcaaattttt gtttagtagc ttcaaaaata taacgttcta aatttttttc
    15601 gtaaaaagcg cgcttatttg tgcaacgtaa tttattattt cttcgtttta cacatctata
    15661 acaagtcctt tcgtatatag attctgtacc gtcttttttc ttatatcttg ctttaatagt
    15721 ttgaccagct actgatccct gacagtcaga acattttaat aaacctttaa aaatgtaatc
    15781 acgttttgca tttgttttta aatttttagg taaggctttt tgaacagcat aaaaaagttt
    15841 tttatcaata ataggaggac agtaattttt attatttctt aattcaccaa tgtatttttt
    15901 gttcgtcaac atatttttaa cactttgata atctctaacg attccatact gagagcctaa
    15961 ataataaaca gtttttctta aactaccact agaaagaaag tagttgaata tatctatcac
    16021 gataggagca tcatcattta ttactaattt cttattttct atagagtagc ctaacggagt
    16081 tttcccgctt actacttcac cttgtgcaat tttatttgcc ataacagctt tgatgcgttc
    16141 tgaatcaatt tgggcttcca attcagcaaa actcattact tgtgcaatga aagttctgcc
    16201 atacgctgta gttgtatcat aatattgttg agaaacagca ttccaagaaa cattgtgttt
    16261 ttctaaaatt tcttgagtgt ttaaataatg tcgtagattt ctaaaccatc tatctaattt
    16321 tgtaaataaa attagatcta ttttattttc ttttacatct tgtaatagtt tttgaaattc
    16381 atcacgattt attttttgac cagaaatacc accgtctaca tattcattaa ctactttcaa
    16441 atccttgtgt tgttcgatat attcatagag ggtttctttt tgttctctaa gactatctcc
    16501 atgtttggct tgttgttcag ttgatactct catatataat gctactcgtt tcatgttatc
    16561 tcctttcgaa tgtatgttct tatcgttgta aaagaaaagc ccgaaggcta atctttttac
    16621 tacacatctg ttaattctaa tgatcttctg tattgttcag cttgagctag tttagtaaaa
    16681 gatacagttc cgttataatt ttctaaaaca actttttcaa tttcatcaag agatacttta
    16741 aagaattctt ttcgttgatt aactttattg acttcgtttt ttctaaaatg cgaatgtagt
    16801 tgattttcta atgttggtgc atcttctgaa aatatcattg catgaacatc aaattcaaaa
    16861 ggtacagaag cgctactaag ttccttaatt ctatccatag gttctaaccg tctagtcatt
    16921 cctattttat agacatcttc accgaaagat ccgatattac tgatgacata aacatagcca
    16981 gctctagtat taagttctct ttgcaataca tcttctttga cagtttctag ttctcttaat
    17041 ttctcttcca gttctttaat tttatcagca tagagttctt tttcaacatc agcactagct
    17101 ttttgcatgt atgccattaa cttagttatc tctgagttaa actgtcgttc ttctttatca
    17161 atgcgtttct tttcacgttc tatttcacgt cttacctttt cttcttcaat catttgttct
    17221 tttattgctt tttgctgttc tttttcatcg ttaactttaa ctatgtatga atagttgagt
    17281 tctaattctt gaagttttaa ttcaagtaat tcttttttga attctgcttc atcgttttta
    17341 aaaagcttgt ttattttatc aaaagttctt ataatttttt ctctagttga atctacgtta
    17401 gaaatagtaa gtttattgag aagtccagtt att
//
LOCUS       DAMYXM010000052        17006 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.053, whole genome shotgun sequence.
ACCESSION   DAMYXM010000052 DAMYXM010000000
VERSION     DAMYXM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.053"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..125)
                     /locus_tag="PNS21_001988"
     CDS             complement(<1..125)
                     /locus_tag="PNS21_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HDI5159692.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(229..540)
                     /locus_tag="PNS21_001989"
     CDS             complement(229..540)
                     /locus_tag="PNS21_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDI5159693.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(542..739)
                     /locus_tag="PNS21_001990"
     CDS             complement(542..739)
                     /locus_tag="PNS21_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HDI5159694.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1133..2860)
                     /locus_tag="PNS21_001991"
     CDS             complement(1133..2860)
                     /locus_tag="PNS21_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HDI5159695.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2853..4046)
                     /locus_tag="PNS21_001992"
     CDS             complement(2853..4046)
                     /locus_tag="PNS21_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HDI5159696.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4233..4877)
                     /locus_tag="PNS21_001993"
     CDS             complement(4233..4877)
                     /locus_tag="PNS21_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI5159697.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4971..5105)
                     /locus_tag="PNS21_001994"
     CDS             complement(4971..5105)
                     /locus_tag="PNS21_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDI5159698.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5107..7239)
                     /gene="feoB"
                     /locus_tag="PNS21_001995"
     CDS             complement(5107..7239)
                     /gene="feoB"
                     /locus_tag="PNS21_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDI5159699.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7236..7709)
                     /locus_tag="PNS21_001996"
     CDS             complement(7236..7709)
                     /locus_tag="PNS21_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDI5159700.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8110..8334
                     /gene="nrdH"
                     /locus_tag="PNS21_001997"
     CDS             8110..8334
                     /gene="nrdH"
                     /locus_tag="PNS21_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDI5159701.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8493..10652
                     /gene="nrdE"
                     /locus_tag="PNS21_001998"
     CDS             8493..10652
                     /gene="nrdE"
                     /locus_tag="PNS21_001998"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDI5159702.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10702..11667
                     /gene="nrdF"
                     /locus_tag="PNS21_001999"
     CDS             10702..11667
                     /gene="nrdF"
                     /locus_tag="PNS21_001999"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDI5159703.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11756..12895)
                     /locus_tag="PNS21_002000"
     CDS             complement(11756..12895)
                     /locus_tag="PNS21_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDI5159704.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13204..13917)
                     /locus_tag="PNS21_002001"
     CDS             complement(13204..13917)
                     /locus_tag="PNS21_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDI5159705.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14171..14872)
                     /gene="nagB"
                     /locus_tag="PNS21_002002"
     CDS             complement(14171..14872)
                     /gene="nagB"
                     /locus_tag="PNS21_002002"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDI5159706.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15106..16638
                     /locus_tag="PNS21_002003"
     CDS             15106..16638
                     /locus_tag="PNS21_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDI5159707.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16829..>17006)
                     /locus_tag="PNS21_002004"
     CDS             complement(16829..>17006)
                     /locus_tag="PNS21_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5159708.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2910 g         5323 t
ORIGIN      
        1 gaaaaaacac attccactaa atttgatgcc ggttttcctt gaggttttgt tccagcacct
       61 cctttaattt ttttaagctc ttgatctgaa cattgtttag catttagtct atttttttct
      121 tccattacga ttccccctac aaactataaa tacttttatg tcactgattc aatataccta
      181 tttttctaat caaaaaattt tgagacatat ttaatattat aagtatcatt aaaattgaga
      241 tttatctcca taatctgctc gatataaacc atttattcca gcagttcttc caatttcact
      301 caaatttctt attaattcct cttgatattc agtgaatctt attttattag tataagcagt
      361 aaaatataga taattcacca atctatttat taaaggactg ggatctaatt catatttttg
      421 tttttctaat tttaaatata ccttttgtaa aacctcaaga atatcagaat atttaggatc
      481 tttacttata gatatatcat tatataattc atgaacaatt tgcttagcat tttttttcat
      541 tttagcactt ccctggaatt gctccaccta aaaaaccacc tatagacatt cctgcaatac
      601 aagtagttgc cttggcccaa tcgaccgtac atttattttt agtgcaatac actccatttc
      661 cataatattt tccactatga gtggtacccc catagataag ttgtgtctct ttaatagaca
      721 aaatttttaa atgtttcata atctaatcac ccctaaatta atgtttttag actattattc
      781 taacattcat taaatttcaa aaaacaattc cttcttgaaa cgtagcaaaa taattcctga
      841 ataagaattt aatcactttt aagtatataa actatgtata ttctttaata ttttttgaga
      901 atgcacttcg tacataataa actttaatca cattagtctt tcatacctac gatcgatctc
      961 tttgactcgc acccataaat tcataatagt ttgtcggcgt ctttgaacgt ttcgaagatg
     1021 agctacgcat tctctttttt tatagacaaa aaagatagag acgaacatgg agcttccaaa
     1081 tttggatatt tgatccaatt ttggaagctc catttatttc gtgtctgtct ttttattttt
     1141 tccagctttc gactaattct tggttctctt tgatccagtc ttttgctgct tgctgtggat
     1201 cttttccatt gttgatttcc aacataactt tttccatatc tttttctgac caatggaaat
     1261 tgtctaatac tttgtaagct tctggctggt cttctttcaa tccttttcga accatcgtat
     1321 ggatctgctc ttcgcttccc attccgtttt caggatcttc taagtatttc agatcatatt
     1381 tggcaaacat ccaatgaggt gtccatcctg taactacaat tggttcgtgt tttttgatcg
     1441 cttgacctag tgcaacggtc attgctcctg aagaagatgt ctctactttc cagtctttca
     1501 agttgtcata tttttggatc gtgttttcag cagccgtaac tacaccagct cctggctcga
     1561 ttcctatgat tttcttacct gcctgatcgg ttaactcatc gatcgaattt acatccatgt
     1621 aggctggcac tgctaaacca acttttgctc ctgtcaaatt ggcacctagg tcttcgactt
     1681 gatctttata ttgtgcatat tgtgaaccat gagttttagg tagccaagct gaaaccatgg
     1741 catctgactc gccatttgaa acagatttcc acatgattga attatctaat ggcgtcatcg
     1801 ttacatcata gcccatttgt ttcaatactt caccgactac atttgttgaa gcaacttctg
     1861 tatcccattc aacataagat aagttgatac gttttgtttc tttggcagat gaaaaactcc
     1921 ctataccaat cgcacctaag atcactacta ctacagcacc aatgatcagt ccttgacgtt
     1981 ttttactttt ctgtgttgtt gctcctgcag cttttttatt gtttggctgg ttcaaatgct
     2041 gtgtaaaacg gtcaatgatg attgctaaga taactaatgc aaccccgttg acaaatccat
     2101 tcccgacttg tgctctttgc aatgctgata atacgccgcg tcctaagccg ggtgcaccga
     2161 tcatagaggc aataacgacc attgaaagtg acagcattgt tgtttgattg atccctgcca
     2221 tgatcgtact tttagctaaa ggcaattcta gtttgaatag tttttgacga cttgtgctcc
     2281 cgaatgaatc agaagcttct actaattctt taggaacttg tcgaatccct aaattcgtaa
     2341 aacgaacggt tggtggcaga gcaaagatga ctgaggcaaa tacccctgga accatcccga
     2401 taccgaaaaa tgcaactgct ggaatcaaat aaacgaatcc tggcattgtc tgcataaagt
     2461 caaggatcgg tttgataata ctttgggctt tactgctttt tgccatcaag atccctaatg
     2521 gtaccccaat gatgatcgag atcacactag ataaaagaac aagtgtgaca gtattcatca
     2581 aatcattcca tagaccttgg ttataaataa accatagacc gattaatgtg aacaacgtaa
     2641 gtcctggacg tttcttagaa ataaagaaag ctgctaccgt caataacaag ataaacaaag
     2701 gagctggaat cactaacagt aaatttgtaa tccccgacat cactgcctgt ccaatcgttt
     2761 gtaaaaatga aaataggcca gaaaaagttg tcgtaaacca ttctgtgata ttttctaccc
     2821 agtttgccac tggtaattga tagttcaata gattattcat gttcactcac ctcgcttgtt
     2881 tctgctaatg cttcaatcac actacctcgg atgacgacac cttttaattt tccatcctct
     2941 acaactgcaa gtggagtcgg tgagtcataa ataatattga aaatatcact taccaccatg
     3001 tctttcccaa tcaccgtcac attttcatct aggaagtcga tcaatggacg tttttgcttc
     3061 ctagcttcta acgcttgatc tgcagtgatg atccctttca ggtgtctctt tctgtcaaca
     3121 gccatcagca tgctgacttc ttcattacgc atccttgtca atgcaacgtt tggtccatca
     3181 gattcgatat tcgtcgtcaa tgcaggtacc atgatgtttt gagccgtaag cactttggaa
     3241 cgatcgactt cttccacaaa ttcacgtaca taatcatttg ctggattggt caatatttct
     3301 tcacctgttc cgatctgcat gatttcgccg tctttcatca aggcgattct gtccccaatc
     3361 cgtaaagctt cattcaaatc atgggtaatg aaaataatcg ttttttgaac attggcttgc
     3421 aggtccagta attcgtcttg catttctcgc cggatcagcg gatcaagagc cgagaacgct
     3481 tcgtccatca gcaagatctc aggatcattt gctaaagctc tggctaggcc aacccgttgt
     3541 tgcatcccac cagataactg actaggatat tggtctttaa atgaaagcaa actagaattt
     3601 tctaatgctt tttctgcttt ttcttgtcgt tcttctttag gtacaccacg gacttctaac
     3661 ccgtattctg tattttccaa gatcgttcga tgtggaaaca gaccgaagtt ttggaaaacc
     3721 atattgattt tatgacgacg gacttctcgc aattcttctt tgcttaactt cgatacatct
     3781 tctccatcaa tatagatact gcccgaagtt ggctcaatca gacgattcaa tagacggatc
     3841 aatgtggatt tcccactccc ggaaagcccc atgatcacga agatttctcc ttcttttacg
     3901 tcaaaattca cgtcatacac accaaccgtt gcaccggttt tctttaaaat ctccgttttc
     3961 ggctggtgat cattcatcat atctaaagcg gcttgtgtct ttttaccaaa tatctttgtt
     4021 aaatgtttta cttctacttt taccaaacaa attctccttt tttatgtaaa ttcatttttc
     4081 tttctgaaaa aagtgaccac actatgttaa catcatgtag tcactttgtc aaaaatgaaa
     4141 gaaatttgct tgtccaaact gaaaaaaact tggctaaatc tttgtaaata aaaggtttag
     4201 ccaagttaat atttttttaa tatttcacac aatcattctg caagctgatg ctccgggtaa
     4261 aaaaagtttg tcatacgctg tagtgtatat tcatttccaa taaaatgaac gatgtcccct
     4321 gccgtaaatt tagcataggg gccaggtgaa acaagcagtt cttcattatg agaaattgcg
     4381 ataactgtgg cgccagtcgc ctgccagata ttcaactcgc taattgaacg gtccagatgc
     4441 attgcttttt ctgataaaga taattcaaac ggaagtaaag gattcatctt atgtattcgc
     4501 ttcgtttgat tgaccagctg atccaataat tgagaaaaat gattcagctc atcttgttgc
     4561 ttctccacac ttgccatgat ttctgctttt gtatcttgga tggattgtac atcttgatat
     4621 tgcaatagga aatcttttgc tttttccttc gaagcaacaa atgccccact tccatgccgt
     4681 acttccatga ttccaagatc taccagcaca ttgatagcct tgcgggtagt ctcaggagaa
     4741 gcattgtacg tactggcaag tgttgagcgg gcatggatct tctcacccac tttatatcgg
     4801 ttttcaacga tacgttcggc taaatcaaca gcaatctgct ggtagcgcgg gcggacgatt
     4861 tttttctttc cagacatttt ctttcctctt tcttttttta ctttccctca tcttactatg
     4921 ctctagcaaa ataagcaaaa aaggggagaa tagcctcttg caacgactga tcatcgcttg
     4981 atctgttcct tttttactgg gcaagctgtt tgacagtcat cacaattctt accggatttt
     5041 aaacgagtat agacaattgc gcctgcactt ccaaagatta acacacttaa taagaatgtc
     5101 gccatcctat tcctctcctt ctaatgacaa tatagtataa aactggttct tggattttgg
     5161 ctgacggaac aaaacataaa cgagtcctat catcagtaaa atagcaagat acgtccctaa
     5221 cgctacctgt ccaccctcca ataagtgtcc caactgataa atcacaaaac taactaaata
     5281 agctaaaccg cattgatagc ctatggcgat ccaagtccat ttcttggcat tcatttcccg
     5341 atggatagcc ccaattgccg caaaacaagg cgcacaaagt aaattgaagg ctaacaatga
     5401 ataagctgct gcgggagtat atgtgtgttg tagcacttgc cagatttctc tgccattttc
     5461 ggatacttcc gcatgaccaa acaaaatacc aaatgtgcca ataatatttt ctttagcgac
     5521 caatcctgta atcgctgcga ctgttccttg ccagtttccc caaccaagcg gctgaaaaac
     5581 aactgccagg attcgcccga aaaaggctaa gatactttgg tcttcagaaa caggttgagc
     5641 ataaaaatta taagaagagc tgaaccagat cacgatacta gaaacaaaga taatcgttcc
     5701 tgctttttta acaaaagact tgctgcggtc ataggtttgt cttaggaccg ctgaccattg
     5761 aggaaaatga tatgcgggca attccatgat aaaaggagct gtatctccag caaacaaacg
     5821 tgtttttttc aagctgattc cagagaaaat aattgcagtc ataccaagaa aataggcaga
     5881 aggagctacc caagattgat gaggaaaaaa ggcccctgct actaaggcaa taatcggcaa
     5941 ctttgcagaa caaggcatga aggttgtgac catgatcgtc aaacgacgat cttgctcgtt
     6001 ttcaatcgtg cgactggcca tgacgcctgg cactccacat cctgtagcaa tcagcatagg
     6061 aatgaaggat tttcctgaaa gaccgaattt cctgaaaagt cgatccatga caaaagcgat
     6121 cctcgccata tatccgcagt cttccagaaa gctcaagcat aaaaacaata taatcaattg
     6181 cggtagaaat cctaacactg ctcccacgcc tgcgatgatg ccgttcaaga tcagctcctg
     6241 catccatccc gctacttgcc attcagctaa caatctgcca acatgttctg gtaaccatgt
     6301 accaaacaat gtgtcattga tccaatctgt ccccattgtt cctactgttt gaatagccag
     6361 ataatagatc agccacatca caaatgcgaa gatcggcaag gctaaccaac gattcgttgc
     6421 aatctgatcg attttgtcac tcatcgataa ttggaaagaa tcatttgaca caacacagag
     6481 tgtacatagt tgggtaataa attcataacg ttcattgacc agaatcgtct cactatcgtc
     6541 gtccaatagt ttctctgtta actggatcag ttgctcgatc tctttttgct gagaaactgc
     6601 tatctcgtaa tcctctctta ccttctgatc tcgttcaaaa tatttcattg cataccagcg
     6661 tttcttgccg ctttgttctc ctagtacatc ttcaatttca tgtaaagctg cttctaaacg
     6721 tggatcatat gtacagggaa caggaataga aggccgcttt tttgcagact cctgaatctt
     6781 ttcgattagt ttttcgattc cttgcttttt caatacgctg attggtaaaa ctggtacacc
     6841 aagaccatag gaaagctttt ctagattgat tttttttcct tcttttttga tgacatccat
     6901 catattcaga ccaatgatca taggaatgtc taaatccaat aactgcgtag ttaaataaag
     6961 atttcgttct aaattggtag catcaataat gttgatgatc agttcaggct gttcctgaac
     7021 caaataatct cttgcaatca cttcttctgg tgtataagga gacaaagaat agatgccggg
     7081 taggtcctga atatgaatgt tcggattttt tttcaacgat ccttcttttt tttctactgt
     7141 tacgcccggc caattcccta catattgatt cgaaccagtc aagagattga acaagctagt
     7201 tttcccgctg tttggatttc cgactaacgc gatattcatt ctttttcctc cctttgtatc
     7261 aagattagtt cggcctctga ctttcgtaac gtcagttcat accctcgcac tgtcagttcg
     7321 attggatctc ccagaggggc tagttttcgg ataactattt ttacatttcg cgtcaatccc
     7381 atatccatta accgtcgctt cactgcgcct tgtccgtgga ttgctacgac gatcccagtc
     7441 tctcctgctt ttaattgatc aagagaagtc cagctttcag aggaagcagt ctttttattg
     7501 acaaagattt gacctagtat ttcccgactt actgccactc gatgactttt taccagtaaa
     7561 atggcattgt tgcctgataa attgacgacg acgattttac ttccggcaac gatcccgata
     7621 ttttgcaaat ggcgttttgt tttttcttct gtttcgattc gttcgacttg ataaacgaaa
     7681 ttctgctttg cctgcgctaa ggtctgcata ttgattcctc tcttgtgaaa taaatcatgt
     7741 attaaaatca ttcattatat gttctaattt tcttacgtgt tacgcaaata aatgagaatc
     7801 attctcacat agaatactct cggtaattga gaatgtcaat tatacttgtg atattatgag
     7861 cgaatcttta gtattgatag ataaaaatga gaaaaaaata tttcttcata gttttccaca
     7921 attttttctc tattaaattt gctattttcc cacacgatac ttgttttcag ctcttttttc
     7981 aaaaacacaa aacttagtat tccacagttt ccacaacaca atatgtagtg tttgttgttt
     8041 gactttttta ctaagagttt tatactatct attatagata ataagaatgt gttagaggag
     8101 gcactcacca tgaacgtaaa aatcttttct aaaaataatt gtatgcaatg taagatggtc
     8161 aaacggtttt taacagaaaa tcaaattgct tttgaagaag tcaatatcga tgaacagcct
     8221 gaagcagtag actggttgaa agcacaagga ttccaaagtg ttcctgtcat tacttctgat
     8281 gcagaaacag ttatcggatt ccgtcctgat cagttgcgta aacttgccag ctaacatgac
     8341 taaaagaatc aaaacgtgat acatggattt gaagggcttg agacagcacc agtttttgtc
     8401 gcaaacttcc aaatcctttt ttaaagctta aaaacaatgc aatgatttgc aatcaagcat
     8461 catcaaatac gaaatgaagg ttgggcgtac gtatgagtct taaaaattta caagatgtca
     8521 gttatttcaa gctgaataat gaaatcaatc ggccagtcaa tggtcaaatc cctttgaata
     8581 aggataaaga agctttagtt gccttttttg aggaaaatgt caaaccaaat acgatgactt
     8641 ttccatctat tatggaaaaa atcaattact tgatcgatca agaatatata gaagcagaat
     8701 tcattcaatt gtattcacca tcatttattg aaaagctata tcaatttttg gacgagcaac
     8761 atttttcttt caaatctttt atggctgctt acaaatttta ttctcaatat gcgttgaaaa
     8821 ccaacgacgg ttccgcttat ctggaaactt atgaagatcg agtagccttc aatgcgcttt
     8881 actttgcgaa cggggatgaa gaattagcac ttgctcttgc agacgaaatg atccatcaac
     8941 gatatcagcc agctacacct tctttcttga atgctggacg taaacgtcgg ggcgaattgg
     9001 tgtcttgttt cttgactcaa gtcactgacg atatgaactc tatcgggcgc tcaatcaact
     9061 ctgctttgca attgtcccgt attggtggcg gcgtagggat tactttatca aatcttcgtg
     9121 aagccggcgc tcctatcaaa ggatatgagg gtgctgcaag tggtgtcgtt ccagtcatga
     9181 aattattcga agatagtttc agctattcta atcaattagg tcagcgtcaa ggagctggcg
     9241 tggtttattt gaacgttttc catccagaca ttattgcttt cctttcagca aaaaaagaaa
     9301 atgcagatga aaagattcgc gtaaaaactc tttctcttgg cgtgatcgta cctgataaat
     9361 tttatgaatt gacacgtaaa aacgaagata tgtatttatt tagtccatat agtgtcgaaa
     9421 aagaatatgg tgtcccattc tcttatgtaa atattacagc tgagtatgat catctagttg
     9481 caaatccaaa cattcgcaaa caaaaaatca aagcgcgtga tcttgaaaat gaaatctcaa
     9541 aattacaaca agaatctggc tatccttacg taatcaatat tgatacagcg aaccgcgaga
     9601 atccaatcga tggcaagatc atcatgagta atttatgttc agagatttta caagttcaaa
     9661 cgccttctgt gattaacggc aaacaggaat atgaagtatt aggaacagat atctcatgta
     9721 acttaggctc gacaaatatc gtgaatttga tgcaaagccc taactttggt caatctgtgc
     9781 gtacgatgac tcgtgcattg acatttatta cagatgcatc tgatatcgat gtcgtaccac
     9841 cgatccaaaa cggaaataag ctgaatcata cgattggatt aggtgccatg ggcttacaca
     9901 cttatcttgc aaaagaacaa atggaatatg gctcagagga ttcattagac tttacaaata
     9961 tttacttcat gctgttgaat tattggacac ttgttgaaag caacaatatc gctcgtgaaa
    10021 gaaaacaagt tttccataat tttgaaaaat cagcttatgc tgacggcact tatttcgaaa
    10081 aatacgtaac tggagaattc cagccaaaat cagaaaaagt caaagcattg tttgaaggca
    10141 tttttatccc aactgctgaa gattggaatg ctttgaaaca agcagtaatg aaagatggac
    10201 tgtatcatca aaatcgcttg gcagtagcac caaatggctc aatttcatat atcaacgata
    10261 cgagtgcaag tattcatcct atcactcgca tgatcgaaga acgccaagag aagaaaatcg
    10321 gtaaaattta ttacccagcg ccttatctat ccaatgagac gattccgtat tacacttctg
    10381 cttatgatat ggacatgcgt aaagtaatcg atgtttatgc aacggcacaa caacacgtcg
    10441 atcaaggcat gagtttgact ttattcatgc gttctgaaat cccagaaggc atttatgaat
    10501 ggaagacaac aacgaagcaa acgacacgtg atttgaatat tttaagacat tacgctttcc
    10561 acaaaggaat caaatccatt tattacgtac gtacctttac tgatgatgca gaagaaatcg
    10621 gaagcaatca atgtgaaagc tgcgtgatct aatatcatag atagattcgc ttattcatat
    10681 tttaattaaa ggagcattct catgtcagaa acatattatg aagcaataaa ctggaatgaa
    10741 attgaagata tcattgataa atccacttgg gagaagctga cagagcaatt ttggctagat
    10801 acacgtatcc cactttcaaa tgacctagat gattggcgta cattgtctca attggaaaaa
    10861 gatacagtag gacatgtatt tggcggtttg acactgctag atactgtcca atctgaaagt
    10921 ggcatggatc agttgcgtaa agatgtccgc actccccatg aagaagccgt tttgaacaat
    10981 attcaattca tggaatccgt tcatgccaaa agttattctt ctatttttag tacactgaat
    11041 acgaaaaaag aaatcgaaga aatttttgac tggacaaata cgaatcctta tctgcaaaaa
    11101 aaagctgagc gtatcaatga aatctacaaa cacggtacac cgctagaaaa gaaaattgcc
    11161 agcgtctttt tagaaacatt cttgttctat tcaggttttt atactcctct ttattattta
    11221 ggaaacaaca aattggctaa cgtagcagag atcatcaaac taatcatccg agatgaatct
    11281 gtacatggaa cctacatcgg ttacaaattc caattaggat tcaacgagtt acctgaagca
    11341 gaacaagaaa cgttaaaaga ctggatgtac aatcttcttt atgagcttta cgaaaacgaa
    11401 gaacgttata cagaagaatt atacgatcca atcggctgga cagaagaagt caagacattc
    11461 ctacgctata atgccaacaa agcattgatg aacctaggga tggatccact ctttccagat
    11521 actgccaatg atgtcaaccc aatcgtaatg aacgggattt caacaggcac aagcaaccat
    11581 gacttcttct cacaagtcgg aaacggctat ttgttaggtc atgtagaagc gatgaaagac
    11641 gacgattatt tgattggatt ggaataagga tttattttaa ttcaaaaatt tttcgaaaaa
    11701 taaaaagagt ttagcgtaca gatattttaa atgtacactg aactcttttt tatgcttatt
    11761 cacttgtttc ttccagcact tttggactgt ctaacttttc cgaaacatgt tccattcttc
    11821 tcaataaata gattttccgg atatttaata ttatcgtctt aatcactgca tagacaggta
    11881 tgcaaatcag catgcctaat attcctgcga cacttcctgc tccaatcaat accaagatga
    11941 tcgtcaaagg atggatattc attgatttcc caaataacaa cggcttgatc aaatttccat
    12001 ctaattgttg gatcactaaa atcgacaacg ccatatacaa agcttgaaat ggagagataa
    12061 acaaaccgac aatgattgct ggtaccgctc caataaaagg gccgacatac gggataatat
    12121 tcgtgattcc gcaaatgacc gcaagcaaca agctataagg ctgatcgaat attctcattg
    12181 ccaaaaagct cattacacca ataatcaaag catctaagat cgtactgctg atataggcag
    12241 ataatgtttc gtttaattct tttaccgttt ggcgtaactc tttacgaatc gctttcggaa
    12301 agaagtgaga caacgcatca ataaatttgt gcccatcctt gaacataaat agcaaaataa
    12361 atggaacggt aaataacaaa ataaaaaatt gcatcataaa tgaaatgatt tttgagacac
    12421 ttgcagataa actgactaat atgatattga acaaattact aagagataaa tttgctgatg
    12481 ctaattgttc ttccaaattg aaatttttaa actcttcccg ctgcgccaat tcatttaacc
    12541 atttgcttgt ttcatctgca tacattggca agctctgggt caattgaatt gtctgttcca
    12601 ttaactgagg cactacattc atgacagtta aaacaactaa aatgattacg accaaaaacg
    12661 tcaataaaaa accgaacact ctcgggatcc gatgcttttc gagaaaaaca acgattggat
    12721 cgaacatata atagataaaa ccagcaacaa gcaatggtaa aaaaattgcc gaaagcattt
    12781 tcaatattgg actaaaaata ttcggcattt gtaaaataaa gtacacgccg atgatcgtga
    12841 gtactagctc cactgtccag aaaaataatt ttgattgttt aaaacgtgat tgcatacatc
    12901 tcctccaagg tcctttgata cttctattct atcatttaac aaagagttac gcgctgagat
    12961 tttcatttgc ttaaaaaaaa caaaaaaaat aaacatattt taatgagtag acgagtactt
    13021 aaacaaaaac acggtttgga gtagtgacaa aaattatact gcagtccctt tttccaaata
    13081 aacggtgttc aaaagcttgc atcttcgtaa taagcccagc caaacgaaaa atataaggaa
    13141 ctatttttat ttggctgggc ttattacttg atgtaggaca tctattttac aacctcagat
    13201 catttattgc gcaatactga ctaaatgcaa ctttgtgata tttttgtgta cggacaactc
    13261 ctcgatcaca tcggcataag tgagcccttt tggaagatca atcgtataga tatttttgta
    13321 cactcggtag tcatcaacga attttacgtc aaaattcaca tcttcgatta cgatattttt
    13381 ctcttcaaaa taatcggtaa gaaaatcttt ggtttctttt ctatggataa attggatttc
    13441 taacttttta gtcgtaggta catggatcac gcgtttcact actgtgagtg caatagcaat
    13501 cccgataaaa ctgatgatcg caatggaata gtaacccatg ccgatggcga tccctaatcc
    13561 agcaattgcc cataaagaag cggcagtcgt aagtcccgtc accgattgtt tcgtgacgat
    13621 gatcgttccc gcgcctaaga aaccgacacc actcaccact tgagcaatca gccttgcttc
    13681 atctgaacga atcgttccag caagctgtgg atactctaaa gcatctctta atgcaccact
    13741 agcgatctca acttggatca atgcaatgat acatgctccc atacaaacta aaatatgcgt
    13801 acgcatacct gctggacgat ttttatattg tcgttcaaaa ccgattgctc cgccaaatag
    13861 catagcgatc cctaaacgta aaataatctc tggaattgtc aataccatat cattcatcta
    13921 aacaccctcc agtccaatat atcatgtctg aaaggagcag acaaaaaaag tgcaattttt
    13981 acctcttacg aaaaaaggac tgtgacaaaa tttttgtcat agtccttttt atgaataaac
    14041 ggtgttcaat cagctgaccc tcggtattac tcaacaaacg gcaaaatatg gggagctatt
    14101 tttcctttgt cttctctaat actcaggtca aaacgctgat ttcacaacct ctttagccta
    14161 ctattgaaaa ttacaactta cttgctgctt cttcatcgat gatcacaaca acattgtcgt
    14221 gattttgcaa tacacttgct ggcaaatgat tcgttacagg tccttcaatc atccctttga
    14281 ttgcatcagc tttgctttcg ccaaatgcca tcaagacgat ttctttactt ttcataattg
    14341 aaccgattcc cattgaaaca gcttttgttg gtacatcttc tgctttatcg aagaatcgtt
    14401 tgtttgcatt gattgttgat tctgttaagt caactactga agtcgttccg tcaaatgaag
    14461 caccaggttc attgaaaccg atatgtccat tttgcccgat tcctaagatt tggatatcga
    14521 ttggatgtgc atcgatgatt tgatcatact ttttgcatgc tgcttccaga tcttctgctt
    14581 ttccatctgg tacaaacgtt tctttgaatg gttttttatc aaataattgg acatccatga
    14641 aatgacggta gctttgttca tctgaaccgc ctagtccaac gtactcatct aagtttacag
    14701 aagtcatttc actgaaatcc aaatcgcttg aagtcatttc tttatataaa gtaactggtg
    14761 tgcttcctgt tgccaatcct aatacttttg cacctttgtt cataccttct ttgatgattt
    14821 caaatgcttt ttttccgcct tcttcagcgt tttttacacg aatgatttcc atagtgattg
    14881 ctcctttata ttttggtata gcccaattat agcattcttt ttttcttttt acaataatta
    14941 gtatagacca aattgcattt ttttcatttt cttgacaata aataggaatc cgtgttaggt
    15001 ttcataagat ggttcaatca aacaatccat gttgcatgaa aaccaagcaa gtgattcact
    15061 gtggacgctt tatgtctgca gtttttttat gaaaaaggag ttattatgag tatactgaca
    15121 ttaaacaaca ttacccagca atttggcgac aaaatcctct atgaaggtgt cgatctgcaa
    15181 ttaaatgctg gcgaacatct tgggctgatt ggacaaaacg gagccggtaa aagtacattg
    15241 attaaaatca tcactggtga gatcttacca gatgacggac agatccattg gcaaaaaaat
    15301 attcacaaag gttacctcga tcaatatgtg gaagtcgatg aaacattaac aatcgaagaa
    15361 tttctaaaaa ctgcgtacgc aaaagaatac gaaaaggaag aaaaaattgc ttttctttac
    15421 caaacatata gcgagacctt cgatgattcc ttgctggaaa aagctgggaa actccaaacg
    15481 gaattagacc aaggagtgtt ttatcaaatc gacaccttag ttgctgagat gagcagcggg
    15541 ttggggatcg atgtcttagg tttagattca ctgttgaaaa atctaagtgg tggacaacgt
    15601 tcaaaggtga ttttagcaaa actactttta gaaaacccag atgttctgat cttagatgaa
    15661 ccaaccaatc acttagatga tcagcatatc caatggctga cgcagtttct tcaggatttt
    15721 gacggtgcgt atatcgtgat ttcccatgac caagaatttc ttgatcagat caccacccat
    15781 atcgcagaca tcgagtttgg aaagattact aagtatactg gccatctaaa acaagcattg
    15841 aaacaaaagg aacaaaatcg cgaaagctat ttgcgccaat atcatgctca acaaaaacaa
    15901 atcgaaaaga ccgaagctta tattcgaaaa tacaaagccg gttcccgatc aacgatggca
    15961 aaaagccgac aaaaacaatt agataaaatc gaacggctga cgcctccagc ttcagcaagt
    16021 aaagctgtct ttgattttcc attctcgcct attgtcacga cattagcagt ggaaacaact
    16081 gatttagtca tcggatatga tcgaccgcta cttgcaccca tcaatttgac catccgcttt
    16141 ggtgaaaata ttgccattcg aggcttcaac gggattggaa aatctacttt attaaaaaca
    16201 ctgatcggtg agattgaaaa aataagtggc gatttccact tcccagataa cacaaaaatc
    16261 aattactttt cacaagatct gtactgggaa aatccattag aaacaccact acaatattta
    16321 agcagtcaat ttccaaaagc gactatcaaa gaattacgtc gccaactagc taaagcaggg
    16381 ttagttaatc aattagcaag tgaacctctc actacattaa gtggtgggga acaaactaag
    16441 gtgaaacttg cacagttgac aatgaaccaa ggtaatttac tgattctcga tgaaccgacg
    16501 aaccatatcg atcaagaaac gaaagaaagc ttacaagacg ggatacagaa atttccagga
    16561 acagtaatca tcgtctctca tgaacaagaa ttttatcaag atcttgtgca acgagtcatt
    16621 gagattgaac caaaataaac gtttgattga atagttgaaa aaaaggagct gtgacaaaag
    16681 ttttgtcaca actccttctt ctgaataaat ggtggccaat tagctgacct tcggtattag
    16741 ctcaaaaaac ggcaaaatat gaggagctat ttttatttgg ctattcttat tacttgatgc
    16801 agaacagctt ttcacaacct cttttcgtct attttttatt ttcgagattt ctctttcctt
    16861 taaaaacaac tgtttgaaag aagaacgcac atccttgaaa aacaagcccg ctaaccgtta
    16921 cacctaacca gccatactgc tgccacattg tcgtccctag caatgaacca gccgcacctc
    16981 caataaagta cataaacata aataca
//
LOCUS       DAMYXM010000053        16711 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.054, whole genome shotgun sequence.
ACCESSION   DAMYXM010000053 DAMYXM010000000
VERSION     DAMYXM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.054"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..1372
                     /locus_tag="PNS21_002005"
     CDS             <1..1372
                     /locus_tag="PNS21_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDI5159709.1"
                     /translation="VDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTY
                     PDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVT
                     DVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTD
                     LNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVT
                     VTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNK
                     KGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLP
                     DGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNG
                     SKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKN
                     RKKENE"
     gene            1506..2849
                     /locus_tag="PNS21_002006"
     CDS             1506..2849
                     /locus_tag="PNS21_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDI5159710.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2961..4412
                     /locus_tag="PNS21_002007"
     CDS             2961..4412
                     /locus_tag="PNS21_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDI5159711.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            4761..5393
                     /locus_tag="PNS21_002008"
     CDS             4761..5393
                     /locus_tag="PNS21_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HDI5159712.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            5981..7420
                     /locus_tag="PNS21_002009"
     CDS             5981..7420
                     /locus_tag="PNS21_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5159713.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            7715..7933
                     /locus_tag="PNS21_002010"
                     /pseudo
     CDS             7715..7933
                     /locus_tag="PNS21_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8182..8397
                     /locus_tag="PNS21_002011"
     CDS             8182..8397
                     /locus_tag="PNS21_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159714.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8614..8870)
                     /locus_tag="PNS21_002012"
                     /pseudo
     CDS             complement(8614..8870)
                     /locus_tag="PNS21_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9057..9968)
                     /locus_tag="PNS21_002013"
     CDS             complement(9057..9968)
                     /locus_tag="PNS21_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDI5159715.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(9987..10991)
                     /locus_tag="PNS21_002014"
     CDS             complement(9987..10991)
                     /locus_tag="PNS21_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDI5159716.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(10988..13111)
                     /locus_tag="PNS21_002015"
     CDS             complement(10988..13111)
                     /locus_tag="PNS21_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDI5159717.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(13116..15563)
                     /locus_tag="PNS21_002016"
     CDS             complement(13116..15563)
                     /locus_tag="PNS21_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDI5159718.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(15550..15939)
                     /locus_tag="PNS21_002017"
     CDS             complement(15550..15939)
                     /locus_tag="PNS21_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDI5159719.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(15999..16502)
                     /locus_tag="PNS21_002018"
     CDS             complement(15999..16502)
                     /locus_tag="PNS21_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDI5159720.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(16515..16676)
                     /locus_tag="PNS21_002019"
     CDS             complement(16515..16676)
                     /locus_tag="PNS21_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159721.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
BASE COUNT         5401 a         3114 c         3077 g         5119 t
ORIGIN      
        1 agtagatcta acagataatg ttactaactt gccagcgtta ccagaaggaa caacagtaac
       61 agatgttact cccggcggta caattgatac caacacacca ggtaattacg aaggtgtcat
      121 tgaagtaacg tatccagacg gtacgaaaga tacggtgaaa gttccagtag aagtgacaga
      181 caatcgttct gacgccgata aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg
      241 aggcacagta gatttaacag ataatgttac taacttgcca gcgttaccag aaggaacaac
      301 agtaacagat gttactcccg gcggtacaat tgataccaac acaccaggca attatgaagg
      361 tgtcattgaa gtaacgtatc cagacggtac gaaagatacg gtgaaagttc cagtagaagt
      421 gacagacaat cgttctaatg cagataaata caatcctgaa ggacaaaaag tgactacaga
      481 cttaaataaa gagccagatg catctgaggg aattaagaac aaagaagatt taccaaaagg
      541 aactacttat gcttggaaag agaaagtaga tgttagcaca gctggaaata aaaaaggtac
      601 tattgtagtg acatatccag atggatcatc tgatgaagtt gaagtagacg ttacagtgac
      661 agatactcgt tccgatgcag ataaatataa tcctgaagga caaaaagtga ctacagactt
      721 aaataaagag ccagatgcat ctgagggaat taagaacaaa gaagatctac caaaaggaac
      781 tacttatact tggaaagaaa aagtagatgt tagtacagct ggaaacaaaa aaggtactgt
      841 agtagtaaca tatccagatg gatctaaaga agaagtagaa gttactattt ctgtagtaga
      901 taaaaaagca ccgaataaac ctcaagttga tcctattaca gagggtgacc aaattgttac
      961 tggtaaaact gaaccaaatg cagaggtgac agttacatta ccagacggaa gtcaacacca
     1021 tgggacagct gataaaaatg gtaattttac agttaaagtt cctaaattag aggcagggac
     1081 aaaagtgata gtaactgcaa ctgatgaatc tggtaatact agtgaaccta ctaatgtagt
     1141 tgtttcttcc aacaaaaaag acagtggaaa aaacggtagt aaaggtagta agactgacaa
     1201 tcaaggtagc aacagcaatc aagacaaaaa tagaggcaaa agccagtcct caaaagtttt
     1261 tcctaagacg ggagagagtg acagtaatat ctttactatt agcggtggtt taatactgtt
     1321 aggaacgtta ggattattag ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa
     1381 tttcttaagt cttctctgag aagaggaaga atctttttct tcctcttttt gcttttttat
     1441 gatgggaaat aagtgttagt taaagatgaa tatacttttt atagaaagga aaagaatgta
     1501 attaagtgga aaaaatagta attattggtt caaaccattc agggctagcc gcagctcgat
     1561 ttctaaactt aagcgattca aattatgaag ttattctaat cgataaaaaa tcagaaatta
     1621 gttacttgga atgtgggaca tcgttattta taaaaggaga agtaagtaat ttagaagatc
     1681 ttttttatca aaatagaatg acactaaatt ctgagataaa aaagttattt ctagagacag
     1741 aagttgacaa aattgatatt caaaataaaa agattagttg tgtaaaaaaa gatggtacaa
     1801 gattctctca agaatatgac aaagttatat tagctacagg ttcaaagcaa attatgctag
     1861 atataccggg gaataattta gctaatattt tttcgataaa gaattttcca aatgcaaaag
     1921 agatttttaa agctttggat gatagtacta ttaaaaaagt aggaattatt ggagcaggat
     1981 atattggagt tgaaattgct gaagccataa gaaaaagagg gaaagaagta tatcttttcg
     2041 atgttgcaga tagagtgttg tcgacttatt acgaccgtag tttttcagat aaagttgaag
     2101 aaatactatc aaaaaatgga atacatcttt gtctatcaga aagtcctaaa aaatatttag
     2161 gaagaaaaaa gattgaaaaa atagtcacta caagtggtac ccaatactct atggatatgg
     2221 tcgtacaagc tataggattt ctatctaata gtccaattgg ggaaaaggaa ctcttgagag
     2281 atgttagtgg agcatatcta actaatgaat accagcaaac aaatataaaa gatatatatg
     2341 ctattgggga ttgtgctaca acttttttta cgtccattaa tagaagtagt gttgattttt
     2401 ctatttctaa tgctctaaga acagcttata ttgcaactca acatattaac aatcaagatt
     2461 ttacaccttc tggttcccaa tttacaaatg gcttttctat atttaattac aatttttacg
     2521 cagcaggttt gaatttaaaa acagcacgat tattaagtga gtctatagat tatatagaaa
     2581 tagaagattt cattagacct gcttttttga gaaaaaatag tcgtgtaaaa ttaagaatta
     2641 gctttgaaaa aaatacaaag agaatagtag gtgtacaact ttgtagtcaa gaagatttat
     2701 ctggactatt atcaatgttt tctcttgcta tagaagaaca aattacatta gacaaattga
     2761 aaatgttaga ttatttattt catcctagtt ttagccaacc ctataatttt attttaaagg
     2821 cagtaacagg attaaacatt gaaacataat cgttactaaa tgaaatcaag aaatatactg
     2881 tattactcaa ttcattgaaa atatttatca taatttttaa tagaaaacac tattaaagag
     2941 aaaaaagaga ggtaaatcaa atgaaaaaat ctttaaaact tgcatcagca gttatattaa
     3001 tgtcccaact ccttatagca acacctttcc atgtgttggc aagcgaaaat tcaacataca
     3061 aaattgcaaa agaaagaacc gagacgactg aaatgagtaa ttccgatgta acagcagaga
     3121 gtagtgaact tttgacaact tcatcaagtg aaagtagtac gacttcaaat gccgagtctt
     3181 caacaacttc atcaagtgaa agtagtacga cttcaaatgc cgagtcttca acaacttcat
     3241 caagtgaaag tagtacgact tcaaatgccg agtcttcaac aacttcgtca agtgaaagta
     3301 gtacgatttc aagtaccgaa tcttcaacaa cttcaagtag tacaagtgaa ataacttctt
     3361 cgaaagtgac aaaaccaaac catatagaat cttcaaaaaa ggaagcgcca aaaacggaag
     3421 gacaaacaaa aactttttcg acaccacagc aaatagaatc aacagaatct ttccaatcac
     3481 aaccaatact atctggtaat gaagaaattc attttgacaa aaatgaatct gtagaaaatt
     3541 ttattatgaa aattggtgag tctgcgagaa aaattggtca gaaatatgat ttatatgctt
     3601 ctgttatgat tgcacaagcg atcttagaat ctgccagtgg tcaaagccag ttagctcagg
     3661 caccgaatta caatttattt gggataaaag gaacttataa tggaaacttt gtcattatgg
     3721 taacaaacga agatttgggt aatggaacgc tttatacgac ccaatcaaaa tttagagtgt
     3781 atgaaaacta tgaggaaagc tttgaagatt atgccaaatt attaactaag ggaattagtg
     3841 gaaataaaga tttttatgca ggagcattaa aagctaatag taaaacatat cgagaagcaa
     3901 ctaaattcct tacaggacgg tatgcaacag atacacaata ctatttgaag ctaaatgaat
     3961 taattaaaac ctacgattta accaactatg ataaagaagt taaaggtcca caaatgagta
     4021 agactggata ccaagtgcct attcataatt attcaataac cagtttgttt ggaaatcgtg
     4081 gaaatgaatt tcaccgagga cttgatatgg cagctacaca gggtgagccg attcatgcag
     4141 ttaaagctgg taaggttgtt aaagcagagt ttcactactc ttggggaaat tacgtggtga
     4201 tacaacacga agatggatca accgcgttat atgctcacca gcaacaatac ctagtaaaaa
     4261 ctggagataa tgttttgcaa ggacaaataa ttggctatgt aggaagtact gggaatagta
     4321 ctggcccaca ccttcatttg gaaatttgca aagatagtag tctttctcag agcatgttag
     4381 ttgatccgaa aaccttatta ttcggagact aaaaaaggta atcaatctga caaagtaaca
     4441 agaaaatggt tggattctta gacctttgat caggatattg agactggaat tgatatctcg
     4501 attacgatgg gtttgacaaa taaaacaagt ccattatcga atatcgagtg tattattgcc
     4561 gtctttatgc tcatattttg agcaggtttc actagatgaa aacaatttat ctatcttgag
     4621 gatttcgtgt acgtatcgtt cagtcttgta ttgtaaatta gtcataaaat cagactaaga
     4681 aaaattggaa atattttttt ctgatttatt aaaagttaat tgaaattata tggtacttct
     4741 ttcttgggag ggtaggtgca gtgaaggaaa gatatggtca ccttactgtt gttgaggaga
     4801 ttactcataa tggatataaa aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg
     4861 tatatcgatc aaacttacta tcaggtagaa ccaaaagttg tggatgtgga aattataaaa
     4921 ataggcataa atataaaaat ttgacaaata aaacttttgg taagttagtt gcaatcaagc
     4981 caactaaact gcgaagtgaa ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa
     5041 aaaagttagt atcggcaaag cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga
     5101 aatcaaaaaa gcatcacatt gaaaataaaa aattcggatt acttactgca aagtatcctt
     5161 tggacaataa ctgttcatgg aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt
     5221 gtgagataag ttatagtaac ttattttttg gacatactca aagttgtgga tgtttaaaaa
     5281 ggcaagaaac aaggacatgt gttgaaggga cagtaataga gtgtctaacg tcaaaattac
     5341 cgaaaaataa tacaagtggt gttaagggag tttatcgttc taaagagaaa tagattgcgt
     5401 atataacttt aaaaaaaaag aggtactatt taggatcgta tgattctatt aagagtgcag
     5461 ctcaagctag aaaagatgct gaaaataaat tgtttcaacc tttgattgac aaatatcgag
     5521 gtaatcatta attattgtgg ataataatta acagtgatat ataaacatct ataatatatt
     5581 attcatacta ttgatattta gtataatcct atggaattga atatcatagc aatgtatttg
     5641 aaaagcaaat tggaaaacaa ataggtgcaa caccataaat atgaaaaaat tgggaaaaag
     5701 tagaatggaa atttgcttac gtctcacgag atagctcaca ctattaatct gaagaacaat
     5761 gaagtcgact tttagaaatt gctagaagaa agtaaagcga attatcacta gttactatag
     5821 agtaagttca aataaagcat ctccaactta catggttaca tgaggatatc aaatcgaaat
     5881 tactcaagct tcctatggct agagtcgcga gaagttgttg ggcagtagac ataggttata
     5941 ttgttcttag ctacacgatc attagaaagg aaatcctatt atgtatagtt atcaagaaga
     6001 tgcaaaagtc caaaaaaatc gctggtggat tttgattgct atatctatgt ttaccttcat
     6061 gtccacattg gatggcagta ttgtcaatat tgcactacct accatctcag atgatatggg
     6121 cataccaatg aatcaagcgg aatgggtagt gtccgtttat ctgatgattg tctgttcttg
     6181 tttgttgctg tttggtaaaa ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg
     6241 tacgctgatc tttgtcttgg gatcgctgtt gtgtgggttc aatcattcgc taggtatttt
     6301 attgttcgca cgggtggtac aagccattgg tgctagtatg actatggcta caaatgccgg
     6361 aatcatcacg gaagtctttc ccatacatga acgtggtcgg gcattgggag caataggtgc
     6421 gtttgtttca ttaggctcga ttgctggacc aggtcttggc ggcttgattt tgtcccaatt
     6481 ttcttgggcg tacatttttt ggatcaatgt accggtggga atcgtaacga tgttgattgc
     6541 tgaaaaattc ttaccaaaag atatcaccat gagcggccat acaatcgatg gagggggctt
     6601 tgctgccatt gcggctacga ttatcagctt ttttggagca atctttttgg ggcaagaaag
     6661 tggctttttc catccagtcc cccttgtact gttcgtaatt tcagtgattg cctttgtcag
     6721 ctttttaaaa gttgaaaaga aagcagcctc tccattgatt tctttcggta tttttaagaa
     6781 caagattttt accatgagct tgattacagc cttgttgatt ttttcttcca acttctttgt
     6841 caatgtagtg attccttttt atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg
     6901 gctgttgatg atggtctttc ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct
     6961 gaccgaccgg gttggcccgg agattttggt attagtaggc ttgggcttat tgtcggtaac
     7021 ccaggtcatg tatatgtttt tgtcagtgga ctctcctttg tggctataca ttgtcgctac
     7081 tgccatcatg ggtgccggca atgccttgtt ccagtccccc aataatacga tggttatgag
     7141 ctcggtcact atcgaaaact tgggactggc aggctcgatg aattcctttg cccgaaattt
     7201 ggggatggtc agcgggattt cgctttctac gacggtctta tatcaagcga tgtctgctaa
     7261 gatgggccag caagtaacta actatatcgc aaatcggccg gatgtctttt tatatggaat
     7321 gaagattacc tttttaggca gctttgtttt gtgttttgtt gcctttttcc tgactatctg
     7381 gcgtatcaaa aaacaaggag gtattcagaa aaatgtctga aaaaaccact gttgaagaat
     7441 ttttggaggc ctgcgtatgg gtgagtggga tttccatgga tgatggaaga tgaagtgaac
     7501 ttctaaaagc cgaaaaactc aagtattatc ttcaaaatac tagtgctcaa tgagatgctt
     7561 gatttgatca caagaagact gcgaaagccg ctggcaaccg caagctaggt atcccaccga
     7621 tggactacgt tttagaggct actggttatg aatacggagc caacgctctc atctgcataa
     7681 gaatacatca cccagattat gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat
     7741 agataaatga tttccgtcta gtcaaattgg ttcagaatgt agacacacag atggtaagaa
     7801 gtcactcaca attcgagaat ggacttgtcc tatttgccgt atacatcata atcgaaacgt
     7861 caacgatagt atcaatattt tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca
     7921 tcaagaacct taggaacaac ggggatagct tggttaataa gagataccgc tgtttaggct
     7981 tgcaagtata ctcttttatc aagaattccg tggctttagt catgagaggc ttaatactat
     8041 gaaaattttc tttagcattt ttgatttagg ttccttttct aattataata agatgaccac
     8101 tacagtaacc tactgaaata tataaacagc cactgtagta ccattttcaa agtagtctat
     8161 gagaggagaa tcttcattct tatgaacgct ggaattgcgg atatgaattt aataaaaaaa
     8221 acccttaatg attttacttc caattcaata tcaaaaggta ctggtatcaa tttaagcaca
     8281 ataaaaaagc tgaaaagtgg tgaaagatca gtggaaaagc tgaatctttt agatgctata
     8341 aaaataacgg aatttgctat gaaaaatgga aaagctgaga ttgaaatatg gagataatta
     8401 tcgtctagtg aattacttgc cacttagctg aatttaacga tccttggtgc ttgaaggggg
     8461 agattcttgg gaatagagca tacttgcaag cctatgtctt tactaacaac gatatctctt
     8521 attaaccaag ctatccccgt agttctaagg cttttacttt tatctaagca ataatcgtat
     8581 tcaaaggaaa tttttgaccc ttagaaagtc tcattttatc agacgtgagc ttaggcattg
     8641 ctgaattttg ttaattcggt tttaggttgt gctttgagca taatatgaaa gtattcttta
     8701 tcacgatttc attcatctaa ggtaatgtga tgtgattctg aaattctttc aaaggcagtt
     8761 tttgcataag ctgacattac atcatcaatg acatgtttac aatgtttcac tactaaaata
     8821 agggggtaac caagaagaaa aactgaatga ttatttgtat ctaattccat ggtatatcaa
     8881 ccatttccta tatagactga ttatgacatg gattaaaata aaaaggcact atgtcttaat
     8941 tgtcggtttt cgagttaccg aatggcattc atcacccacc tatagaggat gggcgacttc
     9001 tgcctgaaat aagttacact acttataact tctaataaaa ttagattgag tcattattat
     9061 ttaacatcaa cgattttcca gtttccattt ttttgtaata ctaatttgta ctgaaagtag
     9121 ctagtggttt ttgatacatt atcaaggtat tttatctttg tactcactag aaaatttttt
     9181 ccgttttgta gataaactgg attctctaac tccgaaaact ttaagtcaat attgattggt
     9241 cgcatcgcac cattttcaac ataatactcc aattcttttt cagaagctga tggataaagt
     9301 ttaaaaaatg tttctaaaaa atcggtgatt tcttccgttg ttttggcatc aatttctgaa
     9361 tcgttttcta cttgttttgc ttcatatttt gccttgtctg gctttccaat cattgttggc
     9421 aaactagtta ctaaatgatt cctgttttca tcttcaaaga ttgtcactcg atatgcagaa
     9481 gaaattgatt gcgtcttttt tccctctgta atatgttgct ccactaaaaa tgaaacgaca
     9541 aattcttccg agtgtttttc aacatctaga atttttacag attgaacttc ggaactcgtg
     9601 ggaatatctg ctcgaaccat atcttggatt aaagcgagac cgttttctgt aaggtactgt
     9661 tcgagattac tcattctctt ttcaattgct tctttgttat ttttccatga aaaatattct
     9721 ttcgcaaaat cctctgtaaa actttcaacg ccagaagtat tcactacctt ctcttttata
     9781 accacttttt catgagttgt atgttgatca atggcggtga aatttttata aacgccaaaa
     9841 gcaagactac aagacaataa aagccaaagg gcaagcacca tttttcgatg cttacccacg
     9901 gatacaactc gttctttttt cttttttggg ctcttttcct tttgattccg ttcaattttt
     9961 atcttcattc tttttcctct tttctcttat ttgtatcggc cagctccgat aagatgctgc
    10021 cgccaatagt tttccgttag attcgtccac cccactggat ctcctgcatg aaacatgcga
    10081 ttctcccctg cgtaaatgcc cacatgggtc acataagtac cggcatcata ggttgaatga
    10141 aagaaaacta aatcacctgg tttggcatct tttagatcaa tatgagtcat ggcatcatat
    10201 tgatcctgag ccacccgagg aagtttcagg tcaactttgg cataacacca ttgggtcaag
    10261 ccactacaat caaaccccgt ttctggactt gagccaccaa acacataggc cgttccttca
    10321 tacttaaatg cctcatcaaa aatttcttgg actattttgt catcaaactg agttgtagtt
    10381 aggtattgct tcactaataa acagtaaaac atattgccat aattataccg ccacccacca
    10441 ttttccttaa tcgcgattgg gttggggtaa tcgacttttt gaccacctga tttttccttt
    10501 gaaaaagatt cagctaaagc aaaactatgc ttcttcccgt ttttcccgac atgatctaga
    10561 tacccgccac catagttata ggcctgaata atactatctt gatcacaccc tagttctttg
    10621 gaacgcttaa ccaaagacgc aaaatagaga cagccttgtt tgatggatgc ctcggtactt
    10681 aatgaattag gtggtaatcc ggctgactca gaactttgca taacgtcttc cattgtgcca
    10741 ccagattcca cttgaataat cgccaaaaga attggaatat actctgttat atccgattct
    10801 ttggcatact tttccaccat tgcacggtgt ttcaatactt cttctgagac actagttccg
    10861 ccaggagaag tgccagaact accatttccc tcactgtctt caccaaacaa caaacccaca
    10921 cacatcaaaa tacccataaa agagatggtc agaaaagtta atgaaataac acccactttc
    10981 ttcaagttca ttttttctca cctgaccttg attttggacg atggttcatt cttttttgta
    11041 ctttttgatt ggtctgcttt ggcagagtgg acctctgttt cacttgttgg aaagaatttt
    11101 tcttacttgc tgattggctt ttcccttttg aaatgatttc ttgactagtt agttttcgtt
    11161 tgatttcggg attcagattt gctctcacaa tccgctttgg tgcttctttt ttcagcatca
    11221 tatctttctg aggttgagct gctttatcta ttggaatatt cgcccttctt tttccctgac
    11281 gatttgttgc ttcactcagt atcttgcgtt tttcgtccat tttcgcattt cttcgttgtc
    11341 gttgtttttc tcgcaaatcc actcgatccg ctttttcttg gaacagtccg cgcttgaatt
    11401 cttctggtgc ttttttcgac ttttcgattc ctttatggag attgtatttc aaattcgttg
    11461 gcgtattcct tacctgatcc tttacttgtt tagtcttatc tttcaccaaa gcttgggtat
    11521 ccaaaacttt gccgattttt ctcccagcca aattcattcg ggattgtttc ttattatttg
    11581 aaactggatt ctccttagtt aagtcagata gtatttcgtg atcgttcggt aatcgtgaat
    11641 tctttcgtag agaagaaccg gatggcttaa atttggattt tgattgttcc tttgccaact
    11701 tatttccgac tgctgcacca ccaccaaaag ccaaagctcg tcccacattt cgattgagtt
    11761 gccgcaattt tcgattcatt aacatttgcg gttttcgcat gacgcgtctt cccattgact
    11821 ggctatcatt actctggagg ttgaacatac tcataatgtc gcccaatttg aaatagatac
    11881 cggcaaaggt cacaatttgc agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat
    11941 tgaaaaacat cgtggagatg ctaaatgcag tcgtaatcac caaagtaaca cccgctcgca
    12001 tcataatcgt attgaataaa cgaataatcg ccttctttcc taaactttca taagtcggca
    12061 acatggacaa taagaaacta attggcagaa acatcgcaaa gataatgaag agaatttgcg
    12121 agaacaacat aatccctgtt agaagaaaaa caaagaagga aatgccaata ttgaacaata
    12181 ccaaaaagac cacggtccct aaacggttca tggtcttggt aatactgaga ttcgcattgt
    12241 cattgtcttc aatctctgcc ttgaccgctt cttcccgatc tttgcctttg ttttcatctg
    12301 gactcacaga tagaatcttg ttgacccggt cttcaccaat ttcttctatg ttggaatcat
    12361 caaattgcag tagtaaccaa ggttgttgga cttgaatcga aaacaaacta tcccgaatca
    12421 aatccacact atcttttcct tggctttctg aattgggtac aacgatctcg gttccaaggg
    12481 taagggccgc ttcactaaca tctgagctaa agtcattgat ttttgtgata taggtaggcg
    12541 cataagcaat gaacgaaccc gatagtagaa aaatcatcac aaaattaatc acggctcgaa
    12601 ctgctttcgt ggtttctcgt tttaataatc cggtatacgc cacatacacc cccagagcta
    12661 aaatcatcaa taatagaaac ccaaaataaa atccggaggt ctggagtcca ttttcagtaa
    12721 taccagccaa tgtttggata ttctttccaa tactttcagc cgtatccgaa atgaaatcta
    12781 atttgtaagc ctcttgaacc acataacccg tggcgttcga caaatacaaa ctcaccgtcc
    12841 aaatgaaatt agtaatggca taaagaccat acatcacgct tttccctaag ccatcacccc
    12901 aattccaagg caaccaatcc caagagctat ccacaaagaa atctagttga tagttgttca
    12961 gtgaatattt ggaatataga tttcctgctt caactgtatc atcaactagc cctgtcgcct
    13021 cggctaccgt accaactaaa ctcaatagaa ctatcagagt gagtatgcta ataaaaacga
    13081 ttcccacaat ccgaaaaagt ttctttttca tcccctcact ccattctctc ttgaacaggt
    13141 ggacgggtat caaaggcatg gaagagttct tcaaagactg gatgtacctg aaccacgcct
    13201 acccgaccat acaaatcctg catcaagcat tggccatttt ccaaatctcg taggcgtttt
    13261 tgattgcttt catcttctcg atccaagcca aagaattcca aggttttctt gatttcatga
    13321 atatcggtac tgcgaaacgc aaacttcaac ccgatattat tcttcaagcg ttcatccagt
    13381 aaatcatcac tattttgcgt gacaaagtaa accccggcat tcatagcccg cccagcacga
    13441 acgagcttat tcgataatgt tttcccttgc gctacttgaa gaaaactcca cgcctcatct
    13501 aagtctacaa ttttgaagat ttctcgatta ctatggataa agtccaaggc aaaagtggaa
    13561 atgacgatta gcatggaaac actaagcaat tccatggtgg tatattcttc aaaagtggtt
    13621 tctgcatctg gtaaaaccaa atccgctact tgaatgatat ttaactgcct atctaaacta
    13681 atcgactggg taatcgaacc atctgagaaa agcaaatggg caaaatcata atcggtaaag
    13741 gactcgatgt gatccgcgat attttccgca actggtgagc cttcttttcg aagctcatta
    13801 attacttgta ataatccccg ttgattgctt tgggtaaccg tccgaatggc cttacgcaac
    13861 actggaaact tctccccatc tcgactagaa attcctgtta aaaaggtcag aatatcaatt
    13921 gctagacttt cagaatcctt ctttttctcc ataatcacat aaggatcaag taatccttga
    13981 ttttttggct tactagttaa gttgagaata ttgatttcat ccgcaatttc tggcaaggtt
    14041 tctttccagc caccccgttc cccttttgga tcgacaatca cagcttgtcc accaaaaagg
    14101 acggcgtaat aaactaagag attgttacta aaggactttc cgccaccgag tgaaccgagg
    14161 aatgccgctg ctagcgcatt ggtaactgaa cccttgaccc cttgtgaggc caatgccggt
    14221 ttcaggtaaa cattgcgccc cgtatctacg ttataaccaa agtaaatgcc ttccaactcc
    14281 cctaacatct gagtggcgcc aaaaccaagt ccggcgagaa aatcagacgt aacatactgg
    14341 atataatcat tcatgtatcg cttactagaa ggtaagaatt cttcatgtag ccccatcata
    14401 tcgccaaaag gccggactag ttttacattg gtatcatcat aaaaatctag cacttcatca
    14461 caccggcgct ttagttcatc tactgattcg gcacttactc gaaccacata gctcagtttg
    14521 tacatggatt ctttggactg atccaaggta gtttccaatt catctacgga atccaatgca
    14581 tctaagacat tggaatttgt ttcattgtca gattgatagg catggttgtc caagtctttt
    14641 aattcctttt tcttattgcg aacggtagca agtgcctttt tgttcgtcac aatctccaca
    14701 ttcatactag tgtcaattgg gaatgtaaat tgttgttgct ggtaatagaa aagttctgag
    14761 gagggaaact ccatttcccc cacaattgta ttaattgtta agtacgctac atatgattca
    14821 tgattctcat gttccatgca taagtaccgg ggcttttctt caatcaaaca acgactggga
    14881 cgtaataagt cgtaacgttt caccaaagtt tccgacttca gctttttctt tggtagctga
    14941 aattcatatt cttcaaatgg tgtacccttt tcgccataga tatgttcaat caaatacatc
    15001 agatcactcg gtgtgactcg tctgacttta aatcttcgag ccaatttact ttccattagt
    15061 ttttcaagct tggtatatcg gcgaatttct tcattggata aggaaacaaa atcgcccatc
    15121 aaatgatgat tcactccata gacaaattct tgaaagccgg agaagaaaga tttcttgaga
    15181 ttctttagat tgacctcttc atctgtcgca atcagtttga agccaataaa aaagcgataa
    15241 tcaatctgac tatcgccaat catggatact aatgcctctg tctgtaaatc aattcgttgt
    15301 ttcgctactt ctgttaatcg cccggtaatt tccttttttg atcgttcttg cgtggcacga
    15361 atactacttt ctgtggcaat ttgtaaggca tgaatcttgc cttcacgatt ttgcgcaatc
    15421 aattgacgaa agttatcgtg gacttgatat ttctgttcag gtgacaaaaa ggagtaattg
    15481 taaggtatta actcatagta ggcaaaacat tctccctctt gattaaagac aagattattt
    15541 tcaatatact taatcgggta catagttcac actccttacc agagtaatgg gttcgtccca
    15601 tcgtttcttc tgttctttga ctttctttcc tgcgaaagtg accttaaatc gtaagtgata
    15661 gctaataaaa gatcgtaaaa atcccaatgg tttcttaccg tcaaacgttt tttgcgacat
    15721 gaaccaagta actgccaccg gtatacctaa gtattttaaa aaggccccct ctatcataga
    15781 tagtggaggc acatgggcaa atacgatgac aaacagcaag gataaaacaa accaagccat
    15841 ctggttaaaa gtcacaggaa aaggcagttg aaagtcatta attgcataaa tgaccttttc
    15901 aactgcccaa atactggtgt agctttttat tttcttcatc aaatcattcc tttaaaaaga
    15961 aggatagttt agaaatcaac tacccttcaa taattttgtt aatatggata ctcaaaaaca
    16021 cctcgactcg ttaccaaata attgcctcca atctccaagt ctctaccaca cgcatcaaaa
    16081 tcaatatacg tcttcaagtg ttctggtact tctccaaaat caccagcttc tacaatcaag
    16141 aaatatgcta catcactcat atcatcgcaa tctggataat agataatatc atccacatgc
    16201 tctaccatct cctcaaaaga attgaagaaa gcatgttgaa tctcagaagc aacttcacca
    16261 actggcgttc cttcaagttc ctccgctaag ccacacaacc gattaatttc ctcaattggc
    16321 gtatactcat caatctcaaa gggaagttca taatcatgaa ttgcatattc ctcgtattcg
    16381 tcatttaacc caattcgttc tttcacttca tcaaaatcga caggtggggt aaaccacgcc
    16441 cccaccaatt caccttcgtt atatttcccg aggttcgcaa tataaacacg catctgttcc
    16501 atcgtgcttc ccccttacgc accgacaatt cggttaaaca aactgagtaa gacatccttc
    16561 accccagaag cattaaagac caatccgacc gcaatcaaag caatcaccaa aaagccaatc
    16621 agtttggaaa actcccgctt aaatcctaaa aacaaaccaa tgaccacaat tgccagtaag
    16681 actaaggatt gggcattggt taaaaaccat t
//
LOCUS       DAMYXM010000054        16670 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.055, whole genome shotgun sequence.
ACCESSION   DAMYXM010000054 DAMYXM010000000
VERSION     DAMYXM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.055"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(63..701)
                     /gene="plsY"
                     /locus_tag="PNS21_002020"
     CDS             complement(63..701)
                     /gene="plsY"
                     /locus_tag="PNS21_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDI5159722.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            828..1262
                     /locus_tag="PNS21_002021"
     CDS             828..1262
                     /locus_tag="PNS21_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDI5159723.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1428..3473
                     /gene="parE"
                     /locus_tag="PNS21_002022"
     CDS             1428..3473
                     /gene="parE"
                     /locus_tag="PNS21_002022"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDI5159724.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3485..5935
                     /gene="parC"
                     /locus_tag="PNS21_002023"
     CDS             3485..5935
                     /gene="parC"
                     /locus_tag="PNS21_002023"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDI5159725.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6212..8443
                     /gene="pflB"
                     /locus_tag="PNS21_002024"
     CDS             6212..8443
                     /gene="pflB"
                     /locus_tag="PNS21_002024"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDI5159726.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8687..9448
                     /gene="pflA"
                     /locus_tag="PNS21_002025"
     CDS             8687..9448
                     /gene="pflA"
                     /locus_tag="PNS21_002025"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDI5159727.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9533..10459
                     /locus_tag="PNS21_002026"
     CDS             9533..10459
                     /locus_tag="PNS21_002026"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDI5159728.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10631..11524
                     /locus_tag="PNS21_002027"
     CDS             10631..11524
                     /locus_tag="PNS21_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HDI5159729.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11658..11783
                     /locus_tag="PNS21_002028"
     CDS             11658..11783
                     /locus_tag="PNS21_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5159730.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11917..12549)
                     /locus_tag="PNS21_002029"
     CDS             complement(11917..12549)
                     /locus_tag="PNS21_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159731.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(12697..13239)
                     /locus_tag="PNS21_002030"
     CDS             complement(12697..13239)
                     /locus_tag="PNS21_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDI5159732.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13374..13763
                     /locus_tag="PNS21_002031"
     CDS             13374..13763
                     /locus_tag="PNS21_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159733.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13767..14531
                     /locus_tag="PNS21_002032"
     CDS             13767..14531
                     /locus_tag="PNS21_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDI5159734.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            14660..15607
                     /locus_tag="PNS21_002033"
     CDS             14660..15607
                     /locus_tag="PNS21_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI5159735.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            15737..16477
                     /locus_tag="PNS21_002034"
     CDS             15737..16477
                     /locus_tag="PNS21_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159736.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         5887 a         2818 c         3460 g         4505 t
ORIGIN      
        1 aataagggag tgtgacatcg atttgtcaca ctcccgtttt atctctttgg aatgaaaaag
       61 tatcaggatt ttttcttgct gctattcaaa ccaaacggga ttcgactttc agtatggttt
      121 ctgatacgct tgatattgtc tttatgccga taaaagataa aggaagaaac taaaaaggca
      181 atgatcgtca gtaaccaatt gggcttagcc aaaatcacag gagctacata aggaaggacg
      241 atcgtagata aagtgatcaa tactgcactg atcatactgc tcaaactcac catacttgta
      301 caaaataaac taacgataaa gatgatacaa gaataaacaa aaaatacagg attaaatcct
      361 aaaagcattc ctgcacttgt cgcaacagct tttcctcctt tgaacccagc aaaaatcgga
      421 aatgtatggc caagaatggc gcatacccca aaccaaagag ggttaatccc agaaaggtgg
      481 aagatcatcg gcaaacaagt agccaatgta ccttttaaaa tatccataag caggacaaca
      541 atcccggctg ttttgcctaa tacacgaaat gtattggtcg ttccagtatt accgctcccg
      601 tgttgtcgga tatctttttt gaaaaacaat ttgccaatcc agacaccgga aggaatagaa
      661 cctaaaagat atgctaaaat aagtaataag atcaatttca ttttttaccc tctttcaagt
      721 tgaaaactat cctttatttt agcatgaata ggtaacagga acaattgaaa aaatgaaaga
      781 tacaagtata atagatggaa taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa
      841 atccaacaca agaaacgatt ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag
      901 gcttgagcaa taaaccagaa cgaacaagtt acatggttgc acaggtattg caagacaatg
      961 gatataaaat cataccagta aatccattat tggctggtga agaaattcta ggcgagaaag
     1021 tctatgcgaa tttgacagaa atcgatgatc caatcgatat cgtagatgta tttagacgga
     1081 gtgaattttt acctgaaata gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg
     1141 cacaattagg gattgaaaac aaagaagcag ctgagatgct aagaaaagca ggacgtacag
     1201 atatcgtaat ggatcgttgt attaagattg aacttggtaa gttgaaagaa aaccaaaaat
     1261 aaaaatgacc acaaagaaga gatgatcgat agaggggaat gtcgaaaaag cggttaggag
     1321 ataattaaaa ttgatttctc gtctaccctc ttttttttca cgaccttttc tagtatgata
     1381 atcgtggtgc ggttttgccg caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag
     1441 taaacaacga atacaatgac tcgtccatcc aagtattgga aggattagag gcagttagaa
     1501 aaagaccagg gatgtacatt ggatccactg acagccgagg cttgcatcat ttagtctatg
     1561 aaatcgtcga taatgcagtt gacgaagcat tatcaggtta tggcagtgag attgatgtga
     1621 ccatccatga agacaatagt ataactgttg cagatagcgg acgtgggatg cctgtaggaa
     1681 tgcatgcttc cggaatacca acagttgaag tgatttttac tgtgctgcat gctggcggaa
     1741 agtttggtca aggagggtac aaaacttctg gtggactgca cggtgtagga gccagtgtgg
     1801 taaatgcctt atcgaaatgg ctaattgtaa cgatcgttag agatggcgta gagtatcagc
     1861 agaaatttaa aaacggcggt aaacctgacg gtactttgaa aaagatagga aaaacaaaaa
     1921 aagcaaacgg gacgactgtc cattttttac cagatgatac aatattcagt acaacgaaat
     1981 tttcatatga aattttagcc gaacggttaa gagaatctgc ctttcttcta aaaggcgtta
     2041 aaatctctct ttctgatctt cgtggagaag aaccagtcaa ggaaattttt cattacgaag
     2101 aaggaatcaa agaatttgtt gattatctga atgaagaaaa agatacattg acaccagttg
     2161 tttatttttc aggagaaaaa gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg
     2221 gttattcaga aaatgtttta tcttttgtca ataacgttcg gacaaaagat gggggaaccc
     2281 atgaagctgg tatgaaagcg gcgatgacga aatcttataa tgaatatgcg agaaaagttg
     2341 gtttgctgaa agaacgggac aagaatctgg aaggtagtga tttcagagaa gggttagctg
     2401 ctgtactttc tatccgtgta cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa
     2461 aacttggtac gccagttgct cgtaccgtag tagataacgt catcagtgaa caaatgggct
     2521 tttatttgca ggaaaatagc gaaatgagcc agatgttggt ccgtaaagca atcaaagcca
     2581 gagaagcgag agaagcagca cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta
     2641 aaaaaggcga atctcttctt tcaggaaaat tgacacccgc acaatcccga aaccctaaga
     2701 aaaatgaatt gtatttagtc gaaggggatt ctgctggcgg atctgctaaa caaggaagag
     2761 acagaaagtt ccaagcaatt ctacctttac gaggaaaagt catcaatacc gaaaaagcta
     2821 aaatgcaaga tatcttgaaa aatgaagaaa tcaatacgat gatctatacg attggtgcag
     2881 gagtcggtcc tgagttttct atcgaagact gtaattacga caaagtaatc atcatgaccg
     2941 atgcggatac agacggcgcc catattcaag tattactgct tactttcttt tatcgttata
     3001 tgaaaccgct gattgaagct gggaaagtat atatcgcgtt accgccttta tataaagtat
     3061 ccaaaggaca aggaaagaag caagtcattg aatatgcgtg gacagatgac gaattggcag
     3121 caatgattaa aaaagtcggc aaaggatata tgctgcagcg atataaagga cttggagaga
     3181 tgaatgcgga acaattgtgg gaaacaacga tggatccgac ttcccgtacg ttgatccgcg
     3241 tccgtatcga tgatgctgcg caagctgaac gtcgagtaac cacactgatg ggtgacaaag
     3301 tagaacctcg aagaaaatgg atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca
     3361 gtatcttaga taaaaaagag gatactgaaa tctctccttc agtttctaat gatttattag
     3421 atgaagaacg agcagataaa aatgaaaaca atcagttatt tgaagtagaa taggaggtca
     3481 cctgatggaa aatcgacaag aagtacaaga attaacttta gaagaagtga tgggtgaccg
     3541 cttcggacgt tattccaaat atattatcca agaacgtgca ctgcctgata tccgtgatgg
     3601 attgaagcca gtgcaacgcc ggatattatt tgccatgaac aaagatggca atacgtatga
     3661 caaaggattc cgtaaatcag caaaatctgt cggaaatatc atggggaatt atcatcctca
     3721 tggtgacatc agtatttatg aagcgatggt ccgcatgagc caagactgga agttgcggga
     3781 agtactgatc gaaatgcacg ggaataacgg gagtatggac ggtgatccac ctgctgcgat
     3841 gcgttatact gaagccagat tatccaagtt aagtggagag atgctagcag atatcgagaa
     3901 agaaacagtc gatctcgtgt ggaattttga tgatacagaa aaagaaccaa cagtccttcc
     3961 agctcgatac ccaaaccttc tcgtcaatgg atcgacaggt atatctgctg gatatgcaac
     4021 agaaatcccg actcataact tagctgaagt gatcgatggc acaatctata tgatcgatca
     4081 tccacaagcg agccttgaaa aattgatgga atatattcct ggacctgact ttccaacagg
     4141 agggatcttg caaggaaaag atgaaatcaa aaaagcttat gaaacaggac gaggaaaagt
     4201 tattcttcgt tcgaaaacaa agatagaggc aatgaagggt aataaacagc aaatcgtgat
     4261 cacagaaatc ccatatgaag taaataaagc gaccttggtg aaaaaaatgg atgagatccg
     4321 tttgaataag aaaatcgatg gaatagctga agtacgagat gaaagtgata gaacaggtct
     4381 gcaaattgtc gtcgaattga aaaaagacgc caatgctcaa ggaattttga attatttgtt
     4441 caaaaacact gagctacaga tcaattacaa tttcaatatg gtcgctatcg accatatgac
     4501 tccgcatcaa gttggactga aagacatttt aagaagttat atcgagcatc gaaagcaagt
     4561 cataacaaaa cgtagtcaat tcgatttagc caaagctcaa aaaagacagc atatcgtaga
     4621 aggattgatg aaagctttat cgattctgga tgaagtaatc gcaacgattc gtgaaagtaa
     4681 agacaaaaaa gacgccaaaa agaatttagt cgacgtcttt caatttacag aagaacaagc
     4741 agaagcaatt gtcacattgc aattataccg tttgacgaac acggatatca cagagcttca
     4801 aaaggaatcc gaaagcttga ttgcgcaaat cacagagttg aataaaatcc tttcaaatga
     4861 caaagagctt ttctctgtta tgaaaaaaga attgcgagaa gtgaaaaaaa attattcttc
     4921 cgctcgtcta acgacaatcg aagacgaaat cgaagaaatc aaaattgata cgcaagtctt
     4981 agtggcacaa gaagacgtaa ttgtcagtgt gacgcgagaa ggctatgtga agcgaacaag
     5041 ccttcgttct tatagtgctt ccaaaccaga agaaatcggg atgcgagaag gcgactactt
     5101 gctatactca ggtgaattga gtacattgga ccatgttctt ctgatcacaa ataaagcaaa
     5161 tgtgatctac cgccctgtcc atgaactacc cgatttgaaa tggaaagatg ctggtgagca
     5221 catttctcaa acaatctcca atctgtctgt agacgaatcg attttagctg ttttcccata
     5281 tcaaaaaatt caagcagaga aaacatttgt attcatcagc aagaatggat tgatcaagca
     5341 aacacgaatg acagagttcg aaccatggcg cacctataag agccgtccgc tcagctgtat
     5401 gaagctaaag gcggaagaag atgaactgat tgctgtgtat ctgaaagaag atcagccagg
     5461 cttagatgtt ttcttagtaa cgcatcaagg aatgggtttg cgctatcctt taacagatgt
     5521 tcccgttgtt ggagcaaagg cagctggagt gaagtctatg aatttgaaag atggagatag
     5581 gattatcaac gggctgcttg tgttagcaga aggtgatacg ccgatcgtaa tcgttactca
     5641 gcgaggagct gttaaacgca tgcttgctca agaaatcagc caaacttcac gtgcgaagcg
     5701 gggagtgacg gtcatgcgtg agctgaaaaa aaatccgcac cgtattatct atatgtcgga
     5761 aggaagatca aaagaaataa ccttgatgaa tcaaaaaggg cagcagttaa cgattgaccc
     5821 aactgatttc cctataggtg atcgaacatc gaatggatca tttgcgatgg atgagaaaaa
     5881 aggtggagaa gtcatcgaag ttatcgatgc tcctgagatt ttcatcgagt cgtaactgtt
     5941 gtagtatttt aatgaagaga cgccatctgt attaatacag gtggcgtctc tttttcattc
     6001 atttttaata aatccttata cgtttgcaaa aaaagatgaa agtgttcaca atgcttaaat
     6061 gttggtatgt caacgttgat ttaaatatat gtaaataaaa cataaaaatt gttaaataaa
     6121 aaacaagaaa acccttgcaa agatagggtg ctgtgttata ctaatcacgt aaaaactgtt
     6181 acataaaaca gaaatttagg aggtccatac aatggagcaa tggaatggat ttaaaggtac
     6241 caaatggaaa gaaggcgtag acactagaga ctttattcaa aataactaca ctgaatatcg
     6301 cggtgatgac agctttttgg aaggcccaac agaagcaaca gaaaaattat gggatgaatt
     6361 gcaaaagtta aatgatatcc aattcgagcg taacggtgtc tatgatatgg acacagaaat
     6421 cgtatcaact attacttcac acgatgcagg ctatttggat aaagatttgg aaaaaatcgt
     6481 cggtctacag acagacaaac ctttgaaaca agcattcatg ccgttcggcg gaatcaatat
     6541 ggctaacaat gcgctaaaaa gcaatggcta tacaccaaac gatgaattga cacatatttt
     6601 cactgactgg cgcaaaacac ataatcaagg tgtatttgac gcttatactg ctgaaatgcg
     6661 tgctgctcgt aaaaacaaaa tcattacagg gcttccagac gcttacggtc gcggacgtat
     6721 catcggtgac tatcgtcgtg tggcattgta tggaatcgac tatttgatgg aacaaaagaa
     6781 aaaagatcat gatatgactg gttatcatac aatgagtgaa gaagtgattc gtttacgtga
     6841 agaaatcact gaacaatatc gtgcattagc gcaaatgaaa gaaatggctg cgaaatatgg
     6901 ctatgatatt tctaaaccag ctgcaaatgc aaaagaagct gtccaatggc tatacttcgg
     6961 atatttagca gcaatcaaat cacaaaatgg tgctgcgatg tcaatcggac gtatctcagc
     7021 attccttgat atctatatcc aacgtgattt ggataatgga gtaatcacag aaaaagaagc
     7081 acaagaattg atcgaccact tagtaatgaa attacgtatg gtcaaattcg cgcgtacacc
     7141 tgaatataac caattattct caggattccc tatctgggct actttatcca ttgcaggtat
     7201 gggattagat ggtcgttcat tagttacaaa gaatgatttc cgtctgcttc atacattaac
     7261 aaatatggga ccttcaccag aaccaaactt gactgttttg tattctgaac accttccaga
     7321 aggattcaga acatatgcta gcagaatcgc tatcgaaagt tcttcgattc aattcgaaaa
     7381 tgataatcag cttcgtgaac aatggggttc tgatgattgt gcgattgctt gctgtgtatc
     7441 aggaacagtt gtcggaaaag acatgcaatt ctttggcgct cgtgctaact tggctaaaac
     7501 actattatat gccatcaatg gcggagtcga tgaagtgact ggcgcccaag ttggaccaga
     7561 atatcgtcca atcacatcag aaatactaga ctttgaagaa gtgaaacata acttcatgaa
     7621 tatgatggat tggttagcag aactatatgt caatacgttg aacatcattc attatatgca
     7681 tgataaatat tcttatgaag cagctcagct ggctttgatg gattctgact tgcaacgcac
     7741 atttgctaca ggtattgctg ggatctctca tgcggcagac agcttgtcag cgatcaaata
     7801 tgcaaaagta aaagcaatcc gtaatgaaaa aggaatagca gttgattttg aagtggatgg
     7861 cgacttccct aaatacggaa atgatgatga ccgtgcagat gaattagttt catggatctt
     7921 ggaatacttc atgggacaaa tcaaacgtca taaaacttac cgcaactcaa aaccaacaac
     7981 ttcattattg acgattactt caaatgttgt ttacggtaaa aatacaggaa atactcctga
     8041 tggccgtgaa gctggaaaac cacttgcgcc aggagcaaat ccaagttaca atgcggaaca
     8101 aagtgggtta ttagctagcc tgaactcaac agctaaaatg aattacggct gtgcattgga
     8161 tggaatttca aatacccaaa caatcaatcc ttcaggtctt ggaaaagaac aagatacaag
     8221 aatcgaaaac ttgcgtaatg tcttagatgg atacttctca aaaggagctt atcatttgaa
     8281 tgtcaatgta ttcagtaatg atttattgcg tgatgcaatg gaaaatccaa tgaaatatcc
     8341 gaacttgacg atccgggtat ctggttatgc agttaaattc cgtgacttga caagagaaca
     8401 acaattagat gtcttgatga gaacttcaca cgacagaatg taaaaagttc gtgaaaaaag
     8461 tttttcgact tagcgtcgaa aggacgttga atagccatcg catcaaaatc gaataatcag
     8521 tatgcttatt tgaaagaaac gatttacagg ggcaggactt gtatgttccg tccctgtaaa
     8581 ttttacacat aacgaagcgg caattgcgaa aaattttgtt cagaaagctt cgcttcgaca
     8641 gctaacttgt acttaaaatg aaatcaaaga aaagatgtga acttaaatgg aagaaaaaac
     8701 aatcggctat gttcattcta tcgaaacttt cggttcagta gacggacctg gactgcgctt
     8761 tgtcgtgttt atgcaaggat gccgcatgcg ttgccaattt tgtcacaatc cagatacttg
     8821 gaatattggc ggagggaaag aatacacagc agatgaactt ttagataaag cagaacgctt
     8881 cagaccttac tggggagata aaggtggtat tactgttagt ggtggagaac ctctattgca
     8941 gatcgatttt ttgatcgaac tatttaaaaa agcaaaagag cggaagatgc atactacatt
     9001 ggatacatgc ggaaaacctt ttacgtatga agaacctttc ttttcacgtt ttcaagaatt
     9061 gatgaagtat acagatttac tgttatttga catcaaacac atcgataatg aagagcataa
     9121 aaaactaacg cattgggaca atgaaaatat tttagaaatg gctcaatatt tgtcaaaaat
     9181 caacaaaccg gtttggattc gccatgtact agttcctgag cgaagtgatt atgatgaata
     9241 tttgatccga ctagacaact ttattcaaac cttgtcaaat gtagacagag tagagattct
     9301 tccttatcat acgatgggaa aatacaagtg ggaaacactt ggtttgaaat atccattaga
     9361 agggatcgaa ccacctacaa aagaacgtgt cgaaaatgct aaacgattgc ttcatacaga
     9421 agagtataca aaatatttaa cgcgataggt cgcatcggaa agaagataag tattgcttct
     9481 ttccgttttt ttgctattat taaataggag aaatcacaaa ggagcgaatg acatgtcaaa
     9541 aattttagtt tttggtcacc aaaatccaga tacagatgca atcggggcag ccattgcgtt
     9601 ttcacacttg caaaatgaat taggtaaaga aacagaagca gttgctctag gtacacctaa
     9661 tgaagaaaca caatacgcat tagaccactt tcagctatct gcaccacgag tagtagagtc
     9721 agctaaagca gaaaatgcag atgtcatgct tgtggatcat aatgaattcc aacaaagtat
     9781 ttcagatatc agcgaagtaa atattttagc tgtagtagac caccatcgta ttgcaaactt
     9841 tgaaacagct aatccattgt actatcgtgc agaaccagtt ggatgtacaa gtacgattat
     9901 tttaaaacta tataaagaaa accaagtaac aattcctaaa gaaatcgcag gaatcatgct
     9961 ctcagctatc atttcagata cattgttgtt caaatcacca acatgtacgc ctgaagatga
    10021 agcagctgca aaagaacttg cagcaatcgc tgaagtcgat ctaaacactt atggacttga
    10081 aatgttgaaa gcaggaacaa atctaagtga taaatcagct gcggttcttc ttgatttgga
    10141 tgcaaaaagt ttcccaatgg gcagttccaa cgttcgtatc gctcaagtca atacagtgga
    10201 ccttaatgaa gtgatggatc gacaagcaga gctagaagca gcaatggaag atgaaatgaa
    10261 agcaaatggc tacgatctgt tcgtattagt tgtcacaaac atcttagaca gtgattcaga
    10321 attacttgtt gctggtaaag gaaaagaaaa agtagaagaa gctttccaaa caactttgac
    10381 caacaaccgt gctttgctta aaggtgtcgt ttcacgtaaa aaacaagtcg tacctcaatt
    10441 gacggctgta tttagctaat caaatggtag tatgaagcat tcaaaatgaa tggataagcg
    10501 attgatataa aaagaaacat agaacatact aaaggccgaa gaaatggagg agaataacag
    10561 tcaattatga ctcttgttct tcctcctttt tactttaagt aaaataaaac ggatagaaaa
    10621 gagaacatat atgaactatt attcaaaaaa taaggaaaac ttttatcaaa aactgaccga
    10681 tgatccgctt ttttcttcat tgaccgatta tctatatgaa catcgagaac aagaaacgat
    10741 tctaagagaa ttgaaaaaag aattttcgca aaataaattt tcccattttc ttgatttatt
    10801 gatcgatgca ggattaatca agagggaaga acgaagatat catttgaact ttccaatatt
    10861 tgatcctaaa gattacttgc aacaagccac ttcggctgct gaaaccatag ctgaacaatt
    10921 gaagagactc tctgttgatg aacaaaaatt agcgatggga gaagttattt gggcgtattg
    10981 tttcgaagat gaacggaaag aagcgtattt ttatggagta agaaacagcc gagagacaga
    11041 attgctgagg acaactgcag gaaatgagaa gtaccgtttt atcacacttt cctctataga
    11101 acacttcccg cttactttgg ctaattactt ttttgtacag aagaatcagc ttcctgtaac
    11161 gaaggcattc aaagaattag ccgaacttat tggagacgta aatgaatcgt acttttttga
    11221 tcaaatagaa gttatcgttg acaggatacg taagaataaa tataaaaatc gccggcctag
    11281 tattttccat cagtctctac ttgtgacgaa tactataaag gaagaagaat cattcacatt
    11341 agaattacct attgtcgaaa aaaacaattt tgagattgaa ttaccaacat tggatccttc
    11401 attgacaatg gaggaaatgg cctttcttaa acgacaaatc ttttctgagc tgagcaagaa
    11461 atttataccg catgcatttt cttatatcaa agaatatgga acagttttag tgagtaaaac
    11521 ctaagagagc agtaagcaaa aattatactg actcttgcag gaggagcctt tctcttcctt
    11581 tatggttaaa agaattattt gagtattttg cctatctacc acctattaga ttcactacat
    11641 ggtctggaaa caaaggaatg caaaaaaatg gtgagaaatg tggaatgaca aaggaagtag
    11701 tgataagaaa agttagattt ttaaataatc aatattatga ttcagtcaag tatggcattt
    11761 tatgggaaga actagctgat tagattagga agtccatttg ataatccaga atcactaaga
    11821 aacgaatggc gagtcattaa taatttatcc aatcttgggt tattctttta gattgaagtg
    11881 tttaaaacta aaaaagcatt tatgaagtcg taatagtcag gcttcaaaat gctttttatt
    11941 ttgtttgttt tttctttgcc acataaatac acctagactg acgatgaaga gcatatccaa
    12001 cagatttcct gcgacttgga tcgtcgaagg gttggtcagt ggcattaaag caaggacaaa
    12061 aaaggaaaac aagctgtaaa taaaacaggt gacaggaact aatcgaaaga agagcggaca
    12121 tttcaattgg ctaaaaatga gtgaaaaaat aaaataagta aaaatggata aaatcacgac
    12181 taaaccattc atgataaaga agaaaagcgt attgtgatta aacgaataaa aaagaattgc
    12241 taaaaaagaa aacgttttaa atgcaatcaa tacgcttgaa gcttcgttta acgtaccagg
    12301 atgtcgatga aaaaaatagg caccgaaata aacagaaaac agtaagccgc aaagagaaga
    12361 caaaatcaaa aaaaacggat tttcttcggc ttctgtgata tcagaatgat ccaaaagcaa
    12421 ccagcctaat gcactagaag gcgcatgtga ggtcttaagg tatgaataac atagaaaaaa
    12481 taagatcgtc aaagttgcaa aaaaagagcc taaatgtaaa aaattagtgg ttctacgttg
    12541 aatttccata atatctccaa attaaattga taagtatata aagtataata acaaattgat
    12601 gagtatatgt cgatgttttt taatcaaaat aataaataaa aactgtctaa catttaaaat
    12661 ttaatttaga tattagacag tttttaagtt tgtgagttat tttccttgtc gttttctcac
    12721 ttttttagtc cagaaaccac ctgaaatata ttttggctca taagaaatga taaaagcacg
    12781 ctcttcgatt tctttgatca ggcgatacag ttcacgttcg ctttttctag gagaaagtat
    12841 ttcgagaact agccgctcac cttccagccc cattgcagaa gtctgtgtca caccataacc
    12901 gtgttctctc agtatacccg gcaaactttt atcttctgcg ctatttgctg gcaaaatcgt
    12961 tgtgaccatg atgtatccta atgccaattt gtcttcaatc aaaattccga cactgatacc
    13021 tactgcgtaa cctaacgcat agacaaataa attcagtgga ttatctaaac gatctaaaac
    13081 aagggataag ccgacgatat aaatcgtgat ctcgaccata ctgacaagtg gagcaatcag
    13141 tcgatagcct ttcattgtca acatgaaacg taacgtgttc agagtgatat aagcaaaatt
    13201 aatgaggaag atcatcccca acatttttgg atcaatcatt tgtaaaactc ctttcattta
    13261 tataacaaca atatcaaaaa aaatcgtaag tgaagaaaaa ttgaaacttt atacatacaa
    13321 aaaaatgata aaatgattat aatagaatta taaagatagg ttaggtgatt aaaatgaaaa
    13381 cggtcaatcg tgtgttttgt gccagccacg tgctaagtct ccttctcagt atatacttag
    13441 gtgtctattc gattcaacag ccaattgagg tagagaacat tgtttttcag atgtcgctga
    13501 cagatggttt aattttaagc gtttttttct ttttgatact gtttattggt aatattttcg
    13561 gtagcgttgc cagttttttg aatttttcta tttatccact cctttcgcta gcattaggag
    13621 tggtgggact ttgttctctg tgcttatttc ccgagccatt cagtcctgca tttatattat
    13681 ttggtacact gaacctgttc cagacaactg ttggagcatg gttgctatgg cgatcaggaa
    13741 atttaatgaa aataggtgag taacaattga atccaattat attgaaacgt tctgtccatc
    13801 atattccagt gttagaagtt gttctgaaag aattaagaga ggaacgtatt cccgttgtca
    13861 tctattatca cggatggcag acaagtaagg agttagtttt gacgcaagga agaaaaattg
    13921 cgcagcaggg cattcgtgtg atcctgccag atgcgatgaa tcatggggag cgcaaacagc
    13981 cggtttcttc tattccatcc tatacatttt gggatagtat ctacggaaat ctgtttgaat
    14041 ttgacacact tatcaagcat ttagaaaaaa gaggtcttct acatgatcag cttgcagttg
    14101 gcggggtttc gatgggggga atgacaacat gcgccttgtt ggctaaacat cctcatatag
    14161 tagcgggcgt gtgtttgatg ggttcccctg cacctttgga atatggaaaa gaaatcgtaa
    14221 ggcgcgcatc ggaatataat tatgtcgtac caaaagacta ttttgatttg atttcatggg
    14281 ttaagagcta tgatttgtca ttgcagccgg aaaagttagc tggacgtcct ctgttctttt
    14341 ggcatggaca agctgacgaa aaaatccctt atatacaaac ggaacgattt gtagaaaaaa
    14401 atcggcataa gtcttacgga aaagaaattg tttttcgttc ctcaaaaaca cagcgacata
    14461 tggtacaaat accactaatg gaagaagctt ccactttttt agcaaaaaat ttgataaaaa
    14521 agtataaata attgaaacgg aaaatcactc atgacgatag aggattttcc gttttttatt
    14581 ttgggctata ataaactctg ttaggaaaat ttggtataat tgatagtaag aatgacaaca
    14641 tgttagaaag gaagagagca tgcccacttt agcagatgtt gcaaaacgtg ccaatgtttc
    14701 aaaaatgacg gtctctcgag tcatcaatca tccggaacag gtgactgacg aattgaaaga
    14761 gttagttttc tcagcaatgg ctgaactgga ttaccgtcca aatattgctg caaaagcact
    14821 tgtcacgaat cggtcgcaga tcatcaaatt atttatactg gaagaaatcg acacaactga
    14881 gccctattac atgaatttgc tgatgggaat cgctaaacgg atcggtaaag cgcattattc
    14941 tctccaatta gttacaaatg atgccttcga cgtcggttct tgtgatggat acatcatcac
    15001 aggtgtcaga aaaaaagact tcgattggat cgaccgtttg gaaaaaccag ttgtcttgtt
    15061 tggggaaaat tctgaaggat atgattatgt agacagcgat aatgaatatg gtacagcaaa
    15121 agcaacgcaa tatgccttag aaagagatta tcaaacgatt atttatatcg gtatcgaagt
    15181 ggacgagcct tttgaaattt caagggaaac tggttattta tctgtgatga aggaagttga
    15241 tcttcctagt aaaatcttgc ggtttccaaa tcattcgaca gaagtagaaa gatatatgac
    15301 agaaaactgg gcagatttta cagataaaac ttgttttgta tgtagttcag atcggctagc
    15361 actaggagca gtaagaggga ttataaatgc tggaggacac ttgcccgaag aatttggtgt
    15421 gattggattt gatggggtat ttttagacca aatcagttca cctaaattga caacgatcaa
    15481 gcaagatatt atacgtatgg gtgaagtatg tggagaaatg ttgttaaaaa aaatagaaga
    15541 aaaaggggcg agtcaaggtt atcgtcgttt ttttcctgaa ctagttatca gagagacaac
    15601 gaattaagtc ttattactat atatatattt atgaaattca tcgctatttt cttaaaataa
    15661 tcagcgcatt gctactgaaa acaatgaatc tgtgctatta ttgagaaaag ttccattttt
    15721 gagaaggatg gtttgaatga aagaaatcag acgaaaacat ttccgttttc ctgcgtacga
    15781 tgatgagtta ggcgtaaaat taaccaacga caagaagcga gtattatttg acggacaaga
    15841 agatcttttg actgaacaga atcagtcccc gtttgaacag atgaaagaat ttcatttcac
    15901 gaacacgaac accgagaaaa tccgtcgcaa gcgtcgtgca acaaatggat tgaagtcagc
    15961 tccgcgtaag gaaaatttgg aaaagcataa aggaaaatta ccggattata ccggccatta
    16021 ccaatcagaa atcacatcga caggacgaaa aaaacttttt ggtgattcct ctcgttctgt
    16081 cagacaaagt gaaacaagga cagagtccaa gcatactaca cgaaacgcat ctgttgacac
    16141 gaaggtgcgg gagcgttctt attttgttcc taaatatgta cccgcatcga ttatcccaga
    16201 ccctagagaa agcaaaattt ctgaggatca tttaatcgaa tcgatgaaaa aaacggatga
    16261 ctcttatctg ttgttcgata cagaaccgac gccgtatcaa gaaaaaagaa atggtgagcc
    16321 gtctgtacga acattcaatc gaacaaaaac gttagaggaa acaaagcagt cagaagaaaa
    16381 aaaatcgaaa aaaagccatg gtatcttaga acgctcatta caaggattga tcgatgatca
    16441 aggaaactct ttgggagaaa atagctattt taaataagtg atatacaaat ttatattcgg
    16501 ctacagttgg aaaggaaacg ctgcggcgtt tccttatttt atactctatc cgaaagttta
    16561 tatcgtatcc tcaaattttt ttgagtttaa gcgaattttt tctcccaata agtaagaaaa
    16621 taatggcaaa ataaaaaaac aaactatttt cgcagatcgt ttgcaaaaat
//
LOCUS       DAMYXM010000055        16639 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.056, whole genome shotgun sequence.
ACCESSION   DAMYXM010000055 DAMYXM010000000
VERSION     DAMYXM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.056"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(45..410)
                     /locus_tag="PNS21_002035"
     CDS             complement(45..410)
                     /locus_tag="PNS21_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159737.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            618..1100
                     /locus_tag="PNS21_002036"
     CDS             618..1100
                     /locus_tag="PNS21_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159738.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1297..2187
                     /locus_tag="PNS21_002037"
     CDS             1297..2187
                     /locus_tag="PNS21_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HDI5159739.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2512..4983
                     /locus_tag="PNS21_002038"
     CDS             2512..4983
                     /locus_tag="PNS21_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDI5159740.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(5086..6822)
                     /locus_tag="PNS21_002039"
     CDS             complement(5086..6822)
                     /locus_tag="PNS21_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDI5159741.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6819..7523)
                     /locus_tag="PNS21_002040"
     CDS             complement(6819..7523)
                     /locus_tag="PNS21_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI5159742.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7654..8157
                     /locus_tag="PNS21_002041"
     CDS             7654..8157
                     /locus_tag="PNS21_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDI5159743.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8117..8455
                     /locus_tag="PNS21_002042"
     CDS             8117..8455
                     /locus_tag="PNS21_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDI5159744.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8476..9894
                     /gene="ffh"
                     /locus_tag="PNS21_002043"
     CDS             8476..9894
                     /gene="ffh"
                     /locus_tag="PNS21_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDI5159745.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10006..10584
                     /locus_tag="PNS21_002044"
     CDS             10006..10584
                     /locus_tag="PNS21_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDI5159746.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10588..11109
                     /locus_tag="PNS21_002045"
     CDS             10588..11109
                     /locus_tag="PNS21_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDI5159747.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11188..11742)
                     /locus_tag="PNS21_002046"
     CDS             complement(11188..11742)
                     /locus_tag="PNS21_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDI5159748.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11995..12270
                     /gene="rpsP"
                     /locus_tag="PNS21_002047"
     CDS             11995..12270
                     /gene="rpsP"
                     /locus_tag="PNS21_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDI5159749.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12282..12533
                     /locus_tag="PNS21_002048"
     CDS             12282..12533
                     /locus_tag="PNS21_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDI5159750.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12658..13449
                     /locus_tag="PNS21_002049"
     CDS             12658..13449
                     /locus_tag="PNS21_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159751.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13619..14140
                     /gene="rimM"
                     /locus_tag="PNS21_002050"
     CDS             13619..14140
                     /gene="rimM"
                     /locus_tag="PNS21_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDI5159752.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14130..14891
                     /gene="trmD"
                     /locus_tag="PNS21_002051"
     CDS             14130..14891
                     /gene="trmD"
                     /locus_tag="PNS21_002051"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDI5159753.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15027..15374
                     /gene="rplS"
                     /locus_tag="PNS21_002052"
     CDS             15027..15374
                     /gene="rplS"
                     /locus_tag="PNS21_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDI5159754.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            15632..15925
                     /locus_tag="PNS21_002053"
     CDS             15632..15925
                     /locus_tag="PNS21_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDI5159755.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         5585 a         2964 c         3379 g         4711 t
ORIGIN      
        1 aaaaagaaga aagtagcttc tctttgtaca tatatggttt gtttttatgg atgccgagct
       61 tttttataag ataaggctac cgcccataac gacccaagga tttgtggaac taagtaaata
      121 cctgcaagaa gcaagatcag ctgggtcggt tttaaaggat gatagaagta acgccattca
      181 gtaaaattgg cgcaaaataa ataggaagtg acagtagata taatattggc aatgaataaa
      241 gatataatac gcctgttcct ttgaaaataa tatccagcaa aaagagataa aaatatcaat
      301 gtgatgaacc cgcttacgga atgataacga aaatcaagca gtaaggcgaa atgcacataa
      361 ggaacaaaag ataaaagcaa cggaaggttt ttccagcaag tttttatcat ctttcatcac
      421 cacctaacaa gtgttattat tttgttagtt ttattgtaaa ttgaatcctg caataatcaa
      481 ttactttttc attatttcta gattaaatga ataaactctc atgccattat ctctaaaaaa
      541 aaagtttttc cccctaattt ttttagatag aaaattgtat agtatgatga gagaaaaagt
      601 agtttggggg aacgttaatg agaagaaaag cagtaatcga tcggctttat ttattttttt
      661 ggttttttcc tatcgttttg ctacctttta catgggtaaa tgctgttgta caaggtgcgg
      721 agatattagg cctgatccaa ggaagcggat ttgtggtttc taggaatttt ccagctttga
      781 ctgttttcta tttagtgata agtgtaggtc tcttattacc aaaatctctg atcaatcact
      841 acaattggca gctgctttac ttttcttgct tattagtatt tacatctatg ttttctttgc
      901 tgcctcgctt gattatcggc cctaatggtt tgagcgagag tggattgaag gattcagctt
      961 attcctccat gtatttcttg acgatcaaac catggttcta tttagtaatt gcctcattta
     1021 ttcttgcaac atttttttac ttctggacag aaatcaggat aaaaagtgaa cgatctgtat
     1081 ctataaaaaa taactattga tggtggatca tcagtaaaaa agcatatgag ctgcgtaaaa
     1141 tgcatgtttc tcatatgctt tttttattat tatgtaaaga atgcgaaaaa cgtttacatg
     1201 atttttacat aacttaacta tatttttaca gcaaccatac gagggattta cagtcgactg
     1261 ataaacttta gttgtaaata agaaaggagc aataaaatga aaaaagtatt gtttactcta
     1321 ggtatgatgg gaattctttt agccggatgc ggctcaggat caggaaatac agatggctct
     1381 gccacacaaa ataattcaag tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg
     1441 acagcattac agccgttagt cgatgcagca caagaaagct ttgtacaaga aaatccaaat
     1501 tatcaaatct ctgtacaagg aggaggaagc ggaactggtt tgagccaagt cgaagctgga
     1561 gcagttacta tcggaaactc tgatgtattt gcagaagaaa gagacggagt tgatgcttca
     1621 aaattagtgg atcataaagt agcagtggtc gggatggcgc caatcgtcaa taaagataca
     1681 gatgtcaaag atatcaccaa acaggaattg attgatatct ttacaggaaa aatcactaac
     1741 tggaaggaag ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc
     1801 ggtacccgcg cgacattcga aaaatgggga ctggatggtg ctacccctgt gcagtcccaa
     1861 gaacaagatt catcaggaac agttcggcag ctggtaagtc aaacaccagg agcaatcagc
     1921 tatttagctt tctcatatct cgatgattcc acacaagcac taagtatcga cggtgtagaa
     1981 ccaaaagaag agaatgtagc agataacagc tggggaatct ggtcatatga acatatgtac
     2041 acgaacggaa aaccttcccc tgaagtccaa aaattcttag actacatgat gacagaagaa
     2101 atccaagaag gacctgtcaa agaattgggc tatttaccaa tcacaatgat ggaagtagaa
     2161 cgtgatcacg aaggaaatat caaataacta gcagataaac atgccacggt agtcgttact
     2221 attaaggaaa aatcaactat gtttcccaca tatacatttt tcctttaatt gtgaaaaata
     2281 gttcctcata ttttgccgtt ttttgagcta ataccgaggg tcagctgatt aaacaccgtt
     2341 tattcgaaaa aaagagctac gacatagaac tgtcgtagct ctttttttgc tatatgacag
     2401 gaatgaaagg tgtttgcaag cttaatgtga ggaacacttt ccatatctgt tctatactgg
     2461 ataggagaag tttggaaata tccagaaaac gaaatcaatc gaggtagcca catgaagaat
     2521 aaatggataa atcgaagtaa aaaaaagaaa gtaacacaaa gatcatcaga taaagtcatc
     2581 cgtctttttc catcgtataa agaaggatta acggcagatc aagtcaagga acgaatagaa
     2641 aaaggtgcag caaataattc agtcgatccc acattcaaaa caaatcagca aatcgtattg
     2701 gaaaacatat ttacgtattt caatttgatc tttcttatct tagccatttt gctttgttta
     2761 gtagaatcct ataagaactt gactttttta ccagttatca tagccaatac aggaatcgcc
     2821 atttttcagg aaattcgatc caaaaagatc ttggacgaac taaatgtttt aaatacagca
     2881 aaagtcaatg tcatacggga cgggatagaa caaacaatag atatagaaga ccttgtaata
     2941 gatgacattg ttattttaga gactggacat caaattccag cagatgcaga ggtcgtggaa
     3001 ggaaaactgc aagttaatga ggctttattg acaggcgaag cagatgaaat aaccaaagaa
     3061 ataggcgatc cattaatgtc cggcagtttc attgtttccg gaaaagctta cgcccgatta
     3121 gacaaagttg gagcagattc gtatatttcc cagctgacag tcaaagccaa gacaatggga
     3181 gaaggagaac agtcggagat gattgcttct cttaatcaat tgatcaaatg gattgggatc
     3241 atcattattc ctatcggtat cctgcttttt tctcaaagtt acttttttaa tggaaacacg
     3301 atcaaagaaa gtgtcgttgc aatggaagca gcattgatcg gtatgatacc tgaaggactg
     3361 tacttgctga cgacgattgc attagcatta agtgcgacta aactagcaaa gcaaagagtt
     3421 cttttacaca atatgaaaag cattgagaca ttggcaaggg taaatgtcct ttgtgtagat
     3481 aaaacaggaa cgatcacaga aaataaaatg tctgttcaaa aagtgatcgt ctctaaaaaa
     3541 caagaaataa tcagtcagtc agatcaattg gaagcatgca ttgctgacta tgcaaaagcg
     3601 atggcaggag acaatgcaac aatggaagct gttaaagcat attttacaaa aaccactgat
     3661 caatcctatc accatgttat tcctttttct tctgtccaaa aattcagcag tgtagcgttg
     3721 gcagacaagg tgtatgtttt aggagcgccc gaaatggttt tgcgcgatca attgccggaa
     3781 tatgcggaag agtttgtttc tttcgctgaa aaaggctatc gcgtgttggt ctttggtgtg
     3841 tacgatggga ttcttgaaga accagtattg acagaagcag tcacaccatt aggctatatt
     3901 ctgattgcta atcctatcag aaaagaagca agagccacat ttgattactt caaaaaacag
     3961 caagtagcca tcaaagtgat ttctggagat aatccgctca ccgtatccaa tgtagctgtc
     4021 caagcaggga tttcacatgc tgatcagtac gttgatgcat ctaaattatc agaagaagaa
     4081 tatgaagcag ctgtcgaaaa atatacagtt tttggacgcg tcaagccaga acaaaagaaa
     4141 atcttcgttc aattgataaa gcgtaacaat acagtagcta tgacaggtga tggcgtgaat
     4201 gatattctag ccatgaaaga agcagactgc agtatagcga tggcttccgg aaatgaagcc
     4261 gccatgcagg cctcacaagt ggtcttactt gattctgact tttcacgcat gccagaagtt
     4321 gttgcagaag gacggcgcgt tgtgaacaat attgagcgat cagccagctt gtttttagtg
     4381 aagaatattt tctccttttt gctctcactg ttttcagtga tctttgctct gacctatcct
     4441 ttagaaccat cacaaatcac attgattagt ttgtttacta tcggacttcc atctttcttg
     4501 cttgctctag aagaaaacaa aaagcgaatc agagggaaat tcatcatgaa tgtgatggaa
     4561 aaagctgtgc caggtggact aacagatatg atcgtagtcg gtgcgttggt gatctgcggg
     4621 gtgactttga atctaaataa aacagatgtt tctacagcat ctaccatgct tttgattgca
     4681 gtagggttcc tcgttctcta taaaatctgt tcaccgctga ataaattcag aagtcagatc
     4741 attcttttct gtgctagcgg taactttttt tcggtcatct ttttgcataa gctgttttca
     4801 atcaccggta tctcagctgt atcacttttg ctgttgagca ttctattttt ctcggcagat
     4861 tcgattttta ggcatttgac cacattggta gaatatttgt ttgaacgtaa aaatgaaaaa
     4921 gagcaaaagc cgtttagtcg cgtagctaaa aaatttctat cgatatttaa acgagaaaat
     4981 tgacgaaaag tatctcaaaa aagagtaaaa aagaacaacc actcgtgtat ctttcgaagc
     5041 aggatgagtc atccaacttt aaaacgcacg agcagttgct cttttttaca ttttcggaat
     5101 ggtaacggtg aaagtcgaac ctgttccaag atgactgtct acagtaatcg taccattaag
     5161 gagttgcacg tagtctttta caatcgctaa ccctaatccg gtcccgcctg aatgtcggct
     5221 tcgcgccttg tctacgcggt aaaaacgctc aaagatccgt tcctgatcct caatgtcgat
     5281 acctatacca aagtcttgga cagaaaacga tagatcatct gttgcctgat agcggataat
     5341 aatctcgctg tcgctttttg aatattggac agcattttca atcagatttt tgataattgg
     5401 ataaaggata tctgttttag tagtaaatgg ttcgttcttt ccaatcaatc gaatcgtcaa
     5461 ccgtttcttt tcgatgattg gctgataatt ttgaacgatt tgctggaaat aaggttctgt
     5521 ataaattgtc tggatctcgt aagaattctc gccatctttt gacagctgga tgatttcccg
     5581 aatcagttta tctaaccgga tcgcatcttt ttgcatgatc tcaagaaaag aagtcagagt
     5641 ttgcgggtca tcttttgcac catccagtaa agtctcggta aaaccaatca gtgaagtcac
     5701 aggagttttt aattcgtgag agacgttgcc aacgaaatcc ttttgtagct tttctaaacg
     5761 tcgcactttc gttaagtcat acgccacccc tagaatctgt ccggtacctt ctgaattagt
     5821 aaagtagcgc aaactcaagt ctaatgtctg ccgaccttct gtaagggtga tctcctgatg
     5881 gatcagcgga tgctcaggag tcacttgatg aatcagctga atgagttttg gctcttgaat
     5941 gacttctgtg taccgctgtt caggttgata gcttaccacg tttaaatgta gctgcatctt
     6001 tggattaagc agtagtaaac gagaatcagc atcatcaatc aggaatacgc caatcatcag
     6061 ttcgttcagt aacgtatata attgctcttc tgtggtagta cacgcccgat acatcttact
     6121 catttgttcg ctcaacgcat taatcgtctg atacaattct tcccattgat cgggtgtttg
     6181 catgattact tccgcctgat taggattttg caccattttt tttagtacgg gcagcacggt
     6241 tttcaatggc tggttttttt gatagatcag ataatagatc attcctgcaa gaacaaggaa
     6301 gaaagacaga aagaagaaaa agacccaacg gcgaaaactc tcagttctcg gtaaaaaacc
     6361 gcttgtcggt tctgcgatac gtaaaatggc ttctaactgg ccactttttt tgactggtaa
     6421 tgcgacatat aaaagatcct cattcaaagt agtacttttg cgcaaagcgc tacctaggtt
     6481 cccaccattc aatacggctt tgatttcggg acgggagttt cgctgttcat tcaatgtttc
     6541 attgtttgtg tcaaataaga tctttccagt agcatccatt aaagtaatcc gttcgttcga
     6601 ctgatggacg aattcttcca aagcagtttt gttttgcgct gcttggaggt tttcgacatc
     6661 taattgccgg atcagcagtt gtgctttttt tgtcaggtag ctttcttgct gttccacgat
     6721 ctgctgttga aaataatggg aaatcaattg ccagccacca aaaaacaaac taactaaaag
     6781 aataagaaag aagagacgtt cataaatttt tttccatttc atcgtttggg ttcctgaaac
     6841 ttatagccaa atcctctaat agtcaccaag tactttgggt gttttggatc tcgctcgatt
     6901 ttttctcgca aatgactaac gtgtacatct acgattcggc tttgtcctgc gaagtcaaaa
     6961 ttccaaatgc ggtctagcaa tgtgtctcgg tcgatcacac ggtcttttcg tttcatgaaa
     7021 taaacaagta attcaaattc tttcggtgtt agttcaatca gatggtcgtc tacggtcact
     7081 tgataattgg tcaaatcagc agtgatttgt ccaatcgata gatattcagg ctcagcttca
     7141 tcttttgatt gcttacgagg ttcaattcgt ctgaatattg ctttcatacg ggctaatact
     7201 tcgcgaggac taaatggttt ggtcaaatag tcgtcagcac ctatttctag gccaatgatc
     7261 cgatccactt ggtcatcttt agccgtcagc atcaaaatcg gtgtttcatt tttttcttga
     7321 cgcagccgct gagtgatctc catgccatcc atcgttggaa gcatcacatc taaaatgatg
     7381 aaatcaaatg attgttctaa tgctaattct aatccttcgc ctccatctgt ggcactggtt
     7441 accttgtagc cttctttttc caaattgaac gtcaagagcg ttacaataga aggttcatca
     7501 tcaacaacta gtattttttt cattcaaatc tcctttaaat aggtttcatt ctgtttatat
     7561 tttagcatag aatcagtaca aagggctatt ttacaaaaga aataaatggt ataataggaa
     7621 ggcactttgc tgaatagaaa ggaagagcag tcaatgattg gaatcagtgc atgtttaggc
     7681 ggtctggcct gtcgctatga tggacagaaa aaagaagtac cagaattgat ggaactagtt
     7741 gaaactggag aagctgtcat ggtctgtcca gaagtgatcg gcggattacc aattcctaga
     7801 gatcccgctg aaatcattgg tggaaatgga ttcgatgtgt ggaaagatca agcaaaagta
     7861 tggacgacaa gcggcgaaga tgtaacggaa gcgtacaaaa atggcgcgat caaagcctat
     7921 caaaaattgc aggaaaaaca cattgatcta gtcatcatga aggaaaaaag cccttcatgc
     7981 ggaacccatt cgatttatga cggaagtttt tccggaataa aaaaaaatgg tgccggagtg
     8041 gctactgctt attttattca gcaaaaaatg actgtgctct ctgatgaaga atggctgaaa
     8101 actagaggtg agcagaatgg aaatcgaaaa gaccaatcga atgaacgcgc tctttgaatt
     8161 ttattcgacc ttattgacag aaaaacaaat gaactacatg gaattatatt atgcagatga
     8221 tttttcatta ggtgagatcg cggaagaata cgaagtaagc cgacaagcag tatatgacaa
     8281 tatcaagcgg acaagcaaga tcttggagga ttatgaaaaa aaactccatc ttttttctaa
     8341 ttatattgtt cgagaacagg tattggaaaa tatgactgca tatattacag ataagtaccc
     8401 ggaagataaa aaactacttg aatatgtaca gcaaattcaa gcaatcgaag aataaataaa
     8461 aggaatgaag aaaatatggc atttgaaagc ttaactgaac gtctccagca ggcgatggga
     8521 aaaataagaa aaaaaggaaa agtatcagaa gctgacgtaa aagaaatgat gcgggaaatc
     8581 cgccttgcct tactagaagc cgatgttaac ttgcaagtag tcaaagattt tactaagcgc
     8641 gtacgcgaac gtgcggtagg ggcagaagtg ctggaaagtt tatcacctgc acaacaaatc
     8701 gtaaaaatcg ttgatgaaga attgacgatc acacttggtt ctgaaacagc agagttaaat
     8761 aaatcaccaa aaataccaac tgttatcatg atggctggtc ttcaaggggc aggtaaaacg
     8821 acctttactg gtaaattagc caattattta aagaaaaatg aaaatgctcg tccgctccta
     8881 atcgcagggg acgtatatcg accagctgcg atcgaccaat tgaaagtatt aggtcaacag
     8941 ctagacgttc ctgtatttga tatgggaact gaagtcagcc cggtagagat cgtccgtcaa
     9001 gggatggagt tagcaaaaga aaagaaaaac gactatgtat tgatcgatac ggcaggacgt
     9061 ctgcatatcg atgaaacatt gatggatgag ttgaaacaaa tcaaagaatt gacgcaacca
     9121 aatgagattt tgcttgttgt cgatgccatg actggtcaag atgccgtcaa cgtagctgat
     9181 agcttcaacc agcaattagg tattacaggg gtcgtcatca ctaaacttga tggggatact
     9241 cgaggcgggg cagcactatc gatccgctca gtgacaggcg caccaatcaa attcatcggt
     9301 tctggtgaaa aattgactga cttagaagta ttccacccag atcgtatggc tagccgtatc
     9361 cttggtatgg gggatatgct gacattgatt gaaaaagcgc agcaagacta cgacgagaaa
     9421 aaagcggaag aacttgcaca gaaaatgcgg gaaaattcat tcgatttcaa tgactttatt
     9481 gaacagcttg atcaagtgat gggtatgggc ccactggaag atctaatcaa aatgatccca
     9541 ggaatgaatc aagtccctgg aatcgaaaac gtcaaggtag atccaaaaga tgttgaacgc
     9601 aaaaaagcga tggtttattc tatgacgcca gcagaaagag caaatcctga tctgttgaac
     9661 ccaagccgtc gtcgccgaat tgctgccggt tctggaaaca gtgttgttga agtgaatcga
     9721 atgatcaaac aattcaagga atcaagaaaa atgatgcagc aaatgtccaa aggggatatg
     9781 aacatcccag gtatggacca aatgtttggt tcaggcgtca aaggaaaact aggcaaaatg
     9841 gcgatgaatc gaatgatcaa gaaaaataag aagaaaaaga aaaaacggaa ataagatgaa
     9901 aaaaacagct tcggtacaga ctgaaaatgt ctactgccgg agcttttcta tcagcaattt
     9961 ttattcttta ctaatacaat tactctaaac gaagaggtgg cacagatgag atgtccgtgg
    10021 agtgaacaaa cagaaagtat gcggcaatat catgatactg tctggggtgt tcctgaatat
    10081 gatgaccaaa agctgttccg aaaattgatg cttgatatta atcaagccgg attgagctgg
    10141 cagacgattt taaataaaag tgaagcattc gatgaagcat atgatcgatt cgatatcaaa
    10201 aaagtcgcaa attatggaga aacaaaaatc agtgaactaa tggaaaacaa aggaattatt
    10261 cgtaaccgaa gaaaaatcga agcagccgtc caaaacgcca aagcagtctt gaaaattcaa
    10321 gaagaaatcg gcagtttttc agagtatctt tggtcgtttt ctaatggaaa aatcgttgat
    10381 tcttcttatc attcacaaga agaagtccca acaacaagtc ctctttcaga tgaattagca
    10441 aaagatctaa agaaaagagg atttaaattt atcggaagca caacaatcta tgctttttta
    10501 gaagctgtcg ggatcatcaa tgatcacctt gacagttgtt tccgcaaaga agaggtcgta
    10561 cgtatcggca gaaagggcga ataaagcatg cgaaaaatca tattttatgg tgctataagt
    10621 ttagatggct atttggcaac gaaaacggat gatcttcaat ggctttttga cactccaaca
    10681 ggtaagcaaa ctacttatga agctttttat gaaacaatcg atacgaccat catgggcaga
    10741 aaaacttatc aggaagcaaa aaaatatttg gagaccgaag caatttatcc agagaaaaag
    10801 aatttcgttt tttccaccca cgcacaatta caattgccag atgctgaggt cgtaaacgaa
    10861 gatcctgttg cttttctaaa aaatctacaa atgacagaag gaaaagatat ctgggttgtg
    10921 ggtggcggtc gattgatcaa accgctattg gaacatcaaa tgattgatga atggtatatc
    10981 cagattaccc ctgtattatt aggggacggg attcctttat tccaaaaagg aacatatgaa
    11041 gcgcgttttg aaatgctaga cacgacacga ttcggtgaat ttatcgaatt gcattatgta
    11101 cgaaagtaga agtaaataaa ataaaaaagg caactatatg cagaaatgtc ctgtacatag
    11161 ttgccttttt tattaccgtt atgcttttta ctctgttaat ttgttagaaa aatctagata
    11221 cgcatctaaa atcgtttgga agaaacttaa tgttccttca acgatattac cattttcatc
    11281 aattagattc gcaacgtttc cgatatatgc ttccggttgc tgcaaggttg gcacattcaa
    11341 aaagacaagt gattggcgta gatggtggtt tgcgccaaag ccgccgatcc cacctggtga
    11401 tgcagtaacg actaaacctg gtttatgatc ccaaacactg tgtccataag gtcttgagcc
    11461 tacatctaaa gcatttttca atactgctgg cacagaacgg ttgtattcag gagtaacgaa
    11521 aacgacaccg tctagtcccc tcacttcctc acgaaaacga gtccattcag caggagcctg
    11581 ttctggagtt tcgagatcct cattgtaaaa tggcagatca tcgattttta caaatacact
    11641 ttcgtaccct tcaggaagca atttcgcaaa agcttctgct acttttttgt tgtaagaatc
    11701 ttttcttaaa ctaccaataa aaaatccaat tttttttgtc atacgatttt tcctccttca
    11761 aactatctat aataatctta caaaagataa gtaactggag caaataaaaa cacttactga
    11821 caaaagctac atagaagata gattacttat ttttaacaaa aaagaagtct aaaatcttct
    11881 attttctaaa aaaaatcgaa tttaatcaag ctgtaaagaa aaaaagcttt acagggtaaa
    11941 aaaaaactgt tacactacat cttgtgaata aaacaatgga ggtggaaata aataatggca
    12001 gttaaaatcc gtttaaaacg tatgggttct aaaaagagtc cgttttaccg tatcgtcgta
    12061 gctgattcac gttctcctcg tgatggacgt ttcatcgaaa ctgtaggtac ttacaatcct
    12121 ttgaaagacc ctgcagaagt agttttaaaa gaagacttag ttcttgactg gttatcaaaa
    12181 ggtgcacaac cttctgacac tgtacgtaac atcctttcaa aagaaggcgt tatgcaaaaa
    12241 caccacgaag ctaaattctc aaagaaataa ggtgaccgga tatgaaagat ttaagcgact
    12301 tagtcttaac aatcgttcgt ccgctagtca cttatcctga tcaagtcaag ttagacgtcg
    12361 tagagtcgaa agacttttac gagtacaatc taacagtggc tcctgaagat attggccgta
    12421 tcattggaaa acaagggcgt gttgctaaag caatccgcac aatcgtttac ggtgttcgga
    12481 tcaatgcacc gaaaaaagtg cgtttgaata tcatcgatga taaggaaaag taaataaacg
    12541 tgctgtactc gaattttcga gtgcagcttt tttgtttttt ttattaattg aagaaatgat
    12601 tatttggcaa aacaagcaag aaagcgtatc cttataggag gagggagtga acagccaatg
    12661 agaaagaaaa tgttatttat tccatttgct ctattgttaa gcgcttgtag ttcggttact
    12721 caagaatcag acaatacaca tctatcaagc agtatatctg aaactgttac cagtccagaa
    12781 aaaagtagta ccaaaacggc ttccaccact gaacaaacaa tggctacctc aaataatgag
    12841 aaaaagactg ctttagacca gttgaaagaa caacagccaa atgttcccat gcctctagat
    12901 gtaccagtct cttcaggcta tctcaatatc gcagcgacac atacaaagca aggttattct
    12961 attttatatt acagaacaga tcgtcccctc gggttgaatg ctgacgaatt gaatcaagaa
    13021 acgcctattg caacctatct atatcaatat gggtttgctt ctagtcaaga aacaatccaa
    13081 gttttgcagc catttgagat cgacacaaat ggtcagcaag tggacttagg ttctcgaatc
    13141 acagggtatc agcaaggtgc agcaggttca agttttcttg aatggcaaga aggaaactgg
    13201 cgtattcgaa tccgtgggaa taatatagaa ggccaagatc ccttactatt agctaaagaa
    13261 atcgtagctt atttggaaga aaacagtcta ccagcacctg aacaatttgg taaaattact
    13321 gttgatatgg gtgatacgac gaatcgagcc gtagaagtaa gctggcaaga accgaaaaat
    13381 gcctatacga tcactcatca agatcctatg tctgcgttga agatggcagt ttctatgaag
    13441 aggttgtgaa atcagcgcgc tgatctgagc ccatcattta actccaagtc acaggaagta
    13501 atcaaaaata gcacgccgca tttttgcata attttggctt aataccttaa aagaaaacta
    13561 gaattgcctc ttcacacatg ctaaaataaa acagacaaaa aagaaatggg gatataagtt
    13621 gactgaatac ttgaatgtag gaaaaatcgt aaacacacaa ggtatcaaag gcgaagtccg
    13681 tgttatctca acaacggatt tcccagaaga acgttacaaa aaaggaacgg tgctaacact
    13741 attccaagac ggaaaagcac ctgtggaatt aacagtcaaa agtcatcgca aacataaaaa
    13801 ttttgactta ttgagttttg aaggacatcc ttctatcaat gatgtggaaa aatatcggga
    13861 tggcatatta aaagtgtcaa aagacaactc tgtagatcta gctgaaaatg aattctatta
    13921 ccatgagatc atcggtctgc atgtattaga agatggaact gagttaggta aagtaaaaga
    13981 aatcttgtca ccaggtgcca atgatgtttg ggtcgttcaa cgtccgaaaa aagctgatat
    14041 attgcttcca tatatcgctt ctgtcgtcaa agaagtcgat ttagaagctg gagtggtccg
    14101 tgtagaaatt ccagaaggac tgattgacga tgaagattga tgtattgact ttatttccaa
    14161 gaatgtttga aggtcctatg ggggaatcca tcatcggtaa ggctgtagat aaaggattat
    14221 tagatatcaa tatctctaac ttccgcgatt attcagataa caaacatcag acagtagatg
    14281 attatccata cggcggtgga gcaggtatgc tgctcaaagt ccagcccatc tatgataaca
    14341 tccaagccat tgaaaaagct gcaccggatg tcaaaaaaag agtaattctt cttgacccag
    14401 ctggtaaacg attcgatcag aaaatggcag aagaattttc acaagaagaa cacttagtat
    14461 tcatttgtgg tcattatgag ggatatgatg aacgtatccg cacattagtg acagatgaag
    14521 tttccttagg agattatgtc cttactgggg gagaattagg tgcgatggtc atgatcgatg
    14581 caacagttcg gcttttgcca gatgtgttag ggaatcagac ttcagctcaa acggattctt
    14641 attcaaccgg tttattggaa catccccaat atactcgccc ggcagaatat aaagggatga
    14701 aagtaccaga agtattgaca aatggaaatc ataaattgat tgaagaatgg caattaaaag
    14761 aatcactaag aaggacctat caacgtcggc ctgatctttt ggaaacactt gaatggacac
    14821 ctcaaatgac gaaattttta gaagaaatca agaaagaaga acaagaaaaa acagcagatg
    14881 aagccaagtg actttttcta aaatcttctt tacagtaggt tgataatatg ttagtatttt
    14941 atggtgagtg caaaagcact taactattac gatattccgc tgtgagaaaa gctcataaga
    15001 atatttggaa taaggagaag aaaaccatga atccattaat cgaagaatta acaaaagaac
    15061 aattacgttc tgacatccca gcgttccgcc ctggtgacac tgtgcgtgtt cacgcaaaag
    15121 ttgttgaagg tactcgtgaa cgtatccagt tatttgaagg tgttgttatc aaacgccgcg
    15181 gtgctggaat cagcgaaact tatacagtac gtaaagtttc taacggagtg ggcgttgaac
    15241 gtacattccc attgcataca ccacgtgttg caaaaatcga agtagttcgc tacggtaaag
    15301 tacgtcgtgc aaaattgtac tacctacgtg cattacacgg aaaagctgct cgtatcaaag
    15361 aaatccgtcg ttaatcattg ttatcagact gagaaagaac ccttggtatt caagggttct
    15421 tttttcttag catgtaattt gaacaccctg atccagtttt ttctttaatt attggtaggg
    15481 gagaataatg gattagaata aatctaccta taagtagtag taaaaaaaag agcactatga
    15541 taatatcttt cttaagagta accgctttaa taaaaaatcc agaatattct aacataaatg
    15601 gagtattctt agaatcaaaa ggagaaagaa gatggtttat acttatacgg agcaagaaaa
    15661 gatgaaagtt ttagacacat tcatgaatga tggaaaagta aaacaagtac caagttcaac
    15721 aaaaaagaaa tacatcctat tgaaagaagt cttgaaacgc tttgaccatg gagtgactta
    15781 tacagaaaca gaggttaatt cgatactgct gaacgtattt tctagtggcg attatgtcga
    15841 acaaagacgt tatctaatca catttggctt tttcaaaaga agttcagacg gtcgggcata
    15901 tcagatgatg ggaattgaaa attagaagtt ttaatttata ctcattgaaa aaagttcaag
    15961 tatgcgatat tggttctaca ccgcatactt gaactttttt tgtcatctgt atctataata
    16021 caaagccaag aacgttattc aaaatatgga tagctatgat aaaagctaat gagcgattcg
    16081 cagttaaata agcaatcgtc agtaaaatcg ttccaggagc gtacatgaaa aaatcaattg
    16141 gatgcgttac atgaataaag ataattctct tttatttgta cgaataaata ccaaaacgta
    16201 cctcctaaaa ttagattttg gacagatgcc ctttaaaaag ataacaagat ttcgttcttg
    16261 aaacaatata tatgctttta ttccaatgag gttgtgaaaa cagtattttg tcctgagcgt
    16321 tagaaaattg aaaatgggga atagcaactc gtactttacc attttttaag tgaatacaag
    16381 atcagctaat tgaacaccgt ttattcgtga tgagggacac aacactgcag tcgacacatt
    16441 tctcttttat gttcaaagat aacaaatatt taaaacaaag aaaaatagta gataatcccg
    16501 tcctaactct tttattcttc cctcatagct tccaaattta agcaatactt caattaatca
    16561 agttttatct ttttttttca ataatcgtaa aaaaagagta ttttttattt aaaaataaaa
    16621 tctttgtatt aaaggacaa
//
LOCUS       DAMYXM010000056        16639 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.057, whole genome shotgun sequence.
ACCESSION   DAMYXM010000056 DAMYXM010000000
VERSION     DAMYXM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.057"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(83..640)
                     /gene="frr"
                     /locus_tag="PNS21_002054"
     CDS             complement(83..640)
                     /gene="frr"
                     /locus_tag="PNS21_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDI5159756.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(643..1365)
                     /locus_tag="PNS21_002055"
     CDS             complement(643..1365)
                     /locus_tag="PNS21_002055"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDI5159757.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAHMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1501..2382)
                     /locus_tag="PNS21_002056"
     CDS             complement(1501..2382)
                     /locus_tag="PNS21_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDI5159758.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2481..3263)
                     /gene="rpsB"
                     /locus_tag="PNS21_002057"
     CDS             complement(2481..3263)
                     /gene="rpsB"
                     /locus_tag="PNS21_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDI5159759.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3622..4101
                     /locus_tag="PNS21_002058"
     CDS             3622..4101
                     /locus_tag="PNS21_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HDI5159760.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4318..5409
                     /gene="phnW"
                     /locus_tag="PNS21_002059"
     CDS             4318..5409
                     /gene="phnW"
                     /locus_tag="PNS21_002059"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDI5159761.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5402..6187
                     /locus_tag="PNS21_002060"
     CDS             5402..6187
                     /locus_tag="PNS21_002060"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDI5159762.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6533..8224)
                     /locus_tag="PNS21_002061"
     CDS             complement(6533..8224)
                     /locus_tag="PNS21_002061"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDI5159763.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8646..9593)
                     /gene="arcC"
                     /locus_tag="PNS21_002062"
     CDS             complement(8646..9593)
                     /gene="arcC"
                     /locus_tag="PNS21_002062"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDI5159764.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9708..10727)
                     /gene="argF"
                     /locus_tag="PNS21_002063"
     CDS             complement(9708..10727)
                     /gene="argF"
                     /locus_tag="PNS21_002063"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDI5159765.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10818..12047)
                     /gene="arcA"
                     /locus_tag="PNS21_002064"
     CDS             complement(10818..12047)
                     /gene="arcA"
                     /locus_tag="PNS21_002064"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDI5159766.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12508..13209
                     /locus_tag="PNS21_002065"
     CDS             12508..13209
                     /locus_tag="PNS21_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDI5159767.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(13781..14797)
                     /gene="tsaD"
                     /locus_tag="PNS21_002066"
     CDS             complement(13781..14797)
                     /gene="tsaD"
                     /locus_tag="PNS21_002066"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDI5159768.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(14794..15258)
                     /gene="rimI"
                     /locus_tag="PNS21_002067"
     CDS             complement(14794..15258)
                     /gene="rimI"
                     /locus_tag="PNS21_002067"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDI5159769.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(15265..15807)
                     /gene="rimI"
                     /locus_tag="PNS21_002068"
     CDS             complement(15265..15807)
                     /gene="rimI"
                     /locus_tag="PNS21_002068"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDI5159770.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(15791..16513)
                     /gene="tsaB"
                     /locus_tag="PNS21_002069"
     CDS             complement(15791..16513)
                     /gene="tsaB"
                     /locus_tag="PNS21_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDI5159771.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         4684 a         3451 c         2940 g         5564 t
ORIGIN      
        1 ctttttttaa tttttaaata aaagaaatat aaataaaaac agaggaagcg gaatgatccg
       61 cccctctgtt tttatgattt tattaaactt ccaataattc tttttcttta tcagcagcga
      121 tcttatctac ttctttaatg ctgttatctg tcaatgtttg tacgtctttt tctaatccgc
      181 gcaaatcatc ttcggtaata tcaccatttt tttgttgttt tttatattca tcgatcgcat
      241 cacgacggat gttacgaatc gcgatttttg cattttctgc ttcttttttc acatctttag
      301 ctaattcttt tctgcgttct tctgtcagtt gcgggataac taagcgaatg acatttccat
      361 cattggttgg actgattccg atatcacttg ccataatagc tttttcaaca tcttgaagaa
      421 tgctcttgtc aaaaggtgtg atcattaaaa cacgtgcttc tggaatttgg attgatgcca
      481 tttggttcaa tggtgttggc gcaccgtagt agttcacagt aatacggtcc aaaaggctag
      541 cgttcgcacg gcctgcgcgg atttgcccaa gttcacgttg aagattttgt gctgcttttt
      601 tcattttttc ttttgcttct gtcataattg catctgccat ttttatttcc cccttacagt
      661 tgttccgata ttttcgccta agattgcacg gcgaatattt ccgtgttcat ttaaattgaa
      721 gacaagcagt ggaatatcat tatccatgct tagtgaactt gcagttgaat ccatgacttg
      781 taatcctttg gcaataactt ccagatgagt cagttcttca aattttactg catttgcatc
      841 tactttcgga tcagcagaat aaacaccgtc tacattgttt ttagccatca ggataacatc
      901 tgcgccgatt tctgccgctc gtaatgctgc tgttgtgtct gtcgagaagt acgggttgcc
      961 ggtaccgcct gcaaagataa cgatccgtcc tttttctaaa tgacgttctg ctttacggcg
     1021 aatatatggc tctgcgattt gacgcatttc aatcgaagtt tgcacgcgtg tcggtacacc
     1081 cacgttttcc aacgtatcct gtaaagctaa ggcattcata actgtagcta acatacccat
     1141 gtaatcagct tgagcacgtt ccatgcccat atgtgctccg atttgtccgc gccagatatt
     1201 tccgccaccc acaacgattg ccatttcaac gcccagctcg tgaacttctt tgatttcttg
     1261 gatgatttct ttgattactg gtggtttgat tccaaatcct gcatccccag ctaaagcttc
     1321 tccactcaat tttaagacta cacgctgata tttcggtgtg accatgttca ttcctccaat
     1381 attttctatt gccattttac catacttaga tgatttcgac actctaagac tggtttttct
     1441 tgttttgctt aaaaaaaggg agcgcacaaa ccgtgcgttc ccgtctgtta ttcaacagaa
     1501 ttattttttc acttggctca taacttcttc tacaaagtta tcttcgcgtt tttcgatacc
     1561 ttcgccaact tcgaaacgta cgaatgtttt aacagttgcg ccttttgatg caacgtattt
     1621 ttcaacagtc atatcaggat ctttaacgaa tggttggtcc accaaagcga tttctgcttt
     1681 gaatttcttc aagcgtcctt ctaccatttt ttcaacgata ttcgctggtt tgccttcgtt
     1741 caatgcttgt tctgtaagaa cagttttttc atgttctaat tcagcttctg gaatttgtga
     1801 ttcgtttaca taacgaggat tgatagctgc tacgtgcata gctacgtctc ttgcaactgt
     1861 ttcgtcagtt gttccatcta gaacagtcaa tacggcaata cgtccgccca tatgcaagta
     1921 tccaccaaat gcagcattgt cgtctttttc tacaacttca aagcgacgga agctgatttt
     1981 ttctccgata acttgagttg cttcgatcaa gtcagattca attgtacctt tatctgtttt
     2041 gattttcatt gcagcttcca tgtctgctgg tttgttttca gcaacaagtt ctgcaatttc
     2101 ttttactaaa tcttggaaca tttcgttttt agaaacaaag tctgtttctg agtttacttc
     2161 aacgattgct gcagtgttac ctttcacagc aactgaagcc aatccttcag ctgcaatgcg
     2221 gtcatttttc ttcgcagcct ttgccattcc tttttcacgt aaaagatcaa ctgctttttc
     2281 catgtcgcct tctacttcaa ctaatgcttt tttcgcatcc atcattccta cgccagtcat
     2341 ttcgcgtagt tcttttacca ttttagctgt aacgtctgcc atgtttaact cctcctgtgt
     2401 gtttttcttt ttaaaaaaag ctgcttcaaa ggagagtgag gcttgtcgcc cagcctttga
     2461 aacagctcca tcattcaaaa ttattctgct gaagaattgt cgccttcaac aacatctacg
     2521 atttcttcga ttgatgttgc tgtgttttct tctgtgaaat cttcttcaac tacttgatct
     2581 tcaccttggt ttccttcgat gaaagcatca gccatttttg aagtaatcaa tttaacggca
     2641 cgaattgcat cgtcgtttga agggattact acgtcgattt catctggatc gcagtttgta
     2701 tcaaccatag ctacgattgg gatatttaat ttgtgtgctt cttgaacagc aatacgttct
     2761 ttacgtgggt caacgatgta cattacatct gggattcttg gcatatcagc gataccgcct
     2821 aagaattttt caagacgttc acgttctttg ttcaaaccag caacttcttt tttaggtaga
     2881 acttcaaaag ttccatcttc ttccattgca ttgatttgtt tcaaacggct gatacgtttt
     2941 tggatagtat cccagtttgt caatgttccg cctaaccaac gatggttaac gaagtattgt
     3001 cctgcacgag ttgcttcttc tttgattgct tcttgtgctt gtttttttgt acctacgaat
     3061 aatgctacgc cgccttcttc agcaacgctc ttcatgtaat cataagctgc atctactaat
     3121 ttaactgttt tttgcaagtc aatgatgtag attccgtttc tttctgtgaa gatatatttc
     3181 ttcatttttg gattccagcg acgagtttgg tgaccaaagt gtacgccggc ttctagtaat
     3241 tgtttcatag aaattactgc catgtttgtt tcctccgatt tggttttatt tttcctcttc
     3301 ttatcttcta ccttgccggc aaactttttc aagcacctac cgacaatcca ataagaatgt
     3361 ggatttgctg atgcataaag caccctcagc tattatacag acaaaagatc cgtatcgcaa
     3421 gagcaaatat acttttctgc tcaacattca atagtttacc tgaaagataa aaagaattca
     3481 agaaaaacag tagatttctc tttgtttctt tattatcctt tttccataaa ataattttag
     3541 gtataaaaga cgaatattat tgggcaatcc ttcttttttt tgttatgata catgtgaaat
     3601 ctttcgtaaa ggagaaagaa aatgcgcaaa gctcaaagac atttgctgat taaacaagtc
     3661 attgaagaat tcccgatccg tacgcaagaa gaactattaa acaaacttgg gcattatggc
     3721 gttaccgcca cgcaggcaac gatttctcga gatatccgtg atctaaaaat cgtgaaggca
     3781 cctgatgaaa atggcgtttc ccgcttcgta ttgtttcgcg aaaaaggaac tcctgagact
     3841 aggactgagg aagaggatcg gctggttcaa atgattgaag acattgtatt gaaagtcgaa
     3901 cgagtccact ttttgacgat cgtcaatacc ttgccagaca atgctcattt atttgcatct
     3961 gttttagatg agatcaaacc tccctctatt gtcagtacaa ttgctggatt cgatacgatc
     4021 atggtcatct cggaaaatga ggaggacgca aaacgagtag aagaatttct tcaccatcca
     4081 actgaagtat cactttttta atcagagcac gtattcctaa tatggaatgc gtgttttttt
     4141 attttcaata ttttccttat taaataaacc tttacactct ttacaaaaat acatcatttt
     4201 ttattcaatt ttttcaacac cttgattgaa tcacaataat catcatttat attggatgtt
     4261 aacaagagca gcttcttgct ctttgtgtat ttcaaaaaag aaatgaaagg aaatcatatg
     4321 tataaattat taactcctgg accattgacg acttctgaaa cagtgaaaaa tgctatgctg
     4381 atcgatcatt gtacttggga tcaagaatac aaagaaatta cacaatggat aagaaaaaaa
     4441 ttattggatc ttgctcaagt atcggaagga atatacacaa cggtcttaat gcaaggtagc
     4501 ggctcctttg gggtagaatc ggtattaagt tctgtgatca ggccagaaga tactgtactg
     4561 atttgttcaa acggcgcata tggacaacga atgatcaaca tgtgcgaatg tctgaaactg
     4621 aactataccg tttatgaggt atcggaacat caaatcctcc aagctcatgt gattacacag
     4681 ctgattgaag agaatccctc tatctccgca ctagcaatgg tccatagcga gactacttcc
     4741 ggtattttga ataatttgga agagattgct gctgtcacac aagaaaaaga tcttttgttt
     4801 atagtggacg cgatgtcaag ctttggtggg ataccgattc gtgtagccga actagggatt
     4861 gattttttga tttccagctc aaacaaatgt attcaaggtg ttccaggctt ttcttttgta
     4921 cttgctaata aagcggaact agaaaagcga aaaaatgtga gtcgaagttt gtcacttgat
     4981 ctatatgacc agtaccgcgt gatggaaaac gagaatggca aatggcgttt cacttcccct
     5041 acccatacgg ttttggcttt tcaacaagcg ctgaaggaac tagaggcaga aggaggaatc
     5101 gaaactcgat attcacgtta tctcgccaat aatcatgtac tacgacaaaa aatgacagag
     5161 atggggttcc agcctttcat tcaagaatat caggggccat tcatcacctc ttttctttac
     5221 cctaaggact ggcgatttga ttttaatcat ttttatcagt atttaaagca aaacggattt
     5281 gtgatctatc ccgggaaaat cagcgatcat gaatgttttc gtatcggaaa tattggcgag
     5341 atctatccgg aagatatttt ggaactgaca gcaattatca aagactataa ggagaacgtt
     5401 aatgattaaa actgtaattt ttgattgggc aggaacaact gtggactttg gctgtatggc
     5461 tcctgtccac gcttttagaa atgcttttct agaaaaagga atccaactga ctgacaaaga
     5521 aatccgcgaa ccgatgggaa aattaaaatg ggatcatatc caacagcttc tttccctccc
     5581 ttctgtcaaa aaacaatgga tagctattta tggacaacta cctcaggaaa aagaggtgga
     5641 ggaattgtat caacaatttg aacattatct gtttaaagat ttggtcaagc attctacctt
     5701 gaaacccgac acattggaaa cgatcgaacg gttgagaaac gctgggatca atattggttc
     5761 cacgactgga tttactgctc ctatgatgac aatcgtagca gaaaccgcaa aaaaagaagg
     5821 gtattcacca gacttcatgg caacttctga agacgtcgaa gggtatggtc gaccgtttcc
     5881 ttatatgatc cataaaaaca tgcagtactt caaaaacaag tcggttgatg aagtaatcaa
     5941 agtcggagat acggtctcag atatccagga agggaaaaat gccggagtgt tgacagtagg
     6001 tgtaattgaa ggtagttcgc taatggggat ttcttatgat gagtaccaaa aattagagat
     6061 ggggaaaaaa gatcgtttaa aacagtctgt tgctcttaga ttccaacaag ctggcgctga
     6121 tatttgtgta aacaatttaa aagagttaac agaatacatt ttgtacgctg acaagaatcc
     6181 agaatgactt ttatacaaaa tcagaggtcg tgaaaaagct gtactgcatc aagtaataag
     6241 aacagccaaa caaaaatagc tcctcatatt tttcgtttgg ttaggcttat taccgcagat
     6301 gcaagctttt gaacaccgtt tattcgaaaa aaaaagggag ctgtgacacg acttttgtca
     6361 cagtcccctt atttacaaac aaacagtgtt taagagacaa cctctccact aaacggtgtt
     6421 cattcagctg actctcggta tttgctcaaa aaacgataaa atttgaaaag ctattttctc
     6481 ttttgtttct ctaatactcg gatcagaacg ctgatttcac attctctttt tcttacatct
     6541 tatctggtgc atgaagtcct agcaaacgaa ggtcttcttt caataataca gtcactgcat
     6601 aaactagagc taagcgtgct tctttttgtt catcatcagc taaaatcttc gtatgagcat
     6661 agtatttgtt gaatgcttgt gctaatttga ttgcatgttt cgcaatcact gacggttcgt
     6721 atttttcccc agcagacaag accgtttcag ggtatttttg aactaattta accacttccc
     6781 agctggtatc gtcattcaat gcatagtctg ctgtttctga agggacaaat cctgcttttt
     6841 ccaacaggct gactgcacga gcatgtgtat attgcacata aggtccagtt tctccttcaa
     6901 atcgaacaac ttcttccagg ttaaagtcaa atgtattcag tcgatcgttt ttcaaatcgt
     6961 ggaaaatgac tgccccaacc ccaacttgtt tagctacagc atctttgttt tccaaatcag
     7021 ggtttttctc gctgatttgt tctttagcag attggattgc ttcatttaat acttcttcta
     7081 ataagacgat ctttccttta cgagttgata attttttacc accttgcgtg atcaaaccaa
     7141 atggaatatg gcgcatatcg tcagaccagt caaagcccat ttccttcaag acagctttca
     7201 actgtttaaa atgatagctt tgttcattcc ctactacata tagagattgt ttgaagtcgt
     7261 aagtacgttt acggtacaat gcagcagcta agtcacgtgt aatgtatagt gtcgcaccgt
     7321 ctgacttctt aatcaacgct ggattcaggt cataagcaga taggtcaacg atctctgcac
     7381 ctttatcctc gttcagcaaa tgtttttctt ctaaaagttt gacgatttca tccatcttgt
     7441 cattgtagaa agcttcaccg ttcaacgaat cgaaacgtac ttccagcaga tcatagattt
     7501 tattgaattc tttcatcgat tcatcacgga accattgcca taattgaata gcctcttcat
     7561 cgccttcttc cagacgtttg aaccaagcac gagcttcttc atttaattct ggttctgttt
     7621 cagctacttc atggaattgg acatataaac gaagcaattc attgattggt tctgctttaa
     7681 cagcttcttc tgtaccccat tttttgtaag cgacgatcag ttttccaaac tgtgttcccc
     7741 agtcacctaa atggttgatg cggattggct gataaccgat tttttccata atgaaaccga
     7801 tcgaattacc gataactgtc gaacgcaaat gtcccattga gattggtttg gcaatattag
     7861 gagaagacat gtcgattggt actgttcctt gattcccgat attgctatcc ccataatgtt
     7921 ctttttcttt cactactgtt tgcaaaactt ttttgctgat aagttcttta ttcatgaaaa
     7981 agttcaagta aggtccgaca acttcgattt tttcaaagtt ggcagaatcg attttttctg
     8041 ctaaatctgc agcgatttgc tgtggcgctt ttcggtaaac ttttgctaat gaaaaggctg
     8101 gaaatgcaac atctccgtgt tcagctgatt ttggattttc tagtaattgt tcgacttgtt
     8161 ccagtgttaa atcatctttg acagcgtcat agacagcttt tgcgacaaga tctttattat
     8221 tcatctttat tcctcctaaa gtgctccctt gattgttaaa caaaaaaaat ctctagcgac
     8281 ggcatactta gtatgccatc actagagacg agatttcccg cggtaccact ccaattgtcc
     8341 taaaaaagga ccagctccat ctgatatcgg tagacgaccg tctgtttaaa acagaattct
     8401 ccaagtgcgc ttcgttgtat cttcttcatt cggcttccac cacttccgaa ttcgctagcc
     8461 aagaatgata caagtactct cttgttcaac aaatcttagt cttattaaac tttcttgaat
     8521 tatatcagaa aaaaaaacga aaggctagtc gtttctgata aaaaaacaat ccagatggcc
     8581 taaaggagaa gaaataccac ctggatagct ataaatcatt tcgtaaatac gaaagactta
     8641 acaaattaat cagctacgat aatcgttcct tcttcagaag ctaaaagatt ctcgatattt
     8701 tctaaagaag tgatgattgc cttagcgttt ggttttgctt caacaaatgc aatagctgcc
     8761 tctactttcg gaagcatgct tcctggagca aattgctttt catcaatgta ttgtttcatt
     8821 tctgaaacag taacagtttc taatttcttt tgatctggtt tttggtaatt gacatacaca
     8881 ttgtctactc ctgtcaatac gatcagcaaa tccgcatcga tgatttcggc taatttttct
     8941 gaagcaaaat ctttatcgat tacggcttct cgtccttcta atccagtagc tgtttctaca
     9001 acaggaatcc caccgccacc aacagagact gtgataactc cttgatcgac cagcgtttcg
     9061 atgacacgtg cttctttaat cgagatgggt ttaggggaag cgactacttt tctccatcca
     9121 cgtccagcat cttctttgaa tgtcacagtg gagtcagcat tcatttgttc tttggcttct
     9181 tcttccgtgt agaatggacc gactggttta cttggatttt tgaatgaagg atcattttca
     9241 tccacaataa cttgagtgac taaagaaacc acatctttat cgatcccttt ttccttcagt
     9301 acttcgccca tggcattttg taaccagtaa ccgatagatc cttctgtcat tgcaacacat
     9361 gtatctaaag gcattgccgg tgttttctca gaatcagcag cttgctgctg aatcaatagg
     9421 ttaccgactt gcggaccatt tccgtgggag atgatcaatt catctccttg ttcgataaat
     9481 ttcactaaat actttgctgt ttccattaac gcttcttgtt gggcttttgc acttgcatcc
     9541 gtagacaaga ttgcattacc ccctaaagcg acaactactt ttcgtttggc catttcattc
     9601 gtcctttctt cagttctacc gaattttcta gataacaaaa cgagccagcg ttttgtgatt
     9661 ttccctcaat gctgactcgt tttttcgtga gatgaatgtg gttatgctta aacgcgaggg
     9721 ataaacaagt tacctaatgt tgctgccatg atcgctttga ttgagtgcat acgattttct
     9781 gcttggtcaa attgacgagc atatttgctg cggaagactt catctgttac ttccatttca
     9841 gtgatgccaa agcgttcttt catttgttca ccgtaaacag tgtttgtatc atggaaagct
     9901 ggcaagcagt gcaagaagat caaacgatct gtgttgtgtg ttttttctac catttccata
     9961 ttgatttggt aaggtttcaa caatttgata cgttcttcga atttatcttc ttcacccatt
    10021 gatacccata catcagaata aaggacgtct gctccgtcta cacctttatc aacatcgtcg
    10081 gtaatcatca attgtgcacc ggatttttca gcaaagcctt ctgccatttt gacgatttct
    10141 tcttcaggtt gtaattcttt tggtgcaacg atacgcatat tcgctcctaa aattgcacct
    10201 gtgactaata aagagttagc catattgttt cgaccatctc cgcagtaagc aacagtgata
    10261 ccttctactg ttccaaagtt ttcttggata gttaaaaagt cagcgatcat ttgtgttgga
    10321 tgccattcat cagtcaagcc attccaaact ggaacaccag agaattctgc tagttcctct
    10381 accatttttt gactgaatcc acggaattcg attccatcaa acatacgacc tagtacttta
    10441 gctgtatctt ctgtggattc ttttttacct aattggatat catttgcacc tagatattca
    10501 ggatgtgcac ctagatcgat cgctgcagta gtaaatgcag aacgagtacg tgtagatgtt
    10561 ttttcaaata aaagcgcaat attttttcct tctagatagt gatgtgggat accacgtttt
    10621 ttcaagtctt tcaagtgttc tgaaaaatcg atcaaatatt gtaattcttc ttttgtaaag
    10681 tctttttctg ctaataagct tcttccttgg aatactgatt ctttcatatt aaacattctc
    10741 ctttagattg tttttattta agattaaaga ggaaatcccg acggcagctg acagtaagac
    10801 cgccgtcagg aaacaaatta cttcaagtct tcacggacaa gtggttggct catgcagcgt
    10861 ggaccaccac ggcctcgtga caattcactt gaattgattt ctaacacttt gatgccatag
    10921 ctacgaagca attcgttcga tacataattt cggttataag tgactacaac acctggagcg
    10981 attgccaatg tgtttgatcc atcattccat tgttctcgag gtgcaacgat ctcatctcca
    11041 ttaccagttg ggatcagtac aagatcatct aatccaagag cagctttcat cgtttcgtgt
    11101 aagttatctg aatgagtaat cttgatatca tcaccatctg gttcgattgt aaagacatcc
    11161 atcttaccgt ctttactttg aattgctgga tggatagtga atttatcgtg atctaccatt
    11221 gtaaatacgg tatccaaatg catcattgca cggttgtttg gaattttgat tgctaacact
    11281 ttttcaaatc ctgaattttt tgcgaatagg tggcgtgcta atttttctaa agctttcgca
    11341 ttagttcgtt gagaaatccc aacagctact actttatcgc ttaagatcaa ttcgtctccg
    11401 ccttcgatat gatctggatt ttcacgatcc aaccatactt cgattccttt atttgcaaat
    11461 cttggatgat gtttcaaaat atattctgta aacatcgatt cacgacgacg tgcttcaaaa
    11521 gtcatcttat tgactgtcat cccatttccc attgatgcgg aagggtcgcg tgtaaagtac
    11581 aagtttggca ttgggtccat gtaaaatgga tagtcatcgt ctgctgacag atcgtataga
    11641 ctgctagaac gtacttcaat atcttttgta cgaaccccag ccatgatttt atctaccatt
    11701 tcttgcgttt ccattgatag aaggaactca tgaagaccct ctcttacagc attcgatgca
    11761 atatgagatt catccaacat tttattcaag aattgttctt tgacattgcc tgcgtcaatc
    11821 gcttctgccg ctaatttttc caagtaaaga gtctcgacgc cttcattttg caatgtcttt
    11881 gcaaagttgt catgttcttc ttgagcaatc ggaagatatg gaatatcatc gaacaacaga
    11941 cgatccataa tatctggtgt caaattttcc acttcttgtc caggcctttt cagtaaaact
    12001 gtctttaatt tccctatttc agagaaaacg tgaataggtt tatccatgtt gtgttgcctc
    12061 cttctttttg attacatgta taggatatcc tccttttgta atcactttca taattttgtc
    12121 tcattcaaaa tgagacattc ctcacattaa tattcgataa ttaaataatt aaacgttttt
    12181 ctatttttaa gcgtaattta ttcatttttt attctgtata cactttcaaa aaaatattta
    12241 ttcattgaaa aaaatttttt gaataaatta aaaaaataaa ttatattagc gttgttagcg
    12301 tttactatat gtattattgt tgttttttat acacaaatca ttcgcttcaa aacaaattat
    12361 tcaataccat tgtatatttt ttacataata acaaattata tttgtaaagt attaaaactt
    12421 gtttctaatg ttggtatgtt aaaactttac aaattcttta aaaaaagtga caataaatat
    12481 agtgaacaat aaggagagga ctttttaatg cgtgttaata aaatcgattt tcacttttca
    12541 aaactcagat atcaacctga ttttgaacat ttctcggatg aagagttcca acagatcaag
    12601 gaacatactg ttcttcgttc ttataaaaaa ggacagatcc ttttcgacga tggtgatccc
    12661 agacaacggt tgtatatctt gaccaaagga ctcgttcgtt tagaacgtta tgatgaaagt
    12721 gcaacttatt actactatga ctatgtgaat acaaataacc tttttcctat gtcaggaatg
    12781 tttggagaag agacctacac ttatacagct caagcgatga ctgatattga aacattctac
    12841 cttcctgttt cctactatga aaaattagca cgaaaaaatc ccgaacagtt gatctatatg
    12901 atacaaaaaa tttctaaagt agttgaaata catgaattgc gtatccaaat cggattgacc
    12961 tctagcgcct ttgaccgggt aaaacaaaat ttattcattt tgaaagaaga attgggcgta
    13021 ccaacaaaag atggtcatat caccattcct tatccgatca ccttaaaaga attagcagtg
    13081 aatagcggca cgacacgtga aactgctgga caagtaatca aacaactacg tgattccgga
    13141 ctgttagatt atgacaaaaa gcgttttact ttctgtaaga cagctactac tccagctatg
    13201 agagaataac gcgaacaaaa ataagcagtg accctgatat ttatcagggt cactgcttat
    13261 tttcctccct aaaacttctg aaagatccgg cagttttaga tcactgggca agactcaaag
    13321 attttctatg tgtgtttcag ttatgtaata ttcactgttt gcaaaaaata ctgatagatt
    13381 aactagagga agcgtcttgt cctcatccga cctagattat tcgatattcc tcgacaataa
    13441 acgattaatc agtgagggca atcagaggaa cggataatga tgaataagct gtaaaaaata
    13501 gggctttgac aaaagcgtgt cacagccctt atttcgatta aacggtgtgt acctgtcaac
    13561 tcttcggtca caagtcataa tgttcataaa catgaggagc tgttttttga aattgttcct
    13621 tgtgaatcag agttaaacga caggcttacg aactcttgat taaacggcgt tcaaaagctt
    13681 gcatacgcgg taataagaac agccaggtca aaaacatgag aagccgtttt tcctgggctg
    13741 ttcttattac ttgatgcagg gcagattttt cacaacctct ttattcagat aaaaccaagc
    13801 ttggatcagc attcaactga tagctagcaa aatttctttt ttgcatttcc acatgtgctg
    13861 ctgctccaat cattgcagca ttatctccac aaagtcgaag agggggaatc actagttcta
    13921 cctcagggag tttagttgcc aaagcatcct gcaatccttc acgcaaccct tgattggccg
    13981 ctacacctcc agctacaatc agttgcttga ctggataatt ttgacaagct cgaagtgttt
    14041 tagtaatcaa tacatcgatc acacttgctt gaaagctagc tgctaaatca tttttgtcta
    14101 gatcttctcc acgctgttgt gcattatgca ccaaattgat aaatgcactt tttaatccgc
    14161 taaaactgaa atcataatta tcttcatgga tcatcgctcg tggaaaatgg taattatctt
    14221 ttccctgatg agctagctga tcgatttctt ttccgcttgg gtaagaaaga cctaatactc
    14281 ttccaacttt gtcataagct tctcctgctg cgtcatctct tgtctcccca atgatttcat
    14341 atgatccatc ttcttgcata tagactaatt ctgtatgtcc tccgctaaca agaagtgcca
    14401 tcaatggaaa ctggaatggt ttaaccagac gggctgcata gatatgccct gccatatggt
    14461 ttacaggaat aagcggcaat tggtgtgccc aagcaaaagc cttagctgca gaaataccaa
    14521 tcagcaacga gccgacaagt cctggtccat aagtgacagc gactgccgat aaatcttctg
    14581 ccgacacacc agcttctacc agtgcatctt ccaagcataa ggtgatttgt tctacgtgat
    14641 ggcggctagc tacttcagga actacgccac caaaacgttt atgactgttt atttgagaag
    14701 caacgatgtt agataagatc tctgttccat ttctaacgac cgctacactc gtttcatcac
    14761 aacttgattc aatcgccaga atcaattctt cattcatttc atcgtctccg tttttaactt
    14821 tctgctcata atgatcgcat cttccgtcgg attgcgataa tatgatttcc gtttacctaa
    14881 tatacgaaac ttttctgatt cataaagttt tcttgctgct tcattagata gacgtacttc
    14941 taaaaaaaca tgcagcgaat cttttatttg ttccgtaatc agtaattgta acaaatgccg
    15001 tgcatgccct tttccttgtt ctgaaactgc aacagcaata tttgtgatct cgacttcatc
    15061 tattactttc gagtatccaa taaagccaca gatctggtta tcttcaacta aaagcagata
    15121 atcagagtga ggctgttgga tgtctgccaa aaactgcatt ttcgtccaag gtgaaccatg
    15181 tggataagca ttttcgctaa tcatccatac gcattctgcc aagtacgctt catcaaaatc
    15241 tttttttctg caaatcaact aatctcactc attttctaaa agttttatca tatccaacgc
    15301 atcttctttt gtttcagtgt aatatctttt tttgatcgtt gttgcttgaa aacctaactt
    15361 ccgataaagt ctttgtgcat cttgattact catacgcact tctaatgaca tcgtctcaca
    15421 gcgattcatt atagcaaagt tctcgatttc gtcaataagc agcgaaccga ttcgtttacg
    15481 ctgatatgcc ggtcgaacgg caatattcgt gatatggcaa tctaaaccga acgctcttga
    15541 accgataaaa ccaatcattc ttccattatc aaagatacct agatatagat gaggaagcgg
    15601 tgacttcaat tctgatataa aggcactttt tgtccatggc aattcgccat tatagacttc
    15661 tcgttcgaca tccagcagct ctttgatatc ttctgtagtc aattctctta gtacgtaacg
    15721 acgatcgatt tgttcgattg ttttttctgt ataaatcatt ttatcttgaa aaaagcttct
    15781 ggcatacgct ctaaattttt tcaacataac tttcgtctcc aggtgtgtgc gtctccaacc
    15841 atttttcttc tgcttctacc cgttttaaat agcgaggaag aaaagcttgt ttctctgcaa
    15901 ctggttcagc taaagcacct aattgagcca cagttacacc acttggaata tcccatgctg
    15961 gaactgtatt aatcatcgca ctaggcaata tttcctttat ttcatagaag aatttgtgac
    16021 aatcactccc gacgaaataa atagcttcat tcagcttttg tagttgtgtg aacaaatcag
    16081 aagcagaaat atgtacatct tcaagtacaa tttctaatct attttctttc cagcggtatg
    16141 ccccagcgta tacatttttt cttcgagcat caaacatggg cacaataatt ttatccacac
    16201 cgatgcaatt agccgcgatg gttttcagac tagatactcc tactagctct ttatctaatg
    16261 tataagcaat cgtttttgct gtcgttacac ctatacgaag ccctgtataa gaaccgggac
    16321 catcagaaac cgcgattcgg tcaatgtctt ttggacttat ttgaagatct gcaaacaatt
    16381 ggtcgattgc aggcatcagt gttacactgt ggttgcgttt gatatttgtt tgtatttgtc
    16441 caagaatttg ttcgtcttct accacaccga tcgccaaagt ttggtttgca gtatctattc
    16501 ctaatgtaat catatatttg atcctttctg ctatgacatt ctcgccttcc attgtagcat
    16561 acttttttat tctgaggatg aaaataccaa aggcaaaact agtacaatga tacatgatta
    16621 tctgctggcg ggcaaattt
//
LOCUS       DAMYXM010000057        16514 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.058, whole genome shotgun sequence.
ACCESSION   DAMYXM010000057 DAMYXM010000000
VERSION     DAMYXM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.058"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..546
                     /locus_tag="PNS21_002070"
     CDS             <1..546
                     /locus_tag="PNS21_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDI5159772.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
     gene            735..1403
                     /locus_tag="PNS21_002071"
     CDS             735..1403
                     /locus_tag="PNS21_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDI5159773.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1603..1833)
                     /locus_tag="PNS21_002072"
     CDS             complement(1603..1833)
                     /locus_tag="PNS21_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159774.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1960..3342)
                     /locus_tag="PNS21_002073"
     CDS             complement(1960..3342)
                     /locus_tag="PNS21_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDI5159775.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3498..4685)
                     /locus_tag="PNS21_002074"
     CDS             complement(3498..4685)
                     /locus_tag="PNS21_002074"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDI5159776.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4692..5723)
                     /locus_tag="PNS21_002075"
     CDS             complement(4692..5723)
                     /locus_tag="PNS21_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDI5159777.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5739..7193)
                     /locus_tag="PNS21_002076"
     CDS             complement(5739..7193)
                     /locus_tag="PNS21_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5159778.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            7368..8273
                     /locus_tag="PNS21_002077"
     CDS             7368..8273
                     /locus_tag="PNS21_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDI5159779.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            8364..9041
                     /locus_tag="PNS21_002078"
     CDS             8364..9041
                     /locus_tag="PNS21_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDI5159780.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            9038..9208
                     /locus_tag="PNS21_002079"
     CDS             9038..9208
                     /locus_tag="PNS21_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159781.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            9322..10218
                     /locus_tag="PNS21_002080"
     CDS             9322..10218
                     /locus_tag="PNS21_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDI5159782.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(10273..11643)
                     /locus_tag="PNS21_002081"
     CDS             complement(10273..11643)
                     /locus_tag="PNS21_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI5159783.1"
                     /translation="MEEKKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11881..12627
                     /locus_tag="PNS21_002082"
     CDS             11881..12627
                     /locus_tag="PNS21_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDI5159784.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12624..13463
                     /locus_tag="PNS21_002083"
     CDS             12624..13463
                     /locus_tag="PNS21_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDI5159785.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13464..14414
                     /locus_tag="PNS21_002084"
     CDS             13464..14414
                     /locus_tag="PNS21_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDI5159786.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14555..15307
                     /locus_tag="PNS21_002085"
     CDS             14555..15307
                     /locus_tag="PNS21_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159787.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            15326..16477
                     /gene="queG"
                     /locus_tag="PNS21_002086"
     CDS             15326..16477
                     /gene="queG"
                     /locus_tag="PNS21_002086"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDI5159788.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5370 a         3092 c         3320 g         4732 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtctc gtccaatttc gtaaaacaac cgtaacggtt
      541 gtgtaaaaat atcattttag agcataaaaa aaaggagtgg atttctccac tccaaaaaag
      601 tctaactttt gggggtcact acagtgtcac agtccttttt ttagtaaaag gatacatttc
      661 ttccttgtat attccatgcg tgttcgtgag aaagcatata taatttaatt aacagatagt
      721 tagggggaga aaaaatgatt ttttcaatta caatgaaact ggtgttcggg ttgatcggtt
      781 tattattagt ggtacgtttg cttgggaaga aatccatgtc agaaatcaca ccgtttgatt
      841 tagtctatac gcttgtttta ggtgggatct tagaagaatc aacctatgat gacaatgtcc
      901 atgtagggca tgtcctattt gccattgcgc tttgggcagt aatgatttat ggtatcgaac
      961 gtatcgtcca aaaaaatgaa aaggtcaatc gttgggtcaa aggcgagcca tctgttctaa
     1021 tcaaagacgg cgtcatcaac atgacagagt tgacaaataa ccatatcgag atggagcagt
     1081 tgcgagcaat cttaagacag caagaatgct tttctcttga aaatgccaaa catgttatcc
     1141 tagagaatgc cggtcaaatg agtgtattga aaaaatcaga tgaagacaaa gctttatcga
     1201 tcctgcttgt agatgaaggg caaattcagc ataaagtgct tcagtccaat caattgactg
     1261 aagcatggct tatggagaac ttgaagaaag aaggctatgc agatgtaaaa cagctgattt
     1321 atgttgaatg gtcagaagaa aaagggctct acatactaaa taaagaagac gtcatgcatg
     1381 aatcttaccg aatagatgga tagatagaca gaggttgtga aatcagcgcc ttggcttgtt
     1441 actcaaaact aagcgacttt ttcacaacct ctgatatcta atatacgttg ttccagaagt
     1501 tgcttcttcg aaaaataagc caaaattcca caaaaattta agaagcaatt ttcgaaaaat
     1561 cctttcttat tttttgaagt tgatcacttc tgtcacaacc tctcatgtca caaaccattt
     1621 ttttgtttcc tttaactcaa tataccgtac gtcttcccac aatatcggct gatggtcaat
     1681 cataaagtaa tcttcatagg cttcaccaat gaagtgccct tcgacctcat ccaagagtct
     1741 tccaaattca tcttttaaat tgagctgaat gattactgct ttacgagata aaaaggcttc
     1801 tgtcagtata ctatcgattt cttgcaatgt catttgttgt ttaggaagag gctgtttgcg
     1861 tgcttcttct tcattttttt gataccgttg actagttctt ccatcgcaaa agcagtcccc
     1921 cattttaatc caaatggccg atctcgatac tcgttatagt caatgaattt ttttgctgtc
     1981 cgtctcatct tttccacctt ctaatccact cataccacct gcatgcccgc caacgagaga
     2041 actacgtgcg atggcgcgtc ctccttgcaa gagactatgc gcatggatga ttgattgaaa
     2101 gccgtatttt tttctaatcg tatcgacaac aaaatcaact ttcgcttcat ttatctgctt
     2161 ttcaggatct tcaaaaaggt taagctgtaa acctgtcgcg tatacgaggt cggaacagtt
     2221 cacgcctaca cgccgaatat cctggtagtg gtaatgctgg tggaaaatgg tcaataaagc
     2281 atctgctaga tctctgctag catttgtcgc ttcgattttc atttgctggt gaaacccggt
     2341 cttgccattt ggatcaagat agcctaaaga gtatcctacc cataagctga ctacttgtgt
     2401 ttttgctgca gatcgtctca agcgagtgcc gacttggtcg gccatttctt tgataacgat
     2461 ctcgatttct tctcgtcttg tataatctct atctaggatt tgactatttc cgatactttt
     2521 tgattttggt gtatattctt gtcccaaaaa acttcgatcg atgccccaag catgggcata
     2581 aagctgagta ccgatgaccc ctagcttgct tttcaagtga taataatccg cgtgagctaa
     2641 atcataaaca gaatgtatac ccatttgttt cagacgtttt gctgttcgtt tgccgattcc
     2701 ccaaaagtca gtgatatctg agattttcca taacttttct ggtacatctt cataacgcca
     2761 ttcagcaaac atttgctttc cgtcttttga gccattgtct aaggctagtt tagcaagtag
     2821 cgggttatcg ccaattccta tagtcgtgta gattccggtc tgctcaaaca catctttatg
     2881 gatcagttta gccatgtcgt acgcatttgt tttaccaaag agtgccatgc tatttgttac
     2941 atcaaggaag ctttcatcga cggaatagac atgatggttc gcttcgtctg catatttttt
     3001 atagatagaa ttgatttctc tgttcttttc catataatag gccatccttg gggcaacaat
     3061 atataaatca cttggatagg ggaagggaag atctctggca cggctgatat tggtgatgcc
     3121 gtaagctttt tttgctgcag gactggaagc caagattaat ccgctacccc gtgtttctgt
     3181 cgaatcggac ggataggaca tgactactaa tttagttgta aggggatcaa gatccttttc
     3241 aacgcactct acggaggcgt aaaaagattt gcagtcaata cataaaatat ctcttgaagg
     3301 ttctttttca taaacaaata ccggattttg attcgcccac ataacaatca cctcttcaac
     3361 acaattagaa cacacgttct ctttccgtgt caataaaaaa cagcaatttt caaatacgag
     3421 caaaaggctc acatttggaa attgctgaga ggttattttt atctatttgt ttttagctga
     3481 gatcgttgat ttttttatca aacatgaaca gcttctggat cgatctttgt tgtcccattt
     3541 ccaatgtgtg caacataaaa ttccggagca tagccgacaa ctttcatgta ttcatcatag
     3601 acattgtttt tgaaggcagg tatattttct tctttgacaa gagcaatcgc acagccacca
     3661 aatccagctc ctgtcattcg tgcacctaag acaccttctt gtttttgagc agcatctact
     3721 agtgtatcta attcgatccc agttacttca taatcatagc gaagagagtg gtgagaatca
     3781 tttaacagtt gaccaaatgc ttctaagttc ccagcttcta attcagcttt tgccttcaaa
     3841 gtccgctgat tttcagaaac ggcatggcga gctctgcgta tcaatgtatc atcgccgatt
     3901 tggtcaatag ttgattcaaa ttcttcttca tctaagtctc ctaaagaaga gataggcaaa
     3961 gttttttgca aacgtgccaa ggcttcttcg cattcgctac ggcgttcatt gtattttgaa
     4021 tctgccaatt cccgccgctt attcgtattc atgatgacga ttgcataacc atctagtttc
     4081 actggaacca tttcgtattt cagcgtattt gtatctaata agattgcatg atctagctca
     4141 ccgaagccga tggcaaactg atccatgata cctgaattga caccgatgaa ttcattttct
     4201 acttttttac ctgtttggac tagttccaag cgatctgttt ttaaatcaaa cagatcttct
     4261 aatacaacac ctactaataa ttctaatgaa gcgctactgg aaagtcctgc accattaggg
     4321 attgttcctt ccactaacaa ctcaaaccca tgatcgattg agtgtccagc ttcttttaat
     4381 ttaaggacca ttccttttac gtaatttgcc cagtttgctt ttttatcata ttctagctca
     4441 tctaaagtaa aactaatgac accgatatcg tcaaagtttg tggaataaac taatatttta
     4501 ttatcgtctc gaggtgctgc tacaccttgc gttccataag tgatggaagc ggggaagaca
     4561 tggcctccat tgtaatctgt gtgttcacca atcaaattga tgcgtcctgg tgagaagtat
     4621 tgtgtagttt ttttaggtcc gaatgtttcg gcaaattttg tgtttaagct tcttttgatt
     4681 tccatgatga gttattcctt tcttgttttg attttgaatt ctgtcactgt ttcaaatact
     4741 gatccggctt ttaaatgaat cgaaccaaat gctgagaagc gctctgcacc tggagctgtt
     4801 tgagtctcaa atgtgatgcc accgtgatgt gccagttttc ggttgcgcat ttctggtgtt
     4861 tctgtgccga aatttgcagt gaaaataacc acactagaag catctgtagc aatacctact
     4921 tggattcttt tatcggggct agtcaagcga gcggcttctt tacccaagcc ggtttctttt
     4981 aagaaaaatg gatgatcgat tccgtcaaca agattttttt gatccaaatc actagcaaaa
     5041 acggacgcta attttttagg tatttgaaaa tcgaaagcac ttcctgcagc attttctttt
     5101 acccctatag gaatactatc tgttcgcaaa ggagcataag cttcgctatt gagccaaagc
     5161 tcatgttgat cgattgactg agaagcatct cccgtcaagt tgaagtaaac atgatttgtc
     5221 ggattgaata aagtatcttg gtcactggtg ccgcgagagg tcactcgcca gatattatct
     5281 tttgtcaaag tatagcggat ttccacatcc attgtacctg gaaaaccatg ttctccgtcg
     5341 ggacttgtag tagtaaaaat gacagaagct tcattttcgc cattcaaaat gacataagac
     5401 cattttttta attcaaatcc aggagcacca ccatgcagat tatgtccagt ttttggatct
     5461 gtggctaatt ggtaagtttt tccattgaga gaaaagcggc cattttcgat tctccctgcg
     5521 gtgcgaccga tagaagcacc gatatacgga tctttttcga tatattcttc agcggtgtca
     5581 aatcccaaaa ctaattctcg atcatttgat tttaaactga cgattctagc acctaaatca
     5641 gagatagaaa gtgctagctt gtttttgttc tccaaggtta tcaagtgata accttggcca
     5701 aaatcttttt ctgtaatctt cattttcaag atcctttctt aatgggaagg aactactgca
     5761 tcgtgaagtt catcttccat tgtatcagct ggagccaatt tgttttccaa ttcctcaacg
     5821 atttgtgcat gttttttctc tgtcaatgta atcttccaag caaaaataat agcggagaaa
     5881 atcatcaaag cagcaggagc atagaatgca taagctttga aagtcgttac gccatgagca
     5941 gtgatatcag cagcagtagc attaccagtc atcccagaag cgatagcgac aaaccctacg
     6001 atcccgttag acaaagcacc agcgatttta tctagtaaag ggcgaattga tagagtaaca
     6061 gcttcgttac ggataccatt tttccattga ccatattcaa cagagtctgt gatcgtcatc
     6121 aaagcggata agaaaatcaa ttgttgaggg aagtagaaca atactagccc gatgataacg
     6181 attggcaaac ttgaacctgc gatcgtaaag aaaaagtaag aagaaatcat cattgcgatc
     6241 cctccaaggt aaacgtagcg acgagagatg acgcgtgtaa gcattgggaa tagtggaaca
     6301 gctactaaac caagtaaggc agcaactaga ccaacgatcc aaaattcagt tggttggcca
     6361 agtacatatt tgaagaagta gaataatacg gcagttgttg ctacattggc aatcgcataa
     6421 gcgatataag caagggcaag ccacatcaac tgatcatttt gagcaactgc tttgaacaca
     6481 tctttgatcc cagctttttc attttgctga cggatcgcag tatcgacttc ttttgtgccc
     6541 aaggcagtga taatcgcagt accgcctgaa ataacagcag caatcacacc gaaccagaac
     6601 cagcctgaag cgccttcaat atgtttatga gtaacaagat aggtaaagaa agtaacgata
     6661 gggacaacga ctagagtagt accatttgca cctagagaag agccgaaacg agcaaacgta
     6721 gcaaattttt cacgttcctt tgtttccgta ctcaaagctg gaatcattga ccaaaaagcg
     6781 atatccttaa atgaataaaa actatccaat aagatgaaaa cgattgcaaa taatatgata
     6841 aataaagtcg tattatgagt agctaagcca aagaaattgg taaagattgc taccaagagt
     6901 acggaactaa caacgccgcc gataactaac catggtttga atttaccgta ttttgtacga
     6961 gtattatcga tgatgccacc aatcaaagga tcaaaaataa tttcaactaa gcggatacct
     7021 acaactaagc ttgtaactgt agcaatcatt tttgcattcg ttgccttatc tgttcctgcg
     7081 aacatctggc ttgtcacgaa taccataaaa taagtgctta atgtaacata gaatgtgtcg
     7141 tgtccaaaag cgcctaaagc gtaggacaca cgttggatca ttttgctttt cataatctgt
     7201 attcctctcc tgttcaatat ataatactaa gtaaaccggt tacaacgaat aacaaagatc
     7261 ttttaatgac ttaaacttaa caagaaatcg cttccttttt aatatatttc tgtgactttt
     7321 atttaacttt ttgttgcatt atactaaagg agtaaggagg gatagacgtg gagcaatcga
     7381 tttttttaaa aaaggaacat caacaagtag aaaaagaatt gtattttgat ttttgtggtt
     7441 tctctaaaac gatgccgttt cattcttttg gtcctgctat tcgcaatgat tacatcctgc
     7501 atgtcgtgat ggaaggcaaa ggatcttatc atgtgaaaaa tcaacaattc caattgaaaa
     7561 aaggtgatct gttcttgatt cgtccaggag actcgacgtt ttacttatca gatggagaag
     7621 atccgtggat gtattgctgg ctttcatttg gtgggagtgc tgcagaagaa atcattcggc
     7681 agtcgctttt cagagaagac caatacacga tggtctcttc tgacatacaa aaatatatcg
     7741 acattatttt agaatgcatg aaagatacgg aagaaacctt ggaaaatgaa ttacagttaa
     7801 cagcattaac ctaccgtttt ttatcagtcc ttttaaaaga tggcggaaag gtcagcggtg
     7861 gtgtacaaaa ggttcattcc tctttagctg ttgaaacagt caaatacatc gaggcacatt
     7921 atgctgaaaa attgtctgtt gaggaaatcg cccagcattt agcggtcaat cggagccatt
     7981 tgtcgcgagt attcaaaaat catatgggta ccagtatcaa ggaatatttg atcggggtac
     8041 ggatcaatcg aggggcgttt cttctttctc tgacggatga atccgtggaa agtattgcct
     8101 atcaagtagg gttcaatagt ctagtcgtat ttagccgaat gttcaaaaag agtaccggag
     8161 aaacggctac gaactatcgt aaacgtatga aaaacgaaaa ttttaagaat attgctttgg
     8221 ataccttgaa aaaacagttg gaaaaacaaa ccgcagtttc ttggacaacc tgattagtct
     8281 ggttaaaatg acaagcatcg gcaaaagaaa cttgtggcgg ttatgctaca ctgtaaagcg
     8341 aagactaaaa aggaggaaaa gggatgcgta cacttatcat cattagccat ccaacgatcg
     8401 aagaatcttt aaatcagcaa tttttcaaag aagcttctgc tggtttacca gctacttggc
     8461 atcatttgga atcaagttat ccagacggaa aaatagatat agagactgaa ctacagctct
     8521 tggcccagca tgatcggatc attttccagt ttccgtttta ctggtatagt tctcctgctc
     8581 atttaaaaat ttggcaagat aatgtcttgg aacgagcagc acatgtatta aaagggaaag
     8641 aactaggaat cgtattgaca acaggtgtgg cggaaaaaga ataccaagct ggaggaaaag
     8701 aagaatatac gatttccgaa tttttgaggc cgtatcagcg aatcgcaaat aaattccata
     8761 tgacctatct tccgccattc gttttagcac aatttatgta tttatcccag gaaaaacgtt
     8821 gggagaagct aattgcttat cagcagtatc tctcattgga aggcaaacca tcgctgactc
     8881 agcggattga ctggtttatt caaagagtac aggaaaatca gaaaatgcag gaagaagaat
     8941 ctgaaaaaca agcatatatc gtagaagcac tgactgaggc aaaagagcag atcgaagact
     9001 tgacctttac actgcaagaa atgaaaggaa cttcattatg acaaatgatt ggctgatcca
     9061 acaattagat gaattaagcg aaaaatcaga atatcaagac cgtgcgctct tatatgaaat
     9121 aaagaaactg atggctgaga tagacgaacg aacaatgcag ctgcaaggag aaatcgacgg
     9181 acaatcttgg aatcatctca actggtgaaa ttttcttata aataacaaag ataaaaatgt
     9241 gcacagcgac aattatttgc tacaataaga atatgaagcg tttgataacg ctattataca
     9301 taaaaattgg tggtgaaaaa aatgtatgat gaaaatgagt ggggagaaaa tgttcggatg
     9361 acccgaagaa gacagcaaaa acaggcagct cgtaagcgga tgatcatgat tggggcagca
     9421 gttgccgtgt ttttgatcat agcaggagga gttgcgggtc tatattttca tggaaaaaat
     9481 tcgtcagaag cgaaaagtac gaccataacg gaaacaacga caaagaaaac agagacgaaa
     9541 caaacaagtg agtcatcaac taaaacaaaa gagacacaaa cttctgaaga aaaaacagca
     9601 aatggtttgc cagagcaact tcaagcaatc gtaaatgatt attcgaaaaa gatcgaagaa
     9661 aagacgcctg cattgattga agaataccag acagaaattc agggaaatca agagggaata
     9721 gctggtttat cagcagttgc taaccaaaaa gcacgtgagc ttcaagcaat ctccgacgaa
     9781 gggatcagaa aattacgtgc agcgtatcag gcagcagaaa ataaagacgg tgtagactta
     9841 gatactttga tcaatcaatt atcggctaat tatacgaatc atgtcgctaa gatttccgat
     9901 atttatttgc agactagcgc ctctcttcaa gcagagtcaa caagcacaca agacacaaca
     9961 tcttctgatt ctgagacagc agagtcggta gaagcttctg aaatgcaagc tcgaacaaca
    10021 caagacacaa cagattcctc tgaaactaat accaacagcg cgacttcaca ggctgcgaca
    10081 accgtagttt tggaaggaga aggtccaaat caaatagccg aacgaactgg cgtaccagta
    10141 gaaacgatct tgtcgctgaa tgggatgagc atggagaatt atttcctgac tcccggtgag
    10201 acgctaaaat tgaattgata aaagcaataa aaaagaacga agcttccact aaggtgaagc
    10261 ttcgttcttt ttttattttc cggttctttt ttcgcgtaca aagtaatgga tcgtcatgta
    10321 aagcaagaaa acgagtccaa taatcaatga gattctcgtt tcaggattga taaacatgaa
    10381 aatcaatacg atagctaaaa aagcaagtgt cacataattc gtataaggag atagtggcat
    10441 tttaaaagga tgatcatgca ttttatctgc gtgcttcttt ttgaagcgaa tctcgctgat
    10501 caaaatcact acccaaggaa ccataccagg caaaatactt gagctgtata cgaaaacgaa
    10561 aatattttct gactggttga tgaacattgg tagaagataa tttaagatca gaccaattaa
    10621 gattccacct gagattgcta cgacaggata aaaaggcgca ccgtgacgag atagtttcag
    10681 aaatttttta gacatctttc catttatggc aagtgtatag accatacgac ttgcgctgaa
    10741 aatccctgaa ttgcaacctg ataatgccgc tgtaatcacg acaaaattaa tcagtcctgc
    10801 agcaaatgta atgccgactt tagaaaaagt ttgtacaaaa ggtgaaccaa tctcactcaa
    10861 ttgattccac ggatagatac tcacaatgat aaaaatagca ccaatataaa aaatcaagat
    10921 tcgaccgata gtggactgga cagcttcaat gatcgtcttt ttaggattct ctgcttcacc
    10981 agcagtcatt ccgatcagtt cgattccttg ataggaagca acaacgatcg ataatgcaaa
    11041 gaaaaatcct ttgacgcctc cagtgaagaa tccaccattt ttccaaagat ttgaaatccc
    11101 tactgcctga ccatgattac caaatccaaa taagattacc ccaagacctg caatgatcat
    11161 caaaatgatc gttatgactt tgatgatcga aaaccaaaat tctaattcgc caaacgcctt
    11221 gacagaaata agatttgcca gcataagaaa caagacgaca atgattccgg gaataattgc
    11281 cggcaaatca ggccaccaat aattcatata agatcctaca gcaatcactt cacttattcc
    11341 tacgacaata tattgaaaaa tattactcca agcggtcaag tagccagcca caggatggat
    11401 gtactcgctc gcgtaatttg caaaagaacc agtcgaagga tcaacgtaaa gcatttcgcc
    11461 taaagcacgc ataatcaaat acaaaaagaa tcctgcaatc gcataggctc ccatcactga
    11521 gggccctgtc caacgtatgg ttgaggaggc acccataaac agaccgacac cgatcgttcc
    11581 acctagtgcg atcatttgga tatgacgggc actaagactt cgagttaact ttttttcttc
    11641 catacaaaaa ctcctttcgt tgttgagtca acaagaaaaa ataatcaaac gtttcaacac
    11701 atgtttttca ttattaggtt agccttattg aactcttgat gtgattctac agaaaatttt
    11761 cataaaatgc gaggaaaatt cgaaaaaaac tttttcatca ttctacttga aaatgctttc
    11821 cattttagtt aaactatatt taaataaaaa gttaggtaaa cctaaaaagg agtgtaagat
    11881 ttggaacgta cggccattgt aattcaagac ctttcagccg cttatcaagg gaaaactgtt
    11941 ttgaaaacta tcaacttgac gataaatcca caaaaaatta ccggtattat cggaccaaac
    12001 ggtgcaggaa aatcaacatt tatgaaatct ttgctagagt tgataccagc tactactggt
    12061 aaagtcacct tttctggaca gccggtaaac tcagtccgta aaaaaatagc ctacgtggaa
    12121 cagcgaagtg aattggatct ttcctttcca attgatgtat taggcgttgt acttttagga
    12181 acatatccat ctttacgaat tggacaaaga cctgggaaaa aagaaaaaga acgtgcaaga
    12241 caagctttga aaaaagtagg gatggaagaa tatgccaaaa gacagatcag cgaactatcg
    12301 ggtggacagc ttcagagagt ttttattgca agagctctag cccaaggagc agaatggatc
    12361 tttttagatg aaccattcgt agggattgat gcgttaagtg aacgaaagat ctttgacatc
    12421 ttgcaggaat tgaagaattc aggaaaaacg attttgatcg tccatcatga tcttcataaa
    12481 gtagaagaat atttcgatga ggttattctt ttaaataaac agctgatcgc ttccggtcca
    12541 gtacaagaag tctttacatc gaagaacctt caattggcct atggtgagat catcagacat
    12601 ttagtaaaag gaggagagaa aaaatgatcc aatcctttat tgatggatta atgaattatc
    12661 aattcctgca atacgctttg gtcacttcca tgcttgtagg acttgcttct ggagtcatcg
    12721 gttcttttat tattttacga gggatgtctt tgatgggaga cgctatctct cacgcagttc
    12781 ttccaggtgt agccatttct tatatgtttg gttttagtta catctttgga gccactgctt
    12841 ttgggatgct aacagcagca gtgattggtt tcgtgactca gcggagccga ttgaaaaatg
    12901 atacagcgat tggaattgtc tttagctcct tttttgcatt agggattatc ttgatttcat
    12961 atgcccagag tgcgacagat ttgtatcata ttttatttgg gaatgtctta gctgttaggg
    13021 aaagcgattt gttgctgaca gcactcgtta gtggaatcgt cttgatattt gtctttttct
    13081 tttataaaga attgaagatc acatcattcg atccgacaat ggcaaaagct tatgggctga
    13141 acacatcgct gatccattat cttttgatgt tctttttgac attagtggct gtagtcagtt
    13201 tacagacagt aggaacaatc ttggtgattg ccatgttgat cacaccagcc gccacggctt
    13261 acttgctaac gaaccattta ctgaaaatga tcattacagc tgcaggaatc ggtatgctaa
    13321 gtgcagttgt cggtgtgttt ttcagttata gttacaattg gccatcagga gctacgatcg
    13381 tgttagcatg taccgcattt tttatccttg cttttttatt ttctccaaca aaaggaattt
    13441 tatttcagaa aggaagcaga tagatgaaaa caaaaaaatc actcttttta atcttggcag
    13501 ttagtttctt ggtattagcc ggttgcggaa agcaggcttc tgaccaagcg gatgaaggaa
    13561 gtaaagaaaa gttgtcagtc gtggctacca attcgatctt ggcggacatg gcaaaagagg
    13621 tcggaacaga tcaaatagat atccacagta tcgtaccagt cggaacagat ccgcatgaat
    13681 atgaagtact accagaagac atcaaaaagg caagtgatgc agatgttatt ttatacaacg
    13741 gtttgaatct tgaaacaggt aacagctggt tcgataactt gatggaaacg gctaaaaaag
    13801 aagaaggaaa agattatttt gcagttagca aaaatgtaga acctctttat ttaactagcg
    13861 gtgaagaaca tacaaaagca gatccccatg cttggctaga cctatctaac ggaataaaat
    13921 atgtggagga aatcgcacgt atattctctg aaaaagatgc agaaaatgcg acactctata
    13981 aaaaaaatgc agaagcatat gtggaaaaac taaaagaatt agatacacaa gcgaaggaaa
    14041 gttttgcttc tatcgaagag aacaaaaaat tattagtaac aagtgaaggg gcattcaagt
    14101 atttttcacg agcatatgat ttgccagcag cttatatatg ggagatcaat acagaaagtc
    14161 aaggtacgcc tgatcaaatg aaagcgatca ttgatcagat aagagcaaaa gaagtaccgg
    14221 ttttattcgt ggaaaccagt gtagactcaa gaagcatgga acgggtagca aaagaaacgg
    14281 gtttgaaaat ctatgataaa ctgttcactg attccatagc aaaagaagga gaacaaggag
    14341 attcttacta tcagatgatg aaatggaata ttgaaacgat ccatgaaggt ttgtcacaaa
    14401 caaaagaaag ctgaaaagtg caaggtctag cttgaggagc agaccttgca ttttttataa
    14461 gagatatgaa aaaaaattat gctgtttttc gtcgtacaga tacctttttt gaagaaattc
    14521 cgttatagtt agagtaagga aggaaggtgt ggctatgaat tttacacgta aagataaata
    14581 tacgattaaa gaagaagtct ttcgattgat cagccagcag gaaaataccg acaaggatca
    14641 agaccttcaa tttcttgaag cagaattagc taaattattc atagcaaatc aaaatgagac
    14701 aacagaacca gatgaagagc tgtacatagg caaatggttg tttgatgaat ttcagccttt
    14761 agatgaaaat accggtatcg gttattttgt gttcctttct ccagtcggct tcacacctgg
    14821 cgtattagtt aaacaaaacg acgaaataac cgtcaaagca tggcctgcat tattttcagt
    14881 catcaaagca gaaggagtaa tcaaaaagat ccagtcggcc tattataaaa gtcaatttat
    14941 tgccaacagc aaattaatga atatgtttta taatcgtcta gtagaaattg ctaggcctgg
    15001 gaaaatcccg caaacattca cgtacacaca gcaaaaaaat ctctatgaag aagtattgta
    15061 ttcccgcgat ctgtctgagt tttcgccatg ggaagaggta aggctccatg aactgttaag
    15121 tacaaaacca gtttcattga atttttttaa tgcattagat aacaaaagaa aattaatttc
    15181 aacaaattat tgggaagtat ttgctgcaag aaaaaaatgg atggaagaaa agaagattgc
    15241 cgatcagcag atccagtttg tccagctgaa aaaagaaaaa ctatattatg ggttatacag
    15301 ccgttaacag caagcgagga agaccatgac attaaaagaa gaaatcatac aagaaagcaa
    15361 acgattagga attgacaaaa tcgggttcac cacggcggaa ccttttgatt cattaaaaga
    15421 atcgttggaa gaacaaaaag cagctggcca tacttctggc tttgaacatc caaatatcga
    15481 tgagcgtctt tatccagaaa tgacatttga tcagccgaaa tcgatcattt ccattgcact
    15541 tgcgtatcct actaggatcc atgaagaagt gcctagggat gaaaaacgcg ggcagtttgc
    15601 tcgtgcttct tgggggatcg attaccatga tatactcaga gaccgtttga accgattgat
    15661 cgaattcatc caaacaagag cagaaaaatg gcaaaaagaa gaggaatggc gtctagctcc
    15721 gcaagtagat actggagaac tgatcgatgt cgctgtagca cagcgagctg gtttaggatt
    15781 tattggacga aacggattgt tgatcactga agagttcggt tcatttgtct atcttggtga
    15841 gatcgttact aatattgtat ttgagcctga taaaccagga gaatttggtt gcggtgactg
    15901 tatgcgctgc gtgactgctt gtccgacaaa agcattgctt ggtgacggaa gaatgaatgc
    15961 ccagcgatgt ttgtcttatc aaactcagac aaaagggatg atgccggaag aatatcgtaa
    16021 aaaaatgcga aatgtcattt atggatgtga tatctgccag ttagtctgcc cttacaatca
    16081 aggaaaagat tttcattttc atgaagagat ggaaccaaaa gtagacgaag tatatccaaa
    16141 attaaagcca atgctttcga tgtctaataa agacttcaaa cagcaatttg ggcatttagc
    16201 tggttcatgg cgtgggaaga aacctttgca gagaaacgca ttgattgctt tagctaatct
    16261 aggtgatcga acggcattgc ctgagatttc agaatgttta acagatgtcc gccctgtcat
    16321 cagaggaaca gcagcttggg ctattggacg tctaggaaca aaagaaccgg aaaaatggct
    16381 tgatgtatta gatgaaatat tagctaaaga aacagaagaa gtcgttataa acgaattgga
    16441 acatgcccag aaattattgc ggagaaagtt aaaataaaaa aatagttaac attattcaaa
    16501 gtttttgtcc agta
//
LOCUS       DAMYXM010000058        15939 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.059, whole genome shotgun sequence.
ACCESSION   DAMYXM010000058 DAMYXM010000000
VERSION     DAMYXM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.059"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(173..475)
                     /locus_tag="PNS21_002087"
     CDS             complement(173..475)
                     /locus_tag="PNS21_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159789.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(695..1061)
                     /gene="ssrA"
                     /locus_tag="PNS21_002088"
     tmRNA           complement(695..1061)
                     /gene="ssrA"
                     /locus_tag="PNS21_002088"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1232..2005)
                     /locus_tag="PNS21_002089"
     CDS             complement(1232..2005)
                     /locus_tag="PNS21_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDI5159790.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2149..2499
                     /locus_tag="PNS21_002090"
     CDS             2149..2499
                     /locus_tag="PNS21_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDI5159791.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2480..3196
                     /locus_tag="PNS21_002091"
     CDS             2480..3196
                     /locus_tag="PNS21_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI5159792.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3196..4644
                     /locus_tag="PNS21_002092"
     CDS             3196..4644
                     /locus_tag="PNS21_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDI5159793.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4714..5223)
                     /locus_tag="PNS21_002093"
     CDS             complement(4714..5223)
                     /locus_tag="PNS21_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159794.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5213..5938)
                     /locus_tag="PNS21_002094"
     CDS             complement(5213..5938)
                     /locus_tag="PNS21_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159795.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6161..8281)
                     /locus_tag="PNS21_002095"
     CDS             complement(6161..8281)
                     /locus_tag="PNS21_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDI5159796.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8511..9689
                     /locus_tag="PNS21_002096"
     CDS             8511..9689
                     /locus_tag="PNS21_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDI5159797.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9705..10463
                     /gene="aroD"
                     /locus_tag="PNS21_002097"
     CDS             9705..10463
                     /gene="aroD"
                     /locus_tag="PNS21_002097"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDI5159798.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10486..10971
                     /gene="ybaK"
                     /locus_tag="PNS21_002098"
     CDS             10486..10971
                     /gene="ybaK"
                     /locus_tag="PNS21_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDI5159799.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(11042..12295)
                     /locus_tag="PNS21_002099"
     CDS             complement(11042..12295)
                     /locus_tag="PNS21_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI5159800.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12412..13761
                     /gene="gdhA"
                     /locus_tag="PNS21_002100"
     CDS             12412..13761
                     /gene="gdhA"
                     /locus_tag="PNS21_002100"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDI5159801.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13873..15219
                     /locus_tag="PNS21_002101"
     CDS             13873..15219
                     /locus_tag="PNS21_002101"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDI5159802.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15527..15913)
                     /locus_tag="PNS21_002102"
     CDS             complement(15527..15913)
                     /locus_tag="PNS21_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDI5159803.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5216 a         2844 c         3192 g         4687 t
ORIGIN      
        1 cttataattg gagtggtagt taattagtga cttatttttg attttatagc actgatacta
       61 taaaatatag atatcatcat attacacaat cttaatacta acttaaaaaa tatccctttt
      121 ataagtatgg tgataaaatc cgttccgggc tacctttttt aggtagccta ctttaatctt
      181 tatacctttc tggatcaacg aaagtatact tcaaatagtc ataacgccga tgatcgctcc
      241 gtgcgtctgg cacatcagtc acgatatcaa acaaaaaata cacatctttc ttcattctgg
      301 tttttgttgc cggaatttta aagtagttct tattagagta atagagattg attaataagc
      361 tatcttcgat tgctaaaaag aaaacttctg aatcccacac cttataaaaa tctttgacaa
      421 atctattcga aggatcaaat ttaaaccata attgtgtttt tccttccatc aacataatgt
      481 tcacctcaaa aagagtatac gaactaatgt tctttttgta aagtaggaat atcattgata
      541 atatggatgg aacatatata ttaaaaagtg ttctttttga atgctatttt gaatgccatt
      601 ttcattcatt taacgaaaat ctgcgaaaat aaaaaatgct caaaccctta taaaataagg
      661 atttaagcat ctagcgaaat ctgctgaaat ctcacaatgg agacggcggg agtcgaaccc
      721 gcgtccaaac acatcgccac ttaaaaatct acgttcatag tcaactcatt taaggattca
      781 ctttacagct tgccgagtga caggcattct gtatcgctag tctgatgttc tctttttcaa
      841 cttacagacg gaaagttgaa acgtatccca ctaaagttag gacccttact cgagcacatg
      901 ggcgatgccg agaggatcta cgctaactgt ttttaggcag ctaaagcgta agagttgttt
      961 tcgtttttag cagttatatt taactgtaac gtttttacgt agacgtaacc tacgaaacgc
     1021 aattcaagct cgactgtgcc tgtcgaatcc gtaacgtccc cgttagctaa caagggttac
     1081 gtgtattttt catacaaaat tagtatagca tatattggga taaattcaat aaaaaagcga
     1141 atcatatccc attatttata taataactgc ccctttctta agaaaaaata agcgattttc
     1201 ttaagaaaag aggtagcttc aaaaaaagat cttaaatcag atcaaaatac ttcaatgcgt
     1261 ggacaattcc gccttctgta tttttctttg tgataaaggt agaaagttct tttaatccgt
     1321 cataggcgtt gcccatagcg attttgttgt cgcaagcttc caacaaggca aaatcattcg
     1381 gaccatcgcc aaatccaaaa gtaggaactt tttccagctg aagttctttt tgcagacgtt
     1441 tgacacctgc cccttttgag attcctttct tcactgtatc gatcgagtaa ggaccattcc
     1501 gataaaaagt tagctcagga aaatgttcgt gatagtattc gtcacctgat tgagataaga
     1561 tcagcaacat atttactgtg ttttctttaa agcctaaagg atcaatttcc ggaacatctg
     1621 aatgaatgaa gtcgtaagca ttgatcattg tttggctatg accggtacac cagattttct
     1681 gatcgttata gtaagaaagt tcatgaccgt tcaactgtac atgatcatac attttttgac
     1741 attcttctga agaaaatgtt tgagaataga tttcttcgcc gtctactcga atgaaagaac
     1801 cattcatggt gaccgcagaa gtaatgcccg catcttccat gatagagtgg atttctgctt
     1861 ctgtacgtcc tgtagcgatg acggggagaa cattgttttc ttttaatgcg ttcatagctt
     1921 ttgccacttc gggtgtgatt tttgatttcg catctagtaa tgttccatca agatcaaaga
     1981 aagtgattgc acgataattt gacacaaata aaactccttt tttattggaa taagtttatc
     2041 ttaacaaaga atgaacaaaa gacaaacctt tcgtgaagaa ttcctgaaca tattggttca
     2101 gtcaacaatt tagagtatac tataagaaga gaatttagga gggaaagaat gatttttttc
     2161 agtattggta tcttattctt aataattggc agtctttttc ggattctgcc ctcaaaagga
     2221 aatctgcctt tttatgggta tcattcgcct cttgccgcaa aaacagacgc tcattggcgg
     2281 ctggctcaaa agacaagcgg aaactggttt tttcttatgg gcttactgat ggcattgatc
     2341 ggctattatt taaagacatc tgggcatacg aattattttc tgattgaaat gcttctgcta
     2401 gtatttccaa ttatgcctat ttttattatg acagaaaaga aattacagaa atatgacctg
     2461 gaaactggag ggaacgataa tgaatatttt aatgattgaa gataatcaat ctgtttctga
     2521 aatgatgcag atgtttttct taaatgaagg ttgggaagca actttcaagt atgatggaaa
     2581 agaagggctc gatgcctttt tagaaaaccc acaacattgg gatatgatta cactagacct
     2641 gaatttgccc acactggacg gtatgctagt aggaagagag atccgcaaag tttcaagtac
     2701 tgtgccgatt atcatgctga cagctagaga ttctgaaagc gatcaggtga tcggcttaga
     2761 gatgggggca gatgattatg tcacaaaacc tttcagtcct ctgacgctta ttgctcgaat
     2821 gaaagctttg catcgccgta gtgagttggc agaagcaaga gaagaagcag tagtttcaga
     2881 tgatcatttt gatatcgaga ctagtcattt caagatgaat acgaaaacac gagaagcgta
     2941 tctggacaac cggccaattg aaggactgac gcctaaagag ttcgatctat tgtttacttt
     3001 agcaaaaaaa ccacgtcaag tattctctcg ggagcagttg cttgaattag tctgggatta
     3061 tcagtacttt ggtgatgaac ggacagtgga cgcccatatc aagaaattac gacaaaaaat
     3121 cgaaaaggtc ggaccacaag tcatccaaac tgtttgggga gtcggctaca aattcgatga
     3181 ttctggtgtt gcctaatgcg ttatttgtat cagcaactct tagctttctg cggaatggtc
     3241 gcgttgatct tattgatcgt tggagtttct tttacacaac tgacaaaaca aacgattgaa
     3301 gaaaacaatt accagcaact gtttggatat gctgaatcag ttgaaaagac aacgcaaacc
     3361 tatgccgata cgctcccgca gtttagtcaa gatcaggcgt ttcaaaatgc attgctgctg
     3421 acagagcaag tactgacaga acaaaatgtg aactttatat ttatcgataa aaacgaacgt
     3481 gtcatttatc cgactaatac agctttactg aattttacct taacagctga tcaatggaaa
     3541 aatctgagga atggacgtca agaaaaattc acatcgaata aaaatatttt aggacaagca
     3601 caggcgacct cttacgcact tgtgccattt aatttgaatc atgaattcta tggagcactt
     3661 gtagttagtc aacctgcgcg aaatatcgat aacagcgttc gtccaattac actgaacctg
     3721 ttcaaaggat ttattttttc cagtatcgtt gcagttattg ccagctatgc ttttgcagca
     3781 ttccaagtta agcgtatcaa tcggttaagg aatgcgacga aagaagtgac gaatggtaat
     3841 tttgatgtac agctccccgt acatgacaaa gatgaatttg atgagttagc agatgatttc
     3901 aataaaatga cgaattcatt gaaagaatct caagcagaaa ttgaagaaca agaaaatcgt
     3961 cgaagacaat ttatggcaga tgcttctcac gagatgagaa cacctcttac gacaatcaac
     4021 ggacttctgg aagggttaga atacaatgca atccctgaaa atcagcgaga aaacgcgatt
     4081 aaattgatga aaaatgaaac agagcgtctg atccgtttgg tgaatgaaaa cttggattat
     4141 gaaaaaatcc gtacgaatca aatcagtatg gtcattaaaa aattcaatgg aacagaaaca
     4201 ttgaaaaata ttgttgcaca gttagaagct aaagcagaag cagcaggaga tacattgaca
     4261 ttgaaagccg ttgatgatat tgatgtttac gctgattatg accgatttgt ccaaatcatg
     4321 gtcaatatta ttcaaaacgc gatacagttt actgaaaacg gccaaatcac gattacttta
     4381 gaaaaaggct atcttgaaac gatcatcacg attgaagata cgggaattgg aatgtctgaa
     4441 caacaaatga aaagtatctg ggatcgttat tataaagtag atccttcaag aaaaaataca
     4501 aaatatggtg aatcaggatt aggtttacca atcgtgcagc aacttgtacg cttgcataaa
     4561 ggaaaattag aagtagaaag cgaactagga aaaggaacaa aattcgctgt tcgtttgcct
     4621 gacgtagaaa tcaaaaaaga gtaaaaaaca agcggtataa aagatcatga atcgatcttc
     4681 tataccgctt gtttttacta taaaatgatt gatttagccc gcgggctcta aacgatcaat
     4741 ataaggactt tgagcaatag tagcatcatg agtaaagagt aagatacctt gattttcttc
     4801 atgagctaaa taagaaagca agtggagtag ctcttggatc tccaatacac taagttcatc
     4861 aaatgtatct tcaataacaa tattgtttcg cttggaaagc aaggcatgaa gtaattgcag
     4921 ctttactttt tcaaaagacg tcaactcagc tgccgcacaa ttaagcaagg aaggagagat
     4981 atcaagtttt tcttgatagg ccaaacgatc ttgtttgatt tctttttccg gtacatccaa
     5041 caggacatgg tctttaagat tcagataagg aaacatcggc caattttttt ggatgatcgc
     5101 aaaattatcc agttttgcaa caagaacctc ttttttcaag tgctccaata ttttatgctg
     5161 tttttcgaga ggacacaaaa taacggtcaa gtgttcagca tgaatcaaat attcaggttt
     5221 cattacgtcc aggcctccta ctttgcaaat gatggataaa aaaagtgaaa gggaatagac
     5281 taagaatgag acagcatcca ctgagcccaa taagagtttg ggcaaatccc ttaagtatat
     5341 ctgtgaaaaa aagatcattt tggaaatcga tggtaaaaaa agtctgttga tccatcggaa
     5401 ttgtaagtcc gcttaccgaa gtcaagatat cacggatatc tggcagttca gtagtcttta
     5461 agacaagaaa attgatcctt tgaagaagcg cttcataaac tgactggaag cttacgatcc
     5521 acaataaaac caagatacaa caaagaaaga gaggaaagaa cagctgccag aaaataaaaa
     5581 ggaataattt ggttttagaa agaccagtca ggtgccaatt gatcaattcc tgcttgtttt
     5641 gcctgactga atagtaaaag aaaaagataa aaaggaaggc aaatagaaaa aataaactga
     5701 atatgccttt tatataaaat cggctagcta attctaaagc cggaagaagc tctgtagatg
     5761 cagaagcgcc aataaatgaa agccgatctt ggacttgatt ggctaaagcc gattgcaaat
     5821 ggtaaagagt caggcatgct atcaataaaa aagcagaaaa gcttgaaaat aaacaataga
     5881 gtattgtctg ctttttgtaa taaattaatc gagcagtagc atagcggatt attttcatac
     5941 ttttcacctc tataaaaagt atagaaaatg aatatgaata aagtatgaaa ttcctttagt
     6001 gaaatcacga attaaaacca gaaaatattt tgtaaaaagc aagaggttgt gaagaagctg
     6061 ccctattcta ttaccgtaga tgcaagcttt tgaacactgt ttattcgaaa agaggagcag
     6121 cgacaataat tttgtcgctg ctccttttaa atttatttta tcattcggaa gaacttgtgg
     6181 tcgttccgtg aagttcttgg tacgtttgcg ttttataaag atcaacagtt gattgtccgt
     6241 tgtgttcact aaagacacta gttgacttgt caccaagaga tttttcagct tcttttaatt
     6301 tctctaagcc attcttgtag tcataatcac tggtatcaat tgggtctaat ccactattcg
     6361 tatagaaccg aagaagatca ccattattga tttggtcaga aacagctaac tgtttatcaa
     6421 ctttttcttt cagtgaatca atttcattct gtacttcctc agtaggttca gtaatagctt
     6481 caccagtttc gttactatag atcgtaccac cgtagtaagt atattttggt gtaacaaaat
     6541 caccatcacg gaaagaaaca agctgttcat gatctttcga gaatagatct tgtcctaact
     6601 gaatataatt ttttgtatct acacctagta agtgaagtaa tgtaggcaag gcatcaactt
     6661 gtccgccgta agtgtgattg attccgccct gtgtttgtcc agggatatgg atcatataag
     6721 ggacacgctg catttgtgca ttgtcgtagt ttgtccaagt agaacttgtt tttccaacta
     6781 attcagctag atttttattt cttgaattcg agacaccgta atggtcaccg taaaggacga
     6841 tgactgaatt atcatacaag ccgctttctt tcaaataatt gaagaattct tgtacggcgg
     6901 tatctaaata attggctgtc gcaaagtaac cgttgatcgt ttcatcattt gtattggcta
     6961 acgggaatcc tgcttcgtca ttggtgaatt ttgaataagg ataatggtta gatacagcaa
     7021 tgaattttga ataaaatggt tgttgcagac gttccaagta ttgtacggat tgttggaaaa
     7081 atggtttatc atgcaacccg tattggaacg aattgtcatc attaacatca taataagaag
     7141 aatcaaagaa ataatcatat cctaaattct tgtatgtctc gtttcgattc cagaatgtac
     7201 cagcgttccc atggaatacc gcactaatgt aaccttgtgt ttgacttaaa atatcaggtg
     7261 cagcttggaa agtatttttt ccgcctaatt gtgtgaacaa ggagccttga ttcaagccaa
     7321 acaatgaatt ttccatcagc gtttccgcat cgcttgtttt tcctgctttt acttgatgga
     7381 aaaagttgtc aaaactgaac gtgctgttgc tgtggtaaag actattgatg aaaggtgtaa
     7441 cttcatgctg gagaccattc tcatctgtca atttataatc gatcaggaat tgctggaagc
     7501 tttctaaatg gatataaatc acattttttc ctctagcgag cccgaacaaa tcgtcatttg
     7561 gtgctgcata atgttctttt acataatttt ctacttcttt catatcattg ggacttgctt
     7621 ctgcccgcac ttggtttgtc tggtaagttt gaacaccgtc ataaaccatg aaagcattca
     7681 agccaagata tttgaccaaa taatcacgag agaatgttcg agtcagcaat tctggacgat
     7741 cagcttccgc cataaaaaga tttcctgaga aaatcataac tgctaatgta gagatcgcaa
     7801 aagccattct tgctcggatc gggcgagggt ccaacttgat tttttttgca atcagcagaa
     7861 cagtcaagat aataaaatcg atccaataga tcacatcata tgggcgaaac agtcgaatgg
     7921 cactttctcc cagaccactc gctactttcc cagcacctaa aatcgtgttg atcgtaatga
     7981 agtccgtgaa ttctcgataa taagtgacat tcgaaaaaac aagcaatgtc aaaagaaaat
     8041 aaatgaccat catcgtgata taagcaactc tagggcgtct gatataaaga gcaattccaa
     8101 gtaataacaa tgtagttgca atagggttga taaacaagat aaagtattga atgccacttt
     8161 cgattccgag atggaaatca actaaatagg caaaaatcgt tttcaaccaa agtaaaacga
     8221 ctagtaaaga aaaaaatccg agcctcttat tcaaaaaggc aggtgttttt atacatttca
     8281 agataatctt cctttctgca cttcttaatc acagaagtgt cctgaaacca tgttacacag
     8341 ctcacttatt ctcatttatt ttactagtat gacaatatag tgtcaattga aaccagaagt
     8401 ttatgctgtt tcattaaggt tttctttaag cttcatgaaa aaatgagcag taagacaagt
     8461 cctatgtttt ttgttatact tgggaagaga aagctaaagc aggtgaagga atgaaagtaa
     8521 aagtaaaaaa aagagcaagc aatcgattga aaaaaggata tccactgatc caaaaagaag
     8581 accttgctca tccccaaaaa gaaaccacgc aatgggtgga attagttgat tcgcaaaacc
     8641 aatttatcgc aaaaggatat ctaggaaagc agaataaggg aatcggttgg gtattgagcc
     8701 agcagaacat cccatttgat caatcttttt ttgaagaatt ttttgtgaag tcaaaagaaa
     8761 aaagaacttt ctttttttca gatgattcta ctaccgcttt tcgtatattc aatggagaag
     8821 gagacgggat cggcggtctg attgttgatt attatgcagg ttatgctgtt ttttcttggt
     8881 ataacgaaac gttatatgca catcgtgaat tattgattca agcttttcgt gaaatctacc
     8941 cggaaatcat aggaggctat gagaaaattc gatttgaatc agtaaatctg cctgagagcc
     9001 agttcattta cggaaaagag gcaccggaac ccttgatcgt aaaagaaaac ggagtaaaat
     9061 ttgccgttta tttaaatgaa ggattaatga ctgggatctt tttagaccaa aaagaagtac
     9121 ggggatcatt agcagaaggt ttcgcttctg ggaaaacagt tttgaacatg tttagttata
     9181 ccggcgcctt ttctgttgct gccgcaatgg gaggggcaat cgaaacaaca agtgtcgatc
     9241 tagcaaaacg cagtttaaaa aaaacacagg aacagtttga agtgaatggt ttggatgtcg
     9301 atcagcaaaa aatcattgtt atggacgtat tcgagtattt caaatacgcc aaaagaaaac
     9361 aatttttata tgacatgatt gtattggatc caccaagttt tgccagaaat aagaaaaaag
     9421 tctttcgcgt agcaaaagat tatggggaac tggtcaagga tagtctggat attttagcag
     9481 aagacgggat acttattgcg tctacaaatg ctgcgaatgt ctcgatagaa caattccaaa
     9541 caatgattga agaggaattc catgccaaaa atgtttcttt tgaacaactt gctttatatc
     9601 gtctgccaaa agatttccag acgacggata cctttttaga aggcaattat ctgaaagtgt
     9661 ttgtttatca aattaaaaat aaccattaga aggagcgatg cagaatggtt cgggtaaagg
     9721 gattgaatat aggaagcggt agaccaaaga tttgtgcgcc tcttatcggg gccgtccgag
     9781 aggaagtatt acgtgaagca gttcttgcac gtgaagcagg ggccgattta gtcgaatggc
     9841 gagtcgatca ttatcgtgaa gttttttctt ttgatgaagt agcagaaaca ttggttctta
     9901 tccatgagat gcttgatgga ctgccgttat tatttacgtt tcgtacctta gctgaaggcg
     9961 gagaacaaga catatcgatc cgtcagtatc gcgaccttta tgaacatgtg atacacacag
    10021 gattagtaga tatggtcgat atcgagttgt tcaaagtaga aagtctagga aaaagactga
    10081 tagaggaaat caagtcttta aacattcctt taattatcag tagtcatgat tttaaagaaa
    10141 caccagcaga ccctgtcttg ctttaccgat tgaatgttat ggaacatttt ggagccgata
    10201 tcggaaaact ggcagtaact cccaataatg aacgagatgt tttgcggttg atggagttga
    10261 ctagacgggc aaatgctttt gtctccatgc ctattattac gatgtccatg ggaaatcttg
    10321 gtaaaatttc tcgtctggtt ggcggaacga cgggttccgt tatgacattt ggttccttaa
    10381 gcagcgattc tacttctgct ccaggacaat taccggtcaa agcattgaag caaattattc
    10441 aattattaaa tgaaccgttg tagaacaagt ttgaggtgat attttttggc aaagaaaaaa
    10501 atagtaaaaa cgaatgcaat ccgtatggtc gaacaaaaaa agattcccta tacagagcac
    10561 gaatatgagt gggacgaaag ccacctaagt gcgtcatcgg tcgctgaaca gcttccagaa
    10621 agccaatctc gtatatttaa aacactggtt gctgtgggca atgtgacggg gcctcttgtt
    10681 gctgtgatac cgggagaagc cgaattgaat ctaaaaaaat tagcaaaagt ttcagggaat
    10741 aaaaaagtag aaatgctcca tttaaaagat ttggaagcaa cgaccggata catacgtggt
    10801 ggctgctcgc ctataggtat gaaaaaacta tttcctactt atttagatca aattgcagaa
    10861 agctatgaac aaatcattgt ctcggctggc agaagagggc tacagatgga gttagcacct
    10921 caagatatct gtgcattaac gagcggacaa tttgcagata taaaacaata aatcatacgt
    10981 ataaaagaaa gaaggaggcc gggatgttgt ttgtccagtc tccttctttc ttttttatac
    11041 attatgctct ttgcccggca ttcattcctg ctacatgccc tgtagcgaaa gccgctgtga
    11101 tattaaagcc gccagtataa ccattgacat ccagtaattc tcccgcaaaa tacagaccat
    11161 caatgatttt actctccaat gttttaggat aaatttcctt tagagaaacc cctccgccag
    11221 ttacaaatga tttctcaata ggaaaagttt tgttgattga tagcttaaac tctttgcata
    11281 gttgaacaaa atcttgaatt tgtttttgag aggcttgttg tcctgtcaga tgatccattt
    11341 caagccgctc aagaaagaaa actaacaatc gctcaggcaa tataccatgc caagcattga
    11401 ccaagttctt tttggttgct ttactttttt ctgtcaatat atgaataagc tcttgtttgg
    11461 tttgtgtagg gaaacaatcc agagaaagtg tcactggttc tcccgttttc tctaattctt
    11521 tattgacaaa acttgaacaa cgcagtgcag ccggaccaga aataccgaaa tgggtgaaaa
    11581 gtaaatccat cgtatgttca gtcaatacac gacctttttt atttaaaacg cgtaaagtga
    11641 tgtcttgtaa agaaagccct tgaagtgttt tctcctgaat atacggttca tccgatatca
    11701 agggggactc tgtcgcataa agaggcgtca ccgtgtgacc cactcgcttc acgatcttgt
    11761 acccatctcc agtagaacca gtagatggat aagtccgtcc accagttgtt aagataatgg
    11821 caggagcatg gaactcttcg aattccgtgc gtataccgta aattttatgt tctttgtgga
    11881 tcaatttctc tactcgagta gagaatagaa gcgtcacatc tagttctttc agacgattga
    11941 gcaatgcttc gataatcgtt tttgatttat ttgttacagg aaacatccgt ccatgatcct
    12001 cttcttttaa atgtacgcct tgtgattcaa agaagttcat gatatcgaaa ttattccatt
    12061 gcgcaaacgt actatataaa aactttccat ttccaggaat atgtgcaatc aaatcatcaa
    12121 caggacgatt gttcgtaaca ttgcaccgac caccacctgt catcagtagt ttttttcctg
    12181 cttttttgtt tttttcgatc aagagtacgc gtgcgccttg ttcagctgca ctgatcgctg
    12241 ccatcatccc gcttgttcca gcgccgacta cgatcacatc atacagtttt tccattccct
    12301 cacctcaagg aaagtgtagc atatctgaag aaagaaaaca gaaaaaaatg ttttgagcca
    12361 aaagagaaaa tgagctaaaa tgagggaaga taagcaaagg agcgtggaac gatgacaaac
    12421 gcaacagaat atgttcaagc aattcaagaa aagctacatc aaaaggatca agggcaagaa
    12481 gaatttttac aagcaatcga tgaattcatg cccacggtta tgtctttttt ggacactcat
    12541 cccgaatata tcgaaaagaa tatcttagaa cttttaacgg aaccagaaag agttattcaa
    12601 tttcgagtgc cttggcaaga tgatgaagga aactggcgag taaatagagg gtaccgtatc
    12661 caatacaact cagctatcgg cccttataaa ggtggattgc gcttccatcc tagtgtcaat
    12721 ctaagtatct tgaaattttt ggcttttgaa cagattttca agaatagttt aaccggtctg
    12781 ccaatcggtg gaggaaaagg cggaagtgac ttcgatccta aaggaaaatc tgataatgag
    12841 atcatgcgtt tttgtcaaag tttcatgctg gaattgtcaa aacatatcgg cccatcgatt
    12901 gatgttcctg cgggagatat cggtgtagga gctagagaaa tcggctattt gttcggcgaa
    12961 tataaacgat tgaaaaaata tgatactggt gtgttgacag gtaaaccttt ggatttttgg
    13021 ggaagtaaga tacgtacgga agctactggt tatggattag tgtattatgt caaacatcta
    13081 ttgaacgaag aaaaagattc atttaatcaa aaaacagttt ttgtttccgg aagcgggaat
    13141 gtagccattt atgcaatcga aaaagtgcaa gagctaggcg ggaaagttgt tacatgttca
    13201 gattctagcg ggtacatcta tgatccagaa ggaatagatg tgaaactatt gaaagaagtc
    13261 aaagaaatca atagacaacg tttgacggaa tacgcaaacc aacgtccttc tgctatttat
    13321 catgctggag aatctgtttg gacattgaaa gaaaaagcgg atatcgcttt gccatgtgct
    13381 actcaaaatg aaatagacga aagcttggcc gaaatcttag tagaaaatgg gatcaaaatc
    13441 gtagcagaag gcgctaatat gccatgtaca gtaaaagctg tttctgttct tcatgaagca
    13501 ggcgtgtggt attgtccagg aaaagctgct aatgctggag gagtagcagt atctgcttta
    13561 gaaatgagtc agaacgtcca gcgattgact tgggaaagag agcaagtgga tgagcagctg
    13621 gatcaaatca tggaaacgat ttaccatacg tgtcgccaaa cagcagccga gtacggcgac
    13681 gaaaaaaatc tgcttcttgg agcaaatgtt gctggatttg aaaaagttgc caaagcaatg
    13741 gcagcagaag gtttagtttg aaaaatgaaa ctttcttgga aatattgtca gcattttgtt
    13801 tatttgcttg aaaaataata aaaaaaaacg taaagtatta cctgtagagt aaacctataa
    13861 ggaggagaac aaatggcaca cattcatttt gactattcaa aagtagcacc gtttgtcaac
    13921 gaacaagagt tagggtacat gcaaagccaa gtaacagcag cccacaacga attacgggaa
    13981 ggtactggcg caggaagcga cttccgcggc tggatcgatt taccaacgaa ctatgacaaa
    14041 gaagaatttg cacgtatcaa agaagcagct aaaaaaatcc aatctgattc tgaagtatta
    14101 gttgttatcg gtatcggtgg ttcttattta ggcgctcgtg cagcgatcga cttcttgaat
    14161 cacacattct tcaatttatt agatagcaac acaagaaaag caccacaaat cttctttgcc
    14221 ggaaattcaa tcagctcaac atatattgct gacttgatcg aagtaatcgg tgatcgcgac
    14281 ttttcagtaa acgttatttc aaaatctgga acaactactg aaccagcgat tgcgttccgt
    14341 gtattcaaag agcttcttgt taagaaatat ggacaagaag aagcaaacaa acgtatctac
    14401 gctacaacag ataaagcaaa aggtgcagtt aaagtagaag cagatgcaga aggttgggaa
    14461 actttcgtga ttcctgatga catcggcgga cgtttctctg tattaactgc tgttggtttg
    14521 ttaccaatcg ctgttagtgg tgcagatatc gatgcattga tgcaaggtgc cgctgatgca
    14581 agcaaagcat actcaagcga caaactagaa gaaaacgaag cttaccaata cgcagcaatg
    14641 cgcaacattc tttaccgtaa aggcaaagta actgagttgt tgatcaacta tgaaccagga
    14701 atgcaatact tctcagaatg gtggaaacaa ttgtatggtg aatctgaagg aaaagaccaa
    14761 aaaggcatct atccttcaag cgcaaacttc tcaactgatt tgcactcttt aggacaattc
    14821 atccaagaag gacgtcgcaa tatttttgaa acagttgtga aggttgaaaa acctcgtaaa
    14881 tcaatcacaa ttcctgaaca agcagaagat ttagacggtc taggctactt gcaaggaaaa
    14941 gaagtggact tcgtaaatac aaaagcattt gaaggaacat tgcttgctca tacagacggt
    15001 gacgtgccta acttgctagt caaaattcca gaaatggatg cttatacatt gggttataca
    15061 atgtacttct ttgaaattgc tgtaggtatt tcaggttact tgaatggaat caacccattc
    15121 gaccaaccag gtgtagaagc ttacaagaaa aacatgttcg cacttctagg caaaccaggc
    15181 tttgaagatc ttgctaaaga attgaacgaa cgcctgtaag ataagggttt ttcgctagat
    15241 ttaaataatc agtgatagaa acatgttcgc tttaaaatga aaactggtac ccatttaggt
    15301 actcaaacat actgcatcgt ttattcagaa taaggggctg tgacaagacg tttgtcacag
    15361 tcccttgttt gtatagaaat cgattgattg ccttgctctt tagtttgttt taggacttta
    15421 agaacagtga ggctgacttc gaaggttaat tctgtttttt tgttttctag aatcagtaag
    15481 caaaaaagca ctcacttcga gtgctttttt gcgatatatt cttagcttaa gataggccta
    15541 atttttcttt tacagatgag ggtaccttat cgctggtaat tacttttttc tctatgatcc
    15601 gcttttcgct tgctttcaac tgcaagatcg ttccttcggg gtaagcagaa ccattggcag
    15661 agtcaccaga agtaatgaca tcgaaatcta actgtctttt ttcaccgttt tcagcataag
    15721 catctacgtt gtatttaaaa cctcttccaa ttacttcccc attatctgct ttgatatcag
    15781 tttctgcagg cattggagct ttgactaccg cataataatc tttacctaca taagtagact
    15841 gatagtagtc ccaacctttc catcctataa aagcaatcaa tatgataacg atcaacccta
    15901 aaattttttt catgaaagat ctcctttttt atttttatt
//
LOCUS       DAMYXM010000059        15360 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.060, whole genome shotgun sequence.
ACCESSION   DAMYXM010000059 DAMYXM010000000
VERSION     DAMYXM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.060"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(22..252)
                     /locus_tag="PNS21_002103"
     CDS             complement(22..252)
                     /locus_tag="PNS21_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159804.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(688..1254)
                     /locus_tag="PNS21_002104"
     CDS             complement(688..1254)
                     /locus_tag="PNS21_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDI5159805.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1254..2210)
                     /locus_tag="PNS21_002105"
     CDS             complement(1254..2210)
                     /locus_tag="PNS21_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDI5159806.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2210..2866)
                     /locus_tag="PNS21_002106"
     CDS             complement(2210..2866)
                     /locus_tag="PNS21_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDI5159807.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3236..5254
                     /locus_tag="PNS21_002107"
     CDS             3236..5254
                     /locus_tag="PNS21_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDI5159808.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5324..5530
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5681..8095
                     /locus_tag="PNS21_002108"
     CDS             5681..8095
                     /locus_tag="PNS21_002108"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDI5159809.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8443..9996
                     /locus_tag="PNS21_002109"
     CDS             8443..9996
                     /locus_tag="PNS21_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDI5159810.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10377..12161)
                     /locus_tag="PNS21_002110"
     CDS             complement(10377..12161)
                     /locus_tag="PNS21_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5159811.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12176..13897)
                     /locus_tag="PNS21_002111"
     CDS             complement(12176..13897)
                     /locus_tag="PNS21_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5159812.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13997..14758
                     /locus_tag="PNS21_002112"
     CDS             13997..14758
                     /locus_tag="PNS21_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDI5159813.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4999 a         2802 c         3007 g         4552 t
ORIGIN      
        1 tctcattaaa aagcataacc attactccga tctgttttta aaattagttt ctacctctaa
       61 tatatatcga gtttttattt tgccttcaga gaatactgtt tctttttttg cagttacagg
      121 ttgtttattt tcggaaaaag ctaatatagc taagattgaa acatccatct ggaatttatc
      181 ttttttactg ctttgatcat aaaagtctgc atattcatca ctgatatttt ttctaataaa
      241 ttcttccatc ataaaaatca cctcaaaacg attatacgaa cttacgttct tgttgtaaag
      301 cgatatttga ggggcaaaaa aggggcataa tttgactgat attaagttag ctattgatat
      361 atgaatgaca tacaaaaata taaaatcttt taaaaatgct tatatatcaa tgtttttgcc
      421 attcttttat atatctgtta tatgtcaaaa aaatgtgttc aatttcaatt tgtatttgaa
      481 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
      541 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
      601 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
      661 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
      721 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
      781 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
      841 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
      901 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
      961 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
     1021 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
     1081 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
     1141 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
     1201 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
     1261 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
     1321 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
     1381 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
     1441 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
     1501 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
     1561 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
     1621 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
     1681 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
     1741 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
     1801 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
     1861 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
     1921 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
     1981 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
     2041 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
     2101 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
     2161 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
     2221 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
     2281 acagccaaga aagactgagt aagtagccgc cgatgatatc agtaggaaaa tgaacgccca
     2341 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
     2401 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
     2461 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
     2521 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
     2581 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
     2641 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
     2701 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
     2761 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
     2821 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
     2881 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
     2941 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
     3001 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
     3061 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
     3121 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
     3181 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
     3241 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
     3301 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
     3361 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
     3421 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
     3481 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
     3541 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
     3601 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
     3661 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
     3721 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
     3781 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
     3841 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
     3901 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
     3961 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
     4021 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
     4081 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
     4141 ggaaaatggc tcttcgacga ataattccgg taacaatacc aactcttcag gaaatgctgg
     4201 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
     4261 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
     4321 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
     4381 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
     4441 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
     4501 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
     4561 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
     4621 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
     4681 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
     4741 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
     4801 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
     4861 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
     4921 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
     4981 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
     5041 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
     5101 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
     5161 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
     5221 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
     5281 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
     5341 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
     5401 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
     5461 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
     5521 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
     5581 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
     5641 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
     5701 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
     5761 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
     5821 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
     5881 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
     5941 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
     6001 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
     6061 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
     6121 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
     6181 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
     6241 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
     6301 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
     6361 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
     6421 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
     6481 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
     6541 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
     6601 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
     6661 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
     6721 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
     6781 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
     6841 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
     6901 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
     6961 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
     7021 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
     7081 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
     7141 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
     7201 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
     7261 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
     7321 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
     7381 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
     7441 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
     7501 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
     7561 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
     7621 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
     7681 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
     7741 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
     7801 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
     7861 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
     7921 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
     7981 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
     8041 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
     8101 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
     8161 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
     8221 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
     8281 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
     8341 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
     8401 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
     8461 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
     8521 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
     8581 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
     8641 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
     8701 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
     8761 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
     8821 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
     8881 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
     8941 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
     9001 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
     9061 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
     9121 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
     9181 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
     9241 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
     9301 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
     9361 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
     9421 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
     9481 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
     9541 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
     9601 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
     9661 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
     9721 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
     9781 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
     9841 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
     9901 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
     9961 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    10021 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    10081 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    10141 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    10201 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    10261 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    10321 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    10381 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    10441 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    10501 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    10561 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    10621 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    10681 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    10741 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    10801 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    10861 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    10921 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    10981 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    11041 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    11101 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    11161 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    11221 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    11281 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    11341 aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca
    11401 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    11461 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    11521 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    11581 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    11641 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    11701 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    11761 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    11821 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    11881 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    11941 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    12001 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    12061 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    12121 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    12181 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    12241 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    12301 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    12361 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    12421 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    12481 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    12541 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    12601 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    12661 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    12721 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    12781 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    12841 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    12901 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    12961 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    13021 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    13081 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    13141 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    13201 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    13261 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    13321 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    13381 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    13441 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    13501 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    13561 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    13621 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    13681 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    13741 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    13801 gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc
    13861 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    13921 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    13981 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    14041 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    14101 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    14161 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    14221 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    14281 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    14341 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    14401 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    14461 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    14521 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    14581 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    14641 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    14701 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    14761 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    14821 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    14881 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    14941 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    15001 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    15061 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    15121 attattacaa tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt
    15181 aatcaaatag tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt
    15241 tttgcttata aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag
    15301 aaaaatcaca aaggttattg ttatttttat atattctttt taagaatgat tgttttttta
//
LOCUS       DAMYXM010000060        15159 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.061, whole genome shotgun sequence.
ACCESSION   DAMYXM010000060 DAMYXM010000000
VERSION     DAMYXM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.061"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="PNS21_002113"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="PNS21_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDI5159814.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="PNS21_002114"
     CDS             complement(322..891)
                     /locus_tag="PNS21_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDI5159815.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="PNS21_002115"
     CDS             1077..1979
                     /locus_tag="PNS21_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI5159816.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="PNS21_002116"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="PNS21_002116"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDI5159817.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="PNS21_002117"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="PNS21_002117"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDI5159818.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="PNS21_002118"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="PNS21_002118"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDI5159819.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="PNS21_002119"
     CDS             8547..8732
                     /locus_tag="PNS21_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDI5159820.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="PNS21_002120"
     CDS             complement(8794..9990)
                     /locus_tag="PNS21_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDI5159821.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="PNS21_002121"
     CDS             complement(10160..11596)
                     /locus_tag="PNS21_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDI5159822.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="PNS21_002122"
     CDS             11889..12698
                     /locus_tag="PNS21_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDI5159823.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12884..15037)
                     /locus_tag="PNS21_002123"
     CDS             complement(12884..15037)
                     /locus_tag="PNS21_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDI5159824.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT         4436 a         3209 c         2676 g         4838 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc
    12781 agtttctcgg tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt
    12841 ttcttcatac tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca
    12901 tatcataata cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt
    12961 ctaataagtt cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg
    13021 ccacgatatt actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag
    13081 tccctgtttt tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag
    13141 gatcggtacc atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag
    13201 aaatattgac cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc
    13261 cattttcgtc attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat
    13321 tagcgactgt tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa
    13381 atgccaaatc ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact
    13441 tcttcatata atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag
    13501 tcgttgtacc aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt
    13561 cttcatctaa actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg
    13621 ccactttgat catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa
    13681 cttgtccgct tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct
    13741 catcaatcaa aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac
    13801 cagccttgac tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg
    13861 tgatttcttt ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct
    13921 gaggattcat agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca
    13981 atgtttgata attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag
    14041 ataacattaa attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc
    14101 cttcattatc aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt
    14161 gtttttccaa ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt
    14221 tctctgcttc atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt
    14281 tcagtgaact tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa
    14341 gttcagaagc gcgttctgcc acaacagcta actcttgatc agtaacacca gagtttttga
    14401 tatagacagt gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta
    14461 attgctcctc gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt
    14521 gttcgctgtt ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat
    14581 gtttgggatc agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg
    14641 gaacatcgat cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt
    14701 gtgttcctct cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg
    14761 tggagtcgta gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact
    14821 tcactcgcgc ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca
    14881 acgcaacaaa taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg
    14941 accggcgaaa cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaaccaag
    15001 aagtatttaa tatttttttc ataaaatctt tcatcatttc ctatccttcc gcctatttac
    15061 attcatgctt cattgtaccc aaaaacttct taataaaaaa gaaaaaatgg gcggagcaag
    15121 aaattcttta cctaatttca agataaaatc aactttttg
//
LOCUS       DAMYXM010000061        14721 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.062, whole genome shotgun sequence.
ACCESSION   DAMYXM010000061 DAMYXM010000000
VERSION     DAMYXM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.062"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            124..936
                     /locus_tag="PNS21_002124"
     CDS             124..936
                     /locus_tag="PNS21_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDI5159825.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            933..1733
                     /locus_tag="PNS21_002125"
     CDS             933..1733
                     /locus_tag="PNS21_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDI5159826.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1894..3162
                     /gene="rseP"
                     /locus_tag="PNS21_002126"
     CDS             1894..3162
                     /gene="rseP"
                     /locus_tag="PNS21_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDI5159827.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3230..4939
                     /locus_tag="PNS21_002127"
     CDS             3230..4939
                     /locus_tag="PNS21_002127"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDI5159828.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5151..9503
                     /locus_tag="PNS21_002128"
     CDS             5151..9503
                     /locus_tag="PNS21_002128"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDI5159829.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9647..10120
                     /gene="rimP"
                     /locus_tag="PNS21_002129"
     CDS             9647..10120
                     /gene="rimP"
                     /locus_tag="PNS21_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDI5159830.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10144..11319
                     /gene="nusA"
                     /locus_tag="PNS21_002130"
     CDS             10144..11319
                     /gene="nusA"
                     /locus_tag="PNS21_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDI5159831.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11341..11634
                     /locus_tag="PNS21_002131"
     CDS             11341..11634
                     /locus_tag="PNS21_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDI5159832.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11631..11942
                     /locus_tag="PNS21_002132"
     CDS             11631..11942
                     /locus_tag="PNS21_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HDI5159833.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11955..14261
                     /gene="infB"
                     /locus_tag="PNS21_002133"
     CDS             11955..14261
                     /gene="infB"
                     /locus_tag="PNS21_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDI5159834.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14288..14635
                     /gene="rbfA"
                     /locus_tag="PNS21_002134"
     CDS             14288..14635
                     /gene="rbfA"
                     /locus_tag="PNS21_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDI5159835.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         5032 a         2588 c         3276 g         3825 t
ORIGIN      
        1 attaaaaaaa gaaaaaatta ttcagttttt aaataaaaaa acaatgattt cataggaaaa
       61 acagttagat attgctataa tacttaagat agtgggaatc ttttaagtat aggaggaaaa
      121 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
      181 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      241 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      301 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      361 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      421 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      481 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      541 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      601 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      661 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      721 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      781 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      841 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      901 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      961 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
     1021 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
     1081 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1141 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1201 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1261 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1321 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1381 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1441 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1501 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1561 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1621 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1681 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1741 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1801 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1861 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1921 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1981 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     2041 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     2101 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2161 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2221 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2281 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2341 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2401 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2461 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2521 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2581 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2641 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2701 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2761 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2821 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2881 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2941 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     3001 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     3061 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3121 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3181 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3241 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3301 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3361 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3421 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3481 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3541 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3601 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3661 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3721 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3781 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3841 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3901 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3961 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     4021 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     4081 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4141 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4201 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4261 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4321 attccactat aaaaatgtga atccaaaacg cgatttcact gtgctttctt atgaagatct
     4381 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4441 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4501 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4561 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4621 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4681 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4741 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4801 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4861 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4921 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4981 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     5041 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     5101 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5161 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5221 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5281 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5341 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5401 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5461 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5521 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5581 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5641 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5701 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5761 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5821 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5881 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5941 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     6001 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     6061 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6121 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6181 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6241 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6301 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6361 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6421 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6481 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6541 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6601 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6661 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6721 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6781 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6841 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6901 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6961 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     7021 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     7081 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7141 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7201 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7261 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7321 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7381 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7441 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7501 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7561 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7621 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7681 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7741 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7801 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7861 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7921 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7981 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     8041 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     8101 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8161 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8221 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8281 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8341 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8401 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8461 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8521 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8581 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8641 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8701 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8761 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8821 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8881 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8941 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     9001 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     9061 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9121 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9181 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9241 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9301 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9361 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9421 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9481 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9541 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9601 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9661 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9721 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9781 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9841 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9901 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9961 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
    10021 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
    10081 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10141 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10201 aaagaaatcg tgatcgatgc tttagaagca gcattggttt ctgcatacaa acgtcactat
    10261 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10321 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10381 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10441 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10501 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10561 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10621 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10681 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10741 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10801 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10861 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10921 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10981 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    11041 acaaatccaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    11101 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11161 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11221 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11281 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11341 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11401 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11461 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11521 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11581 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11641 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11701 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11761 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11821 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11881 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11941 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    12001 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    12061 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12121 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12181 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12241 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12301 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12361 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12421 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12481 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12541 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12601 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12661 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12721 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12781 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12841 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12901 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12961 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    13021 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    13081 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13141 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13201 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13261 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13321 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13381 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13441 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13501 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13561 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13621 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13681 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13741 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13801 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13861 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13921 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13981 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    14041 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    14101 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14161 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14221 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14281 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14341 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14401 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14461 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14521 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14581 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14641 aaatcccgca aatcctgtga aatcaggggt tgcgggattt tttggctctt tgtcaataag
    14701 gactgatggg ttgtacaaaa t
//
LOCUS       DAMYXM010000062        14351 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.063, whole genome shotgun sequence.
ACCESSION   DAMYXM010000062 DAMYXM010000000
VERSION     DAMYXM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.063"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..135
                     /locus_tag="PNS21_002135"
     CDS             <1..135
                     /locus_tag="PNS21_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDI5159836.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="PNS21_002136"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="PNS21_002136"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDI5159837.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2411..2662)
                     /locus_tag="PNS21_002137"
     CDS             complement(2411..2662)
                     /locus_tag="PNS21_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HDI5159838.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2808..3431
                     /gene="lexA"
                     /locus_tag="PNS21_002138"
     CDS             2808..3431
                     /gene="lexA"
                     /locus_tag="PNS21_002138"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDI5159839.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3611..3829
                     /locus_tag="PNS21_002139"
     CDS             3611..3829
                     /locus_tag="PNS21_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159840.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4118..4630)
                     /locus_tag="PNS21_002140"
     CDS             complement(4118..4630)
                     /locus_tag="PNS21_002140"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDI5159841.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4644..6941)
                     /gene="recJ"
                     /locus_tag="PNS21_002141"
     CDS             complement(4644..6941)
                     /gene="recJ"
                     /locus_tag="PNS21_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDI5159842.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7083..7526)
                     /locus_tag="PNS21_002142"
     CDS             complement(7083..7526)
                     /locus_tag="PNS21_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDI5159843.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7527..8315)
                     /locus_tag="PNS21_002143"
     CDS             complement(7527..8315)
                     /locus_tag="PNS21_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI5159844.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8312..9253)
                     /locus_tag="PNS21_002144"
     CDS             complement(8312..9253)
                     /locus_tag="PNS21_002144"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDI5159845.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9430..10017)
                     /locus_tag="PNS21_002145"
     CDS             complement(9430..10017)
                     /locus_tag="PNS21_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HDI5159846.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10238..11545)
                     /gene="obgE"
                     /locus_tag="PNS21_002146"
     CDS             complement(10238..11545)
                     /gene="obgE"
                     /locus_tag="PNS21_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDI5159847.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11839..12849
                     /gene="gap"
                     /locus_tag="PNS21_002147"
     CDS             11839..12849
                     /gene="gap"
                     /locus_tag="PNS21_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDI5159848.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12934..13869)
                     /locus_tag="PNS21_002148"
     CDS             complement(12934..13869)
                     /locus_tag="PNS21_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI5159849.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            14055..>14351
                     /locus_tag="PNS21_002149"
     CDS             14055..>14351
                     /locus_tag="PNS21_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5159850.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKER"
BASE COUNT         4020 a         3190 c         2528 g         4613 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 tacctttatt tcttacactt tcattctata agatgtcaca cgaaaaagca atctttttca
     2401 gtatttttta ctaaaagttg tcatttcggt tgtgtaagcc ttttttcttt tggatttctt
     2461 ttaatttttc cggggtcacg tcattgccct caggatcgac gactttcatt ccttcgatat
     2521 gatgacgcat accggaacga aaagcttcca aatactcttt gcgcaagttg atttgttctt
     2581 gttcttcggc ttttgagaga ccattttcct tctttttacg tgcaagttca ttgatccgct
     2641 ctattttttc aggtgacagc atgctcagcc ctccttttac tctccttcat attatataaa
     2701 aactgaaaaa aaacaactag ataaactaaa taaaatacga acattcgttt gatttagcgc
     2761 tttttatatg gtactattta tttatcaagt gaaagaaggt gtaccttgtg gcccgacaaa
     2821 ctgaatcaag acaaatcgaa gtcttacgat atatccatga acaagtaacc caaaaaggat
     2881 acccgccgac tgtccgtgaa atcggggaag ctgttcagct ttcttctact tctactgttc
     2941 acggtcatct tgcacggctt gaaaaaaaag ggtttatcca aagagatccg acgaaacctc
     3001 gtgcaatcga gttgacaaaa caaggtttag aaaagattgg gattcgtcca acaactattc
     3061 cagtacttgg cgtcgtaaca gctggagaac ccattttagc agtagaggat gcttcagact
     3121 tctttccaat ccctcctaat ctttcttccg aagaaggcag tctctttatg ctgacaattc
     3181 gtggagatag tatgatcaat gctgggatat tggatggtga caacgtgatc gttcggaagc
     3241 aagaaactgc tcaaaatggc gatattgtca ttgcaatgac tgccgaggac gaagcgactt
     3301 gcaaacgatt ctataaagaa aaaaactttt tccgtctgca gcctgaaaac gacttgctcg
     3361 aaccaatcat cttagatcaa gtatctatat taggacgagt agtaggactt tatcgtagtc
     3421 atatctatta attgctacta attaaaagaa aactttttaa aaagagtttg agacgttggt
     3481 ctcaaactct tttttcgccc gggttcttct tctttcaaat ttttatcact tccctcttta
     3541 caaaccgaac aaatattcgt atactaaata tacgaacaat tgttcgcata aattaaagag
     3601 gtgagaattc atgaaatcaa tgagacgtct tggcttagta ttaactgttt tgtttttagg
     3661 tattattatt ggtaatttcg gcatacgtcc agctttactt gtgtttgttc cgttatttac
     3721 tttgtggttc atgttgtggg atgaaaagaa gtaccgtcaa tctgagcggc gcagaattca
     3781 tgaagatcat ttctatcatg agccctacta cagatccagg caatcataat aaggattgac
     3841 tgctaattat tcaaaagaca agaagttaaa cttttacggg ggtgtgacaa aaatcggtca
     3901 catccttttt ttcgaataaa cggtgttcta aagccagttt ttcggtatta agtcaaattg
     3961 cggaaaaagt gattttttaa acttctactg gtctttgctt gtttccattt ataaaaaaat
     4021 agcgaccctc aaaagtcaga tagagaatct gacttttgag ggtcgcttta aaatcgcttc
     4081 tttaagaata acatcaagaa tagcttcttt tttctgctta atattccatt aatgtaatga
     4141 tgtcatatcc tttgattttg tcgcgtccat ggagatccat caattcgatc aaaaatgcgc
     4201 agcctacgac tacaccgcct aattgttcga tcagttcgat cgtcgctttg attgttcctc
     4261 cagtagctag caaatcgtca caaatcagca cacgttgacc tggtttaatg gcatccttat
     4321 gcaatgtcaa tgtatctgtt ccatattcta atccataagt cacttcgatg gtttctcgag
     4381 gtaacttccc ttttttgcgg actggggcaa aaccgactcc taactcataa gcaacaggac
     4441 atcctacgat gaatccacgg gcttctggcc caactaccat atcaattcgt ttttcttttg
     4501 cataatttac gatttgttta gtagcttccc gataagcgtc accatctgcc atcagtgggg
     4561 aaatatctcg aaacacaatt ccttttgacg gatagtcggg aatactggca atataatctt
     4621 ttaaatccac tctttgtttc ctcctaggtt gttaaccatt tctttatggt aggaatatcg
     4681 ctcaaaagca aaaattcctc aactttgatt ttattgagcc gtcgctggta taatctgctc
     4741 tcagtcaaag gatgactttc tgggctagcg actttttgta aaacaccgtt ctctattgta
     4801 acaaatttca attcaaaaaa cacctgtatc ataaaaatta ataatttttc agggatacgc
     4861 aaatattgag ctattgcttt tattttatga cgtatatcaa ttcgttcttg agaatggatc
     4921 aatttaaaca attttgcgta ctgttctctt gtgcctaccc cattcaaata ggcctcatca
     4981 ggtgaaatcc ccatcagata aacacgattg aatgaataag accgaaaaac ttctttcaga
     5041 tccgctaaat catctggaca atccataaca actagagagt ttggttttct tttttcaatt
     5101 attgccttta cagaatctag tccttcgaaa acaatcgcag cttcctggat aatctcaggc
     5161 acgaatttca acgtcgaaga atcaaatgct aaataaaggc taccttccga cgttcggctc
     5221 tgttcacgga aacgttttgc ccgccaatca aatacttgca gaccacccac agaaaaatca
     5281 gataccatca gctgcggttt cttacgcccg ttccattcat tgatcgaaag ctttcctacc
     5341 acatctacct gattattcag cagttcttct tcttgagtac caaaaccaaa agcgaccgca
     5401 tcaagctgac tcgatgcatc ggaaaggaca aatttcagat gctgttggtt tgctccgatt
     5461 cttttgatat tttctgcttg gacttgtcga aataaaaact gaggcaaagg attatccgta
     5521 ccaaatggcg ccaatagttt cagctcatca attcgctcta ctgttacttc atttggcagc
     5581 aatacttcat caattctcaa ctcaggccct ctcatcaagt caatttgatg atcgactata
     5641 tattggttca tcttctcctg tagtatagtg acattttcac ttggcatcgt taaaccgact
     5701 gctgcatgat gtcctccgaa gtaagtaaat aaatctcgca tctggtcaag catttcaaaa
     5761 agattcaagg cttcaatact acgtccagat cctttagctg atccatcttc ttttaaagta
     5821 aggacaagcg ttggtttacc cgtttcattc attattttcc cagcaacaat gcccaaaacg
     5881 ccttcatgcc atcctcggtt taccaataga tggatatgat tctccttatt gaccatcgct
     5941 aatgcttctt gtgttatctg ctctacgatt gcttttcttt cttcattgat ctcattcaat
     6001 ttttttgctt gaacggatgc ttcctcttca tcaaacgtcg caagtaacga gacggcagga
     6061 cgtggatcgc ccatccgacc gattgcattt agtctgggag caatcgaaaa accgatcgtt
     6121 gtttcgtctg cgtccctcat ttttacgccg ctttcttcaa atagcgcctg aagtcctatc
     6181 cgttctgaat gatgaatggc atcgattcct aatgaaacaa ggattcgatt ctcatcagtc
     6241 aatgaaacta aatcggcgat cgttcctatc gctactaaat ccaaaaattc tgcaggcggt
     6301 tcttccaata atgcagaagc tactttgaat gctactccga ctcctgccaa atctggaaat
     6361 ggatagtgcc ccgctggatg acgagggtga acaatcgcat aagcgtctgg taaattctca
     6421 ggtagttcgt gatgatctgt aatgatcacg tctacaccag cttgctgagc gtatgctacc
     6481 gcatcatttc ctgctacccc gttatcaaca gtgatgatca actggattcc ttcttcgatc
     6541 ttttcttggt ataccgattg gtttggtcca tacccatgct caaatcgatt tggcaggaat
     6601 acttctactt ctgcaccaag taattccagt gtttctttca ttaccgttgc actggtgatc
     6661 ccatctgcgt cataatcacc gtaaacgagg attttttctc cactgattac agcttcttgg
     6721 attcggctga ttgctttatc catatcgtgt atcagatacg gatcatgtaa ttcttgttcc
     6781 tttggatgta aaaatcggtt gatatccttt tcctcatggt aacctctttg ccacagtaac
     6841 ttaccgacaa atggagataa tttcagttca cggatcattt ggagaaagga ttcagaaggc
     6901 tctgtctcgc ttaacttcca atcaaaattt gcttgtctca ctttctcact cctaacttag
     6961 ttattatagc aaaggaaatc agcaaaaaaa agcagaaaga aagaataaaa acagatcaga
     7021 tccgctagac agatgttcag caggacctga tcttttgtac gaccatttat gaaatatccc
     7081 tattagtcaa aataattgaa gctttgtttg ggcgattgag ttttttgttc ttccggcgct
     7141 ttcaattgac tgatttgtgc ggttttcgct tgtaactccg cctgataagc agcttccaac
     7201 tccgcacgtt cgtttctaaa ctcgcgttgc tgttcttctt ttgcttcttc aattttcttt
     7261 tgaagttctg tttgatattc tgacatttcc tgagtcagct gtttgatttg ctttttttgc
     7321 tgccatagtg ttgccgaagc agtcaaaaat acaatcagtg caccaatcag cgcagaaccg
     7381 atgatcacta agatcaatgg cgcagaaaag gaagtgaagc caaaacttac aggaacattt
     7441 ttattgttca atacagcaaa aagaacaata atcaaaacta aaacaaatcc aagaatcaca
     7501 ttgctttgct ttttcaattc cttcacctac tttttgttaa aaattccgct ggctaaataa
     7561 tcaccaatcg ttggaaacag cgtataaaat tttgatgcaa cttctaaaat aaacggttga
     7621 ttgatttccc gttttggatg tctcatattg cggacgatca tttttgccaa cttggcaggt
     7681 tctaaaacga tttgtccgac tttttccaaa tagcttccgc ttggatcagc cttatcgaag
     7741 aaatttgtct cgattggacc tggattcact gtcgtaacag ctacgccaag tggtttcaat
     7801 tctaatcgca aagcgttgga gaagccaagg actgcaaatt tcgtggcaga ataaaccgtt
     7861 gattttgcag ttgccatttt tccagccata gaggcgatat taatgatatg accttgtctt
     7921 ctttctgcca tatcgatcgc aaatttttgt gtcaaaacca tcattcctaa gatgttgact
     7981 tcaaacatat cgtatgcttt tccaaggtcg aatgtaagga agtcttcgaa aatcccaaaa
     8041 cccgcattgt ttaccagaat atcgacgcgc ccgacttctt ccatcacctt ttcatataat
     8101 tcgtccacgc tttcaggatt gctgacgtct accttgaaag cataagcctc tttgccgctc
     8161 aagcgtctgc actcgtctct gacaccttct acaaatgcca ttcttcgtgc gcaacttata
     8221 acgattgctc cttgctttgc tgcttcataa cagatttgtg cgcccagacc gcctgaacta
     8281 cctgttacta aaacaacttt atcttctaaa ttcatgcctc ttcctcctcg cgaaatggaa
     8341 tctcaatgct atcaaaatct ttcataatcg tcgtgttttc aaacacttct tgtgcttctt
     8401 tttcaagctc taaagctgct tttcctaaat atctcgcact gatatgagta agtagcagcc
     8461 gttttgcctg cgcttctttt gcaacttcag cagcttgatg agtcgtggaa tggaaatagt
     8521 tgtgcgccat tcttgcttca tctttattaa atgtgctttc atgtaccaga atatcgctat
     8581 tttccgcaag cacaacggaa ttatgtgttt ttctcgtatc gcctaaaata gtcacgattc
     8641 gtcctttttt atctggacct acgaaatctt ttccattgat cgtccgccca tcttctagtt
     8701 ggatcgtttc accttgcttc agtttgccgt acagtggtcc agctggaata cctaattctt
     8761 ttagacgatc cacctgcaat tctcctttat gatcatgttc aaccacacga taaccaaaac
     8821 tgtcgatccc atgatttagt ttttttgtat aaacagaaaa ttgctgatca gtaaaaatcg
     8881 gggctgtttc atttagctca atgaatttca gcggataact taaacgcgtt tgcgaaatac
     8941 ctaaagagac ttttatgtat tcctctatcc cttttggccc gtaaatctct aatggcgtat
     9001 ccccaccttg aaatgaccgg ctgctgatga gcccgggaag tccaaaaata tgatcgccgt
     9061 gtaaatgtgt aatgaaaatt ttaccgattt ttctcggacg aatagtcgtt cgcaaaatct
     9121 gcatttgcgt gccttcgcca cagtcgaaca accaaacttc attgcgctcg tctaaaagtt
     9181 tcaatgctat acttgtcaca tttctatgtt ttgcaggaac acctgcacct gttcctaaaa
     9241 attgtaattc cataatctac ctgactttct ttaagttcct ctttaatttt agggtaaaaa
     9301 gaagggataa tcaaccgtaa gtcgagaatg ggttcaaatt aaaaataacg ttctaaagcg
     9361 atagcgtttt ctttgaaaat tactataaaa acagcagcga acccgtttga gatcctctgc
     9421 tgttttttat taatttgaag atccagaact ttcattgtcg ttggaagttt gcggatgttt
     9481 gtccttccaa gtcttttgtg cagcttcttt cgcatgattt tcagcttctt tcgcatcatg
     9541 cacatctttt tcgacatctt cataatccaa gcgagtaatt tctccgccta attccatcat
     9601 taccaattga ttcagtgggc gattgtcctc ttcatcagca atcaagatca gtcctgtagc
     9661 tccttcaccg attttatttc ctacaaagtc gaacacggtc tgggcatctt ttatttcttt
     9721 ggcatcttgt gtcgcaccga tcattcctcc ggcaaaccat cctaataaaa tccctagcgg
     9781 accacctaat acaccaacca acattccgat taatccaccc gtagaagatt tgttattacc
     9841 ggtaaagtcg ataaaatctt ctattttgaa ttgatgtgtc ccattaggat catggctgac
     9901 aactgccatc tgttctcctt taatcgaacg aacagcatgt aactttttga tttctgaaaa
     9961 tgcctgatat gcctggcttt ctacttgaaa atttaaaatg attactcgtt tgctcattaa
    10021 aaacgcccct ttcatacctt cttgttattt attttacctt taattgaaaa ataagaaaag
    10081 aaaagcactc tttttttcca ataaaatagg gctgtgacaa aagtcgtatc acagctcctt
    10141 tttccgaata aacggcgttc ttgaagctga tcacttctgt cacaacctcc cttaaatctg
    10201 aatctatatt ttttggtatt tgtttttatt tgcttgccta ttcgacaaat tcaaattcaa
    10261 actcaccgat acgtacaagg tcgccatctt tcgcaccgcg tgcccgaaga gcttcatcca
    10321 cacccatacc acgtaattga cgagcaaaac gcatcactgt ttcatcatga tcgaaattcg
    10381 tcatttggaa taatttttcg atcttctcac cagataagat ccacgttgca tctgaatcac
    10441 gatcgatttg gaactcagga ccttctggat tgaatccata gtgtacagtt tcttcttcca
    10501 gttcttcttc gtacaatggg aattctggcg ttacttctaa caaatctgct gtagcattca
    10561 ataacgcgtc caatccttgt cgagtcactc cagaaattgg aaatactggg atgtcatcag
    10621 caaattcatc ttctttttcc ttgttcagct gttctttgaa tttcactaga ttttccggtg
    10681 catccggcat atccattttg tttgccacga tgatttgcgg acgctctagc aagcgtaagt
    10741 tataagtaga taattctttg ttgatggcaa gatagtcttc ataaggatct cgtccttcca
    10801 tcccactcat atcgatcaca tgtaaaatta ctcgtgttct ttcgatatga cgaaggaatt
    10861 gtgtacccag cccaacacct tgcgaagctc cttcaatcaa gcctggcaag tctgctgcgg
    10921 caaaacttct gccatcactt gtagtaacca ttcctagatt aggtacgagc gtcgtgaaat
    10981 gataagcgcc gatttttgga cgagcagatg aaataacgga tagtaaagtc gatttcccga
    11041 cagatggaaa acctacaagt ccaacgtctg ccaatacttt caattctagt tcgatttttc
    11101 gctcttgtcc tggttcaccg ttttcagcaa tttccggtgc aggattccga ggagaggcaa
    11161 aacggatatt ccctcgtcca ccgcgtccac cttttgccac aactagtgtt tgaccatttt
    11221 caattaaatc tcctaaaaga gcaccagtct ccgcatctcg gacagtggtt ccttgtggta
    11281 ctttaacata ggtatcttct gaaccacgac cgtgcatccc tttactcatc ccattttcac
    11341 ctggctgcgc tttgaaatgg cgattgaacc gaaaatccat caatgtacgt aacccttcat
    11401 cgacgatcaa gatgacatcg ccgccacgac caccgtctcc gccagccggt ccgccgtcag
    11461 gaacgtattt ttcgcgacga aatgcaacca ttccgtctcc gcccttgcca gctttcacat
    11521 cgatcgttac ttgatctaaa aacatggaca taattaagtc ctcctattat tcatttattt
    11581 atttgactgc ctgaatttcc acaataaaca aaagtcttac ttattttatc ggtgaaatct
    11641 gattttgtct atcatttcag caaaaagatt gtttaccttc ttcttttttt atcatttggt
    11701 aaggtttcca accttttttc tgctaccaag tataattttt tgacaaaata ttctttgcgt
    11761 agcagaataa cagtgtatta attgtattaa ttgaggatgc cgtttcacag cacccctaat
    11821 aaaggaggaa cttttgatat gacagtaaaa gtaggtatta acggatttgg gcgtattggc
    11881 cgtctggctt tccgccgcat caaagaagtc tcagatgata ttgaagtagt agcaatcaac
    11941 gacttaacaa gtccaactat gttagcacaa ttattgcaat ttgactctac tcatggtact
    12001 tatcctggca cagttactgc aacagaagac tcaatcgtag tggatggcga agcaactcgt
    12061 gtttatgcag aacccgacgc aagcaaaatc ccttgggcaa aagaaaacgg tgtagatatc
    12121 gtactagaat gtactggttt ttatacatct gaagaaaaag caaaagcgca tttagatgct
    12181 ggtgtcaaac gagtagttat ttctgcccca gctggtgcta tgaaaaccat cgtctataac
    12241 gtcaatgatg atacattaga tgaaaacgac aaaatcatct cagcaggttc atgtacaaca
    12301 aactgtttag cacctatggc ttatttctta aataatgaat ttgggatcga agttggtaca
    12361 atgacaacag ttcatgccta cacttccaca caaatgctct tagatggacc tgtaaaaggc
    12421 ggtaatttgc gtgctgctcg ttcagctgct gacaatacta tcccacattc tactggtgct
    12481 gcgaaagcaa tcggcttagt tatccctgag ctacaaggaa aattacaagg acacgcacaa
    12541 cgtgttccag tcgttgatgg ttcattaact gaattggttt cagtattgaa aacaaaagta
    12601 actgctgatc aagtaaacga agcaatcaaa aaacacacag ttgacaatcc ttcattcggg
    12661 tatgatgacc gtcaaatcgt ttctagcgat gtcattggaa caactcaagg ttcgatcttt
    12721 gacccaacgc aaacagaagt aacaactgct ggtgatttcc aacttgttaa aactgttgca
    12781 tggtatgaca atgagtatgg atttacttgc caaatgattc gcttactaga aaaattcgct
    12841 aacttgtaat ttgatagatc aatcgataaa agaagccttt gactggatcc agtcaaaggc
    12901 ttctttcttt tcatccccta cttttaaact cgttcaatga acatattggc ttaaaaattg
    12961 ttggactact tgctgatatt tttcaggatc tgtcttgtat gcttccgcat gctcggctcc
    13021 ttttacaaca tacttttctt ttggtccctt cgttgcacga tagaccttat ctaacatttc
    13081 aaatggtaca aatgtatctt cgtctccatg tataaagagc attgggcgct ggtttttctt
    13141 caattgttcc actgcatttg cttcaccgaa aaagtagcca gcacggactt ttgtcactag
    13201 actcgttact tgcataagcg gaaatgctgg aagactaaac atatcgtcta gctgataagc
    13261 aagctcatca tgaacggaag aataaccgca atcttcaatg attgctttca cattatctgg
    13321 tagtttttct ccgctagtca tcataactgt tgctgctccc atgctgacac cgtatagaac
    13381 gatttcttgc gattttccat tgttttctag cactttgttt atccactgta catagtcttt
    13441 acgctctggc catccaaatc caatgtaatc cccttcgctt tttccatgtc ctcttgcatc
    13501 aggaacaagg acattgtaac ccatatcatg aaacatcttt gcgtaattgg tcatcgtttc
    13561 agcgtttccc atatagccat gagccacaag agctgttttt tcacttttcg tttcagcagg
    13621 aagataaatc gctgataatt tcagaccgtc ttttgaagtg atggtccaat cgcttcggtt
    13681 ttcttttgaa ctaaaccatt tatgtgcatc cgtttctttg gatgtctgtg ttgtaccagg
    13741 tgtatcttcg tctaaaaagt ctttctcaga aggcacaaca gcataattat aaaaataatt
    13801 ccctgcaaac actaacgcaa caatcagagc taaagcgacg cctaaaagga ttttcatcca
    13861 aagtttcata aatcatccat ctcatttctc ttttttcata taaccttgaa ttagtataat
    13921 caaagaatct ctactatgca atgttcattt ttataacccg cgaattgtaa aattaagtta
    13981 gaaaaataaa aaggcattta tggtacactc aaattgtatt tccgacgaaa gaaaacaaag
    14041 gagtgtaacc ataaatgacc tacaaacatc ttaccataga cgaactgaca atgatagaat
    14101 catattatct tcaacataat aagccggttg aaatcgctaa ccgaatggga cgtgctatac
    14161 aaactattta taatgtagtc aataagttca agcaaggcaa gactgctctt gattattggc
    14221 accagtataa agaaaataag aaaaaatgtg gtagaaaagt cattcaatta cctgctcatg
    14281 aagtagatta cattaaagag aaagtcactc ttggttggac gcctgacgtc attatcgggc
    14341 gaaaagaaag g
//
LOCUS       DAMYXM010000063        14108 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.064, whole genome shotgun sequence.
ACCESSION   DAMYXM010000063 DAMYXM010000000
VERSION     DAMYXM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.064"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..387
                     /locus_tag="PNS21_002150"
     CDS             <1..387
                     /locus_tag="PNS21_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDI5159851.1"
                     /translation="RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGK
                     CAITLEEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIAL
                     LKLNKVQLIRLNKYRVLAGNQELNLI"
     gene            491..715
                     /locus_tag="PNS21_002151"
     CDS             491..715
                     /locus_tag="PNS21_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159852.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            832..1329
                     /locus_tag="PNS21_002152"
     CDS             832..1329
                     /locus_tag="PNS21_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDI5159853.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            1304..1810
                     /locus_tag="PNS21_002153"
     CDS             1304..1810
                     /locus_tag="PNS21_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDI5159854.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            1794..3299
                     /locus_tag="PNS21_002154"
                     /pseudo
     CDS             1794..3299
                     /locus_tag="PNS21_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            4044..5870
                     /gene="ltrA"
                     /locus_tag="PNS21_002155"
     CDS             4044..5870
                     /gene="ltrA"
                     /locus_tag="PNS21_002155"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDI5159855.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            5905..6921
                     /locus_tag="PNS21_002156"
                     /pseudo
     CDS             5905..6921
                     /locus_tag="PNS21_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6924..9101
                     /locus_tag="PNS21_002157"
     CDS             6924..9101
                     /locus_tag="PNS21_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDI5159856.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            9098..10099
                     /locus_tag="PNS21_002158"
     CDS             9098..10099
                     /locus_tag="PNS21_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDI5159857.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            10096..11028
                     /locus_tag="PNS21_002159"
     CDS             10096..11028
                     /locus_tag="PNS21_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDI5159858.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            11273..11389
                     /locus_tag="PNS21_002160"
     CDS             11273..11389
                     /locus_tag="PNS21_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDI5159859.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            11405..13324
                     /gene="tet(M)"
                     /locus_tag="PNS21_002161"
     CDS             11405..13324
                     /gene="tet(M)"
                     /locus_tag="PNS21_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HDI5159860.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            13443..13610
                     /locus_tag="PNS21_002162"
     CDS             13443..13610
                     /locus_tag="PNS21_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDI5159861.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(13670..14023)
                     /locus_tag="PNS21_002163"
     CDS             complement(13670..14023)
                     /locus_tag="PNS21_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5159862.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSIM
                     ESLASGINEARNIED"
BASE COUNT         4637 a         2532 c         2954 g         3985 t
ORIGIN      
        1 agaaaattta tccacaccaa tctaagtgtt gataaattac tgatgtacca gttaatgaaa
       61 ttatctttgg aaaatcgcac aatagaatac gcagacaatc gaatttcgct attttctgct
      121 caacatggaa aatgtgctat aactctggaa gaatttcaac aagctaatga aatacattgt
      181 catcatattg taccaactgg agctggtgga aatgacgatt ataaaaatct tattttggta
      241 aaagaggcgg tacaccgtct aatacacgct aaaacagaag aaacaataca aaaatatata
      301 gctctattga agctaaataa agtccagctc ataagactaa ataaatatag agtattagct
      361 ggcaatcaag aattaaactt gatataggtc tactacataa atacttttca atataaacac
      421 gatggaacgc ggagtgcggt gaaagtcgca cgctccgtgt gaatgggggg aaaagtttga
      481 gatatttata atatcaggaa cttacctatc catatataac tggtttctat caaacgctca
      541 atcactggtg cttttagcaa tcgttgtgat tggcttgtat cttggcttca agcgtgagtt
      601 tagcaaactg attggctttt taattattgc gattattgcg gttggcttag tcttcaacgc
      661 tgctggagta aaagacattt tactagagct attcaatcgc attattggtg cttaaataaa
      721 accgttcttt tgtggaatat aagtggtttt cttatgttcc gcaaaggaat ggtacaccaa
      781 acgaagtgcg gtagggattt ttgaatctct acaaagaaag gacgtgaata tatggacgat
      841 atgcaagtct atattgcgaa tttaggcaaa tacaatgagg gcgaattggt cggtgcgtgg
      901 tttacctttc ccattgactt tgaggaagtc aaagagaaaa tcggcttgaa tgatgaatat
      961 gaggaatacg ccattcatga ctacgagtta ccctttacgg ttgacgaata cacttccatt
     1021 ggcgaactca atcgactatg ggaaatggta tcggaattac ccgaagaatt acaatcggag
     1081 ctatctgctc tgctcactca tttttcaagc attgaagaac taagcgaaca tcaagaggat
     1141 attatcattc attccgattg tgatgatatg tatgacgtgg cacgctacta cattgaagaa
     1201 acgggtgctt taggcgaagt accagctagt cttcaaaact atattgatta tcaagcctat
     1261 ggtcgggatt tagacctttc aggaacgttt atctcaacca atcatgggat ttttgaaatc
     1321 gtctattaaa tctgtcggta cattactact ggcagatttt ctattttacg gggtggctca
     1381 atcagctacc cctatttttt atgaaaggat tgattacatg aagaaaatac gaagctatac
     1441 cagtatctgg tctgtggaaa aggtactgta ttctatcaat gattttagac ttccgtttcc
     1501 cataaccttt acgcaaatga catggtttgt cgtgtcactc tttgcagtga tgatacttgg
     1561 caacttgccc cctctttcca tgatagaggg agcatttctc aaatactttg ggattcctgt
     1621 ggctttcaca tggtttatgt ctacaaaaac ttttgatggt aaaaagcctt atggattttt
     1681 gaagtctgtc attgcttatg cactgcgacc aaagctgacc tatgcaggaa aaaaagtaac
     1741 gcttggcaga aaccagccac aagaagccat tacagcagtt aggagtgaat tttatggcat
     1801 atccaattaa atacattgaa aacaatctcg tctggaataa agacggggaa tgttatgctt
     1861 actatgagct tgttccttac aattactcat ttctaagtcc agaacagaaa atacaagtgc
     1921 atgattcttt cagacagctt atcgcacaaa atcgtgatgg caaaattcat gctttacaaa
     1981 tcagtacaga atccagcata cgttctgcac aagagcgttc caaaaatgaa gtcactggca
     2041 agctcaaagc ggttgcctat gacaaaatcg accaacagac agacgcttta atatccatga
     2101 ttggcgaaaa tcaagtgaac taccgtttct ttatcggctt taagttgctt ctcaacgatc
     2161 aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa tgctttgtct gattttgtct
     2221 atgatgtgaa ccataagctg atgggcgatt ttgttagtat gagtaatgat gaaatcctgc
     2281 gttttcagaa gatggaaaag ctcttagaaa ataaaatctc tcgtcgtttc aaaatccgca
     2341 ggttagataa ggacgacttc ggctatctga ttgaacacct ttacggacag acaggcactg
     2401 cctatgaaga gtatgagtac catctatcaa agaaaaagct ggataatgaa acgctgatta
     2461 aatactatga cttgattaag cctactcgct gtttggtgga agaaaaacag cgatatttga
     2521 aaatccagca ggaagatgaa accgtctatg tagcttactt taccattaac agcattgtcg
     2581 gagaactgga cttcccgtcc tctgaaatct tctactacca gcaacagcaa tttacattcc
     2641 cgattgatac gtcaatgaat gtggaaattg tagcgaatcg taaagcccta tctactgtcc
     2701 gcaataaaaa gaaagaactg aaagacttgg ataaccacgc ttggcaaagt gataatgaaa
     2761 ccagctccaa tgtggcggaa gctctggaaa gtgtgaatga gctggaaacc aatttagacc
     2821 aaagcaagga atctatgtac aagctgtctt atgtggtaag ggtatcagca aatgatcttg
     2881 acgaactcaa acgtcgttgt aatgaagtga aagattttta tgacgattta agcgtaaaac
     2941 tggtacgacc atttggggat atgctcggct tacatgaaga atttttacct gccagcaagc
     3001 gttatatgaa tgattatatt caatacgtga cctctgattt cctcgctggt ttaggttttg
     3061 gtgctactca aatgctgggg gaaaatgagg ggatttatgt tggctacagc ttagatactg
     3121 gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg ggttaagggt tcagtaacca
     3181 atgcgttagc gtcggctttt gttggttcgc tgggtggtgg taaatccttt gcgaataacc
     3241 ttatcgtcta ttatgcggtg ctttatgggg cacaagcagt gattgtagac ccaaaagcag
     3301 gtgcgaaacg ttcctacttg aaaagagtag gcacagcact tcattaaatc aatatttaat
     3361 gtgtgttgga gaactaaaca cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta
     3421 ctttaatact ctgactggca gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa
     3481 gtcggcagac agacaccgta acagataatg ctgacgaaag attcccagag gtgggcgttg
     3541 tgtcctatat gccggaagtc tataaaatat ctatggttag aatgtagatg agaggtctac
     3601 tgacgaatcc ttgaatgtac aggtctatat cggggctgga tagaaatgtc cagtgacaca
     3661 aacagtgtcg ctactgtggg tgagtaagac tccgcattta tgaaaatcca tataacgtta
     3721 aaggcgttat caaggtagca ggcttataga ggaaacctaa ggatatatgt acagatagtg
     3781 tgtttggaac gttgaaagct gacaacatgg agattttact gtctgtgaag tgttcacatc
     3841 agaaagttat tgtgagatta gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg
     3901 cacagtacct atgaaaccgt gataacaagc ggtggaggga tagccactag tcatatgaag
     3961 aatagaatta ctaagaaaaa tggtatgggt tcgagtaaga ctaagagatg tacttgctcc
     4021 tgcaaacaag gagggtacag accatgctaa agaaaaacaa gctccgctat aacgagtatt
     4081 atgatatgca acatatttat gacgagttat acgcacagag caagaacggt aataactttt
     4141 ataagttact tgaaattatt ggttcggaac aaaatatttg tttagcttac cgtaatctga
     4201 aatcaaacag tggcagtaaa acggctggaa cggatggaat gacgatagac gatataaaac
     4261 aactcagcaa tgctgagatt gttgctaccg ttcgggaaag ccttagcaac taccgcccca
     4321 aatcagtcag acgtgttttc attccaaaag ctgggagcga taaaatgcgt ccattgggga
     4381 ttccgtgtat ctgggacagg ctggtacaac aatgtatcct gcaagtccta gagccgatat
     4441 gcgaaccaaa gttccataat cattcctatg gattccgtgc gaatcgaagt gcccaccatg
     4501 cagtcagcag agtaacgaca ctgataaatt tatcaaaata tcattactgt gttgacgtag
     4561 acattaaagg atttttcgat aatgtcaatc atggaaaact attaaaacaa atctggacac
     4621 ttggtattcg ggataaaaga ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg
     4681 acggcgaggg tgtcccagaa aaaggaacgc cacaaggcgg tctgttaagt ccactgctct
     4741 ccctcatagt ccttaatgaa ttagactggt gggtaagcag tcagtgggaa acattccagc
     4801 cgaagaaccg cagtaaaaat gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa
     4861 gcggttttat tgtacgttac gctgatgatt ttaaaatcat gtgttcaacc tatggagaag
     4921 cacaaagatt ttaccacagt accgtagatt ttcttaataa aaggctgaaa ttagaaatca
     4981 gtccagagaa atcaaaggta gtaaatctga agaaaaattc atcagatttt ctggggttca
     5041 aaatcaaagt cattccaaaa ggaagaacga aacacggtta tgttgccaaa acagatatga
     5101 accaaaaagc attaaagaaa gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca
     5161 gacatacgac tggcttcaat atcagcagat ataatctgac agttatcggt atgcagaatt
     5221 actactgcat agcaactaac gtatataaca acctgacaga agtaagttac gctctcttac
     5281 cgaccatacg aatccgttta aggaacattg caaaatcagt tccatttgaa agtacaagtt
     5341 cagaatttca gagcaggaca aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg
     5401 ataatccgct attgccgatt caaggagtac aacacaaaaa tcctatgaat ttctcgcaag
     5461 atatttgtaa ttttacaaag caagggagaa acaaggtgca tgaggacgtt gtagttgtaa
     5521 caaaagaaga aatccgagcg ttgttggaaa atgaaaaccc tgccgatagc gtagagttta
     5581 acgacaatcg tatttcggca tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc
     5641 gaggaactcc gtccactctg atatgtattc acaaatcaga caaaggagat aaccttgaca
     5701 gatattctaa tctggcattt gtggaaattc ccattgcaaa ggcaatccta acagaatcag
     5761 cagacgaagc aaaatcactg ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac
     5821 tgaacagaat acggacgaat tacggatacc agacaataac tgggaaataa cttataaatc
     5881 tatttggata tgatggaacg ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg
     5941 gaaaacttgg agataacatc aaaaagttac ctatcgctat aacgtggcag atggaaagaa
     6001 accttgccag agatttccca tgaaatcaat atcgtcactc tgacttctga tgagaaaaac
     6061 aaaggcttac ttgaccctta tgtgattatg aaaaatccca aagattctga atcactggct
     6121 attgatattc tgacattcct tacggggatt tcctctcgtg atggggaacg cttcccaatc
     6181 cttagaaaag ccattcgtgc agtaaccaat agtgaagtac gagggttgat gaaagtgatt
     6241 gaggaattac gggttgagaa tacgccacta agtaccagta tagccgacca tatcgaaagt
     6301 tttacagact atgactttgc acatttatta ttcagtaatg gttatgtgga gcagtctatc
     6361 agcttagaaa aacaactgaa cattatacag gttgcggact tggtacttcc cgacaaggaa
     6421 acttcctttg aggaatatac cactatggag cttttatccg ttgctatgct gattgtcatt
     6481 agtacctttg ctttagactt tatccataca gaccgaagca ttttcaagat tgtagattta
     6541 gacgaagcat ggagcttttt acaggtagca caaggaaaaa cactatctat gaagctggtt
     6601 cgggctggtc gtgctatgaa cgctggggta tatttcgtga cccaaaatac agacgacctc
     6661 ttagatgaaa aactgaaaaa taacctcggc ttaaaatttg catttcgttc cactgacctt
     6721 aacgagatta aaaagacctt agcctttttt ggtgtagacc cagaggacga aaacaatcag
     6781 aagcgattgc gtgatttgga aaacgggcaa tgccttatca gtgatttata tggtcgtgtc
     6841 ggtgtgatac agttccaccc tgtatttgaa gaactgctcc atgcctttga taccagacca
     6901 cctgtgcgaa aagaggtgta aatgtgaaac catcaatagt aaacagaata aaatcaaact
     6961 ggacgctgaa acgtctaggt aaagtggcaa tgacagtggc tttcacactt gtgattgcca
     7021 tttttctttt agccatgctg ggaacggtgg ttcaagctgc gggcttggta gatgatacgg
     7081 tcaatgtggc aaatgaatac agccgatacc cacttgaaaa ctatcaactg gatttttatg
     7141 tggataatag ctggggctgg cttccgtgga actggtcgga cgggattgga aaacaggtca
     7201 tgtatggact atatgccatt accaatttta tttggacaat cagtttgtat gtttccaatg
     7261 cgacaggtta cttagtacag gaagcctatt ccttagactt catttccgct acagcagatt
     7321 ccattggtaa gaatatgcag accttagctg gtgtgagtgc aaacggattt tcaacagagg
     7381 gtttctatgt tggattcctc ttactcttga ttttggttct tggggtttat gttgcctata
     7441 cgggactgat aaagagagaa accacaaagg caattcatgc cattatgaat tttgtgctgg
     7501 tgtttatcct atcggcttcc tttattgcct acgctcccga ctacattaaa aaaatcaatg
     7561 acttttcatc agacatcagt aatgccagtt tatcacttgg cacgaagatt gtcatgcccc
     7621 attccgatag tcaaggcaag gacagcgtgg acttaatcag agatagcctg ttttccatac
     7681 aggttcagca accgtggcta ctgcttcaat acaacagttc agacattgaa agtatcggta
     7741 ttgaccgtgt ggaaagcctg ctctccacca gcccagattc caacaatggc gaagacagag
     7801 aaaaaattgt tgcggaagaa attgaagaca gaagcaatac caatctaacc attacaaaga
     7861 ccattaaccg tttaggtaca gtcttcttcc tatttgtctt caatattggg atttccatat
     7921 ttgtattcct attaacagga atcatgattt tctcgcaggt actttttatc atctatgcta
     7981 tgtttctgcc tgtgagcttt attttaagca tgattccatc atttgatggt atgtcaaaac
     8041 gagccataac aaagctcttt aataccattt tgacacgagc tggaatcaca ttgattatta
     8101 cgacagcatt tagtatttca accatgctct ataccttatc ggctggttat ccgttctttt
     8161 tgattgcttt tctacagatt gtgacctttg caggaatcta cttcaagctg ggcgatttaa
     8221 tgagtatgtt ttctctacag agtaacgatt ctcaaagtgt gggaagtcgt gtgatgagaa
     8281 aacctcgtat gcttatgcac gctcacatgc accgtctaca gcggaaactt ggacgttcca
     8341 tgactactct aggggctggg tctgccattg ttacaggtaa aaaaggacag tcgggttcgg
     8401 ggagttctgc aaggacacaa gcagatcact cccgaccaga cggaaaggaa aaatcaacac
     8461 ttggaaaacg tatcggtcaa accatcggta cagtagctga taccaaagac agaatggtag
     8521 acactgctag tggtttgaaa gaacaggtta aagatttgcc gaccaatgca agatatgcag
     8581 tatatcaagg aaaatccaaa gtaaaagaga atgtccgtga tttaaccagt agtatttctc
     8641 aaaccaaagc ggacagagcc agtggacgca aggaacagca ggaacaaagg cgaaaaacca
     8701 ttgcgaagcg tcgctctgaa atggaacagg tcaaacagaa aaaacagcct gcttcttctg
     8761 ttcatgaaag accgactaca agacaagaac aatatcatga tgaacagacc tcaaaacagt
     8821 ctaatattca gacttcatat aaggaatctc aacaagccaa acaagagcgt ccagcagtta
     8881 agtccgattt ttcaagtcca aaagtggaac gccaaggcaa taccgttcaa gaaaaaaccg
     8941 ttcaaaagcc agcaacttca accactacag cagatagaac ttcacaacgt ccaatcacaa
     9001 aagaacgtcc gtctactgtt caaagagtac cactacaaaa tacaagaagt agaccaccaa
     9061 tcaaaaccgc caccattaag aaagtcggta agaaaccatg aagttgaaaa ctttagtgat
     9121 tggtggttct ggattattct tgatggtctt ctcactgctt ctgtttgttg ccattttatt
     9181 ttcagatgaa caggacagcg gaatttccaa tattcattat ggaggtgtga atgtttccgc
     9241 agaagtgctg gctcataagc ctatggtaga aaaatatgcc aaagaatatg gcgttgaaga
     9301 atatgtcaac atacttcttg cgattataca ggtggaatcg ggcggtactg cggaagatgt
     9361 tatgcagtcc tcggaatccc tcggtcttcc acctaattca ttgagtacag aagaatccat
     9421 taagcaaggt gtgaagtatt tcagtgaatt attagccagt agcgaaaggc tcagtgtaga
     9481 tttagaatcg gttatccagt cctacaatta tggtggtggt ttcttagggt atgtggctaa
     9541 tcgtggaaat aaatatacct ttgaactggc tcaaagtttc tcaaaagagt attcaggtgg
     9601 cgaaaaagtg tcttacccca atcccatagc catacctatc aatgggggct ggcgatacaa
     9661 ctatggcaat atgttttatg tgcaactggt aacgcagtat cttgtcacaa cagagtttga
     9721 tgatgatacg gtacaagcca tcatggacga agcactgaaa tatgagggct ggcgatacgt
     9781 ttacggtgga gcttccccga ctacttcttt tgattgtagc ggactgacac aatggacgta
     9841 tggaaaagct ggaattaact taccacgaac cgcacaacag caatatgatg tgacccagca
     9901 tatcccacta tcggaagcac aagctggcga tttggttttc tttcattcta cctataacgc
     9961 tggctcttat attactcatg ttgggatata ccttggcaat aaccgtatgt ttcatgcagg
    10021 cgacccaatc ggttatgccg acttaacaag cccctactgg caacagcatt tagtgggagc
    10081 aggacgaatc aaacaatgag aaaggaagat ttaatgatga aatttagaaa aaatcagaat
    10141 aaagaaaaac agataccaaa ggaaaagaaa cctcgtgtct ataaggtcaa tcctcataaa
    10201 aaggttgtga ttgccttgtg ggtactttta gggcttagtt tcagctttgc gatattcaag
    10261 cactttacag ctatagatac tcatactatt cacgaaacaa ctatcataga aaaggaatac
    10321 gttgatactc atcatgtaga aaattttgta gagaactttg cgaaagtcta ctattcatgg
    10381 gagcaatccg ataagtccat tgataatcga atggaaagtc taaaaggcta tctgacagat
    10441 gaacttcaag ctctcaatgt tgatacagta cgcaaagata ttcctgtatc gtcttctgta
    10501 agaggatttc agatatggac ggtagagcca actggcgaca atgagtttaa tgtaacctac
    10561 agtgtagacc agctcattac agagggagaa aatacaaaga ccgtccactc tgcttatata
    10621 gtgagtgtct atgtagatgg ttctggaaat atggtactgg ttaagaatcc gaccattacc
    10681 aacataccta agaaatcaag ttataaacca aaagccattg aaagtgaggg gacggttgat
    10741 tccattacaa ccaatgaaat caatgagttt ttaacgacgt tcttcaagct ctatcctaca
    10801 gcgacagcca gtgaactttc ctactatgtg aatgacggga tattaaaacc aatcggaaaa
    10861 gagtacatct ttcaagaact ggtaaatcct attcacaatc gtaaggataa tcaagtcacg
    10921 gtatcgctga cagtggagta tatcgaccag cagaccaaag caacgcaggt atctcaattt
    10981 gatttggtac ttgaaaagaa cgggagtaat tggaagatta tagaataaca aatattggta
    11041 cattattaca gctattttgt aatcacgtac tctctttgat aaaaaattgg agattccttt
    11101 acaaatatgc tcttacgtgc tattatttaa gtatctattt aaaaggagtt aataaatatg
    11161 cggcaaggta ttattaaata aactgtcaat ttgatagcgg gaacaaataa ttggatgtcc
    11221 ttttttagga gggcttagtt ttttgtaccc agtttaagaa tacctttatc atgtgattct
    11281 aaagtatccg gagaatatct gtatgctttg tatgcctatg gttatgcata aaaatcccag
    11341 tgataagagt atttatcact gggattttta tgcccttttg ggcttttgaa tggaggaaaa
    11401 tcacatgaaa attattaata ttggagtttt agctcatgtt gatgcaggaa aaactacctt
    11461 aacagaaagc ttattatata acagtggagc gattacagaa ttaggaagcg tggacaaagg
    11521 tacaacgagg acggataata cgcttttaga acgtcagaga ggaattacaa ttcagacagg
    11581 aataacctct tttcagtggg aaaatacgaa ggtgaacatc atagacacgc caggacatat
    11641 ggatttctta gcagaagtat atcgttcatt atcagtttta gatggggcaa ttctactgat
    11701 ttctgcaaaa gatggcgtac aagcacaaac tcgtatatta tttcatgcac ttaggaaaat
    11761 ggggattccc acaatctttt ttatcaataa gattgaccaa aatggaattg atttatcaac
    11821 ggtttatcag gatattaaag agaaactttc tgccgaaatt gtaatcaaac agaaggtaga
    11881 actgtatcct aatgtgtgtg tgacgaactt taccgaatct gaacaatggg atacggtaat
    11941 agagggaaac gatgaccttt tagagaaata tatgtccggt aaatcattag aagcattgga
    12001 actcgaacaa gaggaaagca taagatttca gaattgttct ctgttccctc tttatcatgg
    12061 aagtgcaaaa agtaatatag ggattgataa ccttatagaa gttattacta ataaatttta
    12121 ttcatcaaca catcgaggtc cgtctgaact ttgcggaaat gttttcaaaa ttgaatatac
    12181 aaaaaaaaga caacgtcttg catatatacg cctttatagt ggagtactac atttacgaga
    12241 ttcggttaga gtatcagaaa aagaaaaaat aaaagttaca gaaatgtata cttcaataaa
    12301 tggtgaatta tgtaagattg atagagctta ttctggagaa attgttattt tgcaaaatga
    12361 gtttttgaag ttaaatagtg ttcttggaga tacaaaacta ttgccacaga gaaaaaagat
    12421 tgaaaatccg caccctctac tacaaacaac tgttgaaccg agtaaacctg aacagagaga
    12481 aatgttgctt gatgcccttt tggaaatctc agatagtgat ccgcttctac gatattacgt
    12541 ggattctacg acacatgaaa ttatactttc tttcttaggg aaagtacaaa tggaagtgat
    12601 tagtgcactg ttgcaagaaa agtatcatgt ggagatagaa ctaaaagagc ctacagtcat
    12661 ttatatggag agaccgttaa aaaatgcaga atataccatt cacatcgaag tgccgccaaa
    12721 tcctttctgg gcttccattg gtttatctgt atcaccgctt ccgttgggaa gtggaatgca
    12781 gtatgagagc tcggtttctc ttggatactt aaatcaatca tttcaaaatg cagttatgga
    12841 agggatacgc tatggttgtg aacaaggatt gtatggttgg aatgtgacgg actgtaaaat
    12901 ctgttttaag tatggcttat actatagccc tgttagtacc ccagcagatt ttcggatgct
    12961 tgctcctatt gtattggaac aagtcttaaa aaaagctgga acagaattgt tagagccata
    13021 tcttagtttt aaaatttatg cgccacagga atatctttca cgagcataca acgatgctcc
    13081 taaatattgt gcgaacatcg tagacactca attgaaaaat aatgaggtca ttcttagtgg
    13141 agaaatccct gctcggtgta ttcaagaata tcgtagtgat ttaactttct ttacaaatgg
    13201 acgtagtgtt tgtttaacag agttaaaagg gtaccatgtt actaccggtg aacctgtttg
    13261 ccagccccgt cgtccaaata gtcggataga taaagtacga tatatgttca ataaaataac
    13321 ttagtgtatt ttatgttgtt atataaatat ggtttcttgt taaataagat gaaatatttt
    13381 ttaataaaga tttgaattaa agtgtaaagg aggagatagt tattataaac tacaagtgga
    13441 tattgtgtcc tgtatgtgga aataaaacac gattaaagat aagggaagat actgaattaa
    13501 aaaaattccc cctctattgt ccgaaatgca gacaagaaaa tttaattgaa ataaagcagt
    13561 tcaaagtaac tgtgattaca gagccagacg caaagacgca gagccgataa aatgagatta
    13621 atacaatctc attttatcgg ctctttccgt tatgtatgga ttcttttaat tagtcttcga
    13681 tgtttcttgc ttcgttgata ccgctggcta aagattccat tatggatagt tctttgtctg
    13741 taaagctatc catgtatttc tctatctgta atcgtcgggt gctttttacc aagttattag
    13801 caggtaagaa aaattcatca acggaaacat gaagtaacga tacaaggtca taaagaactt
    13861 gtatgctggg gtgttgccct ttattttcaa tattagttaa gtaccgtggg tcaatttcaa
    13921 tcaatgctcc cacttgttca cgagttaaac ctcgtttcaa tcgagcttct ttaatggcta
    13981 aaccaaaggc tctaaaatca tatttatctt cttttttacg catagtagac cacctctata
    14041 cattttattg ttcctactga attaaaaaca ggtatagaaa aacgtgttat atggtttata
    14101 ggtttata
//
LOCUS       DAMYXM010000064        14050 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.065, whole genome shotgun sequence.
ACCESSION   DAMYXM010000064 DAMYXM010000000
VERSION     DAMYXM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.065"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..911
                     /locus_tag="PNS21_002164"
     CDS             <1..911
                     /locus_tag="PNS21_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HDI5159863.1"
                     /translation="KTVGKVPVDLGWKKLFGKESDRKEKEEEPLLPKVTKGEAVTVDL
                     QVLEKETKPPQPYTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEATRANIIETLK
                     QKEYIKVEKNKLVVTNKGILLCQAVEKEPLLTSAEMTAKWESYLLKIGEQKGTQATFL
                     ANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKAASEKQEAARHLGICPKCQEQEVLLYH
                     KAAACTSEACDFRLWTTIAKKKLTATQLKEIIQNGRTSQPVKGLKGQKGSFEATIVLK
                     EDFTTSFEFSEKKKTNYKKRTRRTTK"
     gene            927..1076
                     /locus_tag="PNS21_002165"
     CDS             927..1076
                     /locus_tag="PNS21_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159864.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            1157..1753
                     /locus_tag="PNS21_002166"
     CDS             1157..1753
                     /locus_tag="PNS21_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDI5159865.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYSKAEWYELGKIEIKSRYGNPVIAYDPERTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTIRSYIMALN"
     gene            1766..2665
                     /locus_tag="PNS21_002167"
     CDS             1766..2665
                     /locus_tag="PNS21_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDI5159866.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFENRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYEETMDSISNLMEKVI
                     HVEEKERKIAYQRHQQMNREMER"
     gene            2668..3153
                     /gene="ssb"
                     /locus_tag="PNS21_002168"
     CDS             2668..3153
                     /gene="ssb"
                     /locus_tag="PNS21_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI5159867.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGILIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            3519..3779
                     /locus_tag="PNS21_002169"
     CDS             3519..3779
                     /locus_tag="PNS21_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159868.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            4226..4432
                     /locus_tag="PNS21_002170"
     CDS             4226..4432
                     /locus_tag="PNS21_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HDI5159869.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            4432..4656
                     /locus_tag="PNS21_002171"
     CDS             4432..4656
                     /locus_tag="PNS21_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159870.1"
                     /translation="MKTQHKLLNVKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFE
                     DLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            4671..5108
                     /locus_tag="PNS21_002172"
     CDS             4671..5108
                     /locus_tag="PNS21_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159871.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEAGVKPSTIKPSEYQALE
                     LTYGKFIETKKAKTVSSEWLDYFENVNKYGACYIMKKEDNENE"
     gene            5101..5808
                     /locus_tag="PNS21_002173"
     CDS             5101..5808
                     /locus_tag="PNS21_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159872.1"
                     /translation="MSNVVYQLTVEDVSHFLTNKEHLNEVVRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDCKRMPYQRFERKKGQ"
     gene            6189..6368
                     /locus_tag="PNS21_002174"
     CDS             6189..6368
                     /locus_tag="PNS21_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159873.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQELKQKYGKSESRIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            6461..6646
                     /locus_tag="PNS21_002175"
     CDS             6461..6646
                     /locus_tag="PNS21_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDI5159874.1"
                     /translation="MPLTGKEMLKLLKKNGWVERRQEGSHHHLYKDGVRITVPVHANQ
                     DLGRGLEQKILKDAGLK"
     gene            6680..7111
                     /locus_tag="PNS21_002176"
     CDS             6680..7111
                     /locus_tag="PNS21_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDI5159875.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
     gene            8048..9322
                     /locus_tag="PNS21_002177"
     CDS             8048..9322
                     /locus_tag="PNS21_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5159876.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            9376..10803
                     /locus_tag="PNS21_002178"
     CDS             9376..10803
                     /locus_tag="PNS21_002178"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDI5159877.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDHLYMKPVRELEQLRVG
                     SGVETKVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            10816..11688
                     /locus_tag="PNS21_002179"
     CDS             10816..11688
                     /locus_tag="PNS21_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDI5159878.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNELLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            11783..12778
                     /locus_tag="PNS21_002180"
     CDS             11783..12778
                     /locus_tag="PNS21_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI5159879.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKDN"
     gene            complement(13383..13490)
                     /locus_tag="PNS21_002181"
                     /pseudo
     CDS             complement(13383..13490)
                     /locus_tag="PNS21_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            13606..>14050
                     /locus_tag="PNS21_002182"
     CDS             13606..>14050
                     /locus_tag="PNS21_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDI5159880.1"
                     /translation="MIIKNLGNVKSIDIHVKDLLIFTGKNNTGKTYTSYILYGILSEL
                     FGARFFNYFSHTEIEPHLQNSLNILLVSKDSIRKKFNNDAKKFLEENLKKIAVRNFKV
                     SENNFKNLSFTISDSEISELTGDIFNYEIKRYSISGIVITIQNVGE"
BASE COUNT         5012 a         2147 c         2760 g         4131 t
ORIGIN      
        1 taaagacagt cggaaaagta ccagtagatc taggttggaa aaagctgttt ggcaaagaga
       61 gcgacagaaa agaaaaggaa gaagaaccgc tacttcctaa agtgacaaaa ggagaagcgg
      121 tgacggtaga tctacaagtc cttgaaaaag aaacgaagcc accacaacct tataccgaag
      181 gtacgttgat cacagcgatg aaaacagctg gaaaaaccgt ggatagcgaa gaagcacaat
      241 ccatattgaa agaagtagaa ggaatcggta cagaagcaac acgagcaaat attattgaaa
      301 ctttgaaaca aaaggaatat atcaaggtag aaaaaaataa gcttgtggtg acaaataaag
      361 ggattttact ttgccaagcc gtggaaaaag agccgctgct aacaagtgcc gaaatgaccg
      421 ccaaatggga aagttactta ttaaagattg gtgagcagaa aggaacacaa gctactttct
      481 tagctaatat ccaaaaattt gtctctcact tgttggaagt cgtaccagga caaatccaat
      541 ccactgattt tggatcgacg ttacaagagg tgaaagcagc cagtgaaaag caagaagccg
      601 cgcgtcattt agggatttgt ccaaaatgcc aggaacaaga agttttactc tatcataagg
      661 cggccgcatg tacgtctgaa gcttgtgatt ttagattatg gacaacgatt gccaaaaaga
      721 aactcacagc gacacagctg aaggaaatca ttcaaaatgg ccgaacaagc cagccagtga
      781 aaggattaaa aggtcaaaag ggttcgtttg aagcaacgat tgttctaaaa gaagacttca
      841 caacgagctt tgaattttct gaaaagaaaa aaacgaacta taagaaaaga acacgccgca
      901 ctacaaaata atggaggggt tatcttatgc gaaatacaac acacaaacaa caagaaatcg
      961 aaaatcaaaa agcaaaaatt cgttcagaac aagagaaatt acgtgccagt aaaagaagac
     1021 ttcagcaatt aaagaaccag caacggattc aaaaagaaat tgaattagat cgataaaatc
     1081 ttcctggcta ttgtatacgc ataaacttat gttatactat atttataagt ttatacgtat
     1141 acaaaggagg gagaaaatga caaaagcaat ggtttacaag ctgctagaag aatttaacgg
     1201 aactttggct ttgaaggatg ccaaaaaagc gggcatttct cctgtaacga tcaaacgtat
     1261 ggtagatcgt ggagagctgg atcgtgagta tccaggtttt ttcaccttac ctggtcaatt
     1321 tcctgatgaa ttgtttatgg cacaaaagaa atatgagcgc ggcattattt cgcatatcac
     1381 cgcgttggat ttatatgatt taacggatat gatccctaga cagatcgatc taaccgtacc
     1441 ttacggttat cacgtttctg aaaaggggtt gaaagaattc gcagttgagt tacactattc
     1501 aaaagcggaa tggtatgaat taggtaagat cgaaataaag agtcgttatg ggaatcctgt
     1561 tatagcttat gatccagaaa gaaccttgtg tgacatttgg aatccctggt acaacgttga
     1621 ggatgaaatc aaagtaaaag caatcaaaaa ctacatggag tcggacagaa aaaatcttag
     1681 aaaattgaat gagtatcgac gaattttgcc tacagacaaa acgatacgct cttatattat
     1741 ggctttaaat taaaggagaa gacacatgac acctacgcaa ttaaaagcaa aagtgaagaa
     1801 tatttccaga gaaaaagaag ttgatccgca gcttgtttta agacatttta tgatggaaaa
     1861 attcttggaa aaaatctcag agtctcctta tcgtgaaaat tttgtgttaa aaggcggctt
     1921 cttaattggc tcaaagtatg gcattgaaaa tcgaacaaca aaagacattg atacgacttt
     1981 acgggaaatg aaagtaacca aagaaacatt gacaacggtt ctaaacgata ttttttcaac
     2041 gccgacgaaa gaaggcattc aatttgagat tcaaggaatg aaagaaacaa gggaagctga
     2101 ttattatcca gggtttagct tacgtgtact ggcacatttg gaaaacatgc ggccagattt
     2161 taaggtagac gtgacgacag gtgactcgat ctatcctgca acgatcactc acagtcataa
     2221 attgatgttt gaaaatcgaa cgattccttt ggaatcttat cctacagaac aaattattgc
     2281 cgaaaaatta agtgcaacct ttgatttttt aacagataat tcgagaggaa aagattttta
     2341 tgatctctat actattccta aaatggaaaa gatcgatgag aagaccttga aagatagtgt
     2401 gagaaataca tttacacgtc gaggaaaaac tgatccgtta aagatttatt acgaaaagga
     2461 tatggatgta ctgaaaaata gtcatgagct aaaagatacc tggaagaaat atcaaaaaag
     2521 caatccttat gctgcgtcga tcacgtacga agagacaatg gattcgatta gtaatttgat
     2581 ggaaaaagtg attcacgtcg aagaaaaaga gcgaaaaata gcttatcaaa gacaccaaca
     2641 gatgaacaga gaaatggaga gataaaaatg atcaataatg taactttagt aggacggtta
     2701 accaaagatc cagatttacg ttataccgca agtggcacag cagtggctac ttttacccta
     2761 gccgttaatc gtaatttcac gaatcaaaac ggcaatcgag aagctgattt tatcaattgc
     2821 gtgatttggc gcaaaccagc agaaacgatg gcgacattag ctaaaaaagg catcttgatc
     2881 ggcgttgtcg gacggattca aacacggact tatgacaacc agcaaggaca acgtgtgtat
     2941 gtgacagaag ttgtagctga taacttccaa ctattagaaa gcaaagcagc gacagagagc
     3001 cgtgcgcacg ctgatcaatc gtccacgagt ccaagcacca ccacttttga acaacgggat
     3061 acagcgacgc ctaacaacaa tggattgaac gcttcacaga atccatttgg tggacaatca
     3121 atcgatatta gcgatgatga tctaccgttt taagtagcat tttctattgg ttgcatttgc
     3181 tacggtttat gttataattt agtcaacaaa aagaattttc aatctataac caaaaaccac
     3241 tagctggcgg gctagtggtt ttcttttacg tttctattat aagaattaac gagtaggctt
     3301 actgtctttc ttggcaaatt tacaatcaag ccatttgcag atatagtgcg cggctacttc
     3361 tgccatgatt gcaaaaacaa aatcaaaaag aattttcaaa cctataacct cctccccttg
     3421 ccagattagg ccggggtagc agacagcaag actattatag cataattgaa atatccaaat
     3481 gattggattt ttgctataat tttagcgagg tgattgtgat gaacaaatat gttggtttgc
     3541 ttgataaaat ccgagtaata aaaacgcaac cgttattggt tcgctttact ttacaaacga
     3601 ttcatgaatc gattaattgt gtagttgcag atattgaaat tatcgataaa ttgctaatta
     3661 tggatgatgg aaaatacaat attgccgtca caggtcactt caataaacga aatcagctgg
     3721 ttattgctag catgtatgtt agaaatcccg atcattttac acgatcgatg ggcatataaa
     3781 taaaagaaga gttgtagtcc taaatgagga ttgcagctct ttttgtttta catagaaacg
     3841 ggaacaggaa aaggcctgtt taggaaggca caagcgcctt ctcaattaaa aaacctttct
     3901 ctcactccaa ccgccaccgc cgttcgagaa gaaaggaagt gaacgctgca cgttcactaa
     3961 gatatggtgt cttgcaaagc aagacaaaat aggaaaagct tcctctcacc aaaaggttcg
     4021 ttcctgttcg ctatctcttt attgatttgc cagaccggca acaagttgcc ttttatcacc
     4081 aaaaagcgac aaggacaagg gtaaatggca cgctgcgcgc ccttgtcctt gtcgcttttc
     4141 gttgattcat tctggccaac aaatcaataa agaaagaact aacgaaagga agtaaaacct
     4201 ttcctaagag gaggcagaat caatcatgcg aaagaaattt gattattggg gtgttccctt
     4261 cagcgaatta ttcccaaact atcatgcacc acatactgtc gaatgcgatt gtggagaaag
     4321 agcgaaatgt atcaaaagct atcgtttgta tcaatgtcca acatgcggga aaaaatatac
     4381 cttgtcttat ggcgattaca ttttgattga tgagaaagga aagaaacgct gatgaaaaca
     4441 caacacaaat tattgaatgt aaaagaagtc atttctgaaa aagtagcaga agctttactt
     4501 attccgcggt actggacgat agaaacccct ttagtagagg acgcaccgac tatgctattt
     4561 gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaactt ggaaacttat
     4621 gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaat atggctcaga
     4681 attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa atggaggata
     4741 tgacgttcct ataccatgaa acacgtgtac ccaaaaaaca ttacaaggaa aaattgtcag
     4801 tcgcagtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg acgtactatc
     4861 gaacattgga agaactgaaa aaacaaaatg gaaaatggtt tttccaagcc ttactatgct
     4921 tggaagcagg cgtcaaacca agcacaatca aaccttctga ataccaagct ttagaactaa
     4981 cgtatggaaa atttatcgaa acgaaaaaag ccaagactgt ttcaagtgaa tggttggact
     5041 actttgaaaa tgtcaacaag tatggcgcat gttacatcat gaaaaaggag gacaacgaaa
     5101 atgagtaatg tggtttatca actaacagtg gaagatgttt ctcacttttt aacaaacaaa
     5161 gaacatttga atgaagtagt cagacggatt caaaaagact ttagttgtat ggaagaaatg
     5221 aagtacttta ttcaaagagt ggaagaacag ttgtatatgg aaagcgaaac gagtcaaacg
     5281 aaggtcacag tcagccataa tcatttagac gtgtataaac gtcgtcctga aacggtaaaa
     5341 aaaggaacag agcttctacg actagaaaca gaaaaataca tcatgaatgt gtacctagaa
     5401 aggagtgagc tggtcgaaaa ctttgtcagc tacacgattt gtaggaaaca ctattactca
     5461 gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga aatggaagag
     5521 ctaaacagca aaaatgcgac ggtagacgat tattaccttg tatttactag atttctgacg
     5581 tgtgggacgg aatctatcgt gttaatggga atggattatg aactcgctgt acccgaagca
     5641 tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta cacgacttta
     5701 actgattttt tagataccta cacttacgac gatacagaag aaatccaaaa tgagcttgac
     5761 tgtaaacgaa tgccatatca acgatttgaa aggaaaaaag gtcaataacc atttataccg
     5821 aaaagtgaga atgtgtgaaa taagaacaaa aaaccttgaa attaagggaa tggttcttat
     5881 ttcgcaccaa tcgctccaac cgcccccgcc gctcaggtgg tgcgaaataa gcgggcaacg
     5941 accgataacc taagagaaag aaggaaatcc atatgaataa aatcattcct ctgttgttcg
     6001 tactgtttgt tggcgagata cttatgcgtc atgctgcagc tgtacattta aacttcgtag
     6061 tattcttagg aatactggta ttttgtttga tcttttggcg atttgtatta gcccttgtga
     6121 gtctactatt caagatgatt gcagccatat ttttcatagg gttatttcga tgaaaggaga
     6181 gagtactaat gccaattgca ataggaaata aacgtttacc cgtgacgttg gatgaaaaga
     6241 gacaaaaaga attgcaggaa ctcaaacaga agtacggcaa aagtgaatcc aggattatgt
     6301 gtattgcgtt agatttattg attgcacaag aaaaagcagg gtttgatgta ccagcactca
     6361 aaaagtgacg tcacttttca tttgaagaaa caaaagtgat agcactttac gcacgtgtcg
     6421 atacgtgtta tgattatttt gtaaggaggt atagtgcaca atgccactta cgggaaaaga
     6481 aatgcttaag ttattgaaaa aaaatggctg ggttgagcga aggcaagagg gatctcatca
     6541 tcatctttat aaggatggtg ttagaatcac tgttcctgtt catgccaatc aagatcttgg
     6601 ccgaggactt gagcaaaaaa ttttaaagga tgcgggattg aaataatctc gcattcgatt
     6661 aaagaaagga ttgactgaaa tgttaagtga gcctttagca aaaacctatc ctgctatttt
     6721 tagcccagaa gaaggtggtg ggtactttat tgaatttcct gatgttcaag gtgcttacac
     6781 aggaattaac gaagacgata tatcttatgg aattgctatg gcacaagagg tgttaggtat
     6841 ggttttagca gattatattg agcatgaaga tctacttcca gaaccaacac caatcaataa
     6901 gatttctgtt gaagatgatt cttttactac tttaattcgt gtagatgttg ccaaatattt
     6961 gaaagacaca gaacttgtca aaaaaacctt aacaattcct caatgggcgg ataagctagg
     7021 caaaagggct ggcataaatt tttcagtcct tctaacagaa tcaattgcag ataaagcaga
     7081 taacattctt cgttctggaa gaaataatta gaactcaaaa gagtcagaaa cttaaaaatc
     7141 tttggcttct ttttttacta caaagatggc ttctttaaat tatttgcctg attgatttaa
     7201 aaaaattgcg caaagactac gagctaatta agttgctcgt ggtctttctt tatgaaaact
     7261 ttttagatct agataaaaac attgagacac tttgaaaaat ttttttaata gtaatgggcg
     7321 cttctatgtc aagctgattc ttctttcggt aaattaattt tacttagaat atcactatct
     7381 tgataaaaaa tggtgtattc acaaattctg gcataaaaaa tacctacttt ctagttaagg
     7441 ttcctgaata agtcgcaatc caattatttc cagctgattt ataacttttt aaatataatt
     7501 ttccataata ccaataacca ttactttttt ctgtccaatt tcgttattga gaagatctag
     7561 aaggccagcc aactttatca aaaatcatca ttcctgtcac tcgtatcgtt ttagaagctg
     7621 ctcgagtaag taatggagaa actgttaaat aattttaagt aacagttaag tcgttagttg
     7681 ggttatcaat ggctattccg tccttatctg aacctcgaaa cccaatcata gaaaaagttt
     7741 tttgaacaag ttttaacaga taaatcaagt tatatactag aaaattcgta aaatatattt
     7801 ttaatgtcat aatcgtttga tttttctgga ttgacattta tttataaaaa atagtgccat
     7861 taaaaaagaa aagtgacaca ccaataaaag tcatatcctg tgacattata tggcattatc
     7921 caaatagaat aaccggttga catgtaagcg gattcctttt ataattttag gagtataagt
     7981 ccttttctaa aaatattaac cggttatcat gaaaggggat acaaatatat cgttggagga
     8041 taaaaatatg tcaaaggtta acgtatttaa aaatcaatcc tatttacaaa gttcagctac
     8101 attattacta ttttttgctt cttggggtgt ttggtggtca ttttttcaac tttggctaac
     8161 atctgaatca aatggtttag ggttatctgg cagtgctgta ggaacagtat tctcggcaaa
     8221 ttcgttagtt accttaattt tgatgtttat ttatggaaca ttacaagata aattgtatat
     8281 taaacgaaat ttattaattt ttgcttctgt attagcgaca cttgttggac cattttttat
     8341 atggatatac gggccattgc tagataacaa ttttaattta ggcattatta tgggagcact
     8401 atttttgtca gctggatatt tagcttctgt aggagttttt gaagctgtgt cagaaaggtt
     8461 tagtcgttta tttggctttg aatatggaca agcaagggcg tggggatcat ttggttatgc
     8521 cttggtagcg cttttggcag gatttttatt tgtaaaaaat cctcatttaa acttttgggc
     8581 gggatctttc tttggttctt tactattgtt aaatttatta ttttggaacc ctaaagttga
     8641 acgggaagca aatcaaaatt ttaatcaaga acaagctgaa tcaaatagta ttccttcttt
     8701 aaaagaaatg tttgatctaa tgaaactgcc tcaattatgg acgataatca tctttattgt
     8761 ttttacatgg acattttata cggtattcga tcaacaaatg tttccgggat tttatactgg
     8821 tttgttttca acatcagcta atggtgaaaa aatatatggg acattgaatg ctattcaagt
     8881 attttgtgaa gcgttaatga tgggaattgt tccaatcatt atgagaaaat taggggttcg
     8941 aaatactttg ttattaggtg taaccattat gtgtgtacga attggattgt gcgggtttgc
     9001 ctcgacacca ttatctgttt catgcataaa aatgttgcat gctttagaag taccattatt
     9061 tacattacca atgtttcgct attttacact tcattttgat acaaagctat cagcaaccct
     9121 ctatatgata ggatttcaga tagctgctca aattgggcaa gtgattttat caacaccatt
     9181 gggaatatta agagacaacg ttggctatca accaacattt aaaattattt ctcttattgt
     9241 attactagca ggcatatatg cattctttat tcttaaacaa gatgatagag atgttcaagg
     9301 ggatccattt attcgaggat aatggaaacc aacagaataa aaaacaattt tatctagaaa
     9361 gtgagcgaat atattatgac aaaaagagct atagaattaa caaatacacg ttatcgtttg
     9421 ggatatcatg tgtctgcacc atcaggatgg attaatgatc caaatggatt ttgctatttt
     9481 gatgggtatt atcatgtgtt ttatcaacat catccatata gtgctgaatg gggaccaatg
     9541 cactgggcac atgctagaag taaggattta gttcattggg aatctttacc acttgctttg
     9601 actcctggag atcaagaaga tgaaggtggt tgtttttcag gaagcgctat tgaaaaaaat
     9661 ggtgtgttgt atctgttcta tacaggacat cactattatg gagacaatga tccagatcat
     9721 ttttggcaaa atcaaaacat ggcgtatagt acggatggca ttcactttac taaatatgaa
     9781 aaaaatcctg taatagcaaa agcaccagaa gataacacac atcatttcag agatccaaaa
     9841 gtatggaaac acgatgaaaa atattacatg gttttgggta gtcaagaaaa agatggatta
     9901 ggtcgtgcta ttgtttatca gtcgttagat ttattaaatt gggattacat tggaccaatt
     9961 tctaaagcga aggaagtact tacagaagga tttatgtggg aatgcccaga cttttttgaa
    10021 ttaaatggaa aatctattct tttattatcc cctcagggaa ttgaagcaaa tggaaaagat
    10081 tatttaaatc tatatgaaac aggctacttt atcggtgatt atgattatga gacagcaaaa
    10141 ttcattagag gaaattttta tgaattagat aagggacacg atttctatgc aacacaaaca
    10201 actttatctc cagatggtag acgtattgtt atggcttgga tggatatgtg ggaaagccct
    10261 atgcctgaaa aagtagacgg atgggcagga gcattaacgc ttcctcgaga attagagtta
    10321 attggagatc atttgtatat gaaacctgtg agagaattag aacaacttcg agttggcagc
    10381 ggcgttgaaa caaaagttaa tttggcagga gaaataataa tcgctcaaga tgcttcattc
    10441 aaagaagctc taattgatgt actattgact aaatcaaaaa atcaagaaat caaattttca
    10501 ttaaaaacaa aagatgaaga gttaattaat attttttatt ctaaggataa aaatgaatgt
    10561 accttaactc gtaacggcaa aaatgatcca aggtatggaa ccattcagat gtgtgataga
    10621 ttatcacttc gagtgtttat cgataaaagt tcggcagaga tttttattaa tgatggcgag
    10681 cttgttttta cagagcgtta ttatacagaa aatcaaccaa gtatgcattt gagtgttagt
    10741 gaagaagctt ttgtctcgag tacaatttat catctaaaaa aagatgcaat ttcttacgag
    10801 taaggaggtt ttatgatgtt aattggtagc atagaagcag gtgggactaa atttgtatgt
    10861 gccgtgggag acgaggatta ccgagtacaa gaccaaataa tttgtccaac gaccaccccc
    10921 agggaaacat tgaaaaaaac tgttgactat tttaaacagt ttgatataaa tgcattaggt
    10981 attgcatcat ttggtccaat agaaattcga aaaaattcac caaaatcagg atatattacg
    11041 tctacaccaa aggtgggatg gcgaaatgtt aatgtgttag gttatttaca agatgagctg
    11101 aatgttccaa tgtcttggac aacggatgtg aacggatcag cttatggaga gtacatcatg
    11161 tccactttat cgaatgaaaa aataaattca ttagtttatt acaccgttgg tactggcgtt
    11221 ggtggtggag ctgttgttga cggaaaacta ttaggatcgt ttggtcattc agaaatgggc
    11281 catacgtttg taaaacgcca tcctgatgat attgatttta aaggaatttg tccttatcat
    11341 ggtgattgtt tagaaggctt agttgcggga ccaacgtttg aagcacgttt tggaaaaaag
    11401 ggtgaagacg tttctttaac ggatcctatt tgggatgtaa tggcctatta tctagcacaa
    11461 gctgtgattc aaacaacatt gattatacga ccggataaaa tagtatttgg tggaagcgta
    11521 gtaaatgaaa aattgttaga aaaaattcgt gttaatgtta atgaattatt ggatgattac
    11581 gtggaaatac caccgttaag taaatatatt acgcgtcctt taattaaaaa taatggatcg
    11641 gcaacactag gaaactttgc tttgggcttg aatcaattac aagaataaaa catagaaaaa
    11701 atatcgatta tcaggtatag tagttctata gagttctttt tttcgatagt taattattgc
    11761 attatttagg agagacaaga gaatgcgccc aaaattagaa gatgttgcca aaagagcaaa
    11821 tgtatcaaaa acaactgttt cacgtgtatt aaacaatcgt ggctatttaa gtcaaaaaac
    11881 gattgataat gtttataagg ctatagaaga actcaattat caaccgaacg ttgtagcgcg
    11941 tcaattattt caaaagaaaa ctaacattgt cgggttacta tttcctacag tagccaatcc
    12001 tttttttagt gaactcgttg aggcattgga aaaaaagtta tatgaaattg gttataaggt
    12061 attaataggc aattcgatga ataataaaga aaaggaaaca aattatttaa atcagttatt
    12121 gtctgatcaa gtagatggtt taattgttgg tacacataac caagggattc aggaatataa
    12181 atatcagaat ttaccgattg ttgcaattga tcgagtgatg aatgaagata ttccagttgt
    12241 tgaatccgat aactacaatg gtggaaaact tgcaaccaag ttattaattg cacaaggtgc
    12301 aaaaaatatt attcatacaa acggtccaat tgacttacag actccggcta atcgtcggag
    12361 actagcctat gaagatacta tgaaagcata tcagttaatt ccacgaactg tgacattaga
    12421 tttcaatatt tcttatgtaa agaaaaaaca gatttttttc caaatgtttg aggattatcc
    12481 taaaatagat ggtatttttg cttcaaatga catcgatgcg gccctaatat tacaagtagc
    12541 aaaagaaaag gggttaaatg taccagctga tttattagta gtcggatatg atggtacttt
    12601 aatgactcgg agtatattac cagatctgac aacagtaata caaccaatta atgatatagc
    12661 agacacagct gttgctattt tgatgaaacg aataaataaa gaagaaacaa aaaaggaata
    12721 tattttacct gtcacattat ggatggggaa gacatcagta aagaaaaagg acaattaaat
    12781 ttataaagac tatcaatgat aatttcatta ttaattcgtg atataaaaag ttgttaaatc
    12841 tcttataata gactatgata caaaatcatg cttttaatct gtgtagatgt tgccaaatat
    12901 ttgaaagaca tagaacttgt cagaaaaacc ttaacaattc ctcaatgggc ggataagcta
    12961 ggcaaaaggg ctggcataaa cttttcagtc cttctaacag aatcaattgc agataaagca
    13021 gataacattc ttctttctgg aagaaataat tagaactcaa aagggtcaga aacttaaaag
    13081 tttttgatcc tttattttca agtctgattt gacagatttt cggcagctca tttatttatg
    13141 gtcagaaata gttgctgaaa ttttaatcta ttagcaaata gtccagaacg tatgaagagg
    13201 attcggatat catttgtcat taacaccata aatatttata aaattcgtgt tcacctacaa
    13261 ttaaatttaa tatataatat tcgttgtaag ggcttataat tttttgttaa tgaacaagat
    13321 atgtaattga tagtgtcaat agctattcaa aaatgtaggc ttttggccca taaataacaa
    13381 ggttacttat atgataactt tttttcttta taatctttaa tccgatagag ctttccagta
    13441 gtttgaataa tcttagcgtg gtgcactaat cgattgatta gtgcatttgt gttcagtcca
    13501 ttgagacata atagtggata gttttactga gaaaattgat aaaaaataga gatataattc
    13561 aaatacgtat ataatgaaac taaagataaa tacggaggaa aatatatgat tataaaaaac
    13621 ttaggaaatg ttaaatcaat agatatacac gttaaagatt tgctgatttt tactgggaaa
    13681 aataatacgg gaaaaacata taccagctat attttatatg gaattttatc agaactcttt
    13741 ggagcaagat ttttcaatta tttttctcat acagaaattg aaccacattt acaaaatagt
    13801 ttaaatattt tattagttag caaagactct ataaggaaaa aatttaataa tgatgcaaaa
    13861 aaatttttag aagaaaattt aaaaaaaatt gcagttcgta actttaaagt aagtgaaaat
    13921 aacttcaaaa atctttcttt tactatttca gattcagaaa ttagtgagct cactggtgat
    13981 atctttaatt atgagatcaa aagatacagt ataagtggca ttgttataac aattcaaaat
    14041 gtaggagaga
//
LOCUS       DAMYXM010000065        13747 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.066, whole genome shotgun sequence.
ACCESSION   DAMYXM010000065 DAMYXM010000000
VERSION     DAMYXM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.066"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..863
                     /locus_tag="PNS21_002183"
     CDS             <1..863
                     /locus_tag="PNS21_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI5159881.1"
                     /translation="MSQMMDFLSIFTDGMVAGSRILKIMDTEEYAPQQNPGADQMITS
                     GKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGSGKSSIINVLMRFYEFYEG
                     QILIDDHDIREYPMEELRKKMGMVLQDAFLFYGDIAGNIRMMNPAITDEQIRHAAEFV
                     QANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVTDPKILVLDEATANIDTET
                     EGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGHIVEHGNHETLLAQNGLYA
                     DMYKLQSSGI"
     gene            1189..2040
                     /locus_tag="PNS21_002184"
     CDS             1189..2040
                     /locus_tag="PNS21_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDI5159882.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEDLQVHLNDSVYIALTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSIHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFSCIEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            2137..4017
                     /locus_tag="PNS21_002185"
     CDS             2137..4017
                     /locus_tag="PNS21_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDI5159883.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVPFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTETAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYTMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(4057..4959)
                     /locus_tag="PNS21_002186"
     CDS             complement(4057..4959)
                     /locus_tag="PNS21_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDI5159884.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            5190..6593
                     /locus_tag="PNS21_002187"
     CDS             5190..6593
                     /locus_tag="PNS21_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDI5159885.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            6593..8026
                     /locus_tag="PNS21_002188"
     CDS             6593..8026
                     /locus_tag="PNS21_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDI5159886.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQVKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            8171..9097
                     /gene="trxB"
                     /locus_tag="PNS21_002189"
     CDS             8171..9097
                     /gene="trxB"
                     /locus_tag="PNS21_002189"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDI5159887.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            9311..11038
                     /locus_tag="PNS21_002190"
     CDS             9311..11038
                     /locus_tag="PNS21_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDI5159888.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEEAKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            11053..11403
                     /locus_tag="PNS21_002191"
     CDS             11053..11403
                     /locus_tag="PNS21_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDI5159889.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(11495..12355)
                     /locus_tag="PNS21_002192"
     CDS             complement(11495..12355)
                     /locus_tag="PNS21_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDI5159890.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLKQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            12447..12875
                     /locus_tag="PNS21_002193"
     CDS             12447..12875
                     /locus_tag="PNS21_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDI5159891.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13037..13213
                     /locus_tag="PNS21_002194"
     CDS             13037..13213
                     /locus_tag="PNS21_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDI5159892.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            13240..13686
                     /locus_tag="PNS21_002195"
     CDS             13240..13686
                     /locus_tag="PNS21_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDI5159893.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         4498 a         2349 c         3074 g         3826 t
ORIGIN      
        1 caatgtctca aatgatggac tttttgagca tcttcacaga tgggatggtt gctggtagtc
       61 gtatcttgaa gattatggat acagaagaat atgcaccaca acaaaaccct ggagcagatc
      121 aaatgattac ttcaggtaag attgagtttc gcaatgtaag cttttcttat gatggagagc
      181 acaatgtatt aaacaatatc tcttttgttg ctaatccggg agagaccgtt gcattagtgg
      241 gacacaccgg aagtgggaaa agctcgatca tcaatgtgtt gatgcggttt tacgaatttt
      301 atgaaggtca gatattgatt gatgatcatg acattcgaga atatccaatg gaagagttac
      361 gtaagaaaat ggggatggtc ttgcaagatg ctttcttgtt ttatggggat atcgcaggaa
      421 atattcggat gatgaatcca gctatcacag acgaacagat acgccatgcg gctgagtttg
      481 ttcaagcaaa caaattcatc gatgaattac caaaaggata tcactcaaaa gtgatcgaac
      541 atggtgccag ctattctagt ggacaacgac agctgatcac ttttgcgcga acaatcgtga
      601 ctgatcctaa aattctagta ttggatgaag caacagcaaa tatcgatact gaaacagaag
      661 gattgatcca agaaggattg gcgaaaatgc gacaaggacg gacaacgatt gcgattgccc
      721 atcggctgtc aacgattcga gatgcagaat tgattttagt tttagacaaa ggtcatatag
      781 tagaacacgg gaatcatgaa acacttctag cgcaaaatgg tttgtatgca gatatgtaca
      841 aactgcaaag cagtggtata taaaagatgt tgtgacagta gcattttgac ctaagcacta
      901 gagtgaaaat aaaacatagc ttcccatatt tttcatctgt gagagttagt gtcgtagtta
      961 ccagatttaa aaaagacgac gtcagctagt cagaagtgcc taatagcccc taagaaagcg
     1021 ttgacaaaat cgtttgcttt cgataataca aagaaaacga tacaacttgg atagtgattg
     1081 cctaatgcaa gctaaacctg atgagtctcc catggtttca tggacggctt tttgggtttt
     1141 tctttttgtt ttggatacag ccctatatat catggaaagg agcaaaacat gctgattgag
     1201 aaagtactga ataacaacgt cgtaataacc agagatgaaa gaaatcagga aatgatcgtg
     1261 atgggaaaag gtttggcatt caaaaagaaa gtgggtgacc cgatttttga ctcatgcatc
     1321 gacaaaaaat ttcagttgac aaatgacgga ctttcacaac agttccaaga attggcacaa
     1381 gcgattcctc ttgagtactt agaaatttct tgtgaaatca ttgaatatac aaaagaggat
     1441 ctacaagtcc atttgaatga ttctgtgtat attgcattga cggatcattt gtatacagct
     1501 atcgaacgtc aaaaaatggg gatctttgtg ccaaatgtat tgctgtatga tatcaaacaa
     1561 ctgtttccta aagagtatgc cattgggaaa aaaacagtga agaaaatctc ggataaatac
     1621 cacatcaagt tatcagaaga cgaagccgga tttatcgcat tgcatttagt caatgcacaa
     1681 ggcattcaaa gccagcaaga caatacctac ctgtttacca caacaataca ggatatttta
     1741 aatatcgtcc gctattacta taagattaca tttgatgaag attcaatcca tttttttcga
     1801 ttcgtcacac acctacgttt ttttctgaca cgcgtcctag atggagagaa atcagaagtg
     1861 ggagaagttt ctgaggaatt gttccaggtt attcaaaaaa aatataagga cacgtttagt
     1921 tgcattgaga aaatagcaga ctttttaacc ggtgctttcg actaccagat gtccacggat
     1981 gaaaaaatgt atttaacgat ccatactgcg cgtttagtgc atgagaatcg ttgtaaataa
     2041 ataaaaaagg aggacaaatt aacaagaacg tatgaaaata agggtagtga cattcagttg
     2101 gctaaacctt ccactaatta attggaggga atagaaatgg gaaaatacca ggaattagca
     2161 gaaaacatcg taaagaatat cggaggaaaa gaaaatatcc gtagcttgac acactgtgtg
     2221 acgcgacttc gtttcaaact aaaggaggaa aataaagcaa aggatgatgt tttgaaaaag
     2281 atggaaggga tagtcaccat catgaaaagc ggcgggcaat atcaagttgt aatcgggaat
     2341 catgtgccag atgtctatgc agaagtactg acagtagccg ggatttccga tgaagcagaa
     2401 gaagaggttt cttcaagtag taatctgttc aatcgattaa tcgacatatt aagtggctgt
     2461 ttccagcctt ttttaggtgc tttggctgca gcgggaatgg tcaaaggatt aaatgctttg
     2521 ttcttgtttc tcaaatttta tactgaagga tcgggtacgt acatcatgct gaatgggata
     2581 ggggatgcaa ttttttattt catgccagtg atcttagggt acacagcagc gaaaaaattt
     2641 catctgaatc cgatggtggg gctcgtgatt ggtgcagcac tttgttatcc gactgtacaa
     2701 ggagcagcac tacaaagtgg tttccctgat ggcagtcctg cgccttatag cttgctgggt
     2761 cttccggctt atgaaacatt tttgggtatc ccatgggtgg gcagcagtta taccagtagc
     2821 gtcgtacctg ttatcttcat tatcgcattt gccggacaag tccaaaaaat tgccaagaaa
     2881 tggattcctg cagtcgtcca gacctttttg gttccttttt ctgtgttatt aatcgcactt
     2941 cctgctggct ttttgattat cggtccgatt gtcagtatac tgacagatct attaagcacg
     3001 ggattccaag cattgatgga cttttctcca gctgtgtatg gtgcagttct agggtttctt
     3061 tggcaagtac tggtgatttt cggacttcat tggagtatta tccctcttgc gattatgcag
     3121 ctagcgcaaa atgggtggag tcagatgtta gtaggcgttt atggcgcgag ttttgcacag
     3181 acagcagtgg tattggcgat gtatttcaag ttcaaggatc aaaaaatgaa ggcgctttgc
     3241 atgccagctg tgatttcagg aatcttcggc gtaacagaaa cggctattta tggaatcacc
     3301 ttaccaaaga agaagccatt tatttactcg atgattggtg gtgctgtttc aggaatctat
     3361 ttgatggcat ctggaacaat cagctacaca atgggcgggt tagggatttt tggggtggta
     3421 aactacatca cggaaacaga cgccagtggt atgtttcaag cgtttatcgg cattgctatt
     3481 gcttcggtaa tcgggttttc gctgactttt ttcttttgga aggatgaagc aggtgaaaca
     3541 gatgatatac agaaaaaatc aggtaaagaa attgtaaaaa caccgataca aggacaaatc
     3601 gctccactaa atgcagcaaa ggatgcagct tttgcacaag gaacactagg tcgaggaatc
     3661 ttgatctacc ctgaaaaagg agaagtacgt gccccttttg acggaacagt catgaccctg
     3721 ttcccgacga aacatgcaat tggaatggtt tctgaaacag ggctggaatt gctgattcat
     3781 gtcggattgg atactgtgca gttggaaggg aaatattttg aaagcttagt gcagcaagga
     3841 gaaagagtga aacaaggcga tctgttgcta cgcttcgaca aaaagaaaat cgaaaaggaa
     3901 ggatacagtt tagaaacacc tgtgatcgtc accaattaca cggattattt ggatattttg
     3961 gaaaaccaag gggagacggt cgggccagac gatgcactta tcaccgcact gacctgaacg
     4021 gttaggtgaa gaatggacta ggcttaacta ttctttttat tccacttctt tattttcaaa
     4081 attgaaaggc tctttcatga tggctgagct gatagggcgt ttatcgaatt tttgaccgac
     4141 agaaattaga tggactaaat tctgttgata attttctgca aaaacacaac tatataacac
     4201 gtctagaaga taacaaattg atgtattgat cgtaaaatta ccaatttttg aatacagtcg
     4261 ctcgcgtgtc gtaagtcgca aagcggctgt actcattgca gatagcgttg aatcaccgat
     4321 actggtcaaa gctaaaattg gaacatcatt ttgtttcaat atttctgcgg tacgaaggat
     4381 cacattgttt tcacccgtat aagaaatcag aatcccgcac atatcactcg tgcaattgta
     4441 cgcttcatag aacgattcac caaaagtggt gacaatcgtg acatccattt tgatgcgctt
     4501 cattttcagc atgaaatctt gagaaatcaa agtattcgag ttactggcga aaatcacgat
     4561 tttttttgct tgaagcatta gttgtttggc tttttgcagt tggtcatgat gaatgagaga
     4621 aagtgtatca tgaatcgtca tctgttctaa agaagccatt ttatttgcga tcgtcataat
     4681 accgtctgtg gtggaaaaag gaagattcgc atctacgtct tgaaaattcc cttcaagata
     4741 ggctgtttca gctaaaaaag cattacgaaa actgacccag ccatcaaaac ccattttttt
     4801 tgctacacgg atgagtgtag acgggtggac atagcatcgc tcagcgatct cttggatggt
     4861 catttcgcca agtgctggag aatcgcttaa gagaaactgg atcacttcat tttctgaagg
     4921 agaaaaggag atttctttga ttttttcacg taataacatg tagttacctt cttcttattt
     4981 ttttacgtaa attataactt ttttgaaaat gttttgtaaa cggatttata ctatataact
     5041 taaaatagac tttttttgac aagatattta caaaaatcca ctagaaaaca gtaacttttg
     5101 taaatgttac tggaaagaaa cggatttcta ttgtttgttt tgtttacaaa aaagataatg
     5161 aaagtgtaaa aaagtaaagg agtgagaaca tggcaaaagg aatcaaaatc gctacgattg
     5221 gtggaggcag cagttacacc cctgagttga tggaaggatt tatcaaaaga tatgaggaat
     5281 taccgatacg agaaatctgg ctcgtagatg tagaagaagg aaaagaaaag cttgagatcg
     5341 ttagtcagat gtctcagcgt atgtgggatg cttctccgta tgaagtcaaa atctatgcaa
     5401 ctctggatcg ggaagaagca ttgaaagatg cggattttgt tacgactcag tttcgtgtcg
     5461 gtcaattaga tgcacgaatc aaagacgagc ggatccctag ttattatggg atgattgggc
     5521 aagaaacaaa tggtgctggc gggatattca aagcttttcg taccgttcca gtggtcttgg
     5581 caatcgttga agacatgaag cgtttatgtc caaatgcttg gctgatcaat ttcgcgaacc
     5641 caagtgggat ggtgacagaa gcagttatca gatatggcaa atgggataaa gtgattggat
     5701 tatgcaatgt gccagtaatg gcgatgatga cagaaccagc aatgcttggc aagaaaaaag
     5761 aagagctgat ctacaagttt gccggtctaa atcatttcca ttggcacaaa gtagcagata
     5821 ataaaggaaa tgaccgcacg gcagaattga ttgatcgttt atacgatgaa gacaatggat
     5881 tgccgaaaaa tatctttgat atcccgtttt tcaaagaaca attaaaacaa atgaatatga
     5941 tcccttgtgg ctaccaccgc tattactacc gtgaagaaga aatgctggaa catgctttag
     6001 aagaatatca aacgattggt acaagagcgc agcaagtgaa agaaacagaa gcagagctat
     6061 ttgaattata caaagatcca gaattagacc ataaacctga acaattgcaa aaacgaggtg
     6121 gcgcttatta ttcagacgct gcctgtgaaa cgattgcttc aatctatgga aataaagaaa
     6181 cacagatcgt ggtatctaca aagaatgatg gagctgttcc tgacctgccg gcagattgtg
     6241 tagtagaagt cactgcctat gttggcgcac aaggagcgag aaatgtagcg tttggttctc
     6301 ttcctgctgc tgaaagaggc tggttgcaag taatgaagaa tatggaatta ctgacgatcg
     6361 aagcagctgt gactggtgat tatgggacag ctttacaggc gtttacgatc aatccgctta
     6421 tacgatcagg aaagacagca aaacggatca tggatgagtt gtttattgca caccagcagt
     6481 atttaccgca attcaaggaa acaatcagcc gattaaaaga agaaggaatc gaagttcaag
     6541 atgaactggc aagagaatta tcaggtaata cacatataat ggaggaagta taatgggatt
     6601 cagaaaagat tttttatggg gcggtgctac agcagccaat caatgtgagg gcggttatga
     6661 tgaaggtgga cgcggactag caaacgtaga tgttgtgcca atcggaaaag accgttttcc
     6721 tgttatcacc ggaaagaaaa aaatgtttga atttgatgag gatcattttt atccagcaaa
     6781 aattgcaatc gatatgtatc atcgttataa agaagatatt gctttgtttg cagaaatggg
     6841 gttcaagact tatcgaatgt ccatcgcctg gagtcgtatt tttccaatgg gggatgaaaa
     6901 agagccgaat gaagctggct tgaaatttta tgaggatgtc tttaaagaat gccaaaagca
     6961 cggaatcgag ccacttgtga cgattacgca ttttgattgt ccaatgcatt tgatcaaaga
     7021 atacggtgcg tggcgttcca gagaactcgt tggtttttat gaaaatctgt gtcgagtcat
     7081 cttcaatcga tataaaggac ttgtcaaata ctggctgaca ttcaatgaga tcaatatgat
     7141 ccttcatgca ccattcatgg gagcaggact gtactttgaa gaaggagaaa acgaagaaca
     7201 agtcaaatat caagcggcac atcatgagtt ggttgcaagt gccatcgcaa cgaagattgc
     7261 tcatgaagta gatccggaga acaaagtagg ctgtatgctg gcagcaggag catattatcc
     7321 atacacttgc aatcctgaag atgtttgggc ttctagaaaa tcagatcgag aaaattattt
     7381 cttcattgat gttcagtcac gaggagaata cccagcttat gcattgaaag aaatggaacg
     7441 aaatggcatt cagatcgaaa tggaaccagg ggatgccgca ttgctgaaag aacatactgt
     7501 tgattttatc tctttttctt attattcttc ccgagtttcg actaccgaca acgaactctt
     7561 ggaacagact gcaggaaata tctttgcttc tgtcaaaaat ccatatctgc aagcaagtga
     7621 atgggggtgg caaattgatc cattaggctt gagaataacg atgaatgata tttatgatcg
     7681 ttatcaaaaa ccattattta ttgtagaaaa cggcttaggt gcagtcgata cgccagatga
     7741 gaatggttat gtggaagatg attaccgaat cgattacctg gcagcgcata tccaggcgat
     7801 gaaggatgca gtagaactag acggagtcga tctattaggc tatacgactt ggggatgtat
     7861 tgatttagta tcagctggta ccggtgagat gaagaaaaga tacggattca tttatgtcga
     7921 tcgtgataac caaggaaatg gcacattagc ccgttcaaag aaaaaatcat tcaactggta
     7981 taaaaaagta attgattcaa atggagaaaa tctttcgaac gattgatcaa tgatttataa
     8041 aagtctattg tttttcagcg gatttttcga gtataataaa gctattcgag aaacgcaagc
     8101 agaacaatag actttttttc gcattggctg taaagtatgc ttacttgatt aagtaaagga
     8161 gaatctacat atgtatgatg taatcgttat tggcgcaggt cctgctggga tgactgctgc
     8221 tttatatgct tcacgttcaa acttatccgt attgatgatc gaacaaggtg cccctggcgg
     8281 ccaaatgaac aatacggcag aaatcgaaaa ttatcctgga ttcgattcga tcatgggccc
     8341 cgatctagct tataagatgt atgaaagtgt ctcaagattt gggacagaaa atgcttatgg
     8401 aatcgttcaa gatatcaagg atcacggcag ccataaagaa gtcatctgtg aagataaaag
     8461 ttacgaagga aaaacagtca ttatcgcaac aggctgtatc caccgtaaat taggcgtacc
     8521 tggcgaggaa gaatttgctg gacgaggcgt ttcttactgt gcagtctgtg atggtgcgtt
     8581 tttccgtaac aaacgtctag tagtcatcgg gggtggggac tcagcagtag aagaagccat
     8641 ctatttgaca caatttgctt ctgaagtagt gatcgtccac cgtagagatg aattacgtgc
     8701 acaaaagatc atccaagacc gtgcatttgc aaatgaaaaa atttccttta tgtgggatac
     8761 agtcgttgac gaaatcgttg gaaacgaaat ggtagtgaca ggtgtgaaag cacgtaatgt
     8821 caaaaccggc aatgaatttg atttagcagc agacggagta ttcatctatg ttggactgga
     8881 tccattaact gcacctttta gagaagcagg tttgacgaac gaagctggct ggattccaac
     8941 agacaacgaa atgaaaacga atatgccagg cgtttttgcc atcggagatg ttcgtgaaaa
     9001 agatttgcga caaatcacta cagctgttgg tgacggcggt attgctggac aacaagtgta
     9061 taaatacttg gaatctttag aagaaacaac tgcataagtt taaaaagagt agaagaacgg
     9121 gttcaattgt tcttttactc ttttttttcg cgtgaaattt actgaatctg atttatgcga
     9181 acacgtccaa caccttttga taaaagaatc ctttcttgat ttaagcgttt tttggtcctc
     9241 atactttcat atctaataag tgaatgctat acttattaag aaccatattt cagaaagagg
     9301 tgttattttc atgtcttggg aacaagtgta tgaacaatgg gtacatgaag aaaatttaga
     9361 agaaaactta aaaaaacaat taaatgatct gggtgaagat cctgaaaaac ttgaagatgc
     9421 tttttatgca ccgttggaat tcggtactgc aggcatgcgg ggaatcttag gacctgggat
     9481 caatcgaatg aacatctata cggtaagaca agcaacagaa ggactagcac gtttcatgga
     9541 tacacaaggt gaagaagcaa aacgccgagg tgtcgctatt gcttatgact cacgccatca
     9601 gtcaccagaa tttgcaatgg aagcagccaa aacactggca catcacaata tcccgtctta
     9661 tgtattcgaa agtctgcgtc caacaccaga gctttctttt gcagttcgtc atttgaagac
     9721 gtttgcggga atcatggtaa ctgcatctca caacccagcg gcttacaatg gatataaagt
     9781 ctacggagaa gatggcggac aaatgcctcc tgcagacgca gatgcattga cgaaatatgt
     9841 ccgtgaagtc agcaatccgc taaaagtcga agtattatct gacgaagaag ctaaacatag
     9901 cgggttgatc acgatcatcg gagaagaagt ggacgctgct tatctagaag aaatcaaagc
     9961 agtgacgatt gatcgtgaat tggtagaaac aatggggaaa gatttgaaac ttgtgtacac
    10021 gccacttcat ggaacaggga aaatgttggg cgaacgtgcg ttgaaacaag ctggattcga
    10081 acaattcgta ctagttcccg aacaagctgt ggcagatcca gatttcacta cggtcaaatc
    10141 gccaaatcca gaagaacatt cagcttttga atatgctata cgacttggag aaaaagaagg
    10201 agctgacttg ctgattgcaa cagatcctga tgcagaccgt ttaggcgcag cggttcgttt
    10261 gccagatggt tcttatgaag tattgactgg gaaccaaatc ggggcattgt ttgctcaata
    10321 tattttggaa gcacatcaat caagaggcac tttaccagaa aacgcagcgg tattgaaatc
    10381 aatcgtttcc agcgaattgc ctacagcaat tgcggaacac tacggtgcta ccatgttcaa
    10441 tgtgttgaca gggtttaaat tcattgctga aaaaatccaa caattcgaag aagaccattc
    10501 acatactttt atgtttggat tcgaagaaag ttatggttat ttagttaaac cttttgttcg
    10561 cgataaagac gcgatccaag cattgctttt attagcagaa gtagcagctt actataaaaa
    10621 acaaggcaaa acgttgtatg acggcttgca aacgattttt gaaacctatg gctattttgc
    10681 agaaaaaacg atttctgtga caatgagcgg acaagaaggt tctgcgaaaa tctctgcttt
    10741 gatggaaaaa ttccgtaaag aagcaccgac agaatttgcc ggagcaaaag ttattcaaac
    10801 agaagacttt aaagaattga cgagaacaga ccgtgaagga aacgtagaaa aactaacaac
    10861 accgccatcc gacgtattga aatatacatt ggaagaaaat ggctggattg ctatccgccc
    10921 ttcaggaact gagccgaaaa tcaagtttta tattggtgta aaaggaagct ctacagaaga
    10981 cgcagccgaa aaagtagcct cttatgaagc gattatcaaa aagatgacag aagaataaaa
    11041 aacttgagac tgatggaaaa agtggtaaac tttagaagtc agtctttttt caagttggag
    11101 gtgaatggat tgccagacac gcaaaaatca aaagtagaga aagtcagtca attattcaaa
    11161 atgttgagtg atcccactcg tcttaaaatt ctgctatatc tgaaagacgg ggaacaaaat
    11221 gtgacagcaa tcacccaagc agtggaaatg gagcagtctg ctgtatcgca tcaactacgt
    11281 ttattaagag aaaatcatgt cgtcaagtct catcgcgagg gaaaagcgat tttgtatagt
    11341 cttgatgatt ctcatgtact ggatattttg aaccagacac tcaaacatgt ggaacatcaa
    11401 taacgatagg tgcaaataga taaacactgg agtgtgacaa cgaaatttct tcgttgtcac
    11461 actccagtgt tttttaagaa aaagacaaaa atgattaatc gcttttttct gttgtttcag
    11521 ttccataata actatgatct tctaggtcta aagcattcaa aataagcgaa gccgtttctt
    11581 caatagataa agatgccaca ttgatcacta cacatcctag tttttcataa agatcattcg
    11641 caaaatcgag ttcagctcta attttttcga tatcggaata agcagtatca gggtttaatc
    11701 cgtaagagcg catccgttct ttacggatat tatttaaaat atctgggtta ttcgttagtc
    11761 ccacgatttt tttaggatcg atctcccaaa gctgtttggg aatatgtgct tgtgggatca
    11821 aaggtaaatt ggcaactttt aaatttttgt tagctaaaaa caagctcaaa ggcgttttgg
    11881 aagttcggga tacgcctagc aaaacgacat ctgcttctaa aaaaccacga ggatctttcc
    11941 catcatcgta ttttacagca aattccattg ctttgatccg tttgaaatag ttctcattca
    12001 ataaatggag ggcaccaggt tgccgaattg gcttgatacc ggttaaacgt tcaatttctg
    12061 atatcggtgg cgtcatcaca tcaaaactaa ataattgatg ttcttcacaa aaagaacgtg
    12121 ttatctcgat cagttcttga ttgattaatg tatgcaggac aagtgctttt tcttctaatg
    12181 catcttgcaa tgcctgcttt aatgtttttt catctgtcac gaacgttctg cggaataggt
    12241 tgaactcaac ggtaggatac tgagccatag tagcttgcgc tagttttgtc gcagtttctc
    12301 cagcagagtc ggagatgaca aaaaaagtaa cgatttctgt agaatcggaa gacatggata
    12361 aaacctcact tgcatttttt ttatgtttat tataccatac tctagaatgc gtacttcgta
    12421 atcattttga tttggaggaa taagtcatga aaaaacagac attgatcgaa caatcattag
    12481 aaacattgaa aaataacggt tttaaatata ctaaaaaaag agaagcattg ttaacttatt
    12541 tagtgaagcg taaccgctac gtatctgcta aagaagtttt tgactatatg aatcaggaat
    12601 tcaaaggggt aagttatgat acgatttatc ggaatctcca tgattttgca gagatcgatt
    12661 tactggaaga aaccgattta aatggagaaa tgaagtttcg ttttcattgc tgtcaggggg
    12721 aaatcggcca tcaccatcat tttatctgta cagtgtgcgg aacaacgaaa gaaatccata
    12781 tgtgtccgat gaattatttc gaagaacagc taggtggatg cacgatcgaa gggcatagat
    12841 tcgagatttt cggaagatgt gaaaattgtc aataagggtt taaataaaat aacatttgat
    12901 aattttaaaa aaaagcaaga tttccttgac ggtcaaaaca gcattagata taatatttac
    12961 aggacagtct tgttttttga gatatatttc aataacaatt acggttactt tttaagctcg
    13021 gagggaggga attaacatgt caaaaacagt cgttcgcaaa aacgaatcac ttgacgatgc
    13081 tcttcgtcgc ttcaaacgtt ccgtgtcaaa agcgggtact ttacaagaat ctcgtaaacg
    13141 tgaattctac gaaaaaccaa gtgtaagacg caagaaaaaa tctgaagcag ctagaaaacg
    13201 caaaaaattc tagttctgtg atgaattgga gttggtagtg tgtcactact aagtagatta
    13261 aacgatgaca tgaaagcagc gatgaaagcg aaagataagg aatctttaca agtcatccga
    13321 atgatcaagt catctataca aaacgagcag attaaagaag gtcacgactt gacagaagaa
    13381 gaagaactaa cggtgttgtc tcgcgaaatg aaacagcgac gtgattcttt gcatgagttt
    13441 gaagaagcag gacgtgacga tttagctgaa aaagtaaaaa gtgaaattgt aatagtcgaa
    13501 aaatatatgc cggaacaact ttccgatgaa gaaattcgac aacttgttca agaagcaatc
    13561 actcaaactg gtgcttcatc aatgaaagaa tttggcaaag ttatgggtgc aattatgcct
    13621 aaagtcaaag gaaaagcaga tggaaaccaa gtgaatgcga ttgtgaaaga actattacaa
    13681 tcttaatttt atcatttaaa ctgcggacag tgattgtccg cagttttttg tgttcgtaaa
    13741 aaaagaa
//
LOCUS       DAMYXM010000066        13309 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.067, whole genome shotgun sequence.
ACCESSION   DAMYXM010000066 DAMYXM010000000
VERSION     DAMYXM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.067"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            137..310
                     /locus_tag="PNS21_002196"
     CDS             137..310
                     /locus_tag="PNS21_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159894.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            950..1423
                     /locus_tag="PNS21_002197"
     CDS             950..1423
                     /locus_tag="PNS21_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDI5159895.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1432..1659
                     /locus_tag="PNS21_002198"
     CDS             1432..1659
                     /locus_tag="PNS21_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI5159896.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2295..2666
                     /locus_tag="PNS21_002199"
     CDS             2295..2666
                     /locus_tag="PNS21_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159897.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2922..3164)
                     /locus_tag="PNS21_002200"
     CDS             complement(2922..3164)
                     /locus_tag="PNS21_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDI5159898.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3196..4098)
                     /locus_tag="PNS21_002201"
     CDS             complement(3196..4098)
                     /locus_tag="PNS21_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDI5159899.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4111..4299)
                     /locus_tag="PNS21_002202"
     CDS             complement(4111..4299)
                     /locus_tag="PNS21_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDI5159900.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4313..4876)
                     /gene="amaP"
                     /locus_tag="PNS21_002203"
     CDS             complement(4313..4876)
                     /gene="amaP"
                     /locus_tag="PNS21_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDI5159901.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4914..5807)
                     /locus_tag="PNS21_002204"
     CDS             complement(4914..5807)
                     /locus_tag="PNS21_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI5159902.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5885..6808)
                     /locus_tag="PNS21_002205"
     CDS             complement(5885..6808)
                     /locus_tag="PNS21_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDI5159903.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6857..7207)
                     /locus_tag="PNS21_002206"
     CDS             complement(6857..7207)
                     /locus_tag="PNS21_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDI5159904.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7240..8142)
                     /locus_tag="PNS21_002207"
     CDS             complement(7240..8142)
                     /locus_tag="PNS21_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDI5159905.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8135..8992)
                     /locus_tag="PNS21_002208"
     CDS             complement(8135..8992)
                     /locus_tag="PNS21_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDI5159906.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9326..10135
                     /locus_tag="PNS21_002209"
     CDS             9326..10135
                     /locus_tag="PNS21_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDI5159907.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10175..10672
                     /locus_tag="PNS21_002210"
     CDS             10175..10672
                     /locus_tag="PNS21_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDI5159908.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11057..11130)
                     /locus_tag="PNS21_002211"
     tRNA            complement(11057..11130)
                     /locus_tag="PNS21_002211"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11094..11096),aa:Ile,seq:cat)
     gene            11317..11640
                     /locus_tag="PNS21_002212"
     CDS             11317..11640
                     /locus_tag="PNS21_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159909.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11804..12058
                     /locus_tag="PNS21_002213"
     CDS             11804..12058
                     /locus_tag="PNS21_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159910.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12128..12373
                     /locus_tag="PNS21_002214"
     CDS             12128..12373
                     /locus_tag="PNS21_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI5159911.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12449..12814
                     /locus_tag="PNS21_002215"
     CDS             12449..12814
                     /locus_tag="PNS21_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159912.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
BASE COUNT         3869 a         2460 c         2235 g         4745 t
ORIGIN      
        1 tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc gttcttactt
       61 tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc ttataaataa
      121 aaacgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa cgattggcta
      181 tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat tcagttggac
      241 tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac aaaattatcg
      301 aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta tggtactttt
      361 tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac ttttgattta
      421 taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga tagtaccacc
      481 ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata aaggacaaat
      541 aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa taaacatgaa
      601 cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct catacagttc
      661 tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta ttgtccaata
      721 taatcccagg catctaaact agcattatag aagagacaca catagaatct aaaataactg
      781 gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac cttcatactc
      841 tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag ttgttttaca
      901 ataattattc tgctatattt tagctatcaa agataaaaga gggataaaaa tgaaatttaa
      961 aacattgcta ttaaaatttg tcgttttagg tataagtgcc gtatttattt tgttcggcgt
     1021 acttttttat actcagatcg ctacaagcga aaaaggattc aattttgatt ggcagaccac
     1081 attatttgtt tctgtcattt actttgccct tctcctaggg atcgttattg cttatttctt
     1141 attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa gcttaaacat
     1201 actaaaaaaa atcgaacgaa ccattttgtt tattggtttc gccttttttg gcttacttcc
     1261 taaatttatg aaagtagcgg atttagacga tgctccaggt ttgatgttgg taggaatcgc
     1321 tgtcgtattc attccttttg cagtatacac attaacaagc gtgctggaaa aactgcttga
     1381 acatgcgatt ttacttaaga cagatcatga tttgacagtg tgaggtgcga catggcaatt
     1441 atcgttaatt tagatgtagt attagctcaa aaaaaaatga gtgtgactga attgagtgag
     1501 aaagtgggca ttacgatggc aaacatctct attctaaaaa atggaaaagc aaaagccatc
     1561 cgtttttcca ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg tgatttgttg
     1621 acctataaaa atgactcgaa tactatacca gaagaataaa ataaagtaga acaaacagta
     1681 aataaaaaat aatctgaatg atagatgtgt gagactttta gaagttattt acgtctatta
     1741 ttcagatttt ttatttcgat tgaataccgt tttatctata aataagatat gtactgactc
     1801 atacaaaggg ggccttttct tctccatgta ctatcacttt tcctgataca actggtttcc
     1861 tgttacgttg tagttggatc tgcttttagt ataatacttt attcattcca ccagactgat
     1921 agcagtttga gcagtctaaa taatcatgta gtattacagg ccattctgtt tcataccttg
     1981 aagtgcttaa aacttcagct aatggcccat tgtgttaact cttactccat tcttttttag
     2041 taagctccca tatatactgt aaagccttct atatattatg tcatgttgct ttttaacatt
     2101 tactatgtag ttttgataaa atgatttgaa taacagtgat ggacaattct tttttatttt
     2161 taacattatt ttgataataa tgattattta tttaaaaata cattagataa catttgttac
     2221 gacacgaaaa agcttcacaa acaaatatcg ttattttaaa atgagggcga actcacacaa
     2281 ttaggaggaa tagtatgcca tttattgttt acttttttct ttcattactc gtgatctata
     2341 ttccattacc aacatttttc ctgctcgtca atcgtctagc agagcaacat gatacgattg
     2401 ctgctcttac gaaactttgt ttacttctat ttgtttgtct agattacttt acgagctatt
     2461 attggctaaa acagacaaga gtaaaaaaga gatattattt cttccttgtc tttttcaatc
     2521 tctttgagtt tctgatccat tgctttttac ttcatcagta tcatacgatc catttgtgga
     2581 tcatctgggt tttcaaagtg gtcgtattat tttgtatgtt cacctttcct atctcaagaa
     2641 aatttaggga ggcattgttt tattaaagaa gcttctctgt atcaataaag acacatttaa
     2701 ggaaaaactt ttaaaaatct aattgggaat ttgcccgcat aaataagagg ctgtgacaaa
     2761 agtcttgtca cagcctctta ttccgtataa acagtgttca aaagcttgca tcttcgataa
     2821 tatgcccaac caaatgaaaa atatgagaag ctatttttgt ttggctgttc ttattacttg
     2881 atgaagaaca gctttttcac gacctctttt taaaagtctt tttacagttt tcgagcaaag
     2941 aatgatacga ttagtactaa aatgatggca ccaatcaatg atggcacgat tgccattcct
     3001 gcaatacttg gtccccaaga acctaataat gcttgaccaa gtgctgatcc aactaaacct
     3061 gctacgacat ttgcaataat ccccatcgaa gaacctttat tcgtgattgc tcctgcaatt
     3121 gctccgataa ttcctccgac gataagtgac caaataaatc ccatgtttcg ttcctcctaa
     3181 tgttttactt ccttattact gaacccgtgg ttctgtattt tcttttactt tttgtgcgcc
     3241 gcttccagct gctgatttta ctttatctgt ttgattcgaa gcaaattcac cggttttttt
     3301 cgctgcgtca gttactttgt cttgcaccgt ttcttgatct ttttcaaatt cttcttttgt
     3361 tttgatgtcc actacgttag cgttgacttc gatcacttct aaatgtgtca tattttttac
     3421 ttctttgccg attacttctt gcatttcttc aaagattttt tcgatatctt tgccatattc
     3481 cacaatgaca tccatatcaa ctgctacttg ttttttaccg acttctgttt cgatcccagc
     3541 cgttacatta tctgtattca caagttttcc tgccacatta gagaagaaac caccgtttgc
     3601 agataataaa ccgtctactt gttcaattgc gattccgatg attttttgca caactttatc
     3661 ttcataattc agctcaccgc gcattgaatc ttcctgttca gaatcatgct ttccttggtt
     3721 atttgtatga tcagaaccaa gttttccttg tccgtttgtg tggtcagaac taagtttttc
     3781 ttgtgctttc gcatggtcag aaggatgttt ttgctcttcc acaggtttag aaggatcgat
     3841 tgcatcatgt tgttctggaa ctttagattc tggagttcct ggatgatgag gtgcatgctt
     3901 ttctgcatca tgtttcttct cttctactcc ttgttgaacg ttagggacta ctggatctgg
     3961 atgtgcagat actggtgctg ttttgttctt ttcatcgttc acaccatttg attggtttgg
     4021 tgtcgttggg tctggatgtg cagacactgg tttttggttg ttcattttat tgttattatt
     4081 tttgttattg ttattcatgg atgtttcctc ctaagattta aaatagtgat ctaagatttt
     4141 cgtttgtttc agataaaaac cgatagcgac gccgatcgct gccatgatca agatcaataa
     4201 ggtcttgaaa aagcccacgg tgatccataa aacggctaat atcaatccaa gtaccccaaa
     4261 gatgataggg agcttgtatg tcgaaaatag ttctttcatt tttggtcccc ccctattcta
     4321 ctcgtttgcg gtcattgctt cgtttagctg gttcttcaaa gcctgtgtat ttcaccgata
     4381 ctttcactgg ctcttttgtt cccaatatgt gttgtacttc attttcgatt tctttcatca
     4441 atgttcctgt tttaccaatg agtgctgaag ttcgtttcaa ctcccctttt acattgacgg
     4501 cgatcttgtt tttcgttgct ttgacattta cttttggtga atggatgaat tcatcttcat
     4561 gcaagtgaga acgaaccatt ccttcaattg cttttttatc caacgtcaac tccccatcac
     4621 catgtttcag tagaaagttt ccgattgttt ttggataaaa caggaaaaca agtaacgtga
     4681 taattgtcaa aatagcaaat gcaacggcaa gccaaaatac atattgttgc acgtatgaat
     4741 tagtgatgga aaagaatcga aacttctcta gctgaaaagg caatggatag gttgcactta
     4801 atagtatcgc aaacaaaagc acagatagca aaagtaatgt tccgatcact ccgatcgttt
     4861 ttacaccttt attcattttc gcacctcctc ataggggtga aacgatcaac ttcttataag
     4921 ttgatcgctt ctccaccagt tactccgtaa atttgggctg taacataact tgctttattc
     4981 gatgctaaga aaacataaac tggtgctaat tctcctggct gacctgcacg ttctagtaaa
     5041 ctttgctgac caaattcagg gatcgaatca gaaggttgtc cttcgtctag ttgtaatggt
     5101 gtccagattg gaccaggtgc tacaccattt acacggattc ctttaggtgc aagctgtttt
     5161 gctaaaccga tcgtaaagtt agcgattgaa gcttttgttg ctgcgtaatc taataaatgt
     5221 tcgcttggat tgaatgcttg aacagaagtt gttgttacga ttgctcctcc tgctgggata
     5281 tgcggaacag cttctttcac taaagcaaac atagcgatga tgtttatcat aaatgtgtct
     5341 ttgacttgct caagtggtaa ctcagcaata gactgacaag aaatttgctg tcctgcgttt
     5401 agtaccatcg tatctagccc gccaagttca ctcacggctt tatctatgat ttctttaggt
     5461 gcttgttcgt cacgaagatc aaaaggtagt aagacaatct tacgtcctgc atctttcacg
     5521 tattttgcta cttcctctgc atctttttct tctcctggga agaattgaag agcgatatct
     5581 gccccttctc tagcaaatgc aatcgctgct gcacgaccga tccctgaatc tccaccagtg
     5641 attagtacac ggcggttttc tagttgatta ttgccttcat aggattcttc tccacaatca
     5701 ggaacaggaa tcatattctg ctgtaaagct ggtgtatcct gatcttgttt aggaaatttc
     5761 tccgtatggt acaagtttcg cgggtcgatt aattttggtt ctgtcattgc tgtattcctc
     5821 ctagttttta aattgtattg ccaaaacaaa tttctttgtt tgatacctgt tttaaataga
     5881 ttctttaatt tgtataatct ttccggtata aatggtaacc taaaactact gctgctatca
     5941 aagagccaaa gactacgagt ataaagccta gagcagaact tttacccggt aatcctcctg
     6001 tattcattcc ccaaatccct gcgaacaacg ccggaacgga aatgattaat gcagctgaat
     6061 ctagatattt catgatatga tttagattat tgtccatcat cccgttgaat agatcaccaa
     6121 tactctctaa taaatctcga tagatctcga tcattttttc tgtttgtcgc tgtctcaaat
     6181 ggacatcata gagtagctgt tcatcgtcta aacgtttgat gaaagtttca gaattccata
     6241 attgatccat tgtttcccgt tgatctctta aggtatgatc gatatatacg atgtctcttt
     6301 caagatctgc ctgtttatac agttcttcat tttccgttgt tttacgagct gctttatcca
     6361 aagcgtcaat tcgctttttc atctctctta gttctttcac gtaatgggtg taaatatcta
     6421 ataatgtata cgtgatgatc ttttcaaacg agtttaaatg atttccatgc tttgtcagca
     6481 gccgatccac aaattcggtt tttctatcga tacaagtaat caagagatta tcggaaagta
     6541 caaaagatat tggcgttaat tttttttcga tacgattatc tgactgatct agattaatca
     6601 ctaccaaaga aaatggatgc tctagtttcg tctcataaag gcgctccatc cgagaaactt
     6661 cttccggata ttcggttcct acgaaaatat catcaggaag ctcgaattct tcgattgctt
     6721 gctcaatctc ttcgggcttt gcactatcaa gtacgagcca attatatttt ttttcatctg
     6781 tttctattac attcttttct tcatccacaa agtagtatct caatgtatac ctcctttttg
     6841 gactttctcg aaactattag ctgaagaagc agttctttaa tttgatatta taatcgagta
     6901 ctgcgatttc attttctaat gaaaagctgt caagctgttt gttgaagtcc atgttcaatc
     6961 gtttaccgaa ataattttgc tctaatttag atgtatagat gtccaataat ggatgctcga
     7021 ttttagtcgc atattctcca aattccgaaa caaaaggaat gatttgaaaa aagctgcctt
     7081 ttgcaaaagc acctttgtcc cttagaaatg gatcggaatc attgataagc gcgagtgcga
     7141 tttttgtatt ttccccaacc ttttcaatta acgtctctac tgcattttta gacattgtaa
     7201 tttccattcc tactccccct cttcagttgt tgtcttctat tatttaacaa ctacatctgt
     7261 ttttggttga acttttgcat attttggata tttagggaat ttagctacac aacggaaagc
     7321 cgtcatccct gcatattttt cgtaatattt cttcgttggc ccttcaaaat ccttgctcca
     7381 cggtttcaca tgtcctgaaa aatggataat gtaaggattg ttgcgagttt cttcgtattc
     7441 acgttcacct gctgctgttg gatgtttttt tgcttttgcc atgatatagc cttgtgcatt
     7501 ccaacgcgga tgaagtggta accaacggtc atgtaagata gcatttaatg catcttggtc
     7561 atggaatcgt aatttttctg gattatgttc gatgaaatct aatacttttt gcgtaatatt
     7621 ttcatctaac cattttttca cattgatcag catcaaacct gagttgaaat aacgcattga
     7681 tttagctgaa attttcattt tttccagtcg ttgatggaac ccagcgtctt caacagctgc
     7741 tacgattgaa tcaccgaagt caagacgcca taatttagag atatcttgta aagcgatcat
     7801 gtcgcaatcc atatataaga tacgttcaac ttctgtgccg cggaacaatt ccggaatcgc
     7861 aatacgataa taagctgttt ctggaatacg atcgctgatt accacatttg taaagaattc
     7921 tttattgatt ttcaagaatt ccacatctga gttcattcgt gcgtttttca ctgagaagcg
     7981 taaaagctgt ttactttcaa aatcgatatc atcgtcaatc acatagaacc gaacaaattt
     8041 agacgggttg caattatcta atactgagac aaataatgca gctaaatgtg gtacgaattt
     8101 tgtgttacaa cttgcgacta ctgctatttc ttttttattc attgatctcg cctactttcg
     8161 ttagagtact gtgtgctaat ttttttaagt ataaattggt atacggatga tctttcaacg
     8221 tattccaagg tttatcatgt cctgtaaagt gaacgatcga aggtttttca tttccttctt
     8281 tatactgacg ttcatatttt tcatttggtg ctggatgacg ttcaaaaatc agcgaagtct
     8341 gcatgttcca ttttggatga agttgttccc aatcttcata aagtacagca ttcaaagcat
     8401 cttggtcgtg atagatgatt cgatcgccat tttcactgag gtaatggata gttttttctg
     8461 taatttcttt tttattccat tgatcaatgt caatcaccat cactcctgaa ttgaagtaca
     8521 agtcatcgga atcgattcct aatctttcca aagctttcac ttggcctgga tcgataatag
     8581 cgccgattgt ttttccattt aacggctcat catatagttt tacgatatcg tctagtacta
     8641 acacatctga atctaagtaa aggactttct tataatcctc tttagctaat aagtttggta
     8701 aagaaattct taagtaagcg gtagttgtga tatggtcact gactaggaag tcttcgtaga
     8761 tatctttttc cactgtcagg aattggatac ttgcattact tgaatattta tttaccgttt
     8821 cttccaatcc ttctttacta tattctgata gaccatcgtc aatcacataa aattttatgc
     8881 gtcttgtttt attgcaattt tctaatgcag ttgcgatcat cacgctcaag taaggagcat
     8941 aattttcgtc tgaagcagtt accacaggaa ctacgccgta tctcttctcc atcaaaatca
     9001 ctcctttttc taatcttttt ctaacctgtt ctacatctag aatattcctc atttggtttt
     9061 aaagcaaaat atatgctttg ttttcattga atattttaaa atttttttat cacaaaacgc
     9121 atcggtaagc gctaactatt cgtttttcat ggctttttat tcaaaggtat tgaccaaaaa
     9181 ctactatata tagccacctt tttcttaaga atgttttttg aaaaaattca taatgacaaa
     9241 gcaagttagt ttcatttttt taaattctca tttaatctta aggtaacagg aaaatagata
     9301 tgtaaaaaga aagtaggaag ataaaatgaa actagcagcg attgatttag acggtacctt
     9361 gcttgatcga aatggaaaga taccagaaga aaatatccaa gcacttcgga aatttgatga
     9421 aaatggcgga atcgtaaccg tcgctaccgg aaggaacagc atttctgcta aagatatttt
     9481 ttccgaatta ggaatcggtg gctatttgat ttcttccaat ggttcactga ttgctgaaat
     9541 gaaggatgga aaaatggcga aagttctcag acgttcaaaa atagatagcg atacactgag
     9601 aaaagccttc ttctatgctc aagaagcgaa aatatccatc attgctagcc gagaaaccca
     9661 agatgatcag ctgacattcg atgaatcaaa tctagtcaaa gatgaccctt attatgaaca
     9721 tttcaattta cagaaccatt cttttgatca aattttaaat atgttggaag atccttctct
     9781 tagctatctt aaattagcat taacagataa gagtgaagaa aaactacaga agctacaaag
     9841 agatctgcaa gcagatggta tccaatcagt attctctgat ccgcatttct tagaaatcac
     9901 gcctacaggt atcactaaag ctcattcact tgtttttctg gcggattacc ttggaatcga
     9961 atcgagtgac atcatggctt ttggcgatca agaaaacgac attgctatgt tgaattacag
    10021 cgggttgagt attgcgatgg ggaatgcaca gcgacaagta aaagaaattg caaaccaagt
    10081 aacactgacg aatgaagaag ctggtgttgc ttatatttta aaaaacttct attaaaaaga
    10141 ataaaatgac gatatgaatg taggaggaaa gaaaatgtta gttattattg atatgcaaaa
    10201 tcatattctt gatccaaaca gcgaatttta tattccagag tcagatcaat tggcagaacg
    10261 tattgctcaa agattagcta aagcacgcca aaataatgaa tatattctct tcacacggga
    10321 tataccagtt gaactgaagg acaaagaaga agaacgagaa gatttgcaat tgatccctct
    10381 tctttctcca caagaaaatg aacgagtgat taaaaagtat tactttacga ttccacctga
    10441 aactttagct gaaatccagg aaactttatt tgaaagcaaa gaagaacaaa aagagatcga
    10501 agtagtcggg attgaaacaa acttgtgtgt actctctaat ctgataggcc tgcagagtgc
    10561 ttttcctgaa gcagactttt ttgttgatcc aatgttagtc tccagtagaa aacacggaga
    10621 atccgcttta gagctgttga aagatttcaa cgtctctatc gtcgaatcat aaaaatatct
    10681 atcttcattt acgatattta acacacaata aaaaaagcac gcagaactgt tttagtccta
    10741 cgtgcttttt ctatcaaagt agattggctt ttttatgaca cagagtgaaa cggcaggctc
    10801 acgacctctt tcttaaatgg tgttcaatca gctgaccctc ggtattagct taacaaacgg
    10861 caaaatatga ggaactattt ttccgtttgt taagctaata ctcaggtcaa aacgctgatt
    10921 tcacaacctc ttccttaaac ggtattcaaa agcttgcatc tgcagtaata agaacagcca
    10981 agtaaaaaca taagaagctg tttttacttg gctgttctta ttacttgatg cagggcagct
    11041 ttttcacaac ctcttatgta ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
    11101 gtctgctcta accaactgag ctaaaggtac atcataaata aaatttattt ttgttacttt
    11161 tcaactatag cacgaatgac aaactctcac aagtttcaaa atataagtaa aataatattg
    11221 ttattcttat ttcttttact tgtgtcataa ctattttgtt actttaattt ttatttgctt
    11281 attttagaaa aaatgttatt cttttattta ttctcaatgt taggagtgat tattttgcca
    11341 tttattgcga ttttattcga tttagttgcg gcagcagctt attttctcca attaaattat
    11401 caaacctcag gaattctgtt tattggaatg atttttcaag gtgtgattac ccttttgctt
    11461 cttgtcatga tgctcagcta taaaggaaaa agatatgccc gtattcaaac aaaagtattt
    11521 gttaaatatg tttccattcg ctatgggatc atcattcttt cctttttagt gaatgcgatc
    11581 gtcctctttt tatatgtatt gaattactta gatatcaatc cattgatttt ttctcgatag
    11641 ataagacaca ttcagcggaa cagttcctgc ttatcagcag aaaactgttc tgctttttca
    11701 tttttcattg atctattctt cttttatttt cctagcattt catttgtgat gtatgcaata
    11761 acatgttatc ttttattata tgaatcgttg aggagtccgt tacatgatta cgttggtgag
    11821 agttttgttt tgggtgcctg cggtcgcact ggtcgcaagt attgtttatc ttatgaattg
    11881 gaacaaagaa cggttttatt tagctatttt gactcttccg gctatctact ttatgtggaa
    11941 agtttttaat tacaactact ttgaacccga tagtgttttt atagaagaat tatcaggact
    12001 ggttttgtct ttattgatcg tcattcttta tttgatacgt ttaaataaaa aacattgagt
    12061 actattgatt gcatgtgtag ctttctatag tcattataac tattctacta taatgaaagg
    12121 tgattaaatg agtatcgaag aagctatttt actggatact ttacgttgtc gtctgaattt
    12181 cttaagaact ggccgatctt tatctcaagt agctgtttct agcgcgttaa gtattccttt
    12241 attcaaatat cgtaagtacg aagaaggaga agaaattcca agcatcattg aattaatcta
    12301 tatcgctgac ttttacgata tttccgttga ttacctaatt ggtcgctcag aaacagcaag
    12361 caaactgaag tgattttttc cagccttggt gctggttttt tcatttaacg tctaaggtta
    12421 acaatctata tataaaggag acatttctat gcgattatgg catgaaaaat tgatttcaaa
    12481 gcttcctcgt caacagcttt taggacaaca tcgggaatgc tgtgcattaa gaggaaatgg
    12541 ctggggaaag aaacatgcta ctgtggatta tgtttttcac tattctcctt atagattatt
    12601 ccaatatcat cagcttgtca tggatgaaat ggaaaaacgt ggttacagcc cagcaccaga
    12661 gtggaaaaat cctatgtatc gtggcaaatc atgcgaagca ttttttgatt tacctactgt
    12721 agagcgcaac gtgccgattt atccggaaca cgatgaagtt tatctagcag aatgtttagc
    12781 aaatttggag caaaaaggaa tttatcttac ctaatataac tagaaaagcc ttctatctcc
    12841 tcttacttca ttaaaaagct ttaaccatct tagatcaagc taaaatgtcg taagttttag
    12901 ggttatcttc atcctcacgt gcaaaactaa aagcgtactc tagttttggt tcatcgccaa
    12961 cttccctaga cataaatgaa catctcctga cgtggtttaa aagtaccgca tcttcgacgt
    13021 ctgtatacga tgcacagcac agattgcttc ttttgatctt tcttttgatt tttctgttgc
    13081 ttcttctaca ccttcttcga ttactacatc gatgcttaca attgcttctg cctaatattc
    13141 ccatgcatcg tcgatttaag cctctgtagt cactgtgatc gtttccacat tttaactcct
    13201 ctgtcgtggc ttcagttgat tttgtgatca gttcagttgc taagccagag tcagaacaac
    13261 ctgccattgc tagcgttaat attgatagta aaaatagttt ttttatcat
//
LOCUS       DAMYXM010000067        12497 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.070, whole genome shotgun sequence.
ACCESSION   DAMYXM010000067 DAMYXM010000000
VERSION     DAMYXM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.070"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..923
                     /locus_tag="PNS21_002216"
     CDS             <1..923
                     /locus_tag="PNS21_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HDI5159913.1"
                     /translation="DGHFSPTVFERYQRNEKALMASMLEMYVSGVSTRKVSTIVEELC
                     GKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPYLMTDVLYIKVREENRVLSKSCHI
                     AIGITKDGDREIIGFMIQSGESEETWTTFFEYLKERGLQGTELVISDAHKGLVSAIRK
                     SFTNVSWQRCQVHFLRNIFTTIPKKNSRSFREAVKGIFKFTDINLAREAKNRLIHDYI
                     DQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNQ
                     TSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            complement(1077..1607)
                     /locus_tag="PNS21_002217"
                     /pseudo
     CDS             complement(1077..1607)
                     /locus_tag="PNS21_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            1679..2971
                     /locus_tag="PNS21_002218"
     CDS             1679..2971
                     /locus_tag="PNS21_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDI5159914.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSECRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            complement(3183..4025)
                     /locus_tag="PNS21_002219"
     CDS             complement(3183..4025)
                     /locus_tag="PNS21_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDI5159915.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
     gene            complement(4062..5528)
                     /locus_tag="PNS21_002220"
     CDS             complement(4062..5528)
                     /locus_tag="PNS21_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDI5159916.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            complement(5584..7428)
                     /locus_tag="PNS21_002221"
     CDS             complement(5584..7428)
                     /locus_tag="PNS21_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDI5159917.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            8030..8980
                     /locus_tag="PNS21_002222"
     CDS             8030..8980
                     /locus_tag="PNS21_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDI5159918.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKKQIIENTLFSESKNFSKAIDVSPKVTSVA"
     gene            complement(9025..9658)
                     /locus_tag="PNS21_002223"
                     /pseudo
     CDS             complement(9025..9658)
                     /locus_tag="PNS21_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(9918..10466)
                     /locus_tag="PNS21_002224"
     CDS             complement(9918..10466)
                     /locus_tag="PNS21_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159919.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(10467..11324)
                     /locus_tag="PNS21_002225"
     CDS             complement(10467..11324)
                     /locus_tag="PNS21_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDI5159920.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(11610..11897)
                     /locus_tag="PNS21_002226"
     CDS             complement(11610..11897)
                     /locus_tag="PNS21_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDI5159921.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(11887..12216)
                     /locus_tag="PNS21_002227"
     CDS             complement(11887..12216)
                     /locus_tag="PNS21_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDI5159922.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(12293..12439)
                     /locus_tag="PNS21_002228"
                     /pseudo
     CDS             complement(12293..12439)
                     /locus_tag="PNS21_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
BASE COUNT         3849 a         2501 c         1990 g         4157 t
ORIGIN      
        1 gtgatggcca tttttcaccc acagtgtttg aacgttatca acgaaacgaa aaagccctca
       61 tggcttcaat gttggaaatg tatgtatcag gcgtttcaac tcgtaaagta tcaacaattg
      121 tggaagaact ttgtggtaaa tccgtctcta agtccttcgt ttctagctta acagaacagc
      181 tagaacctat ggttaacgag tggcagaatc gtttattatc agaaaaaaat tatccttact
      241 taatgaccga tgtactctat ataaaagtac gagaagaaaa tcgagtactc tcaaaaagct
      301 gtcatatagc gattggaata accaaagatg gcgaccgtga aattatcggc ttcatgattc
      361 aaagtggcga aagcgaagag acctggacaa cattttttga atacctaaaa gaacgcggtt
      421 tacaaggtac ggaactcgtt atttctgatg cgcacaaagg attagtctct gccattagaa
      481 aatccttcac caacgtaagt tggcaaagat gccaagttca cttcctaaga aatatcttta
      541 ccaccattcc taaaaaaaat tcaagatctt tcagagaagc tgttaaagga atttttaagt
      601 tcacagatat taacttagcg cgtgaggcta aaaatcgatt gattcatgat tatatcgatc
      661 aaccaaaata ttcaaaagct tgcgcatcat tggatgatgg attcgaagac gcctttcaat
      721 ataccgtaca aggaaattcc cacaatcgac taaagagtac caatctaatt gaacgactga
      781 atcaagaagt acgcagaaga gaaaagatta ttcgcatctt ccccaatcaa acatcagcca
      841 atcgcttaat tggagccgtt cttatggacc tgcatgatga atggatttat tcttcaagaa
      901 aatacatcaa ttttgataag tagaaatggt aaaaacattg tatagcattt tacacaggag
      961 tctggacttg actaacattt ccatttcatt tacctctttt tcgtttaatt atactaaagt
     1021 tctatccaag cgacaagctg tgtataataa aaatataccc gtggtgctag ttaattttaa
     1081 taccctaaag aatatctcaa agtcccttca aaatgttgct gtgctcggct taaaagaata
     1141 ctgtacgata ataatatgct ttcaaactta ctttctagcc cttgaataga acgtgatttg
     1201 aaattttgaa aacctagtct ttcgagagaa gaaaaaacag tttctactgc ttttctttgt
     1261 ctagtaagta atcgatggtc tatcttccct agtttatcca tgttttttcg tgacggaact
     1321 aagactgtaa tgcccttaag ggcaagcttt tcatggattt tatggctgac atagccttta
     1381 tctccaagga tatttggaag gtttgcttct tccgataaag tctctaaaac tttcacatca
     1441 tgaacactag gatccgtgat tgaataagta actggaaagc ctgttttagt gacgataata
     1501 tgaattttaa gaccataaaa ataagcgttt ttcgttgagt tatagcccct acagctctat
     1561 actttttgat gttggtggat gacaccagca tcttttttat tttccaaaac atatagaaaa
     1621 gacaccttga aatcctttag aattaatttg gcgaaccaac ccaaaaggag gaatcaagat
     1681 gtcttacact caccttatca aagaaacatt agatattcta gacttatcag ttacttttaa
     1741 cgaaaattgt ctcacaaaag aaaaatacaa agggcaaatc tgtcatattt atcgtggaaa
     1801 cttgatttat acagcccaag aatgcatcca ctgtaaacac cagattgctt cagatattgt
     1861 gcgttggggt acaacaaccg tccggttgtt aatgaacgat gtttctgaat gccgtaccta
     1921 cctcgaattg aaaaagcaac gcttcaaatg caaagcttgt cagcggacct ttgtcgcaga
     1981 tacttctgtt gcgaaaaaac actgtttcat cagtgagaaa gtccgctggt ctgtcgtgac
     2041 tcgtctgaaa aaaaatacct caatgacaga gatcgccgct cagaaaaatc tatcggtctc
     2101 ttctgtttac cgtattatga agcgatttta tcgtccgttg aatccttttc ggacaccgtt
     2161 acctaaggta ctttgttttg atgaattcaa atcagttcgg ggtgtttcag gtgctatgag
     2221 tttcatcatg atggatggac aaacacagcg cttgttggac attgtcgaga accgacaact
     2281 gcctttttta aaacgttatt ttagtcattt ttcacgagaa attcgtgaag cggtcgaatg
     2341 gatcgtgatt gatatgtacg ctccttatgt atcgctcgtt aaaaagctgt ttccaaaagc
     2401 acagctgatt attgaccggt ttcacatcgt ccaacatatt ggtcgtacct ttcgcaatca
     2461 tcgaatcaaa gaaacgaacc aactattaaa aagcaaagaa caaaaacact atcaattagg
     2521 taagcaatta aaaagatact ggaaacttct tcaaaaagat gaacgcaagc ttgattatac
     2581 tcgacgcctt tggcgacctg gcttcaaagc tcatttaaca gagaccgata ttgtggatcg
     2641 cttactgaaa gggagccctg ctttgcgtgt aggctatcag ttatatcaag actttcttta
     2701 cgctgtaaag gaacgagact acgtatcgtt tgaagaacta cttaccaata atatcatgct
     2761 tcccgaaggc tatcagacga ccttgagaac gttccagaaa tttctgccac aaatcaaaaa
     2821 tgctttacaa caaagttatt caaatggccc acttgaatgt ctgaacaacc atatcaaagt
     2881 gttaaaacga aatgcatatg gctttcgtag cttttataat ttcaaattgc gcatcatgat
     2941 tcgtcatggc aacgcattga ttttcaacta aaaaagatca agaggaactg atccacttga
     3001 tctccaatta ttctaagatt tctctttacc aacatcattt gacaaagagc cgagattttt
     3061 tattatctta cgttaatcct ttttaaatgt ctcttataga taacaaacag ctggaaatca
     3121 ccttgttttt actataatcc atctctaaag attttaaaag catcaatagt attactgagc
     3181 gctcacccat tagcatgttt catcaaccga ttgatgtgga cacctaggta tacaacttca
     3241 tcgttcggaa ttgttttatg ataaactttt tcaatgtagt ctttgatctt agctgcgctt
     3301 tccatggcag acgggtataa gatccacatt tgattgtaga attcttcctc accatcatcc
     3361 aacaaagcat cagaataata tctttctaca aagaaacgta catgtgtaat gaagcgggca
     3421 taatggatcg aattagtatc gatatctttc tgcaacgaat accggaccaa attgacgatc
     3481 ccgccgatca tttttgcata cttgaatcca gcgccgctat caggatcttc cgattgtgca
     3541 ttgatcaaat ggaaagcaat gtttgaggct tcctctttag gaagatcaat tccatattgc
     3601 tcgttcaaaa gggtgagtgc gtacttccca atttgaaatt ctttgggata gtagattttt
     3661 atttcccagt acaaacggtt tgcaatattt accccttttt gtgcgcgttc aactgcaaaa
     3721 tgcaggtggt ctgttaaggt taaatagact ccaggattca gttttttatc taatcctttt
     3781 tctgcttcaa aaacgatagt acgagtgatt tcaaaaaatt caataggtat ttcttcaact
     3841 aactctaata gttgtgaaga tttcttttct tcaaaaggca gaaatatttt atctacttta
     3901 tctcctgata tttcatcccc agctttctgt ccaaagccta tccctttacc taatgcaatc
     3961 atttctttgc cattttcttc aacaagtacc acacttgaat tcaatacttt ttttattgaa
     4021 atcactcatt atccccctat cgctgcttaa aaagatcgag actactcgaa taaggatgca
     4081 ccgtttgttt cgatcacttc tttgtaccag ttgaacgaat cctttttcaa gcggttatat
     4141 gtcccatttc cttcatcgtc tgcatccaca taaataaatc catatcgttt agacatttga
     4201 gaagttcctg cactaactaa atcaattggt ccccagctag tataagctat caaatctaca
     4261 ccttcatcga ttgcttcttt catttgttgg aagtgtgctt taaagtagtc gatgcggtac
     4321 ggatcatgga ttttaccatc tactacctga tcgactgctc ccataccatt ttccacaatc
     4381 atcaacggta cttggtaacg atcataaagt tcgatcaacg aaattttcaa accttttgga
     4441 tcgatttgcc agccccaatc tgttgaaggc aaatgaggat tcttaacccc taaaacagta
     4501 tttcccggtg tgcgttctgc gtttggatca gctgattctg ccatggacat gtaatagctg
     4561 aaagaaacaa aatctacggt atgtttcttt aaaatttctc gatcttcatc ttccatttta
     4621 atctgaattc cccgattttc caaatccttt tcgattagag caggatattc tcccttaact
     4681 tgaacatctg cataaaaatt attttctaag ttctttttca aggtcagctc aacgtcttct
     4741 ggtgcgcaag ttaatggata agtcatcaat ttcgttaaca tacaaccaac ttggcttcct
     4801 ggaatttttt catgagcatc ttttgtcact aacgcactcg caacaaattg atgatgcaat
     4861 gcttgataga tatcttgagc ttcttgacct tctgcgcttt tctcttggat aattccagct
     4921 gttgtaaatg gatgacggtg gatgctgtct atctcattaa atgtcagcca atattttacg
     4981 tatttgccga agcgatcaaa acaaacgtta gcgaagcgaa caaaatcatc aattactcga
     5041 cgctccaccc agccattata tttaactgct aaagctaatg gcatttcata atgagatagt
     5101 gtcacaatcg gctcaattcc taaacgttgc atttctttaa agacgttttc ataaaatgca
     5161 accccttttt cattcggttc cagctcttct cctgtaggga acaagcgagt ccaagcaata
     5221 gagaggcgca aggttttaaa gcccatttcg gcaaataaag ctaaatcttc tttatagtga
     5281 tgataaaaat caacgcccct tctttttggg taccatttat cagatttgtc ttcactagct
     5341 tcttttatag actgcagggt gacatgcata tgagcagcaa aatctttgtt gtctaaattt
     5401 ggtttataag tagccatatc cgctactgaa agacctttac cgtctacatt ccaagcacct
     5461 tctacttgat ttgctgctat cgcgccgccc cataaaaatc cttttggaaa acttgttgtg
     5521 tttgtcatta ttctattctc ctctattcct tcattcaata tatttttttt cactatcttg
     5581 tgtttaagct atcggactga tagctaagag atcactatca ttggttacca atttttctag
     5641 gttaaatgaa cggatagtgt aattatcctt attggttacc acacagataa ctgtggggtc
     5701 gaatcctgct tctttgatta aatcaatatc aaatgcaatc agcttttgtc ctttggctac
     5761 tttttcacca gaacgaacaa atgcctcaaa cccttttcct tctaactgaa cagtatcaat
     5821 accaatatga aaaagaagtt ctgcaccatt agccgttttt aaaccaatag catgcttcgt
     5881 ttcaaatacc attgtgattt ctccatcttc aggagctact aatatccctt cttcaggaat
     5941 gattgctact ccatcaccta ccataccact agaaaaaaca gtgtctttta cttctgatag
     6001 tgagattact tttccattca cgggacttga aagaacagca tctgaaacaa cggccgtttc
     6061 catcttagtt tcaacttcat tgacaacttc tttatcttta taaagaacca acgctgcagc
     6121 aaaagcaacc acaaatgaaa tccccgatac tgcaaatgcc caaaccagat tcattgggtc
     6181 agctttgtca gcgaacatcg tgatacttgc aatcccagga cccactaaag caaaggcttg
     6241 aagtgcaacg attcctgcaa atgctccccc gatcaggcta gaaaacataa cggcatataa
     6301 aacacgtttg tgcagaatcg ttaccccgta aagagctggt tcagtaattc cgaataatgc
     6361 agatataccc gcagaaacag ccgtagattt caattttttg tcttttgttt tgatactgac
     6421 agcaaagcat gcgccagctt ctgaaatatt gtgtgccaat gaagccggga gataaagaag
     6481 ttctgcaccc aattcactca tggatgaaac agcgtaaggg atcattgctt tatgcatacc
     6541 cgttacaacc attaatggca gtacagctgc taagactccg gttgcaaccc atccaaattg
     6601 attgtacaaa gacacaatga tagaagagaa agctgttcct acattataac ctaatggtcc
     6661 tagaagcaac aaagcaatag gaactgtaat caataagctc atcataggta caaagaaaat
     6721 acgaattggt tttggtgaaa cttttgtaaa ccatttttca agttgagcat aaaataaaac
     6781 agttaagatg gctgggaata cttgagatgc atacgctata ttatccaatc caaaagataa
     6841 gaatgtagca ccttccgcca acatagctgt catttcagga aggatcaatg caccgacagc
     6901 agatactgca accaattgat tgactttcaa tttattagcc gtagtcattg ccaccagaat
     6961 aggcaagaaa taaagcggtg ccccgccgac caaatttaaa atctgatagg tttgagattg
     7021 gtcactaatc agccctaaga tagaagctag tagcaacaag gacttcaata ctcctccacc
     7081 tgcaatcgca ggaattaaag gttggaaaat agaaataata aaatctaata gaactgaacc
     7141 taatttttgt ttttgttttg gtgcttcttc agtatttgaa acattgtcac ctaataacgc
     7201 tttcacttca tcatacactt caacaacatc gtttccaatg attacttggc attgcacatt
     7261 ttggcgaacg cccataacgc catccacttt ttctaactgg ctcatattta ccttgctgtt
     7321 gtcttttaac gtaaaacgta aacgagttga gcaatgctcc aaatgggata tgttttcttc
     7381 cccaccgatg tactgcaata cttcttctgc tgtttgtttg taattcatgg tgtttccctc
     7441 ttttcttttt tgagtgatga acttttgagt cgaagtggcc atcctcaaca caaaaaacag
     7501 gcagaaacta aataaattga caacaaggca aacgtgtatt gaaacgcata cattgttatt
     7561 caacttattt aggtcctgcc tgcattacca gtcacatgcc tgtttaagtt atattcaatt
     7621 taacatatta gaatgtaaac gtaaataata aaatgatttt ttaatcattt tttcatttta
     7681 ttcgttcaat atagttttcg tgttctaaaa gttttgtctt ttttctaaga atatcttcca
     7741 acatatataa ctcttattct cttctatctt ctagcagatg ttctgtcttt ttaagaccta
     7801 gtataaaaag aatgggttca ttttttcttt cttgcaggat gaggtttttt atataaactt
     7861 tatttgatat aaagaaaagg cattcataat tgtgctatac tactaaaaaa tgataataca
     7921 gtgaattaga ttagcgcttt attaaattaa gaggacagca tatcgatatt gcttcctttt
     7981 ttttgctatt tatttaaggg atagaagttt agaaaggact gtttataaga tgaaaaatga
     8041 tatagtgact tatgcaaaca caaaatgtga agaaattggc atcagtaatt tagaagagtg
     8101 cttttttgac acaaatgacc tgtcgcaatt tttaagaacg ggaagaacaa atgccagtaa
     8161 acttgccaat gacttagttg caaagaacgt tctatttaaa atcaatactc gtccagtgtt
     8221 atttgccatc aaagagcgct ttgaagagca ctttttgatt ccggtaaaag atgtatacga
     8281 aagtatatct gaattaaaaa cagacttgga gcccaattct acagattcca ttctacaaaa
     8341 gatgattggg tatgatatga gcttatccga tgcggtggaa caaatgaaaa ctgctgtctt
     8401 ctatcctgga agagggcttc ctatgatgat cactggagat actggagttg gaaaaagttt
     8461 ttttgttcat actgcttatc agtttatgaa aaaagtgaac gttattcatg agaatgctcc
     8521 ttttaaagta cttaattgtg cgcaatacca taataatcct gaattattgt cggggatact
     8581 atttggctat gttaaaggtg cttttaccgg cgcagatgaa gatcgaaatg gattgttgca
     8641 agatgcagac ggtggagtac tgtttttaga cgaagtacac agattgcctc ctgaagggca
     8701 agagaaacta tttgtttata tggatggcgg aacttattct agagtcggtg aaactatcca
     8761 tcacgcctcc aatgtacgtt tagtttttgc gactacagaa ggcaaagagg attttctgga
     8821 aacctttatg cgaagaatcc ctatacatat ccatatccca aatattgagg aacgaggtat
     8881 tttagaaaaa aaacaaatta tcgaaaatac tctctttagc gaaagcaaaa actttagcaa
     8941 ggctattgat gtttctccta aagtcactag tgttgcataa atatagtcag aatattctga
     9001 attattattt tcttgaaaaa agtaaaaaaa atcgtatccc aaactttcat ctacatttcc
     9061 agaaagatct tcatgttgat aatcaattcc tgaatcaatg accgctacct tcacatcctt
     9121 agaacccttg gtttcttccc aagcggatgg catgctaatc tctcttaaat tagcttgttt
     9181 actccaatta ggatcgttat cgatttccta aagaatcagt tgtatttggc tcctcaacat
     9241 cattcaacgg ctctagttca accacatagt ttggctctgc ataattgacc atatcagaag
     9301 cattaaatgt tcttactgcc tcttccacac taatttcttc gttcaatttc actaataata
     9361 cttgatctcg cttattataa tcgctttcat ttgtcctaag agtcaaatct ttttccacca
     9421 taacttttac acccaataaa ttttgcggtt tatttttctc tgttgaaatt ctttgtgtag
     9481 cctttaaact gacaattagc tcattgggta catactctat ttgagagtca tctaattggc
     9541 tcataatcct ttctttttgg ttagtatctg cagttacttg tttagtatga gccatgaacc
     9601 caaacatact caccccaaca accaatccta aatatatttt ctttttaaac tttttcatct
     9661 acatctcctc ctaatagttt tattttagag ttagctctat tacttattta ctaactcaac
     9721 aacagattat catacactta ttaatgaaat cacaatatct cattaaaaca caccctaaaa
     9781 tcataaccgt tcctatgctc attttttgga ttttttaatc acgtaaccag aaaaataaat
     9841 cccctctatt tcacgcaaat caaagaagaa ctaagtaact accgtttaaa ggtagttact
     9901 tagttcttct ttgattatta tctaacttta ttattcattc cccacccttt gcgcagtgag
     9961 tcttcggtat cttgttttat ctgactggta gttcttatta tttctccacg atcatctaat
    10021 tctggaacaa ttggatattt ttctctgaac aagtctagtt ctttcaccac gtcattaaac
    10081 aaatcttgtt tatcttcttg cgtaagattc tgtttaatat actcatctaa agcttttcta
    10141 agcaattcat ctcttgtcat attagtctca tacttttcta ctaaaactct ggaagtcgca
    10201 tttaacaaag tcgacagttt taaaggatgc ttggtggaat aaaccttatc aaactttctg
    10261 attggtgcgc caactttttt tgttcttcct ctactctgtc tttcaacatt ggaggaagtt
    10321 tttatttctt gaacttggtg cacttctaat tgcttatttt cagatggtgt aattgcttgc
    10381 tgctttttct ttgcttctgc ttcttttttt attttggcga gtcctttgtt gttcccaaaa
    10441 atatcaagtc ctttgatgtc agccaattac tccacctccg cataccaatt ctcgtgttct
    10501 tccaattctt tcaaaatact gataaataat tcgtggcttt tgcgatccca ttgttttaag
    10561 agacctttct ttgtcttctc catcgtaata cctgatccgt cgtatcgttt taagcgcgca
    10621 tttttgagta caacattttt aatcaaatgt tccccataaa tctcttgggc ttgtttatac
    10681 atttcctgat ctactgcgtc accgctttcc aacatgaaag gaataacacc agcaacatat
    10741 aaatttgtat caaattcttt cacaaaaaag tccatatact caagatattg catcgcattt
    10801 ctcatcgatt ttacttgggt ctgtaagacg acgattgccc aatccgcaat gtacatcgcg
    10861 gatttactat atacagaaat agaaggtggg acatctaaat agatgacgtc aaactcatca
    10921 aataatggtt ctagcttttc tttcatatat ttgaattggg ataatgaatc attatattgg
    10981 tctttgatcc aatcatcata catttcaaag gacatgtctg ctggaagtat atagaggtta
    11041 ttgattactt tatagacagc ctctttaggt ggaagattgt ttatcaacac gtcagtcaca
    11101 gtattttcgt aaacaatcgg ttcaccttct ccgcctcctt cttcgttagt gaagttcata
    11161 tgcgttaagt tcatcacatc agaagtattt ccttgcatat ccaagtcaat tatcaaggtt
    11221 ctttttcctt cattaactgc tgattcaaac cctaacattt gcgcgcacgt acttttacct
    11281 acgccacctt taaaattacc gattacatat ttcttcgcta ccacgttcct acctcttttc
    11341 ttttagcaat attcatttga tactattata acatatttat ttttagttac taccaaaaaa
    11401 aagatatata tgtactacct tataattaat aagtaaaagg tagtacataa ataaggaaaa
    11461 gctgaaatat attgatttat agctttcctc aaagtaatga cgttatttaa tttatcgaga
    11521 agcagagaat actcgcgact ttagtcatgt agacgaatca gattttcgat aaaaaagaag
    11581 ccgtccttct taagaacgac aacaaatcac tagattaatt ttttcaactg ttcataaaag
    11641 ttttcatgcg ttccacataa caatactgga ataaccttgt tttctataat ctcgtaagct
    11701 actctatatg ttgtgcccgc atatttgaac ccactagacc aaatacctga taaatcccca
    11761 gttttttgtt cacccttata tggatcaatc gcaatttcat cataaattaa attcaaaaat
    11821 ttttctttca atggcttctc ttttagcttt tttaaatact taatgacatg ttttgaagga
    11881 acgatttcat aagccaattt ctgttgccaa ctcctcttta gtcattactt tagaattagt
    11941 aagtgtatct tcagcaatat ctctaaagga agaatgcatt ttgttttttc tattcttaaa
    12001 ttctacaaga atctcatctt tatcgtagcc ttctgcaatt atatcagaca aaatatcttc
    12061 gctaaaatca taaaattctt tttgtggttc aacaaattca taaagaatac cattctctac
    12121 caactttgct tcaacttcga ctccattagg aacattaaaa tctttaggta ctgtcaacat
    12181 gattgaattg ccttgttttc ttgtttttgt aatcatttaa atccctcatt tctaacagta
    12241 tgtaattact tagtaaatac attataccac gagataatgt atttacaagt aattactgtg
    12301 actcgtaatg cgtccttgct gaataaattt caaccacgtg ttcatcttca tctaccttat
    12361 aaacaacccg gtgttgtcta ttcaatcttc taaaatacct accttcatgt gtaggtttta
    12421 acttctcaaa tgattgggtt ctgttgcaaa gttttaaata aagaataaaa tcccttacgg
    12481 tatctatgat ttaagct
//
LOCUS       DAMYXM010000068        12466 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.071, whole genome shotgun sequence.
ACCESSION   DAMYXM010000068 DAMYXM010000000
VERSION     DAMYXM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.071"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..340)
                     /locus_tag="PNS21_002229"
     CDS             complement(<1..340)
                     /locus_tag="PNS21_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI5159923.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVSQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVR"
     gene            992..1963
                     /locus_tag="PNS21_002230"
     CDS             992..1963
                     /locus_tag="PNS21_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDI5159924.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     GSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(2041..2277)
                     /locus_tag="PNS21_002231"
     CDS             complement(2041..2277)
                     /locus_tag="PNS21_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDI5159925.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(2246..2377)
                     /locus_tag="PNS21_002232"
     CDS             complement(2246..2377)
                     /locus_tag="PNS21_002232"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDI5159926.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(2453..2836)
                     /locus_tag="PNS21_002233"
     CDS             complement(2453..2836)
                     /locus_tag="PNS21_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDI5159927.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(2869..3654)
                     /locus_tag="PNS21_002234"
     CDS             complement(2869..3654)
                     /locus_tag="PNS21_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HDI5159928.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            3832..4932
                     /locus_tag="PNS21_002235"
     CDS             3832..4932
                     /locus_tag="PNS21_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDI5159929.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4922..5746
                     /locus_tag="PNS21_002236"
     CDS             4922..5746
                     /locus_tag="PNS21_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HDI5159930.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            5743..6558
                     /locus_tag="PNS21_002237"
     CDS             5743..6558
                     /locus_tag="PNS21_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDI5159931.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            6555..7385
                     /locus_tag="PNS21_002238"
     CDS             6555..7385
                     /locus_tag="PNS21_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5159932.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            7392..8423
                     /locus_tag="PNS21_002239"
     CDS             7392..8423
                     /locus_tag="PNS21_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5159933.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(8530..9803)
                     /locus_tag="PNS21_002240"
                     /pseudo
     CDS             complement(8530..9803)
                     /locus_tag="PNS21_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            10080..10289
                     /locus_tag="PNS21_002241"
     CDS             10080..10289
                     /locus_tag="PNS21_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159934.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            10339..11055
                     /locus_tag="PNS21_002242"
     CDS             10339..11055
                     /locus_tag="PNS21_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDI5159935.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(10957..11477)
                     /locus_tag="PNS21_002243"
                     /pseudo
     CDS             complement(10957..11477)
                     /locus_tag="PNS21_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            11765..12418
                     /locus_tag="PNS21_002244"
     CDS             11765..12418
                     /locus_tag="PNS21_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI5159936.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT         3626 a         2292 c         2819 g         3729 t
ORIGIN      
        1 ggcgaacata attctttcca aaagttgaga aatgggggat tttctcctcg aaggagtagc
       61 ctaaaaacca gcggtaagcg acattggtat cgacttcctt aatggtttgt cgcatggaac
      121 ggataccgaa taaatactga ataaacacca atttgatcag aatcactgga tcgatacttg
      181 ggcgacctaa atcggagtaa gtggcttcga cgattggata aataaattca aaatcaagcg
      241 cggcatcaat ctttcgaacg agatgatctt gagaaactag atcatcaatc gtaagcatcg
      301 caaattgatt tctaccattc gtattgtttt tagacatcat agctatcact ccttgcattc
      361 gttttcataa atctattata aaagaaaaga ctgtagacat catccataaa atggactttg
      421 tctacagtct ggataacgtg attttttgct gtaatgacaa ggttttagct gtttcagata
      481 ccatgtatcc aaaaatagtt tattgagaaa ataggagagt atttgatact aactagtagg
      541 aaaaaatgag ataaaaagta aatggaatca tttaaggtaa tctgaaataa aaaatataca
      601 gccctgtggt actttaccta caaggctgta tatatttttt actctcagcg tttttgactc
      661 cgtgtctgac tccgttttat tgattttgaa tgttattcaa tgaccattta acaggtcaaa
      721 tttgatgttt ttgattccgt atgacgccgt ttgatatcta gtgatatagt gatttgagtc
      781 tacatcgaat aagggaataa accaataaaa aatgatttag aatattaaaa taacaaaaaa
      841 gaggactacc tgtattagtc ctcttttttg accagatttt gaccagtcta ttttacatcc
      901 tagtatattt atatgagcta attttatgat taaatggtaa gtttttcctt gtgtgtttta
      961 gtttaaacta gtgtgtaatt caaaaatcta tatggaagga acagtattaa gaatagaaca
     1021 gggttctcta catgatggtg ctggtttacg gaccgttgtt tatttaaagg gctgcccatt
     1081 acgctgtgcc tggtgttcga ttcccgaatc acaaagcaag cagattgaaa aaggatttgg
     1141 ccagaccatg accgctgaag aggtaatgga tgagattgag aaggatgctg ttttttactt
     1201 tcactcggat ggcggagtga ccattagcgg aggcgaggca ctggttcagg cggattttgc
     1261 taaagaaatt ttgcaaaaat cgaaatacat tggaatcaat accgtactgg aaaccagctt
     1321 ttgtggtgct tataatgaaa tccaaaaggt agctccctat gtggatacat tgtttgtgga
     1381 tgtaaaaatg tttaccagca agctccacaa acaatggacc ggcttagata accaacaaat
     1441 tttgaagaat attcgtcgct ttttaattga gtatcctaac tgcgaagtgc gaattcgagt
     1501 tcctgtggtt ccaggtatca atatgaacct gaccgagctg ctgacgattg cttgttttgt
     1561 ggcagatctt gatcggttcg taccgttaga attactaccg taccactgct atggtatgca
     1621 cggttaccaa gcattgggac tggagtatcc gttggcagac acgccagcgc cttcacctaa
     1681 agaaatgttt gcattggcag atcaactggc tcgcacggtt cctcatttgc tggtcacagc
     1741 tttaaccaaa acatttattt atttattgaa tccaagcttt atcttatgga taaatgaaag
     1801 acactcacta gctgcagccg gctcaagaat agagcttttg ctgaagtcag tgagtgtctt
     1861 tttacgatgt tttttcgaag ttaatttgtc gaatgaacac tggctcaatc ggtacatctt
     1921 cactgtttgt cggctgagct gcgatcgctg ctacgatttc taacccttgg tatacctgtc
     1981 caaaaacagt ataacgacca tccagctccg gtagtcctcc aagctccaag tatgcttgct
     2041 ttacattagc aggtcagcct gcgcctttgt gttttgcaca ataaaaaact ggttgccgtt
     2101 ggtgtggggg ccgtggtccc ctaaggccaa ggcgccatta aaatgataaa gtccgcgatg
     2161 cacttcatca gcgaaaaagc cctcatagct ggaaacagct tcatcaccag aaccatccaa
     2221 agcgccacct tgaataacaa attccttaat gacacgagcc gaaaggtgtg ttgtcataaa
     2281 cgccttgctc agctagggag cgccagttgg ctacagccag gggagcttgg tcaggaaata
     2341 agcggagacg aatttcacca gcatcggtga ccagcacaca aacttcttcc tgctgcggtt
     2401 tttctaattg aaagagagga aggtttgcaa ctgaaacgcc ttgatcctgc catcaaatat
     2461 cgaaaatccg ccactcgacc ttcgcctgat caccaaggcg agccattccc aataaaacgg
     2521 tataggcggt ttcatccgct tgaaaggtca attgcagctg tcctcgtaaa tcgttgtagt
     2581 catctggggc ggctacttga taaaaaagat gaaactgcgc atgcaaatat tccaaaggct
     2641 gatctttaaa ggctgcaaaa taagcctgaa ctttttcccg ctgcaagctg tcagccacca
     2701 aaaaattttc cagttgttgt tttggaacgg ctgctaacaa ggatcggcaa accgcctcaa
     2761 ttttcaacgc atcccaatgt cgcgcaatta aataatggat gggtgcctct tgattgtagt
     2821 cccacaggtt gctcattttt ttcaccactt tcttctatta tataggaatc acaaaatcgg
     2881 cagcggcgtc aggcggcgtt cttttccttg aatgactagc aacttttcta cctgacagtc
     2941 tgccaacagc tgactcattt ttttaaagcc cagctgataa tcttcggcaa catgagcatc
     3001 cgagcctaag gtaaagagct ttccgccgac actttgatac aaaggaacgg cataacggta
     3061 aaggtcttcg ttgccgtatt tcaagaagct cttggcattt aactccatcg ccaattctcg
     3121 ttcaacaacc tttttgaaaa tcgttagcag ctgagcctca aaatgctccg cgaattcttc
     3181 cgctgtgaag gtgaagcggc gtaatccata atcaaagtga gtcaaaatat ctccttctgg
     3241 aaaatccgtc aagacctgca acatctgatc aaaatattcc ttggtagttt gaatgggatc
     3301 tttggttaaa accacatcgt ccatataatc aaagcggcca ttttgatgaa tacttaccag
     3361 ctttaaatca taaggatgct gagcgaggta atccacgatc cgctcttctt gtcctggaac
     3421 aacgccaatc tcgatacctc gcagaaattt ggtggaatag gtttctttta aagagtccag
     3481 tttgtttata taggctggat aatccggaat gtcatctttt ccggtagatg gattgtttaa
     3541 atcaaagtga tccgtagaaa cgaaaaactc cggctgatag gctaaatagt tttcaaattt
     3601 ttcctctgaa tcaaaggaaa aatacgtgtg gagatgctga tcataatact gcatagggag
     3661 cctccttttt tctactatca taccgcaaaa gctttagaag gttaacggga attcgtcgaa
     3721 atttacaaac catatacaac ctttacattg ttcatgaaac cttttcaaga aactgattct
     3781 ctcattacaa agctttgtta gaatggaatt atcgaaaggg gagaagaatg aatgaaaatg
     3841 aatgaaaaaa ttcgtaacat gactatcggt ttattttgtg cagcaacagt aagtatcctg
     3901 ctgacaggct gcggctcagg tgaagcaacg aagcaatcgg ctgcggcaga cgactcttta
     3961 gatacgatta tagaaaaagc caaggaagaa ggagagattg ccagcttggg aatgccggat
     4021 acttgggcaa attgggtagg cacttggaac gaccttgaaa aagaatacgg cttaagtcac
     4081 gtggatacgg atatgtccag tgcagaagaa ttggctaagt ttgaatcaga aggaaaaaat
     4141 ggaaccgccg atattggtga tgtcggtatc agcttcggtc cgttggctga aagcaaagag
     4201 ttaaccttac cttataagac caactattgg gaggaaattc cggattgggc aaaggatgat
     4261 gatggggatt ggttgttaag ctatacggga accatttcct ttatcaccga taaagagaat
     4321 gtgaagcaag caccgactag ctggaaggag ctgcaagagg gtgactatca agttagcgtg
     4381 ggggatgtga cgattgccaa ccaagcacaa tttgctgtat tggctgcggc gattgccaac
     4441 ggaggtgacg aaaaggatat tcagccaggt ctgaattact acaaaaaaat ggcagaaaaa
     4501 ggccgtttgt ccaccgttga tgccagcgta gcaaatcttg aaaaaggtga aatcgatgtg
     4561 gcagttgttt gggacttcaa tggactaaat tatcgtgacc aaattgactc cgagcgtttt
     4621 gaggtaactg tgccaacaga cggctccatc attagcgggt atacaacctt aatcaataaa
     4681 aatgcccctc atcccaatgc ggcaaagcta gcacgggaat atattttatc tgatgaaggg
     4741 caaatcaatt tggcaaaggg ctatgcgcga cctattcgca aggtagagct gcctaaagaa
     4801 gtagctgaaa aattactgcc ggaggatgct tatgaagcgg cggtgccagt tgaagacaat
     4861 gaagcttgga acaagacctc tgtagagctt ccagagcttt ggcaggcgga ggtactgacc
     4921 tatgcaaagt agacttgaca aactgattgt agttatacta gacggctgtc gctacgacac
     4981 agccgtcgcc cagcttgggt tcatgaatca tttagttgag caccagcaag caagcttatt
     5041 gaaggtaaaa agtgagctgc ccagcaattc acgtccgtta tacgaagtac tgatgaccgg
     5101 cgttccagcc tatgaaaatg gcatctatag taattaccat gctcagcgat ccaaagagtg
     5161 gtcgctgttt gaattagtta aggaagctgg cggaaaaaca ggagccgccg cttaccactg
     5221 gtacagcgag ttgtacaatc aagcccccta taatcgtttg gttgatcgca tccagcatca
     5281 ggaggagcga ctgattcagc atggcatctt ttatagtgag gacacttacc ctgattcgca
     5341 tttatttgcg gatgcccatt atttagtgaa tcagtatcag ccagactttt tggtcgtaca
     5401 ttcgatgaat attgatgatg tcggtcacaa atttaccgcc gattctcatg aatatgcagc
     5461 agcggttaat caggcggaca gattattggc tatttacctt cctgaatggc tgcaaaaagg
     5521 ttatcaagtc gtggtgactg cggatcacgg gatggatgcg cacggactgc atggcggcag
     5581 cttggctgca catcgtgaag ttccttttta tttgatttca aataaacgcc aaaagctgaa
     5641 ggaacaaact gtaccacagc tgctggcggc tccattgttt tgctggttat taggaattgc
     5701 accgtcagag aaaatgcagg acattcaaga attgatgagg ggatgaaaga gatgaaaaaa
     5761 cgcttggttc tggttcctgc ctcctttatc ttctgtgcat ttttactgct gccgctgctt
     5821 tttatggttc tgtgcagctt gcacagtgat ttaactaacc agtggacatt ggccaattac
     5881 agtgatattt tcagcaacaa atattacaca caggcctttg ttaacagcgt actgattgct
     5941 gtcagctcca gtgtgattgg gttaatcggg acgtttattt tatgtttatg cttaatgaat
     6001 ctatcgacta aattgcagga gaaaatcacc tttgtttcca atttggcagc taattttgct
     6061 gggattccgc tggcattctc ttttattatt ttgttgggga atgtaggtgt gctgaaaatg
     6121 attttgccat ttctagcgga cttcaatttg tattcttggt ggggattgac catcacgtat
     6181 acctatttcc aaattccgtt aggggtcttg tttttgtatc ccagcatgaa ggaagtgaaa
     6241 aaggaatggc tggagtccgc acaattgctg ggcgcttcat cagcgtatgc ttggttcaag
     6301 gtggctttgc cttttttgcg accatcgatt gccagcacct ttgtcatctt atttgctaat
     6361 ggcatgggga cctatgaaac agcgtatgcc ctgaccagca gtaatgtaaa tcttttaacg
     6421 attcgaattg cggctttagt gtcgggagat gtgttcgcca agcctaattt aggcagtgcg
     6481 ttggcagtcg ctttaggtgt cctgctggta acggcaatgt tgattattca aaagaaaggc
     6541 aaggtggtgc agacgtgaaa aagttaataa aaccgatttt gttctgcttt gtcctgtatt
     6601 acttgctgcc gattattgga acgattttgt atgccagctc cactaaatgg tcaaaatcgc
     6661 ttttacctag tgattttacc ttgcaatggt tccaacagct gctgacggac agggaattta
     6721 ttgctgcggt gggtcgctca cttttattag caggtgtggt cttggtgacg attctgttgt
     6781 tgatgattcc caccattatt tggattcatt tgtatttccc tcgtttgaat cgttggctgg
     6841 aaaagctgct cctgctgccg tatgctttac caggagtgat tttggtaacg gctttgcttc
     6901 gaacgtatgc tgaaactggt atcccaatgt ttgtagtgtt ggtaggtgca ttgtttatta
     6961 cggcgttgcc gattgtctat cttagtctta acaaccagat gcgcttaatt aatctgaagg
     7021 aattagtgga tgcagcagaa accttgggcg ccccgatgtc tacgattatt atccaggtgc
     7081 tgtttccaaa catccgtatc ggtgtgaccc tagtttctct gatgattttt tcttcggttt
     7141 ttggggagta catgctgacg aacctgttga ttggcggtcg atttcagacg gtacgcatct
     7201 atatgctgcg gcggatgaac gaaaacggcc atttggctag cgctgtgatg gttttgtatt
     7261 tgctgtttat ggtgggaatt gctttaacta cctttatgct gacttatcga caaaagcaac
     7321 agctgtccct taagcagacg agggaagaga agcagtcgaa aaagaagccc ttaaaggagt
     7381 tgtgacggaa aatgttttta gaaatacatg atctcaatct gttttttgat gaaaaacaga
     7441 ttttagctat tgatgaaatt cagattgaaa agggtgagct ggttacttta ctaggtccca
     7501 gcgggtgcgg aaagtctacg ttgctgcgct gtatcacggg attggaacaa ccaaagagcg
     7561 gtgagattgt gctggacaat gagaatattg ccgataagcc tacgaaggat cgcaatatcg
     7621 gctttgtttt tcaacagtat gcattgtttc caactatgac tgtttttgaa aatgtagcct
     7681 ttggtttgaa ggtaaagaag ctgagtcctg aggtgattca ggaaaaggtt tttgagatgc
     7741 tatcattggt ggatatgacg gagcaggcag ataaaaatgt ccagtttctt tctggcggtc
     7801 aacgacaacg ggtggcactg gctcgctcat tggtcactga accgaaagtc ctgctgctgg
     7861 atgagccatt aagtgctttg gatgcccgaa ttagaaaaca gctgcagcgg gatttgcgcg
     7921 ctatccagca atcgctaggg atgacgatga tttttgtgac tcatgaccaa gaagaggcga
     7981 tgcgaatcag tgatcggatt tttgtgatgg aagctgggcg ggtggctcag gtttccacct
     8041 cgaaggaatt gtatcagaat cctcagagcc gatttgttgc ggaatttatc ggcaactata
     8101 atcgtttcga atggtatgag ctgaatcagt atacccacga gttttccgct aagggcaatc
     8161 gctgcattta ttatttaaga ccggagctga ttcaatttga accggttcct cagggggtta
     8221 agattccggt tctatggaag gaatcattta ttttaggcaa cattcaacgg tatgtttttc
     8281 aaacgctgga gggcaaagaa attttagtgg atcggttgaa cgatcgggaa gagaaaatta
     8341 gcgatgcctt atacattcag ctgaaagata ttctagcaat ccctgtggaa attgatactc
     8401 agcattttgc acaacaaagc tagtgtatag tcggcaaaca tttctggctc tttgtcaaat
     8461 aggactgata gagtgatata agaacatcta aggtagaatt gattttctgc tttagatgtt
     8521 tttttatgtt taataagtga tcagatgatt gattaagaaa attctggttc tcatcctaac
     8581 ccgtaagcta ttcgtttcaa tgctttgatg tgggtatgca gattttctat tttgccattt
     8641 gagtaaggca actctaaggc taaacgaatg ccggattcat agtttataaa cacttttttt
     8701 atatgatgca gttctcttgg aaccgacttg ggcattgttt ttacaagctg aaagaagatg
     8761 tccgcttctt tgtcataaaa agcttgaaga atttcttgat agtaagcata agctattcgt
     8821 agttcatcag aagtagaaag tagacgttct acgacttcac tttcggttaa ataggcccaa
     8881 ccaaaattac gccattttgt ccatttatca taaacaagcg tgtcctgtcg tttgatcaga
     8941 agtcgagata aagccttcac ttgtcgatag tgtttggctt gtattggatc gtttcggtcg
     9001 aagcttttca tgactcatag tcaaatatcc ttaaaactgc gaatgaggtg cttgacgatg
     9061 tgaaagcgat ccacaacgag tttggcatga ggaaagcact cttttatcag caaagcataa
     9121 gaggcgttca tatctgttac gagtattttt acgtttaaac gtgcacgtcg ttcaaattgc
     9181 ataaaataag aaatgagatc tttcttctta cgtgaaggaa gaatatccag taatttccca
     9241 gtatcgccat ccatacaaga aaaagaatag ggaccgactt gatttttcaa tgaacgaaat
     9301 tcatctacca atgaacagcg aggcaaccaa tttattttca cgtttgtcat ggggagcaga
     9361 gaagcgagtg ttcgcgaaac ggttgaccaa gaaacgccgc aatgtttaga aatcgtcgtc
     9421 attgcttgtt tttcagtcaa ttcctccaat attttataat gtacttgttt ggcgataaaa
     9481 cagttttttt caacaatgta ggtattcgcc gtaaaatagg tgtgacagcc acgacagagg
     9541 taccgttgct ttgccaaagc aagataagtc cgttgattcg cacacttatt gagaaggacc
     9601 agcgattttt tctttccgtt cttaacgatc agcccatgtt gattcacctg tttacactga
     9661 ggacagggct gaatcgtttt aatgagcgtg gcagaaacaa cgatactgtt gattgaatgt
     9721 atttttttct tctcgacatg agtgatttga atgtttccat ctttgattcc tagtaatagt
     9781 ttgataagat gaagatcagg catacagaac gctcctttga ttgtgttgtg gtgattcaat
     9841 tctaacggag cttctgtatg ttttgttttt ttatgctcaa aaggactgat agaatgattt
     9901 actatcagtc ctagaaatta tagcgccaca tttctttcac ctttgacttt tcagacggca
     9961 gtttttatgc tgatactgta gagagagcag gacacagctt ttgcttgttt tgggtaaggg
    10021 ggaaagaaga tgaccaaagc acttattttt gattttgatg ggacgatgat tgacagtgaa
    10081 tggatgtttt acgagagtat tcatcactat ttactgaagc ctatttactg gagcagttcg
    10141 gctataaaat tccttttgca gactatcaac aaggtgttgg gacggcagca gaactgtcct
    10201 tttatgaagg gataagtcaa gcggttggga gaccagtgga tgctgaacat tttgagcagc
    10261 ggatcagcag cactggaaaa tcggagtagt ctcaattctg aactgcggga gctagactat
    10321 ttttttgaag cacatccgat gattaaggaa aaaattgatt cctttgtgac gatcgatgat
    10381 cttcaggtcg gcaaaccaga tccagaaggt tatttgttgt gcttgaaacg gctgagggtt
    10441 gaacaggctg ttgtagttga ggatagtcca gtcggtgccc aagcagcacc gcatatgaca
    10501 gttgatgtac aagcgttgga tgcagatttt ttcgccttta gcgggcataa aatgtgcggg
    10561 ccgacaggaa tcggtgtatt atatggcaaa cgtcaattgt tagaacaaat ggagccagtc
    10621 gaattcggcg gtgaaatgat agattttgtc aatctttacg atagttcatg gaaagagctg
    10681 ccatggaaat ttgaagctgg tactccgaac attgcaggag caatcgatta cttgaatgag
    10741 attggtatgg agaatattca tcgttatgag caagaactgg ttgcctacgt tttacctaaa
    10801 ttacaagcga tcggcggtat caccatttac ggaccgcaag atcctgaaca tcatactggc
    10861 gtggttgctt ttaatttgga tggcatccat ccccgtgatg tggcgactgc tctggatatg
    10921 gaaggtgtcg ctgtacgttc aggacaccat tgtgcacaac ggttcatcga cgatttctgt
    10981 ttgactaatt ggtcgtttaa acagtcgatg ataatgccgt ttgagcggct ccaatgtcgt
    11041 ggaatctttg agtaagagtt tccaatatcg cttgagccga cggtacttct tctgattttc
    11101 agattacgtg attgattttc gtacagttct atgagaatcg cctgcttcag ttcctttgtt
    11161 agttgatgat gttcatcaac taagtccgtt ttcgcagaaa aggtctgccc acaatcatag
    11221 catagaaaac gggtacgttt taattctaaa atagtcaact tttctttgac tttcaacatc
    11281 tgagtacgtg tcgtatagga accgttgcgg ataatacgaa tcgatccaca gtgttcacaa
    11341 tgggaaggtg agtcatccaa tctcccttta ataaactgcg cttgaacgcc tttatattct
    11401 ttttcaatca gccattcttc atcaaaaaat aaatgtttat ctgttaatcc gagtaatttt
    11461 ctagtatgat tatccataga gcgactcctt tggtagggtg atgttttggt cgactaaatt
    11521 ttaccaaaag tgagtctctt tttgagttga aacgaagaaa aagtgttagt agaaaatcgc
    11581 cttcgatttt ccaacaacac aaaatattat agaagccttt tttctaaagg atttcaaggt
    11641 gtctgtttgc atttattaaa aaaaagaaaa agtattggca acagaaggcg tcaccaacac
    11701 tagatattat agagccataa tatgctgtat aatatctagt gtataggctt aagtggaggt
    11761 atatatgaaa atattaactg ttgaagatga taatatgata agagagggaa taagtgaata
    11821 tctttcagaa tttggatata ctgttattca agctaaagat ggaagagaag ccttgtcaaa
    11881 atttaatagc gatataaatt tggttatctt agacattcag atacctttta taaatggttt
    11941 agaagtgttg aaagaaatta gaaagaaaag caatttacca attctaatct tgactgcatt
    12001 tagtgatgaa gaatataaaa ttgatgcatt tactaattta gtagacggat atgtagaaaa
    12061 gccattttca ttgccggtct taaaagctag aatagattct ttaattaaga agaattatgg
    12121 acatttagag aagtttgaat ataaggatct atcggttaat tttaatagct atacagctaa
    12181 aataaatggt gaagaaatag atgtaaatgc aaaagaacta gaagtattaa aatgtttatt
    12241 ggataataat ggacaagtgt taacaagaat gcaaattatt gattatgtat ggaaagatag
    12301 cgaagaaact ccttatgatc gagtaataga tgtatatata aaagaactta gaaaaaaatt
    12361 acaattagac tgtataacta ctataagaaa tgtaggatat aaattggaga gaaaatgaaa
    12421 aaattaaaga tatttccaaa aatgttcata caaatatttt ctgttc
//
LOCUS       DAMYXM010000069        12309 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.072, whole genome shotgun sequence.
ACCESSION   DAMYXM010000069 DAMYXM010000000
VERSION     DAMYXM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.072"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="PNS21_002245"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="PNS21_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDI5159937.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="PNS21_002246"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="PNS21_002246"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDI5159938.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="PNS21_002247"
     CDS             complement(2169..2906)
                     /locus_tag="PNS21_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDI5159939.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="PNS21_002248"
     CDS             complement(2997..3524)
                     /locus_tag="PNS21_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159940.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="PNS21_002249"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="PNS21_002249"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDI5159941.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="PNS21_002250"
     CDS             complement(4401..4886)
                     /locus_tag="PNS21_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDI5159942.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="PNS21_002251"
     CDS             complement(5039..5236)
                     /locus_tag="PNS21_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159943.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="PNS21_002252"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="PNS21_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HDI5159944.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="PNS21_002253"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="PNS21_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDI5159945.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="PNS21_002254"
     CDS             9211..9423
                     /locus_tag="PNS21_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDI5159946.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="PNS21_002255"
     CDS             9425..11104
                     /locus_tag="PNS21_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDI5159947.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="PNS21_002256"
     CDS             complement(11358..11558)
                     /locus_tag="PNS21_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDI5159948.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="PNS21_002257"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="PNS21_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
BASE COUNT         3589 a         2654 c         2132 g         3934 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgatatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaattatg taccctgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc
    12301 tgaccaact
//
LOCUS       DAMYXM010000070        11735 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.073, whole genome shotgun sequence.
ACCESSION   DAMYXM010000070 DAMYXM010000000
VERSION     DAMYXM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.073"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(368..1486)
                     /locus_tag="PNS21_002258"
     CDS             complement(368..1486)
                     /locus_tag="PNS21_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159949.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     NVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1957..3264
                     /locus_tag="PNS21_002259"
     CDS             1957..3264
                     /locus_tag="PNS21_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDI5159950.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3383..4582
                     /locus_tag="PNS21_002260"
     CDS             3383..4582
                     /locus_tag="PNS21_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDI5159951.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4609..5877)
                     /locus_tag="PNS21_002261"
     CDS             complement(4609..5877)
                     /locus_tag="PNS21_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HDI5159952.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6040..6686)
                     /locus_tag="PNS21_002262"
                     /pseudo
     CDS             complement(6040..6686)
                     /locus_tag="PNS21_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7094..7597)
                     /locus_tag="PNS21_002263"
     CDS             complement(7094..7597)
                     /locus_tag="PNS21_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDI5159953.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7720..8484)
                     /locus_tag="PNS21_002264"
     CDS             complement(7720..8484)
                     /locus_tag="PNS21_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDI5159954.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8592..8867
                     /locus_tag="PNS21_002265"
     CDS             8592..8867
                     /locus_tag="PNS21_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDI5159955.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9305..10255
                     /gene="yidC"
                     /locus_tag="PNS21_002266"
     CDS             9305..10255
                     /gene="yidC"
                     /locus_tag="PNS21_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDI5159956.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10434..10634)
                     /locus_tag="PNS21_002267"
     CDS             complement(10434..10634)
                     /locus_tag="PNS21_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159957.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
     gene            11439..>11735
                     /locus_tag="PNS21_002268"
     CDS             11439..>11735
                     /locus_tag="PNS21_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5159958.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKER"
BASE COUNT         3560 a         2448 c         2076 g         3651 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcagcactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacattaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa
     1561 taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa
     1621 gttatcttca taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg
     1681 ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
     1741 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
     1801 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc
     1861 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
     1921 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
     1981 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
     2041 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
     2101 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
     2161 atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat
     2221 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
     2281 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
     2341 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga agctaaattc
     2401 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac aatcgttaat
     2461 gcatcaggat tgaacaactc ttatcttggc gaaaaccgac cagaaggcac tggagaaaac
     2521 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
     2581 ttcccagaaa tactggatgt atcaagtact acgacccaaa tgtttggcga aaatacgcaa
     2641 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
     2701 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
     2761 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
     2821 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
     2881 tcagaaaaag agctcggaaa agctaacgca agtattccta gtctaaaaac cattgatgta
     2941 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
     3001 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
     3061 actgacaatg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
     3121 accttggcag acgataaatt aggctattta gaagacaaca atgcaccatc aacagagatc
     3181 attacggata aaacagtgga aaaagcgaat atattcgtga ttggctggcg acgcgtcgca
     3241 gaatttttcg gcaacttgtt ttaattgaac aataagaaaa acatctttcc atcggaaaga
     3301 tgtttttttg ctttttctag tttaaataac agcttttttc atctattttc gttatactgt
     3361 ataagaaaaa ggagttgaat ttatgaatct aaccagagat ctgatctttt tagttctcta
     3421 tcttattggg gtattctcta tcgtttattg gcgaaaacag aagaaggatc cttccttgtt
     3481 cttttacggc agtacgtgga catttacggt aatcttaact tattttgtcg tgttggaaat
     3541 tttttattcg accttttatt ttttgattcc attgttgttc tttagtatct ttgctttttc
     3601 ttatttcaca gaaaaacgaa ggctactgaa tggtctttta ttcaatgttt tactcatcag
     3661 tttcggcatc tacttgtttg tcttgctgta tgaaacgcaa aatatttttc ttggcggact
     3721 gatcgccttg atcacgattc cattgctact tgtcctttta tttggtattt atggattgat
     3781 tgtttttctt ttttggaacg gggtcacagt tttacgtaga gaatcgcatt cattagctaa
     3841 tcttttaacg ttgatactag ccatttttct tacactgttt cttgtttttg actttttctt
     3901 gttaaaatat ttgccgcaat ggataaatgc tttgtttttc tgcgttcccc tgatcttgat
     3961 ctatctattt atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg
     4021 gcctcgatac aatcaagact tcatcgttgt gttaggcgct ggattgatca atggagaaac
     4081 agtttctcct cttctagcca agcgaatcaa taaggcaatt gctttttatc gggctcaaag
     4141 ccgtgctaca ttaaatccgc ccatcttatt gatgtctggc gggcaaggag cagacgaaaa
     4201 agtaccagag gccattgcga tgaaacaata tgccatggaa caaggaatcc ctgaaagaga
     4261 cattcttgtc gaaaccaatt cgacaaccac tttggaaaac atgctctatt caaaggaaat
     4321 catggatcag caaatgaaag ggccataccg cgcgatcttt agttccaaca attaccacat
     4381 ttttagagcc ggtctttatg cgcggcaagc caaattgaaa gcaaatggta ttggtgcaaa
     4441 aacagctttc tactacttgc ctaatgcgtt tttaagagaa tacctcgcca tcttgatgat
     4501 gaacaaaaaa agacatatga tcatttgcgg attgcttttc gcaggctccg cattcttatc
     4561 gctgattact ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt
     4621 tttttctttc attctcttaa ctattggcaa aaagagtatc ccttttttga ccgctacttg
     4681 ctgagcagaa taaataccgc tgttgcctga acacataaag ctgatcaagc agatcataaa
     4741 gaagtatact gccgcttgtg aaccgaacaa ttcgatcccc atcacaaagc aggcaatcgg
     4801 tgtatttgtc gcaccagaaa atactccgat aaagcccaat cctgcaagaa aaggaacaga
     4861 aacatggagc aaaggcgcta gtgctgcacc aagcgttgca ccaatttcaa acaatggtgt
     4921 cacttctccc ccttgatatc ccgcgcctaa agacaaaact gtgaagaata acttcccaat
     4981 aaaatcatag acatgtgcct gtccattaaa tgattcttga agtaacggaa gactcaatcc
     5041 gagataacgt tgtgtttgca aaatcaatgc tgctgccaca actacagctc cacctatcgt
     5101 atttcgacaa acaggatgag gaatccattg tgcatatcgc gcttttaaaa agacgatcga
     5161 acggctgaat acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa
     5221 cagcaacata ctccattcag gaatcacacc catttggtaa tgtagatgag aaacaccata
     5281 gctttctgtc atgaaatttg caaacagtgc agcaaagaag ctagggaaga tcgcttctgt
     5341 tcggactttc ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc
     5401 aaatacagaa ctaaaacccg cactgattcc actgataatc aagatttccc gttctgattt
     5461 atccaatcgg aacaaacgtg ccgtattatc tgctaacgct ccccccattt ggactgctgt
     5521 tccttcacgg ccaacagatc ccccaaataa atgggtagtt atggttccaa acagtgtaag
     5581 tggaataagc cgcaacggga ttttttcttc tccaccattt ccttgatcga tcactaagtt
     5641 gtttccacga ctagaaagtc cgccgtgata cgcatagaga taagcaaaaa gcgcaccact
     5701 aactggtagt agacaaagga gccaaggatg tgatagtcgg atatctgtca caactgtcag
     5761 gctatttaag aaaaaagcag acaagctccc catgaatata ccgagaatcg ctgtgatcac
     5821 cagccacttc agcatataca ctaaaatgcg tactggactc ggtaattgtt tttccataaa
     5881 aaattcctcc tcaataaaaa aacgcctact aaagaaaggg gctgtcccca gactttagta
     5941 ggcgtcatta gctatctttc gttagcattt aaaggcgaac accatcacct attcacttcc
     6001 attaaaagat agcatatttt tctgtaaaaa acaataaaat tatcgttctt tctttacacg
     6061 ttcagcaaat tcatagaaag gagccaattt cgttaactgt tgattacatt ggtcactatc
     6121 atgacaaata taattcccct ttttcgtata cgtgccatca gaacctgctt tcgttgtaga
     6181 aagaaaaagc gaaacattcg atgttttctg acagattgca cagattccct tatttatcgt
     6241 tggtgataaa gtaccgctaa tcccttttaa tttttcatct tcgtaataga ccatgaattt
     6301 tttctgcgta cctggatcat tccagccgat gtacgtatgt tcttgcaagt cgatttcatt
     6361 ccaagctggt tgtttgagtt ttttcacttt tcggaataat ttctcgattt gtggtttgct
     6421 aggctgcata aacggctgaa cgatttgctt cagctcctcc aatgctcttt ctgcttgcgc
     6481 ttttgtcagt tttacgtcta aaactgtcgt taaaaatgta tcgattctgg cagttcgtga
     6541 tctaatatgt ctcgaatctt ttcagctgct agtgcctgaa ctgtttgtac cgtttttgga
     6601 tcattgacag atgagtacgc atttaataac tgttctactt ctcttattat aaaaaaatat
     6661 tggtatggtt ttattgtttg ttccatgatt ttaaagtcct ttcttctaac caaagtgcat
     6721 actttgtaga aaaaagtgat cttctgcaaa gtatgtgtta cctgaagatc ccggactctt
     6781 ttaaataaga tacgttttgc atcttatcat cacccccttt aatgcttatt attctatgta
     6841 catgattcat tcgataaata aaagtaaacc gtatcttgaa cagatagtca actgcaaaca
     6901 gaaaaaagga ctgtgacaaa agtcgtgtca aagtcccttg tttcgaatga atggtgttca
     6961 aaagctggca tctgcggtaa taagccgaaa cttcacaaaa atttgagaag caattttcgt
     7021 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctcaata tggtctatta
     7081 ttggttttat ctattaaatc gaggattctt tcccaaagta acgttgccat ctcttacgca
     7141 tacttgctga cataggctga gcggcttctt tgactgcgca gtatttgtct acgaatgtta
     7201 cgatatatcc ttctttgtac ttaggcggtg caatcgttgc gccccacatg tgcttcaaaa
     7261 tgatatctcg ctcaagatcc gacaattcgg tgatcttttc tgcattcttt acagcaattc
     7321 ttggatggat atacgcatgt gtgccttcat caaatttcgt cgtacgccaa tcatagtaga
     7381 acagatcatg caaaagtcca gcacgcgctg ttgctttagc attgccaccc catttttttg
     7441 cgagcagata actatagtaa gaaacactga tggaatgctc caaacgagtg gaatgaacat
     7501 gctgtgtgta attcgctaat tttttcactg cttctgtttc taaaagatct tctacataag
     7561 acatatattc ttcgtcttca cgccattttt ctgtcataat tatcgatctg ccaccttttt
     7621 gaatttcatt ttcttctgaa attgatctaa gtataccacc agtgttcgga ttattctagt
     7681 cgccttggat aaaatagcca aagcttaata gttccttaat cataaagaaa aatgataaag
     7741 aacaatccct gccgcaaccc caacattcaa cgattccgca tttcctttca tcgggatata
     7801 tacgttctga tcagtcaaag acaagatttc ctgacttacc ccctgacctt cgttccccat
     7861 gattattgcg aagttatctg atttttctac tttgtctaat gcgtgtgctt cttcatttaa
     7921 ttctgtgcca tacactgtaa accctttgtc tttcattaaa gaaatcactt ctgtcaaatc
     7981 tttttgcaac acaggcaaat gataattgct tccctgcatg ctgcgcagca cctttgtact
     8041 ataaatatct gccgttcctt tgcctaataa aacaccagcc atatcaaatg catctgccgt
     8101 acggatcatc gtccctacat ttccaggatc ttgtacatta tctaacaaca gccatcctcc
     8161 tgaaaaatct ggcgtgcctt cagccgttaa cgccatcact gctaagattc cttgtggtgt
     8221 aggtagatcc gatacagcat ccatgacctc atccgaaact agaaaagtag aaatctcttt
     8281 gtttgcggcc agccagtctc cccattcttt catgcctcgc tgtgtcacaa aaagttcttt
     8341 tatttctgca tcagacgacg cagcttcttc aatcagatgg aacccttcca aaagatattc
     8401 ttccttttct tcccgatatt tctttttatg tagttttttt aattctttga ttcttggatt
     8461 ttttgctgat tggatctctt tcacattcct cacctcccca tgattatatc atggaatagg
     8521 tgcttgagct tgtttcatcc taaaaaaatc ggtatcatta atttataaaa taggaaagaa
     8581 aggtgacgaa catgcgcaaa gttaaaatga acgtccaagg acgcgtccaa ggcgtaggat
     8641 ttcgctacat gaccaagatg gtcgctgatc aactaggcgt gactggaacg gtaaaaaacg
     8701 aagatgacgg ctcagtcacg atcgaagcaa tcggcaatga cgacatcatc cagaaattta
     8761 ttgaagaagt caagaaatct ccgagcccaa gtggacgagt gcagtatgtg gatgtccaag
     8821 aagacccgat gattgaggaa cgaaagaaat ttgatgtggt gggataaggt tgtgaaaaag
     8881 ctggtctgca tcaagcatta aggacaggtt gtgaaaaata gctctccata tttttcaaca
     8941 ggctggactt aatgccgtag atgcaagctt ttgaacaccg tcgataaggt tattcttgag
     9001 gcgctttgcc tcaaaaatta aggacagcag tgaaaaagct gatctatcac ttgcagtgag
     9061 ttcatttgac tccgcttttt cgggcagaaa aaaagttttt agtttattaa agtaagaaag
     9121 aaacacttgg aatcattcta ctttttccta gtatttcttt cttgattttt tgtctttttt
     9181 tcaaaatttt tcgctacgta aaagaacgaa tatttatatg aaatttctat gaagttgatt
     9241 gaattccatg cgctttttta gtatagttaa gtttgtgtaa aaattataga agaggaagta
     9301 ttctatgaat aaatggaaaa actggttatt gggatccgga cttgtcagta tcctgctctt
     9361 tttatctggc tgtgtgcgga tggattcaaa cggaaaccct gacacttcgg gaatcgttta
     9421 tcgagtactc gtccatccta tgggccaagc gattacatat ctagttgaaa atttcaattg
     9481 gtcttatggc tgggcagtta ttgcaatgac tgtgatcgtc cgtatcatca tcttgccact
     9541 aggtattagt caatcgaaaa aaacaatgat ccaatctgaa aaaatgcaag cgttaaaacc
     9601 acaagttgaa gcagcccaac aaaaattgaa agctgcaaca acacgtgaag aacaaatggc
     9661 agcacaagca gaaatgcagc aagtataccg cgaaaacgga ttgagtatga ccggcggaat
     9721 cggctgtctg cctctattga tccaaatgcc gatcttctct gccttgtact ttacggcacg
     9781 ttatacagaa ggtatccggg aatcatcttt ctacggcatc gatttaggct caccaagtat
     9841 ggtacttgtt gccattgcag gggtcgctta tctgcttcaa ggatacatct caacgatcgg
     9901 tattcctgaa gaacagaaaa agacgatgcg tacgatgctg atcgtctctc ctgctatgat
     9961 cgtcttcatg tcgatcagtg cgccagccgg tgttacactt tactgggtag tcggtggtgt
    10021 gttcagctgt ctgcaaacat tcatcacaaa cgttatgatg aaaccacgtt tgaaagcaca
    10081 agtggcagaa gagttgaaac aaaacccacc aaaacaagtc gtcactccaa gaattgatgt
    10141 gacagaatca gctaaaccag ctgacagcaa acaagcaacc aaacaaatca agcaacccaa
    10201 aaacagtaca aacaataaag gccgcaacgc aggaaagcag cagaagcgga agtagatagt
    10261 aaataagagt gtaataaaaa ttcattaaca gttttagcaa ttaaaaatat cgaccaagat
    10321 ttttgaaaaa gaaatctggt cgatattttt atttaagtga atgtttaatt tgaatcatcg
    10381 attgatcgct atctaattgc agcagcatct ttttatgaag aaacaacttt ttcttatcta
    10441 tcaacgtaga caggtgttgc tgtttcttct aatagtgtac ttaattcctt aatattttgt
    10501 tttccttctt ctaacatcca actactcgct ttttcttcgc cttcggtaac aaaaggcgcc
    10561 actcccttcg cccatgttgc tcgcccacat aagacaccat tctccataaa ggacaacgcg
    10621 gagataccac ccatctaaat aacatccata aacctttcta gtagaagaat agtgatagta
    10681 tccatccctt tttctactgg gaatacaatt acacgattca aagcttgata cacaacattt
    10741 cccttcttat aaaccgctat tgtaaccgaa ttgttttgaa tcaccttttt gggatagaaa
    10801 ctggctcctt tccgcaactt cttaaatgtt ggtttctttt atactttctt tgtagcaact
    10861 acaaatttct tcaaatctct aaatgtttgc ataacaaccg cctttctgac ttttactcag
    10921 gatggtattt aaaaatcagc aaaataaggc ttctctccat cttttttatt tcctagctgc
    10981 tttaaagaca ctcttgctgt tttttaatct ctatcaaggg ggctctgaat ctattttgat
    11041 gaacttatct tattaatttc tcaaatgagc tgtcagacac aactgtattt tttcaatctc
    11101 ataccggaca actatgccag cttaaaaaaa tttcctctgg caaaacttct ttattgaaaa
    11161 atgacaatga aaaaagacca ttctttttgt tgaatgctca ttgtgtaaac atatttcctg
    11221 cgactgctct tcgaaccatt ctcagtgcaa tccctaattt atctattaaa tcttctttta
    11281 gttcctcatc tttcatattc aaaatttcct ttgtatgtta cacaagtttg ttctctaaat
    11341 aacgcgaatt gtaaaattaa gttagaaaaa taaaaaggca tttatggtac actcaaattg
    11401 tatttccgac gaaagaaaac aaaggagtgt aaccataaat gacctacaaa catcttacca
    11461 tagacgaact gacaatgata gaatcatatt atcttcaaca taataagccg gttgaaatcg
    11521 ctaaccgaat gggacgtgct atacaaacta tttataatgt agtcaataag ttcaagcaag
    11581 gtaagactgc tcttgattat tggcaccagt ataaagaaaa taagaaaaaa tgtggtagaa
    11641 aagtcattca attacctgct catgaagtag attacattaa agagaaagtc actcttggtt
    11701 ggacgcctga cgtcattatc gggcgaaaag aaagg
//
LOCUS       DAMYXM010000071        11655 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.074, whole genome shotgun sequence.
ACCESSION   DAMYXM010000071 DAMYXM010000000
VERSION     DAMYXM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.074"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..1419
                     /locus_tag="PNS21_002269"
     CDS             <1..1419
                     /locus_tag="PNS21_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDI5159959.1"
                     /translation="LADGQKGDNRLKTLKEIQQIYPQIPESKGGFPGYGGIPITHYKK
                     YWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAERQSSMVVNDPKTELYVASKDE
                     LEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDIDTATQLTNTFTNTIYYDANAS
                     DNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNNLVELLSEIGGFNYQDPDNEFK
                     QLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNILSTAITGLNPFTLTKIAKMTSQ
                     STIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKKFHEERVKVGYRGFCEINFDCH
                     LEQGDHVCLKYDHRGKEYVAWYEFSQRVLKDEEGNIVYRKKRGETHLPELEKEAVLTL
                     DEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHIIVSIFLSQLYKELSTQCVKTKG
                     DKCHRRIHFILDEFGNSVTCLA"
     gene            2132..4030
                     /gene="ltrA"
                     /locus_tag="PNS21_002270"
     CDS             2132..4030
                     /gene="ltrA"
                     /locus_tag="PNS21_002270"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDI5159960.1"
                     /translation="MTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSNFKNLMK
                     FIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKDYRPRKV
                     KRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNCSTENAI
                     ADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRKMLKAPI
                     VMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHYRKNGAW
                     DKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERLKLPISM
                     EKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIKKIQYAG
                     AKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARGMTKNGI
                     YGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRKLIHQEQ
                     QAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEIECHHIN
                     PYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTYRKKAAN
                     FPISIKMN"
     gene            4147..4947
                     /locus_tag="PNS21_002271"
                     /pseudo
     CDS             4147..4947
                     /locus_tag="PNS21_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            4944..7316
                     /locus_tag="PNS21_002272"
     CDS             4944..7316
                     /locus_tag="PNS21_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159961.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLENDPNKVEHIPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNVAKNVGSGAKNLASAGKSGLSNLNDIRKNGFGN
                     RKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTAE
                     DLTEKAKDTLEEKLDGTRAKELAEAVKNPSETLEKQKEKASEVKEGLKDAYGEGQVNA
                     AVKANTTTEKENQEGAAIPSAKDHQDELHSNENSEEGQNGQLPKTDPEQYRYKSNINS
                     ENEKRISDLPKRNQEVQTDDDSIDLPNDNGVEVGTEKSTKIGLPKAQNLPMKDSRLNQ
                     FKENDQSFVHQSTAGNVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAP
                     EPIQRVHTGYKPANVAFTAPTKINVTRETKNRNNELKEQLLRDIKQDIKP"
     gene            7368..7700
                     /locus_tag="PNS21_002273"
     CDS             7368..7700
                     /locus_tag="PNS21_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159962.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            7701..8294
                     /locus_tag="PNS21_002274"
     CDS             7701..8294
                     /locus_tag="PNS21_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159963.1"
                     /translation="MRVKGQNSVDKKSIFQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFIIEGRTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            8457..10481
                     /locus_tag="PNS21_002275"
     CDS             8457..10481
                     /locus_tag="PNS21_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDI5159964.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIKNLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            10481..>11655
                     /locus_tag="PNS21_002276"
     CDS             10481..>11655
                     /locus_tag="PNS21_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDI5159965.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMNF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAMKHWGEGGNDNGTITGEWTNPFPGSSLDKSTFSGGQLFGTNPGS
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     "
BASE COUNT         4161 a         1880 c         2436 g         3178 t
ORIGIN      
        1 ctagcagatg ggcaaaaagg agataaccga ttaaaaacgt taaaggaaat ccaacaaatt
       61 tatccgcaaa taccagaatc aaagggaggt tttcctggtt atggcggaat accaattact
      121 cattataaaa agtactggta tattcagacg gatacagtaa atacttgtat tgttggtact
      181 tcccgttcag ggaaaggaca gatagaagtt ttagcaacca ttgacaattt gtctcgtgca
      241 gaaagacaaa gttcaatggt tgttaatgat ccaaaaacag aattatatgt tgctagtaaa
      301 gatgaactag aagctagagg gtatgatgtg tatgcattta atatcaccga tccactgcaa
      361 tcaatgagtg ataatccatt ggcactcatc gtgaaatact ggaagcgagg agacatagac
      421 acagcgacac agttaaccaa tactttcacg aatacgattt attatgatgc caacgcttca
      481 gacaataaat actttaacga aaatgcgcaa aaagctgtca atgcgcttat ttttgcgtta
      541 ttggaacaag cggatcagac aggggattac tccaaattaa caccgaataa tctcgtcgaa
      601 cttctttctg agattggtgg ttttaattac caagatcctg ataatgaatt caagcagctc
      661 aatgctttag atgaatttat gaatcagtta ccacctggaa acatagcaaa aaaacaatat
      721 ggggcaacaa aaattggttc tgataaagcg aaaggcaaca ttctatcgac agcgatcaca
      781 ggactaaatc catttacact gacgaaaatt gcgaaaatga cttctcaatc aaccattgat
      841 tataagtcca ttggttttcc gaagtatttg gatctcaaac tacacgagag tctgttaaac
      901 cagcgaattg ttgtagagtt ttatcatcaa ggaaaaaaat ttcatgagga acgtgtcaaa
      961 gtaggctata gaggtttttg tgaaattaac tttgattgtc atttagaaca gggagatcat
     1021 gtttgcttaa aatacgatca tcgaggaaaa gaatatgttg cttggtatga gttttctcaa
     1081 agagtattga aagacgaaga gggaaatatc gtttatcgaa aaaaacgagg agaaacccat
     1141 ttgccagaat tagaaaaaga agccgttcta acattagatg aggataaaag taacttatta
     1201 gtagagtcta ttcggatgca ctataccgat aaaccaatgg ctatttttat gctcacacct
     1261 gattatgatc catctaacca tatcattgtt agtatttttc ttagtcagtt gtataaagaa
     1321 ttatctacgc aatgtgttaa gactaaagga gataaatgtc accgtaggat tcattttatt
     1381 ttagatgaat ttgggaatag tgtgacctgt ttagcctaag gataaaagag gctagttcca
     1441 acgaacattg gaataacttt actatcgaga aatcaaatga cggggtaacg ccctgaaggg
     1501 tttccactga tactgcgaca tgcgttagta ggatgtgcat ccaaacgcgt atgaagctcg
     1561 ctgaagtcgg cagaagggta ccgtaaggaa agacacgata cgtatctacc acgcaagtgc
     1621 tctagaggta gggtatgtac ctgatatgtc gggtgtccta aaaagttcaa taaggtgaga
     1681 atgatttgat tatctgacaa acttgcgaat ggaatgtctg aaacgaataa ctcatagaaa
     1741 tgtgaggggg aagggggata cgtcctcttt ccattgactc gaggttgagt aaaaatgtgt
     1801 ttctatgaaa aaccttgcat ggttaaaggc tatggagtag tcaacaggca aaagcaagca
     1861 cctaagttga aatctatgga tagatagtaa ggaacagggg aagcatctag cacggaggga
     1921 gcataccgaa gatgtgtttc taaggaaaaa ccgaaatcgg ttataactta ggtgaataga
     1981 tgtgaaagta gagccacgac cagtgaaaat ttcgtaatga aattggagga acaggctcaa
     2041 gtcgttattt ataatataag aaacctattt aaaatcatgg attctcgtaa gactaaaaag
     2101 gtcacgaacc agaatggagc gtgataaaac catgactaac acaaaaggaa ttagcttaaa
     2161 gaacaaacaa ctcagacacg ccgaatacta cgacatgact gagactttcg atgaattgca
     2221 cgcaatgagt gttgcaaaat caaattttaa aaatctcatg aaattcatta ttgatgacaa
     2281 taatatcttt ctagcgtata gaaatatcaa aaagaataaa ggaagtgcaa cggccggata
     2341 cgatggtgtc actataaaag acattgaaaa attggcatgt ggtgactttt taactattat
     2401 taaaaggcga tttaaggatt acagaccaag aaaagttaaa agagtagaga tcccgaaacc
     2461 aaacggaaaa cttcgacctt taggaattcc ttcaatatgg gacaggatag tccagcaatg
     2521 tattctacaa attttagaac caatatgcga agcaaaattt aataagcata gctatggatt
     2581 cagaccaaat tgttctacag aaaacgccat agcagattgt cttgctagaa tcaatcaaag
     2641 taagatgaat tatgttgtag atattgatat aaaaggattc tttgacgaag taaaccatac
     2701 caaacttatg cgtcaaatat ggacaatggg gattcgagat aaacaactcc ttgtaattat
     2761 tcgtaagatg ttaaaggctc cgattgttat gcgaaatggc aatgttgttt ttccaaccaa
     2821 gggtactcca caaggaggga ttttaagtcc actgcttgcg aatatatgcc tgaatgaatt
     2881 tgattggtgg atagctaatc aatgggagga acgaactgtc aaagagctga aacaccacta
     2941 tcgaaaaaac ggagcatggg ataaaggaaa tatctattcg aaattaagaa gccacacaag
     3001 tttaaaagag atgtatctgg tacgctatgc agatgatttt aaaattttct gccgaacagg
     3061 agaagaagcc caaagggctt tttatgccac acaaaaatgg ctcagagaaa gattaaagct
     3121 cccaatttca atggagaaat ctaaagttac gaacctaaat aaagctgaaa gtgaattctt
     3181 gggtttttca ataagactcc acaaaaaggg aaaaaagaaa gtggctttca cgtacgtttg
     3241 tcgcaaagca gaagaaaaca ttaaaaataa actaaaacaa cagattaaaa aaattcaata
     3301 cgctggagca aaaaaaaata ggcaccgagc aataggtgac tacaacatga tggtcatcgg
     3361 tatccacaat tactatcgga tagcttcaca ttgtagtgtc aatttcactg agatttatag
     3421 aagtgttagg acagtaatag aagcaagact gaaagctaga ggaatgacga aaaatggaat
     3481 ctatggacaa gatcatgaag gataccgtcc gtatctaaag tctaagcaaa tgagatatat
     3541 tgaaggtttt ccgatacttc caattggcta tattaaaaaa agaactccac tcaacaagcg
     3601 gagaaatcta aataaatata ccaaggaagg gagaaagctc attcatcaag agcaacaagc
     3661 tatagaaagc tggaaaatca agtatcttgt aaaaggtttt agtaacaata agaaatacac
     3721 ggtggaattt tctgataaca aaatatctaa gtttatttcc caaaaaggga aatgcgctat
     3781 cacaggaaac gaacttttga tggaagaaat tgaatgccat cacattaacc catatcattt
     3841 gtccaaagac gatagttaca agaaccttgt cattatctct agagaaatcc atagattaat
     3901 ccatgcaact agtgaagaaa ctattctgaa actgcttcgg ttactgaaac taaatcacta
     3961 tcaaattaga aaactaaaca cgtatcgaaa aaaagcagct aattttccaa tatcaatcaa
     4021 aatgaattag ataggaaaca aattatattg taattaacga tggaacgccg tgtgcggtga
     4081 aagtcgcacg cacggtgtgg accgggggaa aagacggaga taatatcaaa ctcttaccta
     4141 tcggaattgc caccaatgga taatatggaa ggaatcatga cagtcacagc tggtagaaac
     4201 atgttgtggg atttgtttat ccaatcctat cagcaacttt atgcgaagta tggggaacaa
     4261 aacgcagcga ttatcaaaga aaattgtcag acacatattt atattatgtc gacagacgat
     4321 aataccatag aagaattttc caaaaaagtg gggaataaga cggttgaaca agagaacagt
     4381 accatcaatt cattaggtat gaatactcat atcaatcgca atgttgattc agatcgcatc
     4441 ttaactcctg aaagaatttc tactttttta gaaggtgaaa cgattgtact cgctccttta
     4501 aaacgacaag atttgaaagg tcataaaata cggccgtatc ctatttttaa tcatggggaa
     4561 acgaatcttc cttatgctta tcaatatatt gaagatttca atccatcgtg ggatatcaat
     4621 gatatagaca tacattgtac acatgcggac ttggatctta aggctttaca aattgattac
     4681 gattattttc tacctgatat ctatgcacgt cgactttatc acaatatgca cgatcaaata
     4741 gaagaggaag ctaaagaaaa catttttatt aaaatcgttc aatctttaga caactcaacg
     4801 gcacaaaagt tacttattca attttatgaa gagaagcaaa tagatcgttt gcaagaatac
     4861 cttgaaaaat tgcgtcaaaa tgaagaaatt acagaggtta taaggaaacg acttttgcta
     4921 gaagctgaaa aggcggtgaa agaatgatca ttggaggaat attttccggc attgccgact
     4981 ggtggtcagg cttatttggg gatggggatg cagatcccaa aaccgtaacg aaagtcttac
     5041 aagattatgg taattatctg cagtatcaag atgtattcag ttggatttgt aatacttttt
     5101 tatggggttt gattaaaggg ttatataatc tcaatacttt actagaaaaa acaatttatc
     5161 aaagctttga tttaaaagac ttattgaacg ccgctggtgt taatgaactg tttcaaagtt
     5221 tgattagtaa ggtattggct cttttttgtg tcgtcacatt aatttattta ggcttaaaat
     5281 tttttatgag taaacatcca ccaaaaatca aaaatatctt ggttaatcta tttatggcca
     5341 tgattttgat ttttggtggt agttctttaa tcgatgaagg gctaaatatt agtaaaaact
     5401 tttacggaga cgtgactgca gcaaataaag cagataaaac tggttcacct gcttttcaaa
     5461 ttattcaaaa taatgtgatt gatgtgagta cgttactaga aaatgatccg aacaaagtag
     5521 aacacatacc aacagacaaa agaaatgctc tcacggcgaa aaattttaaa acagcgaata
     5581 tcaatgggat tattacacct gatcaagcta agaaaatgtc tgaatcaagt gatattagta
     5641 cagtacaaaa agatcggtta aaggcattac agtatcgact agagttagac gataatggga
     5701 aagaaatccc tgttcaaatt agtgatgaag gtttagcaaa atatgtgtat acttcaggct
     5761 atcgccgata cattagtagt ccaggtatta ttttagctgg tgaatcttct ttgtcggtag
     5821 cctatttatt tatcttgttt acgattgtta cttgcattat tgagctggta tttaaaaaat
     5881 tctatttagt gattgccgcg tcaacggact ttgagacagg acaacggatg aaaagtgcta
     5941 tagaagatat ttctcaatcc ttcttgttat tggcatttac gattttagaa ttacgaatct
     6001 atacattaat gctttcaggt atcggtgatt tgcatgcggc aggaaagatc aatggattct
     6061 tgtatgttgt cgctttaatt gtattaacca ttgcgctatt caaaggctct caatcagtta
     6121 cgaaaatttt cggcgttgat acgagtctga aaaacggcag caattctctt cttagcatgt
     6181 ttgccttagg aaatgttgcg aaaaatgtgg gtagcggggc taaaaacctt gcaagtgctg
     6241 gtaagagtgg tttaagcaat ttaaatgata ttcgtaaaaa tggatttggt aatcgaaaag
     6301 atgttacccc tgataacggg gaagaacaat cagacgataa agctacaact gtacctaata
     6361 gtaagaaaac agtttccgaa atgttcggga aagcgaaaaa tggtgtttct aaagccgcag
     6421 aaggtcttgg ttacgtcaat gaacgtgggt taaaaggaac agctgaagat cttacagaaa
     6481 aagccaaaga caccttagaa gagaaattag acggcacgag agctaaagaa ttggctgaag
     6541 ctgtgaagaa tccatccgaa acactagaaa aacaaaaaga aaaagcttca gaagtcaaag
     6601 aaggactaaa agatgcgtat ggtgaaggac aagtcaatgc agcagtaaaa gctaatacaa
     6661 caaccgaaaa agaaaatcaa gaaggtgcag cgattccttc agccaaagat caccaagatg
     6721 agttgcattc taacgaaaat tcagaagaag gacaaaatgg tcaattacca aaaacagatc
     6781 cagaacaata tcgttataaa tctaacatta attctgaaaa tgaaaagcgc atttcagatt
     6841 taccaaaacg aaatcaagaa gtacaaacgg atgatgattc catcgatctg ccaaatgata
     6901 atggggtcga agttggaact gaaaaatcaa cgaaaattgg tttacctaag gcacaaaatt
     6961 taccaatgaa agatagtcgt ttgaatcaat tcaaggagaa tgatcaaagc tttgttcacc
     7021 aatcaactgc aggtaatgtt caaatgaaac caagtcgttt gcctgaaaat ggaaatctga
     7081 caactgtttc ttacaattta tctgaaagtg gggagacagg gaatgcgtcc gttcaaaatg
     7141 ttgtgacaag agaagctgca ccagaaccta ttcaaagagt tcacacaggt tacaaaccag
     7201 caaatgtggc atttactgca ccaacaaaaa taaatgtgac aagagaaact aaaaacagga
     7261 acaatgaatt gaaagaacaa ctattgaggg atatcaaaca ggatatcaaa ccataaaagg
     7321 cctacaagtt aggccttttt tctatgtatg aaaaggagaa atcatttatg gataaatata
     7381 ttaacatgaa aaatcctcca agatttagtg gggaatggaa gttttttgcc tttttctttt
     7441 tggatctatt aacgcttggt ggttgctttt tttgggcgaa aatagtcaat aaccttgtcc
     7501 atttaccact cttttttgca ctcttgaatt atggcttatg gattgcgata gctctagtga
     7561 ttgtttggcg accctataat aacccaggtc aacgacaaat ttttgcgatt atcgagtcta
     7621 ttttttttac ggacaagaac cattatcatg cgttagatcc taaccgaaat tatcatggta
     7681 cgtataagga ggatgaataa atgcgagtca aaggacaaaa ttcagttgat aagaaatcca
     7741 tttttcagtt tggaaaacca gaagaacgat ctatcttgga tttatttcct tataaatcct
     7801 gtacagaaga gggagtctta gttactaaag aagatcattt tcaacgttat tatcgtgtag
     7861 tttctacaga cgtggaagga ttaaatgaac aagagaaact agaacgaatg aatcagttga
     7921 ccaccgtcat gcgtacgtat gtgccaacaa ttaagttagt tagtttgacc acagagacgg
     7981 atttaacgga acaaatcgta caaaagcggc agttattaga taagaatcgt atggaacaat
     8041 ccttaggaag aaatctacag cgattaagaa aatatgaaaa gaaattagta gaggaaatcc
     8101 aggaattgaa acgagcagaa caagaacgtc ctgacttaag cttctttttc atcattgaag
     8161 gaaggacaat caaggattta caaaccagaa accgtcagtt aatgcgatct agtggcattt
     8221 tagggttaaa accactctac aaaaaagaat tgatcaagat tctgtatcga atgaacaaca
     8281 tgaatgatga gtaggaatgg agaagagaga atgaaatatc cgttgatatg gttagaaaaa
     8341 gatgttccga aggaagagac cactagaaaa tttcctcaca ctttaagcct gggtaagttg
     8401 ggaaaagggc aatcttttca ccaagtaaag aatcaaacaa gaagaggtga ctgtacatgt
     8461 tagaagcgat taaaacagct ttttcaccac aaaatgatca aaaagtactt gatccattaa
     8521 agcaactagg aaatcgggaa atagaaattt taaaatccaa aggatatgat ttagattttt
     8581 taaaacaagt gatgccacaa ggtggattaa atttcgatga agagtatgtg acctatggaa
     8641 atggttattc acggtgtgtg acagtttatc actacacaaa aaatcctact cttttttggt
     8701 tggccaatct catgaacaat cctcatacag tggccacact agatgttagc acagatgaaa
     8761 agcaaaaagt gattgatcaa atcaatcgcg cattagatga attaaatgat cgatcagata
     8821 atgaacgaaa agcgactgac cgaaataaaa gtgcggaaga atacctggag ctagcggaat
     8881 atgcccgaaa attgacttct aatggggaaa tttctaaaca agtcaaagtg agagtacatg
     8941 tttacggaaa cacgattcaa gaagtagatg aacgggcgca ggaactgatc aaaaatctaa
     9001 aagggatgga ttatcgtgcg gtgacttatc tgcataaaac caaacgagat tggcaatcga
     9061 tgtttaccat gttcgatgaa caagctgaat ggttcggaaa tatcaaaggg caatcactac
     9121 catcgttaaa cattggcgga ggtttcccgt tccaccacca aacgctaaaa gatccaggag
     9181 gtacacaata tggggaaacc atgacaggcg gcccatttgt ctggaatgcg acgtataaaa
     9241 atagtgtacg gttgtctact aataccttgc ttctaggaat gatgggagct ggtaaatcga
     9301 ccgttcttaa aatgatcgcg gaagcccatt tagctgcagg agactttatt tggggctttg
     9361 aaaagggaaa agactttatt cctttcttaa aggaatataa cggtattatg gtgcgtttag
     9421 acggatctga cgggatgatc aatcctttag agatatttgc cacacggacg tatgatgaag
     9481 catccagttt atatgacgat ggatcagtca aagatttgaa gatcaatgaa gcagcgtcct
     9541 atcaaacaca cttagacaaa gtggtctatc aggtacagtt agtgagtcct cagctaaaag
     9601 gaacgatgaa agcagaattt aagacctact taaatcgttt ttatgaagag tatggaacag
     9661 taccacgcgg ctttacttcc tcaaattcgc gttcaaatac tgagacgcaa gtcacaggga
     9721 aagatccaga agcttatccg acgttcaaag aatttttaga ctttttagga caactggaat
     9781 taccaggtgc ttcccaagag aaaaagaatc gaaaagaaga aatggaatcc atcgtagaat
     9841 ccttatgtga gacgtatggc atgatttttg atggtcacag tacgattcga caccttgacc
     9901 aacaacagtt ggtctttttt gacattgact ctattacagg cttagatcga ggggtacaac
     9961 aatgtcaaat gtacatggct atgacgttga tttggaatca ggcattgatt caaggaagaa
    10021 gacaacgacg tttaattaaa gaaaaacaaa tggaagaaca agacttcaaa cgcttcttgg
    10081 cgatgattga tgagtgccat aatgcgatta atccagattt tattgaaacg gtaaattatg
    10141 tcacaaactt tcaacgagaa atgcgtaaag tagaagcaat gactgtttta gctacacaat
    10201 ccccacaaga aatggtgcca gaaaatatgt cttctgataa cttctcgaaa ctgaagaaag
    10261 tctttgaatt ttcttctacg aagatcttta tgcgtatgga tgaatctatt ttacgtcata
    10321 tagaaggatt agttggtaaa tcaatgacga actcagaact agaatccatt cctcaacaaa
    10381 atcaaggaga cgctgtgatc aattttggct caaaagaaac cattcgtgtt cattttagtc
    10441 cgaacaaacg tcaattaaaa ttgtttgatg gaggaaaata atggaaaacc aaaatgaaag
    10501 tttaatcaag cagtatgtta agcgtcgtgc aaagcgacgc ttatttttgt ggttgtttgg
    10561 tacttctgca ggtttgatta ctattttgat cactgtgttc gtgacccttt ttctaatact
    10621 ggcggcgggt tcgattgata attctgattc agattcaagc agtggtggcg aagcttttac
    10681 aggagaatat tcggaaggat tacccattta taaagaaatc aaaggaagag gccctttttc
    10741 tgatgagatt gcgcaatatg ctgtaggtgc agcagtgaaa tataaattat taccaagtgt
    10801 gatcctttcg caatacggtt acgaatcagc gtttggtacg tcggcgtccg caagaaatga
    10861 tctcaactat tttgggatta cgtggttcga tggctgtttg tttcctaaag gaactgcacg
    10921 aggaattggt ggtattgaag gtggctggta catgaatttt cccaactcaa aagctgcatt
    10981 ctcctattac ggctttatgg tagccacaca atcaaatttt aatgcatgtg taggaaataa
    11041 aagccctggc gcaagcttat tgatcttagg acgaggtggt tatgccgctg caggaattac
    11101 tgaagatagt ccgtattata caggttgtat gtcaattatc acttctaata aattaaccga
    11161 atatgatgaa ttcgcgatga aacattgggg tgaaggtggt aatgataatg gcacaattac
    11221 tggtgaatgg acgaatccat tccctggcag cagtttagat aaaagtacct ttagcggtgg
    11281 tcaattattt ggaacaaatc caggcagtga attccgtccg aatggcttcc atgatggatt
    11341 agattttggt tcagttgatc atccaggaag tgaaattcac gcggtgcatg gaggaaaagt
    11401 cgtgtacgtt ggcaatcctg gtatttcagg tttaggtgct tgtgtgatcg tgattaatta
    11461 cgacgggttg aatatggtat atcaagaatt tgcgaatagt acaggaaatt ctcgtgtgaa
    11521 ggttggagat caggtgaaag ttggtcaagt aatcgggatc cgagatacag cacacttgca
    11581 tttaggtttc actcgaatgg attggcgtca agcacaagga cacgcattca cagatgatgg
    11641 tacatggatt gatcc
//
LOCUS       DAMYXM010000072        11563 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.075, whole genome shotgun sequence.
ACCESSION   DAMYXM010000072 DAMYXM010000000
VERSION     DAMYXM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.075"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..224)
                     /locus_tag="PNS21_002277"
     CDS             complement(<1..224)
                     /locus_tag="PNS21_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159966.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIV"
     gene            complement(240..992)
                     /locus_tag="PNS21_002278"
     CDS             complement(240..992)
                     /locus_tag="PNS21_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDI5159967.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            complement(1005..2981)
                     /locus_tag="PNS21_002279"
     CDS             complement(1005..2981)
                     /locus_tag="PNS21_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDI5159968.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            complement(3034..3705)
                     /locus_tag="PNS21_002280"
     CDS             complement(3034..3705)
                     /locus_tag="PNS21_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDI5159969.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIKMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAKMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(3787..4953)
                     /locus_tag="PNS21_002281"
     CDS             complement(3787..4953)
                     /locus_tag="PNS21_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HDI5159970.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            complement(4959..5648)
                     /locus_tag="PNS21_002282"
     CDS             complement(4959..5648)
                     /locus_tag="PNS21_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HDI5159971.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            complement(5775..6014)
                     /locus_tag="PNS21_002283"
     CDS             complement(5775..6014)
                     /locus_tag="PNS21_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159972.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            complement(6081..6320)
                     /locus_tag="PNS21_002284"
     CDS             complement(6081..6320)
                     /locus_tag="PNS21_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159973.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            complement(6434..6775)
                     /locus_tag="PNS21_002285"
     CDS             complement(6434..6775)
                     /locus_tag="PNS21_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HDI5159974.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            complement(6762..7091)
                     /locus_tag="PNS21_002286"
     CDS             complement(6762..7091)
                     /locus_tag="PNS21_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159975.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            complement(7682..8014)
                     /locus_tag="PNS21_002287"
     CDS             complement(7682..8014)
                     /locus_tag="PNS21_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDI5159976.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(8851..9891)
                     /locus_tag="PNS21_002288"
     CDS             complement(8851..9891)
                     /locus_tag="PNS21_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDI5159977.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(10240..10566)
                     /locus_tag="PNS21_002289"
     CDS             complement(10240..10566)
                     /locus_tag="PNS21_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5159978.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(10553..11356)
                     /locus_tag="PNS21_002290"
     CDS             complement(10553..11356)
                     /locus_tag="PNS21_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDI5159979.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT         3394 a         2272 c         1688 g         4209 t
ORIGIN      
        1 ttgactattt gatcgtcttc agcaatttta ggttcttctg aagaatcaac tgttgacgaa
       61 tcgacggttt catcgctgct ttgaatcgtg ctgctatctg acgtttggat tgtttgttct
      121 gtgctatccg tgctttcttg tctgtctgca aacgtcaact gtgtgtaggc agtgaaagaa
      181 aaaagagcgg ctgcacaaac aaatagccat tttctatttc tcatgttggt taccctcctt
      241 tattttttct ttttataaac aaatagcgaa ccaatgatca tgactgaaag aaaaacagta
      301 cttcctaaaa gccatttcca agataatttt tgtttcttat tttcttcatc ttttaattct
      361 gattttgtaa aggttactcg cttcccatac actaggaaac ggtgtgtgtt gatcccctta
      421 ggcgtacatg tcattagcgt tactcgatct tcattgggat ttggttctaa gtaagtgaca
      481 aagtttcttt gtagttcgtc tttttgcact tcctcaatcc gatcgatttg ataagcgtgc
      541 tgctcaccat caactttgat ataaaacttt tctccctttt taactgaagg caaatcatcg
      601 aaaagattat ctttataaag tccactgtgc cctgcgatta aaggattttt accgtttcct
      661 cctgtaatat cgccagttcc ttccgtaatc cctacaccat tctctaaaat tttgtcggaa
      721 gttccgtcat aaattggtag ttttaaagaa atacttggaa ttgtaagaat accaacagga
      781 tcgcctaact caatcttagc aacatccaaa gaagaatccg tttctgctgc cgttcctgtt
      841 cgatcgattg cggcaatctg ctgattttgt tggtatttcg ccacgctcgc ttcagcaggc
      901 atcaaaagta agacaccaac agctgaaagt aaccagaaag caatgatcca tttacccatg
      961 attcgctttt tcttagggtg tctgataatc attagctgat ccttctaggc tttcttctta
     1021 aagtaaacaa ctgcagaaat tgcgcctgcc ccaagcaaca agcaagagat caatacgtaa
     1081 gattggaaac cgttgctacc agtcattggt acggaagctt tttgattatt tttgatcgtg
     1141 attcggtttt cttctttgta tgaatctttg tctactttga aagagatctc ttttgttaat
     1201 ttttgatagt tttcaggagc ttttgtttct tccagtgagt aatctccttc agttaaacca
     1261 gtgatttcaa aatgaccctc tttatctgat tttaggattg ctgcatttgc tacatcgtct
     1321 acccaaactg cttctttgac accatcttta tctgtatcag taagtccacc gtagtactta
     1381 ccttctgcat tcttagctgc aaattctgcg ccttcaagcg gtgttgcttt gatacctgtt
     1441 ccaaataggc cgctatcgac ttttaagaat tttgcgccat aagtcacaac gtcagctttc
     1501 gttttttcgt ggtgttcttc ctgattaaat ggattattgt tataaacgaa atccacgcta
     1561 tttgttaatg gatttgcgat ttcagcacta tcattcacaa acatgttata agtcattttt
     1621 agatcttgac catgtaaatt cgcaactgta tcacttactt gtccattatt tacgataaaa
     1681 tcgatcacaa aaccactatc agaattttcg attttatagt cagtattttc ttttaatacg
     1741 actgaaccat cattatttgt tactttaata tctgcttttt cagcaacata agaaagtcct
     1801 ttacctgctg tgtcgcccca tttaaataca ctgtaaggag aagtttcact ttttacacca
     1861 tttactgtaa tcccatctgt tccacctgcg atatctgtag ggatatgaat tgtaccttga
     1921 taattcactg cgtcaccaat ttggaaagaa tgttcttctc ctgttccatt tgtcactgtt
     1981 ttatcgatca ctggtgcttt atagttcacg taggaagctt ttaaatcact tgtcacgccg
     2041 tcattcgtaa tttcaaaggt taacttattg tgcgcatcgt tgcgagcgtc tgcgcctaac
     2101 aaatattggt ctgctgttgg ttcttgatct gatcccacta caacttctga aggaacttcc
     2161 acaaagtagt attttcctaa cgtcaaatca gttgcagtca gctttccttg atcatccgat
     2221 acaagctctc ctaattttgt tccttttcca ggttcgccct tgtataactc aaattttgcc
     2281 ccttttaaag gtgtttgttt gctttgagct gtgccatcgt caccaaattt cgttaattca
     2341 aaactcaact tagaaacaat gttctttgga tacaaatgaa tatcttttaa aaatcctgat
     2401 cctgtattgt ctgtcattgg cgcaatcaca accattggtt ttgctttttg tgtgactaat
     2461 ccagcagcgg atttactttc ataaaccaca tatacattcc ctttggaatt gacataggct
     2521 ggttgatcag caaaagtgat ctcaccattt tcatctacag ctgaagtaat tttattggtt
     2581 gcattcttaa cgtactcact gttagatccc ttgtcttcta catctttaac gatttcgtcc
     2641 actttcgcgt tggttagatc agaatcttca gtcggtaata cttgatccgt aatatttcct
     2701 aacgtaaact caacatcacc ataatctgcc ttgttgtatt tcgttacacc tgtaggatat
     2761 tgatcgtgcg tataaccatc atttttgatt ccgtcaatat caacgttcaa ttgcgtactt
     2821 tgatcgtaca ttaatttatg aaccgtgata ttgacccttt ctggtggcgt tttttgcgtg
     2881 acatcttctg cataagctcg atacccctga aagcagcctg caatgattcc taacattcca
     2941 acaatcattc cagctttttt tacataccga tttattgcca ttttttactc tcctcatttc
     3001 atatttcaac gactcgcgtt gattaaattc ttgttattgt agctctccct gtaccacgta
     3061 tctttctttt ccttgtgacg tataaacaca agtaaacaac gttaataagg gtttctctcc
     3121 tttctttggt aaagacaaga aagagttctc ttcaggaacg ccatcgatta atttaaagta
     3181 ctcaggtacg ataaatttac gcgttacctt gtaacgatag acgccatcgt tactaactaa
     3241 gtccatcacc tcccctaatg acaacttttg aatatcagaa aataaaacac ctggcatttc
     3301 catgttatga cctgctagga catagtttcc tttgcccatt ttggcatctt cataaaaata
     3361 agtagccgct ccaagactca aagtgtattg atttgcccct ttaaaaattg gcaatgctaa
     3421 accaagtgca ggaatctgaa tttgtgcaaa tacttgttta ttgacgccca ttttgattgc
     3481 ttctttttgt atggacaaca tttgccccaa agacatatca ttattgacgt tcgttgcatc
     3541 ctccttataa ctctttgcgt gtgcattgat ttgatctacg ggcatctttt tttctatatt
     3601 ttgcttaaac acctctcctt catgtttttg gtagctaaca tagccaaaca cactgcaaac
     3661 gacgattaaa gcgactccta aaaaaccaaa taaccatttt ttcatccatc tgcctccttt
     3721 cagacaaaac aaaaaacact ttttcacaac gaaaaagtgt tttctcttat tactattcta
     3781 tttcatttat ctggtggttt tatattggtg tgtagccatt gctaacatta agaatacaat
     3841 tggcacccaa ctgattgtcg ttaatgtttc ttccatgaac gattgacaca tgatcgctaa
     3901 aaaagcccac aagaatgtta gttgtccatt tgtcagctca cgttttctta cgagcaacca
     3961 taagaaacta agcaaaagaa taatcgatag aacaaaacca atcaatccat agtgattgac
     4021 gagtgcgtga tacgtattat gattacctag aatctgattt cctctatgga aaaagaaagg
     4081 tttcatccct acaaatattt gtcctgtttt ttcgcctaat gtttcataga atttgcgcca
     4141 aatttcttct cgtcctgtaa ataagtgaac tttgtctgac attgcagcaa aataagaaat
     4201 cggtaaagct agaaccgaaa ctgctgcata catccaaaag gacaagtgtg tatttcgaaa
     4261 aaccttctta ggtaaaattt ctaagataat gaagataaca agcgcatata atgctgcttt
     4321 cgattggcaa acccaaatcg ttgccccagc aagaatcaac gctggtaatg atagcaatcg
     4381 catgtaccat tttccaaatg aactagcaaa tcctgtgata agaagggcta atgtcattaa
     4441 agaactacct atcgtatttg tattgatcca aatatctgat aatttgttcg atggattcca
     4501 aatactttgt ccttctggta ctggtatctc agtaaatact cgataaaccg ttacgagtaa
     4561 aaagacatag aatactacaa gatacaagct acgatcaatt acagtaaatt tcgtccattt
     4621 tacaatcaaa ataatagcaa taattgtcgg aacgatcaga tcaccataga aagtatctcc
     4681 tcggtagctt ctaaaaaaag aaaagaaaag gtaaaaaaga atgcctgctg caatgaagaa
     4741 tagatcgttt ctctttacct tgtacagatt caaaagaaac aaacagccta cgaaaaacgt
     4801 cactaactgt gcagcattat cataaataaa taatatcgta ggaggattta accccaccgt
     4861 ccatattaaa tatagaatgg ataccaacag taacagaaaa atggcactca ttttattgat
     4921 caatttttct acaagttctt gaatgtgact cacttccctt atttattgat tttttctaca
     4981 ttgactaatt cacactggat cacatcgcac tttttcatat tatttgcttg aaaggctaac
     5041 agtgtgagta gtggtttttt acctgctttt ggcaaggata aaaaggaatt gttttcctta
     5101 gattgaacta cctggctgta atcagaaatt tttcctcttt tggagacttc atactgatac
     5161 ttgtaatgag catcgattag atcaatttct tctccttcag ataaatcagt catatcggat
     5221 aataaaacgc ttggtgtatc tacgttataa ccagcaagaa tataatttcc atttcccatt
     5281 tcagtatcta gataataagt agaagctact cctaaactaa gcgtgtcgct attcgctcct
     5341 tgaaaaacag ggagaataaa tcctgtagaa gaaaaaacaa gttttccagc aatttcacct
     5401 ttgagatcct tttctaatgc ctgtttttga ttagtaatca gttgtctctt agaaatatcc
     5461 gttaaactgg ttaaattatc ttgataggtt ttagcctgtt cctgaatatg gattgttttt
     5521 gttacaccat ctactttttc agcgattttg tccttttgat aaccttgata agcaaaataa
     5581 ctaaaaagaa gaaaaacaat gattacgccc acgtaaggat taaataaagt tttattgttt
     5641 ttattcatct atctaccttc tatatatgaa ttaatttacc tacatctaac caagataaac
     5701 catgttaaat tttactattc tttttattca ataacaatgc cacatcataa ttgatcattt
     5761 tcatatcata atcgtcaaga aacttctaat aatagttggc aattggaacc tgtttttaca
     5821 taagttcgcc tcaactgttc aaagtttttc attgaaccta acaaacaagc tttctctttt
     5881 cctgttatct tcatatcatt gatatattgc atgacagata atcttttaag gtgtttttgt
     5941 ttcatcattt ttagcaactt ttcttccatt tctttcgaca ctttttttct gttttcgatt
     6001 tcttcataat tcatgagttt tcctaccttt cttgtttttt gcaaaaataa aaaagcctat
     6061 gatcacatac gctctacact tcagtcttgg gttatctttt tctttcgttt aattactggc
     6121 cgtgctggtt tcaaatcctt gattccatta tgggttctcc aataatcgaa ttcttttcgt
     6181 aatctttctt tttccatatc tgttccttta gacagggatt tgaatacatc tttaggtgaa
     6241 taaccacgag ctaacagctg tacatacctt ccttttgtca aagtatccat atccatcgcc
     6301 tctaaaactg gtcgcgccat ctaattttcc tcctaaataa attccttact caatgcctaa
     6361 ggtaatacta cctttttatt attcgtcgtg actccttcta agcctttatc tgtaaaattt
     6421 attcctccag tttttattct tctttatccg ctacaaattc aatgatttca acgctgacaa
     6481 tataatcact caaatatgac caagtatctt caacagataa ttcgtctcca ttatcatccg
     6541 taagcgtcat tttaaaccat tcattacctt catcatcttc aggtagcaag ccgaatccaa
     6601 ttggattatc agtcaatttt tgatcgtata aatcatcctc gtctaatgct aaaacatcta
     6661 atcctttaca atttccgcct cttctaaacg tcacaaatcc tttgtaatcc caacgttgga
     6721 aagaaacctt aatcgtatgt gtgcccaatc gtatattcgg atcataattt ttcatttgtt
     6781 gtaacctcct cataatcagt tagtactata aaggtatctt tgtttttatc caaaaaatct
     6841 aataaatctt ttgtagaaga tatctcttca tcaacttcac cataataaat accttcgata
     6901 attgttgcta tatctgaaaa actaattttt actggacatt tatcgtctac cgcattcaat
     6961 accgacaaaa ttgcttcttt ttctgcaatc tctcgtttga atttctttgg agctaatcgc
     7021 atgacactca agtcattttc aagcttttca atccatgcag ttttactcat tttcgcaaat
     7081 ttttgaatca ttcctctacc acctttgcta tctactaaat tagttttact tcataacgaa
     7141 tatctttatc gtttttcgtt cactcaaaaa tatataaaat aaatcgactc gtttatggcg
     7201 tccgaaacga gaaaccctag tagacagcgg taaatgctcg attgatccaa tgatcaacgt
     7261 cctcgcaagt atctacatgt actgcagcac acgacacaat ttcttgtgcg tcctccccat
     7321 tacgagaaga cctactcctt attccatgag tttgcgtcaa agcctagatc ccttagtagc
     7381 tgctcatgat taatctaggc gctattctaa cttgtctaac ttctaaattc cctcttgttt
     7441 actctgtgca tattatttac acaaattcat ttcaccacta tccaaacaag ggcatttact
     7501 cgattgacca attttttacg ttaccaatcg taaaaaccta ccccgtcact ccccgaaggg
     7561 aattgttgat agatcaagtc tactccttat ttttctagtg attctaagtt cctttagaag
     7621 tctcgacaat attttcaagc aagcgaaaaa cgataaaata caagttatga gaatgatcaa
     7681 ttcagctgat taaacgcccc atcccttttg cggtacaacg caagactcca ttttttcttc
     7741 gaccaatcag tacaccgttt tggtcataaa catgtttgat tgttggatat agctctttta
     7801 aagggatagg atcttttaaa attatttgtt tttgtttccc aatgtaataa atcatttctt
     7861 ctgagacttt gtctttctgg attatatatt tcttacactg cacaacggtt ttactttgac
     7921 cagttgatag tttcttcgtg atcttttctg tagcggtaca ccgtttaccg tagtgacaaa
     7981 tatcttcgag actcaaaatt cctgtatatg acataaaata atccacccac ttactttaaa
     8041 tatttttaac aagtttttct tgttattttt caaaaataga gaattgattt tcgttcatga
     8101 caaacaaaaa agagggaacg cattcaaaca tgcgttccct ctaatttcag cgttcgtcat
     8161 acggcaaaga gccccacgga catttttata acattatgct taggcataga ttcatcgtaa
     8221 tatcacgtat ttttaacctt aacacagcca aaaaaagtgg tatcactttt gaatatctgt
     8281 tatactaaat ccgttacggg taataccgta acactgaaag gtaaacgcat taaggggtat
     8341 agacttaatg ccacctgtgg acgtcgacga aggttaccag ccttccgagt ctcacagggc
     8401 ctttcttttt tttgtatttt caattgtcca aatttatcgt ccaaaagtgc agaacgaaaa
     8461 tcaattcatc acaagcatag cagacaatta aatttaaaac aactaaaaat atacaaaaaa
     8521 gtgtaattga atattagcag cactgcctga aaagaagtaa tatcaggctt ttcagcttgg
     8581 ggaaataagt gaattacctc tcttcatgaa ttattataac tcaaaaaagc acatatgcaa
     8641 acactagttc cctatttttt tgagtgaaaa ataactaaaa tatctaacga ttatgaattt
     8701 aaaacctacg attgtgattg ttttcataaa cttttaatct aaaaactccg gaatactaac
     8761 tatttatttg taatatttgt aataaaatta aaatcataaa aaagttctaa tttaaaattg
     8821 atcattttta tagtaattgc tttttatcaa ctactctaac caattttccg tactaactgt
     8881 tggtaattcg ttaaatgttt ctctttgttc gtttgcaagc tttgccgcaa tagctttttc
     8941 gacaaaccaa ttttttacat aaacgaaaat tagattctgc aaattgttta ctttttcagt
     9001 tttttgtttt tgataagctt tcagtaaagt catatataat cctttgtcag catcaattcc
     9061 ataagattcc agttcttcat aaacgacaat agtttgctct aattcttcag cttttttctt
     9121 tgcattatga atagtttgga cggtttgttg agcttcactg taacttgaag aaaatgtttt
     9181 tatcaagttt aatacatttt ctggtacaaa tgtatttata ctgtcctctt gcatgatgtc
     9241 gacgaagtta tctagcaaaa cttgattttg taactggtca gtttcagtgt ctattatgtg
     9301 tctattagtg tctatagaat tagaatagaa tagattgtct gcgattttcg caattacggt
     9361 tgagtccttg atatgactgg attttgccgt atttgcgatt ttcgcaatta cggtagaatc
     9421 attgattttt cgcggttttg ccgtatttgc gattttcgca attacggtgg aatcagtatt
     9481 gtcttcctct tccatgattt taaagacgtc ttgattcgta acttctggct tgcctaagta
     9541 caataaatta ggcatagatt caatcttacc atcacgttta tttactcttc ctctcttctg
     9601 tttcagaaga cctgcttttt ctaattcttt tttgatctta gatactttac cttcgcggca
     9661 ttgtaacaat ttcattaatt cttcaactgt gaatatgaag aatatattat catcagaatc
     9721 tacccagtta tttttgactg aataatcaaa tcgatcttta agtagcatat aagcaatttt
     9781 tgcatcattt gaaagatcca tgtatttttc gttagtaaaa aataccttag gcatttgata
     9841 atatttttca cgataaattt cttttgattt gtaataatta aaatcattca tagccaatcc
     9901 tctttctgta ggatagcgtt ttgttcaaaa ctatgttaca atgaattaaa caaatatatc
     9961 tatccactat atatttttct actgctgata agtcgccaaa ctttgttaaa gcagtagtct
    10021 ttttttatct ttttttagtt taccttaaaa aataaattaa agcaaaccgc tctatttcag
    10081 ggtttaagct catggtaact atatcacgat attacaataa cattatatca cgatatcgta
    10141 atattacaat atgaagataa gcgacttatc agctttgata ctactgattt ttataaaaaa
    10201 aatatcacga tattatgata tcgtgatatt gaatgtaaat taaattttat tttgtttctt
    10261 tgctagttta tatccttcaa tcaagtattc ttcaggtatg gattttaata gcgcatcttc
    10321 tataacgtct ggtttacttt tgtctgattc agcaatagtc gtccaatatt tgagtgctga
    10381 cattagaatt ggatttatac gaatgctatc tgctgttctt acactacttc cttgatcttt
    10441 tatgacttta cttatatttt tcttggttaa ttcgatttct tctacttctt ttggtttgta
    10501 gttcttttta tttttatttc ctagtagtct acttactgca gcttgactgc gattatcttc
    10561 tgccatcaaa atcaccaact tcttttaaat attcttctgt aaatgcttta tagtcatctt
    10621 cagctgttga gggtttatta aaattgatta gatctgatcc agcagcttgc gcttcgtcaa
    10681 cggttattgt tctacgaata taagttttga aaatttttgc accaatgact ctagaaattt
    10741 cggttgctgc agtcgtcatt tctttagaaa tgttataacg cttattatac ttagtaaata
    10801 ggactcctcg tacttcaagc ttgtcgttcg tatattcttt taccgtttca atagtttcac
    10861 ttaactttat tagtcctttt aatgagaaat acgcagcttc aactggaatg ataatttcat
    10921 cagaaacagt aaatgcgttg actgtaagca ttcctaaact tggtggacaa tcaattataa
    10981 tataatcata gttgtccttg attggttgaa gtactctttt aagcctgtgt tcacttccta
    11041 cttgagtaaa ttccctctct gcagaagaaa gggctatgtc ggctggcaaa acatcaattc
    11101 ctttatagtc cagaatagca tctgtaatgt cagcctcccc ttttaatact tcataaattg
    11161 taggttggtt tgttccatct atatcgaaac ctacattatc tgtagcattt ccctgaggat
    11221 caatgtccac taaaagtaca tttttaccgt aaacaaattt caaacatgct gctaaattaa
    11281 cgctagtcgt agttttagaa actccacctt tttgattggc tactgacaat attcttgcca
    11341 ttaaaatatc ccccattcat cttatattat aatataatta tatcataata ttgtgatatt
    11401 gcaatatcgt atatgcatta tattgtaata ttgtgtgatt attttgtttt aaagatatat
    11461 atcggttcgg atataaagat tattcatatg ttattcgaaa gtaaaataat cactttttat
    11521 tactttaaaa ttttttatta atcataagaa atatgttttg cga
//
LOCUS       DAMYXM010000073        11514 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.076, whole genome shotgun sequence.
ACCESSION   DAMYXM010000073 DAMYXM010000000
VERSION     DAMYXM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.076"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(59..2134)
                     /locus_tag="PNS21_002291"
     CDS             complement(59..2134)
                     /locus_tag="PNS21_002291"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDI5159980.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2136..3053)
                     /gene="glyQ"
                     /locus_tag="PNS21_002292"
     CDS             complement(2136..3053)
                     /gene="glyQ"
                     /locus_tag="PNS21_002292"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDI5159981.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3439..4257)
                     /gene="recO"
                     /locus_tag="PNS21_002293"
     CDS             complement(3439..4257)
                     /gene="recO"
                     /locus_tag="PNS21_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDI5159982.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(4400..5299)
                     /gene="era"
                     /locus_tag="PNS21_002294"
     CDS             complement(4400..5299)
                     /gene="era"
                     /locus_tag="PNS21_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDI5159983.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5314..5709)
                     /locus_tag="PNS21_002295"
     CDS             complement(5314..5709)
                     /locus_tag="PNS21_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HDI5159984.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(5693..6169)
                     /gene="ybeY"
                     /locus_tag="PNS21_002296"
     CDS             complement(5693..6169)
                     /gene="ybeY"
                     /locus_tag="PNS21_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDI5159985.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6198..8390)
                     /locus_tag="PNS21_002297"
     CDS             complement(6198..8390)
                     /locus_tag="PNS21_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDI5159986.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNTGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8414..9385)
                     /locus_tag="PNS21_002298"
     CDS             complement(8414..9385)
                     /locus_tag="PNS21_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDI5159987.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10090..11469
                     /gene="brnQ"
                     /locus_tag="PNS21_002299"
     CDS             10090..11469
                     /gene="brnQ"
                     /locus_tag="PNS21_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDI5159988.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3102 a         2440 c         2126 g         3846 t
ORIGIN      
        1 caaaaaaaac agagacacag cacgtttcgt gcatgctctc tgtttttcaa acaaaagact
       61 attttgtgat gatttgatcc aagcttgcta atgacaaagc catggcagct atttgactta
      121 gttgtgttag tcggttctta cgtactgctg gttcatcagc catgaccatc gtttgatcaa
      181 aataattttc gatcagtgga cgaagagcga caagctggtc gtagttttcg tggatcgttt
      241 ggtcagcaaa acttgctttc attgcttcta ctgctttgtg taattctttc tctgcgtcgt
      301 tttcgaacag ctcagggtct acttctgatt gtttttcaag ctgcgctttt ttcgctaagt
      361 tcacgacacg tgtcaaagct tccattgaag gtttgaagtc ttcatctgca agatgttgtt
      421 tgaacaattg tgctgctgca aatacttttg tcagatcttt ttgttccgca ttcaaaacag
      481 catcgatgac atcatgacga atattcttcg ttgtcaataa ttggcgcaga cggcctttga
      541 taaagtcgat cacttgttgt tgcccttctg ataaaccaat gccgaaacga tccgtatctt
      601 tactgataac atcgctaatc gtttcttgca gaacagacaa aggaaaggcc cattttttcg
      661 attcgatgat ccgaacgaca ccgtatgctt ggcgacgtaa ggcataagga tcgtttgacc
      721 cgcttggaat catcccaact gcaaagaaag aaaagatgct atctaattta tctgcaatcg
      781 ctaaaacaga acctactgta gactgaggta gctctccttc actggatata ggcatgtaat
      841 gctcacgaat cgctgtcgct acagcaggtt tctcgccttt cagaagagca tatttttcgc
      901 ccatgatccc ttgtagttca ggaaattccc ctaccatgtt ggttactaga tcaaatttat
      961 agatttcagc agctcgttgc aaatccatca attcttcctc agatagtcct acttgttcgc
     1021 caatgatacg ggcaatgact tgtacccgtt gcattttctc atagatacta ccgatttttt
     1081 catggaaagt aacagttttt agtttttcaa cgtattggtc gattgaagct ttcttgtctt
     1141 cattataaaa gaattccgca tcttctaaac gagccgtcaa tactttttga ttgcctcgaa
     1201 ccacatcttc caattttacc cgatcaccat tacgagcaga aataaaatga ggaagcagca
     1261 taccttgatc attccgtacc tcgaaataac gttgatgttc tttcatagaa gtgactagta
     1321 cttcttcagg aacagaaaga tatttttctt cgaagtcgcc agaaaagaca gttggatatt
     1381 cgactaaatt gttgacttct tctagtaaat cttcgtctag atcaataacc cagtgatttt
     1441 tttctgctaa ttcgtttgct tgtgtaacga tcatttcttg tcttttttgc ggagacgcaa
     1501 tgacgaattg ttcattcaat ttttcttcat attcttttgc atgcgcaaat gtcacatctt
     1561 cgcctaagaa acgatgacct cgagaggctc tgcctgtttt gacatccaag atttcgaatg
     1621 ggattacttc gtcatctaac aatgccacga tccagtgaat agggcggata tattcaaaat
     1681 cgtattctgc ccaatgcatc gttacaggga aagtcaaact tttgatcacc cctgctaagc
     1741 ctgtcaatac ttcttttgct tccttaccat gagtgtattt cgttacatag acatattcca
     1801 caccttttaa ttctctaaaa gtgatagcgt ctgtcgtcag accttgtccg cggacaaatc
     1861 cttccgctgc tttcgtccag tttccatctg cgtccaaagc aatttttttg gctggtcctt
     1921 tgacttcttc ttcactgtcc gcttgtcgtt ctggaatatt tgttactttg attgctaaac
     1981 ggcgcggtgt ggaaaatgtt tcgatcgtat cataagtcaa ttggttttca tctaaaaatt
     2041 ttgctgtttt ttcttctaat tgtttcatgc ttggtgtgac aacatgggca ggcatttctt
     2101 ccagcccgat ttcaagtaat aagtcttttg ccatcttatt cagcctcctt cacggtttct
     2161 ggatttgctg tgtttttatt caacaaaggg aatcctaatt tttcgcgttc tgccacaaag
     2221 attttggcta cagcacgagc catgttgcgg atgcgagaaa gataaccggc acgctctgtt
     2281 acggatactg caccgcgggc gtctaataag ttgaatgtat ggctacattt caagatataa
     2341 tcatacgccg gatgaacaag attttcatcg atacagcgtt tagcttcttt ttcaaaacga
     2401 tcaaagttgc ttagtagcaa gtcttggtcg cttacttcaa aagaatattt ggaatgttca
     2461 tattctggtt gtttgaagat ctcgccatat ttcacgccat cagcccattc taaatcatag
     2521 acactttcaa cttcttggat ataagaagca agacgttcta atccataagt gatctcagaa
     2581 gttactggtt tacaaggcag tccaccaact tgctggaagt acgtgaattg tgtgatttcc
     2641 attccgtcaa gccatacttc ccaacccaca ccggcacagc ccatggatgg gttctcccag
     2701 ttgtcttcaa caaaacggat gtcgtgttct aatggatcga ttcctaaaag gcgcaagctt
     2761 tctaaataaa gctcttgaat attctcaggt gaaggtttca tcaccacttg gaattgatga
     2821 tgctgataca aacggtttgg gttttcacca taacgtccat cagctggtcg gcgagatggc
     2881 tctacgtaag cagcattcca tggttctggt ccgattgccc gtaaaaaggt atatgggctc
     2941 atcgtacctg ctcctttttc tgtgtcataa gcttgcatca gcatgcagcc gtttgatgac
     3001 caaaattttt gtaatgttaa aatcatatct tggacggtca attttttact catctgtttt
     3061 gcccctttca attttaaagc gttgctgtca aataagaata caggaaagaa aacgacaaaa
     3121 aatccctatg tctgattaca gacataggga cgaataattc gcggttccac cctacttccg
     3181 aataaagatt cggcagcttc atttatcgta ctcgcaagtg cctcttcaaa aatcgtttgg
     3241 gtccggctcc cactctcccg aactcgctaa accacgtgtt ttttactgat cttggtcaat
     3301 gtacgtattc attttgcagt tttcttgttt ctataataga tcctatcact gaacgatcta
     3361 tttaatctat ggttaataat agacggaact ccctgtgttt gtcaaatggt tttgattttt
     3421 tgttgtatgt tttatttact attcttcttt ttcaggtgtc tctttttctt tttcagacat
     3481 gtctttctct gacgtcttta atacgtgtgc ccaagacttc atttgattga tgaacttttt
     3541 gcttttcaaa tggataccga catattcatc atacagctgg tcgatgagtt cacggattgc
     3601 ttgtttggtt tcttctttca gatggattcc actaatttta tcataagaaa tagctgaaaa
     3661 catccgaacg aaataaacag ctcgaggatc tgcatgataa cggtgatcgt ccatcgacca
     3721 atgtctttcg catatcacac caccatattt ggaagaatag tcaaattttc cttttgtttc
     3781 cccgcatacg acgcagtgtg tccagattgg tgcaattccg aagcgttgca gtatttggac
     3841 ttcaaaaata ttcgtgatga tttctgcatc gtctccttgg tccatccgtt gcagtgcttg
     3901 ttgcgtaaac tggaacaaat gcgggtcgta ctgatgatct tcgattgccg catctactag
     3961 attcaataaa tatgtgccat agccgctcaa aaagatgtct tcttggatgt gtcgaaacgc
     4021 atggacatct ttgacactgc ttaaaaatga caaaccttca cttcgaaaat tcccgatata
     4081 aactgcttca gtaaaaggga gcaaagccgg agctaagggc tgattttgcc gatggatctc
     4141 tttgatataa aacatcattt tgccagaaga ttctgtaaag attttgacta atttatcttt
     4201 ttctttgtaa ttacggctag ataaaatcaa gccttttgat tctgcaagct ggctcatatt
     4261 ctccctccta aaaaataaat ctggaaaagc agattctgcc gatccagatc atttatgttg
     4321 cgatcttccg cagcttttct caacacgcgg atttttattc agtttatttc tatacctaat
     4381 ttacaagaag ctcagaatat cagtattcgt cttttcggta gccgaagtct tgcagataga
     4441 cttttttgtc tcgccagtct ttttgaactt tgacccaaag ttctagaaag actttgtctc
     4501 ctaaaagatt ttcgatatct tttcttgctt tcgttcctac ttgcttcagc atttttccgc
     4561 ctttgccgat aatgatccct ttttggctat ctctttcgac aataatcgta gcctggatat
     4621 ggattttatc attctcgttt cgcttcatcg aatcaacaac gactgcgaca gaatgaggca
     4681 cttcgtctcg tgttaaaaac aagactttct cacggatcaa ttcagaaacg atgaagtatt
     4741 caggatgatc agtcacttga tcatctggaa aatattgtgg gccctctggc atttcactga
     4801 ctaatacatc catcaaacgt tcgacgttgt ttccttctgt agcagagatt ggaatcactt
     4861 gtgcaaaagg catctgcgtt gaataatctt cgatgattcc caacaactcg tctggatgga
     4921 ttttgtcgat tttattgata atcagataaa caggagaatg cacgtttttt agacgttcaa
     4981 taataaaatc gtctccccga ccgcgttttt gatcagcact aatcataaat aagaccgcgt
     5041 ccacttcttt caacgcactg taagctgctt ctaccataaa atctcctaaa cgatgctttg
     5101 gtttatggat tcctggtgta tcaataaaaa tcaactgagc ttctggtgta gtgtatactc
     5161 cttggatttt atttctagtt gtctgtgcct tgtcactcat gatggcaatt ttttgaccca
     5221 cgattcgatt caataatgtg gatttcccta catttgggcg gccgacgatt gcgacaaacc
     5281 ctgatttatg ttctggcata tatgttctcc tttttaatta aacaacaaat gatatatttt
     5341 agggacaaat aaaatcaacc caacgatgac ggtaaaacaa gcggtcacta aaactgctcc
     5401 agctgccata tctttgattt ttttcccaat cggatgaaaa tgaaaatccg taaacatgtc
     5461 cacgacattt tcaaaaacag tattgatgat ctctactatc catacgagaa aaacagctaa
     5521 aagcagccat aaccattcta tccgatcaag ttggaaaatc acgcccataa acaaagcgag
     5581 gattccgaac gcaacatgtt tcctcatgtt gcgttcttcc tcaaaaacag ttttgactcc
     5641 ttggacggca aattccacgg acatcaggaa atgtttgttt ttctctacgt ttttatcttt
     5701 taagtccata ggcatctaag atctctttct gtaaaccaaa catttctttc tcatcctctg
     5761 gtgtcatatg gtcatacccg ttgatatgca agaatccatg aaccgctaaa aagccaagtt
     5821 cccgatcata cgaatggccg taagcttctg cttgctctgc agcacgttca gtagagatca
     5881 tgatgtctcc cagtacacga ggcaatggat tctctccatc ttcaaaaatc actgggattt
     5941 cgtcttcgcc ctcttcttcc aaggcaaaac tgatgacgtc tgtcggtgca tcttttccac
     6001 ggtaatcacg attgattact tggattgctg cattgtccat gaaagtaaca gacatttctg
     6061 tttcttctgg caactctaaa tatgatgctg caaattgcag caagtcgtcg atttctttca
     6121 ttttctcttc tgagacttgg tttgtttcat ccatgaaggt aatatccatt atacatcgct
     6181 tctttctatt cttcttgcta ttcttcttgt tcttctttta tcttctcaac ttccgatttc
     6241 attttaggat atttgatccg agaatggaag gtactgctta aaccgctgat caatgatttt
     6301 tctactttac ggatttcttt taacgtcaat ccactgtcgt ccaattgtcc atcggaaata
     6361 cgctcttcaa taagattatg gacaaaactt tcgatttttt cgctcgttgg atggtccatt
     6421 gcccgtactg ccgcttcaca actgtcagct atattgacga ttcctgcttc acggctttga
     6481 ggttttggtc caggataacg gaattgctct tccgtaactt ccgggttgcg ttcctttgcc
     6541 ttaacgtaga aataacgcat cagcgtggtt ccatgatgct gtctacaaat atcgatcacc
     6601 atttgcggca tattataatc ttccaatatt ttggcaccat ctgtcacatg tccaaagata
     6661 atatgtttac tgtcttctgg caataagaaa ttgtgcggat tttccgctcc tgaaggcaag
     6721 ttctcaacga aaaagctggc gtgtttgatc ttgccgatat catgataata gcaagctact
     6781 cgagttagta aagaacgtcc accgatttct gctacagcat tagcacttag attggctacc
     6841 atcatgctat ggtgatacgt acctggtgct tcttccagca actgtttcaa taaaggatga
     6901 ttgggattgc tgagttcatt taatacgatc acactatcat cattgaccat caattcaata
     6961 taggggtgca gacctatcgt cagcaagaat gaaaacagtg cacctgtgaa tccgcagact
     7021 agcgttaacc aagtcgttgc atcaccaaag ctcattcctt gatagacgat caaaatgaca
     7081 tccatcatca caggaaaaat aatgacccac atggcagcag ataatccttg ttctgagatc
     7141 cgtttacgtc tgacaatcgt tgccatgagc ccagagaaaa cataagacat caagataatc
     7201 gttagaaagt tcgtgccaat cgaatcataa aaaatgaaaa gtgcggatac tgcttggaaa
     7261 ataccagcca tgatccctgc tcttcgattc aagaaaaagc ttaaaacaag tggtgcaaaa
     7321 gcagcgggat aaaacagcgg gatataagta gcttgttccg tttggaataa ttggaaaaat
     7381 ttcatcagca ctatgctgac tgacatagct gtcacataga aaagaacgta ttttgttctt
     7441 tctcctgtat tttggaattg aagtgaatta taaatcagta cgccgatttg aagcaaaaca
     7501 gctaaaacca tggcaattag cggaaaaatc gaagttgttt gattagtcag tcctagtaat
     7561 tccaattttt tcatagctga agcatcaatt tgattacctt cacgaacaat gatttctcct
     7621 tggaagatca tcactggttg tacagcttct cgtgcggatt gtttcaattc ttctgtcttt
     7681 ttctcattca atacgtcatt cactacgata ccttgatcca ctaagtagcg aatcatttgt
     7741 tgttgtgctg gtgtgacatt caaatattgg atttgctctt ctgcttcttg tttaaaagct
     7801 tccaattcac tttcacgaac ttgtttgctc atctgttcgt caatgagctt cagactctca
     7861 tccctcacag tatgaatctc agtttcagtc atttcaaata tcgtcgaata aaaattgttc
     7921 ggtagttttt gataaaacac aacattttct gcattcacat tttcaaaatt tttcttcagc
     7981 gcggcgatcc gctcttctgt agtaggcttt gggatatttt catcttcctt tgccttatca
     8041 atcttttcgt cataagattt gttgatggca tcattcgtct tatcgatcaa ttcaaatagt
     8101 tgcttgatcc gattgttctg gtcatctgca agatcttttt ggtaagtgta ttctggagtg
     8161 actgcttctg ctgccagttt tcttttttgt tcggtttctt ccgtgttctc gatcgttttg
     8221 ttcgcacgga tactttcttc tgctacttgg ccttctctga aatcattggc cttttgtttg
     8281 acactactaa atgtaacgcc gcagataatg ataaaaaaca agaacagcag gaaaggcaat
     8341 gctgcctttc caagtttttc ttgtagtttc tgcagtgttt tattcaacat gtgcttatcc
     8401 tcctaagcaa agcttattct tgatggagat ctgccttttc ataagctttg atgatttcgg
     8461 caactacagg gtgccgaaca acgtcacttg cttcaaaatt aacaaaatcg attttcttga
     8521 tttgctttaa tgttcgttct gcatgaacaa gaccacttgt tgtccctcga ggcagatcaa
     8581 tctgactcgt atctccattg acaatcattt ttgattgata accaagacgt gtcaggaaca
     8641 ttttcatctg agcaactgtc gtgttctgtg cttcatccaa aataacgaat gcatcatcca
     8701 atgtccgccc ccgcatgtaa gctaaaggag caatttcgat cacgcctcgt tccatcaatc
     8761 ggttcgtatg atctaatcca aaaatttggt ataaagcatc ataaacagga cgcagataag
     8821 gatctacttt ttcttttaga tccccgggaa gaaagcctag attttcccct gcttccacag
     8881 caggccgcgt caaaatgatt ttttgcactt cacctttttt caaggcagcg attgctaata
     8941 ctactgcaag aaaagttttt cctgtcccgg ctggtcctac gccaaatacg atatcatgtt
     9001 tggctaccga ttcgacatat ttcttttggc cgctgttttt tacccgtata ggttttccat
     9061 tacggtcttt gacgatctct tcctcataca tctcaacaaa ataatctaat gtgccttttt
     9121 gccccatttt cagtgccgtc acgacatctg gtgtacttac atggattcct cgtttgatca
     9181 attcctgcaa tgtccgaatg actgaggcag ccaaggaaat ctgggattgt tcgccaatga
     9241 tctgaatgat ttctccacgt gtatggatgg ttgtttctgt tgtttcttca atcagtttta
     9301 tgtgcttgtc gtgtgcacct aaaagcatgc tgacatcatc agcagccgtc aatctaatct
     9361 ctaaagaact agactcttcc gtcaaaaata gacatcccct ttttttcgat tcattctttc
     9421 ctattttacc atactcagaa gaaactatat agcaaataag aaaaggttct aaaagagttg
     9481 cttaaccata agtaccaaga aacgttaaaa aatagctgct tccattctct aaaggtctcg
     9541 tttgaataaa aaaagctgat catgtaagct atgcataaga tagaggacaa gtattccgtc
     9601 taacttagca agtattctct gggtattttt cttgtttcaa aaaaagaaaa caaaatattt
     9661 gaaatattct tgcatttcaa aaaaaacagg tgtattataa cgtcaacaaa taaatataca
     9721 gcaaagcgtc gaggaaaaca gtaggctatt cagtgattta ttcccagtga gtcatgtaag
     9781 tgagaaatga caataaatcg aatagacgaa catcatttcc gagtcaatgt cgaaaacgta
     9841 gatcattgtg gctgcctcca ttatcaggcc agcggataac tttatcgaaa agcgtccgtg
     9901 aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta gagccctttt
     9961 cccaagtgga aaagggcttt ttttatattt taaggtggtg gttttcatgg aatctgatat
    10021 tagtggtatt tctagtttct gcaacgttta ttttgttact caaaatgata attattggag
    10081 gaaaaattta tggaagaaac aaaaacttat tcatggaagc aattattgat ggtcagttca
    10141 cttatcttcg ggatgttctt cgggtcaggt aatctgatct ttcctgtcca cttaggtcaa
    10201 atggctggat caaactggat cagcgctggt gtaggatttg ccatctcagg cgctcttttc
    10261 ccattattag ctatcttagc tgtagtagtg acaaaaagtg atggtctgta tgacttagct
    10321 aaacctgtcg gaaaatggta tgctgccttg ttcttgatcc ttgttcattt aacgatcgga
    10381 ccattctttg gtacaccgcg gacagcagca actgcttttg aaatggctgc taaacccttt
    10441 ttgcctgaaa aatatgattc attaggtatg tttttattct ctgctctttt ctttttactt
    10501 gcttatttcc ttactgtaca tccaacacgt ttggtcaaat atgtcggaaa gtatttaaac
    10561 tccttgtttc ttgcattatt agctatcatt tttgtcgttt ctttattcca acccatgggg
    10621 aatctaaacc aatctgctgc agattcttat cagacgaacg ctttgacaaa tggcgtttta
    10681 gaaggataca acacggttga tgcagtagct ttacttgctt taagtgtcac ttttgtccat
    10741 gcagttcgtg atcttggttt taaagataaa aaagtcacag aattaacagc aaaagctggt
    10801 agcctgagcg ttgcgttaga agtcatgatt tattttggtc tggtccttct cggtgcgatg
    10861 agtttgaata agatgtcttt atcagaaaat ggcggaactg cactttctca gatcactaat
    10921 ttttatttag gtaacttcgg tgctagcttc ttaggggtaa tggtcacatt aggtgtcttc
    10981 actaccgcta tgggactcgt tgtttctttt gcacaagatt tccataaact gttccctaaa
    11041 gtcagttata tgacatggct gcggttaacg acttttgtct cttttgtcgt tgcgaatgcc
    11101 ggattggata atatcatcca atggtcttta ccagtattga tgcttttgta tccattgtca
    11161 cttgcgttaa tcttgctttc cttaacagca aaattcttcc aaaagagtcc atttgtctat
    11221 caagtaacga tgttcttttc tgccgttcca gccgtattgg atatgttggc aaattcgcct
    11281 gcattagtga gtcagcaaac tgtcgtagca agcttactag gattttatca tcattatgtt
    11341 ccttttgctg cattaggact tggctgggtc gtcccaacgt tgcttggtta cgctggcagc
    11401 ttgctctttt attatgtata tcgcgtatca ggatataaac aagaagcaag tgagttacca
    11461 gaagaataac caaatcaaac acaatttaac ataagagata ttaaacagga aaaa
//
LOCUS       DAMYXM010000074        11170 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.077, whole genome shotgun sequence.
ACCESSION   DAMYXM010000074 DAMYXM010000000
VERSION     DAMYXM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.077"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            132..1391
                     /gene="lysA"
                     /locus_tag="PNS21_002300"
     CDS             132..1391
                     /gene="lysA"
                     /locus_tag="PNS21_002300"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDI5159989.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1378..2178
                     /gene="dapF"
                     /locus_tag="PNS21_002301"
     CDS             1378..2178
                     /gene="dapF"
                     /locus_tag="PNS21_002301"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDI5159990.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2181..3062
                     /locus_tag="PNS21_002302"
     CDS             2181..3062
                     /locus_tag="PNS21_002302"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDI5159991.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3059..3775
                     /locus_tag="PNS21_002303"
     CDS             3059..3775
                     /locus_tag="PNS21_002303"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDI5159992.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3781..4476
                     /gene="dapD"
                     /locus_tag="PNS21_002304"
     CDS             3781..4476
                     /gene="dapD"
                     /locus_tag="PNS21_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDI5159993.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4492..5493
                     /locus_tag="PNS21_002305"
     CDS             4492..5493
                     /locus_tag="PNS21_002305"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDI5159994.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5490..6851
                     /locus_tag="PNS21_002306"
     CDS             5490..6851
                     /locus_tag="PNS21_002306"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDI5159995.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6811..7962
                     /locus_tag="PNS21_002307"
     CDS             6811..7962
                     /locus_tag="PNS21_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDI5159996.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7937..9106
                     /locus_tag="PNS21_002308"
     CDS             7937..9106
                     /locus_tag="PNS21_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDI5159997.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9281..10660
                     /locus_tag="PNS21_002309"
     CDS             9281..10660
                     /locus_tag="PNS21_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDI5159998.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10941..11170
                     /locus_tag="PNS21_002310"
                     /pseudo
     CDS             10941..11170
                     /locus_tag="PNS21_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3723 a         1950 c         2498 g         2999 t
ORIGIN      
        1 tttaaaaaaa tatttcgaaa tatactagac aattcagaaa attttgttta atattattcc
       61 aataaaattc aaatctttat caaatgaaat tccaataaaa acggaaaaaa agtgaaaaga
      121 ggaatgaaaa aatgacagaa ttgataattg gcggagtcac agcaagcaca ttggcagaaa
      181 agtataagac acctctgtat gtgtatgatg aagaaaagat gagacaaacg atgctgtcat
      241 tcaagcgagg ctttgtatcg aaacgttttg atacaaaagt gctttacgcc tctaaagcat
      301 tccaaacgat tgagatgctg catttagttc gtgaatatgg attcgggcta gatgttgtca
      361 gtggtgggga aatctatacc gctttgcaag ctgattttcc tagagatact atctacttcc
      421 atggtaacaa caagacgcca gaggaactga tctatgcact tgaaaatgat ttgttgcata
      481 ttgttgccga taatttgatg gaagttgaat tattagcaga attaagccaa cgctatttaa
      541 aaaaaatctg tatcatgctt cggttgaatg tcggagtaga agcacatact catgaatata
      601 tcgtcactgc gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa gagtgccaac
      661 aagcccttca gttgataaaa gaaagcagct atctagagtt agaaggattt catgctcata
      721 tcgggtcaca aatttttgat gttactgctt ggttagctga aatcgacaaa ttgacgaatt
      781 acttaaccga ttttgaccaa cctctttcct tgaatcttgg cggagggttt gggattcgtt
      841 atacacaaaa tgatcagccg atcccagtcg aagaggcact aaaaaaactg attgaatata
      901 cggaagaagc tttggaaaaa cgaaaactga ccattcgtca attactgatc gaaccgggta
      961 gaagtattgt tggagaagct ggtacaacac tatacacgat tggtttcatc aaaaagacac
     1021 cacataaaaa atattacttt gtagatggcg ggatgacaga taatatccgt ccagctctgt
     1081 atcaggcaga atacgattgt gatttagcag caaaactgtc ggaagaaaaa acagagaaag
     1141 tgacgattgc aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa gcttatttgc
     1201 cgaaagcaga atcgggtgat cttttagctg tttattccac gggagcttat ggatactcga
     1261 tgagcagcaa ctacaatcga gcgacacgac cagcagttgt ttttgtcaat aatggccaat
     1321 caagactgat tgttcgcaga caagcatttt ctgatctact aaggggggaa gtttcctatg
     1381 atccttcata aatataatgg ctgcggaaat gattttatct tattagatta cgcagaagac
     1441 acagattata gtcaattagc cgcttcttta tgcacaaaag aaaaatatga tacagacgga
     1501 ttgatcgccg taaaaatcga tccgttggaa atgatctatt acaacaaaga tggcagccgc
     1561 gcgccgatgt gcggaaatgg cattcgctgt tttgctcgat acgtaaatga tcagaacatg
     1621 atgaatgaac aggaattcaa cgtagagaca ttagcaggca caagaaaaat tacggtgttg
     1681 gatccctcac ctttttattg cagcgtagat atgggagtgc cagatgattc tccggaaaaa
     1741 atcaaagcta atcaaactta tccaataaaa aaccaggaaa tagagatcaa tggtgaaaaa
     1801 gtaacgatca ctagtttatt tttaggaacg attcataccg ttgtttttgt tgaaaatgcg
     1861 cgtgaggaag tctggagaaa aagaggtgaa ctgatctgtc atcatccttt gttcaaagaa
     1921 aaaaccaatg tcaactttgt agaagtagtc aatgaacaag aattgatcgt ccgtacctat
     1981 gaaagaggag taggttggac acttgcttgc ggtacaggtt gctgtgcctc ttatgtggtt
     2041 gctaaggaaa ctgggaaaat ttcagcagat caggtagttg ttcacttgga acaaggagac
     2101 ttgatgatct ctggtgagga tgcgatacaa atggctggcc cagcagttca tgaatggaca
     2161 agaatattgg aggaatgaag atgataaaag gttcgatcgt ggcgctgatc acgccgatga
     2221 atgaagaggg atcagtagat tatgctggtt tggaaaaatt gatccaattc catttagatg
     2281 aacaaacaga tggattatta gttttaggta caacaggaga atcttctacg ctcacacaat
     2341 ctgaggagga gcagatcctt caactgacag taaagaaagt ggctggccgt gtgccggtga
     2401 ttgcaggagc tggaacgaat aatacgaaag aaacgattga aaaagcgaag cattttgctt
     2461 ctttgggggc agacgcactt ttggtcatca ccccttacta caacaaaaca agtgatgcag
     2521 gactagctgc tcattttaca gcaattgcag aagcctcacc gattccgctt attttgtata
     2581 atgttccttc tagaacaggc atgtcgattc caatccatgt attagtaaat ttagcagaac
     2641 atcctaatat cattggtttg aaagaagcct caggagatat ggcttatgtg atggatgccg
     2701 ctcggttgat tggagaagaa ttcttccttt acagtggaaa tgatgatttg atcctaccag
     2761 taatgagtgt aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa cctaaaatag
     2821 tccatgaatt agtaaaagat acgcaggacg gaagatggca gcaagcaaaa gaaaagcaat
     2881 taaatgcact tgaactgatc catgccttgt tcagtgagac caatcctatt cctgtcaaag
     2941 cagccatgtc attattagac ttaccttccg gacctctacg cctgccttta gtttctcttt
     3001 caaaagagaa aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg gttgaaaaat
     3061 gaaaatcgga ctgattggca gtggacagat gggaaataga gtcgaagaag ttgcacaaga
     3121 aagaggagat acggtccttc ttttacggac ggcacctaaa gaagcaacta cgcttgcttt
     3181 tacggaactg ccagaactgc ttattgattt ttctcatcca gataatttag agaaaatcct
     3241 ttcgtatagc ttatcatatc aacttccgtt agtcattggc acgacaggtt atacacaatc
     3301 acagttccaa gaaataaaag aacatgccaa acatgtacct gtgatgtata gtgctaattt
     3361 ttcgttcggc atcatggtga tgaatcaact aatcaagcaa gcagctgcaa tgttgcaagg
     3421 ctggcagatc gaactgattg aaaaacatca tagtcgtaaa aaagatgcac catctggtac
     3481 tgcaaatatg ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg tatacaactg
     3541 gcaagggaaa aaaagagaag aaaaccaaat tggtgtacac agtattcgtg caggttcttt
     3601 acctggtgaa catgatgtat tgtttgctgc tactgatgaa atatttacga tcaaacatga
     3661 atctttttct aatcgaatct ttgctgcagg cgcagtagaa gcagcggact ggttgaaaga
     3721 tcagtcacca ggttattaca aattagaaga tttattaatg gacaaagggg tgtaaaaaac
     3781 gtggaagctc aagaaatcat ccgctacatt caaacggcat ctaaaaagac gcctgtgaaa
     3841 gtctatctga atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt tggtgaagga
     3901 agcagcttcg ttgtttttgg tgactatgag acgattgctc cggttttaga agctgaatca
     3961 gaaaaaatca ttgaaatcga aatcgaaact gctgcaagat acagcgcagt tcctttgcta
     4021 gatattaaaa aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga tcaagtgtca
     4081 atcggcaaca atgccgtgat catgatggga gcgattatca atattggtgc agtaatcggt
     4141 gaaaacacga tgattgatat gggtgctgtc ttaggtggac gagcgacagt gggcaaaaac
     4201 tgccatattg gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc ggccaaaccg
     4261 gttatcgtag aagacggcgt actggtaggt gcgaatgcag tgattgttga aggtgtccat
     4321 atcggaaaag atgcagtcgt ggcagcagga gcagtggttt tagaagatgt ggcagcagaa
     4381 actgttgtcg gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca gacaaaagaa
     4441 aatacagcat tgattgctgc gttacgcgaa ttatagagga gatggagaaa aatgcgcgaa
     4501 atagatacct gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct caaagtgtta
     4561 gaagaatatg attttccagt aggacgattg aaattattag cttcaaaacg ttcagccggt
     4621 aaagtttgtg tattcaagaa aaagaaacac actgtacagg aattgacaga aacttcattt
     4681 gaaggatatg atttagcgct attttctgca ggtgcttctg tatccaaaga gtttgcgcca
     4741 atcgcacgaa gtcagggact atttgtcata gataattcct cagcatggcg ggaagattcg
     4801 gatattccat taatcgttcc agaggtcaat ctggaagact atacattgaa tcggctgatt
     4861 gcaaatccaa attgttcaac cattcaagcg gtacttccgc ttaaagcttt acaagaagcc
     4921 tttggtatcc agcgagtcaa ttattcgacc tatcaagcag tttctggttc cgggcaaaaa
     4981 ggcattgaag atcttcatcg tacgcaaaat gacgaggaac cagttttgta tccttataat
     5041 attagccaaa cggtgattcc ggaaatcgat cagcctttgg aaaatgggta tacaaaagaa
     5101 gaacagaaaa tgataaatga gacccgtaag attctgcatc agcctgcttt gccagtatca
     5161 gcaacctgcg tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc agttcaactg
     5221 caaaaagagt ttacgacgga agaaattaga gagtgtctca gccagtttcc agggattcaa
     5281 ttagaagatg atctagcagc acataaatat ccgacgagca ttcttgctag aggaacagat
     5341 atggtttatg ttgggaggat cagaaaagac acttctttag agaacggatt gttgttctat
     5401 acgactgccg ataacatacg caaaggagcc gctgcgaatg ccgtacagat tgctgcagct
     5461 ttaatgcaga aaatagcgga ggtggcaaga tgagtaatac gctgaaagta gcgaaattcg
     5521 gcggaacatc gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt gttctttcag
     5581 atcccaatcg ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca gatgatgaca
     5641 aagtaacaga cttactttat ttgatttatg cttaccttca gcatcatgtt gattggacac
     5701 ccctttggaa aaggatttgt gatcgtttca ttcaagtaag agatgttctt gccctgcagt
     5761 tacctattga agatgaacta gcacgcatac agaaacaact ggaagacgaa aagataactg
     5821 aagattattt agtaagtaga ggtgaatatc tgactgctca actgatggct gagtatcttg
     5881 gttaccagtt tattgacgca aaagacctga ttttctttga ctatgatggg aaaatcgatc
     5941 aggaaaaaac agcacaggaa atcacaaaag catttaaaag atatccgaaa atggttgttc
     6001 cagggtttta cggggcgaac cctgcaggaa aagaaaaatt attgggccgt gggggaagcg
     6061 acattaccgg cgcgatactg gcgaagatgt tgcaagcgga aagttatgag aattggacgg
     6121 atgtttcagg aatcatgatg gctgatccgc gaatcatcga tcatcctaaa atcatcgagg
     6181 aaatctcttt tagagaactg agagagatgg cttatatggg ggctaacgtt cttcatgaag
     6241 cagctgtttt cccagttcaa gaggctgata tccccatcca aataaaaaat accaatgatc
     6301 ctttcgctca agggacaaaa atatcgaatc acgaaataga aaaggaaaat ggactgacag
     6361 gaatagctgg acgcaaggat tttctttcca tcactttatt caaacgtcat atgtccgatg
     6421 aaatcgggtt tatctggaaa gcaatgagca tttttgctaa acatgagata tcaatcgaac
     6481 atattccttc agggatcgac aatatcggag tgatcgtctc agcagaagcg attgccgata
     6541 agttatttct catcacgaaa gaattaaaag aaacacttgg cgtggaagaa atcgtcgtga
     6601 tcgaagactt agctttgatc tctgtcgttg ggggtccgca taaagaattg attggattat
     6661 ctgggaaagt gttatcgata ttgaacaaat tagaaattcg cacatcgatc ttatcacaag
     6721 gagcacagga gttgaatctg atcatcggtg taccgaataa tcaatatgaa acagtagtga
     6781 agggtatcta tgaagggatg gtgaatacgg atgctgaaag aatatcgaat ggcactccat
     6841 caaattcctg aaatcgggtt tcatgaattc aagacaaaaa aatatttata tgaacaagta
     6901 aaaaacagcg gggccgttat ccatgaaatc ggcgaaacag gactactcct ttattttgat
     6961 aatcagcaag aagaaacgat tgcttttcga acagatatcg atgcactgcc agtcaaagaa
     7021 gaaacgaatc tctcttttgc ttctaagcat gaaggttata tgcatgcatg tggtcatgac
     7081 ggacatatgg cgatgctttt agggttgact gactttataa ctaaacacat tgaggatctt
     7141 atttataatg tagtactgat ctatcagcca tcagaagaaa tttccggtgg cgcacaaagc
     7201 gttattcagt caggattgtt ggataaatat gaagtacaag caatatttgg ctttcatcta
     7261 tggcctggac tagctaaggg agaagttttc tctcgaccag gacctttgat ggcacaaagc
     7321 agtgaaacag acattatcat taaaggccag tctgcacata ttgcttctag tgaaaaagga
     7381 atcgatagtc tggaggcagc tgtacgattt ttgaagaatg tttatgattt tgaccggcaa
     7441 ttaccggcag agtggaatca tctaatgaaa tttggccaaa tccaaggtgg aacgatccga
     7501 aatgttttag ctggagaagt tattgtatcc ggaagtatcc gttcttacag ttcagatatg
     7561 caaaattatt tgaagagggg attagaccat ttagcagaac agtttcactc tacagctact
     7621 atttcttttc gctataatga cggctatcca gctgtaatca accatagagg cttatatgca
     7681 gcactgttag attcaaacaa actacatgaa ttagatgctc cagttcttca agccgaagat
     7741 tttggcatat atacgcaaca atatccatgt gtatttttct ttttaggtgt cggtgatacc
     7801 cctgctttac atgatccgac gttcgatttt gatatggatg ttttagaaaa tggtgtagaa
     7861 tggtacaaaa cgattctttt tacagaaaat cttcttgtaa aaatcgatag cagttcaaaa
     7921 gaggagcgag taaataatga tgaatttatc caatcgattt agtcctaggc tttctagaat
     7981 agaagtatca aaaatacgac aattcgatca gcaaatatcg tcgataccag atgtgatcaa
     8041 actcaccctg ggagaaccgg attttccaac tcctgaacat gtcaagcaag caggaatagc
     8101 agcgattgaa gaagactttt ctcactatac cggaatgaga ggattagagg aactgcggga
     8161 agcggcttgt atatttcagc aacagcgtta tggattaact tatgatccgc aaacggaagt
     8221 tttgacgaca gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg ttttggaaga
     8281 aggagacaag gttcttattc cagcgcctgc ttattcagga tatcagccat tagtggaact
     8341 agctggtgct gaattgatcc cgatagatac ttctgacaca gggtttgtct gtcagccgga
     8401 acaatttgaa cgagcatttg aacaatatgg atcagctgtc aaagcagtca ttttaaacta
     8461 tcctaacaat cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa tcgctgaggt
     8521 tttaaaaaaa tatccggtat ttgttataag tgatgaagta tatgctgaat tgacttatag
     8581 tggaacgcat atgtccatag ctacttatct tccagaacaa acaatcgtga taagcgggtt
     8641 atccaagtca catgcgatga ctggttggcg agtgggcttt atttttgctc aaaaagccct
     8701 tattgacgaa ctaattaaag tccatcaata tctggtgact gccgctacaa cgatgtcgca
     8761 aaaagctgcg gctgaagcgc taataaatgg ggtgaaagat agcgagaata tgaaagagca
     8821 atatcaaaca cgaagagact atctggtcca gcaattggcg cctttaggat ttgaagtgac
     8881 ccagccaaac ggtgcatttt acttgttttg caaactccca gctgccattc aaactgactc
     8941 ttggcagttt tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc caggaattgc
     9001 atttggccca gaaggagaag gttatatccg gatttcttat gctagcagca tggaaaaatt
     9061 acatgaggcc tgcaaacgga tcaaagactt tttaacagaa cagtgattca tagattttat
     9121 caaaaaaaca atcgttaatt ttgcggtata ttctgcaagg agacgattgt tttttaaatt
     9181 tagttcaaat gaacgcgttt cttttgattc ataactaatg taatgttaag tttaaattgt
     9241 ttagaatgat tttaaaagaa tattcaagga ggtttcatca atggaaaaaa tcaaagttgt
     9301 cattgtaggt gcatctcatg gagggcatca atcgatcttg gaactattga cacgatacgg
     9361 tgaaaatgta gatatcacgc tatttgaagc aggagactat atttccttca tgtcatgtgg
     9421 tatggagctg tatttagaag acaaggtaac agatgtaaat gatgtacgta atttcagacc
     9481 agaaaacttt ccgcaatcca atgttcatat tttaaataat cacgaagtaa aaacgatcga
     9541 tgccgataaa aaaacagtaa cggttactag cgcatcagat aacgaaacaa aagaatatcc
     9601 ttatgacaaa ttgattttga gttctggtgt caaaccaaac aatctgccag taccaggagc
     9661 agatctagaa aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa tcaaagaaaa
     9721 attaaatgat ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca ttgggatcga
     9781 agcagcagaa gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg tgatcgatag
     9841 acctttaggc acttatttag atgctgagat gacagatatt ttagaacaac acctaaatga
     9901 aaacggtatt gaagttttga caaatgcaaa aatcgaagct tttactggta acggaaaagt
     9961 tgaggcaatc aaaacaagtg aaaaagaaat cccagcagat ctagtcattc aagctgccgg
    10021 tgtgaaggca aatacagaat ggttaaaagg gatcgtcgac ttagatgaac gtggctggat
    10081 ccaaacaaat gaatacttgc aaacgaatct tccagatgtg tatgcagtag gagatgctac
    10141 acttgcttat tctatcccag cacgcaaaaa aatgccgatc gctttagcga ctgttgcacg
    10201 tagagaagca cgttatgtag taaaacatct ttttgaagaa gtaccaaaca aaccgttcgg
    10261 cggagtcgta ggttcctctg cattaagtgt ctttgactat cattttgcag caagcggatt
    10321 gaatagcttt acagcagcaa aagcaggagt agatgtacga gtgtcttatt atgaagatac
    10381 gttacgacca aaatatgtac cagaagaaca aggaaatcca aaagtgtcca tccaattatt
    10441 ctcggatcca atgacccatc aattgctagg aggagcagta ttatctacct atgatgttac
    10501 aggtcaagga aatgtattgg cactagcgat ccaaaacaaa ctgactttag aagaactagc
    10561 agaagctgac ttcttcttcc agccaggctt tgaccgacaa tggagcatgt tgaatctagc
    10621 cgctcaacaa gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa tatttgaaaa
    10681 gaggttgtga aaaagctgcc ttgcatcaag taataagaac agccaagtaa aaacagcttc
    10741 ttatgttttt aacttggctg gacttattac cgcagatgca agcttttgaa caccgtttat
    10801 tttcaaaaaa gggactgtga cacgatttgt gtaccgaccc ccaaaagtta gaccaaaaaa
    10861 tctaactttt ggaggtcggt attttttatg gttaaatata cttttgaact taaattaaag
    10921 atcgttcatg attatttgga tggcaaaggc gattggaccg taaaaatcca gatcaagaga
    10981 ttgaagcgga aatgcttaaa attcgggaga agcacaagga ttatggctgt ttacggatga
    11041 ccaatgaact aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag cgcttgatca
    11101 agaagctagg aataaaagta acggcataca cgcgtaaatc acgtcgctac aattcttatc
    11161 gaggaaaagt
//
LOCUS       DAMYXM010000075        10993 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.078, whole genome shotgun sequence.
ACCESSION   DAMYXM010000075 DAMYXM010000000
VERSION     DAMYXM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.078"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..340)
                     /locus_tag="PNS21_002311"
     CDS             complement(<1..340)
                     /locus_tag="PNS21_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI5159999.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVR"
     gene            complement(1671..2336)
                     /locus_tag="PNS21_002312"
     CDS             complement(1671..2336)
                     /locus_tag="PNS21_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5160000.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2516..4498
                     /locus_tag="PNS21_002313"
     CDS             2516..4498
                     /locus_tag="PNS21_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDI5160001.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4502..5140
                     /locus_tag="PNS21_002314"
     CDS             4502..5140
                     /locus_tag="PNS21_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5160002.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            5168..5596
                     /locus_tag="PNS21_002315"
     CDS             5168..5596
                     /locus_tag="PNS21_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160003.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            6053..6964
                     /locus_tag="PNS21_002316"
     CDS             6053..6964
                     /locus_tag="PNS21_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDI5160004.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            7418..7732
                     /locus_tag="PNS21_002317"
     CDS             7418..7732
                     /locus_tag="PNS21_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDI5160005.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            7748..8134
                     /locus_tag="PNS21_002318"
     CDS             7748..8134
                     /locus_tag="PNS21_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDI5160006.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            8163..9548
                     /locus_tag="PNS21_002319"
     CDS             8163..9548
                     /locus_tag="PNS21_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDI5160007.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            9551..9703
                     /locus_tag="PNS21_002320"
     CDS             9551..9703
                     /locus_tag="PNS21_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDI5160008.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            9726..10931
                     /locus_tag="PNS21_002321"
     CDS             9726..10931
                     /locus_tag="PNS21_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDI5160009.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT         3727 a         1799 c         2141 g         3326 t
ORIGIN      
        1 ggcgaacata attctttcca aaagttgaga aatgggggat tttctcctcg aaggagtagc
       61 ctaagaacca gcggtaagcg acattggtat cgacttcctt aatggtttgt cgcatggaac
      121 ggataccgaa taaatactga ataaacacca atttgatcag aatcactgga tcgatacttg
      181 ggcgacctaa atcggagtaa gtggcttcga cgattggata aataaattca aaatcaagcg
      241 cggcatcaat ctttcgaacg agatgatctt gaggaactag atcatcaatc gtaagcatcg
      301 caaattgatt tctaccattc gtattgtttt tagacatcat agctatcact ccttgcattc
      361 gttttcataa atctattata aaagaaaaga ctgtagatat catccataaa atggactttg
      421 tctacagtct gaggactacc tgtattagtc ctcttttttg accagatttt gaccagtcta
      481 ttttacattc taatatattc atatgagcta attttatgat taaatggtaa gtgtttcctt
      541 gtttgtttta gtttaaacta gtgtgtaatt taaaaatcta tatggaaggg tctgtggggt
      601 aatacttaaa taaaggctag gaacctgata acatcaaggt tcctagcctt tttataataa
      661 aatgaagcga aaataatttc ttttaaagag ccgagattat cagctatata agagtgaatt
      721 tgatgcaaaa aacagagccg aaacagtctt ggtgttcgta gctctgctta tatgtttcga
      781 atttaagaca aagagttggc aaaagatgtt gataaggaag ataagataac tgtgttatat
      841 aaactaggta actaactgta acaagctaat tttaagagat tcttattaga agacgtttag
      901 gaatataact aagaaaatga ttatacaggt aattgtcatt tcaataatgt tttctaattt
      961 tcgcgtcttt gttttaagaa aaaggccaga atcagttgga aaaaagttcg tacgatagtg
     1021 aagaggggca aaatttccta ctaatattcc cactaacaag agataaaacg gccaggttgg
     1081 tgtgattcct ctataaaaaa atgcccatgc taccgaaatc ccatagatta acaactgaac
     1141 aatttttaca atgaaaaaat tgttctgcca ttactcaaag tctcttcctc cttgtatcgt
     1201 ttatttgttt cattttatca ttatgatagg cgcttgaaca atccattttg tattttattg
     1261 agtactcttg gataaactac cagcgattat catatttatt tatgttactg taagccatca
     1321 tataaaagta aatgagcaga tcatagaaat ataaaaaaga actaaaattg atctaaatta
     1381 tgtgcagctt gactacgtac caatctatat tacttactag attggtcgtt aatctattcg
     1441 gatcgaatga gcaaaatatt tttgtgaatg tccatccttg attatgattc caaaaagaat
     1501 cattgggagg ctgtggcgta aatattatac tataagccag aaatacctat cattctgaac
     1561 ttttttattt aggaactttc atctcaaggc tttttcaatt aatttgtacc cctgtttttt
     1621 ggataagatc atgtgcattg attttttcta tctctacgtt catctcctac ttaatgccta
     1681 tctggctcct gagggcttct ttttaaggcg tcgtcatagt cttggctgag gatttcttgg
     1741 taatctgagg gaatggcttc ccaagtgagc aggcgaacgt tcatgacgtg ttttcctttg
     1801 tgtttgatta cagcatggtt tttctctttt tctacctctc cagcgtgaaa agacagccat
     1861 gtaccatggc caaagatatc attacatacc caacgttcga tggaattgtc cacgtatcct
     1921 acagggagtg tggtatagcc gatttgtggt ttcaaaaaag gattgaatcg atcgatcgtt
     1981 tcatttttgt attgataatt gataagaaaa agatagacca aacaaaagac gagcagaaag
     2041 aaagatagtg tctttttgag tgagaagggt tgttttgatg aataagattt cttgttaaca
     2101 agaggttcat ctggcaatgt tggttctatt gattcggtag ctgattcaat agaaggaaaa
     2161 tcaatcggca gacctaaagt atgatgtaaa agtgacagca tttcaaggtc tggcattgta
     2221 cgagaatttt cccaactaga aatcgtttta tcggaaacac caattttttc gcctaattct
     2281 ttttgcgtaa gcttacaatt tctgcggtaa gttcgtattt tttctccggt gttcatcttc
     2341 ttctcctttc gattctacag tatatagatt tattctacat gacctataca cttgatagat
     2401 acgttttttt gatgaaaaac ggtatgattt tatcaaagaa tcatttaagt aagggagagt
     2461 ggtgataatc aaaaaaaagt tcacagtttt actcacaatc atagtgaccg gtatcatgag
     2521 tggttttgcc ttggttagta taaataaaca ggtactctat cggacaaatg aatttttcca
     2581 tcaaacagat acttatactc cgattcgatt gccacaacat tttattgggc aagaagaata
     2641 tgagcagcta atcatggtat ttgaaaatgt ctcatcagaa actgagcttt cttatatgaa
     2701 aaaaacagta aatgaatcca atgaagtaga tacatcttgg aacttcaaga tacagccaaa
     2761 gcttgaaatg acattgtatt atcaagggga tgtcaaatct tctcacgaga aggtacccta
     2821 ttctaatacc tcctttcgcg taacgtccat tgaagaatcg atcaaaataa aagattttga
     2881 aggtgaattt ttcttgatga cgagagaaaa agaagcctat gaacggttca tttcactatt
     2941 cactgatcga ttcaatcaag catttcacac tgattatgcc actaatattt ttgaagactt
     3001 tgaaaatagc tattcgtatt cggttatttc aaatacagac gtatataatt atcgagtgta
     3061 tttaactatt ggattgattc ttttattcct tttgttatca tcctttttct tattgatgaa
     3121 taaagaaatc agtttattgt tgaataatga cttttcatta catcaagtat tgtggtgttt
     3181 attggggaaa aagaaaatca ttattacatt cataattgca ggaatcattg gtggttttat
     3241 ttttatgtat tataaggagc aatttcttta tttgttccag atgtacgttt tcttgttcag
     3301 cggaatatat ggtatctctt tgatttctct tttattagtt gaaaaaatcg cttcgttaag
     3361 agaagcgttc aactatatcc aatacattct ttttacattt ggattgttgt tttctcttct
     3421 tttgaccgtt gccaatgtcg aattagcaac tatcgttttt tcttcttcac agttagctgt
     3481 ttcccatgaa ccaccaaaag aaatcgctgg tgaggactat catgtatttt atccagtaac
     3541 catcgggaaa aaccacgtgg attttattta ttccaatcgt tttgcatcag cagcagatca
     3601 ggaattatat gaaactttga ataaaaacgg aagtatatta gtcaatgtat cagattactt
     3661 gaatgaagaa aatgaacagt ttggcagagg gataaaaata aatctgaatt atcttaagaa
     3721 atttcctttg attgatgtct ctggaaggtt gatccaaata accgaaaaag aaacgcaccc
     3781 tgttatattg atccctgaac gatatagaca tacagattta aaaaatgacc tggcgcaaat
     3841 aacagaatat tatcaagaaa tcagtgaaaa agaaccgaaa tttctattta taaaaaacaa
     3901 tcaacccatt tatacattta ttccttctat cccctggatc gaacattatc cggttgttga
     3961 aatactgaca ttgaaaaaca gtatgtattt ggaaagaaat attctcagcg gagaaatcta
     4021 tccaccgtta aagatcaaga taggctcttt gtcgaaagaa aggttatttg agctgattga
     4081 agaaagtcag ttacgtgata acttgcagtt atcatttccg tatactcaga ctgaagagat
     4141 agctgtcaaa gtgttaagca gatcatttca ttacttgata cagatatttt tactcacttt
     4201 cttttctttc ttcttgttga gctatgtcat gttatgcata tattttgtct acaattgtcg
     4261 gaaaatcgct atctttcgtt caagtggtta ttcgctcttt gaaacgtata aagatttctt
     4321 catgatgaac ctcatcaaat ggggaacgac aagtgtaatc ttcctttttc ttatagaaag
     4381 agagcctaaa tatcttttta atattttttt ctttagcttc attgatttta ttctttcagt
     4441 tgtttttatt ttgtctacag aaaagaaaag tcagttattg ctgatgaatg gaggttaaca
     4501 attggagccg ttgatagtga taaaaggtgc gaaaaaaatc attcgaaaca aaacgattct
     4561 tcatttggat cgtttcactg cggatgctgg ggatcttgtg actgttgttg gagaaagtgg
     4621 agcaggtaaa agtacgttat tgaatatatt aggattgaat gatcagttca gttcaggaca
     4681 attctatttg ttcgatcaag agacgaaaca attaactggg agagagaagc gcttgataaa
     4741 aagaaatacc atatcttttc tttttcaaga ttttgggttg attgaatatg aaacaatcga
     4801 ttttaattta gatattggat tgaagttttc gaatttaaat gtttcagata agcgaacagc
     4861 aaagtatgag gcattgaaaa aagtaaatat aaacaagaag ctaagtacgc ctatctacac
     4921 attatctggt ggtgagaagc aaagaatcgc ctgtgcgaga gtcttattga agaatagcaa
     4981 aatcatttta gccgatgaac cgactgggtc attggataaa agaaataagg ataaagtagt
     5041 tgaaatctta tcgcaacaag cttcccaagg tacgttagtt atcgtagtca cgcatgatcc
     5101 atatctgacc aaaataggaa acaagaagat catgctttaa aaatcaaaaa ggaaaggatg
     5161 tcgttttatg aaaaaagcag ttattatttg tgcactaggg ataggttttg ttggaggaaa
     5221 cgctatgtca tctgaagtgg aagcaaattc agttcacggt gaaatctatt acaaatcttt
     5281 aaagccagga gagattcatt ttgatgctaa aggtaatatt attaagtcaa atttaccagt
     5341 agaccgggaa atttctagaa cagcaattaa tcatagctct ggaagatggg tttattatag
     5401 tgatacaact tgtacgagaa aaacaggtca ttctaaccac tatagtcgtg tctataatcg
     5461 tcacggtgca aaagcgaaag ttggaaactc tattcgatca gcaaatgcaa caaaaggagt
     5521 atgggcctat gcaagtgcga caggaaatag aactgacaca ttctcatgtt ggtacaatcc
     5581 aaccggttgg tattaagtat ccaaaagttt atataaaata gaaaaaacat ataatgaaac
     5641 agcgttaaaa gttatttttg gtagttttaa tatattcatt tttagttgta taaaaatata
     5701 tcataataaa aacaacaaaa aaatgtctgg aaaatcgaac ataaaagtgt ctgatttgaa
     5761 tatcccagca gcggaagaag aaaaacaaaa tcgataacat ttgttctaag aattagttac
     5821 gttaaatacg gaacgcaaga tagacttgaa aaacggtaac aaaagaagta gtatattaag
     5881 gtgtgatgtg ctacttgaca tttattctgc tgagcgaact ttctggattg tgtagcctta
     5941 tatctattca ggacgtatcg agcagattag taaaggtgtt cttgatgatt ttcatcttga
     6001 acacctttgt ttttagcaat tcgcaaaaaa cgaaaagtag gagaattgat aaatgaaaaa
     6061 aagattttta gcacttgcca tcgtactagg cactggctta ttgtcaggct gtacgaatgc
     6121 cggtgaaaaa acagccgtta gttataaagg cggtacaatc agcgaacaag aagtaatgga
     6181 tagcttgaaa aaaatgcaag gagcagattc cgctgtgcaa cagttgatcg tttatcaagt
     6241 atttgaagat aaatatggag atgacgtatc aaccaaagaa atcgattctc aatatgatca
     6301 aacaaagaaa caattgggtg attcatttga ttcacaattg aaatctgcag gttacacaga
     6361 acaaacattc aaagacagta tcaaacaaag tctagccttt caagaaggtc taaagaaaca
     6421 tatcaaattg accgatgaag atttaaaaac ggcttgggaa tctttccatc cagaagtgga
     6481 agctcaaatt atccaagtag caagtgaaga tgatgcaaaa gatgtgaaga aagctgctga
     6541 taagggagac gatttctcta aattagccaa agacaagtca actgatacta cgacaaaaga
     6601 agatggcgga aaagtgaaat ttgattccac tactacaacg gttccggcag aagtcaaaga
     6661 agcagccttt aagttgaaag atggtcaagt ttctgatgtg atcacttcta ccaatgcttc
     6721 aacttatact acggaatatt atgtggttaa aatggtgaaa aaccaaaaca aaggcaacga
     6781 catggataaa tacaaaaaag aattgaaaga aattgcaaca gacacaaaat taagtgacag
     6841 taccttccag aataaagtaa ttggcgaagt attgaaagac gctaatgtaa aaatcaaaga
     6901 caaagacttt gaaaatgttt tgagtacatt cacttcagat tctagtacag caagtagtaa
     6961 ataagcggta taaataaaaa atatgaacta tttaaatcaa taaaactgtt catctaaatc
     7021 tttttattag aactaaatca acagtagttt aattactact tgatttagtt ctttttctac
     7081 aaaaaatagc ataaaaatct agttatccgc ataaaaactg gacttatcac actttatcaa
     7141 ggtcaaaacc actcaattta ctactaattt actacttatg aatgagcttt gatacgacga
     7201 tttatccttg aaaagtgaag atataaagat acttccaata aaatttgaat atttaatagg
     7261 tagacacttc aaaaaatgag gtgtctattt ttttacccga ttttgaaagg aagtgaactt
     7321 atgaaaacaa aaaatcaaga atcaaaaggt cgttccccac tctttaagac catcaaacat
     7381 tcattcagcc aataaaaaag aaaggatagg taaaaatatg gaacttaaat ttgtgattcc
     7441 caacatggaa aaaacattcg gcaatttaga atttgctggc gaggataaag tcgttcagcg
     7501 aagaatcaac ggacggctaa ctgtcttatc aagaagctat aatctctatt ctgatgttca
     7561 aagagcagat gatattgtgg tggtgcttcc tgctgaagct ggcgaaaaac atttcggctt
     7621 tgaggaacgt gtgaagttag tcaatccacg tattaccgca gagggctaca aaatcggcac
     7681 tcgtggtttt acaaattacc ttttacatgc tgacgacatg ataaaagaat aaagaaagag
     7741 aggaaaaatg atgagattag caaatggcat tgtattagat aaagacacga cttttggaga
     7801 attgaaattc tctgctctac gtcgtgaagt gagaatccaa aatgaagacg ggtcggtttc
     7861 agatgaaatc aaggaacgta cctatgactt aaaatccaaa ggacaaggac gcatgattca
     7921 agtaagtatt cctgccagcg tgcctttgaa agagtttgat tataacgcac gggtggaact
     7981 tatcaatccc attgcggaca ccgttgctac tgccacctat caaggagcag atgttgactg
     8041 gtatatcaag gcagacgata ttgtgctgac aaaggattct agttcattca aagctcaacc
     8101 acaagcaaag aaagaaccga cacaagacaa atagtcgcta ggtagaaagg agactttttc
     8161 gcatgaaaca gcgtggtaaa aggattcgcc catctggtaa agatttagtc tttcatttta
     8221 cgatagcgtc actcctgcct gttttcctgc tggttgtcgg actgtttcat gtgaagacaa
     8281 tccagcagat caactggcag gattttaacc tatcacaagc agataagatt gacattccct
     8341 atttaattat cagtttcagt gtcgcaattc ttatctgctt gctggtagcg tttgtattca
     8401 aacgggttcg ctatgatacg gttaaacaac tttaccaccg tcaaaaactg gcaaagatga
     8461 tacttgaaaa caagtggtat gaatctgaac aggtcaaaac agagggtttc tttaaagata
     8521 gtgctggtcg tacaaaggaa aagataacct acttccctaa aatgtattat cgacttaaaa
     8581 atggcttgat acagatacgg gtggaaatca cgctgggaaa atatcaagac caactcttac
     8641 acttggaaaa gaaattagag agtggcttgt actgtgagct gacggataaa gagttaaagg
     8701 attcctatgt ggaatatact ttgctctatg acaccatagc cagtcgtatt tctattgatg
     8761 aagtagaagc taaagatggt aaacttcgct taatgaaaaa cgtatggtgg gaatatgata
     8821 agctccctca tatgttgatt gctggtggta caggtggcgg taaaacttac tttatactga
     8881 cactgattga agccttgctt catacagatt caaaactgta tattcttgac ccgaaaaatg
     8941 ctgaccttgc ggacttaggt tctgtgatgg caaatgtcta ctatagaaaa gaagacttgc
     9001 tttcttgcat tgaaacattc tatgaagaaa tgatgaaacg tagtgaggaa atgaagcaga
     9061 tgaagaacta taagactggc aaaaattatg cttacttagg tctcccggca cacttcttaa
     9121 tctttgatga atacgtcgct ttcatggaaa tgctgggaac aaaagaaaac accgcagtta
     9181 tgaataagct gaaacagatt gtcatgttag gtcgtcaagc tggcttcttt ctaatactgg
     9241 cttgtcaacg tccagacgca aaatatttag gcgacggaat ccgtgatcag tttaatttca
     9301 gagtggcttt aggtcgtatg tctgaaatgg gctatggcat gatgtttggc agtgacgtac
     9361 aaaaggattt cttcttaaag cgaatcaaag gtcgtggcta tgttgatgta ggaacaagtg
     9421 tcatatcaga gttttatact ccccttgtac caaaaggata tgatttcttg gaggaaatta
     9481 aaaagttatc caacagcaga cagtccacgc aggcgacgtg cgaagcggaa gtcgcaggtg
     9541 tggactgatc ttgctggctg gtgtggcaat agccacgcca gcacttaacc ccccgtatct
     9601 aacagggggg tacaaatcga caggaaacag tcaaaaaaac attagaaaat cctttggtta
     9661 caagggattt acaaaatttc agcgtatgtc aaatgggctt taaaagttga catacgcctt
     9721 tttgattgga gggattttta ctgaatgaac aaacttggtt acagcattta aaagaaaaac
     9781 gcttggctta tggactatct caaaaccgtt tagctgttgc gactggtatt acaaggcagt
     9841 atctaagcga tattgaaaca ggaaaagtca agccatcaga ggatttacag cagtcccttt
     9901 gggaagctct ggaacgcttc aatcccgacg ctccccttga aatgctgttt gattatgtaa
     9961 ggattcgctt tccgacaaca gacgtacagc aggtggtcga aaacatctta caactgaaac
    10021 tgtcctattt tcttcatgag gactatggtt tctattctta ttcagagcat tatgctttag
    10081 gcgacatatt cgtcctttgc tcccatgaac tggacaaagg agttctggtg gaattgaaag
    10141 gtcgtgggtg cagacaattt gaaagctatc ttctggcaca acaaagaagc tggtatgagt
    10201 tctttatgga cgttttggtg gctggcggtg tgatgaaacg ccttgacctt gccattaacg
    10261 ataagacagg gattttgaat atccctgtac tcactgaaaa gtgccaacag gaagaatgta
    10321 tctccgtctt ccgcagtttt aaaagctatc gcagtggcga actggtacgc aaagaggaaa
    10381 aggaatgtat gggaaacacc ctctatatcg gttcattaca aagtgaagtt tatttctgta
    10441 tctatgaaaa ggactacgag cagtacaaga aaaatgatat tcccattgaa gacgcagaag
    10501 taaaaaaccg ttttgagatt cgattgaaaa atgagcgtgc ctattatgca gtccgtgatt
    10561 tactcgtcta tgacaatcca gagcataccg cctttaaaat tatcaatcgg tatatccgtt
    10621 ttgtagataa agacgattcc aaacctcgtt ctgattggaa actgaatgaa gaatgggctt
    10681 ggtttattgg gaacaatcgt gaacgattaa aactaaccac aaaaccagag ccttactcct
    10741 tccaaaggac gctgaactgg ctatctcatc aagttgcccc gaccttaaag gttgcgatta
    10801 aacttgatga aatcaaccag acgcaggttg taaaagacat tctcgaccat gcgaaactga
    10861 cagaccgaca caagcagatt ttgaagcaac agtcagtaaa agaacaggac gtgataacaa
    10921 caaaaaaata actcaaatac aaattcattg aatatagaga ggagaacatt tttatgaatt
    10981 ttggacaaaa cct
//
LOCUS       DAMYXM010000076        10685 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.079, whole genome shotgun sequence.
ACCESSION   DAMYXM010000076 DAMYXM010000000
VERSION     DAMYXM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.079"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(18..515)
                     /locus_tag="PNS21_002322"
     CDS             complement(18..515)
                     /locus_tag="PNS21_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDI5160010.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(645..1355)
                     /gene="budA"
                     /locus_tag="PNS21_002323"
     CDS             complement(645..1355)
                     /gene="budA"
                     /locus_tag="PNS21_002323"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDI5160011.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1368..3032)
                     /gene="alsS"
                     /locus_tag="PNS21_002324"
     CDS             complement(1368..3032)
                     /gene="alsS"
                     /locus_tag="PNS21_002324"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDI5160012.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3237..3899)
                     /locus_tag="PNS21_002325"
     CDS             complement(3237..3899)
                     /locus_tag="PNS21_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDI5160013.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3909..4724)
                     /locus_tag="PNS21_002326"
     CDS             complement(3909..4724)
                     /locus_tag="PNS21_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDI5160014.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4986..5429)
                     /locus_tag="PNS21_002327"
     CDS             complement(4986..5429)
                     /locus_tag="PNS21_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HDI5160015.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5563..5901)
                     /locus_tag="PNS21_002328"
     CDS             complement(5563..5901)
                     /locus_tag="PNS21_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI5160016.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5889..6266)
                     /locus_tag="PNS21_002329"
     CDS             complement(5889..6266)
                     /locus_tag="PNS21_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDI5160017.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6490..7683
                     /locus_tag="PNS21_002330"
     CDS             6490..7683
                     /locus_tag="PNS21_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5160018.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7849..8271)
                     /locus_tag="PNS21_002331"
     CDS             complement(7849..8271)
                     /locus_tag="PNS21_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5160019.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8861..9316
                     /locus_tag="PNS21_002332"
     CDS             8861..9316
                     /locus_tag="PNS21_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160020.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(9478..10650)
                     /locus_tag="PNS21_002333"
     CDS             complement(9478..10650)
                     /locus_tag="PNS21_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDI5160021.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
BASE COUNT         3212 a         2143 c         1812 g         3518 t
ORIGIN      
        1 tgtttcaata gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct
       61 aataaaagat ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt
      121 actccgcaat cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg
      181 atttctgatt gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca
      241 acgatcaatg tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata
      301 cgtgtgcaag aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa
      361 taatatcctg caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct
      421 aaatttgcta accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt
      481 tcgtatgcat cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct
      541 atggagaaga ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga
      601 gatacgtcag gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg
      661 atttctgctg ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact
      721 ggaaaatgct gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca
      781 aaatccatta catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa gtgaaatccc
      841 gctgcagcaa ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggaca
      901 ttgctttgtt ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt
      961 tcttgcttcg gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc
     1021 aaaaataggt tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct
     1081 gcagtttttg agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg
     1141 gtaagttcac ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg
     1201 ccatctaaaa agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct
     1261 ttttctaata tatgagcaat cgtttctgtt ccatccatca agccagccat caaagctcct
     1321 aatgtcccgt gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga
     1381 tctggtaaga ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata
     1441 attacgggtc cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt
     1501 gtagctcgga ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca
     1561 aagtctacac ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag
     1621 tgtccatcat tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa
     1681 tcctgggcag agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga
     1741 cgcaccaatg cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta
     1801 ctgaataaca aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga
     1861 ctacctacat ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata
     1921 acttctaaag gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt
     1981 aattctgctt gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct
     2041 aatgaacctg tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc
     2101 tgcataaaag gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt
     2161 tccgcattcc agtttcttgc ttcatattcg attggatcat agccaatagc aataactaga
     2221 tcacttcgtt tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag
     2281 aaatgcgctt ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc
     2341 aatgctgtct ttccaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc
     2401 atacctacta atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata
     2461 tcatctgcag aagcagaacc taatttagga tcgctcaatg gcttgatgac attcccttgg
     2521 acaggtgcat ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc
     2581 ttagccatac gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct
     2641 gcactgtatt ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac
     2701 ttcaatagat cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct
     2761 gtcgctgtta ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact
     2821 actcctggct ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg
     2881 tgacgagtca cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt
     2941 tttgcacctg gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca
     3001 gatttcgaac cttgtttcat tttttcgtcc attgaacatc gtccttttta tgctttattt
     3061 ttaattatta gttagaaata atcacaaata attgatacag tttctaaacc tgtttcattc
     3121 aaaagtatag cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa
     3181 tgacagtttt tacataaaaa ctgtcattct taattgaaag tttcaattta taaagtctac
     3241 agatccatag ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa
     3301 ggacctagta gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat
     3361 agtaaaaaat ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt
     3421 agaacttgat ttggtttcga cgctggattt ctcacctgaa aataccagta gttgatgcta
     3481 aaggaaactg ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa
     3541 aaacgtataa aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc
     3601 caataaaaaa atccctttag ccaaaattgg cgaaacgtct ttatgaacaa acgtactggt
     3661 gaaaaacttt tttcttttgc taattcttct gtcataccta tcatcacgat gcaacctgga
     3721 ccaataaaaa gtaaagaaaa aataaaggaa aagacattcc ttccatctat cgacaaaagg
     3781 aacgcagctg ctatatacaa gatattgaca caccaaaaac aaaggctact tgccaatgat
     3841 tgatacaacc aataaaagaa ttccatataa acatttttgt caaaacgctg ctctttcata
     3901 atcacctcct attccttcat tcctgatgaa gcgatccctt ctacaaaata tttttgcatc
     3961 gctaaaaata taaaagcaac aggaagaaca gaaacaactg aggcagccat gattaatgcg
     4021 tactctgcat caaatagccc aataaacatt ttcaacccta actggattgt tttattttcc
     4081 gtagaagaca aataaatgaa tggccccata taatcgttcc atgtattaac gaatgtgatg
     4141 atcgtcaaac tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg atagattccc
     4201 cactctgata agccatcgat ccgcgcactc tcacataaag aatctgggat actactgtag
     4261 tattgcttca acaagaacac accaaaagca ttgaacgctt gtaatagcac caacgaccac
     4321 aacgtatatg ttaaacccac ttttctcatc ataatgaact gcgggatcat ataagactgc
     4381 caagggacag caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc tttaaaattt
     4441 agtttagaaa acccgtaagc agcaaaacta gaagtaaaca actgaatcaa cgtgatcaca
     4501 acgcttaaaa aagctgtatt cttaaaaaat gtcactaaag gaatacgaga ccaaataacc
     4561 agataattct ctaaatgcca agttttaggg atccattgaa tcggaatcgt gaacacttca
     4621 ttatttgctt tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt caataaagct
     4681 aatacaatca gcagaatcat acccaaccat tgaataaagg gcatgttttt tgaacgaatt
     4741 tttgcttttt ttctttcatt tcgaacacct ccaaagttat aatcttcatt tctttgtaat
     4801 gattacaatc cagaacaacg gttattatag ctgattcatt tccgttcttc ttatgtttgt
     4861 aagcgcttat ttaacaagtt atgtaaatct ctttattttt tttgatttaa aaatgaaaaa
     4921 atacaaaagc aagactgtca tacgtctcgc ttttgtattg aaatttcttc gattagagat
     4981 acttattact gttcaaaagc ttctttgaaa cggtgaatca attcattgac tacaggtgat
     5041 aaaatccgat tctttttcca gactagcatg cagttcgttt caatcattgg gtcaagcgga
     5101 acgaatgtca ctcgctttga aagatgactt acagcacctt ctatgactaa agcagcacct
     5161 acctgattct caattagcgc aaatacattg aaaatcaaat tgtacgttcc tgtgatcagc
     5221 aactgctcct tttttatgcc taaccagtca gcaaacattc gctgtatttc tggacgcttt
     5281 gagatcaaaa gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt ttttgctaaa
     5341 acagaatttt ctaaaatgag caatccccat ttttcctttc ttggtaacaa aatcgtttcg
     5401 tatttctctg tatctactgg atctagcaaa atcgctaaat ctaacaggct tttttctagt
     5461 cactccgtga tatcatcact tgttccgctg tggatatgaa attgaacgct gggataatca
     5521 cggatcaact tttctagtaa tagtgccaat gtattcgaat tatcagcttc cacacagccg
     5581 atcgtaaatc ggccgctgaa taattgtttt tttctgttct cgaattcttg agtggtttga
     5641 ttcgttaaat ctaaaatttc ttctgctcgg cttttttaga aaataacctg cttctgtcaa
     5701 gatcattttg cggttttcgc gtacaaacag ttctgttcct agttcctctt ctaactcctt
     5761 cagttgtcgg cttaaggttg gttgtgtaag atgtagaact tctgcggctt gagagattgt
     5821 tccttcttct gcaattgtcc aaaaatattt tagtaagcgt aactccatag cgatgctcct
     5881 tgattttttt acttgataca ttccatttta tttctagata acacacgatc atatttcttg
     5941 atttttcctt gtaaacgttc caaacattct tgcatttcag caattttttt cgtcaaaagt
     6001 tcactttctt caatcaaaag cttacgccgc tcaaatacag ttgcttctcc ttcgctagat
     6061 aggcgaatat attcaatcaa agactcgatc gataatccgg catttcgcat acagatcgta
     6121 aagtcgaccc attttaggtc ttcttctgta taatcacgga ttccgccttt tgtccgatta
     6181 actggtggaa tcaacccgat tcgttcataa tagcgcaatg tatcggccga aatgcccttt
     6241 tcttcactga cttgtttgat attcatctga tcactctttc tcttaatttc ttgccacttt
     6301 acttatgtat tttatttatt ctacaacttt aagtgtactc caagtcaata ctcatttcta
     6361 ttaaaactaa gctggattat ctatatcggt tgtttgtgta ttatttcaaa taaaaaagtc
     6421 ttgttttttt attctttttt ggatatactt ttaacaattt agggaaattt taggaaaagg
     6481 ggtataaaaa tgacaacgta tcagcgaaag ttattattat cgacatcggc gggaattgct
     6541 ttagagaata tggatattat gtttttagct ttttcattat cttcaatgat tacttctttt
     6601 catattagtg gtacgcaagc gggtttcatt tcaacgatca ctaatttggg gatgctcatt
     6661 ggaggtattt tttttggcat catagctgat aaatatggac gtgtgaaagt attttctcaa
     6721 acggtccttc tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa catctacttg
     6781 gtttatctct ttcgctttat cgcaggtgta ggagcgggag gagaatacgg ggcgtgtatg
     6841 tccttgattt ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc ggttgctggt
     6901 attggtgcgc aggtcggtgc ggctttggct gcgattttag ctgctgtgat cattccaacg
     6961 tttggttgga agatgttgta tgtcgtaggt gtactgccgg tgttgatggt cttgttcatt
     7021 cgtcgcggct tgactgaacc aaaggaattc aaatctgtac aatctgccga aaagaaaacc
     7081 aaacttagca gactgtttga atcaaaacat cttgcttggc aaacatgtgg ccttagtatc
     7141 atggtaactg tccaaattgc cggctatttt gggttaatga attggctgcc ttcaattatg
     7201 caaaatcgat tagggctttc ggtttctggg tcttcattgt ggatggtcag tactatttta
     7261 ggcatgtctt tggggatgat gacttttggc ataatcatgg ataaattggg gcctaaacta
     7321 gccttttctt tgtttttaat ttgttcatcc ttatctgtct atctacttgt gttagcccat
     7381 agccagtgga gtttggtagc agccgctgtg atagtcggct actttatcaa tggcatgtat
     7441 ggtggatatg gcgcagtgat tagtagtttg tatccaacag aaatccgcgc gacggcgaat
     7501 aatttcatta tgaaccttgg acgagctgtt ggtggttttt cttctatcgt catcggtttc
     7561 ttgatggatc attatagttt aactgccgta atcttgtttt tgagcggtat ttacctagtc
     7621 agtctgctgg tcttgacaac tcttactggt gttacagcat taagggaaag cttgcagcat
     7681 tgatagttga gtctgtaaat gacaagaaag aggtgtggca aaagcttgca tctgcggtaa
     7741 taagaccagc caaatcaaaa acatgagaca ctgtttccgt ttggctggtc gtattacttg
     7801 atgcagggca gctttttcaa ctgctaattt ccttctatct ggcggctact attctttgac
     7861 gtccgtcatt tcaaagtact ttactaataa cttagcataa cgaaaaactg tggatacact
     7921 cgtatattga gcgtctgcat aatcttccaa atttttctca ggatgttgat acatgaattc
     7981 aatcaaatga taattatatg attttctgta gaaatgaaga taaataatgt agattgggaa
     8041 gttctcctgc ttttccatag aaaagcgccg attattctca ttcttcaaaa tgaccttatc
     8101 aggatatttt tggaacaatt cttctaaatc tgttagataa ttcaagatag acttacttga
     8161 taccttcaat tcgttttgta gttcttctat tgaacaccaa cgttctgttt caagcagtaa
     8221 atgaatcatt ttaattttct tttgagaaac ttttgttaaa aaagtctgca tattttctcc
     8281 tcctgtatct actttactat actacataca agaaaaaaaa cggatacaaa tatcatattg
     8341 tcacaccgtc aatagtgcat aaacctgtta ttatttctct ttttttccta agtattctat
     8401 tgatatgaat catttaatcg tttattatat aaatgtcaat aatctaataa taaaataacg
     8461 tacatatttt attattgcat acttaataac agaactatag caaaaaatcc cgtgtatcaa
     8521 aacgggatta gattaagttt cctcaatcac tattcataac acaattatta taacaagtaa
     8581 gtaaacaatt gaaaaggaca tttattgaaa acggttgttt tttacttaag gaggaaattt
     8641 agtgaaaaaa ttagttgcat tgtttttggt cgcaggaagt ttgttgagtg ggtgtggtgc
     8701 tactacgtct actactcaaa gcacaagttc agctcaggct tccagcagca gtaccacgat
     8761 ctccgaaagt acggcagatt catcgaaaga acaagaacaa attgaaaaga aaaaagaaga
     8821 agagaaacaa aaagcggaaa aagctgcact agaaaaaaaa gtgacagaag cggaatctgc
     8881 tttgtcacaa gctgaagcaa atcctacaaa agaaaactat gcaactgctt cagcagctat
     8941 ccaagcgcta cctgaaaaca aacaagagtt gacaagccgt cttgctgcaa ttgattccac
     9001 aatcaaagca aaagaagcag aagaagaacg gaaaaagcaa gaagaaattg cacgacaaca
     9061 agcagaacaa gcgcaacaag aagcaatcgc acagcaacaa gcagaacagg cgcgacagca
     9121 agcagcagct gaacaggcct ctcagcaggc acaagccgca gctcctgagc aaaataatga
     9181 acaaactgtc tatgtcacac cgaccggttc aaaataccat acacataaat gtggaaatgg
     9241 tacgtatacc cctgccactt taagcgaagc tcaagctcgt ggtttaaccc cttgctccaa
     9301 atgttatgga aactaattca aaagttatac tataataata aaattcttct gaaacataaa
     9361 aaaatagaag ctggcgttcc agatagtaaa ccaaaaatag cagtttacac gtccatttcg
     9421 tcagcttctc tcttttttca tttagtctgt tccctctgct tttttggaca tcttcattca
     9481 ttctttctca atcaaatagt acacctcttt ttcttttcgc agtttatatc ttcctttttt
     9541 caatttcatt gaaaactgtt tcgaatgttt atgttttaca tggattttaa gcaggagctc
     9601 ctcatgttgc aagcgatagt tcctgccttt taaatcagaa aatctcacta gtccgtattc
     9661 atcagaagct ttcacaaatg gaactttttc tctagtaata tagtgtttgc cgttgagcgt
     9721 ttttacagaa aagaccattc cttcgattgg tcgtccgttt gtgttcttta cataaaaaga
     9781 taaaggatag tttcgcttag cgatggccaa agaatctaaa taagcgatga gtagccaaac
     9841 taagccgatt gatccaagta gtataccgat aaaccaaata aaaaatttcc attttttcca
     9901 ttgatcaatt ccttggatcg ctttttttgc ctcttttgct tgataaggaa tacgatgccc
     9961 cgtcacaagc agtcgatgtg tattgaccat ataaggagta caggtaagca aagtcacgag
    10021 atctctgcct ttttggatac ccaaagaatc tacttcatca ggtaagacca cttggatttt
    10081 ttctacttga tacgccaatg tctttccatt gatctggatg aaaaactggt ctcctttttt
    10141 caacttaggc aaatctgtaa ataattttgc ttctgggagt ccacgatgcc cagaaagaac
    10201 ggcatgggtg tttttcccgc cagtcggata agaactttct tccaataatg acgttccttt
    10261 ttgcaaaaat atttccgttg tctgatcgaa tataggtaag ctgacattga tttttgggat
    10321 ttcgatgact ccaagcatat gttccataaa aaaggcatta ctaggtaaat cttttaatgc
    10381 ttttgcatca actgcttggt tgaaactgcc tatccctggt gatacgtttt gctctgccaa
    10441 ctgttggttt ttctttgtca ttttttcctg ctgttttttt atttcctctg aattcttatt
    10501 cgatgcttgc ttttgataat ggatgatcat ttgctgggca agaaaatcat tcagtgaatc
    10561 ttctacaaaa ggataagaaa agatcccgat cccaacgact aaaatgattg ccatgaagat
    10621 gtccagtatt cttattctgc ttttattcat catgtccacc tattttctaa tatgtatatg
    10681 aagag
//
LOCUS       DAMYXM010000077        10661 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.080, whole genome shotgun sequence.
ACCESSION   DAMYXM010000077 DAMYXM010000000
VERSION     DAMYXM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.080"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            333..2279
                     /locus_tag="PNS21_002334"
     CDS             333..2279
                     /locus_tag="PNS21_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI5160022.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            2282..2737
                     /locus_tag="PNS21_002335"
     CDS             2282..2737
                     /locus_tag="PNS21_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI5160023.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            2751..4079
                     /locus_tag="PNS21_002336"
     CDS             2751..4079
                     /locus_tag="PNS21_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDI5160024.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            4113..4397
                     /locus_tag="PNS21_002337"
     CDS             4113..4397
                     /locus_tag="PNS21_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDI5160025.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            4399..4914
                     /locus_tag="PNS21_002338"
     CDS             4399..4914
                     /locus_tag="PNS21_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HDI5160026.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            4930..5592
                     /gene="rpe"
                     /locus_tag="PNS21_002339"
     CDS             4930..5592
                     /gene="rpe"
                     /locus_tag="PNS21_002339"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDI5160027.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            5599..6171
                     /locus_tag="PNS21_002340"
     CDS             5599..6171
                     /locus_tag="PNS21_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDI5160028.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(6358..7878)
                     /locus_tag="PNS21_002341"
     CDS             complement(6358..7878)
                     /locus_tag="PNS21_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDI5160029.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(8121..8306)
                     /locus_tag="PNS21_002342"
     CDS             complement(8121..8306)
                     /locus_tag="PNS21_002342"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDI5160030.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(8290..8472)
                     /locus_tag="PNS21_002343"
     CDS             complement(8290..8472)
                     /locus_tag="PNS21_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160031.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            8713..8931
                     /locus_tag="PNS21_002344"
                     /pseudo
     CDS             8713..8931
                     /locus_tag="PNS21_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            9426..10025
                     /locus_tag="PNS21_002345"
     CDS             9426..10025
                     /locus_tag="PNS21_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI5160032.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            10089..>10661
                     /locus_tag="PNS21_002346"
     CDS             10089..>10661
                     /locus_tag="PNS21_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage abortive infection AbiH family
                     protein"
                     /protein_id="HDI5160033.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT         3525 a         1674 c         1990 g         3472 t
ORIGIN      
        1 taggactcaa ttgttcatag atgagcattt gtaattaagt tatattataa taaaaaaata
       61 cgaattttag tgacgccatt atattgtttt tatttttcaa ttgtaatatt tcatattact
      121 tccataataa ggagtatttt ctattggaat atcttagctc ttttaaggat ttctcctttt
      181 ttgtttctca ttttttaaga ttatttggat ttcctttaca acaaacttcc acaagtatta
      241 ttgacataga tgtaattgag ggaagggggt tccagtgcta gaataaataa tgaaaagagg
      301 taacaaatgc tagcgttaag aggtgaacaa gtttgattga aagacatgta aatttattaa
      361 aattattaag tcgacaagaa gactttaaac ccgcttcttt ttttagtgat aaattggcag
      421 tgtcgaccaa gaccatttat caagatattg aattgttgga agattatgta gttaattacc
      481 aagtaacaat tgagcgatta ccttctttgg gtatcaaact cataggtgat aggaaaaacg
      541 ttgaacattt gatttcatcg gtagaaaatt atcttgaaaa agatatttat tctccagaaa
      601 aaagacgtgt cgagatcatt aaagaagttt tgtttggtga taagcctgtt tctttaactc
      661 atttgtctca aagttttatg gttagtaaaa catcgttata caatgacctg agaattatca
      721 ataaaattat tggcagtgga gaagttaagt tgcagtcatc tgcagaagga attagtttta
      781 ctggtaatga acaagcaatt caacgagcaa tcaagcaact tgtattctat tatgcgaatg
      841 acatggaatc aaaaacattt tttcaagttt taaatatgtt gtttgattca aaagttatta
      901 gtgtcattca tgatttgcta ttcaatgatt acagtgaact gactgaaaag gtttctgatt
      961 actatgttcg gtcattgatt tctactttag ttatccaatg taaccgttta ctgaaaggtc
     1021 atcatttaac tgttgaagaa gaattcctct tcaatagcat tcgctatatg gaaacgtaca
     1081 ttgtggcaaa tggtttaatt gaagaattga aggcaaaatt gaatcttcca ttttatgacg
     1141 cagataaaga atatctatgt cgtcaactat ttgcacatcg tgtgacaaat aatttaaaaa
     1201 ccagcaatga agagtacgct atgagcgttc ataaattgat tgaaagaatg agtgagattg
     1261 aaaaaattga tttgactcaa aatgatcgat tatatcaatc cttattgtat catgtgccag
     1321 caatggtcct tcgtttaaaa aaaggtatta aaattcaaaa tccgttatta gaaaatatca
     1381 aatctcaata ttcagaactc tttagcatag tatggtacgc catgtccatc cttgaagaag
     1441 aatttgatgt gactttaacg gacgatgaag tttccttaat tttgattcat tttcagattg
     1501 ctattgaaga tcagtccaaa gcaaataata tcatcattat ttgccaatac ggtatttctt
     1561 ctgcgcaatt aatttacaat aaagttcgac ggtttttacc agcaaaagat aatgtagaaa
     1621 tatcaacttt agataagttt catttagctg aaattgggga agttgatttg attatttcct
     1681 ctctttcctt tgatgcaggt ggtataccct tagtaagggt atctccattg gtaagtaaaa
     1741 cagattatat gaatattatg gaagcttata ctaaatattt aatagaagaa ccttcgcgat
     1801 ttattcaaaa tgaactgatt cctaaagcgg ttaaagttcc caccatatct aaatttgttc
     1861 aatcatcttt agtaaaaaca ggagtacagg tggaaagcaa agaagaatgt ttggatttga
     1921 tgatttccga actagaagcc aattcattag ttactgaaga gtttcgtgct tctatcttct
     1981 ctagagagaa aatggggaat actaatttag aaagtggtgt tgctttaccg catgcgaatc
     2041 catcaatggt cattgaatcc aacattttta ttgtagcatt aaaaaatgcg attaattggg
     2101 gcgatcgaaa agtcaaatta gtcattatgg ttagtttagc tgaagaagat gtatctgaga
     2161 ttagaggagt atatgaagaa ctttaccaat ttattgggca aaaagaaatg gtcgatcgct
     2221 tgttacaagt tcatgatagc gaaaaattac taagaatttt tcaacaaaaa ggagaataaa
     2281 aatgtatcta gataaagaat tatgtttatt tcaattagaa gtgaaagatc aaaaagaatt
     2341 gtttcaagta atgtctgaac aattaaaaaa cgctggttgc gtgaaagata gtttcttaga
     2401 aggtatcacc aaccgtgaac aagaatttcc aactggatta gaagtaaatc aaattgggtt
     2461 tgctatccca catacagatt ccgcacatgt aaatagctct caaatctgtt ttgcctcatt
     2521 aaaggaacca ctggtatttt cagatatgac agataaatct catgaaatac ctgtacgttt
     2581 agtctttatg ttggcaatgt cacaacctca tgaacaaatt gatacactac aaaacttagt
     2641 tagcctattt caaaatgaag aaaaagtaaa tgaattactt gcatgtacca cgaaagaagc
     2701 atttattgag ttagtacatt ctgcaggagt ttattaggag gaatagagag atggatattg
     2761 tattaaatgc ctttgaatgg tttattggat tagggtcaaa cgtcttttta ccactgatta
     2821 tttttgtaat cgggatatta tttggattaa aacctggtaa agcatttgtt tccggcgtta
     2881 ctgttggtat cggttctatt ggtttaggat tagttttaag tttattatca gatggtttag
     2941 gaacagcaat tcaacaaatg ggggaacaat acggtggtgg cctgaatatc ctagatattg
     3001 gcgtaggtgt tggtggacct ttggcttact ctacatcatt gggaatttta atgattccaa
     3061 tttcattaat tcttaacttc atatttgtgg ttttgggttg gacaaaaaca ttgaatgttg
     3121 atatttggaa cttttggttc gcaattttcc taggtatgat tactcaatct gttacgggaa
     3181 acttttggtt tggcttaatt ggcgcgattg tcgcagtaat tttacaatgg ttcttagcag
     3241 atcgtacaca aaaaactgtt agcgaatttt tcggctatcc aggaatctct atcacacata
     3301 tgatgggatt atcaggagta ctatttgcca aaccagtaaa ttgggttttt gatcgtattc
     3361 ctggatttaa taaaatagat gcagatgcag aaacattaac aaaacgtttc ggtgttttag
     3421 gtgacacagt tgttattggc ctaattatcg gtattgtagt gggtattatg gcaggctatg
     3481 acgtagcagg aattggaaac ttaggaatta tgactgcggc agtgatgaag attatgccta
     3541 aaatggtagc aatgtttatg gaaggtttaa tgccaattgc agaagcggcg aaagaattta
     3601 cgaataaaaa gttaaatggt cgccaagtaa acattggaat ggatgcagcg ttaactgtgg
     3661 gtcatccaac tattatgtca acttctctat tgcttgtacc aatctcatta ttgctagcac
     3721 taattcttcc aggaaataaa gtattacctt ttggagattt agcattcttt gctttcgcca
     3781 tctgtttaat gattccttat tttaaaggaa atattgtgag atcattagta ggatgttcgt
     3841 tgtatgttgt tacaacatta tatatggcaa catggttggc accaattatc actgatgtgt
     3901 ttacattagc aagtttcgat gtggggacag caggattagt aactatgttg ttatgtggat
     3961 tatggccagt agggttgttt gttttattag catcttactt tggtgtaatt ggaataggtg
     4021 gattaggagt tgttttagta ggtgcttcta tctacatgaa caaagtgaag aaagcttaat
     4081 cataaaatta aaataagaaa gaagagatca atatgaaaaa attattagtt atttgtggag
     4141 caggtcatgc tacttctaca attgcagtag caaaattatc aaaatggttg gaagaagaag
     4201 gattaacaga tcaagtgaaa atttatcaat ctaaaattgc tgatgagcta aataaaattg
     4261 atgaatatga tgctgtagta agtacaacaa ttgtccctga tgtaattaaa gctaaagtaa
     4321 cgaatggctt gggcttatta acaggtattg gtacagatgc catttatgca gatgtaaaag
     4381 aaagattagg tgtttaatat gcgttttcat catgacactt tgactattga aacagtttca
     4441 gggcgtcctt cgtatcatga tattcgaaaa gaacttttag atacagttga tacagcaggt
     4501 attgagaatg gaatgttagt aatttcgagc ccgcatacaa cttgctcact cttttttgaa
     4561 gaaacgatgc atgacacaaa cttttttggt gatgattatt tacatgtaga catcaacaat
     4621 gtaatggaaa cgattattcc aaaaatgacg agtgaaaatc aatacaacag tccggggcca
     4681 aaacacattg attttggaac aagattatct gatcccaatt accctgcaga aaaatgggtc
     4741 atgctaaata ccgatgcaca tttacgttca tctatttatg gaaccaactc aatgacttta
     4801 attgttcaag aaggtaaact tttattagga gaaatgggcc gtgtctattt tgtcgattgg
     4861 gatcaactaa gagagcgaac tagaacaatc aatctcatga ttatgggaga ataaacgggg
     4921 gaatagagaa tgacaattgc tccatcattg ttaaatgcac ctatcgcaga agttaaagag
     4981 ttatttgaaa tcttaaaaga aaagaatgtt gattatttgc atattgatgt aatggatggc
     5041 tgttttgtac cagatatggc ttttggacca agtgttatta aagatattcg tccactaaca
     5101 gacgttgtgt ttgatgttca tttgatgatt gatacaccag aaaaaattgt cacaagattt
     5161 gtagaagcag gagcagatat tgttaccttc cacattgaag ctaccaaaca cgcaatgaag
     5221 gtcattcagt tagtcaagga agcaggagca aaagtaggga ttacttttaa tcctgcaaca
     5281 ccagtcagtt ttattgaacc agtattatct ttagtcgatc aagtattagt aatgacaatc
     5341 aatccaggaa cagagaataa gcattttatt caagagacag tagttaaaat cgaacagtta
     5401 gatgtaatac gcaaacaaaa tgattatacg tatgatattg aagtagatgg caagattgat
     5461 aatcaaacga ttaaagtttg ttctaaagca ggggcggata tttttgtatc aggcggtttc
     5521 atcttcaatg ggaaaaatac cccgcaagaa caaattgata aactaaatca atcattagag
     5581 gaagtgaagt aaaagatcat gaccaataaa gaccaaaata aaaactattt agaagcgatt
     5641 atcttagaat tacaaaataa ctcaaaacgt attaattctg tagagttaga acaatttagt
     5701 gagaaaattg ttcaagctaa acacatcttt ctagctggtg caggacgctc tggaattgcc
     5761 atgcaagcat ttacgaatcg cttaatgcat ttaggttttt cagtcagcct tgtcggtgaa
     5821 attagttcac cacattcgca acctggagat ttattaatta tctgttctgg ctcaggtgaa
     5881 acaggtagtt taaaaaattt agcggccaaa gcaaatcaaa gtggtgtaag cattgcttta
     5941 gttaccatga aaaaagactc gtcgattggt caattagcag attctgttct tgtattacca
     6001 ggtattacca aagatgaaaa tcaacgagaa gaagatactt tttcacaacc tatgggttca
     6061 agttttgaac aattagcatt tctcaccttt gatagtttag tgttatcttt aatggataaa
     6121 acaggtgaaa gctcagaaac gatgtttgct cgacatgcag attttgagta gtagatttta
     6181 aagataaaaa accattctct aaatgctaat tattcagcaa atatgagaat ggttttttta
     6241 tttgattaga cataacttaa taattcagta gttttgagcg taagcgtgtc atagaatggt
     6301 acctaaacac aaatagcccg atttgataca ctcaaatcag gttatttgtg gcttatatta
     6361 tttacagatt tcttgtatct gctgattcca tggcaaataa gcgcgtagcg cttcaggtgt
     6421 catcgaagac agattgggaa tctctgtaaa aagatgattt aaatatttgc ggatgtttaa
     6481 cccattcgca acagctgtct ggataatact caatataatt ccatttgatc tagcgccttt
     6541 taagctttta gaaaatagcc agttttttcg accgatcact aactctttga tggcacgctc
     6601 tgctcggtta tttgaaagtt ccaagcgtcc atcaagcaac acaatctgaa gtccgtcttt
     6661 atgattgact gcgtattgaa ccgcctttcc aagctgcgat tttggcaaat aagcaaaact
     6721 ctcacaccaa tgaaaaaatt cagtcatcac aggctgcaac gtttccttcc tcgctacaaa
     6781 cctttcttct gcgctaagct tattaaaggt ccgttcgatt cgaaacatct tgtcacagaa
     6841 gtctaatcct attttggcat gactttttgg ggacggttgt ttacccaatg cttcaaaaaa
     6901 ctttctgcga acatgcgccc aacaataaac attgtttacc ggcagctttc cataggccgc
     6961 gtaaccatca gtatgcaaga aaccgctaaa tgattctaaa aaactttttg gctcatcaac
     7021 ggaacgcgaa tttgcgtgct cgtagagcac aacttgtttt tcttcgcttt tacctgaagc
     7081 atataaccag taaaaggtct tttctttatg agtattcaat acccgatagc ttgtctcatc
     7141 cgcgtgtaag acctcctgat tgcttaattc ttgatgaagg caattgtaga ttggctctaa
     7201 aaccatttca cttaccttga tatgccaatt agtgattttt ttacgatcaa gagggaaacc
     7261 aagtgcatgc cattcttttt cttgtcgata tgccggcacc tttttaacaa atttttccca
     7321 aatactctgt gtcacaattg aagaggaacc tagactatta ttgatcaacg gtttaggaac
     7381 aggcgctttt ttgatttttg tttctcctaa tgcttcacat tctctgcaat gataggcatg
     7441 ttggtagatg acttgttttt ctatatgggc aggaacatac aatggctctt ctctaagcct
     7501 tgtcgcaccg atatcatgta aagtatgacc acagtcttca cacaaacaat cggcaccttt
     7561 taattcataa tgaataggaa cttcaggcag accttctaat gaaagtgctt tgagtccctt
     7621 gcgttttctg cgtttaaaag aagataccgt aatgggttcc tggctttctt gtccagtgtg
     7681 ctctggttca ttaaaagttg agtcgtctaa taaatcgatt tgcccatcta cgggtggtgt
     7741 ttcttttttt cgtccaaaca agcgtcgatt catttcagca atttgttctc taagaagagt
     7801 attttcctgt tgctggattt caagcaactg tgttaattgc ttgatttgta caagaagttc
     7861 agattccatt tttgtcattt ttacaccacc attctagcga ttttcttaca tctattatac
     7921 cattttatga caacgaaaaa cagacaaaat ccttgattta acaaggtttt cgtctaatat
     7981 atcgcacctt ttttcgtttc ttttagcgta catttttctt cgattgaaag gccagtcagt
     8041 aagacttctt ttctggaacg cggccattga aggtacccac tttcaaatct tttgtacatt
     8101 aatacaaatc cattttgatc ctagtaaaga gccttgaagc gatctttctt cgtaccgcaa
     8161 aataagaaaa gcgcttgatc gtaaacatca aggtcatatt cttctataac gaaagctgct
     8221 agtccatcaa ttccttttcg aagatctgtt tttccacaaa cgataaaaat atgtttcaat
     8281 gcagttgcat caatgagcat tggcattcaa ctcttttaac aaggtataca aaagatattt
     8341 atccattcca ttataaataa atatttcctt atcttccgat ttgattctgc aagctagttt
     8401 tggcttggtg tcaatttgcg atttttgagc cgcacttgaa aattgaactg gtacaatatc
     8461 ttttttattc attcgttttt ctccttctat atttgatagt ttaattatag aagaaagata
     8521 atcggcgtac gagatactat cctatgacac gcttacattt aatttaatat tttgcctcaa
     8581 attagccaca ctttttatag aactaagagg aaaaatgaag ttttcttcct attaatatag
     8641 ttgtttgaaa tcttaaatat agcgttttac gatgtaatgg gaagtatggt gaataaatca
     8701 actatgttcg tacgaagcat ttactcaact aatttaatcg aatctttcaa taaacaaatc
     8761 aagaaataca gccaccgcaa ggaacagttc caaaatgaag agtcgatgga acgtttctta
     8821 gtctcgtctt ttgatactta caaccaaaaa ttcctaggtc gcagtcataa aggtttccaa
     8881 caggccgaag gcgagctcga acaaatgctg agtcaaccga tggagaatta gaaacaatct
     8941 acagtaggga agaaccattg acacaaaatt attgacgctc ccaactaaaa gtgtcaaaat
     9001 aatgatgaaa tcatttagtt cttataaatt agaaaagaga aaataaatat tccatatcta
     9061 ctttattgga tccgtaacat gactaaattc tgaatacttt ttaaagctta tcatttggta
     9121 aaactgaata taagttaatt atatagactg aaaattttga attttcagaa tagaataact
     9181 taaaagtact gacaaaattt tttaaagggg caatattaat gttctgagat aatcaaatcc
     9241 taccgaaaat atcaaaaatc caatgtgaca caatcagatt gtgtctcatt cgagggaatc
     9301 acacaatccc acgtggctct aggcagaatt gaatttttgc ttggggcttt ttccttatat
     9361 tgattttgaa tgagacttat tatatgttat aatgcaatta agtatcattg gagagggcga
     9421 agaaaatgaa gacggtagaa cctattcgag acaaaaaaaa gatcgatgcg atgaaggcaa
     9481 tcttggcttc gggaaaatat ggccaacgaa atttggttct tttttcgatt gggatcaaca
     9541 ccgcctatcg catttctgat ttgcgtcaat tgaagctggc cgacgtgctg gaaatttcac
     9601 gcggtcgcgt ggtggtcaaa gatcgattag cgatgaagga acaaaaaaca gctaaacata
     9661 attcggtcta tatttccaac aaattgcgaa aaacgatttt ggagtacgtt caacaggaat
     9721 ttcctgatca actccttcag caagattttg acggttattt gttccctagt cgaaaaggga
     9781 cgaatgaacc gctcaaccgc caaagtctgt ggcgaatcat tcatgatgct ggcacggcaa
     9841 tcggattgaa agacattggc ccacattcca tgcgaaaaac gttcggttac tttctctata
     9901 aacaggggac caaaacagaa atcattcagt ccttgttgaa ccattcttct cagcgcgaaa
     9961 cattgcggta tattggtatt acacaagaag acaaagatac agctgtaaaa agcttagatt
    10021 tataatataa aagttaagtt taaacatgga tagttttaaa ttagtagatt gagtgttggg
    10081 aggaaagaat gagtacatta tatattatag gaaacggttt tgatttaagt catggtttaa
    10141 aaactagata cactgatttt tcaaaatggg ttgaaaaaag tcatagtgaa atattcgata
    10201 aaatagacac aatgataaat gaaaataatg aggtgagaag tacagctgat gataagatag
    10261 gttggtttaa ttttgaaagt agtttgcatg agttaattca aagtccagtc tttgaagatt
    10321 atgtagaaga gtctaaggat tatctcatag gttatggaca tgaagattgg tcagattctg
    10381 cccatcatga ttttcaaaat atggtttatg attatgttgt ttctctagaa gaattacaac
    10441 aacttttcaa ggaatggata cgcaaagaaa tacagccgac aatagaacat aaaattccaa
    10501 aataccaatt ttcgtccgat gtactattct ttacattcaa ttatacagat gttttggaaa
    10561 ttctatacaa gcaaccgaaa gaaaacatat atcatattca tggcagttat gacgatataa
    10621 taatcggcca ctccgagaat tttgacaact ttataggaga t
//
LOCUS       DAMYXM010000078        10603 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.081, whole genome shotgun sequence.
ACCESSION   DAMYXM010000078 DAMYXM010000000
VERSION     DAMYXM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.081"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            23..646
                     /locus_tag="PNS21_002347"
     CDS             23..646
                     /locus_tag="PNS21_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160034.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1095..2144
                     /locus_tag="PNS21_002348"
     CDS             1095..2144
                     /locus_tag="PNS21_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI5160035.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2141..3022
                     /locus_tag="PNS21_002349"
     CDS             2141..3022
                     /locus_tag="PNS21_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5160036.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3025..3795
                     /locus_tag="PNS21_002350"
     CDS             3025..3795
                     /locus_tag="PNS21_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI5160037.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4074..4928
                     /gene="aroE"
                     /locus_tag="PNS21_002351"
     CDS             4074..4928
                     /gene="aroE"
                     /locus_tag="PNS21_002351"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDI5160038.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5106..6269
                     /locus_tag="PNS21_002352"
     CDS             5106..6269
                     /locus_tag="PNS21_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDI5160039.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6386..6796
                     /locus_tag="PNS21_002353"
     CDS             6386..6796
                     /locus_tag="PNS21_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI5160040.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6970..9198
                     /gene="topB"
                     /locus_tag="PNS21_002354"
     CDS             6970..9198
                     /gene="topB"
                     /locus_tag="PNS21_002354"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDI5160041.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9369..9653
                     /locus_tag="PNS21_002355"
     CDS             9369..9653
                     /locus_tag="PNS21_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160042.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9813..10049
                     /locus_tag="PNS21_002356"
     CDS             9813..10049
                     /locus_tag="PNS21_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDI5160043.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10046..10444
                     /locus_tag="PNS21_002357"
     CDS             10046..10444
                     /locus_tag="PNS21_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDI5160044.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3703 a         1770 c         2242 g         2888 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aattggcgca gctggcgtag ataagttgta ttaatggtga gactttccca
      901 tagatacaac tttttagagt gcatttctaa agcttgtatc tatgctagac atgaacattg
      961 actgccgcat agattagaat ctatgcggtt ttttgtgtaa agacaatgac cgctttggcc
     1021 attgtctttt tttgtagact ggatacttgc ctgattataa taaataaaaa gattgacttt
     1081 gcaggaggaa aaaaatgaaa aacaaacgat taataacagt tgtagtaatt attttagttt
     1141 atttagcagg tgcatttatt tatggaaaag cctcaggcga aaaagaaagt acttcgacga
     1201 accagacaaa aaaaacaaca gttgttgggg tattgcaata cgtgagccat ccggcattag
     1261 atgagattta tcgaggaatc aaagatggtc tgaaagaaga aggattaaaa gaaggagaaa
     1321 atcttgagat caaatttcaa aatggacaag ccgatcagag caagctagcc acaatgagtc
     1381 aacagctagt acaatctgat ccagatgcct tgattggaat cgcaacacct gccgctcaat
     1441 cattagcaaa cacgacttca tcgatcccag ttgtgttagg tgctgtaacg gatcctgtgg
     1501 gggctggatt agtcgcttct ttagagaaac ctggtggtaa tgtaacaggt gtttctgatc
     1561 agccgcctgt tacttcacaa atcaagcttg cttctgattt gttgccaaat gcaaaaaaag
     1621 tcggaatctt gtatgcttct tcagaggata attcaaaata ccaagtagca caagcagaag
     1681 aagctgcaaa aaaagaaggg ttagaatcag taaaatatgc ggtgccttcc actaatgaga
     1741 ttgcacaaac ggttcaagtg atgagccgac aagtcgattt tatctatgtt ccattagata
     1801 ataccattgc caatgcgatg caaacagtgg taaaagaagc aaacaaagca aatatccccg
     1861 tcattccatc tgtggataca atggtggaac aaggtggact agcaacaatc gggatcaatc
     1921 aatatcaatt agggcttcaa agcggtaaga tggcggcaga acttgcgagt ggaaaagaaa
     1981 aaccggaaac gacgcctgtt tatacatttg atcaagggga tacagtgatc aatcaatctc
     2041 aagctgatcg cttaggaatc attgttcccc aatcgatgaa ggaaaaagcc aaaatcatta
     2101 cagatgactc gcagcaagaa acatctaagg aggatgacaa atgatcgtat cagctatttc
     2161 acaaggcatg ctttgggcaa tattaggatt agggattttc atgacttatc ggatcttaga
     2221 ctttcctgat atgacgacag aagggtcctt cccattaggt gctgctgtct gtgtgacagc
     2281 tattactcat ggggtagcgc cgattgtagc tacattatta ggcgtaggtg ctggcatgat
     2341 agcaggactc gttacggggt tactttatac aaaaggaaag ataccggtta ttttagcagg
     2401 gattttagtg atgtcaggat tgaactcagt tattttgttt gtgatgcgaa caccaaatct
     2461 ttctttattg aatcatccga ttctacaaga cagcttccat cgctttgggt tacctgatta
     2521 ctttgacaca gtccttcttg gcttcatctc tttgggcatc ttgattgctg tactgctatt
     2581 cttcttcaat acagatttgg gtcaaggcta tattgcgaca ggagacaacg aaacgatggc
     2641 tcggtcgtta gggataaaaa ccgatcgtat gaaaatttta ggtttaactt tgtcgaacgg
     2701 tgtcattgca ttatccggtg cgctgattgc ccagaacgac ggatatgcag atgtaaataa
     2761 agggattggc gtaatcgtca tcggtcttgc ttccatcatc atcggtgaag tgatcttcca
     2821 agaattgacg ctagcagaaa gattgattgc tattgttgtc ggcagtatca tttatcagct
     2881 attgatttta gtagtaatca aactagggtt tgacacgact tatctgaagt tgttctccgc
     2941 tatcatctta gctatttgtc tgatgatccc gcaattgaag aaagcactga acttaaaaac
     3001 gggttttgag aaggaggcat agtaatgact gatatattga caatcaaaaa tggcagaaaa
     3061 gtcgtaaaca atggaatgaa tgaagagaaa gtactattgg atgaaattaa ccttacaatc
     3121 agagaagggg acttcatcac tgtccttggc gggaatggtg ctggaaaaag cacactgttc
     3181 aatacgatcg ctggtacgct tcaactcagt caaggatcga tctcttttaa agatcagctg
     3241 atcacaaaag attcagaaga aaaaagagca cgattccttt ctcgagtttt ccaagacccc
     3301 aagatgggaa cggctcctcg gatgacagta gcagaaaatt tgctacttgc acaaaaaaga
     3361 ggtcaaaaac ggaaattccg tttacgtaac ttgaaaaatc aaaaagaaga ttttttcaga
     3421 ctatgtcaag aagtcggtaa tggcctagag caacacttgg atactccagc gggtgaatta
     3481 tctggtggtc aacgccaagc attaagcttg ctgatggcga cgatcacagc tcctgagtta
     3541 ctattattag atgaacatac tgcagcttta gatcctaaaa cagctaaagc attaatgcga
     3601 ttaacaaatc agcggatcaa tgaacaaaaa ttgacttgtc tcatgatcac gcatcgaatg
     3661 gaagacgcct tgaaatacgg aaatcgtctg atcgtactcc aaaaagggaa aatcgttcaa
     3721 gatatttctg aaaaagaaaa gcaacatttg acaatgggcg accttttgca gttctttgaa
     3781 gaagtagtgg attgatttcg acagaaaaat tcctttcagt agaaggactt gaaaactcat
     3841 tgacatcttc ctttgattca tcctatactt aaaacaataa acaattaaac atatttgggt
     3901 gttttgtagg atcacgtagt caatgatttt aacaattaaa tagaatattc tttgctctga
     3961 caagtaaacg atgagaaaag aatttgctac tttgccacct agacgtttcg tactttttcg
     4021 tatcaaacgc taagtggctt tttttattga agtaagaaca aaggaggaat aagatgattt
     4081 ctggacaaac caaattagct gggctctttg ctgatccggc ttctcacagt ttatccccat
     4141 tgatgcacaa tactgctttt gaagcaaaca acatcgatgc ggtgtattta gctttccaag
     4201 taaatccaat gaatttgaag caagcagtcg aaagtattcg aacttttgat atgttaggtg
     4261 taaatctttc gatgccgaat aaaacagcgg ttattcctta tctggatgag gtaagccagg
     4321 aagcgcaatt gatcggagca gtgaatacga tcgtgcacga aaagggtcgt ttgattggct
     4381 ataatactga tggtgcgggc ttcatgcaat ccgtgcaaga agctgggatt tcgatcaaaa
     4441 agaaaaaaat gactctactg ggagctggag gtgcagcaaa ggcagtcact gttcaagcag
     4501 cgttagacgg cgctgaggaa atcgtagttt ataaacgaaa caaccaaaca ttcgctcaag
     4561 taaaagaaac attcgaacaa ctcagctatc gaacttcttg taagataaaa gttcgtgact
     4621 ttgccgatac gcatcaatta agaaaagact tgaaagaaag ctgtcttcta gtcaatgcaa
     4681 cagatatcgg gatgggaaag aaaatcaatc agtcaccgct cttagatata agcatgatgc
     4741 atgagaatct agcggttttt gatttgattt atgcacctag agaaacacgt ttattaaaag
     4801 aagcaaaaaa agtaggagca aaaggataca atggacttgg catgttgatc taccaaggag
     4861 caatcgcgtt tgaattatgg acaaaggaaa agatgccgat ccatgtgatt gaaaagctgt
     4921 ttgattaaaa aaggataata gataaagatc ttttattctc gcataaacgg tctcaagaaa
     4981 agtaatcctt tatgtatcga agcatttatt tcgttatttt ttattaaatt atcaaaaaat
     5041 gataggcttt tgtcgattgg ttatcgtatc ctttatttat cagtaagaaa cgaggaggaa
     5101 ccacaatgag tcggatggat cgctataagg acatccataa gaaggcgaaa cctctgaagc
     5161 gggatacggg gagtattttt gctagaagag aacgaaaacg aacgcgtatg gaaaatgagg
     5221 aagatgctac ccgtatttat gaaagagagc agcattcatt tgaacatttg aaccaatacg
     5281 agcaacagat atacagagat gcaccaagag cagaagaaca acaaaaaaat aaaaaatttt
     5341 tcaatcggca aaagaagaac aaaccgttaa aacctcgaag aaagcgatcg tggaaaaaaa
     5401 tcattctatc gattttgctt gttttgatcc tttattcagg tatctcattt tttattggta
     5461 aatatgtagc tgaacatgac acaagcttag aggcagcgac ttcagaaacc tttcatgggc
     5521 aaaaagcgac gaatggtgcg cataatattt tgatccttgg aagtgataca cgcggagaag
     5581 atgccgggcg agccgatacg atcatggtct tacagttgga tggtccttcc cataagccga
     5641 aactgatttc ttttatgcgt gacagcttcg ttacgatccc aggggtagga caaaataaga
     5701 tcaattcagc ttatgcatac ggaggagccg atctagtgcg tcaaacttta gtagaaaatt
     5761 ttggaatcga ttgtcaatac tatgcgaaag tcgatttcaa atcgtttgaa aaagtgatcg
     5821 atgccttatt catgaacgga gtgaagatcg atgcagaaaa agatctgaat ctggatggtg
     5881 tagatattaa aaaagggggc cagaagatgg atggacatgt tcttttgcag tacgcacgtt
     5941 tccgaatgga tgaacaagga gactttggcc gtgtcagacg ccaacagcaa gtaatgaacg
     6001 ctattttcag ccaattgaag aatccattga atctgatccg tgcaccttat gcagctggta
     6061 aagctttagg atatacatcc acagatgtca ctgcttggtt cttgacgaaa aacctctttt
     6121 cgattgcacg aggagctggt ggcattgatc gaataagtgt gccagtagac ggttcgtgga
     6181 attatgggca aagtgattat gctggcagta ttcttgaaat tgataatcaa atgaatcaat
     6241 ctgctattac agattttttg aataaatgat cataaaaaag atcaagaaga agctgcgcaa
     6301 ggcttttgtc atgaatcaat caaacgattc actcgtaaaa aacgctttct tatggtagat
     6361 ttaaccaaga gaggagctgt ttacattgga tatctatctg aacttcaaaa atgagtcgcg
     6421 tgaagccatc gctttttatg aaaaagtttt tcagactgaa gcatcaggta tcaccacata
     6481 cggtgaaatg cctggaatag aacaaatgaa cttgccagaa gaagcaaaaa atctaatcac
     6541 gaatgcacgg ctgaacattt atgatacatt agttatgttt gcagatacgc cagaaggaat
     6601 tggtcctgaa ttagttgtgg gaaataatgt ttctttagtc atccagacaa gaaatgaaga
     6661 agaaacgaag cgcctatttg aaagcttagc tgagggtggt aagattgaga tgccactgga
     6721 tgctgttccg tgggctcagt tgtatgggat ggtaactgat cgatttggca tcaactggca
     6781 gattaaccaa gagtgacagc taaagatgaa tattttctgg aaacatcaag cagcaaacgg
     6841 atcatcgttt gtttgcttga tgtttttgtg tctatttcga ctatacggta tctttttttt
     6901 attgccagat gcgaaaatat gttccataat agagaagtcc gcaaaaaatt aagtaacgga
     6961 ggtcagaaga tgaagacggt gatactagct gaaaaacctt cacaagcaaa agcctatgcc
     7021 gaaaccttcc aacagcaaaa gcggcatgaa gggtattatg aaatcagcga tccattgttt
     7081 gctggtgaag taaccatcac ttatggtttt ggacatttgg tagatatggt tgctccagga
     7141 gcttatgaag agcgttgggc aaaatggtct ttagaaaatt tgcctatttt cccagaaacc
     7201 ttccgttacg aagttccaat ggacaaacgt gcacaatttt cgatcgtaaa acgagaattg
     7261 cagtcagccg ataccatcat tattgcaaca gatggtgatc gagaaggtga agccatcgcg
     7321 tggtcgatta ttcgacaagc aaacgcattt tctaaggaaa agaactattt acgtttatgg
     7381 atcaattctc ttgaaaaaga agcaatctat gaaggtttta agaatctcca acctggaaag
     7441 aactattttc caaaatataa agaagcgcaa gcaaggcaga acgctgattg gctgattgga
     7501 atgaatggaa gtccattata tagcttgcta cttcaacgaa aagggattga tggcagtttt
     7561 tctcttggtc gtgtacaaac accgacactc tatatgattt atcagctgca agaagaaatc
     7621 aaacacttca aaaaagaaac gtactttgaa ggagaaggtt taattacgac aaacaaaggg
     7681 cagttttctg gaaaaatcgt accaaaaaaa gcatttaaaa cccaagaaga gttaacgaag
     7741 gaaatagaaa aacttggagc gcatttaggt aagcaaaacg gacggattct tgaagttacg
     7801 aaaaaggaaa agaggttgca tagtccacgt ttattttcgc tatctagttt gcagacaaag
     7861 atgaatcagc taatgaaagc aagtgccaaa gctacacttg atgctgctca aggattgtac
     7921 gaaagcaagt ttctaagcta tccaagaacg gattcctttt acattacaga aaatgagcat
     7981 cagtatctgg ttaaacatct gcaaaattat aaaaaatttt taggaataga agaagtacag
     8041 acaactgaga tcaaagcaaa gaaacgatat gtcgatgcga aaaaagttca agaacatcat
     8101 gcaattattc cgacaaagac gattccgact cctgcacgct ttgcgaagct ttccaaatta
     8161 cagcaagcaa tctatttgca agttttgcgc actactgtgg ctatgtttgc tgcagattat
     8221 atctacgaag agacgaccgt aatgacgggc gtacagcaat taaagctaaa aagcatcgga
     8281 aagatcccat tacagcaagg ttggcaggaa atcttacgca tcaagtcaaa aaagaaagaa
     8341 gctcagactc ttcctcctat cgtagaaaat gaagaagcag aagtagaact gaaaagtatg
     8401 gagaaagaaa cacaacctcc aaaaccctat accgaaggaa cgttgatcac agcaatgaaa
     8461 actgcgggaa aaacgctgga taatgatgaa gcgcaagaaa ttctcaaaga agtagaagga
     8521 atagggacag aagctacacg agccaatatc attgaaaatc ttaagcagaa gcattacatc
     8581 gaagtcaata aaaacgaaat aactgttacg ccaaaaggaa tcactctttg taaggcagta
     8641 gccaatgaac cattactgac gagcgcagaa atgactgcgc gctgggaagg gtacttgaga
     8701 aaaattggta aacaagaggg gacaccatct gtttttttag agaatatcaa aaaattcatc
     8761 ctacatttat tggcagaagt tcctaatcaa atcgacaagg tagacttttc agatgagatt
     8821 agcgggacac gtcagataaa agcgatggag aagaaaaacg atcaattagg aagatgtcca
     8881 aaatgtaaaa agggaatagt catgctttat cctaaaatcg ctacatgctt gaatccggaa
     8941 tgcgacttca aattgtggcc aacagtagct aaaaagaagc tgactaaaac gaatctcaga
     9001 gatttgcttt caaaaggaaa gacaagtaaa gttgtcaaag gttttactgg taaaaaaggg
     9061 aaattcgatg cagttttagt attaaaagaa gatatgacaa tcggtttttc ttttccatgg
     9121 atcgaaaaca gtggaacaaa agcagttgat ggaaacaaag aaacatcaag aaaaaataat
     9181 gaaaacgaag tagagtaaat gaaaacaatg aaatagcgga atatttattc cgaaaagtga
     9241 gaatataaat accaataaaa ctaaaaaata tcagtgaatc aagcaaaaac gttggggtag
     9301 gctgatttta tatagatagc aaggagtttt tttatttgaa aaaagtgcat tcatttttaa
     9361 attatcttat gggagcaatc acgattgtgg ctgtgtacca agcatttcta aagtatcctg
     9421 attacgggac gaagttgttg tcattgacgc acaatcaaac agcatattca ccaacgttga
     9481 ttttatcgac tttgactgca ggagctaaaa gcgctactta tttttttcat aaaacgacaa
     9541 gcatgctaaa gcgaaagaaa cagcgaaaac aattgttggt ggtggtagcg gctatgatca
     9601 ttatttattt tctaccagtt attacacaag tatttcatca atattttggt taagaatacg
     9661 agagtttgtg acaaaagtca tgtcacaaac ttttttcttt gtcatttttt cgtaagaaaa
     9721 agcaaaataa aagtatatac attgattaaa agtatatact tggatataat gtacttacta
     9781 tagaatgaga atggtataga gggtggcgaa atatgaaaaa aatgaaaaag tataaaatta
     9841 gaaaatctgg caatagcgat gtaacaacta tcccaccaga agtaaaagaa ttcatgggtg
     9901 tgcagacagg tgacgcaatc agttatgttt ttcaatctga tggttctgta cgaatgatca
     9961 aagcacaaga agaacctgat attgattcat tagttgattc gatcatgaat caatatgaag
    10021 acgcgttaaa agatttggtt gatttatgag gtatctttcg actaaagaaa ttatcaaaat
    10081 caacgcgaaa gttattttac gtcactcgcc aggcgaaatg atcggtgtaa aagatgcaaa
    10141 tgcattagat atggccgtca aacagccttc acaagcagta ttcaatcaag aactctatcc
    10201 tgaaatctat gaaaaagcgg cgattttagc aattaattta gctaaaaaac atccttttca
    10261 taatggcaat aaacgaactg ctcttgttgc catgttgttg tttcttcaat tgaataattg
    10321 tccagttgct ttttctcgtc aagaagctgt tgattttatc ataatgatta ccactagttc
    10381 attggatttc gattcactaa agtccaagat aaccagttat ttacgacaaa ccgcaataaa
    10441 atgaacgaat taaaaacttt atttctcaat tagatcagca tgtcattaag gaatatgctg
    10501 atttttttat gtttaatcat acaaaatagt tttttcataa aaatatggaa aatatggatt
    10561 aattttgaaa taattgattt aaaaggaata caaaagagtg aat
//
LOCUS       DAMYXM010000079        10574 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.082, whole genome shotgun sequence.
ACCESSION   DAMYXM010000079 DAMYXM010000000
VERSION     DAMYXM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.082"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            12..84
                     /locus_tag="PNS21_002358"
     tRNA            12..84
                     /locus_tag="PNS21_002358"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="PNS21_002359"
     tRNA            88..160
                     /locus_tag="PNS21_002359"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="PNS21_002360"
     tRNA            168..249
                     /locus_tag="PNS21_002360"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="PNS21_002361"
     tRNA            267..339
                     /locus_tag="PNS21_002361"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="PNS21_002362"
     tRNA            351..422
                     /locus_tag="PNS21_002362"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="PNS21_002363"
     tRNA            439..524
                     /locus_tag="PNS21_002363"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="PNS21_002364"
     tRNA            535..608
                     /locus_tag="PNS21_002364"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="PNS21_002365"
     tRNA            618..691
                     /locus_tag="PNS21_002365"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="PNS21_002366"
     tRNA            711..783
                     /locus_tag="PNS21_002366"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="PNS21_002367"
     tRNA            811..884
                     /locus_tag="PNS21_002367"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="PNS21_002368"
     tRNA            898..971
                     /locus_tag="PNS21_002368"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="PNS21_002369"
     tRNA            995..1084
                     /locus_tag="PNS21_002369"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="PNS21_002370"
     tRNA            1097..1170
                     /locus_tag="PNS21_002370"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="PNS21_002371"
     tRNA            1189..1264
                     /locus_tag="PNS21_002371"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="PNS21_002372"
     tRNA            1266..1336
                     /locus_tag="PNS21_002372"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="PNS21_002373"
     tRNA            1350..1423
                     /locus_tag="PNS21_002373"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="PNS21_002374"
     tRNA            1448..1535
                     /locus_tag="PNS21_002374"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="PNS21_002375"
     tRNA            1557..1628
                     /locus_tag="PNS21_002375"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="PNS21_002376"
     tRNA            1651..1740
                     /locus_tag="PNS21_002376"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="PNS21_002377"
     tRNA            1753..1826
                     /locus_tag="PNS21_002377"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="PNS21_002378"
     tRNA            1845..1917
                     /locus_tag="PNS21_002378"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="PNS21_002379"
     tRNA            1921..1993
                     /locus_tag="PNS21_002379"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="PNS21_002380"
     tRNA            1998..2068
                     /locus_tag="PNS21_002380"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="PNS21_002381"
     tRNA            2081..2154
                     /locus_tag="PNS21_002381"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="PNS21_002382"
     tRNA            2166..2237
                     /locus_tag="PNS21_002382"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="PNS21_002383"
     CDS             2503..3696
                     /locus_tag="PNS21_002383"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDI5160045.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="PNS21_002384"
     CDS             3826..5298
                     /locus_tag="PNS21_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5160046.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="PNS21_002385"
     CDS             5381..6169
                     /locus_tag="PNS21_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDI5160047.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="PNS21_002386"
     CDS             complement(6236..6904)
                     /locus_tag="PNS21_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDI5160048.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="PNS21_002387"
     CDS             7019..8731
                     /locus_tag="PNS21_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5160049.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="PNS21_002388"
     CDS             8734..10506
                     /locus_tag="PNS21_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5160050.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         3033 a         1926 c         2453 g         3162 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggca
//
LOCUS       DAMYXM010000080        10130 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.083, whole genome shotgun sequence.
ACCESSION   DAMYXM010000080 DAMYXM010000000
VERSION     DAMYXM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.083"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            119..625
                     /locus_tag="PNS21_002389"
     CDS             119..625
                     /locus_tag="PNS21_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDI5160051.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            642..1541
                     /locus_tag="PNS21_002390"
     CDS             642..1541
                     /locus_tag="PNS21_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDI5160052.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1628..2512
                     /locus_tag="PNS21_002391"
     CDS             1628..2512
                     /locus_tag="PNS21_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDI5160053.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2704..2775)
                     /locus_tag="PNS21_002392"
     tRNA            complement(2704..2775)
                     /locus_tag="PNS21_002392"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2740..2742),aa:Arg,seq:ccg)
     gene            2835..4169
                     /gene="rpoN"
                     /locus_tag="PNS21_002393"
     CDS             2835..4169
                     /gene="rpoN"
                     /locus_tag="PNS21_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDI5160054.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4318..5409
                     /locus_tag="PNS21_002394"
     CDS             4318..5409
                     /locus_tag="PNS21_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HDI5160055.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5450..6451
                     /gene="gap"
                     /locus_tag="PNS21_002395"
     CDS             5450..6451
                     /gene="gap"
                     /locus_tag="PNS21_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDI5160056.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6561..7751
                     /locus_tag="PNS21_002396"
     CDS             6561..7751
                     /locus_tag="PNS21_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDI5160057.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7855..8610
                     /locus_tag="PNS21_002397"
     CDS             7855..8610
                     /locus_tag="PNS21_002397"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDI5160058.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8726..10024
                     /gene="eno"
                     /locus_tag="PNS21_002398"
     CDS             8726..10024
                     /gene="eno"
                     /locus_tag="PNS21_002398"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDI5160059.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3548 a         1868 c         2099 g         2615 t
ORIGIN      
        1 tttgtcacac cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa
       61 attgaaactt ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat
      121 gatttttact ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg
      181 actcatcgaa actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa
      241 agaagaggcc ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa
      301 agaaaaagag atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt
      361 agatagttgg cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc
      421 ccaacagtta cctttagaaa cttctgttgc agagctccat atgattttag ataaaataga
      481 cagtccggaa aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat
      541 tgcgactatc ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa
      601 tgctttacgt caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa
      661 atttaaaaaa actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg
      721 agatccgcca gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga
      781 gacgtctcca agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc
      841 tttttaatag tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg
      901 aattgatctt aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga
      961 agatcaatac acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact
     1021 ttcgcgaagg acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg
     1081 atcattacca agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga
     1141 ttgaaaggca gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa
     1201 tcgacaagaa aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc
     1261 tatctgtttt gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat
     1321 ctaagtacta ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg
     1381 ttacgcctac acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc
     1441 aatacgaaat tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga
     1501 caaaaacgat tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact
     1561 agcagtttca attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag
     1621 agatatagtg aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt
     1681 tttcgaaatt acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc
     1741 ggagcattcg tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac
     1801 tgaaacagct aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc
     1861 atcacaatca acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc
     1921 aatcagcttt gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa
     1981 agaaaaaaac gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga
     2041 aatcgtgaag cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca
     2101 gaatctggcc actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga
     2161 gatcaaaaaa gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac
     2221 gaatgtagga gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga
     2281 ttggaagaca agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg
     2341 ggcaattatt ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat
     2401 cgattatctg aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc
     2461 aacggctgtc gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca
     2521 agtatcttta ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc
     2581 aaaggcttaa caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg
     2641 gacagttttc acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta
     2701 gaacgcgtcc tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca
     2761 ctacactaag gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg
     2821 gattggacaa aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga
     2881 aactagccat gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg
     2941 agctgttggc attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc
     3001 cggaatggca gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa
     3061 attacctaaa tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag
     3121 tccatttgaa ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca
     3181 tcgatttgaa cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta
     3241 cgcctatcca aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag
     3301 gtgcacgtag tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg
     3361 aattagctta tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag
     3421 aaatcgcaca acgtttttct gttgatctac atgctgttca gcagatattc gactatttac
     3481 aaacgttgag tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc
     3541 ctgatgtccg ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa
     3601 atcagccaaa tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata
     3661 aagaaactga aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga
     3721 tcctacaacg tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag
     3781 ctttctttat cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg
     3841 agatagatgt gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag
     3901 acttcggtgt atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag
     3961 aacagacaga agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc
     4021 aagaagacaa aaacaagccg ttgtctgacc agaaattggt tgaattgctg aaaaaagatg
     4081 agatagcgat ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt
     4141 catcaaaaag aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact
     4201 ttttctagtc tttttagttg aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt
     4261 tgagatttcc ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg
     4321 ggtcgtttta agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat
     4381 gaaataaaaa tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat
     4441 aaaattttac gaaatatcta ttggatgcag ccgattggac gtcgaagttt atcggaaagt
     4501 atggggatca cagaacgagt attgcgaaca gaaacagatc ttttgaaaaa tctgaatttg
     4561 atcgacacct caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc
     4621 cttgaaaact tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa
     4681 tattttggta accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg
     4741 gctgatgcat tcggcgaagc gctgagcgaa gccctagacc gactacttcc agaaggcgag
     4801 aatatcatcg ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat
     4861 ctggaaaata agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg
     4921 ataactgtac aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac
     4981 cgtgcacttt atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag
     5041 ccatctatcc aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt
     5101 gggcgtgcat tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg
     5161 aaacaagcaa atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc
     5221 gtttataagg tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc
     5281 atggcaatcg cgggcggaaa atcaaaagct aaagcaattc gagcttatat gaaaaatgca
     5341 ccaaaacaaa cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta
     5401 accctttaaa ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa
     5461 agtaggtatt aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt
     5521 agatggaatc gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt
     5581 gttaaaatac gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc
     5641 attcaacgta aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc
     5701 atggggcgaa ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc
     5761 agcagctgaa aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg
     5821 aaacgatgtt ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac
     5881 agttatctca ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa
     5941 cgacaaattt ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca
     6001 aatgactcta gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc
     6061 aaacatcgtt cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcctgaatt
     6121 gaacggtaaa ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga
     6181 attagtaaca gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga
     6241 agcttcaaac gaatcttacg gatacaacac agacgaaatc gtttcttcag atatcgttgg
     6301 tatgacttac ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa
     6361 acaattagtt aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt
     6421 acgtacttta gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata
     6481 gacatgtaca tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag
     6541 tttattcagg aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca
     6601 aaaaagttct tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg
     6661 acactcgtat caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag
     6721 caatcctttt ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt
     6781 tagctccagt tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg
     6841 aaactcgcgg agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg
     6901 tattcgaaaa tacacgattt gaagatattg acggtaaaaa agaaagcaaa aatgatacag
     6961 aactaggtaa atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag
     7021 ctcaccgtgc acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg
     7081 gattcttgat ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgac
     7141 caatgattgc aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact
     7201 tgattcctaa agcagataaa atcttgatcg gtggcggaat gacttacaca ttctacgaag
     7261 caaaaggaat caagatcggt aactctctag tagaagctga taaagtggaa ctagcaaaag
     7321 aattaatcga aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag
     7381 aattttcaaa tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg
     7441 ctttagacat cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa
     7501 cagttgtttg gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa
     7561 tcggtgtttg tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag
     7621 actcagctgc agcagcagaa caattaggat ttgctgacaa attcacacac atctcaacag
     7681 gtggtggcgc aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca
     7741 acgataaata aacgaccaag tttgctcaat tctgacgagt aatcgatcaa aaggtgaagg
     7801 gagttttcat gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt
     7861 aaacctatta tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt
     7921 gctgaagcag taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct
     7981 cctgcattat tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca
     8041 gcacaaaact gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct
     8101 cttgctgatc taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc
     8161 catgaaacag acgaagacat caataaaaaa gcaaaagcta tctttgcaaa tggtatgaca
     8221 ccaatcctat gctgcggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg
     8281 atcgaaggac aaattacagc tggtttagct gatctttcag ctgaacaagt gtcaaacatg
     8341 gttattgctt atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc
     8401 gctgacgaaa tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta
     8461 tcagaagcag ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac
     8521 atggcaaaag aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca
     8581 tttttgtcat tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa
     8641 tgtttgcact aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa
     8701 aacaaactca aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat
     8761 cttagactca cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt
     8821 tggccgcgga atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg
     8881 tgacggagac aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa
     8941 caacatcatt gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa
     9001 agctatgatc gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt
     9061 aggtgtttct atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca
     9121 ctacctaggc ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg
     9181 cggatcacat gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc
     9241 tccaacattc aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat
     9301 cttgaaagct cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct
     9361 tggttcaaac gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt
     9421 acctggtaaa gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga
     9481 aaaaggtgtt tacgtactag ctgactcagg cgaaggcgaa aaaacaactg acgaaatgat
     9541 caaattctac gaagaattag tttctaaata tccaatcatc tcaatcgaag atggattaga
     9601 cgaaaacgac tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt
     9661 agttggtgac gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg
     9721 aattgctaac tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga
     9781 agctatcgaa atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga
     9841 aacagaagat tcaacaatct ctgatatcgc tgttgcaaca aatgctggcc aaatcaaaac
     9901 tggttctcta tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga
     9961 ccaacttggt gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa
    10021 ataattcttt tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac
    10081 ttagttaagt aatcgaactc tctacttgct gaaatatcag caggtagaga
//
LOCUS       DAMYXM010000081        10055 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.084, whole genome shotgun sequence.
ACCESSION   DAMYXM010000081 DAMYXM010000000
VERSION     DAMYXM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.084"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            43..2208
                     /gene="acm"
                     /locus_tag="PNS21_002399"
     CDS             43..2208
                     /gene="acm"
                     /locus_tag="PNS21_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HDI5160060.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPGATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2480..2965)
                     /locus_tag="PNS21_002400"
     CDS             complement(2480..2965)
                     /locus_tag="PNS21_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDI5160061.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3055..3400
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3445..4086
                     /locus_tag="PNS21_002401"
     CDS             3445..4086
                     /locus_tag="PNS21_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDI5160062.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4105..4740
                     /locus_tag="PNS21_002402"
     CDS             4105..4740
                     /locus_tag="PNS21_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDI5160063.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4758..5573
                     /locus_tag="PNS21_002403"
     CDS             4758..5573
                     /locus_tag="PNS21_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI5160064.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5892..7328
                     /locus_tag="PNS21_002404"
     CDS             5892..7328
                     /locus_tag="PNS21_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDI5160065.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7517..9964
                     /locus_tag="PNS21_002405"
     CDS             7517..9964
                     /locus_tag="PNS21_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDI5160066.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
BASE COUNT         3499 a         1664 c         2171 g         2721 t
ORIGIN      
        1 tagtaggttt tatttttagt taaaataggg gagatagaaa aaatgaaaaa gtgtagaaat
       61 attattttat caatgttatt tattattact aatataacat cactgattcc tgttcatgtc
      121 tatgcggatg caggcagaga tatcagcagt aatgtcactt cgttgaccgt ggacccaacg
      181 aatattacag atggaggaaa cataaaagta aaattctcgt ttgacgaaaa aaaacaaaat
      241 atccaaccag gtgattatct ttggatcaat tggccaagtg aaggaaatat tcgcggtgaa
      301 ggattccaaa aagaaatccc attaatgatt gaaaataaaa atgttggaac tttaacggtc
      361 agaaaagatt ctgcgcaggt tgtgtttaat gaaaatatta aaaacctaga ctcagtagaa
      421 ggttgggggc aatttgagat acaggccaga aacgtaaccg ataccggtga agaaaatacg
      481 ggaccattca ccgtgacgag cggtgataaa acagctactg taaatgtgac aaagccagct
      541 tctggttctt cttctagcgt tttttattat aaaacaggag acatgctacc tgaagatact
      601 aagcacattc gatggttttt gaatattaac aataacggca cttatgttga acaaccagtt
      661 aaaatcagtg atgaaatcca aagtggacaa agattagatc caagtacgtt tgaaattaat
      721 caaatacact taggtgaaca gaaggtttat agaggagaag agggaatcca acaattttta
      781 caagatttcc ccggcgctac atttaatttt tcagtaactg ataattacat tgaaataact
      841 ataccaaaaa actttgtcaa tcttaggaaa ataatggtga gttataagac gatcatagaa
      901 aacccagaac aaataaattt tgagaatcat tcggaagctt ggtttaaaga atttaataaa
      961 cctgctgttg atggagaaag tttcaatcat acagttaaaa atatttcagc ttctggtgga
     1021 gtcaacggta cagttagagg cgaattgaag attttcaaat atatcaatga cacagagatt
     1081 ggtatcccaa atgtgacttt cgaattaaga cgagcagatg aacagcctat tcaaggacag
     1141 tctagtatcc tgttgacttc gaatgaacag ggagaagcta gtatcaaagg cctgcaagta
     1201 ggagactatg ttgtaaaaga aaaagaagca cccaattgga ttgattttga tccactaagt
     1261 tcaaatgaac tcaaattctc cataaacgaa aatgatacag agggagtcag tttacctatt
     1321 tataacaaga aaaaggttac gaacatcaca gcaactaaaa tttggaacgg tggaaccact
     1381 ccaagaccga gtatttattt taaattattc agagcaagta caaacaattg ggaaccagtt
     1441 cctgatgcag aaacaaaacg attagacaat ggaataactt cagtgacttg ggaagatatc
     1501 caacagtatg atgatagtgg aaacgaatat acgtttaaag tgcaagaagt agatcaaaac
     1561 ggaaatgatt atgtcccttc aggttatcaa aaaattgaaa acgggctagt cgttacaaat
     1621 gagaataaag aagtcatttc agtctcagga caaaaaacat gggaagacaa tgagaaccaa
     1681 gacggcaaac gaccgacgac gatcacagta aatctattag ccgacggtca accgatccaa
     1741 cacaaagaag taagtgaaaa agatgattgg cgctatcgtt tcacaaatct gccaaaatac
     1801 agagacgggc aagaaatcat ctatactgta acggaagaca acgtgcccga atacagtacg
     1861 acgattgacg ggtataacat caaaaacagt tatacaccgg aaaaaacaaa tatatctata
     1921 ccaggtagcc atataacacc gtggaaaaat tttccagaga agacagagag agagaaaatt
     1981 agaaatctat tctctcaaaa attgcaaata tttgctcata caggagaatc cagcgaacaa
     2041 agaagcaatt atattagtaa gagagctgta cctaaacaga taacaaataa atcaaaaagc
     2101 attcttccta aaacaagtag taaaaaatct agttttgctg taatcattgg tttattgata
     2161 gtaactatct gtgctgggat attcttacaa aaatataaaa aagtttagtt agaatataat
     2221 attttttgtt aaatatgact gaaatcaaaa gaataaaatt ccgatttata atcgaaaaaa
     2281 gaagcatttt tatatatgct tcttttttcg tagaatcaat aaatatggat ttggctaact
     2341 atggaagaat actcgtatta gttttcagcg ttcatactag ttgttacatt gtttgattgg
     2401 ccaaaagaat ttctgatata ttttgtgcta aataaagaaa aagacgcttg aattgggaaa
     2461 taagaaaact actctatcat tacaaatttt cataaactgg cagatgttct aagtaaacaa
     2521 gtatcttttc aaagtcggaa aaatccataa tcaacgtagt ggtattgata tttggatgaa
     2581 agcctacggt atcttgacga tcaatagcag catcgatcac gacttggatt tttttctctt
     2641 tgtcgtgcat caatgctaat ggagtcactg tacctgctgg aacgcctagg aggtcaggaa
     2701 gctgttcttc agaaacaaaa gataagcgct tttcttttaa ttgttcagct agttgtttta
     2761 aatcggcgtg cttttcatca ggcaagatga caagatagat ttgttttcct tttcttgatt
     2821 tcaataatag attttttact tgcggaccag gtagggaaaa agggacattt ttgactgacg
     2881 taatcgcagg atgatcgact ttttgaaaag agatacccag tttttttagt agttcataag
     2941 cttcttgttc ggttgcacga ctcatcgcgc atcaccctcc tttttttatt agtgtatcat
     3001 aaaaaagagt tgacaaatga aaaataatca tgtaaattac acgtaaagtt aaaacaaatg
     3061 acatcgaaaa gaagagtagc cgttaggtgt ttttagagag tctctggttg gtggaaagag
     3121 gcagcagtta accgtgaaac acatcttgga gtcgattttc tgaagcgtat agtaggaaaa
     3181 tccggggagc ccgttacagc tgcggtcgtt gctagatgac cagataaggc tggcttttcc
     3241 gtgaggagtc ggtaaaaaaa ggtggaacca cgtgattaac gtcctttgat cttcggatca
     3301 aaggactttt tgttttcatc tgaacggcaa cgactgtgtg aggttttttc tgcaaggaaa
     3361 aaataaacag aggtggaacc acgtataata cgtcctcttg gcatttgcca agaggatttt
     3421 tttattttaa aggaggaaaa gaagatggag tatttgattg aagttatgcc gcaattatta
     3481 gtaggagcac tcacaacatt gaaattattt ttatttacct tgattggctc attaccatta
     3541 gggattgttt tagcattagg attgtctttg cattttaagc cgctacgcta cttgcttcaa
     3601 acatatgtat gggttatgcg gggaacaccg ctgttgttgc aactgatttt tattttttat
     3661 gggttgccga cgattgggat cgtttttgat cgtttcgaag cagcactcat tgcctttatc
     3721 ttaaactatg cagcttattt tgcagagatc tttcgtggag ggatacaatc gattcctcaa
     3781 ggtcaatatg aagcagcgca agttttgcaa ttaagtccct ttcaaacggt gacgaaaatc
     3841 gtcatcccac aagtgacaaa gattgtttta ccttctgtag ggaatgaagt tatcaacttg
     3901 gtcaaagatt cttccttgat ctatatatta ggtttaggtg acgtaatgcg tgctgggaaa
     3961 atcgcgatgg aacgcgacgc gactttactg cctttgctag gcgtggcaat catctatctt
     4021 acattgactg gtattctaac aatcgtaatg aaacaagttg aaggaaaatt aaattattac
     4081 cgctaactga taggaggaag aaatatgatt gaattgaaga aagtgaacaa agcatttggt
     4141 gaaaaaaaaa taatcaaaga tttgaatcta atcattccag atgggaaaac attagctatc
     4201 gtaggtcctt ccggtggcgg taaaaccacg cttttacgta tcttggctgg cttagaaagt
     4261 tctgacagcg gagaatttta tttagacggt gtaccgttca acccattcga gcttaaaatg
     4321 aaagaacaag ttgtaggagt cgtattccaa gacttccagc tatttccaca tctgaatatt
     4381 tttgaaaaca ttaccttagc accaaaaatg gtattgaaac agacaaaaga agaatacacc
     4441 aaaaaagcag aagaattagc ggcttcatta gggattagtg atttattgaa tatctatcct
     4501 tatcagcttt ctggcggaca aaaacagcgg gtagcgattg ccagagcatt ggctatgaat
     4561 ccacgggtat tggcttatga tgaaccaaca agtgctttag atcctgaatt acgccagcaa
     4621 gtcgagcgtt tgatcctatc attgaaagaa gatggggtaa cccaaattat tgtcacccat
     4681 gatttagtat ttgcagaaaa tgtcggtgat caattactaa aagtttcccc aattcattaa
     4741 gaaaggagaa gatagagatg aaaaaaacca gttttctatt cgctttgatc gcaggtcttc
     4801 ttctttttgc aggctgttcg aacaaaaaaa cttcagcaga taattgggcg actatcaacg
     4861 aagagaaaaa aatcgttata ggtttagacg atacatttgt tcctatggga tttagagata
     4921 aagacgggaa gctcacagga tttgatattg atttagccaa agcagtcttt gaacaatatg
     4981 ggattacggt agactttcag cccattgact ggtcaatgaa ggaatttgaa ctgaacaatg
     5041 gaacgatcga tttgatatgg aacggctatt ccaaaacgcc ggcaagagaa aagaaagtgc
     5101 aatttacaaa accttatatg gaaaacgatc aagtgctgat cacacctaaa gaatcaggaa
     5161 tcacaaactt tggacaaatg aataataaga gattaggtgc gcaaaatggg tcatctggtt
     5221 atgatgtatt tacgaaacaa ccagaagtat taaaagagat agtcaaaaac caagatgctg
     5281 ttttgtatga tagttttaat gaagcattga tcgatctgaa aagcagacgg gtcgatggtc
     5341 tgctgatgga taaagtatac gcagactatt atttgaaaca aagaaacgaa ctagaagact
     5401 tcaacataac aaaaggttct tatgagagtg aggattttgc agttggtgca cgcaagtcag
     5461 atagtacatt agtgaaaaaa atcaatgaag ccctagatca actagagaaa acaggtgaat
     5521 tccaaaaaat atccgataaa tggtttggcg aagatgttac gccgagaaat taacaaatag
     5581 gggactgtga caaaagacgt gtcacagtcc ctttttccga ataaacagtg ttctcctgcc
     5641 aactccttgt gactcggagc tgaatggcag gctcacaacc tcttggtaaa cggtgttcaa
     5701 aagccagttt ttcggtatta agccaaattt atgcaaaaat gtagaaagcc attttcacaa
     5761 aatttttctt actactcgaa actaagcgac tttttcacaa cctcctttac ataattttat
     5821 tttgttgtgg aaggggtttc gtgttattct aaaagaggaa cgattgaagg ttataggaaa
     5881 ggaagagatg aatggaacac aaagaactta cctcatttcc aaaagatttt ctgtggggct
     5941 ctgcttctgc agcgtatcaa gtagaaggag cttggcaaga agatgggaaa ggagaatcag
     6001 tctgggatcg atttgtacgg atacctggaa agacatttaa aggaacgaac ggtgatttgg
     6061 cagtagacca ctatcatcgt tataaagaag atattgcttt aatgaaagaa caaggtctga
     6121 aagcttaccg cttctctgta gcttggacac ggatttttcc taatggacga ggagaagtca
     6181 atcaagctgg gctcgctttt tatgagagat tgatcgacga attgattgaa aacgatattg
     6241 aacctatgtt gactttatat cattgggatc ttccacaagc attgcaagat gaatataatg
     6301 gttgggaatc acgtcagatc attgaagatt tcactcatta tgcagaaaca ttatttgagg
     6361 cattccgcgg gaaagtccat tactggatta gtttgaatga acaaaatatc ttcacttcat
     6421 taggttattt gttagcagca catccgccag gtgtgacaga cccgaaacga atgtatgaag
     6481 ttaaccatat tgctaattta gcaaatgcat ctgtcatcaa taaatttcac gagttagaaa
     6541 tgcctggaaa aatcggacca agctttgcgt atacgccaaa ttatccaatt gacagtaatc
     6601 ctgaaaatgt tctggctgct gaaaatgcag aagacctgat ggccaattat tggatggatg
     6661 tctatatgtg gggaaaatat ccgattgctg ccatgaggtt cttagaagaa aaaggatggg
     6721 caccaacgat tgaagctggc gatgcagaat tgctagaatc tgctaaacca gattttcttg
     6781 ggatcaatta ctatcaaacg gcgacgaatg cattcaatcc attaaacgga gttggtgcag
     6841 gaaaaatgaa tacgacagga aaaaaaggtt catcagaaga aactggagtt cctggaatgt
     6901 ataaaaaagt tgagaatcca tttgtcgaac gaacaaattg ggactgggag attgacccag
     6961 aaggattaag gatcggactg cgtcgtatca caagccgtta ccgcatgccg gtactgatta
     7021 cggaaaatgg cttaggagag tatgacaagt tgacagaaga taaacaaatc catgacgact
     7081 atcgaatcaa ttacttacaa agccacgtca aagccatcaa agaagcaatt tcggatggag
     7141 cagaagtcct aggctattgc acctggtctt atacagattt gcttagctgg ctgaacggtt
     7201 atcaaaagcg atatggattt gtatatgttg atcaagacga aacacaagaa ggatctttgg
     7261 aacgatataa gaaagacagt tattactggt accaaaaact gattgaagaa aatggagaga
     7321 acctttagtt cacatactta aaacaagaag gtacggctgg atatggccgt gccttttctt
     7381 gataaaatca tttagaaatt aagaaaaact gtaaaataag ctgaaaaatg aaagaaagcg
     7441 tcagttcttt caaacaaaac tttagttccc ttactttttt ttgtataatg aaacttgatg
     7501 aaatgaggga taataaatgg cacaaaagaa acggcctact aaaaaaagga aaaagacgaa
     7561 aaaaacgaaa aagcaaaaac agcagcagga acaaatgctg acgattggtg ttgggtttgc
     7621 cttgatttta tttgctgtct tcggcttttt aaaattaggt tttttaggaa tattgattgc
     7681 aaacggcttt cggatcattg caggaaatac ttaccaaatc ctctgtcttt tattagccgt
     7741 attaggattt tggattgtca ttaagaatac agagttttca attggtaaaa accgacgctg
     7801 gtttggcggc atactctttt atttcggtat cttattattg ttacatgccc atttatttgg
     7861 caaactccat actggggaac caaatatcat ggggacgaca tgggatttgt tagccagtga
     7921 tataaaacaa agtcaagtag acaataacgt tggtggtggg atgattggag caatccttta
     7981 tcattttact tacttcttga ttgcacagcc tggaagttat ttagcagcag tgctattact
     8041 ggcaggagga gcattcctgt tcagtaatct ggaaggctat cagctgttaa atggtatcca
     8101 atcaattgga gaacgagtac aggaattatt ggaaggtgat cctgccaagc aagctcgaaa
     8161 gcaagcggca aaggaagaga gaatgaaaca gcgcgcagaa gcgaaagaag caaggcgctt
     8221 agctgcaaaa gaagctgcag aaaaagaagc tgcagaatat gagaagaaaa aagcggtgag
     8281 ccagcaaagg gaaaatactg ctgatgaatg gcaggaacca gaaaatacag agccggagca
     8341 gctaagtttt gttccaatcg acagttttca agaaaatatc catcctgcga atctagagaa
     8401 accagtacca gatacaccaa aacagaccaa tacagcagaa ggtttttcag atgagttgcc
     8461 agaggatgac ggtacctcat tagaatttga gattgaagca gaacaggaaa atcaagacta
     8521 tgaattaccg acagttgatt tgcttgattc tataccaact gtggatcaaa gtgatgagta
     8581 taaaaaaatt gaaaaaaata tcggcgtatt agaacaaact ttccaaagtt tcggtgtcga
     8641 tgccaaggtc gtcaaagcca gcttaggacc atccgtcaca aaattcgaag tccagccggc
     8701 tgtaggggta aaagtaagta aaattgtgaa cttaacagat gatatcgcgc ttgctttagc
     8761 ggcgaaagat gtccggatgg aagcaccgat tccaggtaaa tcactgattg gtattgaagt
     8821 accaaatgga aagatcagta tggtttcctt tagagaaatc atcgaagcac aaccaaacca
     8881 cccagataaa ttactagaag tacctttagg acgtgatgtt tctggacggg tacaaacggc
     8941 tgatctttcg aagatgcctc acttactcgt ggctggttcg actggtagtg ggaagtcagt
     9001 agcaattaat gggattatca caagtatatt aatgcgtgct aagccccatg aagtaaaact
     9061 gatgatgatc gatccgaaaa tggttgaatt gaatatgtac aacggcatac ctcatctgtt
     9121 gacgccggtc gtgacgaacc ctagaaaagc agcgcaagcc ttacaaaaag tagtacaaga
     9181 aatggaagaa cgatatgaaa aattcgcggc aaccggagta agaaatattt ctggttacaa
     9241 tgaatttgtt caacagaaaa acttggaaaa cggaacgaaa catccaacct taccattcat
     9301 cgtagtgatc gtagatgaat tagctgattt gatgatggta gccagcaatg aagttgaaga
     9361 tgcgattatt cgtttagcgc aaatggctcg ggcagcgggg attcatatga tcctagctac
     9421 tcaacgtcca agtgtggatg ttatcacagg aatcatcaaa gcaaatgtac cttccagaat
     9481 ggcatttgcg gtatcaagcg gtacagattc tcggacgatc attgattcta atggggcaga
     9541 aaagctctta gggcgaggag atatgctatt cttgccaatg ggtgagaata aaccgatccg
     9601 tgtgcaagga gcattcattt ctgaccacga agtagaacgg gtcgttcaat ttgttacaga
     9661 tcagcaagaa gctcattatg aagagaaaat gatgccgaca gatgaagttg aaacgacagg
     9721 tgctccagaa cagccacaag atgagctgtt tgaagaagca aaagcacttg tcgttgaaat
     9781 gcaaacggca agtatttccc ttctccaaag aagatttcgg atcggttaca accgagcagc
     9841 acgtttagta gatgaactgg aagctcatgg agttgttggc ccgtcagaag gaagcaagcc
     9901 tcggaaagta ttcattgagc aggaagaaac atcagaaacg ttgccggacg ataatgacat
     9961 gtaaaacttg tgtgatgaag ggttgaaaaa cgaataacca ataaaaagac aaggcatgat
    10021 gataccaaaa gaaaaaatgg tttttttcca tcttt
//
LOCUS       DAMYXM010000082        10013 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.085, whole genome shotgun sequence.
ACCESSION   DAMYXM010000082 DAMYXM010000000
VERSION     DAMYXM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.085"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..1748)
                     /locus_tag="PNS21_002406"
     CDS             complement(<1..1748)
                     /locus_tag="PNS21_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HDI5160067.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCG"
     gene            complement(1761..2555)
                     /locus_tag="PNS21_002407"
     CDS             complement(1761..2555)
                     /locus_tag="PNS21_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDI5160068.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            complement(2566..3942)
                     /locus_tag="PNS21_002408"
     CDS             complement(2566..3942)
                     /locus_tag="PNS21_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5160069.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(4071..4718)
                     /locus_tag="PNS21_002409"
     CDS             complement(4071..4718)
                     /locus_tag="PNS21_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDI5160070.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(4802..6229)
                     /gene="uxaC"
                     /locus_tag="PNS21_002410"
     CDS             complement(4802..6229)
                     /gene="uxaC"
                     /locus_tag="PNS21_002410"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDI5160071.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(6303..7382)
                     /gene="uxuA"
                     /locus_tag="PNS21_002411"
     CDS             complement(6303..7382)
                     /gene="uxuA"
                     /locus_tag="PNS21_002411"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDI5160072.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(7385..9016)
                     /locus_tag="PNS21_002412"
     CDS             complement(7385..9016)
                     /locus_tag="PNS21_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDI5160073.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            9333..10004
                     /locus_tag="PNS21_002413"
     CDS             9333..10004
                     /locus_tag="PNS21_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI5160074.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
BASE COUNT         3210 a         1997 c         1327 g         3479 t
ORIGIN      
        1 aacccacaat aagcatctat tggacttatt cccttaaatt cagaaaggcc cattagtttg
       61 tccactaata tttctaaagt ttcttgtttt ccatcataag tattgatata agtttttact
      121 tgaggcacat cagctaaatg aaacggacac tgtacagata caaaaattgt tggaatttca
      181 tgaatataaa atggaatatc cggagtacct ttacttgctg gccaaacaat tctttgaact
      241 gtacctgggt taacattagc tatattaata atcaagtcat atttttcagt taaatctttg
      301 attggtctct tagcagcata tattccagct atatttttaa ttttttcttc ttgcgataat
      361 cctttcaatt cttcttcagg tgaaatgtat ttttcaactt caaatccttt tgctgacaaa
      421 atctgtttca ataaatcaat ggcctgaggt ctatttccag caactacttc accaaaccca
      481 ccttttaccc catgtacatc taccagtaaa atccttttat atttttctgg tacaataggc
      541 caaatatctt gtttatcttt aactagtgta attgcttgat cagctacctc tttaaaaatt
      601 gatttatttt caattaaacc aattttttcc atagcttctt gtttcggtaa caaaatttta
      661 ttcttttctt ttttatgcaa acctagttta gcctttaagc ctaaaattct tcttaatgca
      721 tcacgtaaac gctcttctga aataattcct ttgtaataac cttccatcat ataattaaag
      781 tcttcatctg gatcattaaa aaataggaat agatcacatc cagccgcaat agaagtgggc
      841 aataattctg accgtttcat tgccccagtc attcctaaca tatgactagc atcagtaact
      901 actaatccat taaacccgag ttttcctcta agtaagtcag tgattaattc ttttgataaa
      961 gttgcaggaa gtaaatcttc atctttcaga tcaagattaa aatgttttga ataagctggc
     1021 atatggatat gacctgccat aattgaaggt aatccagctt ctattaagcc tgagtatact
     1081 tttccaaaag aattatccca ttcttctgta cttaaactat taatactaaa agatagatgt
     1141 tgatctcgtt cgtcaatacc gtcaccaggg aaatgtttag ctgcaggcaa aattccactc
     1201 tcttgaatac ctttcatata agctaaacta agttctaaaa ctttatcagg gctttgtcca
     1261 aaagatcgac tagaaatgat aggatttctc caatttctat taatatctac aataggagca
     1321 aaactccagt tacatccaat agccgatgct tcaattccag aaactctccc taattcataa
     1381 gcatatttcg catcatttgt tgcaccaatt tttacttcta caccaacttc ggtaccatct
     1441 gtacaagctc cgttaccacc tgcttctgta ttagcagcaa ttaataaagg aattttgctt
     1501 tctgtttgta ggatatagtt ttgatcatat acctcatcag cagttcctgg attatatcgt
     1561 acagctccaa tatggtactg attgaccata ctacttaagt aatttttatc acgactagat
     1621 cccatattaa caaataattg acctattttt tcttctaaag tcatagcatc aatcgtctct
     1681 tcaatccaag aaatagcatc ttcatctaaa tgatatggtt tttttcgtaa gtctactttt
     1741 atagtcattt tctttctcct ctacttatta gtaagatttt tccaaaaagt atctatatat
     1801 ttttgttcaa tatctatata ctctcccgat aaacatttaa ataaaatatc agttcttaag
     1861 ttttcccatg attctttttc tttattaacc aaaataggat aaaagaaaac tttattctta
     1921 tttgcagctt ctagatcacc aggagaatca ccaatcatta atatcttatc caaatcacaa
     1981 ccattttcaa ttacacttcg aataatgtct tctttttttc ctttatcttg acaacacagt
     2041 tcatcaacta aggataataa ttcatgtctt tcccattctt cttgaatagc ttccttatta
     2101 gctgagctta caataattat cttcccatat tttttcagag cttttatagt atctctagca
     2161 cttaaaaagg gtttatctaa tcctttagct tgactaatct gtcgattgac ttcttccgac
     2221 cattttaaag cttttaataa atcctctgac tgagagtatt ctatttccct cttcaaagat
     2281 ggattagaca attcttttgt tacagatacc cagtgcttta atcgattaat cgatggtaca
     2341 tcattttttt ctaatgtttt aactaaacct aaaaatctgt ttattccacg agtctctgaa
     2401 tataaattaa tattattcca atcctgtagg aatttttccc tatgatgaac atcaaaaatc
     2461 tctgcagcaa ttggcccgaa aaagagattg tgtttataag tcattgtatc catgacacaa
     2521 ccatctgaat caatacaaaa tatccaactg ttcatgtttt ttctcctagt tatgtttttg
     2581 atttagcaaa ttataatttt ctaatacttt ttctttagat aatggatata cgaaaaataa
     2641 aatcaatcct acaacaaaga aacaaactgc tggaatcaac gttgaagagt tatacagctt
     2701 ttgtaaaact tctccacttt gtacttttgc taacgaatca taccctatta tagaaagagt
     2761 ccaacctccg acaccgccag ctaaagcttg tccaattttt cgggcaaaag aatatactgc
     2821 ataaatagtt ccatcttctc tttggccaga tttcacttct tgatcatcga ttacatcagt
     2881 aatattcgcc caaatgactg tattaaaaat acctaacccc atatatccaa cagaagtaag
     2941 aactaagaat acatacatat tactaggctt tatcaaaaat aaaagtaaat atacaatagc
     3001 agagaagaac atccctactg acgccaattc ttttttaccg tattttttac tgaaaattaa
     3061 acttacaggt gaaacaagta ccaacatcaa aataggattg ataaagttca ttaaaactat
     3121 tccctgtgca ttattataat aatctggaaa tacataattg ttcatactca ttgttaaaag
     3181 ctgagcaagc aacaaaaaaa tagctgcagc tattatagaa agcaacgctc tatttttagc
     3241 taatcctttt aaagatttca acaatgaaac ttttaactgc gattcatcac ttttaacccg
     3301 ttctacagtt aatttataac atattacgta acaaataact gcacacactg aaaatactcc
     3361 tgccacaatt gtaaaagttg atccaccacg tacaatttga gctccagaac tatctcttgt
     3421 ataaataaaa attggtgtta acacgccaat gaacatacct gctaaggtag ctcctatact
     3481 acgaaatgtt gataacgctg tcctttcttc tgcaacagaa gtgatagctg atgccattga
     3541 accgtaagga atattaatag atgtataaaa aatacttccc catagtatat atgttacata
     3601 catatatata actttaatac tcatagaagc tgatgccatt gatgtttgat acattaaaaa
     3661 acttgctaaa gcgactggac cactcatccg taaaatccac ggtttaaatt tgccagtttt
     3721 agatggtttt gatccatcca caatacgccc cattgttatg tctgtaactg catcaataaa
     3781 tcttgctact aaaaataatg ttcctacggt agctccgctt atccccaata cttttgtata
     3841 aaaaatcatt aaaaaagaac tagcaaaaat gaaagtgaaa tcattcccaa aatctccaaa
     3901 catatatcct attttgtctc ttagaccaaa ttttctttcc atataatttg cgctccttcc
     3961 tattgttaag cgttttcatt ctgttgaact gccaacttac gtactatact ctgaagtata
     4021 aagagttagt atttattttt tacattttat agtattattt attttttctc ctaataattc
     4081 tttatctcat aaaacttatc tatatattta ctagctattc gagttacttc ttcaaaatca
     4141 cctttttcaa caggagccaa caaattccca cctacaccaa ctgcaacaac gcctgcttca
     4201 aaccactctt ctaagttatc tagacttact ccgccagtag gcattatatt tacatatggt
     4261 aatggagctt gatacgtttt tacaacagat ggaccaaaag cagaacctgg aaatagttta
     4321 atgatttcta ctccagcggt aagtgcctcc tttatctcag taattgtcat acaacctggt
     4381 aaataaggaa tttgatataa attacaaatc tctgctgttt gtcgatcaaa agatggactt
     4441 acgatatatt ctgctccagc cataattgca atacgagcag taaacgcatc taaaactgta
     4501 ccggctccaa ttattacatc tttttgatca gcatattttt tagcaatttc tttgattatt
     4561 tcttcagcat tcggaactgt aaaagttagt tcaatccctt taattccacc cgcagtaatt
     4621 gcatttattg ctttaatggc ctcatcttta ttttttccac gtacaaccgc aatgactcca
     4681 gccgcttcta acttacttaa aatttctact tttttcatat ctatctcctt cttttatgga
     4741 tacatactct agctagttgt actaacttac aataaataaa tttaagcttt tattttttgt
     4801 attattttct actttcgatt tcaaaaaaat taaaataatt atacgcatta cgataacaaa
     4861 tattctccac tatctgacta agatacattt catcatctgg aattcgacct gctttaaccc
     4921 actcgcttag taattgacat aatactcgac ggaaatactc atggcgagga tagcttagaa
     4981 aacttcgaga atcagtcaac atacctgtaa aattcgacaa caaactttgt tgcgccatcg
     5041 tagtcaactg ttgacgcatt ccttcagctg tatcattgaa ccaccaagca catcccaatt
     5101 ggattttttg aatcataccc ccttggaagc tttgcattgc tgttgctagt ggaagccaat
     5161 cggttggatt taatgaatat aaaattgttt ttggtaagca cccactcttt tcagcagtgt
     5221 cgaacaatgc tacaacattt tgtgtcaaac tagcttgatc ccccatcgaa tcaccaccga
     5281 catctactcc aatttgttcc tttaatatac ttgaattatt tcttaaagca tttaaatgca
     5341 tttgcatcgt ccaaccatat cttgtatact cagacatcaa atgtaattga atacctgtaa
     5401 caaattgtgc aatttctgat aaatggaaat ccgtttttcc tgctaaagct tttattaaca
     5461 gattgtcgag tattttttca tctgctattt cataaaaata agtattcaaa ccgtgatctg
     5521 ctaatcgaca gccaatttga tgaaaataat ccactcgttg gctcaatgcc tgcttcaacc
     5581 cattaaagtt tttgattggt atacccgctg cttgactaag ttttaaaata tagttcgaaa
     5641 atcctgatga tttgatactc cagaccttat ctggacggaa agtaggtaat accttgaaag
     5701 aattatcctg tttagaaagc tcagtatgat atcgcaagtc atctgtagga tcatccgttg
     5761 tacaaatggc tttcacatcc atcatcttca gcaattcttt tgggcgatag gaatccgttt
     5821 ggattttttc gttggcacgt cgccaaattt ctggtgcatt cttctcagaa attgtcaaat
     5881 caatatcaaa gtaacgttgt aattccaaat gagaccattc aaaaatagga ttacctggcg
     5941 ccttctccac tgtttttaca aaagctaaaa atttttcata atcattacct gcaccactga
     6001 tcaactcttc ttctatccca ttagctcgca tcaatcgcca tttataatga tctccagctc
     6061 ctttatcatt taaccatacc tgagtcaaat tcttgtattg cttattttct agcaaacatg
     6121 ctgggtctaa atgacaatga taatcgatga ttggttggtg ttctgcaaat ttatgaaaaa
     6181 gtcttttagc tatttcattc tctaataaaa agttcttatc taaaaacata tttaagcact
     6241 cctttccttc ccttagtttt tagaagagaa aatttttctt ctaaatagac tccattatat
     6301 aattatttac tcgccccctt ttcaagcgct tcccaaatac catttaaata aactgcacct
     6361 agagctcggt catataaccc ataaccaggt ctaccggttt ctccccaaat catccgacca
     6421 tgatctggac gaatatagcc attaaatcca ttatcgtaca ttgtttttaa tatcttatac
     6481 atatctagag aaccatattc tgatttatgc ggcgcttcat aaaaatcgag tccattatca
     6541 cttaaatatt tgatattacg aacatgtgca aaaggaatcc gatcccgttg acaaaattca
     6601 gatagaattt catagacatc attatctggt cgttctgcaa tacttccagt acataacgta
     6661 ataccattag aaggagaatc cactacatta cacaaccatt ctaaatcgtc acgattttta
     6721 acaatacgag gtaatccgaa aatagaatat ggtggatcat ctggatgaac tgccatttta
     6781 atcccaactt cttcacatgt cgggataatt gctttgagaa aatatgcaaa gttctctcgc
     6841 aatttcgctt catcaacttc tttataggct tctaataatt gcttgatttg tgataaccga
     6901 tgaggttccc aacccggcaa ttcaaaatta ttagtagaat tttctacttt tctaataatt
     6961 tcctctggat cagtaggaat attttttgaa atataggcta acgtagaaga cccatcatct
     7021 aaatgataat ccaaatctga ttttacccaa tcaaagactg gcataaaatt ataacagata
     7081 acctcaatcc caaattctgc caaattgcga atcgtctgtt tataattctc gatgtattga
     7141 tctcgtgttg gcaaaccaat tttgatatca tcatgaacat taacactctc aataactttc
     7201 aaggtaaggc cattggcttc gacttcatct tttaaagctt taattcgttc tctcggccag
     7261 acctctccgg ctggtatatc cattaatgtt ccaacaattc ctttcattgc tgggatctga
     7321 cgaatttctt gtagtgagat tttttcttca tttgccccat accatctaaa agtcatttcc
     7381 atagttactt tcctccaact tctgttatat atttattgat tgttttacga actgcttgag
     7441 gacctactag catttcgcaa aagatttttt caattttctt tcctatctca acttgatata
     7501 aatctaaacc aaatatttcc ttattttcta agatttcctg taccgcacaa tgaatctttg
     7561 caggatcttg ttcacctaat ttcaatactg agaatttttc ctgcaactct accaataaag
     7621 gatcaggact tggcacaaaa ttttttcctt gatcatcgac tgccaataaa taacgtaacc
     7681 aagcagcaat agcgatagga ataaatgtta gattttctgc atttccgtct ttttcgacat
     7741 actttttaat tgtttcacca tagcgaatcg ctactttttg agaagtatct gttgcaattc
     7801 gttgcggtgt atctggaata tagggattag gcaaacgttt attgattact tcatcaataa
     7861 attgttttgg atcaataacg cctggacttt caactactgg taagccttct acataaccta
     7921 tccctttaat taagccaacc aaatcatcat cttgcatttc tgcctgaatt gatttatacc
     7981 ccaacaaaca acctgttatt gctaatgccg tatggagcgg atttaaacat gtcgtaacct
     8041 tcatgatatc tgctttatca actgtttctc tatctgtcat aatcacacca gccgcttcca
     8101 gtttaggtcg accatttgga aaactatctt caatgactag ataatggact ttttcagtat
     8161 tggtaaaagc agcgatattg gtatttttcg cagtatgaat tatatctaag tcttcgaaac
     8221 ccgccttcaa taaatccatt ttgacacttt ccgaaggatt tggcgtaatt ctatcaatca
     8281 tactccaagg aaaagtcact ttttctttat cctttaaata agcaataaat ttttcagaaa
     8341 cgtatccttt tttttgccaa gcttctgcaa tcataaaaat gctatcttga aaacgttgac
     8401 cattctgtga gaaattatca gtagaaacca tcgcaatcgg taactctcca gcattaaacc
     8461 tgctatacaa caaggatgtc acaatactca tcgtatgttg aggattggta ggtccagatt
     8521 ttatatctgc ttccactacc gaaaaaaatt gtcctttaga atcttttaaa ctgtatcctt
     8581 tttcagtaat agtaaacgtt gtaaactgta atgtaggatc ttcaaaatat ttaaccactc
     8641 tttgatagtc cgttatattc tttggattgc aatataaact atcagctgtt actgctaaaa
     8701 gtcttttttt gagttcacca tcttcaccca taacaatctc caaaatatca ttgttgtagt
     8761 catgataagc tttttctact acttcttcat caaatgtctc acacacaact actccggatt
     8821 ctaattcatt ttgatctgct aagcattgag caatttctgc atgaaatcct cgataaagat
     8881 ttcctccacc aaaatgaagc cacactggat gtttctgacc ttcttgtttg accgcttcga
     8941 cattaaaata cggtagttga acttgcattt tgttcaatcc ttcgatagaa tcttgccagt
     9001 ctttaatcaa aaccatattt acccctccat actgatatgt tagtttccgt tacaattgta
     9061 gtataaaatc gaatgtaatc gctgtcaata agaaaaaact aaaaatattc ttttttctac
     9121 catgtcagaa ttattgtgtc tttttttagt aaaagcacac caatatatat tttttagaac
     9181 taacttaaat taaagcttaa tttctctttg atcttatttg agtatttttt gtttttttaa
     9241 tagctagtat gttactttga aatttgatat tatataacga gcatagaact aaaatatcaa
     9301 attcaaaaat gaaattttga gagggattaa atatgacaat taaaactagt ctccaaaatc
     9361 aagcttatca atctattcgt cagcaaatta tctacgctga cttagaacct ggaaaaaaag
     9421 tatcagaaaa aaaattggaa gaaacgcttt ctatcggtcg tactccaata cgtgaagcat
     9481 tgattcaact ccgtcatcaa ggacttgttg atacaattcc tcaaagtgga aattatatct
     9541 cattaatcga cttagattta gcaaaaaatg ctcgttttgt cagagaacat ttagaacgac
     9601 aaattatgct agagtgttgt gccaagatga ataatcaaaa gaaacaagtt ttagaaacta
     9661 taattgcaga acaagaaaaa gctgttagtc aaaagaatgc tcgtagtttt tttcataatg
     9721 ataatttatt tcataaagct tgttttgaaa ttgcagaacg ggaagaagta tggcaatggt
     9781 tggaagataa taatacgcac ttggaacgat ttcgttggtt acgagtaaaa acggaaggat
     9841 taaaatggga aaaaattatg gaacaacatc atcaactatt taaagccttg accatgcaag
     9901 accctgaaga agctaacttc ttaacatcct tgcatcttca tttaatgctt gatgaacaaa
     9961 atgttgtatt gactactcac ccaaaatatt ttaaaaatat ataactgcta aat
//
LOCUS       DAMYXM010000083         9940 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.086, whole genome shotgun sequence.
ACCESSION   DAMYXM010000083 DAMYXM010000000
VERSION     DAMYXM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.086"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(168..1235)
                     /locus_tag="PNS21_002414"
     CDS             complement(168..1235)
                     /locus_tag="PNS21_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDI5160075.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(1255..1734)
                     /locus_tag="PNS21_002415"
     CDS             complement(1255..1734)
                     /locus_tag="PNS21_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HDI5160076.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(1751..2212)
                     /locus_tag="PNS21_002416"
     CDS             complement(1751..2212)
                     /locus_tag="PNS21_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDI5160077.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(2226..3476)
                     /locus_tag="PNS21_002417"
     CDS             complement(2226..3476)
                     /locus_tag="PNS21_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HDI5160078.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(3854..5303)
                     /locus_tag="PNS21_002418"
                     /pseudo
     CDS             complement(3854..5303)
                     /locus_tag="PNS21_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
     gene            complement(5624..6388)
                     /locus_tag="PNS21_002419"
     CDS             complement(5624..6388)
                     /locus_tag="PNS21_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HDI5160079.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(6416..7114)
                     /locus_tag="PNS21_002420"
     CDS             complement(6416..7114)
                     /locus_tag="PNS21_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HDI5160080.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(7126..7905)
                     /locus_tag="PNS21_002421"
     CDS             complement(7126..7905)
                     /locus_tag="PNS21_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HDI5160081.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(7921..8865)
                     /locus_tag="PNS21_002422"
     CDS             complement(7921..8865)
                     /locus_tag="PNS21_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDI5160082.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(8911..9690)
                     /locus_tag="PNS21_002423"
     CDS             complement(8911..9690)
                     /locus_tag="PNS21_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160083.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         2868 a         2069 c         1508 g         3495 t
ORIGIN      
        1 gtaccgtgct tgtactcttg ctctattaca agcactaaaa cactactatt catttgaatt
       61 cttacacgga taaaaattta catcgtctat ttcaatattt tttctctcta ttttattcac
      121 ttaataagtt cattacataa tcgaaacatt ttttattttc cttgtcatca ctccttgctc
      181 cctttttgat acagatttaa aattctatca aagaaatttc catcgttaaa tttcgttatt
      241 gccaaatcct tcgaacaacg tgaaaatttt acgtatttat ctttatcatt gatttttagt
      301 attgcagatg tcaacgctga tacgtcacca ggttgaaaca aatatccatt ttctccatca
      361 cgtactgctg aagaaatatc tcctacatct gtagcaatta caggcattcc ataagaaatc
      421 gcctctagaa tagatatagg caatccctcg ttataggacg gtaaaatcat cacatttgat
      481 cttctatagc actcctcctt tttttctccc acaacccaac ctgtaaattc aaaatacgat
      541 tgcaaattat attcttctat caattctttg agcgatattt cttcatcacc tgctccagca
      601 atctcaaaaa taaacttttt ttctggatat tgttctgcaa tgtttttaac tgcttctacc
      661 aaatcgtgta caccttttcg ctttatcaaa actcccataa acagaaatcg gcacattttt
      721 gaattccact ttatggtata ttctggaact acaatagaat tttctacaac aacagtttta
      781 caatttggct caatttcttt aattatcttc ttccattttt ctccaagtac aatcgtggaa
      841 tcagactctc ttaacagcaa gcgaatctga ttttgttttt ttacagaaac actatcatac
      901 cattttttaa attccgatcc atgaagatgg atgattacag gaatatttaa taatctaaac
      961 cacttattca gcacatattt cctactaaaa ctccctctgt acgacatgtg catatgaata
     1021 atattaggtt tactcaataa agagatacct ataattttag caaatgcata cataaaatat
     1081 aaagatttaa atattgcatt tccaccctta taactcggta taaaagtcat ctcgatattc
     1141 tttttttccc aattaaaatt gagcatttgt gtaattatac ttgtaattcc accttttacc
     1201 gagctgtcat taccaatcat aagtactctt tgcataacgc tttactcctt atatttatat
     1261 tttttctatt aatacctcta tcgaattaat aatcgtttcc gtgttagata catacttgtt
     1321 aaacatccga actcttgttt ctttaacggc atcttctaaa ttttctaatt cataacaagg
     1381 atagataata ttcatctcat caaattgtct aattaactgc agttggtgat catcgacatg
     1441 ctcaccatac ttagccaatc gaggaacagc aatgaccctt ttaccttttt ttacggcccc
     1501 aattatagca cctgtacctc catgagtaat caccatttct gctttttgca tagtttcagt
     1561 aaactcatcc ctatttagaa atgctttata ggaataattt agtggtttat aatcactata
     1621 accactctgt gcaaatgtac tttcgattat tactcctttt tcaattaaat catcaatagc
     1681 ttttaacaat ctatcaaatt gaaatttttg tgaacctaat gttataaata tcattttttt
     1741 ctcctattat ttaatatatc ccacccaaat aaatagcatt aggataaaat tgtagcatag
     1801 tttcccactg aacataaaat tgatctgcaa aatgatacat caattttcct gtctgagtag
     1861 ggctggttac ttttgcaaac gattcaatat aaatcagttt ttttcccatc atttttgtta
     1921 ttaagcagat agggatcata gctaaaacac ctgtacaaat cacgagatcc ggtttttcct
     1981 ttatatatat aataattgat ttgaataaat taataagtat agtacacaaa aaaaactttt
     2041 cttttctatt tacttgtctc atgtagtacg ttttttcgcc ttttgactca gctgaatatt
     2101 ctgtctgttc tgttataaca aaactctcat acttctccat cagtggtttt aacatcatta
     2161 actgttcata atgcccccca gaggaagccg caaaacatat ctttttgttc atattaacct
     2221 ctctatcagt cacgctgaaa aatatctcta gtgtaaactt tttctttaat atcatccaag
     2281 caggtatcat atcgattggc aataatagaa tcacacatcg ccttaaactg ctctaaatcg
     2341 ttgataacct tacttccgaa aaaagtctct ccatctttca gtgcaggctc ataaatcacc
     2401 acagtcgcac ctttagcttt tacgcgcttc ataactccct ggattgaact ctgacgaaaa
     2461 ttatcactgt tgctcttcat ggtcagccgg tatataccga ttaaaactgg attttccctg
     2521 ttgacatcat actgattata ttcagcgtag ttgtaatagc ccgccatctg aagaacacga
     2581 tctgcgatga aatccttacg ggtacgatta gattcaacga tagcctcaat caaatttcca
     2641 ggaacatctg catagtttgc caacaactgc ttggtatctt ttggcaagca ataacctcca
     2701 tatccaaagg atggattgtt gtagtgagtt ccaatacgag gatcgagaca aacaccattg
     2761 attatctgct gagtattcag ccccttcatt tcagcataag tgtctaattc attgaaatag
     2821 ctgactcgca gtgctaaata ggtatttgca aacagcttta ctgcttcggc ttcagtgaat
     2881 cccataaaaa gagtatccac gttctcctta attgcacttt tctgcagaag tgttgcaaaa
     2941 acatgagcag catttaacaa gcgctcatca gtcatatcag taccaacaat aatacgagat
     3001 ggatacagat tatcatataa agccttgctc tcgcgcagaa actctggact aaaaataatg
     3061 ttcttactcc ccgttgtctc acggactgat tcagtgtagc caacaggaat tgtagacttg
     3121 atgaccatga tggtatcagg attcacgtcc atcacaacct caataactga ctcaacagca
     3181 gaagtatcga aatgctgagt acgactgtca tagttagtcg gtgtagcaat aacaacgaaa
     3241 tctgcggatt tataagcgct ccttgcatcc aaagtagcag ttaagtcaag ttctttttct
     3301 gtaagatact tttcaatata ttcatcctga attggagact tccgtttatt aatcagttca
     3361 atcttttcag ggataatgtc taccgcggtt acctggtgat gctgcgccaa tagtgtggcg
     3421 atactcaaac caacataacc ggtgccggct actgcgatgt tgtacttttt cttcacaata
     3481 tctcctcttt cctctcttaa gtatggagaa tgttactcct acggctattc atcatgtcaa
     3541 aataacacag cttagaatgt tgataaaata attttccaga atgtgataaa atctctttca
     3601 tttataaacc acacatctaa ttttatctat tctccacaag acatgtagag tattctttaa
     3661 taacaagata cgaccttact aaatacttca aaaaattatc atatttaatt cataaatata
     3721 aatcttcatt ttaaatgtat gcccaaaaat ttatctgtaa tgcgtcatca aaatctagat
     3781 tccaaatctt tatcaatttt tatccactca ttctaccact ttagtaatat tttctattta
     3841 taacactttc gtcctacatt gatccccccc tctgaagtac agtttttact gtcttaaata
     3901 aaattttaaa atctaacccc atactccaat tttttatata ttccgtgtca agttttgcta
     3961 cttcttcaaa atcagtaata tcacttctac cacttacctg ccacattcca gtaattcctg
     4021 gcttaatagc aattcttgca cgatgatgat aatcatattc ttcatactct gatttaaggc
     4081 atggtcgaaa tcctataagg ctcatctttc ctgttagaac gttgaaaaat tgagggaatt
     4141 catctaaaga cgtggatcta ataaattgac cgattcctgt cttttttgtt ccatctggaa
     4201 gtattttatt tccgaattct tggatcaaac tccatcttaa acataagatt attgctcatc
     4261 ttattttctt tcattaattc tacttttctt tcttctgcat ccatatacat acttctaaac
     4321 ttatacatct tgaacggttt accattctga cctatacgtt cttgtgcaaa gaaaattggg
     4381 cctggtgaat taatataaat aagtggagca ataaagataa aaataattcc cgttgcaata
     4441 catccaacaa tacctgcaag aatatcaatt gttcgtttta taaatgcctg ttgagttgtc
     4501 ataacattga tacttgttgt aagtactgta tattctccta tacgaccaat agattgtttt
     4561 cccaaagcac tatctgatac ttttgcaaga ttcaaatgta cagtaaggcc catttcagag
     4621 catcgttcaa taaattcttt catacacgaa gtacttttat tcaaaataat aaagacttca
     4681 tctacccatc cctgacataa gtaatccgct gcatttttgg aatcagaaac aactgtcact
     4741 ccctcaacat cttcaccaat catgctttta tctatgataa tgacaccatt tattttatac
     4801 attttatagt tatgctcttt cacattacga ataactactt cagcaatact actagatgta
     4861 actataataa gtgatttgtt cccctcattt ttcattttat gtttcaagta atgtttccat
     4921 aagaggcgag ttatatatgt aattacaacg tataaacctc ccatcaagaa aagaacgact
     4981 ctagaataat catttccacc ctgaacacta atgagataca aacttcctaa cagcaccaac
     5041 ataatcgctt gctttacaga agaagcaaat tctatatagt accctctttt taatacacct
     5101 ctatatgctt caaacatgaa aataactaaa agatctgcca gttcaatgaa gactgccatg
     5161 ttttgataca ttggaatcac ataaggattc caatctacct ggcgtaagac atacgataga
     5221 taaaaagcaa gctggataca caataaatct aaaataataa aatcaatatg tttaacccat
     5281 ccccttgaac ttttcttgta cattcctttt tactcccctc tttttaataa aataaattgt
     5341 attacttgtt ttctctccta aatatctttc tgaaggatta ttgactcgac aaattggctt
     5401 gtggatattc ttatatatag aacatccgta agccaactta aaactcctta acaaaatttt
     5461 tttactcata ttgtagatca aaacaagtat ccaagaaatc atattttatt tcttttttct
     5521 gcttactcaa caacagctaa aagaaaaatc atcataaaca aattcaacta agccaacgat
     5581 ccccttgtga aatcaagttt tttcttgatt tacgaataaa gagctaaaat aaaccaaact
     5641 tcttcttttt tacctcttca tagactggat aactgatttc atccccgttg atcagatcct
     5701 ttgcgacttg tttcatctgt actacttttt ccttaccaaa gtcctttgca atctgctcat
     5761 agcactcttt taagtaaaag gtgcgcttag agaccccatg ggcatcagaa gcagccatct
     5821 gcacgagatt atgtttgacc atcgttttgg ctgttttctg gatgtctttg ccaaataccc
     5881 caacataact tggcgcagtg agttgggcca aacatcccat atctaggaac ggaagcaaat
     5941 gatttgggtc ttcgcgaaaa tgcacgttgc gttctggatg gacgattact ggtgtctttc
     6001 ctagttctaa caactcaaaa aaaagccgtt ctgcatataa cggcacttcc agtgtcggaa
     6061 attcgatcag aagatatgta tcatctaaat ctgtaaataa tatttcatct cggtggatgt
     6121 cttcgatgag cgttccggta atacgtactt cttgtccttc caataacgtc aaaggcaact
     6181 gtcttttctc taattcggct tgtaacgaag cgactagaga aatgacttgt gatttttcat
     6241 tgctgtattt tccattattg tggtgtggtg tgcataaaat atgcgtgatg ccttgctgaa
     6301 tcgctttttc agccattgca atacttgctt ctaagttttc tgccccgtca tcgattcctg
     6361 gtaggatatg gcaatgtaga tcgatcattt ctcttgtctc ctctcttctt attccttaat
     6421 ttccgtagta ataaccagca tctttgctat gttctgctcc gttgtacaca acacctaaga
     6481 ctcgtgcttg taccatctcc agcagttctt ttgctttagt caaggattct ttacgtgaca
     6541 tattttcacg tacgaccaat aaagtgccat ccgtttttaa agacatgatc tgtgcatctg
     6601 ttacggctac tactggtggc atatcgaata tcaccacgtc aaataactgt cttgcttcgg
     6661 ataggatctg atccattctt ggcgagctta acaattctga tgggttgggt ggcttaggtc
     6721 cactaggcaa aatcgacaag ttctcgactg gcgttcgttg gatcacatct gctacacttc
     6781 ctgatgaact aagtgctgta cttaatccac tggcattgtt cagctggaat gtcttataga
     6841 cgacgggttt acgtaaatct gcatcgacta gtagaactcg ttgacctgat ttagcaaaaa
     6901 cgactgcaat gtttgctgca gtggtggatt tcccttctcc aggacctgat gaagtcacga
     6961 caatcgtttt gatttgctga ccagctgcgg aggcaaactg gatattcgtc cggatcgtac
     7021 gatattgttc ggcgattggt gaagatgggt ttgttaatgt gatcaaactg actgcatgag
     7081 tttgtgctgc tttttgtttc tgtgttcgtg ccatttttct tcctcctaga ctcttgagcg
     7141 gcttctgcgt gatggggctg ttttatcgtt tgtagctttt ggtcgtgcag gagctgttcc
     7201 ttttttacgg ataccagcgt tcaattcttt cgctgacatt tctggaactg ttcctaagat
     7261 tgggaatcct aatgtatctg tcacatattt ttcatctttc actgtccgat cgagtaattc
     7321 caataagaat gccagaccta cacctaccat cattcctaac attaagccag tcattaaatt
     7381 caatttatta tttggcgaaa caggtgtcgt attggcaact gcacctgaaa tgatcgagat
     7441 tttatcaaca ttcatcacat cttttgcgtt ctcttggaaa acttgtgaag ttgtgttagc
     7501 gatctgttga gcagccactg cattcgtact tattgcctga atcgagaaca tttgtgaatt
     7561 ttctgcctga ttaactgaga tggcatcttt gatttgttca gcagtcattt tcacattgtc
     7621 ttctgtttct aaacgatctt tgacttcatt gagcactaaa tcactgacga tcatatcttt
     7681 atacgtattg atcatttgca ggttcgtatt gacatcattg acatttgcag tgtctgattg
     7741 tggcagtgtc acgatcaatt gtgcttgtga actgtatttt ggtgtcaaga tgaaaaatgt
     7801 tacgattcct gagatggcca agccagcaaa cattgagatg atgattatta ccaagtgttt
     7861 gcgtaatgtt tcaaaaattt ctttcaagct gattgtctct tccatttttt ctccactttc
     7921 ctatttattc tctagattca tttgatcttt taattctttt ttcacacgat caagttcttg
     7981 atcactgact ttttgatagg agatattatc ttgcatgaag ccttctcctt gcagctgatc
     8041 ttgcttaatc gtaccaaatg cctcccgata atctaaagca atttttttca tttcatcaaa
     8101 gttcatgtct gttttcatat tcgactctag agcattcaaa acggattgat agttgctgac
     8161 accgtttaga ctcaatactt ttttcgctac gccttctacg atctttcttt ggcgcccttg
     8221 tctaccgtaa tcaccatttg ggtcttcata acgcatacga gaataagata atgcctgatc
     8281 cccgttcaat gtaattttac cgattggaaa gtcatagcca tcttgtgaga aagtgaggtc
     8341 attattgact tcgataccac caactgcatc aacgagttcc tttagtcctt tcatattgat
     8401 agagacatag tgatcgattg ggatgtctac atatttttgt acggtatcca tcgccatggc
     8461 agctccgccg aatgcgtaag cgtggttgat cttgtctttt gtgtcacgac ctacgatgtc
     8521 gacatacgta tctcgaggaa tgctgacgat cgttgtctgt ttgtcttcag gactgactgt
     8581 ggcaaccatc atcgtatctg atcttccttg atcgacccga cctaaatctc cagtatcaat
     8641 ccccatcaat agaacagaga aagattcttg tttgtccaga tcaactggtt tcgttcgttc
     8701 tccttgggtt cgttccactg attcgtaagt tttctcaata gaatgtgaga gatccagata
     8761 taattttgct cctactccta caattgctaa tactaatacg ataagaactg ctaaaataat
     8821 caaaaatatt tttttccctc ttgattttct tcttcgtctt gccatagttt cctccgtttg
     8881 ctaaccttgc ttgaatcaac gttttaactg tcaatagctt atattgctat agcccatttg
     8941 ctgagcaaca gtttgtgctt ttgttgcgta tgcggctctt attttttcat tccggatctg
     9001 ctctatttga ctgagcgccg tcaaatattg atcgtaagta gcggtagctg ttacttgacc
     9061 gtctttcagc atcgtatcga atagctgatc tagttgcgtg acttgtgcaa tttgggtatc
     9121 agcgaatcct agatattgca taacgactgt tttccaagca ctatccttaa aaaagctcat
     9181 attttcacgt acatgggcga catctgcact tgccaacttt tctttgatga tgacatcatc
     9241 cacagcttgc tgccagtttg atacgttttt agtaaataat ttattgacag ccgtctggat
     9301 cttcagtttt gattctgcat cctctaactg cgttaagact gctttctttt cttcttggat
     9361 cgcagcagct tctttaggta attctgattc cttgatgtca taatcatctg ccgaaagttt
     9421 gatagaagat agtttatgtg acaaatttga aactgtttct tctgttattt caggatttag
     9481 gaaaattttt tcttttgtac tgtacagctt atctacttct tttgataaag aggtcacggt
     9541 gtctttttga ttggtgatgg cagattctgc gtaattgatc cgttgatgat attgccgctg
     9601 attattgaga tggtatccaa acaagcaaat cacaatagcg cctgtgccta ccgccacagt
     9661 agccaataac ttgacgaacg gcttttccat agattcactc ctaaatattg atgatagata
     9721 agtaaagatg tgattcattc acatcagaat gagcgctttt tattttaaaa gcataattaa
     9781 actaatatat tcatagaaat atttataaac aggttttccc tatataatca taccacatac
     9841 agtttttttg actagggaaa ttcctaattt aaaagtaaat aaaacagtaa caagattata
     9901 attaatcgtt ttcattttta tttcctcact tttcatgagc
//
LOCUS       DAMYXM010000084         9889 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.087, whole genome shotgun sequence.
ACCESSION   DAMYXM010000084 DAMYXM010000000
VERSION     DAMYXM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.087"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            205..684
                     /locus_tag="PNS21_002424"
     CDS             205..684
                     /locus_tag="PNS21_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160084.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            717..2945
                     /locus_tag="PNS21_002425"
     CDS             717..2945
                     /locus_tag="PNS21_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI5160085.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3074..4717
                     /locus_tag="PNS21_002426"
     CDS             3074..4717
                     /locus_tag="PNS21_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDI5160086.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4790..5335)
                     /locus_tag="PNS21_002427"
     CDS             complement(4790..5335)
                     /locus_tag="PNS21_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDI5160087.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5358..5843)
                     /locus_tag="PNS21_002428"
     CDS             complement(5358..5843)
                     /locus_tag="PNS21_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDI5160088.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5833..6645)
                     /locus_tag="PNS21_002429"
     CDS             complement(5833..6645)
                     /locus_tag="PNS21_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HDI5160089.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6784..8316)
                     /locus_tag="PNS21_002430"
     CDS             complement(6784..8316)
                     /locus_tag="PNS21_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDI5160090.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8425..9456)
                     /locus_tag="PNS21_002431"
     CDS             complement(8425..9456)
                     /locus_tag="PNS21_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI5160091.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3290 a         1834 c         1903 g         2862 t
ORIGIN      
        1 aaatattcca acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga
       61 gatggtgttt ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct
      121 ctataatgtc gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa
      181 ataaaaggat gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta
      241 atcgttgtag aagactatga taaagttgat ggaagaaatg cattaaatat ggatgcaaaa
      301 gggctttcac tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag
      361 gtatggcgac atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt
      421 attttagatt tagcgatctt agctgcgcaa ggaagtttat acttccaaga tgcttatcgt
      481 tatgaaaaat tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac
      541 cgaatgacgg tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat
      601 tacgatacac tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt
      661 cttttagatg agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg
      721 agaatttctc actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga
      781 agaaaccgaa aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag
      841 ggaattggca agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt
      901 cggggaagac acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag
      961 tgacctgttt tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag
     1021 attctaataa ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg
     1081 aagctgtact gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa
     1141 aattagcaga gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct
     1201 attacccttc tgaaatcgtt cgagcgattg aaaaaggcta tttattagag attcaagagc
     1261 cgactgtcat ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg
     1321 ggatgttgaa tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca
     1381 ctacaaatcg aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc
     1441 aacatgcgga aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga
     1501 aaactatgat ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg
     1561 atgaaactgc aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt
     1621 gggcaaatac ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt
     1681 ataaattatc cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg
     1741 gattgctact ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg
     1801 agcatacaaa agaaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa
     1861 atgctgaaga aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg
     1921 agggagaaga gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa
     1981 aagaacgcag tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt
     2041 ctcaagaaat gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga
     2101 ataaagaagc gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc
     2161 accgaccagc tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc
     2221 caatagtaga atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga
     2281 cgtaccaaaa aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg
     2341 atttgagaaa ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg
     2401 tacggatcga tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag
     2461 cagcgattgc cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag
     2521 atacagcaga ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag
     2581 acggatttca atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg
     2641 atggtgctgc acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac
     2701 tgttgcttaa tattagtgac ggtcagccaa aagcattgcc ggattataca ggcaaaaaag
     2761 caaaagaaga tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct
     2821 ccgcagctat tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg
     2881 tagatattac tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc
     2941 tatgataaaa taaagctcaa cgaacgcact tagcagattc gttgagcttt tcgtttctag
     3001 ttaccggtaa caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa
     3061 ggagtgaaac gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat
     3121 ctatcctgct agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg
     3181 tgaaaaactt aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta
     3241 tcaatctcca tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag
     3301 attcggaacg atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa
     3361 agtgattatg gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc
     3421 gaaaaaatct aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg
     3481 caaagaaccg aataattgga catcgatttt cggcggttct gcttgggagt attctcatga
     3541 aactggacag tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag
     3601 cgagaaattg aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga
     3661 tggatttcgt ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac
     3721 agagaaccct ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt
     3781 gaaacatgtt tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc
     3841 agaagaagga ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga
     3901 tcatatccat atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaaca
     3961 tgcattaagc gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga
     4021 aaaccatgat gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc
     4081 tatgtctgca aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta
     4141 tcaaggacag gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc
     4201 agtagattcg aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc
     4261 attggatatc attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc
     4321 ggaacaatat gctggttttt ctacacatga accttggctg atcactaatc caaataccaa
     4381 aacgatcaat gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa
     4441 catgatccgc attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct
     4501 tcatgaacat ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat
     4561 tatggtaaac ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc
     4621 ctggacatta ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc
     4681 accttatgaa gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta
     4741 gttggacttt gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg
     4801 ctctaggacc accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa
     4861 gggttttcaa acgaggtctg acaggtactt ggacatgctt gatatgcata ccgattgatg
     4921 tgtcaccgat atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga
     4981 actggtcaaa agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga
     5041 caatttcgaa attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat
     5101 gttcacaacc ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac
     5161 ggatgatcca ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt
     5221 cgcttgtgct gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata
     5281 tgtcattaac tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct
     5341 aaaaccttct ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt
     5401 ataggaaagg caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc
     5461 actccagccg gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact
     5521 aagcttccta ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca
     5581 gcaacatatc cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct
     5641 gaaatcaaat cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca
     5701 taaagaaaag cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc
     5761 ggtacaggga tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag
     5821 atcgttaatt gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac
     5881 acttccggta tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt
     5941 actttctgct actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga
     6001 agaaaaggca caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac
     6061 ctcataatga tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc
     6121 gcctttgatc acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat
     6181 ttctgttaac gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg
     6241 cgtaatcatc gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt
     6301 gatcacagct tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg
     6361 ttttgttttt atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat
     6421 tttgatacca tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc
     6481 caaagatata gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa
     6541 ggcgcttagt ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc
     6601 accagcaaat ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat
     6661 gaataagagt ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct
     6721 catccaattt aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa
     6781 tagttaggac ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt
     6841 agctgttcgc gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc
     6901 atggacttgc tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat
     6961 cggttgtgga gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga
     7021 atctaataaa aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg
     7081 ccgatctttc aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca
     7141 ccaatcgcca taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg
     7201 tcctttagac agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga
     7261 atctgtaatc cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact
     7321 tgttttcaaa ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc
     7381 atatacttga agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt
     7441 ctggttgttt atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg
     7501 atgctcctta gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat
     7561 tggccggtga gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat
     7621 cataggaaat cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg
     7681 agagatgtga aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca
     7741 tttcgtccgc catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt
     7801 accagaagta tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga
     7861 caaagcaggt tgttccatta aataaagaat aggaatctgt tttgattttg ttccatttga
     7921 aatttctctc actcgctttt ttgtctcatc atcaaataaa atgaaagggt cttctaaacg
     7981 atcgataaat acttccattg ttgttgttgg tagatgataa gaagtcatga caggcactgc
     8041 accaaggtaa gacacagaaa cagctagaag gtatgtgtca aaagcggaac tcttataaat
     8101 aataactttg tctccggatt tcactccaag atgagccaat tgataagccc gtttcaacac
     8161 actttcgcag cattttttgt aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg
     8221 ctcatcaaag ataatcgggg tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa
     8281 taagttcaaa ggggaatacg ttatggtctc catcattcac actccattat cttttgttaa
     8341 gtaatataca aattcaagat aatcgtaaca taatccttta tataataaca tagtttattg
     8401 tgaaaaaaag atcatacatt tgttttatat ggaagaaacc cgaacgaatg ccgctaattc
     8461 gtgataagaa aaatcaacca tcttttccca gatatcttga aaagaatggg atgagaggta
     8521 tcgattacca cataaataat ttaccgcgtc agcatcatct aaatatagtt ctgcataata
     8581 acgatatgtt tttctgcata gttctggcga tacgttttta tatactgtaa ttttgctcaa
     8641 aatttggcga atagtccgag gagaaatttg ctgatcatgt gaggaacgaa aaacaaaaga
     8701 attttcgtcc gtattttgtg ctgttaactt aaaatagttc tccagaaatg gcatcgcttc
     8761 ttttggtatt ggtactttat tcagtctgtt ttcttttcta ttcagattaa gaatctgctt
     8821 ggtcaaagat acatctttac gtttaagttc tactacttca tggatcttta atccgcaata
     8881 cagaagaatg gcagtaattg caaggttcct cggtctattc ttttcttcaa agctagcttc
     8941 tctgcttgtt tgaaagcctt caatcgggat aaacaaatgc tcaaaatctt tgaaaagttc
     9001 tgcatcgata tatcgcggag ctgctttggt tgtttccggt aaacttgtac ccaaaagaga
     9061 aatctcttct tcaatcagtc ctttttgata caaaaatcgt aaaaaatgat ttaatgaata
     9121 ttgcttcctt gaaatcccgt aatagctatt ttcaaacaat tgctccgttt cttggttcgc
     9181 tgtagtttgg aacgaacgag tatgtcgata gattttagct aagaaattct gataatcttg
     9241 tttttctaaa tggataatca gctgatcatc cacttcttca gaaaatactt ttctcaaata
     9301 atcagtaaac agatgaaaat cttttgcgta ttcatagcga ctgcctaatg aaagggaaga
     9361 acgattttct tctaaaaact gttgaaagac ggaagggagc aagtgtgtac gttgctttaa
     9421 cttttcttgc agttgttttc tagttttgct gtccattaca aattccctcc aatagtgttc
     9481 attttctata ttttaccgca tttcatcaaa tgaagataga cagtttgtct tgatcttagg
     9541 aagcaaattt ttaactaagt actagctaaa agatatgaag taagaagata catttttagt
     9601 tattttatta gtgcagtcat tcttttgatc ttttaattga tttatcctct ttttagctat
     9661 gtggttactt catgagcaat gaaatggatt tggttcgttc taatggcagg aggctctttc
     9721 ttttatttcc gaaaaattag aaaacatttt ttcagaatcg attggctctc catttattca
     9781 ataatggttt gtctaacgat attgttggga taattcttaa agaagtatta gaaatagttg
     9841 gtacttcatt actttacatg aagatttatt ctatggtagg agcaattat
//
LOCUS       DAMYXM010000085         9830 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.088, whole genome shotgun sequence.
ACCESSION   DAMYXM010000085 DAMYXM010000000
VERSION     DAMYXM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.088"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..205)
                     /locus_tag="PNS21_002432"
     CDS             complement(<1..205)
                     /locus_tag="PNS21_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160092.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(221..835)
                     /locus_tag="PNS21_002433"
     CDS             complement(221..835)
                     /locus_tag="PNS21_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDI5160093.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(982..1665)
                     /locus_tag="PNS21_002434"
     CDS             complement(982..1665)
                     /locus_tag="PNS21_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDI5160094.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1774..2232)
                     /locus_tag="PNS21_002435"
     CDS             complement(1774..2232)
                     /locus_tag="PNS21_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDI5160095.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2316..4079)
                     /locus_tag="PNS21_002436"
     CDS             complement(2316..4079)
                     /locus_tag="PNS21_002436"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDI5160096.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4300..5760)
                     /locus_tag="PNS21_002437"
     CDS             complement(4300..5760)
                     /locus_tag="PNS21_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160097.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="PNS21_002438"
     CDS             complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="PNS21_002438"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDI5160098.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6917..8344)
                     /locus_tag="PNS21_002439"
     CDS             complement(6917..8344)
                     /locus_tag="PNS21_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI5160099.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8375..9589)
                     /locus_tag="PNS21_002440"
     CDS             complement(8375..9589)
                     /locus_tag="PNS21_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDI5160100.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2917 a         2041 c         1628 g         3244 t
ORIGIN      
        1 atttgttaaa gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac
       61 catctaaagt aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact
      121 gcccttgcag aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg
      181 atggtttata gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg
      241 tgaaaatttc tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt
      301 ttcttggatc gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct
      361 gtacttctag caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt
      421 catataagga tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta
      481 ctgaatcagt taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg
      541 ctgctgtttt atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc
      601 ttgtctcaag aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca
      661 aggataagat gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca
      721 ctatatcttt tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa
      781 tcagcaactt gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt
      841 cgccttcttt ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg
      901 cttttttcat cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag
      961 aatctgtgcc gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt
     1021 agtgatcaat tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc
     1081 atatgctttc tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc
     1141 gacttgttct atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata
     1201 ttctgtatag cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag
     1261 cgcttcctca aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg
     1321 gacaagttca gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat
     1381 gatttctgct tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac
     1441 ttctgaccca tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt
     1501 gatttcattg atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc
     1561 agccaaagcc attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg
     1621 acaagaaacg acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt
     1681 aatgttatat aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa
     1741 taatgagcta tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc
     1801 gtatacgacc agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt
     1861 aaaaaactca gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt
     1921 ccaagcatgc tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat
     1981 gatacaaact ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa
     2041 aggctcattc tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt
     2101 tacaactagt tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc
     2161 taatttttct gattgataac taggatattt ttcttggaac tttttgacat attcttctac
     2221 ttgctgatac ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta
     2281 gtataaacaa ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga
     2341 ctatcttcaa cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga
     2401 acgctatccg tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca
     2461 taataacgac tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg
     2521 actaattcgt gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt
     2581 cctggattga tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt
     2641 cctaattcaa gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt
     2701 tgataggctg gaatactata atcatttgcg atagctgtac catgttctac accatgtgta
     2761 ggaattcgga ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc
     2821 atatcctctt ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga
     2881 atgtcaaatg ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa
     2941 ataatcactg gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc
     3001 aagaaagtac ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg
     3061 aaagtgatag agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct
     3121 tggaatgttt tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct
     3181 gtacttgttt gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca
     3241 gaagttactt gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc
     3301 attttattat cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca
     3361 atcggatgat caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt
     3421 ccattatcca cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta
     3481 ccgttggttt gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata
     3541 gacatctcat tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc
     3601 aatccttcaa aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt
     3661 cctgattctt tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta
     3721 gatgtgtgcc ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc
     3781 acttcacggt aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca
     3841 taagactgat gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga
     3901 aacgcaatcg ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg
     3961 tccttttgca agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat
     4021 aattttttga catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata
     4081 atacattctc tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct
     4141 attctactac ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc
     4201 ttgttatata agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa
     4261 ttaaaaagct ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa
     4321 taggtgaagt tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg
     4381 atagatcttt gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac
     4441 cactcttttt ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat
     4501 aggatttatt tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa
     4561 ttgtttgact gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc
     4621 actgttgatc aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag
     4681 ttggatttga tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag
     4741 ttgttctttt tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca
     4801 caacgtacgg acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat
     4861 ttcagcgata ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa
     4921 atttttataa acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt
     4981 tctactgttg aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc
     5041 tttttgctct tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt
     5101 cttatcttct tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc
     5161 tcgtatgata aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac
     5221 ataatcaatc aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc
     5281 ccaattgaca taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat
     5341 gatttcttta tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat
     5401 tgtatatact ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat
     5461 accattaaaa taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt
     5521 gagctcagaa agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac
     5581 ttgttctttt atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt
     5641 tggtgtagtc acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa
     5701 taattgttca cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat
     5761 tcctgataca cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat
     5821 ttttgtttcc tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga
     5881 caaaagtcaa acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac
     5941 ttttacccca gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac
     6001 tacatctttc aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct
     6061 gaagacacct atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct
     6121 ttttgcaggt acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt
     6181 tgtcataatt gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca
     6241 aggagcaatt cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac
     6301 attgagggag cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat
     6361 ttgattacct ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga
     6421 atgatagatt tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga
     6481 caagattcgc tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat
     6541 cgctcctgca ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat
     6601 gatagaattg aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat
     6661 tccgttgtct ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa
     6721 gcgcacatcg gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat
     6781 acctaattgt ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt
     6841 ttgataatag tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat
     6901 aaccagcttt cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg
     6961 taaatggaat aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat
     7021 gctgtccagc tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag
     7081 tcaaaagacc taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg
     7141 ctctttcaag actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt
     7201 acaacatcag gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc
     7261 ccccgccgct taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca
     7321 tcaaatgagc aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt
     7381 cttctgctgc tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga
     7441 tgacacctaa acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa
     7501 tgtgtcttaa taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt
     7561 gaggtactgt agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa
     7621 ccaatacaaa cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac
     7681 tggcagatgt ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag
     7741 gcaatttcga aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc
     7801 ctgaagcgac ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag
     7861 ctacaaaacc gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta
     7921 aaatagccca gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag
     7981 caccaatacc cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc
     8041 caactgttat ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg
     8101 tgcgactgac ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg
     8161 ctgcgcacaa tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta
     8221 agctgaagcc tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg
     8281 tcatagccaa aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt
     8341 gcatgaatct tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga
     8401 aaaagtccaa tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg
     8461 ccgattttaa aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa
     8521 tcacttgaat ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg
     8581 caaagatccc tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat
     8641 caagtgcatc atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac
     8701 ccgcaatgat cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa
     8761 tggctgccaa tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt
     8821 ttacttttgc aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac
     8881 caaatgcatt gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc
     8941 agccaaacca aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc
     9001 tttctcctaa aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc
     9061 cagctgtcgt gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa
     9121 atccgccacc ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa
     9181 caagtataat atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa
     9241 cggtaatcac gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac
     9301 catgaaacat gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc
     9361 caataacacc gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa
     9421 tcgttgttat cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc
     9481 gtgctaatcc gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac
     9541 ataaaatcat gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct
     9601 cctttctacg attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga
     9661 attaatactt gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt
     9721 tttcgttttt cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt
     9781 ttattttcgt ctttttttat ttttaaagat attttttcat aaggaataaa
//
LOCUS       DAMYXM010000086         9694 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.089, whole genome shotgun sequence.
ACCESSION   DAMYXM010000086 DAMYXM010000000
VERSION     DAMYXM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.089"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            185..1183
                     /locus_tag="PNS21_002441"
     CDS             185..1183
                     /locus_tag="PNS21_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI5160101.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="PNS21_002442"
     CDS             1295..1804
                     /locus_tag="PNS21_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDI5160102.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="PNS21_002443"
     CDS             complement(1834..2493)
                     /locus_tag="PNS21_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDI5160103.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="PNS21_002444"
     CDS             complement(2495..3259)
                     /locus_tag="PNS21_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDI5160104.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="PNS21_002445"
     CDS             complement(3267..3944)
                     /locus_tag="PNS21_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDI5160105.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="PNS21_002446"
     CDS             complement(3981..5366)
                     /locus_tag="PNS21_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDI5160106.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="PNS21_002447"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PNS21_002447"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="PNS21_002448"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="PNS21_002448"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDI5160107.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="PNS21_002449"
     CDS             6305..7162
                     /locus_tag="PNS21_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5160108.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="PNS21_002450"
     CDS             complement(7243..7578)
                     /locus_tag="PNS21_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160109.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="PNS21_002451"
     CDS             complement(7645..9015)
                     /locus_tag="PNS21_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDI5160110.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="PNS21_002452"
     CDS             complement(9093..9629)
                     /locus_tag="PNS21_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI5160111.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2847 a         1999 c         1619 g         3229 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaatat tatt
//
LOCUS       DAMYXM010000087         9667 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.090, whole genome shotgun sequence.
ACCESSION   DAMYXM010000087 DAMYXM010000000
VERSION     DAMYXM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.090"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..650)
                     /locus_tag="PNS21_002453"
     CDS             complement(<1..650)
                     /locus_tag="PNS21_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDI5160112.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIG"
     gene            975..1391
                     /locus_tag="PNS21_002454"
     CDS             975..1391
                     /locus_tag="PNS21_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDI5160113.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1388..1858
                     /locus_tag="PNS21_002455"
     CDS             1388..1858
                     /locus_tag="PNS21_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDI5160114.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1872..2645
                     /locus_tag="PNS21_002456"
     CDS             1872..2645
                     /locus_tag="PNS21_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDI5160115.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2659..3471
                     /locus_tag="PNS21_002457"
     CDS             2659..3471
                     /locus_tag="PNS21_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDI5160116.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3525..4490
                     /locus_tag="PNS21_002458"
     CDS             3525..4490
                     /locus_tag="PNS21_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDI5160117.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4606..7329
                     /locus_tag="PNS21_002459"
     CDS             4606..7329
                     /locus_tag="PNS21_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDI5160118.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELYITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7342..7974
                     /locus_tag="PNS21_002460"
     CDS             7342..7974
                     /locus_tag="PNS21_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDI5160119.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8873..>9667
                     /locus_tag="PNS21_002461"
     CDS             8873..>9667
                     /locus_tag="PNS21_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5160120.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLYL"
BASE COUNT         3261 a         1476 c         1885 g         3045 t
ORIGIN      
        1 ccaatcatat ggaagccacc gattaggtat tttatgggtt tagtcggaaa tgtttcaaga
       61 gcaatttcca tcatatcgat aatgccatga tgactacagc cagtaaagac aatcagaccg
      121 tcttcttgct caattacgac caataattca tctgaaaaat catcataaac aaatttatca
      181 tccatctttt tgtataaatt cttggtggtc gagtttgctt tgcctagggg gatgatatgg
      241 aagctcggag caagttctgt tttcgttttt attgtatgta aattctcatg cattttaagg
      301 atttcatttt taggaatacc aacatacaga aacgtggtta aaatctttaa ataatggggt
      361 aaagttacac cttcagctgt ataaataggg gcgtttcctg attttaaata tcttaacccg
      421 ccggtgtggt cagaatgacc gtgtgaaata atcactgcct ccagatcatt caaagtttcc
      481 cccattttag tcgcattata gactaatttt ttattagcgc cagcatcgaa gagatatttc
      541 ttccctgagt agttaattaa gagagataac ccatgtccac ttttaaagtt caaatttatt
      601 cttgagtttt caaataaaac cttgatttcc actataacat ctcctttcac atgaacgtgt
      661 attcatatat tgtagcacat taagttctat tttttgttgt aattaccgaa aaaaatatta
      721 atggacgctt tttacgcttt atttcgtatg tggaagaaaa tttcttagga tgtggcgggg
      781 gattggggca aagctaaatt ccaattacag tgatcattta attagacacc tttaaatacc
      841 cacactttca tcaacactat aatttaggga aagaaaagtg ttggcatgat atagaaaaac
      901 ctttaattaa cagcgtttcc taaaattggc acgcatcttg cttatagaaa agcgtaagga
      961 taggaggaat gaaaatggca actataattc ttgccagcca tggggaattt gctcaaggat
     1021 taaagcaaac agcgacaatg attattggag atagtgtacc aattcatgcc ttgtcagcat
     1081 ttagagatga agatgaatct attttggtac aaataaaaaa acttttagcc acaatagata
     1141 tagaagaaac ctacatttta actgatattc taggtggtag tgtgaataat gatatgctca
     1201 ctattattaa agagtatgag ggattgcaat taattactgg aatgaactta cctttagtta
     1261 tttctattgc aacctataat ggaaaaattg gggaaagtga gctagaaaag cttattaaag
     1321 agagtcaaca atcagtgatt gattgtagaa aattattagt agaacaactt actggaggag
     1381 atgacctatg attaagttag tacgtattga ccatcgtttg ttgcacggac aggtagtatt
     1441 ttcttggagt aaatcattaa atatcaatcg tattattatt gccaataaag aggcagcgaa
     1501 tgatgaattc aaaaaaatgt ctttgaattt atctaaaccg actggcatta aattaacaat
     1561 tttttcagtt gaacagttac tagaaaaaat gcctaaaatc gaaacactta acgaaaatat
     1621 catgcttatt tttggtaata caaaagaaat tcgtcaattt tgtgaagcct atcctaaagt
     1681 acaagaaatc aactatggag ggataatcaa aaaagaaggt tccaagcaat ttagtaatgc
     1741 cattttcttg aatgaatctg agcaagaaga tgccaaaaaa ttaaaagctc ttggcattaa
     1801 acaatttatt caacaggtac caacttctaa aaaagaagat ttgaatacaa tgttataaag
     1861 gaggaatgaa aatgctcgtt cagtcaattg gtttagggtt aatcggtgtt ttttgtattt
     1921 tagactcaag gcttttagga agaatgaatt ttgaacgacc gttgattgtc agtacgcttg
     1981 ttggtttggt acttggagac atggaaaaag gattaattgt tggagcctct ttggaattga
     2041 tgtccttagg attagtaaat atcggagctg ctgcaccacc tgatatgaat atggcatcaa
     2101 ttattgctac agcctttgcc attttatcaa atgcgaatgc agaagcagca ttaacgattg
     2161 ccattccaat ttcagtgtta ggtcaaatac taggtatctt aatgcggacg attttgtcaa
     2221 atttgactca cgttgcggat cgctttatcg aacaagggaa atttagccaa gcaaggagaa
     2281 ttcatatact ttatggctcg ggattatatg ctctcatgta ttttattcct atttttctag
     2341 caatttattt tggaacgaat cttgtttctc agattgttga tgcgattcct gagtggctaa
     2401 caaatggctt aacggttgca agtaaaattt taccagcata tgggtttgcg ttgttattac
     2461 aaacgatgtt aacgaaaaaa atggtgccat ttttattact tggattttta attactgctt
     2521 attctggatt gggaatcatt ggtattgcag ccttcgcatg tattgttgcg tttgtcatgg
     2581 ttcaagttca aggtaaaaac tcaaatggta accaaaatga gccagatcca ttagatgact
     2641 tataaggagg tagattaagt gaaagagaaa gatcaaaatg catggaaata tcggcagttc
     2701 ttatggcgtt catgggcaat tcaagcctca tggaactatg aacgacaaat gaacatgggt
     2761 tttatgtacg gaattgcacc aattctagat aacatttaca agaatccaga agatgaagca
     2821 atgaagaaag aagcttacga gcggcatatg atgtactaca actgtacccc tcaaacaagt
     2881 gcatttgtgt tagggttaac tgcttctatg gaggagcaat actacgaaga ccaagaaaac
     2941 tttaatccgg aatcaatcag tgcagtgaag acttctctga tgggaccgct atctggtgtt
     3001 ggtgattctt tctttcaagg aacgattcgg gtattagcct ttggactagg aattaattta
     3061 gcccaacaag gtagtatttt aggtccaatt ctagcaatgt taatttcctt tgtaccttcc
     3121 tttttagtta cttactatgg tgggaaaatt ggctataaca tggggaataa atacttgtct
     3181 aaactgtatc aagaaggttt gatggataaa gtaatgtatg tttgctccat tgtggggttg
     3241 atggtcattg gttcgatgat tgccagcatg atcgggatta caacaccaat tcaaatgggt
     3301 aaagcttttg tcttacaaga tgtattagat agtattatgc cacaattttt accattagga
     3361 ttaaccttct tcatgtactg gttattgcag aaaaaggtca aaacaggatg gatgttggca
     3421 atctgtttag tcggaggaat tatttttagt ttgttaggta tcttacaata agggaataac
     3481 caataaattt ggttgaataa tataaataag gaggaattca attgatgaat tcaaaagaaa
     3541 ttgttaaaga aatcgtagcg aaaaaagagg taaaatcaat cgtatttgtt ggctgtggcg
     3601 cttcaaaagc ggacctttat cctgcaaagt attttctaga ccaaaatgcg aagcagttac
     3661 ggattagtca ttacacagcc agcgaattta actatgcaac accagcatct gtagatgagt
     3721 caactgtcgt tatttcagct tctttaggag gtacgactcc agaaacagtt gaagctaacg
     3781 cagtagctaa agaaaaaggt gctacagtta ttagtctaac acgaagcatt gactcaccgt
     3841 taacaaaaga tgcagattat gtaatttatc atcgatttgc agagaactat gctgctaaat
     3901 tagaaaaaac aggctactgt ttagaattgg cagtagaact tttacatcaa gtagaaggtt
     3961 atgaccatta tgaagcaatg caagaaggat ttgctactat ctatgatctt gcccaaaaaa
     4021 gtgcagaatc agctcgtaaa gatgcgaaga aatttgctga acgctttaaa gatgaaaaag
     4081 ttatttatgt aatgagtagt ggtgctacac atgaagtagc atactcaaca tcaatttgct
     4141 tgatgatgga aatgcaatgg attaattctg gtaccttcca ttcaggagaa tttttccacg
     4201 gaccattcga aattgtggat aaagacgtac cgtttatatt attaatgaat gatggaaaaa
     4261 ctagaaatat agatgctcga gcattaactt tccttgaacg ctttgatacg aaaacagaag
     4321 ttgtagatgc cttggattat ggcttgtctt cacatattgc taaagaagtc ttagactact
     4381 ttaacccatt cgtgattact gcagtattta gagtttatgc agaagaattg tcagaagcaa
     4441 gagaacatcc attaactaag agacgttata tgtggaaact aacttattaa aaaatagatc
     4501 aaactaaata aggtggcttg agaaattaag ccaccttgtt ttcttcaatg tgattttata
     4561 catatggtaa aataactagt acatattgtg aaagcaggtg gcaagatgca gcgattaaat
     4621 gactatcttt atcaactaac attgcttttg aaatctacgc cttcagataa ttattcagct
     4681 cgcagtattt cgcaagcttt acagttgaat agaagtacaa tcagtagcta cttaaatgaa
     4741 ggtgttcgtg aagggctttt cataaaagta aaaagttacc cggttttatt tttacataga
     4801 acagctcttg aagaactata tatcacactg aataactccg aaatagaatc aattgaaagc
     4861 ttgttaacaa tttctcaaaa acccgcatta gatcaagtaa ttggctctaa aggaagtttg
     4921 aaagaagcaa ttgatcaaat taaaacggca gtcttatatc ctggaaaagg gttgccttta
     4981 ttattgatcg gcgctagtgg atcagggaaa acctttctag cgaataaaat atatgaatat
     5041 gcagtagaag aaaaggtcat tcacacatcg gcacctttca tagattataa ttgtgcacaa
     5101 tatgtgagca atccagagtt actatcctct gctttatttg gctacacgaa aggagcattt
     5161 accggagcaa gccaggagca tactggtttg ctagaaaaag ccgatggagg agtattgttt
     5221 ttagatgaag tgcatcgctt atctgaagaa gggcaagaga aattatttac atttatggat
     5281 acgggtgaat tctctcctat gggggataat agtattcgga aaaaagccga tgttcgatta
     5341 gtctttgcaa caacagaaaa tatatatacg actttcttac caacttttct taggagattg
     5401 ccagttattg ttaatctacc tagatttcaa caacgtccgt cgtttgaacg tctgtcattg
     5461 attgatgaat tttttgtttc tgagagtaaa attttagcta aggaactatc tgtttctgat
     5521 gcattgattc atttcttaat gaatagtgat tttgaaggta acgtggggaa tattaaaaat
     5581 attatcaagt atgcttgcgg aaacgcatat atccaccaga agagtcagca aacaattcaa
     5641 gttaaattat tggatatgcc tttagagtac agttataaat tcaaggagca actaaataaa
     5701 cccagaaaga aaagagcaga tcgaatctac tatccagaag aaacacgaca agtgcaacta
     5761 gaaagaaaca atcagctttt tgttcaattt tttgaccaat tacttactgg attctctgaa
     5821 gtgattgaaa ataacaagga tattaaacta tttttagaag aaatggttac caaggtaaca
     5881 caagtaatgg atacgcttat ttttcaagag gattacgaaa aagagcagtc tttatacgcc
     5941 attctaaact atcatattcg tcaaagtatt gattttatgc gagaaagcta cgggtttgaa
     6001 caagatggta accgggtagt cgctttggcc aactatctat atgtcaagga taatcaagtg
     6061 atttttgaaa atagtcttca agaaaagaaa accaaagatc agcttaacga attttttgct
     6121 aatgttcttg aaacccctta ttggtatgca aaaaaaatct taagcctttt atctgagtgt
     6181 ttagatcaac ctgtgtatga agaggacatt ttctttatta cgctatattt ttatagtttg
     6241 aatgtcacga atgttaaaac aggtattaag agtattgtgc tagctcatgg ttattctaca
     6301 gctagtagta tggcgaatgt tgccaaccgt atgctgaata agaacgtttt tcaagcttat
     6361 gatatgcctt tagatattac attagagaaa attgaagcaa aattgattca attccttgga
     6421 gattatcgaa cagattcagg cttgatttta ttggtagata tgggttcact aaaccaacta
     6481 ggcggtttat tagtgaatca tctaaatgga ccactgttaa tcattgatca tatcaataca
     6541 ccgttattat tggaagtcgg ccaacaagta ttaaacaata aaagtatcac cgaaatagga
     6601 gcagaaattg aaacaagtct tactttcaaa aagcagttac tacttcccaa gaaaaagaaa
     6661 aagaaagcca tcattacttg ttgttataca gggattggta gtgcgaccca gattcaggag
     6721 attttaacga aatgcttgaa agatgcagtt agagaattag taattattcc gtatgattac
     6781 aaaaagctgt tagaaaacaa gaattttgag actccttttc aattatacga tgtcatcatg
     6841 attgtaggta cagaagatcc caaaattaat cagattccat atattggatt agatcgcctg
     6901 attaatgggg aagcaattgg cgaatttgtt cattacttgg aaaaagagct gactattgaa
     6961 gtgaacgatt tacaaaaaga attaattttc aatttttcaa ttagtaaaat tgtcgaaaat
     7021 ttaaccattt tggatccaga taaggtgcta tcttttgtac aaaaagcaat taaagaatta
     7081 tcgaagttat taaattttga tttttctaat aatcagctgt ttttgttgta ccttcactgt
     7141 agttgtatga ttgaacgaat tgtacgtaaa gaaagtgtgg atgagcaaga ggacattgaa
     7201 gaatatatgc aagcaaatgg agaaataatg gataaaattc atagcgcttt ttttgagatt
     7261 gaaagggaat acacaattga aataccagat ttagagttgc gattattgag cgatattgtt
     7321 caaggatagg agtgaattcg aatgacaatg ggagtaattt ttgatatgga tggggtactc
     7381 atagacagcg agacatttta ttttgagaga agaatgaact tctttcaaga actggagata
     7441 gatccaggat caaaccagat tgatgattat gtggggaaaa cagaagctgc aatatgggaa
     7501 acattagttc ctgaggacaa atcattaaga gaggaacttt atccgaaata tgtagattat
     7561 cgaaaaacgc atccaattga tttcgctaaa gatttacgaa aagaagtgaa accgttactt
     7621 gacttcctca agaagcggga tataccattg gcactggcat cttcttctcc agttgttgag
     7681 atagagagta tgctcattca atgtgaactt actgaatatt tttcctttgt tataagtggt
     7741 gagtcattaa aacaaagcaa accacatccc gaaatatatt taaaagcgaa acagcaatta
     7801 gactgtgact tctttattgc agtagaggat tcaacgattg gtattaaatc agcaaaggca
     7861 gcaaatatct atacggtggc actaaaacaa ccaatgcaaa tcgatcagag tgaagcagac
     7921 atacaaattg aaagtctaca agaactggaa caaattgttt tagaaaaagg ttagtgaatt
     7981 aatggaaata aggctatata tctgtgctag ctattttgac taaccaaaaa aacttgctat
     8041 aattcaggtg tagtaaaagg tgctggtaac accttttacg aaaatgcttc ggcagtgata
     8101 atgggattgg ctttttaagt tttttatttt gttcgcaatc cctaaggtca tgtgaatgat
     8161 cctagggatt gctagaatat tttgcaacat tctatctatt tcagaacgta accttcccca
     8221 gttcattggg attcattagc atacaaccaa catttcaaaa agaaattgca ctacaccatt
     8281 attagctatt ttgactaacc ccaaaaaact tgctataatt caggtgtagg taaaaggtgc
     8341 tggtatcacc ttttacgaaa ctgcttcggc agtggtaatg agtgtggctt aaaggactag
     8401 tcctttaagc tttttacttt gttcgcaatc tctaagatca agttaatgat cgataagatt
     8461 gctagaatat tttgcaacat tctatttcag aacgtaacct ttcccattcc actgtgattc
     8521 attggcaacc ctcctcacaa ccaacgtttc aaaagaaatt gtaaagttgt tggggtacca
     8581 cgaccgaaca atactacacc tgaatgatag taagttgtct cattaagtat agcatatcct
     8641 ctttttgggt aaatagaaat cggttacatt ttttatccaa gaccaagttt tttcatttgg
     8701 tcttatttgc tatacccaaa caattccgca gtatagtcga cggtattgat tttatcaggt
     8761 gagtaataga aacttgtttt ccttccccaa aacattacct ataaaggaga aaaaatggta
     8821 tcgtaattat ataggtatag gatgaaattt ttttctaaga ggagggttgt tgatgaaaaa
     8881 tttacagtta aaatttattg ttgatccatc gattgtacgc tggtttcgga ttatcaatca
     8941 atttgagcgc aatcaacttg ccacaaaagg agaattagca aagagtaatc atgtatctga
     9001 acgaaccatt caaacagatg tcaataaaat gaaggactac ttttctgaaa gtgttcaatt
     9061 tgtttcaacg aaaagtgggt attcttttca gaaggaaaga ccggttcttt ttctcaagca
     9121 gaaggagcaa ctactggaga acgaaatttt atttgagttg ttagggaatg tcttttatgg
     9181 cgaattggaa gatatgacgg agctaattga tcgttttcat ttatctgaag cgacttttcg
     9241 tcggtatttg aagcagattc aaccagtttt ggaagaatac ggcttagaat tgagtcttta
     9301 tcctcttgat ttaaaaggag aagaggcaaa tattcgcaag ttttttaaag acttctatta
     9361 tgaaggggag atgacgcccc atactcttgt tccaccaagg gatcttcatg aacttgtgca
     9421 agctacattc tatgataaat ttgagtattt ctcgattgga acagggactt ctcctgctga
     9481 attttattat agtttgtata ttgcgattga gcgtgtgaga caaggaaaaa cgatagccat
     9541 tcctcaaggt ctgattgagc gagtaattgc tagtgagaat tttcgcttaa tgtattcctt
     9601 gaaagaagcg attcaaacaa attatcaagt tgctttatca gaagaggagt tttgttggtt
     9661 gtattta
//
LOCUS       DAMYXM010000088         9550 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.091, whole genome shotgun sequence.
ACCESSION   DAMYXM010000088 DAMYXM010000000
VERSION     DAMYXM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.091"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            168..362
                     /locus_tag="PNS21_002462"
     CDS             168..362
                     /locus_tag="PNS21_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160121.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="PNS21_002463"
     CDS             631..930
                     /locus_tag="PNS21_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160122.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="PNS21_002464"
     CDS             complement(1027..1461)
                     /locus_tag="PNS21_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5160123.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="PNS21_002465"
     CDS             complement(1693..2403)
                     /locus_tag="PNS21_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDI5160124.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="PNS21_002466"
     CDS             complement(2393..3253)
                     /locus_tag="PNS21_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDI5160125.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="PNS21_002467"
     CDS             complement(3258..3896)
                     /locus_tag="PNS21_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDI5160126.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="PNS21_002468"
     CDS             complement(3911..4210)
                     /locus_tag="PNS21_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDI5160127.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="PNS21_002469"
     CDS             complement(4253..5737)
                     /locus_tag="PNS21_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDI5160128.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="PNS21_002470"
     CDS             complement(5757..6218)
                     /locus_tag="PNS21_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI5160129.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="PNS21_002471"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="PNS21_002471"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDI5160130.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="PNS21_002472"
     CDS             7521..8291
                     /locus_tag="PNS21_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDI5160131.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="PNS21_002473"
     CDS             complement(8564..9391)
                     /locus_tag="PNS21_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI5160132.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2979 a         1947 c         1492 g         3132 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg
//
LOCUS       DAMYXM010000089         9398 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.092, whole genome shotgun sequence.
ACCESSION   DAMYXM010000089 DAMYXM010000000
VERSION     DAMYXM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.092"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..2202
                     /locus_tag="PNS21_002474"
     CDS             <1..2202
                     /locus_tag="PNS21_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDI5160133.1"
                     /translation="LADGQKGDNRLKTLKEIQQIYPQIPESKGSFPGYGGIPITHYKK
                     YWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAERQSSMVVNDPKTELYVASKDE
                     LEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDIDTATQLTNTFTNTIYYDANAS
                     DNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNNLVELLSEIGGFNYYPDPENEF
                     KQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNILSTAITGLNPFTLTKIAKMTS
                     QSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGKKFHEERVKVGYRGFCEINFDC
                     HLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKIVYRKKRGETHLPELEKEAVLT
                     LDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHIIVSIFLSQLYKELSTQCVKTK
                     GDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLWDLFIQSYQQLYAKYGEQNAA
                     IIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENSTINSLGMNTHINRNVDSDRIL
                     TPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHGETNLPYAYQYIEDFNPSWDI
                     NDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGLHDQLEEKNEENMFYKIVQSFE
                     NETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMIRKRLLLEAEKAVKE"
     gene            2199..4577
                     /locus_tag="PNS21_002475"
     CDS             2199..4577
                     /locus_tag="PNS21_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160134.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            4638..5180
                     /locus_tag="PNS21_002476"
     CDS             4638..5180
                     /locus_tag="PNS21_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160135.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            5258..5590
                     /locus_tag="PNS21_002477"
     CDS             5258..5590
                     /locus_tag="PNS21_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160136.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            5591..6184
                     /locus_tag="PNS21_002478"
     CDS             5591..6184
                     /locus_tag="PNS21_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160137.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            6206..8230
                     /locus_tag="PNS21_002479"
     CDS             6206..8230
                     /locus_tag="PNS21_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDI5160138.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            8230..>9398
                     /locus_tag="PNS21_002480"
     CDS             8230..>9398
                     /locus_tag="PNS21_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDI5160139.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP"
BASE COUNT         3298 a         1490 c         1961 g         2649 t
ORIGIN      
        1 ctagcagatg ggcaaaaagg agataaccga ttaaaaacgt taaaggaaat ccaacaaatt
       61 tatccgcaaa taccagaatc aaagggaagt tttcctggtt atggcggaat accaattact
      121 cattataaaa aatactggta tattcagacg gatacagtaa atacttgtat tgttggtact
      181 tcccgctcag ggaaaggcca gatagaagtt ttagcaacca ttgacaattt gtcgcgtgca
      241 gaaagacaaa gttcaatggt tgttaatgat ccaaaaacag aattatatgt tgctagtaaa
      301 gatgaactag aagctagagg gtatgatgtg tatgccttta atatcaccga tccactgcaa
      361 tcaatgagtg ataatccatt ggcactcatt gtgaaatact ggaagcgagg agacatagac
      421 accgcaacac agttaaccaa tacttttacg aatacgattt attatgatgc aaatgcttca
      481 gacaataaat actttaacga aaatgcgcag aaagctgtca atgcgcttat ttttgcgtta
      541 ttggaacaag cggatcaaac aggggattac tccaaattaa caccaaataa tctcgttgaa
      601 ctactttcag aaattggcgg ttttaattat tatccagatc ctgagaatga attcaagcag
      661 ctcaatgcct tagatgagtt catgaatcag ttaccccctg gaaatatagc aaaaaaacaa
      721 tatggggcaa caaaaattgg atctgataaa gcgaaaggga atatcttgtc tacggcaatt
      781 acaggattga atccatttac cctaacgaaa attgcgaaga tgacttctca atcgaccatt
      841 gattacaagt ccattggttt tccgaagtat ttggatctca aattacatga aagcctatta
      901 aatcaacgca ttgtcgtgga attttatcat aagggaaaga aatttcacga ggaacgagtc
      961 aaagttggct atcgtggttt ttgtgaaatc aactttgatt gtcatttaga gcagggagat
     1021 catgtttgct taaaatacaa tcatcaagga aaacaatatg ttgcttggta tgagttttct
     1081 caaaaagtat tgaaagacga agagggaaaa atcgtttatc gaaaaaaacg aggagaaact
     1141 catttgccag aactagaaaa agaagccgtt ctaacattag atgagaagaa aagtaaccta
     1201 ttagtagagt ctattcggat gcactatacc gataaaccaa tggctatttt tatgttaacg
     1261 cctgattatg atccatctaa ccatatcatt gttagcatct ttcttagtca gttgtataaa
     1321 gaattatcta cgcaatgtgt taagactaaa ggagataaat gtcaccgtag gattcatttt
     1381 attttagatg aatttggaaa tatgccaccg atggacaaca tggaaggaat catgacggtt
     1441 acggcaggtc gtaatatgtt atgggatctg tttattcaat cttatcagca actttatgcg
     1501 aagtatggag aacaaaacgc agcgattatc aaagaaaatt gtcagacaca tatttatatt
     1561 atgtcgacag atgataatac catcgaagaa ttttctaaaa aagtggggaa taagacggtt
     1621 gaacaagaga acagtaccat caattcatta ggtatgaata ctcatatcaa tcgcaatgtt
     1681 gattcagatc gtatcttaac tcctgaaaga atttctactt ttttagaagg tgaaacgatt
     1741 gtactcgctc ctttaaaacg acaagatttg aaaggtcata aaatacggcc gtatcctatt
     1801 tttaatcatg gggaaacgaa tcttccttat gcttatcaat atattgaaga tttcaatcca
     1861 tcgtgggata tcaatgatat agacatacat tgtacacatg cggacttgga tcttaaggct
     1921 ttacaaattg attatgatta tttcctacca gatgaatatg ctagaaatct ctatcatgga
     1981 ctgcatgatc aattagaaga aaaaaacgaa gaaaatatgt tttataaaat cgttcaatct
     2041 tttgagaatg agacggctaa aaaattgtta attcaatttt acgaagaaca agcaacggat
     2101 cgtcttcaag aatatgttga aaagttgatc aaaaatgaag aaatttctga aatgattagg
     2161 aaacggctgt tattggaagc tgaaaaggcg gtgaaagaat gatcattgga ggaattttct
     2221 ctggcatagc cgactggtgg tcaggtttat tcggagaagg ggatgcagat cccaaaaccg
     2281 taacgaaagt cttacaagat tatggtaatt atctgcagta tcaagatgta ttcagttgga
     2341 tttgtaatac ttttttatgg ggattgatca aagggttata taatctcaat actttattag
     2401 aaaaaacaat ttatcaaagc tttgatttaa aagacttatt gaacgccgct ggtgttaatg
     2461 aactatttca aagtttgatt agtaaggtat tggctctttt ttgtgtcgtc acattaattt
     2521 atttaggctt gaaatttttt atgagtaaac acccaccgaa agtcaaaaat atcttggtta
     2581 atttatttat ggccatgatc ttgatttttg gtggtagttc tttaatcgat gaagggttaa
     2641 atatcagtaa aaacttttat ggagacgtca ctgcagcaaa taaagcagat aaaacgggtt
     2701 cacctgcttt tcaaattatt caaaataatg tgattgatgt cagtacatta ctagcgactg
     2761 acccgaataa agtagaatcc ttaccaacag ataaaagaaa tgctttaaca gcgaaaaatt
     2821 tcaaaacagc gaatatcaat gggattatta cacctgatca agctaagaaa atgtctgaat
     2881 caagtgatat tagtacagta caaaaagatc ggttaaaggc attacagtat cgactagagt
     2941 tagacgataa tgggaaagaa atccctgttc aaattagtga tgaaggttta gcaaaatatg
     3001 tgtatacgtc aggctatcgt cgatacatta gtagtccagg gattattttg gctggggaat
     3061 cttctttgtc ggtagcctat ttatttattt tgtttacaat cgttacttgc attattgagc
     3121 tagtatttaa aaaattctat ttagtaattg ccgcgtcaac ggactttgag acaggacaac
     3181 ggatgaaaag tgcgatagaa gatatttctc aatccttctt gttattggcg tttacgattt
     3241 tagaattacg aatctataca ttaatgcttt caggtatcgg tgatttgcat gcggtaggaa
     3301 agatcaatgg attcttgtat gttgttgctt taattgtgct aactattgcg ctattcaaag
     3361 gctctcaatc ggttacgaag attttcggtg ttgatacgag tctgaaaaac ggcagcaatt
     3421 ctcttcttag catgtttgcc ttaggaaata ttgcgaaaaa tgtgggtagc ggagctaaaa
     3481 accttgcaag tgctggtaag agtggtttaa gccatttaaa tgatattcgt aagaatggat
     3541 ttggccatcg aaaagatatt acccatgata acggggaaga acaatcagac gataaagcta
     3601 caactgtacc taatagtaag aagacagttt ccgaaatgtt cgggaaagcg aaaaatggtg
     3661 tttctaaagc cgcagaaggt cttggttacg tcaatgaacg tgggttaaaa ggaacagttg
     3721 aagatcttac agaaaaagcc aaagacacct tagaagagaa attagacggc acgagagcta
     3781 aagaatttgc tgaagctgtg aagaatccat ccgaagcact agaaaaacaa aaagaaaaag
     3841 cttcagcagt caaagaagga ctaaaagatg cgtatggtga aggacaagtc aatgcagcag
     3901 tgaaggcaaa tacaacaagt gaaaaagaaa atcaagaagg tgcagcgatt ccttcagcca
     3961 aagatcacca agatgagtta cattctaacg aaaattcgat cggagaaaaa gattcagaag
     4021 aaggacaaca tggtcagtta ccaaaaaccg atccagaaca atatcgttat aaatctaaca
     4081 ttaattctga aaacgaaaaa cgcatttcag atttaccaaa acgaaatcaa gaagtacaaa
     4141 cggatgagga ttccatcgat ctgccaaatg ataatggcat cgaagttgga actgaaaaat
     4201 caacgaaaat tggtttgcct aagacacaaa atttaccaat gaaagatagt cgtttgaatc
     4261 aattcaaaga gaatgatcaa agctttgttc atcaatcacc tgcaggtact gttcaaatga
     4321 aatcaagtcg tttgcctgaa aatggaaatc tgacaactgt ttcttacaat ttagctgaaa
     4381 gtggtgaaac agagaatgcg tccgttcaaa atattgtgac aaaagaagct gcacctaagc
     4441 caatcaataa agttcataat ggatacagac cagcaaacgt agcatttact gcaccgacaa
     4501 aaataaatgt gacaagagaa actaaaaaca ggaacaatga attgaaagaa caactattga
     4561 aggatatcaa accataaaag gcctatttaa gggccttttt ctttataaaa caagtttaaa
     4621 atggaaagga gaaaattgtg gaggatattt taatagctac gtttataact gcactgggag
     4681 catttttaac gatatttgtg gtgactatta aaggatttaa agatacggat agtttacaaa
     4741 aagatcatca gtctatttta gatggtcaaa aaacacatac cgaaagcatt attgtacacc
     4801 tttcagaagg attcacttat ttgaaagatg ctatgcccac attcacagta aaattaacgg
     4861 aaatatttca ttctgttgag caaatatcgt cagaattgaa agagcaaaga caacaaaaag
     4921 atcgtcaact tggctcactt aattttaggc aagaaacgat acaagaatca ttggagaatg
     4981 taaatcgatt aaacgatgaa atgcaacgag ttcatttgga aaatgaagaa ttacgcgcac
     5041 aaaattatga tttaattcaa aagaaaagaa atctggaaca aaaaattgtt gaattaaaag
     5101 aagaaaatcg tcaattgaat tatcaattac gacgaggaaa aaatcgttct cgtgatttag
     5161 gaaatgaatt agagttgtaa tatttagaaa aacgctgtga gaaatcacag tgttttttat
     5221 ttctaaaaat acaaaatata aggggtatgg ttatattatg gataaataca ttcatatgaa
     5281 aaatcctcca agatttagtg gggaatggaa gtttttcgcc tttttctttt tagatctatt
     5341 aacgcttggt ggctgctttt tttgggcgaa aatagtcaat aatcttgtcc acttaccact
     5401 cttttttgca ctcttgaatt atggattatg gcttgcgata gcgttagtga ttgtttggcg
     5461 accctataat aaccctggtc aacgccaaat ttttgcgatt atcgaatcta ttttctttac
     5521 ggataagaac cactatcatg cgttagatcc taaccgaaat tatcatggta cgtataagga
     5581 ggatgaataa atgcgagtca aaggacaaaa ttcagttgat aagaaatcca ttttgcagtt
     5641 tggaaaacca gaagagcgct ctatcttgga cttatttcca tataaatcct gtacagaaga
     5701 gggagtctta attactagag aagatcattt tcagcgttat tatcgtgtcg tttccactga
     5761 cgtggaggga ttaaatgaac aagagaaact agaacgaatg aatcagttga ccaccgtcat
     5821 gcgtacgtat gtgccaacaa ttaagttagt tagtttgacc acagagacgg atctaacgga
     5881 acaaatcgtg caaaagcggc agttattaga taaaaatcgt atggaacagt cactaggaag
     5941 aaatctacag cgattaagaa aatatgaaaa gaaattagta gaggagatcc aggaattaaa
     6001 acgggcagaa caagaacgtc ctgacttaag cttctttttc gttattgaag gaaagacaat
     6061 caaggattta caaactagaa atcgtcagtt aatgcgatct agtggcattt tagggttaaa
     6121 accactctac aaaaaagaat tgattaagat tctttatcga atgaacaaca tgaatgacga
     6181 ataaaaaaga tggaggaaat gattcatgct agatgtgata aaaacagctt tttcaacgca
     6241 aagtaatcaa aaggtgattg atccattaaa acaacttggt aatcgtgaaa tagagatttt
     6301 aaaatcaaaa ggttatgatt tagatttctt aaaacaagtg atgccacaag gtgggttgaa
     6361 ttttgatgaa gaatatgtga cctatggaaa tggttattca cgatgtgtga cagtttatca
     6421 ttacacaaaa aatcctactc ttttttggtt ggccaatctc atgaacaatc ctcatacagt
     6481 ggccacacta gatgttagca cagatgaaaa gcaaaaagtg attgatcaaa tcaaccgcgc
     6541 attagatgaa ttaaatgatc gatcagataa tgaacgaaaa gcgactgacc gaaataaaag
     6601 tgcggaagaa tacctggaac tagcggaata tgcccgaaaa ttgacttcta atggggaaat
     6661 ttctaaacaa gtcaaagtga gagtacatgt ttacggaaat acgatccaag aagtggatga
     6721 acgggcgcag gaactgatcg aaaatctaaa agggatggat tatcgtgcgg tgacttatct
     6781 gcataaaacc aaacgagatt ggcaatcgat gtttaccatg ttcgatgaac aagctgaatg
     6841 gtttggaaat atcaaagggc aatcacttcc atcgttaaac attggcggag gattcccgtt
     6901 ccaccaccaa acgctaaaag atccaggagg cacacaatat ggggaaacca tgacaggcgg
     6961 cccatttgtc tggaatgcca cgtataaaaa tagtgtacgt ttatctacca ataccttgct
     7021 tctaggaatg atgggagctg gtaaatcgac cgttcttaaa atgatcgcgg aagcccattt
     7081 agctgcagga gactttattt ggggctttga aaagggaaaa gactttattc ctttcttaaa
     7141 ggaatataac ggtattatgg tgcgtttaga cggatctgac gggatgatca atcctttaga
     7201 gatctttgcc acacggacgt atgatgaagc atccagttta tatgacgatg gttcagtcaa
     7261 agatttgaag atcaatgaag cagcgtccta tcaaacgcac ttagacaaag tagtctatca
     7321 ggtacagtta gtgagtcctc agctaaaagg aacgatgaaa gcagaattca agacctactt
     7381 gaatcgtttt tatgaagagt atggaacagt accacgcggt tttacttcct caaattcgcg
     7441 ttcaaatact gatacgcaag tcacagggaa agatccagaa gcttatccga cgttcaaaga
     7501 atttttagcc tttttaggac aactggaact accaggggct tcccaagaga aaaagaatcg
     7561 gaaagaagaa atggagtcta tcgtggaatc cttatgtgag acttatggca tgatttttga
     7621 tggtcatagt acgattcgac accttgacca acaacagttg gtcttttttg acattgactc
     7681 tattacaggc ctagatcgag gggtacaaca atgtcagatg tacatggcca tgacgttgat
     7741 ttggaatcag gcattgattc aaggacgaag acaacgacgc ttaattaaag aaaaacaaat
     7801 ggaagaacaa gacttcaaac gcttcttggc gatgattgat gagtgccata atgcgattaa
     7861 tccagatttc attgaaacgg taaattatgt cacaaacttc caacgagaaa tgcgtaaagt
     7921 agaagcaatg actgttttag ctacacaatc cccacaagaa atggtgccag aaaatatgtc
     7981 ttctgataac ttctcgaaac tgaagaaagt ctttgaattt tcttctacga agatctttat
     8041 gcgtatggat gaatctattt tacgtcatat agaaggatta gttggtaaat caatgacgaa
     8101 ctcagaatta gaatccattc ctcaacaaaa tcaaggagac gctgtgatca attttggctc
     8161 aaaagaaacc attcgtgttc attttagtcc gaacaaacgt caattaaaat tgtttgatgg
     8221 aggaaaataa tggaaaacca aaatgaaagt ttaatcaaac agtatgttaa gcgtcgtgca
     8281 aagcggcgct tatttttgtg ggtgtttggt acttctgcag gtttgattac tatattgatc
     8341 actgtgtttg tgaccctttt tctaatactt gcggcaggtt ctattgataa ttctgattca
     8401 agtagtggtg gcgaagcttt tacaggagaa tattcggaag gcttacccat ttataaagaa
     8461 atcaaaggaa gaggcccttt ttctgatgag attgcgcaat atgctgtagg tgcagcagtg
     8521 aagtataaat tattaccaag tgtgatcctt tcgcaatacg gttatgaatc agcgtttggt
     8581 acgtcggcgt ccgcaagaaa tgatctcaat tattttggga ttacgtggtt tgatgggtgt
     8641 ctgtttccta aaggaactgc gcgtggaatt ggtggcattg aaggtggctg gtacatgaag
     8701 tttcccaact caaaagctgc cttctcctat tacggcttta tggtagccac acaatcaaat
     8761 tttaatgctt gtgtcggaaa taaaagccct ggcgcaagct tattgatctt aggacgagga
     8821 gggtatgccg ccgcaggaat tactgaagac agtgcctatt ataaaaattg tatgtctatt
     8881 attaatacaa ataaattgat ggagtacgat gagtttgcga ttaaacactg gagagaaggc
     8941 ggcggaactg gtggtacgat tacaggaagc tggggcaatc cattcccagg aagtagctta
     9001 gataaaaatt cgttcagtgg aggacaactt tttgggaaaa atccaggagg agaattccga
     9061 cctaacgggt tccatgatgg actagacttt ggttcagtcg atcatccagg aagtgagatt
     9121 catgcggttc atggcggaaa agtcgtgtac gttggcaatc ctggtatttc aggtttgggt
     9181 gcgtgtgtga tcgtgattaa ttacgatggc ttgaatatgg tgtatcaaga atttgcaaat
     9241 agtacaggaa attctcgagt gaaagtaggc gatcaagtga aagttggtca agtaattggg
     9301 atacgagata cagcacactt gcatttagga tttactcgaa tggattggcg tcaagcacaa
     9361 ggacatgcat tcattgatga tggtacatgg atcgatcc
//
LOCUS       DAMYXM010000090         9269 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.093, whole genome shotgun sequence.
ACCESSION   DAMYXM010000090 DAMYXM010000000
VERSION     DAMYXM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.093"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..668)
                     /locus_tag="PNS21_002481"
     CDS             complement(<1..668)
                     /locus_tag="PNS21_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDI5160140.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            802..1089
                     /locus_tag="PNS21_002482"
     CDS             802..1089
                     /locus_tag="PNS21_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160141.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1505..3043
                     /locus_tag="PNS21_002483"
     CDS             1505..3043
                     /locus_tag="PNS21_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDI5160142.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3040..4005
                     /locus_tag="PNS21_002484"
     CDS             3040..4005
                     /locus_tag="PNS21_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5160143.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3992..5314
                     /locus_tag="PNS21_002485"
     CDS             3992..5314
                     /locus_tag="PNS21_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI5160144.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5377..5481
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5555..6298
                     /locus_tag="PNS21_002486"
     CDS             5555..6298
                     /locus_tag="PNS21_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5160145.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6313..7305
                     /locus_tag="PNS21_002487"
     CDS             6313..7305
                     /locus_tag="PNS21_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI5160146.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7317..8054
                     /locus_tag="PNS21_002488"
     CDS             7317..8054
                     /locus_tag="PNS21_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI5160147.1"
                     /translation="MSIQISHLNVVIENLKVLEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8044..8766
                     /locus_tag="PNS21_002489"
     CDS             8044..8766
                     /locus_tag="PNS21_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDI5160148.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8850..>9269)
                     /locus_tag="PNS21_002490"
     CDS             complement(8850..>9269)
                     /locus_tag="PNS21_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDI5160149.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3028 a         1603 c         1962 g         2676 t
ORIGIN      
        1 cagtgctcta actgttcaag aggatcaagt ggttcaaatg tttctgtagt ctgttttgct
       61 tccaattggg gcaaaacaga tccaatttcc agatacttca ctttattcaa tgtataagat
      121 agaatagaac agctaagtaa tgtatacaga aaatgtgtga gatccaaata aagcaaggag
      181 acaactaaaa taccaaaagt aatcaagaaa agacttttgc caatcttaat atgaaaatat
      241 ttttcaatgg cttgataaat gatatctgtt ccgcctgtgc ttccgccgaa tcgaaagacg
      301 agaccgcaac caatgccaga aagaatacct gtcatcaaac tattgatcag tagactatct
      361 acagcaaaat gtatatgaag gctctcccat aaatgtaaaa agaaaactag cgcaaatgtt
      421 ccgacgatcg tccggagaaa agcgtgtttt ccaaaaagaa acaatgagat aagcaataaa
      481 ggtaaattaa aaagcagtga agtgacagaa ggagcgaatc caaaaagatt atgcaaaagc
      541 aaagcaatac cagtaacccc accatctgcc agcttagatg gaatattgat tcctgtcaca
      601 gctaatccgt agattcctgc gcctaaaatg attccgcaca attctgtaaa taaatttaat
      661 tttttcattt ctagcctctt ttcttttgaa aattttcagc aacttcttta ttatactgac
      721 ttatactctt acttcaagag gaaaatgaaa aaaaattatt tttggctgtt tctttttatt
      781 tatcaaataa ggagtggaaa aatgcgagca gcagacttat tgacaattct tacaaaatca
      841 cccaaaaaaa cagagtatca aggagttttt ttggctcaag aacaaaaact ggtgcctgtg
      901 acaatggtcc agataaataa agcaaatcaa tttatctttt tatttgaaca cggcaaagca
      961 ccacttgcta tgaaagacat ctttcttttt ttgatgaaga accgtgagaa agctgtttat
     1021 tatcagtcaa aagaagaact cattcctctt tatggagtga aagagataga aaataaagtg
     1081 gtgatataag taaaaataag aaaacaagac gtttatgatg tcaaaacaac cattttttac
     1141 atttttggca aaaaagtgtc atgatacagt ataatattcg ttgaacaggc caataaaggt
     1201 gttctccccc acgagatttc tactttattg gctgtttctt tttattaaaa taaaagttgt
     1261 gtgtgtaaaa aagatatagc tgattccaag tgacaatttc gtatagattt ttatggtatt
     1321 cgtagtgaaa aaacgagaac caaaaagttg atccgacttt gttgcttgga ttcttttttt
     1381 gtcagcatat ttagaggtat ttttttcttt tttttgttcg tgctgtaagg ttaaggcaag
     1441 acaagttagt tgggaaaaag gaaagaaatg tttatactta aagaataaca gaaaggaagg
     1501 tcgcatgaat aattttatac aaacattaag tgaaagaaaa ggcgagttgc tggaagctgc
     1561 atttcaacat ctgtccattt ctctcatttc tttgctgatt gcagcactga ttgctattcc
     1621 tttagcgatt tgggcagcca atcataaaaa aatggcagaa gtcttattgc agatcacggg
     1681 tgtgctccaa acgattcctt cacttgcctt attaggttta cttatcccat ttgtagggat
     1741 cggaactgtt ccagcattga ttgcacttgt tatttacgct ttgcttccga ttttccaaaa
     1801 cacgtatatt ggtctagctg aaattgaccc gtctattgaa gaagcagcgg tagcatttgg
     1861 tatgtccaga atgagacgct tgctgaaagt agagcttccc atcgcattac ctgtcattat
     1921 ctcaggtatc cgaactgcct tagttttaat catcggtacg gctactttgg cagcattgat
     1981 cggagcaggt ggattaggga catttatttt gttaggtatt gaccgaaata cacctgtttt
     2041 gacattgatt ggtgcgatca gttctgcctt gcttgcaatt atattcagcg gactgattcg
     2101 ctggttgcag cataagaaac ctcgatattc catcattact ttagcagcag tggtgtgttt
     2161 atttggcggc gtttctttat atcagtctgc cttgtttaaa aatgaaacca tcacgattgc
     2221 tggaaaatta ggagcggaac ctgaaatcct gatccaaatg tataaagaat tgattgaaga
     2281 caacacaaaa acagaagttg aattaaaacc gaattttggc aagacgagtt ttttgttcaa
     2341 tgcactggat caaggtcaaa tcgatatcta tcctgagttc acaggtactg tcctagaaag
     2401 cttggtgaag gtacctgaag caatgaaaag tgctgatttg acaaaagaaa aaacatatga
     2461 cgaagcagaa gaattattga ataagcaatt cgatatgaca ttattaaaac caatggctta
     2521 ccaaaatacg tatgccttgg cagtcaagag atcttttgcg aacgaacatc aacttaaaaa
     2581 gatttccgat ttggcagcta tctctgatca gatcaaagcg ggattcacac tggaatttat
     2641 tgatcgttca gatggatata aaggaatcca agaagcatat ggcttgaatt tttcaagtgt
     2701 acaaagcatg gagccaagtt tgcgttacca agcaatcaat aatggagatg tcaatattgt
     2761 ggatgcctat tccacagata gccagctcaa ggagtacgat ctagtgactc tagaagatga
     2821 tcgagggtta ttcccagctt atcaaggcgc accgctgatg aaaaaagatt ttgctaaaga
     2881 acatcctgaa atcgttaaag cactgaataa attaagcgga aaaataacag aggatcaaat
     2941 gattgatatg aattaccaag tcaatgtcga aaagaaaaag ccgacagaag tagcacgaat
     3001 cttcctgaaa aatgaaggat tagtaaagga ggaagatcag tgactaaatt aatcgagttc
     3061 aaacatgtgc aaaaaaagta tgatggaaag tatgtcatcg atgacctaaa tctggcaatc
     3121 aaaaaaggag aaatctttgt tttggttggt ccttccggta gtggaaaaac aactactttg
     3181 aaaatgatca atggattatc taaaccatca gcaggagaca tctacttcaa aggaaaatcg
     3241 ttaaatgaat ataatctgca gaaaatgcgg tggaatatgg gatatgtgct gcagcaaatc
     3301 gctttgttcc cgaccatgac agtcaagcaa aatattgaag tcatccctga aatgttaggt
     3361 tgggaaaagc agaaaagagc agaccgtgta gatgaacttc ttcaaaaagt tggcttatct
     3421 cctgatattt atcgagatcg tatgcctaga gagttgtctg gaggagaaca gcaacggatc
     3481 gggatcatcc gagctattgc tgcaagccca gatgtgattt tgatggacga gccatttagc
     3541 gcattagatc cgatttcaag aaactcatta caagaattgg ttttgtcctt acacgaagaa
     3601 ttgggcacga ccatcgtttt cgttacgcac aatatggaag aagcaatcaa gctaggtgat
     3661 cgtattgcat ttatgaagga tggagaaatt atccaatgtg acacaccaga gcagttattg
     3721 atgaatccta aaaacgacta cgttcggcat ttctttgatg aaccgaaaca gacgaaagag
     3781 tggcgagtag aagatttagt cgtcaatgga tatttcttaa atgaaatacc agaaaatgct
     3841 cgtcaacaag tttgctttga tacgcctatg aaagaagtat attcgcttct ttcggtgtat
     3901 ccaagcattg tgatagtaga aaagcaaaga agtattgggt cgctgacaag taaagaaatc
     3961 tttgcttttt taagtcgaga ggaggaagga aatgaaaaca tatgatgcaa tcatcatcgg
     4021 ttcaggtgtg agcggcttaa gtgctgctta tggattgaaa gaagcaggta aaacagtact
     4081 agtggtcgaa gaagatctat ggggcgggac ttgtcctaac cgtggatgtg atcctaaaaa
     4141 agtcttgtta agtgcagtag aggcacgtaa tcgggtaaaa caattgagtg gaaaaggttt
     4201 taacgagatc ccaactgcca actgggaaga acttcaaaaa ttcaaacgaa catttacgga
     4261 tccagtccca gaaagccgaa aaaaacaatt agcagaagct gaaatcgatc atctttcagg
     4321 aaccgctcgt tttttggatg attcatctat tgaagtcaac gaagaagtct tccatgcgga
     4381 ttacttagtt ttggcaaccg ggcaaagacc tacgatctta ccagtggagg gaaaggaata
     4441 cttgaaaact agtgcagact ttttatcatt gcctgtactg cctaaagaaa tcatttttat
     4501 cggtggtggt tatattgctt ttgagctagc tacgattgcc aatgctgcag ggagcaaagt
     4561 aaccattgtc catcataatc aacggccatt aaaagaattt gaggcgtctt tggtagagga
     4621 agccgtccat caaatggaag catctggcat tcagtttgct tttggtgttg aaacccaaaa
     4681 gatcatttca gaaggtactc gctatcgttt agtcggaaaa gagacggaat tagtagcaga
     4741 tatgattttt tgtgcgactg ggagacagcc gaatacagaa tctttagcac ttgagcaagc
     4801 gaatatcgtc tttgataaac acggaattgc agtcaatgat tatctgcaaa cgagcaatcc
     4861 aaaaattttt gcgtgtggag atattgtttc aaggaaaacc ccaaaattga caccagtcgc
     4921 cacttttgaa ggaaattatg tagctaagcg gataacggat gcaacaagcg aacctatcaa
     4981 atatccgatt atacctacga tcgtctacgc cagtcctaaa cttgctgaag taggagttac
     5041 aaaaagtcac gcctcttcgt cagatcaagt ggtagagatg gatcttacca gttggttcac
     5101 ctatcatcgt gtcaatgaac cagttgctaa ggctgaattg acctttgacc agcaaaatta
     5161 tttgattggt gctgcagtga ttagcgagca agcagacgaa ttgatcgatg atttgacact
     5221 cgtcatcaat caaaaattga ctaaaaaaga gttggatagc tatatcatgg ggtatccgac
     5281 attggcaagt gatctctcct atttattgaa ataatcagca tgataactaa agcgcttgtt
     5341 gctttttcct tcttgtgcta cactttaaaa aatgaatacc tgtttctggg gatgtcgtag
     5401 acgacatgag agtaagttgt gaacttagac ccattgaacc tgtcagttaa tactggtgaa
     5461 gggagaaaca tagcagacgc ttgaagcggg ttactatgtc gtgcccattt caagcgtttt
     5521 ttgttattca tattagaaga aaggaggtaa caaaatgaag aaaaatcttt atccagcagt
     5581 cagtatggca ttcattttaa ttatttggca gattgttgtc atagtttgga aaatacctgc
     5641 ctttatttta cccggacctg ctgctgtatt ttctgcatta gcggcagatg cacaagtatt
     5701 actttcccat tctgtcacga cattaaatga agcagtgctt ggtttggtaa tagctagcgt
     5761 tctttctttt attactgcct tggcaatgga ttattggaag tttttagaaa tgtcttttta
     5821 tccgttacta gtgatttcac aaatgttgcc aatcatggtg ttagggccat tacttaccct
     5881 atggtttggt ttcggtatgc ttccaaaagt gatccttgtt gtcttgataa gctattttcc
     5941 gatcgtagtt tctttttcgg atacattgag gaaagtatca aaagagcaaa tcatcttttt
     6001 gaaaacgatg ggggcagata cagggaagat ttatcgaata tataaaatac cagcgggttt
     6061 atccggcttc ttttccggtt taaaagtggc tgctacttat tgtgtcagcg gagcagtcgt
     6121 tggagaatgg ctaagcgctc aagcaggctt agggtattac atgatacgcg taaaaaacag
     6181 ctaccagatc gataaagttt ttgctgcgat catttgtgtc attctattga gtttattgct
     6241 gaatggcgct tgttcattat tgaaaaaagg gtattatcgt atgctttata ttttttaaga
     6301 gaaggggatc aaatgaaaaa attatggctt ttattaccgc tactgttgct tttatccgct
     6361 tgtggcactg caaaagaaac aagtagcaaa caagaaataa aggatttgaa aaaagtgaca
     6421 cttgttttgg attatgtacc gaatactaat catactggca tctacctagc aaaagaaaaa
     6481 ggctattata aagaggctgg tttaaatgtc caaatcatcg aaccaggaga taattcgaca
     6541 agtattggtc tagtaggtgc ggacaaagca caatttggtg taagttatca agaagatgtc
     6601 acttatgctc acgcagatgg acagaatata ccagtcaaag caattgctac agtgatcaaa
     6661 cataatactt ctggattcgc caccctatca gacagtaaca tccattcacc aaaagatttt
     6721 gaaggaaaaa catatgcagg ttggcaaagt ccaagtgaag aagctgtctt aaaagctgtg
     6781 atggaaaaag acggcgggga cttttcaaaa ctgacaatgg taggaagcaa tggagaaggg
     6841 ccagaaagct taggaaaatc ctcagatatc caatggtact ttgaaggttg ggatatgatc
     6901 aaagcaaaag aagcgggtat tgaactaaac tatatcccat tgaaagaatt agatgaacgg
     6961 ttagattact atactccggt aattattacg aatgatcaac tgatcaaaag tgatccagaa
     7021 ttggtacagt cttttatgga tgccacgaaa aaaggctatc aggaagctat taaggatcca
     7081 aatgacagtg ctaaactgtt gcaaaaatat gcaaaagaaa atgatcggac gtttttagaa
     7141 gaatcacaag cattcttgtc aaaaaattat acggatgatc ctgagaattg gggattgatg
     7201 gaagaaaaag tttggcgcaa ttatacagct tttatgcagg aaaacggatt gatcaagcaa
     7261 gatgtacctt cgcaagaact attcactaat cagtttataa aataggagtg atagatatgt
     7321 caatccaaat cagccacttg aatgtagtca ttgaaaacct gaaagtatta gaagacatcc
     7381 atttcactat ccctaaagat acctttacat cgattgtcgc gccaagcgga gccggtaagt
     7441 caacattgct gaaaacttta acaggagtcc agcctatgac ttctggaacg atcaaagtag
     7501 acgatcaaaa agtaactggt ctccatacag catttagtta tatgccgcaa gaagatatgt
     7561 tacttccttg gctgagcgtc tatcaaaacg ttacgttata tcaaaaaatc aatcatctaa
     7621 caatcaatga agaacaagta agggattatc tggatatctt tgggttaaac ggctatgaac
     7681 attttttgcc ggaacaatta tctggcggga tgaagcagcg aacggccttg ttacgaacaa
     7741 tcatgaatcc ttcttcttac ttgcttcttg atgaaccatt tggagcgtta gatgctatga
     7801 ccagagggca gatgcaagac tggatgttga agctgcctaa aaaagcaaag cggactactt
     7861 tacttgtgac gcatgatatc gaagaagcaa tttatttatc agacaggatt cttgtgcttt
     7921 ccgcccgtcc ggcacatgtg attgctgaga ttcaagtgcc tgaaaaacga cggtcacgtg
     7981 aatggctact gcagcaatca gagttgaagc aaacgattta tcagttattg gcaggtgaat
     8041 tagatgttaa gtgatgctca ttgtcattta gatgggagtc agagtttagc actccttcag
     8101 caggaacaca cgattttgac gatcatcaat tgtgattcgc cagaagaatg gaaggaaaac
     8161 agacagcttg cagcaagtaa gactcaagcc ttaagttacg gtatccatcc ttggaaagca
     8221 gatagctata cgtttgaaca agttgagcct tttttgaaaa aagcaaggat catcggtgaa
     8281 atagggttag ataatatttg gacgaatgta ccgatgacta cacaaaaaaa agtatttgag
     8341 cgacagcttg cttttgcagc cataaatgag aaacctgttg tactgcacac aaaaggatgc
     8401 gaaaaagaga tcttaaatta tatccaagct taccctaatc actatttgat ccattggtat
     8461 tcttctttgg actatcaaca agactatatt gatttaggct gttatttcag cattggtctt
     8521 gatttgaaaa agaatccagc agtctggcaa ctagctaaag cagtcccaat cgaccactta
     8581 cttattgaaa cggatggact acatgcagca gagtgggctt taggggggat tttttcagaa
     8641 aaagattatg tctcactgct tgaagaacag ttaaagacaa tcgctgagat caaagagcta
     8701 tcagttgaag aagttaaaaa aaatacttat caaaacttgg aacgtttcat tagattaggt
     8761 aaataaaaaa gagatgtgac acaactagtg tcgcacctct ttttggcttt ctttgtataa
     8821 aagtaaactg tttgattaat aaatgaatct tagtaccaac cgtttgcttc ccagaatgct
     8881 ttcgccgctt cccaagaacc ataacgtgaa gtaacatatt gttctgctac tctttcttgg
     8941 tttgcagcag agtagtcacc atttaagtaa gaagaatcta attggtaacg tccgatgtaa
     9001 cgtccgttcg ttgctgtgta agaaccgctt gattcttttt gtgcgatcca ttcttttgca
     9061 gagcttgtgc ttgctggagt agcagcagtt tctgttacag caggagcagg ttgtgtttca
     9121 gcaacaggcg ctgcttgttt ttccgtttga accgtttctt gtacaacagg ttgttcagct
     9181 tggactggtg cttcttgaac ggctgcttga ggttcagcag ctgcttgtgc agagccttct
     9241 gttgggattg ttaattgttg tcctgagta
//
LOCUS       DAMYXM010000091         8863 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.094, whole genome shotgun sequence.
ACCESSION   DAMYXM010000091 DAMYXM010000000
VERSION     DAMYXM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.094"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..427
                     /locus_tag="PNS21_002491"
     CDS             <1..427
                     /locus_tag="PNS21_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160150.1"
                     /translation="RYSKNKKAEKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFME
                     RSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTY
                     LYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(553..1229)
                     /gene="nth"
                     /locus_tag="PNS21_002492"
                     /pseudo
     CDS             complement(553..1229)
                     /gene="nth"
                     /locus_tag="PNS21_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(1236..1934)
                     /locus_tag="PNS21_002493"
     CDS             complement(1236..1934)
                     /locus_tag="PNS21_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDI5160151.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2202..3386
                     /locus_tag="PNS21_002494"
     CDS             2202..3386
                     /locus_tag="PNS21_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HDI5160152.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3392..3835)
                     /locus_tag="PNS21_002495"
     CDS             complement(3392..3835)
                     /locus_tag="PNS21_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDI5160153.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4061..4348)
                     /locus_tag="PNS21_002496"
     CDS             complement(4061..4348)
                     /locus_tag="PNS21_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160154.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4519..5871)
                     /locus_tag="PNS21_002497"
     CDS             complement(4519..5871)
                     /locus_tag="PNS21_002497"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HDI5160155.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6144..6590)
                     /locus_tag="PNS21_002498"
     CDS             complement(6144..6590)
                     /locus_tag="PNS21_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDI5160156.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6826..7752)
                     /gene="cysK"
                     /locus_tag="PNS21_002499"
     CDS             complement(6826..7752)
                     /gene="cysK"
                     /locus_tag="PNS21_002499"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDI5160157.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7892..8779)
                     /locus_tag="PNS21_002500"
     CDS             complement(7892..8779)
                     /locus_tag="PNS21_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDI5160158.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2421 a         1801 c         1456 g         3185 t
ORIGIN      
        1 tcgttattcc aagaataaaa aagctgaaaa attttgttta ttattaaata aatttacttt
       61 aattgctcct atccttgcat ttattatttt ttctgtactt ttaagcacta ctcttaaagg
      121 aaaatttatg gaaagaagct ctcatgcaat gattctcact tttttatggt tgttgttcac
      181 cagaatctat atttttctga tgagcctgaa accgcccaaa tccattagtt tatgtttagt
      241 tataaatggt atttttttat tatcacttat catttttatt acacctttag atagatacgt
      301 cacttattta tataatcctt tggaatactg gacttatttt ataggtatat tggaaggcat
      361 tattttctac atcgggtatt ttcccaataa aaataacaac ttttattttt atcgcaataa
      421 attgtaataa gttaccatcc tagctactga gtatgtacat ttactttttc atgcaaaaaa
      481 gataaacgaa acagcttttc tccataataa caagataatt catacattta tttgcaatta
      541 aagaaaccac gtctacaggc gtggtttctt ttttagcgcc ttttctttta gacgcatccg
      601 atttttccca tcttgacata ttgatagcaa aggacatacc tcgcacttgg gatttcttgc
      661 cgtgcaatga tatctaccaa aaaaatcagt gtatgatgtg tctttaccca taattcttgt
      721 ggtactttcc gcatcagtgt ttcttctact tccatgaccg tagcatctag tttgcagatc
      781 ctcaggcgtt ttgagacacg ttccacgtgg gtatctactg cgattgctgg tatcccgaat
      841 gcatccccca atacgacatt tgctgtcttt ctgcctacac caggaagaga catcaattct
      901 tctcttgacg ttggcacttg cccatcgaaa cgttcaatca gctgctgtgc acacgctttg
      961 atgttttttg ctttatttcg atacaaacca atcgttttta ttttaagaat gatttcatcg
     1021 attgaagctt ctgctaacgc atcgggtgtc ggaaaagagg caaacaaatc tggcgttgct
     1081 ttattcactg atacatctgt cgcctgggca cttaagatca ctgcaatcaa taactcaaat
     1141 ggattattgt gcttgagttc tccatgtgct tcgggaaaca ttccatacat cgtttctaga
     1201 gcttccattg ttttttgttt gcttaacata ttccgctact cctccaacca atttttcagt
     1261 gagactttcg gcaattcttc ttgttgagct gactgttcta cttctctttg aagaagctgc
     1321 tgttttcttc gtttttggtc ttcttccact tgttgttttg tacgtagatt ttttctttcc
     1381 cacgatagca aaatgcggtc aacgtaatta aaactataag cctgatttaa tacggcttcc
     1441 cgcagtgcta gcttcaaaat ttccggctga taatggtctt cttctagcca ttgtccgatc
     1501 cgctggaact cgatcgaaga aagcgggcgt ccaaattctt gttcaaataa ttgatacact
     1561 gactggattt ctttttcttg tgttttttct tcgtattttt tttcttgtgt ttttaaatac
     1621 tcacctaata gatcatagat ggggtaaaga ttatatcgat ctgctttttt accgttgaca
     1681 atcgtcgttt ctatcttgat aaatccattt gttacgagac gatccagtat ttgaaaaatc
     1741 tgatcttgtt tgattcccat atccatagca atcatctgca aatcagggaa agtatctcct
     1801 tcaagctgtg ccatatgcaa ctgtagaaca aataaaaatt cttcagattg tagaccgatc
     1861 ctatgataat ttttaagtaa aagattagat atgactgttt gtccagcgct taagtaattt
     1921 tctaaattca acatgatttc tcctccgctc aatcacagta tacgtaaaaa aaacatggga
     1981 agcaagaaca ggtttctttt ctatttactt cactgatcgg tcagatttac aatgtcgtct
     2041 acttgatttt tgtttgttca tgtttttgat gcgtatcttc acttttcgga ataaataaat
     2101 taagtagatt tcgctgttcc ttagaatgtt gacaaggaaa caaaattact atacactatt
     2161 tttgtttcca agttaacatt attgcataaa ggagttttta catgtacagt ttcacaaata
     2221 aacattctaa agtttttgga tacgtctctg tcttattcac tggattagga tttactatcg
     2281 tcagtcctgt acttccgttt ttaacgctgc cttattctca ctccatacat cagcaagctt
     2341 tctatatcac tttgctcatg tctgtttatg ctctagctgc cttcttatct gcccccattc
     2401 tcggtagctt aagtgatcat tttgggcgtc atccgatcct tattatcagt ttattaggat
     2461 caagtatcgg atatttgctt tttggtttag gaaattcgat atggatgttg ttccttggtc
     2521 gtatcattga aggattgacg gcaggagaaa tctccacctt atatgcttac tttgccgaca
     2581 tcacggaacc taatgaaaga accaaagttt ttggatggat gggtgcttta gtcggtatag
     2641 gaacaacgct gggtcctatt atagggggtc tattagctga gttggggaat tctgttccta
     2701 tatttattgg agcactcttt actttcttaa atgccgttta tggctacaca tttatgctag
     2761 aaagccttcc aataaaaaaa cgttcagtag atttaagttt ctcccatgta agaccttttc
     2821 accaattaca acaattattc aagatttcat ccgttattcc tttattgaca gcaggttttg
     2881 ctgtttggtt ggcagctggc tctcttcaat ctatcttttc tcaattttcg attgacgcct
     2941 ttcagtggaa ggctgggttg gttggtttaa gcttctcttt gattggaatc ttggatactg
     3001 tctcgcaatt gttcattatg cctcgattgc tcaaaaaatt ttcagaacag cagatcacaa
     3061 gaatagggat gttcagtgaa atcctcgcct atctattcat cacattatct ggaattcttc
     3121 tactgccgat tttgttttta tttgggatta tctgttatgg tttcggtgat tctattttta
     3181 ctcctgtatt caatggccaa ctatccaatt ctgtctcaga taaccagcaa ggtcttgtaa
     3241 tgggcggtac ccaaagtata caatctctat cgcgcgtcat cggcccgcta atcgcaggac
     3301 agctttatgc tgcagcacca tttcttccga cggtattcgg atttttgctt atgatttttg
     3361 cctattatca ctacaataaa aaatagtagc ttcatttgct acttcgtata aattctctgt
     3421 atcgaccgat aaaacgaaat actgtgtcaa attcttcttt tgacatattt tcaaatacag
     3481 tagcatctcg ctgtacccat ttttgatgga gttgctcgtg tctattcctt attttttgtc
     3541 cgattggcgt caattgaaaa tagatttctt ttttattctc ttcgctttga taagtgttta
     3601 tgatccctct agctatcagt ttttttgtta gcttactgat ggctcctcta gtcatgtaac
     3661 tagcattcgc taattttgtc acgttaggat cagggatttt cccgatatgt tcaatcactt
     3721 ctagttcatt tagagtaaat cctgcaaatt ctttttccat ttctggttta ttacgatgaa
     3781 taatctcatt agctaaatcc agatattctt ctattagcag ttgtctttta tccatttcta
     3841 caccttcttt ttctttcatt atattgtttt tttgtttcct attcaacaaa gataaaaata
     3901 ttttgtagga aaacccaatc tgaaaccaag aaaaaaaggg gttgacaaag cttgtcataa
     3961 cccctttttc tgattaaaag atagtaaaga gacagtcatc ggtaattgtg atgatttttt
     4021 gtcatcactt aaggatgagc ggccttttca aaaactgtct ttaaagatca tcatcagttt
     4081 ctagcacata tggctgacct tcatatgtgt actctgtatt caatgtgccg tcattttcat
     4141 ctgagtagat gcgtctactg aaaggaaaac gatgagaaac attatctttg gcgtcatttg
     4201 ctggcatcgt gtaatattcc tcttgcgttt cattcaactg agagagtact tgatctgcca
     4261 actcatcaat attgatcgtt aatttcttcg aagcactttt ataatcttct aaaaatgcat
     4321 cttgttggac aaaatattct ttattcatct tctttctcac ctttcttttc aaaatcaaat
     4381 aaagctgctt gttcttttat gtaacagctt tatctttgct gcttattgtg acaaaaaatt
     4441 cgaagaaaga caactaattg tacttttcac aaaaaaagac agagggtcgt gacaaaattg
     4501 tgtcacaacc ctttttcttt attgtgcagt ttcgatttcg cctagcgctg cttgtgcaag
     4561 taggttcaag taattccatg ggcggtcaaa atgtggttgg aagaagaaat ctgaaagtgc
     4621 gagatcttct acagtcattt tattttgaac agctaaagac atggtatttg ctgattgtgt
     4681 aatgtcgtat ttggacatga actgagcacc tacgatacga ttcgttcctt tttcatagac
     4741 tagctccatc attacttttt cagtagttgg catgaactct ggacgataat tatcttcaaa
     4801 tacagtcgct tggacttcta gatcgttcaa agttgcgctt tctttggtca cacctgtcga
     4861 accgatcgtc cagccaaata ggtaaagtcc tgaagttcct tgagtaccac gatacgccat
     4921 tttatgatct gtcaagttat ttcccacaag caaaccttga cgaacggcat ttgttgccaa
     4981 aggaatatag ttttgcgtgc cacttggatt gtagttgact actgcagagt ctccagctgc
     5041 aaaaatgtct ggattacttg tctgcatgta ttcattcact ttgattgccc cgtttggcag
     5101 tgtatctact tttccttcga tcagttttgt atttggacgg aagcccacac ataaaatgac
     5161 catatctgta tcaaatgtat ctgttggtgt agctacttgt ttcacttttc cttcttcatc
     5221 tgtgataaat tcagtaacat tttcgcctag cgcaagagta acaccgcggt ctgtcaattc
     5281 tttttctaag atatctgtga atggtttatc tagatattta ttcaagatgc gatctaatcc
     5341 atcaatcaat gtcacttgtt ttccagattc gacaaaagct tctactaatt caataccgat
     5401 atatccacca ccaacaatta ctacacgttt tgcttctttc gcttgtgcaa taatttcatt
     5461 tgcttgattg tagtttttgc ataggagtac atttttcgca tcgattccac taatcggtgg
     5521 gataataggc catgaaccag ttgtcatcac taatttatca taagaaaccg tttctgtttc
     5581 tcctgttttc agatcttttg ccgttactgt tttattttct gtatcgacgt ctgttacatc
     5641 gtgttccatt ttaactgttg ctcctaatga agataattct tcaggatttg agtagaacaa
     5701 gcctgctggg tcctttacta ctccgccgac ataaagtgcg ataccgcaag ataagaaaga
     5761 aatattgtca tttctttcat atactgtcac ttctgcttct ggatgatttt ttaaaatact
     5821 ttttacagct gcagtacctg catgagtaca gccaattact actactttca tttatggtcc
     5881 ctccgattaa cattttgtcc ttcgacaaaa ctactataac agaaataaaa cggatacaac
     5941 agtttaatgc ttgtgaattc acaaaggttt gtttgtagtt tcattccttt tcaagcataa
     6001 gaacccaata tctacaagat tcttatttta ataatattca cttttatatc taagattgtg
     6061 aataaaagac aaaaaaataa aaaagctagc gtttttttta cacagctagc tttttttagg
     6121 tataacactt gtagaaactg tttttacatt tgcgcctttt ttgctgactc tttacaatcc
     6181 ggacagagtc catacaattc caaccgatgt tcattaatct caaaatcagt cagtctgctt
     6241 gcagccattt ctatatcttc aagccctggg taatgaaaat caacaatctt accgcagttt
     6301 tcacaaatta catgataatg acgctttgaa ctgaaatcaa atcggctaga agcatcgccg
     6361 tacttcattt cttgaacaaa tccaatcgaa gtaaataagc gaaggttatt gtaaacagtc
     6421 gcaacactca tatttggaaa ccgatgttca agtgctttat aaatctcatc agccgttgga
     6481 tgggaatgat gttcaatcaa atattctaaa accgcgtacc gttgaggtgt gattcgaatg
     6541 ttctcttttt ttaacttttc gataacctct tcgactaact tattttccat gtaaacaccc
     6601 cacgttgtta ttctcgtctt atcatactat ttttacattt taaaatccac ttaacatgaa
     6661 aagaaaattc aattttattg tattttaatg aaaaaagacg taaaacagtc gcgttcacta
     6721 ttctacgtct ctattcaaat aatttttctc atcataaatt tttgtgtgtt cttttcatct
     6781 ttaaaagttt tttacataaa cctatctgga gaaaaaccaa gtgccttata tctcttggta
     6841 aagggctgta gataaataac gttcaccatt gtcgggaacc acagctaata ccttcttgcc
     6901 ttttcctaat tcttttgcga cttttagcgc ggcagcaatt gcagcgccag atgagatgcc
     6961 tactaaaatc ccttcttttc gcccaacttc tctagctgtt tccatcgctt cgtctccact
     7021 aatagaaagg actttatcat acacagaagt atccagtgtt ttaggaacaa aacctgtacc
     7081 gattccttgg attttgtgag gtcccgcttc tttcccttct aaaatagcag attctgctgg
     7141 ttctaccccg attaattcga ttttcggata cacacgtttt aattctcggc cagcgccagt
     7201 gatcgttcct cctgtaccga tacctgcaac aaacgcgtcc aagccattta ctccaaatgc
     7261 ttgatgtatc tcaggaccag tcgtcttctc atgcacaaga ggatttgctt cattttcaaa
     7321 ttgtaatggt aaaaagtagc cgttttcttt tgccaaacgt tctgcttctc tgattgatcc
     7381 agaaatacca tccgcaccag gagtcaaaat caactctgcg ccgtaccctt tcatcaataa
     7441 acgtctttcg atactcatcg tctcaggcat cacgatgatt actttatatc cttttgcaac
     7501 tcctaccatc gacaatccga ttcctgtatt tccagaagta ggttcaatga tagtatcacc
     7561 tggtttcagt agaccatcgt gttctgcttt ttctatcata cttaaagcaa tacgatcttt
     7621 tacacttcct cctggattaa aaaactcaag ctttacaaaa acatctgctg aatcttctgg
     7681 tacgatttta tttaatttga cgattggcgt tttgccaatc aattcggtga ctgagttata
     7741 gatagctgtc atcttcaatt cctccttgtc tttgctcttc ttacttatca taatacaatc
     7801 tgttcattta tggcgagtga ttcgtctttg actagcaaaa aaaagacaaa gatataccat
     7861 atatggtaat ttctttgcct ttttatctaa atcaaacgtt tagttcttga ccaactaata
     7921 aagaataact ggacatagac atgccatttt tcgccattag atcatcaaca gacatgccaa
     7981 atttttgtgc aatatcccat agcgtatcac ctgacacgac tgtatatgtc tgaccattat
     8041 ttgtatttac atctgttgtt gaagatgatg acgttgaagt cgtcgtagta gtcgttgttg
     8101 ttgtacttgt ggttgttgta gccgaacctg ttgatggcgt atcaaaacgg gttaaattat
     8161 aagtattaat caatgagttc aatttggccc cataatttgg atcggtagca taacgacctg
     8221 tcaagtacgc tgttgcatca taaaaacttg ttgtattgct tttccacacg ccagcataat
     8281 gataatcgcc cgtggaaaaa ctggttgaac gaagaacatt cgcatggtct tggaaggatt
     8341 cccaataaga agaatatttt cggaatggct cattcatcgt tacccattgt ccgtttacat
     8401 attctttggt tgccatataa acgacctgtc cgttatagct tcctttcaca ccgaatagat
     8461 tataatttgg cgcactagct aatccggaag tgccatatgc actttccaac aacgcttgag
     8521 cgatcatgac tgaagcatac aagtcattac ttgcagcaac ttctgacgca gaccagccga
     8581 tttgatcgat aaacgcttga ggatcagcga ctgtttgaga tccttgttca tctgcatgaa
     8641 catgttgcgg taccgctgta accatcatcg gcccaactaa aagactcgtt ccaattaaag
     8701 gagcaatcgc ttttttattg atcaccattt ttcttttagg tctatggtgc cgggcactgc
     8761 gggtcttcag ctcttgcatt caaattcctc ctcaaaataa aaaaacttaa taaaaagttc
     8821 tcttcttaaa acaaacttaa ttaattaaat tatactttta att
//
LOCUS       DAMYXM010000092         8756 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.095, whole genome shotgun sequence.
ACCESSION   DAMYXM010000092 DAMYXM010000000
VERSION     DAMYXM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.095"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(74..694)
                     /locus_tag="PNS21_002501"
     CDS             complement(74..694)
                     /locus_tag="PNS21_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDI5160159.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(770..1207)
                     /locus_tag="PNS21_002502"
     CDS             complement(770..1207)
                     /locus_tag="PNS21_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDI5160160.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            1370..2200
                     /locus_tag="PNS21_002503"
     CDS             1370..2200
                     /locus_tag="PNS21_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDI5160161.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            2184..3551
                     /locus_tag="PNS21_002504"
     CDS             2184..3551
                     /locus_tag="PNS21_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDI5160162.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            3570..4379
                     /gene="yidA"
                     /locus_tag="PNS21_002505"
     CDS             3570..4379
                     /gene="yidA"
                     /locus_tag="PNS21_002505"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDI5160163.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(4422..4865)
                     /locus_tag="PNS21_002506"
     CDS             complement(4422..4865)
                     /locus_tag="PNS21_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDI5160164.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            4999..5370
                     /locus_tag="PNS21_002507"
     CDS             4999..5370
                     /locus_tag="PNS21_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI5160165.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(5539..6321)
                     /locus_tag="PNS21_002508"
     CDS             complement(5539..6321)
                     /locus_tag="PNS21_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDI5160166.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(6318..7289)
                     /locus_tag="PNS21_002509"
     CDS             complement(6318..7289)
                     /locus_tag="PNS21_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDI5160167.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            7381..>8756
                     /locus_tag="PNS21_002510"
     CDS             7381..>8756
                     /locus_tag="PNS21_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDI5160168.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRSNQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEK"
BASE COUNT         2705 a         1789 c         1495 g         2767 t
ORIGIN      
        1 aaaaatcgtg tcacacctcc ttattctgat aggctgatcg tctctgtcac aacctctcgt
       61 aagaaaataa tgtttattct ttttcagagt cagaaaattc ttcctcttca taatcttcat
      121 catcatcgtc ttcgtctatg atacttaaat cttcttcgat atttcctggc aagtcttctt
      181 catcatctgt cgggtcatct gctccgattt cttgcaagtc agaattataa gcagcgattt
      241 cttcatcttc atcatcatcg tcatcaaaca gatcatcgtc atcttcttct gtcagatctg
      301 tatcttctgg gtcatcgtca ttgtaatcga tcgcgtcttc atcatcattg ataaaggcgt
      361 tgactttctt gcgtttacgg cgacgtggag tatcttcatc ctcttcgtcc attccatggt
      421 ttacttcttc atcgatcgaa tcgatagcat accatgaacg caagccccaa cgattatccc
      481 ctaatgagat aaagctgccg tcaatattca agtcagtata aaattgtgcg agtgaatcac
      541 gaatttcact gtctgatttc ccaagatagt tttggatctg gttagctaaa tcagaaaaat
      601 ccatgacatc gccacgttgt tctaagatcg cgtgtgctac ttcgatcatt gataattcgc
      661 ttttgtttgc accttcaaat acactaattt ccaatcagga cacgtccttt cacagtctac
      721 tccttatcat acaaaatcag acacaaaaaa tcaaactttt cttttatatt tacgctgtaa
      781 aatccaaggt caaataatat tcgccgattt tttccggtcc catgaacaga tcataatcga
      841 ttcgaacctt ccctgctgtg ggacggtctt tcaaactaac atgcagttct ttcgtaaaag
      901 tagcaatctg gatcataccg tatggtgttc gataggatgt ttctaaccgt tctttatagc
      961 caaatttcaa acgcatccgc atttctcctg cacggatcag ctgtacatga ccatctggaa
     1021 ctaatttgat cgttacagga atctcatcgc ccgtctcctc ctgaatctct ttataacgta
     1081 tgtacagcgt atcgccaatt gtcaccattt gccctttcaa atcaaaataa aaatcttgca
     1141 cttccccttg ctgttttact tttgtacgca atttgattga aatcggtgtt ccttgtgata
     1201 attccattat tcattctcct ttacttctca tagctacatt gttcctagtt tcttttgttt
     1261 tgtcaaccaa cttgtccatt tttacagaaa accttttttc caaaacaact ctttgatttt
     1321 agagtataat attgtagaaa atatgaattt atacaaagga gccggttgaa tgaaccctgc
     1381 gattcgacta gatcagcaca tttatcaaaa aaacgacttt tccccttttg cattaacaga
     1441 tatattaacg gaatattcta aagaacacca gcaaatcttt ttacatttct ggcaatatga
     1501 ctgcacagtc atcttaggta tgaaggatgt tcgaactcct tttctaagtg acgggatcca
     1561 aacactggaa gatgcaggtt acgctccagt gatcagaaat tcaggtggat tagggatcgt
     1621 atcagatgaa ggaattttga atatttcttt gattttcccc caaagtgaca caaaaaaaat
     1681 aaccatagat gacgcttacg agcaaatgct cctattgact aaatacgctt ttccaaaggc
     1741 accgatacaa gcattcgaaa tcacccattc ttattgtcct ggaacttatg atttaagcat
     1801 ccatggcaaa aaatttgccg gtatcgctca gcgtcggatc aaaaatggga tttctgttat
     1861 gatgtactta agtgtcaacg gtaatcagga aaaacgtggg caagtcatga agcgatttta
     1921 tcaatctgct ttaaaagaag actttggtac aaatggctat cctgcagtgg atcctgctac
     1981 aatggcaaca cttgaagaac tgcttgacca accccttttt gtaaaagaag tcaaagaagc
     2041 atttgcaaaa gcttttgaaa agctgtattc tgcaagtgat ttacaggatt ctaccgactg
     2101 gctaaaaaaa gaaatccagc tggagcaatg gaacaaccaa atccagagaa tggaacaacg
     2161 taataacata aaggagctac attatgacaa ccccttataa atatcaaatg ctgcctatgg
     2221 aaaaagtttt tcgtgatcca gtccataact atatccatgt ccagcatcaa gtaatcttag
     2281 atttgatcaa ttcgaaagaa gtccaacgtc tacgacgcat taaacaatta ggcacttctt
     2341 ctttcacttt tcacggagca gaacatagcc gttttactca ttcattaggc gtttatgaga
     2401 tttcaagacg aatctgcgat atcttttctc gaaatttttc aaaagaaaaa ataggcaacg
     2461 gaggctggga tgatcaggaa cggctggtga ctttatgtgc ggcactgttg catgatgtcg
     2521 gtcatggtcc ttactctcat acatttgaac atatcttcca tacgaatcat gaagccatca
     2581 ctgtagagat catcacttct ccagaaacag aagtgtatca gattcttaac cgagtagaag
     2641 aaggttttcc tgaaaaggta gctagtgtga tccaaaaaac ttaccccaac ccacaagttg
     2701 tccaaatgat ttctagtcag atcgatgcag atcgaatgga ctatttgctt cgggatgctt
     2761 atttcactgg aacagaatat ggaacatttg atcttacccg aatattacgt gtgattcgtc
     2821 cttatgaagg cgggatcgct ttttccatga gtggcatgca tgcagtggaa gattatatcg
     2881 ttagccgata tcaaatgtat gtgcaagtgt acttccatcc tgtttcccgt ggtatggaag
     2941 taattttgga gcatctgctt catcgcgcac acgagttgta tcaagaagat gccagcagtt
     3001 ttgaactcca ttctcaatta cttctgccat ttcttgacgg aagctttact ttagaagaat
     3061 acctcaaact tgatgatggg gtcttgggca cttactttac tcaatggtct gaatctgctg
     3121 atcctatttt aaatgattta gctaaacgtt ttttgaatcg taaaccactg aaatctgcta
     3181 ctttttctgg caaccgggat tcaaaattag tccaagaact cactttgtta gtagaaaagg
     3241 tcggatataa tcctgtttat tacactgctg tgaattctag ctacgatcta ccttatgatt
     3301 tttatcgccc agaacaaggc agacatcgca cacaaatcga gattttgcga aatgacggaa
     3361 ctttgataga attatcacag gttagtcagc ttgttgctgc attagcggga caagaacaag
     3421 gagatgagcg atttttcttt ccaaaagaaa tggttgatcc gtccttgcgt gatcattatg
     3481 atttatttga cgaaacttat caagaatttg ccagccatat ccgtaatggc gcattgattg
     3541 aaataaatta aaatatattg gaggaaatta tgtctatcaa actagtcgct atcgacatcg
     3601 atggtacttt attaaatgag aaaagagaag taacaaaaga agtaaaagaa gcgattgccg
     3661 ctgctgttgc aaaaggtgtt tcgatcgttt tatgcactgg ccgtccctta ccaggtgtcc
     3721 aggaacagtt aaacgagttg aacctttttc aagataatga ttacgttatt acttataacg
     3781 gggcactcgt acaacaaacg aaatcaggta aaatcatcgc ccgtcacgga ctgacacatg
     3841 aagatttcct agagatcgaa gtaatggcac gccgtgtcgg cagtcatctc cattcgatcg
     3901 atgacaaagc gatctacacg cctaatcgag atatcagcgc ttatacgatc cacgaagcaa
     3961 gtttagtcaa gatgccgcta aaatatcgaa cacctgaaga aatgacacca gacatgaaca
     4021 tcgtcaaaat gatgatgatc gacgaaccag aaattttaga tgcagcaatt gcgcgtttac
     4081 cgcaaacttt ccgtgataaa tacacaacgg tcaaaagtgc gccctattac ttcgaagtac
     4141 taaataaaga agcaagtaaa ggtgcggcag ttgcgaatct cgcacagcac cttggtattg
     4201 atcaagatga aatcatggct atcggagata acgagaacga cttatccatg attgaatatg
     4261 ctggtctagg agtagcaatg ggaaatgccg tttctcttgt caaagaagca gcaaatgtca
     4321 tcacttcatc aaacgacgag cacggtgtag ctgaagcaat caaaaaatat gtactgtaat
     4381 aaataaaaaa actgagagaa agctgatcta ctcgctttct ttcagttttt atttttttcg
     4441 atccatctct cgactgccca tcggtggcta ccacgtttca atttcacaag cgcttcttga
     4501 gggctcaccc accaaatttt attggtcttt tcagttggtt ctcctatacg cttccaagaa
     4561 tcagcaacaa agaaatatcc tggattataa taataagttg cacgataatt cgagtaaaaa
     4621 tactcatcag cttctcccag ataagaagaa atttctgcca caattcccat ttcttctatc
     4681 agctcacgga caatcgcttc ttctttcgtc tcttctcctt caatttcccc gccaggaaga
     4741 aaaaaagcac catttggagc ctcgattact gcaatctgct gatctctttc tatgacaata
     4801 tatgctgcat aacgggtttg ataatcagca aattgctttt tttcaccaaa aattggttta
     4861 tccatgaata gtttccacct taacagtttt attttctatc cattttacat gagaaatttc
     4921 tcatgaacaa gcacaaatca actatttcct tacttctttt tgttatgatt gagtaaaagg
     4981 aaaaggagag atgaatctat gaaaatggct cacacatgtg ttcgagtgaa agatcttgaa
     5041 gcatcaatcg aattttatca aaaggcattt ggttttgaag aaagccgccg ccgtgatttt
     5101 ccagaaaaca aatttacact tgtttatttg acacttcctg gggatgacta tgaattagag
     5161 ttgacttaca actatgatca tggtgcgtat gatctaggag acggctatgg tcatatcgct
     5221 atagcagctg atgatctaga aaaattacac gagcaacata aagctgctgg tcttgagatt
     5281 actgacttaa aaggattacc tggcactgcg ccttcttact actttgtcat tgaccctgat
     5341 ggttataaaa tcgaagtcat ccgaggatag ttagtatggg gcattttact cttgattgaa
     5401 taaaaaacaa acaccaataa ttaaaatgag acgcaaactt ttcattaaat tgaaaaagtt
     5461 tgcgtctttt tttaacaacc attgtagcta taaggttgcc actctccacc aatttatttt
     5521 taattcattt gaaagaaatc aaataaacga ggtaccttta gaaaacaaaa ctgcttttcc
     5581 tcctattatc acacgatctt cttttaattc acattctaga tccccgcccc ttgatgacat
     5641 ttgataagct gacaattttg ttttattcat tctttctgcc caataaggaa taagattaga
     5701 atgtgctgat ccacatactg gatcttcatt gattgttaat tttggaaaga aggttctaga
     5761 aacaaagtct ttttgcacac tttctgctgt cacgatgact cctattccta attcaaaatt
     5821 tttgatagct gtaaaatctg gctgcaattt agccaccgtt ttttcatctt ctaatacgaa
     5881 aaataaatca cgtgctaagt aggcttcttt aatcttagcc ccaatcgctt cttcatattc
     5941 agacaatatc ggcacttttt tcggcataat actaggaaaa tccatgtaat aataatcttt
     6001 aattcttgtc acaaatagat taccgctttg agtagaaaat ttaatcgttt catcaggtat
     6061 tttgtaatag ttaaataaaa caaatgctgt tgctaatgtc gcgtgaccac aaagatcgat
     6121 ttctcgatct ggcgtaaacc atctaagctc aaattcttgg tttttcttca ctgtaaatgc
     6181 tgtttcagat aaattgtttt caatggcaat tttttgcatc gtaccttctg gcagccattc
     6241 ttctagcaca taaaccgctg ctggatttcc tttgaacacc tcatctgcaa atgcatcaac
     6301 tacgtaataa tctaagttca ttttaaagcc cctttctcta atttgttata aatatattct
     6361 gcacaacaaa gatcttcaat agctagacca actgcgtcga aaatcgtcgg cctcgattct
     6421 cctttataaa atgttttttt ctcagaaact aattcgccta atgtaccaac aatacaagat
     6481 tcctctaaat ctccttctgc catggctata agcaggtccc cgctttctgt taaggcagct
     6541 gagtaatcat ctacatagat ttgagaagtt tccatcaacg atgtacttat ctctcttgta
     6601 tcaggagtaa atgtgcccac agcattgata tgtgcgtctt cccttaccca ctctttttga
     6661 agaaatgcct ctttactaga ggtcaacgta caaatgattt ccgctggacg tactgcatct
     6721 tctactgata ttgcatttat aaacgttatt ttaggatact gttttttagc tttagcgata
     6781 aaagtatttc gtctttcttc taaaatatcg taaacaaaca ctgtagtgat gggacgaatg
     6841 gtagaaatcg cagtcaaatg cgacaatgct tgttgaccag ctccaattaa agcaagtgtt
     6901 tctgcatgcg gatttgctaa gtaatctgtt gccagcgcag atacagcagc tgttcgaatc
     6961 caggtgactg catttgcgtt tatcaatgct ttgggtagtc cgttttgagc atcaaacaag
     7021 aaaatctctc ctaaatgaga aggtaaatga tactgatgat tatccggaaa agcggtaatg
     7081 acttttgtgc caaaatatct gttctccccc aaataagcag gcatcatcgc aaacatattt
     7141 ttgccttttc cgtcaggtaa aatttccacc aatcgaggat tttggctgat tttaccactt
     7201 tcataatcaa aaaaacattt tttcattact tgaatcgctt cttcaaaact aaaagaagct
     7261 actacttcct caaaattaat aattttcata gacaattccc tcctcacaaa agattaactt
     7321 ctaaaatcaa tacaatcaat ggtaaaattg tatggtatac aattttaagg agtggagaaa
     7381 atgcctatta attcttatga caattacccg ttaacttggc gaccagacaa acaaaaattg
     7441 aagcgtcctt attatcgatc gttgattcaa caattagaac aagatatttt aaagaataaa
     7501 ttacagaaaa acactcagtt accctcccaa agagagctag ctgattattt agatttaaat
     7561 tttactaccg ttggcaaggc ttataaatat ggaattgaga aaggtttgct gtatacgaat
     7621 attggaagtg gcacatttat ttctcccaat gtctttgagt ccatcaccat ctctaccaat
     7681 gaagtaaccg aacatatcat tgattttggt ctggttagtt cttttgaaga atgtaataaa
     7741 ttcgttgtcc cgtttattca atcaactagt aataacccaa atcttgttgg tttgcttaat
     7801 taccaagatc ccaaaggcac aaattttcaa ttgactactg cagcaaattg gttaaaaact
     7861 caaggagtca ttccgtcctt agaatctcta tctattgttt ccggtgttca aaatggattg
     7921 gctatcacac tttctgcact tttctctcca ggtgatagaa tagcaacaga ccgatataca
     7981 tacgctaatt tcattgaatt ggctcagtta tatcatttag agatcgttcc aattgatttt
     8041 gacgagcaag gaatgcgacc tgatatattg attcaggaat gtagaaaaaa gaagattcat
     8101 ggtgtctttc ttatgccttc ttgtaataat cctatagggt ttcaaatctc tcacaaaaga
     8161 cgagagcaat tagccgaagt gattgaaaaa gaaggtctat gggttattga agatgatatc
     8221 cattcatttt taacaactta ctatcaacat acgatcattc ctcctttcca aaatttatta
     8281 cctaaccaaa caatctactt agctggaatg acaaaattta tttgttctgg attgcgagtt
     8341 gcttatcttg tagttccagt acatgtccaa agcaagatag aacaagcaat ttttaatata
     8401 aatgtgaaaa cttctggatt agatgtggaa gtcatcactc aaatcctcca ttcaaatgaa
     8461 gcaaatagga tcttagaaac taaattaagt cttacaaaaa gatccaatca gctttttgat
     8521 tctcttttca aagtagaaaa accaacgaat cgtgtccctt actatcgaat tcttccagtt
     8581 gatcctgaga ttgaccctaa gataattgag aatgactttc ttgctaacgg tatccgactt
     8641 tatcattcca gacgctttac tacacgtgcg caaccagatg cattcgttcg aatttcccta
     8701 tcctctaata accttgaaat tttagcttct ggactaagta tcgtaaaaga aaaaat
//
LOCUS       DAMYXM010000093         8232 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.096, whole genome shotgun sequence.
ACCESSION   DAMYXM010000093 DAMYXM010000000
VERSION     DAMYXM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.096"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            331..1254
                     /gene="truB"
                     /locus_tag="PNS21_002511"
     CDS             331..1254
                     /gene="truB"
                     /locus_tag="PNS21_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDI5160169.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1258..2199
                     /gene="ribF"
                     /locus_tag="PNS21_002512"
     CDS             1258..2199
                     /gene="ribF"
                     /locus_tag="PNS21_002512"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDI5160170.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2594..3379
                     /locus_tag="PNS21_002513"
     CDS             2594..3379
                     /locus_tag="PNS21_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI5160171.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3392..4243
                     /locus_tag="PNS21_002514"
     CDS             3392..4243
                     /locus_tag="PNS21_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HDI5160172.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4367..4819)
                     /locus_tag="PNS21_002515"
     CDS             complement(4367..4819)
                     /locus_tag="PNS21_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5160173.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5284..5652)
                     /locus_tag="PNS21_002516"
     CDS             complement(5284..5652)
                     /locus_tag="PNS21_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HDI5160174.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5802..6284
                     /locus_tag="PNS21_002517"
     CDS             5802..6284
                     /locus_tag="PNS21_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HDI5160175.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6321..7331
                     /locus_tag="PNS21_002518"
     CDS             6321..7331
                     /locus_tag="PNS21_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDI5160176.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7446..8159)
                     /locus_tag="PNS21_002519"
     CDS             complement(7446..8159)
                     /locus_tag="PNS21_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160177.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2785 a         1337 c         1668 g         2442 t
ORIGIN      
        1 tgattaagaa actgaaaaaa taaataaaaa aaaacggaaa aaagactgga agaatggaca
       61 gtcttttttt tcgtttttgg gaagtgaaaa ccaaactatt tacctgtaag ccacccataa
      121 atccaatcaa atggtgcaaa gaaaaaatct tccattctat tccctccttt aaaaaaaaca
      181 taacatgatt tctatttacg tgagcatgaa ttttgttacg tttttattgc cgaaaaataa
      241 aaaccgttca ttatgatgtc aaattcgtca gtaattgatg caatgtgtta taataattca
      301 gttaaatcaa aaatgaaatg gagaaaagac atggacgggt tattaacttt atggaaagaa
      361 cgcggaatga caagtcatga ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa
      421 atcggacatg ggggtacgct tgacccagac gtagacggtg tgttgcccat ttgtattgga
      481 aaagccacaa aagttatcga atatttgaca gattctggga aaatctatgt aggcgagata
      541 actctaggtt tcagtacaac gacggaagat cgatccggag aacgggtaga aacaaaacca
      601 gtagatatgt ctttatctga agaaaaaata gatgaagtga tggcttcatt tattggtgag
      661 atcacccaga taccaccaat gtactcagcg gtaaaagtca acggtaagcg gttatatgaa
      721 tatgcccgca ataatgaaac agtggagcgt cctgttagaa aggctcagat ttaccgtttt
      781 atccggacaa gtgaattgac aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa
      841 gtagaatgcg ggaaaggaac ctatgtccgc acattagctg tagatacagg caagaaactt
      901 ggttaccctg cgcacatgtc tgatctgacc cgaacagcaa gtgccggtat gtcccagaaa
      961 gatgctctga cattagaaga agtagctcaa gctgtgaaaa atgggacaat cgatgattgt
     1021 ttgctgccaa tagaaaaagg agtgaaacga ttcaaacgag tagatattac tgatgaagta
     1081 tggcagaaag taaaaaacgg tatgagactt aattatactg tatttgcgct cccagaaatg
     1141 ccgtcagaag aaattgcttt gttttataaa ggcaaagttg tcagtatcta tcagccaaac
     1201 ccgaaagaaa aaaacaaatt aaaacctagt aaagtattga gaaatgagga ataacgaatg
     1261 aatatcatca agatcagaca tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt
     1321 ttagtactag gattttttga cggcgtgcat attggacacc agaaagtcat aaagacaggt
     1381 atggaaattg cacaaaaaga aggattgaaa ttagcattga tgactttcaa tcagcatcca
     1441 tcgattgtct ttaaaaagat cgatcctagc agcgtgaaat atttgactag ccttaagcaa
     1501 aaagaagaaa aaatggccgc tttgggtata gattatttat atgaagttga ttttacttct
     1561 tcttttgctc atttagcacc gcaggaattt gttgatcaat acatcgtagg gttacatgca
     1621 aaatatgctg tttccggttt tgattatacg tatggaccaa aagacattgc agatgtcgct
     1681 cattttcctg aatatgccaa aggaagattc aaaatcgtca cagtttctaa agaagaagaa
     1741 caaggtgaaa aaatcagctc tacccgtatt cgaggattac tagacagcgg acaagtaaaa
     1801 gaagcagcaa aactattagg ttgcacatat gaggtagaag gggtagttgt ccatggcgat
     1861 gcacgaggca gactactagg attccctaca gcaaatgtca aagtaaagag cacagttcat
     1921 ttgcctaaag aaggtgttta tgtatcggaa ataaaaattg gggaaatttg gtatccagcc
     1981 atgggttcaa ttggacataa tgatacattc ggtgaaggac gggagctgac tgtagaattg
     2041 tatattttgg actttaatca agatatctat ggtgaacatg tcgcagtacg ctggaatcaa
     2101 tttttaagag atcaagtgaa attttctagt gtagaggaat tgattgaaca actcaaagag
     2161 gatgaacaag caacagctga atatttttct cagcaataaa acattatgca taaaatctct
     2221 tgtttatgta taactagcaa tttcaataaa atgggtgata aataagccca ggaaaggggc
     2281 tgtgacaaaa gtcttgtcac agcctttttt tccaaataaa tggtttttca gaagtagctt
     2341 cttcggaaat aagccgaaat ttcacaaaaa tttgagaagt aatttttgta aattacttct
     2401 tatttcttga agctggccac ttctgtcaca acctctttct ttatagaaat aaaaggacac
     2461 tagcaattca tgaaaacgat tgctaaagcg tatcatttgc tttagactat atatcttggt
     2521 gtacactcac tttaagacgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa
     2581 aggagtgggt actatgtata aggaattaga aaacaaagtc gcagtagtaa ctggtggctc
     2641 aaaggggatt ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt
     2701 caactataac tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg
     2761 cggaaaagca gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact
     2821 tgatacggct ttagaaaatt ttgggacact ggacgtttgg attaataatg ctgggatgga
     2881 aaatcagcat ccaacccatg aacttagcct tgaagattgg gaaagagtat taaaagtcaa
     2941 tttgactggc gtttttatcg gaacgaaagc ggctttaaat tactttacag agaatgataa
     3001 aaaaggaaat atcatcaata tgtcttctgt tcatgaacaa atcccatggc caacgtttgc
     3061 tcattacgct gcttcaaaag gcggagtaaa actattcacc gaaacggtag caatggagta
     3121 cgcaccaaag ggaatcagaa tcaatagtat aggacctggt gctatccgta cacctattaa
     3181 tgcggcaaaa tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg
     3241 acgcgtaggt gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc
     3301 cagttatgtt acaggtatta cactatttgc agatggtggg atgactttat atccttcttt
     3361 ccaaggtgga aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc
     3421 agttgggtgg gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga
     3481 gatattagga actggtattg gagccgtgct cgttggctta cttgttcaat tattcgcttc
     3541 tccagctggt atttcagtga ctacattttt gattagttta ctttctggtg cattctgggt
     3601 catcggacaa acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc
     3661 aatttcgact gcattacagt tagtcggaac ctcattgatt ggtgtcttcg cttttggaga
     3721 atggtctgga agtatgggaa aaatcattgg tgcagcagca atcattcttc ttgtatttgg
     3781 ttctgcttta acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat
     3841 cagtctatta gcatcaacaa gtattggtta ttgggtttac agtgcattgc ccaaactagt
     3901 tgatgcaagc ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt
     3961 gatttatgtg attttccgaa aaccaaccgc attttctgaa gacaagagtt ggaaagcaac
     4021 aatagttgga gccatcttca gtgtgtcagc attggcttat attttttcag caaatgaaaa
     4081 tggggttgct actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg
     4141 tatgtacatc ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt
     4201 attgctgatc gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa
     4261 tctcatttat taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc
     4321 gctagaattt ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaagtg
     4381 aaatatctgt ctgcatcaat aatttttcat tgatccattg actaaaattt acgacatgat
     4441 tgaagttacc accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa
     4501 gacggtcttt tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggattt
     4561 gattgattgc tgtaaaatca cgatactgaa ggttgaaacg catcgtgatt ggtgcattta
     4621 gcatgaatcg ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa
     4681 aaagattacg ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt
     4741 catgaagata atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta
     4801 ctgagtaaaa atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga
     4861 tatttgctta atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca
     4921 agtcagaaaa ttctaacttt tctttctcta aagaaattcg ttctctccta aagtgatatt
     4981 atatttaaat atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg
     5041 tattttttcc ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt
     5101 ggaaaattca aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa
     5161 aatagcagat actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga
     5221 acaaaacctg tataagctga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa
     5281 gctttatcgc caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg
     5341 ttcaatcaat tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata
     5401 tacgtagcct gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc
     5461 gatagaaaaa tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa
     5521 cataggttgg ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc
     5581 aaaaaggtct ttgatctttt gttgatgttc tttttctttg atctgattga tgatcggctg
     5641 aaatttttcc attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca
     5701 atagttatgt ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg
     5761 ataagatgtc tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga
     5821 aattcttaca cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg
     5881 aagatgcttt ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat
     5941 tcttaaaaga taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg
     6001 atttcaaagc attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca
     6061 tggaagaaca aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga
     6121 ttccgattat tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta
     6181 gaccagatca aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg
     6241 agatgccgat atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc
     6301 atacttggag gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg
     6361 gggttactgc accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt
     6421 taaagagaat ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag
     6481 ataaggaaaa atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag
     6541 atgaaaatat ccaaatgatc tttccgcctt ggggaggaga attattgatt gagattctcg
     6601 attttgtcga ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta
     6661 cgttgctact tgctgtcaca ttaaaaacag ggatagccac tgcacatgga accaacctta
     6721 ttgatttaag aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa
     6781 caagagaagg agaatcgatt ctacagcaac cttctgaaaa gtatcaaaaa gagtggcccc
     6841 atgagcattc atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca
     6901 gcggagatgc agtaaggatg aagggacgat tattaggtgg ctgtattgat acgatcagtc
     6961 atttaatcgg taccccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa
     7021 agccgatctt gtggtttcta gaaaattgtg agctatcgtc agttgattta agaagatcac
     7081 ttgttcagat gaaactggca ggatggtttg atggcagctc ggggatcatg ttcggtagaa
     7141 gtgcagcaga ggatttgtta gattataaaa tgaaggatgt atatgaagat ctgtcaaagg
     7201 aattaaaact accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat
     7261 tgatcaatgg tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa
     7321 cgtttattta aaaaattttt tcctgccttg gagtttatca aagaccaaca aatctaaata
     7381 gaaaagaaaa tgaaagaagc tgacagtatt ttgccagctt ctttttgtat gatctattag
     7441 aaattttaat tatccataac tatcacattg atcagctgtt tcacatcatt tggattacct
     7501 gtaggaatag ctactacaga tgcattatca ataatttctt gagttgtttc tctaatgaac
     7561 gctggataag atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg ataactagtg
     7621 attgtcccgt catggttact tgaatacact aaaactccct ggtaactgta tttcccagtg
     7681 agcataacca catctttcgg atagactgca ctccctgcat cataaggggc gataggtgtt
     7741 cctgcaggat aatgaataac atgtagttcg aaatcacccg atgtatctct aaaagtatta
     7801 cctctaattg ctagccaaac acgtgcgtac tcaatttgta gatcagaata tcctgctaac
     7861 ggatctattg actgagaaga ctgtgaggaa gttgtgtcta ttgttgaaga agaaatattt
     7921 gtctcaactg tactactaga tgtagaagaa gtattgggct ctgagacttt actgcttgtt
     7981 gaagatgtat tcgtttcagt agaagctgat ttctgagtac ttgatttttc agctgaactg
     8041 gtttgtttga ttgaactttt gtctgtggtc gcagatggat cactattttc tttactagga
     8101 gacgtacatc ctacaaggaa taaagagacc aacaataaaa taaaacctaa ctttttcata
     8161 taaaatacct cattattttt ttaataccag tatagcataa tcctgaattt ttgaatccgt
     8221 aaaaccttgt ct
//
LOCUS       DAMYXM010000094         7969 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.098, whole genome shotgun sequence.
ACCESSION   DAMYXM010000094 DAMYXM010000000
VERSION     DAMYXM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.098"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            107..3385
                     /locus_tag="PNS21_002520"
     CDS             107..3385
                     /locus_tag="PNS21_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HDI5160178.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3926..5833)
                     /locus_tag="PNS21_002521"
     CDS             complement(3926..5833)
                     /locus_tag="PNS21_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HDI5160179.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5963..6838
                     /locus_tag="PNS21_002522"
     CDS             5963..6838
                     /locus_tag="PNS21_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HDI5160180.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6918..7892
                     /locus_tag="PNS21_002523"
     CDS             6918..7892
                     /locus_tag="PNS21_002523"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDI5160181.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
BASE COUNT         2557 a         1477 c         1681 g         2254 t
ORIGIN      
        1 tttttataaa atacacttat tataattttg ttttttattg acagtaaaaa taaattaggt
       61 aatactttaa gtaaacgctt tctaatttgt tacgggagga atacacttgt taaaaaaacg
      121 tataaaatac agtgcgctaa tgatgaatct ccttatgctc agtaccccgg ttttaatttc
      181 tgtcgttgaa gttgctgcta acgaacaaca gtctgtgaac gaagaaaatg cacacgtgaa
      241 cattgaaggg atgtcaacaa tggaggaaat accaagaact agtgaagata tggatacgga
      301 agaaggaaat gatcctatga tgaaagaatc agaaaatgaa aagacaaaaa agcaatctga
      361 agaagcaaat gaagagtgga aaggggttgt tttcggagaa agcacaagtg ctagtaggta
      421 tgccattgaa ccattagaag acggcgtgcg gctctcttcc acgaataacg gcggaaaatt
      481 ccagtcaagc ggatcagatg gaatgaccta ttattatcaa gcaatcccca aagacatcaa
      541 ctttaggatg cgggcgacga tcgaaataga atcttggacc tatacgaatg cccaagaagg
      601 atttgcttta atggtaagag acgcagtacc aaaaaatcat ttgtttgggc aggcatttta
      661 ttcaaattca tttgccatac taggcagtcg gatcgaatat gtctgggact cggatagaca
      721 agaagtggtc aacacgagcg gcagcaaata tacgatgcga cttggattgg ggacacgggc
      781 aatcacagga atatcatctg aagatccgca agctgcgcca gctcctggga gtgtctcagt
      841 ggaaacaaca cctttagaga cttcagcagg gttcttggaa aaagaaacag gttcttataa
      901 tatattcgga aacggtcatg ggaatctgtt tccggccacg aactcgatta ctaaactgga
      961 cgtagagatg aaaaaaacga atactggttt agaggcatat tattacgatc ctgaaacagg
     1021 agaagaaatg gcttctgaca tcatgtatga ctgggaaaaa ttatacgctt cagatgaatc
     1081 ggttatttat gcaggatttg ctgcagcaag gaatatgacg atcaaggtag aaggaatcga
     1141 agttgcttac tcagatcctg cgacagatcc tcctgggcaa gaaagaccta ctcgcttgat
     1201 tgatccgatt tacactgtga cctctagtaa tcattcagga aaccaagatc atactatgtc
     1261 ctttagagca aatgctgacg gattattgac aattaaaaat aaagcaacag gagaggtgct
     1321 taagaacgca aaaaacttgg ctatcactgc gaatcgggaa tttgattatc agacaaaact
     1381 gatggaagga acgaacgaat ttactttcag ttttacgcca gataaaaact atccgccaga
     1441 agaatacgta gagatgacta gttatgaaac aaaagagatt cttcatacag tggactaccg
     1501 taaacctaaa tattcgacag tttatgtaac accagaaggg tcagatgcag gaaacggaag
     1561 cagacaaaat ccattatctc tgacacaagc attgagatac gcatatgctg ggcagacaat
     1621 cgtcatggcg cctggaactt attccttcga aagaggattg acgattccaa aaggcgttaa
     1681 cgggacaaga gaaaacccga ttcagttgat tgcagagaaa aatccagaaa acaagcgtcc
     1741 tgtacttgat tttaaaggaa ctggaaatgg gatgacactg ttcagtcact actggggttt
     1801 acgagggttt gacattacaa atagtgcagc tatgcaaaaa ggattacaga tttctggaaa
     1861 tcataatttg atcgaagaga ttcatgctta tcggaatgga aatacaggaa tccaaatcag
     1921 cggtagctca aatgatcctt tcgaagcatg gcccgcgcat aacttagtca agaactgtac
     1981 atcttttgaa aatgccgatc caggatttga agatgccgat ggatttgcgg ccaaactgac
     2041 aataggagaa ggaaatgtat ttgacggttg tatcgcgtat cataatgcag atgacggttg
     2101 ggaccttttt gctaaaaatg aaacaggttc gatcggaaaa gtaatcatca aaaactcagt
     2161 tgcttatcga aacggctggg taccaggaat tgagggagaa ggtaacggta atggattcaa
     2221 aatgggcggt tcttctttaa cgggccctca tgaattgatc aatagtttgt ctttcgaaaa
     2281 tcttgcgaaa ggaatcgatt ccaatagcgg aacagatatt atcgtcaata gcagtacaag
     2341 ttttaataac ggttcatata atgtggctct ttatacctct tcagcaggaa acacagacta
     2401 ccatgcttct ggtatcttat ctttccgaac agagaatctt gagatgagag agcagatccg
     2461 cccattgaat caaaacgaag atcaaatcta ccactcgttg aactactatt ggaatgaacg
     2521 agagaaacaa tcagaaaata ctcttggaca taccatatca aaagattgga tcgagtctct
     2581 aaatacatgt tcgaaagaag gtcagcagcc tgttacgcgc tatgaaaatg gaagtatcca
     2641 tgttcatgga ctgatgcaga ttaaaccggg gaatcgtcaa acagaaaagg aaagagtgga
     2701 tggtcttcca ttatctgtag gtgctatttt tgacggagaa acctcaccgt cttcggaata
     2761 tccagagtat gcaatcgtac aagaaccaaa tcagacaaaa ccaaatgaac aaaataatcc
     2821 gattattata caagaagcaa atggtgtggc ggagaatgca gaaggaaata tcattgaact
     2881 gaagcctttt acagtttttg tgtcaacttt cacaccaaat caaggaacgc ctgtctttga
     2941 agatatgcgt tatttcccag aaaagatcca aatcgttgaa acacagtcaa gtgctcaatt
     3001 ttctaatcat accgctattt tttcagcacc ttcaaaagga gaatatacgc tagaagtcac
     3061 ttatcgttcg gaaatatttg atggggaaaa atggtctgct catacagaat ctgttaccca
     3121 gcaaaaagcg atcactgtgc gagaaactgc cgatgtgccc ccgggaaatg acaaaccaga
     3181 aagagaaaat ccaggaaatg aatcaccaaa tggaaaacca aatggaaaac cttctcatcc
     3241 ggataggaca gcaaaagaaa gtgaaaaaaa tggcactcaa ggactcccaa aaacgggaga
     3301 atttaataac ccgttgctag cattagctgg aggaattttg ttaatcggtg tagttgttta
     3361 tgtcatgaaa cagcgaaaaa aataagtgga atttctcctt ttatgatgta aaacaattga
     3421 ctgaggaaag taaaaataga aggaaatgag accaatagat gtctcacttc cttctatctt
     3481 tttatagatg aggttctcct tgctgctttt gattggctgt tcttattact tgatgcagga
     3541 cagctttttc acaatctctc cactaaacgg tgttcaatca gctgaccctc ggtattagct
     3601 caacaaacgg caaaatatga ggaactattt ttccgttttt ttctctaata ctcaggtcag
     3661 gacgctgatt tcacaacctc tcgataaacg gtgttcaaaa gccagtttct cggtagtaag
     3721 tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa tttttcttac tacttgaaac
     3781 taagcagctt tttcacaacc tctaatacat ggtattccag aagtaacttc ttcgaaaaat
     3841 aagccgaaat gtcacaaaaa tttgagaagc gattttccca aattccttct tatttcttga
     3901 agctgaccac ttctggcaca acctcttacc agattcgcac tcttttttct ggcgctagat
     3961 acatagcgtc ttgttccgtg ataccaaatg tttcatagaa ttcgtcaagg ttttttggtt
     4021 gcacgtttgc tcggagttta gcaggggcat ggacatcgat ttgcagtaaa agttgttggt
     4081 attctttttt tgcttttgta cgccaaatcg ttgcccaatt gacaaagaat tcctcgatag
     4141 aaagatctga ttccgttttt gctgcttcca atgcacagct tagaccgcct gcatcagcaa
     4201 tattttccga aacggttaat tttccgttta cttttccgtc agcaaaaggc agaccatcga
     4261 attcttcgat cattgcttgt gctttgtttt gaaattgtgt aagatcttct tctgtccacc
     4321 agttgttcaa gttgccatat tcgtcaaata aagcgccgtt attatcaaat gcatgggaaa
     4381 tctcatgagc gataacagct ccgattccac cataattagc agagcttgat tgttctagac
     4441 tgtaaaatgg tgcttgaagg atcgctgccg gaaagacgat cacattccga aatggatgat
     4501 aataagcatt gaccgtgtca gcactcattt cccattctga acgatccact ggtttgttcc
     4561 atttgctgaa acgatctttt aatgtgagtt tgctgaaaac aagagtattt gaaagcaaag
     4621 taccaccctg atctgcaggt actgttttga actggtcgaa taatgctggg atcttatcgg
     4681 ggtagcctac ttggatacct aagttattta attttatgac ggctttttct tttgttgcat
     4741 ccccaagcca gtcattttct tctagacgtt ttttataaac ggcgatcatt tttttcacca
     4801 tttgttctac gtctttttta gcagcttcgc caaaatattt ttttccgtag taatcgccaa
     4861 ctacttgatc gaattgtcca gaggcaaggt aatacgctgc ttttttctga ggcattgctt
     4921 cgtctgttcc agacaatacg cgagaataga ttccgctgac ttggcggaat tcttctgata
     4981 gataaccact aagagaacgt actgttttaa tgatcatcca gcttttgaat aacggaaaat
     5041 gagcttctgt caggatgtca ttcaaatgat cgaagtacac tggatcagtt acgatcactt
     5101 tatctggagt tgtcccaatc aaacccgtga caagtttttt caagtccagt ttatctgtgt
     5161 atttaataaa tgtatcaaaa gattgcggat tatacatttt gctgtaatct gcgttttctt
     5221 ctgcactttt tacatgagga gcaatcaatt tatcaaattg cactgcttgt tccgtgattg
     5281 cttctgcctg acttttttct ttgccggcaa gctctaataa ctgaacagcc atatcaaaga
     5341 aaacgcctat tagttgtgca ccgttgggat ggtctgatgc ataatacgtc ttatctggaa
     5401 ggaataaaga aggcggataa gcaaagaagg cgttcgtttg cgcattcttc atatctgcat
     5461 ctacatccag attaaatggc aatggtaatg tatccaacag ccattctgga aattgcttat
     5521 tcaattcgtc gaaagactgc aatgattcaa ctttttcgat tataggttga agcggttgag
     5581 catgcaactc atttcttcgg tcataatcgt ttgccagttg ataatatgca aggaaatgtt
     5641 tcattttttc tgaagacact ttttctggat gggcaagcag atcgtctgtt tctcccatca
     5701 aaagttggtc gatcccgtca actaaatctt ggaaaccgcc tgtagctggt ttatctgcag
     5761 ggatttttgc tgttttttgc cattcttcat tgactgcttc atagaaatct tgtttcaatc
     5821 gttctttgtt cattcatcga tcgctccttt tttgtttcgt gtgcccatta tcttacaaaa
     5881 tgaaggaatt aacaaataga acataaaaaa ttgaaaattc gaacaatcaa actgatttgt
     5941 ttttttcaat ccttcactta taatgaaaaa aaggaggcga tcccaactga atcaagtagt
     6001 ggataaacct ttatatttca aagtagataa aaaaattaaa aaattagcca gcactcagca
     6061 gctgcagtca agaaaaagta aattgctttt tttagctctg gtttttgaag atcaaagtta
     6121 tgtgattatc gatcaatcag gacatccggt agaatattct ccagcaaaat atacttatca
     6181 agagggtctt tcttgtaaaa aatggaagct gatcaatgaa gagccaatcg agttatctag
     6241 atggatcaac agaaaagaag atattcccgt tttgatcgaa gaaaaaaggt caggaaagga
     6301 actcgcaaat tgctgggtgg gattgccaga agaacgtttt ttgaggtata agaaatgggc
     6361 taccccctca ggctatcttt gtggtactta cgcggcagca gttctccttg cctattatca
     6421 agactatcgt aaggggtgga tgcttccaaa tgagattcgc aagaaaaata cggctgattc
     6481 gttggttttg acgaaggcat tgcgtagcca gatccaacca ctaggacttc ctacgattcc
     6541 ttttcaggtt agtacaggga tttctagttt tttaaagaag aatggcaatc atgagcgtgc
     6601 acgagcaact cttctaggtt catggcagcg agctacaaaa cgtatacgtg aaggcaaacc
     6661 tgtgatgatt ggtattttga aagtacttgg cagtacatat ggcaatcatt gggtgacagc
     6721 gtacgcttat tttgaaactg aaacaggaga acgttattac aaagtacacg acaactgggg
     6781 cgattatcat aaagtcattc ctgccagttg gagtaacgga acagtttcac ttccttaaga
     6841 atttgctgaa attaacagaa aatcttggaa aaatatgtta aactatcgaa gaatagagaa
     6901 gttttgggga ggactacatg agtaataagt atcaggatga cacgttgaag atttttagtt
     6961 tgaatggcaa ccgtccatta gctgaaaaaa tcgcgaaagt gttcggaaca gaattaggta
     7021 aaagcgtcgt gaagcaattt agtgatggcg agatttccat caatatcgaa gaaagtattc
     7081 gaggggatca tgtgtatatc gttcaatcaa cgaatgcacc tgtcaatgat tactatatgg
     7141 aactactgat catgatcgat gcaatgaagc gagcaagtgc gaaaacgatc aatgtcgtat
     7201 tgccttacta tgggtatgct aggcaagacc gcacggcgaa acctcatgaa ccgatcactg
     7261 cgaaattgat cgctaacttg atcgaagaag caggggcaac tcgagtactg actttagatc
     7321 tgcacacggt tcaagtacaa ggattctttg atattcctgt agataattta tttacgatgc
     7381 cgttgtttgc tcattattac cgtcaattag gtctgacggg agatgacatc gtagttgttt
     7441 cgccaaaaaa cagcggtgtt cagcgtgcac gtagtctttc agaatatttg aacagcacat
     7501 tagccatcgt agatcatgca gatgatgctt caactgaggg tggttatgtg atcggtgatg
     7561 ttcaaggaaa gacatgtatc atggtagatg atatcttgaa cacaggagca acgcttgcac
     7621 gagcagcaaa tgtattaaaa gaaaacggcg caaaagaagt atatgcctgt gcgtcacatg
     7681 gattattgtc cgatcatgcg aaaagtattt tagacaatgc accaatcaaa gatatttgta
     7741 tcactgattc tgttttcaca gaagaaaatc gtcatccaga aaacttaaca atcgttacat
     7801 gttcagaatt aatgggtgaa gcggtcaaac gcatccatga aaatacaccg atgagtccgt
     7861 tattccgtct ggaagaaaaa gaattcgaat aaacaaaaaa aagagggaaa gaagttgtga
     7921 cagaagtgag cagcttcaag gagctgcgac aagacttttg tcacagctc
//
LOCUS       DAMYXM010000095         7886 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.099, whole genome shotgun sequence.
ACCESSION   DAMYXM010000095 DAMYXM010000000
VERSION     DAMYXM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.099"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..779
                     /locus_tag="PNS21_002524"
     CDS             <1..779
                     /locus_tag="PNS21_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDI5160182.1"
                     /translation="LKFSIIQTWIDLKYNAITTWNNIKQFFKDTWKNIKDTAYNTWIS
                     IKNTMINTWNNIKDSFWNIVTGIVNSAENAWTNLKNGVSNAINRVKEIFNSLREVNLF
                     EIGKNIIDGLINGVKEKWNALKDTIKGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIV
                     QGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVNGISHLPITQTKQNISASETKEIHLHL
                     NVYGDLPDSMVRKITTKIKTELTRQMKRDAGAVGGTLYAT"
     gene            769..1509
                     /locus_tag="PNS21_002525"
     CDS             769..1509
                     /locus_tag="PNS21_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI5160183.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1506..4268
                     /locus_tag="PNS21_002526"
     CDS             1506..4268
                     /locus_tag="PNS21_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI5160184.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            4281..5189
                     /locus_tag="PNS21_002527"
     CDS             4281..5189
                     /locus_tag="PNS21_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDI5160185.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            5189..5809
                     /locus_tag="PNS21_002528"
     CDS             5189..5809
                     /locus_tag="PNS21_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160186.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            5813..6262
                     /locus_tag="PNS21_002529"
     CDS             5813..6262
                     /locus_tag="PNS21_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160187.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            6262..6756
                     /locus_tag="PNS21_002530"
     CDS             6262..6756
                     /locus_tag="PNS21_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160188.1"
                     /translation="MADIVQLKEDGVAKYLKTHVKAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            6770..7201
                     /locus_tag="PNS21_002531"
     CDS             6770..7201
                     /locus_tag="PNS21_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160189.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            7203..7340
                     /locus_tag="PNS21_002532"
     CDS             7203..7340
                     /locus_tag="PNS21_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDI5160190.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            7377..7670
                     /locus_tag="PNS21_002533"
     CDS             7377..7670
                     /locus_tag="PNS21_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160191.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            7667..>7886
                     /locus_tag="PNS21_002534"
     CDS             7667..>7886
                     /locus_tag="PNS21_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDI5160192.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGN"
BASE COUNT         2853 a         1093 c         1654 g         2286 t
ORIGIN      
        1 aattgaagtt ttctattatt caaacttgga tcgatttaaa atataatgcc attactactt
       61 ggaataatat taaacagttt ttcaaagata cttggaaaaa tattaaagat acggcataca
      121 atacgtggat ttctataaaa aataccatga ttaatacatg gaataacatt aaggactctt
      181 tctggaatat tgttactgga attgttaatt ctgctgaaaa tgcatggaca aatctaaaaa
      241 atggtgtttc aaatgctatt aatcgggtaa aagaaatctt taattcgtta agggaagtca
      301 atttatttga aattggcaag aacatcattg atggacttat aaatggtgta aaagaaaagt
      361 ggaatgcttt gaaagatact atcaaaggta ttgcaggtaa tatcaaaaat tggattaaag
      421 gagctttaga tattcattct ccatcaagat ggatgcgtga tatggttggt aaaaatattg
      481 ttcaaggtat tatcgttggt attgataaag agcaaagtaa gttagatcaa accatgacaa
      541 atttagtaaa aacaccgtct gttcaacctg tagtaaatgg aataagtcac ctaccaatta
      601 cacaaacaaa gcaaaatata tctgctagtg aaacaaaaga aattcattta catttaaatg
      661 tttatggaga tttacctgat tcaatggttc ggaagattac cacaaaaatt aaaacagaat
      721 tgacaagaca aatgaaacga gatgctggtg cagtaggagg aacattatat gcaacttaag
      781 agaggacaat tttttattaa tcaacattat tcttctgaat ttaatgtgta tattcaaaac
      841 aggcctgctt ctgtttcagc tagtcgagta attgaattga gagagcgaga aggaaatgat
      901 tctatcatta ttgataaagg ctattacaga aatgtgacta gaaagattga gtgttactat
      961 aaagcacctt caattgatgt ggtgcaggag tgggaagatc gaatcactga atggttagat
     1021 atgtcgtctt atagtgattt tattctttac tatgatgaac aatatattta tcaagctgta
     1081 gtaatagaag gacctgaatt taaaggaaca agaaaaacag gaaatattgt cccttttgaa
     1141 tttacagtga gcattcgacc atttaaggaa aattacaatg gtagatttac tattcgacaa
     1201 acagaaactt ttgaaattta taacccagag aagtattctt caaaaccgct tattaaattg
     1261 agtggttctg gagatgcttc tttttatatt aataaggaca aatatgattt gaaatcgttg
     1321 gacagagaat tatttataga ttctaaatta gaagaggctt accgaaaatt agatggtaat
     1381 ttagaacatc aggatcaagt cactttattt ttagattttc catttttata tcctggaaaa
     1441 aatgaaatta aatggacgaa aaatattcat tcatttgaaa taatgcctag gtggtggaga
     1501 aaagtatgaa accaagaata tatagtccta ctgaaacaga tttctcaacg aatggcttag
     1561 gaattttaaa ggattgtaca agatgtgaaa tatatgaagt agctaatgga aaatatgaat
     1621 tagagttgga ttatccttta ggaactagat ttgatgaata ttttgaaaat gactatcaaa
     1681 taaaagcaaa gccaaacgat caagaagagt atcatatctt ttttattgat gataaagata
     1741 tagatacttt tttaaataca gtaactattt atgctcagag tcgtacaaat cgacttggaa
     1801 gacgggtagt cactcttgcg gaagtagact ctaaaactgg tcaagaagcg atatcaatta
     1861 ttgaaaccaa aatggataaa aaatctgaca tacgacttta ttctgatatt acggccgttt
     1921 ctagcacaat ctttgaagcg agaaatgttt taaattgtat tgctggtgaa caaggatcat
     1981 tgcttcaata ttggggtgga gaaattaaac gtgaaccatt taagctttct ttgttaaagc
     2041 gaagaggtcg caataatgtt ggaacgattc ggtatggaaa agatatgtct ggtttaaagg
     2101 tcaaattaga ttggacaggt ataaaaacaa gacttattcc atatgctgat cctcaaagtg
     2161 aggtaggtac gactaatcga atttatggtt cgccagtaga tagtgcctat attaataact
     2221 atcctgatgt gtatacagag catgttcagt ttacagaaga acaaggagta aaagacatta
     2281 agagcttaaa taaaatagct aaaaattact ttaaaacgat caatcctggc tgtgataagc
     2341 ctaaagtttc tattacggtt gaatttgata agttgactga tagtgaagaa gcgaaagaat
     2401 ttgcgaagat tagaaattat ggtctgtttg atacatttaa aatatatcat aaaaaatata
     2461 atatttatct tgaatcaaag gttagtggac tacaatacga ttctttatct gaaaaaactt
     2521 tgaaattaga agcaggagac attcaagttg ctttttatca gcaacaagct gtaactattc
     2581 aggataaatt aaaagattat gcaacgaata attatatgag tgattttaat gattatgttt
     2641 cttcaatgat tacaggtcaa ggcagtgcag gaggatatgt aagtttatgg ccaaaagaaa
     2701 aaccttccaa catctttatt atggataatc cagatttaga aaaggcaaga gaagtactaa
     2761 gaatgaataa aaatgggatt gctttttcta agaaaggatg gcgggggcct tttacttcag
     2821 cttggacatt agatagtata tttaatgcca attttataca aactggatta atcaaagcag
     2881 atatctttca aaattctttt aacaagactg gagatgtatt gaaattagtt aatggactac
     2941 tccaaatttg gaacaacaaa aagaaaataa tggagttaac taaaaaagga atggaatttt
     3001 ggaattctaa gggttcaatt ggaacgattg gaacaactga ttctgctggt aatccttttc
     3061 ctggggcttc tactccaacc cctattgaag ataattcttt agttattcgt acaaatggag
     3121 acggcaaata tattttgatt tctcctaaag taggaaaagg attagtttta ttaggaaatg
     3181 gtaaagcaat ttattttgga gacttagatg tacaaggaaa acttacagtt aaaggaaaag
     3241 aaataattcc tggacaaggt ggtggtccat cgggaggtgg agaatctact ggtgggtatc
     3301 ctagtgaatt aaaaacagat gcagaaaaga gagcttggag aatttacgat attttgtgta
     3361 ataacggatt tacgaaacaa tctgcatgtg gaatattggg gaacattcaa caagaaacag
     3421 gaggaacttt tgatcctgat actgttcaaa taggtggacc agcatacgga ttagttcagt
     3481 gggatggttc ttcatatcct cttgttggtc cagcaacatg ggatggaaaa gtttatgttc
     3541 aaaacttatt taatgctgca ggtattaaag aaccaataac gagcttagat gcacaagttc
     3601 gattgcttat ttggacattt acaaatggac aatggatggg cgtagtacaa cctacgacgg
     3661 ttgatggatt taaggcctgt actgatccaa gacaagcagc atatgctttt gaacgaaact
     3721 atgaacgtcc ggcagcgaca catcctgaac gtcaagatta tgcagttaac tggtataaca
     3781 aatttaaaga tttaaaacca ggaggagcta ctggagaagc aggactaaaa catttagagt
     3841 ctttgattgg acaacgaatt ggcaatggtc agtgttatgg cttgtctgca gaatattcag
     3901 gatatctggg tggttgtggc atgggtgctg gaacaaaata tggtttaact catgtgatag
     3961 gaaatacttc tgcagcatct gatattggta ttgcctatga ttggtctgct gttggttgga
     4021 aagtgattca aaaccctaga tatgatcaat tagtagttgg tgcaattatt aattgggcaa
     4081 gaggtggaca agtgggttca tggtttgcag acggaactta tggacatact ggcgttatcc
     4141 gaggcttagc taatggtcgt atgcaaactt atgaacaaaa cacagagtta ggtatgattt
     4201 gtggaaagtt agatcgccag tattatagtg caagtgcaat ttcttccatt gtcataccac
     4261 caaaataggg aggtgattta atggcaaagt ggaatgtcat attaagtaca acagaaccct
     4321 ataattacgt ggggatgatt caagttcgac aaggcaataa aaacactgaa gttatggaag
     4381 ctacaattgt cgaaaatggt cttccatatg atttatcaga atgtaaggta tattttgaat
     4441 cagttgttgg cggaaaatat ccagtccaat tagaaacaag aattgtggat gctaaaaaag
     4501 ggaaaatcaa atatattttt gataaatatt ccatgcagtg tttgcatcga caaacagcca
     4561 atttcattat atttaaagga gaagacttga ttggaacaac tcaagacttc tcctattttg
     4621 tcatcaatgc tgtttcaaaa acagaaggag aaatgggttc ttattggcaa tcaattgaag
     4681 atttaattgc tgatatgaca gattttatta atgaaaataa aggcgatttt acggactgga
     4741 tgaatgaaag gaaagaagaa tttgatcgct ggagagaaga acaggagaat agtttccaag
     4801 attggagaga aggacaagaa tcagattatt tgtcatggtt tgaatcaatc aaggatattt
     4861 tgaagtctat cgatccaggt ggagtaatgt tagccgaatt aatggatgca cgtgtagatc
     4921 ttcaaggtgt acgccatgaa tcgatttctg aacgtttcct atccgatcta aattatttgt
     4981 atcaaaaaat gaaagcagca cttttcacga ttgaatacgg tgaaattgaa gtgactgata
     5041 ttcttcaaga cgatctcttt tcagataatc acgaagtcga aaaagttgga actgtagaat
     5101 tcccgattga agaaggagcc ttgatcatcg caaccgttga tgatccaaag caaaatgtgt
     5161 tcactcttga gaaagtcggg gtgatctaat ggctaaaact aaacgaatga tggaaacgga
     5221 tgaaaaaaca ggtgtacaac gccaattctt tccaatcaca catgcttccg cagttcttgg
     5281 gttagaagaa ataatagcag gagaagcaac agttttatct gttaatggaa aaattggagc
     5341 cgtcgtcatt actaaagaag atttgggatt agagaatgtt ctcacagaat taccctatgc
     5401 aagtgaagaa gatgacggca ttatcacggc agaaatgtat caaaaaattt taaacagtgg
     5461 agaaggtgac tacgtgttgc cagttgccac tatcgaccgt ttgggtggca taaaagttgg
     5521 tgaattattg acgattgatg agacagggaa agtctctgca gtcagacaat ctgatgtcaa
     5581 tttttcgctg gagttaaaag aaaaactcga ttcactgaaa aattatactg ctggagaaaa
     5641 catcactatt gatgaagatg gaacaattag ttctacaggt ggtagtggga ctggtggtgt
     5701 caatcagtca tatgtagatc aaaaattcca agaagctgta aatcaagcag aaaattacac
     5761 aaacgaaaga attccaaatt ttacttttga aaaaattggg gaggtataga gaatgacaga
     5821 tatcgtaaaa ataaaacaaa gcaatgtaca ggtttatcca caaactcatt ggaatgctat
     5881 agaaggcaaa ccaacaacgg tgaagggaga taaaggcgat cctggccaag cagcaacaat
     5941 taccatagga accgtttcta gtggttcaac cgcttctgtt acgaatgtag gaacatcatc
     6001 tgcagctaga tttaattttg ttttaccaaa gggagataaa ggtgatcctg gaataaatgc
     6061 cacaactaca gctgtagcta caactacagc aaatgggttg atgtcgtcca cagataaaac
     6121 taagttggat ggaattgcag ctggagcaca gaaaaatcca ggtaatgcta caactacgac
     6181 agcaggttta atgtcagcaa ccgataaagt gaaacttgat ggattagcca atattacatt
     6241 tgagaaagtg gggacggttt aatggctgat attgttcaat taaaagaaga cggagttgct
     6301 aaatacttaa aaacgcatgt aaaagctatt gatggtaaag aagcattggt gcagacagat
     6361 ggagaccaag ccattgcagg acataagaat ttttcaggtt ctgtaacaat caataataaa
     6421 cgtgttttga cgactgatga taacagatat gaagtggtaa atctggttgt cactaacggt
     6481 aatacaggga cagcaaagct ttatcgtgaa ggaaaaacag tttctatata ttttgtggct
     6541 ttaaacggaa aaagtagtgg cgggaatgat tcggttattt taactgttcc agaaggctat
     6601 cggacaccaa ttagttttga acaactggtt gggtcaatag accgttctac tttgaacagt
     6661 gctcaactat ctattggcgc agacggagcc attaaatgga gaagaaattc aagttatgga
     6721 tcagcttatt catttgttat cacttattca atttaaggga ggaaatctaa tgaaagtagt
     6781 ttacaaatca atcaagcctt acggattcga gcaaatcatt ttgaacaatc aagaaaatat
     6841 ccctgaaaac tgtacagaga ttaaaccacc agttcctaac tggagaccaa gatttgattt
     6901 tgataaaaaa cagtgggttg agttagctac tgaagaagag aaaaaaggga atgctgttga
     6961 tagcgtagat gaattggcta atattaaggc attatacgaa acgctgaagg cagaaaatga
     7021 tgaattgaaa cagctaaatt ctaaagcaat gctaaacaat gtagcaatca agcaagaaaa
     7081 tgtcttattg aaagaaaagt cagaaagttt agcgcagttg aattcaaaaa cgatgcttgc
     7141 ttctgtacaa aataccaagg aaatcgaaga aatcaaaaaa caattacaag gtgggaagta
     7201 acatgtattc atatgaagat atcaaactaa tgtatgactg gggctgtttc actaacgaac
     7261 aagtaatggt tttcgttcca ttatgcatta cagacgaaga agcagataaa atcattaata
     7321 aagataagag cgcatcttaa ttgatgcgtt tttatttaag gtaaaggagt tgtcacatga
     7381 ttaatttagg ggaatgggga atgatagcag gatcaataac cgctatcgtt tctttgattt
     7441 tattagtaat tagaccgatt gctgcatctt tctcgaaaat tactgagact ctttcaaaag
     7501 taagccacaa tttagatttg ctgactaaag atttagaatc gagcaaatca gatcgattga
     7561 tgattcatga agaactaaag aaacacgatg aaagattaga tacacataca gaaaaattgg
     7621 tggaacacac gcaacaaatc aaaactttgt ttagggagag aagaaaatga ataataaaac
     7681 gttcgaagta ctaaaatggt tcgcactggt aattattccc gcactagcta ctttcgtggg
     7741 gttagttggt aaagcgctca attggcagta cacagatatc tgtgttgtca tcattactgg
     7801 ttttggcgcg tttttaggga gtgtgttggg tgtatcaaat cgaacctaca aaatgttctc
     7861 ggctgaaagc gaagaaggag gaaaca
//
LOCUS       DAMYXM010000096         7824 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.100, whole genome shotgun sequence.
ACCESSION   DAMYXM010000096 DAMYXM010000000
VERSION     DAMYXM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.100"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..1181)
                     /locus_tag="PNS21_002535"
     CDS             complement(<1..1181)
                     /locus_tag="PNS21_002535"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDI5160193.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQ
                     YRQLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQ
                     DLLTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLEN
                     L"
     gene            complement(1360..2133)
                     /locus_tag="PNS21_002536"
                     /pseudo
     CDS             complement(1360..2133)
                     /locus_tag="PNS21_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            2154..3356
                     /locus_tag="PNS21_002537"
     CDS             2154..3356
                     /locus_tag="PNS21_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HDI5160194.1"
                     /translation="MCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNT
                     LYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAK
                     GLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYE
                     SAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDY
                     QLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEM
                     ILNDVNNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTE
                     VNINEYSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVT
                     EYIKNYFN"
     gene            4089..5057
                     /locus_tag="PNS21_002538"
     CDS             4089..5057
                     /locus_tag="PNS21_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDI5160195.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            5068..5883
                     /locus_tag="PNS21_002539"
     CDS             5068..5883
                     /locus_tag="PNS21_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HDI5160196.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            5951..6661
                     /locus_tag="PNS21_002540"
     CDS             5951..6661
                     /locus_tag="PNS21_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI5160197.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            6697..>7824
                     /locus_tag="PNS21_002541"
     CDS             6697..>7824
                     /locus_tag="PNS21_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDI5160198.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         2598 a         1237 c         1589 g         2400 t
ORIGIN      
        1 tggagatttt ccagtcgtgc attcgaatac ttcttcagta atgccagccg aatccctttc
       61 tcgtatttga aaaaggcttt tttgatatgg tgcagttcca atggaacttc tccaggcatt
      121 gctcgaagca gctggaaaaa agtatctgga tctttttcct gatacgcagt aagcaggtct
      181 tggtagtaac gataagctgt tttaagttca ggagaaatct ttagaaggcg gtcaactact
      241 tctgtttcag tcaatagtct ccaattgaaa ttgcgtcttt tcgttagtgt attgtaatct
      301 aaacgatcct ctgattttaa aagtaatttg aataaggatt tcaattggcg atactgttta
      361 gcttgtattg ggtctttttg atcaagtgtt ttcatcacac gttttcgaac cgcatcgaag
      421 aagcggttca gatgttgaac aatgtgaaac cgatcgacga tcacttgtgc atttggaaaa
      481 cactctttaa ctagtgagaa ataaggagcg ttcatatccg ttacgatgta atgtacaccc
      541 aacctcgctt tgagagaaaa acgcatgaaa aaagcaacga gatctttttt acggcggcta
      601 ggtaagatct caaacagttt cccggtctca ccgtccatac aagaaaaaga aaatttaccg
      661 tgccatgtag aaagagaacg aaactcatcg aataatagac accgaggtaa atactcaaga
      721 ttctgacgga tatctgatga caaggcggac aatgtccgct gaacagttgt cacagaaaca
      781 tcgcaatgtt taccaattgc ttttatagat tgacgctctg tcaattcttc taagatcttg
      841 taatgaatct gcttagaaat aaagcagaat ggggtaacga gatacgtccg agcggtaaaa
      901 tattttttac atgctcggca gaggtatcgc tgtttcttca accgaagata ggtccgttta
      961 ttaccgctac gattcaggag aatcatagag atcttcttgc catttttaac gatttgcttt
     1021 gtagaatctt tacaacacgg acaacgtctg taggtacgga tcagagttcc ctcaataacg
     1081 atactttttc tgcttttata aaaagtttct tcaaaggatt gaatttggat atttgagtct
     1141 ttaatagtaa agagttgttt gataaaatga ttcatggaca tacaagaatt cccccagaat
     1201 gatgttgtag gagactctat tctaccggaa attttgtatg tctttttgtt tttttacaaa
     1261 aaaactagga cagatagaaa atttccatca gtcctagaaa ttatagagcc aataaaactt
     1321 agtccgatat gcggcgcgtt ctgtgcgccg catatttttt tagttttcaa agatcagacc
     1381 ttgttgtaaa tagatccgtg cccgaaagtt ataaaagttg cgataaccaa acgcaattcg
     1441 tttaatcagc ttgatcttgt tatacagccc ctcagtgacg ccgtttgagt gggacacctt
     1501 aaaggcattt gaaatacccg attggtagcg atggaagatc gtgaatttct ttttaaacga
     1561 ctgaggtaac gtagatgaaa ttgttttaag ggtttcttga aagcctaccg tatctcgttt
     1621 aacgaaataa tagcgtaata attgacaaaa atgatacgca gttcgcaatt cttcgttgta
     1681 gcttaacagc tcattaacga tttctgtttg actaattggt cgtttaaaca gtcgatgata
     1741 atgccgtttg agtgcctccc atgtcgtgga atctttgagt aagagtttcc aatatcgctt
     1801 gagccgacgg tacttcttct gattttcaga gtgtgaggtt cgaaaacgat tcatgatctg
     1861 gacacgtaat tgattgaagc tgcgattgat gtgttggaca atatgaaagc ggtcagttac
     1921 gatctccgca tttggaaaga ccgttttaag caactgacaa tagctggcat tcatgtccat
     1981 cactaggtac ttgaccttta aacgggcctt tctaggaaag cgcaggaagt attgagttag
     2041 tttgtaaagt ctgcgatctt caagggtcgt aaatagctga ttggttgtcc catcaacaca
     2101 aataaaactc atggaaccag aagtggcaac attgtgtact tttctttgca atcttgtgta
     2161 caaaactatt gcaaaacaca gcgcataaga atccttacaa aacagtagat gaagcaaccc
     2221 aattagatct tgttatagtt gttaatcagc tcttaacggg ttacgattca aaatgggtca
     2281 acaccctata cttagataag tcacttgaat atcagaacat aattcaggct ttttcacgca
     2341 caaatcgtct taatggtcct gaaaaaccgt tcggaaccat tcgttattat cgtaaagcac
     2401 atacgatgga acagaacatt gaagaggcta ttgagttata ctcaggcaaa caagcaaaag
     2461 ggttatttgt tgaaaaatta tataataact tagaaaagtt gaacgatata ttcgaagaaa
     2521 ttaagtccgt atttaatatt tctggcatag aaaacttcag tcatttaccg gaggttccag
     2581 gggaaattaa gcaatttgta aatctttata atcaactaat ccattgttat gaatcagcga
     2641 tgatacaggg attcacatgg gcaaaatcga cttatgaaat ccaggattat gaacctataa
     2701 cagtagtaat cacagaatat gaatttaata tcttggcaca acgttacagc gagatagaga
     2761 gagaacctag agacggaaat gatagtatag cgtatgacat tgattaccaa ttacctgaag
     2821 gtagtcgtag tcgtattaat tatgagtatt taaacacaaa cttcaaaaaa tacatgaata
     2881 taaaagaaga acgtgattcg aatttaattc aatcattgtt aaacgactta catcgcacat
     2941 ttgcgcaatt accagtggag ttacagccat atgctgagat gattctaaat gatgtaaata
     3001 atggggaatt agttatcaaa gaaggatggg aatttactga ttacctcaat gaatatcagc
     3061 ttagtgaaga ggacgatttt atccaagatt tagtggattc aaccaatatt aatgaggcat
     3121 tattacgtga gatgttggat ttacgcttaa ccgaggtaaa cattaatgaa tatagtcgat
     3181 ttgataaatt aaaatcaagt gttaacgtag gtcatttcag tggatatatc gagaaaaatt
     3241 taggtaaaat ggtaatacca atcaaggtaa atatgctaat tgacagatta ttgcgtgcat
     3301 ttttattaga agatgtattc gatgttacag agtacataaa gaattacttt aactagagta
     3361 cacctacatc ttatagtaca taaaaaaatt catctttttt agtttaataa ttcaccaaaa
     3421 aggtgagtaa tgccattttt gaaaattgag cttgttttat gtctacatct gtatccgtat
     3481 ataatccaaa ggattataaa ctcagaagag tctttatttt ttctgaatat aactactgta
     3541 tcaaaaacaa tcgattatga gacaaaacaa aatgacctct tttatttaag aaaggaggtc
     3601 attttttgta tcataaattg tattaaaatc aatgtatcac tttttaatca attttaagac
     3661 cataaattgc gtactaaaag aaattctgca attcaaaata aagcatttta agccattttc
     3721 aaatttaagt gcacccatct acccgtcaaa tcactttatt ttccactcca gtagtttcct
     3781 aattcaactg tgtgcaaaac gatttcttct aaacaaatca agacaaactt ataaaccttc
     3841 atcccagcac cataattatt taaatcatag gcatttttcg aaattagtat atgtactcaa
     3901 gtttcactgc agattattta tattttttag agaaataaat ttttcctatt gacaaacaca
     3961 aagaaagcgg atacaataca attatgtcac atgacgttat atgacgttat gtaatatctt
     4021 tacatttaat cagttacttt tattttggat aaaattaaca tttttaaaat tttaaggagg
     4081 aaatgttgat ggatgtagca aaaacggtta acgcaattgt agaaaaaaat gatggagaga
     4141 tttctggaat ttatttggtg gcttgtggcg gttcgctggt agatatgtat gtctcagatt
     4201 attttcttaa agccgaagca tcgaagtgta ttacaggctt atatacagcg aacgaattta
     4261 accatgtaac cccaaagcaa ttagatgata aatctgttgt cattctatgt tctcatggag
     4321 gaaatacagc agaaacggta aaagcaggag aagtcgctca agctagagga gctgtgactg
     4381 ttggtctgac tcataataaa actgcggatt tattaaaggt ttctgatcat agtttccttt
     4441 acgaatgggg agatgatgca aaaatagtca ataatcctat ggccatcatt cttgatttaa
     4501 ctgtatcttt ggtcaatgtt accgaaacat ttgaacatta cggtaaattc agagaaggca
     4561 tgaaggttat cgataccgta attgaaaatg gcattaagca ggtgcaggat cgtattaaag
     4621 tgtttgctga taagtacaaa gatgaaacaa tgttttatat cttaggtagt ggaccatcat
     4681 atggacatgc ttatggtttt tcaatttgct ctttgatgga aatgcaatgg cttgattcaa
     4741 cttctgttca ttctggagaa ttcttccatg gaccttttga agtaacagac aaagatacga
     4801 attttattct cttgatgagc attggacgca ctcgtgcttt agatgaacgg gcaaaacgat
     4861 tccttgataa atatgcagaa agagtagaaa tcgttgatgc aaaggaacta ggattggata
     4921 tgatatcaga tgaagtttct gaattcttca atcccatttt attctacagt attctttcag
     4981 aatatcgttc agctttagca gatgcacgca atcatgattt agatgttcgt cgctatatgg
     5041 ggaaagtgga gtactaggag gattaaaatg aaggtattag gcttaggtga taacgtcgtc
     5101 gataaatatg aaaatctggg aattatgtat cctggaggaa attcattgaa ttttgcagtt
     5161 tttgcaaaaa aactgggaca tgaaagtagc tttatgggag tttttggctc agatgaggag
     5221 gcagctcatg ttttatcagc aataaaaaaa ataggactgg acaacagtca ctctcgcttt
     5281 gaaaaaggcg agaatggctg tgccagagtg aagattgatg agggcgaccg gattttcctt
     5341 ggcagcaatt cgggaggtgt tactcgtgaa tacccgattc agcttactga agaagacaga
     5401 gaatatttaa accagtttga attgattcat atgggattat atagccatgt caatcatcta
     5461 ttagaagagt tacaaaatgt ttctggaaag atttcttatg atttttctga cgactttacg
     5521 gaagaagagg ttcagcgggc aattgataaa gttgattttg gcttcttttc tatagaagac
     5581 ttttccgatg aagaagtaaa gcagtttttg atgaagcatt tttgtgagcg aaatgatgta
     5641 ttgattgcaa cccgaggaga aaaaacggct attggttatg atggagcaaa gttttatgaa
     5701 gtgaaccccg tgctagaaga accagtcgat acaatggcag cgggagattc tttcttaaca
     5761 gctttcctac tttattttct cgaaggggac gatatagtca ccgcgatgaa aaaggggaat
     5821 gaatttgctg ggaagtcttg tctggttgac ggttcgttcg gctttggtat tcattacgat
     5881 tgagcttgtt ttaaattcct gcatttgata acctaaagaa agaaatcccg gtaaagtgga
     5941 ggttaaaaaa atgctggata caaatgcaca attaccattg tacttacaat taaaagaaat
     6001 attaaaaaat aaaatcaata caggggagta tcccgagaat acgaaaattc ccgctgaatc
     6061 agagctgata gaaagttatg aggtcagccg aatcacagtg agaaaagcag tagaagaact
     6121 ggtagaagaa gggtacctac ttaaaaaaca agggaaagga acttttgtca accggcataa
     6181 agtttttcgt aaaattgaat atgttatggg tttcagtgag agttgtaaat ctaatggttt
     6241 tacaccgact agcattgttt tggaacgcaa gatcattcca gcaactgaag ggatggcaaa
     6301 aaaactcgat atcgctcaag gtgatgatgt tttatatatc cagagaaagc gaatggcgga
     6361 taacatcccc attatgttag aaaacaatta tttttcttat gaaagatata gtttcttaat
     6421 ggaggaagag ctgactgatt ctttgtatga agtacttaaa gtccaaggta ccttagccat
     6481 caatcctggg gaaactgcac ttgaaattgt cacagctgat gaccaaattg ccaaaataat
     6541 ggaagtagct gttgggacgc cctttttctt tatggatact ttaatcaacg atcaaaacaa
     6601 cataccaatt catgtaggta atcaatatta cctgggagaa gtatatcgtt tttacttatg
     6661 atttcctgtt ttgatttcaa ggagtagatt ttatttatga agcaaatatt gcggtcaaaa
     6721 atgatttttg atggagagca gccacctttc aaaggttata ttgtaatcaa tgaagggatc
     6781 attgaaaagg tggaaaacaa ctggaattac agtgatctat taaatgaaaa cacggaatta
     6841 attgcttatg atgatcaatt cgttatgcca gcaattcatg ataatcatgt atttttcagt
     6901 ggttatttgg ctatgaatgc tggcatagat ttaagcgata catcttcagc taacgaggct
     6961 gtagagcgta ttaaagctgt aatgaaccat aaaaaagcag atgaaccaat ttatgctcat
     7021 ggctgggatg ctgccctgtg gaaaactgac ccatcagaag agctattgaa tgaaataaac
     7081 cactttggtc caatcacagc gatcgacaaa aatcgcagcc attgttggat gaatgctatt
     7141 gctaagcaga cttatggttt tacagaaaaa gaaaccagtg ctgaaagtcg agtattatta
     7201 attaaggaaa tgctagcaaa caaaaagctt ttagcagagg tttacgctga atttgaagca
     7261 ttactgcttt caaaaggtgt tatatcaatg aaagaaatcg tttttgatga cgcagaattt
     7321 ctttctaata tacctcagag gaaaatactt agtaacctat atgtgcaggc agtccaagaa
     7381 cctctacagc atgaacttct atatgagtat aaggaaaaag ttttccctga aaaggtccgc
     7441 tttggtggag tgaagatcat ggtagatggt gttgttgcgg atagaagtgg agatatttac
     7501 ggtgtttatc agtcaggagt agcttcaccc gagattgact atgaggcaat taaagcagaa
     7561 gttgctgcat tcaatgaagt agagattcct tgctgtttga caacggaagg agataaagcg
     7621 ggttataaag ctgcgtcaat tcttgtcgaa tttggaaagc ggttacctga aggcgttttt
     7681 aattcgatta gtgatttgga aatggttact caaaagacag cagaaaaaat gaacgaagga
     7741 caagtagtag cagaggtata tccacaaatt ctcggcttga atcaaacgtt agaagaggct
     7801 tacatgtcaa ctgttataaa aaat
//
LOCUS       DAMYXM010000097         7599 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.101, whole genome shotgun sequence.
ACCESSION   DAMYXM010000097 DAMYXM010000000
VERSION     DAMYXM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.101"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(942..1355)
                     /locus_tag="PNS21_002542"
     CDS             complement(942..1355)
                     /locus_tag="PNS21_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDI5160199.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="PNS21_002543"
     CDS             complement(1811..2296)
                     /locus_tag="PNS21_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160200.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2650)
                     /locus_tag="PNS21_002544"
     CDS             complement(2435..2650)
                     /locus_tag="PNS21_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDI5160201.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2874..3674
                     /locus_tag="PNS21_002545"
     CDS             2874..3674
                     /locus_tag="PNS21_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI5160202.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3693..5561
                     /locus_tag="PNS21_002546"
     CDS             3693..5561
                     /locus_tag="PNS21_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDI5160203.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5554..6045
                     /locus_tag="PNS21_002547"
     CDS             5554..6045
                     /locus_tag="PNS21_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDI5160204.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6033..6587
                     /gene="srlA"
                     /locus_tag="PNS21_002548"
     CDS             6033..6587
                     /gene="srlA"
                     /locus_tag="PNS21_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDI5160205.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6605..>7599
                     /locus_tag="PNS21_002549"
     CDS             6605..>7599
                     /locus_tag="PNS21_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDI5160206.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2236 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     2701 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     2761 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     2821 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     2881 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     2941 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     3001 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     3061 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     3121 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     3181 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     3241 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     3301 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     3361 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     3421 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     3481 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     3541 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     3601 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     3661 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     3721 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     3781 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     3841 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     3901 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     3961 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     4021 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     4081 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     4141 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     4201 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     4261 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     4321 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     4381 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     4441 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     4501 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     4561 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     4621 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     4681 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     4741 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     4801 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     4861 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     4921 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     4981 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     5041 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc
     5101 gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt
     5161 atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat gtggatgaag
     5221 aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat
     5281 ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa
     5341 taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac
     5401 ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat atcctatttg
     5461 aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt
     5521 taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt
     5581 ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc
     5641 aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg
     5701 aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa
     5761 gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg
     5821 ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg
     5881 acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa
     5941 aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa
     6001 agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg
     6061 gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg
     6121 taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag
     6181 accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt
     6241 taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc
     6301 ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca
     6361 atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag
     6421 ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac
     6481 tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac
     6541 agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt
     6601 ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat
     6661 tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc
     6721 aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac
     6781 atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg
     6841 tggaatttat cctaaaaaag ggataccaac catcaatatc gtttccacag gaaaaagtgg
     6901 cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat
     6961 acagccacta atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa
     7021 aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg
     7081 gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag
     7141 agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt
     7201 gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt
     7261 agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc
     7321 tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga
     7381 gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac
     7441 tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac
     7501 agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt
     7561 tatcattgct tgggtagcaa gttttggatt atatagctg
//
LOCUS       DAMYXM010000098         7374 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.102, whole genome shotgun sequence.
ACCESSION   DAMYXM010000098 DAMYXM010000000
VERSION     DAMYXM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.102"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            1..816
                     /locus_tag="PNS21_002550"
                     /pseudo
     CDS             1..816
                     /locus_tag="PNS21_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
     gene            832..981
                     /locus_tag="PNS21_002551"
     CDS             832..981
                     /locus_tag="PNS21_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160207.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            1062..1658
                     /locus_tag="PNS21_002552"
     CDS             1062..1658
                     /locus_tag="PNS21_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDI5160208.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            1671..2570
                     /locus_tag="PNS21_002553"
     CDS             1671..2570
                     /locus_tag="PNS21_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDI5160209.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKDGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            2573..2704
                     /gene="ssb"
                     /locus_tag="PNS21_002554"
     CDS             2573..2704
                     /gene="ssb"
                     /locus_tag="PNS21_002554"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI5160210.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            2726..3058
                     /gene="ssb"
                     /locus_tag="PNS21_002555"
     CDS             2726..3058
                     /gene="ssb"
                     /locus_tag="PNS21_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI5160211.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            3080..3337
                     /locus_tag="PNS21_002556"
     CDS             3080..3337
                     /locus_tag="PNS21_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160212.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(3496..3618)
                     /locus_tag="PNS21_002557"
     CDS             complement(3496..3618)
                     /locus_tag="PNS21_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HDI5160213.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            3808..4032
                     /locus_tag="PNS21_002558"
     CDS             3808..4032
                     /locus_tag="PNS21_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160214.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            4475..4681
                     /locus_tag="PNS21_002559"
     CDS             4475..4681
                     /locus_tag="PNS21_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HDI5160215.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            4681..4932
                     /locus_tag="PNS21_002560"
     CDS             4681..4932
                     /locus_tag="PNS21_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160216.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            4948..5103
                     /locus_tag="PNS21_002561"
     CDS             4948..5103
                     /locus_tag="PNS21_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160217.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIE"
     gene            5113..5385
                     /locus_tag="PNS21_002562"
     CDS             5113..5385
                     /locus_tag="PNS21_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160218.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            5830..6009
                     /locus_tag="PNS21_002563"
     CDS             5830..6009
                     /locus_tag="PNS21_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160219.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            6068..6424
                     /locus_tag="PNS21_002564"
     CDS             6068..6424
                     /locus_tag="PNS21_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160220.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         2657 a         1193 c         1494 g         2030 t
ORIGIN      
        1 taaaggcaat cgaaaaggtg ccgctagatc taggttggaa gaagttattt ggaaaagaga
       61 gcgacggaaa agagaaggaa gaagagccgc tacttcctaa agtgacaaaa ggagaaatgg
      121 tgacggtgga tctacaagtt cttgaaaaag aaaccaagcc accacaacct tataccgaag
      181 gtacgttgat cactgcaatg aaaacagctg ggaaaaccgt ggatagtgaa gaagcgcaat
      241 ccatattgaa agaagtggaa ggaatcggta cagaagcgac acgggcgaat attattgaaa
      301 ccttgaaaca aaaggaatat atcaaggtag agaaaaataa gctcgtggtg acgaataaag
      361 ggattttgct ttgccaagcc gtggaaaaag agccgctgct tacgagcgcc gaaatgaccg
      421 ccaaatggga aagctacttg ttaaagatcg gtgaacgaaa aggaacacaa accacttttc
      481 tagccaatat ccaaaaattt gtctctcact tattggaagt cgtaccaggg caaatccaat
      541 ccactgattt tggatcgacg ttacaagaag tgaaagcagc cagtgaaaag caagaagccg
      601 cgcgtcattt aggaatttgt ccaaaatgcc aggaacaaga agtcttactc tatcaaaagg
      661 tggcagcttg tacgtctgaa gcctgtgatt ttaaaaggtc agaagggttc atttgaagca
      721 acgattgttt taaaagaaga cttcactaca ggctttgaat tttctgaaaa gaaaaaaacg
      781 aacttcaaga aaagaacacg ccgcactgca aaataatgga gggttaatct tatgcgtaat
      841 accacacaca aacaacaaga aatcgaaaat caaaaagcca aaattcgttc agaacaggaa
      901 aaaatgcgtg ccagcaaacg aagactgcag caattgaaga accagcagag gattcaaaaa
      961 gaaatcgaat tagatcgata aaatcttcct aactattgta tacgtataaa cttatgttat
     1021 actgttttta taagtttata cgtatacaaa ggagggagaa aatgacaaaa gcaatggtat
     1081 acaagctgct agaagaattt aatggaactt tggctttgaa ggatgccaaa aaagcgggca
     1141 tttctcctgt aacgatcaaa cgtatggtag atcgagggga gctggatcga gagtatccag
     1201 gttttttcac cttacctggt caatttcctg atgaattgtt tatggcgcaa aaaaaatatg
     1261 aacgcggcat tatttcacat attaccgcgt tagatttgta tgatttaaca gatatgatcc
     1321 ctagacagat cgatctgact gtaccttacg gttatcacgt ttctgaaaaa ggattgaagg
     1381 aattcgcagt tgagctacac tatacaaaag cggaatggta tgaattaggt aagattgaaa
     1441 taaaaagtcg ctatgggaat cctattatag cttatgatcc agaaagaact ttatgtgaca
     1501 tttggaatcc ctggtatacc gttgaggatg aaatcaaagt aaaagcaatc aaaaactata
     1561 tggagtcgga cagaaaaaat cttagaaaat tgaatgagta tcgacgaatt ttaccaacag
     1621 acaaaacgat gcgttcttat attatggctt taaattaaag gagaagatac atgacaccta
     1681 cgcagttaaa agcaaaagtg aagaatattt ctagagaaaa agaagttgat ccgcagcttg
     1741 ttttaagaca ttttatgatg gaaaaattct tggaaaaaat ctcagagtct ccttatcgtg
     1801 aaaattttgt gttaaaagac ggcttcttga ttggctcaaa gtatggcatt gaaaatcgca
     1861 caaccaaaga tattgatacg actttacggg aaatgaaagt aaccaaagaa acattaacaa
     1921 cggttctaaa cgaaattttt tcaacgccga cgaaagaagg cattcaattt gagattcaag
     1981 gaatgaaaga aacgagagaa gccgattact atccaggatt tagcttacgt gtgctggcac
     2041 atttggaaaa catgcggcca gattttaagg tagatgtgac tacaggtgac tcgatctatc
     2101 ctgcaacgat cactcacagt cataaattga tgtttgagga tcgaacgatt cctttggaat
     2161 cctatcctac agaacaaatt attgctgaaa aattaagtgc aacctttgat tttttaacag
     2221 ataattcgag aggaaaagat ttttatgatc tctatactat tcctaaaatg gagaagatcg
     2281 atgagaagac cttgaaagat agtgtgagaa atacatttac acgtcgagga aaaactgatc
     2341 cgttaaagat atactatgaa aaggatatgg atgtactgaa aaatagtcac gagctaaaag
     2401 atacctggaa gaaatatcaa aaaagcaatc cttatgctgc gtcgatcacg tacgatgaga
     2461 caatggattc gattagtaat ttgatggaaa aagtgattca cgtcgaagaa aaagagcgaa
     2521 aactagctta tcaaagacac caacagatga atagagaaat ggagagataa agatgattaa
     2581 taatgtgaca ttagtaggac gcttaaccaa agatcctgat ttacgttata ccgcaagtgg
     2641 tactgcagta gcgactttta ccctagccgt caaccgaaat ttcacgaatc aaaacggcaa
     2701 ttgagaagcc gattttatta actgcgtgat ttggcgcaaa ccagcagaaa caatggcgac
     2761 attagctaaa aaaggtagct tgattggtgt agttgggcgg attcaaacgc ggacttacga
     2821 caaccagcaa ggacaacgtg tatatgtgac tgaagttgta gcagataact ttcaactatt
     2881 agaaagtaaa gcagcgacag agagccgtgc gcacgctgat caatcgtcca ccagtccaag
     2941 caccgccacc tttgaacaac ggaatacagc gacgcctaac aacaatggat tgaacgcttc
     3001 acagaatcca tttggtggac aatcaatcga tattagcgat gatgatctac cgttttagtt
     3061 tgagaaagaa ggagtaagca tggcaaaaaa aattagtctg acgatggatc tattgatacc
     3121 cgtaattgca tttatagacg ggatttcttt gggcgtcact gaaaatcctt tggtaaatag
     3181 aattgcgaaa ggatcactat ttcttgtatt cgtgtatctt gtctgttctt atcttttaaa
     3241 aagagcagga aaggataatt ccttttggcg attgactaaa gtcacagtag cagcgacagc
     3301 tgttatactt acggctattc taccgcttta tatgtagagt taacgggaaa tcgttgaact
     3361 tcttgcgaaa tattgctata atgaacatga aaaagaggag ttgcgctaac aacccctcat
     3421 taacagcacc gattaaagaa cggtggcaga atattagtta tttggaaata accgctgacc
     3481 tgctaaagta gacggttatt tctttttgtc atttttaagc atatttacaa tcaaactaat
     3541 caaagcaatg gtaaacatac caaaaccaag aatggtttga attgtctcat atgcggacaa
     3601 aaggcttact cctttcatag attttagcac ttacattcat aagcaccacc acctttccag
     3661 aagtcgccac cgtcttcaaa ctttactgtc ttgactatta tagcatatct aaaatcgaat
     3721 aaaaattctc aattttagct gtttttgcta taattttagc gaggtgatcg tgatggaaca
     3781 atatattggt ttactcgata aattagggtg atcaagacac agccattgct tgtgcgattt
     3841 acattacgca cgagtcatga accaatcaat tgtgtagtgg ccaatataga aatagcggat
     3901 aaattgctga ttttggaaga aggcaagtat actatttcag tgacaggtca cttcaataaa
     3961 cgaaagcaac tagtcattga atcaatgcaa attagaaacc ctgatcattt tacgcgatcg
     4021 atgagcatat aaaagaagag ctgcagtcct aaatgaggat tgtagctctt tttgttttac
     4081 atagaaacag taaaaatgaa aaggcctgtt taggaaggca caagcgcctt ctcaattgaa
     4141 agacctttct ctcactctaa ccgccaccgc cgttcgagaa gaaaggaagt gaacgctacg
     4201 cgttcactaa gataaagggt cttgcatagc aagacaaaat agaaaaaatt tcctctcacc
     4261 aaaaggttcg ctcctgttcg ttatctcttt attgatttgt cagaccggca acaagttgcc
     4321 ttttatcacc aaaaagcgac aaggacaagg gtaaatggca cgctgcgcgc ccttgtcttg
     4381 tcgcttttcg ttgattcatt ctggccaaca aatcaataaa gaaagaacta acgaaaggaa
     4441 gtaaaacctt tcctaagagg aggcagaatc aatcatgcga aagaaatttg attattgggg
     4501 tgttcccttc agcgaattat tcccaaacta tcatgcacca catactgtcg aatgcgattg
     4561 tggagaaaga gcgaaatgta tcaaaagcta ttgtttgtat caatgtccaa catgcgggaa
     4621 aaaatatacc ttgtcttatg gcgattatat tttaattgat gagaaaggaa agaaacgctg
     4681 atgaaaacac aacacaaatt attgactgta aaagaagtcg ttcaaatttt ggtggacaaa
     4741 gaagtcattt ctgaaaaagt agcagaagtt ttacttattc cgcggtactg gacgatagaa
     4801 acccctttag tagaggatgc accgactatg ctatttgaag acctagtgaa gattgcagga
     4861 tatgaactgc attatcaaga aaacttggaa acttatgaaa acgaatattg gttaaccaaa
     4921 ttagagaact aaaaaggaga aaaaaatatg gctcaaaatt actatgacga atttgtcaaa
     4981 ttacctttgg ataaaatggc acaaaaaatg gaggatatga cgttcctata caacgaaaca
     5041 cgtgttccaa aaaaacatta taaagaaaaa ttgtcggtag ctgtggaaga aatgattgaa
     5101 taaggtgtcg aaatgaatct tattgcgacg tactatcgaa cactggaaga actgaaaaag
     5161 caaaatgcaa aatggttttt ccaagcctta ctatgcttgg aagtaggtgt caaaccaagc
     5221 actattaaac cttctgaata ccaagcttta gaactaacgt atgcgaaatt tatcgaaacg
     5281 aaaaaagcca agactgtttc aagtgaatgg ttagattatt ttgaaaatat caataagtat
     5341 ggcgcatatt acaccatgaa aaaggaagac aacgaaaatg aataaggtga tttatcaact
     5401 aacaacggat tccatatcaa cgatttgaaa gaaaaaaagg tcaataatca tttataccga
     5461 aaagtgagaa tgtgtgaaac aaggacaaaa aaagcctagt aactaaggaa atggttctta
     5521 tttcgcacca atcgctccaa ccgcccccgc cgcttaggtg gtgcgaaata aacgggcaaa
     5581 gcccgataac ttaagagaaa gaaggtgatc catttgaaca agattattcc cttattgtta
     5641 gtactgtttg ttggcgaaat acttatgcga gatagtgcag ctgtacagtt aaacctcgct
     5701 gtatttttgg taatactggt gttttgtttg atcttttggc ggtttgtatt agtcctcgtg
     5761 agtctactat tcaagatgat tgcagccata tttttcatag ggttatttcg atgaaaggag
     5821 tgagtaccaa tgccaattgc aataggaaat aaacggttac ccgtgacatt agatgaaaag
     5881 agacaaaaag aattgcagca actaaagcag aagtatggca aaagtgaatc taagattatg
     5941 tgtattgcgt tagatctatt gatcgcgcaa gaaaaagcag gatttgatgt accagcgcta
     6001 aaaaagtgac gtcacttttt aatttgatct atataagtga tatcactttt accaaggagg
     6061 gaattaaatg ttaaaaacta aaaagaattc gtacaaatta aagcttagta tactatctat
     6121 tatttgtctt ttaggcatga taattagtgg tcatttgtat agagtggcat atacgcagtt
     6181 tattgagtac aaggttcact ctttaggatt agctggggat cgatacgttg aatatgtcca
     6241 ttattcatta aaatgttctt catatattct tctttggctt attttttcat tgtttgaatt
     6301 gctaaattta atagcaatat cttcatatca cttagataag ctaaatacca aatctagccg
     6361 ttataacgaa gtgtttaagg aaaatgtgaa gttagaaagt gacttaaaca agctaaaaaa
     6421 ataaaaagaa gaaaacataa gttaactaaa attattttat taagaatgat ggaatataag
     6481 gataaggtta ccaacataac gatcattact tctctgatat gctatggact tctatcaatc
     6541 agtattttgt ctatagatta tggagtaact atactgcaag ccttagtttt ggctatgcct
     6601 atgtatctat ctttgtttat catgcatgaa atatataagc tatttgtttt atcaaaaaat
     6661 gtagaaatac gggtaaatat tttggtgaat agttatcaaa aagaactcaa agaaagaaga
     6721 cggtggcggt atttattgaa aggatgaaaa agtaggattt gacatactgg tattcagaaa
     6781 gtgccgtcac ttttaaaaca atggaaaagg tggagataag atgaagaaaa aacaaactaa
     6841 actatcatta gcagatattt aacacagtta accgcaactg aagatggtgt tgttgaattt
     6901 gaacaatctg aaattgcggt agtagatgat cgttacctta aaatgcctta cttctttgac
     6961 caagcaaagg tcatttgcct ttgtcactac gatggggtac gtgattttag gcgttaaaaa
     7021 gtagtgatta tatcaacttt acgagtaaat ataaatttag acatgaaact gaattatcca
     7081 atggaaaatc atggtttata atctagaaaa attatagtaa tgattggatt atatatattc
     7141 tttcgtattt tcgtacaact gtagcttgtc aaaattgaga aatttaaccg attaggtact
     7201 ttacatcgtt atttgggtaa tttctaaaac atgaagctta ccaacgaata aactgagact
     7261 atcttattaa ttgatcaata taagaaaata tctttgagga gatgatgtac tatgttttcg
     7321 tctatccacg attggttagg tggtcttttc ggcggtggcg gtaaaggttt ttaa
//
LOCUS       DAMYXM010000099         6991 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.103, whole genome shotgun sequence.
ACCESSION   DAMYXM010000099 DAMYXM010000000
VERSION     DAMYXM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.103"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(156..614)
                     /locus_tag="PNS21_002565"
     CDS             complement(156..614)
                     /locus_tag="PNS21_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDI5160221.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(627..1958)
                     /locus_tag="PNS21_002566"
     CDS             complement(627..1958)
                     /locus_tag="PNS21_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HDI5160222.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1969..3411)
                     /locus_tag="PNS21_002567"
     CDS             complement(1969..3411)
                     /locus_tag="PNS21_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDI5160223.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3398..4162)
                     /locus_tag="PNS21_002568"
     CDS             complement(3398..4162)
                     /locus_tag="PNS21_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HDI5160224.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4266..5141)
                     /locus_tag="PNS21_002569"
     CDS             complement(4266..5141)
                     /locus_tag="PNS21_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDI5160225.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5396..5480)
                     /locus_tag="PNS21_002570"
     tRNA            complement(5396..5480)
                     /locus_tag="PNS21_002570"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5444..5446),aa:Leu,seq:caa)
     gene            complement(5498..5569)
                     /locus_tag="PNS21_002571"
     tRNA            complement(5498..5569)
                     /locus_tag="PNS21_002571"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5535..5537),aa:Gln,seq:ttg)
     gene            complement(5579..5651)
                     /locus_tag="PNS21_002572"
     tRNA            complement(5579..5651)
                     /locus_tag="PNS21_002572"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5616..5618),aa:His,seq:gtg)
     gene            complement(5713..5797)
                     /locus_tag="PNS21_002573"
     tRNA            complement(5713..5797)
                     /locus_tag="PNS21_002573"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5761..5763),aa:Leu,seq:caa)
     gene            complement(5818..5888)
                     /locus_tag="PNS21_002574"
     tRNA            complement(5818..5888)
                     /locus_tag="PNS21_002574"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5854..5856),aa:Cys,seq:gca)
     gene            complement(5934..6005)
                     /locus_tag="PNS21_002575"
     tRNA            complement(5934..6005)
                     /locus_tag="PNS21_002575"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5971..5973),aa:Gln,seq:ttg)
     gene            complement(6017..6089)
                     /locus_tag="PNS21_002576"
     tRNA            complement(6017..6089)
                     /locus_tag="PNS21_002576"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6054..6056),aa:His,seq:gtg)
     gene            complement(6092..6165)
                     /locus_tag="PNS21_002577"
     tRNA            complement(6092..6165)
                     /locus_tag="PNS21_002577"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6131..6133),aa:Trp,seq:cca)
     gene            complement(6172..6252)
                     /locus_tag="PNS21_002578"
     tRNA            complement(6172..6252)
                     /locus_tag="PNS21_002578"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6216..6218),aa:Tyr,seq:gta)
     gene            complement(6254..6329)
                     /locus_tag="PNS21_002579"
     tRNA            complement(6254..6329)
                     /locus_tag="PNS21_002579"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6294..6296),aa:Phe,seq:gaa)
     gene            complement(6365..6437)
                     /locus_tag="PNS21_002580"
     tRNA            complement(6365..6437)
                     /locus_tag="PNS21_002580"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6402..6404),aa:Asp,seq:gtc)
     gene            complement(6447..6519)
                     /locus_tag="PNS21_002581"
     tRNA            complement(6447..6519)
                     /locus_tag="PNS21_002581"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6484..6486),aa:Val,seq:tac)
     gene            complement(6535..6606)
                     /locus_tag="PNS21_002582"
     tRNA            complement(6535..6606)
                     /locus_tag="PNS21_002582"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6571..6573),aa:Glu,seq:ttc)
     gene            complement(6621..6710)
                     /locus_tag="PNS21_002583"
     tRNA            complement(6621..6710)
                     /locus_tag="PNS21_002583"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6674..6676),aa:Ser,seq:gga)
     gene            complement(6750..6823)
                     /locus_tag="PNS21_002584"
     tRNA            complement(6750..6823)
                     /locus_tag="PNS21_002584"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6787..6789),aa:Asn,seq:gtt)
     gene            complement(6833..6948)
                     /gene="rrf"
                     /locus_tag="PNS21_002585"
     rRNA            complement(6833..6948)
                     /gene="rrf"
                     /locus_tag="PNS21_002585"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2108 a         1546 c         1144 g         2193 t
ORIGIN      
        1 tgaaaataag ttttctaaat cataggagca acaaaaaaat tcgataatta acaaaaatca
       61 aaatgattaa atctagtgat tgaaacatat catttcaaaa aaattttgta aattcaccca
      121 taaatatcac cctatttaac ttttttcatc gtacactaaa attcaaaaca actatcttta
      181 atctttactt cttggttttg ttttaataaa tttccttcaa aaatgcctgg agaaactaca
      241 atgatcaccg tatctttttg tgcttgctcc aacttcttca aatctataat agctaacagt
      301 gtcccttctt ttacctcctg gttactttca acccgtatct gaaacggact tccttcgagt
      361 tcaactgtat ctaaacccat atggatcagt atttttctgc cagattcact ttcgatcgtt
      421 atggcatgtc gggtaggaaa tatattatcg attattcctt taactggcgc atggatatat
      481 ccatcatgat tcgttatagc gtatccaact cctatcattt ttgaagaaaa gacctcgtct
      541 tttactgctg ttaatggaat aatttgacca ctagctacag cttttagaat gtttcttgtt
      601 ttaaagaaat gcacttttta tcacctctac tttaatgctg gccaatattt tttgtttact
      661 ttgatcattt catctagtat tttttttgcg actctggcat ttggtacggt ttgattcaat
      721 gtaaatgctt gtaaagcttt ttgataagaa ccttcaaagt aagcatctac tagtaacttc
      781 tctactgcaa cttgtgcctc catcaatcct ttatgaaaat cactgattgg ttttcttagt
      841 gtaattggct ccacaccagt tgcaccaaca tatgccggta tttcaacaac tgcatctgac
      901 cgtagattcg gaatcgcccc ttgattagga acgattagca taaagcgacg acgatcatca
      961 ttcaacaagg aaatagctat atcgacgata tattgtccat gctccccaaa attaaagttc
     1021 aatacttctt ccacaatacc gcggcgtatt ttatcagcca tttctttggt atttttctct
     1081 cgtccatcca tgaccatatt agcacgtgta tactctttgt cagtatgctc aacgaccata
     1141 tcaggatata agtaatactc taagtaatta ttcgggatat tacttggaaa atttttcgtg
     1201 atgactgaca tcatttgaaa ggttttttgc caagttttat ctcctatatt aaaatcggcg
     1261 acttgcattt cttttgttat tagtttttca ataatctctg gcataatttc tcgttgcaat
     1321 tctttatcat aaatcgatgt ataccagcca aagtggttta aaccataata cgtaacgatc
     1381 caattcttac gatcataacc gtaattcact gcaatcgttt cttcaattga aatagtcata
     1441 tcacaggcat taatcatttt tacattcgga tatttacgac gaacagcttc agaaacgatc
     1501 gactctggat ttgtataatt caaaatccat gctttggggg catagtcttg tatatgatcg
     1561 acaatttcca ataaaccttt gattgagcgc atcccataag caaatcctcc gagtccacac
     1621 gtttcttgtc ctactaatcc atgtttaagt ggaatctttt cgtccttttc tcgcatctcc
     1681 aatcctccaa cacgaatttg tgagaaaaca aaatcgcatc ctgtaaaagc aagctttgga
     1741 tcatccgttg cacgaatgcg aatatttgaa aaaccttttc ttttcagcat aaatttaaca
     1801 atcaagtaca tatccatatt tctttcttca tcaatatcat ataaacgtat ttcagataat
     1861 ggcaaccgtt tttcattgtt caaaatggct tgaacgattc ctggtgtata cgtactcccg
     1921 cctccagcta tagtgataat ctgttttttc atcatcatct tttcctttct attcttctaa
     1981 taatttattg aattctgcca taacataagt cacttctaag ccgatgataa tttgataatt
     2041 tttttcagtt ggtttaatga tcccgcttat ccctaattgc ttgattcgct gttcctcgac
     2101 catggtggga tccactacct ctacccgtag gcgtgtatta caactataga catccaggat
     2161 attggctttt cctccaaacg cttgtagcat ttttgttgcc atatattcat aatttttact
     2221 tgctaaccct aactctgttt cttctaaggt tacttcattg ccaaactctg ccttagcttc
     2281 acgtcctaat gtctgaatgt ttctcttatt gatcaaataa taaaaagtaa cgtaatacaa
     2341 ggcaaaaaag aaaattccga ttggaagaat caaaatagca ttggtagcga ttcgaaaatt
     2401 gatgagataa tcaacaatac atgctccgaa tgtaaaacca attcgtatat tgaataagta
     2461 acagacaatc cctgctaaac cggcaaaaag tgcatggatc acgtacaaca taggagaaag
     2521 aaacatgaaa ctaaattcaa caggctcagt gatatttgca ataaatgaag ttcctgcacc
     2581 gcttcccatg attcctttga ccatctcttt attttcttta taagctgctt tatagatagc
     2641 tagacatata gctggtatac caaacatcat tactacaaaa aaaactgata aaaacagtcc
     2701 agcctgagga tctccgttga taaagcgtgt catctctcct ctgaaaactt ccttgctagg
     2761 agatgtatag cttcctaaat cgtaataaat atacgtattc aacacatggt gcagtccaaa
     2821 ggggatcagc aaacgattca aaaacataaa tatccccaca cctaatgctc ccatatcaac
     2881 gagcagtttt gcaaaattat caatcccaat ttggatgagc ggccagataa agcccaaaaa
     2941 aacagctgcg attgtttgta gaatcatcac catagtcaat gggaattttt cgcccgcaaa
     3001 ataagaaaaa actactggta atttttgttc tttaaagcga ttatacgtcc atgccgctac
     3061 taaaccggaa ataattccac caaaaatccc catattcact tctgggtcca taatttctaa
     3121 gccttttttc aaggtagcaa tcgctaaaaa accagtaagt gcaggtattc ctttgtcctt
     3181 atttttgaca aaaccgattg caactcctat agcaaataag atatccaagt ttccaaaaac
     3241 ggcatttcca gctgcaccaa ttactgggat gttcagcata tcgtcagctg ataaacgcat
     3301 gatcaatccc gctaatggca tcgctgcaac tggaagtagc atcgctcttc caagcttctg
     3361 cacatttgat ttaacattca tttttattcc tcctctatta aatatcctca tatgttacta
     3421 actcaatcaa aggatcattt ttcacttttt gagcaaatgc tgaatgaatt aaaaattcga
     3481 cttggtgggt acgaggatac acaaaacttg aaccatttaa caattcctga tccaaatacg
     3541 ctgcgtgaca catgacttcg atcgttttat cttctctcaa tatcatgagt aaacttgcca
     3601 aatattcttc cacactcgta ttgatttgtc gttttttttc atcagtttct cctacgacat
     3661 cgaaacaagg ctcaaaataa tcgacatgac gaacttcatc ttttcgttca aagttgctcc
     3721 gaaccggcaa atcatatttt tttgctaatg taacaactac ctcctgattt tcatccaaag
     3781 tatgagtatg atgatgactg tctaaatgag ttggtatgat ccccgcatga taaatttttt
     3841 gaatctgagc attccattcc tgatacagtt gatctttgtc aatgttaaat ggatgctgat
     3901 aataagataa ggatctgaac tctccgcttt cgataagagt atcgacagtt ttgaggagtg
     3961 gtttgccaca cgtcaatgtt aaatggactc ctacacctag cctaggcatt tcctttctta
     4021 gtttcacagc atgctcgaaa cccggcatat ttgccatcaa tgtagttgat gtcaaaattc
     4081 ctcgctgata agcatccata atcccataat tcacaccatg gctatagcca aaatcatccg
     4141 aattaaaaat cacttttccc atttcattct ccttcttttt tctactaaaa taggcgcaac
     4201 caaataatca tagtccattt actatgtgtc atttggttgc gcctatagat agttacgccc
     4261 ttattttaat agattcttgc tagatgaatt gccaaataga tgatttcatt ttcttgaatg
     4321 gcaagttgaa attgctgttc gatgatatct ttaatcttta gtgctgtaca atacgctttg
     4381 ctatgtttaa atgtttcagt aaaaaaacca atattttcta ctggcgtttc ttcctgataa
     4441 atcaagcgtt taaccaggaa tttgatatga accattaagc gatgataagt cagtgattct
     4501 ttatcataaa cgtttgaata ggatttttct agatgggaca aaatcgtggt cgtcatttct
     4561 actattttta agctctttaa actattaggc tctcctagct cagcattaat gatatgcata
     4621 gtgatgaatc ctacttcatt tgaccctaac tcggtttgat atcgatgatt gattcgctcg
     4681 accacttcta ctgctatatc atattcattt ggataaagga atctcatttc tggcaacata
     4741 attgctgtga catctctatc ttctcttttt cgttgaatag caaaataaat atgatctgtc
     4801 aatgtaatat agatatttga atctaatgga gaggagatcc tagctgttat cagttcaatc
     4861 acttcttccg caaaatccat aaattcgatt ggaatctgcg agactatctc actgaatcgt
     4921 ttgatctttt ctttagaatc taatacaaat aatttgtcag catcctcaac atcaaatggc
     4981 ttactcactt tagcagaaaa gccaatccct ttccccatcg caattttttc tattccatcg
     5041 tcatcgatca atacagcatt atgattcagc tttttcatca catacaccgt taattcacct
     5101 cctctatatg ataaaacaaa acaacttgca gactccttca tacatatttt tgtctccaaa
     5161 caatttttaa agacaaaaaa cgacctaaga aagaaccctt tcttaggtcg tgcctaattg
     5221 aatagtagca ccctacataa tttacaaaaa tagtttaaca cataataaaa gcgttatcaa
     5281 tatctttttt actcgtttta tgataatact tgtatcgaat tgatttggag gtaattacat
     5341 aaaaacaaaa aaaatatagc aactctctat aagaaagttg ctatattgat aattataccg
     5401 gcggccgggg tcgaaccggc acgtcctcaa cggacactgg attttgagtc cagcgcgtct
     5461 gccaattccg ccacgccggc aaatttaaaa ataataactg gggtagctgg attcgaacca
     5521 acgcatgagg gagtcaaagt cccttgcctt accgcttggc tataccccaa gaataaaggg
     5581 cgaatgatgg gaatcgaacc cacgaatgcc ggagccacaa tccggtgcgt taaccacttc
     5641 gccacattcg ccatcataaa agatgttgtt taatttctca ataaaaaact aagatgaaga
     5701 catcttagtc aataccggcg gccggggtcg aaccggcacg ccctcaacgg gcactggatt
     5761 ttgagtccag cgcgtctgcc aattccgcca cgccggcaag ttgatttatt agtactaagg
     5821 cggtaaccgg atttgaaccg gtgatgaagg ttttgcagac ctctgcctta ccacttggct
     5881 ataccgccat tatactatta tcaatctata aaataaagaa taaattatag aaactggggt
     5941 agctggattc gaaccaacgc atgagggagt caaagtccct tgccttaccg cttggctata
     6001 ccccaagaat aaaaagggcg aatgatggga atcgaaccca cgaatgccgg agccacaatc
     6061 cggtgcgtta accacttcgc cacattcgcc atggcagggg cagcaggaat tgaacccaca
     6121 ctaacggttt tggagaccgc tgttctacct ttaaactatg cccctataaa atggaggaga
     6181 gtggattcga accactgaac cctaaggaac ggatttacag tccgtcgcgt ttagccactt
     6241 cgctactcct ccatggtggc gcgggacaga atcgaactgc cgacacacgg agcttcaatc
     6301 cgttgctcta ccaactgagc taccgcgcca tattttaaaa ttattaattg aataacaaga
     6361 acgacggttc cgacgggaat cgaacccgcg atctcctgcg tgacaggcag gcatgttaac
     6421 ccctacacca cggaaccagt tcattatgga ggttaacggg atcgaaccgc tgaccctctg
     6481 cttgtaaggc agatgctctc ccagctgagc taaacctcca acattcaatt aaaatgaccc
     6541 gtacgggatt cgaacccgtg ttaccgccgt gaaagggcgg tgtcttaacc gcttgaccaa
     6601 cgggccaggt aaatttgcta cggagagtaa gggattcgaa cccttgagac agtttggcac
     6661 catctacatg atttccaatc atgctccttc ggcctctcgg acaactctcc agagtgaaag
     6721 aagctcaata ctctttctca aaatgagaac tccggcagta ggactcgaac ctacgacatc
     6781 atgattaaca gtcatgcgct actaccaact gagctatgcc ggaataacat cagcgtggcg
     6841 acgtcctact ctcacaaggg gcaacccctc actacaatcg gcgctaagaa gcttaacttc
     6901 tgtgttcggc atggttacag gtgtatcctt ctcgctatcg ccaccacact gtggtgttat
     6961 cttttattga gtaaattttg ttcactcaaa a
//
LOCUS       DAMYXM010000100         6826 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.104, whole genome shotgun sequence.
ACCESSION   DAMYXM010000100 DAMYXM010000000
VERSION     DAMYXM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.104"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            60..950
                     /locus_tag="PNS21_002586"
     CDS             60..950
                     /locus_tag="PNS21_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HDI5160226.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(1023..1847)
                     /gene="nadE"
                     /locus_tag="PNS21_002587"
     CDS             complement(1023..1847)
                     /gene="nadE"
                     /locus_tag="PNS21_002587"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDI5160227.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1859..3331)
                     /locus_tag="PNS21_002588"
     CDS             complement(1859..3331)
                     /locus_tag="PNS21_002588"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDI5160228.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3968..4330)
                     /locus_tag="PNS21_002589"
     CDS             complement(3968..4330)
                     /locus_tag="PNS21_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDI5160229.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4553..5305
                     /locus_tag="PNS21_002590"
     CDS             4553..5305
                     /locus_tag="PNS21_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDI5160230.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5445..6368)
                     /locus_tag="PNS21_002591"
     CDS             complement(5445..6368)
                     /locus_tag="PNS21_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI5160231.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6383..6655)
                     /locus_tag="PNS21_002592"
     CDS             complement(6383..6655)
                     /locus_tag="PNS21_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160232.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2092 a         1354 c         1162 g         2218 t
ORIGIN      
        1 ataactttta ttaacaaata tgttaatata taagaaaaac cttttaagag gtgatatata
       61 tgcaactaca aataaacgaa gaatccttac cggtatatga agcattagct agtaaaacaa
      121 ggatcaagat catccagcta ctttccaaaa aaaagatgaa tgtcaaagat ttggcaaaag
      181 aacttggagt gagcagtgct atcacgacga tgcacgtgaa aaagctagag gaagcaaaca
      241 tcatcaaaac agaaaaagta ggacaacaaa aaatttcgag ccttcgtgtc gataaaatcg
      301 atatcagttt tccagaaaaa atattcaatg cttttgatac gaaagaaaca tcgataccta
      361 ttggtcacta tacaaattat gcaattgaac caacatgcgg tttagctacg atccatgatt
      421 ttatcgggaa agtggatgaa ccacgctatt ttatggatcc tcgaaggatg gatgcccgca
      481 ttctctggtt taccagcgga tttgtcgagt atcaggcacc taatttttta aatacagagg
      541 atacattgga gatgttggaa gtttctgtgg aaatttcttc tgaatttcct ttttcaaatg
      601 acaattggcc ttcagatatc actttcagcc taaatggtgt ggagctagga acatggacga
      661 gccctggaga ttttgctgat atccgcggta aatatacgcc tgattggtat ccagataatc
      721 ttaatcaata cggcttgctg aagacgatcc gaattaccaa acacttgacg aatatgaatg
      781 gagaaccctt gtcaaacatc acgatcaacg acatccctaa agagcaagac acttggcatt
      841 tgcgaatcga agtgaaagac gatgccaaac atgtgggggg ctgtacttta tttggtaaag
      901 gattcggtaa ttacgatcag gatatcaaat taaaagttta ctacagctga tatttttctt
      961 ttctatttta taaaacaaaa acagccaatc cctatgaagg gacggctgtt tttgctttat
     1021 tgttatttcc agaaatcatc caaaacggaa attggcaaat gtcgtttatg ttgtgtcttg
     1081 ttccaccatg cttcgatttt ttcttgtgct tgagcgggaa cttcttttcc ttctagataa
     1141 tcatcaattt cattatatgt gacacctagt gcgacttcat ctgcaatcat tggtttccca
     1201 tcttcaagat ctgcagtcgg aatttttgtg tataattttt ccggtgcatt caatgcttga
     1261 agcaattgtt tcccttgtcg tttgtccaaa cggaataatg gcaaaatgtc ggctccacca
     1321 tcaccaaatt tcgtgaagaa tccagtaatg ttttctgcag catgatctgt ccctaatacg
     1381 gctcctgctc tttctccagc tacggcatat tgcgtaatca tacgttgacg tgctttgata
     1441 tttcctttgt taaaatcgct gattcccaca cctgcttcgc ttaatacttg tacttgtgca
     1501 tcaacagctg gtttgatatt tactcgcaga ctaacatcgg gctggataaa gtcaagagca
     1561 gcttttgcat cttcttcatc tgcttgttca ccataaggaa gacgaacagc gataaattga
     1621 tagttttcat cttttgtttc ttcacgcatt tcttccatcg ttagttgcgc caaacggcct
     1681 gctaaagtag aatcttggcc gccgctgata cctagtacaa aagttttaag aaacggatat
     1741 tttttcaaat atgctttcat aaagtcaata cttttacgta tttcttcttt tggatcaatt
     1801 gttggtttga cacctaattc attgataatt tctgtctgca ggctcattta ttctcctcct
     1861 atgcttctgt atgaagttct gctacgcttt ttttcattcg ttccatgata gccattttat
     1921 ggttccagca ttcagtagac agatcgactg ggtatttttg cggattcaag tctcgtttat
     1981 actcttccca tagtgaatct agattttctt ttgtatattc tttgatttcg tttagtgcag
     2041 gtaattcgta aactcttttt ccttcgataa agatatcttg taaaactggg cgagctgtaa
     2101 agtctcgaac agttttattg ataaacgtat gcactggatg gaacatgaag atagcttctt
     2161 cttctcgagg gtcttcatcc cataatgtca cgtagtctcc ttcagatttc ccatcaaaat
     2221 ttcgagtgat gcgccacact tgttttttcc ctggagtagt tactttttct gcattgcttg
     2281 ataacttgat cgtatccatc atttttcctt catcatcttc gatcgatact agcttgaata
     2341 ctgcaccaag ggctggctga tcataggctg tgatcaattt tgtcccaaca ccccatacat
     2401 caattttagc tttttgcatc tttaaattca aaatcgttgc ttcatccaag tcattcgaag
     2461 catagatttt tgcttccgta aatccggctg catctaattg ttcccgaaca cgcttagata
     2521 tataggccat atctccactg tcgatgcgta cgccttggaa gttgattttg tcacctaatt
     2581 ctttcgccac gcgaatcgca cttggcacac ctgatttcaa cgtatcataa gtgtcgacta
     2641 agaacacgca gtctttatgg gttttagcgt aggccataaa tgcttcataa tcatttccat
     2701 aagattggac taacgaatga gcatgggtcc cgctagctgg tatcccaaat atcttcccag
     2761 cacgtacatt gcttgttgca tctgctccgc cgatatatgc cgctctagtt ccccagactg
     2821 ctgcatccag ttcttgtgcc cgtctagttc caaattccaa tagtggatct tctccgatga
     2881 cagatttgat acgagctgct tttgtcgcga tcaacgtttg gaaattgacc atattcaaca
     2941 gtgctgtttc tactaattgg cagtgcgcta aaggcccttc cacttggatt aatggttcat
     3001 ttgcaaaaac tagttctcct tcacgagcag aacgaactgt tgctttaaat tcaaaatttt
     3061 gtaaatactc caaaaaacct tcaggataga cttctaatcc acgcaagtaa gcaatatcgg
     3121 aatcattgaa cgtcagattc tctaaataat tgactaaccg ttccaatcct gcaaaaaccg
     3181 cgtatccgtg gttaaaaggc atttctcgaa agtaacattc aaaaacagca tgtaaatcag
     3241 ctcgacctaa ttcccagtat gtctgcatca tattgatttg atatagatcc gtatgcagcg
     3301 tcaaactgtc gtctgggtaa gtattattca tcttgtatta ctcctcttgg caagttttca
     3361 tttcctaccc attataacaa aaaaaattaa taggaaaaga aatcttccat tttttctgaa
     3421 aaagcacatg ttttacggat atttcttgct aaaaatgaac tccatctgct aaaactatcc
     3481 tttcttattg gtatttaacc taaacgagaa agaatactta ttttattcaa tgtatccgag
     3541 acacgaagaa attagcgttt tctatttttc ttacttgata aaataagtag attttcctac
     3601 tacaatcgta gccgttaaga taccgatgat tgaacaaata taaccaaatg tctctacaac
     3661 ataatcaatg gcaaaagttt ttgaagcaat aatacgtctt tccttttttg cttcttatcc
     3721 ccaaagaaac ggatataact tattatgtat tgaatttttt tggaaaggcg aattatgaca
     3781 agatatacct tgcgttgaaa aaactcgcgg aaaaaatatc acacgttttt aaaaagatac
     3841 aaagtaagaa agcagctgtc aatagaatgg aaacaaaaat ggcgagacat caatcagtct
     3901 cgccttgttt tttattgtat acattagata tggagcaggt taccataaag atgattatta
     3961 gtcgttatta aatagaatcc ttcgtatttt tttgttctat cactacaata tctttatttg
     4021 gacgtttgaa ttgtattccg gcacgttcta gctcctgttt cactcctaca tggactttga
     4081 catcttctcg tgtcattttt aaaacgtgat gaatcgcata gtcgatttct tgccttgtga
     4141 tttttttctt cgtattgatc atcacgactt gctttttatc atatgtgtct accaatagca
     4201 gttgtgttcg atctaacgaa aataatttgt aataattaat agccgtattc ccaaaaaaga
     4261 attccactac tttaggataa aatgtcttaa tatttaaatt actattcaca ggtgtttcga
     4321 ttagcttcat ctcattcctc ttttctatat agtcgattta gatccaatct taagttatgt
     4381 taccatcttt cctagcatta aggaatcggt tacaattgaa cctttaaaga caaaaggatt
     4441 taccttttat cacttaatag tataacataa tttcgtgaga aaaaaataca attttttctt
     4501 gaccatctcg ttacgtaaag gattatgctt acatttgtca ggaggaaaaa taatggagta
     4561 cacaataaag aaaatgtcag agatatcagg agtcagtccg agaaccttgc gcttttatga
     4621 tgagattgga ctgctaaaac cagctaggat caattcttca ggttatcgga tttatggcaa
     4681 aaaagaagtc gatcgtctgc aacacatctt attttatcga acaatggcat tcaagttgga
     4741 tgatatccaa gaggtcttag acaatccatc ttttgaccac cagaaagctt taatcaagca
     4801 tcagcaaatg ttgctggaaa aacgggcaca aatcgatacc ctcttgacaa ctgtccaaca
     4861 gacacttgat atgtacgaag gaggaagaaa aatgagtgac aaagaaaaat ttgaagggtt
     4921 taaacaacaa aagttgaaag aaaatgaaga atcctatgga caagagattc gacaaaaata
     4981 cggaaaaaaa aaggttacag catccaatga aaaatggatg aatatggata aagaaacgta
     5041 tgaagaaatg caaactgttg aaaagcagat gttatttgat ctcactcttt acttaaaaga
     5101 accagaagat gaaagcttag cagaccgtat cttccaagct cataagaagt ggttctctta
     5161 cagctggcct gaatatcagc caaaagcgca taaaggacta ggaatgatgt acgaagccga
     5221 tgaacggttc actgcatatt atgacgagcg aagtggtcat ggtgcagcaa aagctttaaa
     5281 tgaaattatc caaaaaaagg cgtaatcttt tacatgatcc aacaaaaaag tacacgtaaa
     5341 atgacctaat aacaaaacag cagacaccca agttagattt ttatgctaac ttttggtgtc
     5401 tgctccattc tttcacaagt tttttcatcc tactttaaat atattcatcg ttgaataaac
     5461 gtataaggta ttttcacttt atcctttttt tctgtaagaa acaattgaaa tgccatttct
     5521 ccgcaagctt ccagatggtg gtcgatggtt gggaaaccaa gaagctcgct gatcagcaaa
     5581 ttatcgcgtc caatcaccac tggtgtctta tcttgcagat agctttgcaa gatgcctgct
     5641 gcaaccatat ccgaatttgt aaagacacca tcaactccaa gctctttaaa aaagaagcct
     5701 gcttgttttc ctttctctgc atctatacaa tcccaaaaaa caagtgattc atcaaaagat
     5761 ggaaaaagtt gatgacatag ctgcaaagtg atttttgagt tataacttag cctgccactt
     5821 cgccccaacg tgatgcccaa ttgctttata ccttgctgct tcaaataagt aagaccttct
     5881 gtaattgatt gttctcgatc aataaagaca cagcttgcct gttttccctt gatttcttca
     5941 caaaaaacta gaggtccata tttttgatat gccaataaat catctagtgt attggcacga
     6001 gacgtaatga tcaatccatc gaaagcttta gctgcaaatt cttcaagata ccttcgctct
     6061 ttttctttat gatagcccgt cggtaaaagt gtcactttgt aatcataatc aaacgctgct
     6121 tcaatgatcc cactgatcaa ctgatcaaaa tacggatgat tggtataagg cagaatcact
     6181 ccaatgcttt tcgtttttcc ataacttaac tgtctggcat ttgcattagg gatataatcg
     6241 acttctttca tgaccgcaag tatttctttc cttttttcct catctacata tggatgtcca
     6301 ttgattacac gtgaaatagt agaaactgaa taacctgtga gctttgcgat atctcgaatg
     6361 tttgccactg ctgcttcgcc acctatacaa aaagattttc ttgtaaatag tctaccacag
     6421 ctggaccgca ttcaaaaaac tgatacattt ttccttcatg cgaaaaactg atccgctgcc
     6481 cgccccaata tatatctttt gtcaaatttt cgatttcatt cattggtatt tcttttacta
     6541 tattcctatt caacccttga aaactgtaca acgttaatac ggatcccttg aactcgacaa
     6601 acgcataaaa ataattgttc gttgtgccat ctgttatctc tacataagta ttcattgcaa
     6661 gccctccttt tgatactttg agtatagtgt atgaaacgcg tctcatagca aggagtttgt
     6721 tttgccaaag aaaagtttta ttcctctttc attattacca aagtctctta ttttaaattg
     6781 aatacattat aatgatactg taaatatatc tttattatta gtgaat
//
LOCUS       DAMYXM010000101         6798 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.105, whole genome shotgun sequence.
ACCESSION   DAMYXM010000101 DAMYXM010000000
VERSION     DAMYXM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.105"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(718..3867)
                     /locus_tag="PNS21_002593"
     CDS             complement(718..3867)
                     /locus_tag="PNS21_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDI5160233.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3880..5079)
                     /locus_tag="PNS21_002594"
     CDS             complement(3880..5079)
                     /locus_tag="PNS21_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDI5160234.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(5069..6664)
                     /locus_tag="PNS21_002595"
     CDS             complement(5069..6664)
                     /locus_tag="PNS21_002595"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDI5160235.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
BASE COUNT         1908 a         1400 c         1105 g         2385 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttgggca cttgaatctc ggctattttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcgtccttc acgaatgagc tgattgggaa actcttttgt
     2041 taaccaatca ggctctttcg tcatctcttt gatggcccga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga ttgcagattt cgttggcctg ccatttcgaa aatcaaattt
     2161 tgtataggca ttatctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gctgggtcca aataactttc ttttttgaca ggttccattt gatcaatcat
     2281 acgattgatc tgttctgatg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctagtgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 gacagagcca tcttctagcg cattcttaat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtt gtacgtgcca attgcccttt ggcttgtttc cgattctcag gaaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt ggcccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttc tttgcatatt tcaacgccgc
     2701 atctagtttt tgacgggttg taatgattac aacatttgca ttggcatctg ccaacagggt
     2761 ttcacttaat tctttggcac ttcccgttcc aacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaattttgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgttcgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ctttcctgaa ccagtcgcat gccacacata
     3001 ccctgattga tgtttatttg cggcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgagggttt tattatcttg atcttcactg acgattgtat aatccgcaat
     3121 caaacgatga gcatccggga tatttaacac ttgtttacaa aactcatata agttttctac
     3181 tttttcattg tctttcgttc gccaactaaa taaaaactta gggtgcaagt ctttcggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaatgcctg
     3361 ataaacacca tcttttgcag ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gattaataag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aagtagagat tcctccacca atgtcttgat tggaataaag
     3541 gataagtgag acagaaccca attgcgaatc ttcccgttca atcgtgatcc gagccatccc
     3601 attttcacct tttaaccatt tcgcagcatc aaatggggtt ttcgttcgca gcagtaattc
     3661 cgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagata attttttggc gtaaattgac ccaaaggtct tcttctgatt ttagatccgg
     3781 acgatacgtc cactgattat gcccttctcc aagtacacga atcaactgtt cttcgacttc
     3841 tgcttcctca cggtgactaa ttttcattct tcttccctcc tagacaaaca tcttttgtaa
     3901 gaagcttttt ttcgtttctt ttagtaaatc taacttacgt tgatgaagag cgatcgtatc
     3961 atctagctgt ttgaagaaag taccgatttt ttgttgttca tcctttacag gaaccttaac
     4021 actgatattc tcaatatcag aactgttata agaaggcaat gcccctactt gggagtatct
     4081 tggcaaattt atagtttcaa acaatatttt accaaaatag atatcccaag tattattaaa
     4141 taaataagcc atggtattgt tatctattaa gaaagggctt gtgaccaaac gttttctatt
     4201 aagcattatt gctgcgccaa ctttagcaaa tataatcgct ggcacatctt ttattggctt
     4261 ccatttttta cgctcaattt gctcattaga tacataatga tttgcatttc tcatttcata
     4321 ctcattacca atgttattca tgtcggaaac tttaaaaaat ggagtacctt cagaaccacc
     4381 ttgctcggca tcaggaaatc caattcccga ttgagtttta cctaattctc ccaacttacg
     4441 ctcttcccaa tcttccgtaa aaccaggaaa ccggatttct ggcacttttg ctccgttttc
     4501 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta acttacgttg
     4561 atgaagagcg atagtgtcgt cgagtttttt gaagaatatg ccgatttttt gttgttcttt
     4621 taaatcattg ggaatataga atactgtatc tgaaactttt ttccagtctg aacgaggcat
     4681 ttttgttcca gatgtatcat ttgcagcctt ttgatatcta tcagcttgaa taagagaata
     4741 aataaagtcc gagtcgctct tcttagctct aaaaacccag aaatccccaa tcgctacgcc
     4801 tgtaaaagtt gccttgagcc aattttttaa atatggacgt aactttccat atagaatatc
     4861 atttggtaaa aattcaatcc cttttctatt atcaaatttc tggctcacat ctttattcag
     4921 tctaccttct cctgccacga tatcttcaaa ttcaactttt ggaagtctac tggagtttgt
     4981 acttttatta agtctaacaa ctaaatcaat taacttacgc tcttcccaat cttccgtaaa
     5041 tcctgggaaa cggatttctg gtcgtgtatc attgctcatg tttgaacacc tccagcgcac
     5101 cttgtaacca catttcgttt tcaggtgttg tttgcaggga agagatagct tctaaaagtt
     5161 ctttttctag cacttgctgt tcttgtcgta tcttcttgat gtcatttccg acatgaacca
     5221 tatcaattgg ttcttcttct tcgaaagtat ctacatagcg tgggatattt aagttatagt
     5281 cattttcttt gatttcatca aaggttgcca aatgagcgta tttctcgaca tcttttcttt
     5341 cagcgtaggt ttcgaggatt ttttgaatgt tttcttccga tagtttattt tgatttttcc
     5401 ctttaacaaa ttcacgacta gcatcaatga ataatacatc tcgagtttga cgattcttct
     5461 tcaacacaat caccgtcgtt ggaatcgatg tcccaaagaa caagttcgca ggcataccga
     5521 tcacagcata aatactgcca tcttctagta atttttgacg gatgacccct tctgcagcgc
     5581 cacggaataa aactccgtgt ggtaagacga tggccatggt acctgtttcc ttcaaatgat
     5641 agaacccatg aagaagaaac gcaaaatctg ctttggattt tggcgctaat ttcccataac
     5701 gattaaaccg agaatcatct aagaaagtcg tatctgctga ccaattagcc gaataaggtg
     5761 ggttcattac tactgcatca aatgtgtaag gttcatctgt tggccagtcc ttattcaacg
     5821 tatccccatt gcggagattc atttcttcgg cgtcgacacc atgtaaaatc aagttcattt
     5881 ttgctagatt gtatgtcgtt gtatttagtt cttgcccatg atatttaacg ttatctgggt
     5941 gtgttaagta gttacgtacg tttaacatca atgaacctga acccatcgtt ggatcaaaaa
     6001 cactaaagaa tcgtcgttcc ttttgatcga gcgttacgat ttgcgccatc atatcagaaa
     6061 ccatatgagg tgtgtaaaat tctccggctt tcttccctgc ttctgaagca aattgactga
     6121 tcagaaactc atacgcatcc ccgatcacgt ccccatcgtg ttctaaaaca tctacatcat
     6181 tcaatttctt gatgacttcg gtaatcgtca catttcgttg ttgctcatct gttcctagtt
     6241 tttttgattg gagatccaca tcatcaaata acccattaaa ttgattgtag gtagatgcca
     6301 attgcacaaa ggctttgttt agatcgttca attggaacgt tgcttgtttt gcttgatccg
     6361 ctaagacgtt gaaaagatac tctggtgcaa ttgcatatcc caaaatatca acgattgtgg
     6421 caatcagatc ttcttttgat tcttcatctg aaagtaattc acgatacaac attgtttgtt
     6481 tgcttactgt gtcatattcc tctaaggatt catctgctaa aagaaccact tgttccaaca
     6541 atcgatcgga taaatattta tagaaaatca gtcccaataa ataattttta tattctgacg
     6601 catccatttt actacggaga ttatctgccg cactaaataa tttttgattt aattctgcac
     6661 ccaaaaagat acactcctat ctcttattta aagttgctat ataccctctt attttagcat
     6721 accccctttt ataaaaagca aaaaaaaaag aaagaaactt attcttgcta ataaactacg
     6781 cgaaaggagt ctaacgtt
//
LOCUS       DAMYXM010000102         6643 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.106, whole genome shotgun sequence.
ACCESSION   DAMYXM010000102 DAMYXM010000000
VERSION     DAMYXM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.106"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(1..859)
                     /locus_tag="PNS21_002596"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="PNS21_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="PNS21_002597"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="PNS21_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDI5160236.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="PNS21_002598"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="PNS21_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2603..3421)
                     /locus_tag="PNS21_002599"
     CDS             complement(2603..3421)
                     /locus_tag="PNS21_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDI5160237.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="PNS21_002600"
     CDS             3582..4271
                     /locus_tag="PNS21_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDI5160238.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="PNS21_002601"
     CDS             4285..5787
                     /locus_tag="PNS21_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDI5160239.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="PNS21_002602"
     CDS             5800..6276
                     /locus_tag="PNS21_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDI5160240.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="PNS21_002603"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="PNS21_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1031 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gttttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DAMYXM010000103         6357 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.107, whole genome shotgun sequence.
ACCESSION   DAMYXM010000103 DAMYXM010000000
VERSION     DAMYXM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.107"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(99..1229)
                     /locus_tag="PNS21_002604"
     CDS             complement(99..1229)
                     /locus_tag="PNS21_002604"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDI5160241.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="PNS21_002605"
     CDS             complement(1878..3080)
                     /locus_tag="PNS21_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI5160242.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="PNS21_002606"
     CDS             complement(3140..3343)
                     /locus_tag="PNS21_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HDI5160243.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="PNS21_002607"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="PNS21_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="PNS21_002608"
     CDS             4880..5692
                     /locus_tag="PNS21_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5160244.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1899 a         1302 c         1004 g         2152 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatg
//
LOCUS       DAMYXM010000104         6263 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.108, whole genome shotgun sequence.
ACCESSION   DAMYXM010000104 DAMYXM010000000
VERSION     DAMYXM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.108"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..504
                     /locus_tag="PNS21_002609"
     CDS             <1..504
                     /locus_tag="PNS21_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDI5160245.1"
                     /translation="SEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLTYKEGSAQ
                     VIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAKTPLQSEK
                     NIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLLLVLATFG
                     YAVLKFN"
     gene            complement(540..2597)
                     /locus_tag="PNS21_002610"
     CDS             complement(540..2597)
                     /locus_tag="PNS21_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HDI5160246.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(2742..2933)
                     /locus_tag="PNS21_002611"
     CDS             complement(2742..2933)
                     /locus_tag="PNS21_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160247.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            3121..3576
                     /locus_tag="PNS21_002612"
     CDS             3121..3576
                     /locus_tag="PNS21_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160248.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            3573..3815
                     /locus_tag="PNS21_002613"
     CDS             3573..3815
                     /locus_tag="PNS21_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HDI5160249.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            3833..4135
                     /locus_tag="PNS21_002614"
     CDS             3833..4135
                     /locus_tag="PNS21_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160250.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            4204..6258
                     /locus_tag="PNS21_002615"
     CDS             4204..6258
                     /locus_tag="PNS21_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160251.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
BASE COUNT         2159 a         1010 c         1277 g         1817 t
ORIGIN      
        1 tctgaagtac aatatctagt cacacaaaaa tggcacacaa aaggcgtaga cgctgcgtca
       61 gatcactaca aacaatttag tattcaagat ccaattgaag ctcgtttgac atataaagaa
      121 ggttctgcgc aagtaataga taagagtaaa ggaaaagata ttacttctga aggcacacta
      181 acctatgata gcaattcaag aacgctgaag tgggaagctt ccgctgactt cttaaataac
      241 aatcttttag atggacgaga aattcagctg atatttacag ctaaaactcc attacaatca
      301 gaaaaaaata ttgataacca agccgtagta gcagtagata atgtttctaa taaaacaaat
      361 gttgtgacaa ttggggttga tcctaacttg ccacaagtta ttgttcctaa aacaggttct
      421 acacatttag taacgatttt agcggtcagc ttactattac ttgtgttagc aacttttggc
      481 tacgcagttc tgaagttcaa ttaaacaaaa aaacaaggct gaaaatcagc cttgtttttc
      541 tatttgaaaa tgggattggc actaataaat acgttgtctg aagaaagtga tccgtctgca
      601 gaaacttcat aagcatggga taaaccaacg atactttgat tgttgtaaag caagtttgcg
      661 ttatgagcag cgctatcttt ccacataacc atagcattta ctgctcctgt atctgaaagg
      721 atctcgtcaa cgtaaaggta gaaactattg atattttctc ctttagcacc gtagcagcct
      781 aagctttcta agagatcttg gaaagtaccg ccgccaggtc ggtaatgact gaagcttact
      841 tgtaattctt ttgcacgaat atctgcagct tgttgaacga tatcattgaa ttgcattggg
      901 gcaacgccat tttgttgacg gtagctattt aaccaagcaa gttctttttg acgcacatag
      961 ttgtttaaat cacttagtga gatatttttg tatttgaaag tgatcgttcg attttggtca
     1021 acattattga ttacacgttg tccattttta tcaacggtaa cagatgaatc gtcatttcta
     1081 cacaagaagt agttttgatc ggtcatattt ttccaagaag tagagaaact atctccctcg
     1141 taaaccgttt gtgtttcagt agataaaaca gcgtttgtgt caacgtttat atggttcaca
     1201 gtgacgttgt attgatgagt ataggcaaaa ttgattcctt gattacttgt aacgttgttt
     1261 aaagtcacag tgttgctaga gctttctcta ggatcaagaa caaaattagt gaacataggt
     1321 gcttcaattg tgatgctttg tccataaacg acgggataac tcttactgta attatagagt
     1381 tcttggatat ttggatcagt atccgagact ttatttggac gagctttcac ttcaactaga
     1441 aagacttcat catagtaaaa agtaaatgtg gtatttgtgt taatcgtttg tgtttgtgtt
     1501 tcattcccat ttacttgata ctgtggtgct tcaaagttag tcaagtcggt tctagctttt
     1561 gcagtgtaac ttgtaccagc ttttactgag acatgttctt cttttagcag cgttccagtt
     1621 tgtcgattca tatgacggac agttaccgca tattctgaag gtttaggttt gtgttgtaac
     1681 cctttaaatg cagaatcaag actattttta gcattattta cttgggtttg agaagcttta
     1741 gaatcgtcta gtactttttt agcattggat aaagcagtct gaaaattatt ccaagatcct
     1801 tctgtaaaat ctcctttagc agttgatttt acttggttgt acaaggcttg taattccgtt
     1861 ttgttcacag taggagctgg ttttttcttt aatcccttgt aagcagaatc taatgtgttt
     1921 ttggcactat ccacttgttt ttgagtcgct tttgagtcat ttaacacttt tttagcattg
     1981 tttaaagcgg tttggaatgc gttccaggta ttgtcggtat agtcaccttt tttagttccc
     2041 ttaacgctcg tataaagagc ttctaaagct gatttattca ctttgggttt agaggtgtca
     2101 gcaccatacc aaccaatacc ttcgtatttc cagccatagt ctttggttaa ccattgtact
     2161 tctttccaac ttgcagtgta gtgatgcgaa ccagaggtaa cacctggaag gaaaaggcgg
     2221 tagatcggtt tattattttt ttcggcagaa cgccaggcag gcccttcata agtccaaccg
     2281 taatttttgg tcaaccattt cacttcatcc caactagctg tgtaatggtg atcacgaagg
     2341 cctttattgt ataaacgata gacaaggtgg ccaccgctag tcggtgcttc ccaagcgggg
     2401 ccttcataat taccccactt aattttcact aggtgatcac gttcttttgt gctagaggtg
     2461 taaaaatgtt catggttatt tggattataa aggcgataca tgacgttagt tgaagcagcg
     2521 tcagcagatc cagcaagagc aactgtggca gcaaatgttc caatagctat aacagttttt
     2581 gggtttaata atttcatgtt ttcaactccc tattaaatga ttaaatattt tgtttacatt
     2641 cataatatac cacggtatac cgaggtagtc aacggtatac cgtggaaatt ttgaaataaa
     2701 aaagccgccc tatgaagagt gtaaggatgg ctgtttatat gctatgaaga aatggattcg
     2761 gcgtatttgg ttagtttggc agcattatca aatcgcattt tttcaatcgc actttttttc
     2821 tttattaaat ccgcaattgt tgattctgcg actgaagaat gtttgctaat ttcatatgct
     2881 gtcttgccag tttcaaatag taaccattcg attttctctt tatcagcgta cattgcaatg
     2941 ctccctcttg attttttctt tattatagct cttttttgta ataaatcaca atatcaagag
     3001 gaaaagtttc tatagaaagg tgggaaatta tgagtatccg ttccccgtgt atcaaaatga
     3061 ggtttgttgc tactttcgtt atttaatata ctaaaataaa acaacgaaag aaggaataga
     3121 atgaaaaagt taataatggt atttacttcg ttgattgcca tgattgttat tttaggtgga
     3181 tgtggtagtg aaaaaaataa gcaagatagt caagataatg gcgtgttaaa accaggaaca
     3241 gtgtggaaag aagaagtagg cggacttgtt cataatttaa aaattataga tgatacaaca
     3301 tgggaatatt ccgaaagtgt ttggcatcct gaacaagtac aaataacagt tgagaagcta
     3361 aaagactata aaggattaga aagatataag gtaatcgatt caggtggagt agatttattc
     3421 ataggtaaaa cagccttaat agttccctat aacaaagacg gtttagaaac gtttaaattt
     3481 attccaacaa acgaaaaaga acatcgagat aaaagtgaaa tattaaaaga atacagtact
     3541 aaatctggat acaagttaca aaagactagc gagtgattgc tagtcttttt atttatcctg
     3601 aaggaggaat cgcagtggca aaagctaaaa cgccatttga aaaagccgcg tctaaaatct
     3661 tagaacgtcg cggtgtatct gaaacacagt ggaaacaaga agtccttgaa aaagcacaat
     3721 ttgacctatt acggggaaaa gacaaggact tagaaggata tatctatcaa cgtgaacgtg
     3781 aaaagcttgt tttcgatgaa attattaaac actaaaagga gcgaacaatt ttatgtttga
     3841 atcaattaca atgaattttt taacacaacc gaaagtagta gctgcggttg atatttttag
     3901 agcattacaa ggtgcagcaa ctagtttaaa aaacaatctg atgatttttg cggctgtagc
     3961 tgccgttgcc tccattgttc ttggggggat tttcttcttc tttggccgaa gaggagctga
     4021 aaacgggaag atgcacgtag gttatgtatt gatgggggta ggagttatat tagctgccgc
     4081 ctctattgtg gtttcgttat ttggaatgtt tggacaaacc ccacagggac tataaaaaat
     4141 aaatagaagg agtaaaaagt acgtggctac tttttactcc ttttttatga aaggagaata
     4201 ctcatgtact ttacgatttc aggcaatacc aaaaaagata aagagttaat aaaaaaagca
     4261 acgaagaaag tgggaaagcg tactgccttt ttaccgaaaa caattaaata ttctgtcatt
     4321 gaaacggaag aacagctaca tgtgttagga caagtgttag aacaagagaa tcaatcttct
     4381 gttaaattga ctctcacgtt acatgataaa gaaggaaaaa tagaaaaggc tggcacagaa
     4441 tacgtttttg gcccactgga ttatcaaatt tcagaaggtt tttatcctac attgatggat
     4501 ttgatccgcc ttgatgtgtt taaggaaacg gatgaaacca tgagtgtgga agagtcttat
     4561 cgaaacgaaa aactgctaca agttttagaa gaagagttag agagcgacca aagaaaagtg
     4621 agtcaagtga gtgaagtcga agtaaatgat gaagtaccat gggaacgacc aatctttcca
     4681 aagaaggagt caaaagaaaa agagacagtc tatgttgacg aacaagctga tattccgact
     4741 cagaaatcga tatttgagga agagaacgat gtaatgggtc aattggaggt agatcccttt
     4801 gaagaaacaa tggaggatga agagacggcc gaagtagaag ttgatctagt tgaagctaat
     4861 gaagagccaa catggactga aggggattct caggaatata ttgaaccact tatcagtgaa
     4921 gaagtagaga aaaacgaaac agagatacaa aaggcaactg tatttgatta tattttggat
     4981 caatatccgc cagagcatga atggatgaaa gaaaaaaata aattattttt aaatgagctt
     5041 tattcgaaat atcaattacc ccaaacctat caagcatttt tagagagtaa agaagaatta
     5101 atctcacagg gtaaaggcac acttttagaa cagttagagt ctgttcaatc aacaaattgg
     5161 catgagcttg cacaagaaga tttattagcc acttttgaag aacgtcaaca agaaacagat
     5221 caagaaataa atgagtatgc gacggaacag tcatctaatt gggagagaac aaaagaggaa
     5281 ctaaataaag aagaacaacg agtgattgaa gaagaagtac aaaaaattaa agagcgatac
     5341 gaaaagaaac gcgacaatgc gtattccttg tgtatagaga aaattaataa ctttactaaa
     5401 actaatcaag aagctttatc gaaagaaaaa gaaaacttgt taaaagaacg tataaaagaa
     5461 cgaaaagaag agttctaccg aggtttacgt acagataagc taaagattag caaagaatta
     5521 aatgaacaac tgaataaact ctatcaaact acgactgaag ttcttatgga aagacacggg
     5581 cagatccagc aagaattaca agcacaaatt ccaaaatgga aaaaagagta tgaaattcaa
     5641 caacaagaat tgaaagaaca agaagaaaaa caacgacaag aagaaaaaga gcgcgaacaa
     5701 ttagaactaa aaaaacgtga gcagcatctt gcagaacaaa aagtggcgat agaaaaacaa
     5761 cggttagagc aagaaaagga aaaagaagca gctgctcgta aggaacgtca agaacaaatg
     5821 gatacattgc gacaagcaca gttgaattat ctagcacaac ctcaaccagt agctgctgtt
     5881 caaacgccag tgaacaacca agcaaattta actccttcac cacagcgttc ctttaacgga
     5941 tggatggtgg gatgtattgc aagtttaagc ttgcttctag gtggaggaac agtatttgct
     6001 tttaatcata tcaatcaatc ttcacaggca gattctgtag ccacaacggc gtcaatcaag
     6061 gaagaagccc aggaacaagc aagaaaagag tatgaacaac aattggaaga attattgaat
     6121 cgtaccaatc agtcaacagc agagtcaagt atggaaaata gcaaagagag tgatcctagt
     6181 aaggctaaag aaggatcaag cgattcaact gtacactcaa acacttccga aagttcagaa
     6241 tcaaatgagg tgaaataatg cgt
//
LOCUS       DAMYXM010000105         6183 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.109, whole genome shotgun sequence.
ACCESSION   DAMYXM010000105 DAMYXM010000000
VERSION     DAMYXM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.109"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            725..811
                     /locus_tag="PNS21_002616"
     CDS             725..811
                     /locus_tag="PNS21_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDI5160252.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="PNS21_002617"
     CDS             complement(932..1621)
                     /locus_tag="PNS21_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160253.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="PNS21_002618"
     CDS             complement(1628..1906)
                     /locus_tag="PNS21_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160254.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="PNS21_002619"
     CDS             complement(1866..2780)
                     /locus_tag="PNS21_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDI5160255.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="PNS21_002620"
     CDS             complement(2762..3142)
                     /locus_tag="PNS21_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDI5160256.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3602..3889)
                     /locus_tag="PNS21_002621"
     CDS             complement(3602..3889)
                     /locus_tag="PNS21_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDI5160257.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3909..4133)
                     /locus_tag="PNS21_002622"
     CDS             complement(3909..4133)
                     /locus_tag="PNS21_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HDI5160258.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4588..4758)
                     /locus_tag="PNS21_002623"
     CDS             complement(4588..4758)
                     /locus_tag="PNS21_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160259.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4773..5306)
                     /locus_tag="PNS21_002624"
     CDS             complement(4773..5306)
                     /locus_tag="PNS21_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDI5160260.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5327..6169)
                     /locus_tag="PNS21_002625"
     CDS             complement(5327..6169)
                     /locus_tag="PNS21_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDI5160261.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1637 a         1154 c         1043 g         2349 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ctcactaaag ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca
     3301 aataaccgct tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc
     3361 acaatacaaa aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc
     3421 ttcggtgaaa ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa
     3481 gattcttatg ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa
     3541 tttatgggac tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga
     3601 attaactcca aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa
     3661 tttcttgtaa ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc
     3721 caagaaaact acttaatttc atactaactt caccaacact ttttccctca ctgatttctt
     3781 tagaataaag aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt
     3841 atactttact aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct
     3901 ccctaccact accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt
     3961 tttccaattt ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa
     4021 agaccatttc catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga
     4081 caagttccta atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt
     4141 tttccttttc gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat
     4201 ataacatttt attatataac aatgattaac gtcataaaaa gtagttttga attcataatc
     4261 gtttaaataa aattataaga tcagttattt aaacagctag tagacataat gtgtatacta
     4321 ccaagtaacg cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag
     4381 gttaaaagct ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa
     4441 tattgatcgg tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac
     4501 atatctcata ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga
     4561 aagactgctc gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc
     4621 attttgactg ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt
     4681 ggccactgat aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag
     4741 caagtgtttt tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc
     4801 caattctttg tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc
     4861 tctgaagact cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg
     4921 ttggcttgca gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt
     4981 tgctctttga gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc
     5041 aacttgtttg caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca
     5101 tgtttatgca acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact
     5161 ccagaacgca atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat
     5221 ttttggatat gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt
     5281 attgtaaagg tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat
     5341 tatctaaatc agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt
     5401 tttcaagagc ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc
     5461 gtttcttctg tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt
     5521 tatcagctaa agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt
     5581 cgtctgcttt tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc
     5641 gtccttttcc gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac
     5701 gtataggttt tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta
     5761 ataattcaag aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta
     5821 acagacgaaa catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat
     5881 agcgtaccca ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat
     5941 ctacgtaagg attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat
     6001 cgtaagaaag cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag
     6061 ttgcttttaa atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt
     6121 ttagttggtc aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga
     6181 tgg
//
LOCUS       DAMYXM010000106         6135 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.110, whole genome shotgun sequence.
ACCESSION   DAMYXM010000106 DAMYXM010000000
VERSION     DAMYXM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.110"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(207..1169)
                     /locus_tag="PNS21_002626"
     CDS             complement(207..1169)
                     /locus_tag="PNS21_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI5160262.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="PNS21_002627"
     CDS             complement(1171..2610)
                     /locus_tag="PNS21_002627"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDI5160263.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="PNS21_002628"
     CDS             2795..4741
                     /locus_tag="PNS21_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HDI5160264.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /locus_tag="PNS21_002629"
     CDS             5005..5877
                     /locus_tag="PNS21_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDI5160265.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1946 a         1053 c         1085 g         2051 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaggc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa acgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacaaaaa aaagggggtg cggatatttt tttggacctt tttaacaaat
     6121 atatctttgc ttctt
//
LOCUS       DAMYXM010000107         6062 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.111, whole genome shotgun sequence.
ACCESSION   DAMYXM010000107 DAMYXM010000000
VERSION     DAMYXM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.111"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            642..1376
                     /locus_tag="PNS21_002630"
     CDS             642..1376
                     /locus_tag="PNS21_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI5160266.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1392..2561
                     /locus_tag="PNS21_002631"
     CDS             1392..2561
                     /locus_tag="PNS21_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDI5160267.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2577..3572
                     /gene="lacD"
                     /locus_tag="PNS21_002632"
     CDS             2577..3572
                     /gene="lacD"
                     /locus_tag="PNS21_002632"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDI5160268.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3651..4577
                     /locus_tag="PNS21_002633"
     CDS             3651..4577
                     /locus_tag="PNS21_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HDI5160269.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4596..5846
                     /locus_tag="PNS21_002634"
     CDS             4596..5846
                     /locus_tag="PNS21_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDI5160270.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2036 a          992 c         1234 g         1800 t
ORIGIN      
        1 agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt gactaaacga
       61 agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat tcagataaac
      121 tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc ttaagagaga
      181 acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat ttattctcat
      241 agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga tttgaaacca
      301 aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca ctggtaagta
      361 gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta aaaaaagata
      421 ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa aagttggaaa
      481 attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat attaacaaga
      541 ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa ataccactac
      601 tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat acctttcaat
      661 caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa aacagtatgg
      721 tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc gttagtcgca
      781 cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat cgacaacatg
      841 gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct ggcgcatata
      901 gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata ttggccttca
      961 agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta ggtgagcctt
     1021 tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc gagaaatcct
     1081 atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg aaacctttat
     1141 ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag gaattttatg
     1201 ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga tcaccagtgc
     1261 tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt acattaagtg
     1321 tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact aactgaattg
     1381 gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga gcagaaaata
     1441 ctacccgaga aattcgacaa caaccggaac tgtggcatga gacgtttgac cgttatcaag
     1501 aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct ggagagcgac
     1561 caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat acactaattc
     1621 cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt ggttcgaccg
     1681 atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc ttggtctcat
     1741 ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc aatcagctgg
     1801 tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg gctacacaaa
     1861 tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat gacgctggtt
     1921 ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt tttgatcaag
     1981 caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga gaagaagtca
     2041 tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc atcatttact
     2101 taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt ctcgagttaa
     2161 ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat ggtcccaaat
     2221 cttttgtcaa tgagcagaca ttggttttcc tgtttgtcag caatgactca tatacgcaga
     2281 aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa cgcgttgttg
     2341 gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt gcagattcgt
     2401 tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg atcgcattac
     2461 tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga acagtcaatc
     2521 gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg tgaagcatgt
     2581 tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca gaaggcatta
     2641 ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg gctttaggga
     2701 tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa gaattgactc
     2761 cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca cagaagcgtg
     2821 cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa accgtacccg
     2881 gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag ttaggtgcgg
     2941 atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc aatcggcaaa
     3001 agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg ccattttatt
     3061 tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc tatgccaaag
     3121 caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag tataaagtag
     3181 atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat gcaacagggg
     3241 agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc gatgcaacag
     3301 atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg gagacattgc
     3361 gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga gcaacttgga
     3421 aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa tggctgacag
     3481 aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact gccatcccat
     3541 ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt agtgataaga
     3601 gatatgtgtg attataattt atcattattg aaactgggag gatattttat atgaataaaa
     3661 gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa ttaaagcagg
     3721 caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt agctgtcctt
     3781 acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct gatttaattt
     3841 tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta actctaaaag
     3901 agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta aatttagaac
     3961 ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt ggtagaatcg
     4021 tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt tgtttaactg
     4081 gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca ttatccaaat
     4141 caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt gatgaaccaa
     4201 ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt ttattagttc
     4261 ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc gttgattata
     4321 taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta acggctaatg
     4381 gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta gcatcaaaag
     4441 catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt cttccagaaa
     4501 atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt atgttaggaa
     4561 aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt caaaaaatag
     4621 ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg gctgcagtta
     4681 tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg atagaaggat
     4741 ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca tcagattcaa
     4801 catttggtat tattggatta gctgttacct actctttggg acacaatcta gcaaagactt
     4861 atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt ttacttacac
     4921 ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt acctcgggtc
     4981 tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg tttgtactta
     5041 aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga gctttcgttg
     5101 ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta atttgttata
     5161 aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa ccactatctt
     5221 tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt ttcttttgga
     5281 tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta ttattgcaaa
     5341 actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac attataactt
     5401 atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt gcacttgcca
     5461 ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag ttatcaatcg
     5521 ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact atattaaata
     5581 taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt acttattttt
     5641 caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg acaactccac
     5701 cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg attcaagtaa
     5761 taacaattgc aataaatgtt ttgatatact atccattctt taaattagat gataatgcca
     5821 agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt agttaagtat
     5881 cgccgtttgt ataattaagc gagacaaata aaaaagccat ttatgttaca ctccaattgt
     5941 aatacccgct aaagcataca aggagagtga acataaatga cttataccca tcttacacca
     6001 aacgagcttg taatgataga agcatatttt catcaagaaa ctccggttgc tatcgttgcg
     6061 aa
//
LOCUS       DAMYXM010000108         5863 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.112, whole genome shotgun sequence.
ACCESSION   DAMYXM010000108 DAMYXM010000000
VERSION     DAMYXM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.112"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(103..2007)
                     /locus_tag="PNS21_002635"
     CDS             complement(103..2007)
                     /locus_tag="PNS21_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDI5160271.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISVGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPTEKVKIS"
     gene            complement(2205..2438)
                     /locus_tag="PNS21_002636"
     CDS             complement(2205..2438)
                     /locus_tag="PNS21_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDI5160272.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            complement(2585..3439)
                     /locus_tag="PNS21_002637"
     CDS             complement(2585..3439)
                     /locus_tag="PNS21_002637"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDI5160273.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            complement(3424..3762)
                     /locus_tag="PNS21_002638"
     CDS             complement(3424..3762)
                     /locus_tag="PNS21_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160274.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            complement(3990..4694)
                     /locus_tag="PNS21_002639"
     CDS             complement(3990..4694)
                     /locus_tag="PNS21_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDI5160275.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            complement(4839..5411)
                     /locus_tag="PNS21_002640"
     CDS             complement(4839..5411)
                     /locus_tag="PNS21_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDI5160276.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
BASE COUNT         1672 a         1326 c          985 g         1880 t
ORIGIN      
        1 tcaagaactc aaaaaagcgg aaaatcccag atttaaaaat ctgagtttcc gcttttgtca
       61 tttcaaggat ggactattgt ctcagcctct tctaatttat tcttaagata tttttacttt
      121 ctctgtaggt gctgatactt ctttttgttc tttccgacct ttcaacttat acttcattaa
      181 tctcatgcca ttgaaaatta ccaccaagat actaccttca tgaaccaaca ttccgataga
      241 catattcatc cattcgctga agaagacgct ggtcaacaag accaacacta ctccaactga
      301 aatcacgata ttttgtttca tattatttgc ggtggctttt actaatccta atgcgtgagg
      361 caagttgctg aagttcgagt tcattaaaac gacatcggat gtttcaatcg ctacgtccgt
      421 tccgcttccc attgcgattc caatgtctgc taaagctaag gacggactat cgttaacgcc
      481 atctccaaca aaggctacaa tttgaccacg ttgttgaagt tttccgatat aagccgattt
      541 gtcttccggc aacatgtagc cgtgagcttc ggtcaaacca agttcgccgg cgaccacatc
      601 aacggttcct tggttatctc ctgaaagaac gactagattt ttcacgccca agtcttttaa
      661 ttcctgcaaa ttagctttca cacccggacg gacttgatcg cgaatgccca tcagtacttt
      721 taattcaccg tcgaccgctg tcaaaacgag agagttccct tgttgttcaa accgtttgac
      781 atctttttgc acttttttgc tgagagtgac attttctttt tccatcaagg ccacattccc
      841 aacagccact ctatgtccag ctacacttga aacgattccg ccgcctttca caacttcagt
      901 tccttcaaca ggataaaagt ttgtttctcc aatttgattt aagaccgctt ttgctaatgg
      961 atgatctgat tctcgttcca cactggccag atagccaagc gtttcagcag gatctttttc
     1021 atacagttca gtcgctgcaa cggttgggtt tccgacagtt aaagtacctg tcttatcaaa
     1081 tacaattgta tccacattac tgaagtcttg aatgacttca ctccctttta aaagaacacc
     1141 gttacgagca ccattcccaa taccggcaac gtttgagaca ggcactccga taactaatgc
     1201 gcctgggcaa cctagaacca agatggtgat tgctaactca atattttgac tgaacgccca
     1261 tacaacaatt gccagaacca agacagctgg tgtgtagtat ttagaaaagc gatcaatgaa
     1321 ccgttccgct tcggatttag aatcttgtgc ttcttccacg agttcaataa tttttccaaa
     1381 tgtggtgtcc tcaccaactc ggtcagctct gatttgaatc gttccgtttt ctaaaatggt
     1441 tcctgcaaaa acttctgcat cagctagctt gttgaccgga atagcttcac ctgtaatgct
     1501 agcttcattg atatgccctt cacccgtaag gactatgcca tctactggaa ctttcgcacc
     1561 cgttttaacg agcaagatat ccccttcatc tatatcatcc acttctactt cttcaaattc
     1621 gccattatcc atttgtttca aggcgctttc gggtgccatt tcagtcaatt ctttgatagc
     1681 agatcgcgtt tggttcaatg ttcgttgttc caagtagtgt ccgaataaga ataagaacgt
     1741 gacaatagcc gattcttcat aattttgaat aaaaagcgca ccaatcgctg caatactgac
     1801 taagacatca atactgatga ctttgacttt cattgcttga aacgcttgaa tggcaattgg
     1861 cgtaatccca aagacagagg cgatgatcaa agaccactct gaaagtccta cgttttctaa
     1921 aacaaagtga ctgaagaaac cgagtacgat taacaatcca ctgatgacgg tgataacatt
     1981 tttcttgctt aatatatatt gttgcatgct ttttccctcc aaatttaatt tctcttttat
     2041 tgataaacac agtataagat aattgcctaa gtaattaatt gactgacatc aagtttagaa
     2101 agttctcttt tatcgtttct aagcacctcg aatcggattg caacaaaaaa agcaatcctg
     2161 gaaaaatagc ttttcttcca gaattgccta gttcatttaa tctgttaaac ggccttgact
     2221 tttgctttaa gaaccggata gcctacgttt tcgatagctt tttgaatctc ttcaatcgaa
     2281 gtgacagctg aatcaaaatt ggttttgact ttgctggagt tgaataatac tttaatgctg
     2341 tctttatcaa taccgttaac tgatttcacg gcaccttcaa ttttttgcat acaacttgga
     2401 caagacaacg tttctaattg caatatagct ttttccataa ttcctatctc ctttatgtgt
     2461 ttttatttta tcggcagcag attaatgaac actgcctttt cctctttaca actgtagtat
     2521 agctcacttt tagcaaacga aaattgacgc atatcaagtt ttgcacttct ttttatcttt
     2581 ttgctcaatt caccatctta atagtctttt tctgcccctt attgccatca gaataataag
     2641 aaattggtgg ataatcttgt ctgcgtataa accatactac aatcgcgcca ataaacaggg
     2701 ccagagacaa ggcttgcgaa acgcggatcc actcgccaat ccataaactg tccgttcgca
     2761 taccttcaat gaagaaccga cccactgagt accacaaaac gtaactcaaa gcgacttctc
     2821 cttgacgcaa aagctgtttt cggttccgta tagtgacgat gaggacaaag cccaacaaac
     2881 tccacaatga ctcgtataaa aaagtcggat ggtagtaggt accattcata ttcatttgtt
     2941 caatgataaa ttcaggtaaa taaagatttt ccaagaattg acgagtaacc ggcccaccat
     3001 gagcttcctg attcataaaa ttgccccaac gaccaatgga ctgggctaac aatacattgg
     3061 gcgctaaaat atcaagaacg agtgccaaag aagtaccttt tttctttgca taccaatata
     3121 gcgtaccccc tcctgcaatc aatcccccat aaatcgcaat gccgccattc caaatcgcaa
     3181 ggatttgtcc tggattttgc agataataac caagttcaaa taacacataa taaagccgag
     3241 cgccgaggag tccaatcgga attgcccata aggccatatc gataatggta tcctcttcca
     3301 gtcctttttt ctttgcttcc ttggtgctca actggagagc cgctaatatg cccgctgcaa
     3361 taatgactcc ataccaatga atcacaacag aaccaatctc caccacatgg ggattcaaaa
     3421 cgcctaatac gaatcccatt ctatttgctc catttcttca atttttcgcg tcattctagt
     3481 catggtgatg ctgaacaaga ttccggctaa gctccatgga aaccaaacag tgaatacact
     3541 tttatctccc tggtcactcg cttcgtaata cgccactttt ccgtgtccga ttacgccggc
     3601 tccttttaca acttcctctt ctttttccat agctaaccta tctgcctcaa caacgttaga
     3661 cgtttttgct tcgccgccaa tcgtgttctt ggtgaaaggg acgacaaaga atgcccccgc
     3721 tgctgacaac gtgatggcaa ctatcaccca aaaacaatgc acattcaccc tacttatttt
     3781 tacgttttgt tcgacaaccc tatcttaaag aaaaataaaa caaaaaaact tgagggttat
     3841 catgtttgtg cttttatttt tccaatgaat gaaggtcgtt ctgtcttaaa aaagccaaaa
     3901 acggacagat ggaagcaaaa gcactgagtt ctgtcccgtt gttaatgaag agcgacagaa
     3961 caccaacgtg tagaaaaaag tgtgtccgat tattcgctgt aaagcaataa atcatctaaa
     4021 tcctgaatct tgatcctttt cccagacagc tgttgaatca atccttcgtc ctccaattct
     4081 ccaaatttgc gactgatggt ctcaggtgtc gttcctaaat aggaagcaat gtcctttttt
     4141 gccatcggca gggtgatggt gggagagttg cccatttcag gttccacatt ttctgccaaa
     4201 aacaaaacta aacgggtaat gacactctcc acggctactt gtgtcgtttg acgttctgaa
     4261 ctttctaacc gtttagacat ttccgccaat agttttaaag aaatagctgg gtattgttcc
     4321 agaaaatctt ggacatcttt ttgctgaatc atacagacaa cggtatctcg ggttgcttcg
     4381 gcatattcct cgtgtactgc atccgggttg aacaaggtcc attctccggt gaaatcacca
     4441 gggttcaaga tccgcactag ctgctctttg ccagagtctg ccaatcgata aatacggatt
     4501 ttcccacgat tgataatata taaagtatcc tctttttctt gtgaacgaaa cagaaactct
     4561 cctttttgat actgtttcgt gtgggctttt tcagcaatgt aattcatcgc gctatcctct
     4621 aagtgattga atattggaac caagcggata caggctgcgt ggtcgccaat ttcatgatgg
     4681 gtatgtttag tcaattcatt ctcccccttt atttattttg tttatattcg ctgcttttat
     4741 tatataagtc tcttactaca aaaaaattga tgcacatcaa gaaaatggaa aaaggactgg
     4801 aaagaattcc aatccctaaa tcagcttcta cttttacctt aatcttcatc ctcatcaaga
     4861 gcgtcatcat cttctaatgc ttctttacca atgaacgctt gcagcatcca aatgttcttg
     4921 tctaagtcat ttttaaaagc aatcaatgta tcttctaaag catcgtcacc ctcgtcttga
     4981 gctaaacgaa tcgcgcgaat agttaggtca cgagtgcttc tgaagtcctc gataatattt
     5041 tcaaccatgt cttctgcttt agcgtattta ttaactgcat cttccgaaag catcgaatat
     5101 ttttcaaatt caaccgttgt cgaagcaggt ttatgcccag aagtaattag acgctccgca
     5161 agtttgtcga accatttttc attttcgtta tacaactctt caaatttttc atgcaacgta
     5221 aagaaattgg ctccttttac ataccagtga tattggtgca gtttcacatg taatgtatgc
     5281 atgttgccta aaagatgatc cgcaatggcc gctgcattaa ttttcgtatg atgaacatgt
     5341 tccttgtgtt cttgttcttc ttgcaatctt tcttggggtg ttctttcttg agtagcttta
     5401 ttttcagtca tgatttattc ctcctataat tttggtttgt ttaaaatagc tttctttgat
     5461 tcctcctttt tctttactga atttagtata ggatagattt ccgttaaaaa aattgacgct
     5521 catcaagaaa attatttctt aatagacgcc aggcttctct attaaattca actgaaataa
     5581 tcaaaggaat gaagtcgttc cctccaaaca aaaatttcat taaagtaggg cctaaaatgt
     5641 catttttata cttaattggc acagtttttt ctaattattt gtcatattca tagtcaacta
     5701 tagctacaaa aacaactaat tttggagata gattgagata tttaaaaatc tctcatgaaa
     5761 aattagttgc ttttgtcact tagcgctgaa aatttatgcc atttatctag aaaaatgaca
     5821 accatgtttt tcaatgttat tatgatgtct atttagggta tac
//
LOCUS       DAMYXM010000109         5767 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.113, whole genome shotgun sequence.
ACCESSION   DAMYXM010000109 DAMYXM010000000
VERSION     DAMYXM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.113"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(240..947)
                     /locus_tag="PNS21_002641"
     CDS             complement(240..947)
                     /locus_tag="PNS21_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160277.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(1012..1848)
                     /locus_tag="PNS21_002642"
     CDS             complement(1012..1848)
                     /locus_tag="PNS21_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDI5160278.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1866..3275)
                     /locus_tag="PNS21_002643"
     CDS             complement(1866..3275)
                     /locus_tag="PNS21_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDI5160279.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3289..5112)
                     /locus_tag="PNS21_002644"
     CDS             complement(3289..5112)
                     /locus_tag="PNS21_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDI5160280.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1767 a         1256 c          944 g         1800 t
ORIGIN      
        1 ttaatgaaac tatctccgtt tcatttcaga tacaaataaa tatagtcggg ctccaaatcc
       61 gaaaaaaaat acccccagta gcttatccta agctactgga gatgtataag tacggtcata
      121 taacaacggg gtagggtaaa taaatgaaaa agatggaaga atcattattc aataactttt
      181 ttaaatactt cgttgtaatc agttacctgt ccgttcttaa ataccacaga ataatctttt
      241 tacccatttt caagtaaccg aagtggaatg tctttaccaa cttgctctgt cactgcatta
      301 ctgatacctg ttagattagc taataaatct gataaagatt gagcatctgt agaaatctct
      361 gtatcagtat ttatcgtgat tcctttatct tcatttacaa gcttccaagt aggtggtata
      421 gctgtgtatg tttcgatgaa tgaatcaatt ctttcatcta tcgtcttccc agtatctact
      481 tctgtctcat tactcttaga tgaatcgatt ctttcatcca tcgtctgctc cgtaactgct
      541 tctgtctcat tattctcacc ggaaactctc ttcacaccca aacttatata ttgtccatct
      601 ttagtgtgca tagttaaaat tcccacgctt ttactaataa attcactata accagttctt
      661 tcaatagaga cttcaatgta tttcacacta tcatactcaa aagaaaaata gacatgtaaa
      721 agcattttgt tgtttacatc tacataataa tcctttgact ctacttgaga ccttcctata
      781 cggaatacag ctttactgtc tgatatctct ataccagcat ccttattatt aatctgccaa
      841 tctccaagta gctcttctgg caagctagta gtttcttgtg aacactttac cacttcactg
      901 gtagaggtcg aaacattcac ctccgcactg ttagaagaat tttccaagga atcaggagcc
      961 ttagcacaac caacaagtct ttatatattg tgcagccgat attttcttca ttcagtacac
     1021 ccttgaaata tgcagtgtta gataaagcag ttcgtcctga tttgctgagg cacccatctc
     1081 ttcttgaacg tactccttga ttttcaaggc cgcccgatac tctttcggaa aacgctgctt
     1141 cactgaagcc agcaaaaaat catccttctc cggcaactct tccttcttaa tcattcgggc
     1201 aataaaaaac tgtaaatgcg tcacaaatct taagtaaagc atcgaatctt tatcaacctt
     1261 tttttcggga aacagatatc ctagcatagc caagatatcc tttagtgctc ttaccgagag
     1321 catggtttct tccatatttg cttgttcact ttgggcattt acaatgtgaa acgcaatgtt
     1381 tccagcctcc tcttctggca actgaacatt catttttttg ttgatcaatt ggatagcata
     1441 gcagccgatc tcaaattctt ttgggtaata cttttgaatt tcaatcagca ggcgattttg
     1501 aaaaacaatg cctttttgac tgcgttcaat ggcatgcttc aagtgatcaa ccaaggtaaa
     1561 aaatagactt ttattcagct taaagtcaaa cttggcgttg gcataatcga taattcgctt
     1621 gacgcaaata attagatcat ccggagcagc ctcaaacagt ttaatgtaat ccttttttag
     1681 agcatcagaa agtgaatcta atttaaatac tttttggatt ttgtcagccg gaaccaaatc
     1741 gcctttcttt agttgaaatc caatcccttt ccccatcaaa attacttctt tgccttgctt
     1801 atctaagcaa agaattaaac tgttgtttaa cgttttaatt actctcatta gattcaccgt
     1861 tctctctaag ctaagccatt tcttgcgatt actttttgat accaaaagaa gctttctttt
     1921 tttattcgtt ttaattcttt aagattctca tcggttcggt caacatatat aaaaccataa
     1981 cgcttattga agccttgatg ggtactaaca acatcaatcg ctgaccaagg taaataacca
     2041 aaaacatcta caccatctgt taaagctgct tgaatagcca gaatatgttt ggctagataa
     2101 tcaattcgat cctggtcatt aattcgtcta tcttcctcaa ctatgtcggg tgccccatag
     2161 ccattttcag taattaaaat aggtaaatca tagcgatcgt acaatttcct caatgttaat
     2221 cgtagtccag tagggtcaac tacccagcca tatggcgttt tatctacata aggattttca
     2281 gctgcccggt agacaccttc ttctcccaac atgatctgtt gatcccccgc tctagcggaa
     2341 acatctgatc cgtcattttt gctggcagcg atggtggcag ttgaataata attaatcgca
     2401 ataaaatcgg gtttaccgga ggataaaatt gcctgatcct ctgcctcaat tttaggatat
     2461 aatccacgat cattcaaata gttttcaaac aaataattgt attttccccg aactgctaca
     2521 tctaagaaag accagcctct caatgtttcc caattatggg ctgcaattgc atcttctgga
     2581 ctggaggtag cttgatacat agccgttaaa ttgattgctg gaccgatttt tcctttaaga
     2641 ttcaactgat gatacaagtg aattactcta gcttgagcta ataaaacatg gtgattaatt
     2701 tgaaagagtt ctttttttga aggcagctct ccacttggtg gcaaaccaat cgctccagga
     2761 tgaaggatca tcgtgttttg ctcattaatt gtcagccaat agttcacctt gtcaccatac
     2821 tcagtaagca atacctgtac atagtccaca aaataatcga tagttgctcg atttaaccaa
     2881 ccgccctgct cctctagaaa ataaggtaaa tcgaaatggt acatggtgac aatcggttcg
     2941 attccctgat tcaggcattc atcaattact tgatgataaa aagaaattcc cttttgattg
     3001 gttttattat tttcaatatc cataattcgc gtccaagcga tggaaaagcg aaaagctttt
     3061 aagcctaatt ctttaaacag ctgtatatct tctttatagt gatggtaaaa atccgaggca
     3121 acttcaaaat tagctacccc ttctgtatgc tgatacttgt caataataga cggttgtttc
     3181 ccatcctcct gataggcacc ctctgcctgg taggcactaa tggaggcacc ccataaaaac
     3241 ttctctggaa atgctgaaag ctgtttgtat tgcacaaaaa tcctctcctt aactaatttg
     3301 catcaatggt tcgtgttcct ttaagcttcc gcttgcttca ctaattcctg cgtaattttc
     3361 actgttggtc actaccacaa tcactgtatc atcgtagcca gcgtttctaa tggcagcgaa
     3421 atcggcgttg attaataatt gtcctgctga cacctgatct cctgctttta caaatacttc
     3481 aaagggttcc ccttttaaat tcacggtgtc gatccccaca tggatcaata attcaatacc
     3541 ttctttagaa cgtagaccaa tcgcatgttt agattctaaa atagtaatga cttctccctc
     3601 aacaggtgaa taaatgctgt tttcttctgg atgaatggct acgccctttc ccaatagtcc
     3661 cttagaaaag acagcatcgt tcacttcaga aataggacac acggttcctt ttaccggatt
     3721 ttcaacaatg actgcttcgg ctattgccgt ttgctctgat tcatcagaag tatcgtcaaa
     3781 tccaattaga taagcaatga taaaggttgc aacaaaggcg attacacacg taataattgc
     3841 gtgaacaata ttcattggat tttctccaat aaaggctggt aaggctgcta agccagggga
     3901 aacaaaggca tatctgacta aaccgctcaa gccagcatac aaaccagcaa ttgcaccacc
     3961 aatcatggaa gcaactaatg gttttttcag cttcaaggtt actccataga gtgaaggctc
     4021 agtaattccc attaaagcag tgatcccagt ggacacagct agctgcttca actctttgtt
     4081 tttagttttc gcggctacag caaaggtagc acctgattga gcaatattag aagccagcat
     4141 tcctggccca ttgatcattt cagaacccgt actgctcaat tgcatagtag cgattggcgt
     4201 cattgcccaa gctgtacccg taaccaccaa aaatggctgc aaaccaccca tcaaaaatgg
     4261 aattaaccaa gctgctttgc cgttaatcca ttcagccccc attgctataa gatcgttcag
     4321 caacgttcca aacggtccaa tgacaaccaa cgctaaaggc cccataatta aaaataccag
     4381 catcggctta gtaaagaatt taataaacga aggcgaaatt cgatccgcaa agggctctac
     4441 ataggacatg atccaaacag taaagataat cggtaaaact gatcccgcat aatctgcagc
     4501 taataccgga attcctagaa aagaaacggc atcgccagta gaaaaaagct gtgaaatatt
     4561 tggatgaagc aaaatgccag caacagtaat cgccaaaatt gggttgacat caaactttat
     4621 ggctgcccca taggctaata gtactggcat aaaataaaag gctgcatcag caatagtatt
     4681 caaaatgatg taagtttgac tactgtctga tagtacacca gtcaaagtca agacggccaa
     4741 aatggctttt accatcccgg cacctgttat agctggaatc atcggtgtaa aggtggtaga
     4801 aatgacacta atcaatcttg aaataatact ttcttgattg tctgaagagg ggcccttttt
     4861 aattgaagtt cccacttcct tttgaatttc cttgaaggct gtttggactt cattgccaat
     4921 caccacttgg tattgtccac cgctatccac cacactaatt acaccaagtg ttttttctaa
     4981 ggctttcgta tcggctttcc ttttatcttt gagttcaaaa cgcagtctgg tggcacaatg
     5041 ggttaagctg accacatttt cttttccccc aacctgttgc aatacatttc ttcctacttc
     5101 tctgtaatcc attattctct ctccttaaag cagtaaaaaa gaccaagtca aaccacgccc
     5161 caatcaacat tgaagtgtta gtttgtcttg gtcttgcctg cataatcagt aacatgccaa
     5221 taacgttatt ttgctgtaat ccaataatag cacattatga tagcgcaatc aacagggaat
     5281 ttaatttttt atcttttcgt ttgaccgtat agggtaatat aacagttaga tcacatcaac
     5341 tagggtatat ctgcgtatac ttactgctaa aataagtata tctaatatta actataccag
     5401 taaaaccatt cctacataac cagcgggaaa ttcccaaaat cgtgaaagca tcaaaaatgc
     5461 atataacgga tcacacaact tcaacaattc tgctcttggg aaaaattgat gaatttggtg
     5521 agtaaaggat ggaataaaca gcattccaac aatataggca acaatagtga tacttgtttt
     5581 tttggtaaaa atcagtaagc aatagccaat ttcaatcatt ccgagatata aaggtatcaa
     5641 tagaatgatt tgggctagta ttcttaaaaa ttaactagca aatgtttaaa ctgatttatt
     5701 tctaatctac atgtagtaag tatttcaatt ccaccaatta tcaataaaaa taccaaacat
     5761 atccata
//
LOCUS       DAMYXM010000110         5757 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.114, whole genome shotgun sequence.
ACCESSION   DAMYXM010000110 DAMYXM010000000
VERSION     DAMYXM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.114"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..972
                     /locus_tag="PNS21_002645"
     CDS             <1..972
                     /locus_tag="PNS21_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDI5160281.1"
                     /translation="IKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGV
                     FWNYSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPE
                     LFIIFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQG
                     LLEYASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFY
                     KSFGNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIM
                     SVFFFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            969..1688
                     /locus_tag="PNS21_002646"
     CDS             969..1688
                     /locus_tag="PNS21_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDI5160282.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            1703..2788
                     /locus_tag="PNS21_002647"
     CDS             1703..2788
                     /locus_tag="PNS21_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDI5160283.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            2924..4456
                     /locus_tag="PNS21_002648"
     CDS             2924..4456
                     /locus_tag="PNS21_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDI5160284.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            4634..4855
                     /locus_tag="PNS21_002649"
     CDS             4634..4855
                     /locus_tag="PNS21_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HDI5160285.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
     gene            5437..>5757
                     /locus_tag="PNS21_002650"
     CDS             5437..>5757
                     /locus_tag="PNS21_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDI5160286.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTT"
BASE COUNT         2010 a          605 c         1101 g         2041 t
ORIGIN      
        1 attaaagatt actataaagt attcgctata attgaaaaat tattctggat ttcactgcca
       61 ctttgtttaa ttgaaagatt catggtcacc tatccaactg gaacaattgt aggagatatg
      121 ataggtggag ttttttggaa ttattcaggt tgtaatatgc cattgaattt aattttgtgt
      181 gtgacagtta ctgttgttac aatcggatat ttcaagggag aaaaaaattt catccatttt
      241 attttatgtg tttgtgcagc actttttatg tctgcattag ctgaattgaa agtttttatt
      301 cctgaattat ttataatatt tgttgcaata gtattcactt taaaagttga ttttaaagta
      361 gtgatcaaga tattatcatg cgtaatactt gtgtcgatag tattatcctt ttttataaca
      421 atatttatta cattgaacga taatggacaa agctatgctg ataatttcac aattcaaggt
      481 cttttagaat atgcgtcaag agattctgga tatgatggtg ttggtgattt aaatcgaata
      541 actggtatct ctagagtagt agatgaaata tttaataata attttagatt agtcctaatg
      601 ggagtaggca ttggcaatgc agagtttacc aatttttttg tcagtgagtt ttataaatcg
      661 tttggtaatc ttcattatca gtattttcat catatttggt tatttataga agttggaata
      721 ttaggagttt cgatattcct attttttata ataaagacat ttttcaatgc aaaaagatct
      781 tttatgaagg ataaagatgc aagtcttgtt aaagttattg caattatgtc ggtattcttt
      841 tttgtgtata acacaacact taggggtgaa acaacggggc cactgattta tatgatttta
      901 gccatgcctg aagttgtgaa aaggaaagag cattattata gtgaaaaaaa tacaaaagga
      961 gagatattat gattcctaag aagatacatt actgttggtt cgggggaaat ccacttccga
     1021 aaaaagatag aaagtgcata gaaagctgga aaaagtattg ccctgattat gagattattg
     1081 aatggaatga atcaaattat gatttaaata agaaccgtta tatggccgat gcatataaag
     1141 aacgtaaatg gggttttgtt ccagattatg caagatttga tattatttat aatcatggtg
     1201 gattttattt agatacagat gttgagctaa ttaagtcgtt agatatactt cgtgaaaatc
     1261 atgcctatat gggatttgaa ggggaaatct gggttaatgg cggtattggt tttggtgcag
     1321 aagaaggaca ttctgttatt aaaggtttaa gggatattta cgattcatta agtttttatc
     1381 aaaatgatgg tacattgaac ttgaaaccga gtccgtatta tattacagat tatcttgaat
     1441 ctattggatt ggttcgcgat aacagcgaac aaataataaa tggcctgcat atctatccta
     1501 cggattattt tgctgccaag gattatgata ctggaaaaat ttataaaact agtaatacaa
     1561 taagcattca tcaatataat gcatcttgga aaacacctaa acaaaaattt gtttatagat
     1621 taaaatcaac aattggacaa aaaaaattta acaggttact tgcactgaaa aaatttttac
     1681 acttataaag gagaaaagta ctttgaagaa aatttgtttc attagtggtg taatttctcg
     1741 ttctggggga actgaaagag tagggagtat tattgccaat agcttgtctg atttaggtta
     1801 tgatgtcact attttaagct tttggaatag tggagagcct tattttgggt tagataagac
     1861 gattaaggtt gattatatgt taaatcctaa aacggaggga aaattatata gaacttattt
     1921 atatccggtt tggaaattgc acaagtatat atgcaaaaat gaatttgata tagtaattga
     1981 tattgatact ttgttgtctt tgtatactac aagggcaatt aaaggaacaa agtgcaaatt
     2041 gatttcttgg gaacatttta attactggac aatggaaatg ctgaaagaaa agaagagatt
     2101 caaggctaaa aaattgataa aaaaatatgc aaagaggctt gtggttctca cagaagaaga
     2161 tcgtcaaaaa catattgatg aatatagttt atcagaagat tttgttataa ctatgccaaa
     2221 tccgtgtata tctaatatcg agtacgaata tcattatgat aaaaagagat ttcttgcagt
     2281 agggcgatta gtggaagaga aaaattttgc tgacatgata agtgcttgga aaattgtaga
     2341 aaaaaaagta gatgattggg atcttatcat tgtaggaaaa ggtgaattag aaaaagatct
     2401 tcgccagcag ataaaagatt taaatctgca aagagtaact atagcagggc attcagacca
     2461 tgtggaggaa tattataaat ctgcatcttg ttatgtgctt agttctgttt atgaaggatt
     2521 tcctatggtt atattagaag cacagtctta tggattacca gttatttctt atgattgtaa
     2581 aactggtccg agagatttgg tacatcataa ttttaatggg atgttggttg aagataagaa
     2641 tatagaccaa ttagctaaga gcatgataat gtttactaag aatactgatt tagcaatgag
     2701 aatgtcactg aacgcatata ataatgtaca aaaatttaat ttgaaagaaa ttacaaaaca
     2761 atgggttgct ttgattgaaa atgtatagtt ttttgtggtt gtccaaacat gaaaattagc
     2821 gtaattaatc aaagagctag aagaaaccta ttacatttca agattttaga aattatatag
     2881 tattaggtaa caataaaaaa tagaaagttt ggagccatat gatatgataa ttaatcgtaa
     2941 acaaaatgct aaaaggaata taattactgg aacaattttt aagttatatc agattatcat
     3001 tccctttttt atgcgtacac ttatgattta tcagatggga atggagtatg ctggattgaa
     3061 caacctgttc acatctatat ttcaaatgtt aaatttagcg gaattgggaa taggtgctgc
     3121 actaactgta agtatgtata aacctatagc agaagacaat agaaaagaga tttgtgcttt
     3181 actgaacgca tataaaaagt acttttcaat tattggatgg atagtgttta cattaggatt
     3241 gttatgcatt ccttttttga gatttttagt taaaggttcg attcctggaa atttaaatat
     3301 gacagttttg tatttaatgt atttgataaa tactgttttg tcgtattggt tattttcata
     3361 tagaaagagt ctaatatatg cacatcaaag aaatgatgta attaacgtgt ctatgctaat
     3421 tacttttaca ttccaatttg taatgcaggc atatgttctg attttcttaa aaaattacta
     3481 tttgtattta ttggcttcta ttttgggaca aatagttctt aacttaatgt gtgctgttgt
     3541 ttctcaaaag atgtatcctg attatactcc tgttggtgag ttagagaaag aaactattga
     3601 cgatattata aaaaaagtaa aaggattagt tacaaacaag gtaggcggta caatattacg
     3661 atcttcagat tcaattatta tttcatcttt cttaggtctt tcagtacttg cgatttatca
     3721 gaattattac tttattattt ctgccataat gagtatattt gcaataattt atgaatcatg
     3781 tatagcaggt ataggtaaca gtcttattgt agaaagtcaa gaaaaaaatt atatagattt
     3841 ttgtgatatt acaacaatta taggttggtt tctggcaatt agttgtgcat gctttattac
     3901 attatttcag ccgttcatga ttatctgggt gggaagtgat aatcttatga gtatttcatt
     3961 tgttatattt atgatagttc attttttctt atatgaaatt gatcagttaa ttgggttata
     4021 caaagatgct gctggaatat ggtacgcaga caggtttaga ccattaatta cagcaatgat
     4081 aaacattgta ttaaatatca tcctcgttaa ttggttcgga ttatatggag ttttggcggc
     4141 aaccattgtt tcagtattgt tggttgattt accctggtta ttatataatg ttttccaaaa
     4201 tatatttaaa tttgaatcgg caggtaaata tgtttggaag ctcataagtt tgtgtgtaat
     4261 agcttttgtt gcttcttatt taagttattt tgcttgttcg tatgttaata tcggaaattt
     4321 caaagtgata agtttagtga taaatttagt tatatcggtt gtcatatcga ttttagttat
     4381 tcttctgttt aatatccgta ataagaagtt taagtatatt ttgaaagttc ttacgtcggt
     4441 attggtaaaa agatgagaag agacatcaga atgatatata aagtaactac aatttttagg
     4501 ctgccgaata ggcgaacgat gtatagtttt tgttccaacg aagactcttg ttgaaattgg
     4561 agatgatatg aaggagttac aattttgact catgtttatg attgatcggt tttaaaagta
     4621 gtatggagaa gctttgggat catcggagag gatcaaaata ggtaataatg tttttatagg
     4681 aatgaagtct agaatactaa agggtgtaca tataggaaat aatgtagtta taggtgcaaa
     4741 tagtttagta aacaaggacg tatcagacga ttgtgttgtt gcaggaaatc tagcaaaagt
     4801 aattatgtcc ctagatgaat attataaaaa gataaacaat atgaagaatc cgtaaaactt
     4861 gtgaaactat accgagaata atatgggaaa aatattgatg aaaaagcatt atatgagttt
     4921 ttagggctgt ttacagatgg agatgacaat ttgtcgaaga gttgggagca atgcaaaatt
     4981 tagtagggga ttataaacag actgacaagg tatttaaaga acatgcacaa gtatttcata
     5041 atattgaaga atttttgaat actgtgagat aggagttgct acattgagaa aagtatgtat
     5101 ttttccaatg taaaagatta cgagtttgaa aacgacacca taatattatt ttttaagaag
     5161 atattataga gcttaggaac ggaagtttat ttaaggatgt tgaataaaat gaaaaataag
     5221 attaggtgga tgaaaaaggc agatgatttt ttactataat taaaaatatt gttactatgt
     5281 tatcaatcgt ataaaaatac ttactcgtta tcaagacgat tatttcgaat attttaggaa
     5341 aaataaattg aatattagag aatgtgataa tacactctaa tatgatcatg tgtaattgga
     5401 agcgataagt gtatacttga tattggagaa ataattatga taatagtgat tgcagaaatt
     5461 attacagata tattatataa attatatgaa ccctttgggt ttgctgtttt attgacaatt
     5521 ttaggactgg tctattttat gtatgcaaca gatggcattg ctgcaggtga gggctataaa
     5581 cgtatactaa tggaacttta tcttcattta aagaaggata gaaaatatag aaaagtttgt
     5641 gctttgatat tctatactgc gatgatttta tttacaacat taatcaatcg aacaatttgg
     5701 ttaaatcctt tatctgatgt tatgggagac tggtggattt ataagtataa tacaaca
//
LOCUS       DAMYXM010000111         5575 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.115, whole genome shotgun sequence.
ACCESSION   DAMYXM010000111 DAMYXM010000000
VERSION     DAMYXM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.115"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            649..981
                     /locus_tag="PNS21_002651"
     CDS             649..981
                     /locus_tag="PNS21_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDI5160287.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            1600..1941
                     /locus_tag="PNS21_002652"
     CDS             1600..1941
                     /locus_tag="PNS21_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HDI5160288.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYYQKLTDNPIGFGLLPEDDEGNEWFKMTLTNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            2055..2294
                     /locus_tag="PNS21_002653"
     CDS             2055..2294
                     /locus_tag="PNS21_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160289.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            2406..2600
                     /locus_tag="PNS21_002654"
     CDS             2406..2600
                     /locus_tag="PNS21_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160290.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFE
                     QLRRTYVKTSSNCQLLLEVS"
     gene            2686..3357
                     /locus_tag="PNS21_002655"
     CDS             2686..3357
                     /locus_tag="PNS21_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDI5160291.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQQEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            3410..5386
                     /locus_tag="PNS21_002656"
     CDS             3410..5386
                     /locus_tag="PNS21_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDI5160292.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            5399..>5575
                     /locus_tag="PNS21_002657"
     CDS             5399..>5575
                     /locus_tag="PNS21_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDI5160293.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETD"
BASE COUNT         2007 a          825 c         1196 g         1547 t
ORIGIN      
        1 aaaaagagaa caaacgtttg catgttgctg ttttttagtt ataataatac gcgaagagag
       61 gtaatttcat tttttaaaaa agctaaaaac cttgatatta cttcttttca ggcaatacga
      121 atattattcg cttacatttt tttgtataaa tttagttgtt ttaaatttaa ttgtctgcta
      181 cgcttgtgat gaattaattt tcgttctgca cttttggacg ataaatttgg acaattgaaa
      241 atacaaaaaa aagaaaggcc ctgtgagact cggaaggctg gtaaccttcg tcgacgtcca
      301 caggtggcat taagacaacc tttaatccct taatgcgttt acctttcagt gttacggtat
      361 tacccgcaac ggatttagta taacagatat tcaaaagtga taccactttt tttggctgtg
      421 ttaaggttaa aaatacgtga tattacgatg aatctatgcc taagcataat gttataaaaa
      481 tgtccgtgtg gctctttgcc gtatgacgaa cgctgaaatt agagggaacg catgtttgga
      541 tgcgttccct cttttttgtt tgtcatgaac gaaaatcaat tctctatttt tgaaaaataa
      601 caagaaaaac ttattaaaaa tatttaaagt aagtgggtgg attattttat gtcatataca
      661 ggaattttga gtctcgaaga tatttgtcac tacggtaaac ggtgtaccgc tacagagaaa
      721 atcacgaaga aactatcaac tggccaaagt aaaaccgttg tgcagtgtaa gaaatatata
      781 atccagaaag acaaagtctc agaggaaatg atttattacg ctggtaaaca aaagcaaata
      841 attttaaaag atcctatccc tttaaaagaa ctatatccaa caatcaaaca tgtttatgac
      901 caaaacggtg tactgattgg tcgaagaaaa aatggagtct tgcgttgtac cgcaaaaggg
      961 atggggcgtt taatcagctg aattgatcat tctcataact tgtattttat cgtttttcgc
     1021 ttgcttgaaa atattgtcga gacttctaaa agaacttaga attactagaa aaataaggag
     1081 tagacttgat ctatcaacaa ttcctttcgg ggggtgacgg ggtaggtttt tacgattggt
     1141 aacgtaaaaa attggtcaat cgagtaaatg cccttgtttg gatagtggtg aaatgaattt
     1201 gtgtaaataa tatgcacaga gtaaacaaga gggaatttag aagttagaca agttagaata
     1261 gcgcctagat taatcatgag cagctactaa gggatctagg ctttgacgca aactcatgga
     1321 ataaggagta ggtcttctcg taatggggag gacgcacaag aaattgtgtc gtgtgctgca
     1381 gtacatgtag atacttgcga ggacgttggt cattggatca atcgagcatt taccgatttc
     1441 tactagggtt tctcgtttcg gacgccataa acgagtcgat attttttatg tatttttaag
     1501 taaacgaaaa acgataaaat atgaacgagt cctgaataaa gtgaaaaaat tgatataaaa
     1561 catagagaaa atgaaattta cgtaatggag gaatgacaaa tgaaaaatta tgatccgaat
     1621 atacgattgg gcacacatac tattaaggtt tctttccaac gttgggatta caaaggattt
     1681 gtgacgttta gaagaggcgg aaattgtaaa ggattagatg ttttagcatt agacgaggat
     1741 gatttatact atcaaaaatt gactgataat ccaattggat tcggcttgct acctgaagat
     1801 gatgaaggta atgaatggtt taaaatgacg cttacgaatg ataatggaga cgaattatct
     1861 gttgaagata cttggtcata tttgagtgat tatattgtca gcgttgaaat cattgaattt
     1921 gtagcggata aagaagaata aaaactggag gaataaattt tacagataaa ggcttagaag
     1981 aagtcacgac gaataataaa aaggtagtat taccttaggc attgagtaag gaattcattt
     2041 aggaggaaaa ttagatggcg cgaccagttt tagaggcgat ggatatggat actttgacaa
     2101 aaggaaggta tgtacagctg ttagctcgtg gttattcacc taaagatgta ttcaaatccc
     2161 tgtctaaagg aacagatatg gaaaaagaaa gattacgaaa agaattcgat tattggagaa
     2221 cccataatgg aatcaaggat ttgaaaccag cacggccagt aattaaacga aagaaaaaga
     2281 taacccaaga ctgaagtgta gagcgtatgt gatcataggc ttttttattt ttgcaaaaaa
     2341 caaggaaggt aggaaaactc atgaattatg aagaaatcga aaacagaaaa aaagtgtcga
     2401 aataaatgga agaaaagttg ctaaaaacga tgaaacaaaa acaccttaaa agattatctg
     2461 tcatgcaata tatcaatgat atgcagataa caggaaaaga gaaagcttgt ttgttaggtt
     2521 caatgaaaaa ctttgaacag ttgagacgaa cttatgtgaa aacaagttct aattgtcaac
     2581 tattgttaga agtctcatga cattcattaa tagaattcat ttttaaaact ttttctatgg
     2641 aaatgagaaa aagatttttt gttttgtctg aaagggggta gctggatgag aaaatggtta
     2701 tttggttttt taggagtcgc tttaatcgtc ttttgcagtg tgtttggcta tgttagctac
     2761 caaaaacatg aaggagaggt gtttaagcaa aatatagaaa aaaagatgcc cgtagatcaa
     2821 atcaatgcac atgcaaagag ttataaggag gatgcaacga acgtcaataa tgatatgtct
     2881 ttggggcaaa tgttgtccat acaacaagaa gcaatcgaaa tgggcgtcaa taaacaagta
     2941 tttgcacaaa ttcagattcc tgcacttggt ttagcattgc caatttttaa aggggcaaat
     3001 caatacactt tgagtcttgg agcggctact tatttttatg aagatgctga aatgggcaaa
     3061 ggaaactatg tcctagcagg tcataacatg gaaatgccag gtgttttatt ttctgatatt
     3121 caaaagttgt cattagggga ggtgatggac ttagttagta acgatggcgt ctatcgttac
     3181 aaggtaacgc gtaaatttat cgtacctgag tactttaaat taatcgatgg cgttcctgaa
     3241 gagaactctt tcttgtcttt gccaaagaaa ggagagaaac ccttattaac gttgtttact
     3301 tgtgtttata cgtcacaagg aaaagaaaga tacgtggtac agggagaact acaataacaa
     3361 ggttttaatc aacgtgagtc gttgaaatat gaaatgagga gagtaaaaaa tggcaataaa
     3421 tcggtatgta aaaaaagctg gaatgcttat tggaatgtta ggaatcattg caggctgctt
     3481 tcagggatat cgagcatatg cagaagatgt cacgcaaaaa acgccaccag agaaggtcaa
     3541 tatcacggtt cataaattaa tgtacgatca aggaacgcaa ttgaatgttg atattgacgg
     3601 gatcaaaaac gatggttata cgcacgatca atatcctaca ggtgtaacga aatacaacaa
     3661 ggcagattat ggtgatgttg agtttacgtt aggaaatatt acggatcaag tattaccgac
     3721 tgaagattct gatctaacca acgcgaaggt ggacgaaatc gttaaagatg tagaagacaa
     3781 gggatctaac agtgagtacg tcaagaatgc aaccaacaaa attacttcag ctgtagatga
     3841 aaatggtgag atcacttttg ctgatcaacc agcctatgtc aattccaaag ggaatgtata
     3901 tgtggtttat gaaagtaaat ccgctgctgg attagtcaca caaaaagcaa aaccaatggt
     3961 tgtgattgcg ccaatgactg ataatacagg ttcaggtttt ttaaaagaca ttcacttata
     4021 tcctaaaaat attgtttcta agttgagttt tgaattaacg aaatttggtg acgatggcac
     4081 agctcaaagc aaacaaacac ctttaaaagg ggcaaaattt gagttataca agggcgaacc
     4141 tggaaaagga acaaaattag gagatcttgt atcggatgat caaggaaagc tgactgctac
     4201 tgatttgacg ttaggaaaat actattttgt ggaagttcct tcagaagttg tagtgggatc
     4261 agataaagaa ccaacagccg atcaatattt attaggcgca gacgctcgca acgatgcgca
     4321 caataaatta acctttgaaa ttacgaatga tggcgtgaca agtgacttaa aagcttcata
     4381 cgtaaactat aaagcaccag tgatcgataa aacagtaaca aatggaacag gacaagaaca
     4441 ttctttccaa attggtgacg cagtaaatta tcaaggaacg attcatatcc caacagacat
     4501 tgcaggcgga gcagacggta tcacagtaaa cggagtgaaa agcgaaactt ctccttacag
     4561 tgtatttaaa tggggggata cagcaggaca aggactttct tatgtcgctg ctaaagcaaa
     4621 tatcaaagta acaaataaag atggatctgt ggtattaaaa gaaaatacag attataaaat
     4681 ccaaaattca gaaaatggct ttgtgatcga ttttatcgtg aataatggcc aagtaagtga
     4741 tactgtagca agtttacacg gacaagatct tcaaatgact tataacatgt atgtaaatga
     4801 cagcgcagct gttgccaatc cattaacaaa tagcgtggat ttcgtttata acaacaatcc
     4861 atttaatcag gaagaacacc acgaaaaaac gaaagctgac gttgtgactt atggtgcaaa
     4921 attcttaaaa gtcgatagcg gactatttgg aacaggaatc aaagcaacac cgcttgaagg
     4981 cgcagaattt gctgctaaaa acgcagaagg aaaatattat ggtggattag ttgatacgga
     5041 taaagacggt gtcaaagaag cagtatgggt agacgatgta gcaaatgcag cgatcctaaa
     5101 atcagataaa gaaggtcact ttgaaatcac tggtttaact gaaggtgaat attcactgga
     5161 agaaacaaaa gcgcctgaaa actatcaaaa attaacgaaa gagatctctt tcaaagtaga
     5221 caaagattca tacaaagaag aaaaccgaat cacgatcaaa aacaatcaaa aagcttccgt
     5281 accaatgacc ggtagcaacg gtttccaatc ttacgtattg atctcttgct tgttgcttgg
     5341 ggcaggcgca atttctgcag ttgtttactt taagaagaaa gcctagaagg atcagctaat
     5401 gattatcaga caccctaaga aaaagcgaat catgggtaaa tggatcattg ctttctggtt
     5461 actttcagct gttggtgtct tacttttgat gcctgctgaa gcgagcgtgg cgaaatacca
     5521 acaaaatcag cagattgccg caatcgatcg aacaggaacg gcagcagaaa cggat
//
LOCUS       DAMYXM010000112         5431 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.116, whole genome shotgun sequence.
ACCESSION   DAMYXM010000112 DAMYXM010000000
VERSION     DAMYXM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.116"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(12..482)
                     /locus_tag="PNS21_002658"
     CDS             complement(12..482)
                     /locus_tag="PNS21_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDI5160294.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="PNS21_002659"
     CDS             complement(641..1099)
                     /locus_tag="PNS21_002659"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDI5160295.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="PNS21_002660"
     CDS             complement(1270..2415)
                     /locus_tag="PNS21_002660"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDI5160296.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="PNS21_002661"
     CDS             complement(2428..3345)
                     /locus_tag="PNS21_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDI5160297.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="PNS21_002662"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="PNS21_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDI5160298.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            complement(4476..>5431)
                     /locus_tag="PNS21_002663"
     CDS             complement(4476..>5431)
                     /locus_tag="PNS21_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDI5160299.1"
                     /translation="GTLELKVPRTRDGHFSPTVFERYQRNEKALIASMLEMYVSGVST
                     RKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPYLMTDVLYIKVRE
                     ENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWITFFEYLKERGLQGTELVISD
                     AHKGLVSAIRKSFTNASWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTDINLAR
                     EAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYIKFAK"
BASE COUNT         1636 a         1010 c         1031 g         1754 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtagtcaa gtccacaatc
     4441 ctgtgtaaaa tactatacaa tgctctcact attttttact tggcaaattt aatatatttt
     4501 cttgatgaag aaagccactc atcgtgttga tcaataagga ctgctccgat taaacgattg
     4561 gctgaaacgc agtttggaaa gatacgaata attttttctc ttctgcgcac ttcttgattc
     4621 agtcgttcaa ttagattggt actctttagt cgattgtggg aatttccttg tacggtatat
     4681 tgaaaggcgt cttcgaatcc atcatccaat gatgcgcaag cttttgaata ttttggttga
     4741 tcgatataat catgaatcaa tcgattttta gcctcacgag ctaagttaat atctgtgaac
     4801 ttaaaaattc ctttaacagc ttctctgaaa gattttgaat tttttttagg aatggtggta
     4861 aagatatttc ttaggaagtg aacttggcat ctttgccaac ttgcgttggt gaaggatttt
     4921 ctaatggcag agactaatcc tttgtgcgca tcagaaataa cgagttctgt accttgtaaa
     4981 ccgcgttctt ttaggtattc aaaaaatgtt atccaggtct cttcgctttc gccactttga
     5041 atcatgaagc cgataatttc acggtcgcca tctttggtta ttccaatcgc tatatgacag
     5101 ctttttgaga gtactcgatt ttcttctcgt acttttatat agagtacatc ggtcattaag
     5161 taaggataat ttttttctga taataaacga ttctgccact cgttaaccat tggttctagc
     5221 tgttctgtta agctagaaac gaaggactta gagacggatt taccacaaag ttcttccaca
     5281 atttttgata ctttacgagt tgaaacgcct gatacataca tttccaacat tgaagcaatg
     5341 agggcttttt cgtttcgttg ataacgttca aacactgtgg gtgaaaaatg gccatcacgt
     5401 gttctgggta cttttaattc tagcgtgcct a
//
LOCUS       DAMYXM010000113         5430 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.117, whole genome shotgun sequence.
ACCESSION   DAMYXM010000113 DAMYXM010000000
VERSION     DAMYXM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.117"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(153..428)
                     /locus_tag="PNS21_002664"
     CDS             complement(153..428)
                     /locus_tag="PNS21_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HDI5160300.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(400..1353)
                     /locus_tag="PNS21_002665"
     CDS             complement(400..1353)
                     /locus_tag="PNS21_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDI5160301.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1965..3458
                     /locus_tag="PNS21_002666"
     CDS             1965..3458
                     /locus_tag="PNS21_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDI5160302.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3593..3889
                     /locus_tag="PNS21_002667"
     CDS             3593..3889
                     /locus_tag="PNS21_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDI5160303.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            3990..4841
                     /locus_tag="PNS21_002668"
     CDS             3990..4841
                     /locus_tag="PNS21_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160304.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            4862..5145
                     /locus_tag="PNS21_002669"
                     /pseudo
     CDS             4862..5145
                     /locus_tag="PNS21_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1869 a          836 c         1014 g         1711 t
ORIGIN      
        1 tatttagtat agtaaaaact agaaacaata aaatttgtga atgttgtata cccccaaaca
       61 atacacatat tttgaaggat taggattaat aaagctaaaa atatagtaag tatactaaat
      121 atatttacta tatctagtat acttactata ttttaatctt ttgatcgtct attatatata
      181 atccgttcct cgtcgtttaa agtatcaaca taggcgtcca ttgcaagttt gactatttca
      241 tacataggaa tatcttttgc ataactaaga tcccgaatgg tttttagtac gggaggatct
      301 acttgaactg gttttctttc tttcgcagtg tactttcgtt cccctaactc gacattaata
      361 tcatctttaa ttgaaatatc ttcagcacca attttcttct tatctttgtt cgttggtaat
      421 aatgccattt agtttaatct cctttccaag aggtaggata tgattggcca ttgagtcagc
      481 atatttaggt gtataatcaa atccttcaat atcaccatgt tgttcatagt attcaattct
      541 ttcttctaat tctgtaaaga tgtctgcaaa aatagcccac catcttcgat ctacataatc
      601 atttaattgg ataccattct caccatacat ttctaaccga tccgagcctt taacaatgct
      661 tttaaaaatg tttgattcac catacttatc aattgttttt tgatagtttg cgtgttgaga
      721 ggaacgtcgc actgagaaaa gaacaggcaa aattccaatg atttgaagat gagactcttc
      781 aaaattcgtg acaatcggaa ctaaaacttt attaataaaa tcttctgttc cttccattgc
      841 gtaccgtttt aattcttgca ttgcaataat ataatctgca gcagttaaga atgcacgaac
      901 cacattatct gttgatggtg ggcagtcgat aaagatataa tcgaaatttt ttctcaattc
      961 ttctaaaggt ttaatgaagg ccaatgatct cttacgacga tcaggatatt tttcggacaa
     1021 gtattcatta agttcttcag atccaggaga gccagccata atatataaat gtggacttac
     1081 ttgagtaata cctgaaaccc agtccccttt tttgattgct gcagacatgg atcgatcaaa
     1141 gctagtaatt tgatacgttg atccaatgtt ggaagtagta tttctttgtt ggtcataatc
     1201 aattagcaac acgtcttttt caaaaagttg actagaaaca atcgctaata aatcattgtc
     1261 tgtagtctta ccaatgccgc cttttctacc tgtaagcatt acaacttttc ctagtttatc
     1321 tgatgtttta aatgcagtcc gtaagttttc catagttccc tccaatttcc tatactaaat
     1381 atatttagta tacttcatat acttaatata ttcgatatat ctaatatact ccaaattgat
     1441 aagaattcag actttataag gaatatagtg gatatactaa atatatttgc tatattcagt
     1501 atatcagata tattcaaaat gtcaaaatat atttgtgcgt tttgcgttga caatttagag
     1561 tgaaaagagg taaacttaat tctatagaaa ccacagataa ttcactattt acaaagtagc
     1621 aaaagcagtg gtactcttta aacagaaaaa ttacataaaa aaaagagacg gaaataattg
     1681 attttagcag aatcaattat ttctgaatca catggttagg acatgtgttc acgctcgtct
     1741 caagaatatg taagtagcta ccaactactt atattaaaag ttgatcggat accttttccg
     1801 attgccttta ttctaccaaa aaaaggcgag caactcaaca gaagaaagtt tatttagtgt
     1861 acttaaatta aatattctta aacactgaag agagaacaca tactatggct gagattaaag
     1921 ctaaactgtg ttctcttttt gtttgagaaa ggacgttaca atcgatgttg agcgaaattt
     1981 attcagctat tcttaaaaac ggcttacgta catacaaata tcgaaacagc accatgaaac
     2041 ctgcaggtca tattgattcc agtaggagtg gtgctatctt tgcgtaccgt agcaagcaat
     2101 taatgaatgt tgggcgtggt atggtcatta cttcggaaga agctatttta gagaacgaaa
     2161 agaaactaac gcattggaca cctaatacct atcgctttgg tacttatgcg gatgattatc
     2221 gaacagtggt taaaggtcat tctgagaaaa acttaagtca aatcaacaca tttgtagttg
     2281 atatcgattc aaaagagaac catcaaggcg aaattatttt agcctgttta gatcaagtgg
     2341 gctacatgcc aacgctaatt ttagaatcag atcatggtta tcaagtttat tttgtactaa
     2401 agacaccagg ctatgtcaca aagaaaagca atttcaaggt gatcgatgta gcaaaaaaaa
     2461 tttcaaaaac aattcgtcta cagttggctg aaaaattgtc cggagtggat ctaggatgta
     2521 accacttcgg aattgctcga ttcccacaag aaaataatat tgtttttaaa gaaatgaact
     2581 atcagtattc ttttgctgac tggattcaat ggtcgatgaa gcttgaatca gatcaaaaaa
     2641 caaaagaaga tcgagaacga aaactaattg tctttcctga gaaaaaagaa attcgtcaag
     2701 tagatgaacc gtggttcgat atgttacttc ataaagcaaa catcgtcggc ggagaaggac
     2761 gtcttggtcg aaataatgtg attttcacgc tttctttagc ttattactct tcaggttatg
     2821 cattggatac ttgcgaatac aatatgttca cattcaacga acggttacag gaaccgctat
     2881 ctgaagcaga attagtaaag attgtacgta gtgcttattc tggtaattat caagcagcaa
     2941 gcagagaatt cattattgaa ttgtgccagc aatgggtttc accaacctta aaaaataatg
     3001 atctgttcat tcaacgacgt ggctggtgga aatttaaaaa gccacgagat caaagaaaat
     3061 atagtcataa aaatgaatgg aaagaagatc tattggctta tctaagcgaa aaaagtcttg
     3121 ctagtgagcc ttatttgaac ctgtcaaaaa aagaattggc ttcacaacta acgatgccca
     3181 agcgaacact tgataagatc ttaaatgagt tacgagagga gaataaagtc ttctacaagg
     3241 taacacgagg aagaaatggc ggaatgatca ttgcttcagt caaaacgttg ttcgcgaggg
     3301 ttattcaact aagtaaagaa aagagactag attattcaac aaggatcaca gaggtatttg
     3361 gactcaacga gagcttagta gaagagacat ttagacaatt atcaaccccc ttgaaccctc
     3421 ttaaagaagt gaatcttttt gaagtagata caggttgaaa aataaaaggt gcaaattgcc
     3481 ctatacaata tatctatgat acttctgtag gtgttgctcc ctttgcctag ctacgtgtag
     3541 gtggtatact tataactgcg tatgcagtct tatgtatcat ggaggaagtt gtatgtcgtt
     3601 aaaaaattat gtctattcac atcctgttaa tgtgtttatc attggtcgtt tgggattatc
     3661 tgtggaagtg ttctgtgagc tttatggttt taagcagggg actttgtctt cttgggttac
     3721 cagggaaaaa acagttgctt ctttgccgat tgagtttctt catgggttgt ccttagcttc
     3781 tggggagact atggatcagg tatatgattg tttgtgtgtg ttagagcagg agtatattga
     3841 gtataaaata gctaacgagt taagaaaaag gaagaagtat atccagtagt agtttttatt
     3901 tacttaaaaa attggttcat tgagcaacct atcgctgcta aagtagcaaa ggaaaacgtt
     3961 aaaggaaaga atttagggaa gaaatcgaaa tgaaatcgga cgactggatt gaacacacct
     4021 ggcttcctct gaaaagaaat agcaaacgaa agaaattttg ggtattgcaa ttatggccat
     4081 taggaaaact ttgtagttac ggggcacgac ttattcttga tttaaccgtt tactcgattg
     4141 gagtatttgg aacggcttat tacttgataa atgatcccaa taggattgca gcatcagaca
     4201 tttttacagt gctaatgacg ttgatttttg tcatgctatt gtctattttt actcaattag
     4261 tcatgcaatt taccaggcaa aaattaacag caggaaaact tttcaaaagc ctattcgatt
     4321 taattgttgt aaatgcaatt ctagcgactt taataaaaca aattgaacaa tctggaagaa
     4381 caacaaaagg agtgaaggat tttttattga cgtttattca ctttgtaaat ggacacatgt
     4441 atttgtttga agtgggtatt attctctttt ttgtcgggta tgtatccacg aaacttatta
     4501 aacgttcaat tgaaaaagaa attgaaattg acctgattcc gacacttctt ggttatgagt
     4561 acaatgttga ttttggaaga tatgacgaat tgatcaatac ggatatcgtt cacacgagta
     4621 tccatgaatc tactgttaag ggcatcagat ctattttaaa aaatggccag gaaatgaaag
     4681 caatagatcg aagaaaagag atctacaatt tttatgaagt cgaagcaagc ctacgatatg
     4741 gcatcggaaa attgtggtta gatggaagta atattcgtca aaacaaacca tacatttttt
     4801 ggaacagtag tgatttagaa cttccaatga aagataaata attatggaga atcgtttgaa
     4861 aatgattcct ctatacagga ttgtgcttat agttggcatg gagttaaaaa ccttaaagaa
     4921 ggagcagtta ttcaaagtga aagaacgtca taatcagtta ctatgtagta aaacgggtac
     4981 gatgaacatt gaataccgaa cagaatattt agaattaacg aatcaaactg attttttgtt
     5041 gattcgaaag caacaagagc aagaaaaaaa ttaatggcta cttatagcca attgattgat
     5101 gaatgggaac aattcaagga atctaaaatt ccagaacgtc tctaaaagcc tttatttgta
     5161 aagagcataa ggaaacgtgg gaacactttt taaaaaaagt gttccaatag gctgtgccta
     5221 ttacgtttct caaaaataaa tgtgggaaca aaatatttaa aaataggata gtgaaaggat
     5281 agtgaaggat agtaaaagga gggtagtagg atagcaaaaa ggataggctc cttttcgcta
     5341 tcctttttaa attggaggaa ttaatatgga agtaagaata agaaatgtgg atgagaaaat
     5401 cgtaagagaa attgatcgaa gatgtgacga
//
LOCUS       DAMYXM010000114         5383 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.118, whole genome shotgun sequence.
ACCESSION   DAMYXM010000114 DAMYXM010000000
VERSION     DAMYXM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.118"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            63..2390
                     /locus_tag="PNS21_002670"
     CDS             63..2390
                     /locus_tag="PNS21_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDI5160305.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2516..2845
                     /locus_tag="PNS21_002671"
     CDS             2516..2845
                     /locus_tag="PNS21_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDI5160306.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2885..3889
                     /locus_tag="PNS21_002672"
     CDS             2885..3889
                     /locus_tag="PNS21_002672"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDI5160307.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3886..5315
                     /locus_tag="PNS21_002673"
                     /pseudo
     CDS             3886..5315
                     /locus_tag="PNS21_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
BASE COUNT         1912 a         1028 c         1064 g         1379 t
ORIGIN      
        1 taaaaattaa atttagaata acgtgtaaag gattatttga tgatttttta ttgagaggag
       61 ggatgaaaat gattaagaaa actcaaaata aaatccaacg tatatgtcaa aaacattttg
      121 tttctagact caaagctgta tctaaaagaa aatacttaca tcccattatg atttcgatca
      181 tcttgatttt tactttctta gggagcttat caagtattta tgtaaacgtt tatacagaaa
      241 cgtcaaattt tgctaaccaa acgacagagg agacaaatag tccaccctat tctgaaatac
      301 agtccataga aagcaccaat cagtctagca acaacgagac aacctcaacc acatcctcga
      361 ctcaactgat tgatccgaca gaaaaagaaa tcacagcagc aaaagcagca ggggagaaag
      421 cttattcaga aacaggtaga agccagacat ttagtactgt tgcggccact gcgaatttca
      481 acggacatac cataccagat ggatatacag tgatatatgc caaagatgga gtcattgttg
      541 cggttaacgg atcgatgagt gcggataaaa ctgcgttaca aggcgtaacc aaagcaatcg
      601 cttttattga taaccgtcca gatatccata atctgtttat tggctccaca gtaaatggta
      661 ctactatcac attcccaaaa agtatctata tgggggtggc taccgctgga aatacgatcg
      721 attcagttac acccaatgcg gataaaagtt attcttttac accctcaaca tggggagggc
      781 ttcctgccgg aggaggtatg agtacttttt caatcggtat acagttagca aatgatattc
      841 cattcacggg gtactcgatc aaaaatgggc agatcgttga tggtaaggga aatgtttcaa
      901 attttccaac ctatcaagca gcaacggttc aggtcaatgc gaatcggaca ggaacttttt
      961 cttatacagt caatggtgga gcagcaaaaa tcggaacgta cgggactaca ttatctggaa
     1021 tccatgtggg tgacaaagtg gttatcactc caacagcagt aacgggctat tcttttgcac
     1081 aaacaaacgg ttctttcaca gccctagaaa gtaacgtcga tacggtgacc tatacaggca
     1141 aacaaacacc aatcactatt aagtatcaag actattttgg acagatgatt gctccttcta
     1201 aaataatgaa tcttacttac ggaagtgctg cacaagacct gacgactaat acgcctacta
     1261 tcaacggata tacctttaca gctgtttcag cgacagaaac aaagaaccaa tcagcaatga
     1321 gtgttttagc tagtttagat acaaatggaa atgtaatcgt aaaggacgca aatggaaaac
     1381 agatttctga agtcatccta tattataaga caaatgcaac agtatcaatc aatgcaaatg
     1441 gcagtaagta ctatgatggt ctttctgttg taccgatcgt taaatatgcc tttaatgata
     1501 aaacagaatc aacatccctt ttaaacaatg atctcagtat tgatcaaatc acatggaatc
     1561 caactgattt caaagcgatg aatgaaaagg gagttgaagt aactgcgcct actgaaattg
     1621 gaacctatac gtcttggcaa ttgacaaaaa gcggactagc taaactagct gccagaacaa
     1681 actatttatt tactatcgtg cagacatctg atgtctatac gattaagcaa atcagcggtg
     1741 cggtagagct cagtggcagt aagacatatg atggaaaagc aggtgtcccg tcaatccatg
     1801 tcaagctagc agagggtgtc actagttcgc ttactccagt acaagtggca cttagcagta
     1861 tggattacac agcagatgca caatcagcaa aaaatatggt taatgctggc agctatacaa
     1921 ttaaactaac gaatagcgga atagacaaag tcaaagcagc aaatcccaag ttgtcattta
     1981 ctgatatagc caacacatca ggaacttata ccatctataa agctaacgca gtgattactg
     2041 tcgatgacgc tgcctttaat tatgatgccc aaagtcactc gatttcggtt ggaaatctcc
     2101 acgttaccgg tgttgtctta ggcgaatcat tggattatac cctaacagtt aacagccgga
     2161 cagatattgg atcacaaata gtaacgatta gtttaggcga aggaagtgta aacaataatt
     2221 atacgattat cgcaacagat accgctaagt tgacgatcaa tccgactctc ttgcttcctt
     2281 cgacaggtgg gataggtcta ctgccctttg tctttttagg actgatcttt attttttctg
     2341 gcttctttta ttttatccat cgtaagaaaa aagcaggtga acagcgatga aaaaaacaaa
     2401 aaatgtacgt gtggatatat ttgctgttct cttttttatc ctactggtct tttttatagg
     2461 ttctccgact gtgtcagcta gagaatcacc tattacattt actttgcata atcgaatgtt
     2521 cgaaagcgat aagatgccaa gtatcagtca aaacgacgga ataaatatcc cggataactt
     2581 tcaaggagta aaagggttga acggcgttac tttcgctata tatgatgtct cggatgaatt
     2641 ttacaagctg cggtcagaag gaagtagtgt ggaagacgca caaagaaaac tagcacaaaa
     2701 aagtgatagt gaaaaaatac tggcagaaaa tgtgacaaaa acagtagacg aggaagaggg
     2761 cattgcttct tttagtgcat cggacaaaga tgagcaaggt cgtgacgctg tttaccgatt
     2821 tgcggaaata aagacttcag attaagtaaa agaaaaatca gcaccatttg tcgttgtgct
     2881 tcctgtgaca gattcatcta atcataaatt gacgaccatc cacttgtatc caaagagcga
     2941 acaaaaaata cctgctgcac tcacattaac aaaaacagta gagaataagc agacagattt
     3001 tgcagatggg gataaagttc cttatctcat caccgtaaca attcctgaga acatcaacga
     3061 gataggaacc tatacgatca aagatacagc tgatcctcag ttatggctag aaccagaaac
     3121 gatcgatttt ttgattggtg gagacaagat tcatacattt cacacacaaa aaacaaaaca
     3181 tggttttgtc ttatcagatt ctggaaaaga tcttagttcg tttgctggga aaaaattaac
     3241 gatccgttac caaatgacat tgaaagaaaa cagtgaaaaa acgacatttg ataacgatgt
     3301 acgtttgacg actgacaatc aaacagtaga aacacatctt gccatccaaa caggaggcaa
     3361 acaatttgtc aaagtggatc ggcaaacgaa acaaaaacta gcagatgctc aatttctagt
     3421 caaaaaaaat gaccaatact tagcgcaaga aaacgggata aatcattggg tagccaaaga
     3481 tgattcaaga gcaacgattt ttacttccag taaagatgga acattttctg tccatggatt
     3541 gtcttttgga agttacgaac tagtggagat taaaccgcct aaaggatata tattgagtca
     3601 atcaaccatt ctctttgaag ttgaaaaagg cagttatgat ccgcttcatc ctataccatt
     3661 ggaaatcatc aacgaaccaa aagcaaatcc gacgaataat ggaaaaacgc cagttcagat
     3721 gcagacaaat ggtccatcaa atgaaaaaaa tggaggaagc tttccaaaaa caagcgaaga
     3781 gatgcttgga ggtttctcaa ttttagggtt gttgctagtc ctttcaacag ggacagcttg
     3841 gttttataaa aaacaacaaa aaggaaacaa taggagggaa ataaaatgaa aaacatcaaa
     3901 acaaaaatat ggacgttttt aggaactgct atcatgctgc ttcctttcgt tctcggccta
     3961 ggaactgcag aggtttctag gccgtatcac ctacaccaga aaatgttacg gttaatttgc
     4021 ataaattgaa attcacttca gcaccagaaa atcaaataaa caacggaacg gaattgacat
     4081 ttccgaatag cgaaccgttg aacggcgttg aattcaacgt atatgacatc actgctacgt
     4141 actatccaag taaagacaca gcagttccag cagacgctac acccttcgca agtgtaacga
     4201 cttctggtga aggattagct aacctgactc ttccaggtaa atctgatggg aaagatgctg
     4261 tatacgtctt tgtagaaaca ccaaagcccg gtgtcgaaac gtcgccaaat atcgtgttaa
     4321 gcttaccctt ttataacccg aattcatcag accctgacaa acctttagac acgattcacc
     4381 tatatgctaa aaatgtcatc aaagaaagca atatcaaaat ccagaaagct gggaactttt
     4441 caggggtaga taagttagca ggggcgcaat tccagatcaa aaattcagag aatcaattcg
     4501 tgaccggttt tgatgaaaac actggtgtcg ctctctatga tcctgctgag tcagcgttga
     4561 cattcacaac tgatactgaa ggatccatca gtgtcaatgg attaatagac ggcaattata
     4621 ctttagttga aacaaaagca ccagatggtt atcaattaac caatccacaa aaagaaacta
     4681 cttttatagt aaaaaacgga aaattgcaac aaacaaccgg tatcatccat ggcgcagatg
     4741 aagccaatct tgggaatcca aacggtgtag tcaatcggga aaatacaact acggataaca
     4801 cgattactgt agaaaatgtc caaaactacg gtgatttcca cttcatcaaa caagatgtga
     4861 acacgcaaga aaaattagcc gatgcagaat tttctattct aagagcaaaa gatccaactg
     4921 ttaaattaat gaaaaaaata gcaaatcttg cctcaggaga atacccatat gcttggtcag
     4981 atgaagtcac aggttctgac tgggaagaag tgaaattgat ttctgattca gaagggaaat
     5041 tttcaatcga agggctgaaa caaggggcat actgccttca agaaacaaaa gcaccagaag
     5101 gctacgcatt acctaagaat gaagctgcgt acactgaatt tacagtagat gatgatccaa
     5161 caacttctga tgaaacaacg atcgataacc aacctaaagg aatcttgcct tcaactggtg
     5221 gtatggggat catcgtattc atcttggtag gtactgcttt agttggagga gcagtgatct
     5281 acttcaaaaa acgccatcaa gaaacagaag cttaaactat tggaaacaaa aatcatacga
     5341 taaataaacg atagtgattc aaatggggag aaaggttgtg aca
//
LOCUS       DAMYXM010000115         5341 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.119, whole genome shotgun sequence.
ACCESSION   DAMYXM010000115 DAMYXM010000000
VERSION     DAMYXM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.119"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..132)
                     /locus_tag="PNS21_002674"
     CDS             complement(<1..132)
                     /locus_tag="PNS21_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDI5160308.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..2914
                     /locus_tag="PNS21_002675"
     CDS             542..2914
                     /locus_tag="PNS21_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160309.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2975..4435
                     /locus_tag="PNS21_002676"
     CDS             2975..4435
                     /locus_tag="PNS21_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDI5160310.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERSNGTYDFDRFYQGKGDINAQILFSYKAEQYYKGYKKHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            4446..>5341
                     /locus_tag="PNS21_002677"
     CDS             4446..>5341
                     /locus_tag="PNS21_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDI5160311.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSLLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKSQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMN"
BASE COUNT         2030 a          897 c         1134 g         1280 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaaacgga
      541 catggcagaa gaaaaagaac cactagacaa tagtcgcttg ggaaaatcca aaagaaaatt
      601 agtacgattg caaaatgaat taaacgaaca aattgagaag atgtttgaac accagagaaa
      661 gaccaacggt cagccaatga acgataagag aaatgggcat tcttggttta ggcaacaaga
      721 acgtttagaa aacaaagtgc atagtctgag agaagagatc aagcaacaag aaaaacaggt
      781 agaaaaactt gaaagacaag aagagctaaa ggaaatgggc tataacaagt acggcggcct
      841 ggatatgaca atcgaaaata ttccaagaat caaagaagaa atcgaacgct ttgaaaaagg
      901 tgaatcgact ttttcagccg ctacgatccg aaaataccaa cggaaattgg aaacattaga
      961 acagctgaaa gaaagatcag aaaaaggaaa agaaaatctt cttcctgaag tacaagcaat
     1021 tattgacagc ggacgagtga cgcaatggaa aaaaaatcca accatttatt ttttgaaaga
     1081 ctatcgaaaa gttgctttag aactgaatga aaacggggga tttgaggaat caaaaaaata
     1141 ccaagccacg tcggaagagg aaaaagcagc tgttcgtgaa ttacttgaag aggctgcgcc
     1201 aactgattct ttgaaggaga aagaaaacga tcatttctat cgagtagaat tcaacgaaaa
     1261 tgatccagaa aaaggattga aaagttatgg tggggagatc gtaacattag aattgatcga
     1321 tcaactcaaa acgttggata accaatacgc cgcagaagca ggttattata aattctattt
     1381 tgaagaagtg aagaacggag aagtagtcga tcagatccga atggatctag gggatggcgt
     1441 caacagtaat caacccatct atttagagtt agcaaaattg tgcgaacaag tacctgaaaa
     1501 ggaacaaaaa caaacggtca aaaatgaacc tgtttcgaaa gaacaaatgc agtcgaaaga
     1561 aacaaaaaca aaacaaaagg gctatcaaaa acgaacggca gaagagatta agcaagaagt
     1621 caaatccctt tcgcaacagg cactggatgc ggtgaaaaag catacccatt ctcctgaaga
     1681 cgtgaaagaa cttttgaatt ttatgtctcg ttttccagaa cgatccttta gaaatcaatt
     1741 attgattgag gaacaatttc caggtgcaac ggcttgttta ggtcgtgctg cgttgaaaaa
     1801 agaaggaatc tatatcaaaa aaggggaaca agggaacaag atctttgtca gaaaaacggt
     1861 caaaggattt tatgagaaag gacgtggctt tgttcgagaa acagaagcta caccagaaga
     1921 taaaaaacga atcgaaagtg gtcaaatcga agtgatcaaa aaaccttact acacgattga
     1981 aaaagtgttt gatatcacac aaacacagtt aaaacctgaa gactatccga aaatctttcc
     2041 gaaccgtgtc tttgattttc aattggataa aaaaggacag tcagaacttc aagctgggat
     2101 tcaagcggta gccaacaata ttggaataca aattcgagat atgacagaaa gtgaagtcta
     2161 tcaaagagaa ttaggacaag cacggggggc gtatgttcgt aacgaattta ctggcgaaga
     2221 agaaatcgtc atgaatactc gaaatactcc tacgcagcat ttagctacaa gtattcatga
     2281 attagcccat gcaagaatgc acaaattttc agaattagat accgcaacta aagaattgca
     2341 ggcagaaatg acttcttata ttgtgtgcaa acattttgga atggacactt cggagaaagc
     2401 tattccttat attgcagcat ggacgaagaa tggtcagacg ttggatgata aggaagcggc
     2461 ggaacgtggg aaaatcatga atgatgtcag ccgtgtcgca aatgaattca ttcaaaccat
     2521 ttctaatgaa atcaatcaat acagagaaag agaaccagag atccaaccag cagaagtgaa
     2581 gcaggagcat aaaaatactg tagaaacggc agagaataag aatattgtgc ccgttggttc
     2641 actttggatc gacaagaaag acgggaaagt aatggaagaa gatacgggtc gaacgttaca
     2701 tttgaaggat gcggcgttta ttgaagatcc gcagcataat caagtgattt taaaaggaaa
     2761 aagttatgga aaagagattc aaacggcgtt accttcagaa gaatttgcgc taggaaatcc
     2821 attccgattt tcacttgaaa cagtgtcaga agtggccaaa cgaaaaaatg aaatctatta
     2881 tgaacaacaa ttgtcacaaa aagaacgcgt ataaaacaag taaaaaagac tatctgaaaa
     2941 acggatagtc ttttttcttt tgggaggttt gaaaatgacc gaaaaaaaaa tgagtcttat
     3001 tgatcgatgt aaacagatcg atatagtgga ttttgctcga aacaatggaa tggctgtagt
     3061 caataaagga cgagactatc gcttagaaga ccatgattca tttgtttttg atcgaagaaa
     3121 acaacgtttt tattggaaca gtcaaaatat tagtggcgac attattgaat tagctaaact
     3181 ttttttcata gacaaagaga tccaagactc taagcaacaa tttaaagcag ccacagattt
     3241 tattctaaag aatgaagaca aaacagaacg agtagaaaat cttcattttg agacagaaaa
     3301 gtacaaagat caccccgttg attatcaacc actcacagaa aaaggaagga attatctgaa
     3361 ggaagaacga aaactaccag agtggctgat tgattatgca gaaaaagaag ggttgattgc
     3421 cgaactaaag ccaaaacatg aacggcaaaa tttcttagtt cgagacgatc gtttagatca
     3481 tgcggtcgcc tttttatgga aagatccaca gacaaatgag acagtagggg cgagttatca
     3541 aggaacgttc attgattatg aacgttttgg tgagagaggc acatataagc atatagataa
     3601 aaattcgaca gcgaatcatg gctttaacct aaagatcggt gaccctaagc agttaaaatt
     3661 ttttgagagt agtattgatt tacttagcta tgctgcactg aatcgtgatc agttaaacga
     3721 tacgtggtta gtttcgatgg aagggttaaa acaccatgtg atcagtcatt attttggtga
     3781 ggcagtatct gaattgagaa agaaacaagc ctttcctcaa tccatcgaga tttgtgtgga
     3841 taatgaccgt gcaggacata ttttctacga aaaagaacaa ttaatgggcg cagttgatcc
     3901 atttacgaat cagaaagtac gttgtgaacg aggaatcgct aatgattggc aagtgccaaa
     3961 agaatacaaa gtcatttatg aagaagtcgc aaaagaagaa aagatcacac cagaagctat
     4021 tatggccata cacaagacag aaaacaatct gcaattaacg aaccaattgg tttccgctca
     4081 caaagtaaat gcgtcttttg gtcagcagct ttctgttaac gattcaatag aagctattaa
     4141 tttgaaagat atttgtagag aagtcgcaaa agaattaaaa gcttgtgaac gctcgaatgg
     4201 tacgtacgat tttgatcgtt tttatcaagg aaaaggggat attaatgctc agattctttt
     4261 ctcttataaa gcagaacaat actacaaagg ctataaaaag catgaacatg aatttgtacc
     4321 tgaagtgaaa aaggattgga acgatcaatt gaagcatgag atccagcaac aagaaattag
     4381 aaagcaaaaa cgtgcgatgt tgtttcaaca aggcagacaa caagaaaggg agtgagatcg
     4441 tcacgatgaa aactgtcatt ttagcagaaa aaccgtcaca agcgaaggct tatgccgata
     4501 gcttttctaa agccacccga aaagacgggt attttgagat ccaagatcga ttgtttcctg
     4561 gtgaaactgt gattacgtat ggttttggtc atttagtcga gttagattcg cccgatatgt
     4621 acgatgaaaa ttggaaacaa tggtcattag aacatttacc tattttccct acacattatc
     4681 actatcatgt tcctaaagat aagaaaaagc aattcaacgt ggtgaaacag caacttcaat
     4741 cggccgacac cattattatt gcgacagata gcgatcgcga aggggaactg atcgcttgga
     4801 cgatcattca acaagcaggc gccgatcatg gaaagatatt taaaagactt tggatcaact
     4861 ccttagaaaa agaagccatt tatcagggat tccagcagtt acgagatgca gaagagacct
     4921 acccaaaatt tgaagaagca caagcacgcc agatcgccga ttggttgatc ggcatgaacg
     4981 gtagcctact gtatagttta ctcttgcagc aaaaaggcat accaggaagt ttttccttag
     5041 gcagagtcca aaccccaacg ctttacatga tttatcaatt acaggaaaag attagaaact
     5101 tccaaaaaga accatatttt gaaggaaaat cccaagtaat cgctcaaaat ggcgcatttg
     5161 atgcgaagct tgatccgaat gaaacccaag cgacgcaaga agcatttgaa gattatttaa
     5221 aagaaaaggg ggttcaattg ggaaaacagc caggtacgat ccaccaagtt gaaaccgaga
     5281 aaaaaagtgc ggcaagtcca cgtctttttt ctctatccag ccttcaatct aaaatgaatc
     5341 a
//
LOCUS       DAMYXM010000116         5215 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.120, whole genome shotgun sequence.
ACCESSION   DAMYXM010000116 DAMYXM010000000
VERSION     DAMYXM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.120"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            464..1375
                     /locus_tag="PNS21_002678"
     CDS             464..1375
                     /locus_tag="PNS21_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HDI5160312.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1862..2128
                     /locus_tag="PNS21_002679"
     CDS             1862..2128
                     /locus_tag="PNS21_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160313.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2200..2541
                     /locus_tag="PNS21_002680"
     CDS             2200..2541
                     /locus_tag="PNS21_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160314.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2596..3015
                     /gene="ssb"
                     /locus_tag="PNS21_002681"
     CDS             2596..3015
                     /gene="ssb"
                     /locus_tag="PNS21_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI5160315.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3092..3388
                     /locus_tag="PNS21_002682"
     CDS             3092..3388
                     /locus_tag="PNS21_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160316.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3403..3834
                     /locus_tag="PNS21_002683"
     CDS             3403..3834
                     /locus_tag="PNS21_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDI5160317.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4016..4321
                     /locus_tag="PNS21_002684"
     CDS             4016..4321
                     /locus_tag="PNS21_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160318.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
     gene            4725..5213
                     /locus_tag="PNS21_002685"
     CDS             4725..5213
                     /locus_tag="PNS21_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDI5160319.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
BASE COUNT         1953 a          752 c         1080 g         1430 t
ORIGIN      
        1 cactactaaa aatagtagta gcataaaatt taaaagggaa aaaatcaacc cctaccccag
       61 tcttggggtc tcaaaactaa ctccccccta accccctctt tcagcctctt ttgaaagagg
      121 gggagaatca accccacggc caacgcccca gtgttcttgg ggctgtccag aacagaaact
      181 tgcgagagag tgggggtaaa acaagtgtag aggaaacttg aagacttgtt cgaacagaaa
      241 cttgcgagag agccggagta aaacaagtgt agaggaaact tgaagacttg ttcgaacaga
      301 aacttgcgag agagttggag taaaacaagt gtagaggaaa cttgaagact tgttcgaaca
      361 caaacttaca agagagggga gtaaagcagt tgcagaggaa tcaggaagat ttgttcgaac
      421 agaaacttgc aagaaagcat tattaaattg aaggtgatga aatatgaagt atttattgca
      481 tggctatttt attgattatt ctactcgcaa tagtagtact ttagcgattc tatgtttgct
      541 tcatgaattg agagtggtta ctagaaaaca gctatacaaa ctattgaatt ttgacagatc
      601 aattagtaaa caaggagtaa cgaatgcttt agtgagatta tatgaaaaac agttaatcga
      661 tcgaaaaaaa tatggaacag aaatgtatta ttatttgacg aaagagggac atcaaagcat
      721 aggtgggtat tatacgttgc cgaaagttcc tgaatataat ttgaatcacc atttacaaat
      781 taacaattat ttgattaaag gaatggcatt ggcaaaagac cacccacatt taaaacggat
      841 tatttcagaa agacgtaccg tctatgaaac gaaggattta aatcctaaga caaaaggaag
      901 aaaatttttt gtgccagatt ttatttttcg ttttcgagac aaagaaaatc atgagatcaa
      961 ttggtccttt gaaattgagc taacgttgaa aacacgtcgt cgctaccgtg aagcgatttt
     1021 tccgaaatat attcgtgagt tgcgaacacg tcctgaatca catttaattt atgtgacacc
     1081 tagtggcgca attcaagaag aacttcatgc gtttaagcgt atttttttca agcgcgaaac
     1141 tgaaaaagat gcgagtcaac aagaattgtt tgagcggttg catatttttt cagctttaga
     1201 gtttgaagag ggattaaaag cattattaga gaatgatcca gcagtcaatt ggcggacagt
     1261 agaacgagaa aaaatttatc cgaatggcgc tgttcaaagt cgtttgaatt tatttgaaga
     1321 atcaactgaa aatgaggaaa aaccaaaagt gaagtttacg cctgaagtat tgtaaaagag
     1381 ggtgttcgct tcgctctttt tttgtgccag tttgctggca ccgttcagac gcaagcgtct
     1441 gaaccggctc gtttttagga aggactaact tgtttgattt atccgttgtc aaagaagtgg
     1501 acgttcgaga gtccctaatc gtatagcagt cattcggcga gtataaccta aaacataacc
     1561 tgccaattgc caagggtaaa atgcacgctg cgcgcccttg gcaatcgtca gaacatgttt
     1621 tcgagccggt tatgccgaac gaccgctata cgaaaagggc ctctaaacag aagtccactt
     1681 tgatccttga caactgaaac ccaaaatcaa acaagcaggc cttcgtctaa aaacgagccg
     1741 aatgaagacc ttgggggttg cacccccaaa cccccgtgcc cccgacacac taccgaaaag
     1801 gtgggggtgg gtggcgaaaa aatcaaataa aacattctat tcaagaaagg aagaaacaac
     1861 catgacaact aaaaacgagc aactgaaaga acaggcaaga aaagagttgc aagaaagagg
     1921 tttgattatt gaggggagct ttgaaggaga ttttgaaacc tatattggtt gttatgcaag
     1981 accgataaac aaacctacag ccttagaccc aatgaacgaa caagaagtaa aagaacaaga
     2041 aaaacatgca gtgaatggtt ttccgcaaga tttcactgaa tggtatgagt gggaaattaa
     2101 caacgggaag cttgaaaact tcttataaaa gtcagatgac gtgcaaagag aatgattctc
     2161 tttgtacggt aaaaatataa agaaagaagg aaaaagaaaa tggcaaaaaa cacaccttat
     2221 caagtaggag acatttttta tagtagttgg ggctacgaac aaacgaacgt gactttttgg
     2281 caaattgtga agttaacaga aaaaacagca tggtttcagc ctattcaaaa gaaaacggtc
     2341 gagcattatt cgtccatgag tggaaaagtt gttccaatga aagacaagtt tatagagtat
     2401 cctttagcaa aaactaaaga cagtattgta aaaaaacgaa ttgatccaga aacctatcct
     2461 gatatgttat gtggttatcg tagttgggac acgtttcata gatataaaaa tcaagctgtt
     2521 tatgaaagta gttattatta aggggatagt ttcccctttc aaaaataata aatagaagaa
     2581 tggagagaaa aaatcatgat gaattcagtg aatttagtag gacggttaac aaaagaagtg
     2641 gatttaaaat acacaccaaa aggcaatgcg acaggcacat ttattttagc tgttaatcga
     2701 aattatacga atgcaaacgg cgagcgtgaa gcagattata tccgctgtgt catttggcgg
     2761 aaagctgcgg aaactttagc aaaatatact ggaaaaggaa gtttgattgg cattaatgga
     2821 cgtcttcaaa cgaggagcta tcagaatcag caaggacaga ccatgtatgt tacggaagtt
     2881 ctagtgacag atttctattt gttagagagt aaagaaatca atgagcaacg gtcaaaatca
     2941 atggcgaata atcaaaataa ttcttcaatt ccaggaaatg agattcaaat ttcagatagt
     3001 tatttgccct tttaagtgaa aaataatttt gttctatacc tgatttcttc aggtatagaa
     3061 caaaaaataa atagagatga ggcgattttt tatgaaaatc attgacagtt acgatcaacg
     3121 aaccatgcaa tatttgggct cgtttgatta tacagatgaa aatattatta attatgttgc
     3181 aagtttacct ccttatcaaa gcgttcgctt ggtagatttt gcaagtgatg aattggtatt
     3241 aacaacgatg ggcaactttt tagattatgt cggagacaaa aaatggttgg aaaaaatttt
     3301 gtctaaattg attgcgaaac aaacaggaga agaacccata aatgcgattc aattgttcaa
     3361 tcgatatgaa caagaaaaca atgattaaag aggagagaaa aaatgaaaag attaaaacga
     3421 agtattttta ttgtgctagc attgattgat tttgttggga caggttgtga agcaattgac
     3481 gttcaaaaag ctcccttatc ggaaataaaa caagcaataa caaaacaaga tgaagaacaa
     3541 ccgctaaata cacaagtaga aatagtgaat caaaataaac cgagtttttc tgaagaagat
     3601 ttttcgttag aaaatggaac caggcaattt ttttcagatt tggatcgttt aaatcgggta
     3661 ggtgtagcaa gtgcactttt gcataaaagc aggatgccaa cagaagaacg agaccgatta
     3721 tacattaagc caactggctg gaaacaaaag aaaatgaaga atggcgatta tctttataat
     3781 cgttgtcatt tgattggcta tcaacgtgcg acaagtttcc ccataagtat gtaatacgaa
     3841 acgggaggat taatcttaag attaatcaca actaacctat ttgacagttg gaatgaaagc
     3901 tatcatgtct gctgaatcaa ctggtctgat actcctacat gcaataaagt ctactaggaa
     3961 gttattgtat gaagctaggt aaaggcggca gaacaatttc taagtgtgga atcatatgaa
     4021 gttggatatg ccgggacgct ataaattact cagggtgaga ctgtttttaa ttaaactgac
     4081 gaacttgcga atgtacgggt ctataaccga cttatattcg aaagatataa gattgcgaat
     4141 gcaaagaaca ttatcgaaaa gtaagaatgt tggtcatgaa attcgagatg gaagactagc
     4201 ggtgtatctg tcaagagttg gtaaaacatg tttgattgat tcagatgaga gtgggaatgc
     4261 gacaaaacgt tttacagaag aaatagagca actagcagaa ctgaatatac gatattttta
     4321 aatactttag cggagaataa aaaaatcctc tacagaaaac tacggagggc aacatgtgct
     4381 attgtttcaa caatacattt ttaattttta ccataacaaa aaagtacttg aaaaaaaaca
     4441 gaaatatgtt aaattaaaaa tgaaaaaatg gaagcggtta cttattcttt tgaatctaag
     4501 ataaaaattc aaaagaataa cataacccaa atatgattat acttgagatt ctaaatattt
     4561 atcgaaaagg aatttgtttt aaggaaaaat gattctatgg acggttattg atttatattt
     4621 accaaatggc taataaaata gtatttctta tattgtatag tttttgtttt tatgtgaaaa
     4681 catgagcaat agaattgtac tagtaggata aggatggatg gaaaatgaaa aaaactattt
     4741 ttgttttagc gatttttata tgttttgggc tacttttggt aggctacttg ttgtttaatt
     4801 ctaatgaaat gagaactatt tctacgaatg acttgttaaa aaaaagtgaa ttaaaggata
     4861 aaaaaaaaga atctcttgcc tttataatgt ttactcaaga agagtgtgag tattgtatag
     4921 caataaatcc aatcatagat agtgtaaata aggaaacttt gaataagata aagaaatatg
     4981 atatatctgt agattttcaa tctaaacaaa agtgtaaaat taagtttgtt ccgactattg
     5041 ttgcatatag taagaataaa gaagtgggga gaattgacaa tgaattagat aatttgtatc
     5101 gaaaaaagca agatggttca attgtaatag acaagaaaaa aacaaagaca ttgcttatag
     5161 attggatgaa aaaaatggat aaacaaggag aatatttgta tggagaagat taaac
//
LOCUS       DAMYXM010000117         5154 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.121, whole genome shotgun sequence.
ACCESSION   DAMYXM010000117 DAMYXM010000000
VERSION     DAMYXM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.121"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="PNS21_002686"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="PNS21_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HDI5160320.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3250..3768)
                     /locus_tag="PNS21_002687"
     CDS             complement(3250..3768)
                     /locus_tag="PNS21_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HDI5160321.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            4886..>5154
                     /locus_tag="PNS21_002688"
     CDS             4886..>5154
                     /locus_tag="PNS21_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI5160322.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAAL"
BASE COUNT         1724 a          839 c          796 g         1795 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcgtta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     3781 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     3841 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     3901 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
     3961 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
     4021 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
     4081 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
     4141 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
     4201 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
     4261 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
     4321 tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt
     4381 taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca
     4441 ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac
     4501 cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt
     4561 aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa
     4621 acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact
     4681 ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta
     4741 cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc
     4801 ccacttagct tggataggct acaattaact agacagaaaa attaaggtgt gtagactaga
     4861 agaaaacata ccaggaggaa tttttatgtc taagagaaca cgaagaactt tttcacaaga
     4921 attcaagcaa caaatcgtca atctttactt agctggaaag ccacgtgtag aaatcattcg
     4981 agaatatgaa ctaacggctt cagcatttga caaatgggta aagcaatcta aaacgagtgg
     5041 ttcattcaaa gaaaaagata atcttacgcc tgaacaaaaa gaattgttag aactacgtaa
     5101 aagaaaccag caattagaaa tggaaaatga tattttaaag caagcagcgc tgat
//
LOCUS       DAMYXM010000118         5112 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.122, whole genome shotgun sequence.
ACCESSION   DAMYXM010000118 DAMYXM010000000
VERSION     DAMYXM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.122"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(464..1408)
                     /locus_tag="PNS21_002689"
     CDS             complement(464..1408)
                     /locus_tag="PNS21_002689"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDI5160323.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1704..2506)
                     /locus_tag="PNS21_002690"
                     /pseudo
     CDS             complement(1704..2506)
                     /locus_tag="PNS21_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2727..3941
                     /locus_tag="PNS21_002691"
     CDS             2727..3941
                     /locus_tag="PNS21_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5160324.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3963..>5112
                     /locus_tag="PNS21_002692"
     CDS             3963..>5112
                     /locus_tag="PNS21_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-rhamnosidase"
                     /protein_id="HDI5160325.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIY"
BASE COUNT         1533 a          921 c          906 g         1752 t
ORIGIN      
        1 attttagttt acataatata tattatacaa agtagattta aaaaatgata aacctctctc
       61 gaacggtttt ttatcaaaaa tcgtcatttt tccatcaaaa tctgcttatg caaaattata
      121 aaaaaatttt gcagtttttc ttactttttt tgtcgtttaa ttgaatgata aaaaccagtt
      181 tctttattca gttaatcaaa tatgaacgaa ttagatatga atcattgtat ttgccgttgc
      241 ggaaggattt tttaattaac cacagataaa cattgataac gattcgctta ttgtaatcaa
      301 cagattaaat ttcatatgtt catatgtacg gatacatttt cggaccgatt aattttaacc
      361 attctgttta tttgcttacc tattagatat caataaaaaa ctgcttaaca gctatttcag
      421 ctgttaagca gttttaggat ttttagttca tattcattgt ttcttataat acagtgttca
      481 atacttctct taaagcactt gttgatttgc tgaacattgc tttttcttct tcgttgattg
      541 ataattcaac aacttcacga acaccatttt cgtcaacgat tgatggcaca cctgtaaaga
      601 tatctttttc gtcgtattca ccagttaaat aagcagatac aggcaataca gcttgttcgt
      661 tattcaagat agcttttacg atacgtgcag tactcatacc aataccatag taagttgctt
      721 ttttacgatc aatgatctca taagcagcat tacggacttt atcagcaatc acatctaatt
      781 catcttgggc aatacgatgg tctttttcta caatttcaaa cactggttta ccaccgacag
      841 ttgtatgtga ccatgcagca acttctgaat caccatgttc acctaagata tagccgtgga
      901 cgctgcgtgg gtcaactttt aatttcaatg cgatttcttt acggaaacga gttgtatcca
      961 atgttgttcc agtaccgata acacgtgatg ttggcagacc agattcgttc caagcgatat
     1021 atgtcaaaat atctactggg tttgaagcaa caacgataat accgtcgaat cctgatccca
     1081 tgatttcttt tacgatttgg cgcatgattg acgcatttgt tttaactaaa tctaaacgag
     1141 attgaccagg tttttggttg ataccagctg tgataacaac gatatcagca tctttacatt
     1201 cttcatagcc acctgaccat acagccacat tttcatcgcc ccaagccata ccatctaaaa
     1261 ggtccaatgc ttccccttct gctttttctt gattcacatc gatcaatact aattcattag
     1321 agattccttg gttgatcatt gcataagcga tacttgttcc aacaaaacct gtaccgacga
     1381 ttactacttt acgacttgtt tttttcatga ataaacagct ccttttcata actatttgtt
     1441 actttcatga aaagtatagt cttgtttatt cattttatca acatgttaat tataatagtt
     1501 gttattataa tttttgatgg aaacgaattc ctgaatacgc ataacagata aatgacaaat
     1561 gtttacacct atatcccctg ataatacaac caaaagttta gaactgaaaa taaatccaaa
     1621 aaaattcgct gcaacgatat aataagacag caatcgcacc taaaacaatt ttccttcata
     1681 tgctttagtg aaagattttc ttcttaatgt ttatttctcc attcattggg agtacatgtt
     1741 gaatacttct tgaataattt gaataaagaa gtcgtttgtt tatatccgat acgattagcg
     1801 atttcttcca acgaaaaact cgtttcttga atcagttcca acgcaaagtc gaattttttc
     1861 tttagaacca gttcttgaaa ggaagcatca aaatacattt tcaatttatt actaagatag
     1921 accggattgt agccaaaatg agctgctgtt tcttttaacg tacaagaagt aaaatgctta
     1981 tcgatataat ctaatactct aagcatctca ttttctgcgc atccatctgc tgatgaaaag
     2041 ctttgttgtt ctaataaatg agtcaattcg atcagtaata gtgatgtata gagatttaac
     2101 gaacggatct tatgactgta tgtatcaaaa taaatcaaca gcatattttc aatcaaatgc
     2161 gtcaaacgac ttgtttttcc gccgcgaaaa tgaatgaatt gtgtatgttc ccctttttct
     2221 tttgatgcat ttatcaaaaa agaagaaagt agattatttg tgcttgccaa atgattcaat
     2281 atctctgtag ataaggtttc ttcttttaat aagatgttga ttaaaatatc ctcttgccct
     2341 gtgtattcga ttctttgagg tgtttactga tatgaatatt tccctgttga aatagatccc
     2401 ctcggtggat ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca
     2461 ttccttgttc acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat
     2521 tcagctagtt ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca
     2581 cgctgtactg ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg
     2641 gacatgaact tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga
     2701 taagcgctta caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc
     2761 tgctgtcagt ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta
     2821 tgtcagctac ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct
     2881 cttttatggt cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa
     2941 aacaaactgt gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct
     3001 cacaaagtta cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga
     3061 tcaattcttt ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta
     3121 tgattggttt tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag
     3181 gacagctagt acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta
     3241 ttcttgtctt gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg
     3301 gcacagcccc aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt
     3361 ttctcttgct ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga
     3421 cgacttattc tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg
     3481 caagtatggc tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc
     3541 ttacactttc tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc
     3601 tgtatgtgat tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt
     3661 acttattcaa ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa
     3721 tcttattggt tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct
     3781 cttttgcttc tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt
     3841 tgattagcgc gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa
     3901 gagtagatga agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta
     3961 aaatgacaaa tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc
     4021 gccatggact gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact
     4081 ggtacatgga tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac
     4141 cttccatgtt taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac
     4201 atccatgtgg aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag
     4261 gacatacatc agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg
     4321 gctggacagc aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg
     4381 atatcgcaaa agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg
     4441 aaacggagat tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag
     4501 aactaatatc tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg
     4561 tagaagctat ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga
     4621 aagctcgtaa acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc
     4681 aacttcaagt ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt
     4741 cttctttact gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt
     4801 tgttttttat cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat
     4861 cattcatgat caatcaatat cttttttttg acgaggaaat caataaacga acgatattag
     4921 cattaagagg acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct
     4981 tgtggttgat tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca
     5041 tcgtctaccc taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag
     5101 ggtttattta tg
//
LOCUS       DAMYXM010000119         5004 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.123, whole genome shotgun sequence.
ACCESSION   DAMYXM010000119 DAMYXM010000000
VERSION     DAMYXM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.123"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..182)
                     /locus_tag="PNS21_002693"
     CDS             complement(<1..182)
                     /locus_tag="PNS21_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDI5160326.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="PNS21_002694"
     CDS             517..951
                     /locus_tag="PNS21_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDI5160327.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="PNS21_002695"
     CDS             974..1294
                     /locus_tag="PNS21_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160328.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="PNS21_002696"
     CDS             1435..1626
                     /locus_tag="PNS21_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160329.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="PNS21_002697"
     CDS             complement(1697..1954)
                     /locus_tag="PNS21_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDI5160330.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="PNS21_002698"
     CDS             2112..2375
                     /locus_tag="PNS21_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160331.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="PNS21_002699"
     CDS             2399..2587
                     /locus_tag="PNS21_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160332.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="PNS21_002700"
     CDS             complement(2670..2855)
                     /locus_tag="PNS21_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160333.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="PNS21_002701"
     CDS             3070..3441
                     /locus_tag="PNS21_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDI5160334.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="PNS21_002702"
     tRNA            complement(3591..3663)
                     /locus_tag="PNS21_002702"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="PNS21_002703"
     CDS             complement(3709..4137)
                     /locus_tag="PNS21_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160335.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="PNS21_002704"
     CDS             complement(4197..4955)
                     /locus_tag="PNS21_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDI5160336.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1743 a          784 c          978 g         1499 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt attt
//
LOCUS       DAMYXM010000120         4805 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.124, whole genome shotgun sequence.
ACCESSION   DAMYXM010000120 DAMYXM010000000
VERSION     DAMYXM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.124"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(81..1889)
                     /gene="pepF"
                     /locus_tag="PNS21_002705"
     CDS             complement(81..1889)
                     /gene="pepF"
                     /locus_tag="PNS21_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDI5160337.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2065..3063)
                     /locus_tag="PNS21_002706"
     CDS             complement(2065..3063)
                     /locus_tag="PNS21_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDI5160338.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3289..3942)
                     /locus_tag="PNS21_002707"
     CDS             complement(3289..3942)
                     /locus_tag="PNS21_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDI5160339.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="PNS21_002708"
     CDS             complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="PNS21_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDI5160340.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1413 a         1007 c          745 g         1640 t
ORIGIN      
        1 aaaaaaatac gtcgactccc ttacaagaga gccgacgtat ttttttcgtt tctattcgag
       61 aaaatcaaaa atagaaagcc ttataaacga tcgatcaatt cttctaattc atttagacgt
      121 tgttcaaaca tcgacattgc atcttcaata tatgcagcct gtgtcatatc aactcctgct
      181 tttttcatta cttccacagg ataatcgctg ttacctgctt ttaggtaagc caagtagttc
      241 tctagtgcct ctggttcctg gtttagtatt ttcttcgcca gtgctgaagc agcagaaaag
      301 ccagtcgagt attggaaaac atagtaatta taataaaaat gcggtatccg tgaccattcg
      361 aatttaattt ctgggtcttc ttcgactgct ggaccataat attttgcatt caatttacca
      421 taactatcac ttaggtattc actagtcaat ggcacacctt tttcatcttc cgtatgcatg
      481 aaatgctcga attcagcgaa ttgtgtctgg cggaaaactg ttcctttaaa cccatccaag
      541 taatggttga gtacataagc ccgtacacga gggtcttttt ctgtttccaa taaatactcc
      601 gttaggatat tttcattcgt tgtcgaagca atttctgcta aaaagatgga gtagtcgccg
      661 tacacataag gctggttcga acgagtgaaa taactatgaa cactatgtcc catttcgtgg
      721 acaagcgtaa atagctgatc cagcgtatca tgccaattca ataaaatata tggatttgtg
      781 tcatagcttc ccgaagaata agcaccgctt cgtttccctt tattttcgac aacatcgatc
      841 caacgttcag agaatgcttt ttctacgatg gccatgtatt cttcacccat tggtttcagt
      901 gcttctaaag ctttttcttt tgcttcttcg tacgtaaagg taattggagc ttcccctaag
      961 accggtgtat aaagatcata catgtgcagt ttttccactt ctaataaacg tttccgtaat
     1021 tccatgtatc gatgtaacaa aggcaaatgc ttgtttacca cgtctaccaa agtatcgtat
     1081 acactttcag gaatatgatt attgctcaaa gacgcttctc tggcagagct gtaattacgg
     1141 actttcgctt taaaattatg tccttttata tgtgtgccta aagtagaagc aaatgtattt
     1201 ctaaattgtt cgtaaacact gtacaacccc ttaaatgctg cttcgcgcac ccttcgatcc
     1261 gtgctttcta gcaactgacc atacacgcca tgagataatt ggactatttc accattttct
     1321 ccttcaatcg ttggaaaaac tagatctgca ttattcaaaa cagcaaatgt atcacttgaa
     1381 gcatcaaaga tttcacctgc tccagcaaga agagattcct gctcagcaga taagacatgg
     1441 gctcgattat ctactatttg ctggatataa tggcgataga cttccaattt tggttcttct
     1501 ttgaaatatt gccaaatctg gtcatctgac aattgcaata tttctggttc aaaccaggaa
     1561 acggcttcac taactttcga aaacagactg cttgctcttg cataaagcgc ttggtaatct
     1621 gtatttccag tgtcttgatc gtttttaagg tgcgcatata cataaatgac ttcagtttgg
     1681 cgataaaccc tcaatacgaa ttcaatcgca tttaaaaatt gagaagcgcc ttgatcaagt
     1741 gttcctttgt gtttttcaga ttgttttaac tcttctgata attccaaata tttctcatca
     1801 aactcttggt cgcttgaaaa gatcttggtc aagtcccaag ttaaattttc aggcaattct
     1861 tctcgttttg gcaactgctt tacttccatg aaatccactc cttttcttcc cttagctcta
     1921 tctatcaaaa tagatcatag tctaacatcc acatgtcaca gcatctaact tttctactca
     1981 ttcatgtgtc tgcttccatc aattttagca taactaaatc ttgttttaca tagaaaaccg
     2041 attcctagcc tatttttata cttactattt tttcaaagaa gacaagtgta tgcattcgtt
     2101 aaaaaactgc tgatagacca tctcttcatc aatcatagga aaaagccatt cccttttatg
     2161 ttcttttatt tggcaaagcc atgagttgaa atcaggaact ttctggtttt ccaaggcttc
     2221 ttcgtacaac attcgataaa gaaaaacctg ttcttgtaaa taaaagaaaa accgactttt
     2281 ttgatagatc cacgatgata gatgcaaaag atgtttatga cgcaagtagc attgttcttg
     2341 tatggatact gtattctttt gcttgtgaat aagttggcgt gtcagccatt tcttcccatc
     2401 ttcactcact ttttctattt ttttcatttg gttcatttta atttcgttac tagtgaatat
     2461 ttctttaagg tccctactag aaaaagtcca gcagctactg caaaaggaaa tatgccctga
     2521 tacagtctct gtaatatgat gatacaaata aagcttttgt ttcttgagat cagctttcca
     2581 gcaatgcact cctctttttc tattatagta gcagcaactt ttttctatca atgagaattg
     2641 cgattgtcca agaaaagaat taccaagtat ccaccatact tggtaactgt tattcaaata
     2701 attttgggtt ctttcaataa atctctggtg agacagtcta gaacattgga tttctattgc
     2761 tagattcgcc aacaaaatat ccggcctttg ccgcaacctc ggcaaatagg cttccaaaaa
     2821 tactggttct actgactgtc ccagccaatc catgaagcat tcctttaact gtaagtgctc
     2881 ttttgattct ctttcactaa agaccgaaca gttatctgat ttttgatggg caaaatgagc
     2941 acattttttc tttccctttt tcaaaatcac tctttttcga cagccacgac aataatattt
     3001 ctttcctttt tctgcatgtt tagcaaaaca gcacttatta tattcattca ttgcaagcag
     3061 catttttctc cctctgaact tatttacgtc taagaattta tttacacgaa aaaagagggc
     3121 gtgaaaaagc tattctgcat caagtaataa gaacagctaa acaaaaatag ttcccaatat
     3181 ttttcgtttg gttgggcttg ttaccgcaga tgcaagcttt tgaacacatt tatacgaaaa
     3241 aaagactgcg acacaatttt tgtcacagtc tcttactata atgctgattt atacaaaata
     3301 aaatttcgtt cgctcgatag cattacgcgc catcagacat tgtccatatt cattcaatgt
     3361 ttctggtgta cgatcgctct tttcagcaaa ctccaaaatt cgagcaatct gattctctac
     3421 atccgcttgg ttgagatttt ccatccagaa gtgaacgact aggtaatagc gctcttcgaa
     3481 taaatacaaa tcagtccaag cagactctag ttcgacatct gttgcaagtt ggatcacttg
     3541 ttcaaaatct tcaaaagcaa aagttgcttg tctttcctgt tcttctaggg cgcttccaga
     3601 taattcttcc tccggatcgc tgccaataat ctgctgacga agaaagtcac tcacatcttc
     3661 tgaatcaaca gtagaatctt ctgattgaga aatttcttca tccggaagat ttttactgat
     3721 aaacagttct aatccgtctc cttttggcaa aacttggaaa gtgacagctt cgcttcctcg
     3781 gaactcctct tcgatatcaa cttcttccaa aatactataa aagaaatttt cgatttcttt
     3841 gtgattgcca agtaaatcca aaaatgaaac gccccgttcc tctaaatctt catgaccaat
     3901 tagtacacgg atcgtatttt cattaatatg ttccatttcc attttgctac acctcacttt
     3961 gccacaatgt ttaatataga ttcattgtag cgaaatcagc ttaaatgtaa agaaaaaaaa
     4021 tctcgctgtt aagcgaattt aataaaacca gcggattttt caacctttca agctatctta
     4081 tttaactata acatttttgg cagacgaata ctatatagcc gacttttcag ctaattttcc
     4141 ctctctattt ttcgtgcaat catcatcttc tactaccttt ttatacatct aatcaagaat
     4201 actttttgta aaaacaaaaa aacttggaaa aactatcttt gtaatagtct tttccaagtt
     4261 tcgtattatc cttctataat ccagccatca attgtgcttg acgtaattct aattgtcgta
     4321 catcgcgcgg taggaaccga cgaatttcat cttcattaaa accaacttgc agacgtttat
     4381 catcaatcat gattggacgg cgaagcaatc caggattgtt ttgcacaagt tccaataatt
     4441 cttgtaacgg cagatcatct aaatccatgt tcagtttttg gaatactttg gaacgtgtcg
     4501 agatgatttc ttctgttccg tcttctgtca tttgtaaaat cgcttttaat tcagaactgt
     4561 tcaatggttc tgaaaaaata ttacgttcaa caaatggaat ctgatgttct tgcaaccacg
     4621 cacgagcctt tctgcaggaa gtacaacttg gggaagtata taatgttaac aacgtgcacc
     4681 actccttttc gtgataagaa tcactttttt gtaacagaag atcataataa tctcccatct
     4741 gtttctatta tacataatca acttttaaat agctaccttt ttttcataat ttttttaaaa
     4801 aaatg
//
LOCUS       DAMYXM010000121         4773 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.125, whole genome shotgun sequence.
ACCESSION   DAMYXM010000121 DAMYXM010000000
VERSION     DAMYXM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.125"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            526..2337
                     /locus_tag="PNS21_002709"
     CDS             526..2337
                     /locus_tag="PNS21_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160341.1"
                     /translation="MNENGEFEESKKYQATSEEEKAAVRELLEEAVPTDFLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVREIEATPEDKKR
                     IESGQIEVIKKTYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQ
                     AVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIH
                     ELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTLEKAIPYIAAWTKNGQTLDDK
                     EAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAEQKNI
                     VPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSE
                     EFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2398..3867
                     /locus_tag="PNS21_002710"
     CDS             2398..3867
                     /locus_tag="PNS21_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDI5160342.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3878..>4773
                     /locus_tag="PNS21_002711"
     CDS             3878..>4773
                     /locus_tag="PNS21_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDI5160343.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMN"
BASE COUNT         1831 a          786 c         1029 g         1127 t
ORIGIN      
        1 ttgggaaaat ccaaaagaaa attagtacga ttgcaaaatg aattaaacga acaaattgaa
       61 aagatgtttg aacaccagag aaagaccaac ggtcaaccaa tgaacgataa gagaaatggg
      121 cattcttggt ttaggcaaca agagcgaatc gaaaataaag tgcatagtct gagagaagag
      181 atcaagcaac aagaaaaaca agtagaaaaa cttgaaagac aagaagagat aaaggaaatg
      241 ggctataaca agtacggcgg actggatatg acgatcgaga atattccaag aatcaaagaa
      301 gaaatcgaac gctttgaaaa aggtgaatcg actttttcag ccgctacgat ccgaaaatac
      361 caacggaaat tggaaacatt agaacagctg aaagaaagat cggaaaaagg aaaagaaaac
      421 cttcttcctg aagtacaagc aatcattgac agcggacgag tgacgcaatg gaaaaaaaat
      481 ccgaccattt attttctgaa aggctatcga aaagtcgctt tagaactgaa tgaaaacggg
      541 gaatttgagg aatctaaaaa ataccaagcc acgtcggaag aggaaaaagc agctgttcgt
      601 gaattacttg aagaggctgt accaactgat tttttgaagg agaaagaaaa cgatcatttt
      661 tatcgagtag aattcaacga aaatgatcca gaaaaaggat tgaaaagtta tggtggggag
      721 atcgtaacat tcgaattgat cgatcaactc aaaacgttgg ataaccaata cgccgcagaa
      781 gcaggttatt ataaattcta ttttgaagaa gtgaagaacg gagaagtagt cgatcagatc
      841 cgaatggatc taggggatgg cgtcaacagt aatcaaccca tctatttaga gttagcaaaa
      901 ttgtgcgaac aagtacctga gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg
      961 aaagaacaaa tgcagtcgaa agaaacaaaa acaaaacaaa agggctatca aaaacgaacg
     1021 gcagaagaga ttaaacaaga agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa
     1081 aagcatactc attctcctga agacgtcaaa gaacttttga actttatgtc tcgttttcca
     1141 gaacgatcct ttagaaatca attattgatt gaggaacaat ttccaggtgc aacggcttgt
     1201 ttaggtcgtg ccgcgttgaa aaaagaagga atctatatca aaaaagggga acaagggaac
     1261 aagatctttg tcagaaaaac ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga
     1321 gaaatagaag ctacaccaga agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc
     1381 aaaaaaactt actacacgat tgaaaaagtg tttgatatca cacaaacaca gttaaaacct
     1441 gaagactatc cgaaaatctt tccgaaccgt gtctttgatt ttcaattgga taaaaaagga
     1501 cagtcagaac ttcaagctgg gattcaagcg gtagccaaca atattggaat acaaattcga
     1561 gatatgacag aaagtgaagt ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt
     1621 cgtaacgaat ttactggcga agaagaaatc gtcatgaata ctcgaaatac tcctacgcag
     1681 catttagcta caagtattca tgaattagcc catgcaagaa tgcacaaatt ttcagaatta
     1741 gataccgcaa ctaaagaatt gcaggcagaa atgacttctt atattgtgtg caaacatttt
     1801 ggaatggaca cgttggagaa agctattcct tatattgcag catggacgaa gaatggtcag
     1861 acgttggatg ataaggaagc tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc
     1921 gcaaatgaat tcattcaaac catttctaat gaaatcaatc aatacagaga aagagaacca
     1981 gagatccaac cagcagaagt gaagcaggag cataaaaata ctgtagaaac ggcagaacaa
     2041 aagaatattg ttcctgttgg ttcactttgg atcgacaaga aagacgggaa agtaatggaa
     2101 gaagatacgg gtcgaacgtt acatttgaag gatgcggcgt ttattgaaga tccacagcat
     2161 aatcaagtaa ttttaaaagg aaaaagttat ggaaaagaga tccaaacggc gttaccttca
     2221 gaagaatttg cgctaggaaa ttcattccga ttttcacttg agacagtgtc agaagtggca
     2281 aaacgaaaaa atgaaatcta ttatgaacaa caattgtcac aaaaagaacg tgtataaaac
     2341 aagtaaaaaa gactatctga aaaacggata gtcttttttc ttttgggagg ttttaaaatg
     2401 accgagaaaa aaatgagtct tattgatcga tgtaaacaga tcgatatagt ggattttgct
     2461 cgaaacaatg gaatggctgt agtcaataaa ggacgagact atcgcttaga agaccatgat
     2521 tcatttgttt ttgatcgaag aaaacaacgt ttttattgga acagtcaaaa tattagtggc
     2581 gacattattg aattagctaa actttttttc atagacaaag agatccaaga ccctaagcaa
     2641 caatttaaag cagccactga ttttattcta aagaatgaag acaaaacaga acgagtggaa
     2701 aatcttcatt ttgagacaga aaagtacaaa gatcaccccg ttgattatca accactcaca
     2761 gaaaaaggaa ggaattatct gaaggaagaa cgaaaactac cagagtggct gattgattat
     2821 gcagaaaaag aagggttgat tgctgaacta aagccgaaac atgaacggca aaatttttta
     2881 gttggagacg accgtttaga tcatgcggtt gcttttttat ggaaagatcc acagacagga
     2941 gagacagtag gggcaagtta tcaaggaacg atcgtcgatt ttaatcgttt cggtaaacgg
     3001 ggaacgtata aacacattga caagaatcct acgccaaatc acggatttaa tttaaaaata
     3061 ggcgatccga agcatttaaa attctttgaa agtagtattg atctgttaag ttacgctgca
     3121 ttgaatcggg agaaactaca agatgcctgg ttagtttcga tggacgggtt aaaacatcat
     3181 gtaatcagtc attacgttga agagtcgatt tctgaattaa gacgaaaaca aacatttcct
     3241 caatcgattg aaatatgtgt ggacaatgat cgtgcaggac atatttttta tgaaaaagaa
     3301 cagatgaagg gaatcgttga tccttttacc aataagaaaa tccgttgtga gcgaggaatt
     3361 ccaaatgatt ggcaagtacc taaagaatac aaagctactt atgaagcagt cgcaaaagaa
     3421 atgagtgttg aacctgaagc aattatggcc atacacaaaa ctgaaactaa tttacagctg
     3481 acaaatcaat tggtgtctgc tcatgatgtt caatctacgt ttggaaagat gttggcgaag
     3541 ggcgaaccag tagaaacaat tgatttgaaa gaagcatgta ccacggtcgc gaaggaactg
     3601 aaagtatgtg aacgcgcaga tggaacatac aactttgatc gtttttatag tagaaaagcc
     3661 aacataaaag acgttaatgc aggaatcctt ctttcctata aagcagaaca atactacaaa
     3721 ggctataaaa agcatgaaca tgaatttgta cctgaagtga aaaaggattg gaatgatcaa
     3781 ttgaagcatg agatccagca acaagaaatc agaaagcaaa aacgtgcgat gttgtttcaa
     3841 caaggcagac aacaagaaag ggagtgaagt cgtcactatg aaaaccgtca ttttagcaga
     3901 aaaaccgtca caagcgaagg cttatgccga tagcttttct aaagctaccc gaaaagatgg
     3961 gtattttgag atccaagatc gattgtttac aggagaaact gtgatcacgt atggttttgg
     4021 tcatttagtc gagttagatt cgcccgatat gtacgatgaa aaatggaaac aatgggcatt
     4081 agaacatttg cctattttcc ccaatcaata ccactatcat gttcctaaag ataagaaaaa
     4141 gcagtttagt gtcgtcaaac gacagctgca gtcagctgat acgattgtga tcgcgactga
     4201 tagcgatcgt gaaggggagc tgattgcctg gtcaatcatt caacaagcag gcgccaatca
     4261 aggaaaaacc tttaaaagac tttggatcaa ctccttagaa aaagaagcca tttatcaggg
     4321 attccaacag ttacgagacg cagaagagac ctatccaaaa tttgaagaag tacaagcacg
     4381 tcagatcgcc gattggttaa ttgggatgaa cggtagccca ctgtatagtt tactcttgca
     4441 gcaaaaaggc ataccaggaa gtttttcttt aggtagagtc caaaccccaa cactctatat
     4501 gatttatcaa ttgcaggaaa agattagaaa cttccaaaaa gaaccatatt ttgaaggaaa
     4561 agcccaagta atcgctcaaa atggcgcatt tgatgcgaag cttgatccaa atgaaaccca
     4621 agcgacacaa gaagcatttg aagcctattt aaaagaaaaa ggtgttcaat taggaaaaca
     4681 gccaggtacg atccttcaag tcgaaacaga gaaaaaaagt gcggctagcc cacgtctttt
     4741 ttctttatcg agccttcagt ctaaaatgaa tca
//
LOCUS       DAMYXM010000122         4372 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.126, whole genome shotgun sequence.
ACCESSION   DAMYXM010000122 DAMYXM010000000
VERSION     DAMYXM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.126"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(83..1051)
                     /locus_tag="PNS21_002712"
     CDS             complement(83..1051)
                     /locus_tag="PNS21_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDI5160344.1"
                     /translation="MSKKRTIVNEWEKLTALSNRRLNVQTLVQTIKLNWSIDRITIVG
                     KLNQNIFYHTQHDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKW
                     QEGKGRIDFNPNKISRFLADSMKNFIHDLFLEPHFSRADVACDLIDVPDEFITQYRII
                     DPVSFKLIYGRSGKLETVYWGSRASERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQ
                     LRRGKATEWYAMVKESLDSFVSPHFLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYK
                     YRNLLKRESQNDELTNHLRETFEESADDLKKELDTWLQGLDVTEEE"
     gene            complement(1496..2902)
                     /locus_tag="PNS21_002713"
     CDS             complement(1496..2902)
                     /locus_tag="PNS21_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDI5160345.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSENEIEW"
     gene            complement(3124..3486)
                     /locus_tag="PNS21_002714"
     CDS             complement(3124..3486)
                     /locus_tag="PNS21_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160346.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3490..3828)
                     /locus_tag="PNS21_002715"
     CDS             complement(3490..3828)
                     /locus_tag="PNS21_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160347.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3807..4226)
                     /locus_tag="PNS21_002716"
     CDS             complement(3807..4226)
                     /locus_tag="PNS21_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160348.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4219..4359)
                     /locus_tag="PNS21_002717"
     CDS             complement(4219..4359)
                     /locus_tag="PNS21_002717"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160349.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1241 a          979 c          604 g         1548 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tactattcct cctctgtcac atcaagtccc tgtaaccaag
      121 tatccaattc tttttttaaa tcatctgctg attcttcaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcgcttta ataaatttcg atatttatat ttcgagtttc
      241 ttgacaaaat cgcccaatta gaatgattag ctaacaatcc atcaatcata actttatccg
      301 taggttttat atcagttggt aaaaaatgtg gactcacaaa gctatccaaa ctttctttca
      361 ccatagcata ccattccgta gctttaccac gtcttaactg catttctaac cgccaccaag
      421 tattaatttc tgaaggcacc acctttcttt tcatttcttg ctctattttt ttattgtaca
      481 aacgaatttg ccgctcgctt gcccgactac cccaatacac cgtttccagt tttcctgagc
      541 gtccataaat taatttaaat gaaactggat caataattcg atattgcgtg ataaactcat
      601 caggcacatc aataagatcg caggcaacat ccgctcgtga aaaatggggc tctaaaaata
      661 aatcatgaat aaaatttttc atggaatccg ctaaaaatcg actgatttta ttaggattaa
      721 aatcaatccg cccttttcct tcttgccatt tagaaatttc aatataggca atattttcgt
      781 catgttggtc taatagctgc caaccattat ttccaaccgc ttttaaatag ccattgcctt
      841 catttacccg cataagctgt tcaaaatcta atattaatac atcatgttga gtatgataaa
      901 aaatattttg attcaacttt ccaacaatgg taattcggtc aatcgaccaa tttaatttta
      961 tagtttgtac caaagtttga acatttaacc gcctgttaga cagggctgtt aatttttccc
     1021 actcattcac gattgttctt tttttactca tattcagttc ctcctcaaag aaaaaagaga
     1081 tttttggaaa gaatttccag agttcacgta actctaaaaa ttcataacca tcaatctcta
     1141 gtcttgaaga aacctaaaaa tattcgtata atgaatttga gttacggaac tagtgtggtt
     1201 agtttccgta aaggtcgaga acgttggtag cgttgctcga cttatttttt tgctcttttt
     1261 ccagttcatt tgtttgctaa ctcaataata gcacttctct ttagctatgc aaataccaaa
     1321 aaaataaaac cgcacccttt gaaaagggcg cccccaaact tcgctaacgc taagttggcc
     1381 aaaaaatgga cttgccattt tttagcaaat gtcagcttca ggggctagtg gtttagccgt
     1441 caactcctta tcctcggcac aaaccgcctc cggtaccgtt gactgcacat cgtggctacc
     1501 actcaatctc attctcagaa cgctctaatt tatgctgtga gacgttttga cgataaaata
     1561 aggttttctc ctgctctaat acttctaatt cctcagaaag ccccatatcc ttttctgcaa
     1621 gaccaaaacg gatagacaaa cgagtgatcg atttttgaaa tgcgtttttt aatcgctcta
     1681 aacgctggcg taaagagcga ttttcttctt tcagcttttt attttccact tgtaaatctg
     1741 ctaattgttt tctaccagac aaatgacgct cttcttccaa tgtagctaca tactcattta
     1801 attctgtatt ttgctcaagc atctgtaaat attgctcact catgttatca gcatttctct
     1861 tggcatttgt tgctctcgta gctcgttgat acaaatcaac aaaatcattc ccagataaat
     1921 gatacgaacc atcaggaagc ctcttgaaac tttctaaagg aaccatatct ttttggaaaa
     1981 tgaccgccat ctcttttttt acattcagca tatttttctt ctcttcttca atcttcgctc
     2041 tctcagaagc catcagagcc ttatcacgct cgttttcttc tctttgccta taaatatacc
     2101 tcaactcatc agaaacgctc tcacgctctt ttacagccgt ttggcgcaat tcagaaattt
     2161 cactcatgat ttctttgtat tcgtgaatat ctttaatttt gttggtaccg acaatatccc
     2221 ttttccaacc gtaatcattc attaatcgtt ctatagagtc aacttcttga tttctaaaat
     2281 tattgaaaag cactcgacca ttatctttat cagcgtgtat cccttgttga accagtgctt
     2341 tattaaagct cggctgcttt tgaacacccc gtttataacc ttcagcaacc ggcacaatat
     2401 tcaagtgcag gtgcggactt atttcgtcat tatgaataac agcattataa acagccaaat
     2461 tagggtttct ctcttgaaac tccttgtaat actgaaccaa aattttattg gcaatcactc
     2521 gattttgttc agcttgttgt ttcgatatgc cagtagaaga accatctgct cgatttgtct
     2581 ggaaatcatc taaaatgcct atttgcacaa taaattctcg ttgaggttcc aactttttat
     2641 ccttgcgcac tttcgctaaa tagtcctcga tttttctatc ggctcttttt tgtttcgcat
     2701 tatatttttc taccacttcg ccaaacaatt cctcatattt atctgctaga ttcgtctgct
     2761 ctaaataaat attttcatcc gatttcgtga agtcaatgtg cttatgccaa tcattggttt
     2821 tctggcgatc agttaactca cgattattat gagaaatact tgtattcttc gttccttttt
     2881 taaatgaaat actaaacccc aagttaaaac cctccttcgc gctgtcgcta ataaaaagat
     2941 tgtaaacctt tctgttactc tttgtcaaag agtaacattt tcctaattgt gacatggatg
     3001 tcacaattcc aaacgctctc cgagggtttt cgctgtcaca agtgacaacg aaaaaaggag
     3061 ccgaaagcgt atcacgctat caactccttt tatgctttat tttgcttcac gcaaaataat
     3121 cgcttatcgt aaattcacgt cattaaatat tgacgtaaat ttactctcac tcatctgact
     3181 tttcttattc ggtatcactc cgaataagtc gtcagattca tcgggataat ctcgttcgct
     3241 tggcgctgat ttttcatcag caagccactc attatccact gttttttcgt gttgctgatc
     3301 gtcagaaatt gacgataacc accgctcaaa atcagcaaaa gtttcaaacc caaaacgctt
     3361 gcgaagagcc ttgcccattg cagcattttc tttctcaatc aagcgttttt tctgttggcg
     3421 aagtttcgct tgttcttttt ttaatttttc ttcttgcgct ttaatatctg ctaaagtcat
     3481 tcccataaac tacatctcct tttctttgga attaataacg gaaatttggt ttaactgcat
     3541 aacatgactc attaatttca accaaaattc tttttctgat tcatcagttg tttgcgctaa
     3601 atgcttatga acaaattcat tagtcaaaac tgaagatttt gctagatcag cattgataac
     3661 cgagttgaga acatccataa ttcgttcaag catttcacta taattatctt catccggcaa
     3721 attcaaataa tcagctaacc gttcatacat caattcaatt ctatcgactg tttcttctct
     3781 caaatcaatt ttatccatgt cacacctcac ttccagaatt gccaccattt tcgatttttt
     3841 aattcttgat tttctagtaa caactgatcg tttttttgtt gctgaagcaa agtcaatttt
     3901 tgttggtcac gaatcatttc cgttaattca gaaatatgcc tatctttttc cgctaattgt
     3961 tcacgcaaaa attcattttc tttttctaac gggaaatcat tgcctgcatc attgaccggt
     4021 aaatcaccag tcaattgacc accttttgac cgataaattg accgcacaac accaatcaat
     4081 tccttgtcta acatgataac cccattatct ttataccgag caaggtcata acctgcattg
     4141 accagttgat tgcgcactgt atttttagat atacccaact catcagcaag gttttttatg
     4201 gtcatttttt ctggcaaatc agtcattatc ttctccgtta ctttcagcta ccccataaac
     4261 atatttaaca gcatgcttag ggataatgat tggggaatca attttattat ttttcaaaat
     4321 caactcatac tgcgttacat caagtaactt accggacatt tttttgctat cc
//
LOCUS       DAMYXM010000123         4262 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.127, whole genome shotgun sequence.
ACCESSION   DAMYXM010000123 DAMYXM010000000
VERSION     DAMYXM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.127"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="PNS21_002718"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="PNS21_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HDI5160350.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="PNS21_002719"
     CDS             complement(2020..2922)
                     /locus_tag="PNS21_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI5160351.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="PNS21_002720"
     CDS             complement(2919..3611)
                     /locus_tag="PNS21_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HDI5160352.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..>4262)
                     /locus_tag="PNS21_002721"
     CDS             complement(3654..>4262)
                     /locus_tag="PNS21_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDI5160353.1"
                     /translation="NLETITRAPGFFKYTEHTVAAHSYRVASIAQVLGDIEEANGQPI
                     DWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDEFINQEIPAEFQ
                     EVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKEMFIEAVTTIKS
                     FEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1305 a          911 c          751 g         1295 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 tt
//
LOCUS       DAMYXM010000124         4184 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.128, whole genome shotgun sequence.
ACCESSION   DAMYXM010000124 DAMYXM010000000
VERSION     DAMYXM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.128"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            77..1378
                     /locus_tag="PNS21_002722"
     CDS             77..1378
                     /locus_tag="PNS21_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDI5160354.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1405..1665
                     /locus_tag="PNS21_002723"
     CDS             1405..1665
                     /locus_tag="PNS21_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDI5160355.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2664..3899)
                     /locus_tag="PNS21_002724"
     CDS             complement(2664..3899)
                     /locus_tag="PNS21_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDI5160356.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1209 a          669 c          868 g         1438 t
ORIGIN      
        1 atataattag ttatgaacat tctttgcgtt gtcatgcaat gaatggttca tattttattg
       61 aaaaggagtg gttttagtgg aaaagctgat gaaatgggtc gaagagaaac ttgtcccacc
      121 aatggcaaaa attggtacac agcgtcattt attagcaatt cgtaatgggg ttgtttcaac
      181 attgtcattg attttgattg ggacattttt tatggtcttt atcaatttac cgtttcctgg
      241 atggaatgaa tttattgccc cttattcggc aacgattgtt ttacccttta ggatgacgat
      301 gggattgatg gctatttatg caacttttgt tatgggttcg gatctagcta aatcatatgg
      361 attagatagt gtgacaggtg gtatattatc tttaggaacg ttctttatgt tacaagttcc
      421 tgtaaacgtt ttaactcctg aagaggcacc tcttggctgg gtcttaccta tgagttcttt
      481 gggagcatct gggatgtttg cggggatctt atctatgatt tttgcagtgg aagtatatcg
      541 tttctttaaa aatcgaaata ttactatcaa aatgccagaa caagtgccac ctgctgttgc
      601 ccgtagtttt gaggcattga ttccaggtgc agtcatttta acaattactt ggctgattcg
      661 aagcgtcgcg ggctttgatg taaacgctgc cttattatcg ttattcgagc ctttaaccaa
      721 tattttaggg aataatttgc ttggtgtttt actaccaatg tttttgatcc atttgttgtg
      781 gtcatttggt atccatggca tgagtattgt aggggctgtt gttcgtccaa tgtggctgat
      841 catgttggat gagaatgcaa aggcattggc agatggtaca gctgccaccc aattacccta
      901 tatcgctcct gaacagtttt atcaatggac ggtaacaatt ggtggtgcag gagcaacgat
      961 cgtggtatct gttttgttta tattcttctg taaatcaaaa ttcttgaaag aggttggtcg
     1021 tttttctatt attcctggta tcttcaatat caatgagccc ataatttttg gagctcctat
     1081 gatgttgaat ccttacatgt tcatcccgtt caacttagtt cctttagttt tgactattat
     1141 cagttatttt gcagtcaaat tagaaatggt caatagattt actgttttgc caacatggac
     1201 attaccagct ccaatcggcg gttatctttc agcaggaaat gactggcgtg ttgttatctt
     1261 gatcatgatc aacacattga tagcatttat tatctattat ccatttgtaa aagcttatga
     1321 taaaaagatg ctgtcagatg aacaaagtcg tggagaaaac gaagtccaga ctgtctaagt
     1381 ttggttttta gaaggtggga aacaatgtat tggctgattt tattttttgt ttttatcttt
     1441 ttattaacgg ctagtcacct tatattgaat atgctagcta catatcatat tcaaatcaat
     1501 cgatggattt gggcactggc atcatttttg atcgtgattc ttccaaagat cattgtaccg
     1561 catatgaatg ttttgttttc atgggggaca tatgtacttt gtggaatttt tgctatcaat
     1621 tttatgatcg agcagcatag atggtttgtc acatcgaagc tttaaatgtt ctaaaagtgg
     1681 ttgtaacaga agtggttagc ttcaagaaat aagaagaaat ttacgaaaat tgcttcttaa
     1741 atttttgtga aatgttggct tatttttcgt ttggttgaac ttattaccgc agatgcaagc
     1801 ttttgaccac cgtttattcg gaatatggga ctgtgacaag gcttttgtca cagccctttt
     1861 ttacataaag attattattg ttttaactgt tttggcagaa agaaggagga aaaaatggaa
     1921 attttatcag caatggattt agaattagta gatctactac caaagtgatc aagtcaaata
     1981 tagatttggg agcttacaac catatagtag gggctgcagt atggtgtatg aatggtaata
     2041 tgtatgatgt gagtgtttat tcaaatcatg aaacgtgtat agaaataatt gtgctgggaa
     2101 tagcaatcag tcatggtgaa cgtgactttt agtgtatcgt tgctgacgac atagaacatt
     2161 ctgacatgat ctactcaata tgtggggatt gctggcagtt tatataggag tatgcatcgg
     2221 attgcgagat cattgtctca acagaagagg gaggttgatt atcaaagata aagtggtaaa
     2281 tcctacattt gatttagtaa ttgttgaggg gactagcggc acaaatctct acaaagcgat
     2341 gcttctagat ctatatgaca cattttaaga aaatgtattt gcctaattat tatggtcttt
     2401 cttttgaaga aacgttgaga tgatccaacc agagaaaggt gtcaaagaat ttgactaaga
     2461 ataaatggca gacgctacct atgctcattt ggatagcgtc agatctttta ttttaaggat
     2521 gattttgtgt tttttagaca ccgtaattta agtaactcta acttttcaat ccactattta
     2581 ctttttttgt attttcactg tctgggtctc gaatagcgaa attactgatg agtctctatt
     2641 aaaaaagctt tttctacttg aaactatatt gggtggttgc tcaggaaggt ttcgatttct
     2701 tgaattgctt gttgtggacc atttattgga aaaacggctt gcagcgtgcg tgcctgctga
     2761 cgaatcttgg gtgaagaaac ttcgcgcaga gcaatctgca gttttctttc agtcactggt
     2821 tttgtgaggg cgattgcgtt tccgtatttt acacaatagt caatattaca gctttgctca
     2881 aattgcatcc ccacaccgat aaacggtttg ccggattcac aggcagtttg gacggtccct
     2941 tccccgccgt ggataaaaga aaaatcaatg aattcatgca aaagatgact agacagatat
     3001 tctgttatta aaagatcttc tgagtcagat tgaatgtcat caagcagata gtcaataggt
     3061 gcaataaccg ctagatccgg ttgtttttgt aagaagaaca aaattttgcg taacatacgc
     3121 ggattgcctg aactacccat tgcgaaatag ataatacgtt ttctttcttg ttgctttcgc
     3181 tgtataaaag ttagcacttc ttcaggcaag gaagtactga gcttagcaaa aattggacca
     3241 acgattcgat aattttccgg cagctgttct tcttccaaaa atagtttagg cgttgtgatg
     3301 agatttagat cagcatccat catttgtaaa cttgtttttg gtaaaggaag catttctttt
     3361 tgtgcagcgt gtttgaaggc attggggatc catcgggtat ttactatacg cttttttgtc
     3421 cagtgccaaa ggggattgct taaagggccg aagaaacgta actcgtgggg catcagcgca
     3481 tgctctgatt gaaaaaaagg ttcactcaaa gcatagggtt taacatatac taaaggaatc
     3541 tttttattgc gggccgataa aaaaattgtc acattcgaac ctgtcacaat cagtcgaggc
     3601 tcaaacttag caatcagact ctgttcacta gtgatacgag aggcaaccat ttctttggta
     3661 aaagggtttt taagtgtacg caactggtca aattttatga tttgttcttc ttgttttttc
     3721 gtaaggatcg gcgctagtaa atgatactca aagccacttt ctgtgatcaa aggagcaaaa
     3781 gttttagaaa agccaaagaa tacacagtaa taacggtctt tcatagcttt ggcaacctca
     3841 atcattcgtg ttgtctcagc taaattgaat actgcaggag caaaaagaat tgtcttcatg
     3901 aaatcctcct tattggaatg agtctatccc ttcgtttctt actagtatac taaaaaacac
     3961 agcgttcgag tagcctatgt atattttgaa tattaaactg tctctcgctg ttttcacaac
     4021 ttctaaattt aaattttgta atcaagttga aactccaatt ttcaaaaaaa cacgcaacaa
     4081 ttttttgatc gatttatatg ttgtgccaga taaatacgag aagttgtgtc attaaaaata
     4141 aacatgagaa cataaaaaaa taaaataatt atattaattg atag
//
LOCUS       DAMYXM010000125         4039 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.129, whole genome shotgun sequence.
ACCESSION   DAMYXM010000125 DAMYXM010000000
VERSION     DAMYXM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.129"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            179..775
                     /locus_tag="PNS21_002725"
     CDS             179..775
                     /locus_tag="PNS21_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDI5160357.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="PNS21_002726"
     CDS             899..2698
                     /locus_tag="PNS21_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDI5160358.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="PNS21_002727"
     CDS             complement(2976..3188)
                     /locus_tag="PNS21_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160359.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3650..3799
                     /locus_tag="PNS21_002728"
     CDS             3650..3799
                     /locus_tag="PNS21_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160360.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         1187 a          688 c          712 g         1452 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctataatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaata
//
LOCUS       DAMYXM010000126         3916 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.130, whole genome shotgun sequence.
ACCESSION   DAMYXM010000126 DAMYXM010000000
VERSION     DAMYXM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.130"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(25..1530)
                     /locus_tag="PNS21_002729"
     CDS             complement(25..1530)
                     /locus_tag="PNS21_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDI5160361.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1527..2213)
                     /locus_tag="PNS21_002730"
     CDS             complement(1527..2213)
                     /locus_tag="PNS21_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI5160362.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2406..3827)
                     /gene="gndA"
                     /locus_tag="PNS21_002731"
     CDS             complement(2406..3827)
                     /gene="gndA"
                     /locus_tag="PNS21_002731"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDI5160363.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1082 a          837 c          676 g         1321 t
ORIGIN      
        1 aaaaaaacaa gtaaagcaca tgctttactt gtcttctgcc tttgcgatag gaataaagat
       61 tctaaaaatt gtcccttgcc ctactacact ttctgctaag atacgtcctt tatagttttc
      121 aaccagttgt ttagcgatag acaaccccag accatttcca cccttcgttc tagctcttgc
      181 tttatccact cgataaaaac gattgaagat cttattaagg tcttctttgg gtattccttc
      241 gccaaagtcc tgaatcgcga tctcaaattc attcagagtg gaagaaatag agatatggac
      301 ttcttttcgt gtagttgaat actttactgc gttatccaat atgataatga ttaattgttc
      361 aaagtgatta cgataaattt gcagggtcac ttcttttgtc aaatcatcat ctaaagtgat
      421 cacatagtct ggatataata tcttgaaatt attataaact tgataggtga cttcttttcc
      481 tttagttgtt tcattcgcat aatgaacatc tacttgttct gctcttgata agtctagcat
      541 ctcttgaact aagcttttca tacggctgat ttcttgcatg ctggcgctta atgattcttc
      601 taatatctca ggatcatctt tcccccaacg atttagcaga ctaagatgcc cttcaataat
      661 tgcaacaggt gtccttaact catgagatac gtcttccacg aattgctctt gttgttcaat
      721 atacaaacgc atacggtcaa gcatctcatt gaatatttca gctaaatctg ccaattcatc
      781 atttgtgtcg atttcaggca tgtggatatc tgattgcgga tctttacgta tagtatccat
      841 tgtatctctg agtaccttga gtggttttaa gaaataagaa gataaaaaga aacctaaaat
      901 actacttaag atcaaagaaa caacttctaa aacaataaga gtcaataata gcttatttcg
      961 aatatcatag aaagaagata attcatagaa gctttgcgca tagccgatct tttctcttgt
     1021 acctttagaa tatataggct ggatagataa aaagccagtt tttccttcta gtgtaacgat
     1081 cgttggaact ttacgtgtag tctgaatcag gtttcgactg ttttcatggg ttttaaaaat
     1141 caagttctcg tccaaatcat aaacataaag atccattgat ggctgcccta gctcagctat
     1201 aaaggaatcg atcttcatta agtttccttc gaccagtgaa gtatgatcta cgacctcatc
     1261 tccgtcattt gtcgctttcg tcagattacg atacgtattt actaatgtca gttcttcatc
     1321 cgagttagcc aaacgagaag ctacttctga aatcgtacgc tctacattgt ttctttcttt
     1381 tgccacaatc agattgatag atgatttata ggtaatgaca gcgaaaattg taaacacaac
     1441 gaatatgaag aaagaactcg caaaggccca cttgattgtc aaggagggcc ctttcagttc
     1501 tcttttttcc gattttttcg gctttatcac gagcgcatca cataacctgt tccgcgtaca
     1561 gtttggatat agctttcctc cccgggaacg tcaattttgt tgcgaagata acgaatatat
     1621 acgtccacta cgtttgtttc aacttctgtt tcgtatcccc acactttatt tagtaaaacg
     1681 tcacgtgcta ataccacgtt cacgttttcc attaaagtaa gcagcaactc gtattctctt
     1741 tttgttagtt caattacttc atttccgcga cgtacgacac ggttttcttt ttcgatagtc
     1801 aaatcgcgat aagtaatagt agtttgttta gccacatttt tatcgccttc aatatcgatt
     1861 cgacgcaaca atgcacgaag acgtgctaat aattcttcga tagcaaatgg tttgacgatg
     1921 tagtcatctg ctccgtggtc aagtccagat acacgatcga tcacagaatc tcttgcagtc
     1981 atcataatga ttggtgtatt tttgacttgg cgtacacgtc tgcagacttc taaaccgttc
     2041 aattctggga gcatcagatc caacaatatg gcatcccact cattatttaa tgccgcatct
     2101 aaaccagttc ggccgttgta gtggacttct gcgttatacc cttcatgctt caactctagc
     2161 tcgacaaatc gtgccaagtt cttttcatct tcaatgatta gaatattgct catttattat
     2221 tttatccttt ctattctact ggctttttaa aaactctatc attataccga taatatgctt
     2281 aaaaatctat agtcatttta tcatggatag caattttaaa aaagagctga agtctactca
     2341 taacaatcga cttcagctct ttcgtcctct ttttattttc tcatttccta acagccgaaa
     2401 gactattatt cttcatggta ccatgagtaa tggaagattc cttctttgtc gacacgctca
     2461 taggtatgtg cgccaaagta atcgcgttgg gcttggatta tatttgcagg cagacgttct
     2521 gctctgtatg aatcgaagta agcgatggct gatgagaaag tcggtacagg tacgccagct
     2581 tggacagcca aggcaacgac gtctctgact gattgctgat attttttcgt gatttcaacg
     2641 aagtaatcat ctagcaataa attttcgatc tcaggatttt tgtcataggc atccgtaatt
     2701 ttttgcaaga atcgagctcg gatgatacat ccagcgcgcc agatttttgc gatttcacca
     2761 aatggcaagt cccaatcaaa ttcttttgaa gcgacacgta attgagcaaa tccttgggca
     2821 taactcatga ttttactgaa atacaacgct tctcggattt tttcgaccaa ttcttttttg
     2881 tctcccgtga actcaaaatc gttcgtacga gacaaaatct tgcttgcttg tacgcgttct
     2941 tctttataag cagaaatata gcgggcaaat acagattcag tgatcaatgg aagtggcaca
     3001 cctaagtcaa gcgcgctttg gcttgtccat ttacctgttc ctttattccc agctgcatca
     3061 aggatcacat ctactactgg tttacctgtt ccttcatcgt ctttacgtgt cagaatgtca
     3121 gcagtgattt ctatcaaata gctgtctaat tcgccttgat tccattcttt aaagatatca
     3181 gccatttctt ctactgaaag gtttaaaata ttcttcatta agtcataaga ttcagcaatc
     3241 agttgcatat ctccatactc aataccgttg tgtaccattt tcacataatg tccggctcca
     3301 tttgggccaa tatatgtgac acatggtgct ccgtcttctg cttttgcaga aatttgctct
     3361 aagattggtg caactagcgc ataggcttct ttttgtccac caggcatgat tgaaggtcct
     3421 tttaaagctc cttcttcacc accggaaacg cctgtaccga taaagttgat gccagagttg
     3481 gctaactctt cattacgacg catcgtatct ttaaagaatg tatttcctcc gtcaatcaaa
     3541 atatcgcctt tatcaaggtg aggaagtaac tcttggattg ttgcatctgt tgccggtcct
     3601 gctttgacca ttagcaagat ccgacgtggt ttctcgatag attctacaaa ttcttcgatt
     3661 gtatatgttg ctttgaaatt tttatctggg tgttcttcta ctacagcggt tgttttagcc
     3721 cctgtacgat tgaataaagc cactgaatag ccgcggcttt ctatgttcaa ggctagattt
     3781 ttacccataa cggccatacc gacaacgccg atttgttgtt ttgacatatg aactcctcct
     3841 acatacggga tttctcccta atctcttaca ttatagctta acacggctac attgcaaact
     3901 aaagaacaac atattt
//
LOCUS       DAMYXM010000127         3879 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.131, whole genome shotgun sequence.
ACCESSION   DAMYXM010000127 DAMYXM010000000
VERSION     DAMYXM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.131"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(1..371)
                     /locus_tag="PNS21_002732"
                     /pseudo
     CDS             complement(1..371)
                     /locus_tag="PNS21_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(573..968)
                     /locus_tag="PNS21_002733"
     CDS             complement(573..968)
                     /locus_tag="PNS21_002733"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDI5160364.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            complement(978..1826)
                     /locus_tag="PNS21_002734"
     CDS             complement(978..1826)
                     /locus_tag="PNS21_002734"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDI5160365.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            complement(1841..2668)
                     /gene="panB"
                     /locus_tag="PNS21_002735"
     CDS             complement(1841..2668)
                     /gene="panB"
                     /locus_tag="PNS21_002735"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDI5160366.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            complement(2680..3540)
                     /locus_tag="PNS21_002736"
     CDS             complement(2680..3540)
                     /locus_tag="PNS21_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HDI5160367.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
BASE COUNT         1188 a          749 c          446 g         1496 t
ORIGIN      
        1 ttgataatag taggtctgac gagaaatatt tagaattttg cacatcgctg atatggaata
       61 tttatgcttg ttggcatcaa ttacttgtct tttcgtccga atatcagcgc cgcttgcttt
      121 aaaatatcat tctccatttc gagttgctta tttttctttc ttagtgcgat caattctgct
      181 tgttctggtg ttaagttgtc tctttttttg aatgagcccg ttgattgtgc ttgcttcatc
      241 catttatcga aagaagaagg cgtaagctca tactctcgaa taatttctgc gcgaggctta
      301 ccagcgagat agagattgac gatttgttgt ttgaattctt ttgaataggt tcttcgttga
      361 cgtcttgaca tgaaaaatcc tccagtgtgt tttttattat tctacacacc ttattttttc
      421 tgtctagtct agtgtagccg attcacataa aatggacttt gtctacagtc tgtatcttct
      481 ctttttgtgt ctactttatt gacatctatc caattagtaa tcaataaata tttcaataat
      541 agtctagcta tatataaata acattacaaa acctaaagaa ttatatctga taaccttcca
      601 tgtttttcat atgatgttac tctttcaact ttattattat catcgacaaa aacaacctta
      661 ggtctatgat ccttaacctc attttcattt aattcacaat atgccattaa aattatttta
      721 tctccagttg atacacatct agctgcagct ccattcaagc aaatcatccc tgttcctctc
      781 tctccagcta ttgtataagt ttcaaatcta tttccattat taacatcaac tatttgtact
      841 ttttcatatt catatattcc tgctgcttct aataaattca tatcaacagt aatacttcct
      901 acataatcta actcagcttg ttcaactgta gctctatgta tttttccttt taacattgta
      961 actttcatta tagcccccta ttgtaattca aatgtaaaat tatctattaa tcttgttttt
     1021 cctataaata ctgctattgc aactaaaact gatttttcaa tataatttac tctttgtaat
     1081 gataaagaat caacaacttc tacataatca atctttgcca aattataagt atttatattt
     1141 ttacttatta tctcaattat ttttaagctg tctctctctc cattatttaa agcttcctta
     1201 gctaaagtta aagatttatt taaaatagta gctgaagatc tttcctctaa acttaagtat
     1261 gtgtttctag aacttttagc tagtccatcc tcttccctta taattggaca ccctacaacg
     1321 tctatatcaa tattaaggtc cctaaccatt cttttaatta cagctaactg ttgtgcatcc
     1381 ttttctccaa aataagctct atctgctggt attatgttaa ataatttact tacaacagtg
     1441 caaacccctc taaaatgtat tggcctacta gctccacata aaccttctgt taatccagtt
     1501 atattaacaa aagtacttgc attgtcacaa tacatctctt caacttcagg attaaatatt
     1561 gctgttgctc tacctcttgt gcatacttct atatctcttt ctaagtctct tggataacta
     1621 cctaaatctt catttttccc aaactgagtt ggatttacaa agatacttac aacaacttta
     1681 tcattttctt ttgatgcttt tctaattaaa ctttcatgac cttcatgtaa atatcccata
     1741 gtaggaacta atcctacctt taagccttgt tttttccact cttttacttg acttctaaca
     1801 tcattcactg ttttaaatat aaccatattt cttgaccctt ttaatataat ttttctaaaa
     1861 catcatcact tattttaaac ccatgttctg gacctggaaa aactccatct tttacttcct
     1921 ctatatattt tgtaaatgct ttattcatct cttctgataa attagcatat ttcttcacaa
     1981 atttaggggt aaaatcacta tacatcccca acatatcttg atatacaaga atttgtccat
     2041 cacaccctgc acctgctcca ataccaattg taggtattga tatctcttta gaaattaact
     2101 ctgcaagctt agttggaaca cattctaaca ctacagcgaa tgctcctgct ttttctacag
     2161 ctttagcagc tcttattaat tctttagcag cttccttgtc ctttccttgt accttaaatc
     2221 ctccaaatgc attaacagat tgaggtgtta aacctatatg cgccataaca ggtatagaag
     2281 cctttactat agcctctatt ttatcgcata cttctatccc tccttctaat ttaacaacct
     2341 gagctcgtcc ttcttttatt aatcttcctg cattaactac tgaatcatat actgaagttt
     2401 gataagacat aaatggcata tctgcaacaa ctaaagtgtt cttagctcct cttgtaactg
     2461 ctctagtatg atgaatcatg tcttccattg ttactgaaag tgtatcttca tgtcctagac
     2521 ataccatacc aagagaatct cctactaata ttccatttat cccagcttca tcaattattt
     2581 ttgctgttga atagtcataa gctgtaagca ttgttaattt ttcatttctt aatttagatt
     2641 ctttaaatgt aactgctgta tttttcatct atttctctcc taactctctc ctaaatactt
     2701 ttctaattca ctataatctc tattaatatt ttttaacata gctatctcta atacattttt
     2761 tgataatagc atatataaag ctctgtcttc atctgataaa acttcacagt gacctttaat
     2821 tgttgaaata tcacaacgtt caactggccc tgtcaaactt tctataattc ctttttcttt
     2881 tatatttcta atattaaatt caattaatgg atataaagct tcaattgcca ttttttcagt
     2941 aaatccacat tgaattagat atgtaacgct tgtattaata agtcctagca caaggttact
     3001 agatactact gatgcggcat gataaagaga tttattctcc ttagatacaa ttacagtact
     3061 attacctaac tttttgaaga aatcacataa aaactttgca tatttttcat cgccttcaat
     3121 tgttataaaa gattttttta attttttgta tgaattatat ttatctgata ttgaaaacat
     3181 aggatggaca gaatagccat aagcaccata gtcctgtata tttgaaaaga tatctgaaga
     3241 tattgcacca ctgcaatggc agattaattt gtttttgatt tgataatttt taatttcttg
     3301 ccatacctcc tgaatttgac catcaggagt cgtaatgaaa atagtatcat tttcatctac
     3361 taattctctt aagtttaaat attgtttact agatgtaaaa ttcgaagctg ctaatgacga
     3421 gtcttcgctt ttactataaa atcctgttac ttgtatttta tgttcctgga aatacttccc
     3481 gaatgaacaa cctaccttgc cggctccaat aaaaccaacg ttcataatta tcacctccat
     3541 aaagaagtga gactacaagt cccattctta cgatatgagc ttaaaaataa aaattaaatc
     3601 aaatatacag tatgtttttt tcaaatatca tccatagtta atttatcata atatcccata
     3661 ttttaaaata aaagatagcg taaaatattt tgtgtaaata gaaatttagg gtagaaaaaa
     3721 aagtccattc tgtaaaatta aagttaccac acagaaattt tataggagga ctttctcatg
     3781 actaaattta ctatagaaat tatgcaaaca ctactcaata aaggggattt agatgaatta
     3841 tttcaggatc atttagagcg agcgattaat tcacttcta
//
LOCUS       DAMYXM010000128         3813 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.133, whole genome shotgun sequence.
ACCESSION   DAMYXM010000128 DAMYXM010000000
VERSION     DAMYXM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.133"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(208..756)
                     /gene="aac(6')"
                     /locus_tag="PNS21_002737"
     CDS             complement(208..756)
                     /gene="aac(6')"
                     /locus_tag="PNS21_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDI5160368.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1309..2412
                     /locus_tag="PNS21_002738"
     CDS             1309..2412
                     /locus_tag="PNS21_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDI5160369.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2605..3672)
                     /gene="rlmN"
                     /locus_tag="PNS21_002739"
     CDS             complement(2605..3672)
                     /gene="rlmN"
                     /locus_tag="PNS21_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDI5160370.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
BASE COUNT         1160 a          765 c          642 g         1246 t
ORIGIN      
        1 aatttatgca aaaatgtaga aagccatttt cacaaaattt ttcttactac tcgaaactaa
       61 gcgacttttt cacaacctct ggtacacggt gttccaaaag tagcttcttc ggaaataagt
      121 tggaatttca caaaaattca agaagtaatt ttcgtaaatt ccttcttatt tcttgaagct
      181 gattacttct gtcacaacct ctttttatta ttgagaattt ggtcgaggaa taatcgtttt
      241 tgccatccaa atatccggtt tgtcccagcc atttgcattt ggtaagacac ctacgatttt
      301 ataacctaat ttttcataga attcatacgg atgttcacga aggttctgga tggaagccac
      361 tttatcaaat gtatgctcat acaggtccgt ttgacttaac gttgttccat ggtctaaatc
      421 atccgtacct aaataaatcg tgattcctcc tctggaagct acttcttttt ctaagtaatt
      481 gactaatcga gtacctattt ggttctttcg tcgggagctt tctacaacta atggatgcaa
      541 ttcccaacct gtgataccgt attgagggat tgcaccaata aatcctacta actcatcttg
      601 gtctaccgct gctaccgcga ttcgttctgg attcatcatt tcttctactt cttctgccga
      661 gctgtctcca tattcttccg gccaagtcag tctcagtaaa tcagaaagct gatctttcaa
      721 tactggatta ttacggtcaa attcactgat tatcatacgt cttccccctt gttcttgctt
      781 attctaacag tccttttgaa aaaacgaaat gttctcgctc aaaaatcttt agctaccaat
      841 cagtatctct attctttcat tatttatctt acttataaaa taaaaaaatt atacagaaac
      901 actttattca aaataggaaa gatcaggaaa gccatcaatt ggaggaaaaa actctgtttg
      961 ttcatcatga tatagattat cttttgcttt aaaaaaaacc aattatgaat tgacaaataa
     1021 aacctgtttt catataataa tcttaatttt tagaattttt agataattac aaaaaagcaa
     1081 agggaatcgg tagaaatact aagaggaaca aaaattcatt attgtcgttt tcaatgtttt
     1141 tgtacttgtt cgtttttagg atacttattg atgatttcaa ggtgttcatg ccaccttatt
     1201 catcacttat cctgtcttag gttcacctct actccatcta cagccgcagt cagatcgatc
     1261 actctttaaa attttaaccc gtttcaacga agaaagaagg aaatgaatat gagtcaaagt
     1321 gttttcttac caaattatac cattggaaca gatgcgtatg aaaaagcaaa tggaatcgtc
     1381 aaagaatatg ggacaaaagt agtgctgatt ggagggaaga ctgcactttc aaaggcagaa
     1441 aacaaaataa aagtagcttt gacacaggaa atagaggttg tcgccagtct ctggtacggg
     1501 ggtaattcaa cttatggaaa tattgaacag ttgatccagc atgaaagtgt ccaaaaagca
     1561 gacgtcctgt ttgcagtcgg tggtggccgt gcttgcgata ctgtaaaagt cattggtgaa
     1621 aaactagcta aaccagtctt cacctttccc actattggtt cgaactgttc gcccgtcact
     1681 gcggtatgtg tcatctataa cgaagaaggc gtaatggacg gactcttctt tccttcaaaa
     1741 ccacctgtac acaccttcat tgatacagca attattgccg aagcacctga taaatactta
     1801 tgggcaggta ttggcgatgc tttatccaaa gaagtcgaat ctactttttc tgctcgtggc
     1861 gatgatctag gctacacgga tagattagga atccaaatcg gaaaaatgtg cattgatcgt
     1921 ctcttgactt atggtacgca agcattgaaa gatagtgccg acgatcgtcc ctcatatgca
     1981 attgaacaag tcgtttcgga tatcattgtt actaccggat tggtttctgt tttagttaac
     2041 aataactata attctgctat ggcacactca ttctattacg gtacgactgt tctagaaaaa
     2101 atggagggat accttcacgg agcatctgtc agttatggtg tactgacctt attgatgatg
     2161 gatcagcagt tcgatttgtt tgataaaatc tatgtgttta tgaaaaagaa ccaacttcca
     2221 gtatcgttaa ccgacatgaa tctcaatgtc acgaccgatt tagaagcaat cttagataaa
     2281 gccatggcaa caaatgatat taaacatgtg ccttatgaaa tcacccgaga aaaatttaaa
     2341 caggccattc ttgacgtaga aacttatcat gctacacaag aaagccagca aggatataag
     2401 aaaatcagtt aaataattgc taaaaccccc atcaagcagg ctgtcatcaa attgaggaca
     2461 tttctttgct tgatgagggt ttttcttgtc aaaaaaagct ttatagcttt cgctatagaa
     2521 tcttttctct tatttaagtt gaacagttcc ttaattaaga aaactgctcc ttttatggta
     2581 attgaaaaaa cgagtaggat acgattattt ggcaactgtt tttttcattt gcttgcttcg
     2641 caattgtccg caagctgcat caatatctgt accatgttct ttacgtatca cacagtttac
     2701 gccgtttttc ttcaatacat cataaaatct caatacatca gcttttggac tgcgagaata
     2761 ttgatcatgt tcactgactg gattatatgg aatcaagttg acatatgcta attttttctt
     2821 atcttttaat agatctgcta attgttgcgc atgctctggt ctatcattta cttgattcaa
     2881 catgatatat tcaaacgtga ttcgacggtt tgttttctct aaatactcat ccacagcttc
     2941 catcaatttc tcaattggga aacttcggtt gatccgcatg atcgatgttc gcacctcatt
     3001 attcggcgcg tgcaaggaaa tagccagatt gacttgcaag ccgttattag cgaattcctt
     3061 gattttgtgt gctaatccac tagtggaaac tgtaatgtgc cgagcaccaa ttgccaatcc
     3121 tttggcatca ttgatgatat gcaaaaagtc cattacattg tcgtaattat caaatggctc
     3181 accgatcccc attacaacga catgtgaaac gcgttcgcca agattgcgct catcaaaata
     3241 atgttggacc atcatgatct gtgcaacaat ttctccagca gtcagatcac ggttcttttt
     3301 gagcagcccg cttgcacaaa acgtacagcc gatattacat cctacttgtg tcgtcacaca
     3361 aactgacaag ccatattctt gtctcatcag tacagtctca atcatgtttt tatcagggag
     3421 ttcaaacaaa tattttactg taccatccga agcttcttgg acgaccatct gttttagagg
     3481 attgatgata aaatgctccg ataatttttc gatcaattgt ttcgatagat tcgtcatttc
     3541 tgtgaaatta cttacacgtt tttgatacaa ccattcccaa acttgggcag ctcggaattt
     3601 tttctcgccg ttttctataa accaattgat taattcttca ttcgtcaagc cataaatgga
     3661 ctgtttttcc aaagtatttt ccctcttttt ctcaaagttt cgctacatac tatacatgat
     3721 tcatttcctt aatgcaatac ttcacaaata aatcgaatag aactttaatc tcacaaaaac
     3781 taaaaagaca agacatcata ccgtattaga aaa
//
LOCUS       DAMYXM010000129         3801 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.134, whole genome shotgun sequence.
ACCESSION   DAMYXM010000129 DAMYXM010000000
VERSION     DAMYXM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.134"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            2..550
                     /gene="hslV"
                     /locus_tag="PNS21_002740"
     CDS             2..550
                     /gene="hslV"
                     /locus_tag="PNS21_002740"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HDI5160371.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            564..1964
                     /gene="hslU"
                     /locus_tag="PNS21_002741"
     CDS             564..1964
                     /gene="hslU"
                     /locus_tag="PNS21_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDI5160372.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1982..2773
                     /gene="codY"
                     /locus_tag="PNS21_002742"
     CDS             1982..2773
                     /gene="codY"
                     /locus_tag="PNS21_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDI5160373.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2855..3730
                     /locus_tag="PNS21_002743"
     CDS             2855..3730
                     /locus_tag="PNS21_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDI5160374.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         1301 a          661 c          853 g          986 t
ORIGIN      
        1 catggtcgaa tcaccatttc attcaaccac tatctgtgcc gtcgaaaaag acgggaaatt
       61 tgctatggct ggagatggcc aagtaacaat gggtgaatct gttgtcatga aaggcactgc
      121 aaagaaagta cgacgtatct acaatggaga agttgttgta ggtttcgccg gaagtgttgc
      181 agatgcgttt actttagaag aaaaatttga aggcaaacta aacgaatata acgggaatct
      241 acaacgcgca gctgtcgaat tagctcaaga atggcggaca cagcaatcta tgcaaaaact
      301 tgaagcgatg ctgatcgtaa tgaatgataa agaaatgctt cttgtttcag gtacaggaga
      361 agttatcaca ccagatgatg gtatcttagc tatcggatca ggagggaatt ttgcgttggc
      421 tgcagcccga gcaatgaaaa actatgctca aaacgacatg agtgcgaaag aaattgcaga
      481 aaatgcgtta aatattgccg cagatatctg tatctttact aatcataata ttatcgtaga
      541 agaactgtaa gaggtgagta gatatgtatg aattaaaaca aacaccaaga caaatcgtaa
      601 gtgaattaga tgaatatatc gtaggacagc acgcagcaaa aaaatctgtt gccgttgctt
      661 tgcgtaaccg ctatcgccgt ttgcagttag atgaaaaaat gcaacaagat attacaccaa
      721 aaaatatgct gatgatcgga cctacagggg tcggtaaaac agaaatcgca cgacgtttgg
      781 caaaaatcgt caatgcacca tttgtcaaag tcgaagcaac aaaatttact gaagtcggtt
      841 atgtcggaag agatgtagaa tcaatggttc gggatttagt agaaaatgcg atccaaatcg
      901 tagaaaaaga gcagtacagc cgtgtataca caagtgctaa aaaaaatgca gaaaaacgtt
      961 tggtaaaaat cttggtacct ggtatcaaaa aagaacagaa acaatccact aatcaatttg
     1021 aacaaatgat gaatcttttt aatgctagtc agcaaaatac cgctgaaccg caagaagaag
     1081 tgacagaaga tattcgaatc aatcgccgta cagtcttgga acaactacaa aagggtttac
     1141 tggatagtcg tgaagtaacc attgaagttg aagaccaaaa gaaacctgca ccaatgatga
     1201 acaatggatt ggagcaaatg gggatcgacc taaatgaaac tctaggtgct ttgacaccga
     1261 agaaaaaagt ggaacggacg gtaacagtca aagaagcttt agagttattg atcaaggaag
     1321 aatcagcaaa attagtcaag gatgctgata tccatagtga ggcaatcaaa ttagcggaag
     1381 aaagtggaat tatttttatc gatgaaatcg ataagatcac ttctaaatca cagcaatctg
     1441 gagaagtatc cagagaaggc gtccaaagag acatattgcc aattgtcgaa ggttcacaag
     1501 taaacacaaa atacgggatc atccaaacag atcacgtcct attcattgct tcaggagctt
     1561 tccatttgtc taaaccaagt gatctaattc cagaacttca aggacgcttc ccgatacggg
     1621 tagaattaga tgatttgaca gctgaagatt ttgtgaagat tctgaccgaa ccaaataatg
     1681 ctttagtaaa acaatacgtc gcgttgatcg gtacagaaaa tgtgtcagtt acgtttacaa
     1741 aagaagcaat tgaacggatc gctcaaatcg ctttcgatgt caaccgtgac acagataata
     1801 tcggcgcacg cagactgcat accattttgg aaaaactact agaagatcta ttatttgaag
     1861 caccagatat gcaaatggga gaaataacga tcacagaagc atatgtggat gacaaacttg
     1921 gctcaattgc ggcaaatgaa gatttaagtc gctatatttt ataaaaaatc ggaggacgac
     1981 aatgacagac ttgctaacga aaacccgcaa gataaacaca cttttgcagc aaaagaatac
     2041 ttttgacatg caagcagatt tgccttacga taaaatggct gtcacactag gtggtattct
     2101 tgacagcaac acctatatca taggaagtga cggagtatta ctaggttata atgaacgtca
     2161 cgatgtgaac aatgaacgaa tcaaaacaat gtttgttgag aaacaatttc ctgaatctta
     2221 tactgataca gtggcacagt tgatgaaaac tgaagctaat atcccaatta ctagtgatat
     2281 tactgcattt ccggtagagt caagagagaa atatccattt ggtctgacga ctgtcgttcc
     2341 gatctttgga gctggcgaac gtttgggtac gattatctta tctcgtatgg aacaatcgtt
     2401 tgcagatgac gatctagtct tagcagaata tggagctacg gttgtcggta tgcagatcct
     2461 ttatcagaag tctcgaagca tcgaagcaga tgtacggagt gcaacggctg tccaaatggc
     2521 aatcaacaca ttatcttata gcgaattgaa ggctgtacaa gctatctttg aagcattgga
     2581 tggagatgaa ggtcgtctga ctgcttctaa tattgccgat agtatcggca tcactcgttc
     2641 tgtgatcgtc aatgctttac gaaaattgga atcagctgga atcattgaat ctcgttcgtt
     2701 aggcatgaag ggaacttatt taaaagtatt gaattcccgt tttaaagacg agttagcaaa
     2761 acacagttat tagaagtaag gggactgggg cagatagaaa tgtttgttcc agccttccca
     2821 ttttaaaaag taagtcgaag ttggaggaat ggcaatgact gttattattc aaaatgacca
     2881 gctgatcgcc gaaatcgctg aacacggtgc ggaattgatc agtctgaaat caaaagaaca
     2941 tggtttagag tatatctggc aaggtgatcc agcgtattgg ggcagacatg caccggtttt
     3001 attccctttt gttggacgtt taaaagatga tcagtatgtt tatcaaggga aaacatatca
     3061 aatggggcaa cacggatttg ctcgggatat ggattttgaa gtcattgaac atggacaaga
     3121 aatggctagt ttcatcttaa ggagtacgcc ggagacatta gaaaagtatc catttgattt
     3181 tgaattagtg atcagctatg aattaggagg agacggcatc acggttcatt atcaagtggc
     3241 aaataccggt gaaaaagaaa tgttcttttc tatcggtgga catcctgctt tcaatgtacc
     3301 tcttgaagaa ggattgtcat ttgaagattt ctatctggca ttttctccat tgaaatcaag
     3361 attacgccta ccattgaaag gcccattcat cgatatggat caaaagacgt taggtcagac
     3421 aaatacgaat ctggctttga atcgtgaact ttttgagcaa gatgccttgg tttttgaaac
     3481 aaggggtcta aatgcgtacg ccattcgaag tgaaaaatct tcccatagcg taactttaag
     3541 ctataaagat atgccttatg tagggatctg gtctccttat ccaaaagaag cgcctttcgt
     3601 ttgtatcgaa ccgtggtgcg gaatcgctga cacggtgaac agtagtggaa acttgatgga
     3661 aaaagtaggg atcaatcaac taggtgccca tgaaatcttc aagacaaaat attccatcac
     3721 agtgaagtaa gaggttgtga ccgagaagtt agcagggaac accgtttacc aaggtgtgta
     3781 aaaaagctat cctgtaagta a
//
LOCUS       DAMYXM010000130         3779 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.135, whole genome shotgun sequence.
ACCESSION   DAMYXM010000130 DAMYXM010000000
VERSION     DAMYXM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.135"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(158..322)
                     /locus_tag="PNS21_002744"
     CDS             complement(158..322)
                     /locus_tag="PNS21_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160375.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            617..1945
                     /locus_tag="PNS21_002745"
     CDS             617..1945
                     /locus_tag="PNS21_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HDI5160376.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2147..2980
                     /locus_tag="PNS21_002746"
     CDS             2147..2980
                     /locus_tag="PNS21_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDI5160377.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2996..3733
                     /locus_tag="PNS21_002747"
     CDS             2996..3733
                     /locus_tag="PNS21_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HDI5160378.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
BASE COUNT         1267 a          593 c          789 g         1130 t
ORIGIN      
        1 ttcgagtcct actgccggag taaataaagc ttatgcaaag aaaaagaaat ccaatcactt
       61 gggtttcttt ttcttttatt attatgatga atcaagcttt ctgtttttgt cacgtatgtt
      121 gtaatcttta cgaaagagta gagttggcta ctcataatca accatacttc ccatcatcgc
      181 taaatacgaa ataaatcagc attcctacac caatcaaagc acaagctgca ccaagaaaag
      241 ggatgacatt cccaaagtta tctgtattca taagtatgcc accacccaca aacaaaaagc
      301 cgccagcaat caggtttttc attttattca tctcctttct tacgttatag tatttccttc
      361 tttcaatgag tattttgaaa gctatcttat tgtatgatgt tcttctaggc ctcttcttta
      421 ttatacaggc cattttcgtt atctcataga aatattcctt tttatctgaa aactattgta
      481 taccgtatga catttttctt catgaagttg taagtatatt ctcattcaaa tgaaaatttc
      541 atcatagaag agattgtatt tctaaattct taagagtaaa atacgaagta taacttggat
      601 ttggaaggat gattggatga aagctattac gcctgatatg acaacaaaat ttaacaaaca
      661 gtttcaagca aatatcaaac agcaggcatt gcagcgggca gttgtgaaga acggaattac
      721 tgcttctgct gaaaatgttt cagcacatgt ggagaataca ccggtatttt ctatagattt
      781 ggctacgggg aaagtggcta atcaaatgca atcggggcgt tgctggatgt ttgctgcact
      841 gaacacattc cgtcataaga tgctgaatca attccattta aaagagtttg aattatcaca
      901 aaattacact tttttctggg ataaatatga aaaggcaaat tatttctatg aaaacatcat
      961 tgcaacagga aaagaggcaa tcgatagccg gaaagtagct ttcttactcg ctactcctca
     1021 acaagatggc ggccaatggg atatgatcgt ttcattattc caaaaatatg gcgttgtgcc
     1081 taaatcggtg atgccggaaa gtgcgaatag ttcaaattct cgagatttga ataactactt
     1141 gaacaaatta ctgcgtaaag atgcagtagt gttacggaaa atggttgctc aaggagaaac
     1201 attagacgct attgaagaaa agaaagaaga aatgctagaa agcatttata acttcttagc
     1261 tatctcatta ggaacacctc caaaagaatt cgattttgag taccgcgatg aagaaaagaa
     1321 ctatcatctt gaccgaggac tgacaccaca aattttttat gataaataca tcggtgtaaa
     1381 attagatgat tatgttagcg tgatcaatgc accaacaaaa gacaaaccat tcaatcgttc
     1441 ttatacagta gagatgctag gaaatgtcgt tggcgggaaa gaagtcaaat atctgaatgt
     1501 tgatatggaa actttcaaaa aattagcgat tgctcagctt gaagaaggag aatctgtttg
     1561 gtttggttgt gatgtaggtc aatcttctac tagaactagt ggcatcatgg ccttagatgc
     1621 ttattcaatg gatgatctat ttgatacgga ttttacaatg actaaagctg aacgtttaga
     1681 ttttggagaa agtctgatga cccatgcgat ggtattgacc ggtgttgact tagtagacgg
     1741 gcaatcaaca aaatggaaag tcgaaaatag ctggggcgaa aaagttggaa agaatggctt
     1801 ctttgttatg agtgatgcct ggatggacga atatacgtac caaatcgttg taagaaaaga
     1861 tttattgacg aaagagcaat taaatgcgtt caatgaagaa ccaattgttc tatctccgtg
     1921 ggatccaatg ggtgctttag catagacgag tatttcaatg aagagtatgt agtaaagata
     1981 aaaagagctg gcttaagcca gctcttttta tctttaaagt ccattccctg ttttttatgg
     2041 tccctgtgct ataattctaa ataaaggata aggattggaa ataatccttt tatcattaaa
     2101 aatatgatga cgaagtagaa taaaattggg agtagggggg aaagtcatgg atatccaaac
     2161 tgttttatct gatctgaaat taaatggtaa agtgattcca gttgttggtg gcgatgttaa
     2221 tcagacctat cgtataaaaa ccgaacatcg tgcttatttt ttaaaaatcc atccgaatgt
     2281 aaaaaaagga ttttttgaag cagaagtaga cggattaaaa gaactgtcag cattcgtacg
     2341 tgtaccggat acgtatatgc taggtgaaac aagtgaaggc gcttatttgt taatggaatg
     2401 gatcgagccg ggaaaagggg atcaacgaga tcttgctgca gctttagcta atctccatca
     2461 gcaaacagcg ccacaatttg gttttcgtaa agataactat ttgggtacac ttgttcagaa
     2521 aaattctttt gaagaagatt ggtggacttt tttctttaaa gatcggcttg aatcccagat
     2581 ttctttggca gaagaaacga atcgatggaa tgtacagcgt caagaaaagt atctgcgatt
     2641 taaagaacgt gtattgaaat cggttgaacc aaaaaagatc actccacgcc tcttgcacgg
     2701 agacctgtgg agtggtaatg tgttctttga ccagcaaggg catccagttt ttgtcgaccc
     2761 agctgtttct tatgggaatc gggaacaaga tattgcaatg agccagttat tcggtggttt
     2821 ccgaccggaa tttttagatg cttatcaaac tatatttcca ttagaaaagg gatggaaaga
     2881 ccgactgccg atttaccagt tatattatct tttggcacat ttgaatatgt ttggagaaag
     2941 ttacggttcc caagtagatc aattactaga aaatttttaa gaggagggcg tgtttttggc
     3001 aaaaaaatat accaaaaagc atgaagtttc atattatgag tgtgacatca accaaacgat
     3061 gacatttcct tcgatgttag gagtagttat caaaacgtct gaagatcaaa gcgaccagtt
     3121 gggaagaggc agtgaattcg tcaatagctt tggacttact tgggtcatca cgaattattc
     3181 catggagatt aatcgtctcc ctagagtcgg tgaagtaatc tcagtaacga ctcaagccat
     3241 ggagtacaat aagtttttct gttatcggaa cttctggatc catgatgaag atgggaaaga
     3301 attagtcaaa atagaatctg ttttcgtatt gatggatttt gtgaatcgaa aaatgagcag
     3361 tgttaatgaa gagatcattg ctccttttga gagtgagaag atcaagaaaa tcaagcgtca
     3421 ggaaaaaatt gaaaagattg attcgggctt catgttgcca tatcgtgttc gtttttatga
     3481 tatcgatagt aatcagcatg ttaataatgc gatgtatttt aattggatca ttgacgtatt
     3541 aggatatgac tttttgacta cgcatgttcc agaaaaagta attattcgat ttgataaaga
     3601 agtagagtac gggaatgaga tcgaaagcca ttttgaacaa atccctatcg aaaatggtgt
     3661 caaaacaaaa catgaaatcc gaatgcaaga acagctgtat tgtgaagcaa atattatatg
     3721 gaaaaataaa taaaaaagag gttgtgacag aagggggcaa cttcaagaaa taagaagaa
//
LOCUS       DAMYXM010000131         3692 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.136, whole genome shotgun sequence.
ACCESSION   DAMYXM010000131 DAMYXM010000000
VERSION     DAMYXM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.136"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..504
                     /locus_tag="PNS21_002748"
     CDS             <1..504
                     /locus_tag="PNS21_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDI5160379.1"
                     /translation="SEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLTYKEGSAQ
                     VIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAKTPLQSEK
                     NIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLLLVLATFS
                     YAVLRYI"
     gene            531..995
                     /locus_tag="PNS21_002749"
     CDS             531..995
                     /locus_tag="PNS21_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160380.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            992..1234
                     /locus_tag="PNS21_002750"
     CDS             992..1234
                     /locus_tag="PNS21_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HDI5160381.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            1252..1554
                     /locus_tag="PNS21_002751"
     CDS             1252..1554
                     /locus_tag="PNS21_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160382.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            1624..3687
                     /locus_tag="PNS21_002752"
     CDS             1624..3687
                     /locus_tag="PNS21_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160383.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
BASE COUNT         1452 a          560 c          763 g          917 t
ORIGIN      
        1 tctgaagtac aatatctagt cacacaaaaa tggcacacaa aaggcgtaga cgctgcgtca
       61 gatcactaca aacaatttag tattcaagat ccaattgaag ctcgtttgac atataaagaa
      121 ggttctgcgc aagtaataga taagagtaaa ggaaaagata ttacttctga aggcacacta
      181 acctatgata gcaattcaag aacgctgaag tgggaagctt ccgctgactt cttaagtaac
      241 aatcttttag atggacgaga aattcagctg atatttacag ctaaaactcc attacaatca
      301 gaaaaaaata ttgataacca agccgtagta gcagtagata atgtttctaa taaaacaaat
      361 gttgtgacaa ttggggttga tcctaacttg ccacaggtca ttgttcctaa aacaggttct
      421 acacatttag taacgatttc agcggtcagc ttactattac ttgtgttagc aacttttagt
      481 tatgcggttc tgagatacat ttaaaataaa aaaacgaaag aatgaataaa atgaaaaagt
      541 taataatagt agttacttcc ttgattgcca tgattgttat tttagtcgga tgtagtaatg
      601 aaaaaaataa acaaactaat caagataatg gcgtattgaa atcaggaact atgtggaaag
      661 aagaagtagg tggtcttgtt tataatttga aaattataga cgagactacc tgggaatact
      721 ctgagagtgt atggcatcca gatcctgtac aaataacagt caagaggcaa aaagactata
      781 aaggtttaga aagatacaaa atagtagatt cagctggtgt tagggaattt ataaacaaat
      841 ctgattcttt atttattgtt gttccctatg agaagaatgg cgtaaaaaaa ataatttttc
      901 ttggatctag caaggatgaa aagcgaacga aagagaaatt gattcacgat ggatcacaat
      961 ccaataaata taagttacaa aagactagcg agtgattgct agtcttttta tttgtcctga
     1021 aggaggaatc acagtggcaa aagctaaaac gccatttgaa aaagccgcgt ctaaaatctt
     1081 agaacgccgc ggtgtatctg aaacacagtg gaaacaagaa gtccttgaaa aagcacaatt
     1141 tgacctatta cggggaaaag acaaggactt agaaggatat atctatcaac gtgaacgtga
     1201 aaagcttgtt ttcgatgaaa ttattaaaca ctaaaaggag cgaacaattt tatgtttgaa
     1261 tcaattacaa tgaatttttt aacacaaccg aaagtagtag ctgcggttga tatttttaga
     1321 gcattacaaa ctgccgcaac tagtttaaaa aacaatctga tgatttttgc tgctgctgcg
     1381 gccgttgctt ccattgttat tgggggtctt ttcttcctat ttggacgaag aggagctgaa
     1441 aatgggaaga tgcacgtagg ctatgtattg atgggggtag gagttatttt agcagcagct
     1501 tctattgtgg tttcgttatt tggattgttt ggacaaacac ctcaaggatt ataagaaagt
     1561 aaaaaagtag gagtaagaag tagtcgatac tttttactcc ttttttatga aaggagaata
     1621 ctcatgtact ttacgattgc aggcaatacc aaaacagata aagagttaat aaaaaaagca
     1681 acgaaggaag taggaaagcg tacttctttt ttaccgaaaa caattaaata ttctgtcatt
     1741 gaaacggaag aacagctaca tgtgttaggg aaagtactgg aacacgaaag tcgatcttct
     1801 gttaagttga ctctcacgtt acatgacaca gaaggaaaaa tagagaaagc tggtacagaa
     1861 tacgtttttg gcccactgga ttatcaaatt tcagaaggtt tctatcctac attgatggat
     1921 ttaatccgcc ttgatgtgtt taaagaaacg gatgaaacta tgagtgtgga agagtcttat
     1981 cgaaacgaaa aacttttaca agttttagaa gaagagttag agagcgacca aagaaaagtg
     2041 aatcaaggga gtgaagtcga agtaaatgaa gaagtaccat gggaacgacc aatatttcca
     2101 aagaaggagt caaaagaaaa agagacagtc tatgttgacg aacaagctga tattccgact
     2161 cagaaatcga tatttgagga agagaacgat gtgatgggtc aattggaggt agatcccttt
     2221 gaagaaacaa tggaggatga agagacggcc gaagtagaag ttgatccagt tgaagcgaat
     2281 gaagagtcaa catggactga aggggattct caggaatata ttgaaccact tatcagtgaa
     2341 gaagtagaaa aaaacgaaat agagatacaa aaggcaactg tatttgatta tattttggat
     2401 caatatccgc cagagcatga atggatgaaa gaaaaaaaca aattattttt aaatgagctt
     2461 tattcgaaat atcaattacc ccaaacctat caagcatttt tagagagtaa agaagaatta
     2521 atctcacagg gtaaaggcac acttttagaa cagttagagt ctgtccaatc aacaaattgg
     2581 catgagcttg cacaagaaga tttattagcc acctttgaag aacgtcaaca agaaacagat
     2641 caagaaatta atgagtatgc gacggaacag tcgtctaatt gggagaaagc aaaagaggaa
     2701 ctaaataaag aagaacagcg agtgattgaa gaagaagtac aaaaaattaa agagcgatac
     2761 gaaaagaaac gcgacaatgc gtattccttg tgtgtagcaa agatcaataa ttttactcag
     2821 acgaatcaag aagcgttatc gaaagaaaaa gaaaacttgt taaaagaacg tattaaagaa
     2881 cgaaaagaag aattttaccg aggtttgcgt acagataagc taaagattag caaagaatta
     2941 aatgaacaac tgaataagct atatcaaaca acgactgaag ttcttatgga aaaatacgaa
     3001 aagatccagc aagaattaca agtgcaaatt ccaaaatgga aaaaagagta tgaaattcaa
     3061 caacaagaat tgaaagaaca agaagaaaaa caacggcaaa aagaaaaaga gcgcgaacaa
     3121 ttagaactaa aaaaacgtga acaggatctt gcggaacaac aaatggcaat agaaaaacaa
     3181 cggttaaacc aagaaaagga aaaagaagca gctgctcgta aggaacgtca agaacaaatg
     3241 gatacattgc gacaagctca gttaaattat ctagcacaac ctcaaccaac gccttcagta
     3301 gctgctgttc aaaccccaat gaacaaccaa acaaatctaa ctccttcacc acagcgttcc
     3361 tttaacggat ggatggtggg atgtattgca agtttaagct tgcttctagg tggaggaaca
     3421 gtatttgctt ttaatcatat caatcaatct tcacaggctg attctgtggc cacaacgacg
     3481 tcaatcaagg aagaagccca agaacaagca agaaaagagt atgaacaaca attagaagaa
     3541 ttattgaatc gtaccaatca gtcaacaaca gaatcaagta tggaaaatag caaagagagt
     3601 gatcctagta aggctaaaga aggatcaagc gattcaactg tacaatcaaa cacttccgaa
     3661 agttcagaat caaatgaggt gaaataatgc gt
//
LOCUS       DAMYXM010000132         3624 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.137, whole genome shotgun sequence.
ACCESSION   DAMYXM010000132 DAMYXM010000000
VERSION     DAMYXM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.137"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            746..829
                     /locus_tag="PNS21_002753"
     CDS             746..829
                     /locus_tag="PNS21_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HDI5160384.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            954..1691
                     /gene="erm(B)"
                     /locus_tag="PNS21_002754"
     CDS             954..1691
                     /gene="erm(B)"
                     /locus_tag="PNS21_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDI5160385.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1954..2070)
                     /locus_tag="PNS21_002755"
     CDS             complement(1954..2070)
                     /locus_tag="PNS21_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta-toxin"
                     /protein_id="HDI5160386.1"
                     /translation="MVQNQHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI"
     gene            2333..2845
                     /locus_tag="PNS21_002756"
                     /pseudo
     CDS             2333..2845
                     /locus_tag="PNS21_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            2894..3574
                     /locus_tag="PNS21_002757"
     CDS             2894..3574
                     /locus_tag="PNS21_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDI5160387.1"
                     /translation="MNHFKGKQFQKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKILYHLWKKKNRQSFYSWKMDETYIKIKGHWHYLYRAIDSDGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGQPRVIVTDKAPSISSAFRKLQINGLYTKTEHRTVK
                     YLNNLIEQDHRPIKRRNKFYRSLRTASTTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGIPA"
BASE COUNT         1197 a          597 c          687 g         1143 t
ORIGIN      
        1 ttgacaacca tttttgttag gcgtttgagg aagtgaagct gttttcctca tgttttaaaa
       61 gctagaaata aattacagtg tacgctcctt tcttttttcg ttttgttggt tcattttcgc
      121 cagtgtgttc gtgtcgtttg ctgtgacgag tacgcaacat agacacaaag aaaacacaag
      181 ggcgagcttg ctcgtttata tacccttgtg ttttcgactg gatatgttaa ggtaacaaac
      241 agtaaatgac acgttacaga ctgcaaaatg taccaactga aaaaagaaag aataccgttg
      301 tgtgttttat ttctcgatca gcttttaaaa catggggtcc cgagcgccta cgaggaattt
      361 gtatcgataa gaaatagatt taaaaatttc gctgttattt tgtacattta acttgacggt
      421 gacatctctc tattgtgagt tattagtggt acagttttca accgttttaa ttataaaaaa
      481 gtggtgcatt tttaaattgg cacaaacagg taacggttat tgcaggtgta tttcttatct
      541 atgggtttaa catggatttt atcattaaaa tcatgagtat tgtccgagag tgattggtct
      601 tgcgtatggt taaccctaaa gttatggaaa taagacttag aagcaaactt aagagtgtgt
      661 tgatagtgca ttaaaaattt tgtataatag gaattgaagt taaattagat gctaaaaatt
      721 tgtaattaag aaggagggat tcgtcatgtt ggtattccaa atgcgttatg tagataaaac
      781 atctactgtt ttgaaacaga ctaaaaacag tgattacgca gataaataaa tacgttagat
      841 taattcctac cagtgactaa tcttatgact ttttaaacag ataactaaaa ttacaaacaa
      901 atcgtttaac ttctgtattt gtttatagat gtaatcactt caggagtgat tacatgaaca
      961 aaaatataaa atattctcaa aactttttaa cgagtgaaaa agtactcaac caaataataa
     1021 aacaattgaa tttaaaagaa accgataccg tttacgaaat tggaacaggt aaagggcatt
     1081 taacgacgaa actggctaaa ataagtaaac aggtaacgtc tattgaatta gacagtcatc
     1141 tattcaactt atcgtcagaa aaattaaaac tgaatactcg tgtcacttta attcaccaag
     1201 atattctaca gtttcaattc cctaacaaac agaggtataa aattgttggg agtattcctt
     1261 accatttaag cacacaaatt attaaaaaag tggtttttga aagccatgcg tctgacatct
     1321 atctgattgt tgaagaagga ttctacaagc gtaccttgga tattcaccga acactagggt
     1381 tgctcttgca cactcaagtc tcgattcagc aattgcttaa gctgccagcg gaatgctttc
     1441 atcctaaacc aaaagtaaac agtgtcttaa taaaacttac ccgccatacc acagatgttc
     1501 cagataaata ttggaagcta tatacgtact ttgtttcaaa atgggtcaat cgagaatatc
     1561 gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa acacgccaaa gtaaacaatt
     1621 taagtaccgt tacttatgag caagtattgt ctatttttaa tagttatcta ttatttaacg
     1681 ggaggaaata attctatgag tcgcttttgt aaatttggaa agttacacgt tactaaaggg
     1741 aatgtagata aattattagg tatagggcac ctctaataac taccaattaa ttcacctcaa
     1801 aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt tcttatcgat acaaattcct
     1861 cgtaggcgct cgggacccca acatgaggaa atgtttttat aaaaaaagag tagttaccgt
     1921 tttttggtaa ctactctttt ccttttttaa ggtttaaata cctggaagtt taggtgtttt
     1981 gggtattggt ggtgtaggtg gctgcaagct ttccaatttt tgttgaattg ctttaaattc
     2041 aggggtttct tggtgttgat tttgaaccat tttttgctct attctctcca aagtcggttg
     2101 aatttctttc cctgatactt tacgatttaa ttctctttct aaggtctctt tcggactaat
     2161 ggaaggcgtt tctaagcttg aatagagttt cactccttct ctattgtaaa gccttatatc
     2221 gctaaataag cccgttttat gaagggtttc taaattggtc ggtaagtttt tgacaacaat
     2281 atcatgcgct tgttttggtg gtgccctagc tgtcattgga tcatctgcat acaataagat
     2341 ccatgaaagg ccatctgctg ccaatgagcg ccttgagatt ggtaattggg aagctgatac
     2401 tgtagccggt aaaacaggta gggctcgtat tgtaactcta gtcgatcgta gatctcgttt
     2461 ctccctactt ggtaaggttg aaaagaaaat ctctaaaaat gtggttgatt gtatgatcaa
     2521 tctactaaga aatgtaggag aagataaatg taaaaccgtt acacccgata gaggaaaaga
     2581 attctcccaa catagtcgaa taacgctaga attaaatggc actaaagtgt attttccaga
     2641 tccacatgct ccatggcaac gtggtacaaa cgaaaatact aatggtttat tacgagaata
     2701 ttcacccaaa ggtgaagata tttcaggtat tttcgatagt caaattcaag aatgggcgaa
     2761 taaattaaac acgcgaccac ggaaatgtct aaattggaaa accccttatg aagtattttt
     2821 ggttctgttg caaagttccc cataactcct gatttattgt aaaatgtaag aaaacgagag
     2881 tgagggtaga cagatgaatc atttcaaagg gaaacaattt caaaaagatg tgattattgt
     2941 cgctgttggt tactacctgc gttacaatct gagctatcgt gaagttcagg aattactgta
     3001 tgatcgcgga ataaatgttt gtcacactac gatttatcgt tgggttcaag agtacagtaa
     3061 aatcctctat catctttgga agaagaaaaa tagacagtcc ttctactcgt ggaaaatgga
     3121 cgagacctat atcaaaataa aaggccattg gcattatctc tatcgtgcga ttgattctga
     3181 tggattaact ttagacattt ggttacgaaa gaaacgggat actcaggcgg cttatgcttt
     3241 cttaaaacgg ctccataaac aatttggaca acctagagtg attgtaaccg ataaagcacc
     3301 atcgattagc tctgcattca gaaagctaca gattaacggt ttatatacca agacagaaca
     3361 tcggacagtt aagtatctca ataatttaat tgagcaggac catcgtccaa ttaagcgccg
     3421 taataaattt tatcgaagtt tacgaactgc ctcaaccacg attaagggca tggagactat
     3481 tcgaggaata tacaaaaaga accgaagaaa cggaacgctc ttcggatttt cggtgtcgac
     3541 tgaaattaaa gttctgatgg ggatcccagc atagtcaaaa tattaaacta ggacttctat
     3601 actcttttgg ggaactttgc aaca
//
LOCUS       DAMYXM010000133         3491 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.138, whole genome shotgun sequence.
ACCESSION   DAMYXM010000133 DAMYXM010000000
VERSION     DAMYXM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.138"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..2233)
                     /locus_tag="PNS21_002758"
     CDS             complement(<1..2233)
                     /locus_tag="PNS21_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160388.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2905..3173)
                     /locus_tag="PNS21_002759"
                     /pseudo
     CDS             complement(2905..3173)
                     /locus_tag="PNS21_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          984 a          772 c          549 g         1186 t
ORIGIN      
        1 aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct ccttgaagga
       61 cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca aggatgtttt
      121 caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca tgtaccgtcg
      181 catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta cctacaggaa
      241 gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc acttgtgaat
      301 cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca tcagttttaa
      361 ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc aatcgattgt
      421 cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac gcctctacat
      481 tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc gcactgaaac
      541 gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat agaaatgaca
      601 caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt gttccagaaa
      661 acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata acatttttgt
      721 ccacactttt tcttaaagag attgccggaa gattactttc agggattctc aagcggtagt
      781 ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa aacccatcct
      841 cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat tcaataaaat
      901 acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt tccttattaa
      961 aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt gcttcacctg
     1021 gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt ccagtggaac
     1081 tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg gtaaccacta
     1141 caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta ctgttggtca
     1201 cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt tctctagagc
     1261 catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt gaaaattccg
     1321 tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt ggctcagtac
     1381 cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac tgagcggctt
     1441 catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct tcttggtaaa
     1501 atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac tcaattaagc
     1561 caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga atatcgccag
     1621 cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa gtggggcgct
     1681 cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc tgagattcct
     1741 gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag ctttcttctg
     1801 gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca cttgtgcttt
     1861 cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg cttcttgcgg
     1921 tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg taatcattga
     1981 gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat gcatttagca
     2041 ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc tcctcttgat
     2101 caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct agaaccgtta
     2161 tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac agtttatgaa
     2221 atttgttcct cactattact cccccttata attttgacta aacaacaagg tacccgataa
     2281 ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta ttttcggttc
     2341 aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt gtcttctgtt
     2401 gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc ataattatga
     2461 tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta atagttattt
     2521 tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta aatcgttgat
     2581 accaaggtca gactctagaa tttcatcagt gagtccctgt attggttaac cttcatcaat
     2641 cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga atacttggga
     2701 ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc aactaaacgc
     2761 atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta ctaataaact
     2821 gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc tttttgctct
     2881 ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact ccttaatcat
     2941 cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag tgacaatctg
     3001 tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta ccatatattg
     3061 caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta tagaataact
     3121 accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc ttttgttgta
     3181 gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg tattgctatt
     3241 tttgctaact ggcgtatttg caactatttt tcccttctat tcttccttca ttgattcccc
     3301 tctaaaatta ctatccataa aaaattgcat aaatgacaat agtatttata ttagtcatac
     3361 tgctttttaa cacagcaaat tccagaaaaa cattcgttac atatttgtga atttttccaa
     3421 aaaataacat ttttataaaa cgacgaaaaa taaatcacta ccagtcaact tctataatat
     3481 taccaaataa c
//
LOCUS       DAMYXM010000134         3483 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.139, whole genome shotgun sequence.
ACCESSION   DAMYXM010000134 DAMYXM010000000
VERSION     DAMYXM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.139"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(781..1737)
                     /locus_tag="PNS21_002760"
     CDS             complement(781..1737)
                     /locus_tag="PNS21_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160389.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(1846..2091)
                     /locus_tag="PNS21_002761"
                     /pseudo
     CDS             complement(1846..2091)
                     /locus_tag="PNS21_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2236..2658)
                     /locus_tag="PNS21_002762"
     CDS             complement(2236..2658)
                     /locus_tag="PNS21_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160390.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(2838..3209)
                     /locus_tag="PNS21_002763"
     CDS             complement(2838..3209)
                     /locus_tag="PNS21_002763"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDI5160391.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
BASE COUNT         1069 a          740 c          561 g         1113 t
ORIGIN      
        1 actacttttt ttatgtaaag ttctgtcaaa tatagtcact ttatattact ttctcttgag
       61 taaaaaaaca tgtgaggtcg taaacaaaga ttgaactatc tacattcata ctctataatc
      121 tgtttatttg cagactgata atagcaaaag aaaacttata atcaaataat atcaacattt
      181 gatcacataa tttgagattg aatgctgatt tacctatgtt ttacgtaatg gtctttagtt
      241 atttttgctc ataaaatgca taaaacacgc gtttttatgt gtgcttgaaa tttcttggct
      301 ctataatttc taagactgat aaaaatattc tatcggttct ttttgcaaat aaaaaaacat
      361 atagaagctc cattagattg aatcaccaca acacaatcaa aggagcattc cgtatgcttg
      421 attttcattc tataaaacta cttttaggaa tcaaagaaaa tcacaacggt tcaaactaaa
      481 agcaagaata tccgaagtac cattgtttca tcgatcatga gaaaagcatt cacctctgtc
      541 tgcagatgtt cttatatcgc tctgtcagtc ctattaataa agtactgttt tgttttcaaa
      601 aaaactatta cgagtacttt ttaagagaat gccttgcaaa tattcatcac acaccgttat
      661 ataagtcaaa tagtttcttt cagtcgtcaa gaaaagattc tcctgtcctt ggcattatca
      721 gttaagtagc aacttaattc tcggacttgt tctactctag caaaatttat tttcatcaac
      781 ttacgtccaa atgttttttt gaataattac ttcatcgcgg gtctggaaga cttgcctcat
      841 tcgataagtc actagctttt ggcgtccttc aggatttcta gtatacacca actgacattt
      901 gggcagcgac gattgattta attcgtttag aaaaaggtta gcgatttgct tatttcggcc
      961 aaagcgttct ggtaccgtca gaactatctt tttcccttcg attaaatacc gcggattctc
     1021 cagcggtgct aaaagctccc tcatagcttc agtaaaaagc tgtccttctc gatagccagc
     1081 attctcaaga tagcatctaa attcctgctg atcagccgct acgattactt ttctatcact
     1141 aacttcacca aggcgctgca acgtcgtttg aacgcatatg gccaagcttt ttttgcgggc
     1201 acattccttt tcccatagct tttgtcgcct acgaatttgg aaatattcca gcaagttacc
     1261 tttaaatata gctgaccaga agcttagagg ctctatattt tttactacta tcggcaatga
     1321 agtttcttgt ttttgcttta cctgatcgac tttcctaacg acgattggca tagataggtg
     1381 ccagccatca gctaaagcag tactccattg ttctttcaac cgacttcgtt cacaagaaaa
     1441 atagaacatc gactgagtat aagtttccat cgtggtggtc gtatattcca gtggtaaagc
     1501 aaagcgatcg ccaccactct caatttttgg tgaagattca aaggtcagac ctgaaatatt
     1561 gcccaaacgc cgtagcattc ttttcaaatc aggctgttca gacagaggaa ctcccggtaa
     1621 tccaatcgct aaatgccaaa tgttcgttac tttattttgt gcacctaggc gtaagccccg
     1681 ccctcgtagt tgttgtgttt gcacaaaagt ggaagcttga ttagccaaga tcaacagatt
     1741 aattgcagga caatcccagc cttctcctag caaactaacg gttcctacca aaatattcaa
     1801 cttcttttcg gttaataggc gagtaactgc ttgcatcaat gcttggcggt ttttgtcatc
     1861 caccgtcaaa taaaaataac ccgacatata aggatgttca ttcatacccg tcgctagttc
     1921 tgccagctca acagttacta gcattttcgg taacagtaag aactctccac agatggcacc
     1981 gatttgctga gtgttaccta atagacgaac aacttcatca aacaaaggaa ccacgccata
     2041 agctaaggta ccatcactgc ctgctaatac ctccttgcaa atacgatcca tcaaaatgac
     2101 cgctgtcagt tcttgcttca gctgaacttc ttcttttaac aaaatttgac aaactgcttg
     2161 ctttttctca ggaatcgcct gactaccaac agtgtctgga tactctggaa acaaatgcag
     2221 cgactcatca aacaatcatc cagctttttt caattgataa aaaataggca attccggttg
     2281 atcttcaaac atctgctgat aaatagtgat cagctctttt ttacctaaag atggaactga
     2341 agctttttta ccttgctgct tagaactgcc taatagctgc caatgttcgg gtatcaattc
     2401 atagttatga tgattcaagt aaattaatgc actagtgaac agctcaaaat tttcataaat
     2461 ttccttcgaa tgctcaagcg gatgctgaac aaattctaaa gaaagcaaat aatccaccgt
     2521 ctcttggctt tccattaacg cttccaccag ctcattttgt ttctctaaaa aggtaaaata
     2581 atgctgagta gtttcctgat tagccggaat caaatataga aaatcctgat gaggacatag
     2641 aacctcctcc tttaccaact cgggaatgct aatttcttcg tcaatcaggc cattttaact
     2701 caatatattg cttccactcc gtctgagagc tatcaatcgg aggcgtggct gtcaaggaaa
     2761 tcgtcaacag attcggttgt tttttcttca agtcaatcaa caattcaccc cagctttttt
     2821 ttaaatgatg agcttcatca aaaaactaac agctccagct gatcaccata agttggctcc
     2881 tccttcagtc ggttgtaaat ggtctgatac gtttccactg taatatgact aggatgttca
     2941 atcgccccag aaagatagca actatctgct ggatttacta agaaatattc ctcgattgtt
     3001 gttaaccact gtttcttcaa tattagggaa ggaaccaaaa ttaaggtttt acaatccaaa
     3061 cgtcggatca tctcaattcc taaaaaggtc ttgccagccc ctggtggagc cactagatgg
     3121 actgaattgt tatttaaata agtagcaaag ttagtcaata tacgcttctg atagctacgc
     3181 cactgaattt tttcatcaga tagccacatc ccaggtcccc ctctcacgta ttttctctat
     3241 cataatctaa atgaatacag caaaaaaaag atttcttcaa gaatatgtgt ttttatagta
     3301 atattttact tactctaaac tcaaatgaaa aggtaaaaaa actggtagta caatctttta
     3361 aattctcatt gttttgtacg ttctttacta tcaaaaaaac aaaaaaaacg cgtttctacg
     3421 cgtgaccaaa atttttagct ctataatttc taagactgat agaaaatcat tctgtttttg
     3481 aag
//
LOCUS       DAMYXM010000135         3294 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.140, whole genome shotgun sequence.
ACCESSION   DAMYXM010000135 DAMYXM010000000
VERSION     DAMYXM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.140"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            39..590
                     /locus_tag="PNS21_002764"
     CDS             39..590
                     /locus_tag="PNS21_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HDI5160392.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            1266..1520
                     /locus_tag="PNS21_002765"
                     /pseudo
     CDS             1266..1520
                     /locus_tag="PNS21_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1590..2102
                     /locus_tag="PNS21_002766"
     CDS             1590..2102
                     /locus_tag="PNS21_002766"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI5160393.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            2099..3136
                     /locus_tag="PNS21_002767"
     CDS             2099..3136
                     /locus_tag="PNS21_002767"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDI5160394.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1214 a          555 c          654 g          871 t
ORIGIN      
        1 agagcgagca agacaggaac gagagaacaa acatactcat gaaaagacta gaggcttcga
       61 cctcgaactt taataataag ttagatgttt ttgaagaaaa gactaagcat gcaagattaa
      121 actttgaaac aactgcaaag cactatatta aacgacttga tgaagataat ttgaaattag
      181 attttcaaca agctattcaa gatgaattaa gcgatactaa agacgaaata cgtgaagtga
      241 cgaaacaagc gagagaagaa acaaaagagt ataaagaaat cttagaaagt aaaataaaag
      301 accataacaa agtggttgat aaatcgaata cagcgctaaa agtgatgaca aaaggcgtta
      361 caaatatatt ttttgtgcta attatttttg tacttgtaat gcttgtgaca ggtccgatag
      421 gtcatttctt tggtattgaa catttatata gctttattaa tggttttatt gatgaccacg
      481 aaagtgcatg gcgatatttg atgttgatac tttatgcagt accctatgta ttcttcgcta
      541 tcattctgtg gctcatagca ttgtttttta gagtatttaa tgacctttag gtagatttat
      601 atataaaaag ggctaaatac gcaaaatacc cccttaaaat gcaaaataaa tgcaaataag
      661 ggggtaactc cttatgaaaa aacaactaat agtattataa cacgttttta tgccgagaaa
      721 acttattggt tggaatgggc tatgtgttag ctaacttgtt agcgagttgg ttggacttga
      781 attgggatta atcccaagaa agtaccaact caacaacaca taaagccctg taggttccga
      841 ccaataagga aattggaata aagcaacgaa aggagttgag taaaatgagt gagaaaacga
      901 agaaaatcat tcgagtatca ttagcttttt cacaagtagt agtgcaatca ttgatagttt
      961 attatactta ccagcaatat aaattatcta aagagaatta agttttgatt atgtaataag
     1021 ttttggtact aaaaaagact tgatctaatt agaccaagtc ttttgttagt gttatattaa
     1081 taacaaaata aaaataagaa gtcgctcact ccccgaccaa agtttgtgag caaaataaac
     1141 agtcgatgtt tgtttcttaa ttgtatatct tgatattaaa cgatttaaga ttattcttca
     1201 agatatatat tcgcaaatgt aattggaaaa gcgacttctt aaacaaaatt aaggagtcgc
     1261 tttttatggc aaaaataacg aaaaatgagc aagaaaattt ggtggaaaat tacacaaaaa
     1321 aaccggcgta ctctaatagc cggttagacg cacatttcgt gtgcacttct aatccgaaat
     1381 tgagctttga cgctatgacg atagtcggaa accttaataa aaacagcgct aaaaagctat
     1441 ccgattttat gagtttagat ccacaaatta gactttggga tatacttcaa acaaaattta
     1501 aagctaaagc actacaagac cccctatgtt aggctagttg tcgtatagga taataagtta
     1561 taataaagac tatacggagg atgattacca tgacaaataa aaacaatgaa cgttactcta
     1621 ccgaactgaa agcgacagcc atcaagaaaa tgatgccacc cgaaaatagg gggattcgcc
     1681 aattaagtca aactttgggc attccagaag cgactctttg ttcttggcgt aaaaaagcta
     1741 gaattcaagg gaatactaca cccggaacga aaaaaaatgt cgaaaaatgg tcaagtgaag
     1801 ataaattttt aattgtgatg gaatcattct caatgaacca agtagaactt gcggaatatt
     1861 gccgaaagaa gggcttattt aaagaacaaa ttgaggcttg gaagaaaacg tgcttatctg
     1921 cgaatgaaca ggaagaaaat cgaacgagag aacttgcaac tgaactaaaa gaagagaaaa
     1981 aacaagcgcg tcaattagag aaagaattac gtcataagga aaaagcatta gcggaagccg
     2041 ctgctctctt attactaaga aaaaaagcac acgcgatttg gggggaccaa gaggaagaat
     2101 gatttcatct tcagatcgca aacaagctgt tgaacttatt caagaagcca atcgcaatgg
     2161 ggcccgtctc acctatgcct gtaatgagct gaatattagc gttcgaacct acgaacgatg
     2221 gacgcgtgaa ggtacgatag cgcatgatca acgccctttg gcgaaacgtc ctgtacccaa
     2281 aaataaactg acggatcaag aacgcgaaaa aataatagaa accgtttcaa aaaaagaatt
     2341 tatgaatttg ccacctagcc aaattgtacc aaaacttgcg gattgctcga tttatattgc
     2401 atcagagtct agcttctatc gtgtattgcg agaaaaaaat atgcaacatc accgtggccg
     2461 aagtcaagtt tcacaaaaac gcataccgcc aagtcacctt gcgacgaagc caaatgaagt
     2521 ttggacttgg gatattactt ggctaaaagg tccaataaaa ggactctttt accggttata
     2581 tctcatcatt gatttattta gccgtaaaat ggtaggctgg gagatttggg aatcagaaga
     2641 agctcgtcat gccgaaaaac ttattaaaca gacggtgctt aaggaacgta tccaaggaca
     2701 gccgttggtt ttacactccg ataatggcag ccctatgaaa gccgcgactt ttcaagtctt
     2761 actcgaaaaa ttaggaattc aaagttctta ttcaagacca cgagtgagta atgacaatcc
     2821 ttattcggaa gcggctttcc gaacgttaaa atatcgacca gaatatccca ataacggctt
     2881 taaaacaata gatgatgctc gaaaatggac gaaagagttt gtcgattggt ataacgattc
     2941 tcatttgcat agtgggattc aatttgtgtc tcctgcagcg tgtcatgaag gacgacatct
     3001 cgctatttta gagaaacgaa agcaggttta cgaggacgct aaaaagcgcc atcctgaacg
     3061 ctggtcaaaa ggtgttcgaa attggactcc acatcaatcg gtggcgttga atcctttaaa
     3121 agaaacagaa agttaacagg agcataatct ttcaagatta tcaaataacc ctcgtcaccc
     3181 aataggggaa aaagttattc ttcccctatt ggataacgag gccaaccgac catagggagg
     3241 ttctgctttt tgataggtgg aaaataaaaa tacgacaact atattgacaa acac
//
LOCUS       DAMYXM010000136         3283 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.141, whole genome shotgun sequence.
ACCESSION   DAMYXM010000136 DAMYXM010000000
VERSION     DAMYXM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.141"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            3..587
                     /locus_tag="PNS21_002768"
     CDS             3..587
                     /locus_tag="PNS21_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDI5160395.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(664..1857)
                     /locus_tag="PNS21_002769"
     CDS             complement(664..1857)
                     /locus_tag="PNS21_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDI5160396.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            1921..2301
                     /locus_tag="PNS21_002770"
     CDS             1921..2301
                     /locus_tag="PNS21_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDI5160397.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            2375..2737
                     /locus_tag="PNS21_002771"
     CDS             2375..2737
                     /locus_tag="PNS21_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160398.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         1115 a          538 c          622 g         1008 t
ORIGIN      
        1 atatggcatt cccacaacta gatttaaaaa gtgaaaaagg accaaaagct gttatcaaaa
       61 caaaccgtgg tgacattact gttcaattat tccctgaact agcaccgaag acggttcaaa
      121 atttcattga actttccaaa aaaggttatt atgatggcgt gatttttcac cgcgttattc
      181 ctgatttcat gatccaaggc ggcgatccaa ctggaacagg aatgggtggc gaaagtattt
      241 atggcgaaag ttttgaagat gaattcagtc gtgaactgtt caaccttcgc ggagctttat
      301 ctatggcaaa ttcaggtcca aacacaaacg gaagccaatt ctttattgta aataacacaa
      361 atgtgccagc taatatgctt ggacaactag aaggtgctgg attcccatca gaaatcattg
      421 aagcgtataa aggtggcgga actccttggc tagatttccg acatacagtc tttggacatg
      481 tactagaagg gatggacaca gtagatgaaa tcgcaaatgt acaacgtggt ccacaagacc
      541 gacctgtaca tgatgtagtg attgaaacaa ttgaaatttc agaatagttt ggtatggtac
      601 ggagtaatta tgaaaaagga cgaaacctag taaggtctcg tccttttatt ttgaataaat
      661 gatttaatcg attgtctcta aagcatactt gattcgatta agtccttcca gtaaagtttc
      721 ttctggacaa gcaatattca agcgcatatg cccatgaccg gaaggaccaa acgtgatgcc
      781 aggatttaat acgacttttc ctttccgaac aagagtgttc tccaattctt ggtcgtctga
      841 aatataggcg gtaaaatcta accacattaa gtaagttcct tcagggttca ttattctcac
      901 tttcggaaga tactgttcaa aaaactgatt aactgtttga acattttttt ctagataagg
      961 aatcagttgg cttaaccatt ctccacccgt ctcataagcc gcttgggttc caatcagtcc
     1021 aaaagtgttg atttcgtgct gttggttcat cactaaatgt ttttcaaaat cagctttcat
     1081 ctctggattt ttgataaata ccattgaatt tttgattgca gctagattga aagtcttggt
     1141 tgcggaagta aaagcaacta gtagatcatc taaattagga tcaatcgtta gcatggaagt
     1201 aaatgtatga tcgaataaag ttaaatcttg atggatctcg tcactaaaca gaatcacttg
     1261 atgttttaaa cacaattcgc ttaactgttt caattcttct ttgctccaaa cacgccctcc
     1321 aggattgtga ggattgcata aaatcattgc ttttacattg ttttcttcga tttttttctc
     1381 catgtcagac aagtccatga tgaaatgatt atttttttct agcaaagagc ttcgaacgat
     1441 gtgacgttga tttgtttcta taatagagga aaatggagga taaactgggt catgaatcaa
     1501 aatagaatcg cctggttttg taaaagtttg tactgctgca gctaaactgg ttaacacacc
     1561 acttgtaaaa acgatatttt ctttcgtcaa ctccacagca tggtgttggc gttcccaatg
     1621 aatgatagcc tgatatagtt tatcagacag agtggcataa ccaaagattc catgtttgat
     1681 gtattcacta aacgctttag ctaccccacc gggagacaag aagtccatat ctgctaccca
     1741 caaaggaagt aagtcatcca tttgatatgt ctcagcaatt gcgtcccatt ttacactttc
     1801 tgtatctttt cttgaaatct gtttatcaaa ttcgatcatc ttaagacctc cagtcatttt
     1861 cgtcgcttct ttttttgagc tatttacagt ataatgataa atgcgaagag agggaagtag
     1921 atgacttata aaattggaca aataattgaa ggaaaagtga cagggataca gccttatggc
     1981 gcattcgttt ctcttgataa cgagacgcaa ggacttattc atgtttcaga agtacaatcc
     2041 ggttatacga aaaatatcca ttcattgtta aaagtcggac agccagtaac agttcagatc
     2101 atcgacattg atgaatattc caaaaaaatc agtttatctt taagaacgtt agaaaaaaca
     2161 aatccagctg ttccatatcg cagaaaaagg tatttcacga ataaaaatcg taagatcgga
     2221 tttaccacta ttgcggagca gttacctatt tggatagaag aagcattatc tgaactagaa
     2281 cggaaagaaa ataagaaata aaaagtgtat tttttctgaa aatgtgctag aatttttatg
     2341 taagccatag aacaagttga tcgaggtgga aattttggca agcaaaagag ttgctggtac
     2401 aattatgttg catttggagg atggtactaa aaaatttttg gtccattcaa ttgatgacaa
     2461 gttggaattt gcgttagcgg aactttctga aggaaagact ggtttagcga atatacttaa
     2521 tttcttaaaa gaaatagttc atatcgatgt tagcaagatt agtttgatgg aattgacaaa
     2581 tacacatatt aaagaggaaa atgttccgtt gtttgtcttt gaaactgaac aaaagaatca
     2641 aagagaagaa ttaggcgaag gatatatttg ggaagaacca ggtcaaatgc gctctgttct
     2701 tggaacttac gaagtagagg gagtgccttt cttctaaaat acagattaag cgaacaaaga
     2761 agtttgaatt tttttcaaac ttcttttttt attgtttggt ccaaaaaata caatgaaatg
     2821 ttctgctaaa gatagttttt tagtgcattt tattttcaaa atatgttgaa aacggttgta
     2881 taacgaataa taataattga tcgaaaaatc atttcacaaa gtaatgctta atttatagtt
     2941 gaccaaggaa gaaatacatg ataaatttaa cagcgttgat tctgaagtgg atgttatatc
     3001 acatttctaa aaaatttaaa aaatagttat tgacatgtta ctaacgacat gataagataa
     3061 ctgagtcgtt aaaaaagaac cacttgcgaa gcttgattct atcgaaaaaa acttcaaaaa
     3121 agttcttgac aaataacaaa cgataagtta tgataaatga gttgctgaaa gatttcgaaa
     3181 aacgaaatcg aaaaaaactt ttaaaaaaga gttgacaatc gcttgtctct ctgatatgat
     3241 ataaaagttg ctacggcagc caaaaacgaa tcgggaaaca ctc
//
LOCUS       DAMYXM010000137         3257 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.142, whole genome shotgun sequence.
ACCESSION   DAMYXM010000137 DAMYXM010000000
VERSION     DAMYXM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.142"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..171)
                     /locus_tag="PNS21_002772"
     CDS             complement(<1..171)
                     /locus_tag="PNS21_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160399.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDA"
     gene            complement(312..647)
                     /locus_tag="PNS21_002773"
     CDS             complement(312..647)
                     /locus_tag="PNS21_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160400.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            complement(677..2269)
                     /locus_tag="PNS21_002774"
     CDS             complement(677..2269)
                     /locus_tag="PNS21_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160401.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTLEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEYGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTEREKIEYQRLNQAMEKYQAEPHKKTSGTLPGVFSISSDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEVGLKAAGRK
                     MMETLFEKEKLNDTGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNSLTKVVKEPVRYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDENRTKLSPYTREKLDELTQYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QTQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            complement(2289..2711)
                     /locus_tag="PNS21_002775"
     CDS             complement(2289..2711)
                     /locus_tag="PNS21_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160402.1"
                     /translation="MAKATRKHIYLYDMHIEVLDEIISTHDDISNYSEAVRHLCMMYD
                     PKTEEKISPSQIKLNAIGKEVSILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            complement(2750..3028)
                     /locus_tag="PNS21_002776"
     CDS             complement(2750..3028)
                     /locus_tag="PNS21_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160403.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHALLDYKKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIEQ"
BASE COUNT          844 a          656 c          467 g         1290 t
ORIGIN      
        1 cgcatcgacg aacgtcttcg tagtcagatt taacgttaaa atatcgccac tttgatatgc
       61 ttttgccgct ttgtctgtga ctacaaaagc tctgcctgtt tgaatttctg tcacaaaaac
      121 aagattgtct ctgctttttt caacaacaac tccaatcgtt gaataagcca tttcttcttc
      181 ctcctttctt ttgatataat gtagacggaa atcgcaccac tcaatgaatt ggcggaaaac
      241 aattcagtga aaaaaaagag tcctggacaa tcacgttcag gactctttta cttcttttat
      301 tcacttaaca actaacattc tgtgagtgaa atagactctt caatgctttt acacgggaag
      361 ttattgatcc cctcttcatt catttcccaa gtagtataat aaggcgctaa cttgccctca
      421 tattcttgaa tgataaaata tttttcaccc ttcacatgat tacaacagta cacaccagta
      481 gctgggtact caacgactaa aaattgattt ggatcaaact gttcgtttat caacatttct
      541 cttatctgaa acaaatgctc tttttctaaa caatcgggaa gttgttcgat tcgttcttct
      601 cttaatgatc cttctataac tttccattga tctaaatatt gtttcattgc tattcctcct
      661 actgtcttta ctgtctttac tgtctttctt gttccatttg gattcttcgt tgttgctctt
      721 cgtatgcttt acttgcgtgg tatttttctt catgggcatt aatcgttcga tttaattgac
      781 gcaaactccg ctgcgtgaca actctttgtt ggaattgttt cctgcgcttc atgaattctt
      841 cgtaactata tttgtattca ttcgttggca attgtttaag cgttcgaaag gatcgttcat
      901 tgtaaataga ttgcgtctga tcaacacttt ctattaatgg ttttatttga ctatcctgtc
      961 tcttcagctc tgttaggatg gcatttcctg tgcgcttttt taaatcaaag agagcatttt
     1021 tctctgcatc ctttgattct ctcttgcttt ccccataaag actttttgct tcttctttca
     1081 atgcttttga aatctgtata tattcttggt attcaggttc ttttgttaac acatattggg
     1141 ttaactcgtc gagtttctct cttgtgtatg gtgaaagttt agttcgattt tcatccccat
     1201 aattccattc cgaacgtttg tcaggtaagg ttttatatac ttcatttaat aagttcattg
     1261 cgtgtcgatc ataacgtact ggctctttta ccacttttgt taagctgtta cgcaaatcac
     1321 ttttccgcgt taataaattt cgtttctcca tgcctatcat tcgatccttt tcataagaaa
     1381 aacgttctaa ttcatgcaca taattggtct tcattgcgtc taatgttgct tgtcttctct
     1441 ttcctttcgg tacgatataa ctcacaccat tttcgacctc agtgtatggt ttacgcgtat
     1501 tttctttttc aacaactgca aaatgaatat gaatatgctt agtgttacgg tgaatggtag
     1561 ccatccaata tcccgtatca ttcagttttt ctttctcaaa caacgtctcc atcattttcc
     1621 ttccagcagc ttttagtcca acttcatcta aagtatctgt tttgggatcg tacaatccta
     1681 ttttttcaag atacgaatta tcatgagaaa caacatcttg ataaatgatt gagccttttt
     1741 gttgccctct ttggaagatc tgcttaatct ccttcagatc acttcctttt acttcgtctg
     1801 aagatataga aaaaactcca ggaagagttc ctgaagtttt tttgtgtggt tctgcttgat
     1861 atttttccat tgcttgattc aaacgttgat attctatttt ttccctttcc gttagttcct
     1921 ttttttgttc taacgcatag tgacgcgtca tataagaaag atatttggta aaatcttgtt
     1981 cctctaactt gtcaaaattg attccatatt ccatcaacga ttgcgcttca ttcgacgttt
     2041 cactgaattc tttatccgtc ttactctccg aaaaagtttc tccttcagga atataaaaat
     2101 cctctaaagc attcttgatt cgttgcagtt cctgttcttc ttcgagcgtg cgccgttcca
     2161 tttcttccag cgctttttct cttgtcatat aatcaatgta tgttgaaaaa gattttttat
     2221 tgggcaaggt aaattgacaa gtgagaatga ctgcaggtga tttactcatc acgttctcct
     2281 tctaattttt atcgttttaa aaaccttgtt ggatcaaata cttcactttt tgtagtcact
     2341 gattcgctta ttgttttctt tatcttagga gaatatttct tcgtttgatt tgcttttatc
     2401 ttgccttcca cacgctcttt cgcttcttga taaacagttg aatttgtacc aattaatatt
     2461 cctgtatctt ttaaatgaag atctgcaaac tccgaaacca tttcagttaa aatagaaact
     2521 tcttttccaa ttgcatttag tttgatttga ctgggactta tcttttcttc agttttggga
     2581 tcatacatca tacacaaatg tctaactgct tcactataat tggatatatc gtcatgagta
     2641 gaaattatct catcaagtac ttcgatatgc atatcataaa gataaatatg ttttcttgtt
     2701 gctttcgcca aagttgattt cctcctttta taaattcaca atattatttt tactgttcaa
     2761 ttaacaaggc gattaattga ttatttgttg caatatattc ttctaaaatt ttcgtattat
     2821 aagtttgaat atccacaaat ttttctaaca accgatcaaa ttgatctttt ttataatcca
     2881 gaagagcgtg atcaaaatca ttttcgatca aaacttttag gtatcgattt cggctccatt
     2941 tagtcccagt acgttcactt aattcaatac atcttcggtc aattctttga atcgtttttt
     3001 catcaatatc tcttattttt acttccataa atatctcctt ttgactatat gttataacgt
     3061 gtgtagcaat gtgtgtagct acacacattg ctacacatat ttaaatttta ctacccacaa
     3121 atgaactttt gtgctaccca cattcatatt tttgctaccc ataaacctac acataaattg
     3181 aaaattttgg ggtcatttat ttttttcaga aacgtgatag gcacagccta tctacccact
     3241 ttattttgaa agtgggt
//
LOCUS       DAMYXM010000138         3188 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.143, whole genome shotgun sequence.
ACCESSION   DAMYXM010000138 DAMYXM010000000
VERSION     DAMYXM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.143"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            185..481
                     /locus_tag="PNS21_002777"
     CDS             185..481
                     /locus_tag="PNS21_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160404.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            483..905
                     /locus_tag="PNS21_002778"
     CDS             483..905
                     /locus_tag="PNS21_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160405.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDEIISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            924..2516
                     /locus_tag="PNS21_002779"
     CDS             924..2516
                     /locus_tag="PNS21_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160406.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            2537..2872
                     /locus_tag="PNS21_002780"
     CDS             2537..2872
                     /locus_tag="PNS21_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160407.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            3018..>3188
                     /locus_tag="PNS21_002781"
     CDS             3018..>3188
                     /locus_tag="PNS21_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160408.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDA"
BASE COUNT         1260 a          471 c          637 g          820 t
ORIGIN      
        1 ccccactttt aaaaaaagtg gttggatagg ctgtgcctat cacgtttctg aaaaaaataa
       61 atgaccccaa aataccaata tataggatag tacaaggata gcaaaggata gtaaaaggat
      121 agaggtagga tagcgaaaag gataggatcc ttttcactat cctatattat attggaggat
      181 atttatgaat gttaaaatta gagatttaga tccaaaattc atttctgaaa ttgatcgaag
      241 atgcgttgaa ctaagtaatc gtacaggaaa taaatggagt cgtaatgatt atttaaagct
      301 actcgttgaa aatgattttg atcgtccttt gatggaatac aaacaagaaa agtttgacca
      361 gttattagaa aagttttcag aaattcaaag ttataacaca aagatattag aagaatatgt
      421 tagccaaaat aatcgtatta tagaaatctt gattgagcaa aatagaggaa gtgaactttg
      481 atatggcaaa ggttatagac aaacacattt actttcacca aacacatatc gatgtattgg
      541 atgaaattat ttctactcat gcagaggtta aaaactatag tgaagctgtt cgctttttat
      601 gtatgaacta tgatccagaa gtggagaaaa aagtaaattc caatcaagca aagttgaatg
      661 gaatgagtaa agaaatttct attttaaccg aaatggtttc agaatttgcg gatcttcatt
      721 tgaaagatac aggaatatta attggtaaaa attcaactgt ttatcaagaa gcaaaagaac
      781 gagtggaagg aaaaattaaa tccaatcaaa caaaaaaata ttcccctcag ttgaagaaac
      841 ctgtgaatga atctgttcat gccagagtag atccatttga tccaacgaag attctaaaaa
      901 gataaaaatg aaggaggata ataatgactc aatcgccttc agttattctc acttgtcaat
      961 ttactttgcc gaataaaaag tcattttcta cttatattga ctatattaca agggaacagg
     1021 ctcttgaaga aaaggaacga cgtacgcccg aagaagaaca ggaacttcaa cgaatcaaga
     1081 atgctttaga ggagttttat attcctgaag gagaaacttt ttcggagagt aagacgggta
     1141 aagaattcag tgaaacgtcg aatgaagctc aatcgttgat ggaacatgga atcaattttg
     1201 acaagttaga ggaacaagat tttaccaatt atctttctta tatgacacgt cactatgcgt
     1261 tagaacaaaa gaaagaactg acgcaaagtg aacaaaaaga ataccaacgc ttgaaccaag
     1321 caatgaaaaa atatcaaaca gaaccccata aaaaagcttc aggaaatctt cctggagttt
     1381 tttctatagc ttcagacgaa gtgaaaggaa gtgatctgaa ggagattaag caaatcttcc
     1441 aaagaggaca acaaaaaggc tcaatcattt atcaagatgt tgtttctcat gataattcgt
     1501 atcttgaaaa aataggattg tacgatccca aaacagatac tttagatgaa gaagggttaa
     1561 aagcagctgg aagaaaaatg atggagacat tattcgagaa agagcaactg aacgaaacag
     1621 gctactggat ggcaacgatt caccgtaaca ctaaacatat ccatattcat tttgcagttg
     1681 ttgaaaaaga aaatacgcgt aaaccttaca cggaggttga aaatggtgtg agttatatcg
     1741 taccgaaagg taagagaaga caagcaacat tagacgcaat gaagaccaat tatgtacatg
     1801 agttagaacg tttttcttat gaaaaggatc gaatgattgg tatggagaaa cgaaatttat
     1861 tgacgcggaa aagtgatttg cgtaacacct taacaaaagt ggtaaaagag ccagcgcgtt
     1921 acgatcgaca cgcgatgaac ttattaaatg aagtatataa aaccctacct gacaaacgtt
     1981 cggaatggaa ttacggggat gaaaaacgaa ccaaactttc accatacaca agagagaaac
     2041 tcgatgagtt aaccaaatat gttttaacaa aagaacctga atatcaagaa tatatacaga
     2101 tttcaaaagc attgaaagaa gaagcaaaaa gtctttatgg ggaaagcaag agagaatcaa
     2161 aggatgcaga gaaaaatgct ctctttgatt taaaaaagcg cacaggaaat gccatcctaa
     2221 cagagctgaa gagacaggat agtcaaataa aaccattaat agaaagtgtt gatcagatgc
     2281 aatctattta caatgaacga tcctttcgaa cgcttaaaca attgccaacg aatgaataca
     2341 aatatagtta cgaagaattc atgaagcgca ggaaacaatt ccaacaaaga gttgtcacgc
     2401 agcggagttt gcgtcaatta aatcgaacga ttaatgccca tgaagaaaaa taccacgcaa
     2461 gtaaagcata cgaagagcaa caacgaagaa ttcaaatgga acaagaaaga cagtaaagac
     2521 agtaggagga atagcaatga aacaatattt agatcaatgg aaagttatag aaggatcatt
     2581 aagagaagaa cgaatcgaac aacttcctga ttgtttagaa aaagagcatt tgttccagat
     2641 aagagaaatg ttgagaaacg aacagtttga tccaaatcaa tttttagtcg ttgagtaccc
     2701 agctactggt gtgtattgtt gtaatcatgt gaatggtgaa aaatatttta tcattcaaga
     2761 atatgagggc aagttagcac cttattatac tacttgggaa atgaatgaag agggaatcaa
     2821 taactttcca tgtaaaagca ttgaagaatc catttcactc acagagtgtt agttgttaag
     2881 tgaataaaag aagtaaaaga gtcctgaacg tgattgtcca ggactctttt tttcaatgaa
     2941 ttgttttccg ccaactcatt gagtggtgcg atttgcgact acattatatc aaaagaaaga
     3001 gaagggagga agcagaaatg gttcattcaa cgattggagt tgttgttgaa aaaagcagag
     3061 acaatctcgt ttttgtgaca gaaattcaaa caggcagatc ttttgtcgtc acagacaaag
     3121 cggcaaaagc ttatcaaagt agcgatattt taacgttaaa tctgactacg aagacgttcg
     3181 tcgatgcg
//
LOCUS       DAMYXM010000139         3140 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.144, whole genome shotgun sequence.
ACCESSION   DAMYXM010000139 DAMYXM010000000
VERSION     DAMYXM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.144"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            25..669
                     /locus_tag="PNS21_002782"
     CDS             25..669
                     /locus_tag="PNS21_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160409.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            680..1486
                     /locus_tag="PNS21_002783"
     CDS             680..1486
                     /locus_tag="PNS21_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160410.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            1509..1738
                     /locus_tag="PNS21_002784"
                     /pseudo
     CDS             1509..1738
                     /locus_tag="PNS21_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1759..2832
                     /locus_tag="PNS21_002785"
     CDS             1759..2832
                     /locus_tag="PNS21_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HDI5160411.1"
                     /translation="MKKTYQKFLWISYIVLFIVGFLVSVSVIVGTLFLLFSDEDLVLK
                     GLMSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIEYTWLPLKRNSKR
                     KEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQIAASNIFTAVMTL
                     MCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIK
                     NFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVD
                     FGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYNFYKVEASLRYGI
                     GKLRLDGSNIQQNKPYVLGKVVI"
     gene            2876..>3140
                     /locus_tag="PNS21_002786"
     CDS             2876..>3140
                     /locus_tag="PNS21_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160412.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESK"
BASE COUNT         1218 a          442 c          627 g          853 t
ORIGIN      
        1 aaaaaataga tcaggagaaa aaatatggaa aataaaacct taaaaatcgt cttaacagct
       61 gcaattagcg ttagtttaat cggcaatatt gcgttgtttt ggcagcttaa agaagaacaa
      121 gagacaaata cctataaaat tgaagaagtg gctaaaaacg cgtctgataa gctcaaaaaa
      181 aattcttctg aagcacaggc gaagattgac aagttaaatc aaaccatcaa tgagaaaaac
      241 caagaaatcg atcagctgaa gcaacaagat ggcacaaaag caaaagagga agtaactgaa
      301 gcacaaagaa agacggcaga agagttcggt caattggcca ttgataaatc attaagcaga
      361 aatgaattaa cggaaaaact aaaagatgtc gcaacccaag aagtgatcga caaattaagt
      421 cctgtagacg atgatcacca acatgatgat tacggaagtt actcttttac attaggtgac
      481 gtgcaaactt acgctcaaac aagcagtgta aaagaggaac aaaattttgt cgtatttgtt
      541 gattatacaa tcggcaatcc tcaattcaaa gatgtcaaac cacaaaaaat aaaaggtgga
      601 ttaactgtca ctgaaaaaca agtagatggt caatggaaag tcacagactt tagttatttc
      661 actcgttagg aggaagaaaa tgagtaagaa aaaaggagca agacaacgtt ttaaaaaacg
      721 aaaaagttat cttagatggc tggggatctt tatgattctc ggctttttga ttcttgctag
      781 tccgatttac acacataaaa aggtagacat accagatgaa gtaagcacga acgcattttt
      841 aaacacgaag gaactgacga tggtttcaaa agaaaaagga gcgaaccagc aattagtttt
      901 tgaatttgcg atcgacgatg aagatcagag cttattaaaa gaactagcta atcttaatta
      961 tcgtgtagaa gtaaagacag ctaaagggga ttatcaagca ataaaagcca aagtgatcaa
     1021 ggtgtctgat gattattttg tagtgaagtt agcacatgta ccagaagaat atattgctat
     1081 gcgattaact attattcctg aaaaaattga tccaaaggtg gatatgcaag aaccgcaaga
     1141 tctgattttt tatattcacg aagataaggt caaagatcac gtgaaagacg aagattatga
     1201 acaacatgcg atcaattaca aagtgaaagg atacaagaaa gaacaaaaag cagcacaaaa
     1261 acaaattgaa tccttccaag caaccattga tttgaatgaa gaattagtga agaaactaaa
     1321 agatcaattg ccttatcaag tcgcagaaga tcaggaaaat actgaaaata agattaatag
     1381 ttaccagcaa gaaattgaaa catcgaaaac gcaaatgaaa gatcaagaag agagcattca
     1441 aaaacttcaa gaaaaaattg atcgtatgac aaaagaaaat atttagaaga ataggtggag
     1501 taaaagaaat gaagcgagcg aacttggtcg gcaaagatac tttcgctgag cagaaagtat
     1561 tgattaaagc ctatctcaaa gttgtaaaag ctaaagaaat atgtcaagaa gtgaattatc
     1621 taacacaaca atatctgagt tgaagaatgg tagaaagagt ttagatacct taaaattcag
     1681 atcatttgaa gctctatata tagctgctaa aaaatatttt acagctgata ataaataaaa
     1741 aattaaggtg ggttctatgt gaaaaaaacg tatcaaaagt ttttatggat tagctatatc
     1801 gtcctattta tcgttgggtt tctcgtcagt gtgtccgtga tcgtcggaac gctttttctt
     1861 ttgttctctg acgaagatct ggtacttaag gggttaatga gtacgttatc gtgtgtcatg
     1921 ttactttatt tatgtataca gatcaacgaa attatgcccc ctcattggca agaaaagaag
     1981 aaagaattta aggaagaaat cgaaataaaa tccaatgact ggattgaata cacatggctt
     2041 cctctgaaaa gaaatagcaa acgaaaggaa ttttgggcat tgcaattatg gccaatagga
     2101 aaacttttta tttatatggc ttcacttatg gttactttaa tcggttattt ggtggtggtc
     2161 tatggggtgg cctattactt gttaagtgag ccaaaacaga tcgcggcgtc aaatattttc
     2221 acggcagtaa tgacattaat gtgtgtcatg tgcttttcta tttttagtta cttactcgtc
     2281 caatttatca ggcaaaattt aacaatgagt aaacttttca aaagtttatt ggacttactt
     2341 atcttaggca gtatgttaac gactctaaca gatcggttcg aacaatctga aaaaacggta
     2401 gaggggataa agaacttctt aacgacgatt gatcaattta taaatggata tatgtatttg
     2461 tttgaagtag gctttattct cactttgttt ggttgtgtat ttataaaact cattaaaaga
     2521 tcaatcgaaa aagaaatggt tattgatcta attccgacgc tgagcggcta tgagtattac
     2581 gttgattttg gaaagtatga ggagttgatc catacggata tcgttcacac gagtatccat
     2641 gaagtaaccg ttaagggcat cagatctact ttaaaagatg gacaggagac gaaaacaatc
     2701 gaccgaagaa aagaggtcta caatttttat aaagttgaag caagtctacg atatggcatt
     2761 gggaaattga ggttagatgg gagtaatatt caacaaaaca aaccatatgt tttaggaaaa
     2821 gtggtgattt agaacttcct atgaaagatg aataaataag gagaatcttt tgaaaatgat
     2881 tcttctttac agaattgtgc ttatagttgg cacggagtta aaaatcttaa agaaggagca
     2941 gttattcaaa gtgaaagaac atcataatca gttactatgt attaaaacgg gtacgatgaa
     3001 cattcaatac cgaacagaat atttagaatt aacgaatcaa actgattttt tgtcgattcg
     3061 agagcaacat aagcaagaaa aattaatggc tacttatagc caattgattg atgagtggga
     3121 acaattcaag gaatctaaaa
//
LOCUS       DAMYXM010000140         3105 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.145, whole genome shotgun sequence.
ACCESSION   DAMYXM010000140 DAMYXM010000000
VERSION     DAMYXM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.145"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(1..433)
                     /locus_tag="PNS21_002787"
                     /pseudo
     CDS             complement(1..433)
                     /locus_tag="PNS21_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(992..1309)
                     /locus_tag="PNS21_002788"
     CDS             complement(992..1309)
                     /locus_tag="PNS21_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDI5160413.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(1310..1564)
                     /locus_tag="PNS21_002789"
     CDS             complement(1310..1564)
                     /locus_tag="PNS21_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDI5160414.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(1824..2042)
                     /locus_tag="PNS21_002790"
                     /pseudo
     CDS             complement(1824..2042)
                     /locus_tag="PNS21_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2603..2689)
                     /locus_tag="PNS21_002791"
                     /pseudo
     CDS             complement(2603..2689)
                     /locus_tag="PNS21_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT         1044 a          542 c          428 g         1091 t
ORIGIN      
        1 tcatatttcc ttgattacct gaggcaatga aagacttatc tacttgaatt agggtgaaat
       61 tctcattcaa atatggtagt aaaattaata tatgtgaaaa tcgaatcttg agatagctat
      121 tccccttgag atattcactg atacttctac tcaaatcatt taaattttta taattatttg
      181 tacggaacgt atatatatca acatcagttt tctttacatg agaacttatt ctaaaatgaa
      241 aaagcctact ctcagaaaga attgaaaaat agatactttc agtagatact gaagattaaa
      301 caaaattaaa cttgtatcct tcaggcatag cacttttaac agtctgctgc attaactcgg
      361 ataatctttt gaaagtagta atatattttt taccagcaaa ccatacatat cttttttcat
      421 taagtatgtt cactatctct cctccattaa ttatatttag catcaaatag ttattgtacc
      481 tatattttat cccattcctt ttaaaacaag aatacgtaca aagtaaaatg ttgtaaatag
      541 tctagcaggg atgtatattt tcatgtacca acataatatt taagaagttt cccatagata
      601 tattggactt cctcttttaa aacacaaaaa taaatactac cttatttagg tagtatttat
      661 taaaagttac gtcaattttg attaaaaata tactacttga acctctcatg agtaaagtca
      721 cgaaattttt gggaatagag cgtacttgat atcttatttt ttcactaata gcagtgtctc
      781 ttattcacca agcgatcccc gtagttccta cggttcttaa tttttctcta gactaatgat
      841 tgtatcttta gaccttcggc cataacatgg agcaaaataa aaaggtacta agtcttatat
      901 atcaatcttc gagtaaccga cggttaacaa ccatatcgag cggcattaat cacccacctg
      961 tagaggatga gcgacttttg cctaattatt gttaaaagcc tagtttcatt tgtttagatt
     1021 ggaaaatacg tcctacaagt atctcttttt tttcatggtc gatccgataa aataaggcat
     1081 agcttttgcc gatcacgatc cgatatgttg ggatatccat tccatagatt tcggaaactt
     1141 cctcatgcat agttggaaaa tttttggtta gttctaaagc cttctggatc gcacctacaa
     1201 atctttttat gctggctgga gaaagagcaa gctcattttc ccattcttct attatcgttt
     1261 caaggctggt ctggaaactt ttagaataaa ctagcgtgta ctctttcatc tattcactcc
     1321 agccgtattt tccgaaagct tttaccattt caatttctga aactgttaaa cctttctcaa
     1381 agtcttggaa actttcttca atcaatttgc gatcgtgttc ttttgctatt tgagtagtat
     1441 ctaatatgaa taagttgcct tcttgtttgg cttggatagt gtctccgctt tttaatccaa
     1501 gagagcttgg aaggatcact cccacgctat tgccgatctt tctaaccttt agttcttttc
     1561 ccatgttatc acacttcttt tagtttttga taaataaatt ataacatgtt ataacgacaa
     1621 ttgccaacgg tgtttcttaa gggattccac tccatattat tattggacaa aataagccac
     1681 ttagaacgct gaaaacatat acaaacgata tcaaaatacg agtgaactac tcaccataca
     1741 cttccgttaa catccattta agtaggtatt tttattatat atagatttaa catatgtcgg
     1801 tcggttgatt gatatagcta aagttataac tcaaccgaag ccgattcatc tacacgaata
     1861 aagttgcgag tgttctcggc taattcataa aaattgttat gtgttccata tagcaatact
     1921 ggaataactt tattttcttg aatctcataa gcaactctat acacggtgac cgcatactta
     1981 aatccattag accaattacc tgataaacct ccagtttttt gctcttactt aaatggatca
     2041 atagcaattt catcacaaac ctatagagga tgagtgactt ctgcctaatt acgttaaaaa
     2101 atattgaaag taactctatg tagcaaaaaa tatctagaat ttcatttcct caaaactgat
     2161 cgatattgta tctaaaaata tatactacct aaaataggta gtatatatta aaatatgtgc
     2221 ttttgttaga aaaatcaata cataaattat catatctgta tatgtcattt aggtatttca
     2281 gttatgttat aatagttttg ctgtctgcta ccccggccta atctggcaag gggaggagga
     2341 gatataatga aatacctttt tgattttgtt tttgcaatca agccatttgc agaggtaact
     2401 gcacactaca tctgcaagtg gcttgattgc aaaattgcca aaaaagacag taagcctacc
     2461 agttaattct ataatataga aacgtaaaaa aaaaccccat cagcctacac gctgatgggg
     2521 tttgatgata taatgaaata cctctttgtt actcataatt ataccataat tagtcacgaa
     2581 tgcaaccaat agaaaaaact acttagaacg gtagatcatc gctaataaca atgcattgtc
     2641 caccaaatgg attttgtgaa gcattaaata cattgttatt aattgtcgct gcttttaact
     2701 aggcctctcc ccttctatta taaaagaaga tatatattat ttcttaatta cctactacct
     2761 taaaccaaaa tttaagtact acctaattta agtagtactt aaataatcta aaaaatagtt
     2821 atcccaattg ttatgctttt ataatacgat tatttgtaac aaaatgaata ttatataatt
     2881 aaacactacc atatttaggt agtgtttaat tattagcaaa acaaaaaata tttattctat
     2941 tctcttaact tcaactaagt tatgctgcac acctttatat aaatcatatg caactcatta
     3001 gctagacaaa ctacaagcat gtagttttat ctgttaatta tgtactacct aaataaagta
     3061 gtttataatt aacaggtata tatatttaaa aatttaagat taatt
//
LOCUS       DAMYXM010000141         3082 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.146, whole genome shotgun sequence.
ACCESSION   DAMYXM010000141 DAMYXM010000000
VERSION     DAMYXM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.146"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="PNS21_002792"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="PNS21_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HDI5160415.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          895 a          601 c          458 g         1128 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtcctt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa aagtacattt cttcatgttt ttttcggata tttgtgcttt ttaaccattc
     2521 agcagctgaa aacatataaa aagatatgta tgtattcttt ctgaaattaa gttcatttct
     2581 atgcgatgaa gcatgttttc aaaatattac cctttcttag attaaacttc agagtattca
     2641 aataacggaa taaggagcct tatcccgcat tactgaatga gactgtgaca taagtaaaaa
     2701 ggtgacttca aattccttct tcggaatttg aaatcacctt ctttataatt ataaagtatc
     2761 ggtttcacag catttctgaa tgattcctag aatcttaaac gaatgattgg agttatcaac
     2821 attttttcac tcgtttttag cgaaacaaaa aatgacattt gtcacagcct cattaaatta
     2881 ttgattcttt ttctttataa ttgaattgtc catatttaaa gtgtctagta ctctacatga
     2941 ataattaatt gctttcactc aaatacttat aatctattaa cttgtagttg tttaactttt
     3001 ctgaaaaagt tagacccacg taataatctt cccagctttc tctttcaatt aactgatcca
     3061 atttttgata atccataaat tc
//
LOCUS       DAMYXM010000142         3028 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.147, whole genome shotgun sequence.
ACCESSION   DAMYXM010000142 DAMYXM010000000
VERSION     DAMYXM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.147"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..198
                     /locus_tag="PNS21_002793"
     CDS             <1..198
                     /locus_tag="PNS21_002793"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160416.1"
                     /translation="IPPAIFFYYSILFDSRFKTLQQNQIVNNSLGTDFTYLDFIGDEG
                     KVANGFSEKIIQFQENRSKLN"
     gene            278..673
                     /locus_tag="PNS21_002794"
     CDS             278..673
                     /locus_tag="PNS21_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160417.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            998..1615
                     /locus_tag="PNS21_002795"
     CDS             998..1615
                     /locus_tag="PNS21_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI5160418.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1656..1955
                     /locus_tag="PNS21_002796"
     CDS             1656..1955
                     /locus_tag="PNS21_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HDI5160419.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1989..2156
                     /locus_tag="PNS21_002797"
     CDS             1989..2156
                     /locus_tag="PNS21_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI5160420.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(2201..2806)
                     /locus_tag="PNS21_002798"
     CDS             complement(2201..2806)
                     /locus_tag="PNS21_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI5160421.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTVMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         1062 a          444 c          543 g          979 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aaatagttaa taattcactt gggacagatt ttacttatct tgattttatt
      121 ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa ttattcaatt tcaagaaaat
      181 agaagtaaac ttaattaaga atcgtttact atttaaacag tgtccttttc ctttttaaat
      241 atatacgata aaataagtat atgaattgta aaaggacatg atgattatgg aagaaataga
      301 atggaataat ctgactcaaa ataataaaaa taattctgga tgtggaatta tctttatgtt
      361 tgttatagtt ttgggaatag tagtactttt aataactgtc aagatagtag attactttgc
      421 gaatactcct gaagaacaaa tacagagaaa acttcctgag tttgaaagag taccacctta
      481 tgatccaaga gataattcat ttcaagtaaa aaaattaaaa gagtctccaa atgaaatgtg
      541 gtttgaagtt acagttgaat caagcgactt caaagaaaaa aagaaagaat acaattatat
      601 tgtaaaggca aaagtatcaa aaagatttat ttttaaccct aaattagaaa tatatagtgt
      661 tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa agaagtcaaa ttctgatgtg
      721 aaaaataaaa tataccatgg aagacataaa agaaaaactg gataactagt ttatttaatg
      781 gcagaccgtt aaggcctgcc atttttatgt attttagcgg aataaatata atcatgcggg
      841 agtgtaaact tactgtttca aaaagtatac tttttgacat gccttgattt tctttgttta
      901 tgtatggcat aataaccaca taatacaatt tgaaacgttt ctgatttaat ctgattgttt
      961 tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg agaaaatttt tttatatccg
     1021 ggtcagtagt tatgatcaat cgatcgaacg acaactgatt tccgcgaagg aattaggaat
     1081 ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa gattttaaac gccctgaata
     1141 tcaattaatg aaacggatgt ttcgaaaagg agatttactc tatctacaat cattagatag
     1201 actaggcaga aataaacaaa tgattttaga agaatggcga gaattaacta aggaaaagca
     1261 gattgatatt tctgtgttag acatgcctct gttagatact gctagttaca agcaactaga
     1321 tgggttagag acgcttatct ctgacttaat tctgcaatta ctcagctata tggccgaaga
     1381 cgagcggaaa cgaattaata gccggcaaag agaaggcgta gaagctgcga aacaaaaggg
     1441 tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa tttcctgcga tctattctga
     1501 ctggaaagca ggaaagataa ctgcagtcta tgctatggat caattgggca tgaaacgaaa
     1561 taccttttat cgacgggtga aagaatatga acataccttg caccactttt cttgatagat
     1621 ataacagcta ttagacggga agggagagtt tgaaaatgga cttaacgaat caaagggtcg
     1681 tttttactgg aacgctgcag caaatgacac gaacccaagc catcggtttg gttcactctt
     1741 taaacggcca ttgtcaatca agtgttactt caaaaacaaa tttcctggtt tgtggtcagt
     1801 attctaagtc gttgtttgat gattctctct atacaaaaaa ggaacaaacc gcgcgggact
     1861 tgattgcgtt aggtcatgag attaccattt tatcagaagt cgaattttat gcgttaatta
     1921 gtcaacagtt gaaagaatgg caacggtatt tataatcgtt agtaaaattt acaaggagga
     1981 aaatttttat gaagcgaaaa tcttatgatc gttggggcat tgaaataact agttttaatc
     2041 gaaatattgc ttttcacgtg gagtgtgact gtggggaatt agcaaaaagt gtttatcaaa
     2101 aaaattactt ttactgtcct aaatgccatc gccgatacgg ggtacatgga acatagaaac
     2161 gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt ctacaaatca ttcgctttct
     2221 ttattttata aatagttggt cgagagatcc cgttttcttt tgcaatctct gcaactggaa
     2281 tgccttgctt taacatgtcc accacggttt taaaaactaa gcgtttttgc ggatctgttg
     2341 agttaataga atactcgatt ggtcgtccct tatatcggcc ggctttttga gccaataata
     2401 ctccttgccg ctgacgttca cgaattttct cacgctcgtt ttgtgcaata taacttagca
     2461 gtgataaaaa catatcgaac ataacggtat ccaggagttc atttccagta ttaaaatcta
     2521 aaaactgtgc atctaatatt tttaacgcta cttttttctg tttcataaaa gcaactgtat
     2581 tttttatatc ttcataatct cttcctaaac gatctagtga tgtaacgatg acgcaatcac
     2641 cttcacggat ataatttagt aatttttgaa actcaggacg ctcagtatct ttaccactgg
     2701 ctttttcttg gaataattta tcaatgttaa gtccttccat cattgccaat tgtcgatcca
     2761 aattttgatc ttgtgaagat actcgaatat agccaatttt tcccataaaa aactcctttt
     2821 taaaagtgat ataatttaca tgtaaaattt acttgtagtt ttactgctat catagcattt
     2881 ttaaaaataa attttatgta gacttaaaat ttacttcatg gagggtttaa atggatgaaa
     2941 tcaaaagtaa caaaaaagca tttggcattt atttaggaga acctagtaca gaacaattaa
     3001 accaattttt tggttctgtt gcgaagtt
//
LOCUS       DAMYXM010000143         2972 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.148, whole genome shotgun sequence.
ACCESSION   DAMYXM010000143 DAMYXM010000000
VERSION     DAMYXM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.148"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            130..495
                     /locus_tag="PNS21_002799"
     CDS             130..495
                     /locus_tag="PNS21_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI5160422.1"
                     /translation="MTAEVCTTTCIHKEKVASVKNNLKDKDLSTLLVLGKCFSDSSRI
                     KIFYALETYKEMCVCDLAAILNASIATTSHHLRFLKKNGMAKSRQYGKVVYYSLANEE
                     ILSAVQAFLKLSENSSMKA"
     gene            512..1123
                     /locus_tag="PNS21_002800"
     CDS             512..1123
                     /locus_tag="PNS21_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HDI5160423.1"
                     /translation="MLQNILSALAVYISTSIDYLFILLIIFSQNHTKKGLRQIFFGQY
                     LGTGILVAISLFAAYVLNFIPQDWIIGLLGLIPIYLGIRVAFVGEEEEEEGEVVEKLG
                     SRGTNRLFWTVSLITIASGGDNLGIYIPFFTSLSFSEIVTSLIVFAISVAVLCYISYK
                     FAKISFVSETLEKYERIIVPIVFIGLGIFIMIENGTIQTILGL"
     gene            1151..2812
                     /locus_tag="PNS21_002801"
     CDS             1151..2812
                     /locus_tag="PNS21_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDI5160424.1"
                     /translation="MSENKKYHEWILEGIDCANCADKVERGVAKVAGVANSSVNYMTE
                     TLSFEVTEEKEYLVLSNVQQKVKKLEPDITLKNKVDGRLIEINPALIKKSNLKDDKKN
                     YEWILEGIDCANCAAKVERGVATVPGVTNSSVNFMTETLSFEVAGENKTDVLASVKQT
                     VGTLEPDVVLRKKSDGTLIEHGTDSNKNSTSNKSISDKDETNQKQTNRAYLASLLGNK
                     TKITLIRLILGLGILATGIFLPMDGQASLGLFILAYLITGYDVVGRAIRNIFRGQVFD
                     ENFLMTIATLSAFYIQEYPEAVAVMLFYQVGEVFQDIAISKSRRSIADLMDIRPDYAN
                     LVTGNNVEKVAPEAIKIGNIILIRPGEKVPLDGKVVEGASAMDTSALTGESVPRSVKP
                     EDTILSGFINKNGLLKVVVEKPFSESTVKKILDLVQNASGRKAPTEQFITKFSRYYTP
                     IVVIAAALLAIVPPLLVPGATFNEWIYRASIFLVISCPCALVVSIPVGFFGGIGSASR
                     KGILVKESNFLEGLNDLKYVVMDKTGTLTKGFCCKVLNLLSNKVE"
     gene            2840..>2972
                     /locus_tag="PNS21_002802"
     CDS             2840..>2972
                     /locus_tag="PNS21_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDI5160425.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     "
BASE COUNT          955 a          456 c          618 g          943 t
ORIGIN      
        1 tatcaaaaac cttgttttat taatgttgaa tttatttgaa aggtctacag attaacatat
       61 tattgacaaa acaatattta aatagttata cttcattcaa acgtctgttt gaacgaggtg
      121 gtgaagttaa tgactgcaga agtatgtaca actacttgta tacataaaga aaaagtagct
      181 agtgttaaaa ataatttaaa agataaagac ctttcaactt tacttgtatt aggaaaatgt
      241 tttagcgatt cttctaggat taaaattttc tatgctttag aaacgtataa ggagatgtgc
      301 gtttgtgatt tagcagcgat acttaatgct agtattgcta caacgtcaca ccatttacgt
      361 tttttgaaaa agaacggaat ggcgaaatca cgtcaatatg gaaaggtagt ttattactct
      421 ttagcgaatg aagagattct ttccgctgtg caggcttttc taaagttgtc agaaaatagt
      481 tctatgaaag cttagtttgg aggtagttat tttgttacaa aacattttat cagcactcgc
      541 tgtatatatt tctaccagta ttgattattt atttatcttg ctgattattt tttctcaaaa
      601 ccatactaaa aaaggtctcc gtcagatttt ttttggtcaa tatcttggaa caggaatctt
      661 ggtagccata agtttgtttg cagcatatgt gctaaatttt attcctcaag attggatcat
      721 tggtcttctg ggactaattc cgatttattt agggattcgt gttgcttttg tgggcgaaga
      781 ggaggaagaa gaaggggaag tcgttgagaa gctaggatct agaggaacga atcgtttgtt
      841 ttggacagtt tccttaataa cgattgcttc tggtggcgat aatttaggga tttatattcc
      901 tttctttacc tcgttatctt tttcagaaat tgtcacttct ttaatcgtct ttgctatctc
      961 tgttgcggtt ctttgctata ttagttataa atttgcgaaa atttcttttg tctctgaaac
     1021 actagaaaaa tatgaacgaa tcattgtacc tatcgtattt attggattag ggatttttat
     1081 tatgatagaa aatggaacca tccaaactat attaggtttg tagagatctt attagtaaag
     1141 gaagcaaacg atgagtgaaa ataaaaagta ccatgaatgg atattagaag gaatcgactg
     1201 tgcaaattgt gcagacaaag tagaaagagg cgtcgccaaa gttgcaggtg ttgccaatag
     1261 tagtgtgaac tatatgacag aaaccttgag tttcgaagtg acagaggaaa aagaatatct
     1321 tgttttatcc aatgtgcaac aaaaagtaaa aaaattggag cctgatatca ctttaaaaaa
     1381 taaagtggac ggtaggctga ttgaaataaa tccggcatta atcaagaaaa gtaatttaaa
     1441 agacgataaa aaaaactacg aatggatatt agaaggaatc gattgtgcaa actgtgctgc
     1501 caaagtggaa agaggagttg ctacagttcc cggcgttaca aacagcagcg tcaattttat
     1561 gacagagaca ctgagcttcg aggtagcagg tgaaaataaa actgacgtgt tggctagtgt
     1621 aaaacaaaca gtaggaactt tagaacctga cgttgttctg cgaaaaaaat cggacggtac
     1681 attgattgaa catggaactg actctaataa aaattcgact tctaacaaat cgatttctga
     1741 taaagatgag acaaaccaaa agcagacgaa tagagcttat ctggccagcc ttctcggaaa
     1801 taaaactaaa attaccctta ttcgtttaat actaggatta ggaatattag cgacaggaat
     1861 atttctccct atggatggac aagcctctct tgggttgttc attctggctt atttaatcac
     1921 cggttatgac gttgtaggac gtgccattag aaatattttc cgaggtcaag tattcgacga
     1981 gaactttcta atgaccattg cgacgttaag tgccttctat attcaagagt atcccgaagc
     2041 tgtagcagtc atgctatttt atcaagtggg agaagtattc caagatattg ctatttcaaa
     2101 atctcggcgc tccattgcgg acctaatgga tattcgacct gattatgcca atttagtcac
     2161 aggaaacaac gtagaaaaag ttgctcctga agcaattaag attggaaata ttattctcat
     2221 tcgtccgggc gaaaaagtgc cattggatgg aaaagtagtg gaaggtgctt ctgcgatgga
     2281 cacttctgct ttaacaggcg aatccgttcc aagaagcgtg aagccggaag acactatttt
     2341 gagcggattt attaataaaa acggattgct aaaagtagtt gtagaaaagc ccttttcgga
     2401 gtctactgtg aagaaaattt tagatttggt ccaaaatgct agtggccgga aagcacctac
     2461 tgaacaattc attaccaaat tttctcgcta ctatactcca attgtggtta tagctgctgc
     2521 cttactggca attgttccgc cattgctagt accaggcgct acttttaatg aatggatata
     2581 tagggccagt atctttttag ttatttcatg cccttgtgct ttagtcgttt cgattccagt
     2641 tggtttcttt ggtgggattg gttctgcttc ccgcaagggg attttagtaa aagaaagcaa
     2701 tttcctcgag ggtttgaacg atcttaaata cgtggtaatg gacaaaacgg gtacattgac
     2761 aaaagggttc tgttgcaaag ttttaaatct actatcaaat aaggtagaat aatagaaaaa
     2821 gatagtagga ggaatgacga tgaatcattt taaaggaaag caatttcagc aggatgtgat
     2881 tattgtagcc gtgggatact atcttcgtta taaccttagc tatcgtgaag ttcaagaaat
     2941 cttatatgat cgtggcatta acgtttctca ta
//
LOCUS       DAMYXM010000144         2945 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.149, whole genome shotgun sequence.
ACCESSION   DAMYXM010000144 DAMYXM010000000
VERSION     DAMYXM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.149"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(53..988)
                     /gene="murB"
                     /locus_tag="PNS21_002803"
     CDS             complement(53..988)
                     /gene="murB"
                     /locus_tag="PNS21_002803"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDI5160426.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1061..1981)
                     /locus_tag="PNS21_002804"
     CDS             complement(1061..1981)
                     /locus_tag="PNS21_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDI5160427.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2094..2846
                     /gene="xth"
                     /locus_tag="PNS21_002805"
     CDS             2094..2846
                     /gene="xth"
                     /locus_tag="PNS21_002805"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDI5160428.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          823 a          601 c          509 g         1012 t
ORIGIN      
        1 agcaggacgg ccaccgtcct gctcttttac ttattttaag ttccattact tattaattgg
       61 ctaaacttct gcttgtttct gatattgctt gtttttctcc aatgatccgc acttctgttt
      121 ctaaagacac atcaaatttt tctttgatta cttcttggat gtgcgcaatc aattcaatat
      181 aatctgtcgc tgtcgcatga tcgatattga cgatgaatcc agcatgtttt tcagagactt
      241 gtgcaccgcc ccatttcagg ccttgcaatc ctgcttgttg gatcagctga cctgtatagt
      301 gtcctactgg gcgcttaaag acacttccgc atgaagggta ttctaatggc tgttttgatt
      361 gacgcaattc ggtcaactca tccattcttg ctttgattgc ttcataatcc cctgtttgca
      421 aactgaatcg agcttccaat acgatagcac gtaattcttg gactttactg tgacgataag
      481 aaaataccat ttcgtctttt gtcaaagttt tcaatgttcc atcttcaagc aaaagatcca
      541 cttcagcaaa aacatctttg atctctccgc catacgcacc tgcattcata taaaccgctc
      601 caccgacact tcccgggatg ccgcaagcaa attcaaatcc agtcaatgat tctgccaaag
      661 ctgcataagt cgtatcaatc agtgaggcac ctgcttccgc aatgaccatc gttcctttga
      721 catgaatctg tttcatttca gtcagcatga tgactacacc gcgaatcccg ccatcttgaa
      781 cgatcagatt gcttgcattt cctaatacca tccaaggtat atcatggatt cggcaataat
      841 cgatgatttg ctttacttct tcttgttttt tcgggaatgc aaggacatca gctggtccac
      901 ctgttttcgt aaacgtatag ttcatcaatg gttcatcaaa caatatagta atcgtggcta
      961 agctttcaac tatttcttct ttattcactg aggattttcc tttcttttct caaacgtcct
     1021 cattattcta tcacgcttaa tacaatcttg ccagtcttgt tcaaagttga tgacggtagt
     1081 gatctttaag cacttcctca cataattcat gacttcttac aacttctttt tgtttaagca
     1141 attctgtttc cgatttacct aaacggatct gagaaaagaa ggcctctacc attggctcaa
     1201 acccgcgttt gactagcgtg tttgtccagt cgccaaattc tttcactttt tttccatcag
     1261 catcttgaat gacaagctgg gtcagatcgt ttacttcata agtaccattt ttacttgtta
     1321 cttgatattt ttctatatta gcgccacttg ctaaatccat cgttaaaata gccgtttgat
     1381 tttgtgtttc taattgtaaa atggcatatt ccaaaaattc ctttgtttct cttatctggt
     1441 attttgttct caaaatgggt tcatctaata aatacacagc tgtatcaata agatgtaaaa
     1501 acaaatcata aaggacgaac tctgtcgttt cttttgccgc tattcgattt ttttgcaggt
     1561 ggatcaaccg cttatctggg atacgtttca attcttctac cataggagca aagcgccggt
     1621 tgaaaccgat catcaaaatt atttggttct tatcagctaa ttcttgcaat tcacgaactt
     1681 catttagatg gacactcagt ggtttgtcaa tataaacatg gataccattt tctagacatt
     1741 ttttggctaa ttcataatgt acttttgttg ctgcgtggat catacatgct tcgatttttt
     1801 cttctaaaag ctcatcgatc gtttcatata gatgttggaa cccgtactct ttttgaattc
     1861 tttcttttgt ttctttattt cttgttgcaa aataaaaatc aagtgttcct tgttttttcg
     1921 cataagttgg taaatatgct ttttctgcga tattccctac accgattaca ccaatcttca
     1981 tctactttcc tcctggatca tttagttact ttcatcttac accatttttt actaaaatga
     2041 ggtacaatag tatcgcaaag gtaaggaaat taattcgaag gagtgtgctt ttattgaaat
     2101 ttatctcttg gaacgtaaac gggcttcgtg ccatcgtaaa taaaaatttt ttagaagttt
     2161 ttcatgagtt agacgccgat tttttctgtc tgcaggaaac gaaactccaa gcaggacaaa
     2221 ttgatttaga tcttccaggt tattatcaat attggaatta cgcggaacga aaaggctatt
     2281 ccggcacagc gatttttgct aaaaaaccag cattgaatgc cacttacgga atgggtatcg
     2341 atatccatga tacagaaggc cgtctgatta ctttggaata ttccgatttc tttcttgtaa
     2401 cctgttacac gccaaattct caaagcgaat taaaacgttt agattaccga ttagaatggg
     2461 aagaagcctt ttataattat ttggaaaatc taaaaaaaca aaaaccagtc atcgtgtgtg
     2521 gggatctaaa tgtcgctcat caaaagattg atttgaaaaa ctggaaaacg aatcaaaaaa
     2581 atgctggatt cactccagaa gaaagagcgg ctctttctcg actgttggat aacgggttta
     2641 tcgatacatt ccgttatttt tatccaaccc aggaaggtgt ctactcttgg tggaactacc
     2701 gattcaattc aaggaaaaac aatgctggtt ggcggatcga ttactttttg acgagcaaag
     2761 atttagagcc aggactagct gatgcaaaga tccatacgga cattatgggc agtgatcatt
     2821 gtccagtaga attagatttg aaataaagag gttgtgaaaa agtcgtttag tttcgagtat
     2881 taagaaaaat tttgtgaaaa tggctttcca cattttgcat aaatttggca taataccgag
     2941 aaact
//
LOCUS       DAMYXM010000145         2857 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.150, whole genome shotgun sequence.
ACCESSION   DAMYXM010000145 DAMYXM010000000
VERSION     DAMYXM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.150"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            562..843
                     /locus_tag="PNS21_002806"
     CDS             562..843
                     /locus_tag="PNS21_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160429.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLEKFIAIQTKNTQVLNDYVHTNNQLIETLIGGK"
     gene            845..1261
                     /locus_tag="PNS21_002807"
     CDS             845..1261
                     /locus_tag="PNS21_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160430.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKT
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1422..1688)
                     /locus_tag="PNS21_002808"
     CDS             complement(1422..1688)
                     /locus_tag="PNS21_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160431.1"
                     /translation="MAFRFRCDRGGRKMTEKFPYEYYQNLEKLESAIQLVKLDLLDEN
                     LLKYKNITPERKGKLKKMQQLFDEEDGISDVIFDTWSEFHGEYN"
     gene            complement(1646..2620)
                     /locus_tag="PNS21_002809"
     CDS             complement(1646..2620)
                     /locus_tag="PNS21_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDI5160432.1"
                     /translation="MGKKRTIVNEWEKLTAPSNRRLNVGESVKMIKMDWSIDRITIVG
                     TLNENIYYHTQNDVLILDFEQLMRVNEGNGHLKAVGNNGWQLVDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKKFIHDLFLEPHFSRADVACDLIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQEAKRKIVPKEVETWWRLELQ
                     LRRGKATDWYEMVQESLDSFASPHYFPEDVKTLDRVMIKGLLYDHSEWSTISRDLKYR
                     LRKLLKQESQNDELTNHLRETFEESADELKRELDTWLLGLDVTEEEEK"
BASE COUNT          926 a          624 c          397 g          910 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatcattt cacgcttccg ctcttcattt cattcagttc tcggctttca caatctaaca
      121 cgccgtttcg ctaccagtcg ccttacaggc tccattctcg cttctcttac gtgttttacc
      181 gattgtgccg acaactaaac gaaatgaacg ctaaaacgtc ttagaacgca aaatacacgc
      241 ttataaaatc atttcatctt gattatatgc tcatcataat cgttcaattt gacatcattt
      301 ccgtaaactt tttttatttt tataagcaga aaaaagtcgc acatcaacat ttttttaaag
      361 agcataagga aacgtgtacc cactttctaa aaaaagcggg cagataggct gtgcctatca
      421 cgtttctaag aaaaaaattt gatatcactt tttcgccatt gtgactagct tatgtatagt
      481 gtttcagcaa aaaacagcta tacaaatact atattcgtat gatatacata aattatacac
      541 aaatgcgaaa ggagtttttc tatggacatt ttagttcgga agattgattc aaaggttatt
      601 gcaaaaatag acaaacgttg ccaagaactc acaaataaaa ctggcaaaaa atggagtcga
      661 aataaatatc taaaagtttt aatcgaaagt gattttgacc gtcaacttac gacttacaaa
      721 aaagaccaat tcgatttatt actcgaaaaa tttatcgcta ttcagacaaa aaacactcaa
      781 gtactaaacg actatgttca tacaaataat caacttattg aaacacttat tggaggaaaa
      841 tagcatgaga aaaacttctg ttcaaaaaca cgttaccctt tatcccagtc acgtggacgt
      901 tttagattcc attatcgaaa gaaatccaga tatcaacaat tactcggaag ccattcgttt
      961 tttatgtatg agttatgaac ctgataccaa cacgactact accagcaatc aagccaaact
     1021 aaatgctatg ggaaaagaaa tttccatcat gtccgaaatc attgcagaat ttgctgattt
     1081 atatctaaaa gatacaggcg ttcttattgg cacaaattct aaagtgtatc aagaagtgaa
     1141 agaaaaaacc gaagctaaac taaatccgtc gaatcgaaat cgaccaaaga atcatcaaaa
     1201 agaaacgagt caaacagtca cttctagaaa tttcgaccca acaaattttc taaaacgcta
     1261 aaaaataaaa cgagaatttt tttatttctc gttttatttt ttaggaaagc cggtcttaca
     1321 gcaagatttg caagttaccg gtaacttgcg gaaaagaaga agccgctcac gcgacttctt
     1381 ctttttgcat tttctgaaag ctaccacaat tcctaacttc gctaattata ttctccatga
     1441 aattcgctcc atgtatcaaa aatcacatct gaaattccat cttcttcatc aaataattgc
     1501 tgcatttttt tcaatttacc ctttctctct ggcgtaatat ttttgtattt tagtaaattc
     1561 tcatccaaca aatccagctt gaccaactga atagcactct ccaatttttc tagattttga
     1621 taatactcgt atggaaattt ctcagtcatt tttcttcctc ctctgtcaca tctaaaccta
     1681 aaagccatgt atccaattct ctttttaatt cgtctgcaga ctcctcaaat gtttctcgta
     1741 aatgattcgt caattcatca ttttgacttt cttgcttcag caatttcctt agccgatatt
     1801 tcaagtcacg actaatagtt gaccactcag aatggtcata caacaacccc tttatcatca
     1861 ctctatccaa cgtttttaca tcttcaggaa aataatgcgg actagcaaaa ctatccaaac
     1921 tttcttgaac catctcatac caatcagtcg cttttccacg gcgcaattgt aactccaaac
     1981 gccaccacgt ctcgacttct ttaggtacaa tttttcgctt tgcttcctgt tccaacttct
     2041 tgttgtacat ccgaatttgg cgttcgctgg cacgactacc ccaataagcc gtttctagct
     2101 taccagagcg tccataaatt ggtttgaagg acacagggtc aactacacga tattgtgtaa
     2161 taaactcatc aggcacatca ataagatcgc aggcaacatc cgctcgtgaa aaatggggct
     2221 ctaaaaataa atcatgaata aattttttca tagagcttgc taaaaattga ctaattttat
     2281 tggggttaaa gtcaatccgc cctttaccct cttgccattt caaaatttcg atataggcaa
     2341 tattttcttc gtgttgatct accaattgcc aaccattatt cccaacagct tttaaatgac
     2401 cattaccttc gttcactcgc attaattgct caaaatctaa aatcaacaca tcattttgag
     2461 tgtgataata aatattttca ttcaaagttc cgacaatcgt aattcggtca atcgaccaat
     2521 ccatttttat catttttacg ctctcaccga catttaaccg cctgttagag ggggctgtta
     2581 atttttccca ctcattcaca attgttcttt ttttacccaa attttgttcc tcctcaaaga
     2641 aaaaaagaga tttttggaaa gaatttccag agttcacgta actctaaaaa tccacatcca
     2701 aaaatctcta gcttgaagaa acctaaaaat attcgtataa tgaacttgag ttacggaact
     2761 aggctagtag tttccgtaaa ggtcgagagt gttagcgcac tgctcgacta tttttttgct
     2821 cttttttctc gttttctttg attatagcac tcctctt
//
LOCUS       DAMYXM010000146         2822 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.151, whole genome shotgun sequence.
ACCESSION   DAMYXM010000146 DAMYXM010000000
VERSION     DAMYXM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.151"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            95..1054
                     /locus_tag="PNS21_002810"
     CDS             95..1054
                     /locus_tag="PNS21_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1252 family transposase"
                     /protein_id="HDI5160433.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKIVKIAEYLKRSRTPIYNVINF
                     LKEGHTALEYYQQYKENKKRCGRRNIVLPKKQQSYIKEKIAQGWTPDVIIGRKEKPIS
                     CSVRTLYRQFKEKVFDESTLPMKGKRKPNGHQERRGKQAFKRNIVERETDYPTFKEEF
                     GHIEGDTIVGIHHKSAVITLVERLSKIIITLKPEGRKAIDIENTLNHWFGSIPRHLFK
                     SITFDCGKEFSNWKALSNQHDIAIYFADPGTPSQRALNENSNGLLRKDGLPKNMDFNQ
                     VDQAFISSVANKRNNIPRKSLDYQTPLEVFMSYMNEDILSSLI"
     gene            complement(1487..2059)
                     /locus_tag="PNS21_002811"
     CDS             complement(1487..2059)
                     /locus_tag="PNS21_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI5160434.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2075..2680)
                     /locus_tag="PNS21_002812"
     CDS             complement(2075..2680)
                     /locus_tag="PNS21_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HDI5160435.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          888 a          579 c          469 g          886 t
ORIGIN      
        1 gccgattgta aaattaagct agacaaataa ataagccatt cgtgctacac tcaaaatgta
       61 attccgacta aagaaaacat aggagtgatc acgaatgacc tacacccatc ttaccacgga
      121 tgaactggtg atgatagagt cttactacca ccaaaacatc aaaattgtga aaattgctga
      181 gtacctgaaa cgatctcgaa caccgattta taacgtcatt aatttcttga aagaaggcca
      241 tacggccctc gaatactacc aacagtataa agaaaataag aaacggtgtg gcagacgaaa
      301 catcgtcttg ccaaagaaac aacagtccta cattaaagaa aaaattgccc aaggctggac
      361 gccagacgtt atcattgggc gtaaagagaa accaatcagc tgttccgtaa gaacccttta
      421 tcgccagttc aaggaaaagg tgttcgatga atcgactttg cccatgaagg gtaagcggaa
      481 accaaatgga catcaagaac gccgaggaaa acaggctttt aaacggaata tcgttgaaag
      541 agaaactgat tacccgacat ttaaagaaga gttcggccat atcgaaggcg ataccattgt
      601 aggcattcat cataagagtg ctgtcataac tttggtagaa cgtctatcga aaatcatcat
      661 caccttgaag cccgaggggc gcaaggcgat cgatattgag aatactctga accactggtt
      721 tggatcgatt cccagacatt tatttaaatc cattaccttt gattgtggga aagaattctc
      781 taattggaag gctttaagta accaacatga catcgctatt tatttcgctg atccagggac
      841 tccttcgcaa cgagcactga atgaaaattc caatggcttg ctgcgaaaag atggtctgcc
      901 aaaaaacatg gattttaatc aagtcgacca agcttttatc tcttcagtgg cgaataagag
      961 aaacaatatt ccacgaaagt ccttggatta tcaaacaccc ctggaagtat ttatgagtta
     1021 catgaatgaa gatattttgt ctagcttaat ttgacaaatc aaattattta aaactttgca
     1081 acagaacctt agcagcagtg ctgattttta tgatagttat ttggttctta aatgaaagat
     1141 ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca atagattatt
     1201 tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg aacgatatgt
     1261 gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc taatatagta
     1321 agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca cattttttga
     1381 gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag actcatatct
     1441 agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa ctttcttcaa
     1501 ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct tcaacttcct
     1561 tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt gtgattgttg
     1621 ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt tttccttctt
     1681 gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac gtccgtgtca
     1741 ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg atcgttacgt
     1801 tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga gcaaatcgat
     1861 caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc tgattgaatt
     1921 ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc ttctctacgc
     1981 cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt gagacacgtg
     2041 catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc gtatactcag
     2101 aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg cgatcgtcaa
     2161 ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt atgggacttc
     2221 gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca actttttgca
     2281 attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt ccttctcgaa
     2341 atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa gaagtttgtg
     2401 gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca aatcgaaaat
     2461 ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata tccgaaaata
     2521 gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca tttatttttc
     2581 cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc ttagccaact
     2641 caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc cttaacttta
     2701 tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt tatgtgtcaa
     2761 aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt gcacagtcta
     2821 gt
//
LOCUS       DAMYXM010000147         2718 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.152, whole genome shotgun sequence.
ACCESSION   DAMYXM010000147 DAMYXM010000000
VERSION     DAMYXM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.152"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(130..1278)
                     /locus_tag="PNS21_002813"
     CDS             complement(130..1278)
                     /locus_tag="PNS21_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI5160436.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1295..1699)
                     /gene="tnpA"
                     /locus_tag="PNS21_002814"
     CDS             complement(1295..1699)
                     /gene="tnpA"
                     /locus_tag="PNS21_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HDI5160437.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            1861..2718
                     /locus_tag="PNS21_002815"
                     /pseudo
     CDS             1861..2718
                     /locus_tag="PNS21_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          906 a          423 c          433 g          956 t
ORIGIN      
        1 ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat agcgtatttc
       61 tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta cggttcttga
      121 tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca gaatattgat
      181 actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc attctcgaat
      241 ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac tagacggaaa
      301 ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt gtaatttagt
      361 cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat ttcgcaacat
      421 accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca tttctgtact
      481 caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc tagctacttt
      541 gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat ttttagccaa
      601 taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca ttttggacgt
      661 gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca taatacctaa
      721 gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt tacacaacaa
      781 agaaatatag tatttaccgc tagaatgacg tgatattgta gcggatttga taatcccttt
      841 tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt tgatgaattt
      901 actatcaatc aaagcaactg taccattttg attattcgtt gtataactct gaacaggatt
      961 tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat tcttgtatgc
     1021 tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt tcaaaaacgg
     1081 aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag aatcattttt
     1141 aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct tacggacaca
     1201 accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc tgaatttgta
     1261 tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt ccttgatttt
     1321 ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga caaaaacttt
     1381 tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca cgttttatca
     1441 atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt ttaggttgag
     1501 ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa gtaatgtgat
     1561 acgattctga aattctttca aaagtagttt tggcataatc agatatttca tcatcaatca
     1621 cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat actgaatgat
     1681 tatttgtatc taattccatg ttatatcaac tcatttcttg tatagactga ttgtaacatg
     1741 gaacaaaata aaaaggcaat acgccttagt tgtcggtctt cgagttacca acggtaaccc
     1801 tcataccgaa cggcattcat cacccaccta tagaggatgg gcgacttctg cctaattctg
     1861 ttaaaaatta gctttgagcc aactgattca tttcagtatc ttagcaaaga tattgaaatt
     1921 gaagatattt ataaaatttt agctcaagag ctatctaaaa atgttattag tttgccaaag
     1981 gttttttatt ttccagccga aagaactggt ataaatgtct ttaagaacga actaaatgaa
     2041 agtcgcttaa aaacatatga taccttgctg aatactgtgc aatttactaa tttaactaac
     2101 caaaaaaaga aagctgccct caagtacgat ctgttaaaaa agaatattga gctgttaatg
     2161 gagacaacgg ataatagttc ttatccaaag ccaatatcgg attatattaa ttatctgaat
     2221 aacatgaaaa atagatatga acagaaaaat gtcaataata tagcatcata tattagagat
     2281 gagattatta tgggtaaata tgaaattgac gaaaaagata attctgtaag ttttagacaa
     2341 aagtttggta aaaatagata tagaaaagaa cgaattcctt ttcacgttgt gtcttcttcg
     2401 attaaatctc tatatggatt agattattac ttagaaaatt caagtaaaga aggagactat
     2461 cttattattg atgagccaga attgagttta catccagaaa atcaggttaa gttagcaaaa
     2521 gtaattacag atatagttga gagtaaaatt aagctaatta cttctaccca tagtgattta
     2581 tttgtacgtg agcttacaaa tattgttatt gaaaataaat taaatagtaa gaataataaa
     2641 aaaaataaat tgtctgataa aaatataggc atatataatt ttgtaggaaa taatgatata
     2701 aatgaattag aaaaaatt
//
LOCUS       DAMYXM010000148         2652 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.153, whole genome shotgun sequence.
ACCESSION   DAMYXM010000148 DAMYXM010000000
VERSION     DAMYXM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.153"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            87..1094
                     /locus_tag="PNS21_002816"
     CDS             87..1094
                     /locus_tag="PNS21_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDI5160438.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1106..2110
                     /gene="neuB"
                     /locus_tag="PNS21_002817"
     CDS             1106..2110
                     /gene="neuB"
                     /locus_tag="PNS21_002817"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HDI5160439.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2107..>2652
                     /gene="neuC"
                     /locus_tag="PNS21_002818"
     CDS             2107..>2652
                     /gene="neuC"
                     /locus_tag="PNS21_002818"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HDI5160440.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNN"
BASE COUNT          972 a          336 c          525 g          819 t
ORIGIN      
        1 cattattgat aattagttta gttatatatt ttttaaagat taagaatcga attatagatg
       61 actataattc ataggaggag atataagtgt ttaaaggaaa aacaatatta gtaactggcg
      121 gaactggttc cattggaagt gaaattgttc gtcagctttt aaaatatgaa ccaaaagcta
      181 tcagaattta tagtagaggt gaagaaaaac aattttatat gcaacaagag ctctcaaatc
      241 acactaattt gagattttta attggtgata tcagagactc tcaaaggatt gattatgcat
      301 gtcgtgatgt ggatattatt tttcatgctg ccgcaatgaa acacgttcca gcaagtgaat
      361 ataatcctat ggaagctgta aaaacaaatg tgatgggaac tcaaaatgta atagatgcag
      421 cgatcaaaaa taatgtaaaa cattttgtag gtatcagtac agataaagta gtgagtccta
      481 ctaacaccat gggtgcgaca aagttattga gtgaaaaatt aataatagct gccaatcagt
      541 ataaaggaaa aacagaaaca atattttctt gtgtaaggtt tggaaatgtc atggggtcaa
      601 gtggatctgt tattcctctt tttagtaaac aaataaaaaa caataaagat attactttga
      661 cagatgttga tatgacacga tttatgatgt caataccgga tgctgcaaga ttagttctgt
      721 ctgctgctat gaattcaaaa tttggagaaa catatgtctt aaaaatgccg gcaatcaata
      781 ttttaacttt agctcaatca ttaataaaaa tatataatga tacacatgat gaaaaatata
      841 gaggtgaaat aaaagttgta ggtatcaggc caggagaaaa attatatgaa gagctaatga
      901 ctacagacga aattgaacgc tcatatgaaa atgataaaat gtatatcatt ccaccacact
      961 atcatgctac tgacgaactg tacaaagatt atgaaaaaat taaaatcact gcatactctt
     1021 cagatgcaac agaattaatg agtggacagg aatttattag aatcgcaaaa gagaataatg
     1081 tcgtgacttg gtaaggagtt ttatgatgaa atcagaagta tatgtaattg cagaaattgg
     1141 tgtaaaccat aatggaagtc tagatttagc tttagaaagt attgataaag ctaaagaatg
     1201 tggagctaac gcggttaaat ttcaaacatt taaaacgaat aagctagttt ccaaaaaggc
     1261 tccaatggca aaatatcaga aggataatct taaagaggac agttctcaat ttgaaatgct
     1321 aaaaaaatta gaattatctc aatctgattt tattagaata aaagattatt gtatacaaaa
     1381 taaaattgat tttttgtcta ctccttttga tgaagaaagt gcagactttc taaaacaaat
     1441 tggtgtacaa ggatttaaaa ttggctcagg agatttaaca aatttacctt ttcttagaaa
     1501 aatagatatg tatgggttgc cagttttatt atctacaggt atggctgata ttcatgaggt
     1561 cagtgaggct gtagattgtt tcatagatag tccagttact attttacatt gtacttcaaa
     1621 ttatccggct agttctgaag atattaattt attggcgatt aacacattaa aagaaatgtt
     1681 cgggataccc gttgggtact ccgatcattc attgggatat gatgttggta tttgtgctgt
     1741 tgcgatgggt gctaaggtaa ttgagaaaca ttttaccttg gataaggaac tacctggtcc
     1801 agatcataaa gcttctttag atcctcagga atttgcagat tttgtaaagc atattagaaa
     1861 cactgaaata tttttaggtg atggtgttaa gcgagcaatg ccttctgaac ttagtacaaa
     1921 agaagtatca agaaaaagcc tagtggtcac tgagaattta aatgtgggag atattcttac
     1981 agagaaatct cttacaataa aacgtcctgg aaatggaata gctcctaagt ttttatatca
     2041 atatattggc aaaaaagtta aaagaccaat aagtgcagat agtgttctgc ttgaggaaga
     2101 tgttgaatga aaaaaatact tatagtaagt ggaacacgag ctgattatgg aatatataaa
     2161 cctgttgctg agaatttact acaagataat tgggatgtat catttgttgt ttctggaatg
     2221 catttatccc ataaatatgg atttactaaa gaattgatct tagctgacgg atttagaatt
     2281 agtggagaag ttcaaagttt gtttttagag aacaataagg gaaacatggc tcggtctgtg
     2341 gcattagaga taaatggttt cacaaatata tttgaacaaa ctagccctga ttttgtatta
     2401 ttactagggg atagaggaga aatgttggca gctgcgatag cttgtttgta tcttggaatc
     2461 aaaacagcac atattcacgg aggcgaagtg tctggtactg tagatgaatc aataagacat
     2521 gccataacga aaatagcatc tgttcatttt gctgctacgg aaaaaagcaa agagcgttta
     2581 ataaaaatgg gggaagattc ttggagagtt tttcccgtcg gagctcctag aatagatact
     2641 attttaaata at
//
LOCUS       DAMYXM010000149         2631 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.154, whole genome shotgun sequence.
ACCESSION   DAMYXM010000149 DAMYXM010000000
VERSION     DAMYXM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.154"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(61..2424)
                     /locus_tag="PNS21_002819"
     CDS             complement(61..2424)
                     /locus_tag="PNS21_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HDI5160441.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT          700 a          553 c          483 g          895 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt t
//
LOCUS       DAMYXM010000150         2625 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.155, whole genome shotgun sequence.
ACCESSION   DAMYXM010000150 DAMYXM010000000
VERSION     DAMYXM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.155"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..558
                     /locus_tag="PNS21_002820"
     CDS             <1..558
                     /locus_tag="PNS21_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HDI5160442.1"
                     /translation="FEKKKYVLQVFHPVVTELENIEKQILALINSMEQQSQPIICILP
                     NSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTILKECKFMIGNSSSGIIEAASFKI
                     PVINLGTRQNGREQSKNIINANLNENEIETALNKIQDPQFIKSLKNVKNIYGDGNSSR
                     RITKTLNQILNDGEDIKWIQKQIAY"
     gene            531..1208
                     /locus_tag="PNS21_002821"
     CDS             531..1208
                     /locus_tag="PNS21_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDI5160443.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1198..2340
                     /locus_tag="PNS21_002822"
     CDS             1198..2340
                     /locus_tag="PNS21_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDI5160444.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            2353..>2625
                     /locus_tag="PNS21_002823"
     CDS             2353..>2625
                     /locus_tag="PNS21_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDI5160445.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTE"
BASE COUNT          979 a          343 c          512 g          791 t
ORIGIN      
        1 tttgaaaaga aaaaatacgt tttgcaagta tttcatccag ttgtaaccga actagaaaat
       61 atagaaaaac aaattttagc tttgatcaat tcaatggaac aacaatctca accaattatt
      121 tgcattttac ctaatagtga tgctggtagc gaaattatca gaaaaatgta ccagaaatat
      181 ttaaaaacgg atattattta tattacgaat ttagagccgg aggaatacct gactatatta
      241 aaagagtgca aatttatgat cggaaattct tcttctggga taattgaagc tgcatcattc
      301 aaaataccag taataaattt aggaaccaga caaaatggac gagaacaatc taaaaatatt
      361 ataaatgcaa atctaaatga aaatgaaatt gaaactgctt tgaataaaat acaagatcct
      421 cagtttataa aaagcttgaa aaatgttaag aacatctacg gtgacggcaa cagttctaga
      481 agaataacaa aaacattgaa tcaaatattg aatgatgggg aagatattaa gtggattcaa
      541 aaacaaatag cttattaaaa tatactagtg atccatacat tattattggg gctggcggac
      601 acgcaaaagt cattattgaa accttaagat tagaaaataa gttaatatat ggattttttg
      661 atgataagca aaataaaatg caagatctga aatatcttgg gaaaataaac gaattgaata
      721 gctgggcgga tagcaaaaaa aaaatctata aatttattgt agctattggt gactgtgtag
      781 taagaaaaaa gattataaaa aaattgaata tgccaagtga gcaatatgga atagctgttc
      841 atccaagtgc tatagtggca aaagaaattt caattggtta tggaactgtt atttttgcga
      901 atgctgtaat taacccagat gctgtaattg gagagcatgc tataataaac acaggtagca
      961 ttgttgagca tgattgtaga attaataact atgttcattt atctccaggt gtatgcttag
     1021 cgggtggagt acatgtaggt gtaggaactc aaataggaat tggttctcaa tgtatccaga
     1081 tgaagaaaat tggatcttgg tgtatgatag gcgcagggag cactattgtt aaggatattc
     1141 cctcacattc cttagcttat ggcaatccag ctaaaattaa gaaagaagga attaattttg
     1201 aattctagaa ttttattatc aagtcctcat atgggaggaa cagaagaaaa atatgttgct
     1261 aaggctttta aagataattg gatagcgcca ttaggtgaaa atgtggataa atttgaagaa
     1321 agtattacaa aatatactgg ttctaagggc ggagcttctg cgttaagttc aggtacagct
     1381 gccatacatc ttgccttaag actgcttaat gttggaaaaa acgatattgt ctttgctcct
     1441 tcatttactt ttatagctac agtaaatcct attttatatc aaggggctgt accagtgttc
     1501 attgactcag aacctaatac atggaatatg agccctgttg cactagagag cgcattagaa
     1561 gaagctgaaa gaaacaacca attacctaag gcaatcatcg tagtgaattt atatggtcaa
     1621 atggctgatt atgaacgaat tttagctatt gcaaaccaat ttaatgttcc agtgatagaa
     1681 gatgcagcgg aatcgttagg ttctacttat aagggaattt cgtcaggctt atttgggaca
     1741 attgggatct attcatttaa cggtaataag atcatcacaa cctcaggtgg aggagctcta
     1801 gtgtctagtg ataaaaaata tgttgagcat tcaagatttt tagcaacaca agcaagagat
     1861 caagcaccgt attatcaaca tagcgaaata ggttataatt accgtctaag caatatttct
     1921 gccggtatcg gacgtggaca aatggaagta ttgaatgata gagtagctaa acgaagagaa
     1981 attttcaatc gatataagga agctttttct ggaattgata gaattgaaat gatgccagaa
     2041 gtggatggca tggtgccaaa tcgttggttg tctactttaa cgatcaatga ttataattct
     2101 attgatcaag tagttaacgt aataaataaa ctaaatgaaa aaaacattga aacaagagct
     2161 ttgtggaaac cactacattt gcaaccagta tttcaagggg taaaaatgta ttaccatgat
     2221 aataatctta gtgtgtcaga aaagttattt aatagcggct tatgcttacc ttctggaagt
     2281 aatatgacag atagtgaaca agaatttgtt attgatgagt ttaaaaaaat attcaagtag
     2341 gtgtgatatt ttatgaacat aggaattatt ggtttaggtt ccatggggaa aagaagactt
     2401 agactcattc aagaacactt taatgatatt ttactatgtg gtgttgatac taatgaggcg
     2461 agacgaaagg aagttgaaaa ggaatttgga ataccaactt ataatagtct aacagaggca
     2521 aaaaatttgt ttgaaataaa ttcagttttt gtttgtacat cccctataac ccatgaattt
     2581 attataaaag aggctttaaa tttagattgt aatatcttta cggaa
//
LOCUS       DAMYXM010000151         2526 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.156, whole genome shotgun sequence.
ACCESSION   DAMYXM010000151 DAMYXM010000000
VERSION     DAMYXM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.156"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..345
                     /locus_tag="PNS21_002824"
     CDS             <1..345
                     /locus_tag="PNS21_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI5160446.1"
                     /translation="NKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDA
                     PIILADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKK
                     LKKVNKMNLEVE"
     gene            345..1721
                     /locus_tag="PNS21_002825"
     CDS             345..1721
                     /locus_tag="PNS21_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5160447.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2007..>2526)
                     /locus_tag="PNS21_002826"
     CDS             complement(2007..>2526)
                     /locus_tag="PNS21_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI5160448.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIV
                     MFLWLFRMGVIP"
BASE COUNT          937 a          352 c          457 g          780 t
ORIGIN      
        1 aataaatcag cagatgaaag tatcttgttt gaactaggtt tagataaaaa acaaataaaa
       61 agaaatgtta tgaaattatc tggtggacag caacaaagag tagctattgc tagagcattg
      121 gtatcagatg ctccaataat actagctgat gagcctactg gtaatctaga cagtgttact
      181 gctggagaaa taattaatat attaaagaca ttagctaaag atagaaataa atgtgtaata
      241 gttgtaacac atagtaagga agtagcagat tctgcggata tcattttaga actaagtggt
      301 aaaaagttga aaaaagtaaa taaaatgaat ttggaggttg aataatgata aaaaatgcat
      361 ttgcttatgt aactagaaaa agtttaaaat cattaattat catattagtt attttatcta
      421 tgtcaacttt aagtctcatt agtttatcta ttaaagatgc tacggacaga gcttcaaaag
      481 aaacatttgc taatataaca aatagttttt ctatggagat aaatagacaa gtaaatccag
      541 gaacacctag aggtggggga aatgtaaaag gtgaagatat taaaaagata tctcaaacag
      601 atagtataga ctcctatgta aaaagaataa acagtgttgc agatttagtt gattatgata
      661 ttattgaaac tcaagagact cttgcgaatc aatcacctga aagagcaaag aattttaaaa
      721 gaacagttat gttaactggg gttaacgact caacaaaaga aacaaaattt gtatcagaag
      781 catataaatt agtagaagga aagcatttag aaaatgaaga taaaaataaa atcttaatgc
      841 ataaagattt agctgaaaaa aataacctta aagttggaga taagataaaa ataaaatcta
      901 atttatttga tgctgataat gagaaaggtg cagatgaaac agtagaagta gaaattaaag
      961 gtctattcga tgggcataat agcggtgtag taagtgcagc acaagaacta tacgaaaata
     1021 cattaatcac tgatattcat acagctgcta aagtttatgg taataccgaa gatacagctg
     1081 tataccaaga tgcaacattc tttgtaaaag gtgataagaa tcttgatagt gtaataaaag
     1141 atcttgggaa attagatata aattggagag aatataattt gattaaaagt tcatcaaatt
     1201 accctgcatt acaacaatct atatcaggta tttactcaat ttcaaataaa ttattttttg
     1261 gctcattaat atttgcagga gttgtagttt cattattatt attcttatgg atgaatgcta
     1321 gaaagaaaga aatagcagta ttactatcat taggtatatc aaaattagaa atttttggtc
     1381 aattcctaat tgagatggta tttatatcaa tccccgcatt cgttggttcc tatttcttag
     1441 ctcaatatac agctgacaag cttgggaata atatattgaa taaggttact ggtgatatag
     1501 ctaaacaaat agctaaacaa tctgcatcta gccaattagg tggtggggca gaagctgaag
     1561 gatttaacaa gacattatca agtttagata tcaatgtatt acctaaattc atgatttatg
     1621 tagttatatt tatgagttta gtacttcttg tatcattgat tatttcttca ttcaatatat
     1681 taagaaggaa tccaaaagaa ttattaattg acaataatta aaattttcgg tgctttttag
     1741 caccaaaaat gagagtgtaa tataaaccgt gtaagtaacc atcaatccta ggcgggtggt
     1801 tgcttcacgg tttttatttt tctaaaataa gatcaagagg tgattacacg atggctagaa
     1861 agaaaagaaa tcctgatgct gagaagttag ctgaatccat tttgaatact tatcaacccg
     1921 aatctgtaga ggatatgcaa gatgttttaa aagatgtgtt tggtccctta tttgaaaaaa
     1981 tgcttcaagg agaattgaat aaccatttag ggtatgacgc ccattctaaa gagccaaaga
     2041 aacatgacaa tcgacgaaac ggttatggca ataagacgct taaaacgagc tttggtaaag
     2101 taaacattga tgttcccaga gaccgggagg cttcttttga acctgagttg attcctaata
     2161 gaaaacgaga tgtctccgac atcgcagact gtagacaaaa gctgttccaa actcgtaact
     2221 cgagcttgga acagcttttt atctacttcg tttcgaattt ttgaacaaaa aaataaaggc
     2281 tccttgcctt tccatagcca ggtggctaat ttctttaaat tcattgcagc aaacactagc
     2341 atcgtatgcg tcgcgacttt ttccaagcct cgatacttgg tccaacgcat cccatgcttt
     2401 tcttttgcat ccgcaaatat tcgttcaatc gtttgtttcc gttttttgta aattgattta
     2461 ttcagatctg ttaggcgaag atgttccact tcatccatca gatctcccca aacatgtctc
     2521 gtgatg
//
LOCUS       DAMYXM010000152         2397 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.157, whole genome shotgun sequence.
ACCESSION   DAMYXM010000152 DAMYXM010000000
VERSION     DAMYXM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.157"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            206..2068
                     /locus_tag="PNS21_002827"
     CDS             206..2068
                     /locus_tag="PNS21_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDI5160449.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2397
                     /locus_tag="PNS21_002828"
     CDS             2068..>2397
                     /locus_tag="PNS21_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDI5160450.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          864 a          353 c          466 g          714 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataat
//
LOCUS       DAMYXM010000153         2189 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.158, whole genome shotgun sequence.
ACCESSION   DAMYXM010000153 DAMYXM010000000
VERSION     DAMYXM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.158"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(325..528)
                     /locus_tag="PNS21_002829"
     CDS             complement(325..528)
                     /locus_tag="PNS21_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160451.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(987..1952)
                     /locus_tag="PNS21_002830"
     CDS             complement(987..1952)
                     /locus_tag="PNS21_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDI5160452.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          629 a          506 c          346 g          708 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggctttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cgcgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgagggt tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cttaaacatt gacgtaaatt tactctcact tatctgactt ttttactcgg tatcactccg
      721 agtaagccgt catattcatt gggataatct cgtctgcttc gctctaattt tccagagtga
      781 ggggtaactc cttcccccga attaccccaa agagtgacac tgggatatcc ctaaatttat
      841 tataaaaagc caagtctctt tgcgcaactg ttttatccaa gtcggcattt gcggattcgt
      901 tagcgttttt tcgctcttgc tgcaaacgca cgacccacca tgccgacttg tgctgaaaat
      961 aaaaaagtgg acaaaaattc atccactcat tcttccgtca cgtccaaccc caaaagccac
     1021 gtatccaatt cttttttcaa aacatctgcc gattccgaaa atgtctctcg taaatgatta
     1081 actaactcat cactttggct ttcttttttc aacaaatttc gatatttata ttttgtatga
     1141 cgagccaagc cagcccataa attttgatct gaaatcaatc ctgcaatcat aattctatca
     1201 gtatgctttg tatcagtcgg caaaaaatga ggacttgcga aactgtctaa actttcatga
     1261 accatcgcat accaatcagt agcttttcca cgccgtaact gcaattccag tcgccaccaa
     1321 gtttttattt ccggtggtac aattttccgt ttggtttctt gttccaattt tttgttgtac
     1381 atccttattt gtcgctcact ggcacgacta ccccaatatg ccgtttccag cttcccagac
     1441 cgcccataga ttggtttgaa tgaaacaggg tcaaccacac gatattgcgt aataaaatca
     1501 tcaggcacat cgataatgtc gcaagcaata tcagcacgtg agaaatgtgg ttctaaaaat
     1561 aaatcatgga taaagttctt catcgaaccc gccaaaaatt ggctaatttt atttgggtta
     1621 aagtcaattc gcccttgccc ttcttgatat ttcaaaattt caatgtaagc aatattctca
     1681 tcatgcttat ccacaatttg ccaaccattt agaccaacag atttaacata accattattt
     1741 tcattcagtc gcattaactg ttcaaagtct aaaatcaata catcattttg cgtgtgataa
     1801 taaatattct ctttgagttt tccaacaatg gtaattcggt caattgacca ctgtatttta
     1861 aggggggtgg tcaaagtttg cacacttaac cgcctgttag atagggctgt taaattttcc
     1921 cactcattta tgatcgtgcc acgtttactc aaattttgtt cctcctcaaa gaaaaagaga
     1981 cttttggaaa gaatttccag agttcacgta accctaaaaa ttcatatcca aaaatctcta
     2041 gcttgaagaa acctaaaaat attcgtataa tgaacctgag ttacggagct aggtgaagta
     2101 gtttccgtaa atagtcgaga gtgttagcgc actgctcggc ttttattttg ctcttttttc
     2161 tcgttttctt tgattatagc actcctcct
//
LOCUS       DAMYXM010000154         2180 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.159, whole genome shotgun sequence.
ACCESSION   DAMYXM010000154 DAMYXM010000000
VERSION     DAMYXM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.159"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            700..>2180
                     /gene="ltrA"
                     /locus_tag="PNS21_002831"
     CDS             700..>2180
                     /gene="ltrA"
                     /locus_tag="PNS21_002831"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDI5160453.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKP"
BASE COUNT          858 a          336 c          455 g          531 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc
//
LOCUS       DAMYXM010000155         2147 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.160, whole genome shotgun sequence.
ACCESSION   DAMYXM010000155 DAMYXM010000000
VERSION     DAMYXM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.160"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(119..709)
                     /locus_tag="PNS21_002832"
     CDS             complement(119..709)
                     /locus_tag="PNS21_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDI5160454.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1232..1393
                     /locus_tag="PNS21_002833"
     CDS             1232..1393
                     /locus_tag="PNS21_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDI5160455.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1412..1906
                     /locus_tag="PNS21_002834"
     CDS             1412..1906
                     /locus_tag="PNS21_002834"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDI5160456.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
     gene            1899..2060
                     /locus_tag="PNS21_002835"
     CDS             1899..2060
                     /locus_tag="PNS21_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160457.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          775 a          333 c          364 g          675 t
ORIGIN      
        1 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
       61 ccatcagtaa aattacttgt catactattg ataaggagta tttaattgaa gagaatgatt
      121 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
      181 tctttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
      241 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
      301 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
      361 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
      421 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
      481 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
      541 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
      601 tagaagcagt tttgttttgc catccctctt tttggtttag tgtgctagta atataagcac
      661 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
      721 cctttactat ctatgacaag gacaacagtt ttaacataca gacattcatt aaatttggat
      781 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagaca gtcaacttaa
      841 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
      901 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tcttaaaaga
      961 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
     1021 tgaaaacaac cctagaaata tataactagt ccaatgaaaa aaaagcgaat gccgtagaaa
     1081 acaaggattc tgcgtacatt catatctcat tttgcgagca tatctgctat tactgtgact
     1141 aaatgttaat atggttaaaa tgaccgttat attttttata taaatatcgt ataattagta
     1201 tgaatataga agaaggaaag aaggtgcaat cttggaacct aaagacaagt atttagatta
     1261 cgctagggtc aataaagata aatttattga aacaatcatc aagggaaaaa tccctgatga
     1321 tgaaaaagat gcggtattca tggctggaag cccaggagca ggtaaaacag aggttgcact
     1381 aggtttagct taaaattatg acaaccatgt tgtgattgat gcggactatt ttagagcaca
     1441 atttccagaa tacaatggaa agaactcaag tgtttttcag aaagcttctt cttggttagt
     1501 tgaacaatca ttaaaatatg tactggaaca tggctattct ttcatactag aggatacttt
     1561 tgctatcctt agtgcagaga aaaatattat tcgagccctt aaaaataact ttcgcgttac
     1621 gattttctat gtttaccaag atcccaaagt tgcctgggat ttcactcgaa aacgtgaact
     1681 agctgaagga cgtcatgtgc caaaagaaat gtttatcaat gcgtttttta agtccagaga
     1741 gaatattgaa aaagtcaaaa atagacatcc tgaagtagtc cttcacataa tgattaagga
     1801 ttatcaaaac gacatttctg aagtgcatta tgcagcagat aatattcaat tagtactacc
     1861 aatacaatat acttcaaaag agttggagga ggaattacat gactgagaaa gaacaagtac
     1921 aacaaatcgt gaagaagtat aataaaagta tcgctgattt atcagaaaac gcctcagcga
     1981 aagaatttaa aacagtaatg aattacgtag cagatgaagc aaatcgtaag cagcgaaaac
     2041 tagttgggtt ggataaataa attttttaaa ccatatcctt gtaaagttga gttttttatt
     2101 ttaatgaatt aagactatta aatagataat tattgtaact taaaaaa
//
LOCUS       DAMYXM010000156         2133 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.161, whole genome shotgun sequence.
ACCESSION   DAMYXM010000156 DAMYXM010000000
VERSION     DAMYXM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.161"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..299
                     /locus_tag="PNS21_002836"
     CDS             <1..299
                     /locus_tag="PNS21_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160458.1"
                     /translation="IDQQTGVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTP
                     VSDKNGEFLKDVWIYPKSEASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            312..572
                     /locus_tag="PNS21_002837"
     CDS             312..572
                     /locus_tag="PNS21_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160459.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            569..>2133
                     /locus_tag="PNS21_002838"
     CDS             569..>2133
                     /locus_tag="PNS21_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDI5160460.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTP"
BASE COUNT          783 a          340 c          401 g          609 t
ORIGIN      
        1 caattgatca acaaacaggt gtctttgaat tctcggtaga agtacaggca aatcagaaac
       61 aagcttatta tattgtcaat gaatcaagtc ctgaaaacat ttctaatagc gaggatattt
      121 tgctattaac acctgtttct gataaaaatg gtgaattctt aaaggatgtg tggatttatc
      181 ctaaatcaga agcttcgcaa cctaaagaag aagtaaaaaa aatcgtatct actggcgtaa
      241 agaaaaactt ctttgagaat tgttgggatt ttgttactca cttgttttct agagattaag
      301 gaagaaataa aatgaaactg atcaaattat tattattcaa tttttcttgt ttaaccgcag
      361 tctttttgta ctgcggttta ttattttcta aactatctat tttgccaagc taccaactat
      421 acctaatgag gattttaaac ataccagctg cttccatgcc gctagatatt atggaaaagc
      481 tcattaggaa tccattattt actacgatca atttgatcgt tattgtcgga atttcactag
      541 tcatttataa aaaagagggg atgaagggat gaattttaaa cactcgtgga caagacggtt
      601 aaaatatgtt tttcccataa tgatgatgtt aggtacattg tttgggttaa aaacagccaa
      661 tgtctttgct gaaaaagtta ttgtcgatcc gtcaaatcca atccagaacg taaagaataa
      721 ttttattatt ccaaaagatg ttcctaatgc aaaaacaaat attactgtaa acggagcgtc
      781 aaaagtttac aaatatcatg ttgatgccaa caaaggtggt tttacagacg actacgtagg
      841 attagtggag ggaaacaaaa atatacgcta tccttctttc caaaaagaat acggtgaaac
      901 aaaccttgtt ttagaaggcg taacaaccaa caccaaagtc aatattgact atggaaaaat
      961 cggtacgtat aacgggaaaa aagtcaatat caagctagtt ctatctaata ttcacttata
     1021 ctcggatacg ttgccgtgga atctactaga taacaactat acaaaaactc attttcgaga
     1081 cgatggctat aaaaatacga atggtgcaat gagtaaatct aaaaaaagaa cagtactttg
     1141 gatctcagac aatcttttct caggaattgt ttaccattca acacaaatga atgtgcagct
     1201 agtagccaca tatgaggacg gtagccccgt tcaattttca ggagatacat ttatcagttt
     1261 taactctttg aaccctgcag gaggaaaaag cacggatctt aaaggagaat atgcccacta
     1321 tgacaaaatg aatacgacag attggtatgt cagaaaagat acggttcttt cagaatttaa
     1381 atctttctac aacaatctga acgtggtagg tggccaccca ggaggaagct ctaagctgac
     1441 acaagcggat aatgatttca ataatttgca tgataaatta ggagatccga agtttggtca
     1501 aggtactgtg tcctttaaaa tatctgaagc aaatccgacg tttgtgattg gttcttctaa
     1561 tgttcaaacc tggtttactt tatcttctgc gacaatcttt agtgttgttc ctgatcaacc
     1621 tgaaaaaaca ggggttgata aaaatggaaa taacgtgaac gataaaatgt tgcaagtagg
     1681 agacacgatt caatatcgaa tcaaacagaa ggtgaatcga ttaggagtgg atttactagc
     1741 ggtttacgat cgatttgagt taatcgataa tttaccaaaa gaagtaaatt acgtcgatgc
     1801 acacgttgaa agtggtacaa ataaaaaatt cgatgtatct ggtgaagtga cctatgacaa
     1861 aacaaaacac caggtaaaat atgcagcgaa agctgataca ttaaaaaatc gaatgaagta
     1921 taacggcgaa acttacgaat tagtgatcaa tgtaaaagtc aatgaattgg ccaatcaaaa
     1981 tagtgtagcc aaaaatcagg gaacgtcgat cattaataaa gtagaaaagg atacaaatat
     2041 cattaccgtt tatttcccta aaattccagt gaaagaagta caacaaaatg gaaaagatgt
     2101 aaacggacga aatgacggta agaagggtac gcc
//
LOCUS       DAMYXM010000157         2058 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.162, whole genome shotgun sequence.
ACCESSION   DAMYXM010000157 DAMYXM010000000
VERSION     DAMYXM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.162"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..267
                     /locus_tag="PNS21_002839"
     CDS             <1..267
                     /locus_tag="PNS21_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDI5160461.1"
                     /translation="KIIEIHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVK
                     EMPESANDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            268..1020
                     /locus_tag="PNS21_002840"
     CDS             268..1020
                     /locus_tag="PNS21_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDI5160462.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            1025..1972
                     /locus_tag="PNS21_002841"
     CDS             1025..1972
                     /locus_tag="PNS21_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDI5160463.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          724 a          271 c          403 g          660 t
ORIGIN      
        1 aaaattatag aaattcattt agattatttt ggaagagttc ctacaagaaa atttcaattg
       61 tttactttag atgaatgtat caatgttgac ttattaaata ataaaataag ttttgaagaa
      121 aacattgtta aagaaatgcc tgaatctgct aatgatatgt atgagaagga gttgaaatat
      181 ttctttgaaa acgtagtaac aggcaaagaa aactggaatg atttatatca tgcaaacgaa
      241 gtattgaaaa tagctgagga gaaataaatg gaaattttat ttactatttg tggtagagct
      301 gggtctaagg ggtttaaaaa taaaaatttg aaatatttgg caggaaagcc cctagtccat
      361 ttcgctttag caatgataga tatctatcaa caaaaacatc ctgaacataa ttgcacgact
      421 tgtttgaata cggacagtaa tgagttagta gaattgacga ctaagttaaa tccgtctgtg
      481 aaaattgtag aacgtaaacc tgaattagct acagatactg tagggaaaat tgaagtgata
      541 cgtgattcat tcttacaact agaaagagaa gataatatat ttgatttaat tatagattta
      601 gatttgactt cacctttacg tgaagtttct aatcttgaag aattaataga tgaacatcta
      661 aaaaatttag ataaagacgt ctacttttca gttgtttctg caagacgtaa tccctatttt
      721 aatatggtaa aaagaattga aaatgaagtt actatagtta ataaatcaag atacacatct
      781 aggcaacaga cacctgaagt ttttgaaatg aatgcctcaa tgtattcttt taaacctgaa
      841 tttctaaaaa aaagtaatta tatttttgat gggcaatgtg ggataataaa aatgagagat
      901 tatctaattt tagatattga tagtgaagaa gattttaaat ggttagaatt tttgtttcct
      961 aaatttttag aagattcttc ggagcttcaa gatatctata attatttata tcggaggtaa
     1021 aaggatgagc tgttatctta tcaccggtgg ggctggcttc attggttcta ccttagccaa
     1081 tcatttagga gaagaacatg tggtaatcgt cgtcgatgac ttatcaatgg gaaaagtaga
     1141 aaatcttgat atgacgaaaa atatcacttt tattgaggga gatgtcgctg agaaatcctt
     1201 gatggaagag atcatgaaag ctaataaatt tgattatatc tttcatttag cagcagttgc
     1261 tagtgtggca gattcagttg cccgaccagt agaaacacat cgtgtgaatt ttgaaagtgt
     1321 attaatgatt ttagaattga tccgcaagta tcaacctgat ttaaaacgga tcgtgtttag
     1381 ttcttcagcg gctgtttatg gggatgaacc gactttacca aaaaaagaag agtccgtgat
     1441 tcgcccattg acgccttatg cggttgataa attcgctgca gaacaatatg tgctagatta
     1501 ttgtcattta tatgatgtac ccggtagcgc agttcgtttt ttcaatgtct atggtccaaa
     1561 tcaaaatccg aattctccat acagtggtgt gatttctatt ctcgtagatc gctataagaa
     1621 acaaatcgta ggagaagcaa cttcatttac attatttggt gatggaagcc agtctagaga
     1681 ctttgtttat atagatgatg taatccaagc attactttta gttgcgaatg aggaagctgc
     1741 actggggaaa caattcaatg ttggaacggg taaagcaacc acacttttgg aattgattca
     1801 tgcaatcaat gaaattctcg gtgtagaatt gacacttgaa tacaaagaag agcgttcggg
     1861 tgatatccat gactctctag cagatattac taagatccag tcaattggct atcaaccgaa
     1921 atatggtatt ttaagtggaa tgactaatta tttgaaaact gaaatcaatt aaaacatact
     1981 aagataagat tagagggtaa attcaggctc tttgtcaata aggactgatg agttttgtaa
     2041 aatcaaatct gggataga
//
LOCUS       DAMYXM010000158         2001 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.163, whole genome shotgun sequence.
ACCESSION   DAMYXM010000158 DAMYXM010000000
VERSION     DAMYXM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.163"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..376
                     /locus_tag="PNS21_002842"
     CDS             <1..376
                     /locus_tag="PNS21_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDI5160464.1"
                     /translation="NVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRR
                     FPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDD
                     WQKVMAAEVIATYLDGDLVYKK"
     gene            424..1824
                     /gene="yfcC"
                     /locus_tag="PNS21_002843"
     CDS             424..1824
                     /gene="yfcC"
                     /locus_tag="PNS21_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDI5160465.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1857..>2001
                     /locus_tag="PNS21_002844"
     CDS             1857..>2001
                     /locus_tag="PNS21_002844"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160466.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          553 a          339 c          432 g          677 t
ORIGIN      
        1 aaatgtttcc acagaggact tctttaatta ttgtcgatta gaggaaagtg gtgtaacagt
       61 cactagcggt acagatttac cgctgtttat tactgatatt cctgaatcga ttataagagc
      121 agtattccgt cgttttcctg aaggagaaga agtctgggag aaagaaacaa gcttcacgac
      181 aaccgagctg ctgaaaagtt ttacaagcaa cagcttcaaa gtaaatggtt tatcggagta
      241 cggagaaata aaagtaggga aaagcgccag cttagctgtt ttcagcgagg acctctgtac
      301 tgatgactgg caaaaagtga tggctgcgga agttatcgct acctatcttg atggtgactt
      361 agtttacaag aaataatgct tttcgatata acgttataaa acgaattaaa gagaggttaa
      421 aaaatggaaa caatgaaaag aaaattcacc ttcccacata gttatacatt gatttttatc
      481 ttaattattt tggctgcaat tttaacttgg ataattccta gtggtcagtt tgagacagtt
      541 gttgaaaacg tcgcaggtat ggaaaaaaca acggttgttc cgggaaccta tcaccaaatc
      601 gataaggcag attatggaca aggtttttcc tctatactaa tggcaccagc tgaagggatt
      661 atttcagctg ttgaggttgt tgcttttgtt ctcatcgttg gtggtgcctt cgggattatc
      721 ctaaaaacgg gagccatcga tcgagggctg tttaccttag caaaagttct gggagacaag
      781 gggatcttag ttattccgat gtcgatgatt ctattcagct taggaggatc aacctttggc
      841 atgagcgagg aagtcattcc cttatttgcg gtgtttattt ctctgatgtt ttctcttggc
      901 tatgattcca tgactgcgat cttgattcta tttttaagta cacaagcggg atatgttggg
      961 tcgacgatta atcccttcaa cgttttaatc gcacagggag tagctggcgt ttacggaaat
     1021 cccctgcttt ggtttcggat gctatcctgg attgtcatta ctgggatttc aattgttttc
     1081 accatgcttt atgctagcag agtcaaaaag aatccactgt cctctattgt ttatgagagt
     1141 gatttaaagg ttaagcataa gtttcaaaat tttgacactg aaaaagagtt ttcaacacaa
     1201 gataaactaa ttatcgccgg ttttatcgta ggtttaggaa tcatgatttg gggtatcatt
     1261 tccaagggat ggtatatggt tgaaatttct gcactattct taggattagg tctttttgct
     1321 gggggcatcg ctagattttc tcaaaaggaa attgctgaag cttttgtcga aggctgttca
     1381 gatttcgcct atgctgctgt aatcattggg ctagctagag gagtcttggt aatcttggaa
     1441 ggtggaatga ttattgacac tattctaaac agcttggcta accttttagg tggtcttcca
     1501 aaattcctgt tcactacgat tctactcatt tctcaagtaa ttgtttcctt ttttgttcca
     1561 tcctcctcag gagctgcggc attaacgatg ccggttatgg caccattggg tgatctaatg
     1621 aatgtaagtc gtgatgttgt tgttctaagt aaccagtttg gaaatggttt aatgaatatt
     1681 atctccccaa ctagtggtgt attactggca ggattagcca tcgcagagat taatttctct
     1741 aaatggttta aggttggctt caaaatattt gccatcttaa cccttgtttc tgctgttttg
     1801 ttatacattg ctacactctt ttagtcttaa tcatccatta ataggaggca atagttatga
     1861 ttaaaataaa tcaattagca ggaatgaata tccactattt attttattct ctcaaagaat
     1921 ttttcgctta tcaacagaaa attggtattt cttctgttga actttgggga ggtgctccgc
     1981 attttttcat agatgctttt a
//
LOCUS       DAMYXM010000159         1993 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.164, whole genome shotgun sequence.
ACCESSION   DAMYXM010000159 DAMYXM010000000
VERSION     DAMYXM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.164"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="PNS21_002845"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="PNS21_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDI5160467.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="PNS21_002846"
     CDS             852..1040
                     /locus_tag="PNS21_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDI5160468.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="PNS21_002847"
     CDS             1053..1610
                     /locus_tag="PNS21_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDI5160469.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="PNS21_002848"
     CDS             1642..1884
                     /locus_tag="PNS21_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDI5160470.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          308 c          406 g          555 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc ctt
//
LOCUS       DAMYXM010000160         1953 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.165, whole genome shotgun sequence.
ACCESSION   DAMYXM010000160 DAMYXM010000000
VERSION     DAMYXM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.165"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            196..1275
                     /locus_tag="PNS21_002849"
     CDS             196..1275
                     /locus_tag="PNS21_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDI5160471.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(1472..1903)
                     /locus_tag="PNS21_002850"
     CDS             complement(1472..1903)
                     /locus_tag="PNS21_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HDI5160472.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          566 a          436 c          443 g          508 t
ORIGIN      
        1 ttttttagga atgtaaagtc ttatctcttt tcttaattgt acaattagta ttatctgcac
       61 tactttctaa ttaaataaat ttttagttag gcggatttta taattaactt agccatttgg
      121 aaataaaaaa acgccacgtg aattccatgt tatattgaag ttaccacaac tctcaataga
      181 caaggatgaa ttcacatgac gcaaaaccat gataacacag tttcacgcaa gggaaaacac
      241 ctaacctact cagaacgttg ccaaatcgcc atcctgaaaa aggaaaacta ttccaaccgt
      301 caggttgcgg atgtcctggg ctgcgtgccg cagaccataa acaacgaaat caaacgcggg
      361 accgttactc agcttaaccg ccagaatcag aacgggaagg tctacgacta ttatcatgac
      421 gtctacgacg ccgatgccgg gcaggcggcc tacgagaaga accgcttgaa ctgcggcaga
      481 cggcccttat gggccgacac ggacgccttc attgaatggg cggacgacat gatgctgttg
      541 gaaaaatggt ccccggacgt ggtggtgggc tttgccagaa aacacgaatt gttcgacccg
      601 gccattatcc ctgggaccac caccctctat cagtggatcg accgtggcat catgaaaacg
      661 aagaatatgg acctgctaga aaaactgtcc cgcaaaccga aatcgtcccg tcatggcaac
      721 cgcccgaata aacgggtgct agggaaatcc attgaagagc gcccggaggc ggttggtacc
      781 cggaaaacct tcggccattg ggaagtcgac acggtcgtag gaaacaagac caagtcggat
      841 gccgtgctgc tgacgttggt ggaacggcag acacgcttcg aggtcattct gaagctggcc
      901 gggaaggatg ccgaatcggt caaccaagcg gtccgacagc tgagagaacg ggctggtgcg
      961 tacttcggcc agctgttcaa gacggttacc tcggacaacg gctcggagtt cgcgggactg
     1021 cacgatgcct tgcaggatgt ggcaaacatc tacttcagcc acccctatgc ttcctgggag
     1081 cgcgggacga gcgaaaacca acacaagctg attcgccgtt tcatccccaa gggcagccca
     1141 atcggcaacg tgagcgaaaa ccaaatcttg cggattcagc gttggatgaa cgattacccg
     1201 cgaaagattt tgggctatgc aacgccgcat gacacgttcg tccaggcatt taggcaggaa
     1261 cggttgacgg cttgacacgt gggcccttga caacgagcct ccctcggcat ggcttgtgtc
     1321 tggaaagctg gccggggcca gccagtcggg agactaccat gcctcagctc attgtcaagg
     1381 gcccactcga ttagaaaaat tagacggttg gtggtaacga gcaaccgggt caccatgggt
     1441 ggctaactta aacttgaaat ttacgaaatt tttagttatt aacttgatta aaatcagaga
     1501 taatatcatt taattcagtt tgaagggttg taacgattaa atggaaattt tcttcatctg
     1561 ttttattata tggggatgtt ttcgttatat ataacaactt ttctttttca attttcatta
     1621 ataactctgt cgaagtctgt actttatctt gataattaaa tgcagacatc ccactttgta
     1681 taattgtaat tattgctgaa gcaattgaaa caatcaatag taaagacctg tgttcggaag
     1741 cctgatcaat taatattggg atactagctg ataagactat ttttataata tttccgatac
     1801 caattactct attataaaat cgaatttctt tttttaatct atcgattgta gaatcaatag
     1861 attcgataaa cgctttttga tccatcattt tctctccaat cattttatta aaatcattaa
     1921 caaaaatatt ataataggat ttaagtaagt tct
//
LOCUS       DAMYXM010000161         1949 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.166, whole genome shotgun sequence.
ACCESSION   DAMYXM010000161 DAMYXM010000000
VERSION     DAMYXM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.166"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            192..629
                     /locus_tag="PNS21_002851"
                     /pseudo
     CDS             192..629
                     /locus_tag="PNS21_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(706..>1949)
                     /locus_tag="PNS21_002852"
     CDS             complement(706..>1949)
                     /locus_tag="PNS21_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDI5160473.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          590 a          402 c          356 g          601 t
ORIGIN      
        1 cttgattgac atttatttac aaaaaatatt gccatttaaa aagaaaagtg acacatacta
       61 tttcctccat cattagtttt tgtctatata aggtataccc taactagaca cgaccacaac
      121 attgaaaaat ctggttttat cgtgtatgat tagggtgtat tttaaatagt tgcagaaagt
      181 aggtaaaaat tttgagagtt gcatacatac gtgtctcatc catcgatcaa aatgaacaac
      241 gccagattga agaaatgaaa aaattcggtg cagtgagaat tttcatcgag aaacaatcgg
      301 gcgcaacgat tactcatcga cctgttttcc aagaagcatt ggatttcgtc cgagatcaag
      361 acatttttat tgtggaagct attgatcgac ttggaagaaa ttacgacgag atcatcgctt
      421 cagtcaatta cctaaagaag aaaaacgtac agctaatcat caccagtcta ccgatcatgg
      481 ctgaagcaat tggcaaccct ctcctggata aattcattaa ggatctgatc atccaaattt
      541 tggccatgat cgctgaacaa gagcgtaccg aatctaaacg acgccaggcg caaggcatta
      601 aaattgcgaa agcaaatgga gtttataaag taagcgcccc atagaacagt acctgtacgt
      661 caaataaccc gacctataga atagaatcgg gaatgtttgg cgtttttagt tacgagaaaa
      721 atgttgttgt acatccttgt tccaaggcaa cagttgatct aaagtttctg ctgaaagtac
      781 gggaagattt ggtatcttat caaatagaaa ctctagatac tttacaggat tcaatccgtt
      841 aagttctgct gttttatata cactcagaat gattcctgaa gttcgtgctc cttcaagact
      901 tgtggaatgc aaccaatttt tccgtcctat caccagctct ttgatatttc gctccgcacg
      961 attatttgtg agttctaaac gaccatcttc aaaaataggt tcaaaaccac ttcgtagttt
     1021 acacgcatat tgaacggctg catccaactt ggattttgca acaggatcta tcgttgccat
     1081 ccactcataa aaagcaataa ataatggacg cagttcttgt tgccgacagc ttaatcgctt
     1141 ctcaggagaa aagtctttcc atttcttttc taggacaaac catttatcca attgtttgac
     1201 tgcttgagct gcgggaatat ccgtgttttt ctttcctttt ggaattgctt cgaaaaattt
     1261 ccgccgaaca tgcgcgaaac aatagacaag tcgtacactt tccacggcat tatacccgct
     1321 atagccgtca cagtgtaaat accctgtaaa ctccttgaga aaagttttcg gaacatctcc
     1381 agcacgactt tcgtcgtggt gataataaac gatgggatgc tgactgtctt ttcctgtaca
     1441 aaactgccag taataggtat ctgttttggc actttcaatc acacgataac ttgtttcatc
     1501 cccgtgtaga atctcttctt gattaagata gactcttaat cgttctccta aagggtctaa
     1561 cgcgttttgc actgctttaa tatgccaatt ggtgatatta tctctcgaaa tatccaaccc
     1621 atgtgtttgt ttccaatagt tctcttgccg ataggcagga actttctgac caaatttcat
     1681 ccggatcgtt tccgccacta aactattgga acctaagcta ttagaaatca aaggtttagg
     1741 aacgatcgct tttttaaaag aagaaagtcc ttctttttcg cagcttttac aacaataggt
     1801 atgttgataa tgggtgagac aatccaacat agcctgatgg aaacgaattt cttctcgtac
     1861 ttttgtccgt ccgatatctt tcatctgtga accgcaatgt tcacaaaatt gttcttccaa
     1921 aggaaggaga tgttctttct ctgttatag
//
LOCUS       DAMYXM010000162         1873 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.167, whole genome shotgun sequence.
ACCESSION   DAMYXM010000162 DAMYXM010000000
VERSION     DAMYXM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.167"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(365..1657)
                     /locus_tag="PNS21_002853"
     CDS             complement(365..1657)
                     /locus_tag="PNS21_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDI5160474.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          586 a          375 c          310 g          602 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
     1681 ttgtagaaaa agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg
     1741 gtttatatta cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt
     1801 atctatttga aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatccta
     1861 ttatccatat ctt
//
LOCUS       DAMYXM010000163         1838 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.168, whole genome shotgun sequence.
ACCESSION   DAMYXM010000163 DAMYXM010000000
VERSION     DAMYXM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.168"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            193..390
                     /locus_tag="PNS21_002854"
     CDS             193..390
                     /locus_tag="PNS21_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160475.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            496..978
                     /locus_tag="PNS21_002855"
     CDS             496..978
                     /locus_tag="PNS21_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160476.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1036..1632)
                     /locus_tag="PNS21_002856"
     CDS             complement(1036..1632)
                     /locus_tag="PNS21_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDI5160477.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT          569 a          276 c          368 g          625 t
ORIGIN      
        1 aggaatatgt tttaattttt tgaaaaaaat tcttctaaat acaacagctg ttttaattgt
       61 gaaaacgaaa acagtacgct ataatgcctg taaggttgat ttttaactgt aaataaaaat
      121 caatgagtta aatctccaaa gaaatagcag tggtaatgtg gagaaaagtt ccagcagttt
      181 aggaggattt tgatgacgga agaaatgatt aatttaggag aacaatatgc atgcaaacca
      241 attggtttta caaaaacggt cattggtgaa gttgtatcaa aaatgacaaa ctgcgccgtg
      301 gtcaaagttg cccaatgtgc tgcagaggac caagaattat tagatgaaaa agcaagcatg
      361 gttgttgcaa aatatgatac atttgaataa agaaaacaat tggacgtgcg gcgtgctgca
      421 agtccaattt ctttgagaag gtaaatcagt attcttttcc tattttctgg agaagaaaaa
      481 gcgaggcgag ttgtcatggt aacattcttt acggttctat tgtttttatt cgttgttgtg
      541 atgtttttat ttctttggct ggttcgaaaa gaaattattt atcggactgt gcgaaatcga
      601 tgggtctact tggtgatacc cttcttagtg tcactagtca tttggtatac actcatcagt
      661 catcctacag cagatgaatt ggcaaaaggg atcttgtcag caatgatttt tattagtttt
      721 ttgttggaca gtagaggaat cacagaagaa ggacttgttt tgaatagctt tgacaaaaaa
      781 ggtgttcctt tttctgagat caataaaatt gtattatatc agccaaaagg ttctaaaatc
      841 gtaaaaatga attttttccg aaacggatgg cgaggaccga tgctgaaatt ttctgcatca
      901 ttagaagaat tagttccatt tttgtcccaa cgcttaaacg atgaggcaga gattgatatt
      961 atgattgatc cagaataaaa acagaggttg cgacaagatg tttgtcacaa cctctgtttt
     1021 ttatttcaaa taaaattatc caaatacgcg gaagcgatcg gcatcgttca tgtattcttt
     1081 ttccccagcc ggtgtttctg gtgaaccgaa tactaattgc gaacgtagtt tccagtttga
     1141 agggatattc cattcttttg caactgcttc gtcaataact gggttgtaat gttgcaagtt
     1201 tgcgcccaat cctttatcaa ctaatgctac ccaagtatta gctgttgcga ttccgtttga
     1261 ttgttctgac caatctggga agttgtctgc atataattca aattgttctt gtagaccttt
     1321 taccacgtca gtatctttga agaataatac tgtaccataa ccatttttga aaccagctaa
     1381 tttgttttgt gtatttggga aagcttctgc tggagtcaat gggcgaagtg cttcttcagt
     1441 gatttcccaa agtttttcat gtgcatctcc gaaaaggata actgcgcgtg ttgattgagc
     1501 attgaaagca gttgggcttt ctttaattgc ttctttgatc aaagttgtta attcttcatt
     1561 agaaagtgaa acattacgtc ctagatgata gattgaacgg cggtttttca aagtgtcagt
     1621 aaaagttgtc ataataaaat ttctcctttg atttgcgttt tgcatttttt taattgatta
     1681 cgaaatgaag tatagcacct tacaaaaagt aagtaaacag atatgctcac aaaaaataag
     1741 cgaagttcaa aaaacaattt taataagaaa aatcgttatg taaggatttt cttagttatc
     1801 gctttcattg ttgtgtttta ggaatttttg cttatttg
//
LOCUS       DAMYXM010000164         1810 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.169, whole genome shotgun sequence.
ACCESSION   DAMYXM010000164 DAMYXM010000000
VERSION     DAMYXM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.169"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..528
                     /locus_tag="PNS21_002857"
     CDS             <1..528
                     /locus_tag="PNS21_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160478.1"
                     /translation="ENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIF
                     AKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPIST
                     ISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLP
                     ENWAELRKLVEYGII"
     gene            515..>1810
                     /locus_tag="PNS21_002858"
     CDS             515..>1810
                     /locus_tag="PNS21_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDI5160479.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFS"
BASE COUNT          598 a          233 c          281 g          698 t
ORIGIN      
        1 gaaaacagta aattgatgaa aaacatacaa tcaatctttg ggatagaaga agtttttgtc
       61 ggagataaat gtatcttttt tgatcaacct ttattatctg atggttatgg catagatgaa
      121 acagatattt tcgctaaagt aaatagtata ttttctggaa aaaaagaaac tttaattaag
      181 cttcatccta gagcaaggac aaatcgttat ggtgaaaaaa acatattgaa ttccagttta
      241 ccgtttgaag tcttgctact tgaaaatgac ttctcttcaa cacttttgat tagtcctata
      301 tctacaattt ctttttcacc atctatgatg tttgaaaaga cgatcaactc tattatgttg
      361 gctaaattaa ttttaaatga atatacagca ttatctgaag ataaaaaaga aacattgaca
      421 aatattgttc ttttctgtga acattataac cgtctatcag aagaaaaaat tttactccca
      481 gaaaactggg cagaattaag aaagttggta gagtatggga ttatttaaca cactttccaa
      541 gtcaaaatta gttaagtcca gtttctggta tactataggg aattttttta ttaagggaat
      601 ttcattcata actattccta tttttgtaaa tattactact caagcagaat atggtttgat
      661 taataatttc acttcatttg tatcaatttt ttcgattctt gttgggctaa gcctaaattc
      721 ggcaattaat aatgcaagtt ttgactataa agatgacata aatgaattta tgtcatcggt
      781 actttctcta gctacacttt cgttaattgt gttcttatta atagggaaca tatatttttt
      841 gttttcaaat gctttttttg atatgaacca acttgtattt aatttgatgt tattacaaag
      901 ttttgctagt tttgtaatta gttatatcca gtcttatttt acgattaact ttaaccattt
      961 taaatatttg attttatctt ttatatctac ttttttgaat attgcaatct ctgtagcttt
     1021 gatgcagaca ttatattttg atgataaata tgttggcaga gtagtcggcg gaacactgtc
     1081 atttgtgttg ataggtttag taatttattt aagaataatg ataaagggtg ctcatttttt
     1141 tgataaaaaa cagtggagat acgctttaaa aatctctttg ccgctaatcc cacattcctt
     1201 agcaaatata gttttagcac aattcgatag aattatgatt aattcttatt taggtcctgc
     1261 tgcgggggga atatattcct acattagtaa cataggtatt attctaagcg taatatggat
     1321 gtctactaat aacgcctggg ttccatggtt ctatggtgag atgagtaaaa ataatgaaaa
     1381 aaaagtgaag aaagttagta actattattt agtagtcttt actttgatta cgatgattgt
     1441 aatggtagtt tcgattgatt tagcaaaatt tatggctccg aaagactatt attctgggtt
     1501 atctttagtg gttccgataa ccttaggcta tttctttcag tttttatata gtttaccagt
     1561 taatgctgaa ttttatgaga aaaaaacatc gtttattgcg attggtacag tagcaagtgc
     1621 tatagttaat atactattaa acatgatttt catacctagg ttaggaataa ttgctgctgg
     1681 atacacaacc gtatttacgt attttttact ttttattttt cattataata tcgctaaaaa
     1741 aattacgaat aaacaattat ttgacacaaa aaaaatatgt atcataataa tttttgtatc
     1801 aattttttct
//
LOCUS       DAMYXM010000165         1783 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.170, whole genome shotgun sequence.
ACCESSION   DAMYXM010000165 DAMYXM010000000
VERSION     DAMYXM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.170"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            278..820
                     /locus_tag="PNS21_002859"
     CDS             278..820
                     /locus_tag="PNS21_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160480.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..1425
                     /locus_tag="PNS21_002860"
     CDS             976..1425
                     /locus_tag="PNS21_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDI5160481.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMAPIALDNEHPRI"
     gene            complement(1455..>1783)
                     /locus_tag="PNS21_002861"
     CDS             complement(1455..>1783)
                     /locus_tag="PNS21_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI5160482.1"
                     /translation="GKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVAESTYKSVKVE
                     FVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSD
                     TSGEAA"
BASE COUNT          657 a          264 c          336 g          526 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc ttgatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccagatggaa tggcaacact ttttttgaca aaaattataa ggtgttgtga atgctgcggt
     1381 gttatggctc cgattgccct tgacaatgag catcctcgga tctgattttc tccggcagaa
     1441 gcacaatcat tcaattacgc tgcctctcca gaggtatcag atccgcgctc attatcaagg
     1501 attcgcttcg ccaatctctg ttgtcgaatt tggatcggtg tttcatacgc caacgtgcca
     1561 tgtaagcgta gatagttcca ccaatgcaca taatcaaaca attctagacg tagctgtgcc
     1621 agtgtctcaa attggtattg atgcacgaat tctactttga cagatttata ggttgattcc
     1681 gcaacggcat tatcatacgg acaacccttc ctactcaatg aacgagtaat tccaaaacca
     1741 ttcagaatct catgaatggt ttggttatca aattcctttc ccc
//
LOCUS       DAMYXM010000166         1741 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.171, whole genome shotgun sequence.
ACCESSION   DAMYXM010000166 DAMYXM010000000
VERSION     DAMYXM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.171"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            84..506
                     /locus_tag="PNS21_002862"
     CDS             84..506
                     /locus_tag="PNS21_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160483.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            516..719
                     /locus_tag="PNS21_002863"
     CDS             516..719
                     /locus_tag="PNS21_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160484.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            930..1514
                     /locus_tag="PNS21_002864"
     CDS             930..1514
                     /locus_tag="PNS21_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI5160485.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          613 a          245 c          314 g          569 t
ORIGIN      
        1 aattttctag tatataactt gatttatctg ttaagattta ttaagaaaac gttttcttat
       61 ttttgatttc gaggtgatag acaatgaaaa ataagtttat tttatcttta ggtattttaa
      121 ctattggaat tagtgtaact ggaactacaa ttcctacatt tgctgatagt acaaatgaac
      181 caactactgt agtagataat aaccaagaat taactccttt agataggaac ggaatagcca
      241 ttggtaaccc aactaacgac ttaactgtta ctgaaaatta tttaatagtt tctccattac
      301 ttgctcgagc agcttctaaa acgatacgag tgacaggaac gatgattttt gataaagttg
      361 gttggccttc tagacctcct caatcacgga attggaaaga aaaaagaaat ggttattggt
      421 actatggaaa attgtattta caaagttata aatcagctgg aaacaattgg attgcgactt
      481 attcaggaac cttaactaga aagtagggat tttttatgcc agaatttgtg aacacaccaa
      541 atttttatca agatagcgat attctaagta aaattaattt accgaaagaa gaatcagctt
      601 tacaagttca gcgaacttta caggagacac aaaaaattca aaaatcaatt tctaatctag
      661 aagattctga agaaaaaaga aaaaaaagag accaaaaaac atatcaaaaa gaaaaataat
      721 gattttaaaa aaagagtgtc tagtcgacat tctttttttc taattgaaag tgttcataaa
      781 atcacccatt tctggacact tccttttttt gctttatttt tcaaaaaaac aattcatttc
      841 atgttcataa acccataaca atatctatgt tcagttaaac tatatggata aagttatgtt
      901 acaataaaat taatgaagga gtgatttttt tgaaaatagg atacgcccgg gtatctactg
      961 gtttacaaaa tttggattta caagaagaca gcctaaataa atttggttgt gaaaaaattt
     1021 ttactgatca tatgagtggg gcaaaaagta atcgaccagg attagaaatg gccattgatt
     1081 ttgttcgctc tggcgataca cttgtcgttt ggcgcttaga tcgtttaggt agaaatatgg
     1141 aagatttgat ttcaatcgtc aatcgactga atgagcgtgg tgtgagtttt catagtattc
     1201 aagaaaatat tacgatggac aaatcaagtt ctacaggaca gctcatgttt catttgtttg
     1261 cggcatttgc agaatttgag cgtaatttga tcctggaacg atccgcagca ggacgagcag
     1321 ctgctcgtgc aagaggacgg tttggtggtc gtcctgaaaa attgtctgaa caagatttgg
     1381 aattactgaa gactttagtg gatagcggta cacctattaa gactattgcg gaacggtgga
     1441 acgtctcaag aaccaccatt tatcgttatt tggcaaaaat ggaggagaaa aatggaaaga
     1501 caaattctca ttagtttttg tcatttaatg ctgaaaattt ttgccattta tctagaaaag
     1561 tgacaatagt atgagaattt tttgtaagtg tctactgata acttattatc aaatgttgta
     1621 aggattttat tcatttgtta atattaaaga acggaactcc aaaaaagtcc gtactctaat
     1681 ttgcagtctc tcactctttt agagtgagag tttttattag attttcaaat taattagtaa
     1741 a
//
LOCUS       DAMYXM010000167         1707 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.172, whole genome shotgun sequence.
ACCESSION   DAMYXM010000167 DAMYXM010000000
VERSION     DAMYXM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.172"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(151..441)
                     /locus_tag="PNS21_002865"
     CDS             complement(151..441)
                     /locus_tag="PNS21_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDI5160486.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(577..756)
                     /locus_tag="PNS21_002866"
     CDS             complement(577..756)
                     /locus_tag="PNS21_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160487.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(753..1103)
                     /locus_tag="PNS21_002867"
     CDS             complement(753..1103)
                     /locus_tag="PNS21_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160488.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(1096..1602)
                     /locus_tag="PNS21_002868"
                     /pseudo
     CDS             complement(1096..1602)
                     /locus_tag="PNS21_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
BASE COUNT          526 a          320 c          258 g          603 t
ORIGIN      
        1 tgcgagcagc aactagaata atttaaacat tattaaactg tctatattgt tactaataat
       61 tcccttctta tagtgcattt tcttagaaaa aaacaatatt acgatatcat gatattaaga
      121 tatcgtaata ttatacaatt gtgattgata ttatatatgt ttttttattt ttttatcatg
      181 tttaatcttg aaggaatcat attctttttc taaagacagg agcttttcat aaacatcaga
      241 catgtttaaa ctagaggcca ggcttaaatc ataaacgaat gaagcaggta atgattttat
      301 tctacgttgt cgggttatcc agcttgcaac cgttccttgt gggtaggcat ataactcaca
      361 aaattgttct acagtgattc ctaaattctt gataatgtaa acattgatcg ggtgcgagta
      421 aacaaatgta ttgatagaca tggtgcgtaa gcctactttc ttttattatt gcgtttgcaa
      481 taatagttta acacacgcat tattctagta tctactccta tttgtaaaaa caaaaaagga
      541 ttcatagaga taactctata gaatccgtaa aataccttat atatataacg ggaaaatata
      601 aaacctaaaa ttatgataac ctgctgcagc ttctacatag aaagagaagc gctcaacacc
      661 aacctcattt atcaaacgtt ggccataacc ccaaatctct tctgatagct gatcaaaggt
      721 tatatacttt agctcatatt gctggataat gttcattcta atcgactcca tttattatcc
      781 tggtaaaatt cgatatgacg tatttcgtcg tagtgaattt tgtcttttcc aacgtagatc
      841 cccagctcat cactacctaa gatcttgcca acaatatctt ctggatagta tccttcagcg
      901 tttcgttctt ctttttgaat agatactgat ctatttttta tgactgcagc gttcaatact
      961 tccgcaatct cttcagtcgt catttgttct tttgctaaat tttgactggt tcgttgttca
     1021 ttgtcggcat caatttcttc agtatgatca gataaataaa accctgccca ctttaaacgt
     1081 ccacgatcat tgtaatcaat catttttaat tcccccattt ccgcccgcat gaccaccaac
     1141 aagtgtgctt ctagtaatgc ttctagcgct ttctaacatt gaagaagcat gtacaatcga
     1201 cgtaaaacca tatttttgcc gaatcttgtc aataatttta tctaaatttt cttcgttaat
     1261 ttgcttttgt ggatcactaa ataaatccag ctgaagcgaa tcggtgtaca caagcttgct
     1321 ataggttatt ccaacgtgcc ttacttcttg tccttcataa tacttatcaa acaagaaaag
     1381 acaataattc tgcaattcct ttgtattatc tgtgataggt attttcatct ggtgtgaaaa
     1441 tcctggtctc gtttctaaga tcgacgtacc aatgtttaaa tgaacacatc ctgttttaca
     1501 attatgctgc ctgatacgaa tggctacttg ttccgccatt tcttttacca ccagctcgat
     1561 ttcatttctt ctagtataat ctcgtggtaa cacttgtgaa ttacggttct gttgcaaagt
     1621 tttaaatcta ctatcagata aggtagaata atagaaaaag atagcaggag gaatgacgat
     1681 gagtcatttt aaaggaaagc aatttca
//
LOCUS       DAMYXM010000168         1702 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.173, whole genome shotgun sequence.
ACCESSION   DAMYXM010000168 DAMYXM010000000
VERSION     DAMYXM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.173"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            51..1079
                     /locus_tag="PNS21_002869"
     CDS             51..1079
                     /locus_tag="PNS21_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDI5160489.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            1135..1353
                     /locus_tag="PNS21_002870"
     CDS             1135..1353
                     /locus_tag="PNS21_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160490.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1424..>1702
                     /locus_tag="PNS21_002871"
     CDS             1424..>1702
                     /locus_tag="PNS21_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160491.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNI"
BASE COUNT          643 a          195 c          319 g          545 t
ORIGIN      
        1 tttggattca aatgatgaaa tattatattt aaataaggag tatgataata atgaatagca
       61 aaaaacttca gatgggtgaa attgttagag gtcctagtca aatatctatt gatgttacat
      121 ataaatgtaa ttttagatgt aaacattgtt acaatgcgag tggtggagac aattgtttta
      181 tggataatga gttgactgat gatgaactgt tagaacttac aaaagatata tgtaatttaa
      241 agccatataa tatttgtttc tgtggaggag aaactttact gagaaaaaaa atagttttat
      301 cttgtgccac tttattaaaa gaggcaggta ttcctaatat tgctattgtt actaatggat
      361 cattagtaac tcaggatgtt gcaaaagaat tattcaaagc aggtattaat cgatttcaag
      421 taagtttaga tggaaaaaat gctagtagcc atgaagcact tagattatat ccaaacagtt
      481 ttgaaaaggc aattaatgct atcaaattat ttagagaatt gtccgaagaa gtggatgttg
      541 ctttttgtcc tactagtttt aattacaatc aattacgaga agtatatcaa ctagtaaata
      601 ctcttaaagt gaagtcttta agaattcaac catttatgct tagtggtagg ggaatgcaaa
      661 atagggaaat agtgccaact gtttctcaat atagaaaact agtttctgat attaactata
      721 ttaaaagaaa tgaagattat accaagatag attggggaga tccaattgat catatcatcc
      781 gacacagaac tttattgaaa gatttttcga attttgtttc tattaggtca gacggaggat
      841 taatggcatc accttacttg ccaattcaag ttggaaatat taaatgtcac agtctttcta
      901 aatattggcg agcaggactt tataaagttt tttctttaga tttagttcaa gatatagcta
      961 ctcaagtttc ttcaatacaa gatatgagta aagaattaaa aaaaggacat attaaatggc
     1021 ttaatgaaga tatagaaatt gatattctcg atgaatacaa acatgatatt ttaatttagg
     1081 tttatcattg gaaagtttag ataaacaaag tacttttaga aaggagggaa aaaaatgatt
     1141 tctgaacctc aaatgaataa tatgtatgaa agcccaacta taatgggagc tggtgaagct
     1201 gcgccaagag cttgttcagc aattatggtg tgtgtgttat tagcatctgc aggagtaggt
     1261 atccaagcag gagcggtgta tacttatgct gtagttgcta cagtagctgt agctgcagcc
     1321 gcagtttgga aaacagtagg attagttggt taggaattaa taggagacta actgtctcct
     1381 attgattcca atgattaagc taaggaggaa ttttaagaaa ataatgaaat acaataattt
     1441 aaaaccgaaa ttacttaatg agaacataga atatataaga gaagaaaatt atggatattt
     1501 aaccatagtt cccaaaaatg gaactgaatt gaaagataac gttttcaatc caacagtaag
     1561 atatatattg gctaactgta ctggaaaaaa cagtgtaaaa aaaatagcga atggtttaaa
     1621 aaagaagttt cctagtttag aatatgaaaa attctatcaa gatgttgtag gaactattaa
     1681 atttttcaaa cactataata tt
//
LOCUS       DAMYXM010000169         1664 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.174, whole genome shotgun sequence.
ACCESSION   DAMYXM010000169 DAMYXM010000000
VERSION     DAMYXM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.174"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            131..505
                     /locus_tag="PNS21_002872"
     CDS             131..505
                     /locus_tag="PNS21_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDI5160492.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="PNS21_002873"
     CDS             complement(619..1248)
                     /locus_tag="PNS21_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDI5160493.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="PNS21_002874"
     tRNA            complement(1382..1454)
                     /locus_tag="PNS21_002874"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAMYXM010000170         1621 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.175, whole genome shotgun sequence.
ACCESSION   DAMYXM010000170 DAMYXM010000000
VERSION     DAMYXM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.175"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            289..696
                     /locus_tag="PNS21_002875"
     CDS             289..696
                     /locus_tag="PNS21_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160494.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            710..1111
                     /locus_tag="PNS21_002876"
     CDS             710..1111
                     /locus_tag="PNS21_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160495.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1113..1484
                     /locus_tag="PNS21_002877"
     CDS             1113..1484
                     /locus_tag="PNS21_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160496.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          606 a          208 c          282 g          525 t
ORIGIN      
        1 tttgagggga agtacatata gttgttgata tagttgctaa tattgttagc taatttgtta
       61 gtaaaactgt atacgaattt agatagtaaa aaaacagttt gttccttata tagaggcact
      121 tttactactg attaaatgtc agatatgctt acaaatatac ttataaatat gcttacaaaa
      181 ttagctaata tttccgttga caaagctttg ttactattgt taaactaaat tgactagcaa
      241 aagtgaaaca taaatttatt aaataaatgt aaaaattagg agaaatcaat gtcttataga
      301 gtacaattaa taatttcaga agatgtagaa agtcagcaat ttggcactaa tgttattaat
      361 aaagtaatta atccagctct ttctattaat gcgcctttaa ttcctactgc tttatcattt
      421 tctgtaacag ctgtggtgtc tgagatagag gatacagaaa agataaaaat agtagaaatt
      481 gaagttttaa acaagaatga aaaacaaatt ttttctacgg gtgaagtatc ggtaagcttg
      541 cctcctcaag ttaacgatat aaactttaat ataaacgcca gaaatgtctt ggttgaagaa
      601 gcaggagagc attatgctgt tgttaaattt aatggaactg agattggaag gcaaatcttt
      661 gatatcaagg tcaacaaacc agtggaaaaa aattaaggag acgatacgga tgcttgatat
      721 agtaccatca aatacacatc ggagtggtag cttaataaat tttgctgcga tttttgcttg
      781 cgcatcggta attctagcat ctccagttgt tgtggatgca agttctactc cgaaaactaa
      841 aaatgataat caacttgtaa ttactacaaa aaatgagatt aacacaattt caagtaatga
      901 tggcaaaatt tttgatgtga taaatacggt tttaaaaaga aaagctgaaa tcaatataaa
      961 atatgatgaa gatttaaact tgtatttctt tgttatcaaa acaacatctg aattgttttc
     1021 ctcagattac gatgttttgg atactctcga taatgtatta tctgactata aatatatggg
     1081 gaaatctgta gtagcaactt tgggggaata aaatgtttga ctttaatgaa tatttatcta
     1141 tttccaaaca aattgaatca gatgataaat ataattctaa agaatcttat agaagaactg
     1201 ctgtttcaag agcatactac agtgctttta aaaaatctga tgagtatttg aaagaaaatt
     1261 atgatattat ttataatgga agtagcggaa aaggaagcca ccaaaccgtt tggaatttgt
     1321 tttcaacagt taaggaattg aataccttag gaatacataa tagtgggtat agaatgttag
     1381 agaaaagaaa atgtgctgac tatattccta atgaaaccat tacgaaaaca gatatggcgc
     1441 tgatgaatcg agaagcagaa aaaataataa acaaactcac ctaatgaatt tcaagcgagt
     1501 ttgtttgtta tttttttatt taatctttgt atctttctgg atcaacgaaa gtatacttta
     1561 tatagtcata acgccgatga tcgctacgtg cgtccggcac gtcagtcacg atatcaaaca
     1621 a
//
LOCUS       DAMYXM010000171         1597 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.176, whole genome shotgun sequence.
ACCESSION   DAMYXM010000171 DAMYXM010000000
VERSION     DAMYXM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.176"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            87..1553
                     /locus_tag="PNS21_002878"
     CDS             87..1553
                     /locus_tag="PNS21_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDI5160497.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          445 a          292 c          332 g          528 t
ORIGIN      
        1 atatttctta aataagtttt ttagttaaat ttttcggtaa agatgtataa tattttctga
       61 aaatttaaag aattggggta tagattatgg aaaaacagca agacaagggc ttcttcgggc
      121 agccgaaggg attgtcaact ttgttcttca ctgagatgtg ggaacgattt agttattacg
      181 gtatgcgtgc attacttatc tattatatgt acgatacagt tgccaacggg ggacttggat
      241 taccacgagc aacagcatta gctatcatgt cgatctacgg atctttgatc tacatgtcta
      301 gtatcgtcgg cggatgggta tctgaccgag tgttaggagc aaaacgcact gtattcttcg
      361 gtggagtttt cattatgctt ggccacatcg tcttggcttt accatttggt gtaagtacat
      421 tgtttgtttc gatggcattg attattatcg gaaccgcctt tttgaaacca aatgtttctg
      481 gcatggtcgg acatctttat tcaaagactg acttgcgaag agatgcagga ttttcgattt
      541 tttacatggg tgtcaactta ggggcattat tagcaccact aatcgttggt acaatcggtc
      601 aagaatataa ctatcattta gggttctctt tagctgctat tgggatgttc tttggattat
      661 tacaatacat catccaagga cgcaagacat tagacggagt gggtacaaca ccgccgaatc
      721 ctttgacaac tgaagaacgt aaaggattca tccgtaactt gatcatcgca cttgttgtta
      781 tcgctgcagt attcggcggg gcacaactaa ctggtcatct gacaattgac ttttttgtca
      841 atacaatcag tgtattgggg attatcttac cgttgtatta ctttatcaaa atgctgactt
      901 caaaagatgt aactgctgaa gaaaaaccaa gagtatgggc ttatatccca ttattccttg
      961 cagcaatcat tttctggtca ttacaagaac aaggttcatc tatcttagct ttatttgcta
     1021 gtgaacgtac acaatctacg ctatttggtg taacgatcca agcaagctgg tatcaatcat
     1081 tgaatccaat tttcatcttt attctgacac cagtattcgt aacgatgtgg acgaaacttg
     1141 gtgaacgtca accatctaca gtagtgaaat tctctcttgg acttttattt gcaggattat
     1201 cgtacttgct attgatgttg ccgggtatgc tttatggtac aagtggaaaa gtaagtccat
     1261 tgtggttgtt gggaagtttc ttcatcgtgg ttattgctga attgtgtctt tcaccagttg
     1321 gtttgtctgt gacaacaaaa ttagcaccac gagcatttga atcacaaaca atcgctattt
     1381 ggttcttagc tgatgcttct tcacaagcaa tcaatgcaca aattgcacgc ttctacacac
     1441 cgggaacaga atctgcttac ttcggtatta tcggtgcagt agcaatcgtc ggtggtatct
     1501 tgctatttac tgtgaaagaa ccaatcaaaa aactaatggg ctctattcat taatcaatga
     1561 acataaaaac aggaattgct tatttgcagc agttcct
//
LOCUS       DAMYXM010000172         1585 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.177, whole genome shotgun sequence.
ACCESSION   DAMYXM010000172 DAMYXM010000000
VERSION     DAMYXM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.177"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..361
                     /locus_tag="PNS21_002879"
     CDS             <1..361
                     /locus_tag="PNS21_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160498.1"
                     /translation="FRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCRTC
                     IQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQSII
                     GVKTLAYLSGMGRNRRF"
     gene            679..1269
                     /locus_tag="PNS21_002880"
     CDS             679..1269
                     /locus_tag="PNS21_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HDI5160499.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            1296..>1585
                     /locus_tag="PNS21_002881"
     CDS             1296..>1585
                     /locus_tag="PNS21_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDI5160500.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIR"
BASE COUNT          576 a          174 c          307 g          528 t
ORIGIN      
        1 ttttagagat aatgagttcg attgtaatat tgaattagtt aaaaaaattg aaggcgattt
       61 aagctatata ttagaaaatg agatatttgt aaacaaaaca aggagacttt tgttgggacg
      121 ctgtcgtact tgtattcaaa aggcagttag atatgctagt tttaataaag aatttgattt
      181 aaactcctat ttgtctaaga tacttatgaa cgatgagttg attgcctgta ttcactcata
      241 cccatataaa caaaatccaa ttaaacaacg aatatttaac tattttttac taaaccaatc
      301 cattattggg gttaaaacat tagcatattt aagcggaatg ggtagaaata ggagatttta
      361 atcataaaaa actgtactat agtgaatttt tggagattta tattccagtt atatattaat
      421 gattgtgata cttgtactat tgtaaaaatt cgttaggttt aaataactgg aaatatcgga
      481 aaatgggcat tatacatcac ggacatccct tcttttggaa aaaatgttac gatttttata
      541 agtaatattg tatccggtgg aatcaacgat gaagataaca tatccatcgt tgtagattca
      601 gtagcattga atgatgctct tgttgcaaga aatccgaata aaattgttaa gtataaattg
      661 aattaatagg agatatgaat ggaaattagc agtaaagaat atttaaaagc ggttcttcgt
      721 atggaaaaga atctatatat agaatctaaa ggacaacaca caaaattact aaaaataatg
      781 aaatcacatc cggactacaa cgcttggcgg tatactcgtc gaatgagaat tacatcttat
      841 tattattcaa agagaaagaa aaatatcatt tatgcgatta tgtatattgt gtcttgcaga
      901 agaatgaata gtttgggaag aaaatgtggt atagagtgtg gtgaaaatgt atttgatgaa
      961 ggtgtaagat tttaccatac acaaggaatt gttattaatg gtaacgccat aataggaaaa
     1021 aattgttact tatatgggaa taactgtatt ggaaatgatg gaatcgatcc taagtgtcct
     1081 gtaattggaa ataatgtacg tgtatgcgtg ggtgcaaaga ttattggtaa tgtaaaaata
     1141 gctaacaatg tagttgttgc ggcaggagct attgttatca aagattgtct agagaatggt
     1201 gcaattcttg caggtgtacc ggcaaaaata atagggtatg ttaatttaac aaaagtattt
     1261 ttagaataat ttttagcgaa agaaggtgaa taacgatgcg tatttcaata aaaagatggt
     1321 caggagcaga ttggacattg ttcacgttgg cattttttgc aatctccata gttcttgtat
     1381 atacttttaa cgccccaaga gcaattttat atttgggaga tataattaat atctttgtgt
     1441 tattaaatgc acttagcttt agacgcagaa aaggaatata tgctagcaaa aatgagatgc
     1501 tgatagtttt gtttttacta gtttctattt gtagctcagt tattaattta gaaaatatag
     1561 gattgattat ctgggggata cgaaa
//
LOCUS       DAMYXM010000173         1575 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.178, whole genome shotgun sequence.
ACCESSION   DAMYXM010000173 DAMYXM010000000
VERSION     DAMYXM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.178"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(856..1386)
                     /locus_tag="PNS21_002882"
     CDS             complement(856..1386)
                     /locus_tag="PNS21_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HDI5160501.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1383..>1575)
                     /locus_tag="PNS21_002883"
     CDS             complement(1383..>1575)
                     /locus_tag="PNS21_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDI5160502.1"
                     /translation="KIWSSIVYGGKIAFYFSAIIEFLQLFLRLGTFQISDFVYNTLGG
                     ILGVILYKLILITKRDGER"
BASE COUNT          491 a          315 c          179 g          590 t
ORIGIN      
        1 aatttaaaat taacattgat tctcctatga aaatatcttt tacctttctc ctgtgaaaag
       61 gaaatttcct tttttatagt gttattgata taatcagaat attcatcaaa ctctgtatct
      121 ggagttaaaa atctacagta acatctataa gctttgctag taatcttctt gggttctctc
      181 tccattgtca agcaacatca aaacacctac ttactattcg attcttttct gccaaaaacg
      241 agtcagcatc actcacaact tatgtgttgc tgactccgac tagtcctttg ttcaatatca
      301 acttattaaa cagatataat atttttcttt atttcgtata ctccaagact tccatctttc
      361 tttcatctta tctctgaaaa aaacacagca aagtgccctt aataaatata cattagaatt
      421 tgcaaatttt ttcatcttaa gatatatcct attcatattc tttacaatcc taatgggtga
      481 ttacaaatct atttagttca agttctacat tataaacacc tttcttgata gtatcatctg
      541 ctgcacaagc gatgtcgatc cacttattag gggatttacg tgctcaaaag tggctattcc
      601 ttttgggaag tccatccata ataatggtcc atggattttt ttagggtctt gtcgttcaaa
      661 tttctcaaaa actttattat acttccatag ttttataatt atttggtttc actttggctc
      721 gttcaaacac caacttgtat ttcttttcaa cgttcgaact ggagcccccc ctataatctc
      781 attattattt tcatgacttt ttactaccgt ggaattacaa gcaacaacaa tattatctgc
      841 taaatgaaca cctttctata tcacgctttt tgctccaatc caacaatgtt tacctataac
      901 tattttatta tttttattca aaatcctatg agtattcaat tcaaaaatcg tatgtgaatc
      961 ggtatccata aataatgtat cccacgatac tgtacagcca tctttgaatt gaatttcctt
     1021 aaagcaaatt atactcattt ttcctgtcga attaaacttt gacccaatat ctaaaactgc
     1081 atttttcctt atgtcaatat gagccccttg tgcgataaac gctggccctt gtattcttag
     1141 tattccttca atacttataa tcgtttcttc atttcccgca gtatttaata ctggaaagcc
     1201 caaataaact atttttccag attgtaatac tatctcaatt ttccctctcg atttgcgtgt
     1261 aatccgtact ttacgatgca ccaaaatagg taatctccac gcatctgaaa tatgaagaaa
     1321 tttataattg aagtatatcg ttttaggtaa atttaatata ttctttatta tcaatccgag
     1381 tctcatcttt caccatctct ttttgtaatc aaaattagtt tataaaggat tacacccaat
     1441 ataccgccca acgtattata tacaaaatcc gaaatttgaa acgttcctag cctcaaaaaa
     1501 agttgtaaaa attcaattat tgctgaaaaa taaaatgcaa tttttcctcc atatactatt
     1561 gaggaccata ttttg
//
LOCUS       DAMYXM010000174         1566 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.179, whole genome shotgun sequence.
ACCESSION   DAMYXM010000174 DAMYXM010000000
VERSION     DAMYXM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.179"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(69..224)
                     /locus_tag="PNS21_002884"
     CDS             complement(69..224)
                     /locus_tag="PNS21_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI5160503.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            841..1062
                     /locus_tag="PNS21_002885"
     CDS             841..1062
                     /locus_tag="PNS21_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160504.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1063..1320
                     /locus_tag="PNS21_002886"
     CDS             1063..1320
                     /locus_tag="PNS21_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160505.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKAAKSAVPTGFLTKQPYIF"
BASE COUNT          535 a          351 c          246 g          434 t
ORIGIN      
        1 atgctataat caccttaatt gaacaacagt ctaacagcaa aagcagtgcg ccaacactaa
       61 tgcttttgct attttttttg ctcattttcc tttccgttag cattaaccca aattgtaata
      121 agaccagact tactaagacc catttgtttc gcaattttat caagattatc tgccacttcc
      181 ttagtaagcg ttacaacgac tctctttttt tcattactga tcataattcg tatcctccaa
      241 cgactttttc ttaaaaacca tagtaacaaa aaagtaacta aggtgcaaca gtttagatac
      301 taattagcga ataataggcc ttaaaccctt ggcattactg attttgtcaa aaaaaaacgc
      361 tgaaaccctt gggagagtaa ggctaaacag ctatcgacca atgaccaatg accaataagg
      421 ggggtgtcgt agtccccccc tcctccgccc aattcataaa caccaaaacc aaaaaacaaa
      481 cctcagggct ccgccccgaa ccccgacctc cttctaccga aatatatgca ggcgccacgc
      541 ttactatttc gttcagctat cggcttttgc aatctaacac gaccgtttcg ctaccagtcg
      601 ccttacaggc tccattctcg cttctcttac gtgttttaac gattgcgccg ataactaaac
      661 gaaatagacg ctaaatcgct taaaacgcaa aatagaccct ttttagagca ttgatttttt
      721 ctactagtca tttcgataat ataaaattga ccacaacaca gaaaacttat attataagct
      781 tatgtcgtaa gtccgacgta ctcacgtaat atagacggac gttaaatgga aggagcataa
      841 atgagagtga taaaatcaga aaaattcact ttggccgaaa ttatgacgac cagcgaactt
      901 gcacaagaac ttgattacaa ccaagcttac gttttgcgct tagcgaaaga acatctgact
      961 gaaggaaaag aatatcgctc tgccggtaga agaaactatc ttttcagccc agaagccctt
     1021 aaaaaactag aaaaagtcat aaccaccaaa gaaggtgagt aaatgcctaa agaaacaact
     1081 atcaacgttg aacaactaga aaaattgcta gaatcatatt ccaatgattt tatcgaattg
     1141 accggcggaa aagtcgaaga aaccaccgac ctctttgacc tacttctcac aaatattctg
     1201 gaattaaact ctgaagaaca aaaagagcat aagggaaaac tgatagcacc ttcctacagg
     1261 aaggcggcta agtcggctgt gccaactggt tttcttacaa aacaacctta tattttttga
     1321 tatcacaaaa aaatccattt tggacctatt ttcggtcata atatagtacc tacttttggt
     1381 catagtttcg tccgtagttt tggtcatagt gactaagact ttcacgcaca aaaaaagagc
     1441 tgaaattaac agctcttatc ctttatttat ttttgaattt acacttccac catgacgatt
     1501 cgattttata tattgcccga taatataagc tgaattatca caaagaagcg gaaaccaatc
     1561 atcaaa
//
LOCUS       DAMYXM010000175         1539 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.180, whole genome shotgun sequence.
ACCESSION   DAMYXM010000175 DAMYXM010000000
VERSION     DAMYXM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.180"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(110..1150)
                     /locus_tag="PNS21_002887"
     CDS             complement(110..1150)
                     /locus_tag="PNS21_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDI5160506.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
BASE COUNT          492 a          236 c          202 g          609 t
ORIGIN      
        1 ctattaaaat agctatttct ttgtaacatt tgtaatataa ttagaataat aaaaaatttt
       61 taattttaat gctactccat gaagcaacaa tcaatttgtc agtgaaattt cactctaacc
      121 agtctttcgt aatagttttt gggaagcttt cagaattcaa acgtttttca cttgatactt
      181 tagcagctat tggtttttcg ataaaccaat tttttacata gacaaaaatc aggttctgga
      241 tattttcaac tttttcggtt tttaattttt gataggcttt taacagtgtc gtgtataatt
      301 cttgttctgc attgacataa taactatcga gttcttcaaa aattattgaa ataccacttt
      361 ctgtttcggc ttttttcttt gcattatgaa tagtctggac agttttttga gcttcactgt
      421 aacttgaaga aaatgttttt attaaattta atactcgttc agggataaac gtagcaatgc
      481 tgtcgtttgt cataattgtc tcaaaattgt ctaacaacaa ttgatcttgt aggcggtctt
      541 tttcagtgtc tatattgtgt ctattagtgt ctaatatatt attattaagt tctggtatga
      601 ttttcatacc ctctgattta tcaacgtttt ggcacggtat gattttcata ccctcttgag
      661 ccccaaggga ttcagcgtct gatttttcgt cgagggtggg tatgattttc ataccctctg
      721 atttatcaac gttttcccta ttctctctat caaatttttc gagtaagtag atatcatttt
      781 ctgagacttt taattctgct agatatagtc tattaggatt atttttccct aactgtttgt
      841 taaatcccat ctgcttttgt aataataaac caaaggattc caactctttt ttgattgcta
      901 tcgctttttg tttttggcag tttaatacgc tacatagttc atttgtcgta taagtaaaat
      961 aaacattacc ttcttcatcg atttgccgtt tgctgattgc gatctctaat cttgctttta
     1021 aaagcatata ggcaatctta gttgaatctt tcattttttt gtattcgtca gaaacaataa
     1081 agacttttgg aaattgataa aataattcgt tgtaaactct atccgcctta aaaaaattaa
     1141 aatcagtcat tgtagatcct ctactttcac gttgttttat ttatgtaaaa ttacctactc
     1201 ttttcgaaaa tagcaaagat aaataaaaaa tgcaaacgca ttaaaactgt tttttaatta
     1261 aatactacct aaaatcagaa ataaaatgaa ttatgtacta cctttaaagt cattaagaac
     1321 tactttaaat aaatattatt tataattaaa tactaccaga agaacgtatg aataaaattt
     1381 agttttaaaa ttaaagataa ttaagtacta ccagtgtcaa aacaaaaaat aaattaagaa
     1441 ctaccttatg catataaaac atactaccat tactagtaat atatactacc ttatttcagt
     1501 agtatatatt atattcttct tataagacaa ggctttagg
//
LOCUS       DAMYXM010000176         1511 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.181, whole genome shotgun sequence.
ACCESSION   DAMYXM010000176 DAMYXM010000000
VERSION     DAMYXM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.181"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(287..360)
                     /locus_tag="PNS21_002888"
     tRNA            complement(287..360)
                     /locus_tag="PNS21_002888"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(324..326),aa:Ile,seq:cat)
     gene            complement(549..1508)
                     /locus_tag="PNS21_002889"
     CDS             complement(549..1508)
                     /locus_tag="PNS21_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDI5160507.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          447 a          298 c          273 g          493 t
ORIGIN      
        1 ataaaagagt ttaaagcgta acacacttat ggggaagtgg cttggggtgc gctttaaact
       61 cttctttatt atcatctcac aatttaaccc aaatgtcttt ctatttaaaa atcaaagtaa
      121 aacttttcaa atatacagaa atataactat gtgaaacatc ctttcattaa tccataaaag
      181 gatacataaa aaagccactc atttgagtgg caatgaagaa aagctttagc ttgtataata
      241 ctcttcaaaa aattctaaca cagaacgatt caaatggcta cgttaatgta ccctgtagga
      301 ctcgaaccta cgaccggacg gttatgagcc gtctgctctg accaactgag ctaagggtac
      361 tggttgttgc cacataaagc cataaacaat caaccagtag aatgtgtggc aacaaacctg
      421 ttatcgcata gcttggagtg tgactattat gggtgatagt gaagatatgc gataacatca
      481 ctattttatc gaatgatttt tatagttgtc aatatagtta tgtaatgctc ctcaacgagg
      541 agctattttt atcgtttagg aatatttaaa taccaacgtt tgtcatggaa atcttgcgca
      601 ccgcctttag tgtttccctc tggatcattc gttgcccgca tcatgacata gactttctta
      661 ttagggaagt tacgcatgtt aaaagataca tgatagccaa catttccaga agtattataa
      721 gcttgattta catctgatct ataaattcca tcagctctta ctcgagctaa ttctttccca
      781 gtattgtaat ccataatgaa gatatactcg tatttatagt tagcaatgtg ccatccagct
      841 acatgcaaat ttgcattttc gatttctcca aactgatcaa tgtgggcgta attcgttcca
      901 tctgtcagtg taggattagc tgcaccagct cgtgttggat caatgacagg cttgttttca
      961 gaagttgttg gattttcatc ggtaaatcca tgagctaaat catatgctaa tttttcttta
     1021 cttacgccca tttcagaaag ataaccgtaa ggatctgtat gatcgcccca gatgttttgt
     1081 gttacccata aatgcgattt gattcccggt tggttataag gagtgtccaa cgttaatgga
     1141 ataccatatt tcattgctga atctctagcc aattcaacgt atgccttgta gtttttctca
     1201 aacgttgctt tatcatgtgt gtgttgtaac tcaatctgca caggactgtt ggcattagca
     1261 tacgaaccag caccgtactg tacataacca ggttgaccga cttgataaac aattccgccg
     1321 tctcccacaa tataagcagt ataagcgcta gtccatgaac gttgcatata ctgcgcttca
     1381 ttgcgtcctg ttgctgtttc attagccgtt tcatgcagta aaatatactg attatttgct
     1441 acttgtgagc taccttcatt tgcgcccaaa ttaaattcat tgttgatagt ataggcaaat
     1501 ccgttaattg g
//
LOCUS       DAMYXM010000177         1480 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.183, whole genome shotgun sequence.
ACCESSION   DAMYXM010000177 DAMYXM010000000
VERSION     DAMYXM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.183"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..331)
                     /locus_tag="PNS21_002890"
     CDS             complement(<1..331)
                     /locus_tag="PNS21_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160508.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYK"
     gene            complement(333..1391)
                     /locus_tag="PNS21_002891"
     CDS             complement(333..1391)
                     /locus_tag="PNS21_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDI5160509.1"
                     /translation="MVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPGLLARQGF
                     IEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSS
                     SRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAK
                     DFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVI
                     RPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAF
                     ICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISI
                     VFWYYLGSISYLYLKKEN"
BASE COUNT          577 a          241 c          179 g          483 t
ORIGIN      
        1 ttttgtaaac attggagaaa agtatttttg tgaaataatt ttccccctgt ataaaaattt
       61 catcataaac ggataaatca tccgagaaaa attgattatg gttagttaac aagttaattt
      121 tacgtttaaa ggaagaaccg attttccggt gattcacaaa aacaacatca ttaaatagcc
      181 ctaattcttt tatacgctca ctatatcttc tagcgttttc aaattcagaa taaataaata
      241 aatctccttt tgattctcgg taaaaatttt gataaacatt taatatatta atcaaatgta
      301 aaggagtcca agcaatgaaa gctgtcttca tattaattct ccttttttaa atacaagtaa
      361 gaaatgcttc ccaaataata ccagaaaaca attgaaatga catcgcgctt attgaaaagt
      421 acatgagcct ccaccatttc atctataaaa atagatagta taaaaattaa aacaatacca
      481 agaaacgcat aattcacttt acttctaaaa aatgttttga gtaaactaaa gatatttaac
      541 acatagaaag catagataca tataaaagca attataccac ttgatacaag tacttgaaga
      601 aatccattat gcatgtttcc atgtgctctt tttagttcat tcacatctgt agtatcaaga
      661 tctacttttt tcaaaaattt tgcatcgcta ttcccataga aatcggtctc tccataacca
      721 aataacaaag ttggtcttat aactttaagc gcagctttcc aaatcgtaaa acgaccagct
      781 gaaatttcgg agttatcact agaatgctgt aatattactt ctttttctgg aagtggagtt
      841 ttatctgatt catttgactc tataacttct acttttctat caatcgatac aggaattgtt
      901 gctaaaaaat ccttagcata attagtacca aaagaaaaat ataccaatag aactaataat
      961 atgcttacca tccatttgat ttttacaggt agtacttctc taatgctctt tctaaatttt
     1021 gaattggcaa acaacaaaag aaataatact aaaaaagaac aaattactac ctgggcacct
     1081 ctagacgaag aaagtgtaat ataaaatatt tgtacgataa aattcatcat ataaaaaaca
     1141 cgaatcgttt ttgttatttt tgaaattaat acactaatta aacttaaaat aatagaagta
     1201 tatccaaaca tagctccaac atttggactt gtatagacac caaataaccg atgttcaata
     1261 aaaccttgtc ttgcaagtaa tcctggcgtt cctgttggga catgataaga aatcaaaaaa
     1321 ataaattgat agatagatat tagcgcagct aaaaaaatta tgataatgaa aacattatta
     1381 aaatacacca tttttttctt tttttcctct tcactttgtg aaaagtctgc aggaaaaatt
     1441 actatcaaag taatcagcaa atatccaaaa ttataaatag
//
LOCUS       DAMYXM010000178         1453 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.184, whole genome shotgun sequence.
ACCESSION   DAMYXM010000178 DAMYXM010000000
VERSION     DAMYXM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.184"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            108..1040
                     /locus_tag="PNS21_002892"
     CDS             108..1040
                     /locus_tag="PNS21_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HDI5160510.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1453
                     /locus_tag="PNS21_002893"
     CDS             1112..>1453
                     /locus_tag="PNS21_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160511.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQ"
BASE COUNT          639 a          169 c          226 g          419 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caa
//
LOCUS       DAMYXM010000179         1453 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.185, whole genome shotgun sequence.
ACCESSION   DAMYXM010000179 DAMYXM010000000
VERSION     DAMYXM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.185"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            60..467
                     /locus_tag="PNS21_002894"
     CDS             60..467
                     /locus_tag="PNS21_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160512.1"
                     /translation="MFFLPLKYLEIYSVKKNKLIGENDSPIKTPIELSLRNRMGILQR
                     SEIVKELFYHKFSYEIRLDDTYEHYRIVFFVYNSIEDATATTALTFGFTKNGTINSDK
                     TRQAATESDDICKKVCNGEENCWIGEEKLNEIY"
     gene            454..906
                     /locus_tag="PNS21_002895"
     CDS             454..906
                     /locus_tag="PNS21_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160513.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            899..1297
                     /locus_tag="PNS21_002896"
     CDS             899..1297
                     /locus_tag="PNS21_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160514.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
BASE COUNT          565 a          182 c          247 g          459 t
ORIGIN      
        1 ccacacctta taaaaatctt tgacaaatct attcgaagga tcgaatttaa accataattg
       61 tgtttttcct tccattaaaa tatcttgaaa tttactctgt taaaaaaaat aaattaattg
      121 gtgaaaatga ttctccaata aaaacaccta ttgagctaag cttaagaaat cggatgggaa
      181 tactccaaag atcggaaata gtaaaagaac ttttttatca taaatttagt tatgaaataa
      241 gactagatga tacttatgag cattatagaa ttgttttttt cgtatacaat tctatagaag
      301 atgctactgc gactactgca ttaacattcg gttttacgaa aaatggaaca atcaattcgg
      361 ataaaacccg tcaagctgct acagaaagtg atgatatatg taagaaagta tgcaatgggg
      421 aagagaattg ctggataggg gaggaaaagt tgaatgaaat ctattaatga tttattaaaa
      481 aaacatcctg atttatataa aaaattaaat tctgaacaaa gaaacctgtc caaacaatta
      541 cttaaaataa gattaaaaaa acaaaaaaac gtgacagaat tctcaaaaat gttaggaata
      601 agtgaaaaaa aatatcttga atttgagttc ggagatatgc atattcctgt tgcagagtac
      661 aataaacttt tgaaacaagt tgaaaatttg tcctataaag attcaatgaa tttcactatt
      721 aaggttgctg gcggccctat taaatatgac cgtattgaaa ataaacctta tattcggaat
      781 aaaagttact cagtatttaa aagtagaaag agaaaagaag tatcgtcaaa tatggtctat
      841 gctagtgtta attaccaaga tacgagccgc tatattgtag aaaaagaggt atatgtacat
      901 ggataaagga aatagtgtta cttttgcact tgaaaatatt agtttgaata aaattcattt
      961 tgaattaact gataatttta gttatggaga tttagagttt aatattgaac ttggatataa
     1021 agaagacgaa gcagagtatc tagaaaaaaa acttgtttct ttacgagctc acattgtagg
     1081 ggggatggaa ggagcaccgg tagatatttt actcttttca gttattcgtg tttctacaaa
     1141 tgaacagcca tataaaaaat tagtagaaga atctatggga gagctagcta aacctatcct
     1201 gaataaagct tctaatttga tttctaattt atatagcgaa gtaggagtta tgcctacagt
     1261 tattgattta tatcagctat atagtcaaca aaaataaaat acaagtaata gagttatcta
     1321 tttaaatttg caagacaaag acctcaacca gtcatccttg gttgaggctt ttttgctctg
     1381 taattaactc gtatagatcc tgcaagatag tttgtttctg aataaatgat tgatatgaag
     1441 tatttagatg act
//
LOCUS       DAMYXM010000180         1451 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.186, whole genome shotgun sequence.
ACCESSION   DAMYXM010000180 DAMYXM010000000
VERSION     DAMYXM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.186"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            14..424
                     /gene="spx"
                     /locus_tag="PNS21_002897"
     CDS             14..424
                     /gene="spx"
                     /locus_tag="PNS21_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDI5160515.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            438..794
                     /locus_tag="PNS21_002898"
     CDS             438..794
                     /locus_tag="PNS21_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HDI5160516.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          535 a          218 c          270 g          428 t
ORIGIN      
        1 aaggagtgag aaaatgataa aagtatatac ttcgcctagc tgtacttcct gtagaaaagc
       61 aagagcatgg ttgcaagcga atcaacttga atttgaagag aagaatattt ttgccgaacc
      121 gttaacagaa agtgagatca aaaagatctt ggcagcgact gaaggaggag ttcaagatat
      181 tatttccact cggtcgaaaa tttacgaaaa gctgggcata gattttaacg aattaacttt
      241 aaagcaaatg atcgtcttat tcaaagaata tccttcatta ttgagacgac ctattttgat
      301 tgatgaaaaa agaatactaa tcggatttaa tgaagatgag atccggtcat ttgtccctcg
      361 tgaaattcga tgtgttgcca gaaatctgat ctatttacaa aacaataaca tcttaaattc
      421 atagggaggg agccttcgtg gaagtgaaag tctatggatc agcgcactgt gtaacaagtc
      481 tgaaggcgaa acaatggtta gaacaccatc aattgcctta tcaatttgtt gacttagaag
      541 aacgcgatct aagtacagaa gaagcagaag aattatgcag ttttgaagaa gtgcaagcag
      601 aacagctatt tgcgacttgg tctgaggctt ttcacaacat tgcgattgac ctttcctgtg
      661 taggaaaaaa acaactgatt tttttatgca cacaacatac caatctcctc agacgtcctt
      721 tgattattat cgatgaagtt ctatttattg gatacaatga gcagttgatg gaacagctta
      781 tcttaaataa atagacttta actgttttaa tcaagtcact gagcatttca tacagttgta
      841 acagtcgaac acttagcatc aagcgtgtta tgtgttcttt ttttgacttt atttttctac
      901 tatagagaca tgctcaattt atgtggtcaa aattcaaatc taggtgggga tatagagtta
      961 aaaatatatt gacatgtgta aatttacatg atatattcat ttctgttgtt ttaagtagca
     1021 aagcaataaa atagtatttt aaaaaacata taaaatagtt gttgacatgt tagaaatgac
     1081 atgataagat aactgagttg ttaaaaaaga accacttgtg aagcttgatt ctatcgaaaa
     1141 aaacttcaaa aaaagttctt gacaaataac aaatgataag ttatgataaa tgagttgctg
     1201 taagatttcg aaaaacgaaa tcaaaaaaac ttttaaaaaa gagttgacaa ttacttgtct
     1261 ctctgatatg atataaaagt tgctacggca gccaaaaacg aatcggggaa cactcctgat
     1321 gaagtagacc tttgaaaact gaacaaagta agacaaacca aatgtgtagg gcgtcttgat
     1381 tcaattcaag acaacaaaca ttttttaaca agcaagcaat atgctagcaa acaaattgag
     1441 cttaacaatc g
//
LOCUS       DAMYXM010000181         1424 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.187, whole genome shotgun sequence.
ACCESSION   DAMYXM010000181 DAMYXM010000000
VERSION     DAMYXM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.187"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(150..1337)
                     /locus_tag="PNS21_002899"
     CDS             complement(150..1337)
                     /locus_tag="PNS21_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HDI5160517.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DAMYXM010000182         1368 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.188, whole genome shotgun sequence.
ACCESSION   DAMYXM010000182 DAMYXM010000000
VERSION     DAMYXM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.188"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            82..>1368
                     /locus_tag="PNS21_002900"
     CDS             82..>1368
                     /locus_tag="PNS21_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HDI5160518.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          467 a          263 c          257 g          381 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaat
//
LOCUS       DAMYXM010000183         1357 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.189, whole genome shotgun sequence.
ACCESSION   DAMYXM010000183 DAMYXM010000000
VERSION     DAMYXM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.189"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            241..795
                     /locus_tag="PNS21_002901"
     CDS             241..795
                     /locus_tag="PNS21_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI5160519.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            1049..>1357
                     /locus_tag="PNS21_002902"
     CDS             1049..>1357
                     /locus_tag="PNS21_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160520.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     L"
BASE COUNT          550 a          142 c          239 g          426 t
ORIGIN      
        1 aacttctacg gataattgcc cgtctaaaat ttaaataaga ggttagaatc ttcatataag
       61 taaggttaat ttttttgtac aaaaaagtcg gaatattctg agttactgaa atgatataaa
      121 atcataaact aaaccccttg attttcttca cttttaacag agtatatata taagttcatg
      181 attatggtaa aatatatata taatcatgaa ctcagaatat ttttaagaag ggaaacaata
      241 gtgagctata atgtacaacc gttaaggaca aatgaagaga tacaagattt tcagttttgg
      301 ctaagaagaa caagtaatcc agaaagagat agttttttat ttttgtttgg aatcaataat
      361 gggttacgta tgtcagatat tataggatta aaagttggag acatacgagg taaaagtaag
      421 cctattattg ttgaacataa aacaggtaag cgaaaaccga tttttataga taatttgaga
      481 gaagagattt tattatatac tgaaggtaaa gaagaaaatg attggttatt tccaagtcgg
      541 caacaaggtc gacatataac aagggatcgt gtctatcaga tatatgcaga tatagcagaa
      601 aaattaggaa gagatgatat aggaacacat acattaagaa aaacttttgg gtatcactac
      661 tacaagaaaa ctagagacat tgctacctta atgtttattt ttaatcatag ctcgcaagca
      721 attacaaaaa ggtacattgg tataactgaa gatgaaatag gggcatcgtt acgtggtttt
      781 aaattgggag gctaattgag atattaggat atattttttg tgtataatga aaacttagaa
      841 ggtgagatta atgaagataa ctgaattttc ggaagtattt aaaacgttta gtaacattta
      901 caatagaata ccagagatag aacaaaatgt tgtagttgat aatgattatt atatagagaa
      961 tgattttaat aaaccaagaa aatatgacaa cctatacaat tttatacaca atagaacaac
     1021 aataaataac aacagtaaca ctcgagtttt gattaattca gcatattttc atattaaata
     1081 tttaaagaaa tatgagtatg aagaagttgt attagattat gggctaaata gacagtcatt
     1141 tttaatttat gcaataaata acggaaattt gccaagcaat aaaaagaaca taattattga
     1201 aacatatgcc tatctggagg ggggatctaa aaaactaatt aagtcatgta atatagagta
     1261 tcaattatca gtaggagaaa ttaaatgtgt tttcgaagaa gatttagaac ttgacttaaa
     1321 aaaaattttt gccagcattc atgattatat aggtttg
//
LOCUS       DAMYXM010000184         1355 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.190, whole genome shotgun sequence.
ACCESSION   DAMYXM010000184 DAMYXM010000000
VERSION     DAMYXM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.190"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..1073
                     /locus_tag="PNS21_002903"
     CDS             <1..1073
                     /locus_tag="PNS21_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDI5160521.1"
                     /translation="LQAELTAFLDYEKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTI
                     PRDRNGEFSQQTLPAYKRTNDSLETTIIQLFQKGITMAEISKLIEKMYGHHYTPQTIS
                     NMSKLVAEDVLAFKERTLEANYSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEV
                     LGFTIAPTESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCV
                     HVSRNIAHKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLN
                     PALLTFYNFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYN
                     QKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          473 a          227 c          224 g          431 t
ORIGIN      
        1 ttctacaagc agagctaact gcttttttag attacgaaaa atatgatcga aatggattca
       61 attcaggaaa ttcccgtaac gggaattatt cacgtacatt taaaacagaa tatggcgagt
      121 tgaatttaac tattcctaga gatcggaatg gcgaattttc tcaacaaaca ctaccagcat
      181 ataagcgaac caatgattca ttagaaacga ccattatcca attatttcaa aaaggcatta
      241 ctatggctga aatctccaaa ttaattgaaa aaatgtatgg tcaccactat acgccacaaa
      301 caatctccaa catgagtaaa ttggtggctg aagatgtttt agcttttaaa gaaagaacgt
      361 tagaagccaa ttattccgtt atatttatgg atgcgacgca tatccctgtg aagcgacaga
      421 ctgtttcgaa ggaagcagtg tatattacga ttggtattcg tttggatgga accaaagaag
      481 ttcttgggtt tactattgcc ccaactgagt ctgcttatat ttggaaagaa gttcttcaag
      541 atcttagaaa acgtgggtta gaagaagttt tattagtagt gacagacgga ttaagctgta
      601 ttgaagaaag tatccatagt gtgtatccga atgcccaatt tcaacaatgt tgtgtgcatg
      661 tatctagaaa tatcgctcat aaagttcgtg ttcgaggtcg aaaagaaatt tgtgaggatt
      721 tcaaattggt ttaccaagcg aattcaaaag aagaggcatt ggatcacatc gactttatga
      781 ctaggaaatg gaaaaagcag tatccaagag tcgtcaattt actcttgaat cctgccctat
      841 taacctttta taatttccct cacgccatca gacgaacaat ttattcgacg aacctgattg
      901 aaggctttaa taagcagcta aaacgatata ctcgaagaaa agaacaattc cctaatgaag
      961 aatctctaga gagattcttc atttctcaat ttaatcaata taaccaaaaa tttttaggta
     1021 gaattcacaa gggatttaaa gaaattcagg atacattaga gtcgatgatt taactgtaat
     1081 taacagaatg gattttccat ttacatataa ttcttgacgc taccataatc taaataacca
     1141 tcatattcat ctactactta tataattgag ttttattaaa ctttttaagt ttctattcta
     1201 tcaaatattt aaacaatctc tgcatccgaa aagactacct cgtatagttt tactaacaat
     1261 atttttatta gcgtgttcaa atcctgattt cttgtatcaa ttattttctg ttcactttca
     1321 ataagaaaaa agtctttact tccatggata ataat
//
LOCUS       DAMYXM010000185         1344 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.191, whole genome shotgun sequence.
ACCESSION   DAMYXM010000185 DAMYXM010000000
VERSION     DAMYXM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.191"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>1344
                     /locus_tag="PNS21_002904"
     rRNA            <1..>1344
                     /locus_tag="PNS21_002904"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          352 a          294 c          414 g          284 t
ORIGIN      
        1 tcctgtaccc gttgtttttg tttgagcaat ggagggacgc aggaggctaa ggaatgcaga
       61 cgatcggaaa tgtctgtcca agcagtaagt ctgaagagga gtcaaatgct tcttttctta
      121 aggacaagct gtgatgggga gggaaataat agtaccgaag ttcctgatgt cacactgccg
      181 agaaaagctt ctagtgagaa aacagcggcc cgtaccgcaa accgacacag gtagtcgagg
      241 agagaatcct aaggtgagcg agagaactct cgttaaggaa ctcggcaaaa tgaccccgta
      301 acttcgggag aaggggtgct gatcatacga tcagccgcag tgaataggcc caagcgactg
      361 tttatcaaaa acacaggtct ctgcaaaatc gtaagatgaa gtataggggc tgacgcctgc
      421 ccggtgctgg aaggttaaga ggagtgctta gcgcaagcga aggtacgaat tgaagcccca
      481 gtaaacggcg gccgtaacta taacggtcct aaggtagcga aattccttgt cgggtaagtt
      541 ccgacccgca cgaaaggcgt aacgatttgg gcactgtctc aacgagagac tcggtgaaat
      601 tttagtacct gtgaagatgc aggttacccg cgacaggacg gaaagacccc atggagcttt
      661 actgtagttt gatattgagt gtctgtaccg catgtacagg ataggtagga gccgtagaaa
      721 tcggaacgct agtttcgatg gaggcgctgg tgggatacta cccctgcgtt atggccactc
      781 taacccgcac cactaatcgt ggtgggagac agtgtcagat gggcagtttg actggggcgg
      841 tcgcctccta aaaggtaacg gaggcgccca aaggttccct cagaatggtt ggaaatcatt
      901 cgaagagtgt aaaggcagaa gggagcttga ctgcgagacc aacaagtcga gcagggacga
      961 aagtcgggct tagtgatccg gtggttccgc atggaagggc catcgctcaa cggataaaag
     1021 ctaccctggg gataacaggc ttatctcccc caagagtcca catcgacggg gaggtttggc
     1081 acctcgatgt cggctcgtcg catcctgggg ctgtagtcgg tcccaagggt tgggctgttc
     1141 gcccattaaa gcggcacgcg agctgggttc agaacgtcgt gagacagttc ggtccctatc
     1201 cgtcgcgggc gttggaaatt tgagaggagc tgtccttagt acgagaggac cgggatggac
     1261 ttaccgctgg tgtaccagtt gttctgccaa gggcattgct gggtagctat gtagggaagg
     1321 gataaacgct gaaagcatct aagt
//
LOCUS       DAMYXM010000186         1336 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.192, whole genome shotgun sequence.
ACCESSION   DAMYXM010000186 DAMYXM010000000
VERSION     DAMYXM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.192"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="PNS21_002905"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="PNS21_002905"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDI5160522.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          459 a          180 c          223 g          474 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta acttattgct tttgcaaaat ttatttacct gttttctgga tcaacgatag
     1141 tgtatattat gctatccttg tttggatttt aggacgttta aagaggcact tttttacata
     1201 acttaatcca tagacctttt aagcatgctg tgagataata ataaagaaga gtttaaagcg
     1261 caccccaaac cacttcccca taagtgtatt acgctttaaa ctcttttata tttgaattca
     1321 ctaagaagca taccat
//
LOCUS       DAMYXM010000187         1309 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.194, whole genome shotgun sequence.
ACCESSION   DAMYXM010000187 DAMYXM010000000
VERSION     DAMYXM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.194"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            35..532
                     /locus_tag="PNS21_002906"
     CDS             35..532
                     /locus_tag="PNS21_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5160523.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRIYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(529..1263)
                     /locus_tag="PNS21_002907"
     CDS             complement(529..1263)
                     /locus_tag="PNS21_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160524.1"
                     /translation="MRSSKYTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVL
                     KGIEIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFA
                     QLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKS
                     AYLYDNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKAKGVTELAI
                     YQKGKRIMVPLQSKKTSSASKVRTFF"
BASE COUNT          453 a          219 c          229 g          408 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttatatgaac aatcaaatca cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gatctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 ccctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactgtaat ggaaccataa ttcgctttcc
      601 tttttgataa atcgctaatt ccgtcacacc ttttgctttt aacagagcaa aaagatcatc
      661 gaaatggaaa cggaaatgtt caaggtaatg cccatctgat ccaacactaa acaaggtacc
      721 accacgtgaa agatataaat cgatgacata ttcatataac gctaaattgt catacaagta
      781 agcgctctta gcatttaatt cgaatgccaa tttatttttg attacttgat ctagaatcgg
      841 taaaaactgc gcttcatatt gtttaaattc agcaacggat agaccaaata cccgaaaacc
      901 atagtcaaaa tgagcaaaga catctgcttc tataacaact agtgcttctg aaagttgtgc
      961 aaaatactga ggaatcacaa tagcaggatc catatctttg acttcgtcat ctaaataatc
     1021 aaattgacca ttatgatgca cactgagtaa ttttaaatcg taatctttat cggctaaata
     1081 gtcgatgatc cgatcctttt cagaagcgat gtaaccgatt tcgatgcctt ttaaaacacg
     1141 attgccaaaa cgcttattta agctatcgat tgtttgactg tacatttcat aatcggggat
     1201 atcatcgact ttagtaacag gattggctaa gtcaaaatgt tcagtatatt ttgatgaacg
     1261 catatgtagt cctcatttct tgggagattt gagactacta tatgcgttt
//
LOCUS       DAMYXM010000188         1222 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.197, whole genome shotgun sequence.
ACCESSION   DAMYXM010000188 DAMYXM010000000
VERSION     DAMYXM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.197"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..567)
                     /locus_tag="PNS21_002908"
     CDS             complement(<1..567)
                     /locus_tag="PNS21_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDI5160525.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVK"
     gene            complement(567..>1222)
                     /locus_tag="PNS21_002909"
     CDS             complement(567..>1222)
                     /locus_tag="PNS21_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDI5160526.1"
                     /translation="KDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWH
                     PWEDYRNFFVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFT
                     LIIEHETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQ
                     LYDEVMHDNNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT          418 a          209 c          137 g          458 t
ORIGIN      
        1 ttttactgat ttagggaaac caaataggtt tataatataa tccaattcat gtatacagtc
       61 taattctact cctcccccca tagatttaaa ggcagaataa ttatttctat aatcagtacc
      121 ttttctccaa tcaggaagat aagtagaaca aatagctctt acagaatata cttttttact
      181 ttgaatacta ttacgaacaa cttccatgat atttttatac ctcagtggag cagcaacata
      241 aatatttttg taatcataat tatattgagt aagatcaata ctctcagaaa aagcaggttt
      301 ttctaaaaat atgtaatcaa ctttttcaac aattacattt agtgtttcca gatgcttaaa
      361 agtcggattt gtaataaaag ctatatcata atgtgtatcc aaatcgtctc tattttgaat
      421 atacgttatt tcttcaaact ctttttttcc actgctacta taagctgtta tatcaaaatt
      481 aatagaattc ttttttccat agcttattag attttttaga tgtctctttc caattgaccc
      541 taacccaaaa aaaattactt tcatcattaa tttacctcaa tgtcgttaat taagttaata
      601 atatacttat ctttttcaaa agtatattgg ggttccattt tcttttccaa aagcgtgaaa
      661 aattctttaa tctcctctaa atatgcatct tcaataatag tatgtgaata attattatca
      721 tgcataacct catcgtacaa ttgaatatct gtcatactag aagtttcttc attccatgaa
      781 aatagagaat ccgggttacc ttcccattct attaaaatct tttcaccaat tatagttaaa
      841 tttcttttag ctactcttga aacaacatct acacttaaag ttccaactat accagtttca
      901 tgctcaataa taagagtgaa actgtccgaa taatcaatat caagattgga taatttctgg
      961 gtcattacag taaacgattt aactttccca aatgtatcta taatccaagg taattcaata
     1021 gcgaatattt ctctacaccc attagttttt ttgtttgaaa caaaaaaatt gcgataatct
     1081 tcccaaggat gccagtcggg taaatactgt cctacatggt attgataaga aattttttta
     1141 ttatgcgtca ctttagactt aatatattga atttcttttc tcttcaaaaa tgtagaagat
     1201 agataaagta atctatcttt ct
//
LOCUS       DAMYXM010000189         1195 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.198, whole genome shotgun sequence.
ACCESSION   DAMYXM010000189 DAMYXM010000000
VERSION     DAMYXM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.198"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..1076
                     /locus_tag="PNS21_002910"
     CDS             <1..1076
                     /locus_tag="PNS21_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDI5160527.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAMYXM010000190         1163 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.199, whole genome shotgun sequence.
ACCESSION   DAMYXM010000190 DAMYXM010000000
VERSION     DAMYXM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.199"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            20..280
                     /locus_tag="PNS21_002911"
     CDS             20..280
                     /locus_tag="PNS21_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160528.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(642..881)
                     /locus_tag="PNS21_002912"
     CDS             complement(642..881)
                     /locus_tag="PNS21_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160529.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          409 a          164 c          217 g          373 t
ORIGIN      
        1 cattaaggat ggatgaaata tgaataacga aactatcatt ttaggaaaaa aatatatttg
       61 ccaacctatt ggcttgaaac aaaaagtggt tggagaagta atcagtaaat tagaaaattg
      121 tgttgtgatc tgcgtagacg aatatcatga cgtcgatcgc aatgaaatat tagaaaaatg
      181 cggaaaagta gtggcaaaat atactcacgt ttatgagttg gaaacaacag aatatctata
      241 taactatagc aacaaatatg aacatacttt agtttcataa agatttaaat aaagtgcctt
      301 caaaagcgag aagaccaagc ttttgaaggc actttattta gttggcagtg attgttcatg
      361 atttttgatt tattcaaaag gttcgcctag agaatctgtc tctgtaacga ggcctggtgg
      421 agaaactcga taaaaaccta tactgccaga actgtcctat tttcggatag acaaactagt
      481 agttttaaaa agatcatccg atccaatcat ttcaaaaaat gcaaaactga tatcaggatc
      541 atttagctca ggcttgttat cgtaaagcat ttgtaaaggt tgcatttagt taggagtaaa
      601 cgcgaaacta tcatcattat ttgctgaaga agatgaagaa gctaattcag agtttgtttg
      661 aaataaagga gacgtgctga cagtttcttt accctgtttt ttgcagaggt agaaactttt
      721 atatcggaag tttctaccgg agaatttttc tcatttgtag aaattgttga atcgatacta
      781 ttcgaaattt gcgtatctga aggtatgttc tttgatgcag aagtcccaca agcagtaagt
      841 aacaataaat ttgataaact gataaacaga attcttttca ttatgtacac actccttata
      901 gtatcgttta gcatacacct atgctcagta ttaggcaaaa tcaatgcaaa actatcaagt
      961 cttttatagt atctattagt cttagtaata catatatgga gagggtgata tcccagttca
     1021 aaccagatac attggttacc atctgtatta acgattatgc ctctaaacgc tttttttatg
     1081 aaaaaattta taagtgaaaa cgtaggtatg atattatcta gaggtaattt ttttgggtat
     1141 ttaaatagtt gctaggagga tag
//
LOCUS       DAMYXM010000191         1085 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.201, whole genome shotgun sequence.
ACCESSION   DAMYXM010000191 DAMYXM010000000
VERSION     DAMYXM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.201"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            2..>1085
                     /locus_tag="PNS21_002913"
     rRNA            2..>1085
                     /locus_tag="PNS21_002913"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          332 a          215 c          318 g          220 t
ORIGIN      
        1 ataagggcgc acggtggatg ccttggcact aggagccgat gaaggacggg actaacaccg
       61 atatgctttg gggagctgta cgtaagctat gatccagaga tttccgaatg ggggaaccca
      121 acatctttaa taggatgtta cgattgtgtg aatacatagc acattcgagg tagacgcaga
      181 gaactgaaac atctaagtac ctgcaggaag agaaagaaaa ttcgattccc tgagtagcgg
      241 cgagcgaaac gggaaaagcc caaaccagca agcttgcttg ttggggttgt aggactccaa
      301 tatggtagtt ctttcagata gtcgaatgac ttggaaaagt cagtcaaaga gggtaaaaac
      361 cccgtagacg aaatgtggaa gacacctagg aggatcctga gtacggcgga acacgagaaa
      421 ttccgtcgga atccgggagg accatctccc aaggctaaat actccctagt gaccgatagt
      481 gaaccagtac cgtgagggaa aggtgaaaag caccccggaa ggggagtgaa atagaacctg
      541 aaaccgtgtg cctacaacaa gtcaaagccc gttaatgggt gatggcgtgc cttttgtaga
      601 atgaaccggc gagttacgat tgcatgcgag gttaagttga agagacggag ccgcagcgaa
      661 agcgagtctg aatagggcgt ttgagtatgt agtcgtagac ccgaaaccat gtgatctacc
      721 catgtccagg ttgaaggtgc ggtaaaacgc actggaggac cgaacccacg tacgttgaaa
      781 agtgcgggga tgaggtgtgg gtagcggaga aattccaaac gaacttggag atagctggtt
      841 ctctccgaaa tagctttagg gctagcctcg gaattgagaa tgatggaggt agagcactgt
      901 ttggactagg ggcccatctc gggttaccga attcagataa actccgaatg ccattcattc
      961 atatccggga gtcagactgt gagtgataag atccatagtc gaaagggaaa cagcccagac
     1021 caccagctaa ggtcccaaaa tatatgttaa gtggaaaagg atgtggggtt gcacagacaa
     1081 ctagg
//
LOCUS       DAMYXM010000192         1076 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.202, whole genome shotgun sequence.
ACCESSION   DAMYXM010000192 DAMYXM010000000
VERSION     DAMYXM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.202"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(201..>1076)
                     /locus_tag="PNS21_002914"
     CDS             complement(201..>1076)
                     /locus_tag="PNS21_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HDI5160530.1"
                     /translation="RLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYT
                     ENLSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIP
                     SVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKV
                     RDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRES
                     FKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSP
                     KQYQLGLVDNFNNIS"
BASE COUNT          371 a          186 c          123 g          396 t
ORIGIN      
        1 cttcacataa actttttact ttattaatta ctactttgtc atattcattt aacacactat
       61 tattaccatt acttagacgc tcagattcta cgaaacaact caaatcagaa atttcccaaa
      121 aaaatttttt gttaaaatcc aatttcctca actcctccaa actacgtaaa tgatatagtc
      181 tcataataaa ataaaacctc tcagctaatg ttgttgaaat tgtccactaa acctaattga
      241 tattgcttag gagatacacc tgcgtacttt ttgaatactc tcgaaaagtg tgaagaatct
      301 gaaaatccta aacctaaaga aacttcactg atagtatggt ttgatttcaa cattatttta
      361 gccgtagaaa tttttcgctt agagatataa ttataaatcg taatatgcat ttcattctta
      421 aagctttctc ttaatttagt ttcacttaca gaaaaataat tagctatttc ctttacagta
      481 attcttttat agatatttaa gccgatgaat tgaacgacag aagaaattaa gggagaaaga
      541 tgctctatct tagtttttcc gatttcctta gtataataaa caatcccaga atccctcact
      601 tttaagacct caggtaacga tacagctaat tcgttttctt gtatcaaagc atctctactt
      661 tgatacgctt ctattatatc cattcctaat gttatagacg tttgtgctaa cttttcaaaa
      721 acaatgatag aataattttt ttcagaccga atcgtatctc cactaacact tggaattatt
      781 ccattactca gattaaaaac catgttttct aaatttcgaa tatctccatt tttaacatat
      841 tccagtattt tattttcata aagaaacaga tatatttctg gatcgtaatt ttgtgataac
      901 agattatcta ttttgatttg ctcgatagtt ttacttagat tttctgtata ttcaattaac
      961 ggttcatgga atagatcttc tatttttcca gtaaaaaaat aatgaactaa aatcagaata
     1021 tctcgaatgt ctcctaaaga atacaaagtc aaattttgca atgaattata gagtcg
//
LOCUS       DAMYXM010000193         1062 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.203, whole genome shotgun sequence.
ACCESSION   DAMYXM010000193 DAMYXM010000000
VERSION     DAMYXM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.203"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(37..993)
                     /locus_tag="PNS21_002915"
     CDS             complement(37..993)
                     /locus_tag="PNS21_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160531.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          347 a          177 c          159 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaaa agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DAMYXM010000194         1035 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.204, whole genome shotgun sequence.
ACCESSION   DAMYXM010000194 DAMYXM010000000
VERSION     DAMYXM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.204"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..>1035)
                     /locus_tag="PNS21_002916"
     rRNA            complement(<1..>1035)
                     /locus_tag="PNS21_002916"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DAMYXM010000195          983 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.205, whole genome shotgun sequence.
ACCESSION   DAMYXM010000195 DAMYXM010000000
VERSION     DAMYXM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.205"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..653)
                     /locus_tag="PNS21_002917"
     CDS             complement(<1..653)
                     /locus_tag="PNS21_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDI5160532.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIK"
BASE COUNT          327 a          185 c          105 g          366 t
ORIGIN      
        1 tctttaataa aacttgttat ttttgaatgt tgcaaatatg aaatatggaa tatgatatct
       61 tcagatatat aatccctttc tgacggaaac ttgatattgt gctgttgaat tatctcagat
      121 gaatacagcg catgccaaac tgacattggc aatataaaat cgactttata actcggctca
      181 gtgccaatca ttccaagcaa cactttttca attatagcgt tatttgtaaa tatcgtacta
      241 tcatatcttg ctggctttga aataatatta ttatcatcat aatattcata atatccagca
      301 taacatgtgt ctgcattact cctttttata ccatttaaca ttgcttcaat ataattagct
      361 gcaacaaaat catcagaatc aataaaagca acatattttc cttttacatg ttcaagtcct
      421 gtatttcttg ccaatcccaa gccagcatta tttttatgaa taatacgaat ccttgaatcc
      481 tttaatgcat agtcttcaca tattttatta gatgaatcct tagatccatc atccacaatt
      541 ataatatcta ggttcttata tgtttgatta cacactgatt ccaaacaact tcctacatac
      601 ttttcaacat tatatacagg tatgattaca ctaattagat cactcgaatt catccttcta
      661 tcctttcact cacgttttct caattagtca atgacggcat caacgatttt cttaaattct
      721 ctgaaaaaaa tagactctga tcttgatgat gtgaatgaca tactatcttc ttctagaaaa
      781 attatttcct ttaaattatt tattaaatca ataatatgat cctcgtaagt atgctttccc
      841 ttggaataat gaaaatatac ttttgtatta cacaatattg caattttatt tcttaatatg
      901 attatttcac tcattcatta ctgccacata tacacaactt ataaccaccc cctgcaaagt
      961 ttagggtaag ttatcagata ata
//
LOCUS       DAMYXM010000196          977 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.206, whole genome shotgun sequence.
ACCESSION   DAMYXM010000196 DAMYXM010000000
VERSION     DAMYXM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.206"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            346..977
                     /locus_tag="PNS21_002918"
                     /pseudo
     CDS             346..977
                     /locus_tag="PNS21_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          332 a          180 c          178 g          287 t
ORIGIN      
        1 tagttatata tttaaatttt taccagaaag aaatgaacac cttcaattat ctaaatctcg
       61 aaaatggtta gatgtaatca tcaggaaaaa caatcttaaa cgaattacta ttcatggtct
      121 tagacacaca catgctagct tgcttttagc aagctagtgc aaatattaaa gatgtgcaag
      181 agcgtttagg ccactcttct attcaaatca ttatggatct atatattcat attacagaca
      241 aaagaaaaga agaaaccgta gcgcaattcg caaaatatag gctctataat ttatgggact
      301 gatgaatcaa aattgattct tcagtcccat tgttatttta ggtatatgat tcgtacattg
      361 aaagagttga aaaactatct tccgaagccg tcaaaaagga ccctgccaaa agtattgatg
      421 gtggatgaat ttcgttcgca cgcttcaatt gtggataaaa tgagctttat ttgtgcggat
      481 ggcgagactg gaaaactcat tgatattttg cctacacgaa aattgtctcg attaacacac
      541 tattttagtc gctgtgcaaa tcctgaagcg gtggaatatt tagtgacaga tatgaacgcc
      601 gcctacttcc aactgacaaa acgtgtctta ccaaatgcga aagtcgtcat tgattgtttt
      661 catatcatca aacatttgaa tcaggcgttc aatgattttc gtgtacaaga aatgaaaaga
      721 ttacaccagt tagggcaacg acgggaagcc gagaaagtga aaaagaactg gcgctttctt
      781 ctaaaaaacc gtcaaaaaat caatcactat gattacaaaa catggcgcag ttttcgtgca
      841 cctaaatacc cgctactcac tgaagcaatg atgattgatc gattgctggc attttcaacg
      901 cctttaaagg acgcctatac ttttttccat gagctgaccg aagcctttag agacaaggaa
      961 tcagacttat tttttac
//
LOCUS       DAMYXM010000197          945 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.207, whole genome shotgun sequence.
ACCESSION   DAMYXM010000197 DAMYXM010000000
VERSION     DAMYXM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.207"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..267
                     /locus_tag="PNS21_002919"
     CDS             <1..267
                     /locus_tag="PNS21_002919"
                     /inference="COORDINATES: protein motif:HMM:NF025015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDI5160533.1"
                     /translation="KINSFYGFRTKKSMKNEKSWHLAQKLLGDFSKKKVIPVFLMGIV
                     FFSIEIYSLAILKNENFFVSVLMGESLILILILCLIVHEVNKQL"
     gene            691..>945
                     /locus_tag="PNS21_002920"
     CDS             691..>945
                     /locus_tag="PNS21_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160534.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNY"
BASE COUNT          368 a           99 c          166 g          312 t
ORIGIN      
        1 aaaattaatt ctttttatgg ctttagaact aaaaagtcaa tgaaaaatga aaaaagttgg
       61 catttagcac aaaaattgtt gggggatttt tcaaaaaaaa aagttatccc agtatttttg
      121 atgggtattg tttttttcag tatagagatt tatagtttag ctattttaaa aaatgaaaat
      181 tttttcgtta gtgtattaat gggtgaaagt ttaatcctaa tactgatttt gtgtttaatt
      241 gttcatgaag tgaataaaca attgtgaatt aaaaaaataa aaacctagta taatcacttt
      301 aataaattgt tggtagaagg tgatcaaaat gggagtaaac gaaatgatga aagtcagatt
      361 gagtttttaa atgatacctt cctcagaact tcaaaacgaa ttcaaagatt cgttgacaaa
      421 tcttgagcaa gatcatttaa aaaaatattt tctcttcatt tgacgaaaaa tgtttagaaa
      481 tcttatataa gtgaagataa agtaattggt gtaattaatt gtagagaata gcctttgagc
      541 aattgaagcg atacaattat ttagaaaatg aacttcctcc agcatactac atgattcctt
      601 acgggacgat gttaaagaag aaagcaagaa attttagaag tattagtaga taaactaatg
      661 ggcttttctc aatttaaggg agtagcccaa atgtttatca aatatggtgc agatacagta
      721 gcaaccatgt ttacagaaga atatcaagta gactattatg aagtaataga taaatgtgta
      781 cattttgttt atgaaaaagt gtggaatttt atgaattttg cgataagtga agaaatctta
      841 cgagtacaag aaaacagaac aggaatattt accagagaca gaaagcttaa tttgattgcg
      901 cattatatac ggaatcgctg gtcatcaata ccaaatttta attat
//
LOCUS       DAMYXM010000198          925 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.208, whole genome shotgun sequence.
ACCESSION   DAMYXM010000198 DAMYXM010000000
VERSION     DAMYXM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.208"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            92..>925
                     /locus_tag="PNS21_002921"
     CDS             92..>925
                     /locus_tag="PNS21_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDI5160535.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QR"
BASE COUNT          277 a          184 c          175 g          289 t
ORIGIN      
        1 accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc tttagaatta
       61 atttggctac caattcatag gaggattcaa gatgtctcat actcagctta tcaaagatac
      121 cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta aagaaaaata
      181 caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag aagagtgttc
      241 ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa ccgttcggtt
      301 gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac aacgatttaa
      361 atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa aacactgttt
      421 cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata cttccatgac
      481 ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca tgaagcgatt
      541 ctaccgccca ttgaatcctt tcaaacagac tttgccaaag gtcttatgct ttgatgaatt
      601 caaatcggtt cgaagtgttg ccagtgcaat gagttttatc atgatggacg gacaaagcca
      661 tgcattattg gatattgtcg aaaatcgacg cttgccttat ttagaacgct atttttctcg
      721 gttttcatta gcgactcgtg aagccgttca attgattgtt attgatatgt atgcgcctta
      781 tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc gtttccacat
      841 cgtccaacat attggcagaa ctttcttgaa tcatcgagtg aaagaaacca cggtacgact
      901 taaaggaaat tcccactctc aacga
//
LOCUS       DAMYXM010000199          908 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.209, whole genome shotgun sequence.
ACCESSION   DAMYXM010000199 DAMYXM010000000
VERSION     DAMYXM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.209"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..146)
                     /locus_tag="PNS21_002922"
     CDS             complement(<1..146)
                     /locus_tag="PNS21_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160536.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGR"
     gene            complement(248..601)
                     /gene="tnpB"
                     /locus_tag="PNS21_002923"
     CDS             complement(248..601)
                     /gene="tnpB"
                     /locus_tag="PNS21_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDI5160537.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(591..785)
                     /locus_tag="PNS21_002924"
     CDS             complement(591..785)
                     /locus_tag="PNS21_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160538.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          266 a          180 c          160 g          302 t
ORIGIN      
        1 cgtccaaata atttgttggt taaataattg accttttcat tcaatagttg gatttcagtt
       61 gtaagattct gaatggtttg ttgttgtgct tgattggttt tatgggcttc ttgaagcaac
      121 tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa ctgattttgt
      181 tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt tgtgttaacc
      241 ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga tcgatactta
      301 atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg tctttcgtcc
      361 gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa ccatcaccgt
      421 cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa acggcatctt
      481 cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca attccttttc
      541 gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa tcataaagca
      601 ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc gttatagatc
      661 aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc cgcgggttta
      721 cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc atttaattta
      781 tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag
      841 taaggtactg ttctatgggg cgcttacttt ataaaggtcg tccaaaactc tatagtgcag
      901 atacaaaa
//
LOCUS       DAMYXM010000200          890 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.210, whole genome shotgun sequence.
ACCESSION   DAMYXM010000200 DAMYXM010000000
VERSION     DAMYXM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.210"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..888
                     /locus_tag="PNS21_002925"
     CDS             <1..888
                     /locus_tag="PNS21_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HDI5160539.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DAMYXM010000201          880 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.211, whole genome shotgun sequence.
ACCESSION   DAMYXM010000201 DAMYXM010000000
VERSION     DAMYXM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.211"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..231)
                     /locus_tag="PNS21_002926"
     CDS             complement(<1..231)
                     /locus_tag="PNS21_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HDI5160540.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMI"
     gene            complement(224..>880)
                     /locus_tag="PNS21_002927"
     CDS             complement(224..>880)
                     /locus_tag="PNS21_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDI5160541.1"
                     /translation="NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYP
                     RINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMY
                     EQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSK
                     LANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE
                     "
BASE COUNT          308 a          139 c           96 g          337 t
ORIGIN      
        1 aatcattgct ccaatcagta gccaagaaaa acgcatttta aaaaaatttt tctttataaa
       61 aacctcatac aaaactataa ttccacccca agcaaaagct attttcatcg ggatattagt
      121 tacattaaaa gtactaggca taaaataaag cagacaaatg attaaattta ttactcgaaa
      181 cgcatctcga cttaataaac tcttctctaa aacaggtact attctattca tctatatttc
      241 caactttcaa aatttcttta tcaattctat ttaatagttt atcagagtaa tccgatttaa
      301 cttttcttac aaataatttt tcactttctt ttatttgatc tatatccctt tcatcaaacc
      361 atttgttcat ggaaggatca ataatatgaa tatttgctaa tttcgaagtt tgtaaagtag
      421 tttcttcaat atttttcaat tcaagtggta aatctggtcc tccaataaca tttttatttt
      481 tgaacgagga attaaaaaaa agtgtatgaa aaaatttttc atctggggca aacataccta
      541 ttttaaaatt caggtagtct tgttgatttt gttcatacat ttgtattaat tctgttaggc
      601 attctttcga caaagcccac cattgagaac catgatacaa atcaaattta tggtgtctaa
      661 tttttggggg aagtttaatc atcctcagac aacgattaat tatagctcta aaaattttaa
      721 atgcagttgt gttattccta ttaattctag gataatcatg tttttgatat acatctatat
      781 gtttataata aaattcttta tgtttaattt gatctagatc tattccacga ataaactcca
      841 ctgaactatt ctttttaaaa taattataaa tatactcatt
//
LOCUS       DAMYXM010000202          837 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.212, whole genome shotgun sequence.
ACCESSION   DAMYXM010000202 DAMYXM010000000
VERSION     DAMYXM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.212"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            203..733
                     /locus_tag="PNS21_002928"
     CDS             203..733
                     /locus_tag="PNS21_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160542.1"
                     /translation="MISDNKWEINLKRFISEYIYTIETKSSFSLNEDSDPLKKYTIQC
                     KKNKDEKLFVIRPDLMKSNTHYIKEKPKDCDYIIISEANKTVFLIELKSSAQTSPAKE
                     ISEQIDGGYKWLQHLSFLTKYDFTEFSYKIIKVCCKFEARQDRKNLFKPNEYGIYNMN
                     GPKLDLRELYRKCNDL"
     gene            complement(728..>837)
                     /locus_tag="PNS21_002929"
     CDS             complement(728..>837)
                     /locus_tag="PNS21_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5160543.1"
                     /translation="NIVKDLNEGVSISKIAKNYNVTRQTIYRIKKDLNL"
BASE COUNT          335 a           98 c          129 g          275 t
ORIGIN      
        1 catgatgaat ggatttattc ttcaagaaaa tacatcaatt ttgataagta gaaatggtaa
       61 aaacattgta tagcatttta cacaggagtc tggacttgac taaaatttct aacatcagtt
      121 attatgataa ttttgatgat gtcaattatg atttacagga aagatataat gatttaatcg
      181 aagaattaga ggagagagga gaatgatttc agataataaa tgggaaataa atctcaaaag
      241 atttatttca gaatatatat atacgattga aactaaatct agtttttctc ttaatgaaga
      301 cagtgatcct ctaaaaaaat acactattca atgtaagaaa aataaagatg aaaaattgtt
      361 cgtaataaga ccagatctta tgaagagtaa tacccactat ataaaagaaa aacctaaaga
      421 ttgtgactac atcatcataa gtgaagcaaa taaaacagta tttctgatag aacttaaaag
      481 ctcagctcag acttcccctg caaaagaaat tagtgaacaa attgatggtg gatataagtg
      541 gttacaacat cttagttttc taacaaaata tgattttact gaattcagtt ataaaattat
      601 taaggtatgt tgtaaatttg aggcaagaca agatcgtaaa aatcttttta aacctaatga
      661 atatggtatt tataatatga atggtccaaa attagactta agagagttgt atagaaagtg
      721 taacgatcta taaattcaaa tcttttttaa ttctatatat tgtttgtcta gtaacgttat
      781 aattctttgc aatctttgaa atagagacgc cctcattcaa gtccttaacg atgtttt
//
LOCUS       DAMYXM010000203          804 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.213, whole genome shotgun sequence.
ACCESSION   DAMYXM010000203 DAMYXM010000000
VERSION     DAMYXM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.213"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>804
                     /locus_tag="PNS21_002930"
     CDS             <1..>804
                     /locus_tag="PNS21_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HDI5160544.1"
                     /translation="KRQVIDASKHLYPISAMCRILGLSRQSYYYQSKPKKDESELEEV
                     VAEEFIRSRKAYGSRKIKKALSKRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTC
                     NEAKTTNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHK
                     DAVLVKKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNA
                     VAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYET"
BASE COUNT          285 a          142 c          154 g          223 t
ORIGIN      
        1 cgaagagaca agtaatcgat gcgagtaagc atctttaccc tatatcagcg atgtgcagaa
       61 tattaggtct atcacgtcag tcctattatt atcaatcaaa accaaagaaa gacgaatcag
      121 aacttgaaga agtagtcgct gaagaattta tccgcagccg aaaggcctac ggctcaagaa
      181 aaataaaaaa agccttatca aaacgaggca ttcagatcag ccgacgaaaa attagtagaa
      241 tcatgaaaaa tagaggatta aaatcgagct atactgttgc ttattttaaa gtacatcatt
      301 ctacttgcaa tgaagccaaa acgacaaacg tattgaatcg taaattctta agagacaacc
      361 cattagaagc gatcgtaaca gacttgactt atgtacgagt cgggaaaaaa tggaattatg
      421 tctgtttcat tttggatctg ttcaatcgag aaattctcgg ctattcttgt ggagaacata
      481 aagatgccgt tctagtaaaa aaagcattta gccgtatcaa acaacctctg acagaggttg
      541 agatttttca tactgatcgt ggaaaagagt ttgataacca agctattgat gaattattaa
      601 caacttttga catcaatcga tcattgagtc ataaaggctg tccttttgat aatgccgtag
      661 ctgaatcaac ttataagtcg ttgaaagtag aatttgtcta tcaatacaca tttgaaacct
      721 tacaacaatt ggatttggag ttatttgact atgtcaattg gtggaaccac cttcggttgc
      781 acggtacact tggctacgag acac
//
LOCUS       DAMYXM010000204          786 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.214, whole genome shotgun sequence.
ACCESSION   DAMYXM010000204 DAMYXM010000000
VERSION     DAMYXM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.214"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(51..>786)
                     /gene="aph(2'')-I"
                     /locus_tag="PNS21_002931"
     CDS             complement(51..>786)
                     /gene="aph(2'')-I"
                     /locus_tag="PNS21_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HDI5160545.1"
                     /translation="GYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKG
                     TFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRE
                     TIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFG
                     DSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETI
                     VYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          266 a          128 c           53 g          339 t
ORIGIN      
        1 aactatttaa tactaatgtc ttttataata gcttttcata tattatataa tcaatcttta
       61 taagtccttt tataaatttc ttttctacca ttttcgataa attcctgttt aatattttta
      121 attccataaa caatagtttc aataggataa tattcttcaa ctatatcttg atattctttt
      181 gctttctcaa tatctatatt tccatacatt cttaatatat cttctccaaa atttgttcct
      241 atttcttctt cactatcttc aagtaagtat ataaaatcac aatattcatc tataattcca
      301 gaatctccaa aatcaattat tccagttaat ctattattgc catctaacaa tagatgatta
      361 caactaaaat cattatggca taaacacttt ttaccctcaa aaactgttgt tgcatttagt
      421 ctttccataa aactttctat ataatctttt tctatatcag ttaaatcatt ataaatagtt
      481 tcacgcaaca atatatactc ttctaataca ttttgtttat tatcaatagt acattcacta
      541 atatctgtat aatctaaacc gtgcatttgt cttaaaaaac tggcaatatc tcgttttaac
      601 aaattttgtt cttcttctga catagtagaa taaatttctg gtgttaaaaa agttccttta
      661 atttctttat aacctagtat agataattca tcactaatat acgaatattc aatattagga
      721 atttttacat tagtttctaa atttgtattt aaaaaattat atattgcttt ttcttttgca
      781 taacct
//
LOCUS       DAMYXM010000205          748 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.215, whole genome shotgun sequence.
ACCESSION   DAMYXM010000205 DAMYXM010000000
VERSION     DAMYXM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.215"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            554..>748
                     /locus_tag="PNS21_002932"
     CDS             554..>748
                     /locus_tag="PNS21_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI5160546.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYY"
BASE COUNT          271 a          136 c          115 g          226 t
ORIGIN      
        1 gacactcctc ttaatataca gtcctattgt ataaagtgtt actatataca tagtaactgt
       61 atacataaaa aaagtcaagt attagaaaaa agttcaaaag atcaagtgtg cgcttcgctc
      121 ccactcccct cgacgtttta gtagcctttc cctcacttcg ttcagtccaa gccaactaaa
      181 aagttttcgg gatacattct ccttctcttc ctaataatca attaaaattt taatctttat
      241 atttctgcta atcactcagg aaacagtaca gaaaaacaaa cacgtatcat agatataaat
      301 gtaatggcat agtgcgggtt ttattttcag cctgtatcat agctaaacaa atcgagttgt
      361 gtgtccgttt ttgggcgttc tgctagcttg tttaaagtct cttgaatgaa tgtatgttct
      421 aagtcaaaaa actctgacag cgcctttata tagataaagt ccgtataatt gtgtaaaagt
      481 aaaaaggcca tataacagtc cttttacggt acaatgtttt taacgacaaa aacataccca
      541 ggaggacttt tacatgaccc aagtacattt tacactgaaa agcgaagaga ttcaaagcat
      601 tattgaatat tctgtaaagg atgacgtttc taaaaatatt ttaacaacgg tatttaatca
      661 actaatggaa aatcaacgaa cagaatatat tcaagcaaaa gaatatgaac gaacagaaaa
      721 ccgacaaagt caacgaaatg gctattat
//
LOCUS       DAMYXM010000206          719 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.216, whole genome shotgun sequence.
ACCESSION   DAMYXM010000206 DAMYXM010000000
VERSION     DAMYXM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.216"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>719
                     /locus_tag="PNS21_002933"
     CDS             <1..>719
                     /locus_tag="PNS21_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HDI5160547.1"
                     /translation="EDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFTKEMTH
                     DEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGEREK
                     QFAYSAHTSCDDNGFILSTIITPGNIHDSQVAFQLVKQSKRLFPEINCVVADAGYKTP
                     KFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTRER
                     YREYKSNPKECVNCPLLNQCT"
BASE COUNT          243 a          136 c          141 g          199 t
ORIGIN      
        1 tgaggatatt tttgcctata ttttagaaca agcagttaaa gcaggttttg taacagagga
       61 taatttgtat ctggattcta cccacatcaa agctaacgcc aataaacata agtttacgaa
      121 agaaatgacc catgatgaag cgaaagccta tcaggatgaa ttagaagatg agatcaatcg
      181 tcaacgaata gaggctggaa aacgcccttt tactttggac ttagaaaagg aagtgaagct
      241 gaaagaaaga aaaataagta aagctgatcc ggaaagcggc tactacgtca aaggcgaacg
      301 agaaaaacaa tttgcttatt ctgcccatac ttcatgcgac gacaatggat ttattcttag
      361 cacaatcatc actcctggaa atattcatga cagtcaagtc gcctttcagc tcgtcaaaca
      421 atcgaagaga ctttttcctg aaatcaattg tgttgtcgct gatgctgggt ataagacacc
      481 taagtttgtt cactttttga ctcatttgaa ccttcgacct tgtttgcctt attcaaggcc
      541 caaagggaaa aaggggctat tatcaaaaaa tgagtttctc tatgacgagt attttgattg
      601 ttatatttgt ccgcaagatc aaatgctcgc tttttcaacg gttactcgag aaaggtaccg
      661 agaatacaaa tccaatccaa aagaatgtgt gaactgtcct ctgctaaacc aatgtacca
//
LOCUS       DAMYXM010000207          710 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.217, whole genome shotgun sequence.
ACCESSION   DAMYXM010000207 DAMYXM010000000
VERSION     DAMYXM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.217"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            580..>710
                     /locus_tag="PNS21_002934"
     CDS             580..>710
                     /locus_tag="PNS21_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDI5160548.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTD"
BASE COUNT          267 a          106 c           92 g          245 t
ORIGIN      
        1 tgttttgttt gtaattccta attttgctcc acttttagat aatttttagg tctatttaac
       61 tcatcagaca ttctatgctc attttctcca aatttgtttc aactctttcg tacttaattt
      121 tgcgtatttg aaaattcttc ttttagagta cttttattac gatactagta tgatatatat
      181 ctatagcaaa cagttaaaat cttcctaaaa taagcgaata gcattcctaa aacctataaa
      241 atttttagat gaagcgacaa gtggacaatt aataaactac ttgaacatga aattatggat
      301 cacctttcaa atttagaaaa tggaattctt atacttattg cacaacattt attgattgca
      361 aaattatgtg acaaaaagaa ctagtagaag aaggaataat ggcttgataa taaagtacta
      421 cctattaaag gtagtacttt atttaattta gtataaatta acatactaac ccgtggtgct
      481 agttaatttt ttcctagagg aaaatgtcta aacatgttgg taaatcaaca aaaaaacgca
      541 tatagtagtc tcaaaactcc caagaaatga ggactacata tgcgttcatc aaaatatact
      601 gaacattata cagatacttt tataacattc aaggaaatta tgagaactgt ttctaacata
      661 taccacaatt gtgtaccaga taagattaaa aaccgaagaa atactgatca
//
LOCUS       DAMYXM010000208          689 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.218, whole genome shotgun sequence.
ACCESSION   DAMYXM010000208 DAMYXM010000000
VERSION     DAMYXM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.218"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            82..>689
                     /locus_tag="PNS21_002935"
     CDS             82..>689
                     /locus_tag="PNS21_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HDI5160549.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRK"
BASE COUNT          230 a          119 c          135 g          205 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaatt
//
LOCUS       DAMYXM010000209          641 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.219, whole genome shotgun sequence.
ACCESSION   DAMYXM010000209 DAMYXM010000000
VERSION     DAMYXM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.219"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..630
                     /locus_tag="PNS21_002936"
     CDS             <1..630
                     /locus_tag="PNS21_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HDI5160550.1"
                     /translation="VGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEYGKLLYQI
                     WKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAFLKR
                     LVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRN
                     KFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          223 a          115 c          125 g          178 t
ORIGIN      
        1 gtgggctact atcttcgtta taaccttagc tatcgtgaag ttcaagaaat cttatatgat
       61 cgtggcatta acgtttctca tacgacgatt tatcgttggg tgcaagaata tggcaaacta
      121 ctctatcaaa tttggaaaaa gaaaaataaa aaatcctttt attcatggaa aatggatgaa
      181 acgtacatca aaattaaagg aaaatggcat tatttgtatc gagccatcga tgcagatggt
      241 ttaaccttgg atatttggtt acgtaaaaaa cgggacacac aagcagccta tgcttttctt
      301 aagcggttag tgaagcagtt tgatgaaccg aaggttgtag tcacagataa agccccctct
      361 attacaagtg cctttaagaa actaaaagaa tacggctttt atcaagggac agaacatcgt
      421 accattaaat acctgaataa tttgattgaa caagaccatc gtccagtaaa gagacgcaat
      481 aaattctatc gaagtttacg cactgcctct accacgatta aaggcatgga agccattcga
      541 ggattatata agaaaacccg aaaagaaggc actctcttcg ggttttcggt ctgtactgaa
      601 atcaaggtat tattgggaat cccagcttaa atcatagata c
//
LOCUS       DAMYXM010000210          628 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.220, whole genome shotgun sequence.
ACCESSION   DAMYXM010000210 DAMYXM010000000
VERSION     DAMYXM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.220"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            108..500
                     /locus_tag="PNS21_002937"
     CDS             108..500
                     /locus_tag="PNS21_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5160551.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
BASE COUNT          246 a           47 c          111 g          224 t
ORIGIN      
        1 catgatgaat ggatttattc ttcaagaaaa tacatcaatt ttgataagta gaaatggtaa
       61 aaacattgta tagcatttta cacaggagtc tggacttgac tgagtttatg gaagaagttt
      121 taattgatga taatatggtt tttgatattg ataatttaaa aggatttctt aatgatacca
      181 gttcatttgg gtttatagct aaagaaaata ataaaattat aggatttgca tattgctata
      241 cacttttaag acctgatgga aaaacaatgt tttatttaca ctcaatagga atgttaccta
      301 actatcaaga caaaggttat ggttcaaaat tattatcttt tattaaggaa tattctaaag
      361 agattggttg ttctgaaatg tttttaataa ctgataaagg taatcctaga gcttgccatg
      421 tatatgaaaa attaggtggt aaaaatgatt ataaagatga aatagtatat gtatatgatt
      481 atgaaaaagg tgataaataa atgaatatag ttgaaaatga aatatgtata agaactttaa
      541 tagatgatga ttttcctttg atgttaaaat ggttaactga tgaaagagta ttagaatttt
      601 atggtggtag agataaaaaa tatacatt
//
LOCUS       DAMYXM010000211          616 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.221, whole genome shotgun sequence.
ACCESSION   DAMYXM010000211 DAMYXM010000000
VERSION     DAMYXM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.221"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>616
                     /locus_tag="PNS21_002938"
     CDS             <1..>616
                     /locus_tag="PNS21_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HDI5160552.1"
                     /translation="DMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNE
                     LRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGF
                     STSLKEAYPYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNA
                     MIYPYTNGKIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIK"
BASE COUNT          217 a          124 c          113 g          162 t
ORIGIN      
        1 agacatgaac gccgcctact tccagcttac caaacgtatt ctgccaaatg cgaaagtagt
       61 gattgatcgg tttcatatta tcaaacacat gaatcaagcc tttaatgagt tgcgtatccg
      121 tgaaatgaat gaacttcgta aagtaggaca gaaaagtcag gcagaaaaac tgaaaaagaa
      181 ctggcgcttt ttgctaaaaa atcgtgcaaa catcaaccat tatgaataca aaacatggaa
      241 aagttttcga gcaccaaaat acccatttct tacggaagcg atgatgattg atcgattgct
      301 tggcttttct acgtccctga aggaggcgta tccctatttt catgaattag ttgaagcctt
      361 tcgagacaaa gaccctgact tatttttctc cttattggca gaacttcccg aaacgttaga
      421 tgacggcttt cgggaaaagc tccaaaacct tctgacctat gaagaaggca tcaccaacgc
      481 gatgatctat ccttacacca atggaaaaat agaagcgaag aatacccata taaagacaat
      541 gaaacgagta tcctacggat ttaaatcatt tgagaacatg agaattagaa tctttttgat
      601 caatcaatta atcaaa
//
LOCUS       DAMYXM010000212          612 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.222, whole genome shotgun sequence.
ACCESSION   DAMYXM010000212 DAMYXM010000000
VERSION     DAMYXM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.222"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            1..558
                     /locus_tag="PNS21_002939"
                     /pseudo
     CDS             1..558
                     /locus_tag="PNS21_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          254 a           52 c          113 g          193 t
ORIGIN      
        1 acagagcctt gggaagatga agtttttaga gtaattattg aatataacaa tgttcctatt
       61 ggatatggac aaatatataa aatgtatgat gagttatata ctgattatca ttatccaaaa
      121 actgatgaga tagtctatgg tatggatcaa tttataggag agccaaatta ttggagtaaa
      181 ggaattggta caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct
      241 aatgcagtta ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa
      301 tctggtttta gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa
      361 gattgttatt taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa
      421 tatttaattg agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt
      481 ggttatgata gtgtggcata tttagttaat aatgaataca tttttaaaac aaaatttagt
      541 actaataaga aaaaaggttg agagtgtaaa atattttgtg taaatgaaaa aatccataca
      601 aaaaaggaag tc
//
LOCUS       DAMYXM010000213          577 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.223, whole genome shotgun sequence.
ACCESSION   DAMYXM010000213 DAMYXM010000000
VERSION     DAMYXM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.223"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            312..>577
                     /locus_tag="PNS21_002940"
     CDS             312..>577
                     /locus_tag="PNS21_002940"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDI5160553.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLST"
BASE COUNT          196 a          106 c           82 g          193 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttccac
//
LOCUS       DAMYXM010000214          562 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.225, whole genome shotgun sequence.
ACCESSION   DAMYXM010000214 DAMYXM010000000
VERSION     DAMYXM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.225"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(80..>562)
                     /locus_tag="PNS21_002941"
     CDS             complement(80..>562)
                     /locus_tag="PNS21_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160554.1"
                     /translation="NLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVV
                     LRYNLFSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVL
                     ENINEVSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVLRYAYIY
                     "
BASE COUNT          201 a           83 c           62 g          216 t
ORIGIN      
        1 aaatcaataa aatcacttct tttccctcct atatcttatg atatgatccg aattattaaa
       61 atactatcag aaattttatt taataaatgt aagcgtatct caaaacatca tgctctctat
      121 attctttaga tagtaccgct tctttaaaaa aatgtgaatc ttttaaatag gggattagtg
      181 attcatcttc tgttgaattt agcaaatgaa tcactagctt ctttacttct aaaattgaaa
      241 cttcgttaat attttctaat actgttgtca aaatagtcgt agtatatgca ggctcacata
      301 tgaggtaatt tactatactg taggataagt tttgaaatgg attaacaatt atagaaacca
      361 ttcctacaat tctctcttta tgtcttaaaa agtaaaattc actcgtaaac gaaaataaat
      421 tatatctaag gacaacttct aattctttac tatttccttg atgataaata tcttcatgaa
      481 aatacatact ttcaggatga tctgtgataa aattataaat ttcttcataa tgtgattcat
      541 ttagtaattc taattctaaa tt
//
LOCUS       DAMYXM010000215          546 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.226, whole genome shotgun sequence.
ACCESSION   DAMYXM010000215 DAMYXM010000000
VERSION     DAMYXM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.226"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(271..534)
                     /locus_tag="PNS21_002942"
     CDS             complement(271..534)
                     /locus_tag="PNS21_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HDI5160555.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          213 a           97 c           75 g          161 t
ORIGIN      
        1 actctcctga attttttcat gttgaaaaca tcactaatca aaggcaaatt ataatttaga
       61 aatagaatga aaacactaaa tgaaaataga acattagaaa aaatagaatc aaaaaaaccc
      121 aagactagtg ccgaaataag ggaaacaatc gaagctacaa aaaataatga tttcgttgaa
      181 caggaccaat ttatttgttt ccgatatttc tcaaaaagaa ctttctttgg gaaagttaaa
      241 ggcgactgta aagtcgcctt tagtcctttt ttaccaagct gctgcatatt tagctccata
      301 ttttttaatt aatgatttgg ctgtagcaac aataccagct gtaattaatc cgcctccaac
      361 aactgcagca acaatagaaa tgattgtagc aaccgtgcta ccagtattaa ttatgtcaat
      421 aactttttta gctgcataag aagaaattcc aatgttagca cttagcattg gtacaccaac
      481 aaagaatccc gcaaaaccta aaattggtaa aactaataat aaattctttt tcataataat
      541 tcctct
//
LOCUS       DAMYXM010000216          530 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.227, whole genome shotgun sequence.
ACCESSION   DAMYXM010000216 DAMYXM010000000
VERSION     DAMYXM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.227"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(238..>530)
                     /locus_tag="PNS21_002943"
     CDS             complement(238..>530)
                     /locus_tag="PNS21_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160556.1"
                     /translation="LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVE
                     LDVNNYRELYVFNSLDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKM"
BASE COUNT          177 a           82 c           56 g          215 t
ORIGIN      
        1 taattttttt gttatttaaa attaggagca gtgccaattg ttttaatttt aaaggctttt
       61 tttatatctt ctaaattaga agatatttgt atatcaatat ggaatatatt accttttttg
      121 tattttttta tgttatcttt agctaaaaca tcaaatacat gtccttgaaa ttgtatcgag
      181 gcagctggat ttagtttaaa ttgaggaaac tttaccacag ctgattcaaa acacttgtca
      241 cattttttta cctttgtaaa aatattcgat tgaatattct atttggcacg actcacttgg
      301 cagtatatgt atgtcaatat ttttcatctg tttcataggt atttcaaaat ctaaactatt
      361 aaaaacatat aattccctgt aattatttac atctaactct actaaattta tctttggaac
      421 aatagatcct ccacccattc caccataata aaaaaaatct tgttctctat caaaatagca
      481 catattaagc tcagtttgtt tatcatcctc aataatttta taaatcaaac
//
LOCUS       DAMYXM010000217          527 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.228, whole genome shotgun sequence.
ACCESSION   DAMYXM010000217 DAMYXM010000000
VERSION     DAMYXM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.228"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(60..281)
                     /locus_tag="PNS21_002944"
     CDS             complement(60..281)
                     /locus_tag="PNS21_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDI5160557.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
BASE COUNT          184 a           88 c          102 g          153 t
ORIGIN      
        1 tgttttcatt aagacgtatt acctaagagt tccatgactt tagtctggta gcgtgtcagc
       61 taaccaaaaa agagaaaaag aaaagtaaaa aattcgactt tcttttttct atagtatata
      121 aatttttgca tatagataaa gatcaagata ccgataacgg aagctagatt gtaatctatt
      181 ttagtgacag tcaggtatag ccaagtaact agaagatcag tggaacctgc accataaaag
      241 tttgagtttg tcggtccgtt gacagttaaa agaaaaatca cgttgaagtt attgatattt
      301 ccgattacgc cgcttcgatt tggtcacgat ccaaattttg gataatccct gtagaaatta
      361 gcatagtagc tgggatacca attcacatat tgacaaagat aacagagact tttacataaa
      421 acggatcaat caggaaagga attgctcgat ccagccccag ttttccaaca atgtgttaac
      481 tggcctagcg tcattcagca aattatgcat gatgaggagc gaaatga
//
LOCUS       DAMYXM010000218          517 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.229, whole genome shotgun sequence.
ACCESSION   DAMYXM010000218 DAMYXM010000000
VERSION     DAMYXM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.229"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
BASE COUNT          225 a           53 c           82 g          157 t
ORIGIN      
        1 tatatccaac aaagaataag gtacaaaata caagcaagtg agaaattcag ggatgtcatt
       61 ataaaaaaac tagcgaacaa ttaaatagaa taacatccgt taattaaatg taagtatctt
      121 ttagatgaac gaaaaaacaa taaaatgctg taatattcgt gtttaaaaat aataaataaa
      181 aatagggaat tttctattgc aattttataa agagattgct atattattat ttgttctaaa
      241 tgattccttt aaggtgtcac taaaaattat aaaaaatagt gttgacgcac ttttgatgtc
      301 atgttataat aacgaagtcg tttggatgag atagctaata aagcttgatt ttatcgaaaa
      361 aaacttcgaa aaagttattg acaaataaca ataaagatgg tatgataatc aagtcgctgc
      421 taatgagtgg caaagacgtt tagaaaaaat aaacaaagaa gttgttgaca ttctcttctg
      481 aatctgataa gattaaagag ttgttaaaac aaaaaga
//
LOCUS       DAMYXM010000219          508 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.230, whole genome shotgun sequence.
ACCESSION   DAMYXM010000219 DAMYXM010000000
VERSION     DAMYXM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.230"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
BASE COUNT          228 a           70 c           81 g          129 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactc
//
LOCUS       DAMYXM010000220          486 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.231, whole genome shotgun sequence.
ACCESSION   DAMYXM010000220 DAMYXM010000000
VERSION     DAMYXM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.231"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
BASE COUNT          161 a           96 c           57 g          172 t
ORIGIN      
        1 gctaaatcac tctacaataa cggcgaaagc acaaaaaata aaaaaattat tacgttatta
       61 aaaaaacaga tgaaagatca ggttttatta gatctcattc tttcatcact tgaagggatt
      121 taatgaacta ctttttcctc tttgtcgttt ttatatattc gtattccttc aaacagtagg
      181 atagcctctt tcaatgcttg atatttgaac tgtccgttac ttaaaaattt agatgtgccg
      241 ccctcttcct agaacatatt cttttttact ttttcattaa ttgacagacc aatcgaacga
      301 aactgtaaat ttcgatagga taatgattgg tcaaactcat tcattttcaa gacatctcga
      361 ttcatattca agataggaat ctttttatca acatcacttt attcgtattt tcctctacca
      421 taaaagcttc acgctccaca aaattgtttt atacactact attcagccca ttcaacgacc
      481 aatgaa
//
LOCUS       DAMYXM010000221          469 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.232, whole genome shotgun sequence.
ACCESSION   DAMYXM010000221 DAMYXM010000000
VERSION     DAMYXM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.232"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>469
                     /locus_tag="PNS21_002945"
     CDS             <1..>469
                     /locus_tag="PNS21_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HDI5160558.1"
                     /translation="AKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVL
                     ETLSEEANLPNILGDKGYISHKIHEKLALKGITISVPPRKNMDKSEKLDHSLLGKQRK
                     TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYS"
BASE COUNT          162 a           85 c           85 g          137 t
ORIGIN      
        1 gccaaagttg gctataactc aacaaaaaaa tcttattttt atggtcttaa aattcatatg
       61 atcgttacta agactggctt tccgattact tactcaatca cgaatccagg tgtccatgac
      121 gtgaaagtgt tagagacttt atcggaagaa gcgaatcttc ccaatatcct aggggacaaa
      181 ggatatatta gccataaaat ccatgaaaag cttgccctta agggcattac tatatccgtt
      241 ccgccacgta aaaacatgga taaatcagaa aaactagacc acagcttact tggaaaacaa
      301 agaaaaacag ttgagactgt tttttcttcc ttagaaaaat taggttgtca aaatttcaac
      361 tcacgttctg ttaaagggct agagagtaga tttgaaagca tattactggc ttacagtgtt
      421 ctattaagtc gagcacaacg acgttttgaa ggaactttga gatattctt
//
LOCUS       DAMYXM010000222          457 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.233, whole genome shotgun sequence.
ACCESSION   DAMYXM010000222 DAMYXM010000000
VERSION     DAMYXM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.233"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..419
                     /locus_tag="PNS21_002946"
     CDS             <1..419
                     /locus_tag="PNS21_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI5160559.1"
                     /translation="EEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQLYQ
                     DILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGPLEC
                     LNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          156 a           92 c           81 g          128 t
ORIGIN      
        1 aagaagaagc aaagttaaac tatgaaaaac gggtttggcg tgccagtttt aaagattatt
       61 tgactgaaag tgagattgcc gatcgactgt tagctgcttg tcccaactta cgccaaggct
      121 atcagttata ccaagacatt ctttatgcgg taaagaaaag agatcagtca ctatttgagg
      181 attgtttaac tagagacatt agcggtctgc cagaaaccta tacaaccact ttacgaacgt
      241 ttaaaaagtt tctgccacag atccaaaatg cgcttcacta cagctattca aacggaccat
      301 tggaatgctt aaacaaccac atcaaagtat taaaacgcaa tgcgtacggc tttcgcaact
      361 tttacaattt caaactacga attatgattc gtcatggcaa aacattttta accaaataaa
      421 cagaacagaa agaggttttc ttctttctgt tctgtcc
//
LOCUS       DAMYXM010000223          454 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.234, whole genome shotgun sequence.
ACCESSION   DAMYXM010000223 DAMYXM010000000
VERSION     DAMYXM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.234"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(10..216)
                     /locus_tag="PNS21_002947"
     CDS             complement(10..216)
                     /locus_tag="PNS21_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDI5160560.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
     gene            complement(349..>454)
                     /locus_tag="PNS21_002948"
     CDS             complement(349..>454)
                     /locus_tag="PNS21_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDI5160561.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT          131 a          100 c           60 g          163 t
ORIGIN      
        1 tctatcctcc tatttatctg atgaaatttt cttatacgtt cgatttgata cacctaaaat
       61 actccctaga aatgctccaa aacctgtaat aatcatcacc gttatatctg tgtaccgcca
      121 gttaacacct tttccgacaa gaccgacaaa agtcgctaaa gcggggataa ctactatagc
      181 gatccatttc aaaatatcaa aggttctatt gttcatacta tccacctccc ttcattggtt
      241 gggctacttt ctttttcaat cgcttgtttt cttctatcaa tatttctctt tctgaaagca
      301 aggcatcaat ttgcccgtcc agttgttcaa ttttatcttt cttaattgtc aaattaagtt
      361 agacagatca tcaatactta cgtaactcaa aaatacttcc aaaggtgttc gataatccag
      421 tgattttcta ggaatattat ttcgtttaga tgcg
//
LOCUS       DAMYXM010000224          445 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.235, whole genome shotgun sequence.
ACCESSION   DAMYXM010000224 DAMYXM010000000
VERSION     DAMYXM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.235"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>445
                     /locus_tag="PNS21_002949"
     CDS             <1..>445
                     /locus_tag="PNS21_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDI5160562.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNA"
BASE COUNT          183 a           53 c           63 g          146 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaa
//
LOCUS       DAMYXM010000225          430 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.237, whole genome shotgun sequence.
ACCESSION   DAMYXM010000225 DAMYXM010000000
VERSION     DAMYXM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.237"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>430
                     /locus_tag="PNS21_002950"
     CDS             <1..>430
                     /locus_tag="PNS21_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HDI5160563.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT          143 a           84 c           73 g          130 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc
//
LOCUS       DAMYXM010000226          426 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.238, whole genome shotgun sequence.
ACCESSION   DAMYXM010000226 DAMYXM010000000
VERSION     DAMYXM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.238"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            33..>426
                     /locus_tag="PNS21_002951"
     CDS             33..>426
                     /locus_tag="PNS21_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI5160564.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTP"
BASE COUNT          158 a           79 c           70 g          119 t
ORIGIN      
        1 gacaaccaat tcacagaaaa gaggacttcc ctatgaatga ttttactaca gaaattgtgc
       61 aaactctagt cactaaaggc gatttaaatg aattattccg ttcgcactta gaaaaagcga
      121 taaacacact cctacggact gaattaacgg cttttttaga ttacgaaaaa tatgatcgca
      181 ctggttttaa ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat
      241 atggtgaatt aacattggaa atacctagag atcgtaatgg tgagtttaaa caacaaactt
      301 taccagccta caaaagaaca aacgatacat tggaaaccac tattatccat ttattcgaaa
      361 aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt catcactata
      421 ctccac
//
LOCUS       DAMYXM010000227          405 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.240, whole genome shotgun sequence.
ACCESSION   DAMYXM010000227 DAMYXM010000000
VERSION     DAMYXM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.240"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>405
                     /locus_tag="PNS21_002952"
     CDS             <1..>405
                     /locus_tag="PNS21_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160565.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          167 a           60 c           92 g           86 t
ORIGIN      
        1 accagaagat aaacttccag tgttgaaagc aaaagatggt tatacagatg cgaaatggcc
       61 agaggaagca actcaaccaa ttacagcaga tgatacagaa tttgtatcaa gtgcaacaaa
      121 actggatgat aaatctgacg ctgacaaata taatcctgaa ggtcaaaaag tgactacaga
      181 attgaataag gaaccagacg catttgaggg aattaagaac aaagaagatt taccaaaaga
      241 tactaagtat acttggaaag aaaaagtaga tgttagcgca gctggaaata aaaaagggac
      301 tgttgtagtg acatatccag atggatcatc tgatgaagtt gaagtagatg ttacagtgac
      361 agacaatcgt tctgacgccg ataaatatga gccaacagta gaagg
//
LOCUS       DAMYXM010000228          362 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.241, whole genome shotgun sequence.
ACCESSION   DAMYXM010000228 DAMYXM010000000
VERSION     DAMYXM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.241"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..156
                     /locus_tag="PNS21_002953"
     CDS             <1..156
                     /locus_tag="PNS21_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDI5160566.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..>362
                     /locus_tag="PNS21_002954"
     CDS             175..>362
                     /locus_tag="PNS21_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI5160567.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDG"
BASE COUNT          151 a           31 c           68 g          112 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aa
//
LOCUS       DAMYXM010000229          361 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.242, whole genome shotgun sequence.
ACCESSION   DAMYXM010000229 DAMYXM010000000
VERSION     DAMYXM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.242"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..>361)
                     /locus_tag="PNS21_002955"
     CDS             complement(<1..>361)
                     /locus_tag="PNS21_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160568.1"
                     /translation="DKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRISQKIFYSGLAY
                     LPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWNPLTPTTSLGT
                     RLVVSTICVVPAVLMGL"
BASE COUNT          125 a           62 c           62 g          112 t
ORIGIN      
        1 ttcaatccca taagtacagc tggaacaaca caaatcgtag aaacaactaa acgtgtgcct
       61 aaacttgttg ttggggtcaa aggattccaa aaaccagttg aatcaaaatg gaataactgc
      121 ggcatttctt gtaactgaaa aatattcact ccttgtaaac ttcctggaat aatctcaaat
      181 cgatgaaatg cttctggtat tcttgtaaca acatagttta aaggaagata agctaagcca
      241 ctataaaaaa tcttttgaga aatacgtttg tcatgttttt ctggtagctc ttctcctgaa
      301 ggagtatagc gatagaaatg agaaagatac ctattctttt ctaaattgaa tcgtttgtct
      361 t
//
LOCUS       DAMYXM010000230          354 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.denovo.243, whole genome shotgun sequence.
ACCESSION   DAMYXM010000230 DAMYXM010000000
VERSION     DAMYXM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.denovo.243"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..>354)
                     /locus_tag="PNS21_002956"
     CDS             complement(<1..>354)
                     /locus_tag="PNS21_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160569.1"
                     /translation="DTLEAMQGLYEGKYLSYPRTDTPYITEGEYAYLLDHLDEYKHFL
                     KAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQLSPLQQAIYEQVLKTT
                     VAMFAEKYTYEETTIL"
BASE COUNT           88 a           77 c           68 g          121 t
ORIGIN      
        1 caagatcgtt gtttcttcat aggtatactt ttccgcaaac atggccaccg ttgtttttaa
       61 cacttgttca taaatagcct gctgcagtgg acttagttgt tcaaaagccg ctgctgtcat
      121 aaccgttttt gtaggaatga tcgcgtaatg ttcctggact ttcttattat tcacataccg
      181 actattcggt gtatgaatag gagtcggaat cgcttcggct tttaaaaaat gcttgtattc
      241 gtctaagtga tccaacagat acgcatattc cccttccgta atataagggg tatccgttcg
      301 cggatagctt agatattttc cttcatacaa cccttgcatg gcttccaaag tatc
//
LOCUS       DAMYXM010000231        37315 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.guided.020, whole genome shotgun sequence.
ACCESSION   DAMYXM010000231 DAMYXM010000000
VERSION     DAMYXM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.guided.020"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            8..250
                     /locus_tag="PNS21_002957"
     CDS             8..250
                     /locus_tag="PNS21_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5160570.1"
                     /translation="MGLSNEYIEGIFVSAEMQSQGIGKILLNYVKGKRNKLILNVYQK
                     NTRAISFYQREGFEIQYSGLDEATGEKDYVMAWQQK"
     gene            429..659
                     /locus_tag="PNS21_002958"
     CDS             429..659
                     /locus_tag="PNS21_002958"
                     /inference="COORDINATES: protein motif:HMM:NF025559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HDI5160571.1"
                     /translation="MQDDDSGFTYEISRCLYFDTCKEHGYAEFCKVFCNHDWYAYGVL
                     KHHSRFIRKSTIAEDGTVCHDTIEKVTEKHFL"
     gene            693..875
                     /locus_tag="PNS21_002959"
     CDS             693..875
                     /locus_tag="PNS21_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylan 1,4-beta-xylosidase"
                     /protein_id="HDI5160572.1"
                     /translation="MAKEERFEKIYSQGALTTTEIWVDKETGVNYLYHACGYSGGLTP
                     LLDRDGKPVISQAINR"
     gene            988..2499
                     /locus_tag="PNS21_002960"
     CDS             988..2499
                     /locus_tag="PNS21_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160573.1"
                     /translation="MRRSGWTKWLIVIPAMLGIVLVTYVRYTTDIWGFGAFICQLIAI
                     LELAYGLRIAMLAQNRKKSYRLTPEERHEYARYLYEKQYHRYPAVANQMLLVMARMSV
                     LLNNYERAAQELADIRIDKFNPAQLKLYYYLKVVTAMAAGDATGIQESQMCYAGIPDT
                     KCTYPSKAELAMWIEQRAAAQMAEALKKAVPDKKEHPIRISIITLVLAYSTFFYGLWY
                     GIKRDMGYEVRYRFAEISITVITISLAVLLIWGIVSLYRHNCQALSIQFGKNKVALII
                     VYGAAAIFALLIIGMSGLSVVFGMDGTETVTGCDSKYTYITVQEDYGGERRYRTNNPF
                     VMQSMDGLWWSSDTIDEEDNQEEDTTELPDLDTENSTDSEDIQSGNTIEDYETEGQHD
                     GLTIQNAMLAVYNYLQEQNTLQNMAFSYTANAKGEVYAVVSETQETKNGNTVNVRYCL
                     YDNGSKTDADGNSCEELVLEKVYPDGNYETELVDFYLVNADTMQVTDEQKNTW"
     gene            2822..3046
                     /locus_tag="PNS21_002961"
     CDS             2822..3046
                     /locus_tag="PNS21_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HDI5160574.1"
                     /translation="MSEKTEITFMQTRLIRLASEEWHLPVEQIIHLFKEADVLGYIEK
                     CYGIFHCEGDEAVLEDITEFLQRKGIEISA"
     gene            3039..3605
                     /locus_tag="PNS21_002962"
     CDS             3039..3605
                     /locus_tag="PNS21_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HDI5160575.1"
                     /translation="MLELKDGFVLYHGSYCEVKEPDLAKCAKRKDFGQGFYLTTSKEQ
                     AESFLRTSIAKAIATGTIEEGQKFGYISTFEFKLSENLETHIFENADVDWLHCIAAHR
                     KKKMFIEVEREMAKYDIIAGKIADDATNATLTAYLAGAFGTAGDKVADDFCIRQLLPN
                     KLKDQYCFKTEAAIECLKFVKGEKIWLK"
     gene            3593..3823
                     /locus_tag="PNS21_002963"
     CDS             3593..3823
                     /locus_tag="PNS21_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160576.1"
                     /translation="MAEVTIPKEKMNYTIDLLITMVTDEIAEETGKDRNEVLTDFLCS
                     KTGKALYDEKTKLWCTGPAYIAELYMEELKKS"
     gene            4024..4389
                     /locus_tag="PNS21_002964"
     CDS             4024..4389
                     /locus_tag="PNS21_002964"
                     /inference="COORDINATES: protein motif:HMM:NF024360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HDI5160577.1"
                     /translation="MSRAKNPKYESMSLEELKANMEKSRQELEAAIHHKNILEQRKKL
                     VERRERSHRLIVKGAEFEKVFPLSRDLEQEDVQDMMNQLQNSSYNKSIVRQAHIAALH
                     KDQQQIAEAVKKTEKGDDS"
     gene            4389..6116
                     /locus_tag="PNS21_002965"
     CDS             4389..6116
                     /locus_tag="PNS21_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HDI5160578.1"
                     /translation="MALYHFSVRNVSRGKGQMVVASAAYISGQRIFDSYYNKIHDYTS
                     KSGVIFTEILTPEYVPERLTDRETLWNEVEHVERNNKAQLAYSFDIALQNELTLEENI
                     RLAKEFCQEQFVARGMIVDLAVHEGKAENEEEPDNPHFHVLVPIRPFTEEGIWGNKQR
                     REYILDEDGNRVKDAKGKDMFNAVSTTGWNDPELLKEWRRAWTEKVNGKFRECHMASR
                     IDHRSYKEQGIDLIPTIHEGYEVRAMEKKGIKTVIGELNRAIRQFNQMFISLKESIQW
                     MKTAYEEMKAELDRRQNPTLLESLQDYYDKKTQGRPPLPNFYAEMKQRGKNLSNLQEF
                     AKSISYLQTHQIETMDDLQERIEELNGVVSVSKKEISEKRKQLKELENLEKMAEVIKT
                     NQPLIDEYNHFYFQKRRERYYQQHKKEINYYRKCERELKQHLDKNGKVPTARWKREKE
                     ELQAGIEELKADNQPYQEELTFVKKVQSCADIARRDREMAEADTSGRSEEKREKQVKF
                     PTFYTVQAKEILEENGKAEQQPLNQSEQNPEKKTSLLRKLDEKKKECAERDAKQQAVK
                     KKRNYDMSL"
     gene            6146..8062
                     /locus_tag="PNS21_002966"
     CDS             6146..8062
                     /locus_tag="PNS21_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI5160579.1"
                     /translation="MSKMQYTEEQIIRANQTDLVSFLSAQGEQLGKSGKEYRWKKHDS
                     VTVSGNRWYRHSQGRGGYPVDFVMEFYNATFPEAVKMLTGEEGEGRNSTCPAPSPDFR
                     LPEKEENNDRIIRYLTENRGIEKNMVEEWIGSGDIYEEKKHHNVVFVGRDADGIPRYA
                     HCRGTGETKYRGDVAESDKSYGFCHRGTDNQLFVFEAAIDLLSFIQLFPKDWKKRSYL
                     SLGGISSAALMAFLSERPQITSVFLCLDNDHAGNEASEKLAIEIPDGYSVIRLKPSRK
                     DWNEILCDKNADRKKSIIETVTMKVPEKEELVPMLCYEDIEQTSVEWLWFPYLPFGKL
                     TIIQGNPGEGKTYFAMMLTAACTNRKTFPNMEEIEPFNVIYQTAEDGMGDTIKPRLVE
                     AGADLSRVMVIDDTEEALTLSDDRIEKAIRQNQVRLLIIDPVQAFIGADVDMNRANEV
                     RPVFRKLGMIAEKTGCAIVLIGHLNKSSGTQSTYRGLGSIDIMAAVRSLLFIGKVKKD
                     PTTRVLIHEKSSLAPPGETMAFKLGDEEGFRWVGAYEISADDLLDGKEGKPTETKLQR
                     GTKLIYELLADGNAVTIRELDEKAKAQGISQRTMREARSRMKEELDYRMNEKQENTIR
                     LKKQGRMGDGRILE"
     gene            8747..8929
                     /locus_tag="PNS21_002967"
     CDS             8747..8929
                     /locus_tag="PNS21_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160580.1"
                     /translation="MAVKKKIINIRVYFDGDQDAKEVFAEVIAQKIRDTKTKDKLAIM
                     KDKQYNDYEFSNLKSA"
     gene            8956..10653
                     /locus_tag="PNS21_002968"
     CDS             8956..10653
                     /locus_tag="PNS21_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI5160581.1"
                     /translation="MNNRYMSNNIERNATWRVGVYCRLSKDDELTGESASISNQRDIL
                     VNYCQSQGWQVVDVFQDDGYTGLNTDRPDLQRLLKACEKGLVNLVITKDQSRLGRNHV
                     QTGFLMEEFFPKHGVRYIALYDNVDTFAGDNEIAPFKNVLNEMYSRDISKKVHASYHL
                     QATKGKFTGVVPPLGYQKDPEEKGHLLIDDETAPIVRKIFQWAVDGHGINFISRELER
                     QQIPCPSWWHRKRGLRTYYTKWEKKDPENGKYVWDESYLKSLLMNPVYCGDMASQKRY
                     YRFKIGNQGDKAPEDWITVRDTHEAIIPQDVFDLVQAKMKGRKRQTEQGEYSMFAGLL
                     RCAECGGALTLKKTHTKDQHEVYTCSTYIHKGKAHCTQHRVDADDLYDAVLTRIQECA
                     KAVTGDGTELENRVKELCEEDTQGHRESLEKLVSKQKDRLETLDRLIAKLYDDLINDK
                     ITESVFDKMLEKTQKEQTDIKKELFQNESVLNTEEKLDAQSQQWIDDISEYADIKELD
                     ANLLNRLISKIVISEPQEKDGTENYCRTPETVTMEIHFNLKPIPELGTIERGSGSHK"
     gene            10678..10809
                     /locus_tag="PNS21_002969"
     CDS             10678..10809
                     /locus_tag="PNS21_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160582.1"
                     /translation="MLIFSRLISSKYFLIKDIKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            10901..11764
                     /locus_tag="PNS21_002970"
     CDS             10901..11764
                     /locus_tag="PNS21_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160583.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            11801..12337
                     /locus_tag="PNS21_002971"
     CDS             11801..12337
                     /locus_tag="PNS21_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HDI5160584.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            12351..12773
                     /locus_tag="PNS21_002972"
     CDS             12351..12773
                     /locus_tag="PNS21_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HDI5160585.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            12703..15102
                     /locus_tag="PNS21_002973"
     CDS             12703..15102
                     /locus_tag="PNS21_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HDI5160586.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            15134..17125
                     /locus_tag="PNS21_002974"
     CDS             15134..17125
                     /locus_tag="PNS21_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDI5160587.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            17148..17399
                     /locus_tag="PNS21_002975"
     CDS             17148..17399
                     /locus_tag="PNS21_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HDI5160588.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            17389..18618
                     /locus_tag="PNS21_002976"
     CDS             17389..18618
                     /locus_tag="PNS21_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HDI5160589.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            18615..20696
                     /locus_tag="PNS21_002977"
     CDS             18615..20696
                     /locus_tag="PNS21_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HDI5160590.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            20845..24765
                     /locus_tag="PNS21_002978"
     CDS             20845..24765
                     /locus_tag="PNS21_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HDI5160591.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            24766..25710
                     /locus_tag="PNS21_002979"
     CDS             24766..25710
                     /locus_tag="PNS21_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160592.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            25714..25917
                     /locus_tag="PNS21_002980"
     CDS             25714..25917
                     /locus_tag="PNS21_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HDI5160593.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(26217..26453)
                     /locus_tag="PNS21_002981"
     CDS             complement(26217..26453)
                     /locus_tag="PNS21_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI5160594.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(26636..27022)
                     /locus_tag="PNS21_002982"
     CDS             complement(26636..27022)
                     /locus_tag="PNS21_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HDI5160595.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(27004..28332)
                     /locus_tag="PNS21_002983"
     CDS             complement(27004..28332)
                     /locus_tag="PNS21_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDI5160596.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(28293..28622)
                     /gene="mobC"
                     /locus_tag="PNS21_002984"
     CDS             complement(28293..28622)
                     /gene="mobC"
                     /locus_tag="PNS21_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HDI5160597.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(28880..29251)
                     /locus_tag="PNS21_002985"
     CDS             complement(28880..29251)
                     /locus_tag="PNS21_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI5160598.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            29396..29581
                     /locus_tag="PNS21_002986"
     CDS             29396..29581
                     /locus_tag="PNS21_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI5160599.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            29739..29906
                     /locus_tag="PNS21_002987"
     CDS             29739..29906
                     /locus_tag="PNS21_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160600.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            30524..31183
                     /gene="vanR"
                     /locus_tag="PNS21_002988"
     CDS             30524..31183
                     /gene="vanR"
                     /locus_tag="PNS21_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HDI5160601.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            31186..32529
                     /gene="vanS"
                     /locus_tag="PNS21_002989"
     CDS             31186..32529
                     /gene="vanS"
                     /locus_tag="PNS21_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDI5160602.1"
                     /translation="MERKGIFIKVFSYTIIVLLLVVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQNKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            32700..33506
                     /locus_tag="PNS21_002990"
     CDS             32700..33506
                     /locus_tag="PNS21_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HDI5160603.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            33524..34351
                     /gene="vanW"
                     /locus_tag="PNS21_002991"
     CDS             33524..34351
                     /gene="vanW"
                     /locus_tag="PNS21_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HDI5160604.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            34348..35319
                     /locus_tag="PNS21_002992"
     CDS             34348..35319
                     /locus_tag="PNS21_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HDI5160605.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            35312..36340
                     /gene="vanB"
                     /locus_tag="PNS21_002993"
     CDS             35312..36340
                     /gene="vanB"
                     /locus_tag="PNS21_002993"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HDI5160606.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            36346..36954
                     /locus_tag="PNS21_002994"
     CDS             36346..36954
                     /locus_tag="PNS21_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HDI5160607.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(37020..37193)
                     /locus_tag="PNS21_002995"
     CDS             complement(37020..37193)
                     /locus_tag="PNS21_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160608.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT        10225 a         8696 c        10016 g         8378 t
ORIGIN      
        1 gggttttata gggttaagta acgaatacat tgaaggtatt tttgtttctg ccgaaatgca
       61 atctcaaggt ataggtaaaa ttttgctaaa ctatgtaaag ggaaaaagaa acaaattaat
      121 cttaaatgta tatcaaaaaa atacacgggc aatatccttt tatcaaagag aaggatttga
      181 gattcagtat agcggtttgg atgaagctac tggagaaaaa gactatgtaa tggcatggca
      241 gcagaaatag aaattcaagt tttagtgttt ggaaaattat gacttagtgt gcaagttggg
      301 agaaaatatg gtaacggaaa aagagaaaaa agcatgtcga tttattgccg aatggctata
      361 agattgtgag acagtctctg gtagatgatc tggagggcga aaaaaaggta tgttgggaga
      421 cagaaatttt gcaggatgat gatagtggat ttacttatga aatcagcagg tgtctgtatt
      481 tcgatacctg taaggaacat ggctatgcag agttctgcaa agtattttgc aaccatgact
      541 ggtatgctta tggagtatta aagcatcatt cgagatttat cagaaaatcg acgattgccg
      601 aagatggaac tgtatgtcat gatacaatcg agaaagtaac agagaaacat tttttataaa
      661 accgaaaaat tgattatggg aggaaattga aaatggcgaa agaagagaga tttgaaaaaa
      721 tatattctca gggagcatta actactactg agatatgggt ggataaggaa actggtgtga
      781 attatttata tcatgcatgt gggtattcag gtggactgac tccattgttg gacagagatg
      841 ggaaaccagt tatttctcag gctataaata gatgacctta gaaacagcaa ctgttatggt
      901 aagtaacagc atcgtcacaa tatgtatatg gtcgattggt gtgtgcggta atatactata
      961 ttcagaaaaa caattcagga gatttttatg agaagatcag gttggacaaa atggttgatc
     1021 gtgattccgg caatgctagg cattgtgttg gtgacctatg tgcggtatac aacggatatc
     1081 tggggatttg gtgcatttat ctgccagctg attgcaattt tggaattggc ttatggtctg
     1141 cgaattgcga tgctggcaca gaatagaaag aagtcctatc ggttaacacc ggaagaacga
     1201 catgagtatg cacggtattt atatgagaag caatatcacc gttaccctgc tgtagcgaat
     1261 cagatgcttt tggttatggc acggatgagt gtactcctta acaattatga acgggctgca
     1321 caggagctgg cagatattcg tatagataaa ttcaatccag cacagttgaa gttgtattat
     1381 tacctgaaag tggttacagc aatggcagca ggagatgcga cagggattca agagagccag
     1441 atgtgttacg cagggattcc ggatactaaa tgcacatatc cgtcaaaagc agagcttgct
     1501 atgtggatag agcagagagc ggcagcgcag atggcagagg cactgaagaa ggcagtgccg
     1561 gataaaaaag agcatccgat acggatcagt atcattactt tggttcttgc gtatagtacc
     1621 ttcttctatg gattgtggta tggtataaaa cgtgatatgg gatatgaagt gagatatcgt
     1681 ttcgcagaaa tatcgattac tgttattaca atcagtctgg cggttctttt gatctggggg
     1741 atcgtgagtt tatacagaca caattgccag gcattatcca tacaattcgg taaaaacaaa
     1801 gtggcattga taattgtcta tggagctgct gcgatatttg cacttttgat tattggaatg
     1861 agtggtctga gtgtagtgtt cggaatggat ggaacagaaa cggtgactgg ttgtgacagc
     1921 aaatatacgt atataactgt gcaggaagat tatggtggtg aacggagata cagaaccaat
     1981 aatccatttg ttatgcagag tatggatggt ctgtggtggt cttcggatac aatagatgaa
     2041 gaagataatc aagaagagga tactacagaa ttaccggatt tggatacaga gaattcaaca
     2101 gattcagagg atattcagag tggaaatacc atagaagatt acgagacaga agggcagcat
     2161 gatggtctga caattcaaaa tgcaatgctg gcagtttata attaccttca ggagcagaat
     2221 acacttcaaa atatggcgtt cagttacacg gcgaatgcaa agggagaagt ctatgctgtc
     2281 gtcagcgaaa cgcaggaaac aaagaacggt aatacggtaa atgtgagata ctgtctgtac
     2341 gataatggtt ccaagacaga tgcagatggt aattcctgcg aagaactggt gttagaaaaa
     2401 gtatatccgg atggcaatta cgagaccgaa ttggtagatt tttatctggt aaatgcagat
     2461 acgatgcagg tgacagacga acagaagaat acgtggtaaa ataaattctg gggaagtagg
     2521 tgcattgtat gacaatgaga gaaaaaataa aactattatc gtaaatgttt caagcggact
     2581 gggttctttt ggaagtatgc aaaaggactc caagacataa gaggtgaact gcgctgatcc
     2641 aggtgccacg gcaacagatt tgaatgtcga cggaggaaag caaacagtac aggcgcaatc
     2701 gtaaaacttg caatgattga taaaaccgga ctaacagtaa cttttattag tcgatttgga
     2761 aatatgcctt ggtaatcaac aattccggtt ttactgacta agaaaatgag gaggattcat
     2821 aatgagtgag aaaacagaga ttacttttat gcaaacaaga ctaatccggc ttgcttcaga
     2881 agagtggcat ttacctgttg aacagattat ccacttgttt aaagaagccg atgttcttgg
     2941 atatatagaa aaatgctatg ggatttttca ttgtgaaggc gatgaagctg tgcttgaaga
     3001 tattactgaa tttttgcaaa gaaaggggat agagattagt gcttgagtta aaagatggat
     3061 ttgttctcta tcatggaagt tattgtgaag taaaagagcc agatcttgcc aaatgtgcaa
     3121 agagaaaaga ttttggacag ggattttatt taaccacatc aaaggaacag gcagaaagtt
     3181 ttctgagaac ctcaatagcc aaagccatcg caaccggaac aattgaagag gggcagaagt
     3241 ttggatatat ttcaactttt gaattcaaac tttcagaaaa tcttgaaacg catatttttg
     3301 aaaatgcaga tgtggactgg ttacattgta tcgccgctca ccgaaagaaa aaaatgttta
     3361 ttgaggttga acgtgaaatg gcaaaatatg atatcattgc aggaaagatt gcggatgatg
     3421 caacaaacgc tactcttacc gcttatcttg cgggtgcttt tgggactgca ggtgataaag
     3481 tagcagacga tttctgtatc agacagttgc tgccgaataa attgaaagat caatactgct
     3541 ttaaaactga ggcagctatt gagtgtctga aatttgtgaa aggtgagaag atatggctga
     3601 agtaacaatt ccaaaggaaa aaatgaatta tacaatcgac cttctgatca ctatggtgac
     3661 agatgaaatc gcagaagaga caggcaagga cagaaacgag gtattgacag attttttgtg
     3721 ttcaaagaca ggaaaagcac tgtatgatga gaagacaaag ctgtggtgca ctggtccggc
     3781 atatatagct gaactgtata tggaagaatt aaagaaatcc tgatatggga tgtgagttta
     3841 aatttatacg acaatggtga caaagacttt cattccaagg agcaacaatt aaatatttca
     3901 aaaacgctga ggtcagcatt tcctaatcac aggacaacga cctcagtttt ttatacccag
     3961 gaatcggcat tcatgaataa gccggttctt tttgtttaca gaaaaaagaa ggagtacaag
     4021 aaaatgagca gagcaaaaaa tccaaagtat gaatccatgt cattggagga actgaaggcc
     4081 aacatggaaa agagcagaca ggaactggag gcagcaattc accacaaaaa cattctggag
     4141 cagcggaaga agctggtaga acgcagggaa cgttcccacc ggctgattgt caaaggggca
     4201 gagtttgaga aagtatttcc gctgtccaga gatctggaac aggaagacgt ccaggacatg
     4261 atgaatcaac tgcagaacag cagttacaac aagagtattg tgcggcaggc tcatatagcc
     4321 gccttacaca aagatcaaca gcagattgca gaagcagtaa aaaagacaga gaagggagat
     4381 gacagttaat ggcactgtat catttttcag taaggaatgt atccagaggg aaagggcaga
     4441 tggtggtcgc ttccgcagcc tatatttccg ggcagaggat ttttgacagt tattacaaca
     4501 agatacacga ttatacgagc aagtcaggtg tgatatttac agaaatcctg acaccggagt
     4561 atgtgccaga gcgtctgact gacagggaaa cactctggaa cgaggtggag catgtggaac
     4621 ggaacaacaa ggcacagctt gcctattcgt ttgacattgc actgcagaat gaattgacac
     4681 ttgaggaaaa tatcagactg gcaaaagagt tctgccagga gcagtttgtg gcacgtggca
     4741 tgatcgtgga tctggcagtc catgagggaa aagcggaaaa tgaagaagaa ccggataatc
     4801 cccatttcca tgtgcttgtc ccaatccgtc cttttacaga ggaaggaatc tggggcaata
     4861 agcagagaag ggaatatatt cttgatgagg atggaaaccg ggtgaaggat gccaaaggaa
     4921 aagatatgtt caatgctgtt tctactaccg gatggaatga tccggagctt ttaaaagaat
     4981 ggcggcgggc atggacagag aaagtaaatg ggaaattcag ggagtgccac atggcatcca
     5041 gaatcgacca ccgttcctac aaagaacagg ggattgacct gatcccgacc atccatgagg
     5101 gatatgaagt ccgggcaatg gagaaaaagg gaatcaaaac ggtgatcgga gaattgaacc
     5161 gggcaatccg gcagtttaac cagatgttta tttctctgaa agaatccatt cagtggatga
     5221 agacagctta tgaggaaatg aaagcagagc tggatcgcag gcagaacccg acactgctgg
     5281 aaagcctgca ggattattat gataaaaaga cacagggcag accaccactt cccaattttt
     5341 atgcggagat gaaacagaga ggtaagaatc tctccaattt acaggagttt gcaaaatcca
     5401 tcagttatct tcagacacat cagatagaaa cgatggatga cttgcaggaa cggattgagg
     5461 aactgaacgg tgttgtttct gtcagtaaaa aagaaatttc tgaaaagcgg aaacagttaa
     5521 aggaacttga gaatctggaa aagatggcag aggtaataaa aacaaatcag ccattgattg
     5581 atgagtataa ccatttctat tttcagaaaa gacgggagag atattatcag cagcataaaa
     5641 aagaaatcaa ttattaccgc aaatgtgaac gggaactgaa acaacatctg gataaaaatg
     5701 gaaaagtgcc aactgccaga tggaaacggg agaaagaaga acttcaggcg gggatcgaag
     5761 agctgaaagc agataatcag ccgtaccagg aggaactaac ttttgtgaaa aaggtacaga
     5821 gctgtgcgga tattgccaga cgtgaccggg aaatggcaga agcagatact tccgggcgtt
     5881 cagaagaaaa aagggagaag caggtgaagt tcccgacatt ttacacagtt caggcgaaag
     5941 agattcttga agaaaacggg aaagcagagc aacagccgtt gaaccagtcg gaacagaatc
     6001 cagaaaagaa gacctctctt ctgagaaaac tggatgaaaa aaagaaagag tgtgcagagc
     6061 gtgatgcaaa acaacaggca gtgaagaaaa agcgaaacta tgacatgagt ttataacagt
     6121 ctgtcagcag gacagacggg aaaggatgag caaaatgcag tatacagaag aacaaattat
     6181 aagagcaaat cagacagatc ttgtttcttt tctgagcgca cagggagaac aactagggaa
     6241 aagcggaaag gaataccgtt ggaaaaagca tgacagtgta acggtatccg gaaaccggtg
     6301 gtacaggcac agtcagggga ggggtggcta tccggtggat tttgtgatgg aattttacaa
     6361 tgccacattt ccggaagctg tcaagatgct aaccggggag gaaggcgaag gcaggaacag
     6421 tacctgcccc gccccgtccc ctgacttccg attaccagag aaagaggaaa ataatgatag
     6481 aatcatcaga tatctgacag aaaacagagg gattgaaaag aatatggtgg aggaatggat
     6541 cggcagtggg gatatttatg aagaaaagaa gcaccataat gtggtgtttg ttggcagaga
     6601 tgcagacggt attccaagat atgcccattg caggggaact ggtgaaacaa aataccgggg
     6661 agatgtggct gaatctgata aatcttacgg attctgtcac agagggacgg ataatcagtt
     6721 gtttgtgttt gaagcagcca ttgatctgct ttcttttatc caattatttc ccaaagactg
     6781 gaaaaagcgg agctacctga gtctgggtgg catttccagt gcggcactga tggcttttct
     6841 ttctgaacgc cctcagatta cttcggtttt cctgtgcctg gataatgacc atgcagggaa
     6901 tgaagccagt gagaaattgg caatagaaat cccggacgga tacagtgtca tccgtctgaa
     6961 accgtcccga aaggactgga atgaaatcct ctgtgataaa aatgcagaca ggaagaaatc
     7021 aatcattgaa acagttacaa tgaaagtacc ggagaaggaa gaactggtgc cgatgttgtg
     7081 ttatgaagac attgaacaga ccagtgtaga atggctgtgg tttccctatc tgccatttgg
     7141 aaaactgacg attattcagg gcaatccagg agagggaaaa acttattttg ccatgatgct
     7201 gaccgcagcc tgcacgaacc gtaagacttt cccgaacatg gaagagattg aaccttttaa
     7261 tgtgatctat cagactgcag aagacggtat gggagatacg attaagccga ggctggtgga
     7321 agcaggagca gacctgagtc gggtgatggt cattgatgat acagaggaag cattgacgtt
     7381 atccgatgac cgtattgaaa aagcaatccg tcagaatcag gtcagacttt taatcattga
     7441 tccggtacag gcttttatcg gtgccgatgt ggatatgaac cgggcaaatg aagtccgtcc
     7501 ggtattccgg aaactgggaa tgatcgctga aaagaccggt tgtgcgattg tgctgatcgg
     7561 acatctgaat aaatcttcag ggacacagag tacttaccgg ggacttggtt ctattgatat
     7621 catggcggcg gtgagaagcc tgctttttat cgggaaagta aagaaagacc cgacaacaag
     7681 agtactgatc catgagaaaa gttctcttgc acctccaggt gagacgatgg catttaaact
     7741 tggagatgaa gaaggattcc gctgggtggg agcttatgag atttccgcag atgatcttct
     7801 ggacggaaag gaaggaaagc cgacagaaac aaaactgcaa cggggaacga aactgatcta
     7861 tgaactgctt gcagacggaa atgcagtcac aatccgggaa ctggatgaaa aggcaaaagc
     7921 acagggaatc tcccagagaa ccatgcgaga agccagaagc cggatgaaag aagaactgga
     7981 ttatcggatg aatgaaaagc aggaaaatac cattcgatta aaaaaacaag ggagaatggg
     8041 tgacggcaga attttggagt gaacggcagg ctttatagat accctatctg ccgtctgccg
     8101 tctgccggtg caaggcaatc aaacggaaat agcaggtatc catacagaat aaggattgct
     8161 gtgaatctgg aacaaaaatg aattgtaata tggcggatat aatggtatga ttatccggta
     8221 acagcaatgt tacggggaag cacctgtgac agggtgtggc tgcctcctga tatttacaga
     8281 ccggagcctt tcggggttct gagatgaaag aaaggaggtg cgtgatatga cagcttatga
     8341 gattatctca atattcttag gcgtactcag tttactgatc gcttttggta gcctgttaat
     8401 tgcgattatt gcctttcttg atcggaagaa caagaggaaa taaaaatgcc tatcctgtct
     8461 gccaacagga taggcggtgg tcgtaagacc atttagtcaa ctcaggcggc atccactttg
     8521 gagtgggtgc ttttcctata acaaatgtac cacacattcc tcttgttttc aatgggaaaa
     8581 ttaaaatatg cagttcatct gacagggatt tatccctgtc tttattatat ccccaatttc
     8641 tgaaatgtta atacaaaaat tttaatatat gtaaccaaca gtctgggcag ttaatctgta
     8701 aaaatggatt agctgctctt tttttgcaaa aaatggaggt tttacaatgg cagtgaagaa
     8761 gaaaattatc aacatcagag tatattttga cggtgatcag gacgcaaaag aagtgtttgc
     8821 agaggtgatc gcacagaaaa tacgtgacac gaaaacaaaa gataaacttg caataatgaa
     8881 agataagcag tataatgatt atgagttcag taatctgaaa tctgcgtagg atgtgcaaag
     8941 taacggaggt atcagatgaa caacagatat atgagtaaca atatagaaag aaatgcaacc
     9001 tggagagttg gtgtatactg ccgactttcc aaagatgacg aattgaccgg agagagtgcc
     9061 agcatctcca accagagaga catactggtg aattactgtc agtcacaggg ctggcaggtg
     9121 gtggatgtgt ttcaggacga tggttataca ggattgaaca cagaccgacc tgatttgcag
     9181 agacttctca aagcctgtga aaaaggactg gtgaatcttg taattaccaa agaccagagc
     9241 cgactgggac gaaaccatgt acagacagga tttctgatgg aggagttttt cccaaagcat
     9301 ggagtaaggt atatcgcatt atatgataat gtggatacct ttgccggaga caatgagatt
     9361 gccccattta agaacgtgct gaacgagatg tattccagag atatttccaa gaaagtccat
     9421 gcgtcctatc acctgcaggc aaccaaggga aagtttacag gtgttgttcc accactggga
     9481 tatcagaaag acccggagga aaagggacat ctcctgattg acgacgagac agcaccgatt
     9541 gtccggaaaa ttttccagtg ggcggtggac ggtcatggga ttaactttat atccagagaa
     9601 ctggaacgcc agcagattcc ctgcccgtca tggtggcacc ggaaacgggg actccgcacc
     9661 tattacacaa aatgggaaaa gaaagacccg gaaaatggga aatatgtatg ggatgaatcc
     9721 tatctgaaat ccctgctgat gaatcccgtt tactgtggtg acatggcttc ccagaaaagg
     9781 tactaccgtt tcaagattgg caatcaggga gataaagctc cggaggactg gatcacagtc
     9841 agggataccc acgaagcaat cattccgcag gatgtatttg atctggtaca ggcaaagatg
     9901 aaaggcagga aacgccagac agaacaggga gaatacagca tgtttgccgg attactccgc
     9961 tgtgcagagt gtggcggtgc gttgacgttg aagaagacgc acaccaaaga ccagcatgaa
    10021 gtttatacct gttccactta tatccataag gggaaagcac attgtaccca gcaccgggtg
    10081 gacgctgatg acctgtatga tgcagtcctt accagaatcc aggaatgtgc aaaagcagtt
    10141 accggagacg gaacagaact ggaaaaccgg gtaaaggagt tatgcgagga agatacacag
    10201 ggacacaggg aaagtctgga gaagctggta tcaaagcaga aagaccgcct ggaaacgtta
    10261 gaccggctga ttgcaaaact gtatgatgac cttatcaatg acaagattac agaatctgtt
    10321 tttgataaga tgctggagaa gacacagaag gagcagacag atatcaaaaa ggaacttttc
    10381 cagaatgaga gtgtgctgaa tacggaagaa aaactggatg cacagtccca gcagtggatt
    10441 gacgatatta gcgagtatgc ggacatcaaa gaactggatg ccaatcttct gaaccggctg
    10501 atcagtaaaa tcgttatctc agaaccgcag gagaaagacg gaacagaaaa ttactgccgt
    10561 actcctgaaa cggtcacaat ggaaattcat ttcaacttaa aaccaatacc ggagcttgga
    10621 accatcgaaa gaggttctgg ctcccataaa taagaaaaat ctatcaaaat ttaatctatt
    10681 ttaatctttt caaggctcat ttcgagcaaa tattttctca taaaagacat aaaacagctt
    10741 gtcgctggcg gcggcgtggc cctgatcggt gtcactctgg ttcccctgct gtccgggctg
    10801 ctgggctgat cctggccttt gtccctgtcc aaacccttaa ctaatcgcct ccgcgtcctc
    10861 ccggccagac cgggagggcg cgggaaagga ggtgctaccc gtgattagtg aaatcattga
    10921 agaatggata aaaggcatcc tcatggacgg tatcacgggc aacctgtccg ggctgttcga
    10981 tacggtaaat gccaaagtcg gagaaatcgc cgccgacgtg ggacgaaccc cgcaggcatg
    11041 gaacagcggt attttcaata tgctgcacag cctgtcggaa accgtgatcg tccccatagc
    11101 cggggccata ctcgcccttg tcatgtgcta tgagcttatc cagatgataa cggaaaagaa
    11161 caatatgcac gactttgaca gctccatgtt cttccgctgg atattcaaat ccgcgtttgc
    11221 aatcctgatc gtaacgaaca catggaacat cgtcatgggc gtattcgacg ccacccaaag
    11281 cgtcgtcaat caaagcgccg gtgtgattat cgggcagaca tccattgatt ttaacaccct
    11341 gataccagac cttgaaagcc aattggaggc tatgagcgtg ggcgctcttt tggggctgtg
    11401 gttccaaacc cttgttgtgg gcctcaccat gaatatcctt tccatctgca ttttccttgt
    11461 gacctatggc cgcatggtgg aaatctatgt tgtcacgtcg ctggggccta tcccccttgc
    11521 cacaatgggc aacgcggaat ggcgcggcat gggccaaggg tatctgaaat ccctgctggc
    11581 gctgggcttc caagcctttt taatcatggt tgtggtgggt atctacgcgg tgctgataca
    11641 gaatatcggt tccgcttccg acatatccgg cgcaatctgg ggctgtatgg gctatacggt
    11701 gctgctgtgc ttctgcctgt tcaagaccgg cagtatatca aaggccgtat tcggggcgca
    11761 ttgatcccaa aatccaagca tacacaggag gaacttgttt atttcaacgt acaacatcat
    11821 ttacgctgac ccgccgtggc ggtatgactg caaaaacgta cagggcgcag cggaaaatca
    11881 ctattccacc atgagcattg acgagctgtg cgccctgccg gtggaacggc tggcgtccaa
    11941 agactgcctg ctgtttttgt gggccacatt ccccatgctg ccggaagctc tgcggttaat
    12001 caaagcgtgg ggcttttcat tcaagaccgt ggcttttgta tggctgaaac agaaccgtaa
    12061 aagccttaca tggttttacg ggctgggccg ctggacaagg ggaaacgcgg aaatctgcct
    12121 gctggcaaca cacggcaagc cgaagcgtcg ttctgcctct gtccatcagt ttattatcag
    12181 cccgattgaa caacacagca aaaagccgga tgtgacccgt gaaaaaatcg tagagctggc
    12241 gggcgatttg ccccgcgtgg agctgtttgc cagacagaaa acccccggct gggacgtgtg
    12301 ggggaacgag gtggaatgtg accttatcct gacgtgaaag gaggtgccaa atggcttatg
    12361 taaccgtccc caaggactta acccatgtga aaagcaaggt tgtgtttggg cttaccaaac
    12421 ggcaactgat ctgtttttcc ggggcgctgg tgatcggcgt ccccttatat tttctcaccc
    12481 gtgactatct taccaacagc gcggcggccc tgctcatggt atttgccatg ctgccgggct
    12541 ttttgcttgc catgtacgag cgtcacggcc agccccttga agtggtgatt atgcagatca
    12601 tccagtgctg cttcatccag ccaaaggagc gtccctatca gaccgacaac acctacaccg
    12661 cccttgtgcg gcaataccag atggaacagg aggtaaaggc cattgtccaa aaaggaaatc
    12721 ccaagagaaa ccgaaaaaac aaagctgacc cgcgcccagc gaaaagaaat tgacgcggta
    12781 atccgtaaat ataagggcga cggcaggccc cacacggcgc agcagagcat cccctacgag
    12841 gtcatgtacc cggacggcgt gtgccgggta tcgcccggcg tcttttccaa gtgcattgag
    12901 tttgcggaca tcagctatca gcttgcccag ccggacacgc agacggctat ctttgaaaaa
    12961 ctgtgcgacc tgtataacta tgtggacgcc tccattcata tccagttttc tttcctcaat
    13021 cggaaagttg acccggtgca gtacgccaag agctttgaga tcgcgccgca gggggacgac
    13081 ttcgacgaca tccgggccga gtacaccggc attctgcaaa agcagcttgc aaacggcaac
    13141 aacggcatgg taaagaccaa atacctgaca ttcaccatcg aggccgaaag cgtgaaagcg
    13201 gcgcgagcca gattgaagcg catcggcttt gacctgttag gctatttcaa gagcatgggg
    13261 gccgtagccc atgtgatgga cggctgggaa cggctgaacc tgctgcatgg cgtgtaccac
    13321 ccggacggcg agatattcaa ttttgactgg aagtggctgg ccccgtccgg cctctccacc
    13381 aaggatttta ttgccccgtc ctccctgtgc ttcggcaatg caaagacatt cggcatgggc
    13441 ggcaaatacg gcgcggtaag tttcctgcaa atcctgtccc cggagctgtc ggacgatatg
    13501 ctggccgact tcctcaatac ggaaagcggt gttttagtca atctccatgt gcaggccatt
    13561 gaacagacca aggcgatcaa gaccatcaag cgcaaaatta ccgaccttga cgctatgaag
    13621 attgcggagc agaaaaaggc cgtccgcagc ggttacgata tggacattct tcccagcgac
    13681 cttgccacct acggcgagga cgccaagaag ctgctgacaa agttgcagac ccgcaatgaa
    13741 cggctgttcc agttgacgtt ccttgtcctc aacgtggccg acaccaagca aaagctgaat
    13801 aacgacgtgt tccaagccgc aggcgtggcc caaaagcata actgtcccct tgtccggctg
    13861 gactaccagc aggaacaggg ccttgcaagc agcctgccgc tgggcgtcaa tcagatcaag
    13921 atacagcgca gccttacgac ttccagcgtg gccgtgttcg tcccctttgt gacgcaggag
    13981 ctttttcagg gcggcgcggc catgtattac ggcatcaacg ccaagtcccg caacatgatt
    14041 atgctggaca ggaagcaggc ccgttgcccc aatgccttga agctcggcac ccccggcagc
    14101 ggcaaatcca tgagctgtaa atccgagatc gtcagtgtgt tcttaacgac gcctgacgat
    14161 atttttattt cagacccgga ggccgaatat tacccccttg tcaaacggct ccacggacag
    14221 gtaatccggc tgtcgcctac cagcaaggac tttgtgaacc cgctggacat caacctgaat
    14281 tattcggagg acgataaccc gctggccctg aaatccgact ttgtgctgtc gttctgtgag
    14341 cttgtcatgg gcggcaagaa cgggctggaa gccattgaaa agaccgtgat agaccgggct
    14401 gtgcgcgtga tctaccggcc ctatctggca gacccccgcc cggagaatat gccgatcctg
    14461 tccgacctcc acaaagcgtt gttagatcag catgtcccgg aggctgaccg ggtggcgcag
    14521 gcccttgacc tgtatgtgtc cggctccctg aacgtattta accaccgtac caacgtggac
    14581 atcggcaacc ggcttgtgtc ctttgacatt aaggagctgg gcaagcagct caaaaagctg
    14641 gggatgctca ttgtccaaga ccagatatgg gggcgcgtca cggccaaccg aagccaagga
    14701 aaggccacat ggtattttgc cgacgagttc catttgcttt tgaaagagga acagacggcg
    14761 gcctattctg cggagatttg gaagcggttt aggaaatggg gcggcattcc aacaggtgcc
    14821 acccagaacg tcaaagacct gctttcctcc ccggaaattg aaaatatcct tgaaaacagc
    14881 gacttcatta cgctgctcaa tcaggcgtcc ggggatagaa aaatccttgc ggaacgcttg
    14941 aatttgtcca ccgaacagca gaagtacatc gacaattccg aacccggcga gggccttttg
    15001 atctttgaaa acgtggtgct gccctttacc aaccccatcc cgcacaacac ccagctttac
    15061 aagattatga cgacccgctt aaacgaggtg gcgggagtat gacccggcga cgccacaaag
    15121 gaggtgaacc accatgaaag agctgaaagc caaggcgaaa gtgacgcagg ccatgacccg
    15181 cgacgggctg acgctggaaa atcaggccac cggcgaggtg gagaacattt ccagccggga
    15241 ggccgaacag gatttgtccg cgccggggcc gggcggcacc gccgataagc tgctggaacg
    15301 ggccgacgcc atccatgacg gccataaggc caagaaagcc gcgaagcagg ccggggctgt
    15361 ggccgccgag ggggacgctg ccctgcaccg gccatccgcc cggctccaac tgtcggagga
    15421 agaacgggcc gacccggatt tacagcccta tatccgaaaa tcggacaaac gggccgataa
    15481 gctggataag gcccgcgccg ccatccccaa gaagcgcgtc ccggtcaagg aaaccgtctt
    15541 tgacgaggca agcggcaaag caaagaccgt cctgcacttt gacaaacagg acaaggggcc
    15601 tccctccctg aaacccaatc cggcaggccg tcctgtttcc gaggtttttc tattcggcca
    15661 cgggaaaatc catgaagtcg agcatgaaaa cgtgggtgtg gagggcggcc ataaggggga
    15721 ggaacttgcc gagcggcagg ccggaaaagc catccgcagc accatccggc accacaggct
    15781 gaaaccctac tgggacgcgg aaaaggccga gcgcaggcta ttgtctgcca acgcggagta
    15841 tttctaccag aaatctttgc gggataaccc ggagatcgca caggcggcca gcaaccccat
    15901 ttcccggttc tggcagaaac agcagatcaa gcgccagtac gccaaggccg cccggacagc
    15961 gggccagacc gcaggcgcgg cggcacaggg cgcggccaag acagccgggg cctccacgaa
    16021 agcggccaag gccgcagcca aagaaagtga acgggcggcc tcctttgtgg cccgccactg
    16081 gaaaggggcg ctggccgtcc ttgtgttggg gcttttgatg ctgcttatca tcggcggctt
    16141 acagtcctgc accgccatgt tcggcagcgc cggaagcggc atcacggcgt cctcttactt
    16201 ttccgaggat agcgatatgt tggccgccga ggacgcctac gcggagctgg aagccgagct
    16261 gcaagactat ctggacaact acgaggccac ccatgactat gacgaatacc attttgacct
    16321 tgacgagatc agccatgacc cctatgtgct gatttccatt ctgtcggcgc tccatgacgg
    16381 gatatttacc atcggggagg tacagggtga tttgtccatg ctctttgaca agcaatatat
    16441 cctgacagaa aacgtcgtgg tagaaacccg ttaccgcacc gaaaccgata catggacgga
    16501 cgcggagggc aacacccata cagacaccta ccaagtgccg tatgactatt acatctgcta
    16561 cgtcacgctg gaaaacttcg acctgtccca tgtgccagtc tatatcatgg acgaggaaac
    16621 gctaagtttc tattccctgt atatgtccac gctaggaaac cggcctgatc tgttttccgg
    16681 cagccagtac cccaacgcct ccaccctgac gccgccgacc tactacgacg tacccccgga
    16741 ggctttggag gatgccacct ttgccgccat gctcaaagag gcggaaaaat atatcggtta
    16801 tccctatgtg tggggcggca gctctcccag caccagcttt gattgcagcg gcttcatttc
    16861 gtgggtaatc aatcatagtg gatggaacgt gggacggctg ggggcgcagg gcctatataa
    16921 catctgcacc cctgtttcca gttcgcaggc caagcccggc gaccttgtat ttttcaaggg
    16981 aacctacgac acccccggcg tgtcccacgt cggcctgtat gtgggcaaca atgtgatgct
    17041 gcattgcggg aacccgattt cctacacgaa ccttacttca aactactggc aatcccattt
    17101 ttacagttac gggcgtttac cttaacgcca gaaaggagct ttcaattatg gcaaccaaac
    17161 ttgaccgcat cgaaaaggac atccagaaaa ccaagtctaa gatcgcggag tttcagaaac
    17221 agcttcggga gctggaaacg cagaaaaccg aacaggagaa tttgcagatc atccagcttg
    17281 tgcgcggcat gaacatgaag ccggaggaat ttgccgcgtt cttacgcagc ggcgcaatgc
    17341 aggccgcccc tgccgcaacc ccttaccatg aacaggagga caatgcccat gaagaataag
    17401 accagacgga tttcccgtat tctccccgca cttctggccg tgatgctctg cgtcacggcc
    17461 tttcccgtta ccgcccttgc aggcggcaat gacccggcac cggccccgct gccggaggct
    17521 acaaccgaac ccgctacggg cggtttggag cctgaaacag acgaaaccgg caccgccacg
    17581 gacgggacag aaaccgaggc cgaaaaagat accggccctc tgaccggcaa ggacatcacc
    17641 gacctgtttt ccgcgctgtt cggttccaag gtgagcattg ccgccacgga cgacggcatc
    17701 cagatcacca ccggcaccga caaaaaagaa cctgaacaga ccggcaccgt caccaccaac
    17761 ggcggcaggc tgaatgtccg caccggcgcg gggcttgaca agaccgcgtt cacccagctt
    17821 cccaatggga cgacggttga ggtagtcggc acagacggcg actggattaa aattctcttg
    17881 ccggagcgca tcggctatgt ccattccgac tatatgaccg tcagcgaaaa ggaagtagcg
    17941 gctaccggcg agggcggttt ttccctctcc attgacccgg aggaaattgc ttctctgctg
    18001 gaactgttca atggcggcgg ggccagcgcc gccctgtccc cggacgggaa cctgtcactg
    18061 attgacgaca tcggcagcgc cgcgaaaaat ggcaagcagt ttatcaccgt ggaaaccaaa
    18121 aacggcaatg tgttttatct gattattgac cgggacgacg agggaaaaga aactgtccat
    18181 ttcttaaatc aggtggatga agccgatctg ctgacgctga tggaggacgg ggaaaccgtc
    18241 acccccgctg ctgtctgctc ctgtaccacg aaatgcaagg ccggggctgt gaacacgaat
    18301 tgcccggtat gtaaaaccaa cctgaccgag tgcagcggcc cggagccgca ggaaccccag
    18361 ccggaggaac ccgaagcccc ggaggaagaa cccaagggcg gcgcgggcgg cctgatcgtg
    18421 ttcctgctgg tggcgctggc gggcggcggt gcggcccttt actatttcaa gttcaaaaag
    18481 cccaaggctg aaaccaccgg cagcgacgat ttgggcgagt atgattttgg ggaggacgag
    18541 gatttggagg acgaggaacc cgaaaccgag tttgacccgg aagctgattt tgtgagtgaa
    18601 caggaggacg aggaatgaca tataaacttg taatcgcgga aaaacccagc gtggcccgca
    18661 gcattgcggg cgtgatcggg gccacggaga aacacgacgg ctatttggag gggaacggct
    18721 atctggtgag ctggtgcatc ggacatctgg tgtcctttgc cgacgccgga cgctatgacg
    18781 agcgtttcaa aaaatggcgc tatgaggatt tgcccattct cccggaaaca tggcagtaca
    18841 tcgtccccga cgacaagaaa cagcagtttg acgttctctg ttcgctgatg cagcgccccg
    18901 acgtgaccgg ccttgtgtgc gccaccgacg ccgggcgcga gggggaattg attttccgtt
    18961 tcgtctacca gatggcggga tgccaaaagc cctttgagcg cctttggatt tcctctatgg
    19021 aggacagtgc tattaaggac ggctttgccc acctgaaacc gggcggcgac tatgatccgc
    19081 tgtatcaatc ggccctgtgc cgggccaagg ccgactggct gatcggcatt aacgccaccc
    19141 ggcttttttc cgtcctgtac cacaagaacc tgactgtggg ccgcgtccag acgccgacct
    19201 taaaaatgct ggtggacagg gacgccaaga ttaccgattt caagaaagag aaatatcaca
    19261 tcgtccatat cacggcgggc ggcgcggacg cggtaagcag ccgcttttcc gacgctgccg
    19321 aggcaaacac cgtcaaggcg gcttgtgcaa aagcgcaggc cgtttgcgtt tccgtgacgc
    19381 gggagaaaaa gacggaacag ccgcccaagc tctatgacct taccaccttg cagcgggagg
    19441 ctaaccggct tttcggctat accgccaagc agacccttga ctatgcccag gcactgtatc
    19501 taaagaagct gctgacctat ccgagaacgg atagtcaata tcttaccgac gatatgctgc
    19561 ccgccgtgga aagccttgtg tccggcctgt gggagctggt gccgtttgcc aagggcctga
    19621 acctttcccc gcagtttgac cgcatcctga acagcaaaaa ggtgtccgac caccacgcca
    19681 ttatccccac cgctgaattt gtgaagcagg gctttggcac tctggccgag agtgaaaaaa
    19741 agctgctgtc cctgatctgc tgcaagctct tatgtgccgt ggccgggccg catatctatg
    19801 aggccgtcac cgccacattt acctgtgccg ggcaggagtt taccgccaag ggcaagaccg
    19861 tcctttcccc cggctggaaa gagattgaac gccggttccg ttcttcctta aagaccgacg
    19921 ccgacgagga cgccgaggcg gtgcgggagc tgccggagct ttgcgagggc cagacctttg
    19981 cagacgtggc cgcctctgtc accgagcatt tcaccacgcc gcccaagccc tacaccgaag
    20041 atacgctttt gtccgctatg gaacgggccg gggccgagga tatgccggag gatgccgagc
    20101 gcaagggatt aggcacaccg gccacccgcg ccgccattct ggaaaagctg gtgcagatgg
    20161 gctttgtcca gagaaaaggc aagcagctca ttcccaccaa ggacggcatc aatctggctg
    20221 tggtgctgcc ggaggcgctt acttctcccc agcttactgc cgaatgggaa aaccggctga
    20281 ccgagatcgc caaggggcag gccgatcctg acgaatttat ggacggcatc gagacgcagg
    20341 cgcgggagct ggtgcagacc tattcctgta tcagtgagga aaagcagaaa ttgtttcagg
    20401 ccgagcgtgt tcctatcggg acgtgtcccc gctgtggtga ggctgtctat gagggcaaga
    20461 aaaactacta ctgcggaaac cgggcctgcc agttcgtcat gtggaagaat gaccgatttt
    20521 ttgaggaacg caagaaagcg ttcaccccga agatcgccgc tgcgctgctc aaaaccggca
    20581 aagtcaaggt aaagggcctc tattccgtaa agaccggcaa aacctacgac gggacggtgc
    20641 ttttggctga taccgggggc aagtacgtca actaccgcat tgaacacagg agctaaagga
    20701 cagtaacaca gcaagcatag ggagtgtggc tacactcccg gtaaaatccc ccggccgcgt
    20761 ccctgccggg ccggggattt ttgcttgttg gggaagtccc caacccccta ttttttgaaa
    20821 cagaacagag aaaggagtga ccgcgtgaac gaagtatttt ctatccaagt ccataaccgg
    20881 cagtttttgg aacagggcaa acaaggcgtc tggctggatt tgcccaccac aaccgaaacg
    20941 ctgcaagccg ccctgcggga tgttggcatt actgccgaca atccgcagga tttttttatc
    21001 aacgggtatt cctgcccgga ggacagacac cttgccattc cctatgacat ggtgctggcc
    21061 gccgacgtgg acgagctgaa tttccttgcc gcccggctgg gccagcttga cgccgccgag
    21121 atcgcagagc tgaacgccgc cctgcaaaat ccccgtggcg gctttgagaa tatcggccag
    21181 atcatcgact acaccgaaaa tgtggattac tatgtgcatc ttcccgacgt taggggggat
    21241 ggacagttgg gcgactacta cctgaaccgt tccggcatgg tggatatgcc ggaggaatgg
    21301 aaagccggga ttgatacggc gcagtttgga cgccacattg cccagcagga acaaggcgtt
    21361 tttacccagt acggctatct ggtgagaagc ggcgacgaat ggcagcgcgt ccatgagggc
    21421 cagccggtgc cggaggaata ccgggtgatg cactaccccc agccggaagc tgaccgggag
    21481 gaaccgcagg cccagcagcc ggaggcatcc gaaagccccg acaaagcgcc gcccccggtt
    21541 atcccgatca tgctggacgg catgaacagc aaagaacgca tgaaagtgat tactgaccgt
    21601 ctggaaaccg gcgtacagga gctttttgaa agtagccgct atatggctta cttatccgct
    21661 atgtccaaga tgcacgatta cagtctgaac aatacgctgc tgatcgccat gcagggcggt
    21721 tcgattgtgg cgggctataa caggtggcag gatgaatttg accgccatgt gaagcggggc
    21781 gagaaaggca ttaagatact ggccccggcc ccgttcaagg ttaagcagca gatggagaaa
    21841 atcgacccgg ccacccagca gcccgtgatc ggcgcggacg gcaagcccgt gaccgaagaa
    21901 caggaagtga cggttccggc ctttaaggtg gtgacagtct ttgacatcag ccagaccgag
    21961 ggcaaggatt ttcccatgcc ctatatcagt gaacttaccg gcgacgtgca gcagtatcag
    22021 gatttctttg ccgcgctgga acggacatca ccctatccca taagcattga gccgatttca
    22081 gacggggcca agggccgctg cttctacgca aaacagcgca ttgccatcaa cgagggcatg
    22141 agcgaattgc agaccctaaa gaccgccatc catgagattg cccatgccaa gctccacgcc
    22201 attgaccggg aggcccccgc cgaggaacag gcggcccggc cagaccgcag cacccgcgag
    22261 gtacaggccg aaagcgtggc ctataccgtc tgccagtatt acgggctgga cacgtcggac
    22321 tattcttttg gctatgtggc cgattggagc agcggcaggg agttatccga gctgaaagcc
    22381 tcccttgaaa ccatccgggc cacggccagc gaattgataa ccgagatcga cggccatttt
    22441 gcccagcttc agcaggagcg cgaggcccgg caggaacagg aggccgtcac ggttgacgtt
    22501 accccggagg cagcggcccc ggcgcaggcc gaggaagaac aggccccggc ccttgatccg
    22561 gcagccgagc cgcttgtgac tgtgctatgg agcgaaagcc cccagctaaa ggacggccag
    22621 caaatgccgt tgcatgaggc cgacgcgctg tttgcccgtc tggatgcagc atatccggca
    22681 gaggcaggct atgacaaaac cgcgttccgc attgactgta tctttcaggg cgagccggac
    22741 agctacgagg ggcggcagga ttttggagat agggacggtt ccctgatcga acatatccag
    22801 agctaccacc aattttacgc gcaggacgaa aactggaaaa agcatgtaat cgctacaacc
    22861 ggcatagagg gctgggagca ggacaaggcc catcgggata tgctgttagg cgagtttgtc
    22921 ccctatatga agctgcattg cagccttgcc caaatggaac agacggcgca gggccgctta
    22981 cagtccgggg aaaccctgac gccggaggaa acggcctatt tgggggcgct tctctcttat
    23041 gtgcaggagt gccgccccat gctcaatcag ggccagtatc agttgccgga gccgccgaaa
    23101 ctggctgact tcgaccagag cttgcaggac tacaaagcac aggtacaggc cgagatcgcg
    23161 caggaggccg ccgcagccgg tatgaccgtg gaggaatacg cggcggctgg ctatgaagca
    23221 ccccaacagg acaccttttc catctaccag cttcgaggcg gggaggaaac ccgcgactat
    23281 cggtttgagc catacgagcg tttacaggcc gcagggctgg ccgtggacag tgccaactat
    23341 gaccttgttt atacggcacc gctggataaa agcacgtctt tggaggatat ttaccgcaca
    23401 ttcaacatcg accacccggc agattttagg ggacacagcc tttccgtttc cgacgtggtg
    23461 gtgctgcacc agaatggaca ggacaccgcc cactatgtag acagcttcgg ctatatggaa
    23521 gtgccggagt ttttgcggga aaatcccctg cgtaccgccg aactgtctac ggagcagaac
    23581 gaaaacatga ttgacggcat tatcaacaat acgccgcccg aaccgcagcc ggaggaaccc
    23641 gaacagctat cttttgagga tgtggcagtt gcttccggcc agacacagga ggaacccccg
    23701 gaggctgcgc ccccgcaaac ggctgtccgc tattatccca tcaatgaggg agccgcccgc
    23761 cgcgccaagg aagccatgag cttttctgac taccggcccg gcagcgccac ggcggaatac
    23821 cggcactatg tcgatcaggc cgtggaaatt gcggagcgcc agaaaaagcg tgtggagcca
    23881 gagtatcacg aaaagataga cagcctcctt gacacctacg cccgcaagct ggccgagaac
    23941 atgaataagg gctatgagat taccgcccgc gtcccctctg tcctgattgc cgggccgtcc
    24001 aatttcccgg tacgggcgaa agaaaagcag aacgccgcca gtgaccgcaa catggaggaa
    24061 tttcagtaca ttcagggctt gcttgacaaa atccgcagca ccggcatggg tggtatcagc
    24121 gccgacgatc cgcaggccgt ttccaagctg gaaaagaaac tggaaaagct ggaagcgtcg
    24181 caggagttga tgaaggccgt caatgcctac taccgcaagc atgggacgct ggacggatgc
    24241 ccccatctga ccgaaagagg cattgaaaac ctgaaagcgg atatggcgtc gggctggcac
    24301 tatgagaaaa aaccgttcca gtcctggcag ctctccaaca ataacgccga gatacgccgg
    24361 ttaaagggcc gcattgagga attgacccgc caaaaggagg cggcctatgt gggatgggag
    24421 tttgacggcg gcactgtgga gatcaaccgg gtggccaacc gcctgcaaat cttctttgag
    24481 gggaagccgg atgctgctgt acgcgacgag ctgaaaagca acggtttccg ctggtcgccc
    24541 aaggccgggg catggcagcg gcagttaaac gacaccacaa tccgcgtcgc tgaccgtatc
    24601 aagtgcatcc agcccctttc cggcgaaaag cccagcgcct tacagcttgc ggcccgcagg
    24661 gagaaagaaa agccctctat ccgggcgcag ctcaacgcgg ccaagaccga acaggccaag
    24721 cagccaccgg cgcgggaaaa atcaaaggga ttggaggtag agtaaatgca gaagtttgaa
    24781 aatgtggacg ttctggccgc gctggaacag atcatgcggc agaacaccgc gttttatcaa
    24841 agtgactttg gcattgacaa agacattatc aggcgggatg ccgccagcga tcaggccgca
    24901 gacaagaccc tgctctggat gtcccggccc tccgggacgt actgtttccg ggaacgtgat
    24961 gttttcctga aagacacccg acagtacaac acatggaaat tttacgggga gcagacccgt
    25021 gacaagatac tggcctacgc ggtggagctg accggcacac agggcggcac tatccggggc
    25081 aatctctatg agctggacta tccgcagcat ttccggcacg tcattgaaac cgcccagccg
    25141 gtgagtgtga accggctctt ttatgagcat ggcacccgtg acatcccgga gagccagcgt
    25201 tttgacggaa gccctgaccg ggtattggga aactttttgt actatgaggc ccagccccat
    25261 gacccggctg tgctgcagga ggcgctacgg acggagcagc acagccgggg gcgggccgtc
    25321 cccggcgatt tcaaagtcca cgtcaccgct ttgcatgaca gccggattga aaccgaggcc
    25381 cggcgtattg tggacagcct gaaagatctg tccgcgccta acagccccaa taaaacgcat
    25441 ttcatggtgg agatctcccc gcactttgcg gcgattgctt ccaccaagga taatgaccgg
    25501 ctctttgcca tgctgcccta taaatccctg tgttttaccg ggatgaaagg caggcatggc
    25561 gtgtatgccg tgattaaccg ggacgagcgc cgggatgtga gtattcgcag gccccggccc
    25621 tctatccgca agcagctatc cgacagcaaa caggccgcca gcccaaaaaa ggcggcggcc
    25681 agaaccaaaa agaatgaatt ggaggtataa ggaatgatgc actttaccgt ggaggaagaa
    25741 aacctgatct gtatgtacca caatgcagac cggcgcagaa cgataaacag gctcaccgcc
    25801 gtcatgccgg ttatggatgg ggatatgcgg gcgcaggccc agcagaccct ttccaagctg
    25861 gaacgcatga cggatgccga ctatgacggc cagaagttcc acttcacaga cgaataacga
    25921 acagcagacg ccgggcgcgg cggccatgtg gtcgcttgcg tccggcgtct tttctttttg
    25981 cctattccta cgcatttaga acccggtatt gtcggggata ggatgaagta ttcgcttgcc
    26041 cctgcaaaca tgcgggagaa gccttgcagc gggcggtttt caatccctta aatgcgtagg
    26101 ttgcagctat aattagaccc ctgaaacaga tatagccgcg cagcgggtgt atctgtttca
    26161 ggggtgtgca ggtgatagcc gcaaagcggc gtaagagggt gcagccctct tgccgtctat
    26221 ctctgttcca gcgggaagct cggcaatgct tccagcagtt tcagggaaat gcgctgccgt
    26281 atatcttcgt caatgacttt cttctttttg ccgtttgcct gtctgatctc caccgtggaa
    26341 agctcgttga tgtagtcagc ataatgggcc aacacttttt ccgtggccca cctctcaccg
    26401 gcaacggcgg ctttgatcgt ttcatagtcc agcagctttt cttgtccgtc catcctgata
    26461 cccccgtcag actgattttt tctatgatac catattcgga agcatggaac cagagaaaac
    26521 ggcgcttttc tgctggtaaa ttctacgcat ttagaacagc acttcggcgg ggataggata
    26581 aaacacccac ataccccgct gaccatgccg gaaaagcctt gaaatataag ggcttttaca
    26641 gctctaagtg cgtaggtttc cagtatcttt tccgctggcg ctcggcctct ttgcggcggc
    26701 gcatccgttt ggagcaggcc gcgcagtatt tggcccggtt agacttcggc gtgaacgtgg
    26761 ccccgcactc acagcagcgt ttcacttcgt cccggtcttt ggtgatctcg gcatacaggg
    26821 ccgcatccag cgggaggaca gcggccttaa accagcggca cagaagcgaa taggaaatgc
    26881 tctgtacgca aatacattct tcgccgtcgt ccagcaacag gcagttgccg tcacaatagt
    26941 tgcagcactc atgcgtcagc ttccgggcct tgcgatattg agggtaattc attcgtggaa
    27001 tactcatagc tcttgcgtcc attcctttcc cggcgtcata cgcaaaatgc cgtccacatt
    27061 ctgcttgatg atgccgtatt cctgcatctg ctttttcgct tcgccgtact tctcatacag
    27121 ccgttccttt tcctcggcca atcgcttgta ctcggctttc agggcgtaca gatcggggag
    27181 ctttttcaca ccctgtccct ttagggcctt tgccgcagct tcatacaaaa tcaacgtgct
    27241 ttcatgctca cgccggaatt ttgccttatc cgctgcattg cggtattcca tagccacggg
    27301 ccagagctgc ttgtaggtag aaatattctt tatcagcacc gccatatccg caagccggtg
    27361 ttccatagct ttgagggtag cggcggcctc gtcgctggcg gcggtgatct cggccacttt
    27421 gctttccaaa tccgggtagg tgtctatccc atgttcagtc aggaaattca aagtctttgc
    27481 ggcctgcttc aaattgtgga gcttcgccca cctctcatag cctgccgact gctgggcctt
    27541 aatgttgttt tccagatcaa tccgcaaaga aatccccttg cgctctttgg gcgctctggt
    27601 gataacgcgg cctttgatac gctctttgat ggcttcctcg gtgtaggctt cccccaatgt
    27661 cttgcagcgg gtaaagcgtt cctgtccggg agcgcggaaa gaaataaact ttcccggctt
    27721 gatctcatag ccctgcgcct gcatgagccg caggaaatca tcaaaatctt ttgcctgcgg
    27781 gatagcggcg tcaatggctg ctttgagctt cgctttccag ctcgtccctt tccgctgggc
    27841 gtcccactcg gcatagctct tgcccttact ctggccgggc ttcaccacgg acaatccgtt
    27901 ttccttgcac agcctgtcgc tggtgcgccg gatataggca tagctctgtt tgttggaaat
    27961 gtacttgcgc tgatttacca tatccacggc acagaaaata atgtgattgt ggacgtggcc
    28021 cttatcaata tgggtagtca gcacataggg atatttccct tgcagcactt cctcggcaag
    28081 ctgcttcccg atctcatggg cctgttccgg cgtggcttcg ccgggggcaa aggattgtat
    28141 caggtggtgg cccagattat tgcccttttg gtatgcctgc gccagcagca tttcaaattc
    28201 aatgtccgct gtttcataag agcaggcaaa ggaggccacc agcagcttgc cgtcagtttt
    28261 gtccgggttc tcgatgtagt caagggcttt tttcagcgtt gacttgatag gatgtatctt
    28321 tgtaactgcc atatctgcgc cagcgccccc tttatatcct cgatgtcctg tgcgtacacc
    28381 ggccccatac cgttcagcct ctttgcaatc tgattgacgt tgacgccgat cttctgtatc
    28441 tcggcggtct gcgcccggat gtcgctggtg tccatgttga cgatatagcc gtcaatgagc
    28501 atcttccggg cgtaggctga aaagttcttc gtatgcagca gcgccatttt ctgttcaatc
    28561 atagcccgtt cctccggcgt cacagggacg cgcagcacga tttttcgctt tctgtttgcc
    28621 atgcttcacc gtcctttcca ttcaggggtt tggggatttc cccaacaagc aaaacggacg
    28681 cggcccggca gggacggggc aggccgtttt tccgggagtg gctacactcc ctatgcttgc
    28741 tacgttactg tttctccact aacaagacgt ttccaaagcc ccaaactacc cgcaaaatag
    28801 ccctctggaa agaaaaaagt gcaaaaaaat agaagctacg ctgtgcagct cccgctaaaa
    28861 gtaaaacaga ggatgccagt tacacatcct ccgtttcatt cttcttcggc ttttcaatta
    28921 gtatggcgct gtcaatcgcg ccctcgatga tcggcaggta ttcttcgggg cagagtttca
    28981 gcttgtggct gaccctttgc cgcagttcgc tttcctcccg catgatctcc gggttaaagt
    29041 agcgttccac aggaagcccg caggctttaa taagctgtat gacaaccggc agactgggaa
    29101 tggccccgtc gttttcgatg ttggcaagat agcgccagtc aatatttatt ttctctgcca
    29161 ttgctttgcg cgaaacgcgc tttgcttgcc gggccgcttt cacatccgcg ccgaaagttt
    29221 caaagccggg acaatcttca atattcgcca tatcgcacct cccacttaca ttgtatattt
    29281 catactctgc ccgtggaatg ttggtatatg cacctttctt aaagtttata tttcacattt
    29341 tgtttctttt attcttcgag caaaagaatt ataatgtacc ctgtggaggt gtactatgga
    29401 ctacatgacg ttgaaagagg caggcgagaa atggggtgtg cccccacgat ggataaacta
    29461 ctattgtgct gcgggacgca tccccggcgc tgtaaagatg gcgacgatct ggctcattcc
    29521 caaaactgcg gaaaagccga ttgacaggcg tacaaagaaa ggaaaggcgg ttactgaata
    29581 gtataatgaa accggcgcat tttggatatg gttttgaaga gagagaaagc atggtatttt
    29641 gtcacggaaa aactataaga tgatagatgg caggctgctc cagaccgata aaaagtattc
    29701 tcatctgaaa cagaaagaaa agatttacac atggatgtat gaagaagcga aactttatca
    29761 tgacagaaat ggaaaatgcc cgaaacaaag acgaggaagc tgctgctgta tcagccgtat
    29821 atggtagaat tgaaaaggcc ggcatctgga ttctttatgg tgaagtctcc agacattgga
    29881 taactgctgg tctaatgcgc ctttgagtat ggggtgattg attttaatac cggggaggta
    29941 cggctttttg aggaaaatat tctgggattt acatttggcg attattacat ttcctgtaac
    30001 tgcaaggaca cggatgatga agatatgagg aatatagact tgctgatttt ctactgtctt
    30061 gaacggtatt aggactgaat ctgctgcgcg gagtgcggca agagcgaccg gagggagtac
    30121 acacaggccc tgcctgtgtg aaaagccata ccgcaaggtg cattcgcaaa aaagaggccg
    30181 cgcattttgc tgaggcttgg ggtgtcccca acaagcaaaa cgagaacgcc gcaggcgggc
    30241 aagtggtgga tcagaatatc cgtaaaggat attctgatcc attgcttgcc agtattatcg
    30301 ggatgttttg acaaccgctg taatcgaaac atcccaaaca ttaagaaggt gttaattatt
    30361 attatcttga tttaattcgt ttaaaccaat aatatagata catacaccat gaaaagaagc
    30421 gagtatccca atgacggatg attaaaatcc gaaaagaaat cgctttgaaa ctacagggaa
    30481 actacagact gttatgttat cttcttaaaa tggagggatt tttatgtcga tacgaattct
    30541 acttgtcgag gatgatgatc atatctgtaa tacagtaagg gcgtttctgg ctgaggcagg
    30601 atatcaggtg gatgcctgca cagatggaaa tgaggcatac accaagtttt acgaaaacac
    30661 ttatcaactg gttattcttg atattatgct gcccggtatg aacgggcatg aacttttgcg
    30721 tgaatttcgt gcgaaaaatg atactcccat tctgatgatg acagccctgt cggatgacga
    30781 aaaccaaatc cgggcgtttg atgcagaggc agacgactat gtaacaaagc cattcaagat
    30841 gcagatttta ctaaagcggg tggaagccct gctgcggcgc agcggtgcgc tggcaaagga
    30901 aatccgtgtc ggcaggctga cacttctgcc ggaggatttc acggtacttt gtgacggtac
    30961 agagctgccc ctgacacgaa aagaatttga aatccttttg ctgctggtgc agaacaaagg
    31021 cagaacctta acgcatgaaa tcattttgtc acgtatatgg ggatatgact ttgagggtga
    31081 tggcagcaca gtccacactc atatcaaaaa tttgcgggcc aagctgccgg aaaatatcat
    31141 caaaaccatt cgcggtgtag gataccgatt ggaggaatca taataatgga aagaaaaggg
    31201 attttcatta aggttttttc ctatacgatc attgtcctgc tactggttgt cggtgtaaca
    31261 gcaacactgt ttgcacagca atttgtgtct tatttcagag tgatggaatt acagcaaaca
    31321 gtaaaatcct atcagccatt ggtggaactg attcagaata gcgataggct tgatattcaa
    31381 gaggtggcag ggctgtttca ctacaataac caatcctttg agttttatat tgaagataaa
    31441 gagggaagcg tactctatgc cacaccaaat gccaatacat caaatagttt tagacccgac
    31501 tttctttatg tggtacatag agatgataat atttcgattg ttgctcaaaa caaggcaggt
    31561 gtgggattgc tttatcaagg gctgacaatt cggggaattg ttatgattgc gataatggtt
    31621 gtattcagcc ttttatgcgc gtatatcttt gcgcggcaaa tgacaacgcc gatcaaagcc
    31681 ttagcggaca gtgcgaataa aatggcaaac ctgaaagatg taccgccgcc gctggagcga
    31741 aaggatgagc ttggcgcact ggctcatgat atgcattcca tgtatgtcag gctgaaagaa
    31801 accatcgcaa ggctggagga tgaaatcgca agggaacatg agttggagga aacacagcga
    31861 tatttctttg cggcagcttc tcatgagcta aaaacgccca tcgcggctac aagcgttctg
    31921 ttggagggaa tgcttggaaa tatcggtgac tacaaagatc attctaagta tctgcgcgaa
    31981 tgcatcaaaa tgatggatag gcagggcaaa atcatttccg aaatactgga gcttgtcagc
    32041 ctgaatgatg ggagaatcgt acccatagct gaaccgttgg acatagggcg cacggttgcc
    32101 gagttgctgc ccgattttca aaccttggca gaggcaaaca accagcggtt cgtcacagat
    32161 attccagccg ggcaaattgt cctgtccgat ccgaggctgc tccaaaaggc actatccaat
    32221 gtcatattga atgcagttca gaacacgccg cagggaggcg aggtacggat atggagtgag
    32281 cctggtgctg aaaaatgccg cctttttgtt ttgaacatgg gcgttcacat tgatgatact
    32341 gcgcttccaa ggctgttcac cccattctat cgcattgatc aggcgcgaag cagaaaaagt
    32401 gggcgaagcg gtttaggact tgccatcgta caaaaaacgc tggatgccat gagcctccag
    32461 tatgcgctgg aaaacacctc ggatggcgtt ttgttctggc tggatttacc gctcacatca
    32521 acattgtaaa tatttaaaac ttaaatgatt ttgaccgaca ggtataaccc tgccggtctt
    32581 tttttgctgc tacaggaaaa ctacagactg gctacaggga aagtacagat gcgcttgcca
    32641 taataacaat cgtaccagcc acaaatcgta gttttattgc aaaggaggca ttcgatcaaa
    32701 tggaaaaaag caactatcat tccaatgcgg atcatcacaa acggcatatg aaacaatccg
    32761 tggaaaagcg ggcttttcta tgtgcgttca ttatttcgtt cacagtctgc acgctgtttt
    32821 tggggtggag actggcttcc gtattggagg caacacagat accgcccatc cctgcaactc
    32881 atacaggcag cagcactgac gtagtggaga atttggagga aaacgctctt gccaccgcca
    32941 aagaacaggg agatgaacag gaatggagcc tgattttagt gaacaggcag aaccccatcc
    33001 ccgcacagta cgatgtggag cttgagcaac tatcaaatgg tgagcggata gatattcgga
    33061 tttctcccta tcttcaagat ttgtttgatg ccgcaagaac tgatggagtt tacccgattg
    33121 tcgcatccgg ataccgaaca acagaaaaac agcaagaaat tatggatgaa aaaattgccg
    33181 aatataaggc gaaaggctac acctctgcac aggctaaagc ggaagcagaa acttgggtgg
    33241 ccgtgccggg aacgagcgag catcagcttg gtcttgctgt ggatatcaat gcggacggaa
    33301 ttcattcaac aggcaacgag gtttatagat ggctggatga aaacagctat cgttttggtt
    33361 ttattcgtcg ctacccacca gacaagacag agataaccgg tgtgagcaac gagccgtggc
    33421 attaccgata tgttggcatc gaagctgcca cagagatgta caaccaaggg gtttgccttg
    33481 aggaatattt aaaaccagaa aaatgagaaa aggatgttat gttatggaca gaaaaagatt
    33541 gacacagcgt ttcccgttcc tgcttccaat gagacgggcg cagagaaaaa tgtgctttta
    33601 tgcgggaatg agatttgacg gctgtcgcta tgcgcagacg ataggggaaa aatcgctttc
    33661 ccatttgctc tttgaaacgg attgcgcatt atacaaccac aataccggat ttgacatgat
    33721 ataccaggaa aacaaggtgt tcaacttaaa gctggcagca aagaccttaa acggcttatt
    33781 gataagaccg ggggaaacct tttctttctg gtggctggta cgccatgcgg acaaagatac
    33841 cccctataaa gacggcctta cggtgaccaa tggtaaactc accaccatgt cgggcggcgg
    33901 tatgtgccag atgagcaatt tactattttg gatgttcctg catacgccat tgacaattat
    33961 ccagcgcaga ggtcacgaag taaaggagtt tccagagcca aacagtgacg agattaaagg
    34021 ggtggatgca accattccag agggctggat tgatttaaaa gtgcgaaacg ataccgactg
    34081 cacctaccaa atatgggtga ccttagatga tgagaaaatc atcggtcagg tgtccgccga
    34141 caaagagccc caagcattat acaaaattac aaatggcagt atccagtatg tccgtgaaag
    34201 tggcgggatt tatgaatatg cccaggttaa acggatgcaa gttgccttag gtaccgggga
    34261 aataatagat tgcaagctgc tttatacaaa caaatgcaaa atctgttatc ccctcccgga
    34321 aagtgtggat attcaggagg agaaccaatg agaaaaagtg tgggcattac tgtttttgga
    34381 tgcgaacagg atgaggcaaa tgctttccgc gctttatcgc cggattttca tattatccct
    34441 acgctgatta gcgatgcgat atcggcagac aacgcaaaat tggccgctgg caatcaatgc
    34501 gttagcgtag gccataagtc cgaggtttcc gaggcgacaa ttcttgcgct gagaaaggtc
    34561 ggggtaaaat acatttctac ccgcagcatc ggctgcgatc acattgatac gactgccgcc
    34621 gagagaatgg gaatctcggt tggcacggtt gcgtattcgc cggacagcgt tgcggattat
    34681 gctttgatgc tgatgctgat ggccatacgg ggtgcaaaac ccaccatgca cgccgtggcg
    34741 caacaagatt tcagattgga tcgtatccgg gggaaagaac tgggggatat gactgtggga
    34801 gttattggaa ccggccatat cgggcaagcg gtcgtcaaaa ggctgcgggg atttggatgc
    34861 catgtgctgg cctatgataa cagccgaaaa atggatgcag attatgtcca gcttgatgag
    34921 cttctaaaaa acagcgatat tgttacgctc catgtgccgc tttgtgcgga tacccgccat
    34981 ctgatcggtc agaagcaaat tggagagatg aagcaaggcg catttttaat caacaccgcg
    35041 cgcggagcgc ttgtcgatac cggggcgctg gtggaggcgt tggaaagcgg aaagctgggt
    35101 ggtgcggcac tggatgtgtt ggagggcgag gatcagtttg tttataccga ttgctcgcag
    35161 aaagtgcttg accatccctt tttgtcgcag cttttaagga tgccgaatgt gatcatcaca
    35221 ccccatacgg cgtactacac tgagcgtgtg ctgcaggata ctacagaaaa aacaatcagg
    35281 aattgtctca attttgaaag gagtttacag catgaataga ataaaagtcg caatcatctt
    35341 cggcggttgc tcggaggaac atgatgtgtc ggtaaaatcc gcaatagaaa ttgctgcgaa
    35401 cattgatacg gaaaaattcg atccgcacta catcggaatt acaaaaaacg gtgtatggaa
    35461 gctatgcaag aagccatgta cggaatggga agccgacagt ctccccgcca tactctcccc
    35521 ggataggaaa acgcatgggc tgcttgtcat gaaagaaagc gaatacgaaa cacggcgtat
    35581 tgatgtggct ttcccggttt tgcatggcaa attcggggag gatggtgcga tacaggggct
    35641 gtttgtattg tctggtatcc cctatgtggg ctgtgatatt caaagctccg cagcttgcat
    35701 ggacaaatca ctggcctaca ttcttacaaa aaatgcgggc atcgccgttc ccgaatttca
    35761 aattattgat aaaggtgaca agccggaggc gggtgcgctt acctaccctg tctttgtgaa
    35821 gccggcacgg tcaggttcgt cctttggcgt aaccaaagta aacggtacgg aagaacttaa
    35881 cgctgcgata gaagcggcag gacaatatga tggaaaaatc ttaattgagc aagcgatttc
    35941 gggctgtgag gtcgggtgtg cggtcatggg gaacgaggat gatttgattg tcggcgaagt
    36001 ggatcaaatc cggctgagcc acggtatctt ccgcatccat caggaaaacg agccggaaaa
    36061 aggctcagaa aatgcgatga ttacagttcc cgcagacatt ccggtcgagg aacgaaatcg
    36121 ggtgcaggaa acggcaaaga aagtatatcg ggtgcttgga tgcagagggc ttgcccgtgt
    36181 tgatcttttt ttgcaggagg atggcggcat cgttctaaat gaggtcaata ccatgcccgg
    36241 ttttacatcg tacagccgct acccacgtat ggtggccgcc gcaggaatca cgcttcctgc
    36301 actgattgac agcctgatta cattggcgtt aaagaggtga taagtatgga aaatggtttt
    36361 ttgtttttag atgaaatgtt gcatggcgtc cgttgggatg ccaagtacgc cacatgggat
    36421 aacttcacag gaaaaccggt ggatgggtat gaggtgaatc gcatcatcgg cacaaaggcc
    36481 gtggcgtttg ctctgcgcga ggcacaaatc catgcggctg cgcttggcta tggcttgctt
    36541 ttatgggatg gatatcggcc aagaactgcg gtggactgct tcctgcgttg ggcagcgcaa
    36601 ccggaggaca atctcacaaa agaaaaattt taccccaata tagagcgagc cgagttgatt
    36661 acaaagggtt atgtggcctc acaatccagc catagccgtg gaagcgcaat tgatcttacg
    36721 ctctaccacc tggatacagg ggaacttgtt tcaatgggaa gtaacttcga ttttatggac
    36781 gaacggtcgc atcatacagc aaaagggata ggggatgcag aggcacaaaa tcgaagatgc
    36841 ttgcgtaaaa tcatggaaag cagcggattt cagtcttatc gctttgaatg gtggcactat
    36901 aagttgattg atgagccata ccccgatacc tattttaatt ttgccgtttc ataatgaaag
    36961 tattggtttt gtatcagatt ttggatttga taaaatctat gctggtcatg gacaatcttt
    37021 tactggaaag tggttaaagc atcttttgta atacattgca aattatagta ttcaatctgc
    37081 ccagcggcag aaaagaaaaa aaccgctgtt gggcagacct ttcccacgcc cattttgtat
    37141 tttgctggcg gcggttttga gaaatcaagg ccgccgttct tttctggcag caaaaggtgt
    37201 ggcggcccat aggaggatgc cgctatgctg ttttgctatt gccatgctct aatccacttc
    37261 caccactcaa aaaagcggct ctttgtcaat aaggactgat gagttgtgca aaatc
//
LOCUS       DAMYXM010000232        13213 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.guided.068, whole genome shotgun sequence.
ACCESSION   DAMYXM010000232 DAMYXM010000000
VERSION     DAMYXM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.guided.068"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(204..2162)
                     /locus_tag="PNS21_002996"
     CDS             complement(204..2162)
                     /locus_tag="PNS21_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDI5160609.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="PNS21_002997"
     CDS             complement(2159..3031)
                     /locus_tag="PNS21_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDI5160610.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="PNS21_002998"
     CDS             complement(3373..4245)
                     /locus_tag="PNS21_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI5160611.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="PNS21_002999"
     CDS             4373..4942
                     /locus_tag="PNS21_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDI5160612.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="PNS21_003000"
     CDS             5009..5746
                     /locus_tag="PNS21_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI5160613.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="PNS21_003001"
     CDS             5977..7245
                     /locus_tag="PNS21_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDI5160614.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="PNS21_003002"
     CDS             7732..8196
                     /locus_tag="PNS21_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDI5160615.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="PNS21_003003"
     CDS             8180..8809
                     /locus_tag="PNS21_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDI5160616.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="PNS21_003004"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="PNS21_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDI5160617.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="PNS21_003005"
     CDS             10688..11134
                     /locus_tag="PNS21_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDI5160618.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="PNS21_003006"
     CDS             complement(11333..11848)
                     /locus_tag="PNS21_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDI5160619.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="PNS21_003007"
     CDS             11965..13167
                     /locus_tag="PNS21_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI5160620.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DAMYXM010000233        12877 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.guided.069, whole genome shotgun sequence.
ACCESSION   DAMYXM010000233 DAMYXM010000000
VERSION     DAMYXM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.guided.069"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            14..238
                     /locus_tag="PNS21_003008"
     CDS             14..238
                     /locus_tag="PNS21_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI5160621.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            355..852
                     /locus_tag="PNS21_003009"
     CDS             355..852
                     /locus_tag="PNS21_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDI5160622.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            827..1333
                     /locus_tag="PNS21_003010"
     CDS             827..1333
                     /locus_tag="PNS21_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDI5160623.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            1317..2822
                     /locus_tag="PNS21_003011"
                     /pseudo
     CDS             1317..2822
                     /locus_tag="PNS21_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3567..5393
                     /gene="ltrA"
                     /locus_tag="PNS21_003012"
     CDS             3567..5393
                     /gene="ltrA"
                     /locus_tag="PNS21_003012"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDI5160624.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            5428..6444
                     /locus_tag="PNS21_003013"
                     /pseudo
     CDS             5428..6444
                     /locus_tag="PNS21_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6447..8624
                     /locus_tag="PNS21_003014"
     CDS             6447..8624
                     /locus_tag="PNS21_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDI5160625.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            8621..9622
                     /locus_tag="PNS21_003015"
     CDS             8621..9622
                     /locus_tag="PNS21_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDI5160626.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            9619..10551
                     /locus_tag="PNS21_003016"
     CDS             9619..10551
                     /locus_tag="PNS21_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDI5160627.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            10796..10912
                     /locus_tag="PNS21_003017"
     CDS             10796..10912
                     /locus_tag="PNS21_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDI5160628.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            10928..12847
                     /gene="tet(M)"
                     /locus_tag="PNS21_003018"
     CDS             10928..12847
                     /gene="tet(M)"
                     /locus_tag="PNS21_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HDI5160629.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT         4202 a         2338 c         2735 g         3602 t
ORIGIN      
        1 tgagatattt ataatatcag gaacttacct atccatatat aactggtttc tatcaaacgc
       61 tcaatcactg gtgcttttag caatcgttgt gattggcttg tatcttggct tcaagcgtga
      121 gtttagcaaa ctgattggct ttttaattat tgcgattatt gcggttggct tagtcttcaa
      181 cgctgctgga gtaaaagaca ttttactaga gctattcaat cgcattattg gtgcttaaat
      241 aaaaccgttc ttttgtggaa tataagtggt tttcttatgt tccgcaaagg aatggtacac
      301 caaacgaagt gcggtaggga tttttgaatc tctacaaaga aaggacgtga atatatggac
      361 gatatgcaag tctatattgc gaatttaggc aaatacaatg agggcgaatt ggtcggtgcg
      421 tggtttacct ttcccattga ctttgaggaa gtcaaagaga aaatcggctt gaatgatgaa
      481 tatgaggaat acgccattca tgactacgag ttacccttta cggttgacga atacacttcc
      541 attggcgaac tcaatcgact atgggaaatg gtatcggaat tacccgaaga attacaatcg
      601 gagctatctg ctctgctcac tcatttttca agcattgaag aactaagcga acatcaagag
      661 gatattatca ttcattccga ttgtgatgat atgtatgacg tggcacgcta ctacattgaa
      721 gaaacgggtg ctttaggcga agtaccagct agtcttcaaa actatattga ttatcaagcc
      781 tatggtcggg atttagacct ttcaggaacg tttatctcaa ccaatcatgg gatttttgaa
      841 atcgtctatt aaatctgtcg gtacattact actggcagat tttctatttt acggggtggc
      901 tcaatcagct acccctattt tttatgaaag gattgattac atgaagaaaa tacgaagcta
      961 taccagtatc tggtctgtgg aaaaggtact gtattctatc aatgatttta gacttccgtt
     1021 tcccataacc tttacgcaaa tgacatggtt tgtcgtgtca ctctttgcag tgatgatact
     1081 tggcaacttg ccccctcttt ccatgataga gggagcattt ctcaaatact ttgggattcc
     1141 tgtggctttc acatggttta tgtctacaaa aacttttgat ggtaaaaagc cttatggatt
     1201 tttgaagtct gtcattgctt atgcactgcg accaaagctg acctatgcag gaaaaaaagt
     1261 aacgcttggc agaaaccagc cacaagaagc cattacagca gttaggagtg aattttatgg
     1321 catatccaat taaatacatt gaaaacaatc tcgtctggaa taaagacggg gaatgttatg
     1381 cttactatga gcttgttcct tacaattact catttctaag tccagaacag aaaatacaag
     1441 tgcatgattc tttcagacag cttatcgcac aaaatcgtga tggcaaaatt catgctttac
     1501 aaatcagtac agaatccagc atacgttctg cacaagagcg ttccaaaaat gaagtcactg
     1561 gcaagctcaa agcggttgcc tatgacaaaa tcgaccaaca gacagacgct ttaatatcca
     1621 tgattggcga aaatcaagtg aactaccgtt tctttatcgg ctttaagttg cttctcaacg
     1681 atcaggagtt ttctatgaaa agtcttaccg ttgaagcaaa aaatgctttg tctgattttg
     1741 tctatgatgt gaaccataag ctgatgggcg attttgttag tatgagtaat gatgaaatcc
     1801 tgcgttttca gaagatggaa aagctcttag aaaataaaat ctctcgtcgt ttcaaaatcc
     1861 gcaggttaga taaggacgac ttcggctatc tgattgaaca cctttacgga cagacaggca
     1921 ctgcctatga agagtatgag taccatctat caaagaaaaa gctggataat gaaacgctga
     1981 ttaaatacta tgacttgatt aagcctactc gctgtttggt ggaagaaaaa cagcgatatt
     2041 tgaaaatcca gcaggaagat gaaaccgtct atgtagctta ctttaccatt aacagcattg
     2101 tcggagaact ggacttcccg tcctctgaaa tcttctacta ccagcaacag caatttacat
     2161 tcccgattga tacgtcaatg aatgtggaaa ttgtagcgaa tcgtaaagcc ctatctactg
     2221 tccgcaataa aaagaaagaa ctgaaagact tggataacca cgcttggcaa agtgataatg
     2281 aaaccagctc caatgtggcg gaagctctgg aaagtgtgaa tgagctggaa accaatttag
     2341 accaaagcaa ggaatctatg tacaagctgt cttatgtggt aagggtatca gcaaatgatc
     2401 ttgacgaact caaacgtcgt tgtaatgaag tgaaagattt ttatgacgat ttaagcgtaa
     2461 aactggtacg accatttggg gatatgctcg gcttacatga agaattttta cctgccagca
     2521 agcgttatat gaatgattat attcaatacg tgacctctga tttcctcgct ggtttaggtt
     2581 ttggtgctac tcaaatgctg ggggaaaatg aggggattta tgttggctac agcttagata
     2641 ctggacgcaa tgtctatctg aaacctgctc ttgccagtca aggggttaag ggttcagtaa
     2701 ccaatgcgtt agcgtcggct tttgttggtt cgctgggtgg tggtaaatcc tttgcgaata
     2761 accttatcgt ctattatgcg gtgctttatg gggcacaagc agtgattgta gacccaaaag
     2821 caggtgcgaa acgttcctac ttgaaaagag taggcacagc acttcattaa atcaatattt
     2881 aatgtgtgtt ggagaactaa acacacgaat agtcgaatga cggggtaacg ccctgaaagg
     2941 ctactttaat actctgactg gcagaaactt gagtaaacaa ggctctgtca gaagctcggt
     3001 gaagtcggca gacagacacc gtaacagata atgctgacga aagattccca gaggtgggcg
     3061 ttgtgtccta tatgccggaa gtctataaaa tatctatggt tagaatgtag atgagaggtc
     3121 tactgacgaa tccttgaatg tacaggtcta tatcggggct ggatagaaat gtccagtgac
     3181 acaaacagtg tcgctactgt gggtgagtaa gactccgcat ttatgaaaat ccatataacg
     3241 ttaaaggcgt tatcaaggta gcaggcttat agaggaaacc taaggatata tgtacagata
     3301 gtgtgtttgg aacgttgaaa gctgacaaca tggagatttt actgtctgtg aagtgttcac
     3361 atcagaaagt tattgtgaga ttagcaatga tataaggtgt gttaatgtca gtgagagtgg
     3421 tggcacagta cctatgaaac cgtgataaca agcggtggag ggatagccac tagtcatatg
     3481 aagaatagaa ttactaagaa aaatggtatg ggttcgagta agactaagag atgtacttgc
     3541 tcctgcaaac aaggagggta cagaccatgc taaagaaaaa caagctccgc tataacgagt
     3601 attatgatat gcaacatatt tatgacgagt tatacgcaca gagcaagaac ggtaataact
     3661 tttataagtt acttgaaatt attggttcgg aacaaaatat ttgtttagct taccgtaatc
     3721 tgaaatcaaa cagtggcagt aaaacggctg gaacggatgg aatgacgata gacgatataa
     3781 aacaactcag caatgctgag attgttgcta ccgttcggga aagccttagc aactaccgcc
     3841 ccaaatcagt cagacgtgtt ttcattccaa aagctgggag cgataaaatg cgtccattgg
     3901 ggattccgtg tatctgggac aggctggtac aacaatgtat cctgcaagtc ctagagccga
     3961 tatgcgaacc aaagttccat aatcattcct atggattccg tgcgaatcga agtgcccacc
     4021 atgcagtcag cagagtaacg acactgataa atttatcaaa atatcattac tgtgttgacg
     4081 tagacattaa aggatttttc gataatgtca atcatggaaa actattaaaa caaatctgga
     4141 cacttggtat tcgggataaa agattgattt gcattatcag caaaatgctg aaagccgaaa
     4201 ttgacggcga gggtgtccca gaaaaaggaa cgccacaagg cggtctgtta agtccactgc
     4261 tctccctcat agtccttaat gaattagact ggtgggtaag cagtcagtgg gaaacattcc
     4321 agccgaagaa ccgcagtaaa aatgggtggc ttcaatacgc aaagaaatat accaaactga
     4381 aaagcggttt tattgtacgt tacgctgatg attttaaaat catgtgttca acctatggag
     4441 aagcacaaag attttaccac agtaccgtag attttcttaa taaaaggctg aaattagaaa
     4501 tcagtccaga gaaatcaaag gtagtaaatc tgaagaaaaa ttcatcagat tttctggggt
     4561 tcaaaatcaa agtcattcca aaaggaagaa cgaaacacgg ttatgttgcc aaaacagata
     4621 tgaaccaaaa agcattaaag aaagcgaaaa caaatctaaa gttaaaggta aaagatatag
     4681 ccagacatac gactggcttc aatatcagca gatataatct gacagttatc ggtatgcaga
     4741 attactactg catagcaact aacgtatata acaacctgac agaagtaagt tacgctctct
     4801 taccgaccat acgaatccgt ttaaggaaca ttgcaaaatc agttccattt gaaagtacaa
     4861 gttcagaatt tcagagcagg acaaaaggaa tcagaccaaa aacaaaaata gtgatgattg
     4921 cggataatcc gctattgccg attcaaggag tacaacacaa aaatcctatg aatttctcgc
     4981 aagatatttg taattttaca aagcaaggga gaaacaaggt gcatgaggac gttgtagttg
     5041 taacaaaaga agaaatccga gcgttgttgg aaaatgaaaa ccctgccgat agcgtagagt
     5101 ttaacgacaa tcgtatttcg gcatatatcg cacaacaagg caactgctat gtaatgaatc
     5161 gtcgaggaac tccgtccact ctgatatgta ttcacaaatc agacaaagga gataaccttg
     5221 acagatattc taatctggca tttgtggaaa ttcccattgc aaaggcaatc ctaacagaat
     5281 cagcagacga agcaaaatca ctgttaaaag gttatgttct taatagtcag cagaagaaaa
     5341 aactgaacag aatacggacg aattacggat accagacaat aactgggaaa taacttataa
     5401 atctatttgg atatgatgga acgccgtatg aagcgaaagt ttcatgtacg gtgtgaagcg
     5461 ggggaaaact tggagataac atcaaaaagt tacctatcgc tataacgtgg cagatggaaa
     5521 gaaaccttgc cagagatttc ccatgaaatc aatatcgtca ctctgacttc tgatgagaaa
     5581 aacaaaggct tacttgaccc ttatgtgatt atgaaaaatc ccaaagattc tgaatcactg
     5641 gctattgata ttctgacatt ccttacgggg atttcctctc gtgatgggga acgcttccca
     5701 atccttagaa aagccattcg tgcagtaacc aatagtgaag tacgagggtt gatgaaagtg
     5761 attgaggaat tacgggttga gaatacgcca ctaagtacca gtatagccga ccatatcgaa
     5821 agttttacag actatgactt tgcacattta ttattcagta atggttatgt ggagcagtct
     5881 atcagcttag aaaaacaact gaacattata caggttgcgg acttggtact tcccgacaag
     5941 gaaacttcct ttgaggaata taccactatg gagcttttat ccgttgctat gctgattgtc
     6001 attagtacct ttgctttaga ctttatccat acagaccgaa gcattttcaa gattgtagat
     6061 ttagacgaag catggagctt tttacaggta gcacaaggaa aaacactatc tatgaagctg
     6121 gttcgggctg gtcgtgctat gaacgctggg gtatatttcg tgacccaaaa tacagacgac
     6181 ctcttagatg aaaaactgaa aaataacctc ggcttaaaat ttgcatttcg ttccactgac
     6241 cttaacgaga ttaaaaagac cttagccttt tttggtgtag acccagagga cgaaaacaat
     6301 cagaagcgat tgcgtgattt ggaaaacggg caatgcctta tcagtgattt atatggtcgt
     6361 gtcggtgtga tacagttcca ccctgtattt gaagaactgc tccatgcctt tgataccaga
     6421 ccacctgtgc gaaaagaggt gtaaatgtga aaccatcaat agtaaacaga ataaaatcaa
     6481 actggacgct gaaacgtcta ggtaaagtgg caatgacagt ggctttcaca cttgtgattg
     6541 ccatttttct tttagccatg ctgggaacgg tggttcaagc tgcgggcttg gtagatgata
     6601 cggtcaatgt ggcaaatgaa tacagccgat acccacttga aaactatcaa ctggattttt
     6661 atgtggataa tagctggggc tggcttccgt ggaactggtc ggacgggatt ggaaaacagg
     6721 tcatgtatgg actatatgcc attaccaatt ttatttggac aatcagtttg tatgtttcca
     6781 atgcgacagg ttacttagta caggaagcct attccttaga cttcatttcc gctacagcag
     6841 attccattgg taagaatatg cagaccttag ctggtgtgag tgcaaacgga ttttcaacag
     6901 agggtttcta tgttggattc ctcttactct tgattttggt tcttggggtt tatgttgcct
     6961 atacgggact gataaagaga gaaaccacaa aggcaattca tgccattatg aattttgtgc
     7021 tggtgtttat cctatcggct tcctttattg cctacgctcc cgactacatt aaaaaaatca
     7081 atgacttttc atcagacatc agtaatgcca gtttatcact tggcacgaag attgtcatgc
     7141 cccattccga tagtcaaggc aaggacagcg tggacttaat cagagatagc ctgttttcca
     7201 tacaggttca gcaaccgtgg ctactgcttc aatacaacag ttcagacatt gaaagtatcg
     7261 gtattgaccg tgtggaaagc ctgctctcca ccagcccaga ttccaacaat ggcgaagaca
     7321 gagaaaaaat tgttgcggaa gaaattgaag acagaagcaa taccaatcta accattacaa
     7381 agaccattaa ccgtttaggt acagtcttct tcctatttgt cttcaatatt gggatttcca
     7441 tatttgtatt cctattaaca ggaatcatga ttttctcgca ggtacttttt atcatctatg
     7501 ctatgtttct gcctgtgagc tttattttaa gcatgattcc atcatttgat ggtatgtcaa
     7561 aacgagccat aacaaagctc tttaatacca ttttgacacg agctggaatc acattgatta
     7621 ttacgacagc atttagtatt tcaaccatgc tctatacctt atcggctggt tatccgttct
     7681 ttttgattgc ttttctacag attgtgacct ttgcaggaat ctacttcaag ctgggcgatt
     7741 taatgagtat gttttctcta cagagtaacg attctcaaag tgtgggaagt cgtgtgatga
     7801 gaaaacctcg tatgcttatg cacgctcaca tgcaccgtct acagcggaaa cttggacgtt
     7861 ccatgactac tctaggggct gggtctgcca ttgttacagg taaaaaagga cagtcgggtt
     7921 cggggagttc tgcaaggaca caagcagatc actcccgacc agacggaaag gaaaaatcaa
     7981 cacttggaaa acgtatcggt caaaccatcg gtacagtagc tgataccaaa gacagaatgg
     8041 tagacactgc tagtggtttg aaagaacagg ttaaagattt gccgaccaat gcaagatatg
     8101 cagtatatca aggaaaatcc aaagtaaaag agaatgtccg tgatttaacc agtagtattt
     8161 ctcaaaccaa agcggacaga gccagtggac gcaaggaaca gcaggaacaa aggcgaaaaa
     8221 ccattgcgaa gcgtcgctct gaaatggaac aggtcaaaca gaaaaaacag cctgcttctt
     8281 ctgttcatga aagaccgact acaagacaag aacaatatca tgatgaacag acctcaaaac
     8341 agtctaatat tcagacttca tataaggaat ctcaacaagc caaacaagag cgtccagcag
     8401 ttaagtccga tttttcaagt ccaaaagtgg aacgccaagg caataccgtt caagaaaaaa
     8461 ccgttcaaaa gccagcaact tcaaccacta cagcagatag aacttcacaa cgtccaatca
     8521 caaaagaacg tccgtctact gttcaaagag taccactaca aaatacaaga agtagaccac
     8581 caatcaaaac cgccaccatt aagaaagtcg gtaagaaacc atgaagttga aaactttagt
     8641 gattggtggt tctggattat tcttgatggt cttctcactg cttctgtttg ttgccatttt
     8701 attttcagat gaacaggaca gcggaatttc caatattcat tatggaggtg tgaatgtttc
     8761 cgcagaagtg ctggctcata agcctatggt agaaaaatat gccaaagaat atggcgttga
     8821 agaatatgtc aacatacttc ttgcgattat acaggtggaa tcgggcggta ctgcggaaga
     8881 tgttatgcag tcctcggaat ccctcggtct tccacctaat tcattgagta cagaagaatc
     8941 cattaagcaa ggtgtgaagt atttcagtga attattagcc agtagcgaaa ggctcagtgt
     9001 agatttagaa tcggttatcc agtcctacaa ttatggtggt ggtttcttag ggtatgtggc
     9061 taatcgtgga aataaatata cctttgaact ggctcaaagt ttctcaaaag agtattcagg
     9121 tggcgaaaaa gtgtcttacc ccaatcccat agccatacct atcaatgggg gctggcgata
     9181 caactatggc aatatgtttt atgtgcaact ggtaacgcag tatcttgtca caacagagtt
     9241 tgatgatgat acggtacaag ccatcatgga cgaagcactg aaatatgagg gctggcgata
     9301 cgtttacggt ggagcttccc cgactacttc ttttgattgt agcggactga cacaatggac
     9361 gtatggaaaa gctggaatta acttaccacg aaccgcacaa cagcaatatg atgtgaccca
     9421 gcatatccca ctatcggaag cacaagctgg cgatttggtt ttctttcatt ctacctataa
     9481 cgctggctct tatattactc atgttgggat ataccttggc aataaccgta tgtttcatgc
     9541 aggcgaccca atcggttatg ccgacttaac aagcccctac tggcaacagc atttagtggg
     9601 agcaggacga atcaaacaat gagaaaggaa gatttaatga tgaaatttag aaaaaatcag
     9661 aataaagaaa aacagatacc aaaggaaaag aaacctcgtg tctataaggt caatcctcat
     9721 aaaaaggttg tgattgcctt gtgggtactt ttagggctta gtttcagctt tgcgatattc
     9781 aagcacttta cagctataga tactcatact attcacgaaa caactatcat agaaaaggaa
     9841 tacgttgata ctcatcatgt agaaaatttt gtagagaact ttgcgaaagt ctactattca
     9901 tgggagcaat ccgataagtc cattgataat cgaatggaaa gtctaaaagg ctatctgaca
     9961 gatgaacttc aagctctcaa tgttgataca gtacgcaaag atattcctgt atcgtcttct
    10021 gtaagaggat ttcagatatg gacggtagag ccaactggcg acaatgagtt taatgtaacc
    10081 tacagtgtag accagctcat tacagaggga gaaaatacaa agaccgtcca ctctgcttat
    10141 atagtgagtg tctatgtaga tggttctgga aatatggtac tggttaagaa tccgaccatt
    10201 accaacatac ctaagaaatc aagttataaa ccaaaagcca ttgaaagtga ggggacggtt
    10261 gattccatta caaccaatga aatcaatgag tttttaacga cgttcttcaa gctctatcct
    10321 acagcgacag ccagtgaact ttcctactat gtgaatgacg ggatattaaa accaatcgga
    10381 aaagagtaca tctttcaaga actggtaaat cctattcaca atcgtaagga taatcaagtc
    10441 acggtatcgc tgacagtgga gtatatcgac cagcagacca aagcaacgca ggtatctcaa
    10501 tttgatttgg tacttgaaaa gaacgggagt aattggaaga ttatagaata acaaatattg
    10561 gtacattatt acagctattt tgtaatcacg tactctcttt gataaaaaat tggagattcc
    10621 tttacaaata tgctcttacg tgctattatt taagtatcta tttaaaagga gttaataaat
    10681 atgcggcaag gtattattaa ataaactgtc aatttgatag cgggaacaaa taattggatg
    10741 tcctttttta ggagggctta gttttttgta cccagtttaa gaataccttt atcatgtgat
    10801 tctaaagtat ccggagaata tctgtatgct ttgtatgcct atggttatgc ataaaaatcc
    10861 cagtgataag agtatttatc actgggattt ttatgccctt ttgggctttt gaatggagga
    10921 aaatcacatg aaaattatta atattggagt tttagctcat gttgatgcag gaaaaactac
    10981 cttaacagaa agcttattat ataacagtgg agcgattaca gaattaggaa gcgtggacaa
    11041 aggtacaacg aggacggata atacgctttt agaacgtcag agaggaatta caattcagac
    11101 aggaataacc tcttttcagt gggaaaatac gaaggtgaac atcatagaca cgccaggaca
    11161 tatggatttc ttagcagaag tatatcgttc attatcagtt ttagatgggg caattctact
    11221 gatttctgca aaagatggcg tacaagcaca aactcgtata ttatttcatg cacttaggaa
    11281 aatggggatt cccacaatct tttttatcaa taagattgac caaaatggaa ttgatttatc
    11341 aacggtttat caggatatta aagagaaact ttctgccgaa attgtaatca aacagaaggt
    11401 agaactgtat cctaatgtgt gtgtgacgaa ctttaccgaa tctgaacaat gggatacggt
    11461 aatagaggga aacgatgacc ttttagagaa atatatgtcc ggtaaatcat tagaagcatt
    11521 ggaactcgaa caagaggaaa gcataagatt tcagaattgt tctctgttcc ctctttatca
    11581 tggaagtgca aaaagtaata tagggattga taaccttata gaagttatta ctaataaatt
    11641 ttattcatca acacatcgag gtccgtctga actttgcgga aatgttttca aaattgaata
    11701 tacaaaaaaa agacaacgtc ttgcatatat acgcctttat agtggagtac tacatttacg
    11761 agattcggtt agagtatcag aaaaagaaaa aataaaagtt acagaaatgt atacttcaat
    11821 aaatggtgaa ttatgtaaga ttgatagagc ttattctgga gaaattgtta ttttgcaaaa
    11881 tgagtttttg aagttaaata gtgttcttgg agatacaaaa ctattgccac agagaaaaaa
    11941 gattgaaaat ccgcaccctc tactacaaac aactgttgaa ccgagtaaac ctgaacagag
    12001 agaaatgttg cttgatgccc ttttggaaat ctcagatagt gatccgcttc tacgatatta
    12061 cgtggattct acgacacatg aaattatact ttctttctta gggaaagtac aaatggaagt
    12121 gattagtgca ctgttgcaag aaaagtatca tgtggagata gaactaaaag agcctacagt
    12181 catttatatg gagagaccgt taaaaaatgc agaatatacc attcacatcg aagtgccgcc
    12241 aaatcctttc tgggcttcca ttggtttatc tgtatcaccg cttccgttgg gaagtggaat
    12301 gcagtatgag agctcggttt ctcttggata cttaaatcaa tcatttcaaa atgcagttat
    12361 ggaagggata cgctatggtt gtgaacaagg attgtatggt tggaatgtga cggactgtaa
    12421 aatctgtttt aagtatggct tatactatag ccctgttagt accccagcag attttcggat
    12481 gcttgctcct attgtattgg aacaagtctt aaaaaaagct ggaacagaat tgttagagcc
    12541 atatcttagt tttaaaattt atgcgccaca ggaatatctt tcacgagcat acaacgatgc
    12601 tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa aataatgagg tcattcttag
    12661 tggagaaatc cctgctcggt gtattcaaga atatcgtagt gatttaactt tctttacaaa
    12721 tggacgtagt gtttgtttaa cagagttaaa agggtaccat gttactaccg gtgaacctgt
    12781 ttgccagccc cgtcgtccaa atagtcggat agataaagta cgatatatgt tcaataaaat
    12841 aacttagtgt attttatgtt gttatataaa tatggtt
//
LOCUS       DAMYXM010000234         8163 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.guided.097, whole genome shotgun sequence.
ACCESSION   DAMYXM010000234 DAMYXM010000000
VERSION     DAMYXM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.guided.097"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(134..577)
                     /locus_tag="PNS21_003019"
     CDS             complement(134..577)
                     /locus_tag="PNS21_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDI5160630.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="PNS21_003020"
     CDS             complement(795..2135)
                     /locus_tag="PNS21_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI5160631.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="PNS21_003021"
     CDS             2404..3300
                     /locus_tag="PNS21_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDI5160632.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="PNS21_003022"
     CDS             complement(3450..4391)
                     /locus_tag="PNS21_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDI5160633.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="PNS21_003023"
     CDS             4517..5638
                     /locus_tag="PNS21_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDI5160634.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="PNS21_003024"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="PNS21_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDI5160635.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="PNS21_003025"
     CDS             7426..7992
                     /locus_tag="PNS21_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDI5160636.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         2710 a         1514 c         1606 g         2333 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tag
//
LOCUS       DAMYXM010000235         3814 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.guided.132, whole genome shotgun sequence.
ACCESSION   DAMYXM010000235 DAMYXM010000000
VERSION     DAMYXM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.guided.132"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            183..1250
                     /gene="rlmN"
                     /locus_tag="PNS21_003026"
     CDS             183..1250
                     /gene="rlmN"
                     /locus_tag="PNS21_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDI5160637.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(1443..2546)
                     /locus_tag="PNS21_003027"
     CDS             complement(1443..2546)
                     /locus_tag="PNS21_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDI5160638.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            3099..3647
                     /gene="aac(6')"
                     /locus_tag="PNS21_003028"
     CDS             3099..3647
                     /gene="aac(6')"
                     /locus_tag="PNS21_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDI5160639.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1247 a          648 c          764 g         1155 t
ORIGIN      
        1 atcaattctg attcatcagt cccataaatt atagagccat cttttctaat acggtatgat
       61 gtcttgtctt tttagttttt gtgagattaa agttctattc gatttatttg tgaagtattg
      121 cattaaggaa atgaatcatg tatagtatgt agcgaaactt tgagaaaaag agggaaaata
      181 ctttggaaaa acagtccatt tatggcttga cgaatgaaga attaatcaat tggtttatag
      241 aaaacggcga gaaaaaattc cgagctgccc aagtttggga atggttgtat caaaaacgtg
      301 taagtaattt cacagaaatg acgaatctat cgaaacaatt gatcgaaaaa ttatcggagc
      361 attttatcat caatcctcta aaacagatgg tcgtccaaga agcttcggat ggtacagtaa
      421 aatatttgtt tgaactccct gataaaaaca tgattgagac tgtactgatg agacaagaat
      481 atggcttgtc agtttgtgtg acgacacaag taggatgtaa tatcggctgt acgttttgtg
      541 caagcgggct gctcaaaaag aaccgtgatc tgactgctgg agaaattgtt gcacagatca
      601 tgatggtcca acattatttt gatgagcgca atcttggcga acgcgtttca catgtcgttg
      661 taatggggat cggtgagcca tttgataatt acgacaatgt aatggacttt ttgcatatca
      721 tcaatgatgc caaaggattg gcaattggtg ctcggcacat tacagtttcc actagtggat
      781 tagcacacaa aatcaaggaa ttcgctaata acggcttgca agtcaatctg gctatttcct
      841 tgcacgcgcc gaataatgag gtgcgaacat cgatcatgcg gatcaaccga agtttcccaa
      901 ttgagaaatt gatggaagct gtggatgagt atttagagaa aacaaaccgt cgaatcacgt
      961 ttgaatatat catgttgaat caagtaaatg atagaccaga gcatgcgcaa caattagcag
     1021 atctattaaa agataagaaa aaattagcat atgtcaactt gattccatat aatccagtca
     1081 gtgaacatga tcaatattct cgcagtccaa aagctgatgt attgagattt tatgatgtat
     1141 tgaagaaaaa cggcgtaaac tgtgtgatac gtaaagaaca tggtacagat attgatgcag
     1201 cttgcggaca attgcgaagc aagcaaatga aaaaaacagt tgccaaataa tcgtatccta
     1261 ctcgtttttt caattaccat aaaaggagca gttttcttaa ttaaggaact gttcaactta
     1321 aataagagaa aagattctat agcgaaagct ataaagcttt ttttgacaag aaaaaccctc
     1381 atcaagcaaa gaaatgtcct caatttgatg acagcctgct tgatgggggt tttagcaatt
     1441 atttaactga ttttcttata tccttgctgg ctttcttgtg tagcatgata agtttctacg
     1501 tcaagaatgg cctgtttaaa tttttctcgg gtgatttcat aaggcacatg tttaatatca
     1561 tttgttgcca tggctttatc taagattgct tctaaatcgg tcgtgacatt gagattcatg
     1621 tcggttaacg atactggaag ttggttcttt ttcataaaca catagatttt atcaaacaaa
     1681 tcgaactgct gatccatcat caataaggtc agtacaccat aactgacaga tgctccgtga
     1741 aggtatccct ccattttttc tagaacagtc gtaccgtaat agaatgagtg tgccatagca
     1801 gaattatagt tattgttaac taaaacagaa accaatccgg tagtaacaat gatatccgaa
     1861 acgacttgtt caattgcata tgagggacga tcgtcggcac tatctttcaa tgcttgcgta
     1921 ccataagtca agagacgatc aatgcacatt tttccgattt ggattcctaa tctatccgtg
     1981 tagcctagat catcgccacg agcagaaaaa gtagattcga cttctttgga taaagcatcg
     2041 ccaatacctg cccataagta tttatcaggt gcttcggcaa taattgctgt atcaatgaag
     2101 gtgtgtacag gtggttttga aggaaagaag agtccgtcca ttacgccttc ttcgttatag
     2161 atgacacata ccgcagtgac gggcgaacag ttcgaaccaa tagtgggaaa ggtgaagact
     2221 ggtttagcta gtttttcacc aatgactttt acagtatcgc aagcacggcc accaccgact
     2281 gcaaacagga cgtctgcttt ttggacactt tcatgctgga tcaactgttc aatatttcca
     2341 taagttgaat tacccccgta ccagagactg gcgacaacct ctatttcctg tgtcaaagct
     2401 acttttattt tgttttctgc ctttgaaagt gcagtcttcc ctccaatcag cactactttt
     2461 gtcccatatt ctttgacgat tccatttgct ttttcatacg catctgttcc aatggtataa
     2521 tttggtaaga aaacactttg actcatattc atttccttct ttcttcgttg aaacgggtta
     2581 aaattttaaa gagtgatcga tctgactgcg gctgtagatg gagtagaggt gaacctaaga
     2641 caggataagt gatgaataag gtggcatgaa caccttgaaa tcatcaataa gtatcctaaa
     2701 aacgaacaag tacaaaaaca ttgaaaacga caataatgaa tttttgttcc tcttagtatt
     2761 tctaccgatt ccctttgctt ttttgtaatt atctaaaaat tctaaaaatt aagattatta
     2821 tatgaaaaca ggttttattt gtcaattcat aattggtttt ttttaaagca aaagataatc
     2881 tatatcatga tgaacaaaca gagttttttc ctccaattga tggctttcct gatctttcct
     2941 attttgaata aagtgtttct gtataatttt tttattttat aagtaagata aataatgaaa
     3001 gaatagagat actgattggt agctaaagat ttttgagcga gaacatttcg ttttttcaaa
     3061 aggactgtta gaataagcaa gaacaagggg gaagacgtat gataatcagt gaatttgacc
     3121 gtaataatcc agtattgaaa gatcagcttt ctgatttact gagactgact tggccggaag
     3181 aatatggaga cagctcggca gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg
     3241 tagcagcggt agaccaagat gagttagtag gatttattgg tgcaatccct caatacggta
     3301 tcacaggttg ggaattgcat ccattagttg tagaaagctc ccgacgaaag aaccaaatag
     3361 gtactcgatt agtcaattac ttagaaaaag aagtagcttc cagaggagga atcacgattt
     3421 atttaggtac ggatgattta gaccatggaa caacgttaag tcaaacggac ctgtatgagc
     3481 atacatttga taaagtggct tccatccaga accttcgtga acatccgtat gaattctatg
     3541 aaaaattagg ttataaaatc gtaggtgtct taccaaatgc aaatggctgg gacaaaccgg
     3601 atatttggat ggcaaaaacg attattcctc gaccaaattc tcaataataa aaagaggttg
     3661 tgacagaagt aatcagcttc aagaaataag aaggaattta cgaaaattac ttcttgaatt
     3721 tttgtgaaat tccaacttat ttccgaagaa gctacttttg gaacaccgtg taccagaggt
     3781 tgtgaaaaag tcgcttagtt tcgagtagta agaa
//
LOCUS       DAMYXM010000236         1500 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.guided.182, whole genome shotgun sequence.
ACCESSION   DAMYXM010000236 DAMYXM010000000
VERSION     DAMYXM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.guided.182"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            390..473
                     /locus_tag="PNS21_003029"
     CDS             390..473
                     /locus_tag="PNS21_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HDI5160640.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            598..1335
                     /gene="erm(B)"
                     /locus_tag="PNS21_003030"
     CDS             598..1335
                     /gene="erm(B)"
                     /locus_tag="PNS21_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDI5160641.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          550 a          223 c          245 g          482 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattaaaa attttgtata ataggaattg aagttaaatt agatgctaaa
      361 aatttgtaat taagaaggag ggattcgtca tgttggtatt ccaaatgcgt tatgtagata
      421 aaacatctac tgttttgaaa cagactaaaa acagtgatta cgcagataaa taaatacgtt
      481 agattaattc ctaccagtga ctaatcttat gactttttaa acagataact aaaattacaa
      541 acaaatcgtt taacttctgt atttgtttat agatgtaatc acttcaggag tgattacatg
      601 aacaaaaata taaaatattc tcaaaacttt ttaacgagtg aaaaagtact caaccaaata
      661 ataaaacaat tgaatttaaa agaaaccgat accgtttacg aaattggaac aggtaaaggg
      721 catttaacga cgaaactggc taaaataagt aaacaggtaa cgtctattga attagacagt
      781 catctattca acttatcgtc agaaaaatta aaactgaata ctcgtgtcac tttaattcac
      841 caagatattc tacagtttca attccctaac aaacagaggt ataaaattgt tgggagtatt
      901 ccttaccatt taagcacaca aattattaaa aaagtggttt ttgaaagcca tgcgtctgac
      961 atctatctga ttgttgaaga aggattctac aagcgtacct tggatattca ccgaacacta
     1021 gggttgctct tgcacactca agtctcgatt cagcaattgc ttaagctgcc agcggaatgc
     1081 tttcatccta aaccaaaagt aaacagtgtc ttaataaaac ttacccgcca taccacagat
     1141 gttccagata aatattggaa gctatatacg tactttgttt caaaatgggt caatcgagaa
     1201 tatcgtcaac tgtttactaa aaatcagttt catcaagcaa tgaaacacgc caaagtaaac
     1261 aatttaagta ccgttactta tgagcaagta ttgtctattt ttaatagtta tctattattt
     1321 aacgggagga aataattcta tgagtcgctt ttgtaaattt ggaaagttac acgttactaa
     1381 agggaatgta gataaattat taggtatagg gcacctctaa taactaccaa ttaattcacc
     1441 tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc tatttcttat cgatacaaat
//
LOCUS       DAMYXM010000237         1266 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.guided.195, whole genome shotgun sequence.
ACCESSION   DAMYXM010000237 DAMYXM010000000
VERSION     DAMYXM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.guided.195"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..1175
                     /locus_tag="PNS21_003031"
                     /note="aph(2'')-Ia"
     CDS             <1..1175
                     /locus_tag="PNS21_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HDI5160642.1"
                     /translation="KTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKY
                     LIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNT
                     NLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLR
                     QMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFE
                     GKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGE
                     DILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          543 a           95 c          214 g          414 t
ORIGIN      
        1 caaaaactga tgagatagtc tatggtatgg atcaatttat aggagagcca aattattgga
       61 gtaaaggaat tggtacaaga tatattaaat tgatttttga atttttgaaa aaagaaagaa
      121 atgctaatgc agttatttta gaccctcata aaaataatcc aagagcaata agggcatacc
      181 aaaaatctgg ttttagaatt attgaagatt tgccagaaca tgaattacac gagggcaaaa
      241 aagaagattg ttatttaatg gaatatagat atgatgataa tgccacaaat gttaaggcaa
      301 tgaaatattt aattgagcat tactttgata atttcaaagt agatagtatt gaaataatcg
      361 gtagtggtta tgatagtgtg gcatatttag ttaataatga atacattttt aaaacaaaat
      421 ttagtactaa taagaaaaaa ggttatgcaa aagaaaaagc aatatataat tttttaaata
      481 caaatttaga aactaatgta aaaattccta atattgaata ttcgtatatt agtgatgaat
      541 tatctatact aggttataaa gaaattaaag gaactttttt aacaccagaa atttattcta
      601 ctatgtcaga agaagaacaa aatttgttaa aacgagatat tgccagtttt ttaagacaaa
      661 tgcacggttt agattataca gatattagtg aatgtactat tgataataaa caaaatgtat
      721 tagaagagta tatattgttg cgtgaaacta tttataatga tttaactgat atagaaaaag
      781 attatataga aagttttatg gaaagactaa atgcaacaac agtttttgag ggtaaaaagt
      841 gtttatgcca taatgatttt agttgtaatc atctattgtt agatggcaat aatagattaa
      901 ctggaataat tgattttgga gattctggaa ttatagatga atattgtgat tttatatact
      961 tacttgaaga tagtgaagaa gaaataggaa caaattttgg agaagatata ttaagaatgt
     1021 atggaaatat agatattgag aaagcaaaag aatatcaaga tatagttgaa gaatattatc
     1081 ctattgaaac tattgtttat ggaattaaaa atattaaaca ggaatttatc gaaaatggta
     1141 gaaaagaaat ttataaaagg acttataaag attgattata taatatatga aaagctatta
     1201 taaaagacat tagtattaaa tagtttaaaa aaattcaaaa tctttcagag aagctgttaa
     1261 aggaat
//
LOCUS       DAMYXM010000238         1148 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.guided.200, whole genome shotgun sequence.
ACCESSION   DAMYXM010000238 DAMYXM010000000
VERSION     DAMYXM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.guided.200"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            47..439
                     /locus_tag="PNS21_003032"
     CDS             47..439
                     /locus_tag="PNS21_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI5160643.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            440..>1148
                     /gene="aac(6')-Ie"
                     /locus_tag="PNS21_003033"
     CDS             440..>1148
                     /gene="aac(6')-Ie"
                     /locus_tag="PNS21_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HDI5160644.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKG"
BASE COUNT          461 a           92 c          205 g          390 t
ORIGIN      
        1 aacattgtat agcattttac acaggagtct ggacttgact gagtttatgg aagaagtttt
       61 aattgatgat aatatggttt ttgatattga taatttaaaa ggatttctta atgataccag
      121 ttcatttggg tttatagcta aagaaaataa taaaattata ggatttgcat attgctatac
      181 acttttaaga cctgatggaa aaacaatgtt ttatttacac tcaataggaa tgttacctaa
      241 ctatcaagac aaaggttatg gttcaaaatt attatctttt attaaggaat attctaaaga
      301 gattggttgt tctgaaatgt ttttaataac tgataaaggt aatcctagag cttgccatgt
      361 atatgaaaaa ttaggtggta aaaatgatta taaagatgaa atagtatatg tatatgatta
      421 tgaaaaaggt gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat
      481 agatgatgat tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta
      541 tggtggtaga gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg
      601 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
      661 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
      721 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
      781 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
      841 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
      901 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt
      961 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
     1021 gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag
     1081 tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa
     1141 aaaaggtt
//
LOCUS       DAMYXM010000239          572 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022225
            SAMN29549536-rid16865173.guided.224, whole genome shotgun sequence.
ACCESSION   DAMYXM010000239 DAMYXM010000000
VERSION     DAMYXM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549536
            Sequence Read Archive: SRR20032558
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001567830.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001567830.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2023 06:58:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 37 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549536-rid16865173.guided.224"
                     /strain="AUSMDU00022225"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            16..513
                     /gene="dfrG"
                     /locus_tag="PNS21_003034"
     CDS             16..513
                     /gene="dfrG"
                     /locus_tag="PNS21_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDI5160645.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT          214 a           65 c          125 g          168 t
ORIGIN      
        1 aaggctgtgt tataaatgaa agtttctttg attgctgcga tggataagaa tagagtgatt
       61 ggcaaagaga atgacattcc ttggaggatt cccaaggact gggaatatgt taaaaatact
      121 acaaagggac atccgataat attaggtagg aagaaccttg aatcaatcgg aagagcctta
      181 cctgacagaa gaaatattat tctgacgaga gataaggggt ttacctttaa tggttgtgaa
      241 attgttcatt caatagaaga tgtttttgag ttatgtaaaa acgaagaaga aatttttatt
      301 ttcggaggag aacagattta taatttgttt ttcccttatg ttgagaaaat gtacatcaca
      361 aaaatacatc atgaattcga aggagatact ttttttccag aagtgaatta tgaggaatgg
      421 aatgaggtat ttgcccaaaa agggataaag aatgataaaa atccgtataa ctactatttt
      481 catgtatatg aaagaaaaaa cttattgagt taacgggtgc gttaattgaa gaagaacagt
      541 aaagataaag cacaagagct tgggattact cc
//
